CHRM1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	CHRM1
Entrez Gene ID	1128
Full Name	cholinergic receptor muscarinic 1
Synonyms	HM1,M1,M1R
General protein information	Preferred Names cholinergic receptor muscarinic 1 Names muscarinic acetylcholine receptor M1 acetylcholine receptor, muscarinic 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 11 Map Location: 11q12.3
Summary	The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The muscarinic cholinergic receptor 1 is involved in mediation of vagally-induced bronchoconstriction and in the acid secretion of the gastrointestinal tract. The gene encoding this receptor is localized to 11q13. [provided by RefSeq, Jul 2008].
Disorder MIM:	118510

mRNA and Protein(s)

mRNA	Protein	Name
NM_000738.2	NP_000729.2	muscarinic acetylcholine receptor M1
NM_000738.3	NP_000729.2	muscarinic acetylcholine receptor M1
XM_011544742.1	XP_011543044.1	muscarinic acetylcholine receptor M1 isoform X1
XM_011544742.2	XP_011543044.1	muscarinic acetylcholine receptor M1 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	CHRM1	NP_000729.2
Pan troglodytes (chimpanzee)	CHRM1	XP_508508.2
Mus musculus (house mouse)	Chrm1	NP_001106167.1
Rattus norvegicus (Norway rat)	Chrm1	NP_542951.1
Canis lupus familiaris (dog)	CHRM1	XP_005631654.1
Caenorhabditis elegans (roundworm)	gar-3	NP_001024236.1
Xenopus tropicalis (tropical clawed frog)	LOC100490434	XP_004913718.1
	CHRM1	NP_001028117.1
Bos taurus (cattle)	CHRM1	NP_001231538.1
Drosophila melanogaster (fruit fly)	mAcR-60C	NP_726440.1
Anopheles gambiae (African malaria mosquito)	GPRMAC1	XP_314486.1

Gene Ontology
Bibliography

Related articles in PubMed

Evidence of a M
Baig AM, Ahmad HR
Journal of receptor and signal transduction research37(3)267-275(2017 Jun)

Receptor Species-dependent Desensitization Controls KCNQ1/KCNE1 K+ Channels as Downstream Effectors of Gq Protein-coupled Receptors.
Kienitz MC, Vladimirova D, M?ller C, Pott L, Rinne A
The Journal of biological chemistry291(51)26410-26426(2016 Dec)

Association of the Cholinergic Muscarinic M2 Receptor with Autonomic Nervous System Activity in Patients with Schizophrenia on High-Dose Antipsychotics.
Miyauchi M, Kishida I, Suda A, Shiraishi Y, Hattori S, Fujibayashi M, Taguri M, Ishii C, Ishii N, Moritani T, Hirayasu Y
Neuropsychobiology74(1)60-67(2016)

Enhancing the Reliability of GPCR Models by Accounting for Flexibility of Their Pro-Containing Helices: the Case of the Human mAChR1 Receptor.
Pedretti A, Mazzolari A, Ricci C, Vistoli G
Molecular informatics34(4)216-27(2015 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

he discovery of a receptor homolog of human mAChR1 on Acanthamoeba with future studies planned to show its expression and binding to cholinergic agonist and antagonist would help clarify its role in the biology of this protist pathogen.
Title: Evidence of a M

Receptor Species-dependent Desensitization Controls KCNQ1/KCNE1 K+ Channels as Downstream Effectors of Gq Protein-coupled Receptors.(
Title: Receptor Species-dependent Desensitization Controls KCNQ1/KCNE1 K+ Channels as Downstream Effectors of Gq Protein-coupled Receptors.

crystal structures of the M1 and M4 muscarinic receptors bound to the inverse agonist, tiotropium
Title: Crystal structures of the M1 and M4 muscarinic acetylcholine receptors.

There were no significant associations between CHRM1 SNPs and autonomic nervous system activity in patients with schizophrenia on high-dose antipsychotics.
Title: Association of the Cholinergic Muscarinic M2 Receptor with Autonomic Nervous System Activity in Patients with Schizophrenia on High-Dose Antipsychotics.

The study was focused on the human mAChR1 receptor for which a presumably reliable model was generated using the congener mAChR3 as the template along with a second less reliable model based on the distant beta2-AR template. The second model was then utilized to produce the chimeras by combining the conformations of its Pro-containing helices (i.e., TM4, TM5, TM6 and TM7 with 16 modeled chimeras).
Title: Enhancing the Reliability of GPCR Models by Accounting for Flexibility of Their Pro-Containing Helices: the Case of the Human mAChR1 Receptor.

The following CHRM1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CHRM1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu25587	NM_000738.3 Latest version!	Homo sapiens cholinergic receptor muscarinic 1 (CHRM1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu25587	XM_011544742.2 Latest version!	Homo sapiens cholinergic receptor muscarinic 1 (CHRM1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
View Old Accession Versions >>
OHu25587	NM_000738.2	Homo sapiens cholinergic receptor muscarinic 1 (CHRM1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu25587	XM_011544742.1	Homo sapiens cholinergic receptor, muscarinic 1 (CHRM1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CHRM1 ( NM_000738.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_000729.2 Homo sapiens cholinergic receptor muscarinic 1 (CHRM1), mRNA.

