CHD4 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	CHD4
Entrez Gene ID	1108
Full Name	chromodomain helicase DNA binding protein 4
Synonyms	CHD-4,Mi-2b,Mi2-BETA,SIHIWES
General protein information	Preferred Names chromodomain helicase DNA binding protein 4 Names chromodomain-helicase-DNA-binding protein 4 ATP-dependent helicase CHD4 Mi-2 autoantigen 218 kDa protein
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 12 Map Location: 12p13.31
Summary	The product of this gene belongs to the SNF2/RAD54 helicase family. It represents the main component of the nucleosome remodeling and deacetylase complex and plays an important role in epigenetic transcriptional repression. Patients with dermatomyositis develop antibodies against this protein. Somatic mutations in this gene are associated with serous endometrial tumors. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2014].
Disorder MIM:	603277
Disorder Html:	Sifrim-Hitz-Weiss syndrome, 617159 (3)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017018727.1	XP_016874216.1	chromodomain-helicase-DNA-binding protein 4 isoform X10
NM_001297553.1	NP_001284482.1	chromodomain-helicase-DNA-binding protein 4 isoform 2
NM_001273.4	NP_001264.2	chromodomain-helicase-DNA-binding protein 4 isoform 1
XM_024448802.1	XP_024304570.1	chromodomain-helicase-DNA-binding protein 4 isoform X1
XM_006718958.1	XP_006719021.1	chromodomain-helicase-DNA-binding protein 4 isoform X2
XM_017018725.1	XP_016874214.1	chromodomain-helicase-DNA-binding protein 4 isoform X7
XM_017018726.1	XP_016874215.1	chromodomain-helicase-DNA-binding protein 4 isoform X9
XM_017018733.1	XP_016874222.1	chromodomain-helicase-DNA-binding protein 4 isoform X19
XM_006718962.1	XP_006719025.1	chromodomain-helicase-DNA-binding protein 4 isoform X11
NM_001297553.2	NP_001284482.1	chromodomain-helicase-DNA-binding protein 4 isoform 2
NM_001273.5	NP_001264.2	chromodomain-helicase-DNA-binding protein 4 isoform 1
NM_001363606.1	NP_001350535.1	chromodomain-helicase-DNA-binding protein 4 isoform 3
XM_024448804.1	XP_024304572.1	chromodomain-helicase-DNA-binding protein 4 isoform X4
XM_024448805.1	XP_024304573.1	chromodomain-helicase-DNA-binding protein 4 isoform X5
XM_006718961.2	XP_006719024.1	chromodomain-helicase-DNA-binding protein 4 isoform X12
XM_017018729.1	XP_016874218.1	chromodomain-helicase-DNA-binding protein 4 isoform X13
XM_005253668.3	XP_005253725.1	chromodomain-helicase-DNA-binding protein 4 isoform X14
XM_017018730.1	XP_016874219.1	chromodomain-helicase-DNA-binding protein 4 isoform X16
XM_017018731.1	XP_016874220.1	chromodomain-helicase-DNA-binding protein 4 isoform X17
XM_017018732.1	XP_016874221.1	chromodomain-helicase-DNA-binding protein 4 isoform X18
XM_017018734.1	XP_016874223.1	chromodomain-helicase-DNA-binding protein 4 isoform X20
NM_001273.3	NP_001264.2	chromodomain-helicase-DNA-binding protein 4 isoform 1
XM_017018728.1	XP_016874217.1	chromodomain-helicase-DNA-binding protein 4 isoform X11
XM_024448803.1	XP_024304571.1	chromodomain-helicase-DNA-binding protein 4 isoform X3
XM_006718959.1	XP_006719022.1	chromodomain-helicase-DNA-binding protein 4 isoform X6
XM_006718960.1	XP_006719023.1	chromodomain-helicase-DNA-binding protein 4 isoform X8
NM_001363606.2	NP_001350535.1	chromodomain-helicase-DNA-binding protein 4 isoform 3

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Chd4	NP_666091.1
Caenorhabditis elegans (roundworm)	chd-3	NP_510140.1
Danio rerio (zebrafish)	chd4a	NP_001038323.1
Canis lupus familiaris (dog)	CHD4	XP_867754.2
Danio rerio (zebrafish)	chd4b	XP_685699.4
Arabidopsis thaliana (thale cress)	PKL	NP_565587.1
Arabidopsis thaliana (thale cress)	PKR2	NP_194918.2
Homo sapiens (human)	CHD4	NP_001264.2
Bos taurus (cattle)	CHD4	NP_001193430.1
Gallus gallus (chicken)	CHD4	XP_003640489.2
Caenorhabditis elegans (roundworm)	let-418	NP_504523.1
Xenopus tropicalis (tropical clawed frog)	chd4	NP_001096166.1
	CHD4	XP_001107252.2
Rattus norvegicus (Norway rat)	Chd4	XP_006237457.1

Gene Ontology
Bibliography

Related articles in PubMed

CHD4-mediated loss of E-cadherin determines metastatic ability in triple-negative breast cancer cells.
Luo CW, Wu CC, Chang SJ, Chang TM, Chen TY, Chai CY, Chang CL, Hou MF, Pan MR
Experimental cell research(2018 Jan)

Transcription factor 19 interacts with histone 3 lysine 4 trimethylation and controls gluconeogenesis via the nucleosome-remodeling-deacetylase complex.
Sen S, Sanyal S, Srivastava DK, Dasgupta D, Roy S, Das C
The Journal of biological chemistry292(50)20362-20378(2017 Dec)

EZH2-, CHD4-, and IDH-linked epigenetic perturbation and its association with survival in glioma patients.
Zhang L, Liu Y, Wang M, Wu Z, Li N, Zhang J, Yang C
Journal of molecular cell biology(2017 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The findings identify that CHD4 deficiency preferentially impairs cell survival via increasing the level of p21.
Title: The NuRD complex-mediated p21 suppression facilitates chemoresistance in BRCA-proficient breast cancer.

CHD3 and CHD4 exhibit distinct nuclear localization patterns in unperturbed cells, revealing a subset of specific target genes.
Title: CHD3 and CHD4 form distinct NuRD complexes with different yet overlapping functionality.

CHD4 recruits repressive chromatin proteins to sites of DNA damage repair, including DNA methyltransferases where it imposes de novo DNA methylation. At TSGs, CHD4 retention helps maintain DNA hypermethylation-associated transcriptional silencing.
Title: CHD4 Has Oncogenic Functions in Initiating and Maintaining Epigenetic Suppression of Multiple Tumor Suppressor Genes.

this study identifies the Chd4-Tbx3 axis in controlling ESC fate and a role of H2A.Z in maintaining the stability of Chd4 proteins.
Title: The chromatin remodeler Chd4 maintains embryonic stem cell identity by controlling pluripotency- and differentiation-associated genes.

this work identifies CHD4 as an epigenetic coregulator of PAX3-FOXO1 activity, providing rational evidence for CHD4 as a potential therapeutic target in alveolar rhabdomyosarcoma.
Title: Helicase CHD4 is an epigenetic coregulator of PAX3-FOXO1 in alveolar rhabdomyosarcoma.

