ECD cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	ECD
Entrez Gene ID	11319
Full Name	ecdysoneless cell cycle regulator
Synonyms	GCR2,HSGT1,SGT1
General protein information	Preferred Names ecdysoneless cell cycle regulator Names protein ecdysoneless homolog ecdysoneless homolog human suppressor of GCR two protein SGT1 suppressor of GCR2 suppressor of S. cerevisiae gcr2
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 10 Map Location: 10q22.2
Disorder MIM:	616464

mRNA and Protein(s)

mRNA	Protein	Name
NM_007265.2	NP_009196.1	protein ecdysoneless homolog isoform 1
NM_001135752.1	NP_001129224.1	protein ecdysoneless homolog isoform 2
NM_007265.3	NP_009196.1	protein ecdysoneless homolog isoform 1
NM_001135753.1	NP_001129225.1	protein ecdysoneless homolog isoform 3

Pathways from BioSystems

Homologies

	ECD	XP_001103459.1
Canis lupus familiaris (dog)	ECD	XP_536386.1
Bos taurus (cattle)	ECD	NP_001039736.1
Rattus norvegicus (Norway rat)	Ecd	XP_006251737.1
Arabidopsis thaliana (thale cress)	AT5G65490	NP_201352.1
Oryza sativa (rice)	Os08g0447200	NP_001061930.1
Homo sapiens (human)	ECD	NP_009196.1
Pan troglodytes (chimpanzee)	ECD	XP_001141366.2
Gallus gallus (chicken)	ECD	NP_001186453.1
Mus musculus (house mouse)	Ecd	NP_081751.1
Drosophila melanogaster (fruit fly)	ecd	NP_647707.1
Danio rerio (zebrafish)	ecd	NP_956185.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005814	XP_315835.4
Xenopus tropicalis (tropical clawed frog)	ecd	XP_002936899.2

Gene Ontology
Bibliography

Related articles in PubMed

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

A Novel Interaction of Ecdysoneless (ECD) Protein with R2TP Complex Component RUVBL1 Is Required for the Functional Role of ECD in Cell Cycle Progression.
Mir RA, Bele A, Mirza S, Srivastava S, Olou AA, Ammons SA, Kim JH, Gurumurthy CB, Qiu F, Band H, Band V
Molecular and cellular biology36(6)886-99(2015 Dec)

The cell cycle regulator ecdysoneless cooperates with H-Ras to promote oncogenic transformation of human mammary epithelial cells.
Bele A, Mirza S, Zhang Y, Ahmad Mir R, Lin S, Kim JH, Gurumurthy CB, West W, Qiu F, Band H, Band V
Cell cycle (Georgetown, Tex.)14(7)990-1000(2015)

Phosphorylation-dependent PIH1D1 interactions define substrate specificity of the R2TP cochaperone complex.
Ho?ej?? Z, Stach L, Flower TG, Joshi D, Flynn H, Skehel JM, O'Reilly NJ, Ogrodowicz RW, Smerdon SJ, Boulton SJ
Cell reports7(1)19-26(2014 Apr)

The Salmonella type III effector SspH2 specifically exploits the NLR co-chaperone activity of SGT1 to subvert immunity.
Bhavsar AP, Brown NF, Stoepel J, Wiermer M, Martin DD, Hsu KJ, Imami K, Ross CJ, Hayden MR, Foster LJ, Li X, Hieter P, Finlay BB
PLoS pathogens9(7)e1003518(2013)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The interaction of ECD with RUVBL1, and its CK2-mediated phosphorylation, independent of its interaction with PIH1D1, are important for its cell cycle regulatory function.
Title: A Novel Interaction of Ecdysoneless (ECD) Protein with R2TP Complex Component RUVBL1 Is Required for the Functional Role of ECD in Cell Cycle Progression.

Ecdysoneless and H-Ras expressing human mammary epithelial cells form tumors in NOD/SCID mice.
Title: The cell cycle regulator ecdysoneless cooperates with H-Ras to promote oncogenic transformation of human mammary epithelial cells.

TXNIP has a role in the pathway involving hEcd to increase p53 stability and activity
Title: TXNIP interacts with hEcd to increase p53 stability and activity.

Ecd is a novel tumor-promoting factor that is differentially expressed in pancreatic cancer and potentially regulates glucose metabolism within cancer cells.
Title: Overexpression of ecdysoneless in pancreatic cancer and its role in oncogenesis by regulating glycolysis.

Ecd as a novel marker for breast cancer progression and show that levels of Ecd expression predict poorer survival in Her2/neu overexpressing breast cancer patients.
Title: Overexpression of a novel cell cycle regulator ecdysoneless in breast cancer: a marker of poor prognosis in HER2/neu-overexpressing breast cancer patients.

