ZWINT cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	ZWINT
Entrez Gene ID	11130
Full Name	ZW10 interacting kinetochore protein
Synonyms	HZwint-1,KNTC2AP,SIP30,ZWINT1
General protein information	Preferred Names ZW10 interacting kinetochore protein Names ZW10 interactor SNAP25 interacting protein of 30 kDa ZW10 interactor, kinetochore protein human ZW10 interacting protein-1 zwint-1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 10 Map Location: 10q21.1
Summary	This gene encodes a protein that is clearly involved in kinetochore function although an exact role is not known. It interacts with ZW10, another kinetochore protein, possibly regulating the association between ZW10 and kinetochores. The encoded protein localizes to prophase kinetochores before ZW10 does and it remains detectable on the kinetochore until late anaphase. It has a uniform distribution in the cytoplasm of interphase cells. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008].
Disorder MIM:	609177

mRNA and Protein(s)

mRNA	Protein	Name
NM_032997.2	NP_127490.1	ZW10 interactor isoform a
XM_024447784.1	XP_024303552.1	ZW10 interactor isoform X1
XM_017015605.1	XP_016871094.1	ZW10 interactor isoform X1
NM_001005413.1	NP_001005413.1	ZW10 interactor isoform b
NM_007057.4	NP_008988.2	ZW10 interactor isoform a
XM_017015605.2	XP_016871094.1	ZW10 interactor isoform X2
NM_007057.3	NP_008988.2	ZW10 interactor isoform a

Pathways from BioSystems

Homologies

Bos taurus (cattle)	LOC101904477	XP_005206403.1
Homo sapiens (human)	ZWINT	NP_127490.1
Pan troglodytes (chimpanzee)	ZWINT	XP_001163535.2
	ZWINT	XP_001098136.2
Canis lupus familiaris (dog)	LOC477579	XP_005636720.1
Bos taurus (cattle)	ZWINT	NP_001035619.1
Rattus norvegicus (Norway rat)	Zwint	NP_671479.1
Mus musculus (house mouse)	Zwint	NP_079911.1

Gene Ontology
Bibliography

Related articles in PubMed

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

Zwint-1 is required for spindle assembly checkpoint function and kinetochore-microtubule attachment during oocyte meiosis.
Woo Seo D, Yeop You S, Chung WJ, Cho DH, Kim JS, Su Oh J
Scientific reports515431(2015 Oct)

A human interactome in three quantitative dimensions organized by stoichiometries and abundances.
Hein MY, Hubner NC, Poser I, Cox J, Nagaraj N, Toyoda Y, Gak IA, Weisswange I, Mansfeld J, Buchholz F, Hyman AA, Mann M
Cell163(3)712-23(2015 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Our results provide the first evidence that Zwint-1 is required to correct erroneous kinetochore-microtubule attachment and regulate spindle checkpoint function during meiosis.
Title: Zwint-1 is required for spindle assembly checkpoint function and kinetochore-microtubule attachment during oocyte meiosis.

the E3 ubiquitin ligase terf causes protein degradation of ZWINT and negatively regulates cell proliferation
Title: Terf/TRIM17 stimulates degradation of kinetochore protein ZWINT and regulates cell proliferation.

These studies identify zwint-1 as a novel AurB substrate required for kinetochore assembly and for proper spindle assembly checkpoint silencing at metaphase
Title: Zwint-1 is a novel Aurora B substrate required for the assembly of a dynein-binding platform on kinetochores.

hZwint-1 bridges the inner and outer kinetochore: identification of the kinetochore localization domain and the hZw10-interaction domain
Title: hZwint-1 bridges the inner and outer kinetochore: identification of the kinetochore localization domain and the hZw10-interaction domain.

Observational study of gene-disease association. (HuGE Navigator)
Title: Single nucleotide polymorphisms in chromosomal instability genes and risk and clinical outcome of breast cancer: a Swedish prospective case-control study.

