KERA cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	KERA
Entrez Gene ID	11081
Full Name	keratocan
Synonyms	CNA2,KTN,SLRR2B
General protein information	Preferred Names keratocan Names keratocan keratan sulfate proteoglycan keratocan
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 12 Map Location: 12q21.33
Summary	The protein encoded by this gene is a keratan sulfate proteoglycan that is involved in corneal transparency. Defects in this gene are a cause of autosomal recessive cornea plana 2 (CNA2).[provided by RefSeq, May 2010].
Disorder MIM:	603288
Disorder Html:	Cornea plana 2, autosomal recessive, 217300 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_007035.3	NP_008966.1	keratocan precursor

Pathways from BioSystems

Homologies

	KERA	XP_001102581.1
Danio rerio (zebrafish)	kera	NP_001020719.1
Canis lupus familiaris (dog)	KERA	XP_005629277.1
Gallus gallus (chicken)	KERA	NP_989507.1
Rattus norvegicus (Norway rat)	Kera	NP_001101557.1
Bos taurus (cattle)	KERA	NP_776335.1
Homo sapiens (human)	KERA	NP_008966.1
Pan troglodytes (chimpanzee)	KERA	XP_001135135.1
Mus musculus (house mouse)	Kera	NP_032464.1
Xenopus tropicalis (tropical clawed frog)	kera	XP_002937114.1

Gene Ontology
Bibliography

Related articles in PubMed

Analysis of KERA in four families with cornea plana identifies two novel mutations.
Dudakova L, Vercruyssen JHJ, Balikova I, Postolache L, Leroy BP, Skalicka P, Liskova P
Acta ophthalmologica96(1)e87-e91(2018 Feb)

Corneal ectasia in a boy with homozygous KERA mutation.
Khan AO
Ophthalmic genetics39(1)141-143(2018 Jan-Feb)

A Novel KERA Mutation in a Case of Autosomal Recessive Cornea Plana With Primary Angle-Closure Glaucoma.
Kumari D, Tiwari A, Choudhury M, Kumar A, Rao A, Dixit M
Journal of glaucoma25(2)e106-9(2016 Feb)

Case report: a novel KERA mutation associated with cornea plana and its predicted effect on protein function.
Roos L, Bertelsen B, Harris P, Bygum A, Jensen H, Gr?nskov K, T?mer Z
BMC medical genetics1640(2015 Jun)

Mutation in KERA identified by linkage analysis and targeted resequencing in a pedigree with premature atherosclerosis.
Maiwald S, Sivapalaratnam S, Motazacker MM, van Capelleveen JC, Bot I, de Jager SC, van Eck M, Jolley J, Kuiper J, Stephens J, Albers CA, Vosmeer CR, Kruize H, Geerke DP, van der Wal AC, van der Loos CM, Kastelein JJ, Trip MD, Ouwehand WH, Dallinga-Thie GM, Hovingh GK
PloS one9(5)e98289(2014)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The mutation that we report here leads to the deletion of a conserved amino acid (p.Phe125del) from the third LRR motif of the keratocan protein, which might lead to an abnormal tertiary structure of the protein, thereby leading to the disease.
Title: A Novel KERA Mutation in a Case of Autosomal Recessive Cornea Plana With Primary Angle-Closure Glaucoma.

a novel KERA variant, p.(Ile225Thr), was detected that segregates with Cornea plana in the homozygous form.
Title: Case report: a novel KERA mutation associated with cornea plana and its predicted effect on protein function.

Corneal endothelial disorders were found with compound mutations in KERA
Title: Corneal endothelial findings in a Czech patient with compound heterozygous mutations in KERA.

rare variant in KERA was identified in a large kindred with premature atherosclerosis
Title: Mutation in KERA identified by linkage analysis and targeted resequencing in a pedigree with premature atherosclerosis.

Observational study of gene-disease association. (HuGE Navigator)
Title: Candidate gene study to investigate the genetic determinants of normal variation in central corneal thickness.

The following KERA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the KERA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHu18735	NM_007035.3 Latest version!	Homo sapiens keratocan (KERA), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

KERA ( NM_007035.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_008966.1 Homo sapiens keratocan (KERA), mRNA.

