PNKP cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PNKP
Entrez Gene ID	11284
Full Name	polynucleotide kinase 3'-phosphatase
Synonyms	AOA4,EIEE10,MCSZ,PNK
General protein information	Preferred Names polynucleotide kinase 3'-phosphatase Names bifunctional polynucleotide phosphatase/kinase DNA 5'-kinase/3'-phosphatase Homo sapiens polynucleotide kinase 3'-phosphatase (PNKP)
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 19 Map Location: 19q13.33
Summary	This locus represents a gene involved in DNA repair. In response to ionizing radiation or oxidative damage, the protein encoded by this locus catalyzes 5' phosphorylation and 3' dephosphorylation of nucleic acids. Mutations at this locus have been associated with microcephaly, seizures, and developmental delay.[provided by RefSeq, Sep 2010].
Disorder MIM:	605610
Disorder Html:	Microcephaly, seizures, and developmental delay, 613402 (3); Ataxia-oculomotor apraxia 4, 616267 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_007254.4	NP_009185.2	bifunctional polynucleotide phosphatase/kinase
NM_007254.3	NP_009185.2	bifunctional polynucleotide phosphatase/kinase

Pathways from BioSystems

Homologies

	PNKP	XP_001115581.1
Bos taurus (cattle)	PNKP	NP_001076991.1
Caenorhabditis elegans (roundworm)	F21D5.5	NP_501503.1
Schizosaccharomyces pombe (fission yeast)	pnk1	NP_593635.2
Neurospora crassa	NCU08151	XP_959562.2
Mus musculus (house mouse)	Pnkp	NP_067524.2
Magnaporthe oryzae (rice blast fungus)	MGG_09734	XP_003717618.1
Pan troglodytes (chimpanzee)	PNKP	XP_003316598.1
Canis lupus familiaris (dog)	PNKP	XP_541488.2
Rattus norvegicus (Norway rat)	Pnkp	NP_001004259.1
Xenopus tropicalis (tropical clawed frog)	pnkp	NP_001120633.1
Homo sapiens (human)	PNKP	NP_009185.2
Danio rerio (zebrafish)	pnkp	NP_001071046.1
Drosophila melanogaster (fruit fly)	CG9601	NP_649792.3

Gene Ontology
Bibliography

Related articles in PubMed

The Rev1 interacting region (RIR) motif in the scaffold protein XRCC1 mediates a low-affinity interaction with polynucleotide kinase/phosphatase (PNKP) during DNA single-strand break repair.
Breslin C, Mani RS, Fanta M, Hoch N, Weinfeld M, Caldecott KW
The Journal of biological chemistry292(39)16024-16031(2017 09)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

XRCC1 and PNKP interact via a high-affinity phosphorylation-dependent interaction site in XRCC1 and a forkhead-associated domain in PNKP. Data suggest a second PNKP interaction site in XRCC1 that binds PNKP with lower affinity and independently of XRCC1 phosphorylation. (XRCC1 = X-ray repair cross complementing protein 1; PNKP = polynucleotide kinase 3'-phosphatase)
Title: The Rev1 interacting region (RIR) motif in the scaffold protein XRCC1 mediates a low-affinity interaction with polynucleotide kinase/phosphatase (PNKP) during DNA single-strand break repair.

In a recombinant PNKP-XRCC4-LigIV complex, stable binding of PNKP requires XRCC4 phosphorylation. Only one PNKP protomer binds per XRCC4 dimer. Both the PNKP FHA and catalytic domains contact the XRCC4 coiled-coil and LigIV BRCT repeats. A surface on the PNKP phosphatase domain may contact XRCC4-LigIV. A mutation on this surface (E326K) impairs PNKP recruitment to damaged DNA and causes microcephaly with seizures.
Title: Structural and functional characterization of the PNKP-XRCC4-LigIV DNA repair complex.

the role for PNKP in maintaining brain function and how perturbation in its activity can account for the varied pathology of neurodegeneration or microcephaly present in microcephaly with seizures and ataxia with oculomotor apraxia 4 respectively.
Title: Polynucleotide kinase-phosphatase (PNKP) mutations and neurologic disease.

