CAPN10 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	CAPN10
Entrez Gene ID	11132
Full Name	calpain 10
Synonyms	CANP10,NIDDM1
General protein information	Preferred Names calpain 10 Names calpain-10 calcium-activated neutral proteinase 10 calpain-like protease CAPN10
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 2 Map Location: 2q37.3
Summary	Calpains represent a ubiquitous, well-conserved family of calcium-dependent cysteine proteases. The calpain proteins are heterodimers consisting of an invariant small subunit and variable large subunits. The large catalytic subunit has four domains: domain I, the N-terminal regulatory domain that is processed upon calpain activation; domain II, the protease domain; domain III, a linker domain of unknown function; and domain IV, the calmodulin-like calcium-binding domain. This gene encodes a large subunit. It is an atypical calpain in that it lacks the calmodulin-like calcium-binding domain and instead has a divergent C-terminal domain. It is similar in organization to calpains 5 and 6. This gene is associated with type 2 or non-insulin-dependent diabetes mellitus (NIDDM), and is located within the NIDDM1 region. Multiple alternative transcript variants have been described for this gene. [provided by RefSeq, Sep 2010].
Disorder MIM:	605286
Disorder Html:	{Diabetes mellitus, noninsulin-dependent 1}, 601283 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_023083.4	NP_075571.2	calpain-10 isoform a
NM_023085.3	NP_075573.2	calpain-10 isoform c
NM_023085.4	NP_075573.3	calpain-10 isoform c
NM_023083.3	NP_075571.1	calpain-10 isoform a

Pathways from BioSystems

Homologies

	CAPN10	XP_001107045.2
Rattus norvegicus (Norway rat)	Capn10	NP_113861.1
Danio rerio (zebrafish)	capn10	XP_698873.4
Homo sapiens (human)	CAPN10	NP_075571.1
Xenopus tropicalis (tropical clawed frog)	capn10	XP_004914447.1
Pan troglodytes (chimpanzee)	CAPN10	XP_003309611.1
Bos taurus (cattle)	LOC789674	XP_002686664.1
Canis lupus familiaris (dog)	CAPN10	XP_848308.1
Mus musculus (house mouse)	Capn10	NP_035926.2
Gallus gallus (chicken)	CAPN10	XP_004943444.1

Gene Ontology
Bibliography

Related articles in PubMed

Correlation between Calpain-10 single-nucleotide polymorphisms and obstructive sleep apnea/hypopnoea syndrome with ischemic stroke in a Chinese population: A population-based study.
Zhang W, Zhao ZR, Dai CF, Zhang R, Chen J, Tian HJ, Wang YL, Sun JH, Lian QF
Medicine96(16)e6570(2017 Apr)

Single-nucleotide polymorphism of INS, INSR, IRS1, IRS2, PPAR-G and CAPN10 genes in the pathogenesis of polycystic ovary syndrome.
Thangavelu M, Godla UR, Paul Solomon FD, Maddaly R
Journal of genetics96(1)87-96(2017 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

TCF7L2 rs7903146 and 112/112 haplotype of CAPN10 might be associated with gestational diabetes risks. [meta-analysis]
Title: TCF7L2, CAPN10 polymorphisms are associated with gestational diabetes mellitus (GDM) risks: a meta-analysis.

These findings indicate that the Calpain-10 SNP 43 may be related to obstructive sleep apnea/hypopnea syndrome with ischemic stroke, with SNP 43 GG genotype as a risk factor for obstructive sleep apnea/hypopnea with ischemic stroke
Title: Correlation between Calpain-10 single-nucleotide polymorphisms and obstructive sleep apnea/hypopnoea syndrome with ischemic stroke in a Chinese population: A population-based study.

The study results were suggestive of a positive association between Gly972Arg of IRS1 and PCOS in the south Indian population, while INS, IRS2, PPAR-G and CAPN10 failed to show any association with PCOS in our studied population.
Title: Single-nucleotide polymorphism of INS, INSR, IRS1, IRS2, PPAR-G and CAPN10 genes in the pathogenesis of polycystic ovary syndrome.

There were significant differences between the type 2 diabetes mellitus patients and controls in the risk allele distributions of rs3792267 (CAPN10) (P = 0.002), rs1501299 (APM1) (P = 0.017), and rs3760776 (FUT6) (P = 0.031).
Title: The Uyghur population and genetic susceptibility to type 2 diabetes: potential role for variants in CAPN10, APM1 and FUT6 genes.

association between SNP 63 of CAPN10 and gestational diabetes mellitus is only significant in the heterozygous model
Title: Meta-analysis of the association between four CAPN10 gene variants and gestational diabetes mellitus.

