CHEK1 gene cDNA ORF clone, Homo sapiens(human)

mRNA	Protein	Name
XM_011542562.2	XP_011540864.1	serine/threonine-protein kinase Chk1 isoform X3
XM_024448337.1	XP_024304105.1	serine/threonine-protein kinase Chk1 isoform X2
XM_017017146.2	XP_016872635.1	serine/threonine-protein kinase Chk1 isoform X2
NM_001114121.2	NP_001107593.1	serine/threonine-protein kinase Chk1 isoform 1
NM_001114122.2	NP_001107594.1	serine/threonine-protein kinase Chk1 isoform 1
NM_001330428.1	NP_001317357.1	serine/threonine-protein kinase Chk1 isoform 4
NM_001330427.1	NP_001317356.1	serine/threonine-protein kinase Chk1 isoform 3
XM_011542560.2	XP_011540862.1	serine/threonine-protein kinase Chk1 isoform X1
NM_001244846.1	NP_001231775.1	serine/threonine-protein kinase Chk1 isoform 2
XM_017017146.1	XP_016872635.1	serine/threonine-protein kinase Chk1 isoform X2
NM_001274.5	NP_001265.2	serine/threonine-protein kinase Chk1 isoform 1

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu27012	NM_001114122.2 Latest version!	Homo sapiens checkpoint kinase 1 (CHEK1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu27012	NM_001274.5 Latest version!	Homo sapiens checkpoint kinase 1 (CHEK1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu27012	NM_001114121.2 Latest version!	Homo sapiens checkpoint kinase 1 (CHEK1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu56853	NM_001330427.1 Latest version!	Homo sapiens checkpoint kinase 1 (CHEK1), transcript variant 7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu27012	XM_024448337.1 Latest version!	Homo sapiens checkpoint kinase 1 (CHEK1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu27012	XM_017017146.2 Latest version!	Homo sapiens checkpoint kinase 1 (CHEK1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu56853	XM_011542560.2 Latest version!	Homo sapiens checkpoint kinase 1 (CHEK1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu27174	NM_001244846.1 Latest version!	Homo sapiens checkpoint kinase 1 (CHEK1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu56855	NM_001330428.1 Latest version!	Homo sapiens checkpoint kinase 1 (CHEK1), transcript variant 8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu56854	XM_011542562.2 Latest version!	Homo sapiens checkpoint kinase 1 (CHEK1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
View Old Accession Versions >>
OHu27012	XM_017017146.1	Homo sapiens checkpoint kinase 1 (CHEK1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
Hide Old Accession Versions >>

CHEK1 ( NM_001114121.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001107593.1 Homo sapiens checkpoint kinase 1 (CHEK1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu27012
Clone ID Related Accession (Same CDS sequence)	XM_024448337.1 , XM_017017146.2 , NM_001114121.2 , NM_001114122.2 , XM_017017146.1 , NM_001274.5
Accession Version	NM_001114121.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1431bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase Chk1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX419129.2, AW149126.1, CA868104.1, AK300082.1, DA567616.1, BC004202.2 and CA441277.1. This sequence is a reference standard in the RefSeqGene project. On Oct 4, 2011 this sequence version replaced NM_001114121.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC004202.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142348, SAMEA2148874 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCAGTGC CCTTTGTGGA AGACTGGGAC TTGGTGCAAA CCCTGGGAGA AGGTGCCTAT 
GGAGAAGTTC AACTTGCTGT GAATAGAGTA ACTGAAGAAG CAGTCGCAGT GAAGATTGTA 
GATATGAAGC GTGCCGTAGA CTGTCCAGAA AATATTAAGA AAGAGATCTG TATCAATAAA 
ATGCTAAATC ATGAAAATGT AGTAAAATTC TATGGTCACA GGAGAGAAGG CAATATCCAA 
TATTTATTTC TGGAGTACTG TAGTGGAGGA GAGCTTTTTG ACAGAATAGA GCCAGACATA 
GGCATGCCTG AACCAGATGC TCAGAGATTC TTCCATCAAC TCATGGCAGG GGTGGTTTAT 
CTGCATGGTA TTGGAATAAC TCACAGGGAT ATTAAACCAG AAAATCTTCT GTTGGATGAA 
AGGGATAACC TCAAAATCTC AGACTTTGGC TTGGCAACAG TATTTCGGTA TAATAATCGT 
GAGCGTTTGT TGAACAAGAT GTGTGGTACT TTACCATATG TTGCTCCAGA ACTTCTGAAG 
AGAAGAGAAT TTCATGCAGA ACCAGTTGAT GTTTGGTCCT GTGGAATAGT ACTTACTGCA 
ATGCTCGCTG GAGAATTGCC ATGGGACCAA CCCAGTGACA GCTGTCAGGA GTATTCTGAC 
TGGAAAGAAA AAAAAACATA CCTCAACCCT TGGAAAAAAA TCGATTCTGC TCCTCTAGCT 
CTGCTGCATA AAATCTTAGT TGAGAATCCA TCAGCAAGAA TTACCATTCC AGACATCAAA 
AAAGATAGAT GGTACAACAA ACCCCTCAAG AAAGGGGCAA AAAGGCCCCG AGTCACTTCA 
GGTGGTGTGT CAGAGTCTCC CAGTGGATTT TCTAAGCACA TTCAATCCAA TTTGGACTTC 
TCTCCAGTAA ACAGTGCTTC TAGTGAAGAA AATGTGAAGT ACTCCAGTTC TCAGCCAGAA 
CCCCGCACAG GTCTTTCCTT ATGGGATACC AGCCCCTCAT ACATTGATAA ATTGGTACAA 
GGGATCAGCT TTTCCCAGCC CACATGTCCT GATCATATGC TTTTGAATAG TCAGTTACTT 
GGCACCCCAG GATCCTCACA GAACCCCTGG CAGCGGTTGG TCAAAAGAAT GACACGATTC 
TTTACCAAAT TGGATGCAGA CAAATCTTAT CAATGCCTGA AAGAGACTTG TGAGAAGTTG 
GGCTATCAAT GGAAGAAAAG TTGTATGAAT CAGGTTACTA TATCAACAAC TGATAGGAGA 
AACAATAAAC TCATTTTCAA AGTGAATTTG TTAGAAATGG ATGATAAAAT ATTGGTTGAC 
TTCCGGCTTT CTAAGGGTGA TGGATTGGAG TTCAAGAGAC ACTTCCTGAA GATTAAAGGG 
AAGCTGATTG ATATTGTGAG CAGCCAGAAG ATTTGGCTTC CTGCCACATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001107593.1
CDS	898..2328
Translation	MAVPFVEDWDLVQTLGEGAYGEVQLAVNRVTEEAVAVKIVDMKRAVDCPENIKKEICINKMLNHENVVKFYGHRREGNIQYLFLEYCSGGELFDRIEPDIGMPEPDAQRFFHQLMAGVVYLHGIGITHRDIKPENLLLDERDNLKISDFGLATVFRYNNRERLLNKMCGTLPYVAPELLKRREFHAEPVDVWSCGIVLTAMLAGELPWDQPSDSCQEYSDWKEKKTYLNPWKKIDSAPLALLHKILVENPSARITIPDIKKDRWYNKPLKKGAKRPRVTSGGVSESPSGFSKHIQSNLDFSPVNSASSEENVKYSSSQPEPRTGLSLWDTSPSYIDKLVQGISFSQPTCPDHMLLNSQLLGTPGSSQNPWQRLVKRMTRFFTKLDADKSYQCLKETCEKLGYQWKKSCMNQVTISTTDRRNNKLIFKVNLLEMDDKILVDFRLSKGDGLEFKRHFLKIKGKLIDIVSSQKIWLPAT

Target ORF information:

RefSeq Version	NM_001114121.2
Organism	Homo sapiens(human)
Definition	Homo sapiens checkpoint kinase 1 (CHEK1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001114121.2

ORF Insert Sequence:

ATGGCAGTGC CCTTTGTGGA AGACTGGGAC TTGGTGCAAA CCCTGGGAGA AGGTGCCTAT 
GGAGAAGTTC AACTTGCTGT GAATAGAGTA ACTGAAGAAG CAGTCGCAGT GAAGATTGTA 
GATATGAAGC GTGCCGTAGA CTGTCCAGAA AATATTAAGA AAGAGATCTG TATCAATAAA 
ATGCTAAATC ATGAAAATGT AGTAAAATTC TATGGTCACA GGAGAGAAGG CAATATCCAA 
TATTTATTTC TGGAGTACTG TAGTGGAGGA GAGCTTTTTG ACAGAATAGA GCCAGACATA 
GGCATGCCTG AACCAGATGC TCAGAGATTC TTCCATCAAC TCATGGCAGG GGTGGTTTAT 
CTGCATGGTA TTGGAATAAC TCACAGGGAT ATTAAACCAG AAAATCTTCT GTTGGATGAA 
AGGGATAACC TCAAAATCTC AGACTTTGGC TTGGCAACAG TATTTCGGTA TAATAATCGT 
GAGCGTTTGT TGAACAAGAT GTGTGGTACT TTACCATATG TTGCTCCAGA ACTTCTGAAG 
AGAAGAGAAT TTCATGCAGA ACCAGTTGAT GTTTGGTCCT GTGGAATAGT ACTTACTGCA 
ATGCTCGCTG GAGAATTGCC ATGGGACCAA CCCAGTGACA GCTGTCAGGA GTATTCTGAC 
TGGAAAGAAA AAAAAACATA CCTCAACCCT TGGAAAAAAA TCGATTCTGC TCCTCTAGCT 
CTGCTGCATA AAATCTTAGT TGAGAATCCA TCAGCAAGAA TTACCATTCC AGACATCAAA 
AAAGATAGAT GGTACAACAA ACCCCTCAAG AAAGGGGCAA AAAGGCCCCG AGTCACTTCA 
GGTGGTGTGT CAGAGTCTCC CAGTGGATTT TCTAAGCACA TTCAATCCAA TTTGGACTTC 
TCTCCAGTAA ACAGTGCTTC TAGTGAAGAA AATGTGAAGT ACTCCAGTTC TCAGCCAGAA 
CCCCGCACAG GTCTTTCCTT ATGGGATACC AGCCCCTCAT ACATTGATAA ATTGGTACAA 
GGGATCAGCT TTTCCCAGCC CACATGTCCT GATCATATGC TTTTGAATAG TCAGTTACTT 
GGCACCCCAG GATCCTCACA GAACCCCTGG CAGCGGTTGG TCAAAAGAAT GACACGATTC 
TTTACCAAAT TGGATGCAGA CAAATCTTAT CAATGCCTGA AAGAGACTTG TGAGAAGTTG 
GGCTATCAAT GGAAGAAAAG TTGTATGAAT CAGGTTACTA TATCAACAAC TGATAGGAGA 
AACAATAAAC TCATTTTCAA AGTGAATTTG TTAGAAATGG ATGATAAAAT ATTGGTTGAC 
TTCCGGCTTT CTAAGGGTGA TGGATTGGAG TTCAAGAGAC ACTTCCTGAA GATTAAAGGG 
AAGCTGATTG ATATTGTGAG CAGCCAGAAG ATTTGGCTTC CTGCCACATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHEK1 ( NM_001114122.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001107594.1 Homo sapiens checkpoint kinase 1 (CHEK1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu27012
Clone ID Related Accession (Same CDS sequence)	XM_024448337.1 , XM_017017146.2 , NM_001114121.2 , NM_001114122.2 , XM_017017146.1 , NM_001274.5
Accession Version	NM_001114122.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1431bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-31
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase Chk1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX419129.2, AW149126.1, CA868104.1, AK300082.1, DA567616.1, BC004202.2, CA441277.1, BG191840.1, BM455102.1, BX506561.1, BX114781.1, AI750792.1 and AA224205.1. On Oct 4, 2011 this sequence version replaced NM_001114122.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.203825.1, SRR1163658.431825.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCAGTGC CCTTTGTGGA AGACTGGGAC TTGGTGCAAA CCCTGGGAGA AGGTGCCTAT 
GGAGAAGTTC AACTTGCTGT GAATAGAGTA ACTGAAGAAG CAGTCGCAGT GAAGATTGTA 
GATATGAAGC GTGCCGTAGA CTGTCCAGAA AATATTAAGA AAGAGATCTG TATCAATAAA 
ATGCTAAATC ATGAAAATGT AGTAAAATTC TATGGTCACA GGAGAGAAGG CAATATCCAA 
TATTTATTTC TGGAGTACTG TAGTGGAGGA GAGCTTTTTG ACAGAATAGA GCCAGACATA 
GGCATGCCTG AACCAGATGC TCAGAGATTC TTCCATCAAC TCATGGCAGG GGTGGTTTAT 
CTGCATGGTA TTGGAATAAC TCACAGGGAT ATTAAACCAG AAAATCTTCT GTTGGATGAA 
AGGGATAACC TCAAAATCTC AGACTTTGGC TTGGCAACAG TATTTCGGTA TAATAATCGT 
GAGCGTTTGT TGAACAAGAT GTGTGGTACT TTACCATATG TTGCTCCAGA ACTTCTGAAG 
AGAAGAGAAT TTCATGCAGA ACCAGTTGAT GTTTGGTCCT GTGGAATAGT ACTTACTGCA 
ATGCTCGCTG GAGAATTGCC ATGGGACCAA CCCAGTGACA GCTGTCAGGA GTATTCTGAC 
TGGAAAGAAA AAAAAACATA CCTCAACCCT TGGAAAAAAA TCGATTCTGC TCCTCTAGCT 
CTGCTGCATA AAATCTTAGT TGAGAATCCA TCAGCAAGAA TTACCATTCC AGACATCAAA 
AAAGATAGAT GGTACAACAA ACCCCTCAAG AAAGGGGCAA AAAGGCCCCG AGTCACTTCA 
GGTGGTGTGT CAGAGTCTCC CAGTGGATTT TCTAAGCACA TTCAATCCAA TTTGGACTTC 
TCTCCAGTAA ACAGTGCTTC TAGTGAAGAA AATGTGAAGT ACTCCAGTTC TCAGCCAGAA 
CCCCGCACAG GTCTTTCCTT ATGGGATACC AGCCCCTCAT ACATTGATAA ATTGGTACAA 
GGGATCAGCT TTTCCCAGCC CACATGTCCT GATCATATGC TTTTGAATAG TCAGTTACTT 
GGCACCCCAG GATCCTCACA GAACCCCTGG CAGCGGTTGG TCAAAAGAAT GACACGATTC 
TTTACCAAAT TGGATGCAGA CAAATCTTAT CAATGCCTGA AAGAGACTTG TGAGAAGTTG 
GGCTATCAAT GGAAGAAAAG TTGTATGAAT CAGGTTACTA TATCAACAAC TGATAGGAGA 
AACAATAAAC TCATTTTCAA AGTGAATTTG TTAGAAATGG ATGATAAAAT ATTGGTTGAC 
TTCCGGCTTT CTAAGGGTGA TGGATTGGAG TTCAAGAGAC ACTTCCTGAA GATTAAAGGG 
AAGCTGATTG ATATTGTGAG CAGCCAGAAG ATTTGGCTTC CTGCCACATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001107594.1
CDS	898..2328
Translation	MAVPFVEDWDLVQTLGEGAYGEVQLAVNRVTEEAVAVKIVDMKRAVDCPENIKKEICINKMLNHENVVKFYGHRREGNIQYLFLEYCSGGELFDRIEPDIGMPEPDAQRFFHQLMAGVVYLHGIGITHRDIKPENLLLDERDNLKISDFGLATVFRYNNRERLLNKMCGTLPYVAPELLKRREFHAEPVDVWSCGIVLTAMLAGELPWDQPSDSCQEYSDWKEKKTYLNPWKKIDSAPLALLHKILVENPSARITIPDIKKDRWYNKPLKKGAKRPRVTSGGVSESPSGFSKHIQSNLDFSPVNSASSEENVKYSSSQPEPRTGLSLWDTSPSYIDKLVQGISFSQPTCPDHMLLNSQLLGTPGSSQNPWQRLVKRMTRFFTKLDADKSYQCLKETCEKLGYQWKKSCMNQVTISTTDRRNNKLIFKVNLLEMDDKILVDFRLSKGDGLEFKRHFLKIKGKLIDIVSSQKIWLPAT

Target ORF information:

RefSeq Version	NM_001114122.2
Organism	Homo sapiens(human)
Definition	Homo sapiens checkpoint kinase 1 (CHEK1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001114122.2

ORF Insert Sequence:

ATGGCAGTGC CCTTTGTGGA AGACTGGGAC TTGGTGCAAA CCCTGGGAGA AGGTGCCTAT 
GGAGAAGTTC AACTTGCTGT GAATAGAGTA ACTGAAGAAG CAGTCGCAGT GAAGATTGTA 
GATATGAAGC GTGCCGTAGA CTGTCCAGAA AATATTAAGA AAGAGATCTG TATCAATAAA 
ATGCTAAATC ATGAAAATGT AGTAAAATTC TATGGTCACA GGAGAGAAGG CAATATCCAA 
TATTTATTTC TGGAGTACTG TAGTGGAGGA GAGCTTTTTG ACAGAATAGA GCCAGACATA 
GGCATGCCTG AACCAGATGC TCAGAGATTC TTCCATCAAC TCATGGCAGG GGTGGTTTAT 
CTGCATGGTA TTGGAATAAC TCACAGGGAT ATTAAACCAG AAAATCTTCT GTTGGATGAA 
AGGGATAACC TCAAAATCTC AGACTTTGGC TTGGCAACAG TATTTCGGTA TAATAATCGT 
GAGCGTTTGT TGAACAAGAT GTGTGGTACT TTACCATATG TTGCTCCAGA ACTTCTGAAG 
AGAAGAGAAT TTCATGCAGA ACCAGTTGAT GTTTGGTCCT GTGGAATAGT ACTTACTGCA 
ATGCTCGCTG GAGAATTGCC ATGGGACCAA CCCAGTGACA GCTGTCAGGA GTATTCTGAC 
TGGAAAGAAA AAAAAACATA CCTCAACCCT TGGAAAAAAA TCGATTCTGC TCCTCTAGCT 
CTGCTGCATA AAATCTTAGT TGAGAATCCA TCAGCAAGAA TTACCATTCC AGACATCAAA 
AAAGATAGAT GGTACAACAA ACCCCTCAAG AAAGGGGCAA AAAGGCCCCG AGTCACTTCA 
GGTGGTGTGT CAGAGTCTCC CAGTGGATTT TCTAAGCACA TTCAATCCAA TTTGGACTTC 
TCTCCAGTAA ACAGTGCTTC TAGTGAAGAA AATGTGAAGT ACTCCAGTTC TCAGCCAGAA 
CCCCGCACAG GTCTTTCCTT ATGGGATACC AGCCCCTCAT ACATTGATAA ATTGGTACAA 
GGGATCAGCT TTTCCCAGCC CACATGTCCT GATCATATGC TTTTGAATAG TCAGTTACTT 
GGCACCCCAG GATCCTCACA GAACCCCTGG CAGCGGTTGG TCAAAAGAAT GACACGATTC 
TTTACCAAAT TGGATGCAGA CAAATCTTAT CAATGCCTGA AAGAGACTTG TGAGAAGTTG 
GGCTATCAAT GGAAGAAAAG TTGTATGAAT CAGGTTACTA TATCAACAAC TGATAGGAGA 
AACAATAAAC TCATTTTCAA AGTGAATTTG TTAGAAATGG ATGATAAAAT ATTGGTTGAC 
TTCCGGCTTT CTAAGGGTGA TGGATTGGAG TTCAAGAGAC ACTTCCTGAA GATTAAAGGG 
AAGCTGATTG ATATTGTGAG CAGCCAGAAG ATTTGGCTTC CTGCCACATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHEK1 ( NM_001274.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_001265.2 Homo sapiens checkpoint kinase 1 (CHEK1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu27012
Clone ID Related Accession (Same CDS sequence)	XM_024448337.1 , XM_017017146.2 , NM_001114121.2 , NM_001114122.2 , XM_017017146.1 , NM_001274.5
Accession Version	NM_001274.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1431bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase Chk1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP203333.1, BC017575.1, CA441277.1, BC004202.2, BG191840.1, BM455102.1, BX506561.1, BX114781.1, AI750792.1 and AA224205.1. On Oct 4, 2011 this sequence version replaced NM_001274.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC017575.1, SRR1803615.40036.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCAGTGC CCTTTGTGGA AGACTGGGAC TTGGTGCAAA CCCTGGGAGA AGGTGCCTAT 
GGAGAAGTTC AACTTGCTGT GAATAGAGTA ACTGAAGAAG CAGTCGCAGT GAAGATTGTA 
GATATGAAGC GTGCCGTAGA CTGTCCAGAA AATATTAAGA AAGAGATCTG TATCAATAAA 
ATGCTAAATC ATGAAAATGT AGTAAAATTC TATGGTCACA GGAGAGAAGG CAATATCCAA 
TATTTATTTC TGGAGTACTG TAGTGGAGGA GAGCTTTTTG ACAGAATAGA GCCAGACATA 
GGCATGCCTG AACCAGATGC TCAGAGATTC TTCCATCAAC TCATGGCAGG GGTGGTTTAT 
CTGCATGGTA TTGGAATAAC TCACAGGGAT ATTAAACCAG AAAATCTTCT GTTGGATGAA 
AGGGATAACC TCAAAATCTC AGACTTTGGC TTGGCAACAG TATTTCGGTA TAATAATCGT 
GAGCGTTTGT TGAACAAGAT GTGTGGTACT TTACCATATG TTGCTCCAGA ACTTCTGAAG 
AGAAGAGAAT TTCATGCAGA ACCAGTTGAT GTTTGGTCCT GTGGAATAGT ACTTACTGCA 
ATGCTCGCTG GAGAATTGCC ATGGGACCAA CCCAGTGACA GCTGTCAGGA GTATTCTGAC 
TGGAAAGAAA AAAAAACATA CCTCAACCCT TGGAAAAAAA TCGATTCTGC TCCTCTAGCT 
CTGCTGCATA AAATCTTAGT TGAGAATCCA TCAGCAAGAA TTACCATTCC AGACATCAAA 
AAAGATAGAT GGTACAACAA ACCCCTCAAG AAAGGGGCAA AAAGGCCCCG AGTCACTTCA 
GGTGGTGTGT CAGAGTCTCC CAGTGGATTT TCTAAGCACA TTCAATCCAA TTTGGACTTC 
TCTCCAGTAA ACAGTGCTTC TAGTGAAGAA AATGTGAAGT ACTCCAGTTC TCAGCCAGAA 
CCCCGCACAG GTCTTTCCTT ATGGGATACC AGCCCCTCAT ACATTGATAA ATTGGTACAA 
GGGATCAGCT TTTCCCAGCC CACATGTCCT GATCATATGC TTTTGAATAG TCAGTTACTT 
GGCACCCCAG GATCCTCACA GAACCCCTGG CAGCGGTTGG TCAAAAGAAT GACACGATTC 
TTTACCAAAT TGGATGCAGA CAAATCTTAT CAATGCCTGA AAGAGACTTG TGAGAAGTTG 
GGCTATCAAT GGAAGAAAAG TTGTATGAAT CAGGTTACTA TATCAACAAC TGATAGGAGA 
AACAATAAAC TCATTTTCAA AGTGAATTTG TTAGAAATGG ATGATAAAAT ATTGGTTGAC 
TTCCGGCTTT CTAAGGGTGA TGGATTGGAG TTCAAGAGAC ACTTCCTGAA GATTAAAGGG 
AAGCTGATTG ATATTGTGAG CAGCCAGAAG ATTTGGCTTC CTGCCACATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001265.2
CDS	241..1671
Translation	MAVPFVEDWDLVQTLGEGAYGEVQLAVNRVTEEAVAVKIVDMKRAVDCPENIKKEICINKMLNHENVVKFYGHRREGNIQYLFLEYCSGGELFDRIEPDIGMPEPDAQRFFHQLMAGVVYLHGIGITHRDIKPENLLLDERDNLKISDFGLATVFRYNNRERLLNKMCGTLPYVAPELLKRREFHAEPVDVWSCGIVLTAMLAGELPWDQPSDSCQEYSDWKEKKTYLNPWKKIDSAPLALLHKILVENPSARITIPDIKKDRWYNKPLKKGAKRPRVTSGGVSESPSGFSKHIQSNLDFSPVNSASSEENVKYSSSQPEPRTGLSLWDTSPSYIDKLVQGISFSQPTCPDHMLLNSQLLGTPGSSQNPWQRLVKRMTRFFTKLDADKSYQCLKETCEKLGYQWKKSCMNQVTISTTDRRNNKLIFKVNLLEMDDKILVDFRLSKGDGLEFKRHFLKIKGKLIDIVSSQKIWLPAT