Mutations in TDP1 and APTX have been linked to Spinocerebellar ataxia with axonal neuropathy (SCAN1) and Ataxia-ocular motor apraxia 1 (AOA1), respectively, while mutations in PNKP are considered to be responsible for Microcephaly with seizures (MCSZ) and Ataxia-ocular motor apraxia 4 (AOA4).
Title: Neurological disorders associated with DNA strand-break processing enzymes.

we have identified a mutation in PNKP, leading to a phenotype of microcephaly with primordial dwarfism.
Title: Microcephalic primordial dwarfism in an Emirati patient with PNKP mutation.

The following PNKP gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PNKP cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu26989	NM_007254.4 Latest version!	Homo sapiens polynucleotide kinase 3'-phosphatase (PNKP), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
View Old Accession Versions >>
OHu26989	NM_007254.3	Homo sapiens polynucleotide kinase 3'-phosphatase (PNKP), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PNKP ( NM_007254.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_009185.2 Homo sapiens polynucleotide kinase 3'-phosphatase (PNKP), mRNA.

Price & Availability↑

CloneID	OHu26989
Clone ID Related Accession (Same CDS sequence)	NM_007254.4 , NM_007254.3
Accession Version	NM_007254.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1566bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	bifunctional polynucleotide phosphatase/kinase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC118341.2, BU541073.1 and BC033822.1. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_007254.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.200165.1, SRR3476690.320790.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000322344.8/ ENSP00000323511.2 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGGCGAGG TGGAGGCCCC GGGCCGCTTG TGGCTCGAGA GCCCCCCTGG GGGAGCGCCC 
CCCATCTTCC TGCCCTCGGA CGGGCAAGCC CTGGTCCTGG GCAGGGGACC CCTGACCCAG 
GTTACGGACC GGAAGTGCTC CAGAACTCAA GTGGAGCTGG TCGCAGATCC TGAGACCCGG 
ACAGTGGCAG TGAAACAGCT GGGAGTTAAC CCCTCAACTA CCGGGACCCA GGAGTTGAAG 