The following ECD gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ECD cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu05925	NM_007265.3 Latest version!	Homo sapiens ecdysoneless cell cycle regulator (ECD), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu24432	NM_001135752.1 Latest version!	Homo sapiens ecdysoneless cell cycle regulator (ECD), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
OHu24433	NM_001135753.1 Latest version!	Homo sapiens ecdysoneless cell cycle regulator (ECD), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
View Old Accession Versions >>
OHu05925	NM_007265.2	Homo sapiens ecdysoneless cell cycle regulator (ECD), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ECD ( NM_007265.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_009196.1 Homo sapiens ecdysoneless cell cycle regulator (ECD), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu05925
Clone ID Related Accession (Same CDS sequence)	NM_007265.2 , NM_007265.3
Accession Version	NM_007265.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1935bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-19
Organism	Homo sapiens(human)
Product	protein ecdysoneless homolog isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BM763002.1, DA162516.1, D88208.1 and BC103720.1. On Oct 10, 2008 this sequence version replaced NM_007265.1. Transcript Variant: This variant (1) represents a more abundant transcript and encodes isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.141629.1, SRR1660803.180317.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGGAAGAAA CCATGAAGCT TGCTACGATG GAAGACACAG TGGAGTACTG CCTGTTCCTG 
ATACCAGATG AGTCAAGGGA CTCAGATAAA CATAAAGAGA TTCTTCAGAA GTACATTGAG 
AGAATAATCA CTCGGTTTGC ACCTATGCTG GTCCCCTACA TCTGGCAGAA TCAGCCTTTC 
AATCTTAAAT ATAAACCTGG GAAAGGAGGT GTTCCTGCTC ATATGTTTGG CGTGACAAAG 
TTTGGGGATA ACATTGAGGA TGAATGGTTT ATTGTTTATG TAATAAAGCA GATCACAAAG 
GAATTTCCAG AGTTAGTAGC AAGGATTGAA GACAATGATG GTGAATTCTT GTTAATAGAA 
GCTGCTGACT TTCTCCCTAA ATGGCTGGAT CCTGAAAATA GCACCAATAG GGTATTTTTC 
TGCCATGGGG AATTGTGTAT TATCCCTGCA CCAAGAAAAT CTGGAGCAGA ATCTTGGTTA 
CCCACCACAC CCCCAACAAT TCCACAAGCA TTGAATATAA TCACAGCACA TTCAGAAAAA 
ATACTTGCTT CAGAATCTAT ACGAGCTGCT GTGAATAGGC GCATCAGAGG GTACCCAGAA 
AAAATTCAGG CCTCACTTCA TCGAGCACAC TGCTTCCTTC CAGCTGGCAT TGTGGCAGTG 
CTAAAGCAGC GCCCCAGATT GGTGGCTGCA GCAGTCCAGG CATTTTACCT ACGAGACCCT 
ATTGACCTGC GAGCTTGTCG TGTTTTCAAG ACATTCTTGC CTGAAACACG AATAATGACA 
TCGGTCACAT TCACTAAATG TCTATATGCA CAATTGGTGC AACAAAGGTT TGTGCCAGAC 
CGGCGGAGTG GATACAGGCT GCCTCCTCCA TCTGATCCCC AGTACCGAGC CCATGAATTG 
GGCATGAAAT TGGCTCATGG ATTTGAGATC TTATGCTCCA AATGTAGCCC ACATTTTTCT 
GACTGCAAGA AATCCCTTGT GACTGCCTCA CCACTCTGGG CCAGTTTCCT TGAAAGTCTG 
AAAAAGAATG ATTACTTTAA GGGACTGATA GAAGGTTCTG CTCAGTACCG GGAAAGGCTA 
GAAATGGCAG AGAATTACTT CCAGCTCTCA GTAGACTGGC CAGAAAGTTC TCTTGCTATG 
AGCCCTGGTG AAGAAATCTT AACCTTATTA CAGACAATAC CATTTGATAT AGAAGACCTT 
AAGAAAGAAG CAGCTAATCT TCCCCCAGAG GATGATGACC AGTGGTTAGA TCTCTCACCA 
GATCAGCTGG ACCAGCTGCT GCAGGAAGCT GTTGGCAAAA AAGAATCCGA GTCTGTTTCC 
AAGGAGGAGA AGGAGCAGAA CTATGACTTA ACTGAAGTCT CAGAGAGCAT GAAAGCTTTC 
ATATCCAAAG TCTCAACCCA CAAGGGAGCA GAGCTGCCTC GAGAACCTTC TGAGGCTCCA 
ATCACTTTTG ATGCAGATTC TTTTCTTAAT TATTTTGATA AGATTTTAGG GCCAAGGCCT 
AATGAGTCAG ATTCTGATGA TCTGGATGAT GAAGACTTTG AATGTTTAGA TAGTGATGAT 
GACTTGGACT TTGAAACACA CGAACCTGGC GAAGAGGCTT CCCTGAAAGG AACACTTGAT 
AATCTCAAGT CATACATGGC CCAGATGGAC CAGGAACTAG CACACACCTG CATCAGCAAA 
AGTTTCACCA CTAGGAACCA AGTGGAACCT GTATCCCAGA CTACCGATAA CAATTCAGAT 
GAGGAAGATT CTGGTACGGG AGAATCTGTT ATGGCACCAG TAGATGTAGA CCTGAACCTG 
GTTTCAAATA TATTGGAATC CTATAGCTCC CAAGCTGGAC TGGCAGGACC TGCTTCCAAT 
CTTTTACAAA GCATGGGAGT GCAGCTGCCT GACAACACCG ATCACAGACC AACAAGTAAG 
CCAACAAAAA ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_009196.1
CDS	244..2178
Translation	MEETMKLATMEDTVEYCLFLIPDESRDSDKHKEILQKYIERIITRFAPMLVPYIWQNQPFNLKYKPGKGGVPAHMFGVTKFGDNIEDEWFIVYVIKQITKEFPELVARIEDNDGEFLLIEAADFLPKWLDPENSTNRVFFCHGELCIIPAPRKSGAESWLPTTPPTIPQALNIITAHSEKILASESIRAAVNRRIRGYPEKIQASLHRAHCFLPAGIVAVLKQRPRLVAAAVQAFYLRDPIDLRACRVFKTFLPETRIMTSVTFTKCLYAQLVQQRFVPDRRSGYRLPPPSDPQYRAHELGMKLAHGFEILCSKCSPHFSDCKKSLVTASPLWASFLESLKKNDYFKGLIEGSAQYRERLEMAENYFQLSVDWPESSLAMSPGEEILTLLQTIPFDIEDLKKEAANLPPEDDDQWLDLSPDQLDQLLQEAVGKKESESVSKEEKEQNYDLTEVSESMKAFISKVSTHKGAELPREPSEAPITFDADSFLNYFDKILGPRPNESDSDDLDDEDFECLDSDDDLDFETHEPGEEASLKGTLDNLKSYMAQMDQELAHTCISKSFTTRNQVEPVSQTTDNNSDEEDSGTGESVMAPVDVDLNLVSNILESYSSQAGLAGPASNLLQSMGVQLPDNTDHRPTSKPTKN

Target ORF information:

RefSeq Version	NM_007265.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ecdysoneless cell cycle regulator (ECD), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007265.2

ORF Insert Sequence:

