SUPT16H cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	SUPT16H
Entrez Gene ID	11198
Full Name	SPT16 homolog, facilitates chromatin remodeling subunit
Synonyms	CDC68,FACTP140,SPT16,SPT16/CDC68
General protein information	Preferred Names SPT16 homolog, facilitates chromatin remodeling subunit Names FACT complex subunit SPT16 FACT 140 kDa subunit chromatin-specific transcription elongation factor 140 kDa subunit facilitates chromatin remodeling 140 kDa subunit facilitates chromatin transcription complex subunit SPT16 hSPT16 suppressor of Ty 16 homolog
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 14 Map Location: 14q11.2
Summary	Transcription of protein-coding genes can be reconstituted on naked DNA with only the general transcription factors and RNA polymerase II. However, this minimal system cannot transcribe DNA packaged into chromatin, indicating that accessory factors may facilitate access to DNA. One such factor, FACT (facilitates chromatin transcription), interacts specifically with histones H2A/H2B to effect nucleosome disassembly and transcription elongation. FACT is composed of an 80 kDa subunit and a 140 kDa subunit; this gene encodes the 140 kDa subunit. [provided by RefSeq, Feb 2009].
Disorder MIM:	605012

mRNA and Protein(s)

mRNA	Protein	Name
NM_007192.3	NP_009123.1	FACT complex subunit SPT16
XM_011536381.2	XP_011534683.1	FACT complex subunit SPT16 isoform X1
NM_007192.4	NP_009123.1	FACT complex subunit SPT16

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Supt16	NP_291096.2
Kluyveromyces lactis	KLLA0F09889g	XP_455530.1
Eremothecium gossypii	AGOS_AER360C	NP_985216.2
Schizosaccharomyces pombe (fission yeast)	spt16	NP_596526.1
Oryza sativa (rice)	Os04g0321600	NP_001052463.1
Pan troglodytes (chimpanzee)	SUPT16H	XP_528703.2
Rattus norvegicus (Norway rat)	Supt16h	NP_001100731.1
Danio rerio (zebrafish)	supt16h	NP_001091053.1
Bos taurus (cattle)	SUPT16H	NP_001179321.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006817	XP_308929.4
Neurospora crassa	NCU01164	XP_961530.2
Arabidopsis thaliana (thale cress)	SPT16	NP_192809.1
Homo sapiens (human)	SUPT16H	NP_009123.1
Canis lupus familiaris (dog)	SUPT16H	XP_851566.1
Saccharomyces cerevisiae (baker's yeast)	SPT16	NP_011308.1
Drosophila melanogaster (fruit fly)	dre4	NP_728686.2
Caenorhabditis elegans (roundworm)	F55A3.3	NP_492821.1
Magnaporthe oryzae (rice blast fungus)	MGG_07316	XP_003715548.1

Gene Ontology
Bibliography

Related articles in PubMed

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

present observation and published data suggest that phenotype present in patients with duplication of 14q11.2 region, encompassing the SUPT16H and CHD8 genes, resemble in some extend features described in cases carrying microdeletion of that genomic region
Title: Novel 14q11.2 microduplication including the CHD8 and SUPT16H genes associated with developmental delay.

FACT is required for TOP1 binding to H3K4me3 at non-B DNA containing chromatin for the site-specific cleavage.
Title: Chromatin remodeller SMARCA4 recruits topoisomerase 1 and suppresses transcription-associated genomic instability.

In the absence of FACT complex, SSRP1 and SPT16 mRNAs are unstable and inefficiently translated, making reactivation of FACT function unlikely in normal cells.
Title: Complex mutual regulation of facilitates chromatin transcription (FACT) subunits on both mRNA and protein levels in human cells.

SUPT16H and RNF40 are required for proper DNA end resection and timely DNA repair after double-strand breaks.
Title: The H2B ubiquitin ligase RNF40 cooperates with SUPT16H to induce dynamic changes in chromatin structure during DNA double-strand break repair.

Data establish FACT as the major regulator involved in H2AX exchange process that is modulated by H2AX phosphorylation and Spt16 ADP-ribosylation.
Title: FACT-mediated exchange of histone variant H2AX regulated by phosphorylation of H2AX and ADP-ribosylation of Spt16.

