MAP4K1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	MAP4K1
Entrez Gene ID	11184
Full Name	mitogen-activated protein kinase kinase kinase kinase 1
Synonyms	HPK1
General protein information	Preferred Names mitogen-activated protein kinase kinase kinase kinase 1 Names mitogen-activated protein kinase kinase kinase kinase 1 MAPK/ERK kinase kinase kinase 1 MEK kinase kinase 1 MEKKK 1 hematopoietic progenitor kinase 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 19 Map Location: 19q13.2
Disorder MIM:	601983

mRNA and Protein(s)

mRNA	Protein	Name
NM_001042600.2	NP_001036065.1	mitogen-activated protein kinase kinase kinase kinase 1 isoform 1
NM_007181.5	NP_009112.1	mitogen-activated protein kinase kinase kinase kinase 1 isoform 2
NM_001042600.3	NP_001036065.1	mitogen-activated protein kinase kinase kinase kinase 1 isoform 1
XM_011526403.1	XP_011524705.1	mitogen-activated protein kinase kinase kinase kinase 1 isoform X1
NM_007181.6	NP_009112.1	mitogen-activated protein kinase kinase kinase kinase 1 isoform 2
XM_011526404.1	XP_011524706.1	mitogen-activated protein kinase kinase kinase kinase 1 isoform X2
XM_017026231.1	XP_016881720.1	mitogen-activated protein kinase kinase kinase kinase 1 isoform X3

Pathways from BioSystems

Homologies

Bos taurus (cattle)	MAP4K1	NP_001069293.2
Rattus norvegicus (Norway rat)	Map4k1	NP_001099713.1
Canis lupus familiaris (dog)	MAP4K1	XP_541641.3
Mus musculus (house mouse)	Map4k1	NP_032305.2
Homo sapiens (human)	MAP4K1	NP_001036065.1
Pan troglodytes (chimpanzee)	MAP4K1	XP_003316364.1
	MAP4K1	XP_001082963.2
Xenopus tropicalis (tropical clawed frog)	map4k1	XP_002939295.2

Gene Ontology
Bibliography

Related articles in PubMed

Interactions between hematopoietic progenitor kinase 1 and its adaptor proteins (Review).
Zhang Q, Ding S, Zhang H
Molecular medicine reports16(5)6472-6482(2017 Nov)

HPK1 positive expression associated with longer overall survival in patients with estrogen receptor-positive invasive ductal carcinoma?not otherwise specified.
Wang J, Song L, Yang S, Zhang W, Lu P, Li S, Li H, Wang L
Molecular medicine reports16(4)4634-4642(2017 Oct)

The CUL7/F-box and WD repeat domain containing 8 (CUL7/Fbxw8) ubiquitin ligase promotes degradation of hematopoietic progenitor kinase 1.
Wang H, Chen Y, Lin P, Li L, Zhou G, Liu G, Logsdon C, Jin J, Abbruzzese JL, Tan TH, Wang H
The Journal of biological chemistry289(7)4009-17(2014 Feb)

Hematopoietic progenitor kinase 1 (HPK1) is required for LFA-1-mediated neutrophil recruitment during the acute inflammatory response.
Jakob SM, Pick R, Brechtefeld D, Nussbaum C, Kiefer F, Sperandio M, Walzog B
Blood121(20)4184-94(2013 May)

Pdcd4 knockdown up-regulates MAP4K1 expression and activation of AP-1 dependent transcription through c-Myc.
Wang Q, Zhang Y, Yang HS
Biochimica et biophysica acta1823(10)1807-14(2012 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

CUL7/Fbxw8 ubiquitin ligase-mediated HPK1 degradation revealed a direct link and novel role of CUL7/Fbxw8 ubiquitin ligase in the MAPK pathway, which plays a critical role in cell proliferation and differentiation.
Title: The CUL7/F-box and WD repeat domain containing 8 (CUL7/Fbxw8) ubiquitin ligase promotes degradation of hematopoietic progenitor kinase 1.

HPK1 is critically involved in LFA-1-mediated polymorphonuclear neutrophils trafficking during acute inflammation.
Title: Hematopoietic progenitor kinase 1 (HPK1) is required for LFA-1-mediated neutrophil recruitment during the acute inflammatory response.

Pdcd4 knockdown up-regulates MAP kinase kinase kinase kinase 1 (MAP4K1) expression and increases phosphorylation of c-Jun.
Title: Pdcd4 knockdown up-regulates MAP4K1 expression and activation of AP-1 dependent transcription through c-Myc.

QVD and 1,25D-induced differentiation was accompanied by increased signaling by Hematopoietic Progenitor Kinase 1(HPK1), and the expression of transcription factors known to be involved in monocytic differentiation was increased.
Title: The pan-caspase inhibitor Q-VD-OPh has anti-leukemia effects and can interact with vitamin D analogs to increase HPK1 signaling in AML cells.

results indicate that uncleaved HPK1 is a positive regulator of vitamin D-induced differentiation in acute myeloid leukemia cells, but the cleaved HPK1 fragment inhibits differentiation
Title: Dual role of hematopoietic progenitor kinase 1 (HPK1) as a positive regulator of 1?,25-dihydroxyvitamin D-induced differentiation and cell cycle arrest of AML cells and as a mediator of vitamin D resistance.

The following MAP4K1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MAP4K1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu20964	NM_001042600.3 Latest version!	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 1 (MAP4K1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$414.50-$580.30 $829.00
OHu21316	NM_007181.6 Latest version!	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 1 (MAP4K1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$419.50-$587.30 $839.00
OHu64825	XM_011526403.1 Latest version!	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 1 (MAP4K1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu64826	XM_011526404.1 Latest version!	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 1 (MAP4K1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu88172	XM_017026231.1 Latest version!	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 1 (MAP4K1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
View Old Accession Versions >>
OHu20964	NM_001042600.2	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 1 (MAP4K1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$414.50-$580.30 $829.00
OHu21316	NM_007181.5	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 1 (MAP4K1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$419.50-$587.30 $839.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MAP4K1 ( NM_001042600.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001036065.1 Homo sapiens mitogen-activated protein kinase kinase kinase kinase 1 (MAP4K1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu20964
Clone ID Related Accession (Same CDS sequence)	NM_001042600.2 , NM_001042600.3
Accession Version	NM_001042600.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2466bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-02
Organism	Homo sapiens(human)
Product	mitogen-activated protein kinase kinase kinase kinase 1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from HY074876.1, U66464.1 and AA995240.1. On Nov 16, 2013 this sequence version replaced NM_001042600.1. Transcript Variant: This variant (1) lacks an exon in the 3' coding region, which results in a frameshift, compared to variant 2. The encoded isoform (1) has a shorter and distinct C-terminus, compared to isoform 2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGACGTCG TGGACCCTGA CATTTTCAAT AGAGACCCCC GGGACCACTA TGACCTGCTA 
CAGCGGCTGG GTGGCGGCAC GTATGGGGAA GTCTTTAAGG CTCGAGACAA GGTGTCAGGG 
GACCTGGTGG CACTGAAGAT GGTGAAGATG GAGCCTGATG ATGATGTCTC CACCCTTCAG 
AAGGAAATCC TCATATTGAA AACTTGCCGG CACGCCAACA TCGTGGCCTA CCATGGGAGT 
TATCTCTGGT TGCAGAAACT CTGGATCTGC ATGGAATTCT GTGGGGCTGG TTCTCTCCAG 
GACATCTACC AAGTGACAGG CTCCCTGTCA GAGCTCCAGA TTAGCTATGT CTGCCGGGAA 
GTGCTCCAGG GACTGGCCTA TTTGCACTCA CAGAAGAAGA TACACAGGGA CATCAAGGGA 
GCTAACATCC TCATCAATGA TGCTGGGGAG GTCAGATTGG CTGACTTTGG CATCTCGGCC 
CAGATTGGGG CTACACTGGC CAGACGCCTC TCTTTCATTG GGACACCCTA CTGGATGGCT 
CCGGAAGTGG CAGCTGTGGC CCTGAAGGGA GGATACAATG AGCTGTGTGA CATCTGGTCC 
CTGGGCATCA CGGCCATCGA ACTGGCCGAG CTACAGCCAC CGCTCTTTGA TGTGCACCCT 
CTCAGAGTTC TCTTCCTCAT GACCAAGAGT GGCTACCAGC CTCCCCGACT GAAGGAAAAA 
GGCAAATGGT CGGCTGCCTT CCACAACTTC ATCAAAGTCA CTCTGACTAA GAGTCCCAAG 
AAACGACCCA GCGCCACCAA GATGCTCAGT CATCAACTGG TATCCCAGCC TGGGCTGAAT 
CGAGGCCTGA TCCTGGATCT TCTTGACAAA CTGAAGAATC CCGGGAAAGG ACCCTCCATT 
GGGGACATTG AGGATGAGGA GCCCGAGCTA CCCCCTGCTA TCCCTCGGCG GATCAGATCC 
ACCCACCGCT CCAGCTCTCT GGGGATCCCA GATGCAGACT GCTGTCGGCG GCACATGGAG 
TTCAGGAAGC TCCGAGGAAT GGAGACCAGA CCCCCAGCCA ACACCGCTCG CCTACAGCCT 
CCTCGAGACC TCAGGAGCAG CAGCCCCAGG AAGCAACTGT CAGAGTCGTC TGACGATGAC 
TATGACGACG TGGACATCCC CACCCCTGCA GAGGACACAC CTCCTCCACT TCCCCCCAAG 
CCCAAGTTCC GTTCTCCATC AGACGAGGGT CCTGGGAGCA TGGGGGATGA TGGGCAGCTG 
AGCCCGGGGG TGCTGGTCCG GTGTGCCAGT GGGCCCCCAC CAAACAGCCC CCGTCCTGGG 
CCTCCCCCAT CCACCAGCAG CCCCCACCTC ACCGCCCATT CAGAACCCTC ACTCTGGAAC 
CCACCCTCCC GGGAGCTTGA CAAGCCCCCA CTTCTGCCCC CCAAGAAGGA AAAGATGAAG 
AGAAAGGGAT GTGCCCTTCT CGTAAAGTTG TTCAATGGCT GCCCCCTCCG GATCCACAGC 
ACGGCCGCCT GGACACATCC CTCCACCAAG GACCAGCACC TGCTCCTGGG GGCAGAGGAA 
GGCATCTTCA TCCTGAACCG GAATGACCAG GAGGCCACGC TGGAAATGCT CTTTCCTAGC 
CGGACTACGT GGGTGTACTC CATCAACAAC GTTCTCATGT CTCTCTCAGG AAAGACCCCC 
CACCTGTATT CTCATAGCAT CCTTGGCCTG CTGGAACGGA AAGAGACCAG AGCAGGAAAC 
CCCATCGCTC ACATTAGCCC CCACCGCCTA CTGGCAAGGA AGAACATGGT TTCCACCAAG 
ATCCAGGACA CCAAAGGCTG CCGGGCGTGC TGTGTGGCGG AGGGTGCGAG CTCTGGGGGC 
CCGTTCCTGT GCGGTGCATT GGAGACGTCC GTTGTCCTGC TTCAGTGGTA CCAGCCCATG 
AACAAATTCC TGCTTGTCCG GCAGGTGCTG TTCCCACTGC CGACGCCTCT GTCCGTGTTC 
GCGCTGCTGA CCGGGCCAGG CTCTGAGCTG CCCGCTGTGT GCATCGGCGT GAGCCCCGGG 
CGGCCGGGGA AGTCGGTGCT CTTCCACACG GTGCGCTTTG GCGCGCTCTC TTGCTGGCTG 
GGCGAGATGA GCACCGAGCA CAGGGGACCC GTGCAGGTGA CCCAGGTAGA GGAAGATATG 
GTGATGGTGT TGATGGATGG CTCTGTGAAG CTGGTGACCC CGGAGGGGTC CCCAGTCCGG 
GGACTTCGCA CACCTGAGAT CCCCATGACC GAAGCGGTGG AGGCCGTGGC TATGGTTGGA 
GGTCAGCTTC AGGCCTTCTG GAAGCATGGA GTGCAGGTGT GGGCTCTAGG CTCGGATCAG 
CTGCTACAGG AGCTGAGAGA CCCTACCCTC ACTTTCCGTC TGCTTGGCTC CCCCAGGCCT 
GTAGTGGTGG AGACACGCCC AGTGGATGAT CCTACTGCTC CCAGCAACCT CTACATCCAG 
GAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001036065.1
CDS	141..2606
Translation	MDVVDPDIFNRDPRDHYDLLQRLGGGTYGEVFKARDKVSGDLVALKMVKMEPDDDVSTLQKEILILKTCRHANIVAYHGSYLWLQKLWICMEFCGAGSLQDIYQVTGSLSELQISYVCREVLQGLAYLHSQKKIHRDIKGANILINDAGEVRLADFGISAQIGATLARRLSFIGTPYWMAPEVAAVALKGGYNELCDIWSLGITAIELAELQPPLFDVHPLRVLFLMTKSGYQPPRLKEKGKWSAAFHNFIKVTLTKSPKKRPSATKMLSHQLVSQPGLNRGLILDLLDKLKNPGKGPSIGDIEDEEPELPPAIPRRIRSTHRSSSLGIPDADCCRRHMEFRKLRGMETRPPANTARLQPPRDLRSSSPRKQLSESSDDDYDDVDIPTPAEDTPPPLPPKPKFRSPSDEGPGSMGDDGQLSPGVLVRCASGPPPNSPRPGPPPSTSSPHLTAHSEPSLWNPPSRELDKPPLLPPKKEKMKRKGCALLVKLFNGCPLRIHSTAAWTHPSTKDQHLLLGAEEGIFILNRNDQEATLEMLFPSRTTWVYSINNVLMSLSGKTPHLYSHSILGLLERKETRAGNPIAHISPHRLLARKNMVSTKIQDTKGCRACCVAEGASSGGPFLCGALETSVVLLQWYQPMNKFLLVRQVLFPLPTPLSVFALLTGPGSELPAVCIGVSPGRPGKSVLFHTVRFGALSCWLGEMSTEHRGPVQVTQVEEDMVMVLMDGSVKLVTPEGSPVRGLRTPEIPMTEAVEAVAMVGGQLQAFWKHGVQVWALGSDQLLQELRDPTLTFRLLGSPRPVVVETRPVDDPTAPSNLYIQE