ATGGAAGAAA CCATGAAGCT TGCTACGATG GAAGACACAG TGGAGTACTG CCTGTTCCTG 
ATACCAGATG AGTCAAGGGA CTCAGATAAA CATAAAGAGA TTCTTCAGAA GTACATTGAG 
AGAATAATCA CTCGGTTTGC ACCTATGCTG GTCCCCTACA TCTGGCAGAA TCAGCCTTTC 
AATCTTAAAT ATAAACCTGG GAAAGGAGGT GTTCCTGCTC ATATGTTTGG CGTGACAAAG 
TTTGGGGATA ACATTGAGGA TGAATGGTTT ATTGTTTATG TAATAAAGCA GATCACAAAG 
GAATTTCCAG AGTTAGTAGC AAGGATTGAA GACAATGATG GTGAATTCTT GTTAATAGAA 
GCTGCTGACT TTCTCCCTAA ATGGCTGGAT CCTGAAAATA GCACCAATAG GGTATTTTTC 
TGCCATGGGG AATTGTGTAT TATCCCTGCA CCAAGAAAAT CTGGAGCAGA ATCTTGGTTA 
CCCACCACAC CCCCAACAAT TCCACAAGCA TTGAATATAA TCACAGCACA TTCAGAAAAA 
ATACTTGCTT CAGAATCTAT ACGAGCTGCT GTGAATAGGC GCATCAGAGG GTACCCAGAA 
AAAATTCAGG CCTCACTTCA TCGAGCACAC TGCTTCCTTC CAGCTGGCAT TGTGGCAGTG 
CTAAAGCAGC GCCCCAGATT GGTGGCTGCA GCAGTCCAGG CATTTTACCT ACGAGACCCT 
ATTGACCTGC GAGCTTGTCG TGTTTTCAAG ACATTCTTGC CTGAAACACG AATAATGACA 
TCGGTCACAT TCACTAAATG TCTATATGCA CAATTGGTGC AACAAAGGTT TGTGCCAGAC 
CGGCGGAGTG GATACAGGCT GCCTCCTCCA TCTGATCCCC AGTACCGAGC CCATGAATTG 
GGCATGAAAT TGGCTCATGG ATTTGAGATC TTATGCTCCA AATGTAGCCC ACATTTTTCT 
GACTGCAAGA AATCCCTTGT GACTGCCTCA CCACTCTGGG CCAGTTTCCT TGAAAGTCTG 
AAAAAGAATG ATTACTTTAA GGGACTGATA GAAGGTTCTG CTCAGTACCG GGAAAGGCTA 
GAAATGGCAG AGAATTACTT CCAGCTCTCA GTAGACTGGC CAGAAAGTTC TCTTGCTATG 
AGCCCTGGTG AAGAAATCTT AACCTTATTA CAGACAATAC CATTTGATAT AGAAGACCTT 
AAGAAAGAAG CAGCTAATCT TCCCCCAGAG GATGATGACC AGTGGTTAGA TCTCTCACCA 
GATCAGCTGG ACCAGCTGCT GCAGGAAGCT GTTGGCAAAA AAGAATCCGA GTCTGTTTCC 
AAGGAGGAGA AGGAGCAGAA CTATGACTTA ACTGAAGTCT CAGAGAGCAT GAAAGCTTTC 
ATATCCAAAG TCTCAACCCA CAAGGGAGCA GAGCTGCCTC GAGAACCTTC TGAGGCTCCA 
ATCACTTTTG ATGCAGATTC TTTTCTTAAT TATTTTGATA AGATTTTAGG GCCAAGGCCT 
AATGAGTCAG ATTCTGATGA TCTGGATGAT GAAGACTTTG AATGTTTAGA TAGTGATGAT 
GACTTGGACT TTGAAACACA CGAACCTGGC GAAGAGGCTT CCCTGAAAGG AACACTTGAT 
AATCTCAAGT CATACATGGC CCAGATGGAC CAGGAACTAG CACACACCTG CATCAGCAAA 
AGTTTCACCA CTAGGAACCA AGTGGAACCT GTATCCCAGA CTACCGATAA CAATTCAGAT 
GAGGAAGATT CTGGTACGGG AGAATCTGTT ATGGCACCAG TAGATGTAGA CCTGAACCTG 
GTTTCAAATA TATTGGAATC CTATAGCTCC CAAGCTGGAC TGGCAGGACC TGCTTCCAAT 
CTTTTACAAA GCATGGGAGT GCAGCTGCCT GACAACACCG ATCACAGACC AACAAGTAAG 
CCAACAAAAA ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ECD ( NM_007265.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_009196.1 Homo sapiens ecdysoneless cell cycle regulator (ECD), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu05925
Clone ID Related Accession (Same CDS sequence)	NM_007265.2 , NM_007265.3
Accession Version	NM_007265.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1935bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	protein ecdysoneless homolog isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from D88208.1, BC103720.1 and AC016394.13. On May 17, 2019 this sequence version replaced NM_007265.2. Transcript Variant: This variant (1) represents a more abundant transcript and encodes isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR7346977.1338526.1, SRR1660803.56480.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000372979.9/ ENSP00000362070.4 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END##