Price & Availability↑

CloneID	OHu25587
Clone ID Related Accession (Same CDS sequence)	NM_000738.2 , NM_000738.3 , XM_011544742.1 , XM_011544742.2
Accession Version	NM_000738.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1383bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-26
Organism	Homo sapiens(human)
Product	muscarinic acetylcholine receptor M1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC022984.1. On Oct 10, 2003 this sequence version replaced NM_000738.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC022984.1, SRR1803616.202758.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAACACTT CAGCCCCACC TGCTGTCAGC CCCAACATCA CCGTCCTGGC ACCAGGAAAG 
GGTCCCTGGC AAGTGGCCTT CATTGGGATC ACCACGGGCC TCCTGTCGCT AGCCACAGTG 
ACAGGCAACC TGCTGGTACT CATCTCTTTC AAGGTCAACA CGGAGCTCAA GACAGTCAAT 
AACTACTTCC TGCTGAGCCT GGCCTGTGCT GACCTCATCA TCGGTACCTT CTCCATGAAC 
CTCTATACCA CGTACCTGCT CATGGGCCAC TGGGCTCTGG GCACGCTGGC TTGTGACCTC 
TGGCTGGCCC TGGACTATGT GGCCAGCAAT GCCTCCGTCA TGAATCTGCT GCTCATCAGC 
TTTGACCGCT ACTTCTCCGT GACTCGGCCC CTGAGCTACC GTGCCAAGCG CACACCCCGC 
CGGGCAGCTC TGATGATCGG CCTGGCCTGG CTGGTTTCCT TTGTGCTCTG GGCCCCAGCC 
ATCCTCTTCT GGCAGTACCT GGTAGGGGAG CGGACAGTGC TAGCTGGGCA GTGCTACATC 
CAGTTCCTCT CCCAGCCCAT CATCACCTTT GGCACAGCCA TGGCTGCCTT CTACCTCCCT 
GTCACAGTCA TGTGCACGCT CTACTGGCGC ATCTACCGGG AGACAGAGAA CCGAGCACGG 
GAGCTGGCAG CCCTTCAGGG CTCCGAGACG CCAGGCAAAG GGGGTGGCAG CAGCAGCAGC 
TCAGAGAGGT CTCAGCCAGG GGCTGAGGGC TCACCAGAGA CTCCTCCAGG CCGCTGCTGT 
CGCTGCTGCC GGGCCCCCAG GCTGCTGCAG GCCTACAGCT GGAAGGAAGA AGAGGAAGAG 
GACGAAGGCT CCATGGAGTC CCTCACATCC TCAGAGGGAG AGGAGCCTGG CTCCGAAGTG 
GTGATCAAGA TGCCAATGGT GGACCCCGAG GCACAGGCCC CCACCAAGCA GCCCCCACGG 
AGCTCCCCAA ATACAGTCAA GAGGCCGACT AAGAAAGGGC GTGATCGAGC TGGCAAGGGC 
CAGAAGCCCC GTGGAAAGGA GCAGCTGGCC AAGCGGAAGA CCTTCTCGCT GGTCAAGGAG 
AAGAAGGCGG CTCGGACCCT GAGTGCCATC CTCCTGGCCT TCATCCTCAC CTGGACACCG 
TACAACATCA TGGTGCTGGT GTCCACCTTC TGCAAGGACT GTGTTCCCGA GACCCTGTGG 
GAGCTGGGCT ACTGGCTGTG CTACGTCAAC AGCACCATCA ACCCCATGTG CTACGCACTC 
TGCAACAAAG CCTTCCGGGA CACCTTTCGC CTGCTGCTGC TTTGCCGCTG GGACAAGAGA 
CGCTGGCGCA AGATCCCCAA GCGCCCTGGC TCCGTGCACC GCACTCCCTC CCGCCAATGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000729.2
CDS	402..1784
Translation	MNTSAPPAVSPNITVLAPGKGPWQVAFIGITTGLLSLATVTGNLLVLISFKVNTELKTVNNYFLLSLACADLIIGTFSMNLYTTYLLMGHWALGTLACDLWLALDYVASNASVMNLLLISFDRYFSVTRPLSYRAKRTPRRAALMIGLAWLVSFVLWAPAILFWQYLVGERTVLAGQCYIQFLSQPIITFGTAMAAFYLPVTVMCTLYWRIYRETENRARELAALQGSETPGKGGGSSSSSERSQPGAEGSPETPPGRCCRCCRAPRLLQAYSWKEEEEEDEGSMESLTSSEGEEPGSEVVIKMPMVDPEAQAPTKQPPRSSPNTVKRPTKKGRDRAGKGQKPRGKEQLAKRKTFSLVKEKKAARTLSAILLAFILTWTPYNIMVLVSTFCKDCVPETLWELGYWLCYVNSTINPMCYALCNKAFRDTFRLLLLCRWDKRRWRKIPKRPGSVHRTPSRQC