The following ZWINT gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ZWINT cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu00013	NM_032997.2 Latest version!	Homo sapiens ZW10 interacting kinetochore protein (ZWINT), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$216.30 $309.00
OHu00013	NM_007057.4 Latest version!	Homo sapiens ZW10 interacting kinetochore protein (ZWINT), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$216.30 $309.00
OHu00015	NM_001005413.1 Latest version!	Homo sapiens ZW10 interacting kinetochore protein (ZWINT), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu106418	XM_024447784.1 Latest version!	Homo sapiens ZW10 interacting kinetochore protein (ZWINT), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OHu91045	XM_017015605.2 Latest version!	Homo sapiens ZW10 interacting kinetochore protein (ZWINT), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
View Old Accession Versions >>
OHu91045	XM_017015605.1	Homo sapiens ZW10 interacting kinetochore protein (ZWINT), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OHu00013	NM_007057.3	Homo sapiens ZW10 interacting kinetochore protein (ZWINT), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$216.30 $309.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ZWINT ( NM_032997.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_127490.1 Homo sapiens ZW10 interacting kinetochore protein (ZWINT), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu00013
Clone ID Related Accession (Same CDS sequence)	NM_032997.2 , NM_007057.4 , NM_007057.3
Accession Version	NM_032997.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 834bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	ZW10 interactor isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BG615304.1, BC020979.1, BC000411.1 and BC016357.2. On Oct 1, 2004 this sequence version replaced NM_032997.1. Transcript Variant: This variant (2) differs in the 3' UTR compared to variant 1. Variants 1 and 2 both encode isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163657.303742.1, SRR1163658.245280.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2150385, SAMEA2161836 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGGCAG CGGAGACAGA GGCGGAAGCT GCAGCCCTAG AGGTCCTGGC TGAGGTGGCA 
GGCATCTTGG AACCTGTAGG CCTGCAGGAG GAGGCAGAAC TGCCAGCCAA GATCCTGGTT 
GAGTTTGTGG TGGACTCTCA GAAGAAAGAC AAGCTGCTCT GCAGCCAGCT TCAGGTAGCG 
GATTTCCTGC AGAACATCCT GGCTCAGGAG GACACTGCTA AGGGTCTCGA CCCCTTGGCT 
TCTGAAGACA CGAGCCGACA GAAGGCAATT GCAGCTAAGG AACAATGGAA AGAGCTGAAG 
GCCACCTACA GGGAGCACGT AGAGGCCATC AAAATTGGCC TCACCAAGGC CCTGACTCAG 
ATGGAGGAAG CCCAGAGGAA ACGGACACAA CTCCGGGAAG CCTTTGAGCA GCTCCAGGCC 
AAGAAACAAA TGGCCATGGA GAAACGCAGA GCAGTCCAGA ACCAGTGGCA GCTACAACAG 
GAGAAGCATC TGCAGCATCT GGCGGAGGTT TCTGCAGAGG TGAGGGAGCG TAAGACAGGG 
ACTCAGCAGG AGCTTGACAG GGTGTTTCAG AAACTTGGAA ACCTGAAGCA GCAGGCAGAA 
CAGGAGCGGG ACAAGCTGCA GAGGTATCAG ACCTTCCTCC AGCTTCTGTA TACCCTGCAG 
GGTAAGCTGT TGTTCCCTGA GGCTGAGGCT GAGGCAGAGA ATCTTCCAGA TGATAAACCC 
CAGCAGCCGA CTCGACCCCA GGAGCAGAGT ACAGGAGACA CCATGGGGAG AGACCCTGGT 
GTGTCCTTCA AGGCTGTTGG TCTACAACCT GCTGGAGATG TAAATTTGCC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_127490.1
CDS	38..871
Translation	MEAAETEAEAAALEVLAEVAGILEPVGLQEEAELPAKILVEFVVDSQKKDKLLCSQLQVADFLQNILAQEDTAKGLDPLASEDTSRQKAIAAKEQWKELKATYREHVEAIKIGLTKALTQMEEAQRKRTQLREAFEQLQAKKQMAMEKRRAVQNQWQLQQEKHLQHLAEVSAEVRERKTGTQQELDRVFQKLGNLKQQAEQERDKLQRYQTFLQLLYTLQGKLLFPEAEAEAENLPDDKPQQPTRPQEQSTGDTMGRDPGVSFKAVGLQPAGDVNLP

Target ORF information:

RefSeq Version	NM_032997.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ZW10 interacting kinetochore protein (ZWINT), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_032997.2

ORF Insert Sequence:

ATGGAGGCAG CGGAGACAGA GGCGGAAGCT GCAGCCCTAG AGGTCCTGGC TGAGGTGGCA 
GGCATCTTGG AACCTGTAGG CCTGCAGGAG GAGGCAGAAC TGCCAGCCAA GATCCTGGTT 
GAGTTTGTGG TGGACTCTCA GAAGAAAGAC AAGCTGCTCT GCAGCCAGCT TCAGGTAGCG 
GATTTCCTGC AGAACATCCT GGCTCAGGAG GACACTGCTA AGGGTCTCGA CCCCTTGGCT 
TCTGAAGACA CGAGCCGACA GAAGGCAATT GCAGCTAAGG AACAATGGAA AGAGCTGAAG 
GCCACCTACA GGGAGCACGT AGAGGCCATC AAAATTGGCC TCACCAAGGC CCTGACTCAG 
ATGGAGGAAG CCCAGAGGAA ACGGACACAA CTCCGGGAAG CCTTTGAGCA GCTCCAGGCC 
AAGAAACAAA TGGCCATGGA GAAACGCAGA GCAGTCCAGA ACCAGTGGCA GCTACAACAG 
GAGAAGCATC TGCAGCATCT GGCGGAGGTT TCTGCAGAGG TGAGGGAGCG TAAGACAGGG 
ACTCAGCAGG AGCTTGACAG GGTGTTTCAG AAACTTGGAA ACCTGAAGCA GCAGGCAGAA 
CAGGAGCGGG ACAAGCTGCA GAGGTATCAG ACCTTCCTCC AGCTTCTGTA TACCCTGCAG 
GGTAAGCTGT TGTTCCCTGA GGCTGAGGCT GAGGCAGAGA ATCTTCCAGA TGATAAACCC 
CAGCAGCCGA CTCGACCCCA GGAGCAGAGT ACAGGAGACA CCATGGGGAG AGACCCTGGT 
GTGTCCTTCA AGGCTGTTGG TCTACAACCT GCTGGAGATG TAAATTTGCC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZWINT ( NM_007057.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_008988.2 Homo sapiens ZW10 interacting kinetochore protein (ZWINT), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu00013
Clone ID Related Accession (Same CDS sequence)	NM_032997.2 , NM_007057.4 , NM_007057.3
Accession Version	NM_007057.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 834bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	ZW10 interactor isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BG615304.1, BC020979.1, BC000411.1, BC016357.2 and AC010996.9. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_007057.3. Transcript Variant: This variant (1) is the predominant transcript and differs in the 3' UTR compared to variant 2. Variants 1 and 2 both encode isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR5189661.185059.1, SRR5189667.148865.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2150385, SAMEA2161836 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000373944.8/ ENSP00000363055.3 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGAGGCAG CGGAGACAGA GGCGGAAGCT GCAGCCCTAG AGGTCCTGGC TGAGGTGGCA 
GGCATCTTGG AACCTGTAGG CCTGCAGGAG GAGGCAGAAC TGCCAGCCAA GATCCTGGTT 
GAGTTTGTGG TGGACTCTCA GAAGAAAGAC AAGCTGCTCT GCAGCCAGCT TCAGGTAGCG 
GATTTCCTGC AGAACATCCT GGCTCAGGAG GACACTGCTA AGGGTCTCGA CCCCTTGGCT 
TCTGAAGACA CGAGCCGACA GAAGGCAATT GCAGCTAAGG AACAATGGAA AGAGCTGAAG 
GCCACCTACA GGGAGCACGT AGAGGCCATC AAAATTGGCC TCACCAAGGC CCTGACTCAG 
ATGGAGGAAG CCCAGAGGAA ACGGACACAA CTCCGGGAAG CCTTTGAGCA GCTCCAGGCC 
AAGAAACAAA TGGCCATGGA GAAACGCAGA GCAGTCCAGA ACCAGTGGCA GCTACAACAG 
GAGAAGCATC TGCAGCATCT GGCGGAGGTT TCTGCAGAGG TGAGGGAGCG TAAGACAGGG 
ACTCAGCAGG AGCTTGACAG GGTGTTTCAG AAACTTGGAA ACCTGAAGCA GCAGGCAGAA 
CAGGAGCGGG ACAAGCTGCA GAGGTATCAG ACCTTCCTCC AGCTTCTGTA TACCCTGCAG 
GGTAAGCTGT TGTTCCCTGA GGCTGAGGCT GAGGCAGAGA ATCTTCCAGA TGATAAACCC 
CAGCAGCCGA CTCGACCCCA GGAGCAGAGT ACAGGAGACA CCATGGGGAG AGACCCTGGT 
GTGTCCTTCA AGGCTGTTGG TCTACAACCT GCTGGAGATG TAAATTTGCC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_008988.2
CDS	24..857
Translation	MEAAETEAEAAALEVLAEVAGILEPVGLQEEAELPAKILVEFVVDSQKKDKLLCSQLQVADFLQNILAQEDTAKGLDPLASEDTSRQKAIAAKEQWKELKATYREHVEAIKIGLTKALTQMEEAQRKRTQLREAFEQLQAKKQMAMEKRRAVQNQWQLQQEKHLQHLAEVSAEVRERKTGTQQELDRVFQKLGNLKQQAEQERDKLQRYQTFLQLLYTLQGKLLFPEAEAEAENLPDDKPQQPTRPQEQSTGDTMGRDPGVSFKAVGLQPAGDVNLP

Target ORF information:

RefSeq Version	NM_007057.4
Organism	Homo sapiens(human)
Definition	Homo sapiens ZW10 interacting kinetochore protein (ZWINT), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007057.4

ORF Insert Sequence:

ATGGAGGCAG CGGAGACAGA GGCGGAAGCT GCAGCCCTAG AGGTCCTGGC TGAGGTGGCA 
GGCATCTTGG AACCTGTAGG CCTGCAGGAG GAGGCAGAAC TGCCAGCCAA GATCCTGGTT 
GAGTTTGTGG TGGACTCTCA GAAGAAAGAC AAGCTGCTCT GCAGCCAGCT TCAGGTAGCG 
GATTTCCTGC AGAACATCCT GGCTCAGGAG GACACTGCTA AGGGTCTCGA CCCCTTGGCT 
TCTGAAGACA CGAGCCGACA GAAGGCAATT GCAGCTAAGG AACAATGGAA AGAGCTGAAG 
GCCACCTACA GGGAGCACGT AGAGGCCATC AAAATTGGCC TCACCAAGGC CCTGACTCAG 
ATGGAGGAAG CCCAGAGGAA ACGGACACAA CTCCGGGAAG CCTTTGAGCA GCTCCAGGCC 
AAGAAACAAA TGGCCATGGA GAAACGCAGA GCAGTCCAGA ACCAGTGGCA GCTACAACAG 
GAGAAGCATC TGCAGCATCT GGCGGAGGTT TCTGCAGAGG TGAGGGAGCG TAAGACAGGG 
ACTCAGCAGG AGCTTGACAG GGTGTTTCAG AAACTTGGAA ACCTGAAGCA GCAGGCAGAA 
CAGGAGCGGG ACAAGCTGCA GAGGTATCAG ACCTTCCTCC AGCTTCTGTA TACCCTGCAG 
GGTAAGCTGT TGTTCCCTGA GGCTGAGGCT GAGGCAGAGA ATCTTCCAGA TGATAAACCC 
CAGCAGCCGA CTCGACCCCA GGAGCAGAGT ACAGGAGACA CCATGGGGAG AGACCCTGGT 
GTGTCCTTCA AGGCTGTTGG TCTACAACCT GCTGGAGATG TAAATTTGCC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZWINT ( NM_007057.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_008988.2 Homo sapiens ZW10 interacting kinetochore protein (ZWINT), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu00013
Clone ID Related Accession (Same CDS sequence)	NM_032997.2 , NM_007057.4 , NM_007057.3
Accession Version	NM_007057.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 834bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-30
Organism	Homo sapiens(human)
Product	ZW10 interactor isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BG615304.1, BC020979.1, BC000411.1 and BC016357.2. This sequence is a reference standard in the RefSeqGene project. On Oct 1, 2004 this sequence version replaced NM_007057.2. Transcript Variant: This variant (1) is the predominant transcript and differs in the 3' UTR compared to variant 2. Variants 1 and 2 both encode isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163655.676694.1, BC110399.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2150385, SAMEA2161836 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGGCAG CGGAGACAGA GGCGGAAGCT GCAGCCCTAG AGGTCCTGGC TGAGGTGGCA 
GGCATCTTGG AACCTGTAGG CCTGCAGGAG GAGGCAGAAC TGCCAGCCAA GATCCTGGTT 
GAGTTTGTGG TGGACTCTCA GAAGAAAGAC AAGCTGCTCT GCAGCCAGCT TCAGGTAGCG 
GATTTCCTGC AGAACATCCT GGCTCAGGAG GACACTGCTA AGGGTCTCGA CCCCTTGGCT 
TCTGAAGACA CGAGCCGACA GAAGGCAATT GCAGCTAAGG AACAATGGAA AGAGCTGAAG 
GCCACCTACA GGGAGCACGT AGAGGCCATC AAAATTGGCC TCACCAAGGC CCTGACTCAG 
ATGGAGGAAG CCCAGAGGAA ACGGACACAA CTCCGGGAAG CCTTTGAGCA GCTCCAGGCC 
AAGAAACAAA TGGCCATGGA GAAACGCAGA GCAGTCCAGA ACCAGTGGCA GCTACAACAG 
GAGAAGCATC TGCAGCATCT GGCGGAGGTT TCTGCAGAGG TGAGGGAGCG TAAGACAGGG 
ACTCAGCAGG AGCTTGACAG GGTGTTTCAG AAACTTGGAA ACCTGAAGCA GCAGGCAGAA 
CAGGAGCGGG ACAAGCTGCA GAGGTATCAG ACCTTCCTCC AGCTTCTGTA TACCCTGCAG 
GGTAAGCTGT TGTTCCCTGA GGCTGAGGCT GAGGCAGAGA ATCTTCCAGA TGATAAACCC 
CAGCAGCCGA CTCGACCCCA GGAGCAGAGT ACAGGAGACA CCATGGGGAG AGACCCTGGT 
GTGTCCTTCA AGGCTGTTGG TCTACAACCT GCTGGAGATG TAAATTTGCC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_008988.2
CDS	38..871
Translation	MEAAETEAEAAALEVLAEVAGILEPVGLQEEAELPAKILVEFVVDSQKKDKLLCSQLQVADFLQNILAQEDTAKGLDPLASEDTSRQKAIAAKEQWKELKATYREHVEAIKIGLTKALTQMEEAQRKRTQLREAFEQLQAKKQMAMEKRRAVQNQWQLQQEKHLQHLAEVSAEVRERKTGTQQELDRVFQKLGNLKQQAEQERDKLQRYQTFLQLLYTLQGKLLFPEAEAEAENLPDDKPQQPTRPQEQSTGDTMGRDPGVSFKAVGLQPAGDVNLP

Target ORF information:

RefSeq Version	NM_007057.3
Organism	Homo sapiens(human)
Definition	Homo sapiens ZW10 interacting kinetochore protein (ZWINT), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007057.3

ORF Insert Sequence:

ATGGAGGCAG CGGAGACAGA GGCGGAAGCT GCAGCCCTAG AGGTCCTGGC TGAGGTGGCA 
GGCATCTTGG AACCTGTAGG CCTGCAGGAG GAGGCAGAAC TGCCAGCCAA GATCCTGGTT 
GAGTTTGTGG TGGACTCTCA GAAGAAAGAC AAGCTGCTCT GCAGCCAGCT TCAGGTAGCG 
GATTTCCTGC AGAACATCCT GGCTCAGGAG GACACTGCTA AGGGTCTCGA CCCCTTGGCT 
TCTGAAGACA CGAGCCGACA GAAGGCAATT GCAGCTAAGG AACAATGGAA AGAGCTGAAG 
GCCACCTACA GGGAGCACGT AGAGGCCATC AAAATTGGCC TCACCAAGGC CCTGACTCAG 
ATGGAGGAAG CCCAGAGGAA ACGGACACAA CTCCGGGAAG CCTTTGAGCA GCTCCAGGCC 
AAGAAACAAA TGGCCATGGA GAAACGCAGA GCAGTCCAGA ACCAGTGGCA GCTACAACAG 
GAGAAGCATC TGCAGCATCT GGCGGAGGTT TCTGCAGAGG TGAGGGAGCG TAAGACAGGG 
ACTCAGCAGG AGCTTGACAG GGTGTTTCAG AAACTTGGAA ACCTGAAGCA GCAGGCAGAA 
CAGGAGCGGG ACAAGCTGCA GAGGTATCAG ACCTTCCTCC AGCTTCTGTA TACCCTGCAG 
GGTAAGCTGT TGTTCCCTGA GGCTGAGGCT GAGGCAGAGA ATCTTCCAGA TGATAAACCC 
CAGCAGCCGA CTCGACCCCA GGAGCAGAGT ACAGGAGACA CCATGGGGAG AGACCCTGGT 
GTGTCCTTCA AGGCTGTTGG TCTACAACCT GCTGGAGATG TAAATTTGCC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZWINT ( NM_001005413.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001005413.1 Homo sapiens ZW10 interacting kinetochore protein (ZWINT), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu00015
Clone ID Related Accession (Same CDS sequence)	NM_001005413.1
Accession Version	NM_001005413.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 693bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-10
Organism	Homo sapiens(human)
Product	ZW10 interactor isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BG615304.1, BM724168.1, BC020979.1 and BC016357.2. Transcript Variant: This variant (3) lacks an alternate in-frame segment compared to variant 1. The resulting isoform (b) has the same N- and C-termini but is shorter compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163655.581548.1, SRR1163658.199937.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2150385, SAMEA2161836 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGGCAG CGGAGACAGA GGCGGAAGCT GCAGCCCTAG AGGTCCTGGC TGAGGTGGCA 
GGCATCTTGG AACCTGTAGG CCTGCAGGAG GAGGCAGAAC TGCCAGCCAA GATCCTGGTT 
GAGTTTGTGG TGGACTCTCA GAAGAAAGAC AAGCTGCTCT GCAGCCAGCT TCAGGTAGCG 
GATTTCCTGC AGAACATCCT GGCTCAGGAG GACACTGCTA AGGGTCTCGA CCCCTTGGCT 
TCTGAAGACA CGAGCCGACA GAAGGCAATT GCAGCTAAGG AACAATGGAA AGAGCTGAAG 
GCCACCTACA GGGAGCACGT AGAGGCCATC AAAATTGGCC TCACCAAGGC CCTGACTCAG 
ATGGAGGAAG CCCAGAGGAA ACGGACACAA CTCCGGGAAG CCTTTGAGCA GCTCCAGGCC 
AAGAAACAAA TGGCCATGGA GAAACGCAGA GCAGTCCAGA ACCAGTGGCA GCTACAACAG 
GAGAAGCATC TGCAGCATCT GGCGGAGGTT TCTGCAGAGG GTAAGCTGTT GTTCCCTGAG 
GCTGAGGCTG AGGCAGAGAA TCTTCCAGAT GATAAACCCC AGCAGCCGAC TCGACCCCAG 
GAGCAGAGTA CAGGAGACAC CATGGGGAGA GACCCTGGTG TGTCCTTCAA GGCTGTTGGT 
CTACAACCTG CTGGAGATGT AAATTTGCCA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001005413.1
CDS	38..730
Translation	MEAAETEAEAAALEVLAEVAGILEPVGLQEEAELPAKILVEFVVDSQKKDKLLCSQLQVADFLQNILAQEDTAKGLDPLASEDTSRQKAIAAKEQWKELKATYREHVEAIKIGLTKALTQMEEAQRKRTQLREAFEQLQAKKQMAMEKRRAVQNQWQLQQEKHLQHLAEVSAEGKLLFPEAEAEAENLPDDKPQQPTRPQEQSTGDTMGRDPGVSFKAVGLQPAGDVNLP

Target ORF information:

RefSeq Version	NM_001005413.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ZW10 interacting kinetochore protein (ZWINT), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001005413.1

ORF Insert Sequence:

ATGGAGGCAG CGGAGACAGA GGCGGAAGCT GCAGCCCTAG AGGTCCTGGC TGAGGTGGCA 
GGCATCTTGG AACCTGTAGG CCTGCAGGAG GAGGCAGAAC TGCCAGCCAA GATCCTGGTT 
GAGTTTGTGG TGGACTCTCA GAAGAAAGAC AAGCTGCTCT GCAGCCAGCT TCAGGTAGCG 
GATTTCCTGC AGAACATCCT GGCTCAGGAG GACACTGCTA AGGGTCTCGA CCCCTTGGCT 
TCTGAAGACA CGAGCCGACA GAAGGCAATT GCAGCTAAGG AACAATGGAA AGAGCTGAAG 
GCCACCTACA GGGAGCACGT AGAGGCCATC AAAATTGGCC TCACCAAGGC CCTGACTCAG 
ATGGAGGAAG CCCAGAGGAA ACGGACACAA CTCCGGGAAG CCTTTGAGCA GCTCCAGGCC 
AAGAAACAAA TGGCCATGGA GAAACGCAGA GCAGTCCAGA ACCAGTGGCA GCTACAACAG 
GAGAAGCATC TGCAGCATCT GGCGGAGGTT TCTGCAGAGG GTAAGCTGTT GTTCCCTGAG 
GCTGAGGCTG AGGCAGAGAA TCTTCCAGAT GATAAACCCC AGCAGCCGAC TCGACCCCAG 
GAGCAGAGTA CAGGAGACAC CATGGGGAGA GACCCTGGTG TGTCCTTCAA GGCTGTTGGT 
CTACAACCTG CTGGAGATGT AAATTTGCCA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZWINT ( XM_024447784.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024303552.1 Homo sapiens ZW10 interacting kinetochore protein (ZWINT), transcript variant X1, mRNA.