The following SUPT16H gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SUPT16H cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu64837	XM_011536381.2 Latest version!	Homo sapiens SPT16 homolog, facilitates chromatin remodeling subunit (SUPT16H), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$475.30 $679.00
OHu22815	NM_007192.4 Latest version!	Homo sapiens SPT16 homolog, facilitates chromatin remodeling subunit (SUPT16H), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
View Old Accession Versions >>
OHu22815	NM_007192.3	Homo sapiens SPT16 homolog, facilitates chromatin remodeling subunit (SUPT16H), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SUPT16H ( XM_011536381.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011534683.1 Homo sapiens SPT16 homolog, facilitates chromatin remodeling subunit (SUPT16H), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu64837
Clone ID Related Accession (Same CDS sequence)	XM_011536381.2
Accession Version	XM_011536381.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2949bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	FACT complex subunit SPT16 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011536381.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCTTTT GTGATGACAA AATCATCTTT ATGGCCAGCA AGAAAAAAGT GGAGTTCTTG 
AAACAGATTG CCAACACTAA GGGCAATGAG AATGCTAATG GAGCCCCTGC CATCACACTG 
CTAATACGAG AAAAGAATGA AAGTAATAAG AGTAGCTTTG ACAAAATGAT TGAAGCCATT 
AAAGAAAGCA AGAATGGCAA GAAGATTGGA GTGTTCAGCA AAGACAAATT CCCTGGAGAG 
TTCATGAAGA GCTGGAATGA CTGCCTCAAC AAAGAAGGCT TTGACAAAAT AGATATCAGT 
GCAGTTGTGG CATATACCAT CGCTGTAAAG GAGGATGGGG AGCTCAACCT AATGAAGAAA 
GCAGCCAGCA TCACTTCTGA AGTCTTCAAC AAATTCTTCA AGGAAAGAGT CATGGAAATA 
GTTGATGCAG ATGAGAAAGT TCGACACAGC AAACTGGCTG AGTCTGTGGA AAAGGCCATT 
GAAGAGAAAA AATACCTTGC TGGGGCAGAC CCTTCTACTG TGGAAATGTG TTACCCTCCT 
ATCATTCAGA GTGGTGGCAA CTATAATCTC AAGTTCAGTG TGGTGAGTGA CAAGAATCAT 
ATGCACTTTG GGGCTATCAC TTGTGCCATG GGTATTCGCT TCAAGTCTTA CTGCTCCAAC 
CTTGTTCGCA CTTTGATGGT TGATCCTTCT CAAGAAGTTC AAGAAAATTA TAACTTTTTG 
CTCCAGCTTC AAGAGGAGCT GCTGAAGGAA TTAAGACATG GTGTGAAGAT ATGTGACGTG 
TATAACGCTG TCATGGACGT GGTTAAAAAG CAGAAGCCAG AACTGCTGAA CAAAATTACC 
AAAAACCTAG GGTTTGGGAT GGGAATTGAA TTCCGTGAAG GCTCCCTAGT AATCAATAGC 
AAAAATCAAT ACAAACTGAA GAAAGGAATG GTTTTCAGCA TCAATTTAGG ATTCTCAGAC 
CTGACTAACA AGGAGGGGAA AAAGCCAGAA GAGAAAACCT ATGCCCTGTT CATTGGTGAC 
ACAGTGCTTG TGGATGAGGA TGGCCCAGCT ACTGTTCTCA CTTCTGTGAA GAAGAAAGTG 
AAGAATGTGG GGATTTTCCT AAAGAATGAA GATGAGGAAG AAGAGGAGGA GGAGAAAGAT 
GAGGCAGAGG ACCTTTTGGG AAGAGGTTCT CGGGCAGCAT TACTTACAGA AAGAACAAGA 
AATGAAATGA CTGCAGAAGA GAAGCGAAGA GCACATCAGA AAGAACTAGC GGCTCAACTC 
AATGAAGAAG CAAAGAGGCG ATTGACTGAA CAAAAGGGAG AACAGCAGAT TCAGAAAGCT 
CGCAAGTCTA ATGTGTCCTA TAAAAACCCA TCTCTGATGC CTAAGGAACC ACATATTCGG 
GAAATGAAGA TCTACATCGA TAAGAAATAT GAGACTGTAA TAATGCCCGT GTTTGGCATT 
GCAACACCGT TTCACATTGC CACAATCAAG AATATAAGTA TGTCCGTGGA AGGAGATTAT 
ACTTACTTGC GAATCAACTT TTATTGCCCA GGCAGTGCTC TGGGCAGGAA TGAAGGCAAC 
ATCTTTCCTA ACCCTGAAGC GACTTTTGTC AAGGAAATTA CATACCGAGC ATCAAATATT 
AAGGCACCCG GAGAACAGAC AGTACCAGCC TTGAACCTTC AGAATGCTTT CCGAATTATT 
AAAGAAGTAC AGAAACGTTA TAAAACTCGA GAAGCTGAAG AGAAAGAGAA GGAGGGGATT 
GTAAAACAAG ACTCACTGGT GATCAATCTA AACCGGAGTA ATCCGAAACT GAAAGATCTA 
TACATTCGCC CAAATATTGC CCAAAAGAGG ATGCAAGGCT CACTGGAGGC CCATGTCAAT 
GGCTTCCGCT TCACATCTGT TCGAGGAGAC AAAGTGGATA TTTTGTACAA TAATATTAAG 
CATGCTTTGT TCCAGCCCTG TGATGGAGAA ATGATTATTG TCTTGCACTT TCACCTCAAG 
AATGCCATCA TGTTTGGGAA GAAGCGGCAC ACGGATGTGC AGTTCTACAC AGAAGTGGGA 
GAGATAACCA CGGACTTGGG GAAACATCAG CATATGCATG ACCGAGATGA CCTCTATGCT 
GAGCAGATGG AACGAGAAAT GAGGCACAAA CTGAAAACAG CCTTTAAAAA TTTCATTGAG 
AAAGTAGAGG CTCTAACTAA GGAGGAACTG GAATTTGAAG TGCCTTTTAG GGACTTGGGA 
TTTAACGGAG CTCCCTATAG GAGTACCTGC CTCCTTCAGC CCACTAGTAG TGCGCTGGTA 
AATGCTACGG AATGGCCACC TTTTGTGGTG ACATTGGATG AGGTAGAGCT GATCCACTTT 
GAGCGGGTCC AGTTTCACCT GAAGAACTTT GATATGGTAA TCGTCTACAA GGACTACAGC 
AAGAAAGTGA CCATGATCAA CGCCATTCCT GTAGCCTCTC TTGACCCCAT CAAGGAATGG 
TTGAATTCCT GCGACCTGAA ATACACAGAA GGAGTACAGT CCCTCAACTG GACTAAAATC 
ATGAAGACCA TTGTTGATGA CCCTGAGGGC TTCTTCGAAC AAGGTGGCTG GTCTTTCCTG 
GAGCCTGAGG GTGAGGGGAG TGATGCTGAA GAAGGGGATT CAGAGTCTGA AATTGAAGAT 
GAGACTTTTA ATCCTTCAGA AGATGACTAT GAAGAGGAAG AGGAGGACAG TGATGAAGAT 
TATTCATCAG AAGCAGAAGA GTCAGACTAT TCTAAGGAGT CATTGGGTAG TGAAGAAGAG 
AGTGGAAAGG ATTGGGATGA ACTGGAGGAA GAAGCCCGAA AAGCGGACCG AGAAAGTCGT 
TACGAGGAAG AAGAAGAACA AAGTCGAAGT ATGAGCCGGA AGAGGAAGGC ATCTGTGCAC 
AGTTCGGGCC GTGGCTCTAA CCGTGGTTCC AGACACAGCT CTGCACCCCC CAAGAAAAAG 
AGGAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011534683.1
CDS	537..3485
Translation	MVFCDDKIIFMASKKKVEFLKQIANTKGNENANGAPAITLLIREKNESNKSSFDKMIEAIKESKNGKKIGVFSKDKFPGEFMKSWNDCLNKEGFDKIDISAVVAYTIAVKEDGELNLMKKAASITSEVFNKFFKERVMEIVDADEKVRHSKLAESVEKAIEEKKYLAGADPSTVEMCYPPIIQSGGNYNLKFSVVSDKNHMHFGAITCAMGIRFKSYCSNLVRTLMVDPSQEVQENYNFLLQLQEELLKELRHGVKICDVYNAVMDVVKKQKPELLNKITKNLGFGMGIEFREGSLVINSKNQYKLKKGMVFSINLGFSDLTNKEGKKPEEKTYALFIGDTVLVDEDGPATVLTSVKKKVKNVGIFLKNEDEEEEEEEKDEAEDLLGRGSRAALLTERTRNEMTAEEKRRAHQKELAAQLNEEAKRRLTEQKGEQQIQKARKSNVSYKNPSLMPKEPHIREMKIYIDKKYETVIMPVFGIATPFHIATIKNISMSVEGDYTYLRINFYCPGSALGRNEGNIFPNPEATFVKEITYRASNIKAPGEQTVPALNLQNAFRIIKEVQKRYKTREAEEKEKEGIVKQDSLVINLNRSNPKLKDLYIRPNIAQKRMQGSLEAHVNGFRFTSVRGDKVDILYNNIKHALFQPCDGEMIIVLHFHLKNAIMFGKKRHTDVQFYTEVGEITTDLGKHQHMHDRDDLYAEQMEREMRHKLKTAFKNFIEKVEALTKEELEFEVPFRDLGFNGAPYRSTCLLQPTSSALVNATEWPPFVVTLDEVELIHFERVQFHLKNFDMVIVYKDYSKKVTMINAIPVASLDPIKEWLNSCDLKYTEGVQSLNWTKIMKTIVDDPEGFFEQGGWSFLEPEGEGSDAEEGDSESEIEDETFNPSEDDYEEEEEDSDEDYSSEAEESDYSKESLGSEEESGKDWDELEEEARKADRESRYEEEEEQSRSMSRKRKASVHSSGRGSNRGSRHSSAPPKKKRK

Target ORF information:

RefSeq Version	XM_011536381.2
Organism	Homo sapiens(human)
Definition	Homo sapiens SPT16 homolog, facilitates chromatin remodeling subunit (SUPT16H), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011536381.2