Target ORF information:

RefSeq Version	NM_001274.5
Organism	Homo sapiens(human)
Definition	Homo sapiens checkpoint kinase 1 (CHEK1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274.5

ORF Insert Sequence:

ATGGCAGTGC CCTTTGTGGA AGACTGGGAC TTGGTGCAAA CCCTGGGAGA AGGTGCCTAT 
GGAGAAGTTC AACTTGCTGT GAATAGAGTA ACTGAAGAAG CAGTCGCAGT GAAGATTGTA 
GATATGAAGC GTGCCGTAGA CTGTCCAGAA AATATTAAGA AAGAGATCTG TATCAATAAA 
ATGCTAAATC ATGAAAATGT AGTAAAATTC TATGGTCACA GGAGAGAAGG CAATATCCAA 
TATTTATTTC TGGAGTACTG TAGTGGAGGA GAGCTTTTTG ACAGAATAGA GCCAGACATA 
GGCATGCCTG AACCAGATGC TCAGAGATTC TTCCATCAAC TCATGGCAGG GGTGGTTTAT 
CTGCATGGTA TTGGAATAAC TCACAGGGAT ATTAAACCAG AAAATCTTCT GTTGGATGAA 
AGGGATAACC TCAAAATCTC AGACTTTGGC TTGGCAACAG TATTTCGGTA TAATAATCGT 
GAGCGTTTGT TGAACAAGAT GTGTGGTACT TTACCATATG TTGCTCCAGA ACTTCTGAAG 
AGAAGAGAAT TTCATGCAGA ACCAGTTGAT GTTTGGTCCT GTGGAATAGT ACTTACTGCA 
ATGCTCGCTG GAGAATTGCC ATGGGACCAA CCCAGTGACA GCTGTCAGGA GTATTCTGAC 
TGGAAAGAAA AAAAAACATA CCTCAACCCT TGGAAAAAAA TCGATTCTGC TCCTCTAGCT 
CTGCTGCATA AAATCTTAGT TGAGAATCCA TCAGCAAGAA TTACCATTCC AGACATCAAA 
AAAGATAGAT GGTACAACAA ACCCCTCAAG AAAGGGGCAA AAAGGCCCCG AGTCACTTCA 
GGTGGTGTGT CAGAGTCTCC CAGTGGATTT TCTAAGCACA TTCAATCCAA TTTGGACTTC 
TCTCCAGTAA ACAGTGCTTC TAGTGAAGAA AATGTGAAGT ACTCCAGTTC TCAGCCAGAA 
CCCCGCACAG GTCTTTCCTT ATGGGATACC AGCCCCTCAT ACATTGATAA ATTGGTACAA 
GGGATCAGCT TTTCCCAGCC CACATGTCCT GATCATATGC TTTTGAATAG TCAGTTACTT 
GGCACCCCAG GATCCTCACA GAACCCCTGG CAGCGGTTGG TCAAAAGAAT GACACGATTC 
TTTACCAAAT TGGATGCAGA CAAATCTTAT CAATGCCTGA AAGAGACTTG TGAGAAGTTG 
GGCTATCAAT GGAAGAAAAG TTGTATGAAT CAGGTTACTA TATCAACAAC TGATAGGAGA 
AACAATAAAC TCATTTTCAA AGTGAATTTG TTAGAAATGG ATGATAAAAT ATTGGTTGAC 
TTCCGGCTTT CTAAGGGTGA TGGATTGGAG TTCAAGAGAC ACTTCCTGAA GATTAAAGGG 
AAGCTGATTG ATATTGTGAG CAGCCAGAAG ATTTGGCTTC CTGCCACATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHEK1 ( NM_001330427.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001317356.1 Homo sapiens checkpoint kinase 1 (CHEK1), transcript variant 7, mRNA.

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CloneID	OHu56853
Clone ID Related Accession (Same CDS sequence)	NM_001330427.1 , XM_011542560.2
Accession Version	NM_001330427.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1479bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase Chk1 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AP001132.4. On Aug 29, 2016 this sequence version replaced XM_011542561.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163658.281121.1, SRR1163657.280015.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142348, SAMEA2148874 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCGGTCCG CCGTCCTTAA ATCTCTTCAG CCAGGATCTC TCCCCGACTG CAAAGCAGCC 
CTGGGCGGGA GCGGCAACAT CTCCACGTCA CCCTTTTGGA GCCGCCGACA TTCAGAGGGG 
CAGGACACGG GAACGCGCGC TGTCTTGCTT TACGGCGCGG GTGCGCGAGT TTGCGGCAGC 
GTGACGCCCT CAAGTTTTGG CGGGAAAAGC GCTGCATTTG GATTCCTGCA GTGGTGGGCA 
AAGGACAGTC CGCCGAGGTG CTCGGTGGAG TCATGGCAGT GCCCTTTGTG GAAGACTGGG 
ACTTGGTGCA AACCCTGGGA GAAGGTGCCT ATGGAGAAGC CAGACATAGG CATGCCTGAA 
CCAGATGCTC AGAGATTCTT CCATCAACTC ATGGCAGGGG TGGTTTATCT GCATGGTATT 
GGAATAACTC ACAGGGATAT TAAACCAGAA AATCTTCTGT TGGATGAAAG GGATAACCTC 
AAAATCTCAG ACTTTGGCTT GGCAACAGTA TTTCGGTATA ATAATCGTGA GCGTTTGTTG 
AACAAGATGT GTGGTACTTT ACCATATGTT GCTCCAGAAC TTCTGAAGAG AAGAGAATTT 
CATGCAGAAC CAGTTGATGT TTGGTCCTGT GGAATAGTAC TTACTGCAAT GCTCGCTGGA 
GAATTGCCAT GGGACCAACC CAGTGACAGC TGTCAGGAGT ATTCTGACTG GAAAGAAAAA 
AAAACATACC TCAACCCTTG GAAAAAAATC GATTCTGCTC CTCTAGCTCT GCTGCATAAA 
ATCTTAGTTG AGAATCCATC AGCAAGAATT ACCATTCCAG ACATCAAAAA AGATAGATGG 
TACAACAAAC CCCTCAAGAA AGGGGCAAAA AGGCCCCGAG TCACTTCAGG TGGTGTGTCA 
GAGTCTCCCA GTGGATTTTC TAAGCACATT CAATCCAATT TGGACTTCTC TCCAGTAAAC 
AGTGCTTCTA GTGAAGAAAA TGTGAAGTAC TCCAGTTCTC AGCCAGAACC CCGCACAGGT 
CTTTCCTTAT GGGATACCAG CCCCTCATAC ATTGATAAAT TGGTACAAGG GATCAGCTTT 
TCCCAGCCCA CATGTCCTGA TCATATGCTT TTGAATAGTC AGTTACTTGG CACCCCAGGA 
TCCTCACAGA ACCCCTGGCA GCGGTTGGTC AAAAGAATGA CACGATTCTT TACCAAATTG 
GATGCAGACA AATCTTATCA ATGCCTGAAA GAGACTTGTG AGAAGTTGGG CTATCAATGG 
AAGAAAAGTT GTATGAATCA GGTTACTATA TCAACAACTG ATAGGAGAAA CAATAAACTC 
ATTTTCAAAG TGAATTTGTT AGAAATGGAT GATAAAATAT TGGTTGACTT CCGGCTTTCT 
AAGGGTGATG GATTGGAGTT CAAGAGACAC TTCCTGAAGA TTAAAGGGAA GCTGATTGAT 
ATTGTGAGCA GCCAGAAGAT TTGGCTTCCT GCCACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001317356.1
CDS	338..1816
Translation	MRSAVLKSLQPGSLPDCKAALGGSGNISTSPFWSRRHSEGQDTGTRAVLLYGAGARVCGSVTPSSFGGKSAAFGFLQWWAKDSPPRCSVESWQCPLWKTGTWCKPWEKVPMEKPDIGMPEPDAQRFFHQLMAGVVYLHGIGITHRDIKPENLLLDERDNLKISDFGLATVFRYNNRERLLNKMCGTLPYVAPELLKRREFHAEPVDVWSCGIVLTAMLAGELPWDQPSDSCQEYSDWKEKKTYLNPWKKIDSAPLALLHKILVENPSARITIPDIKKDRWYNKPLKKGAKRPRVTSGGVSESPSGFSKHIQSNLDFSPVNSASSEENVKYSSSQPEPRTGLSLWDTSPSYIDKLVQGISFSQPTCPDHMLLNSQLLGTPGSSQNPWQRLVKRMTRFFTKLDADKSYQCLKETCEKLGYQWKKSCMNQVTISTTDRRNNKLIFKVNLLEMDDKILVDFRLSKGDGLEFKRHFLKIKGKLIDIVSSQKIWLPAT

Target ORF information:

RefSeq Version	NM_001330427.1
Organism	Homo sapiens(human)
Definition	Homo sapiens checkpoint kinase 1 (CHEK1), transcript variant 7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001330427.1

ORF Insert Sequence:

ATGCGGTCCG CCGTCCTTAA ATCTCTTCAG CCAGGATCTC TCCCCGACTG CAAAGCAGCC 
CTGGGCGGGA GCGGCAACAT CTCCACGTCA CCCTTTTGGA GCCGCCGACA TTCAGAGGGG 
CAGGACACGG GAACGCGCGC TGTCTTGCTT TACGGCGCGG GTGCGCGAGT TTGCGGCAGC 
GTGACGCCCT CAAGTTTTGG CGGGAAAAGC GCTGCATTTG GATTCCTGCA GTGGTGGGCA 
AAGGACAGTC CGCCGAGGTG CTCGGTGGAG TCATGGCAGT GCCCTTTGTG GAAGACTGGG 
ACTTGGTGCA AACCCTGGGA GAAGGTGCCT ATGGAGAAGC CAGACATAGG CATGCCTGAA 
CCAGATGCTC AGAGATTCTT CCATCAACTC ATGGCAGGGG TGGTTTATCT GCATGGTATT 
GGAATAACTC ACAGGGATAT TAAACCAGAA AATCTTCTGT TGGATGAAAG GGATAACCTC 
AAAATCTCAG ACTTTGGCTT GGCAACAGTA TTTCGGTATA ATAATCGTGA GCGTTTGTTG 
AACAAGATGT GTGGTACTTT ACCATATGTT GCTCCAGAAC TTCTGAAGAG AAGAGAATTT 
CATGCAGAAC CAGTTGATGT TTGGTCCTGT GGAATAGTAC TTACTGCAAT GCTCGCTGGA 
GAATTGCCAT GGGACCAACC CAGTGACAGC TGTCAGGAGT ATTCTGACTG GAAAGAAAAA 
AAAACATACC TCAACCCTTG GAAAAAAATC GATTCTGCTC CTCTAGCTCT GCTGCATAAA 
ATCTTAGTTG AGAATCCATC AGCAAGAATT ACCATTCCAG ACATCAAAAA AGATAGATGG 
TACAACAAAC CCCTCAAGAA AGGGGCAAAA AGGCCCCGAG TCACTTCAGG TGGTGTGTCA 
GAGTCTCCCA GTGGATTTTC TAAGCACATT CAATCCAATT TGGACTTCTC TCCAGTAAAC 
AGTGCTTCTA GTGAAGAAAA TGTGAAGTAC TCCAGTTCTC AGCCAGAACC CCGCACAGGT 
CTTTCCTTAT GGGATACCAG CCCCTCATAC ATTGATAAAT TGGTACAAGG GATCAGCTTT 
TCCCAGCCCA CATGTCCTGA TCATATGCTT TTGAATAGTC AGTTACTTGG CACCCCAGGA 
TCCTCACAGA ACCCCTGGCA GCGGTTGGTC AAAAGAATGA CACGATTCTT TACCAAATTG 
GATGCAGACA AATCTTATCA ATGCCTGAAA GAGACTTGTG AGAAGTTGGG CTATCAATGG 
AAGAAAAGTT GTATGAATCA GGTTACTATA TCAACAACTG ATAGGAGAAA CAATAAACTC 
ATTTTCAAAG TGAATTTGTT AGAAATGGAT GATAAAATAT TGGTTGACTT CCGGCTTTCT 
AAGGGTGATG GATTGGAGTT CAAGAGACAC TTCCTGAAGA TTAAAGGGAA GCTGATTGAT 
ATTGTGAGCA GCCAGAAGAT TTGGCTTCCT GCCACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHEK1 ( XM_024448337.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024304105.1 Homo sapiens checkpoint kinase 1 (CHEK1), transcript variant X2, mRNA.