The following CAPN10 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CAPN10 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu18163	NM_023083.3 Latest version!	Homo sapiens calpain 10 (CAPN10), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu107793	NM_023083.4 Latest version!	Homo sapiens calpain 10 (CAPN10), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu108241	NM_023085.4 Latest version!	Homo sapiens calpain 10 (CAPN10), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OHu15864	NM_023085.3 Latest version!	Homo sapiens calpain 10 (CAPN10), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$300.30 $429.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CAPN10 ( NM_023083.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_075571.1 Homo sapiens calpain 10 (CAPN10), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu18163
Clone ID Related Accession (Same CDS sequence)	NM_023083.3
Accession Version	NM_023083.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2019bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-27
Organism	Homo sapiens(human)
Product	calpain-10 isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL558905.3, AF089088.1, AK074807.1, AU310002.1 and BQ052841.1. This sequence is a reference standard in the RefSeqGene project. On Sep 3, 2010 this sequence version replaced NM_023083.2. Transcript Variant: This variant (1, also known as CAPN10a) represents the longer transcript and encodes the longer isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK074807.1, AF089088.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCGGGCGG GCCGGGGCGC GACGCCGGCG AGGGAGCTGT TCCGGGACGC CGCCTTCCCC 
GCCGCGGACT CCTCGCTCTT CTGCGACTTG TCTACGCCGC TGGCCCAGTT CCGCGAGGAC 
ATCACGTGGA GGCGGCCCCA GGAGATTTGT GCCACACCCC GGCTGTTTCC AGATGACCCA 
CGGGAAGGGC AGGTGAAGCA GGGGCTGCTG GGGGATTGCT GGTTCCTGTG TGCCTGCGCC 
GCGCTGCAGA AGAGCAGGCA CCTCCTGGAC CAGGTCATTC CTCCGGGACA GCCGAGCTGG 
GCCGACCAGG AGTACCGGGG CTCCTTCACC TGTCGCATTT GGCAGTTTGG ACGCTGGGTG 
GAGGTGACCA CAGATGACCG CCTGCCGTGC CTTGCAGGGA GACTCTGTTT CTCCCGCTGC 
CAGAGGGAGG ATGTGTTCTG GCTCCCCTTA CTGGAAAAGG TCTACGCCAA GGTCCATGGG 
TCCTACGAGC ACCTGTGGGC CGGGCAGGTG GCGGATGCCC TGGTGGACCT GACCGGCGGC 
CTGGCAGAAA GATGGAACCT GAAGGGCGTA GCAGGAAGCG GAGGCCAGCA GGACAGGCCA 
GGCCGCTGGG AGCACAGGAC TTGTCGGCAG CTGCTCCACC TGAAGGACCA GTGTCTGATC 
AGCTGCTGCG TGCTCAGCCC CAGAGCAGGT GCCCGGGAGC TGGGGGAGTT CCATGCCTTC 
ATTGTCTCGG ACCTGCGGGA GCTCCAGGGT CAGGCGGGCC AGTGCATCCT GCTGCTGCGG 
ATCCAGAACC CCTGGGGCCG GCGGTGCTGG CAGGGGCTCT GGAGAGAGGG GGGTGAAGGG 
TGGAGCCAGG TAGATGCAGC GGTAGCATCT GAGCTCCTGT CCCAGCTCCA GGAAGGGGAG 
TTCTGGGTGG AGGAGGAGGA GTTCCTCAGG GAGTTTGACG AGCTCACCGT TGGCTACCCG 
GTCACGGAGG CCGGCCACCT GCAGAGCCTC TACACAGAGA GGCTGCTCTG CCATACGCGG 
GCGCTGCCTG GGGCCTGGGT CAAGGGCCAG TCAGCAGGAG GCTGCCGGAA CAACAGCGGC 
TTTCCCAGCA ACCCCAAATT CTGGCTGCGG GTCTCAGAAC CGAGTGAGGT GTACATTGCC 
GTCCTGCAGA GATCCAGGCT GCACGCGGCG GACTGGGCAG GCCGGGCCCG GGCACTGGTG 
GGTGACAGTC ATACTTCGTG GAGCCCAGCG AGCATCCCGG GCAAGCACTA CCAGGCTGTG 
GGTCTGCACC TCTGGAAGGT AGAGAAGCGG CGGGTCAATC TGCCTAGGGT CCTGTCCATG 
CCCCCCGTGG CTGGCACCGC GTGCCATGCA TACGACCGGG AGGTCCACCT GCGTTGTGAG 
CTCTCACCGG GCTACTACCT GGCTGTCCCC AGCACCTTCC TGAAGGACGC GCCAGGGGAG 
TTCCTGCTCC GAGTCTTCTC TACCGGGCGA GTCTCCCTTA GCGCCATCAG GGCAGTGGCC 
AAGAACACCA CCCCCGGGGC AGCCCTGCCT GCGGGGGAGT GGGGGACCGT GCAGCTACGG 
GGTTCTTGGA GAGTCGGCCA GACGGCGGGG GGCAGCAGGA ACTTTGCCTC ATACCCCACC 
AACCCCTGCT TCCCCTTCTC GGTCCCCGAG GGCCCTGGCC CCCGCTGCGT CCGCATCACT 
CTGCATCAGC ACTGCCGGCC CAGTGACACC GAGTTCCACC CCATCGGCTT CCATATCTTC 
CAGGTCCCAG AGGGTGGAAG GAGCCAGGAC GCACCCCCAC TGCTGCTGCA GGAGCCGCTG 
CTGAGCTGCG TGCCACATCG CTACGCCCAG GAGGTGAGCC GGCTCTGCCT CCTGCCTGCG 
GGCACCTACA AGGTTGTGCC CTCCACCTAC CTGCCGGACA CAGAGGGGGC CTTCACAGTG 
ACCATCGCAA CCAGGATTGA CAGGCCATCC ATTCACAGCC AGGAGATGCT GGGCCAGTTC 
CTCCAAGAGG TCTCCGTCAT GGCAGTGATG AAAACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_075571.1
CDS	197..2215
Translation	MRAGRGATPARELFRDAAFPAADSSLFCDLSTPLAQFREDITWRRPQEICATPRLFPDDPREGQVKQGLLGDCWFLCACAALQKSRHLLDQVIPPGQPSWADQEYRGSFTCRIWQFGRWVEVTTDDRLPCLAGRLCFSRCQREDVFWLPLLEKVYAKVHGSYEHLWAGQVADALVDLTGGLAERWNLKGVAGSGGQQDRPGRWEHRTCRQLLHLKDQCLISCCVLSPRAGARELGEFHAFIVSDLRELQGQAGQCILLLRIQNPWGRRCWQGLWREGGEGWSQVDAAVASELLSQLQEGEFWVEEEEFLREFDELTVGYPVTEAGHLQSLYTERLLCHTRALPGAWVKGQSAGGCRNNSGFPSNPKFWLRVSEPSEVYIAVLQRSRLHAADWAGRARALVGDSHTSWSPASIPGKHYQAVGLHLWKVEKRRVNLPRVLSMPPVAGTACHAYDREVHLRCELSPGYYLAVPSTFLKDAPGEFLLRVFSTGRVSLSAIRAVAKNTTPGAALPAGEWGTVQLRGSWRVGQTAGGSRNFASYPTNPCFPFSVPEGPGPRCVRITLHQHCRPSDTEFHPIGFHIFQVPEGGRSQDAPPLLLQEPLLSCVPHRYAQEVSRLCLLPAGTYKVVPSTYLPDTEGAFTVTIATRIDRPSIHSQEMLGQFLQEVSVMAVMKT

Target ORF information:

RefSeq Version	NM_023083.3
Organism	Homo sapiens(human)
Definition	Homo sapiens calpain 10 (CAPN10), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_023083.3

ORF Insert Sequence:

ATGCGGGCGG GCCGGGGCGC GACGCCGGCG AGGGAGCTGT TCCGGGACGC CGCCTTCCCC 
GCCGCGGACT CCTCGCTCTT CTGCGACTTG TCTACGCCGC TGGCCCAGTT CCGCGAGGAC 
ATCACGTGGA GGCGGCCCCA GGAGATTTGT GCCACACCCC GGCTGTTTCC AGATGACCCA 
CGGGAAGGGC AGGTGAAGCA GGGGCTGCTG GGGGATTGCT GGTTCCTGTG TGCCTGCGCC 
GCGCTGCAGA AGAGCAGGCA CCTCCTGGAC CAGGTCATTC CTCCGGGACA GCCGAGCTGG 
GCCGACCAGG AGTACCGGGG CTCCTTCACC TGTCGCATTT GGCAGTTTGG ACGCTGGGTG 
GAGGTGACCA CAGATGACCG CCTGCCGTGC CTTGCAGGGA GACTCTGTTT CTCCCGCTGC 
CAGAGGGAGG ATGTGTTCTG GCTCCCCTTA CTGGAAAAGG TCTACGCCAA GGTCCATGGG 
TCCTACGAGC ACCTGTGGGC CGGGCAGGTG GCGGATGCCC TGGTGGACCT GACCGGCGGC 
CTGGCAGAAA GATGGAACCT GAAGGGCGTA GCAGGAAGCG GAGGCCAGCA GGACAGGCCA 
GGCCGCTGGG AGCACAGGAC TTGTCGGCAG CTGCTCCACC TGAAGGACCA GTGTCTGATC 
AGCTGCTGCG TGCTCAGCCC CAGAGCAGGT GCCCGGGAGC TGGGGGAGTT CCATGCCTTC 
ATTGTCTCGG ACCTGCGGGA GCTCCAGGGT CAGGCGGGCC AGTGCATCCT GCTGCTGCGG 
ATCCAGAACC CCTGGGGCCG GCGGTGCTGG CAGGGGCTCT GGAGAGAGGG GGGTGAAGGG 
TGGAGCCAGG TAGATGCAGC GGTAGCATCT GAGCTCCTGT CCCAGCTCCA GGAAGGGGAG 
TTCTGGGTGG AGGAGGAGGA GTTCCTCAGG GAGTTTGACG AGCTCACCGT TGGCTACCCG 
GTCACGGAGG CCGGCCACCT GCAGAGCCTC TACACAGAGA GGCTGCTCTG CCATACGCGG 
GCGCTGCCTG GGGCCTGGGT CAAGGGCCAG TCAGCAGGAG GCTGCCGGAA CAACAGCGGC 
TTTCCCAGCA ACCCCAAATT CTGGCTGCGG GTCTCAGAAC CGAGTGAGGT GTACATTGCC 
GTCCTGCAGA GATCCAGGCT GCACGCGGCG GACTGGGCAG GCCGGGCCCG GGCACTGGTG 
GGTGACAGTC ATACTTCGTG GAGCCCAGCG AGCATCCCGG GCAAGCACTA CCAGGCTGTG 
GGTCTGCACC TCTGGAAGGT AGAGAAGCGG CGGGTCAATC TGCCTAGGGT CCTGTCCATG 
CCCCCCGTGG CTGGCACCGC GTGCCATGCA TACGACCGGG AGGTCCACCT GCGTTGTGAG 
CTCTCACCGG GCTACTACCT GGCTGTCCCC AGCACCTTCC TGAAGGACGC GCCAGGGGAG 
TTCCTGCTCC GAGTCTTCTC TACCGGGCGA GTCTCCCTTA GCGCCATCAG GGCAGTGGCC 
AAGAACACCA CCCCCGGGGC AGCCCTGCCT GCGGGGGAGT GGGGGACCGT GCAGCTACGG 
GGTTCTTGGA GAGTCGGCCA GACGGCGGGG GGCAGCAGGA ACTTTGCCTC ATACCCCACC 
AACCCCTGCT TCCCCTTCTC GGTCCCCGAG GGCCCTGGCC CCCGCTGCGT CCGCATCACT 
CTGCATCAGC ACTGCCGGCC CAGTGACACC GAGTTCCACC CCATCGGCTT CCATATCTTC 
CAGGTCCCAG AGGGTGGAAG GAGCCAGGAC GCACCCCCAC TGCTGCTGCA GGAGCCGCTG 
CTGAGCTGCG TGCCACATCG CTACGCCCAG GAGGTGAGCC GGCTCTGCCT CCTGCCTGCG 
GGCACCTACA AGGTTGTGCC CTCCACCTAC CTGCCGGACA CAGAGGGGGC CTTCACAGTG 
ACCATCGCAA CCAGGATTGA CAGGCCATCC ATTCACAGCC AGGAGATGCT GGGCCAGTTC 
CTCCAAGAGG TCTCCGTCAT GGCAGTGATG AAAACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAPN10 ( NM_023083.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_075571.2 Homo sapiens calpain 10 (CAPN10), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu107793
Clone ID Related Accession (Same CDS sequence)	NM_023083.4
Accession Version	NM_023083.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2019bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-29
Organism	Homo sapiens(human)
Product	calpain-10 isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC124862.4. On Nov 23, 2018 this sequence version replaced NM_023083.3. Transcript Variant: This variant (1, also known as CAPN10a) represents the longer transcript and encodes the longer isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF089088.1, AK074807.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000391984.7/ ENSP00000375844.2 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCGGGCGG GCCGGGGCGC GACGCCGGCG AGGGAGCTGT TCCGGGACGC CGCCTTCCCC 