ATGGAAGAAA CCATGAAGCT TGCTACGATG GAAGACACAG TGGAGTACTG CCTGTTCCTG 
ATACCAGATG AGTCAAGGGA CTCAGATAAA CATAAAGAGA TTCTTCAGAA GTACATTGAG 
AGAATAATCA CTCGGTTTGC ACCTATGCTG GTCCCCTACA TCTGGCAGAA TCAGCCTTTC 
AATCTTAAAT ATAAACCTGG GAAAGGAGGT GTTCCTGCTC ATATGTTTGG CGTGACAAAG 
TTTGGGGATA ACATTGAGGA TGAATGGTTT ATTGTTTATG TAATAAAGCA GATCACAAAG 
GAATTTCCAG AGTTAGTAGC AAGGATTGAA GACAATGATG GTGAATTCTT GTTAATAGAA 
GCTGCTGACT TTCTCCCTAA ATGGCTGGAT CCTGAAAATA GCACCAATAG GGTATTTTTC 
TGCCATGGGG AATTGTGTAT TATCCCTGCA CCAAGAAAAT CTGGAGCAGA ATCTTGGTTA 
CCCACCACAC CCCCAACAAT TCCACAAGCA TTGAATATAA TCACAGCACA TTCAGAAAAA 
ATACTTGCTT CAGAATCTAT ACGAGCTGCT GTGAATAGGC GCATCAGAGG GTACCCAGAA 
AAAATTCAGG CCTCACTTCA TCGAGCACAC TGCTTCCTTC CAGCTGGCAT TGTGGCAGTG 
CTAAAGCAGC GCCCCAGATT GGTGGCTGCA GCAGTCCAGG CATTTTACCT ACGAGACCCT 
ATTGACCTGC GAGCTTGTCG TGTTTTCAAG ACATTCTTGC CTGAAACACG AATAATGACA 
TCGGTCACAT TCACTAAATG TCTATATGCA CAATTGGTGC AACAAAGGTT TGTGCCAGAC 
CGGCGGAGTG GATACAGGCT GCCTCCTCCA TCTGATCCCC AGTACCGAGC CCATGAATTG 
GGCATGAAAT TGGCTCATGG ATTTGAGATC TTATGCTCCA AATGTAGCCC ACATTTTTCT 
GACTGCAAGA AATCCCTTGT GACTGCCTCA CCACTCTGGG CCAGTTTCCT TGAAAGTCTG 
AAAAAGAATG ATTACTTTAA GGGACTGATA GAAGGTTCTG CTCAGTACCG GGAAAGGCTA 
GAAATGGCAG AGAATTACTT CCAGCTCTCA GTAGACTGGC CAGAAAGTTC TCTTGCTATG 
AGCCCTGGTG AAGAAATCTT AACCTTATTA CAGACAATAC CATTTGATAT AGAAGACCTT 
AAGAAAGAAG CAGCTAATCT TCCCCCAGAG GATGATGACC AGTGGTTAGA TCTCTCACCA 
GATCAGCTGG ACCAGCTGCT GCAGGAAGCT GTTGGCAAAA AAGAATCCGA GTCTGTTTCC 
AAGGAGGAGA AGGAGCAGAA CTATGACTTA ACTGAAGTCT CAGAGAGCAT GAAAGCTTTC 
ATATCCAAAG TCTCAACCCA CAAGGGAGCA GAGCTGCCTC GAGAACCTTC TGAGGCTCCA 
ATCACTTTTG ATGCAGATTC TTTTCTTAAT TATTTTGATA AGATTTTAGG GCCAAGGCCT 
AATGAGTCAG ATTCTGATGA TCTGGATGAT GAAGACTTTG AATGTTTAGA TAGTGATGAT 
GACTTGGACT TTGAAACACA CGAACCTGGC GAAGAGGCTT CCCTGAAAGG AACACTTGAT 
AATCTCAAGT CATACATGGC CCAGATGGAC CAGGAACTAG CACACACCTG CATCAGCAAA 
AGTTTCACCA CTAGGAACCA AGTGGAACCT GTATCCCAGA CTACCGATAA CAATTCAGAT 
GAGGAAGATT CTGGTACGGG AGAATCTGTT ATGGCACCAG TAGATGTAGA CCTGAACCTG 
GTTTCAAATA TATTGGAATC CTATAGCTCC CAAGCTGGAC TGGCAGGACC TGCTTCCAAT 
CTTTTACAAA GCATGGGAGT GCAGCTGCCT GACAACACCG ATCACAGACC AACAAGTAAG 
CCAACAAAAA ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_009196.1
CDS	157..2091
Translation	MEETMKLATMEDTVEYCLFLIPDESRDSDKHKEILQKYIERIITRFAPMLVPYIWQNQPFNLKYKPGKGGVPAHMFGVTKFGDNIEDEWFIVYVIKQITKEFPELVARIEDNDGEFLLIEAADFLPKWLDPENSTNRVFFCHGELCIIPAPRKSGAESWLPTTPPTIPQALNIITAHSEKILASESIRAAVNRRIRGYPEKIQASLHRAHCFLPAGIVAVLKQRPRLVAAAVQAFYLRDPIDLRACRVFKTFLPETRIMTSVTFTKCLYAQLVQQRFVPDRRSGYRLPPPSDPQYRAHELGMKLAHGFEILCSKCSPHFSDCKKSLVTASPLWASFLESLKKNDYFKGLIEGSAQYRERLEMAENYFQLSVDWPESSLAMSPGEEILTLLQTIPFDIEDLKKEAANLPPEDDDQWLDLSPDQLDQLLQEAVGKKESESVSKEEKEQNYDLTEVSESMKAFISKVSTHKGAELPREPSEAPITFDADSFLNYFDKILGPRPNESDSDDLDDEDFECLDSDDDLDFETHEPGEEASLKGTLDNLKSYMAQMDQELAHTCISKSFTTRNQVEPVSQTTDNNSDEEDSGTGESVMAPVDVDLNLVSNILESYSSQAGLAGPASNLLQSMGVQLPDNTDHRPTSKPTKN

Target ORF information:

RefSeq Version	NM_007265.3
Organism	Homo sapiens(human)
Definition	Homo sapiens ecdysoneless cell cycle regulator (ECD), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007265.3

ORF Insert Sequence:

ATGGAAGAAA CCATGAAGCT TGCTACGATG GAAGACACAG TGGAGTACTG CCTGTTCCTG 
ATACCAGATG AGTCAAGGGA CTCAGATAAA CATAAAGAGA TTCTTCAGAA GTACATTGAG 
AGAATAATCA CTCGGTTTGC ACCTATGCTG GTCCCCTACA TCTGGCAGAA TCAGCCTTTC 
AATCTTAAAT ATAAACCTGG GAAAGGAGGT GTTCCTGCTC ATATGTTTGG CGTGACAAAG 
TTTGGGGATA ACATTGAGGA TGAATGGTTT ATTGTTTATG TAATAAAGCA GATCACAAAG 
GAATTTCCAG AGTTAGTAGC AAGGATTGAA GACAATGATG GTGAATTCTT GTTAATAGAA 
GCTGCTGACT TTCTCCCTAA ATGGCTGGAT CCTGAAAATA GCACCAATAG GGTATTTTTC 
TGCCATGGGG AATTGTGTAT TATCCCTGCA CCAAGAAAAT CTGGAGCAGA ATCTTGGTTA 
CCCACCACAC CCCCAACAAT TCCACAAGCA TTGAATATAA TCACAGCACA TTCAGAAAAA 
ATACTTGCTT CAGAATCTAT ACGAGCTGCT GTGAATAGGC GCATCAGAGG GTACCCAGAA 
AAAATTCAGG CCTCACTTCA TCGAGCACAC TGCTTCCTTC CAGCTGGCAT TGTGGCAGTG 
CTAAAGCAGC GCCCCAGATT GGTGGCTGCA GCAGTCCAGG CATTTTACCT ACGAGACCCT 
ATTGACCTGC GAGCTTGTCG TGTTTTCAAG ACATTCTTGC CTGAAACACG AATAATGACA 
TCGGTCACAT TCACTAAATG TCTATATGCA CAATTGGTGC AACAAAGGTT TGTGCCAGAC 
CGGCGGAGTG GATACAGGCT GCCTCCTCCA TCTGATCCCC AGTACCGAGC CCATGAATTG 
GGCATGAAAT TGGCTCATGG ATTTGAGATC TTATGCTCCA AATGTAGCCC ACATTTTTCT 
GACTGCAAGA AATCCCTTGT GACTGCCTCA CCACTCTGGG CCAGTTTCCT TGAAAGTCTG 
AAAAAGAATG ATTACTTTAA GGGACTGATA GAAGGTTCTG CTCAGTACCG GGAAAGGCTA 
GAAATGGCAG AGAATTACTT CCAGCTCTCA GTAGACTGGC CAGAAAGTTC TCTTGCTATG 
AGCCCTGGTG AAGAAATCTT AACCTTATTA CAGACAATAC CATTTGATAT AGAAGACCTT 
AAGAAAGAAG CAGCTAATCT TCCCCCAGAG GATGATGACC AGTGGTTAGA TCTCTCACCA 
GATCAGCTGG ACCAGCTGCT GCAGGAAGCT GTTGGCAAAA AAGAATCCGA GTCTGTTTCC 
AAGGAGGAGA AGGAGCAGAA CTATGACTTA ACTGAAGTCT CAGAGAGCAT GAAAGCTTTC 
ATATCCAAAG TCTCAACCCA CAAGGGAGCA GAGCTGCCTC GAGAACCTTC TGAGGCTCCA 
ATCACTTTTG ATGCAGATTC TTTTCTTAAT TATTTTGATA AGATTTTAGG GCCAAGGCCT 
AATGAGTCAG ATTCTGATGA TCTGGATGAT GAAGACTTTG AATGTTTAGA TAGTGATGAT 
GACTTGGACT TTGAAACACA CGAACCTGGC GAAGAGGCTT CCCTGAAAGG AACACTTGAT 
AATCTCAAGT CATACATGGC CCAGATGGAC CAGGAACTAG CACACACCTG CATCAGCAAA 
AGTTTCACCA CTAGGAACCA AGTGGAACCT GTATCCCAGA CTACCGATAA CAATTCAGAT 
GAGGAAGATT CTGGTACGGG AGAATCTGTT ATGGCACCAG TAGATGTAGA CCTGAACCTG 
GTTTCAAATA TATTGGAATC CTATAGCTCC CAAGCTGGAC TGGCAGGACC TGCTTCCAAT 
CTTTTACAAA GCATGGGAGT GCAGCTGCCT GACAACACCG ATCACAGACC AACAAGTAAG 
CCAACAAAAA ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ECD ( NM_001135752.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001129224.1 Homo sapiens ecdysoneless cell cycle regulator (ECD), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu24432
Clone ID Related Accession (Same CDS sequence)	NM_001135752.1
Accession Version	NM_001135752.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2034bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Homo sapiens(human)
Product	protein ecdysoneless homolog isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BM763002.1, AK225519.1, AK315711.1 and BC103720.1. Transcript Variant: This variant (2) omits an in-frame coding exon compared to variant 1 resulting in a shorter protein isoform (2). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK315711.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03267756 [ECO:0000350] ##Evidence-Data-END##

