CHST14 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	CHST14
Entrez Gene ID	113189
Full Name	carbohydrate sulfotransferase 14
Synonyms	ATCS,D4ST1,EDSMC1,HNK1ST
General protein information	Preferred Names carbohydrate sulfotransferase 14 Names carbohydrate sulfotransferase 14 carbohydrate (N-acetylgalactosamine 4-0) sulfotransferase 14 carbohydrate (dermatan 4) sulfotransferase 14 dermatan 4 sulfotransferase 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 15 Map Location: 15q15.1
Summary	This gene encodes a member of the HNK-1 family of sulfotransferases. The encoded protein transfers sulfate to the C-4 hydroxyl of N-acetylgalactosamine residues in dermatan sulfate. Mutations in this gene have been associated with adducted thumb-clubfoot syndrome.[provided by RefSeq, Mar 2010].
Disorder MIM:	608429
Disorder Html:	Ehlers-Danlos syndrome, musculocontractural type 1, 601776 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_130468.4	NP_569735.1	carbohydrate sulfotransferase 14
NM_130468.3	NP_569735.1	carbohydrate sulfotransferase 14

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	CHST14	XP_510310.2
	CHST14	XP_001096685.1
Xenopus tropicalis (tropical clawed frog)	chst14	XP_004917361.1
Homo sapiens (human)	CHST14	NP_569735.1
Canis lupus familiaris (dog)	CHST14	XP_005638734.1
Rattus norvegicus (Norway rat)	Chst14	NP_001103109.1
Mus musculus (house mouse)	Chst14	NP_082393.3
Gallus gallus (chicken)	CHST14	XP_003641334.2
Bos taurus (cattle)	CHST14	NP_001091481.1
Danio rerio (zebrafish)	chst14	NP_997994.1

Gene Ontology
Bibliography

Related articles in PubMed

Defect in dermatan sulfate in urine of patients with Ehlers-Danlos syndrome caused by a CHST14/D4ST1 deficiency.
Mizumoto S, Kosho T, Hatamochi A, Honda T, Yamaguchi T, Okamoto N, Miyake N, Yamada S, Sugahara K
Clinical biochemistry50(12)670-677(2017 Aug)

CHST14/D4ST1 deficiency: New form of Ehlers-Danlos syndrome.
Kosho T
Pediatrics international : official journal of the Japan Pediatric Society58(2)88-99(2016 Feb)

The phenotype of the musculocontractural type of Ehlers-Danlos syndrome due to CHST14 mutations.
Janecke AR, Li B, Boehm M, Krabichler B, Rohrbach M, M?ller T, Fuchs I, Golas G, Katagiri Y, Ziegler SG, Gahl WA, Wilnai Y, Zoppi N, Geller HM, Giunta C, Slavotinek A, Steinmann B
American journal of medical genetics. Part A170A(1)103-15(2016 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Dermatan sulfate does not appear in urine of patients with Ehlers-Danlos syndrome caused by a CHST14/D4ST1 deficiency.
Title: Defect in dermatan sulfate in urine of patients with Ehlers-Danlos syndrome caused by a CHST14/D4ST1 deficiency.

patients with CHST14/D4ST1 deficiency develop progressive multisystem fragility-related manifestations, establishment of a comprehensive and detailed natural history and health-care guidelines as well as further elucidation of the pathophysiology in view of future etiology-based therapy are crucial
Title: CHST14/D4ST1 deficiency: New form of Ehlers-Danlos syndrome.

CHST14 gene mutations are associated with musculocontractural type of Ehlers-Danlos syndrome.
Title: The phenotype of the musculocontractural type of Ehlers-Danlos syndrome due to CHST14 mutations.

We report on the detailed clinical characterization of two sisters with musculocontractural Ehlers-Danlos syndrome caused by a homozygous mutation in the CHST14 gene.
Title: Extracellular matrix and platelet function in patients with musculocontractural Ehlers-Danlos syndrome caused by mutations in the CHST14 gene.