ORF Insert Sequence:

ATGGTCTTTT GTGATGACAA AATCATCTTT ATGGCCAGCA AGAAAAAAGT GGAGTTCTTG 
AAACAGATTG CCAACACTAA GGGCAATGAG AATGCTAATG GAGCCCCTGC CATCACACTG 
CTAATACGAG AAAAGAATGA AAGTAATAAG AGTAGCTTTG ACAAAATGAT TGAAGCCATT 
AAAGAAAGCA AGAATGGCAA GAAGATTGGA GTGTTCAGCA AAGACAAATT CCCTGGAGAG 
TTCATGAAGA GCTGGAATGA CTGCCTCAAC AAAGAAGGCT TTGACAAAAT AGATATCAGT 
GCAGTTGTGG CATATACCAT CGCTGTAAAG GAGGATGGGG AGCTCAACCT AATGAAGAAA 
GCAGCCAGCA TCACTTCTGA AGTCTTCAAC AAATTCTTCA AGGAAAGAGT CATGGAAATA 
GTTGATGCAG ATGAGAAAGT TCGACACAGC AAACTGGCTG AGTCTGTGGA AAAGGCCATT 
GAAGAGAAAA AATACCTTGC TGGGGCAGAC CCTTCTACTG TGGAAATGTG TTACCCTCCT 
ATCATTCAGA GTGGTGGCAA CTATAATCTC AAGTTCAGTG TGGTGAGTGA CAAGAATCAT 
ATGCACTTTG GGGCTATCAC TTGTGCCATG GGTATTCGCT TCAAGTCTTA CTGCTCCAAC 
CTTGTTCGCA CTTTGATGGT TGATCCTTCT CAAGAAGTTC AAGAAAATTA TAACTTTTTG 
CTCCAGCTTC AAGAGGAGCT GCTGAAGGAA TTAAGACATG GTGTGAAGAT ATGTGACGTG 
TATAACGCTG TCATGGACGT GGTTAAAAAG CAGAAGCCAG AACTGCTGAA CAAAATTACC 
AAAAACCTAG GGTTTGGGAT GGGAATTGAA TTCCGTGAAG GCTCCCTAGT AATCAATAGC 
AAAAATCAAT ACAAACTGAA GAAAGGAATG GTTTTCAGCA TCAATTTAGG ATTCTCAGAC 
CTGACTAACA AGGAGGGGAA AAAGCCAGAA GAGAAAACCT ATGCCCTGTT CATTGGTGAC 
ACAGTGCTTG TGGATGAGGA TGGCCCAGCT ACTGTTCTCA CTTCTGTGAA GAAGAAAGTG 
AAGAATGTGG GGATTTTCCT AAAGAATGAA GATGAGGAAG AAGAGGAGGA GGAGAAAGAT 
GAGGCAGAGG ACCTTTTGGG AAGAGGTTCT CGGGCAGCAT TACTTACAGA AAGAACAAGA 
AATGAAATGA CTGCAGAAGA GAAGCGAAGA GCACATCAGA AAGAACTAGC GGCTCAACTC 
AATGAAGAAG CAAAGAGGCG ATTGACTGAA CAAAAGGGAG AACAGCAGAT TCAGAAAGCT 
CGCAAGTCTA ATGTGTCCTA TAAAAACCCA TCTCTGATGC CTAAGGAACC ACATATTCGG 
GAAATGAAGA TCTACATCGA TAAGAAATAT GAGACTGTAA TAATGCCCGT GTTTGGCATT 
GCAACACCGT TTCACATTGC CACAATCAAG AATATAAGTA TGTCCGTGGA AGGAGATTAT 
ACTTACTTGC GAATCAACTT TTATTGCCCA GGCAGTGCTC TGGGCAGGAA TGAAGGCAAC 
ATCTTTCCTA ACCCTGAAGC GACTTTTGTC AAGGAAATTA CATACCGAGC ATCAAATATT 
AAGGCACCCG GAGAACAGAC AGTACCAGCC TTGAACCTTC AGAATGCTTT CCGAATTATT 
AAAGAAGTAC AGAAACGTTA TAAAACTCGA GAAGCTGAAG AGAAAGAGAA GGAGGGGATT 
GTAAAACAAG ACTCACTGGT GATCAATCTA AACCGGAGTA ATCCGAAACT GAAAGATCTA 
TACATTCGCC CAAATATTGC CCAAAAGAGG ATGCAAGGCT CACTGGAGGC CCATGTCAAT 
GGCTTCCGCT TCACATCTGT TCGAGGAGAC AAAGTGGATA TTTTGTACAA TAATATTAAG 
CATGCTTTGT TCCAGCCCTG TGATGGAGAA ATGATTATTG TCTTGCACTT TCACCTCAAG 
AATGCCATCA TGTTTGGGAA GAAGCGGCAC ACGGATGTGC AGTTCTACAC AGAAGTGGGA 
GAGATAACCA CGGACTTGGG GAAACATCAG CATATGCATG ACCGAGATGA CCTCTATGCT 
GAGCAGATGG AACGAGAAAT GAGGCACAAA CTGAAAACAG CCTTTAAAAA TTTCATTGAG 
AAAGTAGAGG CTCTAACTAA GGAGGAACTG GAATTTGAAG TGCCTTTTAG GGACTTGGGA 
TTTAACGGAG CTCCCTATAG GAGTACCTGC CTCCTTCAGC CCACTAGTAG TGCGCTGGTA 
AATGCTACGG AATGGCCACC TTTTGTGGTG ACATTGGATG AGGTAGAGCT GATCCACTTT 
GAGCGGGTCC AGTTTCACCT GAAGAACTTT GATATGGTAA TCGTCTACAA GGACTACAGC 
AAGAAAGTGA CCATGATCAA CGCCATTCCT GTAGCCTCTC TTGACCCCAT CAAGGAATGG 
TTGAATTCCT GCGACCTGAA ATACACAGAA GGAGTACAGT CCCTCAACTG GACTAAAATC 
ATGAAGACCA TTGTTGATGA CCCTGAGGGC TTCTTCGAAC AAGGTGGCTG GTCTTTCCTG 
GAGCCTGAGG GTGAGGGGAG TGATGCTGAA GAAGGGGATT CAGAGTCTGA AATTGAAGAT 
GAGACTTTTA ATCCTTCAGA AGATGACTAT GAAGAGGAAG AGGAGGACAG TGATGAAGAT 
TATTCATCAG AAGCAGAAGA GTCAGACTAT TCTAAGGAGT CATTGGGTAG TGAAGAAGAG 
AGTGGAAAGG ATTGGGATGA ACTGGAGGAA GAAGCCCGAA AAGCGGACCG AGAAAGTCGT 
TACGAGGAAG AAGAAGAACA AAGTCGAAGT ATGAGCCGGA AGAGGAAGGC ATCTGTGCAC 
AGTTCGGGCC GTGGCTCTAA CCGTGGTTCC AGACACAGCT CTGCACCCCC CAAGAAAAAG 
AGGAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SUPT16H ( NM_007192.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_009123.1 Homo sapiens SPT16 homolog, facilitates chromatin remodeling subunit (SUPT16H), mRNA.