GCCGCGGACT CCTCGCTCTT CTGCGACTTG TCTACGCCGC TGGCCCAGTT CCGCGAGGAC 
ATCACGTGGA GGCGGCCCCA GGAGATTTGT GCCACACCCC GGCTGTTTCC AGATGACCCA 
CGGGAAGGGC AGGTGAAGCA GGGGCTGCTG GGGGATTGCT GGTTCCTGTG TGCCTGCGCC 
GCGCTGCAGA AGAGCAGGCA CCTCCTGGAC CAGGTCATTC CTCCGGGACA GCCGAGCTGG 
GCCGACCAGG AGTACCGGGG CTCCTTCACC TGTCGCATTT GGCAGTTTGG ACGCTGGGTG 
GAGGTGACCA CAGATGACCG CCTGCCGTGC CTTGCAGGGA GACTCTGTTT CTCCCGCTGC 
CAGAGGGAGG ATGTGTTCTG GCTCCCCTTA CTGGAAAAGG TCTACGCCAA GGTCCATGGG 
TCCTACGAGC ACCTGTGGGC CGGGCAGGTG GCGGATGCCC TGGTGGACCT GACCGGCGGC 
CTGGCAGAAA GATGGAACCT GAAGGGCGTA GCAGGAAGCG GAGGCCAGCA GGACAGGCCA 
GGCCGCTGGG AGCACAGGAC TTGTCGGCAG CTGCTCCACC TGAAGGACCA GTGTCTGATC 
AGCTGCTGCG TGCTCAGCCC CAGAGCAGGT GCCCGGGAGC TGGGGGAGTT CCATGCCTTC 
ATTGTCTCGG ACCTGCGGGA GCTCCAGGGT CAGGCGGGCC AGTGCATCCT GCTGCTGCGG 
ATCCAGAACC CCTGGGGCCG GCGGTGCTGG CAGGGGCTCT GGAGAGAGGG GGGTGAAGGG 
TGGAGCCAGG TAGATGCAGC GGTAGCATCT GAGCTCCTGT CCCAGCTCCA GGAAGGGGAG 
TTCTGGGTGG AGGAGGAGGA GTTCCTCAGG GAGTTTGACG AGCTCACCGT TGGCTACCCG 
GTCACGGAGG CCGGCCACCT GCAGAGCCTC TACACAGAGA GGCTGCTCTG CCATACGCGG 
GCGCTGCCTG GGGCCTGGGT CAAGGGCCAG TCAGCAGGAG GCTGCCGGAA CAACAGCGGC 
TTTCCCAGCA ACCCCAAATT CTGGCTGCGG GTCTCAGAAC CGAGTGAGGT GTACATTGCC 
GTCCTGCAGA GATCCAGGCT GCACGCGGCG GACTGGGCAG GCCGGGCCCG GGCACTGGTG 
GGTGACAGTC ATACTTCGTG GAGCCCAGCG AGCATCCCGG GCAAGCACTA CCAGGCTGTG 
GGTCTGCACC TCTGGAAGGT AGAGAAGCGG CGGGTCAATC TGCCTAGGGT CCTGTCCATG 
CCCCCCGTGG CTGGCACCGC GTGCCATGCA TACGACCGGG AGGTCCACCT GCGTTGTGAG 
CTCTCACCGG GCTACTACCT GGCTGTCCCC AGCACCTTCC TGAAGGACGC GCCAGGGGAG 
TTCCTGCTCC GAGTCTTCTC TACCGGGCGA GTCTCCCTTA GCGCCATCAG GGCAGTGGCC 
AAGAACACCA CCCCCGGGGC AGCCCTGCCT GCGGGGGAGT GGGGGACCGT GCAGCTACGG 
GGTTCTTGGA GAGTCGGCCA GACGGCGGGG GGCAGCAGGA ACTTTGCCTC ATACCCCACC 
AACCCCTGCT TCCCCTTCTC GGTCCCCGAG GGCCCTGGCC CCCGCTGCGT CCGCATCACT 
CTGCATCAGC ACTGCCGGCC CAGTGACACC GAGTTCCACC CCATCGGCTT CCATATCTTC 
CAGGTCCCAG AGGGTGGAAG GAGCCAGGAC GCACCCCCAC TGCTGCTGCA GGAGCCGCTG 
CTGAGCTGCG TGCCACATCG CTACGCCCAG GAGGTGAGCC GGCTCTGCCT CCTGCCTGCG 
GGCACCTACA AGGTTGTGCC CTCCACCTAC CTGCCGGACA CAGAGGGGGC CTTCACAGTG 
ACCATCGCAA CCAGGATTGA CAGGCCATCC ATTCACAGCC AGGAGATGCT GGGCCAGTTC 
CTCCAAGAGG TCTCCATCAT GGCAGTGATG AAAACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_075571.2
CDS	179..2197
Translation	MRAGRGATPARELFRDAAFPAADSSLFCDLSTPLAQFREDITWRRPQEICATPRLFPDDPREGQVKQGLLGDCWFLCACAALQKSRHLLDQVIPPGQPSWADQEYRGSFTCRIWQFGRWVEVTTDDRLPCLAGRLCFSRCQREDVFWLPLLEKVYAKVHGSYEHLWAGQVADALVDLTGGLAERWNLKGVAGSGGQQDRPGRWEHRTCRQLLHLKDQCLISCCVLSPRAGARELGEFHAFIVSDLRELQGQAGQCILLLRIQNPWGRRCWQGLWREGGEGWSQVDAAVASELLSQLQEGEFWVEEEEFLREFDELTVGYPVTEAGHLQSLYTERLLCHTRALPGAWVKGQSAGGCRNNSGFPSNPKFWLRVSEPSEVYIAVLQRSRLHAADWAGRARALVGDSHTSWSPASIPGKHYQAVGLHLWKVEKRRVNLPRVLSMPPVAGTACHAYDREVHLRCELSPGYYLAVPSTFLKDAPGEFLLRVFSTGRVSLSAIRAVAKNTTPGAALPAGEWGTVQLRGSWRVGQTAGGSRNFASYPTNPCFPFSVPEGPGPRCVRITLHQHCRPSDTEFHPIGFHIFQVPEGGRSQDAPPLLLQEPLLSCVPHRYAQEVSRLCLLPAGTYKVVPSTYLPDTEGAFTVTIATRIDRPSIHSQEMLGQFLQEVSIMAVMKT