Loss of dermatan-4-sulfotransferase 1 (D4ST1/CHST14) function represents the first dermatan sulfate biosynthesis defect, "dermatan sulfate-deficient adducted thumb-clubfoot syndrome".
Title: Loss of dermatan-4-sulfotransferase 1 (D4ST1/CHST14) function represents the first dermatan sulfate biosynthesis defect, "dermatan sulfate-deficient adducted thumb-clubfoot syndrome".

The following CHST14 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CHST14 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu18446	NM_130468.4 Latest version!	Homo sapiens carbohydrate sulfotransferase 14 (CHST14), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
View Old Accession Versions >>
OHu18446	NM_130468.3	Homo sapiens carbohydrate sulfotransferase 14 (CHST14), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CHST14 ( NM_130468.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_569735.1 Homo sapiens carbohydrate sulfotransferase 14 (CHST14), mRNA.

Price & Availability↑

CloneID	OHu18446
Clone ID Related Accession (Same CDS sequence)	NM_130468.4 , NM_130468.3
Accession Version	NM_130468.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1131bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-19
Organism	Homo sapiens(human)
Product	carbohydrate sulfotransferase 14
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CR997569.1 and AB066595.1. On Nov 22, 2019 this sequence version replaced NM_130468.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript is intronless :: AB066595.1, BC053633.1 [ECO:0000345] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTTCCCCC GCCCGCTGAC CCCGCTGGCG GCCCCAAATG GCGCCGAGCC CCTGGGCCGG 
GCGCTGAGGC GGGCCCCTCT GGGCAGGGCC CGGGCGGGGC TGGGTGGGCC GCCCCTGCTG 
CTGCCGTCCA TGCTGATGTT TGCGGTGATC GTGGCCTCCA GCGGGCTGCT GCTCATGATC 
GAGCGGGGCA TCCTGGCCGA GATGAAGCCC CTGCCCCTGC ACCCGCCCGG CCGCGAGGGC 
ACAGCCTGGC GCGGGAAAGC CCCCAAGCCT GGGGGCCTGT CCCTCAGGGC TGGGGACGCG 
GACTTGCAAG TGCGGCAGGA CGTCCGGAAC AGGACCCTGC GGGCGGTGTG CGGACAGCCA 
GGCATGCCCC GGGACCCCTG GGACTTGCCG GTGGGGCAGC GGCGCACCCT GCTGCGCCAC 
ATCCTCGTAA GTGACCGTTA CCGCTTCCTC TACTGCTACG TCCCCAAGGT GGCCTGCTCT 
AACTGGAAGC GGGTGATGAA GGTGCTGGCA GGCGTCCTGG ACAGCGTGGA CGTCCGCCTC 
AAGATGGACC ACCGCAGTGA CCTGGTGTTC CTGGCCGACC TGCGGCCTGA GGAGATTCGC 
TACCGCCTGC AGCACTACTT TAAGTTCCTG TTTGTGCGGG AGCCCTTGGA ACGCCTCCTC 
TCTGCCTACC GCAACAAGTT TGGCGAGATC CGAGAGTACC AGCAACGCTA TGGGGCTGAG 
ATAGTGAGGC GGTACAGGGC TGGAGCGGGG CCCAGCCCTG CAGGCGACGA TGTCACATTC 
CCCGAGTTCC TGAGATACCT GGTGGATGAG GACCCTGAGC GCATGAATGA GCATTGGATG 
CCCGTGTACC ACCTGTGCCA GCCTTGTGCC GTGCACTATG ACTTTGTGGG CTCCTATGAG 
AGGCTGGAGG CTGATGCAAA TCAGGTGCTG GAGTGGGTAC GGGCACCACC TCACGTCCGA 
TTTCCAGCTC GCCAGGCCTG GTACCGGCCA GCCAGCCCCG AAAGCCTGCA TTACCACTTG 
TGCAGTGCCC CCCGGGCCCT GCTGCAGGAT GTGCTGCCTA AGTATATCCT GGACTTCTCC 
CTCTTTGCCT ACCCACTGCC TAATGTCACC AAGGAGGCGT GTCAGCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_569735.1
CDS	231..1361
Translation	MFPRPLTPLAAPNGAEPLGRALRRAPLGRARAGLGGPPLLLPSMLMFAVIVASSGLLLMIERGILAEMKPLPLHPPGREGTAWRGKAPKPGGLSLRAGDADLQVRQDVRNRTLRAVCGQPGMPRDPWDLPVGQRRTLLRHILVSDRYRFLYCYVPKVACSNWKRVMKVLAGVLDSVDVRLKMDHRSDLVFLADLRPEEIRYRLQHYFKFLFVREPLERLLSAYRNKFGEIREYQQRYGAEIVRRYRAGAGPSPAGDDVTFPEFLRYLVDEDPERMNEHWMPVYHLCQPCAVHYDFVGSYERLEADANQVLEWVRAPPHVRFPARQAWYRPASPESLHYHLCSAPRALLQDVLPKYILDFSLFAYPLPNVTKEACQQ