The following CHD4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CHD4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu28780	NM_001273.5 Latest version!	Homo sapiens chromodomain helicase DNA binding protein 4 (CHD4), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$679.50-$951.30 $1359.00
OHu103674	XM_024448802.1 Latest version!	Homo sapiens chromodomain helicase DNA binding protein 4 (CHD4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu103675	XM_024448803.1 Latest version!	Homo sapiens chromodomain helicase DNA binding protein 4 (CHD4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu103676	XM_024448804.1 Latest version!	Homo sapiens chromodomain helicase DNA binding protein 4 (CHD4), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu103677	XM_024448805.1 Latest version!	Homo sapiens chromodomain helicase DNA binding protein 4 (CHD4), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu28781	NM_001297553.2 Latest version!	Homo sapiens chromodomain helicase DNA binding protein 4 (CHD4), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu45954	NM_001363606.2 Latest version!	Homo sapiens chromodomain helicase DNA binding protein 4 (CHD4), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu28781	XM_005253668.3 Latest version!	Homo sapiens chromodomain helicase DNA binding protein 4 (CHD4), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu45950	XM_006718958.1 Latest version!	Homo sapiens chromodomain helicase DNA binding protein 4 (CHD4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu45951	XM_006718959.1 Latest version!	Homo sapiens chromodomain helicase DNA binding protein 4 (CHD4), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu45952	XM_006718960.1 Latest version!	Homo sapiens chromodomain helicase DNA binding protein 4 (CHD4), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu45953	XM_006718961.2 Latest version!	Homo sapiens chromodomain helicase DNA binding protein 4 (CHD4), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu45954	XM_006718962.1 Latest version!	Homo sapiens chromodomain helicase DNA binding protein 4 (CHD4), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu83470	XM_017018725.1 Latest version!	Homo sapiens chromodomain helicase DNA binding protein 4 (CHD4), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu83471	XM_017018726.1 Latest version!	Homo sapiens chromodomain helicase DNA binding protein 4 (CHD4), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu83472	XM_017018727.1 Latest version!	Homo sapiens chromodomain helicase DNA binding protein 4 (CHD4), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu83473	XM_017018728.1 Latest version!	Homo sapiens chromodomain helicase DNA binding protein 4 (CHD4), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu83474	XM_017018729.1 Latest version!	Homo sapiens chromodomain helicase DNA binding protein 4 (CHD4), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu83475	XM_017018730.1 Latest version!	Homo sapiens chromodomain helicase DNA binding protein 4 (CHD4), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu83476	XM_017018731.1 Latest version!	Homo sapiens chromodomain helicase DNA binding protein 4 (CHD4), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu83477	XM_017018732.1 Latest version!	Homo sapiens chromodomain helicase DNA binding protein 4 (CHD4), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu83478	XM_017018733.1 Latest version!	Homo sapiens chromodomain helicase DNA binding protein 4 (CHD4), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu83479	XM_017018734.1 Latest version!	Homo sapiens chromodomain helicase DNA binding protein 4 (CHD4), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
View Old Accession Versions >>
OHu28781	NM_001297553.1	Homo sapiens chromodomain helicase DNA binding protein 4 (CHD4), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu28780	NM_001273.4	Homo sapiens chromodomain helicase DNA binding protein 4 (CHD4), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$679.50-$951.30 $1359.00
OHu28780	NM_001273.3	Homo sapiens chromodomain helicase DNA binding protein 4 (CHD4), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$679.50-$951.30 $1359.00
OHu45954	NM_001363606.1	Homo sapiens chromodomain helicase DNA binding protein 4 (CHD4), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CHD4 ( NM_001273.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_001264.2 Homo sapiens chromodomain helicase DNA binding protein 4 (CHD4), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu28780
Clone ID Related Accession (Same CDS sequence)	NM_001273.4 , NM_001273.5 , NM_001273.3
Accession Version	NM_001273.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5739bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-26
Organism	Homo sapiens(human)
Product	chromodomain-helicase-DNA-binding protein 4 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC006064.10, AA099889.1, BP234473.1, X86691.1 and BC038596.2. This sequence is a reference standard in the RefSeqGene project. On May 24, 2018 this sequence version replaced NM_001273.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X86691.1, SRR1803615.127809.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGTCGG GCCTGGGCTC CCCGTCCCCC TGCTCGGCGG GCAGTGAGGA GGAGGATATG 
GATGCACTTT TGAACAACAG CCTGCCCCCA CCCCACCCAG AAAATGAAGA GGACCCAGAA 
GAGGATTTGT CAGAAACAGA GACTCCAAAG CTCAAGAAGA AGAAAAAGCC TAAGAAACCT 
CGGGACCCTA AAATCCCTAA GAGCAAGCGC CAAAAAAAGG AGCGTATGCT CTTATGCCGG 
CAGCTGGGGG ACAGCTCTGG GGAGGGGCCA GAGTTTGTGG AGGAGGAGGA AGAGGTGGCT 
CTGCGCTCAG ACAGTGAGGG CAGCGACTAT ACTCCTGGCA AGAAGAAGAA GAAGAAGCTT 
GGACCTAAGA AAGAGAAGAA GAGCAAATCC AAGCGGAAGG AGGAGGAGGA GGAGGAGGAT 
GATGATGATG ATTCAAAGGA GCCTAAATCA TCTGCTCAGC TCCTGGAAGA CTGGGGCATG 
GAAGACATTG ACCACGTGTT CTCAGAGGAG GATTATCGAA CCCTCACCAA CTACAAGGCC 
TTCAGCCAGT TTGTCAGACC CCTCATTGCT GCCAAAAATC CCAAGATTGC TGTCTCCAAG 
ATGATGATGG TTTTGGGTGC AAAATGGCGG GAGTTCAGTA CCAATAACCC CTTCAAAGGC 
AGTTCTGGGG CATCAGTGGC AGCTGCGGCA GCAGCAGCGG TAGCTGTGGT GGAGAGCATG 
GTGACAGCCA CTGAGGTTGC ACCACCACCT CCCCCTGTGG AGGTGCCTAT CCGCAAGGCC 
AAGACCAAGG AGGGCAAAGG TCCCAATGCT CGGAGGAAGC CCAAGGGCAG CCCTCGTGTA 
CCTGATGCCA AGAAGCCTAA ACCCAAGAAA GTAGCTCCCC TGAAAATCAA GCTGGGAGGT 
TTTGGTTCCA AGCGTAAGAG ATCCTCGAGT GAGGATGATG ACTTAGATGT GGAATCTGAC 
TTCGATGATG CCAGTATCAA TAGCTATTCT GTTTCTGATG GTTCCACCAG CCGTAGTAGC 
CGCAGCCGCA AGAAACTCCG AACCACTAAA AAGAAAAAGA AAGGCGAGGA GGAGGTGACT 
GCTGTGGATG GTTATGAGAC AGACCACCAG GACTATTGCG AGGTGTGCCA GCAAGGCGGT 
GAGATCATCC TGTGTGATAC CTGTCCCCGT GCTTACCACA TGGTCTGCCT GGATCCCGAC 
ATGGAGAAGG CTCCCGAGGG CAAGTGGAGC TGCCCACACT GCGAGAAGGA AGGCATCCAG 
TGGGAAGCTA AAGAGGACAA TTCGGAGGGT GAGGAGATCC TGGAAGAGGT TGGGGGAGAC 
CTCGAAGAGG AGGATGACCA CCATATGGAA TTCTGTCGGG TCTGCAAGGA TGGTGGGGAA 
CTGCTCTGCT GTGATACCTG TCCTTCTTCC TACCACATCC ACTGCCTGAA TCCCCCACTT 