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CloneID	OHu27012
Clone ID Related Accession (Same CDS sequence)	XM_024448337.1 , XM_017017146.2 , NM_001114121.2 , NM_001114122.2 , XM_017017146.1 , NM_001274.5
Accession Version	XM_024448337.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1431bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase Chk1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGTGC CCTTTGTGGA AGACTGGGAC TTGGTGCAAA CCCTGGGAGA AGGTGCCTAT 
GGAGAAGTTC AACTTGCTGT GAATAGAGTA ACTGAAGAAG CAGTCGCAGT GAAGATTGTA 
GATATGAAGC GTGCCGTAGA CTGTCCAGAA AATATTAAGA AAGAGATCTG TATCAATAAA 
ATGCTAAATC ATGAAAATGT AGTAAAATTC TATGGTCACA GGAGAGAAGG CAATATCCAA 
TATTTATTTC TGGAGTACTG TAGTGGAGGA GAGCTTTTTG ACAGAATAGA GCCAGACATA 
GGCATGCCTG AACCAGATGC TCAGAGATTC TTCCATCAAC TCATGGCAGG GGTGGTTTAT 
CTGCATGGTA TTGGAATAAC TCACAGGGAT ATTAAACCAG AAAATCTTCT GTTGGATGAA 
AGGGATAACC TCAAAATCTC AGACTTTGGC TTGGCAACAG TATTTCGGTA TAATAATCGT 
GAGCGTTTGT TGAACAAGAT GTGTGGTACT TTACCATATG TTGCTCCAGA ACTTCTGAAG 
AGAAGAGAAT TTCATGCAGA ACCAGTTGAT GTTTGGTCCT GTGGAATAGT ACTTACTGCA 
ATGCTCGCTG GAGAATTGCC ATGGGACCAA CCCAGTGACA GCTGTCAGGA GTATTCTGAC 
TGGAAAGAAA AAAAAACATA CCTCAACCCT TGGAAAAAAA TCGATTCTGC TCCTCTAGCT 
CTGCTGCATA AAATCTTAGT TGAGAATCCA TCAGCAAGAA TTACCATTCC AGACATCAAA 
AAAGATAGAT GGTACAACAA ACCCCTCAAG AAAGGGGCAA AAAGGCCCCG AGTCACTTCA 
GGTGGTGTGT CAGAGTCTCC CAGTGGATTT TCTAAGCACA TTCAATCCAA TTTGGACTTC 
TCTCCAGTAA ACAGTGCTTC TAGTGAAGAA AATGTGAAGT ACTCCAGTTC TCAGCCAGAA 
CCCCGCACAG GTCTTTCCTT ATGGGATACC AGCCCCTCAT ACATTGATAA ATTGGTACAA 
GGGATCAGCT TTTCCCAGCC CACATGTCCT GATCATATGC TTTTGAATAG TCAGTTACTT 
GGCACCCCAG GATCCTCACA GAACCCCTGG CAGCGGTTGG TCAAAAGAAT GACACGATTC 
TTTACCAAAT TGGATGCAGA CAAATCTTAT CAATGCCTGA AAGAGACTTG TGAGAAGTTG 
GGCTATCAAT GGAAGAAAAG TTGTATGAAT CAGGTTACTA TATCAACAAC TGATAGGAGA 
AACAATAAAC TCATTTTCAA AGTGAATTTG TTAGAAATGG ATGATAAAAT ATTGGTTGAC 
TTCCGGCTTT CTAAGGGTGA TGGATTGGAG TTCAAGAGAC ACTTCCTGAA GATTAAAGGG 
AAGCTGATTG ATATTGTGAG CAGCCAGAAG ATTTGGCTTC CTGCCACATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024304105.1
CDS	332..1762
Translation	MAVPFVEDWDLVQTLGEGAYGEVQLAVNRVTEEAVAVKIVDMKRAVDCPENIKKEICINKMLNHENVVKFYGHRREGNIQYLFLEYCSGGELFDRIEPDIGMPEPDAQRFFHQLMAGVVYLHGIGITHRDIKPENLLLDERDNLKISDFGLATVFRYNNRERLLNKMCGTLPYVAPELLKRREFHAEPVDVWSCGIVLTAMLAGELPWDQPSDSCQEYSDWKEKKTYLNPWKKIDSAPLALLHKILVENPSARITIPDIKKDRWYNKPLKKGAKRPRVTSGGVSESPSGFSKHIQSNLDFSPVNSASSEENVKYSSSQPEPRTGLSLWDTSPSYIDKLVQGISFSQPTCPDHMLLNSQLLGTPGSSQNPWQRLVKRMTRFFTKLDADKSYQCLKETCEKLGYQWKKSCMNQVTISTTDRRNNKLIFKVNLLEMDDKILVDFRLSKGDGLEFKRHFLKIKGKLIDIVSSQKIWLPAT

Target ORF information:

RefSeq Version	XM_024448337.1
Organism	Homo sapiens(human)
Definition	Homo sapiens checkpoint kinase 1 (CHEK1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024448337.1

ORF Insert Sequence:

ATGGCAGTGC CCTTTGTGGA AGACTGGGAC TTGGTGCAAA CCCTGGGAGA AGGTGCCTAT 
GGAGAAGTTC AACTTGCTGT GAATAGAGTA ACTGAAGAAG CAGTCGCAGT GAAGATTGTA 
GATATGAAGC GTGCCGTAGA CTGTCCAGAA AATATTAAGA AAGAGATCTG TATCAATAAA 
ATGCTAAATC ATGAAAATGT AGTAAAATTC TATGGTCACA GGAGAGAAGG CAATATCCAA 
TATTTATTTC TGGAGTACTG TAGTGGAGGA GAGCTTTTTG ACAGAATAGA GCCAGACATA 
GGCATGCCTG AACCAGATGC TCAGAGATTC TTCCATCAAC TCATGGCAGG GGTGGTTTAT 
CTGCATGGTA TTGGAATAAC TCACAGGGAT ATTAAACCAG AAAATCTTCT GTTGGATGAA 
AGGGATAACC TCAAAATCTC AGACTTTGGC TTGGCAACAG TATTTCGGTA TAATAATCGT 
GAGCGTTTGT TGAACAAGAT GTGTGGTACT TTACCATATG TTGCTCCAGA ACTTCTGAAG 
AGAAGAGAAT TTCATGCAGA ACCAGTTGAT GTTTGGTCCT GTGGAATAGT ACTTACTGCA 
ATGCTCGCTG GAGAATTGCC ATGGGACCAA CCCAGTGACA GCTGTCAGGA GTATTCTGAC 
TGGAAAGAAA AAAAAACATA CCTCAACCCT TGGAAAAAAA TCGATTCTGC TCCTCTAGCT 
CTGCTGCATA AAATCTTAGT TGAGAATCCA TCAGCAAGAA TTACCATTCC AGACATCAAA 
AAAGATAGAT GGTACAACAA ACCCCTCAAG AAAGGGGCAA AAAGGCCCCG AGTCACTTCA 
GGTGGTGTGT CAGAGTCTCC CAGTGGATTT TCTAAGCACA TTCAATCCAA TTTGGACTTC 
TCTCCAGTAA ACAGTGCTTC TAGTGAAGAA AATGTGAAGT ACTCCAGTTC TCAGCCAGAA 
CCCCGCACAG GTCTTTCCTT ATGGGATACC AGCCCCTCAT ACATTGATAA ATTGGTACAA 
GGGATCAGCT TTTCCCAGCC CACATGTCCT GATCATATGC TTTTGAATAG TCAGTTACTT 
GGCACCCCAG GATCCTCACA GAACCCCTGG CAGCGGTTGG TCAAAAGAAT GACACGATTC 
TTTACCAAAT TGGATGCAGA CAAATCTTAT CAATGCCTGA AAGAGACTTG TGAGAAGTTG 
GGCTATCAAT GGAAGAAAAG TTGTATGAAT CAGGTTACTA TATCAACAAC TGATAGGAGA 
AACAATAAAC TCATTTTCAA AGTGAATTTG TTAGAAATGG ATGATAAAAT ATTGGTTGAC 
TTCCGGCTTT CTAAGGGTGA TGGATTGGAG TTCAAGAGAC ACTTCCTGAA GATTAAAGGG 
AAGCTGATTG ATATTGTGAG CAGCCAGAAG ATTTGGCTTC CTGCCACATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHEK1 ( XM_017017146.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016872635.1 Homo sapiens checkpoint kinase 1 (CHEK1), transcript variant X3, mRNA.