Target ORF information:

RefSeq Version	NM_130468.4
Organism	Homo sapiens(human)
Definition	Homo sapiens carbohydrate sulfotransferase 14 (CHST14), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_130468.4

ORF Insert Sequence:

ATGTTCCCCC GCCCGCTGAC CCCGCTGGCG GCCCCAAATG GCGCCGAGCC CCTGGGCCGG 
GCGCTGAGGC GGGCCCCTCT GGGCAGGGCC CGGGCGGGGC TGGGTGGGCC GCCCCTGCTG 
CTGCCGTCCA TGCTGATGTT TGCGGTGATC GTGGCCTCCA GCGGGCTGCT GCTCATGATC 
GAGCGGGGCA TCCTGGCCGA GATGAAGCCC CTGCCCCTGC ACCCGCCCGG CCGCGAGGGC 
ACAGCCTGGC GCGGGAAAGC CCCCAAGCCT GGGGGCCTGT CCCTCAGGGC TGGGGACGCG 
GACTTGCAAG TGCGGCAGGA CGTCCGGAAC AGGACCCTGC GGGCGGTGTG CGGACAGCCA 
GGCATGCCCC GGGACCCCTG GGACTTGCCG GTGGGGCAGC GGCGCACCCT GCTGCGCCAC 
ATCCTCGTAA GTGACCGTTA CCGCTTCCTC TACTGCTACG TCCCCAAGGT GGCCTGCTCT 
AACTGGAAGC GGGTGATGAA GGTGCTGGCA GGCGTCCTGG ACAGCGTGGA CGTCCGCCTC 
AAGATGGACC ACCGCAGTGA CCTGGTGTTC CTGGCCGACC TGCGGCCTGA GGAGATTCGC 
TACCGCCTGC AGCACTACTT TAAGTTCCTG TTTGTGCGGG AGCCCTTGGA ACGCCTCCTC 
TCTGCCTACC GCAACAAGTT TGGCGAGATC CGAGAGTACC AGCAACGCTA TGGGGCTGAG 
ATAGTGAGGC GGTACAGGGC TGGAGCGGGG CCCAGCCCTG CAGGCGACGA TGTCACATTC 
CCCGAGTTCC TGAGATACCT GGTGGATGAG GACCCTGAGC GCATGAATGA GCATTGGATG 
CCCGTGTACC ACCTGTGCCA GCCTTGTGCC GTGCACTATG ACTTTGTGGG CTCCTATGAG 
AGGCTGGAGG CTGATGCAAA TCAGGTGCTG GAGTGGGTAC GGGCACCACC TCACGTCCGA 
TTTCCAGCTC GCCAGGCCTG GTACCGGCCA GCCAGCCCCG AAAGCCTGCA TTACCACTTG 
TGCAGTGCCC CCCGGGCCCT GCTGCAGGAT GTGCTGCCTA AGTATATCCT GGACTTCTCC 
CTCTTTGCCT ACCCACTGCC TAATGTCACC AAGGAGGCGT GTCAGCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHST14 ( NM_130468.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_569735.1 Homo sapiens carbohydrate sulfotransferase 14 (CHST14), mRNA.