Target ORF information:

RefSeq Version	NM_001042600.2
Organism	Homo sapiens(human)
Definition	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 1 (MAP4K1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001042600.2

ORF Insert Sequence:

ATGGACGTCG TGGACCCTGA CATTTTCAAT AGAGACCCCC GGGACCACTA TGACCTGCTA 
CAGCGGCTGG GTGGCGGCAC GTATGGGGAA GTCTTTAAGG CTCGAGACAA GGTGTCAGGG 
GACCTGGTGG CACTGAAGAT GGTGAAGATG GAGCCTGATG ATGATGTCTC CACCCTTCAG 
AAGGAAATCC TCATATTGAA AACTTGCCGG CACGCCAACA TCGTGGCCTA CCATGGGAGT 
TATCTCTGGT TGCAGAAACT CTGGATCTGC ATGGAATTCT GTGGGGCTGG TTCTCTCCAG 
GACATCTACC AAGTGACAGG CTCCCTGTCA GAGCTCCAGA TTAGCTATGT CTGCCGGGAA 
GTGCTCCAGG GACTGGCCTA TTTGCACTCA CAGAAGAAGA TACACAGGGA CATCAAGGGA 
GCTAACATCC TCATCAATGA TGCTGGGGAG GTCAGATTGG CTGACTTTGG CATCTCGGCC 
CAGATTGGGG CTACACTGGC CAGACGCCTC TCTTTCATTG GGACACCCTA CTGGATGGCT 
CCGGAAGTGG CAGCTGTGGC CCTGAAGGGA GGATACAATG AGCTGTGTGA CATCTGGTCC 
CTGGGCATCA CGGCCATCGA ACTGGCCGAG CTACAGCCAC CGCTCTTTGA TGTGCACCCT 
CTCAGAGTTC TCTTCCTCAT GACCAAGAGT GGCTACCAGC CTCCCCGACT GAAGGAAAAA 
GGCAAATGGT CGGCTGCCTT CCACAACTTC ATCAAAGTCA CTCTGACTAA GAGTCCCAAG 
AAACGACCCA GCGCCACCAA GATGCTCAGT CATCAACTGG TATCCCAGCC TGGGCTGAAT 
CGAGGCCTGA TCCTGGATCT TCTTGACAAA CTGAAGAATC CCGGGAAAGG ACCCTCCATT 
GGGGACATTG AGGATGAGGA GCCCGAGCTA CCCCCTGCTA TCCCTCGGCG GATCAGATCC 
ACCCACCGCT CCAGCTCTCT GGGGATCCCA GATGCAGACT GCTGTCGGCG GCACATGGAG 
TTCAGGAAGC TCCGAGGAAT GGAGACCAGA CCCCCAGCCA ACACCGCTCG CCTACAGCCT 
CCTCGAGACC TCAGGAGCAG CAGCCCCAGG AAGCAACTGT CAGAGTCGTC TGACGATGAC 
TATGACGACG TGGACATCCC CACCCCTGCA GAGGACACAC CTCCTCCACT TCCCCCCAAG 
CCCAAGTTCC GTTCTCCATC AGACGAGGGT CCTGGGAGCA TGGGGGATGA TGGGCAGCTG 
AGCCCGGGGG TGCTGGTCCG GTGTGCCAGT GGGCCCCCAC CAAACAGCCC CCGTCCTGGG 
CCTCCCCCAT CCACCAGCAG CCCCCACCTC ACCGCCCATT CAGAACCCTC ACTCTGGAAC 
CCACCCTCCC GGGAGCTTGA CAAGCCCCCA CTTCTGCCCC CCAAGAAGGA AAAGATGAAG 
AGAAAGGGAT GTGCCCTTCT CGTAAAGTTG TTCAATGGCT GCCCCCTCCG GATCCACAGC 
ACGGCCGCCT GGACACATCC CTCCACCAAG GACCAGCACC TGCTCCTGGG GGCAGAGGAA 
GGCATCTTCA TCCTGAACCG GAATGACCAG GAGGCCACGC TGGAAATGCT CTTTCCTAGC 
CGGACTACGT GGGTGTACTC CATCAACAAC GTTCTCATGT CTCTCTCAGG AAAGACCCCC 
CACCTGTATT CTCATAGCAT CCTTGGCCTG CTGGAACGGA AAGAGACCAG AGCAGGAAAC 
CCCATCGCTC ACATTAGCCC CCACCGCCTA CTGGCAAGGA AGAACATGGT TTCCACCAAG 
ATCCAGGACA CCAAAGGCTG CCGGGCGTGC TGTGTGGCGG AGGGTGCGAG CTCTGGGGGC 
CCGTTCCTGT GCGGTGCATT GGAGACGTCC GTTGTCCTGC TTCAGTGGTA CCAGCCCATG 
AACAAATTCC TGCTTGTCCG GCAGGTGCTG TTCCCACTGC CGACGCCTCT GTCCGTGTTC 
GCGCTGCTGA CCGGGCCAGG CTCTGAGCTG CCCGCTGTGT GCATCGGCGT GAGCCCCGGG 
CGGCCGGGGA AGTCGGTGCT CTTCCACACG GTGCGCTTTG GCGCGCTCTC TTGCTGGCTG 
GGCGAGATGA GCACCGAGCA CAGGGGACCC GTGCAGGTGA CCCAGGTAGA GGAAGATATG 
GTGATGGTGT TGATGGATGG CTCTGTGAAG CTGGTGACCC CGGAGGGGTC CCCAGTCCGG 
GGACTTCGCA CACCTGAGAT CCCCATGACC GAAGCGGTGG AGGCCGTGGC TATGGTTGGA 
GGTCAGCTTC AGGCCTTCTG GAAGCATGGA GTGCAGGTGT GGGCTCTAGG CTCGGATCAG 
CTGCTACAGG AGCTGAGAGA CCCTACCCTC ACTTTCCGTC TGCTTGGCTC CCCCAGGCCT 
GTAGTGGTGG AGACACGCCC AGTGGATGAT CCTACTGCTC CCAGCAACCT CTACATCCAG 
GAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP4K1 ( NM_001042600.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001036065.1 Homo sapiens mitogen-activated protein kinase kinase kinase kinase 1 (MAP4K1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu20964
Clone ID Related Accession (Same CDS sequence)	NM_001042600.2 , NM_001042600.3
Accession Version	NM_001042600.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2466bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-07
Organism	Homo sapiens(human)
Product	mitogen-activated protein kinase kinase kinase kinase 1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from HY074876.1 and U66464.1. On Nov 22, 2018 this sequence version replaced NM_001042600.2. Transcript Variant: This variant (1) lacks an exon in the 3' coding region, which results in a frameshift, compared to variant 2. The encoded isoform (1) has a shorter and distinct C-terminus, compared to isoform 2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163655.43428.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000396857.7/ ENSP00000380066.1 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGACGTCG TGGACCCTGA CATTTTCAAT AGAGACCCCC GGGACCACTA TGACCTGCTA 
CAGCGGCTGG GTGGCGGCAC GTATGGGGAA GTCTTTAAGG CTCGAGACAA GGTGTCAGGG 
GACCTGGTGG CACTGAAGAT GGTGAAGATG GAGCCTGATG ATGATGTCTC CACCCTTCAG 
AAGGAAATCC TCATATTGAA AACTTGCCGG CACGCCAACA TCGTGGCCTA CCATGGGAGT 
TATCTCTGGT TGCAGAAACT CTGGATCTGC ATGGAATTCT GTGGGGCTGG TTCTCTCCAG 
GACATCTACC AAGTGACAGG CTCCCTGTCA GAGCTCCAGA TTAGCTATGT CTGCCGGGAA 
GTGCTCCAGG GACTGGCCTA TTTGCACTCA CAGAAGAAGA TACACAGGGA CATCAAGGGA 
GCTAACATCC TCATCAATGA TGCTGGGGAG GTCAGATTGG CTGACTTTGG CATCTCGGCC 
CAGATTGGGG CTACACTGGC CAGACGCCTC TCTTTCATTG GGACACCCTA CTGGATGGCT 
CCGGAAGTGG CAGCTGTGGC CCTGAAGGGA GGATACAATG AGCTGTGTGA CATCTGGTCC 
CTGGGCATCA CGGCCATCGA ACTGGCCGAG CTACAGCCAC CGCTCTTTGA TGTGCACCCT 
CTCAGAGTTC TCTTCCTCAT GACCAAGAGT GGCTACCAGC CTCCCCGACT GAAGGAAAAA 
GGCAAATGGT CGGCTGCCTT CCACAACTTC ATCAAAGTCA CTCTGACTAA GAGTCCCAAG 
AAACGACCCA GCGCCACCAA GATGCTCAGT CATCAACTGG TATCCCAGCC TGGGCTGAAT 
CGAGGCCTGA TCCTGGATCT TCTTGACAAA CTGAAGAATC CCGGGAAAGG ACCCTCCATT 
GGGGACATTG AGGATGAGGA GCCCGAGCTA CCCCCTGCTA TCCCTCGGCG GATCAGATCC 
ACCCACCGCT CCAGCTCTCT GGGGATCCCA GATGCAGACT GCTGTCGGCG GCACATGGAG 
TTCAGGAAGC TCCGAGGAAT GGAGACCAGA CCCCCAGCCA ACACCGCTCG CCTACAGCCT 
CCTCGAGACC TCAGGAGCAG CAGCCCCAGG AAGCAACTGT CAGAGTCGTC TGACGATGAC 
TATGACGACG TGGACATCCC CACCCCTGCA GAGGACACAC CTCCTCCACT TCCCCCCAAG 
CCCAAGTTCC GTTCTCCATC AGACGAGGGT CCTGGGAGCA TGGGGGATGA TGGGCAGCTG 
AGCCCGGGGG TGCTGGTCCG GTGTGCCAGT GGGCCCCCAC CAAACAGCCC CCGTCCTGGG 
CCTCCCCCAT CCACCAGCAG CCCCCACCTC ACCGCCCATT CAGAACCCTC ACTCTGGAAC 
CCACCCTCCC GGGAGCTTGA CAAGCCCCCA CTTCTGCCCC CCAAGAAGGA AAAGATGAAG 
AGAAAGGGAT GTGCCCTTCT CGTAAAGTTG TTCAATGGCT GCCCCCTCCG GATCCACAGC 
ACGGCCGCCT GGACACATCC CTCCACCAAG GACCAGCACC TGCTCCTGGG GGCAGAGGAA 
GGCATCTTCA TCCTGAACCG GAATGACCAG GAGGCCACGC TGGAAATGCT CTTTCCTAGC 
CGGACTACGT GGGTGTACTC CATCAACAAC GTTCTCATGT CTCTCTCAGG AAAGACCCCC 
CACCTGTATT CTCATAGCAT CCTTGGCCTG CTGGAACGGA AAGAGACCAG AGCAGGAAAC 
CCCATCGCTC ACATTAGCCC CCACCGCCTA CTGGCAAGGA AGAACATGGT TTCCACCAAG 
ATCCAGGACA CCAAAGGCTG CCGGGCGTGC TGTGTGGCGG AGGGTGCGAG CTCTGGGGGC 
CCGTTCCTGT GCGGTGCATT GGAGACGTCC GTTGTCCTGC TTCAGTGGTA CCAGCCCATG 
AACAAATTCC TGCTTGTCCG GCAGGTGCTG TTCCCACTGC CGACGCCTCT GTCCGTGTTC 
GCGCTGCTGA CCGGGCCAGG CTCTGAGCTG CCCGCTGTGT GCATCGGCGT GAGCCCCGGG 
CGGCCGGGGA AGTCGGTGCT CTTCCACACG GTGCGCTTTG GCGCGCTCTC TTGCTGGCTG 
GGCGAGATGA GCACCGAGCA CAGGGGACCC GTGCAGGTGA CCCAGGTAGA GGAAGATATG 
GTGATGGTGT TGATGGATGG CTCTGTGAAG CTGGTGACCC CGGAGGGGTC CCCAGTCCGG 
GGACTTCGCA CACCTGAGAT CCCCATGACC GAAGCGGTGG AGGCCGTGGC TATGGTTGGA 
GGTCAGCTTC AGGCCTTCTG GAAGCATGGA GTGCAGGTGT GGGCTCTAGG CTCGGATCAG 
CTGCTACAGG AGCTGAGAGA CCCTACCCTC ACTTTCCGTC TGCTTGGCTC CCCCAGGCCT 
GTAGTGGTGG AGACACGCCC AGTGGATGAT CCTACTGCTC CCAGCAACCT CTACATCCAG 
GAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001036065.1
CDS	59..2524
Translation	MDVVDPDIFNRDPRDHYDLLQRLGGGTYGEVFKARDKVSGDLVALKMVKMEPDDDVSTLQKEILILKTCRHANIVAYHGSYLWLQKLWICMEFCGAGSLQDIYQVTGSLSELQISYVCREVLQGLAYLHSQKKIHRDIKGANILINDAGEVRLADFGISAQIGATLARRLSFIGTPYWMAPEVAAVALKGGYNELCDIWSLGITAIELAELQPPLFDVHPLRVLFLMTKSGYQPPRLKEKGKWSAAFHNFIKVTLTKSPKKRPSATKMLSHQLVSQPGLNRGLILDLLDKLKNPGKGPSIGDIEDEEPELPPAIPRRIRSTHRSSSLGIPDADCCRRHMEFRKLRGMETRPPANTARLQPPRDLRSSSPRKQLSESSDDDYDDVDIPTPAEDTPPPLPPKPKFRSPSDEGPGSMGDDGQLSPGVLVRCASGPPPNSPRPGPPPSTSSPHLTAHSEPSLWNPPSRELDKPPLLPPKKEKMKRKGCALLVKLFNGCPLRIHSTAAWTHPSTKDQHLLLGAEEGIFILNRNDQEATLEMLFPSRTTWVYSINNVLMSLSGKTPHLYSHSILGLLERKETRAGNPIAHISPHRLLARKNMVSTKIQDTKGCRACCVAEGASSGGPFLCGALETSVVLLQWYQPMNKFLLVRQVLFPLPTPLSVFALLTGPGSELPAVCIGVSPGRPGKSVLFHTVRFGALSCWLGEMSTEHRGPVQVTQVEEDMVMVLMDGSVKLVTPEGSPVRGLRTPEIPMTEAVEAVAMVGGQLQAFWKHGVQVWALGSDQLLQELRDPTLTFRLLGSPRPVVVETRPVDDPTAPSNLYIQE