Price & Availability↑

CloneID	OHu18735
Clone ID Related Accession (Same CDS sequence)	NM_007035.3
Accession Version	NM_007035.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1059bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Homo sapiens(human)
Product	keratocan precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC078873.22, CV571245.1, BC032667.2 and BM722433.1. This sequence is a reference standard in the RefSeqGene project. On Apr 22, 2005 this sequence version replaced NM_007035.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF205403.1, AK314990.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2146982, SAMEA2155371 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCAGGCA CAATCTGTTT CATCATGTGG GTGTTATTCA TAACAGACAC TGTGTGGTCT 
AGAAGTGTGA GGCAGGTCTA TGAAGTACAT GATTCAGATG ATTGGACTAT TCATGACTTC 
GAGTGTCCCA TGGAATGTTT CTGCCCACCC AGTTTTCCTA CTGCTTTATA TTGTGAAAAT 
AGAGGTCTCA AAGAAATTCC TGCTATTCCT TCAAGAATTT GGTATCTTTA TCTTCAAAAC 
AACCTGATAG AAACCATTCC TGAAAAGCCA TTTGAGAATG CCACCCAGCT AAGATGGATA 
AATCTAAACA AGAACAAAAT AACCAACTAC GGAATTGAAA AAGGAGCCCT AAGCCAGCTG 
AAGAAGTTGC TCTTCTTATT TCTGGAAGAT AATGAGCTAG AGGAGGTACC TTCTCCATTG 
CCAAGAAGTT TAGAACAATT ACAATTAGCT AGAAATAAGG TGTCCAGAAT TCCTCAAGGG 
ACCTTTAGCA ATCTGGAGAA CCTGACCCTT CTTGACCTAC AGAACAACAA ATTAGTGGAC 
AATGCCTTTC AAAGAGACAC TTTTAAAGGA CTCAAGAATC TCATGCAGCT AAACATGGCC 
AAGAATGCCC TGAGGAATAT GCCTCCAAGA TTACCAGCCA ATACAATGCA GTTGTTTTTA 
GACAACAATT CCATTGAAGG AATACCAGAA AATTATTTTA ATGTGATTCC TAAAGTGGCC 
TTTTTGAGAC TAAATCACAA CAAACTGTCA GATGAGGGTC TCCCATCAAG AGGATTTGAT 
GTATCATCAA TTCTAGATCT TCAACTGTCG CACAATCAAC TCACAAAGGT TCCCCGAATC 
AGTGCTCATC TGCAGCACCT TCACCTTGAT CATAACAAAA TTAAAAGTGT GAATGTCTCT 
GTAATATGTC CCAGCCCATC CATGCTGCCT GCAGAACGAG ATTCCTTCAG TTATGGACCT 
CATCTTCGCT ACCTCCGTCT GGATGGAAAT GAAATCAAAC CACCAATTCC AATGGCTTTA 
ATGACCTGCT TCAGACTTCT GCAGGCTGTC ATTATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_008966.1
CDS	620..1678
Translation	MAGTICFIMWVLFITDTVWSRSVRQVYEVHDSDDWTIHDFECPMECFCPPSFPTALYCENRGLKEIPAIPSRIWYLYLQNNLIETIPEKPFENATQLRWINLNKNKITNYGIEKGALSQLKKLLFLFLEDNELEEVPSPLPRSLEQLQLARNKVSRIPQGTFSNLENLTLLDLQNNKLVDNAFQRDTFKGLKNLMQLNMAKNALRNMPPRLPANTMQLFLDNNSIEGIPENYFNVIPKVAFLRLNHNKLSDEGLPSRGFDVSSILDLQLSHNQLTKVPRISAHLQHLHLDHNKIKSVNVSVICPSPSMLPAERDSFSYGPHLRYLRLDGNEIKPPIPMALMTCFRLLQAVII

Target ORF information:

RefSeq Version	NM_007035.3
Organism	Homo sapiens(human)
Definition	Homo sapiens keratocan (KERA), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007035.3