Price & Availability↑

CloneID	OHu18446
Clone ID Related Accession (Same CDS sequence)	NM_130468.4 , NM_130468.3
Accession Version	NM_130468.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1131bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-03
Organism	Homo sapiens(human)
Product	carbohydrate sulfotransferase 14
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CR997569.1 and AB066595.1. This sequence is a reference standard in the RefSeqGene project. On Mar 27, 2010 this sequence version replaced NM_130468.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTTCCCCC GCCCGCTGAC CCCGCTGGCG GCCCCAAATG GCGCCGAGCC CCTGGGCCGG 
GCGCTGAGGC GGGCCCCTCT GGGCAGGGCC CGGGCGGGGC TGGGTGGGCC GCCCCTGCTG 
CTGCCGTCCA TGCTGATGTT TGCGGTGATC GTGGCCTCCA GCGGGCTGCT GCTCATGATC 
GAGCGGGGCA TCCTGGCCGA GATGAAGCCC CTGCCCCTGC ACCCGCCCGG CCGCGAGGGC 
ACAGCCTGGC GCGGGAAAGC CCCCAAGCCT GGGGGCCTGT CCCTCAGGGC TGGGGACGCG 
GACTTGCAAG TGCGGCAGGA CGTCCGGAAC AGGACCCTGC GGGCGGTGTG CGGACAGCCA 
GGCATGCCCC GGGACCCCTG GGACTTGCCG GTGGGGCAGC GGCGCACCCT GCTGCGCCAC 
ATCCTCGTAA GTGACCGTTA CCGCTTCCTC TACTGCTACG TCCCCAAGGT GGCCTGCTCT 
AACTGGAAGC GGGTGATGAA GGTGCTGGCA GGCGTCCTGG ACAGCGTGGA CGTCCGCCTC 
AAGATGGACC ACCGCAGTGA CCTGGTGTTC CTGGCCGACC TGCGGCCTGA GGAGATTCGC 
TACCGCCTGC AGCACTACTT TAAGTTCCTG TTTGTGCGGG AGCCCTTGGA ACGCCTCCTC 
TCTGCCTACC GCAACAAGTT TGGCGAGATC CGAGAGTACC AGCAACGCTA TGGGGCTGAG 
ATAGTGAGGC GGTACAGGGC TGGAGCGGGG CCCAGCCCTG CAGGCGACGA TGTCACATTC 
CCCGAGTTCC TGAGATACCT GGTGGATGAG GACCCTGAGC GCATGAATGA GCATTGGATG 
CCCGTGTACC ACCTGTGCCA GCCTTGTGCC GTGCACTATG ACTTTGTGGG CTCCTATGAG 
AGGCTGGAGG CTGATGCAAA TCAGGTGCTG GAGTGGGTAC GGGCACCACC TCACGTCCGA 
TTTCCAGCTC GCCAGGCCTG GTACCGGCCA GCCAGCCCCG AAAGCCTGCA TTACCACTTG 
TGCAGTGCCC CCCGGGCCCT GCTGCAGGAT GTGCTGCCTA AGTATATCCT GGACTTCTCC 
CTCTTTGCCT ACCCACTGCC TAATGTCACC AAGGAGGCGT GTCAGCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_569735.1
CDS	254..1384
Translation	MFPRPLTPLAAPNGAEPLGRALRRAPLGRARAGLGGPPLLLPSMLMFAVIVASSGLLLMIERGILAEMKPLPLHPPGREGTAWRGKAPKPGGLSLRAGDADLQVRQDVRNRTLRAVCGQPGMPRDPWDLPVGQRRTLLRHILVSDRYRFLYCYVPKVACSNWKRVMKVLAGVLDSVDVRLKMDHRSDLVFLADLRPEEIRYRLQHYFKFLFVREPLERLLSAYRNKFGEIREYQQRYGAEIVRRYRAGAGPSPAGDDVTFPEFLRYLVDEDPERMNEHWMPVYHLCQPCAVHYDFVGSYERLEADANQVLEWVRAPPHVRFPARQAWYRPASPESLHYHLCSAPRALLQDVLPKYILDFSLFAYPLPNVTKEACQQ