Target ORF information:

RefSeq Version	NM_000738.2
Organism	Homo sapiens(human)
Definition	Homo sapiens cholinergic receptor muscarinic 1 (CHRM1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000738.2

ORF Insert Sequence:

ATGAACACTT CAGCCCCACC TGCTGTCAGC CCCAACATCA CCGTCCTGGC ACCAGGAAAG 
GGTCCCTGGC AAGTGGCCTT CATTGGGATC ACCACGGGCC TCCTGTCGCT AGCCACAGTG 
ACAGGCAACC TGCTGGTACT CATCTCTTTC AAGGTCAACA CGGAGCTCAA GACAGTCAAT 
AACTACTTCC TGCTGAGCCT GGCCTGTGCT GACCTCATCA TCGGTACCTT CTCCATGAAC 
CTCTATACCA CGTACCTGCT CATGGGCCAC TGGGCTCTGG GCACGCTGGC TTGTGACCTC 
TGGCTGGCCC TGGACTATGT GGCCAGCAAT GCCTCCGTCA TGAATCTGCT GCTCATCAGC 
TTTGACCGCT ACTTCTCCGT GACTCGGCCC CTGAGCTACC GTGCCAAGCG CACACCCCGC 
CGGGCAGCTC TGATGATCGG CCTGGCCTGG CTGGTTTCCT TTGTGCTCTG GGCCCCAGCC 
ATCCTCTTCT GGCAGTACCT GGTAGGGGAG CGGACAGTGC TAGCTGGGCA GTGCTACATC 
CAGTTCCTCT CCCAGCCCAT CATCACCTTT GGCACAGCCA TGGCTGCCTT CTACCTCCCT 
GTCACAGTCA TGTGCACGCT CTACTGGCGC ATCTACCGGG AGACAGAGAA CCGAGCACGG 
GAGCTGGCAG CCCTTCAGGG CTCCGAGACG CCAGGCAAAG GGGGTGGCAG CAGCAGCAGC 
TCAGAGAGGT CTCAGCCAGG GGCTGAGGGC TCACCAGAGA CTCCTCCAGG CCGCTGCTGT 
CGCTGCTGCC GGGCCCCCAG GCTGCTGCAG GCCTACAGCT GGAAGGAAGA AGAGGAAGAG 
GACGAAGGCT CCATGGAGTC CCTCACATCC TCAGAGGGAG AGGAGCCTGG CTCCGAAGTG 
GTGATCAAGA TGCCAATGGT GGACCCCGAG GCACAGGCCC CCACCAAGCA GCCCCCACGG 
AGCTCCCCAA ATACAGTCAA GAGGCCGACT AAGAAAGGGC GTGATCGAGC TGGCAAGGGC 
CAGAAGCCCC GTGGAAAGGA GCAGCTGGCC AAGCGGAAGA CCTTCTCGCT GGTCAAGGAG 
AAGAAGGCGG CTCGGACCCT GAGTGCCATC CTCCTGGCCT TCATCCTCAC CTGGACACCG 
TACAACATCA TGGTGCTGGT GTCCACCTTC TGCAAGGACT GTGTTCCCGA GACCCTGTGG 
GAGCTGGGCT ACTGGCTGTG CTACGTCAAC AGCACCATCA ACCCCATGTG CTACGCACTC 
TGCAACAAAG CCTTCCGGGA CACCTTTCGC CTGCTGCTGC TTTGCCGCTG GGACAAGAGA 
CGCTGGCGCA AGATCCCCAA GCGCCCTGGC TCCGTGCACC GCACTCCCTC CCGCCAATGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHRM1 ( NM_000738.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_000729.2 Homo sapiens cholinergic receptor muscarinic 1 (CHRM1), mRNA.