CCAGAGATCC CCAACGGTGA ATGGCTCTGT CCCCGTTGTA CGTGTCCAGC TCTGAAGGGC 
AAAGTGCAGA AGATCCTAAT CTGGAAGTGG GGTCAGCCAC CATCTCCCAC ACCAGTGCCT 
CGGCCTCCAG ATGCTGATCC CAACACGCCC TCCCCAAAGC CCTTGGAGGG GCGGCCAGAG 
CGGCAGTTCT TTGTGAAATG GCAAGGCATG TCTTACTGGC ACTGCTCCTG GGTTTCTGAA 
CTGCAGCTGG AGCTGCACTG TCAGGTGATG TTCCGAAACT ATCAGCGGAA GAATGATATG 
GATGAGCCAC CTTCTGGGGA CTTTGGTGGT GATGAAGAGA AAAGCCGAAA GCGAAAGAAC 
AAGGACCCTA AATTTGCAGA GATGGAGGAA CGCTTCTATC GCTATGGGAT AAAACCCGAG 
TGGATGATGA TCCACCGAAT CCTCAACCAC AGTGTGGACA AGAAGGGCCA CGTCCACTAC 
TTGATCAAGT GGCGGGACTT ACCTTACGAT CAGGCTTCTT GGGAGAGTGA GGATGTGGAG 
ATCCAGGATT ACGACCTGTT CAAGCAGAGC TATTGGAATC ACAGGGAGTT AATGAGGGGT 
GAGGAAGGCC GACCAGGCAA GAAGCTCAAG AAGGTGAAGC TTCGGAAGTT GGAGAGGCCT 
CCAGAAACGC CAACAGTTGA TCCAACAGTG AAGTATGAGC GACAGCCAGA GTACCTGGAT 
GCTACAGGTG GAACCCTGCA CCCCTATCAA ATGGAGGGCC TGAATTGGTT GCGCTTCTCC 
TGGGCTCAGG GCACTGACAC CATCTTGGCT GATGAGATGG GCCTTGGGAA AACTGTACAG 
ACAGCAGTCT TCCTGTATTC CCTTTACAAG GAGGGTCATT CCAAAGGCCC CTTCCTAGTG 
AGCGCCCCTC TTTCTACCAT CATCAACTGG GAGCGGGAGT TTGAAATGTG GGCTCCAGAC 
ATGTATGTCG TAACCTATGT GGGTGACAAG GACAGCCGTG CCATCATCCG AGAGAATGAG 
TTCTCCTTTG AAGACAATGC CATTCGTGGT GGCAAGAAGG CCTCCCGCAT GAAGAAAGAG 
GCATCTGTGA AATTCCATGT GCTGCTGACA TCCTATGAAT TGATCACCAT TGACATGGCT 
ATTTTGGGCT CTATTGATTG GGCCTGCCTC ATCGTGGATG AAGCCCATCG GCTGAAGAAC 
AATCAGTCTA AGTTCTTCCG GGTATTGAAT GGTTACTCAC TCCAGCACAA GCTGTTGCTG 
ACTGGGACAC CATTACAAAA CAATCTGGAA GAGTTGTTTC ATCTGCTCAA CTTTCTCACC 
CCCGAGAGGT TCCACAATTT GGAAGGTTTT TTGGAGGAGT TTGCTGACAT TGCCAAGGAG 
GACCAGATAA AAAAACTGCA TGACATGCTG GGGCCGCACA TGTTGCGGCG GCTCAAAGCC 
GATGTGTTCA AGAACATGCC CTCCAAGACA GAACTAATTG TGCGTGTGGA GCTGAGCCCT 
ATGCAGAAGA AATACTACAA GTACATCCTC ACTCGAAATT TTGAAGCACT CAATGCCCGA 
GGTGGTGGCA ACCAGGTGTC TCTGCTGAAT GTGGTGATGG ATCTTAAGAA GTGCTGCAAC 
CATCCATACC TCTTCCCTGT GGCTGCAATG GAAGCTCCTA AGATGCCTAA TGGCATGTAT 
GATGGCAGTG CCCTAATCAG AGCATCTGGG AAATTATTGC TGCTGCAGAA AATGCTCAAG 
AACCTTAAGG AGGGTGGGCA TCGTGTACTC ATCTTTTCCC AGATGACCAA GATGCTAGAC 
CTGCTAGAGG ATTTCTTGGA ACATGAAGGT TATAAATACG AACGCATCGA TGGTGGAATC 
ACTGGGAACA TGCGGCAAGA GGCCATTGAC CGCTTCAATG CACCGGGTGC TCAGCAGTTC 
TGCTTCTTGC TTTCCACTCG AGCTGGGGGC CTTGGAATCA ATCTGGCCAC TGCTGACACA 
GTTATTATCT ATGACTCTGA CTGGAACCCC CATAATGACA TTCAGGCCTT TAGCAGAGCT 
CACCGGATTG GGCAAAATAA AAAGGTAATG ATCTACCGGT TTGTGACCCG TGCGTCAGTG 
GAGGAGCGCA TCACGCAGGT GGCAAAGAAG AAAATGATGC TGACGCATCT AGTGGTGCGG 
CCTGGGCTGG GCTCCAAGAC TGGATCTATG TCCAAACAGG AGCTTGATGA TATCCTCAAA 
TTTGGCACTG AGGAACTATT CAAGGATGAA GCCACTGATG GAGGAGGAGA CAACAAAGAG 
GGAGAAGATA GCAGTGTTAT CCACTACGAT GATAAGGCCA TTGAACGGCT GCTAGACCGT 
AACCAGGATG AGACTGAAGA CACAGAATTG CAGGGCATGA ATGAATATTT GAGCTCATTC 
AAAGTGGCCC AGTATGTGGT ACGGGAAGAA GAAATGGGGG AGGAAGAGGA GGTAGAACGG 
GAAATCATTA AACAGGAAGA AAGTGTGGAT CCTGACTACT GGGAGAAATT GCTGCGGCAC 
CATTATGAGC AGCAGCAAGA AGATCTAGCC CGAAATCTGG GCAAAGGAAA AAGAATCCGT 
AAACAGGTCA ACTACAATGA TGGCTCCCAG GAGGACCGAG ATTGGCAGGA CGACCAGTCC 
GACAACCAGT CCGATTACTC AGTGGCTTCA GAGGAAGGTG ATGAAGACTT TGATGAACGT 
TCAGAAGCTC CCCGTAGGCC CAGTCGTAAG GGCCTGCGGA ATGATAAAGA TAAGCCATTG 
CCTCCTCTGT TGGCCCGTGT TGGTGGGAAT ATTGAAGTAC TTGGTTTTAA TGCTCGTCAG 
CGAAAAGCCT TTCTTAATGC AATTATGCGA TATGGTATGC CACCTCAGGA TGCTTTTACT 
ACCCAGTGGC TTGTAAGAGA CCTGCGAGGC AAATCAGAGA AAGAGTTCAA GGCATATGTC 
TCTCTTTTCA TGCGGCATTT ATGTGAGCCG GGGGCAGATG GGGCTGAGAC CTTTGCTGAT 
GGTGTCCCCC GAGAAGGCCT GTCTCGCCAG CATGTCCTTA CTAGAATTGG TGTTATGTCT 
TTGATTCGCA AGAAGGTTCA GGAGTTTGAA CATGTTAATG GGCGCTGGAG CATGCCTGAA 
CTGGCTGAGG TGGAGGAAAA CAAGAAGATG TCCCAGCCAG GGTCACCCTC CCCAAAAACT 
CCTACACCCT CCACTCCAGG GGACACGCAG CCCAACACTC CTGCACCTGT CCCACCTGCT 
GAAGATGGGA TAAAAATAGA GGAAAATAGC CTCAAAGAAG AAGAGAGCAT AGAAGGAGAA 
AAGGAGGTTA AATCTACAGC CCCTGAGACT GCCATTGAGT GTACACAGGC CCCTGCCCCT 
GCCTCAGAGG ATGAAAAGGT CGTTGTTGAA CCCCCTGAGG GAGAGGAGAA AGTGGAAAAG 
GCAGAGGTGA AGGAGAGAAC AGAGGAACCT ATGGAGACAG AGCCCAAAGG TGCTGCTGAT 
GTAGAGAAGG TGGAGGAAAA GTCAGCAATA GATCTGACCC CTATTGTGGT AGAAGACAAA 
GAAGAGAAGA AAGAAGAAGA AGAGAAAAAA GAGGTGATGC TTCAGAATGG AGAGACCCCC 
AAGGACCTGA ATGATGAGAA ACAGAAGAAA AATATTAAAC AACGTTTCAT GTTTAACATT 
GCAGATGGTG GTTTTACTGA GTTGCACTCC CTTTGGCAGA ATGAAGAGCG GGCAGCCACA 
GTTACCAAGA AGACTTATGA GATCTGGCAT CGACGGCATG ACTACTGGCT GCTAGCCGGC 
ATTATAAACC ATGGCTATGC CCGGTGGCAA GACATCCAGA ATGACCCACG CTATGCCATC 
CTCAATGAGC CTTTCAAGGG TGAAATGAAC CGTGGCAATT TCTTAGAGAT CAAGAATAAA 
TTTCTAGCTC GAAGGTTTAA GCTCTTAGAA CAAGCTCTGG TGATTGAGGA ACAGCTGCGC 
CGGGCTGCTT ACTTGAACAT GTCAGAAGAC CCTTCTCACC CTTCCATGGC CCTCAACACC 
CGCTTTGCTG AGGTGGAGTG TTTGGCGGAA AGTCATCAGC ACCTGTCCAA GGAGTCAATG 
GCAGGAAACA AGCCAGCCAA TGCAGTCCTG CACAAAGTTC TGAAACAGCT GGAAGAACTG 
CTGAGTGACA TGAAAGCTGA TGTGACTCGA CTCCCAGCTA CCATTGCCCG AATTCCCCCA 
GTTGCTGTGA GGTTACAGAT GTCAGAGCGT AACATTCTCA GCCGCCTGGC AAACCGGGCA 
CCCGAACCTA CCCCACAGCA GGTAGCCCAG CAGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001264.2
CDS	256..5994
Translation	MASGLGSPSPCSAGSEEEDMDALLNNSLPPPHPENEEDPEEDLSETETPKLKKKKKPKKPRDPKIPKSKRQKKERMLLCRQLGDSSGEGPEFVEEEEEVALRSDSEGSDYTPGKKKKKKLGPKKEKKSKSKRKEEEEEEDDDDDSKEPKSSAQLLEDWGMEDIDHVFSEEDYRTLTNYKAFSQFVRPLIAAKNPKIAVSKMMMVLGAKWREFSTNNPFKGSSGASVAAAAAAAVAVVESMVTATEVAPPPPPVEVPIRKAKTKEGKGPNARRKPKGSPRVPDAKKPKPKKVAPLKIKLGGFGSKRKRSSSEDDDLDVESDFDDASINSYSVSDGSTSRSSRSRKKLRTTKKKKKGEEEVTAVDGYETDHQDYCEVCQQGGEIILCDTCPRAYHMVCLDPDMEKAPEGKWSCPHCEKEGIQWEAKEDNSEGEEILEEVGGDLEEEDDHHMEFCRVCKDGGELLCCDTCPSSYHIHCLNPPLPEIPNGEWLCPRCTCPALKGKVQKILIWKWGQPPSPTPVPRPPDADPNTPSPKPLEGRPERQFFVKWQGMSYWHCSWVSELQLELHCQVMFRNYQRKNDMDEPPSGDFGGDEEKSRKRKNKDPKFAEMEERFYRYGIKPEWMMIHRILNHSVDKKGHVHYLIKWRDLPYDQASWESEDVEIQDYDLFKQSYWNHRELMRGEEGRPGKKLKKVKLRKLERPPETPTVDPTVKYERQPEYLDATGGTLHPYQMEGLNWLRFSWAQGTDTILADEMGLGKTVQTAVFLYSLYKEGHSKGPFLVSAPLSTIINWEREFEMWAPDMYVVTYVGDKDSRAIIRENEFSFEDNAIRGGKKASRMKKEASVKFHVLLTSYELITIDMAILGSIDWACLIVDEAHRLKNNQSKFFRVLNGYSLQHKLLLTGTPLQNNLEELFHLLNFLTPERFHNLEGFLEEFADIAKEDQIKKLHDMLGPHMLRRLKADVFKNMPSKTELIVRVELSPMQKKYYKYILTRNFEALNARGGGNQVSLLNVVMDLKKCCNHPYLFPVAAMEAPKMPNGMYDGSALIRASGKLLLLQKMLKNLKEGGHRVLIFSQMTKMLDLLEDFLEHEGYKYERIDGGITGNMRQEAIDRFNAPGAQQFCFLLSTRAGGLGINLATADTVIIYDSDWNPHNDIQAFSRAHRIGQNKKVMIYRFVTRASVEERITQVAKKKMMLTHLVVRPGLGSKTGSMSKQELDDILKFGTEELFKDEATDGGGDNKEGEDSSVIHYDDKAIERLLDRNQDETEDTELQGMNEYLSSFKVAQYVVREEEMGEEEEVEREIIKQEESVDPDYWEKLLRHHYEQQQEDLARNLGKGKRIRKQVNYNDGSQEDRDWQDDQSDNQSDYSVASEEGDEDFDERSEAPRRPSRKGLRNDKDKPLPPLLARVGGNIEVLGFNARQRKAFLNAIMRYGMPPQDAFTTQWLVRDLRGKSEKEFKAYVSLFMRHLCEPGADGAETFADGVPREGLSRQHVLTRIGVMSLIRKKVQEFEHVNGRWSMPELAEVEENKKMSQPGSPSPKTPTPSTPGDTQPNTPAPVPPAEDGIKIEENSLKEEESIEGEKEVKSTAPETAIECTQAPAPASEDEKVVVEPPEGEEKVEKAEVKERTEEPMETEPKGAADVEKVEEKSAIDLTPIVVEDKEEKKEEEEKKEVMLQNGETPKDLNDEKQKKNIKQRFMFNIADGGFTELHSLWQNEERAATVTKKTYEIWHRRHDYWLLAGIINHGYARWQDIQNDPRYAILNEPFKGEMNRGNFLEIKNKFLARRFKLLEQALVIEEQLRRAAYLNMSEDPSHPSMALNTRFAEVECLAESHQHLSKESMAGNKPANAVLHKVLKQLEELLSDMKADVTRLPATIARIPPVAVRLQMSERNILSRLANRAPEPTPQQVAQQQ