Target ORF information:

RefSeq Version	NM_130468.3
Organism	Homo sapiens(human)
Definition	Homo sapiens carbohydrate sulfotransferase 14 (CHST14), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_130468.3

ORF Insert Sequence:

ATGTTCCCCC GCCCGCTGAC CCCGCTGGCG GCCCCAAATG GCGCCGAGCC CCTGGGCCGG 
GCGCTGAGGC GGGCCCCTCT GGGCAGGGCC CGGGCGGGGC TGGGTGGGCC GCCCCTGCTG 
CTGCCGTCCA TGCTGATGTT TGCGGTGATC GTGGCCTCCA GCGGGCTGCT GCTCATGATC 
GAGCGGGGCA TCCTGGCCGA GATGAAGCCC CTGCCCCTGC ACCCGCCCGG CCGCGAGGGC 
ACAGCCTGGC GCGGGAAAGC CCCCAAGCCT GGGGGCCTGT CCCTCAGGGC TGGGGACGCG 
GACTTGCAAG TGCGGCAGGA CGTCCGGAAC AGGACCCTGC GGGCGGTGTG CGGACAGCCA 
GGCATGCCCC GGGACCCCTG GGACTTGCCG GTGGGGCAGC GGCGCACCCT GCTGCGCCAC 
ATCCTCGTAA GTGACCGTTA CCGCTTCCTC TACTGCTACG TCCCCAAGGT GGCCTGCTCT 
AACTGGAAGC GGGTGATGAA GGTGCTGGCA GGCGTCCTGG ACAGCGTGGA CGTCCGCCTC 
AAGATGGACC ACCGCAGTGA CCTGGTGTTC CTGGCCGACC TGCGGCCTGA GGAGATTCGC 
TACCGCCTGC AGCACTACTT TAAGTTCCTG TTTGTGCGGG AGCCCTTGGA ACGCCTCCTC 
TCTGCCTACC GCAACAAGTT TGGCGAGATC CGAGAGTACC AGCAACGCTA TGGGGCTGAG 
ATAGTGAGGC GGTACAGGGC TGGAGCGGGG CCCAGCCCTG CAGGCGACGA TGTCACATTC 
CCCGAGTTCC TGAGATACCT GGTGGATGAG GACCCTGAGC GCATGAATGA GCATTGGATG 
CCCGTGTACC ACCTGTGCCA GCCTTGTGCC GTGCACTATG ACTTTGTGGG CTCCTATGAG 
AGGCTGGAGG CTGATGCAAA TCAGGTGCTG GAGTGGGTAC GGGCACCACC TCACGTCCGA 
TTTCCAGCTC GCCAGGCCTG GTACCGGCCA GCCAGCCCCG AAAGCCTGCA TTACCACTTG 
TGCAGTGCCC CCCGGGCCCT GCTGCAGGAT GTGCTGCCTA AGTATATCCT GGACTTCTCC 
CTCTTTGCCT ACCCACTGCC TAATGTCACC AAGGAGGCGT GTCAGCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Defect in dermatan sulfate in urine of patients with Ehlers-Danlos syndrome caused by a CHST14/D4ST1 deficiency.
Clinical biochemistry50(12)670-677(2017 Aug)
Mizumoto S,Kosho T,Hatamochi A,Honda T,Yamaguchi T,Okamoto N,Miyake N,Yamada S,Sugahara K

CHST14/D4ST1 deficiency: New form of Ehlers-Danlos syndrome.
Pediatrics international : official journal of the Japan Pediatric Society58(2)88-99(2016 Feb)
Kosho T

The phenotype of the musculocontractural type of Ehlers-Danlos syndrome due to CHST14 mutations.
American journal of medical genetics. Part A170A(1)103-15(2016 Jan)
Janecke AR,Li B,Boehm M,Krabichler B,Rohrbach M,M?ller T,Fuchs I,Golas G,Katagiri Y,Ziegler SG,Gahl WA,Wilnai Y,Zoppi N,Geller HM,Giunta C,Slavotinek A,Steinmann B

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CHST14 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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