Price & Availability↑

CloneID	OHu25587
Clone ID Related Accession (Same CDS sequence)	NM_000738.2 , NM_000738.3 , XM_011544742.1 , XM_011544742.2
Accession Version	NM_000738.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1383bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-23
Organism	Homo sapiens(human)
Product	muscarinic acetylcholine receptor M1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC022984.1. On Nov 22, 2018 this sequence version replaced NM_000738.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC022984.1, SRR1803611.214381.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000306960.4/ ENSP00000306490.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGAACACTT CAGCCCCACC TGCTGTCAGC CCCAACATCA CCGTCCTGGC ACCAGGAAAG 
GGTCCCTGGC AAGTGGCCTT CATTGGGATC ACCACGGGCC TCCTGTCGCT AGCCACAGTG 
ACAGGCAACC TGCTGGTACT CATCTCTTTC AAGGTCAACA CGGAGCTCAA GACAGTCAAT 
AACTACTTCC TGCTGAGCCT GGCCTGTGCT GACCTCATCA TCGGTACCTT CTCCATGAAC 
CTCTATACCA CGTACCTGCT CATGGGCCAC TGGGCTCTGG GCACGCTGGC TTGTGACCTC 
TGGCTGGCCC TGGACTATGT GGCCAGCAAT GCCTCCGTCA TGAATCTGCT GCTCATCAGC 
TTTGACCGCT ACTTCTCCGT GACTCGGCCC CTGAGCTACC GTGCCAAGCG CACACCCCGC 
CGGGCAGCTC TGATGATCGG CCTGGCCTGG CTGGTTTCCT TTGTGCTCTG GGCCCCAGCC 
ATCCTCTTCT GGCAGTACCT GGTAGGGGAG CGGACAGTGC TAGCTGGGCA GTGCTACATC 
CAGTTCCTCT CCCAGCCCAT CATCACCTTT GGCACAGCCA TGGCTGCCTT CTACCTCCCT 
GTCACAGTCA TGTGCACGCT CTACTGGCGC ATCTACCGGG AGACAGAGAA CCGAGCACGG 
GAGCTGGCAG CCCTTCAGGG CTCCGAGACG CCAGGCAAAG GGGGTGGCAG CAGCAGCAGC 
TCAGAGAGGT CTCAGCCAGG GGCTGAGGGC TCACCAGAGA CTCCTCCAGG CCGCTGCTGT 
CGCTGCTGCC GGGCCCCCAG GCTGCTGCAG GCCTACAGCT GGAAGGAAGA AGAGGAAGAG 
GACGAAGGCT CCATGGAGTC CCTCACATCC TCAGAGGGAG AGGAGCCTGG CTCCGAAGTG 
GTGATCAAGA TGCCAATGGT GGACCCCGAG GCACAGGCCC CCACCAAGCA GCCCCCACGG 
AGCTCCCCAA ATACAGTCAA GAGGCCGACT AAGAAAGGGC GTGATCGAGC TGGCAAGGGC 
CAGAAGCCCC GTGGAAAGGA GCAGCTGGCC AAGCGGAAGA CCTTCTCGCT GGTCAAGGAG 
AAGAAGGCGG CTCGGACCCT GAGTGCCATC CTCCTGGCCT TCATCCTCAC CTGGACACCG 
TACAACATCA TGGTGCTGGT GTCCACCTTC TGCAAGGACT GTGTTCCCGA GACCCTGTGG 
GAGCTGGGCT ACTGGCTGTG CTACGTCAAC AGCACCATCA ACCCCATGTG CTACGCACTC 
TGCAACAAAG CCTTCCGGGA CACCTTTCGC CTGCTGCTGC TTTGCCGCTG GGACAAGAGA 
CGCTGGCGCA AGATCCCCAA GCGCCCTGGC TCCGTGCACC GCACTCCCTC CCGCCAATGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000729.2
CDS	225..1607
Translation	MNTSAPPAVSPNITVLAPGKGPWQVAFIGITTGLLSLATVTGNLLVLISFKVNTELKTVNNYFLLSLACADLIIGTFSMNLYTTYLLMGHWALGTLACDLWLALDYVASNASVMNLLLISFDRYFSVTRPLSYRAKRTPRRAALMIGLAWLVSFVLWAPAILFWQYLVGERTVLAGQCYIQFLSQPIITFGTAMAAFYLPVTVMCTLYWRIYRETENRARELAALQGSETPGKGGGSSSSSERSQPGAEGSPETPPGRCCRCCRAPRLLQAYSWKEEEEEDEGSMESLTSSEGEEPGSEVVIKMPMVDPEAQAPTKQPPRSSPNTVKRPTKKGRDRAGKGQKPRGKEQLAKRKTFSLVKEKKAARTLSAILLAFILTWTPYNIMVLVSTFCKDCVPETLWELGYWLCYVNSTINPMCYALCNKAFRDTFRLLLLCRWDKRRWRKIPKRPGSVHRTPSRQC

Target ORF information:

RefSeq Version	NM_000738.3
Organism	Homo sapiens(human)
Definition	Homo sapiens cholinergic receptor muscarinic 1 (CHRM1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000738.3

ORF Insert Sequence:

ATGAACACTT CAGCCCCACC TGCTGTCAGC CCCAACATCA CCGTCCTGGC ACCAGGAAAG 
GGTCCCTGGC AAGTGGCCTT CATTGGGATC ACCACGGGCC TCCTGTCGCT AGCCACAGTG 
ACAGGCAACC TGCTGGTACT CATCTCTTTC AAGGTCAACA CGGAGCTCAA GACAGTCAAT 
AACTACTTCC TGCTGAGCCT GGCCTGTGCT GACCTCATCA TCGGTACCTT CTCCATGAAC 
CTCTATACCA CGTACCTGCT CATGGGCCAC TGGGCTCTGG GCACGCTGGC TTGTGACCTC 
TGGCTGGCCC TGGACTATGT GGCCAGCAAT GCCTCCGTCA TGAATCTGCT GCTCATCAGC 
TTTGACCGCT ACTTCTCCGT GACTCGGCCC CTGAGCTACC GTGCCAAGCG CACACCCCGC 
CGGGCAGCTC TGATGATCGG CCTGGCCTGG CTGGTTTCCT TTGTGCTCTG GGCCCCAGCC 
ATCCTCTTCT GGCAGTACCT GGTAGGGGAG CGGACAGTGC TAGCTGGGCA GTGCTACATC 
CAGTTCCTCT CCCAGCCCAT CATCACCTTT GGCACAGCCA TGGCTGCCTT CTACCTCCCT 
GTCACAGTCA TGTGCACGCT CTACTGGCGC ATCTACCGGG AGACAGAGAA CCGAGCACGG 
GAGCTGGCAG CCCTTCAGGG CTCCGAGACG CCAGGCAAAG GGGGTGGCAG CAGCAGCAGC 
TCAGAGAGGT CTCAGCCAGG GGCTGAGGGC TCACCAGAGA CTCCTCCAGG CCGCTGCTGT 
CGCTGCTGCC GGGCCCCCAG GCTGCTGCAG GCCTACAGCT GGAAGGAAGA AGAGGAAGAG 
GACGAAGGCT CCATGGAGTC CCTCACATCC TCAGAGGGAG AGGAGCCTGG CTCCGAAGTG 
GTGATCAAGA TGCCAATGGT GGACCCCGAG GCACAGGCCC CCACCAAGCA GCCCCCACGG 
AGCTCCCCAA ATACAGTCAA GAGGCCGACT AAGAAAGGGC GTGATCGAGC TGGCAAGGGC 
CAGAAGCCCC GTGGAAAGGA GCAGCTGGCC AAGCGGAAGA CCTTCTCGCT GGTCAAGGAG 
AAGAAGGCGG CTCGGACCCT GAGTGCCATC CTCCTGGCCT TCATCCTCAC CTGGACACCG 
TACAACATCA TGGTGCTGGT GTCCACCTTC TGCAAGGACT GTGTTCCCGA GACCCTGTGG 
GAGCTGGGCT ACTGGCTGTG CTACGTCAAC AGCACCATCA ACCCCATGTG CTACGCACTC 
TGCAACAAAG CCTTCCGGGA CACCTTTCGC CTGCTGCTGC TTTGCCGCTG GGACAAGAGA 
CGCTGGCGCA AGATCCCCAA GCGCCCTGGC TCCGTGCACC GCACTCCCTC CCGCCAATGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHRM1 ( XM_011544742.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011543044.1 Homo sapiens cholinergic receptor, muscarinic 1 (CHRM1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu25587
Clone ID Related Accession (Same CDS sequence)	NM_000738.2 , NM_000738.3 , XM_011544742.1 , XM_011544742.2
Accession Version	XM_011544742.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1383bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	muscarinic acetylcholine receptor M1 isoform X1
Comment

ATGAACACTT CAGCCCCACC TGCTGTCAGC CCCAACATCA CCGTCCTGGC ACCAGGAAAG 
GGTCCCTGGC AAGTGGCCTT CATTGGGATC ACCACGGGCC TCCTGTCGCT AGCCACAGTG 
ACAGGCAACC TGCTGGTACT CATCTCTTTC AAGGTCAACA CGGAGCTCAA GACAGTCAAT 
AACTACTTCC TGCTGAGCCT GGCCTGTGCT GACCTCATCA TCGGTACCTT CTCCATGAAC 
CTCTATACCA CGTACCTGCT CATGGGCCAC TGGGCTCTGG GCACGCTGGC TTGTGACCTC 
TGGCTGGCCC TGGACTATGT GGCCAGCAAT GCCTCCGTCA TGAATCTGCT GCTCATCAGC 
TTTGACCGCT ACTTCTCCGT GACTCGGCCC CTGAGCTACC GTGCCAAGCG CACACCCCGC 
CGGGCAGCTC TGATGATCGG CCTGGCCTGG CTGGTTTCCT TTGTGCTCTG GGCCCCAGCC 
ATCCTCTTCT GGCAGTACCT GGTAGGGGAG CGGACAGTGC TAGCTGGGCA GTGCTACATC 
CAGTTCCTCT CCCAGCCCAT CATCACCTTT GGCACAGCCA TGGCTGCCTT CTACCTCCCT 
GTCACAGTCA TGTGCACGCT CTACTGGCGC ATCTACCGGG AGACAGAGAA CCGAGCACGG 
GAGCTGGCAG CCCTTCAGGG CTCCGAGACG CCAGGCAAAG GGGGTGGCAG CAGCAGCAGC 
TCAGAGAGGT CTCAGCCAGG GGCTGAGGGC TCACCAGAGA CTCCTCCAGG CCGCTGCTGT 
CGCTGCTGCC GGGCCCCCAG GCTGCTGCAG GCCTACAGCT GGAAGGAAGA AGAGGAAGAG 
GACGAAGGCT CCATGGAGTC CCTCACATCC TCAGAGGGAG AGGAGCCTGG CTCCGAAGTG 
GTGATCAAGA TGCCAATGGT GGACCCCGAG GCACAGGCCC CCACCAAGCA GCCCCCACGG 
AGCTCCCCAA ATACAGTCAA GAGGCCGACT AAGAAAGGGC GTGATCGAGC TGGCAAGGGC 
CAGAAGCCCC GTGGAAAGGA GCAGCTGGCC AAGCGGAAGA CCTTCTCGCT GGTCAAGGAG 
AAGAAGGCGG CTCGGACCCT GAGTGCCATC CTCCTGGCCT TCATCCTCAC CTGGACACCG 
TACAACATCA TGGTGCTGGT GTCCACCTTC TGCAAGGACT GTGTTCCCGA GACCCTGTGG 
GAGCTGGGCT ACTGGCTGTG CTACGTCAAC AGCACCATCA ACCCCATGTG CTACGCACTC 
TGCAACAAAG CCTTCCGGGA CACCTTTCGC CTGCTGCTGC TTTGCCGCTG GGACAAGAGA 
CGCTGGCGCA AGATCCCCAA GCGCCCTGGC TCCGTGCACC GCACTCCCTC CCGCCAATGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_011543044.1