Target ORF information:

RefSeq Version	NM_001042600.3
Organism	Homo sapiens(human)
Definition	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 1 (MAP4K1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001042600.3

ORF Insert Sequence:

ATGGACGTCG TGGACCCTGA CATTTTCAAT AGAGACCCCC GGGACCACTA TGACCTGCTA 
CAGCGGCTGG GTGGCGGCAC GTATGGGGAA GTCTTTAAGG CTCGAGACAA GGTGTCAGGG 
GACCTGGTGG CACTGAAGAT GGTGAAGATG GAGCCTGATG ATGATGTCTC CACCCTTCAG 
AAGGAAATCC TCATATTGAA AACTTGCCGG CACGCCAACA TCGTGGCCTA CCATGGGAGT 
TATCTCTGGT TGCAGAAACT CTGGATCTGC ATGGAATTCT GTGGGGCTGG TTCTCTCCAG 
GACATCTACC AAGTGACAGG CTCCCTGTCA GAGCTCCAGA TTAGCTATGT CTGCCGGGAA 
GTGCTCCAGG GACTGGCCTA TTTGCACTCA CAGAAGAAGA TACACAGGGA CATCAAGGGA 
GCTAACATCC TCATCAATGA TGCTGGGGAG GTCAGATTGG CTGACTTTGG CATCTCGGCC 
CAGATTGGGG CTACACTGGC CAGACGCCTC TCTTTCATTG GGACACCCTA CTGGATGGCT 
CCGGAAGTGG CAGCTGTGGC CCTGAAGGGA GGATACAATG AGCTGTGTGA CATCTGGTCC 
CTGGGCATCA CGGCCATCGA ACTGGCCGAG CTACAGCCAC CGCTCTTTGA TGTGCACCCT 
CTCAGAGTTC TCTTCCTCAT GACCAAGAGT GGCTACCAGC CTCCCCGACT GAAGGAAAAA 
GGCAAATGGT CGGCTGCCTT CCACAACTTC ATCAAAGTCA CTCTGACTAA GAGTCCCAAG 
AAACGACCCA GCGCCACCAA GATGCTCAGT CATCAACTGG TATCCCAGCC TGGGCTGAAT 
CGAGGCCTGA TCCTGGATCT TCTTGACAAA CTGAAGAATC CCGGGAAAGG ACCCTCCATT 
GGGGACATTG AGGATGAGGA GCCCGAGCTA CCCCCTGCTA TCCCTCGGCG GATCAGATCC 
ACCCACCGCT CCAGCTCTCT GGGGATCCCA GATGCAGACT GCTGTCGGCG GCACATGGAG 
TTCAGGAAGC TCCGAGGAAT GGAGACCAGA CCCCCAGCCA ACACCGCTCG CCTACAGCCT 
CCTCGAGACC TCAGGAGCAG CAGCCCCAGG AAGCAACTGT CAGAGTCGTC TGACGATGAC 
TATGACGACG TGGACATCCC CACCCCTGCA GAGGACACAC CTCCTCCACT TCCCCCCAAG 
CCCAAGTTCC GTTCTCCATC AGACGAGGGT CCTGGGAGCA TGGGGGATGA TGGGCAGCTG 
AGCCCGGGGG TGCTGGTCCG GTGTGCCAGT GGGCCCCCAC CAAACAGCCC CCGTCCTGGG 
CCTCCCCCAT CCACCAGCAG CCCCCACCTC ACCGCCCATT CAGAACCCTC ACTCTGGAAC 
CCACCCTCCC GGGAGCTTGA CAAGCCCCCA CTTCTGCCCC CCAAGAAGGA AAAGATGAAG 
AGAAAGGGAT GTGCCCTTCT CGTAAAGTTG TTCAATGGCT GCCCCCTCCG GATCCACAGC 
ACGGCCGCCT GGACACATCC CTCCACCAAG GACCAGCACC TGCTCCTGGG GGCAGAGGAA 
GGCATCTTCA TCCTGAACCG GAATGACCAG GAGGCCACGC TGGAAATGCT CTTTCCTAGC 
CGGACTACGT GGGTGTACTC CATCAACAAC GTTCTCATGT CTCTCTCAGG AAAGACCCCC 
CACCTGTATT CTCATAGCAT CCTTGGCCTG CTGGAACGGA AAGAGACCAG AGCAGGAAAC 
CCCATCGCTC ACATTAGCCC CCACCGCCTA CTGGCAAGGA AGAACATGGT TTCCACCAAG 
ATCCAGGACA CCAAAGGCTG CCGGGCGTGC TGTGTGGCGG AGGGTGCGAG CTCTGGGGGC 
CCGTTCCTGT GCGGTGCATT GGAGACGTCC GTTGTCCTGC TTCAGTGGTA CCAGCCCATG 
AACAAATTCC TGCTTGTCCG GCAGGTGCTG TTCCCACTGC CGACGCCTCT GTCCGTGTTC 
GCGCTGCTGA CCGGGCCAGG CTCTGAGCTG CCCGCTGTGT GCATCGGCGT GAGCCCCGGG 
CGGCCGGGGA AGTCGGTGCT CTTCCACACG GTGCGCTTTG GCGCGCTCTC TTGCTGGCTG 
GGCGAGATGA GCACCGAGCA CAGGGGACCC GTGCAGGTGA CCCAGGTAGA GGAAGATATG 
GTGATGGTGT TGATGGATGG CTCTGTGAAG CTGGTGACCC CGGAGGGGTC CCCAGTCCGG 
GGACTTCGCA CACCTGAGAT CCCCATGACC GAAGCGGTGG AGGCCGTGGC TATGGTTGGA 
GGTCAGCTTC AGGCCTTCTG GAAGCATGGA GTGCAGGTGT GGGCTCTAGG CTCGGATCAG 
CTGCTACAGG AGCTGAGAGA CCCTACCCTC ACTTTCCGTC TGCTTGGCTC CCCCAGGCCT 
GTAGTGGTGG AGACACGCCC AGTGGATGAT CCTACTGCTC CCAGCAACCT CTACATCCAG 
GAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP4K1 ( NM_007181.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_009112.1 Homo sapiens mitogen-activated protein kinase kinase kinase kinase 1 (MAP4K1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu21316
Clone ID Related Accession (Same CDS sequence)	NM_007181.5 , NM_007181.6
Accession Version	NM_007181.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2502bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Homo sapiens(human)
Product	mitogen-activated protein kinase kinase kinase kinase 1 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from HY074876.1, U66464.1 and AA995240.1. On Nov 16, 2013 this sequence version replaced NM_007181.4. Transcript Variant: This variant (2) represents the longer transcript and encodes the longer isoform (2). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U66464.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACGTCG TGGACCCTGA CATTTTCAAT AGAGACCCCC GGGACCACTA TGACCTGCTA 
CAGCGGCTGG GTGGCGGCAC GTATGGGGAA GTCTTTAAGG CTCGAGACAA GGTGTCAGGG 
GACCTGGTGG CACTGAAGAT GGTGAAGATG GAGCCTGATG ATGATGTCTC CACCCTTCAG 
AAGGAAATCC TCATATTGAA AACTTGCCGG CACGCCAACA TCGTGGCCTA CCATGGGAGT 
TATCTCTGGT TGCAGAAACT CTGGATCTGC ATGGAATTCT GTGGGGCTGG TTCTCTCCAG 
GACATCTACC AAGTGACAGG CTCCCTGTCA GAGCTCCAGA TTAGCTATGT CTGCCGGGAA 
GTGCTCCAGG GACTGGCCTA TTTGCACTCA CAGAAGAAGA TACACAGGGA CATCAAGGGA 
GCTAACATCC TCATCAATGA TGCTGGGGAG GTCAGATTGG CTGACTTTGG CATCTCGGCC 
CAGATTGGGG CTACACTGGC CAGACGCCTC TCTTTCATTG GGACACCCTA CTGGATGGCT 
CCGGAAGTGG CAGCTGTGGC CCTGAAGGGA GGATACAATG AGCTGTGTGA CATCTGGTCC 
CTGGGCATCA CGGCCATCGA ACTGGCCGAG CTACAGCCAC CGCTCTTTGA TGTGCACCCT 
CTCAGAGTTC TCTTCCTCAT GACCAAGAGT GGCTACCAGC CTCCCCGACT GAAGGAAAAA 
GGCAAATGGT CGGCTGCCTT CCACAACTTC ATCAAAGTCA CTCTGACTAA GAGTCCCAAG 
AAACGACCCA GCGCCACCAA GATGCTCAGT CATCAACTGG TATCCCAGCC TGGGCTGAAT 
CGAGGCCTGA TCCTGGATCT TCTTGACAAA CTGAAGAATC CCGGGAAAGG ACCCTCCATT 
GGGGACATTG AGGATGAGGA GCCCGAGCTA CCCCCTGCTA TCCCTCGGCG GATCAGATCC 
ACCCACCGCT CCAGCTCTCT GGGGATCCCA GATGCAGACT GCTGTCGGCG GCACATGGAG 
TTCAGGAAGC TCCGAGGAAT GGAGACCAGA CCCCCAGCCA ACACCGCTCG CCTACAGCCT 
CCTCGAGACC TCAGGAGCAG CAGCCCCAGG AAGCAACTGT CAGAGTCGTC TGACGATGAC 
TATGACGACG TGGACATCCC CACCCCTGCA GAGGACACAC CTCCTCCACT TCCCCCCAAG 
CCCAAGTTCC GTTCTCCATC AGACGAGGGT CCTGGGAGCA TGGGGGATGA TGGGCAGCTG 
AGCCCGGGGG TGCTGGTCCG GTGTGCCAGT GGGCCCCCAC CAAACAGCCC CCGTCCTGGG 
CCTCCCCCAT CCACCAGCAG CCCCCACCTC ACCGCCCATT CAGAACCCTC ACTCTGGAAC 
CCACCCTCCC GGGAGCTTGA CAAGCCCCCA CTTCTGCCCC CCAAGAAGGA AAAGATGAAG 
AGAAAGGGAT GTGCCCTTCT CGTAAAGTTG TTCAATGGCT GCCCCCTCCG GATCCACAGC 
ACGGCCGCCT GGACACATCC CTCCACCAAG GACCAGCACC TGCTCCTGGG GGCAGAGGAA 
GGCATCTTCA TCCTGAACCG GAATGACCAG GAGGCCACGC TGGAAATGCT CTTTCCTAGC 
CGGACTACGT GGGTGTACTC CATCAACAAC GTTCTCATGT CTCTCTCAGG AAAGACCCCC 
CACCTGTATT CTCATAGCAT CCTTGGCCTG CTGGAACGGA AAGAGACCAG AGCAGGAAAC 
CCCATCGCTC ACATTAGCCC CCACCGCCTA CTGGCAAGGA AGAACATGGT TTCCACCAAG 
ATCCAGGACA CCAAAGGCTG CCGGGCGTGC TGTGTGGCGG AGGGTGCGAG CTCTGGGGGC 
CCGTTCCTGT GCGGTGCATT GGAGACGTCC GTTGTCCTGC TTCAGTGGTA CCAGCCCATG 
AACAAATTCC TGCTTGTCCG GCAGGTGCTG TTCCCACTGC CGACGCCTCT GTCCGTGTTC 
GCGCTGCTGA CCGGGCCAGG CTCTGAGCTG CCCGCTGTGT GCATCGGCGT GAGCCCCGGG 
CGGCCGGGGA AGTCGGTGCT CTTCCACACG GTGCGCTTTG GCGCGCTCTC TTGCTGGCTG 
GGCGAGATGA GCACCGAGCA CAGGGGACCC GTGCAGGTGA CCCAGGTAGA GGAAGATATG 
GTGATGGTGT TGATGGATGG CTCTGTGAAG CTGGTGACCC CGGAGGGGTC CCCAGTCCGG 
GGACTTCGCA CACCTGAGAT CCCCATGACC GAAGCGGTGG AGGCCGTGGC TATGGTTGGA 
GGTCAGCTTC AGGCCTTCTG GAAGCATGGA GTGCAGGTGT GGGCTCTAGG CTCGGATCAG 
CTGCTACAGG AGCTGAGAGA CCCTACCCTC ACTTTCCGTC TGCTTGGCTC CCCCAGGCTG 
GAGTGCAGTG GCACGATCTC GCCTCACTGC AACCTCCTCC TCCCAGGTTC AAGCAATTCT 
CCTGCCTCAG CCTCCCGAGT AGCTGGGATT ACAGGCCTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_009112.1
CDS	141..2642
Translation	MDVVDPDIFNRDPRDHYDLLQRLGGGTYGEVFKARDKVSGDLVALKMVKMEPDDDVSTLQKEILILKTCRHANIVAYHGSYLWLQKLWICMEFCGAGSLQDIYQVTGSLSELQISYVCREVLQGLAYLHSQKKIHRDIKGANILINDAGEVRLADFGISAQIGATLARRLSFIGTPYWMAPEVAAVALKGGYNELCDIWSLGITAIELAELQPPLFDVHPLRVLFLMTKSGYQPPRLKEKGKWSAAFHNFIKVTLTKSPKKRPSATKMLSHQLVSQPGLNRGLILDLLDKLKNPGKGPSIGDIEDEEPELPPAIPRRIRSTHRSSSLGIPDADCCRRHMEFRKLRGMETRPPANTARLQPPRDLRSSSPRKQLSESSDDDYDDVDIPTPAEDTPPPLPPKPKFRSPSDEGPGSMGDDGQLSPGVLVRCASGPPPNSPRPGPPPSTSSPHLTAHSEPSLWNPPSRELDKPPLLPPKKEKMKRKGCALLVKLFNGCPLRIHSTAAWTHPSTKDQHLLLGAEEGIFILNRNDQEATLEMLFPSRTTWVYSINNVLMSLSGKTPHLYSHSILGLLERKETRAGNPIAHISPHRLLARKNMVSTKIQDTKGCRACCVAEGASSGGPFLCGALETSVVLLQWYQPMNKFLLVRQVLFPLPTPLSVFALLTGPGSELPAVCIGVSPGRPGKSVLFHTVRFGALSCWLGEMSTEHRGPVQVTQVEEDMVMVLMDGSVKLVTPEGSPVRGLRTPEIPMTEAVEAVAMVGGQLQAFWKHGVQVWALGSDQLLQELRDPTLTFRLLGSPRLECSGTISPHCNLLLPGSSNSPASASRVAGITGL