Target ORF information:

RefSeq Version	NM_023083.4
Organism	Homo sapiens(human)
Definition	Homo sapiens calpain 10 (CAPN10), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_023083.4

ORF Insert Sequence:

ATGCGGGCGG GCCGGGGCGC GACGCCGGCG AGGGAGCTGT TCCGGGACGC CGCCTTCCCC 
GCCGCGGACT CCTCGCTCTT CTGCGACTTG TCTACGCCGC TGGCCCAGTT CCGCGAGGAC 
ATCACGTGGA GGCGGCCCCA GGAGATTTGT GCCACACCCC GGCTGTTTCC AGATGACCCA 
CGGGAAGGGC AGGTGAAGCA GGGGCTGCTG GGGGATTGCT GGTTCCTGTG TGCCTGCGCC 
GCGCTGCAGA AGAGCAGGCA CCTCCTGGAC CAGGTCATTC CTCCGGGACA GCCGAGCTGG 
GCCGACCAGG AGTACCGGGG CTCCTTCACC TGTCGCATTT GGCAGTTTGG ACGCTGGGTG 
GAGGTGACCA CAGATGACCG CCTGCCGTGC CTTGCAGGGA GACTCTGTTT CTCCCGCTGC 
CAGAGGGAGG ATGTGTTCTG GCTCCCCTTA CTGGAAAAGG TCTACGCCAA GGTCCATGGG 
TCCTACGAGC ACCTGTGGGC CGGGCAGGTG GCGGATGCCC TGGTGGACCT GACCGGCGGC 
CTGGCAGAAA GATGGAACCT GAAGGGCGTA GCAGGAAGCG GAGGCCAGCA GGACAGGCCA 
GGCCGCTGGG AGCACAGGAC TTGTCGGCAG CTGCTCCACC TGAAGGACCA GTGTCTGATC 
AGCTGCTGCG TGCTCAGCCC CAGAGCAGGT GCCCGGGAGC TGGGGGAGTT CCATGCCTTC 
ATTGTCTCGG ACCTGCGGGA GCTCCAGGGT CAGGCGGGCC AGTGCATCCT GCTGCTGCGG 
ATCCAGAACC CCTGGGGCCG GCGGTGCTGG CAGGGGCTCT GGAGAGAGGG GGGTGAAGGG 
TGGAGCCAGG TAGATGCAGC GGTAGCATCT GAGCTCCTGT CCCAGCTCCA GGAAGGGGAG 
TTCTGGGTGG AGGAGGAGGA GTTCCTCAGG GAGTTTGACG AGCTCACCGT TGGCTACCCG 
GTCACGGAGG CCGGCCACCT GCAGAGCCTC TACACAGAGA GGCTGCTCTG CCATACGCGG 
GCGCTGCCTG GGGCCTGGGT CAAGGGCCAG TCAGCAGGAG GCTGCCGGAA CAACAGCGGC 
TTTCCCAGCA ACCCCAAATT CTGGCTGCGG GTCTCAGAAC CGAGTGAGGT GTACATTGCC 
GTCCTGCAGA GATCCAGGCT GCACGCGGCG GACTGGGCAG GCCGGGCCCG GGCACTGGTG 
GGTGACAGTC ATACTTCGTG GAGCCCAGCG AGCATCCCGG GCAAGCACTA CCAGGCTGTG 
GGTCTGCACC TCTGGAAGGT AGAGAAGCGG CGGGTCAATC TGCCTAGGGT CCTGTCCATG 
CCCCCCGTGG CTGGCACCGC GTGCCATGCA TACGACCGGG AGGTCCACCT GCGTTGTGAG 
CTCTCACCGG GCTACTACCT GGCTGTCCCC AGCACCTTCC TGAAGGACGC GCCAGGGGAG 
TTCCTGCTCC GAGTCTTCTC TACCGGGCGA GTCTCCCTTA GCGCCATCAG GGCAGTGGCC 
AAGAACACCA CCCCCGGGGC AGCCCTGCCT GCGGGGGAGT GGGGGACCGT GCAGCTACGG 
GGTTCTTGGA GAGTCGGCCA GACGGCGGGG GGCAGCAGGA ACTTTGCCTC ATACCCCACC 
AACCCCTGCT TCCCCTTCTC GGTCCCCGAG GGCCCTGGCC CCCGCTGCGT CCGCATCACT 
CTGCATCAGC ACTGCCGGCC CAGTGACACC GAGTTCCACC CCATCGGCTT CCATATCTTC 
CAGGTCCCAG AGGGTGGAAG GAGCCAGGAC GCACCCCCAC TGCTGCTGCA GGAGCCGCTG 
CTGAGCTGCG TGCCACATCG CTACGCCCAG GAGGTGAGCC GGCTCTGCCT CCTGCCTGCG 
GGCACCTACA AGGTTGTGCC CTCCACCTAC CTGCCGGACA CAGAGGGGGC CTTCACAGTG 
ACCATCGCAA CCAGGATTGA CAGGCCATCC ATTCACAGCC AGGAGATGCT GGGCCAGTTC 
CTCCAAGAGG TCTCCATCAT GGCAGTGATG AAAACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAPN10 ( NM_023085.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_075573.3 Homo sapiens calpain 10 (CAPN10), transcript variant 3, mRNA.