CCGGGGTTGG AGGGCTCTCT GGGGGTGGGG GACACACTGT ATTTGGTCAA TGGCCTCCAC 
CCACTGACCC TGCGCTGGGA AGAGACCCGC ACACCAGAAT CCCAGCCAGA TACTCCGCCT 
GGCACCCCTC TGGTGTCCCA AGATGAGAAG AGAGATGCTG AGCTGCCGAA GAAGCGTATG 
CGGAAGTCAA ACCCCGGCTG GGAGAACTTG GAGAAGTTGC TAGTGTTCAC CGCAGCTGGG 
GTGAAACCCC AGGGCAAGGT GGCTGGCTTT GATCTGGACG GGACGCTCAT CACCACACGC 
TCTGGGAAGG TCTTTCCCAC TGGCCCCAGT GACTGGAGGA TCTTGTACCC AGAGATTCCC 
CGTAAGCTCC GAGAGCTGGA AGCCGAGGGC TACAAGCTGG TGATCTTCAC CAACCAGATG 
AGCATCGGGC GCGGGAAGCT GCCAGCCGAG GAGTTCAAGG CCAAGGTGGA GGCTGTGGTG 
GAGAAGCTGG GGGTCCCCTT CCAGGTGCTG GTGGCCACGC ACGCAGGCTT GTACCGGAAG 
CCGGTGACGG GCATGTGGGA CCATCTGCAG GAGCAGGCCA ACGACGGCAC GCCCATATCC 
ATCGGGGACA GCATCTTTGT GGGAGACGCA GCCGGACGCC CGGCCAACTG GGCCCCGGGG 
CGGAAGAAGA AAGACTTCTC CTGCGCCGAT CGCCTGTTTG CCCTCAACCT TGGCCTGCCC 
TTCGCCACGC CTGAGGAGTT CTTTCTCAAG TGGCCAGCAG CCGGCTTCGA GCTCCCAGCC 
TTTGATCCGA GGACTGTCTC CCGCTCAGGG CCTCTCTGCC TCCCCGAGTC CAGGGCCCTC 
CTGAGCGCCA GCCCGGAGGT GGTTGTCGCA GTGGGATTCC CTGGGGCCGG GAAGTCCACC 
TTTCTCAAGA AGCACCTCGT GTCGGCCGGA TATGTCCACG TGAACAGGGA CACGCTAGGC 
TCCTGGCAGC GCTGTGTGAC CACGTGTGAG ACAGCCCTGA AGCAAGGGAA ACGGGTCGCC 
ATCGACAACA CAAACCCAGA CGCCGCGAGC CGCGCCAGGT ACGTCCAGTG TGCCCGAGCC 
GCGGGCGTCC CCTGCCGCTG CTTCCTCTTC ACCGCCACTC TGGAGCAGGC GCGCCACAAC 
AACCGGTTTC GAGAGATGAC GGACTCCTCT CATATCCCCG TGTCAGACAT GGTCATGTAT 
GGCTACAGGA AGCAGTTCGA GGCCCCAACG CTGGCTGAAG GCTTCTCTGC CATCCTGGAG 
ATCCCGTTCC GGCTATGGGT GGAGCCGAGG CTGGGGCGGC TGTACTGCCA GTTCTCCGAG 
GGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_009185.2
CDS	122..1687
Translation	MGEVEAPGRLWLESPPGGAPPIFLPSDGQALVLGRGPLTQVTDRKCSRTQVELVADPETRTVAVKQLGVNPSTTGTQELKPGLEGSLGVGDTLYLVNGLHPLTLRWEETRTPESQPDTPPGTPLVSQDEKRDAELPKKRMRKSNPGWENLEKLLVFTAAGVKPQGKVAGFDLDGTLITTRSGKVFPTGPSDWRILYPEIPRKLRELEAEGYKLVIFTNQMSIGRGKLPAEEFKAKVEAVVEKLGVPFQVLVATHAGLYRKPVTGMWDHLQEQANDGTPISIGDSIFVGDAAGRPANWAPGRKKKDFSCADRLFALNLGLPFATPEEFFLKWPAAGFELPAFDPRTVSRSGPLCLPESRALLSASPEVVVAVGFPGAGKSTFLKKHLVSAGYVHVNRDTLGSWQRCVTTCETALKQGKRVAIDNTNPDAASRARYVQCARAAGVPCRCFLFTATLEQARHNNRFREMTDSSHIPVSDMVMYGYRKQFEAPTLAEGFSAILEIPFRLWVEPRLGRLYCQFSEG