Target ORF information:

RefSeq Version	NM_001273.4
Organism	Homo sapiens(human)
Definition	Homo sapiens chromodomain helicase DNA binding protein 4 (CHD4), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273.4

ORF Insert Sequence:

ATGGCGTCGG GCCTGGGCTC CCCGTCCCCC TGCTCGGCGG GCAGTGAGGA GGAGGATATG 
GATGCACTTT TGAACAACAG CCTGCCCCCA CCCCACCCAG AAAATGAAGA GGACCCAGAA 
GAGGATTTGT CAGAAACAGA GACTCCAAAG CTCAAGAAGA AGAAAAAGCC TAAGAAACCT 
CGGGACCCTA AAATCCCTAA GAGCAAGCGC CAAAAAAAGG AGCGTATGCT CTTATGCCGG 
CAGCTGGGGG ACAGCTCTGG GGAGGGGCCA GAGTTTGTGG AGGAGGAGGA AGAGGTGGCT 
CTGCGCTCAG ACAGTGAGGG CAGCGACTAT ACTCCTGGCA AGAAGAAGAA GAAGAAGCTT 
GGACCTAAGA AAGAGAAGAA GAGCAAATCC AAGCGGAAGG AGGAGGAGGA GGAGGAGGAT 
GATGATGATG ATTCAAAGGA GCCTAAATCA TCTGCTCAGC TCCTGGAAGA CTGGGGCATG 
GAAGACATTG ACCACGTGTT CTCAGAGGAG GATTATCGAA CCCTCACCAA CTACAAGGCC 
TTCAGCCAGT TTGTCAGACC CCTCATTGCT GCCAAAAATC CCAAGATTGC TGTCTCCAAG 
ATGATGATGG TTTTGGGTGC AAAATGGCGG GAGTTCAGTA CCAATAACCC CTTCAAAGGC 
AGTTCTGGGG CATCAGTGGC AGCTGCGGCA GCAGCAGCGG TAGCTGTGGT GGAGAGCATG 
GTGACAGCCA CTGAGGTTGC ACCACCACCT CCCCCTGTGG AGGTGCCTAT CCGCAAGGCC 
AAGACCAAGG AGGGCAAAGG TCCCAATGCT CGGAGGAAGC CCAAGGGCAG CCCTCGTGTA 
CCTGATGCCA AGAAGCCTAA ACCCAAGAAA GTAGCTCCCC TGAAAATCAA GCTGGGAGGT 
TTTGGTTCCA AGCGTAAGAG ATCCTCGAGT GAGGATGATG ACTTAGATGT GGAATCTGAC 
TTCGATGATG CCAGTATCAA TAGCTATTCT GTTTCTGATG GTTCCACCAG CCGTAGTAGC 
CGCAGCCGCA AGAAACTCCG AACCACTAAA AAGAAAAAGA AAGGCGAGGA GGAGGTGACT 
GCTGTGGATG GTTATGAGAC AGACCACCAG GACTATTGCG AGGTGTGCCA GCAAGGCGGT 
GAGATCATCC TGTGTGATAC CTGTCCCCGT GCTTACCACA TGGTCTGCCT GGATCCCGAC 
ATGGAGAAGG CTCCCGAGGG CAAGTGGAGC TGCCCACACT GCGAGAAGGA AGGCATCCAG 
TGGGAAGCTA AAGAGGACAA TTCGGAGGGT GAGGAGATCC TGGAAGAGGT TGGGGGAGAC 
CTCGAAGAGG AGGATGACCA CCATATGGAA TTCTGTCGGG TCTGCAAGGA TGGTGGGGAA 
CTGCTCTGCT GTGATACCTG TCCTTCTTCC TACCACATCC ACTGCCTGAA TCCCCCACTT 
CCAGAGATCC CCAACGGTGA ATGGCTCTGT CCCCGTTGTA CGTGTCCAGC TCTGAAGGGC 
AAAGTGCAGA AGATCCTAAT CTGGAAGTGG GGTCAGCCAC CATCTCCCAC ACCAGTGCCT 
CGGCCTCCAG ATGCTGATCC CAACACGCCC TCCCCAAAGC CCTTGGAGGG GCGGCCAGAG 
CGGCAGTTCT TTGTGAAATG GCAAGGCATG TCTTACTGGC ACTGCTCCTG GGTTTCTGAA 
CTGCAGCTGG AGCTGCACTG TCAGGTGATG TTCCGAAACT ATCAGCGGAA GAATGATATG 
GATGAGCCAC CTTCTGGGGA CTTTGGTGGT GATGAAGAGA AAAGCCGAAA GCGAAAGAAC 
AAGGACCCTA AATTTGCAGA GATGGAGGAA CGCTTCTATC GCTATGGGAT AAAACCCGAG 
TGGATGATGA TCCACCGAAT CCTCAACCAC AGTGTGGACA AGAAGGGCCA CGTCCACTAC 
TTGATCAAGT GGCGGGACTT ACCTTACGAT CAGGCTTCTT GGGAGAGTGA GGATGTGGAG 
ATCCAGGATT ACGACCTGTT CAAGCAGAGC TATTGGAATC ACAGGGAGTT AATGAGGGGT 
GAGGAAGGCC GACCAGGCAA GAAGCTCAAG AAGGTGAAGC TTCGGAAGTT GGAGAGGCCT 
CCAGAAACGC CAACAGTTGA TCCAACAGTG AAGTATGAGC GACAGCCAGA GTACCTGGAT 
GCTACAGGTG GAACCCTGCA CCCCTATCAA ATGGAGGGCC TGAATTGGTT GCGCTTCTCC 
TGGGCTCAGG GCACTGACAC CATCTTGGCT GATGAGATGG GCCTTGGGAA AACTGTACAG 
ACAGCAGTCT TCCTGTATTC CCTTTACAAG GAGGGTCATT CCAAAGGCCC CTTCCTAGTG 
AGCGCCCCTC TTTCTACCAT CATCAACTGG GAGCGGGAGT TTGAAATGTG GGCTCCAGAC 
ATGTATGTCG TAACCTATGT GGGTGACAAG GACAGCCGTG CCATCATCCG AGAGAATGAG 
TTCTCCTTTG AAGACAATGC CATTCGTGGT GGCAAGAAGG CCTCCCGCAT GAAGAAAGAG 
GCATCTGTGA AATTCCATGT GCTGCTGACA TCCTATGAAT TGATCACCAT TGACATGGCT 
ATTTTGGGCT CTATTGATTG GGCCTGCCTC ATCGTGGATG AAGCCCATCG GCTGAAGAAC 
AATCAGTCTA AGTTCTTCCG GGTATTGAAT GGTTACTCAC TCCAGCACAA GCTGTTGCTG 
ACTGGGACAC CATTACAAAA CAATCTGGAA GAGTTGTTTC ATCTGCTCAA CTTTCTCACC 
CCCGAGAGGT TCCACAATTT GGAAGGTTTT TTGGAGGAGT TTGCTGACAT TGCCAAGGAG 
GACCAGATAA AAAAACTGCA TGACATGCTG GGGCCGCACA TGTTGCGGCG GCTCAAAGCC 