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CloneID	OHu27012
Clone ID Related Accession (Same CDS sequence)	XM_024448337.1 , XM_017017146.2 , NM_001114121.2 , NM_001114122.2 , XM_017017146.1 , NM_001274.5
Accession Version	XM_017017146.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1431bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase Chk1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017017146.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGTGC CCTTTGTGGA AGACTGGGAC TTGGTGCAAA CCCTGGGAGA AGGTGCCTAT 
GGAGAAGTTC AACTTGCTGT GAATAGAGTA ACTGAAGAAG CAGTCGCAGT GAAGATTGTA 
GATATGAAGC GTGCCGTAGA CTGTCCAGAA AATATTAAGA AAGAGATCTG TATCAATAAA 
ATGCTAAATC ATGAAAATGT AGTAAAATTC TATGGTCACA GGAGAGAAGG CAATATCCAA 
TATTTATTTC TGGAGTACTG TAGTGGAGGA GAGCTTTTTG ACAGAATAGA GCCAGACATA 
GGCATGCCTG AACCAGATGC TCAGAGATTC TTCCATCAAC TCATGGCAGG GGTGGTTTAT 
CTGCATGGTA TTGGAATAAC TCACAGGGAT ATTAAACCAG AAAATCTTCT GTTGGATGAA 
AGGGATAACC TCAAAATCTC AGACTTTGGC TTGGCAACAG TATTTCGGTA TAATAATCGT 
GAGCGTTTGT TGAACAAGAT GTGTGGTACT TTACCATATG TTGCTCCAGA ACTTCTGAAG 
AGAAGAGAAT TTCATGCAGA ACCAGTTGAT GTTTGGTCCT GTGGAATAGT ACTTACTGCA 
ATGCTCGCTG GAGAATTGCC ATGGGACCAA CCCAGTGACA GCTGTCAGGA GTATTCTGAC 
TGGAAAGAAA AAAAAACATA CCTCAACCCT TGGAAAAAAA TCGATTCTGC TCCTCTAGCT 
CTGCTGCATA AAATCTTAGT TGAGAATCCA TCAGCAAGAA TTACCATTCC AGACATCAAA 
AAAGATAGAT GGTACAACAA ACCCCTCAAG AAAGGGGCAA AAAGGCCCCG AGTCACTTCA 
GGTGGTGTGT CAGAGTCTCC CAGTGGATTT TCTAAGCACA TTCAATCCAA TTTGGACTTC 
TCTCCAGTAA ACAGTGCTTC TAGTGAAGAA AATGTGAAGT ACTCCAGTTC TCAGCCAGAA 
CCCCGCACAG GTCTTTCCTT ATGGGATACC AGCCCCTCAT ACATTGATAA ATTGGTACAA 
GGGATCAGCT TTTCCCAGCC CACATGTCCT GATCATATGC TTTTGAATAG TCAGTTACTT 
GGCACCCCAG GATCCTCACA GAACCCCTGG CAGCGGTTGG TCAAAAGAAT GACACGATTC 
TTTACCAAAT TGGATGCAGA CAAATCTTAT CAATGCCTGA AAGAGACTTG TGAGAAGTTG 
GGCTATCAAT GGAAGAAAAG TTGTATGAAT CAGGTTACTA TATCAACAAC TGATAGGAGA 
AACAATAAAC TCATTTTCAA AGTGAATTTG TTAGAAATGG ATGATAAAAT ATTGGTTGAC 
TTCCGGCTTT CTAAGGGTGA TGGATTGGAG TTCAAGAGAC ACTTCCTGAA GATTAAAGGG 
AAGCTGATTG ATATTGTGAG CAGCCAGAAG ATTTGGCTTC CTGCCACATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016872635.1
CDS	82..1512
Translation	MAVPFVEDWDLVQTLGEGAYGEVQLAVNRVTEEAVAVKIVDMKRAVDCPENIKKEICINKMLNHENVVKFYGHRREGNIQYLFLEYCSGGELFDRIEPDIGMPEPDAQRFFHQLMAGVVYLHGIGITHRDIKPENLLLDERDNLKISDFGLATVFRYNNRERLLNKMCGTLPYVAPELLKRREFHAEPVDVWSCGIVLTAMLAGELPWDQPSDSCQEYSDWKEKKTYLNPWKKIDSAPLALLHKILVENPSARITIPDIKKDRWYNKPLKKGAKRPRVTSGGVSESPSGFSKHIQSNLDFSPVNSASSEENVKYSSSQPEPRTGLSLWDTSPSYIDKLVQGISFSQPTCPDHMLLNSQLLGTPGSSQNPWQRLVKRMTRFFTKLDADKSYQCLKETCEKLGYQWKKSCMNQVTISTTDRRNNKLIFKVNLLEMDDKILVDFRLSKGDGLEFKRHFLKIKGKLIDIVSSQKIWLPAT

Target ORF information:

RefSeq Version	XM_017017146.2
Organism	Homo sapiens(human)
Definition	Homo sapiens checkpoint kinase 1 (CHEK1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017017146.2

ORF Insert Sequence:

ATGGCAGTGC CCTTTGTGGA AGACTGGGAC TTGGTGCAAA CCCTGGGAGA AGGTGCCTAT 
GGAGAAGTTC AACTTGCTGT GAATAGAGTA ACTGAAGAAG CAGTCGCAGT GAAGATTGTA 
GATATGAAGC GTGCCGTAGA CTGTCCAGAA AATATTAAGA AAGAGATCTG TATCAATAAA 
ATGCTAAATC ATGAAAATGT AGTAAAATTC TATGGTCACA GGAGAGAAGG CAATATCCAA 
TATTTATTTC TGGAGTACTG TAGTGGAGGA GAGCTTTTTG ACAGAATAGA GCCAGACATA 
GGCATGCCTG AACCAGATGC TCAGAGATTC TTCCATCAAC TCATGGCAGG GGTGGTTTAT 
CTGCATGGTA TTGGAATAAC TCACAGGGAT ATTAAACCAG AAAATCTTCT GTTGGATGAA 
AGGGATAACC TCAAAATCTC AGACTTTGGC TTGGCAACAG TATTTCGGTA TAATAATCGT 
GAGCGTTTGT TGAACAAGAT GTGTGGTACT TTACCATATG TTGCTCCAGA ACTTCTGAAG 
AGAAGAGAAT TTCATGCAGA ACCAGTTGAT GTTTGGTCCT GTGGAATAGT ACTTACTGCA 
ATGCTCGCTG GAGAATTGCC ATGGGACCAA CCCAGTGACA GCTGTCAGGA GTATTCTGAC 
TGGAAAGAAA AAAAAACATA CCTCAACCCT TGGAAAAAAA TCGATTCTGC TCCTCTAGCT 
CTGCTGCATA AAATCTTAGT TGAGAATCCA TCAGCAAGAA TTACCATTCC AGACATCAAA 
AAAGATAGAT GGTACAACAA ACCCCTCAAG AAAGGGGCAA AAAGGCCCCG AGTCACTTCA 
GGTGGTGTGT CAGAGTCTCC CAGTGGATTT TCTAAGCACA TTCAATCCAA TTTGGACTTC 
TCTCCAGTAA ACAGTGCTTC TAGTGAAGAA AATGTGAAGT ACTCCAGTTC TCAGCCAGAA 
CCCCGCACAG GTCTTTCCTT ATGGGATACC AGCCCCTCAT ACATTGATAA ATTGGTACAA 
GGGATCAGCT TTTCCCAGCC CACATGTCCT GATCATATGC TTTTGAATAG TCAGTTACTT 
GGCACCCCAG GATCCTCACA GAACCCCTGG CAGCGGTTGG TCAAAAGAAT GACACGATTC 
TTTACCAAAT TGGATGCAGA CAAATCTTAT CAATGCCTGA AAGAGACTTG TGAGAAGTTG 
GGCTATCAAT GGAAGAAAAG TTGTATGAAT CAGGTTACTA TATCAACAAC TGATAGGAGA 
AACAATAAAC TCATTTTCAA AGTGAATTTG TTAGAAATGG ATGATAAAAT ATTGGTTGAC 
TTCCGGCTTT CTAAGGGTGA TGGATTGGAG TTCAAGAGAC ACTTCCTGAA GATTAAAGGG 
AAGCTGATTG ATATTGTGAG CAGCCAGAAG ATTTGGCTTC CTGCCACATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHEK1 ( XM_017017146.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016872635.1 Homo sapiens checkpoint kinase 1 (CHEK1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu27012
Clone ID Related Accession (Same CDS sequence)	XM_024448337.1 , XM_017017146.2 , NM_001114121.2 , NM_001114122.2 , XM_017017146.1 , NM_001274.5
Accession Version	XM_017017146.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1431bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase Chk1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGTGC CCTTTGTGGA AGACTGGGAC TTGGTGCAAA CCCTGGGAGA AGGTGCCTAT 
GGAGAAGTTC AACTTGCTGT GAATAGAGTA ACTGAAGAAG CAGTCGCAGT GAAGATTGTA 
GATATGAAGC GTGCCGTAGA CTGTCCAGAA AATATTAAGA AAGAGATCTG TATCAATAAA 
ATGCTAAATC ATGAAAATGT AGTAAAATTC TATGGTCACA GGAGAGAAGG CAATATCCAA 
TATTTATTTC TGGAGTACTG TAGTGGAGGA GAGCTTTTTG ACAGAATAGA GCCAGACATA 
GGCATGCCTG AACCAGATGC TCAGAGATTC TTCCATCAAC TCATGGCAGG GGTGGTTTAT 
CTGCATGGTA TTGGAATAAC TCACAGGGAT ATTAAACCAG AAAATCTTCT GTTGGATGAA 
AGGGATAACC TCAAAATCTC AGACTTTGGC TTGGCAACAG TATTTCGGTA TAATAATCGT 
GAGCGTTTGT TGAACAAGAT GTGTGGTACT TTACCATATG TTGCTCCAGA ACTTCTGAAG 
AGAAGAGAAT TTCATGCAGA ACCAGTTGAT GTTTGGTCCT GTGGAATAGT ACTTACTGCA 
ATGCTCGCTG GAGAATTGCC ATGGGACCAA CCCAGTGACA GCTGTCAGGA GTATTCTGAC 
TGGAAAGAAA AAAAAACATA CCTCAACCCT TGGAAAAAAA TCGATTCTGC TCCTCTAGCT 
CTGCTGCATA AAATCTTAGT TGAGAATCCA TCAGCAAGAA TTACCATTCC AGACATCAAA 
AAAGATAGAT GGTACAACAA ACCCCTCAAG AAAGGGGCAA AAAGGCCCCG AGTCACTTCA 
GGTGGTGTGT CAGAGTCTCC CAGTGGATTT TCTAAGCACA TTCAATCCAA TTTGGACTTC 
TCTCCAGTAA ACAGTGCTTC TAGTGAAGAA AATGTGAAGT ACTCCAGTTC TCAGCCAGAA 
CCCCGCACAG GTCTTTCCTT ATGGGATACC AGCCCCTCAT ACATTGATAA ATTGGTACAA 
GGGATCAGCT TTTCCCAGCC CACATGTCCT GATCATATGC TTTTGAATAG TCAGTTACTT 
GGCACCCCAG GATCCTCACA GAACCCCTGG CAGCGGTTGG TCAAAAGAAT GACACGATTC 
TTTACCAAAT TGGATGCAGA CAAATCTTAT CAATGCCTGA AAGAGACTTG TGAGAAGTTG 
GGCTATCAAT GGAAGAAAAG TTGTATGAAT CAGGTTACTA TATCAACAAC TGATAGGAGA 
AACAATAAAC TCATTTTCAA AGTGAATTTG TTAGAAATGG ATGATAAAAT ATTGGTTGAC 
TTCCGGCTTT CTAAGGGTGA TGGATTGGAG TTCAAGAGAC ACTTCCTGAA GATTAAAGGG 
AAGCTGATTG ATATTGTGAG CAGCCAGAAG ATTTGGCTTC CTGCCACATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016872635.1
CDS	85..1515
Translation	MAVPFVEDWDLVQTLGEGAYGEVQLAVNRVTEEAVAVKIVDMKRAVDCPENIKKEICINKMLNHENVVKFYGHRREGNIQYLFLEYCSGGELFDRIEPDIGMPEPDAQRFFHQLMAGVVYLHGIGITHRDIKPENLLLDERDNLKISDFGLATVFRYNNRERLLNKMCGTLPYVAPELLKRREFHAEPVDVWSCGIVLTAMLAGELPWDQPSDSCQEYSDWKEKKTYLNPWKKIDSAPLALLHKILVENPSARITIPDIKKDRWYNKPLKKGAKRPRVTSGGVSESPSGFSKHIQSNLDFSPVNSASSEENVKYSSSQPEPRTGLSLWDTSPSYIDKLVQGISFSQPTCPDHMLLNSQLLGTPGSSQNPWQRLVKRMTRFFTKLDADKSYQCLKETCEKLGYQWKKSCMNQVTISTTDRRNNKLIFKVNLLEMDDKILVDFRLSKGDGLEFKRHFLKIKGKLIDIVSSQKIWLPAT

Target ORF information:

RefSeq Version	XM_017017146.1
Organism	Homo sapiens(human)
Definition	Homo sapiens checkpoint kinase 1 (CHEK1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017017146.1

ORF Insert Sequence:

ATGGCAGTGC CCTTTGTGGA AGACTGGGAC TTGGTGCAAA CCCTGGGAGA AGGTGCCTAT 
GGAGAAGTTC AACTTGCTGT GAATAGAGTA ACTGAAGAAG CAGTCGCAGT GAAGATTGTA 
GATATGAAGC GTGCCGTAGA CTGTCCAGAA AATATTAAGA AAGAGATCTG TATCAATAAA 
ATGCTAAATC ATGAAAATGT AGTAAAATTC TATGGTCACA GGAGAGAAGG CAATATCCAA 
TATTTATTTC TGGAGTACTG TAGTGGAGGA GAGCTTTTTG ACAGAATAGA GCCAGACATA 
GGCATGCCTG AACCAGATGC TCAGAGATTC TTCCATCAAC TCATGGCAGG GGTGGTTTAT 
CTGCATGGTA TTGGAATAAC TCACAGGGAT ATTAAACCAG AAAATCTTCT GTTGGATGAA 
AGGGATAACC TCAAAATCTC AGACTTTGGC TTGGCAACAG TATTTCGGTA TAATAATCGT 
GAGCGTTTGT TGAACAAGAT GTGTGGTACT TTACCATATG TTGCTCCAGA ACTTCTGAAG 
AGAAGAGAAT TTCATGCAGA ACCAGTTGAT GTTTGGTCCT GTGGAATAGT ACTTACTGCA 
ATGCTCGCTG GAGAATTGCC ATGGGACCAA CCCAGTGACA GCTGTCAGGA GTATTCTGAC 
TGGAAAGAAA AAAAAACATA CCTCAACCCT TGGAAAAAAA TCGATTCTGC TCCTCTAGCT 
CTGCTGCATA AAATCTTAGT TGAGAATCCA TCAGCAAGAA TTACCATTCC AGACATCAAA 
AAAGATAGAT GGTACAACAA ACCCCTCAAG AAAGGGGCAA AAAGGCCCCG AGTCACTTCA 
GGTGGTGTGT CAGAGTCTCC CAGTGGATTT TCTAAGCACA TTCAATCCAA TTTGGACTTC 
TCTCCAGTAA ACAGTGCTTC TAGTGAAGAA AATGTGAAGT ACTCCAGTTC TCAGCCAGAA 
CCCCGCACAG GTCTTTCCTT ATGGGATACC AGCCCCTCAT ACATTGATAA ATTGGTACAA 
GGGATCAGCT TTTCCCAGCC CACATGTCCT GATCATATGC TTTTGAATAG TCAGTTACTT 
GGCACCCCAG GATCCTCACA GAACCCCTGG CAGCGGTTGG TCAAAAGAAT GACACGATTC 
TTTACCAAAT TGGATGCAGA CAAATCTTAT CAATGCCTGA AAGAGACTTG TGAGAAGTTG 
GGCTATCAAT GGAAGAAAAG TTGTATGAAT CAGGTTACTA TATCAACAAC TGATAGGAGA 
AACAATAAAC TCATTTTCAA AGTGAATTTG TTAGAAATGG ATGATAAAAT ATTGGTTGAC 
TTCCGGCTTT CTAAGGGTGA TGGATTGGAG TTCAAGAGAC ACTTCCTGAA GATTAAAGGG 
AAGCTGATTG ATATTGTGAG CAGCCAGAAG ATTTGGCTTC CTGCCACATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHEK1 ( XM_011542560.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011540862.1 Homo sapiens checkpoint kinase 1 (CHEK1), transcript variant X1, mRNA.

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CloneID	OHu56853
Clone ID Related Accession (Same CDS sequence)	NM_001330427.1 , XM_011542560.2
Accession Version	XM_011542560.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1479bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase Chk1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011542560.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGTCCG CCGTCCTTAA ATCTCTTCAG CCAGGATCTC TCCCCGACTG CAAAGCAGCC 
CTGGGCGGGA GCGGCAACAT CTCCACGTCA CCCTTTTGGA GCCGCCGACA TTCAGAGGGG 
CAGGACACGG GAACGCGCGC TGTCTTGCTT TACGGCGCGG GTGCGCGAGT TTGCGGCAGC 
GTGACGCCCT CAAGTTTTGG CGGGAAAAGC GCTGCATTTG GATTCCTGCA GTGGTGGGCA 
AAGGACAGTC CGCCGAGGTG CTCGGTGGAG TCATGGCAGT GCCCTTTGTG GAAGACTGGG 
ACTTGGTGCA AACCCTGGGA GAAGGTGCCT ATGGAGAAGC CAGACATAGG CATGCCTGAA 
CCAGATGCTC AGAGATTCTT CCATCAACTC ATGGCAGGGG TGGTTTATCT GCATGGTATT 
GGAATAACTC ACAGGGATAT TAAACCAGAA AATCTTCTGT TGGATGAAAG GGATAACCTC 
AAAATCTCAG ACTTTGGCTT GGCAACAGTA TTTCGGTATA ATAATCGTGA GCGTTTGTTG 
AACAAGATGT GTGGTACTTT ACCATATGTT GCTCCAGAAC TTCTGAAGAG AAGAGAATTT 
CATGCAGAAC CAGTTGATGT TTGGTCCTGT GGAATAGTAC TTACTGCAAT GCTCGCTGGA 
GAATTGCCAT GGGACCAACC CAGTGACAGC TGTCAGGAGT ATTCTGACTG GAAAGAAAAA 
AAAACATACC TCAACCCTTG GAAAAAAATC GATTCTGCTC CTCTAGCTCT GCTGCATAAA 
ATCTTAGTTG AGAATCCATC AGCAAGAATT ACCATTCCAG ACATCAAAAA AGATAGATGG 
TACAACAAAC CCCTCAAGAA AGGGGCAAAA AGGCCCCGAG TCACTTCAGG TGGTGTGTCA 
GAGTCTCCCA GTGGATTTTC TAAGCACATT CAATCCAATT TGGACTTCTC TCCAGTAAAC 
AGTGCTTCTA GTGAAGAAAA TGTGAAGTAC TCCAGTTCTC AGCCAGAACC CCGCACAGGT 
CTTTCCTTAT GGGATACCAG CCCCTCATAC ATTGATAAAT TGGTACAAGG GATCAGCTTT 
TCCCAGCCCA CATGTCCTGA TCATATGCTT TTGAATAGTC AGTTACTTGG CACCCCAGGA 
TCCTCACAGA ACCCCTGGCA GCGGTTGGTC AAAAGAATGA CACGATTCTT TACCAAATTG 
GATGCAGACA AATCTTATCA ATGCCTGAAA GAGACTTGTG AGAAGTTGGG CTATCAATGG 
AAGAAAAGTT GTATGAATCA GGTTACTATA TCAACAACTG ATAGGAGAAA CAATAAACTC 
ATTTTCAAAG TGAATTTGTT AGAAATGGAT GATAAAATAT TGGTTGACTT CCGGCTTTCT 
AAGGGTGATG GATTGGAGTT CAAGAGACAC TTCCTGAAGA TTAAAGGGAA GCTGATTGAT 
ATTGTGAGCA GCCAGAAGAT TTGGCTTCCT GCCACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011540862.1
CDS	852..2330
Translation	MRSAVLKSLQPGSLPDCKAALGGSGNISTSPFWSRRHSEGQDTGTRAVLLYGAGARVCGSVTPSSFGGKSAAFGFLQWWAKDSPPRCSVESWQCPLWKTGTWCKPWEKVPMEKPDIGMPEPDAQRFFHQLMAGVVYLHGIGITHRDIKPENLLLDERDNLKISDFGLATVFRYNNRERLLNKMCGTLPYVAPELLKRREFHAEPVDVWSCGIVLTAMLAGELPWDQPSDSCQEYSDWKEKKTYLNPWKKIDSAPLALLHKILVENPSARITIPDIKKDRWYNKPLKKGAKRPRVTSGGVSESPSGFSKHIQSNLDFSPVNSASSEENVKYSSSQPEPRTGLSLWDTSPSYIDKLVQGISFSQPTCPDHMLLNSQLLGTPGSSQNPWQRLVKRMTRFFTKLDADKSYQCLKETCEKLGYQWKKSCMNQVTISTTDRRNNKLIFKVNLLEMDDKILVDFRLSKGDGLEFKRHFLKIKGKLIDIVSSQKIWLPAT

Target ORF information:

RefSeq Version	XM_011542560.2
Organism	Homo sapiens(human)
Definition	Homo sapiens checkpoint kinase 1 (CHEK1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011542560.2

ORF Insert Sequence:

ATGCGGTCCG CCGTCCTTAA ATCTCTTCAG CCAGGATCTC TCCCCGACTG CAAAGCAGCC 
CTGGGCGGGA GCGGCAACAT CTCCACGTCA CCCTTTTGGA GCCGCCGACA TTCAGAGGGG 
CAGGACACGG GAACGCGCGC TGTCTTGCTT TACGGCGCGG GTGCGCGAGT TTGCGGCAGC 
GTGACGCCCT CAAGTTTTGG CGGGAAAAGC GCTGCATTTG GATTCCTGCA GTGGTGGGCA 
AAGGACAGTC CGCCGAGGTG CTCGGTGGAG TCATGGCAGT GCCCTTTGTG GAAGACTGGG 
ACTTGGTGCA AACCCTGGGA GAAGGTGCCT ATGGAGAAGC CAGACATAGG CATGCCTGAA 
CCAGATGCTC AGAGATTCTT CCATCAACTC ATGGCAGGGG TGGTTTATCT GCATGGTATT 
GGAATAACTC ACAGGGATAT TAAACCAGAA AATCTTCTGT TGGATGAAAG GGATAACCTC 
AAAATCTCAG ACTTTGGCTT GGCAACAGTA TTTCGGTATA ATAATCGTGA GCGTTTGTTG 
AACAAGATGT GTGGTACTTT ACCATATGTT GCTCCAGAAC TTCTGAAGAG AAGAGAATTT 
CATGCAGAAC CAGTTGATGT TTGGTCCTGT GGAATAGTAC TTACTGCAAT GCTCGCTGGA 
GAATTGCCAT GGGACCAACC CAGTGACAGC TGTCAGGAGT ATTCTGACTG GAAAGAAAAA 
AAAACATACC TCAACCCTTG GAAAAAAATC GATTCTGCTC CTCTAGCTCT GCTGCATAAA 
ATCTTAGTTG AGAATCCATC AGCAAGAATT ACCATTCCAG ACATCAAAAA AGATAGATGG 
TACAACAAAC CCCTCAAGAA AGGGGCAAAA AGGCCCCGAG TCACTTCAGG TGGTGTGTCA 
GAGTCTCCCA GTGGATTTTC TAAGCACATT CAATCCAATT TGGACTTCTC TCCAGTAAAC 
AGTGCTTCTA GTGAAGAAAA TGTGAAGTAC TCCAGTTCTC AGCCAGAACC CCGCACAGGT 
CTTTCCTTAT GGGATACCAG CCCCTCATAC ATTGATAAAT TGGTACAAGG GATCAGCTTT 
TCCCAGCCCA CATGTCCTGA TCATATGCTT TTGAATAGTC AGTTACTTGG CACCCCAGGA 
TCCTCACAGA ACCCCTGGCA GCGGTTGGTC AAAAGAATGA CACGATTCTT TACCAAATTG 
GATGCAGACA AATCTTATCA ATGCCTGAAA GAGACTTGTG AGAAGTTGGG CTATCAATGG 
AAGAAAAGTT GTATGAATCA GGTTACTATA TCAACAACTG ATAGGAGAAA CAATAAACTC 
ATTTTCAAAG TGAATTTGTT AGAAATGGAT GATAAAATAT TGGTTGACTT CCGGCTTTCT 
AAGGGTGATG GATTGGAGTT CAAGAGACAC TTCCTGAAGA TTAAAGGGAA GCTGATTGAT 
ATTGTGAGCA GCCAGAAGAT TTGGCTTCCT GCCACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHEK1 ( NM_001244846.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001231775.1 Homo sapiens checkpoint kinase 1 (CHEK1), transcript variant 4, mRNA.