Target ORF information:

RefSeq Version	NM_007254.4
Organism	Homo sapiens(human)
Definition	Homo sapiens polynucleotide kinase 3'-phosphatase (PNKP), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007254.4

ORF Insert Sequence:

ATGGGCGAGG TGGAGGCCCC GGGCCGCTTG TGGCTCGAGA GCCCCCCTGG GGGAGCGCCC 
CCCATCTTCC TGCCCTCGGA CGGGCAAGCC CTGGTCCTGG GCAGGGGACC CCTGACCCAG 
GTTACGGACC GGAAGTGCTC CAGAACTCAA GTGGAGCTGG TCGCAGATCC TGAGACCCGG 
ACAGTGGCAG TGAAACAGCT GGGAGTTAAC CCCTCAACTA CCGGGACCCA GGAGTTGAAG 
CCGGGGTTGG AGGGCTCTCT GGGGGTGGGG GACACACTGT ATTTGGTCAA TGGCCTCCAC 
CCACTGACCC TGCGCTGGGA AGAGACCCGC ACACCAGAAT CCCAGCCAGA TACTCCGCCT 
GGCACCCCTC TGGTGTCCCA AGATGAGAAG AGAGATGCTG AGCTGCCGAA GAAGCGTATG 
CGGAAGTCAA ACCCCGGCTG GGAGAACTTG GAGAAGTTGC TAGTGTTCAC CGCAGCTGGG 
GTGAAACCCC AGGGCAAGGT GGCTGGCTTT GATCTGGACG GGACGCTCAT CACCACACGC 
TCTGGGAAGG TCTTTCCCAC TGGCCCCAGT GACTGGAGGA TCTTGTACCC AGAGATTCCC 
CGTAAGCTCC GAGAGCTGGA AGCCGAGGGC TACAAGCTGG TGATCTTCAC CAACCAGATG 
AGCATCGGGC GCGGGAAGCT GCCAGCCGAG GAGTTCAAGG CCAAGGTGGA GGCTGTGGTG 
GAGAAGCTGG GGGTCCCCTT CCAGGTGCTG GTGGCCACGC ACGCAGGCTT GTACCGGAAG 
CCGGTGACGG GCATGTGGGA CCATCTGCAG GAGCAGGCCA ACGACGGCAC GCCCATATCC 
ATCGGGGACA GCATCTTTGT GGGAGACGCA GCCGGACGCC CGGCCAACTG GGCCCCGGGG 
CGGAAGAAGA AAGACTTCTC CTGCGCCGAT CGCCTGTTTG CCCTCAACCT TGGCCTGCCC 
TTCGCCACGC CTGAGGAGTT CTTTCTCAAG TGGCCAGCAG CCGGCTTCGA GCTCCCAGCC 
TTTGATCCGA GGACTGTCTC CCGCTCAGGG CCTCTCTGCC TCCCCGAGTC CAGGGCCCTC 
CTGAGCGCCA GCCCGGAGGT GGTTGTCGCA GTGGGATTCC CTGGGGCCGG GAAGTCCACC 
TTTCTCAAGA AGCACCTCGT GTCGGCCGGA TATGTCCACG TGAACAGGGA CACGCTAGGC 
TCCTGGCAGC GCTGTGTGAC CACGTGTGAG ACAGCCCTGA AGCAAGGGAA ACGGGTCGCC 
ATCGACAACA CAAACCCAGA CGCCGCGAGC CGCGCCAGGT ACGTCCAGTG TGCCCGAGCC 
GCGGGCGTCC CCTGCCGCTG CTTCCTCTTC ACCGCCACTC TGGAGCAGGC GCGCCACAAC 
AACCGGTTTC GAGAGATGAC GGACTCCTCT CATATCCCCG TGTCAGACAT GGTCATGTAT 
GGCTACAGGA AGCAGTTCGA GGCCCCAACG CTGGCTGAAG GCTTCTCTGC CATCCTGGAG 
ATCCCGTTCC GGCTATGGGT GGAGCCGAGG CTGGGGCGGC TGTACTGCCA GTTCTCCGAG 
GGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PNKP ( NM_007254.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_009185.2 Homo sapiens polynucleotide kinase 3'-phosphatase (PNKP), mRNA.