GATGTGTTCA AGAACATGCC CTCCAAGACA GAACTAATTG TGCGTGTGGA GCTGAGCCCT 
ATGCAGAAGA AATACTACAA GTACATCCTC ACTCGAAATT TTGAAGCACT CAATGCCCGA 
GGTGGTGGCA ACCAGGTGTC TCTGCTGAAT GTGGTGATGG ATCTTAAGAA GTGCTGCAAC 
CATCCATACC TCTTCCCTGT GGCTGCAATG GAAGCTCCTA AGATGCCTAA TGGCATGTAT 
GATGGCAGTG CCCTAATCAG AGCATCTGGG AAATTATTGC TGCTGCAGAA AATGCTCAAG 
AACCTTAAGG AGGGTGGGCA TCGTGTACTC ATCTTTTCCC AGATGACCAA GATGCTAGAC 
CTGCTAGAGG ATTTCTTGGA ACATGAAGGT TATAAATACG AACGCATCGA TGGTGGAATC 
ACTGGGAACA TGCGGCAAGA GGCCATTGAC CGCTTCAATG CACCGGGTGC TCAGCAGTTC 
TGCTTCTTGC TTTCCACTCG AGCTGGGGGC CTTGGAATCA ATCTGGCCAC TGCTGACACA 
GTTATTATCT ATGACTCTGA CTGGAACCCC CATAATGACA TTCAGGCCTT TAGCAGAGCT 
CACCGGATTG GGCAAAATAA AAAGGTAATG ATCTACCGGT TTGTGACCCG TGCGTCAGTG 
GAGGAGCGCA TCACGCAGGT GGCAAAGAAG AAAATGATGC TGACGCATCT AGTGGTGCGG 
CCTGGGCTGG GCTCCAAGAC TGGATCTATG TCCAAACAGG AGCTTGATGA TATCCTCAAA 
TTTGGCACTG AGGAACTATT CAAGGATGAA GCCACTGATG GAGGAGGAGA CAACAAAGAG 
GGAGAAGATA GCAGTGTTAT CCACTACGAT GATAAGGCCA TTGAACGGCT GCTAGACCGT 
AACCAGGATG AGACTGAAGA CACAGAATTG CAGGGCATGA ATGAATATTT GAGCTCATTC 
AAAGTGGCCC AGTATGTGGT ACGGGAAGAA GAAATGGGGG AGGAAGAGGA GGTAGAACGG 
GAAATCATTA AACAGGAAGA AAGTGTGGAT CCTGACTACT GGGAGAAATT GCTGCGGCAC 
CATTATGAGC AGCAGCAAGA AGATCTAGCC CGAAATCTGG GCAAAGGAAA AAGAATCCGT 
AAACAGGTCA ACTACAATGA TGGCTCCCAG GAGGACCGAG ATTGGCAGGA CGACCAGTCC 
GACAACCAGT CCGATTACTC AGTGGCTTCA GAGGAAGGTG ATGAAGACTT TGATGAACGT 
TCAGAAGCTC CCCGTAGGCC CAGTCGTAAG GGCCTGCGGA ATGATAAAGA TAAGCCATTG 
CCTCCTCTGT TGGCCCGTGT TGGTGGGAAT ATTGAAGTAC TTGGTTTTAA TGCTCGTCAG 
CGAAAAGCCT TTCTTAATGC AATTATGCGA TATGGTATGC CACCTCAGGA TGCTTTTACT 
ACCCAGTGGC TTGTAAGAGA CCTGCGAGGC AAATCAGAGA AAGAGTTCAA GGCATATGTC 
TCTCTTTTCA TGCGGCATTT ATGTGAGCCG GGGGCAGATG GGGCTGAGAC CTTTGCTGAT 
GGTGTCCCCC GAGAAGGCCT GTCTCGCCAG CATGTCCTTA CTAGAATTGG TGTTATGTCT 
TTGATTCGCA AGAAGGTTCA GGAGTTTGAA CATGTTAATG GGCGCTGGAG CATGCCTGAA 
CTGGCTGAGG TGGAGGAAAA CAAGAAGATG TCCCAGCCAG GGTCACCCTC CCCAAAAACT 
CCTACACCCT CCACTCCAGG GGACACGCAG CCCAACACTC CTGCACCTGT CCCACCTGCT 
GAAGATGGGA TAAAAATAGA GGAAAATAGC CTCAAAGAAG AAGAGAGCAT AGAAGGAGAA 
AAGGAGGTTA AATCTACAGC CCCTGAGACT GCCATTGAGT GTACACAGGC CCCTGCCCCT 
GCCTCAGAGG ATGAAAAGGT CGTTGTTGAA CCCCCTGAGG GAGAGGAGAA AGTGGAAAAG 
GCAGAGGTGA AGGAGAGAAC AGAGGAACCT ATGGAGACAG AGCCCAAAGG TGCTGCTGAT 
GTAGAGAAGG TGGAGGAAAA GTCAGCAATA GATCTGACCC CTATTGTGGT AGAAGACAAA 
GAAGAGAAGA AAGAAGAAGA AGAGAAAAAA GAGGTGATGC TTCAGAATGG AGAGACCCCC 
AAGGACCTGA ATGATGAGAA ACAGAAGAAA AATATTAAAC AACGTTTCAT GTTTAACATT 
GCAGATGGTG GTTTTACTGA GTTGCACTCC CTTTGGCAGA ATGAAGAGCG GGCAGCCACA 
GTTACCAAGA AGACTTATGA GATCTGGCAT CGACGGCATG ACTACTGGCT GCTAGCCGGC 
ATTATAAACC ATGGCTATGC CCGGTGGCAA GACATCCAGA ATGACCCACG CTATGCCATC 
CTCAATGAGC CTTTCAAGGG TGAAATGAAC CGTGGCAATT TCTTAGAGAT CAAGAATAAA 
TTTCTAGCTC GAAGGTTTAA GCTCTTAGAA CAAGCTCTGG TGATTGAGGA ACAGCTGCGC 
CGGGCTGCTT ACTTGAACAT GTCAGAAGAC CCTTCTCACC CTTCCATGGC CCTCAACACC 
CGCTTTGCTG AGGTGGAGTG TTTGGCGGAA AGTCATCAGC ACCTGTCCAA GGAGTCAATG 
GCAGGAAACA AGCCAGCCAA TGCAGTCCTG CACAAAGTTC TGAAACAGCT GGAAGAACTG 
CTGAGTGACA TGAAAGCTGA TGTGACTCGA CTCCCAGCTA CCATTGCCCG AATTCCCCCA 
GTTGCTGTGA GGTTACAGAT GTCAGAGCGT AACATTCTCA GCCGCCTGGC AAACCGGGCA 
CCCGAACCTA CCCCACAGCA GGTAGCCCAG CAGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHD4 ( NM_001273.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_001264.2 Homo sapiens chromodomain helicase DNA binding protein 4 (CHD4), transcript variant 1, mRNA.