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CloneID	OHu108241
Clone ID Related Accession (Same CDS sequence)	NM_023085.4
Accession Version	NM_023085.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1554bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-20
Organism	Homo sapiens(human)
Product	calpain-10 isoform c
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC124862.4. On Jun 1, 2019 this sequence version replaced NM_023085.3. Transcript Variant: This variant (3, also known as CAPN10c) lacks two consecutive exons, resulting in the loss of an in-frame segment in the 3' coding region, compared to variant 1. The encoded isoform (c) is shorter than isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF089091.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2142586 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCGGGCGG GCCGGGGCGC GACGCCGGCG AGGGAGCTGT TCCGGGACGC CGCCTTCCCC 
GCCGCGGACT CCTCGCTCTT CTGCGACTTG TCTACGCCGC TGGCCCAGTT CCGCGAGGAC 
ATCACGTGGA GGCGGCCCCA GGAGATTTGT GCCACACCCC GGCTGTTTCC AGATGACCCA 
CGGGAAGGGC AGGTGAAGCA GGGGCTGCTG GGGGATTGCT GGTTCCTGTG TGCCTGCGCC 
GCGCTGCAGA AGAGCAGGCA CCTCCTGGAC CAGGTCATTC CTCCGGGACA GCCGAGCTGG 
GCCGACCAGG AGTACCGGGG CTCCTTCACC TGTCGCATTT GGCAGTTTGG ACGCTGGGTG 
GAGGTGACCA CAGATGACCG CCTGCCGTGC CTTGCAGGGA GACTCTGTTT CTCCCGCTGC 
CAGAGGGAGG ATGTGTTCTG GCTCCCCTTA CTGGAAAAGG TCTACGCCAA GGTCCATGGG 
TCCTACGAGC ACCTGTGGGC CGGGCAGGTG GCGGATGCCC TGGTGGACCT GACCGGCGGC 
CTGGCAGAAA GATGGAACCT GAAGGGCGTA GCAGGAAGCG GAGGCCAGCA GGACAGGCCA 
GGCCGCTGGG AGCACAGGAC TTGTCGGCAG CTGCTCCACC TGAAGGACCA GTGTCTGATC 
AGCTGCTGCG TGCTCAGCCC CAGAGCAGGT GCCCGGGAGC TGGGGGAGTT CCATGCCTTC 
ATTGTCTCGG ACCTGCGGGA GCTCCAGGGT CAGGCGGGCC AGTGCATCCT GCTGCTGCGG 
ATCCAGAACC CCTGGGGCCG GCGGTGCTGG CAGGGGCTCT GGAGAGAGGG GGGTGAAGGG 
TGGAGCCAGG TAGATGCAGC GGTAGCATCT GAGCTCCTGT CCCAGCTCCA GGAAGGGGAG 
TTCTGGGTGG AGGAGGAGGA GTTCCTCAGG GAGTTTGACG AGCTCACCGT TGGCTACCCG 
GTCACGGAGG CCGGCCACCT GCAGAGCCTC TACACAGAGA GGCTGCTCTG CCATACGCGG 
GCGCTGCCTG GGGCCTGGGT CAAGGGCCAG TCAGCAGGAG GCTGCCGGAA CAACAGCGGC 
TTTCCCAGCA ACCCCAAATT CTGGCTGCGG GTCTCAGAAC CGAGTGAGGT GTACATTGCC 
GTCCTGCAGA GATCCAGGCT GCACGCGGCG GACTGGGCAG GCCGGGCCCG GGCACTGGTG 
GGTGACAGTC ATACTTCGTG GAGCCCAGCG AGCATCCCGG GCAAGCACTA CCAGGCTGTG 
GGTCTGCACC TCTGGAAGGT CCCAGAGGGT GGAAGGAGCC AGGACGCACC CCCACTGCTG 
CTGCAGGAGC CGCTGCTGAG CTGCGTGCCA CATCGCTACG CCCAGGAGGT GAGCCGGCTC 
TGCCTCCTGC CTGCGGGCAC CTACAAGGTT GTGCCCTCCA CCTACCTGCC GGACACAGAG 
GGGGCCTTCA CAGTGACCAT CGCAACCAGG ATTGACAGGC CATCCATTCA CAGCCAGGAG 
ATGCTGGGCC AGTTCCTCCA AGAGGTCTCC ATCATGGCAG TGATGAAAAC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_075573.3
CDS	179..1732
Translation	MRAGRGATPARELFRDAAFPAADSSLFCDLSTPLAQFREDITWRRPQEICATPRLFPDDPREGQVKQGLLGDCWFLCACAALQKSRHLLDQVIPPGQPSWADQEYRGSFTCRIWQFGRWVEVTTDDRLPCLAGRLCFSRCQREDVFWLPLLEKVYAKVHGSYEHLWAGQVADALVDLTGGLAERWNLKGVAGSGGQQDRPGRWEHRTCRQLLHLKDQCLISCCVLSPRAGARELGEFHAFIVSDLRELQGQAGQCILLLRIQNPWGRRCWQGLWREGGEGWSQVDAAVASELLSQLQEGEFWVEEEEFLREFDELTVGYPVTEAGHLQSLYTERLLCHTRALPGAWVKGQSAGGCRNNSGFPSNPKFWLRVSEPSEVYIAVLQRSRLHAADWAGRARALVGDSHTSWSPASIPGKHYQAVGLHLWKVPEGGRSQDAPPLLLQEPLLSCVPHRYAQEVSRLCLLPAGTYKVVPSTYLPDTEGAFTVTIATRIDRPSIHSQEMLGQFLQEVSIMAVMKT

Target ORF information:

RefSeq Version	NM_023085.4
Organism	Homo sapiens(human)
Definition	Homo sapiens calpain 10 (CAPN10), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_023085.4

ORF Insert Sequence:

ATGCGGGCGG GCCGGGGCGC GACGCCGGCG AGGGAGCTGT TCCGGGACGC CGCCTTCCCC 
GCCGCGGACT CCTCGCTCTT CTGCGACTTG TCTACGCCGC TGGCCCAGTT CCGCGAGGAC 
ATCACGTGGA GGCGGCCCCA GGAGATTTGT GCCACACCCC GGCTGTTTCC AGATGACCCA 
CGGGAAGGGC AGGTGAAGCA GGGGCTGCTG GGGGATTGCT GGTTCCTGTG TGCCTGCGCC 
GCGCTGCAGA AGAGCAGGCA CCTCCTGGAC CAGGTCATTC CTCCGGGACA GCCGAGCTGG 
GCCGACCAGG AGTACCGGGG CTCCTTCACC TGTCGCATTT GGCAGTTTGG ACGCTGGGTG 
GAGGTGACCA CAGATGACCG CCTGCCGTGC CTTGCAGGGA GACTCTGTTT CTCCCGCTGC 
CAGAGGGAGG ATGTGTTCTG GCTCCCCTTA CTGGAAAAGG TCTACGCCAA GGTCCATGGG 
TCCTACGAGC ACCTGTGGGC CGGGCAGGTG GCGGATGCCC TGGTGGACCT GACCGGCGGC 
CTGGCAGAAA GATGGAACCT GAAGGGCGTA GCAGGAAGCG GAGGCCAGCA GGACAGGCCA 
GGCCGCTGGG AGCACAGGAC TTGTCGGCAG CTGCTCCACC TGAAGGACCA GTGTCTGATC 
AGCTGCTGCG TGCTCAGCCC CAGAGCAGGT GCCCGGGAGC TGGGGGAGTT CCATGCCTTC 
ATTGTCTCGG ACCTGCGGGA GCTCCAGGGT CAGGCGGGCC AGTGCATCCT GCTGCTGCGG 
ATCCAGAACC CCTGGGGCCG GCGGTGCTGG CAGGGGCTCT GGAGAGAGGG GGGTGAAGGG 
TGGAGCCAGG TAGATGCAGC GGTAGCATCT GAGCTCCTGT CCCAGCTCCA GGAAGGGGAG 
TTCTGGGTGG AGGAGGAGGA GTTCCTCAGG GAGTTTGACG AGCTCACCGT TGGCTACCCG 
GTCACGGAGG CCGGCCACCT GCAGAGCCTC TACACAGAGA GGCTGCTCTG CCATACGCGG 
GCGCTGCCTG GGGCCTGGGT CAAGGGCCAG TCAGCAGGAG GCTGCCGGAA CAACAGCGGC 
TTTCCCAGCA ACCCCAAATT CTGGCTGCGG GTCTCAGAAC CGAGTGAGGT GTACATTGCC 
GTCCTGCAGA GATCCAGGCT GCACGCGGCG GACTGGGCAG GCCGGGCCCG GGCACTGGTG 
GGTGACAGTC ATACTTCGTG GAGCCCAGCG AGCATCCCGG GCAAGCACTA CCAGGCTGTG 
GGTCTGCACC TCTGGAAGGT CCCAGAGGGT GGAAGGAGCC AGGACGCACC CCCACTGCTG 
CTGCAGGAGC CGCTGCTGAG CTGCGTGCCA CATCGCTACG CCCAGGAGGT GAGCCGGCTC 
TGCCTCCTGC CTGCGGGCAC CTACAAGGTT GTGCCCTCCA CCTACCTGCC GGACACAGAG 
GGGGCCTTCA CAGTGACCAT CGCAACCAGG ATTGACAGGC CATCCATTCA CAGCCAGGAG 
ATGCTGGGCC AGTTCCTCCA AGAGGTCTCC ATCATGGCAG TGATGAAAAC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAPN10 ( NM_023085.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_075573.2 Homo sapiens calpain 10 (CAPN10), transcript variant 3, mRNA.

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CloneID	OHu15864
Clone ID Related Accession (Same CDS sequence)	NM_023085.3
Accession Version	NM_023085.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1554bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-22
Organism	Homo sapiens(human)
Product	calpain-10 isoform c
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL558905.3, DA413480.1, AF089091.1, BQ962770.1, AK074807.1, AU310002.1 and BQ052841.1. On Sep 3, 2010 this sequence version replaced NM_023085.2. Transcript Variant: This variant (3, also known as CAPN10c) lacks two consecutive exons, resulting in the loss of an in-frame segment in the 3' coding region, compared to variant 1. The encoded isoform (c) is shorter than isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF089091.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2142586 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCGGGCGG GCCGGGGCGC GACGCCGGCG AGGGAGCTGT TCCGGGACGC CGCCTTCCCC 
GCCGCGGACT CCTCGCTCTT CTGCGACTTG TCTACGCCGC TGGCCCAGTT CCGCGAGGAC 
ATCACGTGGA GGCGGCCCCA GGAGATTTGT GCCACACCCC GGCTGTTTCC AGATGACCCA 
CGGGAAGGGC AGGTGAAGCA GGGGCTGCTG GGGGATTGCT GGTTCCTGTG TGCCTGCGCC 
GCGCTGCAGA AGAGCAGGCA CCTCCTGGAC CAGGTCATTC CTCCGGGACA GCCGAGCTGG 
GCCGACCAGG AGTACCGGGG CTCCTTCACC TGTCGCATTT GGCAGTTTGG ACGCTGGGTG 
GAGGTGACCA CAGATGACCG CCTGCCGTGC CTTGCAGGGA GACTCTGTTT CTCCCGCTGC 
CAGAGGGAGG ATGTGTTCTG GCTCCCCTTA CTGGAAAAGG TCTACGCCAA GGTCCATGGG 
TCCTACGAGC ACCTGTGGGC CGGGCAGGTG GCGGATGCCC TGGTGGACCT GACCGGCGGC 
CTGGCAGAAA GATGGAACCT GAAGGGCGTA GCAGGAAGCG GAGGCCAGCA GGACAGGCCA 
GGCCGCTGGG AGCACAGGAC TTGTCGGCAG CTGCTCCACC TGAAGGACCA GTGTCTGATC 
AGCTGCTGCG TGCTCAGCCC CAGAGCAGGT GCCCGGGAGC TGGGGGAGTT CCATGCCTTC 
ATTGTCTCGG ACCTGCGGGA GCTCCAGGGT CAGGCGGGCC AGTGCATCCT GCTGCTGCGG 
ATCCAGAACC CCTGGGGCCG GCGGTGCTGG CAGGGGCTCT GGAGAGAGGG GGGTGAAGGG 
TGGAGCCAGG TAGATGCAGC GGTAGCATCT GAGCTCCTGT CCCAGCTCCA GGAAGGGGAG 
TTCTGGGTGG AGGAGGAGGA GTTCCTCAGG GAGTTTGACG AGCTCACCGT TGGCTACCCG 
GTCACGGAGG CCGGCCACCT GCAGAGCCTC TACACAGAGA GGCTGCTCTG CCATACGCGG 
GCGCTGCCTG GGGCCTGGGT CAAGGGCCAG TCAGCAGGAG GCTGCCGGAA CAACAGCGGC 
TTTCCCAGCA ACCCCAAATT CTGGCTGCGG GTCTCAGAAC CGAGTGAGGT GTACATTGCC 
GTCCTGCAGA GATCCAGGCT GCACGCGGCG GACTGGGCAG GCCGGGCCCG GGCACTGGTG 
GGTGACAGTC ATACTTCGTG GAGCCCAGCG AGCATCCCGG GCAAGCACTA CCAGGCTGTG 
GGTCTGCACC TCTGGAAGGT CCCAGAGGGT GGAAGGAGCC AGGACGCACC CCCACTGCTG 
CTGCAGGAGC CGCTGCTGAG CTGCGTGCCA CATCGCTACG CCCAGGAGGT GAGCCGGCTC 
TGCCTCCTGC CTGCGGGCAC CTACAAGGTT GTGCCCTCCA CCTACCTGCC GGACACAGAG 
GGGGCCTTCA CAGTGACCAT CGCAACCAGG ATTGACAGGC CATCCATTCA CAGCCAGGAG 
ATGCTGGGCC AGTTCCTCCA AGAGGTCTCC GTCATGGCAG TGATGAAAAC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_075573.2
CDS	197..1750
Translation	MRAGRGATPARELFRDAAFPAADSSLFCDLSTPLAQFREDITWRRPQEICATPRLFPDDPREGQVKQGLLGDCWFLCACAALQKSRHLLDQVIPPGQPSWADQEYRGSFTCRIWQFGRWVEVTTDDRLPCLAGRLCFSRCQREDVFWLPLLEKVYAKVHGSYEHLWAGQVADALVDLTGGLAERWNLKGVAGSGGQQDRPGRWEHRTCRQLLHLKDQCLISCCVLSPRAGARELGEFHAFIVSDLRELQGQAGQCILLLRIQNPWGRRCWQGLWREGGEGWSQVDAAVASELLSQLQEGEFWVEEEEFLREFDELTVGYPVTEAGHLQSLYTERLLCHTRALPGAWVKGQSAGGCRNNSGFPSNPKFWLRVSEPSEVYIAVLQRSRLHAADWAGRARALVGDSHTSWSPASIPGKHYQAVGLHLWKVPEGGRSQDAPPLLLQEPLLSCVPHRYAQEVSRLCLLPAGTYKVVPSTYLPDTEGAFTVTIATRIDRPSIHSQEMLGQFLQEVSVMAVMKT