Price & Availability↑

CloneID	OHu22815
Clone ID Related Accession (Same CDS sequence)	NM_007192.3 , NM_007192.4
Accession Version	NM_007192.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3144bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	FACT complex subunit SPT16
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK024072.1, AK001719.1, BC014046.2, BP872403.1 and AL135744.4. This sequence is a reference standard in the RefSeqGene project. On Feb 20, 2009 this sequence version replaced NM_007192.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.157934.1, SRR1660805.146067.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTGTGA CTCTGGACAA AGACGCTTAT TATCGGCGAG TGAAGAGACT GTACAGCAAT 
TGGCGGAAAG GAGAAGATGA GTATGCCAAC GTTGATGCCA TTGTTGTATC AGTGGGTGTT 
GATGAAGAAA TTGTTTATGC CAAATCAACT GCCTTACAGA CATGGCTCTT TGGTTATGAA 
CTAACTGATA CTATCATGGT CTTTTGTGAT GACAAAATCA TCTTTATGGC CAGCAAGAAA 
AAAGTGGAGT TCTTGAAACA GATTGCCAAC ACTAAGGGCA ATGAGAATGC TAATGGAGCC 
CCTGCCATCA CACTGCTAAT ACGAGAAAAG AATGAAAGTA ATAAGAGTAG CTTTGACAAA 
ATGATTGAAG CCATTAAAGA AAGCAAGAAT GGCAAGAAGA TTGGAGTGTT CAGCAAAGAC 
AAATTCCCTG GAGAGTTCAT GAAGAGCTGG AATGACTGCC TCAACAAAGA AGGCTTTGAC 
AAAATAGATA TCAGTGCAGT TGTGGCATAT ACCATCGCTG TAAAGGAGGA TGGGGAGCTC 
AACCTAATGA AGAAAGCAGC CAGCATCACT TCTGAAGTCT TCAACAAATT CTTCAAGGAA 
AGAGTCATGG AAATAGTTGA TGCAGATGAG AAAGTTCGAC ACAGCAAACT GGCTGAGTCT 
GTGGAAAAGG CCATTGAAGA GAAAAAATAC CTTGCTGGGG CAGACCCTTC TACTGTGGAA 
ATGTGTTACC CTCCTATCAT TCAGAGTGGT GGCAACTATA ATCTCAAGTT CAGTGTGGTG 
AGTGACAAGA ATCATATGCA CTTTGGGGCT ATCACTTGTG CCATGGGTAT TCGCTTCAAG 
TCTTACTGCT CCAACCTTGT TCGCACTTTG ATGGTTGATC CTTCTCAAGA AGTTCAAGAA 
AATTATAACT TTTTGCTCCA GCTTCAAGAG GAGCTGCTGA AGGAATTAAG ACATGGTGTG 
AAGATATGTG ACGTGTATAA CGCTGTCATG GACGTGGTTA AAAAGCAGAA GCCAGAACTG 
CTGAACAAAA TTACCAAAAA CCTAGGGTTT GGGATGGGAA TTGAATTCCG TGAAGGCTCC 
CTAGTAATCA ATAGCAAAAA TCAATACAAA CTGAAGAAAG GAATGGTTTT CAGCATCAAT 
TTAGGATTCT CAGACCTGAC TAACAAGGAG GGGAAAAAGC CAGAAGAGAA AACCTATGCC 
CTGTTCATTG GTGACACAGT GCTTGTGGAT GAGGATGGCC CAGCTACTGT TCTCACTTCT 
GTGAAGAAGA AAGTGAAGAA TGTGGGGATT TTCCTAAAGA ATGAAGATGA GGAAGAAGAG 
GAGGAGGAGA AAGATGAGGC AGAGGACCTT TTGGGAAGAG GTTCTCGGGC AGCATTACTT 
ACAGAAAGAA CAAGAAATGA AATGACTGCA GAAGAGAAGC GAAGAGCACA TCAGAAAGAA 
CTAGCGGCTC AACTCAATGA AGAAGCAAAG AGGCGATTGA CTGAACAAAA GGGAGAACAG 
CAGATTCAGA AAGCTCGCAA GTCTAATGTG TCCTATAAAA ACCCATCTCT GATGCCTAAG 
GAACCACATA TTCGGGAAAT GAAGATCTAC ATCGATAAGA AATATGAGAC TGTAATAATG 
CCCGTGTTTG GCATTGCAAC ACCGTTTCAC ATTGCCACAA TCAAGAATAT AAGTATGTCC 
GTGGAAGGAG ATTATACTTA CTTGCGAATC AACTTTTATT GCCCAGGCAG TGCTCTGGGC 
AGGAATGAAG GCAACATCTT TCCTAACCCT GAAGCGACTT TTGTCAAGGA AATTACATAC 