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CloneID	OHu28780
Clone ID Related Accession (Same CDS sequence)	NM_001273.4 , NM_001273.5 , NM_001273.3
Accession Version	NM_001273.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5739bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-03
Organism	Homo sapiens(human)
Product	chromodomain-helicase-DNA-binding protein 4 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP234473.1, X86691.1, AC006064.10 and BC038596.2. This sequence is a reference standard in the RefSeqGene project. On Jun 2, 2019 this sequence version replaced NM_001273.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.127809.1, SRR1803615.262254.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on manual assertion, conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGTCGG GCCTGGGCTC CCCGTCCCCC TGCTCGGCGG GCAGTGAGGA GGAGGATATG 
GATGCACTTT TGAACAACAG CCTGCCCCCA CCCCACCCAG AAAATGAAGA GGACCCAGAA 
GAGGATTTGT CAGAAACAGA GACTCCAAAG CTCAAGAAGA AGAAAAAGCC TAAGAAACCT 
CGGGACCCTA AAATCCCTAA GAGCAAGCGC CAAAAAAAGG AGCGTATGCT CTTATGCCGG 
CAGCTGGGGG ACAGCTCTGG GGAGGGGCCA GAGTTTGTGG AGGAGGAGGA AGAGGTGGCT 
CTGCGCTCAG ACAGTGAGGG CAGCGACTAT ACTCCTGGCA AGAAGAAGAA GAAGAAGCTT 
GGACCTAAGA AAGAGAAGAA GAGCAAATCC AAGCGGAAGG AGGAGGAGGA GGAGGAGGAT 
GATGATGATG ATTCAAAGGA GCCTAAATCA TCTGCTCAGC TCCTGGAAGA CTGGGGCATG 
GAAGACATTG ACCACGTGTT CTCAGAGGAG GATTATCGAA CCCTCACCAA CTACAAGGCC 
TTCAGCCAGT TTGTCAGACC CCTCATTGCT GCCAAAAATC CCAAGATTGC TGTCTCCAAG 
ATGATGATGG TTTTGGGTGC AAAATGGCGG GAGTTCAGTA CCAATAACCC CTTCAAAGGC 
AGTTCTGGGG CATCAGTGGC AGCTGCGGCA GCAGCAGCGG TAGCTGTGGT GGAGAGCATG 
GTGACAGCCA CTGAGGTTGC ACCACCACCT CCCCCTGTGG AGGTGCCTAT CCGCAAGGCC 
AAGACCAAGG AGGGCAAAGG TCCCAATGCT CGGAGGAAGC CCAAGGGCAG CCCTCGTGTA 
CCTGATGCCA AGAAGCCTAA ACCCAAGAAA GTAGCTCCCC TGAAAATCAA GCTGGGAGGT 
TTTGGTTCCA AGCGTAAGAG ATCCTCGAGT GAGGATGATG ACTTAGATGT GGAATCTGAC 
TTCGATGATG CCAGTATCAA TAGCTATTCT GTTTCTGATG GTTCCACCAG CCGTAGTAGC 
CGCAGCCGCA AGAAACTCCG AACCACTAAA AAGAAAAAGA AAGGCGAGGA GGAGGTGACT 
GCTGTGGATG GTTATGAGAC AGACCACCAG GACTATTGCG AGGTGTGCCA GCAAGGCGGT 
GAGATCATCC TGTGTGATAC CTGTCCCCGT GCTTACCACA TGGTCTGCCT GGATCCCGAC 
ATGGAGAAGG CTCCCGAGGG CAAGTGGAGC TGCCCACACT GCGAGAAGGA AGGCATCCAG 
TGGGAAGCTA AAGAGGACAA TTCGGAGGGT GAGGAGATCC TGGAAGAGGT TGGGGGAGAC 
CTCGAAGAGG AGGATGACCA CCATATGGAA TTCTGTCGGG TCTGCAAGGA TGGTGGGGAA 
CTGCTCTGCT GTGATACCTG TCCTTCTTCC TACCACATCC ACTGCCTGAA TCCCCCACTT 
CCAGAGATCC CCAACGGTGA ATGGCTCTGT CCCCGTTGTA CGTGTCCAGC TCTGAAGGGC 
AAAGTGCAGA AGATCCTAAT CTGGAAGTGG GGTCAGCCAC CATCTCCCAC ACCAGTGCCT 
CGGCCTCCAG ATGCTGATCC CAACACGCCC TCCCCAAAGC CCTTGGAGGG GCGGCCAGAG 
CGGCAGTTCT TTGTGAAATG GCAAGGCATG TCTTACTGGC ACTGCTCCTG GGTTTCTGAA 
CTGCAGCTGG AGCTGCACTG TCAGGTGATG TTCCGAAACT ATCAGCGGAA GAATGATATG 
GATGAGCCAC CTTCTGGGGA CTTTGGTGGT GATGAAGAGA AAAGCCGAAA GCGAAAGAAC 
AAGGACCCTA AATTTGCAGA GATGGAGGAA CGCTTCTATC GCTATGGGAT AAAACCCGAG 
TGGATGATGA TCCACCGAAT CCTCAACCAC AGTGTGGACA AGAAGGGCCA CGTCCACTAC 
TTGATCAAGT GGCGGGACTT ACCTTACGAT CAGGCTTCTT GGGAGAGTGA GGATGTGGAG 
ATCCAGGATT ACGACCTGTT CAAGCAGAGC TATTGGAATC ACAGGGAGTT AATGAGGGGT 
GAGGAAGGCC GACCAGGCAA GAAGCTCAAG AAGGTGAAGC TTCGGAAGTT GGAGAGGCCT 
CCAGAAACGC CAACAGTTGA TCCAACAGTG AAGTATGAGC GACAGCCAGA GTACCTGGAT 
GCTACAGGTG GAACCCTGCA CCCCTATCAA ATGGAGGGCC TGAATTGGTT GCGCTTCTCC 
TGGGCTCAGG GCACTGACAC CATCTTGGCT GATGAGATGG GCCTTGGGAA AACTGTACAG 
ACAGCAGTCT TCCTGTATTC CCTTTACAAG GAGGGTCATT CCAAAGGCCC CTTCCTAGTG 
AGCGCCCCTC TTTCTACCAT CATCAACTGG GAGCGGGAGT TTGAAATGTG GGCTCCAGAC 
ATGTATGTCG TAACCTATGT GGGTGACAAG GACAGCCGTG CCATCATCCG AGAGAATGAG 
TTCTCCTTTG AAGACAATGC CATTCGTGGT GGCAAGAAGG CCTCCCGCAT GAAGAAAGAG 
GCATCTGTGA AATTCCATGT GCTGCTGACA TCCTATGAAT TGATCACCAT TGACATGGCT 
ATTTTGGGCT CTATTGATTG GGCCTGCCTC ATCGTGGATG AAGCCCATCG GCTGAAGAAC 
AATCAGTCTA AGTTCTTCCG GGTATTGAAT GGTTACTCAC TCCAGCACAA GCTGTTGCTG 
ACTGGGACAC CATTACAAAA CAATCTGGAA GAGTTGTTTC ATCTGCTCAA CTTTCTCACC 
CCCGAGAGGT TCCACAATTT GGAAGGTTTT TTGGAGGAGT TTGCTGACAT TGCCAAGGAG 
GACCAGATAA AAAAACTGCA TGACATGCTG GGGCCGCACA TGTTGCGGCG GCTCAAAGCC 
GATGTGTTCA AGAACATGCC CTCCAAGACA GAACTAATTG TGCGTGTGGA GCTGAGCCCT 
ATGCAGAAGA AATACTACAA GTACATCCTC ACTCGAAATT TTGAAGCACT CAATGCCCGA 
GGTGGTGGCA ACCAGGTGTC TCTGCTGAAT GTGGTGATGG ATCTTAAGAA GTGCTGCAAC 
CATCCATACC TCTTCCCTGT GGCTGCAATG GAAGCTCCTA AGATGCCTAA TGGCATGTAT 
GATGGCAGTG CCCTAATCAG AGCATCTGGG AAATTATTGC TGCTGCAGAA AATGCTCAAG 
AACCTTAAGG AGGGTGGGCA TCGTGTACTC ATCTTTTCCC AGATGACCAA GATGCTAGAC 
CTGCTAGAGG ATTTCTTGGA ACATGAAGGT TATAAATACG AACGCATCGA TGGTGGAATC 
ACTGGGAACA TGCGGCAAGA GGCCATTGAC CGCTTCAATG CACCGGGTGC TCAGCAGTTC 
TGCTTCTTGC TTTCCACTCG AGCTGGGGGC CTTGGAATCA ATCTGGCCAC TGCTGACACA 