ATGGAAGAAA CCATGAAGCT TGCTACGATG GAAGACACAG TGGAGTACTG CCTGTTCCTG 
ATACCAGATG AGTCAAGGGA CTCAGATAAA CATAAAGAGA TTCTTCAGAA GTACATTGAG 
AGAATAATCA CTCGGTTTGC ACCTATGCTG GTCCCCTACA TCTGGCAGAA TCAGCCTTTC 
AATCTTAAAT ATAAACCTGG GAAAGGAGGT GTTCCTGCTC ATATGTTTGG CGTGACAAAG 
TTTGGGGATA ACATTGAGGA TGAATGGTTT ATTGTTTATG TAATAAAGCA GATCACAAAG 
GAATTTCCAG AGTTAGTAGC AAGGATTGAA GACAATGATG GTGAATTCTT GTTAATAGAA 
GCTGCTGACT TTCTCCCTAA ATGGCTGGAT CCTGAAAATA GCACCAATAG GGTATTTTTC 
TGCCATGGGG AATTGTGTAT TATCCCTGCA CCAAGAAAAT CTGGAGCAGA ATCTTGGTTA 
CCCACCACAC CCCCAACAAT TCCACAAGCA TTGAATATAA TCACAGCACA TTCAGAAAAA 
ATACTTGCTT CAGAATCTAT ACGAGCTGCT GTGAATAGGC GCATCAGAGG GTACCCAGAA 
AAAATTCAGG CCTCACTTCA TCGAGCACAC TGCTTCCTTC CAGCTGGCAT TGTGGCAGTG 
CTAAAGCAGC GCCCCAGATT GGTGGCTGCA GCAGTCCAGG CATTTTACCT ACGAGACCCT 
ATTGACCTGC GAGCTTGTCG TGTTTTCAAG ACATTCTTGC CTGAAACACG AATAATGACA 
TCGGTCACAT TCACTAAATG TCTATATGCA CAATTGGTGC AACAAAGGTT TGTGCCAGAC 
CGGCGGAGTG GATACAGGCT GCCTCCTCCA TCTGATCCCC AGTACCGAGC CCATGAATTG 
GGCATGAAAT TGGCTCATGG ATTTGAGATC TTATGCTCCA AATGTAGCCC ACATTTTTCT 
GACTGCAAGA AATCCCTTGT GACTGCCTCA CCACTCTGGG CCAGTTTCCT TGAAAGTCTG 
AAAAAGAATG ATTACTTTAA GGGACTGATA GAAGGTTCTG CTCAGTACCG GGAAAGGCTA 
GAAATGGCAG AGAATTACTT CCAGCTCTCA GTAGACTGGC CAGAAAGGTT GGAGGTGCAG 
TGGCGCGATC CTGGCTTACT GCAAGCTCCA CCTCCTGGGT TCACGCCATT CATCTGCCTC 
AGCCTCCTGA GTACCTGGGA CAACAGTTCT CTTGCTATGA GCCCTGGTGA AGAAATCTTA 
ACCTTATTAC AGACAATACC ATTTGATATA GAAGACCTTA AGAAAGAAGC AGCTAATCTT 
CCCCCAGAGG ATGATGACCA GTGGTTAGAT CTCTCACCAG ATCAGCTGGA CCAGCTGCTG 
CAGGAAGCTG TTGGCAAAAA AGAATCCGAG TCTGTTTCCA AGGAGGAGAA GGAGCAGAAC 
TATGACTTAA CTGAAGTCTC AGAGAGCATG AAAGCTTTCA TATCCAAAGT CTCAACCCAC 
AAGGGAGCAG AGCTGCCTCG AGAACCTTCT GAGGCTCCAA TCACTTTTGA TGCAGATTCT 
TTTCTTAATT ATTTTGATAA GATTTTAGGG CCAAGGCCTA ATGAGTCAGA TTCTGATGAT 
CTGGATGATG AAGACTTTGA ATGTTTAGAT AGTGATGATG ACTTGGACTT TGAAACACAC 
GAACCTGGCG AAGAGGCTTC CCTGAAAGGA ACACTTGATA ATCTCAAGTC ATACATGGCC 
CAGATGGACC AGGAACTAGC ACACACCTGC ATCAGCAAAA GTTTCACCAC TAGGAACCAA 
GTGGAACCTG TATCCCAGAC TACCGATAAC AATTCAGATG AGGAAGATTC TGGTACGGGA 
GAATCTGTTA TGGCACCAGT AGATGTAGAC CTGAACCTGG TTTCAAATAT ATTGGAATCC 
TATAGCTCCC AAGCTGGACT GGCAGGACCT GCTTCCAATC TTTTACAAAG CATGGGAGTG 
CAGCTGCCTG ACAACACCGA TCACAGACCA ACAAGTAAGC CAACAAAAAA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001129224.1
CDS	244..2277
Translation	MEETMKLATMEDTVEYCLFLIPDESRDSDKHKEILQKYIERIITRFAPMLVPYIWQNQPFNLKYKPGKGGVPAHMFGVTKFGDNIEDEWFIVYVIKQITKEFPELVARIEDNDGEFLLIEAADFLPKWLDPENSTNRVFFCHGELCIIPAPRKSGAESWLPTTPPTIPQALNIITAHSEKILASESIRAAVNRRIRGYPEKIQASLHRAHCFLPAGIVAVLKQRPRLVAAAVQAFYLRDPIDLRACRVFKTFLPETRIMTSVTFTKCLYAQLVQQRFVPDRRSGYRLPPPSDPQYRAHELGMKLAHGFEILCSKCSPHFSDCKKSLVTASPLWASFLESLKKNDYFKGLIEGSAQYRERLEMAENYFQLSVDWPERLEVQWRDPGLLQAPPPGFTPFICLSLLSTWDNSSLAMSPGEEILTLLQTIPFDIEDLKKEAANLPPEDDDQWLDLSPDQLDQLLQEAVGKKESESVSKEEKEQNYDLTEVSESMKAFISKVSTHKGAELPREPSEAPITFDADSFLNYFDKILGPRPNESDSDDLDDEDFECLDSDDDLDFETHEPGEEASLKGTLDNLKSYMAQMDQELAHTCISKSFTTRNQVEPVSQTTDNNSDEEDSGTGESVMAPVDVDLNLVSNILESYSSQAGLAGPASNLLQSMGVQLPDNTDHRPTSKPTKN