Translation

MNTSAPPAVSPNITVLAPGKGPWQVAFIGITTGLLSLATVTGNLLVLISFKVNTELKTVNNYFLLSLACADLIIGTFSMNLYTTYLLMGHWALGTLACDLWLALDYVASNASVMNLLLISFDRYFSVTRPLSYRAKRTPRRAALMIGLAWLVSFVLWAPAILFWQYLVGERTVLAGQCYIQFLSQPIITFGTAMAAFYLPVTVMCTLYWRIYRETENRARELAALQGSETPGKGGGSSSSSERSQPGAEGSPETPPGRCCRCCRAPRLLQAYSWKEEEEEDEGSMESLTSSEGEEPGSEVVIKMPMVDPEAQAPTKQPPRSSPNTVKRPTKKGRDRAGKGQKPRGKEQLAKRKTFSLVKEKKAARTLSAILLAFILTWTPYNIMVLVSTFCKDCVPETLWELGYWLCYVNSTINPMCYALCNKAFRDTFRLLLLCRWDKRRWRKIPKRPGSVHRTPSRQC

Target ORF information:

RefSeq Version	XM_011544742.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens cholinergic receptor, muscarinic 1 (CHRM1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011544742.1

ORF Insert Sequence:

ATGAACACTT CAGCCCCACC TGCTGTCAGC CCCAACATCA CCGTCCTGGC ACCAGGAAAG 
GGTCCCTGGC AAGTGGCCTT CATTGGGATC ACCACGGGCC TCCTGTCGCT AGCCACAGTG 
ACAGGCAACC TGCTGGTACT CATCTCTTTC AAGGTCAACA CGGAGCTCAA GACAGTCAAT 
AACTACTTCC TGCTGAGCCT GGCCTGTGCT GACCTCATCA TCGGTACCTT CTCCATGAAC 
CTCTATACCA CGTACCTGCT CATGGGCCAC TGGGCTCTGG GCACGCTGGC TTGTGACCTC 
TGGCTGGCCC TGGACTATGT GGCCAGCAAT GCCTCCGTCA TGAATCTGCT GCTCATCAGC 
TTTGACCGCT ACTTCTCCGT GACTCGGCCC CTGAGCTACC GTGCCAAGCG CACACCCCGC 
CGGGCAGCTC TGATGATCGG CCTGGCCTGG CTGGTTTCCT TTGTGCTCTG GGCCCCAGCC 
ATCCTCTTCT GGCAGTACCT GGTAGGGGAG CGGACAGTGC TAGCTGGGCA GTGCTACATC 
CAGTTCCTCT CCCAGCCCAT CATCACCTTT GGCACAGCCA TGGCTGCCTT CTACCTCCCT 
GTCACAGTCA TGTGCACGCT CTACTGGCGC ATCTACCGGG AGACAGAGAA CCGAGCACGG 
GAGCTGGCAG CCCTTCAGGG CTCCGAGACG CCAGGCAAAG GGGGTGGCAG CAGCAGCAGC 
TCAGAGAGGT CTCAGCCAGG GGCTGAGGGC TCACCAGAGA CTCCTCCAGG CCGCTGCTGT 
CGCTGCTGCC GGGCCCCCAG GCTGCTGCAG GCCTACAGCT GGAAGGAAGA AGAGGAAGAG 
GACGAAGGCT CCATGGAGTC CCTCACATCC TCAGAGGGAG AGGAGCCTGG CTCCGAAGTG 
GTGATCAAGA TGCCAATGGT GGACCCCGAG GCACAGGCCC CCACCAAGCA GCCCCCACGG 
AGCTCCCCAA ATACAGTCAA GAGGCCGACT AAGAAAGGGC GTGATCGAGC TGGCAAGGGC 
CAGAAGCCCC GTGGAAAGGA GCAGCTGGCC AAGCGGAAGA CCTTCTCGCT GGTCAAGGAG 
AAGAAGGCGG CTCGGACCCT GAGTGCCATC CTCCTGGCCT TCATCCTCAC CTGGACACCG 
TACAACATCA TGGTGCTGGT GTCCACCTTC TGCAAGGACT GTGTTCCCGA GACCCTGTGG 
GAGCTGGGCT ACTGGCTGTG CTACGTCAAC AGCACCATCA ACCCCATGTG CTACGCACTC 
TGCAACAAAG CCTTCCGGGA CACCTTTCGC CTGCTGCTGC TTTGCCGCTG GGACAAGAGA 
CGCTGGCGCA AGATCCCCAA GCGCCCTGGC TCCGTGCACC GCACTCCCTC CCGCCAATGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHRM1 ( XM_011544742.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011543044.1 Homo sapiens cholinergic receptor muscarinic 1 (CHRM1), transcript variant X1, mRNA.