CGAGCATCAA ATATTAAGGC ACCCGGAGAA CAGACAGTAC CAGCCTTGAA CCTTCAGAAT 
GCTTTCCGAA TTATTAAAGA AGTACAGAAA CGTTATAAAA CTCGAGAAGC TGAAGAGAAA 
GAGAAGGAGG GGATTGTAAA ACAAGACTCA CTGGTGATCA ATCTAAACCG GAGTAATCCG 
AAACTGAAAG ATCTATACAT TCGCCCAAAT ATTGCCCAAA AGAGGATGCA AGGCTCACTG 
GAGGCCCATG TCAATGGCTT CCGCTTCACA TCTGTTCGAG GAGACAAAGT GGATATTTTG 
TACAATAATA TTAAGCATGC TTTGTTCCAG CCCTGTGATG GAGAAATGAT TATTGTCTTG 
CACTTTCACC TCAAGAATGC CATCATGTTT GGGAAGAAGC GGCACACGGA TGTGCAGTTC 
TACACAGAAG TGGGAGAGAT AACCACGGAC TTGGGGAAAC ATCAGCATAT GCATGACCGA 
GATGACCTCT ATGCTGAGCA GATGGAACGA GAAATGAGGC ACAAACTGAA AACAGCCTTT 
AAAAATTTCA TTGAGAAAGT AGAGGCTCTA ACTAAGGAGG AACTGGAATT TGAAGTGCCT 
TTTAGGGACT TGGGATTTAA CGGAGCTCCC TATAGGAGTA CCTGCCTCCT TCAGCCCACT 
AGTAGTGCGC TGGTAAATGC TACGGAATGG CCACCTTTTG TGGTGACATT GGATGAGGTA 
GAGCTGATCC ACTTTGAGCG GGTCCAGTTT CACCTGAAGA ACTTTGATAT GGTAATCGTC 
TACAAGGACT ACAGCAAGAA AGTGACCATG ATCAACGCCA TTCCTGTAGC CTCTCTTGAC 
CCCATCAAGG AATGGTTGAA TTCCTGCGAC CTGAAATACA CAGAAGGAGT ACAGTCCCTC 
AACTGGACTA AAATCATGAA GACCATTGTT GATGACCCTG AGGGCTTCTT CGAACAAGGT 
GGCTGGTCTT TCCTGGAGCC TGAGGGTGAG GGGAGTGATG CTGAAGAAGG GGATTCAGAG 
TCTGAAATTG AAGATGAGAC TTTTAATCCT TCAGAAGATG ACTATGAAGA GGAAGAGGAG 
GACAGTGATG AAGATTATTC ATCAGAAGCA GAAGAGTCAG ACTATTCTAA GGAGTCATTG 
GGTAGTGAAG AAGAGAGTGG AAAGGATTGG GATGAACTGG AGGAAGAAGC CCGAAAAGCG 
GACCGAGAAA GTCGTTACGA GGAAGAAGAA GAACAAAGTC GAAGTATGAG CCGGAAGAGG 
AAGGCATCTG TGCACAGTTC GGGCCGTGGC TCTAACCGTG GTTCCAGACA CAGCTCTGCA 
CCCCCCAAGA AAAAGAGGAA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_009123.1
CDS	340..3483
Translation	MAVTLDKDAYYRRVKRLYSNWRKGEDEYANVDAIVVSVGVDEEIVYAKSTALQTWLFGYELTDTIMVFCDDKIIFMASKKKVEFLKQIANTKGNENANGAPAITLLIREKNESNKSSFDKMIEAIKESKNGKKIGVFSKDKFPGEFMKSWNDCLNKEGFDKIDISAVVAYTIAVKEDGELNLMKKAASITSEVFNKFFKERVMEIVDADEKVRHSKLAESVEKAIEEKKYLAGADPSTVEMCYPPIIQSGGNYNLKFSVVSDKNHMHFGAITCAMGIRFKSYCSNLVRTLMVDPSQEVQENYNFLLQLQEELLKELRHGVKICDVYNAVMDVVKKQKPELLNKITKNLGFGMGIEFREGSLVINSKNQYKLKKGMVFSINLGFSDLTNKEGKKPEEKTYALFIGDTVLVDEDGPATVLTSVKKKVKNVGIFLKNEDEEEEEEEKDEAEDLLGRGSRAALLTERTRNEMTAEEKRRAHQKELAAQLNEEAKRRLTEQKGEQQIQKARKSNVSYKNPSLMPKEPHIREMKIYIDKKYETVIMPVFGIATPFHIATIKNISMSVEGDYTYLRINFYCPGSALGRNEGNIFPNPEATFVKEITYRASNIKAPGEQTVPALNLQNAFRIIKEVQKRYKTREAEEKEKEGIVKQDSLVINLNRSNPKLKDLYIRPNIAQKRMQGSLEAHVNGFRFTSVRGDKVDILYNNIKHALFQPCDGEMIIVLHFHLKNAIMFGKKRHTDVQFYTEVGEITTDLGKHQHMHDRDDLYAEQMEREMRHKLKTAFKNFIEKVEALTKEELEFEVPFRDLGFNGAPYRSTCLLQPTSSALVNATEWPPFVVTLDEVELIHFERVQFHLKNFDMVIVYKDYSKKVTMINAIPVASLDPIKEWLNSCDLKYTEGVQSLNWTKIMKTIVDDPEGFFEQGGWSFLEPEGEGSDAEEGDSESEIEDETFNPSEDDYEEEEEDSDEDYSSEAEESDYSKESLGSEEESGKDWDELEEEARKADRESRYEEEEEQSRSMSRKRKASVHSSGRGSNRGSRHSSAPPKKKRK