Target ORF information:

RefSeq Version	NM_001135752.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ecdysoneless cell cycle regulator (ECD), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001135752.1

ORF Insert Sequence:

ATGGAAGAAA CCATGAAGCT TGCTACGATG GAAGACACAG TGGAGTACTG CCTGTTCCTG 
ATACCAGATG AGTCAAGGGA CTCAGATAAA CATAAAGAGA TTCTTCAGAA GTACATTGAG 
AGAATAATCA CTCGGTTTGC ACCTATGCTG GTCCCCTACA TCTGGCAGAA TCAGCCTTTC 
AATCTTAAAT ATAAACCTGG GAAAGGAGGT GTTCCTGCTC ATATGTTTGG CGTGACAAAG 
TTTGGGGATA ACATTGAGGA TGAATGGTTT ATTGTTTATG TAATAAAGCA GATCACAAAG 
GAATTTCCAG AGTTAGTAGC AAGGATTGAA GACAATGATG GTGAATTCTT GTTAATAGAA 
GCTGCTGACT TTCTCCCTAA ATGGCTGGAT CCTGAAAATA GCACCAATAG GGTATTTTTC 
TGCCATGGGG AATTGTGTAT TATCCCTGCA CCAAGAAAAT CTGGAGCAGA ATCTTGGTTA 
CCCACCACAC CCCCAACAAT TCCACAAGCA TTGAATATAA TCACAGCACA TTCAGAAAAA 
ATACTTGCTT CAGAATCTAT ACGAGCTGCT GTGAATAGGC GCATCAGAGG GTACCCAGAA 
AAAATTCAGG CCTCACTTCA TCGAGCACAC TGCTTCCTTC CAGCTGGCAT TGTGGCAGTG 
CTAAAGCAGC GCCCCAGATT GGTGGCTGCA GCAGTCCAGG CATTTTACCT ACGAGACCCT 
ATTGACCTGC GAGCTTGTCG TGTTTTCAAG ACATTCTTGC CTGAAACACG AATAATGACA 
TCGGTCACAT TCACTAAATG TCTATATGCA CAATTGGTGC AACAAAGGTT TGTGCCAGAC 
CGGCGGAGTG GATACAGGCT GCCTCCTCCA TCTGATCCCC AGTACCGAGC CCATGAATTG 
GGCATGAAAT TGGCTCATGG ATTTGAGATC TTATGCTCCA AATGTAGCCC ACATTTTTCT 
GACTGCAAGA AATCCCTTGT GACTGCCTCA CCACTCTGGG CCAGTTTCCT TGAAAGTCTG 
AAAAAGAATG ATTACTTTAA GGGACTGATA GAAGGTTCTG CTCAGTACCG GGAAAGGCTA 
GAAATGGCAG AGAATTACTT CCAGCTCTCA GTAGACTGGC CAGAAAGGTT GGAGGTGCAG 
TGGCGCGATC CTGGCTTACT GCAAGCTCCA CCTCCTGGGT TCACGCCATT CATCTGCCTC 
AGCCTCCTGA GTACCTGGGA CAACAGTTCT CTTGCTATGA GCCCTGGTGA AGAAATCTTA 
ACCTTATTAC AGACAATACC ATTTGATATA GAAGACCTTA AGAAAGAAGC AGCTAATCTT 
CCCCCAGAGG ATGATGACCA GTGGTTAGAT CTCTCACCAG ATCAGCTGGA CCAGCTGCTG 
CAGGAAGCTG TTGGCAAAAA AGAATCCGAG TCTGTTTCCA AGGAGGAGAA GGAGCAGAAC 
TATGACTTAA CTGAAGTCTC AGAGAGCATG AAAGCTTTCA TATCCAAAGT CTCAACCCAC 
AAGGGAGCAG AGCTGCCTCG AGAACCTTCT GAGGCTCCAA TCACTTTTGA TGCAGATTCT 
TTTCTTAATT ATTTTGATAA GATTTTAGGG CCAAGGCCTA ATGAGTCAGA TTCTGATGAT 
CTGGATGATG AAGACTTTGA ATGTTTAGAT AGTGATGATG ACTTGGACTT TGAAACACAC 
GAACCTGGCG AAGAGGCTTC CCTGAAAGGA ACACTTGATA ATCTCAAGTC ATACATGGCC 
CAGATGGACC AGGAACTAGC ACACACCTGC ATCAGCAAAA GTTTCACCAC TAGGAACCAA 
GTGGAACCTG TATCCCAGAC TACCGATAAC AATTCAGATG AGGAAGATTC TGGTACGGGA 
GAATCTGTTA TGGCACCAGT AGATGTAGAC CTGAACCTGG TTTCAAATAT ATTGGAATCC 
TATAGCTCCC AAGCTGGACT GGCAGGACCT GCTTCCAATC TTTTACAAAG CATGGGAGTG 
CAGCTGCCTG ACAACACCGA TCACAGACCA ACAAGTAAGC CAACAAAAAA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ECD ( NM_001135753.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001129225.1 Homo sapiens ecdysoneless cell cycle regulator (ECD), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu24433
Clone ID Related Accession (Same CDS sequence)	NM_001135753.1
Accession Version	NM_001135753.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1806bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Homo sapiens(human)
Product	protein ecdysoneless homolog isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BM763002.1, AK225519.1, BC000721.2 and BC103720.1. Transcript Variant: This variant includes an additional in-frame coding exon compared to variant 1 resulting in a longer protein isoform (3). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK225519.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGGAAGAAA CCATGAAGCT TGCTACGATG GAAGACACAG TGGAGTACTG CCTGTTCCTG 
ATACCAGATG AGTCAAGGGA CTCAGATAAA CATAAAGAGA TTCTTCAGAA GTACATTGAG 
AGAATAATCA CTCGGTTTGC ACCTATGCTG GTCCCCTACA TCTGGCAGAA TCAGCCTTTC 
AATCTTAAAT ATAAACCTGG GAAAGGAGGT GTTCCTGCTC ATATGTTTGG CGTGACAAAG 
TTTGGGGATA ACATTGAGGA TGAATGGTTT ATTGTTTATG TAATAAAGCA GATCACAAAG 
GAATTTCCAG AGTTAGTAGC AAGGATTGAA GACAATGATG GTGAATTCTT GTTAATAGAA 
GCTGCTGACT TTCTCCCTAA ATGGCTGGAT CCTGAAAATA GCACCAATAG GGTATTTTTC 
TGCCATGGGG AATTGTGTAT TATCCCTGCA CCAAGAAAAT CTGGAGCAGA ATCTTGGTTA 
CCCACCACAC CCCCAACAAT TCCACAAGCA TTGAATATAA TCACAGCACA TTCAGAAAAA 
ATACTTGCTT CAGAATCTAT ACGAGCTGCT GTGAATAGGC GCATCAGAGG GTACCCAGAA 
AAAATTCAGG CCTCACTTCA TCGAGCACAC TGCTTCCTTC CAGCTGGCAT TGTGGCAGTG 
CTAAAGCAGC GCCCCAGATT GGTGGCTGCA GCAGTCCAGG CATTTTACCT ACGAGACCCT 
ATTGACCTGC GAGCTTGTCG TGTTTTCAAG ACATTCTTGC CTGAAACACG AATAATGACA 
TCGGCTCATG GATTTGAGAT CTTATGCTCC AAATGTAGCC CACATTTTTC TGACTGCAAG 
AAATCCCTTG TGACTGCCTC ACCACTCTGG GCCAGTTTCC TTGAAAGTCT GAAAAAGAAT 
GATTACTTTA AGGGACTGAT AGAAGGTTCT GCTCAGTACC GGGAAAGGCT AGAAATGGCA 
GAGAATTACT TCCAGCTCTC AGTAGACTGG CCAGAAAGTT CTCTTGCTAT GAGCCCTGGT 
GAAGAAATCT TAACCTTATT ACAGACAATA CCATTTGATA TAGAAGACCT TAAGAAAGAA 
GCAGCTAATC TTCCCCCAGA GGATGATGAC CAGTGGTTAG ATCTCTCACC AGATCAGCTG 
GACCAGCTGC TGCAGGAAGC TGTTGGCAAA AAAGAATCCG AGTCTGTTTC CAAGGAGGAG 
AAGGAGCAGA ACTATGACTT AACTGAAGTC TCAGAGAGCA TGAAAGCTTT CATATCCAAA 
GTCTCAACCC ACAAGGGAGC AGAGCTGCCT CGAGAACCTT CTGAGGCTCC AATCACTTTT 
GATGCAGATT CTTTTCTTAA TTATTTTGAT AAGATTTTAG GGCCAAGGCC TAATGAGTCA 
GATTCTGATG ATCTGGATGA TGAAGACTTT GAATGTTTAG ATAGTGATGA TGACTTGGAC 
TTTGAAACAC ACGAACCTGG CGAAGAGGCT TCCCTGAAAG GAACACTTGA TAATCTCAAG 
TCATACATGG CCCAGATGGA CCAGGAACTA GCACACACCT GCATCAGCAA AAGTTTCACC 
ACTAGGAACC AAGTGGAACC TGTATCCCAG ACTACCGATA ACAATTCAGA TGAGGAAGAT 
TCTGGTACGG GAGAATCTGT TATGGCACCA GTAGATGTAG ACCTGAACCT GGTTTCAAAT 
ATATTGGAAT CCTATAGCTC CCAAGCTGGA CTGGCAGGAC CTGCTTCCAA TCTTTTACAA 
AGCATGGGAG TGCAGCTGCC TGACAACACC GATCACAGAC CAACAAGTAA GCCAACAAAA 
AATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001129225.1
CDS	244..2049
Translation	MEETMKLATMEDTVEYCLFLIPDESRDSDKHKEILQKYIERIITRFAPMLVPYIWQNQPFNLKYKPGKGGVPAHMFGVTKFGDNIEDEWFIVYVIKQITKEFPELVARIEDNDGEFLLIEAADFLPKWLDPENSTNRVFFCHGELCIIPAPRKSGAESWLPTTPPTIPQALNIITAHSEKILASESIRAAVNRRIRGYPEKIQASLHRAHCFLPAGIVAVLKQRPRLVAAAVQAFYLRDPIDLRACRVFKTFLPETRIMTSAHGFEILCSKCSPHFSDCKKSLVTASPLWASFLESLKKNDYFKGLIEGSAQYRERLEMAENYFQLSVDWPESSLAMSPGEEILTLLQTIPFDIEDLKKEAANLPPEDDDQWLDLSPDQLDQLLQEAVGKKESESVSKEEKEQNYDLTEVSESMKAFISKVSTHKGAELPREPSEAPITFDADSFLNYFDKILGPRPNESDSDDLDDEDFECLDSDDDLDFETHEPGEEASLKGTLDNLKSYMAQMDQELAHTCISKSFTTRNQVEPVSQTTDNNSDEEDSGTGESVMAPVDVDLNLVSNILESYSSQAGLAGPASNLLQSMGVQLPDNTDHRPTSKPTKN