Target ORF information:

RefSeq Version	NM_007181.5
Organism	Homo sapiens(human)
Definition	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 1 (MAP4K1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007181.5

ORF Insert Sequence:

ATGGACGTCG TGGACCCTGA CATTTTCAAT AGAGACCCCC GGGACCACTA TGACCTGCTA 
CAGCGGCTGG GTGGCGGCAC GTATGGGGAA GTCTTTAAGG CTCGAGACAA GGTGTCAGGG 
GACCTGGTGG CACTGAAGAT GGTGAAGATG GAGCCTGATG ATGATGTCTC CACCCTTCAG 
AAGGAAATCC TCATATTGAA AACTTGCCGG CACGCCAACA TCGTGGCCTA CCATGGGAGT 
TATCTCTGGT TGCAGAAACT CTGGATCTGC ATGGAATTCT GTGGGGCTGG TTCTCTCCAG 
GACATCTACC AAGTGACAGG CTCCCTGTCA GAGCTCCAGA TTAGCTATGT CTGCCGGGAA 
GTGCTCCAGG GACTGGCCTA TTTGCACTCA CAGAAGAAGA TACACAGGGA CATCAAGGGA 
GCTAACATCC TCATCAATGA TGCTGGGGAG GTCAGATTGG CTGACTTTGG CATCTCGGCC 
CAGATTGGGG CTACACTGGC CAGACGCCTC TCTTTCATTG GGACACCCTA CTGGATGGCT 
CCGGAAGTGG CAGCTGTGGC CCTGAAGGGA GGATACAATG AGCTGTGTGA CATCTGGTCC 
CTGGGCATCA CGGCCATCGA ACTGGCCGAG CTACAGCCAC CGCTCTTTGA TGTGCACCCT 
CTCAGAGTTC TCTTCCTCAT GACCAAGAGT GGCTACCAGC CTCCCCGACT GAAGGAAAAA 
GGCAAATGGT CGGCTGCCTT CCACAACTTC ATCAAAGTCA CTCTGACTAA GAGTCCCAAG 
AAACGACCCA GCGCCACCAA GATGCTCAGT CATCAACTGG TATCCCAGCC TGGGCTGAAT 
CGAGGCCTGA TCCTGGATCT TCTTGACAAA CTGAAGAATC CCGGGAAAGG ACCCTCCATT 
GGGGACATTG AGGATGAGGA GCCCGAGCTA CCCCCTGCTA TCCCTCGGCG GATCAGATCC 
ACCCACCGCT CCAGCTCTCT GGGGATCCCA GATGCAGACT GCTGTCGGCG GCACATGGAG 
TTCAGGAAGC TCCGAGGAAT GGAGACCAGA CCCCCAGCCA ACACCGCTCG CCTACAGCCT 
CCTCGAGACC TCAGGAGCAG CAGCCCCAGG AAGCAACTGT CAGAGTCGTC TGACGATGAC 
TATGACGACG TGGACATCCC CACCCCTGCA GAGGACACAC CTCCTCCACT TCCCCCCAAG 
CCCAAGTTCC GTTCTCCATC AGACGAGGGT CCTGGGAGCA TGGGGGATGA TGGGCAGCTG 
AGCCCGGGGG TGCTGGTCCG GTGTGCCAGT GGGCCCCCAC CAAACAGCCC CCGTCCTGGG 
CCTCCCCCAT CCACCAGCAG CCCCCACCTC ACCGCCCATT CAGAACCCTC ACTCTGGAAC 
CCACCCTCCC GGGAGCTTGA CAAGCCCCCA CTTCTGCCCC CCAAGAAGGA AAAGATGAAG 
AGAAAGGGAT GTGCCCTTCT CGTAAAGTTG TTCAATGGCT GCCCCCTCCG GATCCACAGC 
ACGGCCGCCT GGACACATCC CTCCACCAAG GACCAGCACC TGCTCCTGGG GGCAGAGGAA 
GGCATCTTCA TCCTGAACCG GAATGACCAG GAGGCCACGC TGGAAATGCT CTTTCCTAGC 
CGGACTACGT GGGTGTACTC CATCAACAAC GTTCTCATGT CTCTCTCAGG AAAGACCCCC 
CACCTGTATT CTCATAGCAT CCTTGGCCTG CTGGAACGGA AAGAGACCAG AGCAGGAAAC 
CCCATCGCTC ACATTAGCCC CCACCGCCTA CTGGCAAGGA AGAACATGGT TTCCACCAAG 
ATCCAGGACA CCAAAGGCTG CCGGGCGTGC TGTGTGGCGG AGGGTGCGAG CTCTGGGGGC 
CCGTTCCTGT GCGGTGCATT GGAGACGTCC GTTGTCCTGC TTCAGTGGTA CCAGCCCATG 
AACAAATTCC TGCTTGTCCG GCAGGTGCTG TTCCCACTGC CGACGCCTCT GTCCGTGTTC 
GCGCTGCTGA CCGGGCCAGG CTCTGAGCTG CCCGCTGTGT GCATCGGCGT GAGCCCCGGG 
CGGCCGGGGA AGTCGGTGCT CTTCCACACG GTGCGCTTTG GCGCGCTCTC TTGCTGGCTG 
GGCGAGATGA GCACCGAGCA CAGGGGACCC GTGCAGGTGA CCCAGGTAGA GGAAGATATG 
GTGATGGTGT TGATGGATGG CTCTGTGAAG CTGGTGACCC CGGAGGGGTC CCCAGTCCGG 
GGACTTCGCA CACCTGAGAT CCCCATGACC GAAGCGGTGG AGGCCGTGGC TATGGTTGGA 
GGTCAGCTTC AGGCCTTCTG GAAGCATGGA GTGCAGGTGT GGGCTCTAGG CTCGGATCAG 
CTGCTACAGG AGCTGAGAGA CCCTACCCTC ACTTTCCGTC TGCTTGGCTC CCCCAGGCTG 
GAGTGCAGTG GCACGATCTC GCCTCACTGC AACCTCCTCC TCCCAGGTTC AAGCAATTCT 
CCTGCCTCAG CCTCCCGAGT AGCTGGGATT ACAGGCCTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP4K1 ( NM_007181.6 ) cDNA ORF clone, Homo sapiens(human) -> NP_009112.1 Homo sapiens mitogen-activated protein kinase kinase kinase kinase 1 (MAP4K1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu21316
Clone ID Related Accession (Same CDS sequence)	NM_007181.5 , NM_007181.6
Accession Version	NM_007181.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2502bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-30
Organism	Homo sapiens(human)
Product	mitogen-activated protein kinase kinase kinase kinase 1 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from HY074876.1 and U66464.1. On May 31, 2019 this sequence version replaced NM_007181.5. Transcript Variant: This variant (2) represents the longer transcript and encodes the longer isoform (2). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U66464.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACGTCG TGGACCCTGA CATTTTCAAT AGAGACCCCC GGGACCACTA TGACCTGCTA 
CAGCGGCTGG GTGGCGGCAC GTATGGGGAA GTCTTTAAGG CTCGAGACAA GGTGTCAGGG 
GACCTGGTGG CACTGAAGAT GGTGAAGATG GAGCCTGATG ATGATGTCTC CACCCTTCAG 
AAGGAAATCC TCATATTGAA AACTTGCCGG CACGCCAACA TCGTGGCCTA CCATGGGAGT 
TATCTCTGGT TGCAGAAACT CTGGATCTGC ATGGAATTCT GTGGGGCTGG TTCTCTCCAG 
GACATCTACC AAGTGACAGG CTCCCTGTCA GAGCTCCAGA TTAGCTATGT CTGCCGGGAA 
GTGCTCCAGG GACTGGCCTA TTTGCACTCA CAGAAGAAGA TACACAGGGA CATCAAGGGA 
GCTAACATCC TCATCAATGA TGCTGGGGAG GTCAGATTGG CTGACTTTGG CATCTCGGCC 
CAGATTGGGG CTACACTGGC CAGACGCCTC TCTTTCATTG GGACACCCTA CTGGATGGCT 
CCGGAAGTGG CAGCTGTGGC CCTGAAGGGA GGATACAATG AGCTGTGTGA CATCTGGTCC 
CTGGGCATCA CGGCCATCGA ACTGGCCGAG CTACAGCCAC CGCTCTTTGA TGTGCACCCT 
CTCAGAGTTC TCTTCCTCAT GACCAAGAGT GGCTACCAGC CTCCCCGACT GAAGGAAAAA 
GGCAAATGGT CGGCTGCCTT CCACAACTTC ATCAAAGTCA CTCTGACTAA GAGTCCCAAG 
AAACGACCCA GCGCCACCAA GATGCTCAGT CATCAACTGG TATCCCAGCC TGGGCTGAAT 
CGAGGCCTGA TCCTGGATCT TCTTGACAAA CTGAAGAATC CCGGGAAAGG ACCCTCCATT 
GGGGACATTG AGGATGAGGA GCCCGAGCTA CCCCCTGCTA TCCCTCGGCG GATCAGATCC 
ACCCACCGCT CCAGCTCTCT GGGGATCCCA GATGCAGACT GCTGTCGGCG GCACATGGAG 
TTCAGGAAGC TCCGAGGAAT GGAGACCAGA CCCCCAGCCA ACACCGCTCG CCTACAGCCT 
CCTCGAGACC TCAGGAGCAG CAGCCCCAGG AAGCAACTGT CAGAGTCGTC TGACGATGAC 
TATGACGACG TGGACATCCC CACCCCTGCA GAGGACACAC CTCCTCCACT TCCCCCCAAG 
CCCAAGTTCC GTTCTCCATC AGACGAGGGT CCTGGGAGCA TGGGGGATGA TGGGCAGCTG 
AGCCCGGGGG TGCTGGTCCG GTGTGCCAGT GGGCCCCCAC CAAACAGCCC CCGTCCTGGG 
CCTCCCCCAT CCACCAGCAG CCCCCACCTC ACCGCCCATT CAGAACCCTC ACTCTGGAAC 
CCACCCTCCC GGGAGCTTGA CAAGCCCCCA CTTCTGCCCC CCAAGAAGGA AAAGATGAAG 
AGAAAGGGAT GTGCCCTTCT CGTAAAGTTG TTCAATGGCT GCCCCCTCCG GATCCACAGC 
ACGGCCGCCT GGACACATCC CTCCACCAAG GACCAGCACC TGCTCCTGGG GGCAGAGGAA 
GGCATCTTCA TCCTGAACCG GAATGACCAG GAGGCCACGC TGGAAATGCT CTTTCCTAGC 
CGGACTACGT GGGTGTACTC CATCAACAAC GTTCTCATGT CTCTCTCAGG AAAGACCCCC 
CACCTGTATT CTCATAGCAT CCTTGGCCTG CTGGAACGGA AAGAGACCAG AGCAGGAAAC 
CCCATCGCTC ACATTAGCCC CCACCGCCTA CTGGCAAGGA AGAACATGGT TTCCACCAAG 
ATCCAGGACA CCAAAGGCTG CCGGGCGTGC TGTGTGGCGG AGGGTGCGAG CTCTGGGGGC 
CCGTTCCTGT GCGGTGCATT GGAGACGTCC GTTGTCCTGC TTCAGTGGTA CCAGCCCATG 
AACAAATTCC TGCTTGTCCG GCAGGTGCTG TTCCCACTGC CGACGCCTCT GTCCGTGTTC 
GCGCTGCTGA CCGGGCCAGG CTCTGAGCTG CCCGCTGTGT GCATCGGCGT GAGCCCCGGG 
CGGCCGGGGA AGTCGGTGCT CTTCCACACG GTGCGCTTTG GCGCGCTCTC TTGCTGGCTG 
GGCGAGATGA GCACCGAGCA CAGGGGACCC GTGCAGGTGA CCCAGGTAGA GGAAGATATG 
GTGATGGTGT TGATGGATGG CTCTGTGAAG CTGGTGACCC CGGAGGGGTC CCCAGTCCGG 
GGACTTCGCA CACCTGAGAT CCCCATGACC GAAGCGGTGG AGGCCGTGGC TATGGTTGGA 
GGTCAGCTTC AGGCCTTCTG GAAGCATGGA GTGCAGGTGT GGGCTCTAGG CTCGGATCAG 
CTGCTACAGG AGCTGAGAGA CCCTACCCTC ACTTTCCGTC TGCTTGGCTC CCCCAGGCTG 
GAGTGCAGTG GCACGATCTC GCCTCACTGC AACCTCCTCC TCCCAGGTTC AAGCAATTCT 
CCTGCCTCAG CCTCCCGAGT AGCTGGGATT ACAGGCCTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_009112.1
CDS	59..2560
Translation	MDVVDPDIFNRDPRDHYDLLQRLGGGTYGEVFKARDKVSGDLVALKMVKMEPDDDVSTLQKEILILKTCRHANIVAYHGSYLWLQKLWICMEFCGAGSLQDIYQVTGSLSELQISYVCREVLQGLAYLHSQKKIHRDIKGANILINDAGEVRLADFGISAQIGATLARRLSFIGTPYWMAPEVAAVALKGGYNELCDIWSLGITAIELAELQPPLFDVHPLRVLFLMTKSGYQPPRLKEKGKWSAAFHNFIKVTLTKSPKKRPSATKMLSHQLVSQPGLNRGLILDLLDKLKNPGKGPSIGDIEDEEPELPPAIPRRIRSTHRSSSLGIPDADCCRRHMEFRKLRGMETRPPANTARLQPPRDLRSSSPRKQLSESSDDDYDDVDIPTPAEDTPPPLPPKPKFRSPSDEGPGSMGDDGQLSPGVLVRCASGPPPNSPRPGPPPSTSSPHLTAHSEPSLWNPPSRELDKPPLLPPKKEKMKRKGCALLVKLFNGCPLRIHSTAAWTHPSTKDQHLLLGAEEGIFILNRNDQEATLEMLFPSRTTWVYSINNVLMSLSGKTPHLYSHSILGLLERKETRAGNPIAHISPHRLLARKNMVSTKIQDTKGCRACCVAEGASSGGPFLCGALETSVVLLQWYQPMNKFLLVRQVLFPLPTPLSVFALLTGPGSELPAVCIGVSPGRPGKSVLFHTVRFGALSCWLGEMSTEHRGPVQVTQVEEDMVMVLMDGSVKLVTPEGSPVRGLRTPEIPMTEAVEAVAMVGGQLQAFWKHGVQVWALGSDQLLQELRDPTLTFRLLGSPRLECSGTISPHCNLLLPGSSNSPASASRVAGITGL