GTTATTATCT ATGACTCTGA CTGGAACCCC CATAATGACA TTCAGGCCTT TAGCAGAGCT 
CACCGGATTG GGCAAAATAA AAAGGTAATG ATCTACCGGT TTGTGACCCG TGCGTCAGTG 
GAGGAGCGCA TCACGCAGGT GGCAAAGAAG AAAATGATGC TGACGCATCT AGTGGTGCGG 
CCTGGGCTGG GCTCCAAGAC TGGATCTATG TCCAAACAGG AGCTTGATGA TATCCTCAAA 
TTTGGCACTG AGGAACTATT CAAGGATGAA GCCACTGATG GAGGAGGAGA CAACAAAGAG 
GGAGAAGATA GCAGTGTTAT CCACTACGAT GATAAGGCCA TTGAACGGCT GCTAGACCGT 
AACCAGGATG AGACTGAAGA CACAGAATTG CAGGGCATGA ATGAATATTT GAGCTCATTC 
AAAGTGGCCC AGTATGTGGT ACGGGAAGAA GAAATGGGGG AGGAAGAGGA GGTAGAACGG 
GAAATCATTA AACAGGAAGA AAGTGTGGAT CCTGACTACT GGGAGAAATT GCTGCGGCAC 
CATTATGAGC AGCAGCAAGA AGATCTAGCC CGAAATCTGG GCAAAGGAAA AAGAATCCGT 
AAACAGGTCA ACTACAATGA TGGCTCCCAG GAGGACCGAG ATTGGCAGGA CGACCAGTCC 
GACAACCAGT CCGATTACTC AGTGGCTTCA GAGGAAGGTG ATGAAGACTT TGATGAACGT 
TCAGAAGCTC CCCGTAGGCC CAGTCGTAAG GGCCTGCGGA ATGATAAAGA TAAGCCATTG 
CCTCCTCTGT TGGCCCGTGT TGGTGGGAAT ATTGAAGTAC TTGGTTTTAA TGCTCGTCAG 
CGAAAAGCCT TTCTTAATGC AATTATGCGA TATGGTATGC CACCTCAGGA TGCTTTTACT 
ACCCAGTGGC TTGTAAGAGA CCTGCGAGGC AAATCAGAGA AAGAGTTCAA GGCATATGTC 
TCTCTTTTCA TGCGGCATTT ATGTGAGCCG GGGGCAGATG GGGCTGAGAC CTTTGCTGAT 
GGTGTCCCCC GAGAAGGCCT GTCTCGCCAG CATGTCCTTA CTAGAATTGG TGTTATGTCT 
TTGATTCGCA AGAAGGTTCA GGAGTTTGAA CATGTTAATG GGCGCTGGAG CATGCCTGAA 
CTGGCTGAGG TGGAGGAAAA CAAGAAGATG TCCCAGCCAG GGTCACCCTC CCCAAAAACT 
CCTACACCCT CCACTCCAGG GGACACGCAG CCCAACACTC CTGCACCTGT CCCACCTGCT 
GAAGATGGGA TAAAAATAGA GGAAAATAGC CTCAAAGAAG AAGAGAGCAT AGAAGGAGAA 
AAGGAGGTTA AATCTACAGC CCCTGAGACT GCCATTGAGT GTACACAGGC CCCTGCCCCT 
GCCTCAGAGG ATGAAAAGGT CGTTGTTGAA CCCCCTGAGG GAGAGGAGAA AGTGGAAAAG 
GCAGAGGTGA AGGAGAGAAC AGAGGAACCT ATGGAGACAG AGCCCAAAGG TGCTGCTGAT 
GTAGAGAAGG TGGAGGAAAA GTCAGCAATA GATCTGACCC CTATTGTGGT AGAAGACAAA 
GAAGAGAAGA AAGAAGAAGA AGAGAAAAAA GAGGTGATGC TTCAGAATGG AGAGACCCCC 
AAGGACCTGA ATGATGAGAA ACAGAAGAAA AATATTAAAC AACGTTTCAT GTTTAACATT 
GCAGATGGTG GTTTTACTGA GTTGCACTCC CTTTGGCAGA ATGAAGAGCG GGCAGCCACA 
GTTACCAAGA AGACTTATGA GATCTGGCAT CGACGGCATG ACTACTGGCT GCTAGCCGGC 
ATTATAAACC ATGGCTATGC CCGGTGGCAA GACATCCAGA ATGACCCACG CTATGCCATC 
CTCAATGAGC CTTTCAAGGG TGAAATGAAC CGTGGCAATT TCTTAGAGAT CAAGAATAAA 
TTTCTAGCTC GAAGGTTTAA GCTCTTAGAA CAAGCTCTGG TGATTGAGGA ACAGCTGCGC 
CGGGCTGCTT ACTTGAACAT GTCAGAAGAC CCTTCTCACC CTTCCATGGC CCTCAACACC 
CGCTTTGCTG AGGTGGAGTG TTTGGCGGAA AGTCATCAGC ACCTGTCCAA GGAGTCAATG 
GCAGGAAACA AGCCAGCCAA TGCAGTCCTG CACAAAGTTC TGAAACAGCT GGAAGAACTG 
CTGAGTGACA TGAAAGCTGA TGTGACTCGA CTCCCAGCTA CCATTGCCCG AATTCCCCCA 
GTTGCTGTGA GGTTACAGAT GTCAGAGCGT AACATTCTCA GCCGCCTGGC AAACCGGGCA 
CCCGAACCTA CCCCACAGCA GGTAGCCCAG CAGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001264.2
CDS	159..5897
Translation	MASGLGSPSPCSAGSEEEDMDALLNNSLPPPHPENEEDPEEDLSETETPKLKKKKKPKKPRDPKIPKSKRQKKERMLLCRQLGDSSGEGPEFVEEEEEVALRSDSEGSDYTPGKKKKKKLGPKKEKKSKSKRKEEEEEEDDDDDSKEPKSSAQLLEDWGMEDIDHVFSEEDYRTLTNYKAFSQFVRPLIAAKNPKIAVSKMMMVLGAKWREFSTNNPFKGSSGASVAAAAAAAVAVVESMVTATEVAPPPPPVEVPIRKAKTKEGKGPNARRKPKGSPRVPDAKKPKPKKVAPLKIKLGGFGSKRKRSSSEDDDLDVESDFDDASINSYSVSDGSTSRSSRSRKKLRTTKKKKKGEEEVTAVDGYETDHQDYCEVCQQGGEIILCDTCPRAYHMVCLDPDMEKAPEGKWSCPHCEKEGIQWEAKEDNSEGEEILEEVGGDLEEEDDHHMEFCRVCKDGGELLCCDTCPSSYHIHCLNPPLPEIPNGEWLCPRCTCPALKGKVQKILIWKWGQPPSPTPVPRPPDADPNTPSPKPLEGRPERQFFVKWQGMSYWHCSWVSELQLELHCQVMFRNYQRKNDMDEPPSGDFGGDEEKSRKRKNKDPKFAEMEERFYRYGIKPEWMMIHRILNHSVDKKGHVHYLIKWRDLPYDQASWESEDVEIQDYDLFKQSYWNHRELMRGEEGRPGKKLKKVKLRKLERPPETPTVDPTVKYERQPEYLDATGGTLHPYQMEGLNWLRFSWAQGTDTILADEMGLGKTVQTAVFLYSLYKEGHSKGPFLVSAPLSTIINWEREFEMWAPDMYVVTYVGDKDSRAIIRENEFSFEDNAIRGGKKASRMKKEASVKFHVLLTSYELITIDMAILGSIDWACLIVDEAHRLKNNQSKFFRVLNGYSLQHKLLLTGTPLQNNLEELFHLLNFLTPERFHNLEGFLEEFADIAKEDQIKKLHDMLGPHMLRRLKADVFKNMPSKTELIVRVELSPMQKKYYKYILTRNFEALNARGGGNQVSLLNVVMDLKKCCNHPYLFPVAAMEAPKMPNGMYDGSALIRASGKLLLLQKMLKNLKEGGHRVLIFSQMTKMLDLLEDFLEHEGYKYERIDGGITGNMRQEAIDRFNAPGAQQFCFLLSTRAGGLGINLATADTVIIYDSDWNPHNDIQAFSRAHRIGQNKKVMIYRFVTRASVEERITQVAKKKMMLTHLVVRPGLGSKTGSMSKQELDDILKFGTEELFKDEATDGGGDNKEGEDSSVIHYDDKAIERLLDRNQDETEDTELQGMNEYLSSFKVAQYVVREEEMGEEEEVEREIIKQEESVDPDYWEKLLRHHYEQQQEDLARNLGKGKRIRKQVNYNDGSQEDRDWQDDQSDNQSDYSVASEEGDEDFDERSEAPRRPSRKGLRNDKDKPLPPLLARVGGNIEVLGFNARQRKAFLNAIMRYGMPPQDAFTTQWLVRDLRGKSEKEFKAYVSLFMRHLCEPGADGAETFADGVPREGLSRQHVLTRIGVMSLIRKKVQEFEHVNGRWSMPELAEVEENKKMSQPGSPSPKTPTPSTPGDTQPNTPAPVPPAEDGIKIEENSLKEEESIEGEKEVKSTAPETAIECTQAPAPASEDEKVVVEPPEGEEKVEKAEVKERTEEPMETEPKGAADVEKVEEKSAIDLTPIVVEDKEEKKEEEEKKEVMLQNGETPKDLNDEKQKKNIKQRFMFNIADGGFTELHSLWQNEERAATVTKKTYEIWHRRHDYWLLAGIINHGYARWQDIQNDPRYAILNEPFKGEMNRGNFLEIKNKFLARRFKLLEQALVIEEQLRRAAYLNMSEDPSHPSMALNTRFAEVECLAESHQHLSKESMAGNKPANAVLHKVLKQLEELLSDMKADVTRLPATIARIPPVAVRLQMSERNILSRLANRAPEPTPQQVAQQQ