ORF Insert Sequence:

ATGGCAGGCA CAATCTGTTT CATCATGTGG GTGTTATTCA TAACAGACAC TGTGTGGTCT 
AGAAGTGTGA GGCAGGTCTA TGAAGTACAT GATTCAGATG ATTGGACTAT TCATGACTTC 
GAGTGTCCCA TGGAATGTTT CTGCCCACCC AGTTTTCCTA CTGCTTTATA TTGTGAAAAT 
AGAGGTCTCA AAGAAATTCC TGCTATTCCT TCAAGAATTT GGTATCTTTA TCTTCAAAAC 
AACCTGATAG AAACCATTCC TGAAAAGCCA TTTGAGAATG CCACCCAGCT AAGATGGATA 
AATCTAAACA AGAACAAAAT AACCAACTAC GGAATTGAAA AAGGAGCCCT AAGCCAGCTG 
AAGAAGTTGC TCTTCTTATT TCTGGAAGAT AATGAGCTAG AGGAGGTACC TTCTCCATTG 
CCAAGAAGTT TAGAACAATT ACAATTAGCT AGAAATAAGG TGTCCAGAAT TCCTCAAGGG 
ACCTTTAGCA ATCTGGAGAA CCTGACCCTT CTTGACCTAC AGAACAACAA ATTAGTGGAC 
AATGCCTTTC AAAGAGACAC TTTTAAAGGA CTCAAGAATC TCATGCAGCT AAACATGGCC 
AAGAATGCCC TGAGGAATAT GCCTCCAAGA TTACCAGCCA ATACAATGCA GTTGTTTTTA 
GACAACAATT CCATTGAAGG AATACCAGAA AATTATTTTA ATGTGATTCC TAAAGTGGCC 
TTTTTGAGAC TAAATCACAA CAAACTGTCA GATGAGGGTC TCCCATCAAG AGGATTTGAT 
GTATCATCAA TTCTAGATCT TCAACTGTCG CACAATCAAC TCACAAAGGT TCCCCGAATC 
AGTGCTCATC TGCAGCACCT TCACCTTGAT CATAACAAAA TTAAAAGTGT GAATGTCTCT 
GTAATATGTC CCAGCCCATC CATGCTGCCT GCAGAACGAG ATTCCTTCAG TTATGGACCT 
CATCTTCGCT ACCTCCGTCT GGATGGAAAT GAAATCAAAC CACCAATTCC AATGGCTTTA 
ATGACCTGCT TCAGACTTCT GCAGGCTGTC ATTATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Analysis of KERA in four families with cornea plana identifies two novel mutations.
Acta ophthalmologica96(1)e87-e91(2018 Feb)
Dudakova L,Vercruyssen JHJ,Balikova I,Postolache L,Leroy BP,Skalicka P,Liskova P

Corneal ectasia in a boy with homozygous KERA mutation.
Ophthalmic genetics39(1)141-143(2018 Jan-Feb)
Khan AO

A Novel KERA Mutation in a Case of Autosomal Recessive Cornea Plana With Primary Angle-Closure Glaucoma.
Journal of glaucoma25(2)e106-9(2016 Feb)
Kumari D,Tiwari A,Choudhury M,Kumar A,Rao A,Dixit M

Case report: a novel KERA mutation associated with cornea plana and its predicted effect on protein function.
BMC medical genetics1640(2015 Jun)
Roos L,Bertelsen B,Harris P,Bygum A,Jensen H,Gr?nskov K,T?mer Z

Mutation in KERA identified by linkage analysis and targeted resequencing in a pedigree with premature atherosclerosis.
PloS one9(5)e98289(2014)
Maiwald S,Sivapalaratnam S,Motazacker MM,van Capelleveen JC,Bot I,de Jager SC,van Eck M,Jolley J,Kuiper J,Stephens J,Albers CA,Vosmeer CR,Kruize H,Geerke DP,van der Wal AC,van der Loos CM,Kastelein JJ,Trip MD,Ouwehand WH,Dallinga-Thie GM,Hovingh GK

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KERA cDNA ORF clone, Homo sapiens(human)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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