Price & Availability↑

CloneID	OHu26989
Clone ID Related Accession (Same CDS sequence)	NM_007254.4 , NM_007254.3
Accession Version	NM_007254.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1566bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-23
Organism	Homo sapiens(human)
Product	bifunctional polynucleotide phosphatase/kinase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BU541073.1, BC033822.1 and AA640353.1. This sequence is a reference standard in the RefSeqGene project. On Sep 9, 2010 this sequence version replaced NM_007254.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC033822.1, ERR279859.1247.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGCGAGG TGGAGGCCCC GGGCCGCTTG TGGCTCGAGA GCCCCCCTGG GGGAGCGCCC 
CCCATCTTCC TGCCCTCGGA CGGGCAAGCC CTGGTCCTGG GCAGGGGACC CCTGACCCAG 
GTTACGGACC GGAAGTGCTC CAGAACTCAA GTGGAGCTGG TCGCAGATCC TGAGACCCGG 
ACAGTGGCAG TGAAACAGCT GGGAGTTAAC CCCTCAACTA CCGGGACCCA GGAGTTGAAG 
CCGGGGTTGG AGGGCTCTCT GGGGGTGGGG GACACACTGT ATTTGGTCAA TGGCCTCCAC 
CCACTGACCC TGCGCTGGGA AGAGACCCGC ACACCAGAAT CCCAGCCAGA TACTCCGCCT 
GGCACCCCTC TGGTGTCCCA AGATGAGAAG AGAGATGCTG AGCTGCCGAA GAAGCGTATG 
CGGAAGTCAA ACCCCGGCTG GGAGAACTTG GAGAAGTTGC TAGTGTTCAC CGCAGCTGGG 
GTGAAACCCC AGGGCAAGGT GGCTGGCTTT GATCTGGACG GGACGCTCAT CACCACACGC 
TCTGGGAAGG TCTTTCCCAC TGGCCCCAGT GACTGGAGGA TCTTGTACCC AGAGATTCCC 
CGTAAGCTCC GAGAGCTGGA AGCCGAGGGC TACAAGCTGG TGATCTTCAC CAACCAGATG 
AGCATCGGGC GCGGGAAGCT GCCAGCCGAG GAGTTCAAGG CCAAGGTGGA GGCTGTGGTG 
GAGAAGCTGG GGGTCCCCTT CCAGGTGCTG GTGGCCACGC ACGCAGGCTT GTACCGGAAG 
CCGGTGACGG GCATGTGGGA CCATCTGCAG GAGCAGGCCA ACGACGGCAC GCCCATATCC 
ATCGGGGACA GCATCTTTGT GGGAGACGCA GCCGGACGCC CGGCCAACTG GGCCCCGGGG 
CGGAAGAAGA AAGACTTCTC CTGCGCCGAT CGCCTGTTTG CCCTCAACCT TGGCCTGCCC 
TTCGCCACGC CTGAGGAGTT CTTTCTCAAG TGGCCAGCAG CCGGCTTCGA GCTCCCAGCC 
TTTGATCCGA GGACTGTCTC CCGCTCAGGG CCTCTCTGCC TCCCCGAGTC CAGGGCCCTC 
CTGAGCGCCA GCCCGGAGGT GGTTGTCGCA GTGGGATTCC CTGGGGCCGG GAAGTCCACC 
TTTCTCAAGA AGCACCTCGT GTCGGCCGGA TATGTCCACG TGAACAGGGA CACGCTAGGC 
TCCTGGCAGC GCTGTGTGAC CACGTGTGAG ACAGCCCTGA AGCAAGGGAA ACGGGTCGCC 
ATCGACAACA CAAACCCAGA CGCCGCGAGC CGCGCCAGGT ACGTCCAGTG TGCCCGAGCC 
GCGGGCGTCC CCTGCCGCTG CTTCCTCTTC ACCGCCACTC TGGAGCAGGC GCGCCACAAC 
AACCGGTTTC GAGAGATGAC GGACTCCTCT CATATCCCCG TGTCAGACAT GGTCATGTAT 
GGCTACAGGA AGCAGTTCGA GGCCCCAACG CTGGCTGAAG GCTTCTCTGC CATCCTGGAG 
ATCCCGTTCC GGCTATGGGT GGAGCCGAGG CTGGGGCGGC TGTACTGCCA GTTCTCCGAG 
GGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_009185.2
CDS	111..1676
Translation	MGEVEAPGRLWLESPPGGAPPIFLPSDGQALVLGRGPLTQVTDRKCSRTQVELVADPETRTVAVKQLGVNPSTTGTQELKPGLEGSLGVGDTLYLVNGLHPLTLRWEETRTPESQPDTPPGTPLVSQDEKRDAELPKKRMRKSNPGWENLEKLLVFTAAGVKPQGKVAGFDLDGTLITTRSGKVFPTGPSDWRILYPEIPRKLRELEAEGYKLVIFTNQMSIGRGKLPAEEFKAKVEAVVEKLGVPFQVLVATHAGLYRKPVTGMWDHLQEQANDGTPISIGDSIFVGDAAGRPANWAPGRKKKDFSCADRLFALNLGLPFATPEEFFLKWPAAGFELPAFDPRTVSRSGPLCLPESRALLSASPEVVVAVGFPGAGKSTFLKKHLVSAGYVHVNRDTLGSWQRCVTTCETALKQGKRVAIDNTNPDAASRARYVQCARAAGVPCRCFLFTATLEQARHNNRFREMTDSSHIPVSDMVMYGYRKQFEAPTLAEGFSAILEIPFRLWVEPRLGRLYCQFSEG

Target ORF information:

RefSeq Version	NM_007254.3
Organism	Homo sapiens(human)
Definition	Homo sapiens polynucleotide kinase 3'-phosphatase (PNKP), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007254.3

ORF Insert Sequence:

ATGGGCGAGG TGGAGGCCCC GGGCCGCTTG TGGCTCGAGA GCCCCCCTGG GGGAGCGCCC 
CCCATCTTCC TGCCCTCGGA CGGGCAAGCC CTGGTCCTGG GCAGGGGACC CCTGACCCAG 
GTTACGGACC GGAAGTGCTC CAGAACTCAA GTGGAGCTGG TCGCAGATCC TGAGACCCGG 
ACAGTGGCAG TGAAACAGCT GGGAGTTAAC CCCTCAACTA CCGGGACCCA GGAGTTGAAG 
CCGGGGTTGG AGGGCTCTCT GGGGGTGGGG GACACACTGT ATTTGGTCAA TGGCCTCCAC 
CCACTGACCC TGCGCTGGGA AGAGACCCGC ACACCAGAAT CCCAGCCAGA TACTCCGCCT 
GGCACCCCTC TGGTGTCCCA AGATGAGAAG AGAGATGCTG AGCTGCCGAA GAAGCGTATG 
CGGAAGTCAA ACCCCGGCTG GGAGAACTTG GAGAAGTTGC TAGTGTTCAC CGCAGCTGGG 
GTGAAACCCC AGGGCAAGGT GGCTGGCTTT GATCTGGACG GGACGCTCAT CACCACACGC 
TCTGGGAAGG TCTTTCCCAC TGGCCCCAGT GACTGGAGGA TCTTGTACCC AGAGATTCCC 
CGTAAGCTCC GAGAGCTGGA AGCCGAGGGC TACAAGCTGG TGATCTTCAC CAACCAGATG 
AGCATCGGGC GCGGGAAGCT GCCAGCCGAG GAGTTCAAGG CCAAGGTGGA GGCTGTGGTG 
GAGAAGCTGG GGGTCCCCTT CCAGGTGCTG GTGGCCACGC ACGCAGGCTT GTACCGGAAG 
CCGGTGACGG GCATGTGGGA CCATCTGCAG GAGCAGGCCA ACGACGGCAC GCCCATATCC 
ATCGGGGACA GCATCTTTGT GGGAGACGCA GCCGGACGCC CGGCCAACTG GGCCCCGGGG 
CGGAAGAAGA AAGACTTCTC CTGCGCCGAT CGCCTGTTTG CCCTCAACCT TGGCCTGCCC 
TTCGCCACGC CTGAGGAGTT CTTTCTCAAG TGGCCAGCAG CCGGCTTCGA GCTCCCAGCC 
TTTGATCCGA GGACTGTCTC CCGCTCAGGG CCTCTCTGCC TCCCCGAGTC CAGGGCCCTC 
CTGAGCGCCA GCCCGGAGGT GGTTGTCGCA GTGGGATTCC CTGGGGCCGG GAAGTCCACC 
TTTCTCAAGA AGCACCTCGT GTCGGCCGGA TATGTCCACG TGAACAGGGA CACGCTAGGC 
TCCTGGCAGC GCTGTGTGAC CACGTGTGAG ACAGCCCTGA AGCAAGGGAA ACGGGTCGCC 
ATCGACAACA CAAACCCAGA CGCCGCGAGC CGCGCCAGGT ACGTCCAGTG TGCCCGAGCC 
GCGGGCGTCC CCTGCCGCTG CTTCCTCTTC ACCGCCACTC TGGAGCAGGC GCGCCACAAC 
AACCGGTTTC GAGAGATGAC GGACTCCTCT CATATCCCCG TGTCAGACAT GGTCATGTAT 
GGCTACAGGA AGCAGTTCGA GGCCCCAACG CTGGCTGAAG GCTTCTCTGC CATCCTGGAG 
ATCCCGTTCC GGCTATGGGT GGAGCCGAGG CTGGGGCGGC TGTACTGCCA GTTCTCCGAG 
GGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The Rev1 interacting region (RIR) motif in the scaffold protein XRCC1 mediates a low-affinity interaction with polynucleotide kinase/phosphatase (PNKP) during DNA single-strand break repair.
The Journal of biological chemistry292(39)16024-16031(2017 09)
Breslin C,Mani RS,Fanta M,Hoch N,Weinfeld M,Caldecott KW

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PNKP cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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