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CloneID	OHu25587
Clone ID Related Accession (Same CDS sequence)	NM_000738.2 , NM_000738.3 , XM_011544742.1 , XM_011544742.2
Accession Version	XM_011544742.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1383bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	muscarinic acetylcholine receptor M1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011544742.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACACTT CAGCCCCACC TGCTGTCAGC CCCAACATCA CCGTCCTGGC ACCAGGAAAG 
GGTCCCTGGC AAGTGGCCTT CATTGGGATC ACCACGGGCC TCCTGTCGCT AGCCACAGTG 
ACAGGCAACC TGCTGGTACT CATCTCTTTC AAGGTCAACA CGGAGCTCAA GACAGTCAAT 
AACTACTTCC TGCTGAGCCT GGCCTGTGCT GACCTCATCA TCGGTACCTT CTCCATGAAC 
CTCTATACCA CGTACCTGCT CATGGGCCAC TGGGCTCTGG GCACGCTGGC TTGTGACCTC 
TGGCTGGCCC TGGACTATGT GGCCAGCAAT GCCTCCGTCA TGAATCTGCT GCTCATCAGC 
TTTGACCGCT ACTTCTCCGT GACTCGGCCC CTGAGCTACC GTGCCAAGCG CACACCCCGC 
CGGGCAGCTC TGATGATCGG CCTGGCCTGG CTGGTTTCCT TTGTGCTCTG GGCCCCAGCC 
ATCCTCTTCT GGCAGTACCT GGTAGGGGAG CGGACAGTGC TAGCTGGGCA GTGCTACATC 
CAGTTCCTCT CCCAGCCCAT CATCACCTTT GGCACAGCCA TGGCTGCCTT CTACCTCCCT 
GTCACAGTCA TGTGCACGCT CTACTGGCGC ATCTACCGGG AGACAGAGAA CCGAGCACGG 
GAGCTGGCAG CCCTTCAGGG CTCCGAGACG CCAGGCAAAG GGGGTGGCAG CAGCAGCAGC 
TCAGAGAGGT CTCAGCCAGG GGCTGAGGGC TCACCAGAGA CTCCTCCAGG CCGCTGCTGT 
CGCTGCTGCC GGGCCCCCAG GCTGCTGCAG GCCTACAGCT GGAAGGAAGA AGAGGAAGAG 
GACGAAGGCT CCATGGAGTC CCTCACATCC TCAGAGGGAG AGGAGCCTGG CTCCGAAGTG 
GTGATCAAGA TGCCAATGGT GGACCCCGAG GCACAGGCCC CCACCAAGCA GCCCCCACGG 
AGCTCCCCAA ATACAGTCAA GAGGCCGACT AAGAAAGGGC GTGATCGAGC TGGCAAGGGC 
CAGAAGCCCC GTGGAAAGGA GCAGCTGGCC AAGCGGAAGA CCTTCTCGCT GGTCAAGGAG 
AAGAAGGCGG CTCGGACCCT GAGTGCCATC CTCCTGGCCT TCATCCTCAC CTGGACACCG 
TACAACATCA TGGTGCTGGT GTCCACCTTC TGCAAGGACT GTGTTCCCGA GACCCTGTGG 
GAGCTGGGCT ACTGGCTGTG CTACGTCAAC AGCACCATCA ACCCCATGTG CTACGCACTC 
TGCAACAAAG CCTTCCGGGA CACCTTTCGC CTGCTGCTGC TTTGCCGCTG GGACAAGAGA 
CGCTGGCGCA AGATCCCCAA GCGCCCTGGC TCCGTGCACC GCACTCCCTC CCGCCAATGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011543044.1
CDS	103..1485
Translation	MNTSAPPAVSPNITVLAPGKGPWQVAFIGITTGLLSLATVTGNLLVLISFKVNTELKTVNNYFLLSLACADLIIGTFSMNLYTTYLLMGHWALGTLACDLWLALDYVASNASVMNLLLISFDRYFSVTRPLSYRAKRTPRRAALMIGLAWLVSFVLWAPAILFWQYLVGERTVLAGQCYIQFLSQPIITFGTAMAAFYLPVTVMCTLYWRIYRETENRARELAALQGSETPGKGGGSSSSSERSQPGAEGSPETPPGRCCRCCRAPRLLQAYSWKEEEEEDEGSMESLTSSEGEEPGSEVVIKMPMVDPEAQAPTKQPPRSSPNTVKRPTKKGRDRAGKGQKPRGKEQLAKRKTFSLVKEKKAARTLSAILLAFILTWTPYNIMVLVSTFCKDCVPETLWELGYWLCYVNSTINPMCYALCNKAFRDTFRLLLLCRWDKRRWRKIPKRPGSVHRTPSRQC