Target ORF information:

RefSeq Version	NM_023085.3
Organism	Homo sapiens(human)
Definition	Homo sapiens calpain 10 (CAPN10), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_023085.3

ORF Insert Sequence:

ATGCGGGCGG GCCGGGGCGC GACGCCGGCG AGGGAGCTGT TCCGGGACGC CGCCTTCCCC 
GCCGCGGACT CCTCGCTCTT CTGCGACTTG TCTACGCCGC TGGCCCAGTT CCGCGAGGAC 
ATCACGTGGA GGCGGCCCCA GGAGATTTGT GCCACACCCC GGCTGTTTCC AGATGACCCA 
CGGGAAGGGC AGGTGAAGCA GGGGCTGCTG GGGGATTGCT GGTTCCTGTG TGCCTGCGCC 
GCGCTGCAGA AGAGCAGGCA CCTCCTGGAC CAGGTCATTC CTCCGGGACA GCCGAGCTGG 
GCCGACCAGG AGTACCGGGG CTCCTTCACC TGTCGCATTT GGCAGTTTGG ACGCTGGGTG 
GAGGTGACCA CAGATGACCG CCTGCCGTGC CTTGCAGGGA GACTCTGTTT CTCCCGCTGC 
CAGAGGGAGG ATGTGTTCTG GCTCCCCTTA CTGGAAAAGG TCTACGCCAA GGTCCATGGG 
TCCTACGAGC ACCTGTGGGC CGGGCAGGTG GCGGATGCCC TGGTGGACCT GACCGGCGGC 
CTGGCAGAAA GATGGAACCT GAAGGGCGTA GCAGGAAGCG GAGGCCAGCA GGACAGGCCA 
GGCCGCTGGG AGCACAGGAC TTGTCGGCAG CTGCTCCACC TGAAGGACCA GTGTCTGATC 
AGCTGCTGCG TGCTCAGCCC CAGAGCAGGT GCCCGGGAGC TGGGGGAGTT CCATGCCTTC 
ATTGTCTCGG ACCTGCGGGA GCTCCAGGGT CAGGCGGGCC AGTGCATCCT GCTGCTGCGG 
ATCCAGAACC CCTGGGGCCG GCGGTGCTGG CAGGGGCTCT GGAGAGAGGG GGGTGAAGGG 
TGGAGCCAGG TAGATGCAGC GGTAGCATCT GAGCTCCTGT CCCAGCTCCA GGAAGGGGAG 
TTCTGGGTGG AGGAGGAGGA GTTCCTCAGG GAGTTTGACG AGCTCACCGT TGGCTACCCG 
GTCACGGAGG CCGGCCACCT GCAGAGCCTC TACACAGAGA GGCTGCTCTG CCATACGCGG 
GCGCTGCCTG GGGCCTGGGT CAAGGGCCAG TCAGCAGGAG GCTGCCGGAA CAACAGCGGC 
TTTCCCAGCA ACCCCAAATT CTGGCTGCGG GTCTCAGAAC CGAGTGAGGT GTACATTGCC 
GTCCTGCAGA GATCCAGGCT GCACGCGGCG GACTGGGCAG GCCGGGCCCG GGCACTGGTG 
GGTGACAGTC ATACTTCGTG GAGCCCAGCG AGCATCCCGG GCAAGCACTA CCAGGCTGTG 
GGTCTGCACC TCTGGAAGGT CCCAGAGGGT GGAAGGAGCC AGGACGCACC CCCACTGCTG 
CTGCAGGAGC CGCTGCTGAG CTGCGTGCCA CATCGCTACG CCCAGGAGGT GAGCCGGCTC 
TGCCTCCTGC CTGCGGGCAC CTACAAGGTT GTGCCCTCCA CCTACCTGCC GGACACAGAG 
GGGGCCTTCA CAGTGACCAT CGCAACCAGG ATTGACAGGC CATCCATTCA CAGCCAGGAG 
ATGCTGGGCC AGTTCCTCCA AGAGGTCTCC GTCATGGCAG TGATGAAAAC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Correlation between Calpain-10 single-nucleotide polymorphisms and obstructive sleep apnea/hypopnoea syndrome with ischemic stroke in a Chinese population: A population-based study.
Medicine96(16)e6570(2017 Apr)
Zhang W,Zhao ZR,Dai CF,Zhang R,Chen J,Tian HJ,Wang YL,Sun JH,Lian QF

Single-nucleotide polymorphism of INS, INSR, IRS1, IRS2, PPAR-G and CAPN10 genes in the pathogenesis of polycystic ovary syndrome.
Journal of genetics96(1)87-96(2017 Mar)
Thangavelu M,Godla UR,Paul Solomon FD,Maddaly R

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CAPN10 cDNA ORF clone, Homo sapiens(human)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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