Target ORF information:

RefSeq Version	NM_007192.3
Organism	Homo sapiens(human)
Definition	Homo sapiens SPT16 homolog, facilitates chromatin remodeling subunit (SUPT16H), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007192.3

ORF Insert Sequence:

ATGGCTGTGA CTCTGGACAA AGACGCTTAT TATCGGCGAG TGAAGAGACT GTACAGCAAT 
TGGCGGAAAG GAGAAGATGA GTATGCCAAC GTTGATGCCA TTGTTGTATC AGTGGGTGTT 
GATGAAGAAA TTGTTTATGC CAAATCAACT GCCTTACAGA CATGGCTCTT TGGTTATGAA 
CTAACTGATA CTATCATGGT CTTTTGTGAT GACAAAATCA TCTTTATGGC CAGCAAGAAA 
AAAGTGGAGT TCTTGAAACA GATTGCCAAC ACTAAGGGCA ATGAGAATGC TAATGGAGCC 
CCTGCCATCA CACTGCTAAT ACGAGAAAAG AATGAAAGTA ATAAGAGTAG CTTTGACAAA 
ATGATTGAAG CCATTAAAGA AAGCAAGAAT GGCAAGAAGA TTGGAGTGTT CAGCAAAGAC 
AAATTCCCTG GAGAGTTCAT GAAGAGCTGG AATGACTGCC TCAACAAAGA AGGCTTTGAC 
AAAATAGATA TCAGTGCAGT TGTGGCATAT ACCATCGCTG TAAAGGAGGA TGGGGAGCTC 
AACCTAATGA AGAAAGCAGC CAGCATCACT TCTGAAGTCT TCAACAAATT CTTCAAGGAA 
AGAGTCATGG AAATAGTTGA TGCAGATGAG AAAGTTCGAC ACAGCAAACT GGCTGAGTCT 
GTGGAAAAGG CCATTGAAGA GAAAAAATAC CTTGCTGGGG CAGACCCTTC TACTGTGGAA 
ATGTGTTACC CTCCTATCAT TCAGAGTGGT GGCAACTATA ATCTCAAGTT CAGTGTGGTG 
AGTGACAAGA ATCATATGCA CTTTGGGGCT ATCACTTGTG CCATGGGTAT TCGCTTCAAG 
TCTTACTGCT CCAACCTTGT TCGCACTTTG ATGGTTGATC CTTCTCAAGA AGTTCAAGAA 
AATTATAACT TTTTGCTCCA GCTTCAAGAG GAGCTGCTGA AGGAATTAAG ACATGGTGTG 
AAGATATGTG ACGTGTATAA CGCTGTCATG GACGTGGTTA AAAAGCAGAA GCCAGAACTG 
CTGAACAAAA TTACCAAAAA CCTAGGGTTT GGGATGGGAA TTGAATTCCG TGAAGGCTCC 
CTAGTAATCA ATAGCAAAAA TCAATACAAA CTGAAGAAAG GAATGGTTTT CAGCATCAAT 
TTAGGATTCT CAGACCTGAC TAACAAGGAG GGGAAAAAGC CAGAAGAGAA AACCTATGCC 
CTGTTCATTG GTGACACAGT GCTTGTGGAT GAGGATGGCC CAGCTACTGT TCTCACTTCT 
GTGAAGAAGA AAGTGAAGAA TGTGGGGATT TTCCTAAAGA ATGAAGATGA GGAAGAAGAG 
GAGGAGGAGA AAGATGAGGC AGAGGACCTT TTGGGAAGAG GTTCTCGGGC AGCATTACTT 
ACAGAAAGAA CAAGAAATGA AATGACTGCA GAAGAGAAGC GAAGAGCACA TCAGAAAGAA 
CTAGCGGCTC AACTCAATGA AGAAGCAAAG AGGCGATTGA CTGAACAAAA GGGAGAACAG 
CAGATTCAGA AAGCTCGCAA GTCTAATGTG TCCTATAAAA ACCCATCTCT GATGCCTAAG 
GAACCACATA TTCGGGAAAT GAAGATCTAC ATCGATAAGA AATATGAGAC TGTAATAATG 
CCCGTGTTTG GCATTGCAAC ACCGTTTCAC ATTGCCACAA TCAAGAATAT AAGTATGTCC 
GTGGAAGGAG ATTATACTTA CTTGCGAATC AACTTTTATT GCCCAGGCAG TGCTCTGGGC 
AGGAATGAAG GCAACATCTT TCCTAACCCT GAAGCGACTT TTGTCAAGGA AATTACATAC 
CGAGCATCAA ATATTAAGGC ACCCGGAGAA CAGACAGTAC CAGCCTTGAA CCTTCAGAAT 
GCTTTCCGAA TTATTAAAGA AGTACAGAAA CGTTATAAAA CTCGAGAAGC TGAAGAGAAA 
GAGAAGGAGG GGATTGTAAA ACAAGACTCA CTGGTGATCA ATCTAAACCG GAGTAATCCG 
AAACTGAAAG ATCTATACAT TCGCCCAAAT ATTGCCCAAA AGAGGATGCA AGGCTCACTG 
GAGGCCCATG TCAATGGCTT CCGCTTCACA TCTGTTCGAG GAGACAAAGT GGATATTTTG 
TACAATAATA TTAAGCATGC TTTGTTCCAG CCCTGTGATG GAGAAATGAT TATTGTCTTG 
CACTTTCACC TCAAGAATGC CATCATGTTT GGGAAGAAGC GGCACACGGA TGTGCAGTTC 
TACACAGAAG TGGGAGAGAT AACCACGGAC TTGGGGAAAC ATCAGCATAT GCATGACCGA 
GATGACCTCT ATGCTGAGCA GATGGAACGA GAAATGAGGC ACAAACTGAA AACAGCCTTT 
AAAAATTTCA TTGAGAAAGT AGAGGCTCTA ACTAAGGAGG AACTGGAATT TGAAGTGCCT 
TTTAGGGACT TGGGATTTAA CGGAGCTCCC TATAGGAGTA CCTGCCTCCT TCAGCCCACT 
AGTAGTGCGC TGGTAAATGC TACGGAATGG CCACCTTTTG TGGTGACATT GGATGAGGTA 
GAGCTGATCC ACTTTGAGCG GGTCCAGTTT CACCTGAAGA ACTTTGATAT GGTAATCGTC 
TACAAGGACT ACAGCAAGAA AGTGACCATG ATCAACGCCA TTCCTGTAGC CTCTCTTGAC 
CCCATCAAGG AATGGTTGAA TTCCTGCGAC CTGAAATACA CAGAAGGAGT ACAGTCCCTC 
AACTGGACTA AAATCATGAA GACCATTGTT GATGACCCTG AGGGCTTCTT CGAACAAGGT 
GGCTGGTCTT TCCTGGAGCC TGAGGGTGAG GGGAGTGATG CTGAAGAAGG GGATTCAGAG 
TCTGAAATTG AAGATGAGAC TTTTAATCCT TCAGAAGATG ACTATGAAGA GGAAGAGGAG 
GACAGTGATG AAGATTATTC ATCAGAAGCA GAAGAGTCAG ACTATTCTAA GGAGTCATTG 
GGTAGTGAAG AAGAGAGTGG AAAGGATTGG GATGAACTGG AGGAAGAAGC CCGAAAAGCG 
GACCGAGAAA GTCGTTACGA GGAAGAAGAA GAACAAAGTC GAAGTATGAG CCGGAAGAGG 
AAGGCATCTG TGCACAGTTC GGGCCGTGGC TCTAACCGTG GTTCCAGACA CAGCTCTGCA 
CCCCCCAAGA AAAAGAGGAA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SUPT16H ( NM_007192.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_009123.1 Homo sapiens SPT16 homolog, facilitates chromatin remodeling subunit (SUPT16H), mRNA.