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CloneID	OHu27174
Clone ID Related Accession (Same CDS sequence)	NM_001244846.1
Accession Version	NM_001244846.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1329bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-31
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase Chk1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX419129.2, AW149126.1, CA868104.1, AK300082.1, DA567616.1, AK293143.1, BC004202.2, CA441277.1, BM455102.1, BX506561.1, BX114781.1, AI750792.1 and AA224205.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK293143.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2148874, SAMEA2149004 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCAGTGC CCTTTGTGGA AGACTGGGAC TTGGTGCAAA CCCTGGGAGA AGGTGCCTAT 
GGAGAAGTTC AACTTGCTGT GAATAGAGTA ACTGAAGAAG CAGTCGCAGT GAAGATTGTA 
GATATGAAGC GTGCCGTAGA CTGTCCAGAA AATATTAAGA AAGAGATCTG TATCAATAAA 
ATGCTAAATC ATGAAAATGT AGTAAAATTC TATGGTCACA GGAGAGAAGG CAATATCCAA 
TATTTATTTC TGGAGTACTG TAGTGGAGGA GAGCTTTTTG ACAGAATAGA GCCAGACATA 
GGCATGCCTG AACCAGATGC TCAGAGATTC TTCCATCAAC TCATGGCAGG GGTGGTTTAT 
CTGCATGGTA TTGGAATAAC TCACAGGGAT ATTAAACCAG AAAATCTTCT GTTGGATGAA 
AGGGATAACC TCAAAATCTC AGACTTTGGC TTGGCAACAG TATTTCGGTA TAATAATCGT 
GAGCGTTTGT TGAACAAGAT GTGTGGTACT TTACCATATG TTGCTCCAGA ACTTCTGAAG 
AGAAGAGAAT TTCATGCAGA ACCAGTTGAT GTTTGGTCCT GTGGAATAGT ACTTACTGCA 
ATGCTCGCTG GAGAATTGCC ATGGGACCAA CCCAGTGACA GCTGTCAGGA GTATTCTGAC 
TGGAAAGAAA AAAAAACATA CCTCAACCCT TGGAAAAAAA TCGATTCTGC TCCTCTAGCT 
CTGCTGCATA AAATCTTAGT TGAGAATCCA TCAGCAAGAA TTACCATTCC AGACATCAAA 
AAAGATAGAT GGTACAACAA ACCCCTCAAG AAAGGGGCAA AAAGGCCCCG AGTCACTTCA 
GGTGGTGTGT CAGAGTCTCC CAGTGGATTT TCTAAGCACA TTCAATCCAA TTTGGACTTC 
TCTCCAGTAA ACAGTGCTTC TAGTGAAGAA AATGTGAAGT ACTCCAGTTC TCAGCCAGAA 
CCCCGCACAG GTCTTTCCTT ATGGGATACC AGCCCCTCAT ACATTGATAA ATTGGTACAA 
GGGATCAGCT TTTCCCAGCC CACATGTCCT GATCATATGC TTTTGAATAG TCAGTTACTT 
GGCACCCCAG GATCCTCACA GAACCCCTGG CAGCGGTTGG TCAAAAGAAT GACACGATTC 
TTTACCAAAT TGGATGCAGA CAAATCTTAT CAATGCCTGA AAGAGACTTG TGAGAAGTTG 
GGCTATCAAT GGAAGAAAAG TTGTATGAAT CAGGGTGATG GATTGGAGTT CAAGAGACAC 
TTCCTGAAGA TTAAAGGGAA GCTGATTGAT ATTGTGAGCA GCCAGAAGAT TTGGCTTCCT 
GCCACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001231775.1
CDS	898..2226
Translation	MAVPFVEDWDLVQTLGEGAYGEVQLAVNRVTEEAVAVKIVDMKRAVDCPENIKKEICINKMLNHENVVKFYGHRREGNIQYLFLEYCSGGELFDRIEPDIGMPEPDAQRFFHQLMAGVVYLHGIGITHRDIKPENLLLDERDNLKISDFGLATVFRYNNRERLLNKMCGTLPYVAPELLKRREFHAEPVDVWSCGIVLTAMLAGELPWDQPSDSCQEYSDWKEKKTYLNPWKKIDSAPLALLHKILVENPSARITIPDIKKDRWYNKPLKKGAKRPRVTSGGVSESPSGFSKHIQSNLDFSPVNSASSEENVKYSSSQPEPRTGLSLWDTSPSYIDKLVQGISFSQPTCPDHMLLNSQLLGTPGSSQNPWQRLVKRMTRFFTKLDADKSYQCLKETCEKLGYQWKKSCMNQGDGLEFKRHFLKIKGKLIDIVSSQKIWLPAT

Target ORF information:

RefSeq Version	NM_001244846.1
Organism	Homo sapiens(human)
Definition	Homo sapiens checkpoint kinase 1 (CHEK1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001244846.1

ORF Insert Sequence:

ATGGCAGTGC CCTTTGTGGA AGACTGGGAC TTGGTGCAAA CCCTGGGAGA AGGTGCCTAT 
GGAGAAGTTC AACTTGCTGT GAATAGAGTA ACTGAAGAAG CAGTCGCAGT GAAGATTGTA 
GATATGAAGC GTGCCGTAGA CTGTCCAGAA AATATTAAGA AAGAGATCTG TATCAATAAA 
ATGCTAAATC ATGAAAATGT AGTAAAATTC TATGGTCACA GGAGAGAAGG CAATATCCAA 
TATTTATTTC TGGAGTACTG TAGTGGAGGA GAGCTTTTTG ACAGAATAGA GCCAGACATA 
GGCATGCCTG AACCAGATGC TCAGAGATTC TTCCATCAAC TCATGGCAGG GGTGGTTTAT 
CTGCATGGTA TTGGAATAAC TCACAGGGAT ATTAAACCAG AAAATCTTCT GTTGGATGAA 
AGGGATAACC TCAAAATCTC AGACTTTGGC TTGGCAACAG TATTTCGGTA TAATAATCGT 
GAGCGTTTGT TGAACAAGAT GTGTGGTACT TTACCATATG TTGCTCCAGA ACTTCTGAAG 
AGAAGAGAAT TTCATGCAGA ACCAGTTGAT GTTTGGTCCT GTGGAATAGT ACTTACTGCA 
ATGCTCGCTG GAGAATTGCC ATGGGACCAA CCCAGTGACA GCTGTCAGGA GTATTCTGAC 
TGGAAAGAAA AAAAAACATA CCTCAACCCT TGGAAAAAAA TCGATTCTGC TCCTCTAGCT 
CTGCTGCATA AAATCTTAGT TGAGAATCCA TCAGCAAGAA TTACCATTCC AGACATCAAA 
AAAGATAGAT GGTACAACAA ACCCCTCAAG AAAGGGGCAA AAAGGCCCCG AGTCACTTCA 
GGTGGTGTGT CAGAGTCTCC CAGTGGATTT TCTAAGCACA TTCAATCCAA TTTGGACTTC 
TCTCCAGTAA ACAGTGCTTC TAGTGAAGAA AATGTGAAGT ACTCCAGTTC TCAGCCAGAA 
CCCCGCACAG GTCTTTCCTT ATGGGATACC AGCCCCTCAT ACATTGATAA ATTGGTACAA 
GGGATCAGCT TTTCCCAGCC CACATGTCCT GATCATATGC TTTTGAATAG TCAGTTACTT 
GGCACCCCAG GATCCTCACA GAACCCCTGG CAGCGGTTGG TCAAAAGAAT GACACGATTC 
TTTACCAAAT TGGATGCAGA CAAATCTTAT CAATGCCTGA AAGAGACTTG TGAGAAGTTG 
GGCTATCAAT GGAAGAAAAG TTGTATGAAT CAGGGTGATG GATTGGAGTT CAAGAGACAC 
TTCCTGAAGA TTAAAGGGAA GCTGATTGAT ATTGTGAGCA GCCAGAAGAT TTGGCTTCCT 
GCCACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHEK1 ( NM_001330428.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001317357.1 Homo sapiens checkpoint kinase 1 (CHEK1), transcript variant 8, mRNA.

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CloneID	OHu56855
Clone ID Related Accession (Same CDS sequence)	NM_001330428.1
Accession Version	NM_001330428.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1149bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase Chk1 isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AP001132.4. On Aug 29, 2016 this sequence version replaced XM_011542563.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.119311.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGAAGC CAGACATAGG CATGCCTGAA CCAGATGCTC AGAGATTCTT CCATCAACTC 
ATGGCAGGGG TGGTTTATCT GCATGGTATT GGAATAACTC ACAGGGATAT TAAACCAGAA 
AATCTTCTGT TGGATGAAAG GGATAACCTC AAAATCTCAG ACTTTGGCTT GGCAACAGTA 
TTTCGGTATA ATAATCGTGA GCGTTTGTTG AACAAGATGT GTGGTACTTT ACCATATGTT 
GCTCCAGAAC TTCTGAAGAG AAGAGAATTT CATGCAGAAC CAGTTGATGT TTGGTCCTGT 
GGAATAGTAC TTACTGCAAT GCTCGCTGGA GAATTGCCAT GGGACCAACC CAGTGACAGC 
TGTCAGGAGT ATTCTGACTG GAAAGAAAAA AAAACATACC TCAACCCTTG GAAAAAAATC 
GATTCTGCTC CTCTAGCTCT GCTGCATAAA ATCTTAGTTG AGAATCCATC AGCAAGAATT 
ACCATTCCAG ACATCAAAAA AGATAGATGG TACAACAAAC CCCTCAAGAA AGGGGCAAAA 
AGGCCCCGAG TCACTTCAGG TGGTGTGTCA GAGTCTCCCA GTGGATTTTC TAAGCACATT 
CAATCCAATT TGGACTTCTC TCCAGTAAAC AGTGCTTCTA GTGAAGAAAA TGTGAAGTAC 
TCCAGTTCTC AGCCAGAACC CCGCACAGGT CTTTCCTTAT GGGATACCAG CCCCTCATAC 
ATTGATAAAT TGGTACAAGG GATCAGCTTT TCCCAGCCCA CATGTCCTGA TCATATGCTT 
TTGAATAGTC AGTTACTTGG CACCCCAGGA TCCTCACAGA ACCCCTGGCA GCGGTTGGTC 
AAAAGAATGA CACGATTCTT TACCAAATTG GATGCAGACA AATCTTATCA ATGCCTGAAA 
GAGACTTGTG AGAAGTTGGG CTATCAATGG AAGAAAAGTT GTATGAATCA GGTTACTATA 
TCAACAACTG ATAGGAGAAA CAATAAACTC ATTTTCAAAG TGAATTTGTT AGAAATGGAT 
GATAAAATAT TGGTTGACTT CCGGCTTTCT AAGGGTGATG GATTGGAGTT CAAGAGACAC 
TTCCTGAAGA TTAAAGGGAA GCTGATTGAT ATTGTGAGCA GCCAGAAGAT TTGGCTTCCT 
GCCACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001317357.1
CDS	498..1646
Translation	MEKPDIGMPEPDAQRFFHQLMAGVVYLHGIGITHRDIKPENLLLDERDNLKISDFGLATVFRYNNRERLLNKMCGTLPYVAPELLKRREFHAEPVDVWSCGIVLTAMLAGELPWDQPSDSCQEYSDWKEKKTYLNPWKKIDSAPLALLHKILVENPSARITIPDIKKDRWYNKPLKKGAKRPRVTSGGVSESPSGFSKHIQSNLDFSPVNSASSEENVKYSSSQPEPRTGLSLWDTSPSYIDKLVQGISFSQPTCPDHMLLNSQLLGTPGSSQNPWQRLVKRMTRFFTKLDADKSYQCLKETCEKLGYQWKKSCMNQVTISTTDRRNNKLIFKVNLLEMDDKILVDFRLSKGDGLEFKRHFLKIKGKLIDIVSSQKIWLPAT

Target ORF information:

RefSeq Version	NM_001330428.1
Organism	Homo sapiens(human)
Definition	Homo sapiens checkpoint kinase 1 (CHEK1), transcript variant 8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001330428.1

ORF Insert Sequence:

ATGGAGAAGC CAGACATAGG CATGCCTGAA CCAGATGCTC AGAGATTCTT CCATCAACTC 
ATGGCAGGGG TGGTTTATCT GCATGGTATT GGAATAACTC ACAGGGATAT TAAACCAGAA 
AATCTTCTGT TGGATGAAAG GGATAACCTC AAAATCTCAG ACTTTGGCTT GGCAACAGTA 
TTTCGGTATA ATAATCGTGA GCGTTTGTTG AACAAGATGT GTGGTACTTT ACCATATGTT 
GCTCCAGAAC TTCTGAAGAG AAGAGAATTT CATGCAGAAC CAGTTGATGT TTGGTCCTGT 
GGAATAGTAC TTACTGCAAT GCTCGCTGGA GAATTGCCAT GGGACCAACC CAGTGACAGC 
TGTCAGGAGT ATTCTGACTG GAAAGAAAAA AAAACATACC TCAACCCTTG GAAAAAAATC 
GATTCTGCTC CTCTAGCTCT GCTGCATAAA ATCTTAGTTG AGAATCCATC AGCAAGAATT 
ACCATTCCAG ACATCAAAAA AGATAGATGG TACAACAAAC CCCTCAAGAA AGGGGCAAAA 
AGGCCCCGAG TCACTTCAGG TGGTGTGTCA GAGTCTCCCA GTGGATTTTC TAAGCACATT 
CAATCCAATT TGGACTTCTC TCCAGTAAAC AGTGCTTCTA GTGAAGAAAA TGTGAAGTAC 
TCCAGTTCTC AGCCAGAACC CCGCACAGGT CTTTCCTTAT GGGATACCAG CCCCTCATAC 
ATTGATAAAT TGGTACAAGG GATCAGCTTT TCCCAGCCCA CATGTCCTGA TCATATGCTT 
TTGAATAGTC AGTTACTTGG CACCCCAGGA TCCTCACAGA ACCCCTGGCA GCGGTTGGTC 
AAAAGAATGA CACGATTCTT TACCAAATTG GATGCAGACA AATCTTATCA ATGCCTGAAA 
GAGACTTGTG AGAAGTTGGG CTATCAATGG AAGAAAAGTT GTATGAATCA GGTTACTATA 
TCAACAACTG ATAGGAGAAA CAATAAACTC ATTTTCAAAG TGAATTTGTT AGAAATGGAT 
GATAAAATAT TGGTTGACTT CCGGCTTTCT AAGGGTGATG GATTGGAGTT CAAGAGACAC 
TTCCTGAAGA TTAAAGGGAA GCTGATTGAT ATTGTGAGCA GCCAGAAGAT TTGGCTTCCT 
GCCACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHEK1 ( XM_011542562.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011540864.1 Homo sapiens checkpoint kinase 1 (CHEK1), transcript variant X4, mRNA.

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CloneID	OHu56854
Clone ID Related Accession (Same CDS sequence)	XM_011542562.2
Accession Version	XM_011542562.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1230bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase Chk1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011542562.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGAGGT GCTCGGTGGA GTCATGGCAG TGCCCTTTGT GGAAGACTGG GACTTGGTGC 
AAACCCTGGG AGAAGGTGCC TATGGAGAAG CCAGACATAG GCATGCCTGA ACCAGATGCT 
CAGAGATTCT TCCATCAACT CATGGCAGGG GTGGTTTATC TGCATGGTAT TGGAATAACT 
CACAGGGATA TTAAACCAGA AAATCTTCTG TTGGATGAAA GGGATAACCT CAAAATCTCA 
GACTTTGGCT TGGCAACAGT ATTTCGGTAT AATAATCGTG AGCGTTTGTT GAACAAGATG 
TGTGGTACTT TACCATATGT TGCTCCAGAA CTTCTGAAGA GAAGAGAATT TCATGCAGAA 
CCAGTTGATG TTTGGTCCTG TGGAATAGTA CTTACTGCAA TGCTCGCTGG AGAATTGCCA 
TGGGACCAAC CCAGTGACAG CTGTCAGGAG TATTCTGACT GGAAAGAAAA AAAAACATAC 
CTCAACCCTT GGAAAAAAAT CGATTCTGCT CCTCTAGCTC TGCTGCATAA AATCTTAGTT 
GAGAATCCAT CAGCAAGAAT TACCATTCCA GACATCAAAA AAGATAGATG GTACAACAAA 
CCCCTCAAGA AAGGGGCAAA AAGGCCCCGA GTCACTTCAG GTGGTGTGTC AGAGTCTCCC 
AGTGGATTTT CTAAGCACAT TCAATCCAAT TTGGACTTCT CTCCAGTAAA CAGTGCTTCT 
AGTGAAGAAA ATGTGAAGTA CTCCAGTTCT CAGCCAGAAC CCCGCACAGG TCTTTCCTTA 
TGGGATACCA GCCCCTCATA CATTGATAAA TTGGTACAAG GGATCAGCTT TTCCCAGCCC 
ACATGTCCTG ATCATATGCT TTTGAATAGT CAGTTACTTG GCACCCCAGG ATCCTCACAG 
AACCCCTGGC AGCGGTTGGT CAAAAGAATG ACACGATTCT TTACCAAATT GGATGCAGAC 
AAATCTTATC AATGCCTGAA AGAGACTTGT GAGAAGTTGG GCTATCAATG GAAGAAAAGT 
TGTATGAATC AGGTTACTAT ATCAACAACT GATAGGAGAA ACAATAAACT CATTTTCAAA 
GTGAATTTGT TAGAAATGGA TGATAAAATA TTGGTTGACT TCCGGCTTTC TAAGGGTGAT 
GGATTGGAGT TCAAGAGACA CTTCCTGAAG ATTAAAGGGA AGCTGATTGA TATTGTGAGC 
AGCCAGAAGA TTTGGCTTCC TGCCACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011540864.1
CDS	351..1580
Translation	MPRCSVESWQCPLWKTGTWCKPWEKVPMEKPDIGMPEPDAQRFFHQLMAGVVYLHGIGITHRDIKPENLLLDERDNLKISDFGLATVFRYNNRERLLNKMCGTLPYVAPELLKRREFHAEPVDVWSCGIVLTAMLAGELPWDQPSDSCQEYSDWKEKKTYLNPWKKIDSAPLALLHKILVENPSARITIPDIKKDRWYNKPLKKGAKRPRVTSGGVSESPSGFSKHIQSNLDFSPVNSASSEENVKYSSSQPEPRTGLSLWDTSPSYIDKLVQGISFSQPTCPDHMLLNSQLLGTPGSSQNPWQRLVKRMTRFFTKLDADKSYQCLKETCEKLGYQWKKSCMNQVTISTTDRRNNKLIFKVNLLEMDDKILVDFRLSKGDGLEFKRHFLKIKGKLIDIVSSQKIWLPAT

Target ORF information:

RefSeq Version	XM_011542562.2
Organism	Homo sapiens(human)
Definition	Homo sapiens checkpoint kinase 1 (CHEK1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011542562.2

ORF Insert Sequence:

ATGCCGAGGT GCTCGGTGGA GTCATGGCAG TGCCCTTTGT GGAAGACTGG GACTTGGTGC 
AAACCCTGGG AGAAGGTGCC TATGGAGAAG CCAGACATAG GCATGCCTGA ACCAGATGCT 
CAGAGATTCT TCCATCAACT CATGGCAGGG GTGGTTTATC TGCATGGTAT TGGAATAACT 
CACAGGGATA TTAAACCAGA AAATCTTCTG TTGGATGAAA GGGATAACCT CAAAATCTCA 
GACTTTGGCT TGGCAACAGT ATTTCGGTAT AATAATCGTG AGCGTTTGTT GAACAAGATG 
TGTGGTACTT TACCATATGT TGCTCCAGAA CTTCTGAAGA GAAGAGAATT TCATGCAGAA 
CCAGTTGATG TTTGGTCCTG TGGAATAGTA CTTACTGCAA TGCTCGCTGG AGAATTGCCA 
TGGGACCAAC CCAGTGACAG CTGTCAGGAG TATTCTGACT GGAAAGAAAA AAAAACATAC 
CTCAACCCTT GGAAAAAAAT CGATTCTGCT CCTCTAGCTC TGCTGCATAA AATCTTAGTT 
GAGAATCCAT CAGCAAGAAT TACCATTCCA GACATCAAAA AAGATAGATG GTACAACAAA 
CCCCTCAAGA AAGGGGCAAA AAGGCCCCGA GTCACTTCAG GTGGTGTGTC AGAGTCTCCC 
AGTGGATTTT CTAAGCACAT TCAATCCAAT TTGGACTTCT CTCCAGTAAA CAGTGCTTCT 
AGTGAAGAAA ATGTGAAGTA CTCCAGTTCT CAGCCAGAAC CCCGCACAGG TCTTTCCTTA 
TGGGATACCA GCCCCTCATA CATTGATAAA TTGGTACAAG GGATCAGCTT TTCCCAGCCC 
ACATGTCCTG ATCATATGCT TTTGAATAGT CAGTTACTTG GCACCCCAGG ATCCTCACAG 
AACCCCTGGC AGCGGTTGGT CAAAAGAATG ACACGATTCT TTACCAAATT GGATGCAGAC 
AAATCTTATC AATGCCTGAA AGAGACTTGT GAGAAGTTGG GCTATCAATG GAAGAAAAGT 
TGTATGAATC AGGTTACTAT ATCAACAACT GATAGGAGAA ACAATAAACT CATTTTCAAA 
GTGAATTTGT TAGAAATGGA TGATAAAATA TTGGTTGACT TCCGGCTTTC TAAGGGTGAT 
GGATTGGAGT TCAAGAGACA CTTCCTGAAG ATTAAAGGGA AGCTGATTGA TATTGTGAGC 
AGCCAGAAGA TTTGGCTTCC TGCCACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	CHEK1
Entrez Gene ID	1111
Full Name	checkpoint kinase 1
Synonyms	CHK1
General protein information	Preferred Names checkpoint kinase 1 Names serine/threonine-protein kinase Chk1 CHK1 checkpoint homolog Checkpoint, S. pombe, homolog of, 1 Chk1-S cell cycle checkpoint kinase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 11 Map Location: 11q24.2
Summary	The protein encoded by this gene belongs to the Ser/Thr protein kinase family. It is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. This protein acts to integrate signals from ATM and ATR, two cell cycle proteins involved in DNA damage responses, that also associate with chromatin in meiotic prophase I. Phosphorylation of CDC25A protein phosphatase by this protein is required for cells to delay cell cycle progression in response to double-strand DNA breaks. Several alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Oct 2011].
Disorder MIM:	603078

Bos taurus (cattle)	CHEK1	NP_001091492.1
Gallus gallus (chicken)	STT3A	NP_989676.1
Saccharomyces cerevisiae (baker's yeast)	CHK1	NP_009833.1
Eremothecium gossypii	AGOS_AEL185C	NP_984676.1
Magnaporthe oryzae (rice blast fungus)	MGG_03729	XP_003716151.1
Xenopus tropicalis (tropical clawed frog)	chek1	XP_002937561.2
Homo sapiens (human)	CHEK1	NP_001265.2
Rattus norvegicus (Norway rat)	Chek1	NP_536325.1
Caenorhabditis elegans (roundworm)	chk-1	NP_001024233.1
Kluyveromyces lactis	KLLA0F01276g	XP_455137.1
Schizosaccharomyces pombe (fission yeast)	chk1	NP_588070.1
	LOC713358	XP_001111431.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008118	XP_317338.4
Pan troglodytes (chimpanzee)	CHEK1	XP_001146296.1
Mus musculus (house mouse)	Chek1	NP_031717.2
Neurospora crassa	NCU08346	XP_965249.2
Canis lupus familiaris (dog)	CHEK1	XP_852191.1
Danio rerio (zebrafish)	chek1	NP_956487.1
Drosophila melanogaster (fruit fly)	grp	NP_723986.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

CHEK1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

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