Target ORF information:

RefSeq Version	NM_001135753.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ecdysoneless cell cycle regulator (ECD), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001135753.1

ORF Insert Sequence:

ATGGAAGAAA CCATGAAGCT TGCTACGATG GAAGACACAG TGGAGTACTG CCTGTTCCTG 
ATACCAGATG AGTCAAGGGA CTCAGATAAA CATAAAGAGA TTCTTCAGAA GTACATTGAG 
AGAATAATCA CTCGGTTTGC ACCTATGCTG GTCCCCTACA TCTGGCAGAA TCAGCCTTTC 
AATCTTAAAT ATAAACCTGG GAAAGGAGGT GTTCCTGCTC ATATGTTTGG CGTGACAAAG 
TTTGGGGATA ACATTGAGGA TGAATGGTTT ATTGTTTATG TAATAAAGCA GATCACAAAG 
GAATTTCCAG AGTTAGTAGC AAGGATTGAA GACAATGATG GTGAATTCTT GTTAATAGAA 
GCTGCTGACT TTCTCCCTAA ATGGCTGGAT CCTGAAAATA GCACCAATAG GGTATTTTTC 
TGCCATGGGG AATTGTGTAT TATCCCTGCA CCAAGAAAAT CTGGAGCAGA ATCTTGGTTA 
CCCACCACAC CCCCAACAAT TCCACAAGCA TTGAATATAA TCACAGCACA TTCAGAAAAA 
ATACTTGCTT CAGAATCTAT ACGAGCTGCT GTGAATAGGC GCATCAGAGG GTACCCAGAA 
AAAATTCAGG CCTCACTTCA TCGAGCACAC TGCTTCCTTC CAGCTGGCAT TGTGGCAGTG 
CTAAAGCAGC GCCCCAGATT GGTGGCTGCA GCAGTCCAGG CATTTTACCT ACGAGACCCT 
ATTGACCTGC GAGCTTGTCG TGTTTTCAAG ACATTCTTGC CTGAAACACG AATAATGACA 
TCGGCTCATG GATTTGAGAT CTTATGCTCC AAATGTAGCC CACATTTTTC TGACTGCAAG 
AAATCCCTTG TGACTGCCTC ACCACTCTGG GCCAGTTTCC TTGAAAGTCT GAAAAAGAAT 
GATTACTTTA AGGGACTGAT AGAAGGTTCT GCTCAGTACC GGGAAAGGCT AGAAATGGCA 
GAGAATTACT TCCAGCTCTC AGTAGACTGG CCAGAAAGTT CTCTTGCTAT GAGCCCTGGT 
GAAGAAATCT TAACCTTATT ACAGACAATA CCATTTGATA TAGAAGACCT TAAGAAAGAA 
GCAGCTAATC TTCCCCCAGA GGATGATGAC CAGTGGTTAG ATCTCTCACC AGATCAGCTG 
GACCAGCTGC TGCAGGAAGC TGTTGGCAAA AAAGAATCCG AGTCTGTTTC CAAGGAGGAG 
AAGGAGCAGA ACTATGACTT AACTGAAGTC TCAGAGAGCA TGAAAGCTTT CATATCCAAA 
GTCTCAACCC ACAAGGGAGC AGAGCTGCCT CGAGAACCTT CTGAGGCTCC AATCACTTTT 
GATGCAGATT CTTTTCTTAA TTATTTTGAT AAGATTTTAG GGCCAAGGCC TAATGAGTCA 
GATTCTGATG ATCTGGATGA TGAAGACTTT GAATGTTTAG ATAGTGATGA TGACTTGGAC 
TTTGAAACAC ACGAACCTGG CGAAGAGGCT TCCCTGAAAG GAACACTTGA TAATCTCAAG 
TCATACATGG CCCAGATGGA CCAGGAACTA GCACACACCT GCATCAGCAA AAGTTTCACC 
ACTAGGAACC AAGTGGAACC TGTATCCCAG ACTACCGATA ACAATTCAGA TGAGGAAGAT 
TCTGGTACGG GAGAATCTGT TATGGCACCA GTAGATGTAG ACCTGAACCT GGTTTCAAAT 
ATATTGGAAT CCTATAGCTC CCAAGCTGGA CTGGCAGGAC CTGCTTCCAA TCTTTTACAA 
AGCATGGGAG TGCAGCTGCC TGACAACACC GATCACAGAC CAACAAGTAA GCCAACAAAA 
AATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

A Novel Interaction of Ecdysoneless (ECD) Protein with R2TP Complex Component RUVBL1 Is Required for the Functional Role of ECD in Cell Cycle Progression.
Molecular and cellular biology36(6)886-99(2015 Dec)
Mir RA,Bele A,Mirza S,Srivastava S,Olou AA,Ammons SA,Kim JH,Gurumurthy CB,Qiu F,Band H,Band V

The cell cycle regulator ecdysoneless cooperates with H-Ras to promote oncogenic transformation of human mammary epithelial cells.
Cell cycle (Georgetown, Tex.)14(7)990-1000(2015)
Bele A,Mirza S,Zhang Y,Ahmad Mir R,Lin S,Kim JH,Gurumurthy CB,West W,Qiu F,Band H,Band V

Phosphorylation-dependent PIH1D1 interactions define substrate specificity of the R2TP cochaperone complex.
Cell reports7(1)19-26(2014 Apr)
Ho?ej?? Z,Stach L,Flower TG,Joshi D,Flynn H,Skehel JM,O'Reilly NJ,Ogrodowicz RW,Smerdon SJ,Boulton SJ

The Salmonella type III effector SspH2 specifically exploits the NLR co-chaperone activity of SGT1 to subvert immunity.
PLoS pathogens9(7)e1003518(2013)
Bhavsar AP,Brown NF,Stoepel J,Wiermer M,Martin DD,Hsu KJ,Imami K,Ross CJ,Hayden MR,Foster LJ,Li X,Hieter P,Finlay BB

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ECD cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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