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CloneID	OHu22815
Clone ID Related Accession (Same CDS sequence)	NM_007192.3 , NM_007192.4
Accession Version	NM_007192.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3144bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-03
Organism	Homo sapiens(human)
Product	FACT complex subunit SPT16
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK024072.1, AK001719.1, BC014046.2, BP872403.1 and AL135744.4. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_007192.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.184381.1, SRR1803612.96622.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000216297.7/ ENSP00000216297.2 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCTGTGA CTCTGGACAA AGACGCTTAT TATCGGCGAG TGAAGAGACT GTACAGCAAT 
TGGCGGAAAG GAGAAGATGA GTATGCCAAC GTTGATGCCA TTGTTGTATC AGTGGGTGTT 
GATGAAGAAA TTGTTTATGC CAAATCAACT GCCTTACAGA CATGGCTCTT TGGTTATGAA 
CTAACTGATA CTATCATGGT CTTTTGTGAT GACAAAATCA TCTTTATGGC CAGCAAGAAA 
AAAGTGGAGT TCTTGAAACA GATTGCCAAC ACTAAGGGCA ATGAGAATGC TAATGGAGCC 
CCTGCCATCA CACTGCTAAT ACGAGAAAAG AATGAAAGTA ATAAGAGTAG CTTTGACAAA 
ATGATTGAAG CCATTAAAGA AAGCAAGAAT GGCAAGAAGA TTGGAGTGTT CAGCAAAGAC 
AAATTCCCTG GAGAGTTCAT GAAGAGCTGG AATGACTGCC TCAACAAAGA AGGCTTTGAC 
AAAATAGATA TCAGTGCAGT TGTGGCATAT ACCATCGCTG TAAAGGAGGA TGGGGAGCTC 
AACCTAATGA AGAAAGCAGC CAGCATCACT TCTGAAGTCT TCAACAAATT CTTCAAGGAA 
AGAGTCATGG AAATAGTTGA TGCAGATGAG AAAGTTCGAC ACAGCAAACT GGCTGAGTCT 
GTGGAAAAGG CCATTGAAGA GAAAAAATAC CTTGCTGGGG CAGACCCTTC TACTGTGGAA 
ATGTGTTACC CTCCTATCAT TCAGAGTGGT GGCAACTATA ATCTCAAGTT CAGTGTGGTG 
AGTGACAAGA ATCATATGCA CTTTGGGGCT ATCACTTGTG CCATGGGTAT TCGCTTCAAG 
TCTTACTGCT CCAACCTTGT TCGCACTTTG ATGGTTGATC CTTCTCAAGA AGTTCAAGAA 
AATTATAACT TTTTGCTCCA GCTTCAAGAG GAGCTGCTGA AGGAATTAAG ACATGGTGTG 
AAGATATGTG ACGTGTATAA CGCTGTCATG GACGTGGTTA AAAAGCAGAA GCCAGAACTG 
CTGAACAAAA TTACCAAAAA CCTAGGGTTT GGGATGGGAA TTGAATTCCG TGAAGGCTCC 
CTAGTAATCA ATAGCAAAAA TCAATACAAA CTGAAGAAAG GAATGGTTTT CAGCATCAAT 
TTAGGATTCT CAGACCTGAC TAACAAGGAG GGGAAAAAGC CAGAAGAGAA AACCTATGCC 
CTGTTCATTG GTGACACAGT GCTTGTGGAT GAGGATGGCC CAGCTACTGT TCTCACTTCT 
GTGAAGAAGA AAGTGAAGAA TGTGGGGATT TTCCTAAAGA ATGAAGATGA GGAAGAAGAG 
GAGGAGGAGA AAGATGAGGC AGAGGACCTT TTGGGAAGAG GTTCTCGGGC AGCATTACTT 
ACAGAAAGAA CAAGAAATGA AATGACTGCA GAAGAGAAGC GAAGAGCACA TCAGAAAGAA 
CTAGCGGCTC AACTCAATGA AGAAGCAAAG AGGCGATTGA CTGAACAAAA GGGAGAACAG 
CAGATTCAGA AAGCTCGCAA GTCTAATGTG TCCTATAAAA ACCCATCTCT GATGCCTAAG 
GAACCACATA TTCGGGAAAT GAAGATCTAC ATCGATAAGA AATATGAGAC TGTAATAATG 
CCCGTGTTTG GCATTGCAAC ACCGTTTCAC ATTGCCACAA TCAAGAATAT AAGTATGTCC 
GTGGAAGGAG ATTATACTTA CTTGCGAATC AACTTTTATT GCCCAGGCAG TGCTCTGGGC 
AGGAATGAAG GCAACATCTT TCCTAACCCT GAAGCGACTT TTGTCAAGGA AATTACATAC 
CGAGCATCAA ATATTAAGGC ACCCGGAGAA CAGACAGTAC CAGCCTTGAA CCTTCAGAAT 
GCTTTCCGAA TTATTAAAGA AGTACAGAAA CGTTATAAAA CTCGAGAAGC TGAAGAGAAA 
GAGAAGGAGG GGATTGTAAA ACAAGACTCA CTGGTGATCA ATCTAAACCG GAGTAATCCG 
AAACTGAAAG ATCTATACAT TCGCCCAAAT ATTGCCCAAA AGAGGATGCA AGGCTCACTG 
GAGGCCCATG TCAATGGCTT CCGCTTCACA TCTGTTCGAG GAGACAAAGT GGATATTTTG 
TACAATAATA TTAAGCATGC TTTGTTCCAG CCCTGTGATG GAGAAATGAT TATTGTCTTG 
CACTTTCACC TCAAGAATGC CATCATGTTT GGGAAGAAGC GGCACACGGA TGTGCAGTTC 
TACACAGAAG TGGGAGAGAT AACCACGGAC TTGGGGAAAC ATCAGCATAT GCATGACCGA 
GATGACCTCT ATGCTGAGCA GATGGAACGA GAAATGAGGC ACAAACTGAA AACAGCCTTT 
AAAAATTTCA TTGAGAAAGT AGAGGCTCTA ACTAAGGAGG AACTGGAATT TGAAGTGCCT 
TTTAGGGACT TGGGATTTAA CGGAGCTCCC TATAGGAGTA CCTGCCTCCT TCAGCCCACT 
AGTAGTGCGC TGGTAAATGC TACGGAATGG CCACCTTTTG TGGTGACATT GGATGAGGTA 
GAGCTGATCC ACTTTGAGCG GGTCCAGTTT CACCTGAAGA ACTTTGATAT GGTAATCGTC 
TACAAGGACT ACAGCAAGAA AGTGACCATG ATCAACGCCA TTCCTGTAGC CTCTCTTGAC 
CCCATCAAGG AATGGTTGAA TTCCTGCGAC CTGAAATACA CAGAAGGAGT ACAGTCCCTC 
AACTGGACTA AAATCATGAA GACCATTGTT GATGACCCTG AGGGCTTCTT CGAACAAGGT 
GGCTGGTCTT TCCTGGAGCC TGAGGGTGAG GGGAGTGATG CTGAAGAAGG GGATTCAGAG 
TCTGAAATTG AAGATGAGAC TTTTAATCCT TCAGAAGATG ACTATGAAGA GGAAGAGGAG 
GACAGTGATG AAGATTATTC ATCAGAAGCA GAAGAGTCAG ACTATTCTAA GGAGTCATTG 
GGTAGTGAAG AAGAGAGTGG AAAGGATTGG GATGAACTGG AGGAAGAAGC CCGAAAAGCG 
GACCGAGAAA GTCGTTACGA GGAAGAAGAA GAACAAAGTC GAAGTATGAG CCGGAAGAGG 
AAGGCATCTG TGCACAGTTC GGGCCGTGGC TCTAACCGTG GTTCCAGACA CAGCTCTGCA 
CCCCCCAAGA AAAAGAGGAA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_009123.1
CDS	93..3236
Translation	MAVTLDKDAYYRRVKRLYSNWRKGEDEYANVDAIVVSVGVDEEIVYAKSTALQTWLFGYELTDTIMVFCDDKIIFMASKKKVEFLKQIANTKGNENANGAPAITLLIREKNESNKSSFDKMIEAIKESKNGKKIGVFSKDKFPGEFMKSWNDCLNKEGFDKIDISAVVAYTIAVKEDGELNLMKKAASITSEVFNKFFKERVMEIVDADEKVRHSKLAESVEKAIEEKKYLAGADPSTVEMCYPPIIQSGGNYNLKFSVVSDKNHMHFGAITCAMGIRFKSYCSNLVRTLMVDPSQEVQENYNFLLQLQEELLKELRHGVKICDVYNAVMDVVKKQKPELLNKITKNLGFGMGIEFREGSLVINSKNQYKLKKGMVFSINLGFSDLTNKEGKKPEEKTYALFIGDTVLVDEDGPATVLTSVKKKVKNVGIFLKNEDEEEEEEEKDEAEDLLGRGSRAALLTERTRNEMTAEEKRRAHQKELAAQLNEEAKRRLTEQKGEQQIQKARKSNVSYKNPSLMPKEPHIREMKIYIDKKYETVIMPVFGIATPFHIATIKNISMSVEGDYTYLRINFYCPGSALGRNEGNIFPNPEATFVKEITYRASNIKAPGEQTVPALNLQNAFRIIKEVQKRYKTREAEEKEKEGIVKQDSLVINLNRSNPKLKDLYIRPNIAQKRMQGSLEAHVNGFRFTSVRGDKVDILYNNIKHALFQPCDGEMIIVLHFHLKNAIMFGKKRHTDVQFYTEVGEITTDLGKHQHMHDRDDLYAEQMEREMRHKLKTAFKNFIEKVEALTKEELEFEVPFRDLGFNGAPYRSTCLLQPTSSALVNATEWPPFVVTLDEVELIHFERVQFHLKNFDMVIVYKDYSKKVTMINAIPVASLDPIKEWLNSCDLKYTEGVQSLNWTKIMKTIVDDPEGFFEQGGWSFLEPEGEGSDAEEGDSESEIEDETFNPSEDDYEEEEEDSDEDYSSEAEESDYSKESLGSEEESGKDWDELEEEARKADRESRYEEEEEQSRSMSRKRKASVHSSGRGSNRGSRHSSAPPKKKRK