Target ORF information:

RefSeq Version	XM_011544742.2
Organism	Homo sapiens(human)
Definition	Homo sapiens cholinergic receptor muscarinic 1 (CHRM1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011544742.2

ORF Insert Sequence:

ATGAACACTT CAGCCCCACC TGCTGTCAGC CCCAACATCA CCGTCCTGGC ACCAGGAAAG 
GGTCCCTGGC AAGTGGCCTT CATTGGGATC ACCACGGGCC TCCTGTCGCT AGCCACAGTG 
ACAGGCAACC TGCTGGTACT CATCTCTTTC AAGGTCAACA CGGAGCTCAA GACAGTCAAT 
AACTACTTCC TGCTGAGCCT GGCCTGTGCT GACCTCATCA TCGGTACCTT CTCCATGAAC 
CTCTATACCA CGTACCTGCT CATGGGCCAC TGGGCTCTGG GCACGCTGGC TTGTGACCTC 
TGGCTGGCCC TGGACTATGT GGCCAGCAAT GCCTCCGTCA TGAATCTGCT GCTCATCAGC 
TTTGACCGCT ACTTCTCCGT GACTCGGCCC CTGAGCTACC GTGCCAAGCG CACACCCCGC 
CGGGCAGCTC TGATGATCGG CCTGGCCTGG CTGGTTTCCT TTGTGCTCTG GGCCCCAGCC 
ATCCTCTTCT GGCAGTACCT GGTAGGGGAG CGGACAGTGC TAGCTGGGCA GTGCTACATC 
CAGTTCCTCT CCCAGCCCAT CATCACCTTT GGCACAGCCA TGGCTGCCTT CTACCTCCCT 
GTCACAGTCA TGTGCACGCT CTACTGGCGC ATCTACCGGG AGACAGAGAA CCGAGCACGG 
GAGCTGGCAG CCCTTCAGGG CTCCGAGACG CCAGGCAAAG GGGGTGGCAG CAGCAGCAGC 
TCAGAGAGGT CTCAGCCAGG GGCTGAGGGC TCACCAGAGA CTCCTCCAGG CCGCTGCTGT 
CGCTGCTGCC GGGCCCCCAG GCTGCTGCAG GCCTACAGCT GGAAGGAAGA AGAGGAAGAG 
GACGAAGGCT CCATGGAGTC CCTCACATCC TCAGAGGGAG AGGAGCCTGG CTCCGAAGTG 
GTGATCAAGA TGCCAATGGT GGACCCCGAG GCACAGGCCC CCACCAAGCA GCCCCCACGG 
AGCTCCCCAA ATACAGTCAA GAGGCCGACT AAGAAAGGGC GTGATCGAGC TGGCAAGGGC 
CAGAAGCCCC GTGGAAAGGA GCAGCTGGCC AAGCGGAAGA CCTTCTCGCT GGTCAAGGAG 
AAGAAGGCGG CTCGGACCCT GAGTGCCATC CTCCTGGCCT TCATCCTCAC CTGGACACCG 
TACAACATCA TGGTGCTGGT GTCCACCTTC TGCAAGGACT GTGTTCCCGA GACCCTGTGG 
GAGCTGGGCT ACTGGCTGTG CTACGTCAAC AGCACCATCA ACCCCATGTG CTACGCACTC 
TGCAACAAAG CCTTCCGGGA CACCTTTCGC CTGCTGCTGC TTTGCCGCTG GGACAAGAGA 
CGCTGGCGCA AGATCCCCAA GCGCCCTGGC TCCGTGCACC GCACTCCCTC CCGCCAATGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evidence of a M
Journal of receptor and signal transduction research37(3)267-275(2017 Jun)
Baig AM,Ahmad HR

Receptor Species-dependent Desensitization Controls KCNQ1/KCNE1 K+ Channels as Downstream Effectors of Gq Protein-coupled Receptors.
The Journal of biological chemistry291(51)26410-26426(2016 Dec)
Kienitz MC,Vladimirova D,M?ller C,Pott L,Rinne A

Association of the Cholinergic Muscarinic M2 Receptor with Autonomic Nervous System Activity in Patients with Schizophrenia on High-Dose Antipsychotics.
Neuropsychobiology74(1)60-67(2016)
Miyauchi M,Kishida I,Suda A,Shiraishi Y,Hattori S,Fujibayashi M,Taguri M,Ishii C,Ishii N,Moritani T,Hirayasu Y

Enhancing the Reliability of GPCR Models by Accounting for Flexibility of Their Pro-Containing Helices: the Case of the Human mAChR1 Receptor.
Molecular informatics34(4)216-27(2015 Apr)
Pedretti A,Mazzolari A,Ricci C,Vistoli G

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CHRM1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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