Target ORF information:

RefSeq Version	NM_007181.6
Organism	Homo sapiens(human)
Definition	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 1 (MAP4K1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007181.6

ORF Insert Sequence:

ATGGACGTCG TGGACCCTGA CATTTTCAAT AGAGACCCCC GGGACCACTA TGACCTGCTA 
CAGCGGCTGG GTGGCGGCAC GTATGGGGAA GTCTTTAAGG CTCGAGACAA GGTGTCAGGG 
GACCTGGTGG CACTGAAGAT GGTGAAGATG GAGCCTGATG ATGATGTCTC CACCCTTCAG 
AAGGAAATCC TCATATTGAA AACTTGCCGG CACGCCAACA TCGTGGCCTA CCATGGGAGT 
TATCTCTGGT TGCAGAAACT CTGGATCTGC ATGGAATTCT GTGGGGCTGG TTCTCTCCAG 
GACATCTACC AAGTGACAGG CTCCCTGTCA GAGCTCCAGA TTAGCTATGT CTGCCGGGAA 
GTGCTCCAGG GACTGGCCTA TTTGCACTCA CAGAAGAAGA TACACAGGGA CATCAAGGGA 
GCTAACATCC TCATCAATGA TGCTGGGGAG GTCAGATTGG CTGACTTTGG CATCTCGGCC 
CAGATTGGGG CTACACTGGC CAGACGCCTC TCTTTCATTG GGACACCCTA CTGGATGGCT 
CCGGAAGTGG CAGCTGTGGC CCTGAAGGGA GGATACAATG AGCTGTGTGA CATCTGGTCC 
CTGGGCATCA CGGCCATCGA ACTGGCCGAG CTACAGCCAC CGCTCTTTGA TGTGCACCCT 
CTCAGAGTTC TCTTCCTCAT GACCAAGAGT GGCTACCAGC CTCCCCGACT GAAGGAAAAA 
GGCAAATGGT CGGCTGCCTT CCACAACTTC ATCAAAGTCA CTCTGACTAA GAGTCCCAAG 
AAACGACCCA GCGCCACCAA GATGCTCAGT CATCAACTGG TATCCCAGCC TGGGCTGAAT 
CGAGGCCTGA TCCTGGATCT TCTTGACAAA CTGAAGAATC CCGGGAAAGG ACCCTCCATT 
GGGGACATTG AGGATGAGGA GCCCGAGCTA CCCCCTGCTA TCCCTCGGCG GATCAGATCC 
ACCCACCGCT CCAGCTCTCT GGGGATCCCA GATGCAGACT GCTGTCGGCG GCACATGGAG 
TTCAGGAAGC TCCGAGGAAT GGAGACCAGA CCCCCAGCCA ACACCGCTCG CCTACAGCCT 
CCTCGAGACC TCAGGAGCAG CAGCCCCAGG AAGCAACTGT CAGAGTCGTC TGACGATGAC 
TATGACGACG TGGACATCCC CACCCCTGCA GAGGACACAC CTCCTCCACT TCCCCCCAAG 
CCCAAGTTCC GTTCTCCATC AGACGAGGGT CCTGGGAGCA TGGGGGATGA TGGGCAGCTG 
AGCCCGGGGG TGCTGGTCCG GTGTGCCAGT GGGCCCCCAC CAAACAGCCC CCGTCCTGGG 
CCTCCCCCAT CCACCAGCAG CCCCCACCTC ACCGCCCATT CAGAACCCTC ACTCTGGAAC 
CCACCCTCCC GGGAGCTTGA CAAGCCCCCA CTTCTGCCCC CCAAGAAGGA AAAGATGAAG 
AGAAAGGGAT GTGCCCTTCT CGTAAAGTTG TTCAATGGCT GCCCCCTCCG GATCCACAGC 
ACGGCCGCCT GGACACATCC CTCCACCAAG GACCAGCACC TGCTCCTGGG GGCAGAGGAA 
GGCATCTTCA TCCTGAACCG GAATGACCAG GAGGCCACGC TGGAAATGCT CTTTCCTAGC 
CGGACTACGT GGGTGTACTC CATCAACAAC GTTCTCATGT CTCTCTCAGG AAAGACCCCC 
CACCTGTATT CTCATAGCAT CCTTGGCCTG CTGGAACGGA AAGAGACCAG AGCAGGAAAC 
CCCATCGCTC ACATTAGCCC CCACCGCCTA CTGGCAAGGA AGAACATGGT TTCCACCAAG 
ATCCAGGACA CCAAAGGCTG CCGGGCGTGC TGTGTGGCGG AGGGTGCGAG CTCTGGGGGC 
CCGTTCCTGT GCGGTGCATT GGAGACGTCC GTTGTCCTGC TTCAGTGGTA CCAGCCCATG 
AACAAATTCC TGCTTGTCCG GCAGGTGCTG TTCCCACTGC CGACGCCTCT GTCCGTGTTC 
GCGCTGCTGA CCGGGCCAGG CTCTGAGCTG CCCGCTGTGT GCATCGGCGT GAGCCCCGGG 
CGGCCGGGGA AGTCGGTGCT CTTCCACACG GTGCGCTTTG GCGCGCTCTC TTGCTGGCTG 
GGCGAGATGA GCACCGAGCA CAGGGGACCC GTGCAGGTGA CCCAGGTAGA GGAAGATATG 
GTGATGGTGT TGATGGATGG CTCTGTGAAG CTGGTGACCC CGGAGGGGTC CCCAGTCCGG 
GGACTTCGCA CACCTGAGAT CCCCATGACC GAAGCGGTGG AGGCCGTGGC TATGGTTGGA 
GGTCAGCTTC AGGCCTTCTG GAAGCATGGA GTGCAGGTGT GGGCTCTAGG CTCGGATCAG 
CTGCTACAGG AGCTGAGAGA CCCTACCCTC ACTTTCCGTC TGCTTGGCTC CCCCAGGCTG 
GAGTGCAGTG GCACGATCTC GCCTCACTGC AACCTCCTCC TCCCAGGTTC AAGCAATTCT 
CCTGCCTCAG CCTCCCGAGT AGCTGGGATT ACAGGCCTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP4K1 ( XM_011526403.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011524705.1 Homo sapiens mitogen-activated protein kinase kinase kinase kinase 1 (MAP4K1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu64825
Clone ID Related Accession (Same CDS sequence)	XM_011526403.1
Accession Version	XM_011526403.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2622bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	mitogen-activated protein kinase kinase kinase kinase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGTCG TGGACCCTGA CATTTTCAAT AGAGACCCCC GGGACCACTA TGACCTGCTA 
CAGCGGCTGG GTGGCGGCAC GTATGGGGAA GTCTTTAAGG CTCGAGACAA GGTGTCAGGG 
GACCTGGTGG CACTGAAGAT GGTGAAGATG GAGCCTGATG ATGATGTCTC CACCCTTCAG 
AAGGAAATCC TCATATTGAA AACTTGCCGG CACGCCAACA TCGTGGCCTA CCATGGGAGT 
TATCTCTGGT TGCAGAAACT CTGGATCTGC ATGGAATTCT GTGGGGCTGG TTCTCTCCAG 
GACATCTACC AAGTGACAGG CTCCCTGTCA GAGCTCCAGA TTAGCTATGT CTGCCGGGAA 
GTGCTCCAGG GACTGGCCTA TTTGCACTCA CAGAAGAAGA TACACAGGGA CATCAAGGGA 
GCTAACATCC TCATCAATGA TGCTGGGGAG GTCAGATTGG CTGACTTTGG CATCTCGGCC 
CAGATTGGGG CTACACTGGC CAGACGCCTC TCTTTCATTG GGACACCCTA CTGGATGGCT 
CCGGAAGTGG CAGCTGTGGC CCTGAAGGGA GGATACAATG AGCTGTGTGA CATCTGGTCC 
CTGGGCATCA CGGCCATCGA ACTGGCCGAG CTACAGCCAC CGCTCTTTGA TGTGCACCCT 
CTCAGAGTTC TCTTCCTCAT GACCAAGAGT GGCTACCAGC CTCCCCGACT GAAGGAAAAA 
GGCAAATGGT CGGCTGCCTT CCACAACTTC ATCAAAGTCA CTCTGACTAA GAGTCCCAAG 
AAACGACCCA GCGCCACCAA GATGCTCAGT CATCAACTGG TATCCCAGCC TGGGCTGAAT 
CGAGGCCTGA TCCTGGATCT TCTTGACAAA CTGAAGAATC CCGGGAAAGG ACCCTCCATT 
GGGGACATTG AGGATGAGGA GCCCGAGCTA CCCCCTGCTA TCCCTCGGCG GATCAGATCC 
ACCCACCGCT CCAGCTCTCT GGGGATCCCA GATGCAGACT GCTGTCGGCG GCACATGGAG 
TTCAGGAAGC TCCGAGGAAT GGAGACCAGA CCCCCAGCCA ACACCGCTCG CCTACAGCCT 
CCTCGAGACC TCAGGAGCAG CAGCCCCAGG AAGCAACTGT CAGAGTCGTC TGACGATGAC 
TATGACGACG TGGACATGCT GAACCTTGGC ACCTGGAAAT CTCCCAAAGA CCAGATACTG 
AGCCCCAGAT CCAGACCCAA CAGTCAGCCC AGGCCCCCAT ACTCCCCAGC CCACAGGCCG 
AGTCAGGCTC CATCCAGCCC CACCCCTGCA GAGGACACAC CTCCTCCACT TCCCCCCAAG 
CCCAAGTTCC GTTCTCCATC AGACGAGGGT CCTGGGAGCA TGGGGGATGA TGGGCAGCTG 
AGCCCGGGGG TGCTGGTCCG GTGTGCCAGT GGGCCCCCAC CAAACAGCCC CCGTCCTGGG 
CCTCCCCCAT CCACCAGCAG CCCCCACCTC ACCGCCCATT CAGAACCCTC ACTCTGGAAC 
CCACCCTCCC GGGAGCTTGA CAAGCCCCCA CTTCTGCCCC CCAAGAAGGA AAAGATGAAG 
AGAAAGGGAT GTGCCCTTCT CGTAAAGTTG TTCAATGGCT GCCCCCTCCG GATCCACAGC 
ACGGCCGCCT GGACACATCC CTCCACCAAG GACCAGCACC TGCTCCTGGG GGCAGAGGAA 
GGCATCTTCA TCCTGAACCG GAATGACCAG GAGGCCACGC TGGAAATGCT CTTTCCTAGC 
CGGACTACGT GGGTGTACTC CATCAACAAC GTTCTCATGT CTCTCTCAGG AAAGACCCCC 
CACCTGTATT CTCATAGCAT CCTTGGCCTG CTGGAACGGA AAGAGACCAG AGCAGGAAAC 
CCCATCGCTC ACATTAGCCC CCACCGCCTA CTGGCAAGGA AGAACATGGT TTCCACCAAG 
ATCCAGGACA CCAAAGGCTG CCGGGCGTGC TGTGTGGCGG AGGGTGCGAG CTCTGGGGGC 
CCGTTCCTGT GCGGTGCATT GGAGACGTCC GTTGTCCTGC TTCAGTGGTA CCAGCCCATG 
AACAAATTCC TGCTTGTCCG GCAGGTGCTG TTCCCACTGC CGACGCCTCT GTCCGTGTTC 
GCGCTGCTGA CCGGGCCAGG CTCTGAGCTG CCCGCTGTGT GCATCGGCGT GAGCCCCGGG 
CGGCCGGGGA AGTCGGTGCT CTTCCACACG GTGCGCTTTG GCGCGCTCTC TTGCTGGCTG 
GGCGAGATGA GCACCGAGCA CAGGGGACCC GTGCAGGTGA CCCAGGTAGA GGAAGATATG 
GTGATGGTGT TGATGGATGG CTCTGTGAAG CTGGTGACCC CGGAGGGGTC CCCAGTCCGG 
GGACTTCGCA CACCTGAGAT CCCCATGACC GAAGCGGTGG AGGCCGTGGC TATGGTTGGA 
GGTCAGCTTC AGGCCTTCTG GAAGCATGGA GTGCAGGTGT GGGCTCTAGG CTCGGATCAG 
CTGCTACAGG AGCTGAGAGA CCCTACCCTC ACTTTCCGTC TGCTTGGCTC CCCCAGGCTG 
GAGTGCAGTG GCACGATCTC GCCTCACTGC AACCTCCTCC TCCCAGGTTC AAGCAATTCT 
CCTGCCTCAG CCTCCCGAGT AGCTGGGATT ACAGGCCTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011524705.1
CDS	39..2660
Translation	MDVVDPDIFNRDPRDHYDLLQRLGGGTYGEVFKARDKVSGDLVALKMVKMEPDDDVSTLQKEILILKTCRHANIVAYHGSYLWLQKLWICMEFCGAGSLQDIYQVTGSLSELQISYVCREVLQGLAYLHSQKKIHRDIKGANILINDAGEVRLADFGISAQIGATLARRLSFIGTPYWMAPEVAAVALKGGYNELCDIWSLGITAIELAELQPPLFDVHPLRVLFLMTKSGYQPPRLKEKGKWSAAFHNFIKVTLTKSPKKRPSATKMLSHQLVSQPGLNRGLILDLLDKLKNPGKGPSIGDIEDEEPELPPAIPRRIRSTHRSSSLGIPDADCCRRHMEFRKLRGMETRPPANTARLQPPRDLRSSSPRKQLSESSDDDYDDVDMLNLGTWKSPKDQILSPRSRPNSQPRPPYSPAHRPSQAPSSPTPAEDTPPPLPPKPKFRSPSDEGPGSMGDDGQLSPGVLVRCASGPPPNSPRPGPPPSTSSPHLTAHSEPSLWNPPSRELDKPPLLPPKKEKMKRKGCALLVKLFNGCPLRIHSTAAWTHPSTKDQHLLLGAEEGIFILNRNDQEATLEMLFPSRTTWVYSINNVLMSLSGKTPHLYSHSILGLLERKETRAGNPIAHISPHRLLARKNMVSTKIQDTKGCRACCVAEGASSGGPFLCGALETSVVLLQWYQPMNKFLLVRQVLFPLPTPLSVFALLTGPGSELPAVCIGVSPGRPGKSVLFHTVRFGALSCWLGEMSTEHRGPVQVTQVEEDMVMVLMDGSVKLVTPEGSPVRGLRTPEIPMTEAVEAVAMVGGQLQAFWKHGVQVWALGSDQLLQELRDPTLTFRLLGSPRLECSGTISPHCNLLLPGSSNSPASASRVAGITGL