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CloneID	OHu106418
Clone ID Related Accession (Same CDS sequence)	XM_024447784.1
Accession Version	XM_024447784.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 930bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ZW10 interactor isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCAG CGGAGACAGA GGCGGAAGCT GCAGCCCTAG AGGTCCTGGC TGAGGTGGCA 
GGCATCTTGG AACCTGTAGG CCTGCAGGAG GAGGCAGAAC TGCCAGCCAA GATCCTGGTT 
GAGTTTGTGG TGGACTCTCA GAAGAAAGAC AAGCTGCTCT GCAGCCAGCT TCAGGTAGCG 
GATTTCCTGC AGAACATCCT GGCTCAGGAG GACACTGCTA AGGGTCTCGA CCCCTTGGCT 
TCTGAAGACA CGAGCCGACA GAAGGCAATT GCAGCTAAGG AACAATGGAA AGAGCTGAAG 
GCCACCTACA GGGAGCACGT AGAGGCCATC AAAATTGGCC TCACCAAGGC CCTGACTCAG 
ATGGAGGAAG CCCAGAGGAA ACGGACACAA CTCCGGGAAG CCTTTGAGCA GCTCCAGGCC 
AAGAAACAAA TGGCCATGGA GAAACGCAGA GCAGTCCAGA ACCAGTGGCA GCTACAACAG 
GAGAAGCATC TGCAGCATCT GGCGGAGGTT TCTGCAGAGG TGAGGGAGCG TAAGACAGGG 
ACTCAGCAGG AGCTTGACAG GGTGTTTCAG AAACTTGGAA ACCTGAAGCA GCAGGCAGAA 
CAGGAGCGGG ACAAGCTGCA GAGGTATCAG ACCTTCCTCC AGCTTCTGTA TACCCTGCAG 
GGTAAGCTGT TGTTCCCTGA GGCTGAGGCT GAGGCAGAGA ATCTTCCAGA TGATAAACCC 
CAGCAGCCGA CTCGACCCCA GGAGCAGAGT ACAGGAGACA CCATGGGGAG AGACCCTGGT 
GTGTCCTTCA AGGTAAATGA GAGATGGAAC AGGTGGGCAT GGGCTGGCAG GTGGAGACAG 
AAGCTGGGAG ATTGGCCTTA CCCACTGTCT ATACTGTATT TCTCCAAGGC TGTTGGTCTA 
CAACCTGCTG GAGATGTAAA TTTGCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024303552.1
CDS	37..966
Translation	MEAAETEAEAAALEVLAEVAGILEPVGLQEEAELPAKILVEFVVDSQKKDKLLCSQLQVADFLQNILAQEDTAKGLDPLASEDTSRQKAIAAKEQWKELKATYREHVEAIKIGLTKALTQMEEAQRKRTQLREAFEQLQAKKQMAMEKRRAVQNQWQLQQEKHLQHLAEVSAEVRERKTGTQQELDRVFQKLGNLKQQAEQERDKLQRYQTFLQLLYTLQGKLLFPEAEAEAENLPDDKPQQPTRPQEQSTGDTMGRDPGVSFKVNERWNRWAWAGRWRQKLGDWPYPLSILYFSKAVGLQPAGDVNLP

Target ORF information:

RefSeq Version	XM_024447784.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ZW10 interacting kinetochore protein (ZWINT), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024447784.1

ORF Insert Sequence:

ATGGAGGCAG CGGAGACAGA GGCGGAAGCT GCAGCCCTAG AGGTCCTGGC TGAGGTGGCA 
GGCATCTTGG AACCTGTAGG CCTGCAGGAG GAGGCAGAAC TGCCAGCCAA GATCCTGGTT 
GAGTTTGTGG TGGACTCTCA GAAGAAAGAC AAGCTGCTCT GCAGCCAGCT TCAGGTAGCG 
GATTTCCTGC AGAACATCCT GGCTCAGGAG GACACTGCTA AGGGTCTCGA CCCCTTGGCT 
TCTGAAGACA CGAGCCGACA GAAGGCAATT GCAGCTAAGG AACAATGGAA AGAGCTGAAG 
GCCACCTACA GGGAGCACGT AGAGGCCATC AAAATTGGCC TCACCAAGGC CCTGACTCAG 
ATGGAGGAAG CCCAGAGGAA ACGGACACAA CTCCGGGAAG CCTTTGAGCA GCTCCAGGCC 
AAGAAACAAA TGGCCATGGA GAAACGCAGA GCAGTCCAGA ACCAGTGGCA GCTACAACAG 
GAGAAGCATC TGCAGCATCT GGCGGAGGTT TCTGCAGAGG TGAGGGAGCG TAAGACAGGG 
ACTCAGCAGG AGCTTGACAG GGTGTTTCAG AAACTTGGAA ACCTGAAGCA GCAGGCAGAA 
CAGGAGCGGG ACAAGCTGCA GAGGTATCAG ACCTTCCTCC AGCTTCTGTA TACCCTGCAG 
GGTAAGCTGT TGTTCCCTGA GGCTGAGGCT GAGGCAGAGA ATCTTCCAGA TGATAAACCC 
CAGCAGCCGA CTCGACCCCA GGAGCAGAGT ACAGGAGACA CCATGGGGAG AGACCCTGGT 
GTGTCCTTCA AGGTAAATGA GAGATGGAAC AGGTGGGCAT GGGCTGGCAG GTGGAGACAG 
AAGCTGGGAG ATTGGCCTTA CCCACTGTCT ATACTGTATT TCTCCAAGGC TGTTGGTCTA 
CAACCTGCTG GAGATGTAAA TTTGCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZWINT ( XM_017015605.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016871094.1 Homo sapiens ZW10 interacting kinetochore protein (ZWINT), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu91045
Clone ID Related Accession (Same CDS sequence)	XM_017015605.1 , XM_017015605.2
Accession Version	XM_017015605.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 786bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	ZW10 interactor isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCAG CGGAGACAGA GGCGGAAGCT GCAGCCCTAG AGGTCCTGGC TGAGGTGGCA 
GGCATCTTGG AACCTGTAGG CCTGCAGGAG GAGGCAGAAC TGCCAGCCAA GATCCTGGTT 
GAGTTTGTGG TGGACTCTCA GAAGAAAGAC AAGCTGCTCT GCAGCCAGCT TCAGGTAGCG 
GATTTCCTGC AGAACATCCT GGCTCAGGAG GACACTGCTA AGGGTCTCGA CCCCTTGGCT 
TCTGAAGACA CGAGCCGACA GAAGGCAATT GCAGCTAAGG AACAATGGAA AGAGCTGAAG 
GCCACCTACA GGGAGCACGT AGAGGCCATC AAAATTGGCC TCACCAAGGC CCTGACTCAG 
ATGGAGGAAG CCCAGAGGAA ACGGACACAA CTCCGGGAAG CCTTTGAGCA GCTCCAGGCC 
AAGAAACAAA TGGCCATGGA GAAACGCAGA GCAGTCCAGA ACCAGTGGCA GCTACAACAG 
GAGAAGCATC TGCAGCATCT GGCGGAGGTT TCTGCAGAGG TGAGGGAGCG TAAGACAGGG 
ACTCAGCAGG AGCTTGACAG GGTGTTTCAG AAACTTGGAA ACCTGAAGCA GCAGGCAGAA 
CAGGAGCGGG ACAAGCTGCA GAGGGTAAGC TGTTGTTCCC TGAGGCTGAG GCTGAGGCAG 
AGAATCTTCC AGATGATAAA CCCCAGCAGC CGACTCGACC CCAGGAGCAG AGTACAGGAG 
ACACCATGGG GAGAGACCCT GGTGTGTCCT TCAAGGCTGT TGGTCTACAA CCTGCTGGAG 
ATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016871094.1
CDS	67..852
Translation	MEAAETEAEAAALEVLAEVAGILEPVGLQEEAELPAKILVEFVVDSQKKDKLLCSQLQVADFLQNILAQEDTAKGLDPLASEDTSRQKAIAAKEQWKELKATYREHVEAIKIGLTKALTQMEEAQRKRTQLREAFEQLQAKKQMAMEKRRAVQNQWQLQQEKHLQHLAEVSAEVRERKTGTQQELDRVFQKLGNLKQQAEQERDKLQRVSCCSLRLRLRQRIFQMINPSSRLDPRSRVQETPWGETLVCPSRLLVYNLLEM

Target ORF information:

RefSeq Version	XM_017015605.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ZW10 interacting kinetochore protein (ZWINT), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017015605.1

ORF Insert Sequence:

ATGGAGGCAG CGGAGACAGA GGCGGAAGCT GCAGCCCTAG AGGTCCTGGC TGAGGTGGCA 
GGCATCTTGG AACCTGTAGG CCTGCAGGAG GAGGCAGAAC TGCCAGCCAA GATCCTGGTT 
GAGTTTGTGG TGGACTCTCA GAAGAAAGAC AAGCTGCTCT GCAGCCAGCT TCAGGTAGCG 
GATTTCCTGC AGAACATCCT GGCTCAGGAG GACACTGCTA AGGGTCTCGA CCCCTTGGCT 
TCTGAAGACA CGAGCCGACA GAAGGCAATT GCAGCTAAGG AACAATGGAA AGAGCTGAAG 
GCCACCTACA GGGAGCACGT AGAGGCCATC AAAATTGGCC TCACCAAGGC CCTGACTCAG 
ATGGAGGAAG CCCAGAGGAA ACGGACACAA CTCCGGGAAG CCTTTGAGCA GCTCCAGGCC 
AAGAAACAAA TGGCCATGGA GAAACGCAGA GCAGTCCAGA ACCAGTGGCA GCTACAACAG 
GAGAAGCATC TGCAGCATCT GGCGGAGGTT TCTGCAGAGG TGAGGGAGCG TAAGACAGGG 
ACTCAGCAGG AGCTTGACAG GGTGTTTCAG AAACTTGGAA ACCTGAAGCA GCAGGCAGAA 
CAGGAGCGGG ACAAGCTGCA GAGGGTAAGC TGTTGTTCCC TGAGGCTGAG GCTGAGGCAG 
AGAATCTTCC AGATGATAAA CCCCAGCAGC CGACTCGACC CCAGGAGCAG AGTACAGGAG 
ACACCATGGG GAGAGACCCT GGTGTGTCCT TCAAGGCTGT TGGTCTACAA CCTGCTGGAG 
ATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZWINT ( XM_017015605.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016871094.1 Homo sapiens ZW10 interacting kinetochore protein (ZWINT), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu91045
Clone ID Related Accession (Same CDS sequence)	XM_017015605.1 , XM_017015605.2
Accession Version	XM_017015605.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 786bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ZW10 interactor isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017015605.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCAG CGGAGACAGA GGCGGAAGCT GCAGCCCTAG AGGTCCTGGC TGAGGTGGCA 
GGCATCTTGG AACCTGTAGG CCTGCAGGAG GAGGCAGAAC TGCCAGCCAA GATCCTGGTT 
GAGTTTGTGG TGGACTCTCA GAAGAAAGAC AAGCTGCTCT GCAGCCAGCT TCAGGTAGCG 
GATTTCCTGC AGAACATCCT GGCTCAGGAG GACACTGCTA AGGGTCTCGA CCCCTTGGCT 
TCTGAAGACA CGAGCCGACA GAAGGCAATT GCAGCTAAGG AACAATGGAA AGAGCTGAAG 
GCCACCTACA GGGAGCACGT AGAGGCCATC AAAATTGGCC TCACCAAGGC CCTGACTCAG 
ATGGAGGAAG CCCAGAGGAA ACGGACACAA CTCCGGGAAG CCTTTGAGCA GCTCCAGGCC 
AAGAAACAAA TGGCCATGGA GAAACGCAGA GCAGTCCAGA ACCAGTGGCA GCTACAACAG 
GAGAAGCATC TGCAGCATCT GGCGGAGGTT TCTGCAGAGG TGAGGGAGCG TAAGACAGGG 
ACTCAGCAGG AGCTTGACAG GGTGTTTCAG AAACTTGGAA ACCTGAAGCA GCAGGCAGAA 
CAGGAGCGGG ACAAGCTGCA GAGGGTAAGC TGTTGTTCCC TGAGGCTGAG GCTGAGGCAG 
AGAATCTTCC AGATGATAAA CCCCAGCAGC CGACTCGACC CCAGGAGCAG AGTACAGGAG 
ACACCATGGG GAGAGACCCT GGTGTGTCCT TCAAGGCTGT TGGTCTACAA CCTGCTGGAG 
ATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016871094.1
CDS	37..822
Translation	MEAAETEAEAAALEVLAEVAGILEPVGLQEEAELPAKILVEFVVDSQKKDKLLCSQLQVADFLQNILAQEDTAKGLDPLASEDTSRQKAIAAKEQWKELKATYREHVEAIKIGLTKALTQMEEAQRKRTQLREAFEQLQAKKQMAMEKRRAVQNQWQLQQEKHLQHLAEVSAEVRERKTGTQQELDRVFQKLGNLKQQAEQERDKLQRVSCCSLRLRLRQRIFQMINPSSRLDPRSRVQETPWGETLVCPSRLLVYNLLEM

Target ORF information:

RefSeq Version	XM_017015605.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ZW10 interacting kinetochore protein (ZWINT), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017015605.2

ORF Insert Sequence:

ATGGAGGCAG CGGAGACAGA GGCGGAAGCT GCAGCCCTAG AGGTCCTGGC TGAGGTGGCA 
GGCATCTTGG AACCTGTAGG CCTGCAGGAG GAGGCAGAAC TGCCAGCCAA GATCCTGGTT 
GAGTTTGTGG TGGACTCTCA GAAGAAAGAC AAGCTGCTCT GCAGCCAGCT TCAGGTAGCG 
GATTTCCTGC AGAACATCCT GGCTCAGGAG GACACTGCTA AGGGTCTCGA CCCCTTGGCT 
TCTGAAGACA CGAGCCGACA GAAGGCAATT GCAGCTAAGG AACAATGGAA AGAGCTGAAG 
GCCACCTACA GGGAGCACGT AGAGGCCATC AAAATTGGCC TCACCAAGGC CCTGACTCAG 
ATGGAGGAAG CCCAGAGGAA ACGGACACAA CTCCGGGAAG CCTTTGAGCA GCTCCAGGCC 
AAGAAACAAA TGGCCATGGA GAAACGCAGA GCAGTCCAGA ACCAGTGGCA GCTACAACAG 
GAGAAGCATC TGCAGCATCT GGCGGAGGTT TCTGCAGAGG TGAGGGAGCG TAAGACAGGG 
ACTCAGCAGG AGCTTGACAG GGTGTTTCAG AAACTTGGAA ACCTGAAGCA GCAGGCAGAA 
CAGGAGCGGG ACAAGCTGCA GAGGGTAAGC TGTTGTTCCC TGAGGCTGAG GCTGAGGCAG 
AGAATCTTCC AGATGATAAA CCCCAGCAGC CGACTCGACC CCAGGAGCAG AGTACAGGAG 
ACACCATGGG GAGAGACCCT GGTGTGTCCT TCAAGGCTGT TGGTCTACAA CCTGCTGGAG 
ATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

Zwint-1 is required for spindle assembly checkpoint function and kinetochore-microtubule attachment during oocyte meiosis.
Scientific reports515431(2015 Oct)
Woo Seo D,Yeop You S,Chung WJ,Cho DH,Kim JS,Su Oh J

A human interactome in three quantitative dimensions organized by stoichiometries and abundances.
Cell163(3)712-23(2015 Oct)
Hein MY,Hubner NC,Poser I,Cox J,Nagaraj N,Toyoda Y,Gak IA,Weisswange I,Mansfeld J,Buchholz F,Hyman AA,Mann M

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ZWINT cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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