Target ORF information:

RefSeq Version	NM_007192.4
Organism	Homo sapiens(human)
Definition	Homo sapiens SPT16 homolog, facilitates chromatin remodeling subunit (SUPT16H), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007192.4

ORF Insert Sequence:

ATGGCTGTGA CTCTGGACAA AGACGCTTAT TATCGGCGAG TGAAGAGACT GTACAGCAAT 
TGGCGGAAAG GAGAAGATGA GTATGCCAAC GTTGATGCCA TTGTTGTATC AGTGGGTGTT 
GATGAAGAAA TTGTTTATGC CAAATCAACT GCCTTACAGA CATGGCTCTT TGGTTATGAA 
CTAACTGATA CTATCATGGT CTTTTGTGAT GACAAAATCA TCTTTATGGC CAGCAAGAAA 
AAAGTGGAGT TCTTGAAACA GATTGCCAAC ACTAAGGGCA ATGAGAATGC TAATGGAGCC 
CCTGCCATCA CACTGCTAAT ACGAGAAAAG AATGAAAGTA ATAAGAGTAG CTTTGACAAA 
ATGATTGAAG CCATTAAAGA AAGCAAGAAT GGCAAGAAGA TTGGAGTGTT CAGCAAAGAC 
AAATTCCCTG GAGAGTTCAT GAAGAGCTGG AATGACTGCC TCAACAAAGA AGGCTTTGAC 
AAAATAGATA TCAGTGCAGT TGTGGCATAT ACCATCGCTG TAAAGGAGGA TGGGGAGCTC 
AACCTAATGA AGAAAGCAGC CAGCATCACT TCTGAAGTCT TCAACAAATT CTTCAAGGAA 
AGAGTCATGG AAATAGTTGA TGCAGATGAG AAAGTTCGAC ACAGCAAACT GGCTGAGTCT 
GTGGAAAAGG CCATTGAAGA GAAAAAATAC CTTGCTGGGG CAGACCCTTC TACTGTGGAA 
ATGTGTTACC CTCCTATCAT TCAGAGTGGT GGCAACTATA ATCTCAAGTT CAGTGTGGTG 
AGTGACAAGA ATCATATGCA CTTTGGGGCT ATCACTTGTG CCATGGGTAT TCGCTTCAAG 
TCTTACTGCT CCAACCTTGT TCGCACTTTG ATGGTTGATC CTTCTCAAGA AGTTCAAGAA 
AATTATAACT TTTTGCTCCA GCTTCAAGAG GAGCTGCTGA AGGAATTAAG ACATGGTGTG 
AAGATATGTG ACGTGTATAA CGCTGTCATG GACGTGGTTA AAAAGCAGAA GCCAGAACTG 
CTGAACAAAA TTACCAAAAA CCTAGGGTTT GGGATGGGAA TTGAATTCCG TGAAGGCTCC 
CTAGTAATCA ATAGCAAAAA TCAATACAAA CTGAAGAAAG GAATGGTTTT CAGCATCAAT 
TTAGGATTCT CAGACCTGAC TAACAAGGAG GGGAAAAAGC CAGAAGAGAA AACCTATGCC 
CTGTTCATTG GTGACACAGT GCTTGTGGAT GAGGATGGCC CAGCTACTGT TCTCACTTCT 
GTGAAGAAGA AAGTGAAGAA TGTGGGGATT TTCCTAAAGA ATGAAGATGA GGAAGAAGAG 
GAGGAGGAGA AAGATGAGGC AGAGGACCTT TTGGGAAGAG GTTCTCGGGC AGCATTACTT 
ACAGAAAGAA CAAGAAATGA AATGACTGCA GAAGAGAAGC GAAGAGCACA TCAGAAAGAA 
CTAGCGGCTC AACTCAATGA AGAAGCAAAG AGGCGATTGA CTGAACAAAA GGGAGAACAG 
CAGATTCAGA AAGCTCGCAA GTCTAATGTG TCCTATAAAA ACCCATCTCT GATGCCTAAG 
GAACCACATA TTCGGGAAAT GAAGATCTAC ATCGATAAGA AATATGAGAC TGTAATAATG 
CCCGTGTTTG GCATTGCAAC ACCGTTTCAC ATTGCCACAA TCAAGAATAT AAGTATGTCC 
GTGGAAGGAG ATTATACTTA CTTGCGAATC AACTTTTATT GCCCAGGCAG TGCTCTGGGC 
AGGAATGAAG GCAACATCTT TCCTAACCCT GAAGCGACTT TTGTCAAGGA AATTACATAC 
CGAGCATCAA ATATTAAGGC ACCCGGAGAA CAGACAGTAC CAGCCTTGAA CCTTCAGAAT 
GCTTTCCGAA TTATTAAAGA AGTACAGAAA CGTTATAAAA CTCGAGAAGC TGAAGAGAAA 
GAGAAGGAGG GGATTGTAAA ACAAGACTCA CTGGTGATCA ATCTAAACCG GAGTAATCCG 
AAACTGAAAG ATCTATACAT TCGCCCAAAT ATTGCCCAAA AGAGGATGCA AGGCTCACTG 
GAGGCCCATG TCAATGGCTT CCGCTTCACA TCTGTTCGAG GAGACAAAGT GGATATTTTG 
TACAATAATA TTAAGCATGC TTTGTTCCAG CCCTGTGATG GAGAAATGAT TATTGTCTTG 
CACTTTCACC TCAAGAATGC CATCATGTTT GGGAAGAAGC GGCACACGGA TGTGCAGTTC 
TACACAGAAG TGGGAGAGAT AACCACGGAC TTGGGGAAAC ATCAGCATAT GCATGACCGA 
GATGACCTCT ATGCTGAGCA GATGGAACGA GAAATGAGGC ACAAACTGAA AACAGCCTTT 
AAAAATTTCA TTGAGAAAGT AGAGGCTCTA ACTAAGGAGG AACTGGAATT TGAAGTGCCT 
TTTAGGGACT TGGGATTTAA CGGAGCTCCC TATAGGAGTA CCTGCCTCCT TCAGCCCACT 
AGTAGTGCGC TGGTAAATGC TACGGAATGG CCACCTTTTG TGGTGACATT GGATGAGGTA 
GAGCTGATCC ACTTTGAGCG GGTCCAGTTT CACCTGAAGA ACTTTGATAT GGTAATCGTC 
TACAAGGACT ACAGCAAGAA AGTGACCATG ATCAACGCCA TTCCTGTAGC CTCTCTTGAC 
CCCATCAAGG AATGGTTGAA TTCCTGCGAC CTGAAATACA CAGAAGGAGT ACAGTCCCTC 
AACTGGACTA AAATCATGAA GACCATTGTT GATGACCCTG AGGGCTTCTT CGAACAAGGT 
GGCTGGTCTT TCCTGGAGCC TGAGGGTGAG GGGAGTGATG CTGAAGAAGG GGATTCAGAG 
TCTGAAATTG AAGATGAGAC TTTTAATCCT TCAGAAGATG ACTATGAAGA GGAAGAGGAG 
GACAGTGATG AAGATTATTC ATCAGAAGCA GAAGAGTCAG ACTATTCTAA GGAGTCATTG 
GGTAGTGAAG AAGAGAGTGG AAAGGATTGG GATGAACTGG AGGAAGAAGC CCGAAAAGCG 
GACCGAGAAA GTCGTTACGA GGAAGAAGAA GAACAAAGTC GAAGTATGAG CCGGAAGAGG 
AAGGCATCTG TGCACAGTTC GGGCCGTGGC TCTAACCGTG GTTCCAGACA CAGCTCTGCA 
CCCCCCAAGA AAAAGAGGAA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

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SUPT16H cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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