Target ORF information:

RefSeq Version	XM_011526403.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 1 (MAP4K1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011526403.1

ORF Insert Sequence:

ATGGACGTCG TGGACCCTGA CATTTTCAAT AGAGACCCCC GGGACCACTA TGACCTGCTA 
CAGCGGCTGG GTGGCGGCAC GTATGGGGAA GTCTTTAAGG CTCGAGACAA GGTGTCAGGG 
GACCTGGTGG CACTGAAGAT GGTGAAGATG GAGCCTGATG ATGATGTCTC CACCCTTCAG 
AAGGAAATCC TCATATTGAA AACTTGCCGG CACGCCAACA TCGTGGCCTA CCATGGGAGT 
TATCTCTGGT TGCAGAAACT CTGGATCTGC ATGGAATTCT GTGGGGCTGG TTCTCTCCAG 
GACATCTACC AAGTGACAGG CTCCCTGTCA GAGCTCCAGA TTAGCTATGT CTGCCGGGAA 
GTGCTCCAGG GACTGGCCTA TTTGCACTCA CAGAAGAAGA TACACAGGGA CATCAAGGGA 
GCTAACATCC TCATCAATGA TGCTGGGGAG GTCAGATTGG CTGACTTTGG CATCTCGGCC 
CAGATTGGGG CTACACTGGC CAGACGCCTC TCTTTCATTG GGACACCCTA CTGGATGGCT 
CCGGAAGTGG CAGCTGTGGC CCTGAAGGGA GGATACAATG AGCTGTGTGA CATCTGGTCC 
CTGGGCATCA CGGCCATCGA ACTGGCCGAG CTACAGCCAC CGCTCTTTGA TGTGCACCCT 
CTCAGAGTTC TCTTCCTCAT GACCAAGAGT GGCTACCAGC CTCCCCGACT GAAGGAAAAA 
GGCAAATGGT CGGCTGCCTT CCACAACTTC ATCAAAGTCA CTCTGACTAA GAGTCCCAAG 
AAACGACCCA GCGCCACCAA GATGCTCAGT CATCAACTGG TATCCCAGCC TGGGCTGAAT 
CGAGGCCTGA TCCTGGATCT TCTTGACAAA CTGAAGAATC CCGGGAAAGG ACCCTCCATT 
GGGGACATTG AGGATGAGGA GCCCGAGCTA CCCCCTGCTA TCCCTCGGCG GATCAGATCC 
ACCCACCGCT CCAGCTCTCT GGGGATCCCA GATGCAGACT GCTGTCGGCG GCACATGGAG 
TTCAGGAAGC TCCGAGGAAT GGAGACCAGA CCCCCAGCCA ACACCGCTCG CCTACAGCCT 
CCTCGAGACC TCAGGAGCAG CAGCCCCAGG AAGCAACTGT CAGAGTCGTC TGACGATGAC 
TATGACGACG TGGACATGCT GAACCTTGGC ACCTGGAAAT CTCCCAAAGA CCAGATACTG 
AGCCCCAGAT CCAGACCCAA CAGTCAGCCC AGGCCCCCAT ACTCCCCAGC CCACAGGCCG 
AGTCAGGCTC CATCCAGCCC CACCCCTGCA GAGGACACAC CTCCTCCACT TCCCCCCAAG 
CCCAAGTTCC GTTCTCCATC AGACGAGGGT CCTGGGAGCA TGGGGGATGA TGGGCAGCTG 
AGCCCGGGGG TGCTGGTCCG GTGTGCCAGT GGGCCCCCAC CAAACAGCCC CCGTCCTGGG 
CCTCCCCCAT CCACCAGCAG CCCCCACCTC ACCGCCCATT CAGAACCCTC ACTCTGGAAC 
CCACCCTCCC GGGAGCTTGA CAAGCCCCCA CTTCTGCCCC CCAAGAAGGA AAAGATGAAG 
AGAAAGGGAT GTGCCCTTCT CGTAAAGTTG TTCAATGGCT GCCCCCTCCG GATCCACAGC 
ACGGCCGCCT GGACACATCC CTCCACCAAG GACCAGCACC TGCTCCTGGG GGCAGAGGAA 
GGCATCTTCA TCCTGAACCG GAATGACCAG GAGGCCACGC TGGAAATGCT CTTTCCTAGC 
CGGACTACGT GGGTGTACTC CATCAACAAC GTTCTCATGT CTCTCTCAGG AAAGACCCCC 
CACCTGTATT CTCATAGCAT CCTTGGCCTG CTGGAACGGA AAGAGACCAG AGCAGGAAAC 
CCCATCGCTC ACATTAGCCC CCACCGCCTA CTGGCAAGGA AGAACATGGT TTCCACCAAG 
ATCCAGGACA CCAAAGGCTG CCGGGCGTGC TGTGTGGCGG AGGGTGCGAG CTCTGGGGGC 
CCGTTCCTGT GCGGTGCATT GGAGACGTCC GTTGTCCTGC TTCAGTGGTA CCAGCCCATG 
AACAAATTCC TGCTTGTCCG GCAGGTGCTG TTCCCACTGC CGACGCCTCT GTCCGTGTTC 
GCGCTGCTGA CCGGGCCAGG CTCTGAGCTG CCCGCTGTGT GCATCGGCGT GAGCCCCGGG 
CGGCCGGGGA AGTCGGTGCT CTTCCACACG GTGCGCTTTG GCGCGCTCTC TTGCTGGCTG 
GGCGAGATGA GCACCGAGCA CAGGGGACCC GTGCAGGTGA CCCAGGTAGA GGAAGATATG 
GTGATGGTGT TGATGGATGG CTCTGTGAAG CTGGTGACCC CGGAGGGGTC CCCAGTCCGG 
GGACTTCGCA CACCTGAGAT CCCCATGACC GAAGCGGTGG AGGCCGTGGC TATGGTTGGA 
GGTCAGCTTC AGGCCTTCTG GAAGCATGGA GTGCAGGTGT GGGCTCTAGG CTCGGATCAG 
CTGCTACAGG AGCTGAGAGA CCCTACCCTC ACTTTCCGTC TGCTTGGCTC CCCCAGGCTG 
GAGTGCAGTG GCACGATCTC GCCTCACTGC AACCTCCTCC TCCCAGGTTC AAGCAATTCT 
CCTGCCTCAG CCTCCCGAGT AGCTGGGATT ACAGGCCTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP4K1 ( XM_011526404.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011524706.1 Homo sapiens mitogen-activated protein kinase kinase kinase kinase 1 (MAP4K1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu64826
Clone ID Related Accession (Same CDS sequence)	XM_011526404.1
Accession Version	XM_011526404.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2586bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	mitogen-activated protein kinase kinase kinase kinase 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGTCG TGGACCCTGA CATTTTCAAT AGAGACCCCC GGGACCACTA TGACCTGCTA 
CAGCGGCTGG GTGGCGGCAC GTATGGGGAA GTCTTTAAGG CTCGAGACAA GGTGTCAGGG 
GACCTGGTGG CACTGAAGAT GGTGAAGATG GAGCCTGATG ATGATGTCTC CACCCTTCAG 
AAGGAAATCC TCATATTGAA AACTTGCCGG CACGCCAACA TCGTGGCCTA CCATGGGAGT 
TATCTCTGGT TGCAGAAACT CTGGATCTGC ATGGAATTCT GTGGGGCTGG TTCTCTCCAG 
GACATCTACC AAGTGACAGG CTCCCTGTCA GAGCTCCAGA TTAGCTATGT CTGCCGGGAA 
GTGCTCCAGG GACTGGCCTA TTTGCACTCA CAGAAGAAGA TACACAGGGA CATCAAGGGA 
GCTAACATCC TCATCAATGA TGCTGGGGAG GTCAGATTGG CTGACTTTGG CATCTCGGCC 
CAGATTGGGG CTACACTGGC CAGACGCCTC TCTTTCATTG GGACACCCTA CTGGATGGCT 
CCGGAAGTGG CAGCTGTGGC CCTGAAGGGA GGATACAATG AGCTGTGTGA CATCTGGTCC 
CTGGGCATCA CGGCCATCGA ACTGGCCGAG CTACAGCCAC CGCTCTTTGA TGTGCACCCT 
CTCAGAGTTC TCTTCCTCAT GACCAAGAGT GGCTACCAGC CTCCCCGACT GAAGGAAAAA 
GGCAAATGGT CGGCTGCCTT CCACAACTTC ATCAAAGTCA CTCTGACTAA GAGTCCCAAG 
AAACGACCCA GCGCCACCAA GATGCTCAGT CATCAACTGG TATCCCAGCC TGGGCTGAAT 
CGAGGCCTGA TCCTGGATCT TCTTGACAAA CTGAAGAATC CCGGGAAAGG ACCCTCCATT 
GGGGACATTG AGGATGAGGA GCCCGAGCTA CCCCCTGCTA TCCCTCGGCG GATCAGATCC 
ACCCACCGCT CCAGCTCTCT GGGGATCCCA GATGCAGACT GCTGTCGGCG GCACATGGAG 
TTCAGGAAGC TCCGAGGAAT GGAGACCAGA CCCCCAGCCA ACACCGCTCG CCTACAGCCT 
CCTCGAGACC TCAGGAGCAG CAGCCCCAGG AAGCAACTGT CAGAGTCGTC TGACGATGAC 
TATGACGACG TGGACATGCT GAACCTTGGC ACCTGGAAAT CTCCCAAAGA CCAGATACTG 
AGCCCCAGAT CCAGACCCAA CAGTCAGCCC AGGCCCCCAT ACTCCCCAGC CCACAGGCCG 
AGTCAGGCTC CATCCAGCCC CACCCCTGCA GAGGACACAC CTCCTCCACT TCCCCCCAAG 
CCCAAGTTCC GTTCTCCATC AGACGAGGGT CCTGGGAGCA TGGGGGATGA TGGGCAGCTG 
AGCCCGGGGG TGCTGGTCCG GTGTGCCAGT GGGCCCCCAC CAAACAGCCC CCGTCCTGGG 
CCTCCCCCAT CCACCAGCAG CCCCCACCTC ACCGCCCATT CAGAACCCTC ACTCTGGAAC 
CCACCCTCCC GGGAGCTTGA CAAGCCCCCA CTTCTGCCCC CCAAGAAGGA AAAGATGAAG 
AGAAAGGGAT GTGCCCTTCT CGTAAAGTTG TTCAATGGCT GCCCCCTCCG GATCCACAGC 
ACGGCCGCCT GGACACATCC CTCCACCAAG GACCAGCACC TGCTCCTGGG GGCAGAGGAA 
GGCATCTTCA TCCTGAACCG GAATGACCAG GAGGCCACGC TGGAAATGCT CTTTCCTAGC 
CGGACTACGT GGGTGTACTC CATCAACAAC GTTCTCATGT CTCTCTCAGG AAAGACCCCC 
CACCTGTATT CTCATAGCAT CCTTGGCCTG CTGGAACGGA AAGAGACCAG AGCAGGAAAC 
CCCATCGCTC ACATTAGCCC CCACCGCCTA CTGGCAAGGA AGAACATGGT TTCCACCAAG 
ATCCAGGACA CCAAAGGCTG CCGGGCGTGC TGTGTGGCGG AGGGTGCGAG CTCTGGGGGC 
CCGTTCCTGT GCGGTGCATT GGAGACGTCC GTTGTCCTGC TTCAGTGGTA CCAGCCCATG 
AACAAATTCC TGCTTGTCCG GCAGGTGCTG TTCCCACTGC CGACGCCTCT GTCCGTGTTC 
GCGCTGCTGA CCGGGCCAGG CTCTGAGCTG CCCGCTGTGT GCATCGGCGT GAGCCCCGGG 
CGGCCGGGGA AGTCGGTGCT CTTCCACACG GTGCGCTTTG GCGCGCTCTC TTGCTGGCTG 
GGCGAGATGA GCACCGAGCA CAGGGGACCC GTGCAGGTGA CCCAGGTAGA GGAAGATATG 
GTGATGGTGT TGATGGATGG CTCTGTGAAG CTGGTGACCC CGGAGGGGTC CCCAGTCCGG 
GGACTTCGCA CACCTGAGAT CCCCATGACC GAAGCGGTGG AGGCCGTGGC TATGGTTGGA 
GGTCAGCTTC AGGCCTTCTG GAAGCATGGA GTGCAGGTGT GGGCTCTAGG CTCGGATCAG 
CTGCTACAGG AGCTGAGAGA CCCTACCCTC ACTTTCCGTC TGCTTGGCTC CCCCAGGCCT 
GTAGTGGTGG AGACACGCCC AGTGGATGAT CCTACTGCTC CCAGCAACCT CTACATCCAG 
GAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011524706.1
CDS	39..2624
Translation	MDVVDPDIFNRDPRDHYDLLQRLGGGTYGEVFKARDKVSGDLVALKMVKMEPDDDVSTLQKEILILKTCRHANIVAYHGSYLWLQKLWICMEFCGAGSLQDIYQVTGSLSELQISYVCREVLQGLAYLHSQKKIHRDIKGANILINDAGEVRLADFGISAQIGATLARRLSFIGTPYWMAPEVAAVALKGGYNELCDIWSLGITAIELAELQPPLFDVHPLRVLFLMTKSGYQPPRLKEKGKWSAAFHNFIKVTLTKSPKKRPSATKMLSHQLVSQPGLNRGLILDLLDKLKNPGKGPSIGDIEDEEPELPPAIPRRIRSTHRSSSLGIPDADCCRRHMEFRKLRGMETRPPANTARLQPPRDLRSSSPRKQLSESSDDDYDDVDMLNLGTWKSPKDQILSPRSRPNSQPRPPYSPAHRPSQAPSSPTPAEDTPPPLPPKPKFRSPSDEGPGSMGDDGQLSPGVLVRCASGPPPNSPRPGPPPSTSSPHLTAHSEPSLWNPPSRELDKPPLLPPKKEKMKRKGCALLVKLFNGCPLRIHSTAAWTHPSTKDQHLLLGAEEGIFILNRNDQEATLEMLFPSRTTWVYSINNVLMSLSGKTPHLYSHSILGLLERKETRAGNPIAHISPHRLLARKNMVSTKIQDTKGCRACCVAEGASSGGPFLCGALETSVVLLQWYQPMNKFLLVRQVLFPLPTPLSVFALLTGPGSELPAVCIGVSPGRPGKSVLFHTVRFGALSCWLGEMSTEHRGPVQVTQVEEDMVMVLMDGSVKLVTPEGSPVRGLRTPEIPMTEAVEAVAMVGGQLQAFWKHGVQVWALGSDQLLQELRDPTLTFRLLGSPRPVVVETRPVDDPTAPSNLYIQE

Target ORF information:

RefSeq Version	XM_011526404.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 1 (MAP4K1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011526404.1

ORF Insert Sequence:

ATGGACGTCG TGGACCCTGA CATTTTCAAT AGAGACCCCC GGGACCACTA TGACCTGCTA 
CAGCGGCTGG GTGGCGGCAC GTATGGGGAA GTCTTTAAGG CTCGAGACAA GGTGTCAGGG 
GACCTGGTGG CACTGAAGAT GGTGAAGATG GAGCCTGATG ATGATGTCTC CACCCTTCAG 
AAGGAAATCC TCATATTGAA AACTTGCCGG CACGCCAACA TCGTGGCCTA CCATGGGAGT 
TATCTCTGGT TGCAGAAACT CTGGATCTGC ATGGAATTCT GTGGGGCTGG TTCTCTCCAG 
GACATCTACC AAGTGACAGG CTCCCTGTCA GAGCTCCAGA TTAGCTATGT CTGCCGGGAA 
GTGCTCCAGG GACTGGCCTA TTTGCACTCA CAGAAGAAGA TACACAGGGA CATCAAGGGA 
GCTAACATCC TCATCAATGA TGCTGGGGAG GTCAGATTGG CTGACTTTGG CATCTCGGCC 
CAGATTGGGG CTACACTGGC CAGACGCCTC TCTTTCATTG GGACACCCTA CTGGATGGCT 
CCGGAAGTGG CAGCTGTGGC CCTGAAGGGA GGATACAATG AGCTGTGTGA CATCTGGTCC 
CTGGGCATCA CGGCCATCGA ACTGGCCGAG CTACAGCCAC CGCTCTTTGA TGTGCACCCT 
CTCAGAGTTC TCTTCCTCAT GACCAAGAGT GGCTACCAGC CTCCCCGACT GAAGGAAAAA 
GGCAAATGGT CGGCTGCCTT CCACAACTTC ATCAAAGTCA CTCTGACTAA GAGTCCCAAG 
AAACGACCCA GCGCCACCAA GATGCTCAGT CATCAACTGG TATCCCAGCC TGGGCTGAAT 
CGAGGCCTGA TCCTGGATCT TCTTGACAAA CTGAAGAATC CCGGGAAAGG ACCCTCCATT 
GGGGACATTG AGGATGAGGA GCCCGAGCTA CCCCCTGCTA TCCCTCGGCG GATCAGATCC 
ACCCACCGCT CCAGCTCTCT GGGGATCCCA GATGCAGACT GCTGTCGGCG GCACATGGAG 
TTCAGGAAGC TCCGAGGAAT GGAGACCAGA CCCCCAGCCA ACACCGCTCG CCTACAGCCT 
CCTCGAGACC TCAGGAGCAG CAGCCCCAGG AAGCAACTGT CAGAGTCGTC TGACGATGAC 
TATGACGACG TGGACATGCT GAACCTTGGC ACCTGGAAAT CTCCCAAAGA CCAGATACTG 
AGCCCCAGAT CCAGACCCAA CAGTCAGCCC AGGCCCCCAT ACTCCCCAGC CCACAGGCCG 
AGTCAGGCTC CATCCAGCCC CACCCCTGCA GAGGACACAC CTCCTCCACT TCCCCCCAAG 
CCCAAGTTCC GTTCTCCATC AGACGAGGGT CCTGGGAGCA TGGGGGATGA TGGGCAGCTG 
AGCCCGGGGG TGCTGGTCCG GTGTGCCAGT GGGCCCCCAC CAAACAGCCC CCGTCCTGGG 
CCTCCCCCAT CCACCAGCAG CCCCCACCTC ACCGCCCATT CAGAACCCTC ACTCTGGAAC 
CCACCCTCCC GGGAGCTTGA CAAGCCCCCA CTTCTGCCCC CCAAGAAGGA AAAGATGAAG 
AGAAAGGGAT GTGCCCTTCT CGTAAAGTTG TTCAATGGCT GCCCCCTCCG GATCCACAGC 
ACGGCCGCCT GGACACATCC CTCCACCAAG GACCAGCACC TGCTCCTGGG GGCAGAGGAA 
GGCATCTTCA TCCTGAACCG GAATGACCAG GAGGCCACGC TGGAAATGCT CTTTCCTAGC 
CGGACTACGT GGGTGTACTC CATCAACAAC GTTCTCATGT CTCTCTCAGG AAAGACCCCC 
CACCTGTATT CTCATAGCAT CCTTGGCCTG CTGGAACGGA AAGAGACCAG AGCAGGAAAC 
CCCATCGCTC ACATTAGCCC CCACCGCCTA CTGGCAAGGA AGAACATGGT TTCCACCAAG 
ATCCAGGACA CCAAAGGCTG CCGGGCGTGC TGTGTGGCGG AGGGTGCGAG CTCTGGGGGC 
CCGTTCCTGT GCGGTGCATT GGAGACGTCC GTTGTCCTGC TTCAGTGGTA CCAGCCCATG 
AACAAATTCC TGCTTGTCCG GCAGGTGCTG TTCCCACTGC CGACGCCTCT GTCCGTGTTC 
GCGCTGCTGA CCGGGCCAGG CTCTGAGCTG CCCGCTGTGT GCATCGGCGT GAGCCCCGGG 
CGGCCGGGGA AGTCGGTGCT CTTCCACACG GTGCGCTTTG GCGCGCTCTC TTGCTGGCTG 
GGCGAGATGA GCACCGAGCA CAGGGGACCC GTGCAGGTGA CCCAGGTAGA GGAAGATATG 
GTGATGGTGT TGATGGATGG CTCTGTGAAG CTGGTGACCC CGGAGGGGTC CCCAGTCCGG 
GGACTTCGCA CACCTGAGAT CCCCATGACC GAAGCGGTGG AGGCCGTGGC TATGGTTGGA 
GGTCAGCTTC AGGCCTTCTG GAAGCATGGA GTGCAGGTGT GGGCTCTAGG CTCGGATCAG 
CTGCTACAGG AGCTGAGAGA CCCTACCCTC ACTTTCCGTC TGCTTGGCTC CCCCAGGCCT 
GTAGTGGTGG AGACACGCCC AGTGGATGAT CCTACTGCTC CCAGCAACCT CTACATCCAG 
GAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP4K1 ( XM_017026231.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016881720.1 Homo sapiens mitogen-activated protein kinase kinase kinase kinase 1 (MAP4K1), transcript variant X3, mRNA.

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CloneID	OHu88172
Clone ID Related Accession (Same CDS sequence)	XM_017026231.1
Accession Version	XM_017026231.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2583bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	mitogen-activated protein kinase kinase kinase kinase 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTGGA GGGACTCTGG CAAATGGAGC CTGCCTGACT GCCAACTTCC CCTTTGCAAG 
GCTCGAGACA AGGTGTCAGG GGACCTGGTG GCACTGAAGA TGGTGAAGAT GGAGCCTGAT 
GATGATGTCT CCACCCTTCA GAAGGAAATC CTCATATTGA AAACTTGCCG GCACGCCAAC 
ATCGTGGCCT ACCATGGGAG TTATCTCTGG TTGCAGAAAC TCTGGATCTG CATGGAATTC 
TGTGGGGCTG GTTCTCTCCA GGACATCTAC CAAGTGACAG GCTCCCTGTC AGAGCTCCAG 
ATTAGCTATG TCTGCCGGGA AGTGCTCCAG GGACTGGCCT ATTTGCACTC ACAGAAGAAG 
ATACACAGGG ACATCAAGGG AGCTAACATC CTCATCAATG ATGCTGGGGA GGTCAGATTG 
GCTGACTTTG GCATCTCGGC CCAGATTGGG GCTACACTGG CCAGACGCCT CTCTTTCATT 
GGGACACCCT ACTGGATGGC TCCGGAAGTG GCAGCTGTGG CCCTGAAGGG AGGATACAAT 
GAGCTGTGTG ACATCTGGTC CCTGGGCATC ACGGCCATCG AACTGGCCGA GCTACAGCCA 
CCGCTCTTTG ATGTGCACCC TCTCAGAGTT CTCTTCCTCA TGACCAAGAG TGGCTACCAG 
CCTCCCCGAC TGAAGGAAAA AGGCAAATGG TCGGCTGCCT TCCACAACTT CATCAAAGTC 
ACTCTGACTA AGAGTCCCAA GAAACGACCC AGCGCCACCA AGATGCTCAG TCATCAACTG 
GTATCCCAGC CTGGGCTGAA TCGAGGCCTG ATCCTGGATC TTCTTGACAA ACTGAAGAAT 
CCCGGGAAAG GACCCTCCAT TGGGGACATT GAGGATGAGG AGCCCGAGCT ACCCCCTGCT 
ATCCCTCGGC GGATCAGATC CACCCACCGC TCCAGCTCTC TGGGGATCCC AGATGCAGAC 
TGCTGTCGGC GGCACATGGA GTTCAGGAAG CTCCGAGGAA TGGAGACCAG ACCCCCAGCC 
AACACCGCTC GCCTACAGCC TCCTCGAGAC CTCAGGAGCA GCAGCCCCAG GAAGCAACTG 
TCAGAGTCGT CTGACGATGA CTATGACGAC GTGGACATGC TGAACCTTGG CACCTGGAAA 
TCTCCCAAAG ACCAGATACT GAGCCCCAGA TCCAGACCCA ACAGTCAGCC CAGGCCCCCA 
TACTCCCCAG CCCACAGGCC GAGTCAGGCT CCATCCAGCC CCACCCCTGC AGAGGACACA 
CCTCCTCCAC TTCCCCCCAA GCCCAAGTTC CGTTCTCCAT CAGACGAGGG TCCTGGGAGC 
ATGGGGGATG ATGGGCAGCT GAGCCCGGGG GTGCTGGTCC GGTGTGCCAG TGGGCCCCCA 
CCAAACAGCC CCCGTCCTGG GCCTCCCCCA TCCACCAGCA GCCCCCACCT CACCGCCCAT 
TCAGAACCCT CACTCTGGAA CCCACCCTCC CGGGAGCTTG ACAAGCCCCC ACTTCTGCCC 
CCCAAGAAGG AAAAGATGAA GAGAAAGGGA TGTGCCCTTC TCGTAAAGTT GTTCAATGGC 
TGCCCCCTCC GGATCCACAG CACGGCCGCC TGGACACATC CCTCCACCAA GGACCAGCAC 
CTGCTCCTGG GGGCAGAGGA AGGCATCTTC ATCCTGAACC GGAATGACCA GGAGGCCACG 
CTGGAAATGC TCTTTCCTAG CCGGACTACG TGGGTGTACT CCATCAACAA CGTTCTCATG 
TCTCTCTCAG GAAAGACCCC CCACCTGTAT TCTCATAGCA TCCTTGGCCT GCTGGAACGG 
AAAGAGACCA GAGCAGGAAA CCCCATCGCT CACATTAGCC CCCACCGCCT ACTGGCAAGG 
AAGAACATGG TTTCCACCAA GATCCAGGAC ACCAAAGGCT GCCGGGCGTG CTGTGTGGCG 
GAGGGTGCGA GCTCTGGGGG CCCGTTCCTG TGCGGTGCAT TGGAGACGTC CGTTGTCCTG 
CTTCAGTGGT ACCAGCCCAT GAACAAATTC CTGCTTGTCC GGCAGGTGCT GTTCCCACTG 
CCGACGCCTC TGTCCGTGTT CGCGCTGCTG ACCGGGCCAG GCTCTGAGCT GCCCGCTGTG 
TGCATCGGCG TGAGCCCCGG GCGGCCGGGG AAGTCGGTGC TCTTCCACAC GGTGCGCTTT 
GGCGCGCTCT CTTGCTGGCT GGGCGAGATG AGCACCGAGC ACAGGGGACC CGTGCAGGTG 
ACCCAGGTAG AGGAAGATAT GGTGATGGTG TTGATGGATG GCTCTGTGAA GCTGGTGACC 
CCGGAGGGGT CCCCAGTCCG GGGACTTCGC ACACCTGAGA TCCCCATGAC CGAAGCGGTG 
GAGGCCGTGG CTATGGTTGG AGGTCAGCTT CAGGCCTTCT GGAAGCATGG AGTGCAGGTG 
TGGGCTCTAG GCTCGGATCA GCTGCTACAG GAGCTGAGAG ACCCTACCCT CACTTTCCGT 
CTGCTTGGCT CCCCCAGGCT GGAGTGCAGT GGCACGATCT CGCCTCACTG CAACCTCCTC 
CTCCCAGGTT CAAGCAATTC TCCTGCCTCA GCCTCCCGAG TAGCTGGGAT TACAGGCCTG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016881720.1
CDS	86..2668
Translation	MLWRDSGKWSLPDCQLPLCKARDKVSGDLVALKMVKMEPDDDVSTLQKEILILKTCRHANIVAYHGSYLWLQKLWICMEFCGAGSLQDIYQVTGSLSELQISYVCREVLQGLAYLHSQKKIHRDIKGANILINDAGEVRLADFGISAQIGATLARRLSFIGTPYWMAPEVAAVALKGGYNELCDIWSLGITAIELAELQPPLFDVHPLRVLFLMTKSGYQPPRLKEKGKWSAAFHNFIKVTLTKSPKKRPSATKMLSHQLVSQPGLNRGLILDLLDKLKNPGKGPSIGDIEDEEPELPPAIPRRIRSTHRSSSLGIPDADCCRRHMEFRKLRGMETRPPANTARLQPPRDLRSSSPRKQLSESSDDDYDDVDMLNLGTWKSPKDQILSPRSRPNSQPRPPYSPAHRPSQAPSSPTPAEDTPPPLPPKPKFRSPSDEGPGSMGDDGQLSPGVLVRCASGPPPNSPRPGPPPSTSSPHLTAHSEPSLWNPPSRELDKPPLLPPKKEKMKRKGCALLVKLFNGCPLRIHSTAAWTHPSTKDQHLLLGAEEGIFILNRNDQEATLEMLFPSRTTWVYSINNVLMSLSGKTPHLYSHSILGLLERKETRAGNPIAHISPHRLLARKNMVSTKIQDTKGCRACCVAEGASSGGPFLCGALETSVVLLQWYQPMNKFLLVRQVLFPLPTPLSVFALLTGPGSELPAVCIGVSPGRPGKSVLFHTVRFGALSCWLGEMSTEHRGPVQVTQVEEDMVMVLMDGSVKLVTPEGSPVRGLRTPEIPMTEAVEAVAMVGGQLQAFWKHGVQVWALGSDQLLQELRDPTLTFRLLGSPRLECSGTISPHCNLLLPGSSNSPASASRVAGITGL

Target ORF information:

RefSeq Version	XM_017026231.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 1 (MAP4K1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017026231.1

ORF Insert Sequence:

ATGCTGTGGA GGGACTCTGG CAAATGGAGC CTGCCTGACT GCCAACTTCC CCTTTGCAAG 
GCTCGAGACA AGGTGTCAGG GGACCTGGTG GCACTGAAGA TGGTGAAGAT GGAGCCTGAT 
GATGATGTCT CCACCCTTCA GAAGGAAATC CTCATATTGA AAACTTGCCG GCACGCCAAC 
ATCGTGGCCT ACCATGGGAG TTATCTCTGG TTGCAGAAAC TCTGGATCTG CATGGAATTC 
TGTGGGGCTG GTTCTCTCCA GGACATCTAC CAAGTGACAG GCTCCCTGTC AGAGCTCCAG 
ATTAGCTATG TCTGCCGGGA AGTGCTCCAG GGACTGGCCT ATTTGCACTC ACAGAAGAAG 
ATACACAGGG ACATCAAGGG AGCTAACATC CTCATCAATG ATGCTGGGGA GGTCAGATTG 
GCTGACTTTG GCATCTCGGC CCAGATTGGG GCTACACTGG CCAGACGCCT CTCTTTCATT 
GGGACACCCT ACTGGATGGC TCCGGAAGTG GCAGCTGTGG CCCTGAAGGG AGGATACAAT 
GAGCTGTGTG ACATCTGGTC CCTGGGCATC ACGGCCATCG AACTGGCCGA GCTACAGCCA 
CCGCTCTTTG ATGTGCACCC TCTCAGAGTT CTCTTCCTCA TGACCAAGAG TGGCTACCAG 
CCTCCCCGAC TGAAGGAAAA AGGCAAATGG TCGGCTGCCT TCCACAACTT CATCAAAGTC 
ACTCTGACTA AGAGTCCCAA GAAACGACCC AGCGCCACCA AGATGCTCAG TCATCAACTG 
GTATCCCAGC CTGGGCTGAA TCGAGGCCTG ATCCTGGATC TTCTTGACAA ACTGAAGAAT 
CCCGGGAAAG GACCCTCCAT TGGGGACATT GAGGATGAGG AGCCCGAGCT ACCCCCTGCT 
ATCCCTCGGC GGATCAGATC CACCCACCGC TCCAGCTCTC TGGGGATCCC AGATGCAGAC 
TGCTGTCGGC GGCACATGGA GTTCAGGAAG CTCCGAGGAA TGGAGACCAG ACCCCCAGCC 
AACACCGCTC GCCTACAGCC TCCTCGAGAC CTCAGGAGCA GCAGCCCCAG GAAGCAACTG 
TCAGAGTCGT CTGACGATGA CTATGACGAC GTGGACATGC TGAACCTTGG CACCTGGAAA 
TCTCCCAAAG ACCAGATACT GAGCCCCAGA TCCAGACCCA ACAGTCAGCC CAGGCCCCCA 
TACTCCCCAG CCCACAGGCC GAGTCAGGCT CCATCCAGCC CCACCCCTGC AGAGGACACA 
CCTCCTCCAC TTCCCCCCAA GCCCAAGTTC CGTTCTCCAT CAGACGAGGG TCCTGGGAGC 
ATGGGGGATG ATGGGCAGCT GAGCCCGGGG GTGCTGGTCC GGTGTGCCAG TGGGCCCCCA 
CCAAACAGCC CCCGTCCTGG GCCTCCCCCA TCCACCAGCA GCCCCCACCT CACCGCCCAT 
TCAGAACCCT CACTCTGGAA CCCACCCTCC CGGGAGCTTG ACAAGCCCCC ACTTCTGCCC 
CCCAAGAAGG AAAAGATGAA GAGAAAGGGA TGTGCCCTTC TCGTAAAGTT GTTCAATGGC 
TGCCCCCTCC GGATCCACAG CACGGCCGCC TGGACACATC CCTCCACCAA GGACCAGCAC 
CTGCTCCTGG GGGCAGAGGA AGGCATCTTC ATCCTGAACC GGAATGACCA GGAGGCCACG 
CTGGAAATGC TCTTTCCTAG CCGGACTACG TGGGTGTACT CCATCAACAA CGTTCTCATG 
TCTCTCTCAG GAAAGACCCC CCACCTGTAT TCTCATAGCA TCCTTGGCCT GCTGGAACGG 
AAAGAGACCA GAGCAGGAAA CCCCATCGCT CACATTAGCC CCCACCGCCT ACTGGCAAGG 
AAGAACATGG TTTCCACCAA GATCCAGGAC ACCAAAGGCT GCCGGGCGTG CTGTGTGGCG 
GAGGGTGCGA GCTCTGGGGG CCCGTTCCTG TGCGGTGCAT TGGAGACGTC CGTTGTCCTG 
CTTCAGTGGT ACCAGCCCAT GAACAAATTC CTGCTTGTCC GGCAGGTGCT GTTCCCACTG 
CCGACGCCTC TGTCCGTGTT CGCGCTGCTG ACCGGGCCAG GCTCTGAGCT GCCCGCTGTG 
TGCATCGGCG TGAGCCCCGG GCGGCCGGGG AAGTCGGTGC TCTTCCACAC GGTGCGCTTT 
GGCGCGCTCT CTTGCTGGCT GGGCGAGATG AGCACCGAGC ACAGGGGACC CGTGCAGGTG 
ACCCAGGTAG AGGAAGATAT GGTGATGGTG TTGATGGATG GCTCTGTGAA GCTGGTGACC 
CCGGAGGGGT CCCCAGTCCG GGGACTTCGC ACACCTGAGA TCCCCATGAC CGAAGCGGTG 
GAGGCCGTGG CTATGGTTGG AGGTCAGCTT CAGGCCTTCT GGAAGCATGG AGTGCAGGTG 
TGGGCTCTAG GCTCGGATCA GCTGCTACAG GAGCTGAGAG ACCCTACCCT CACTTTCCGT 
CTGCTTGGCT CCCCCAGGCT GGAGTGCAGT GGCACGATCT CGCCTCACTG CAACCTCCTC 
CTCCCAGGTT CAAGCAATTC TCCTGCCTCA GCCTCCCGAG TAGCTGGGAT TACAGGCCTG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Interactions between hematopoietic progenitor kinase 1 and its adaptor proteins (Review).
Molecular medicine reports16(5)6472-6482(2017 Nov)
Zhang Q,Ding S,Zhang H

HPK1 positive expression associated with longer overall survival in patients with estrogen receptor-positive invasive ductal carcinoma?not otherwise specified.
Molecular medicine reports16(4)4634-4642(2017 Oct)
Wang J,Song L,Yang S,Zhang W,Lu P,Li S,Li H,Wang L

The CUL7/F-box and WD repeat domain containing 8 (CUL7/Fbxw8) ubiquitin ligase promotes degradation of hematopoietic progenitor kinase 1.
The Journal of biological chemistry289(7)4009-17(2014 Feb)
Wang H,Chen Y,Lin P,Li L,Zhou G,Liu G,Logsdon C,Jin J,Abbruzzese JL,Tan TH,Wang H

Hematopoietic progenitor kinase 1 (HPK1) is required for LFA-1-mediated neutrophil recruitment during the acute inflammatory response.
Blood121(20)4184-94(2013 May)
Jakob SM,Pick R,Brechtefeld D,Nussbaum C,Kiefer F,Sperandio M,Walzog B

Pdcd4 knockdown up-regulates MAP4K1 expression and activation of AP-1 dependent transcription through c-Myc.
Biochimica et biophysica acta1823(10)1807-14(2012 Oct)
Wang Q,Zhang Y,Yang HS

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MAP4K1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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