Target ORF information:

RefSeq Version	NM_001273.5
Organism	Homo sapiens(human)
Definition	Homo sapiens chromodomain helicase DNA binding protein 4 (CHD4), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273.5

ORF Insert Sequence:

ATGGCGTCGG GCCTGGGCTC CCCGTCCCCC TGCTCGGCGG GCAGTGAGGA GGAGGATATG 
GATGCACTTT TGAACAACAG CCTGCCCCCA CCCCACCCAG AAAATGAAGA GGACCCAGAA 
GAGGATTTGT CAGAAACAGA GACTCCAAAG CTCAAGAAGA AGAAAAAGCC TAAGAAACCT 
CGGGACCCTA AAATCCCTAA GAGCAAGCGC CAAAAAAAGG AGCGTATGCT CTTATGCCGG 
CAGCTGGGGG ACAGCTCTGG GGAGGGGCCA GAGTTTGTGG AGGAGGAGGA AGAGGTGGCT 
CTGCGCTCAG ACAGTGAGGG CAGCGACTAT ACTCCTGGCA AGAAGAAGAA GAAGAAGCTT 
GGACCTAAGA AAGAGAAGAA GAGCAAATCC AAGCGGAAGG AGGAGGAGGA GGAGGAGGAT 
GATGATGATG ATTCAAAGGA GCCTAAATCA TCTGCTCAGC TCCTGGAAGA CTGGGGCATG 
GAAGACATTG ACCACGTGTT CTCAGAGGAG GATTATCGAA CCCTCACCAA CTACAAGGCC 
TTCAGCCAGT TTGTCAGACC CCTCATTGCT GCCAAAAATC CCAAGATTGC TGTCTCCAAG 
ATGATGATGG TTTTGGGTGC AAAATGGCGG GAGTTCAGTA CCAATAACCC CTTCAAAGGC 
AGTTCTGGGG CATCAGTGGC AGCTGCGGCA GCAGCAGCGG TAGCTGTGGT GGAGAGCATG 
GTGACAGCCA CTGAGGTTGC ACCACCACCT CCCCCTGTGG AGGTGCCTAT CCGCAAGGCC 
AAGACCAAGG AGGGCAAAGG TCCCAATGCT CGGAGGAAGC CCAAGGGCAG CCCTCGTGTA 
CCTGATGCCA AGAAGCCTAA ACCCAAGAAA GTAGCTCCCC TGAAAATCAA GCTGGGAGGT 
TTTGGTTCCA AGCGTAAGAG ATCCTCGAGT GAGGATGATG ACTTAGATGT GGAATCTGAC 
TTCGATGATG CCAGTATCAA TAGCTATTCT GTTTCTGATG GTTCCACCAG CCGTAGTAGC 
CGCAGCCGCA AGAAACTCCG AACCACTAAA AAGAAAAAGA AAGGCGAGGA GGAGGTGACT 
GCTGTGGATG GTTATGAGAC AGACCACCAG GACTATTGCG AGGTGTGCCA GCAAGGCGGT 
GAGATCATCC TGTGTGATAC CTGTCCCCGT GCTTACCACA TGGTCTGCCT GGATCCCGAC 
ATGGAGAAGG CTCCCGAGGG CAAGTGGAGC TGCCCACACT GCGAGAAGGA AGGCATCCAG 
TGGGAAGCTA AAGAGGACAA TTCGGAGGGT GAGGAGATCC TGGAAGAGGT TGGGGGAGAC 
CTCGAAGAGG AGGATGACCA CCATATGGAA TTCTGTCGGG TCTGCAAGGA TGGTGGGGAA 
CTGCTCTGCT GTGATACCTG TCCTTCTTCC TACCACATCC ACTGCCTGAA TCCCCCACTT 
CCAGAGATCC CCAACGGTGA ATGGCTCTGT CCCCGTTGTA CGTGTCCAGC TCTGAAGGGC 
AAAGTGCAGA AGATCCTAAT CTGGAAGTGG GGTCAGCCAC CATCTCCCAC ACCAGTGCCT 
CGGCCTCCAG ATGCTGATCC CAACACGCCC TCCCCAAAGC CCTTGGAGGG GCGGCCAGAG 
CGGCAGTTCT TTGTGAAATG GCAAGGCATG TCTTACTGGC ACTGCTCCTG GGTTTCTGAA 
CTGCAGCTGG AGCTGCACTG TCAGGTGATG TTCCGAAACT ATCAGCGGAA GAATGATATG 
GATGAGCCAC CTTCTGGGGA CTTTGGTGGT GATGAAGAGA AAAGCCGAAA GCGAAAGAAC 
AAGGACCCTA AATTTGCAGA GATGGAGGAA CGCTTCTATC GCTATGGGAT AAAACCCGAG 
TGGATGATGA TCCACCGAAT CCTCAACCAC AGTGTGGACA AGAAGGGCCA CGTCCACTAC 
TTGATCAAGT GGCGGGACTT ACCTTACGAT CAGGCTTCTT GGGAGAGTGA GGATGTGGAG 
ATCCAGGATT ACGACCTGTT CAAGCAGAGC TATTGGAATC ACAGGGAGTT AATGAGGGGT 
GAGGAAGGCC GACCAGGCAA GAAGCTCAAG AAGGTGAAGC TTCGGAAGTT GGAGAGGCCT 
CCAGAAACGC CAACAGTTGA TCCAACAGTG AAGTATGAGC GACAGCCAGA GTACCTGGAT 
GCTACAGGTG GAACCCTGCA CCCCTATCAA ATGGAGGGCC TGAATTGGTT GCGCTTCTCC 
TGGGCTCAGG GCACTGACAC CATCTTGGCT GATGAGATGG GCCTTGGGAA AACTGTACAG 
ACAGCAGTCT TCCTGTATTC CCTTTACAAG GAGGGTCATT CCAAAGGCCC CTTCCTAGTG 
AGCGCCCCTC TTTCTACCAT CATCAACTGG GAGCGGGAGT TTGAAATGTG GGCTCCAGAC 
ATGTATGTCG TAACCTATGT GGGTGACAAG GACAGCCGTG CCATCATCCG AGAGAATGAG 
TTCTCCTTTG AAGACAATGC CATTCGTGGT GGCAAGAAGG CCTCCCGCAT GAAGAAAGAG 
GCATCTGTGA AATTCCATGT GCTGCTGACA TCCTATGAAT TGATCACCAT TGACATGGCT 
ATTTTGGGCT CTATTGATTG GGCCTGCCTC ATCGTGGATG AAGCCCATCG GCTGAAGAAC 
AATCAGTCTA AGTTCTTCCG GGTATTGAAT GGTTACTCAC TCCAGCACAA GCTGTTGCTG 
ACTGGGACAC CATTACAAAA CAATCTGGAA GAGTTGTTTC ATCTGCTCAA CTTTCTCACC 
CCCGAGAGGT TCCACAATTT GGAAGGTTTT TTGGAGGAGT TTGCTGACAT TGCCAAGGAG 
GACCAGATAA AAAAACTGCA TGACATGCTG GGGCCGCACA TGTTGCGGCG GCTCAAAGCC 
GATGTGTTCA AGAACATGCC CTCCAAGACA GAACTAATTG TGCGTGTGGA GCTGAGCCCT 
ATGCAGAAGA AATACTACAA GTACATCCTC ACTCGAAATT TTGAAGCACT CAATGCCCGA 
GGTGGTGGCA ACCAGGTGTC TCTGCTGAAT GTGGTGATGG ATCTTAAGAA GTGCTGCAAC 
CATCCATACC TCTTCCCTGT GGCTGCAATG GAAGCTCCTA AGATGCCTAA TGGCATGTAT 
GATGGCAGTG CCCTAATCAG AGCATCTGGG AAATTATTGC TGCTGCAGAA AATGCTCAAG 
AACCTTAAGG AGGGTGGGCA TCGTGTACTC ATCTTTTCCC AGATGACCAA GATGCTAGAC 
CTGCTAGAGG ATTTCTTGGA ACATGAAGGT TATAAATACG AACGCATCGA TGGTGGAATC 
ACTGGGAACA TGCGGCAAGA GGCCATTGAC CGCTTCAATG CACCGGGTGC TCAGCAGTTC 
TGCTTCTTGC TTTCCACTCG AGCTGGGGGC CTTGGAATCA ATCTGGCCAC TGCTGACACA 
GTTATTATCT ATGACTCTGA CTGGAACCCC CATAATGACA TTCAGGCCTT TAGCAGAGCT 
CACCGGATTG GGCAAAATAA AAAGGTAATG ATCTACCGGT TTGTGACCCG TGCGTCAGTG 
GAGGAGCGCA TCACGCAGGT GGCAAAGAAG AAAATGATGC TGACGCATCT AGTGGTGCGG 
CCTGGGCTGG GCTCCAAGAC TGGATCTATG TCCAAACAGG AGCTTGATGA TATCCTCAAA 
TTTGGCACTG AGGAACTATT CAAGGATGAA GCCACTGATG GAGGAGGAGA CAACAAAGAG 
GGAGAAGATA GCAGTGTTAT CCACTACGAT GATAAGGCCA TTGAACGGCT GCTAGACCGT 
AACCAGGATG AGACTGAAGA CACAGAATTG CAGGGCATGA ATGAATATTT GAGCTCATTC 
AAAGTGGCCC AGTATGTGGT ACGGGAAGAA GAAATGGGGG AGGAAGAGGA GGTAGAACGG 
GAAATCATTA AACAGGAAGA AAGTGTGGAT CCTGACTACT GGGAGAAATT GCTGCGGCAC 
CATTATGAGC AGCAGCAAGA AGATCTAGCC CGAAATCTGG GCAAAGGAAA AAGAATCCGT 
AAACAGGTCA ACTACAATGA TGGCTCCCAG GAGGACCGAG ATTGGCAGGA CGACCAGTCC 
GACAACCAGT CCGATTACTC AGTGGCTTCA GAGGAAGGTG ATGAAGACTT TGATGAACGT 
TCAGAAGCTC CCCGTAGGCC CAGTCGTAAG GGCCTGCGGA ATGATAAAGA TAAGCCATTG 
CCTCCTCTGT TGGCCCGTGT TGGTGGGAAT ATTGAAGTAC TTGGTTTTAA TGCTCGTCAG 
CGAAAAGCCT TTCTTAATGC AATTATGCGA TATGGTATGC CACCTCAGGA TGCTTTTACT 
ACCCAGTGGC TTGTAAGAGA CCTGCGAGGC AAATCAGAGA AAGAGTTCAA GGCATATGTC 
TCTCTTTTCA TGCGGCATTT ATGTGAGCCG GGGGCAGATG GGGCTGAGAC CTTTGCTGAT 
GGTGTCCCCC GAGAAGGCCT GTCTCGCCAG CATGTCCTTA CTAGAATTGG TGTTATGTCT 
TTGATTCGCA AGAAGGTTCA GGAGTTTGAA CATGTTAATG GGCGCTGGAG CATGCCTGAA 
CTGGCTGAGG TGGAGGAAAA CAAGAAGATG TCCCAGCCAG GGTCACCCTC CCCAAAAACT 
CCTACACCCT CCACTCCAGG GGACACGCAG CCCAACACTC CTGCACCTGT CCCACCTGCT 
GAAGATGGGA TAAAAATAGA GGAAAATAGC CTCAAAGAAG AAGAGAGCAT AGAAGGAGAA 
AAGGAGGTTA AATCTACAGC CCCTGAGACT GCCATTGAGT GTACACAGGC CCCTGCCCCT 
GCCTCAGAGG ATGAAAAGGT CGTTGTTGAA CCCCCTGAGG GAGAGGAGAA AGTGGAAAAG 
GCAGAGGTGA AGGAGAGAAC AGAGGAACCT ATGGAGACAG AGCCCAAAGG TGCTGCTGAT 
GTAGAGAAGG TGGAGGAAAA GTCAGCAATA GATCTGACCC CTATTGTGGT AGAAGACAAA 
GAAGAGAAGA AAGAAGAAGA AGAGAAAAAA GAGGTGATGC TTCAGAATGG AGAGACCCCC 
AAGGACCTGA ATGATGAGAA ACAGAAGAAA AATATTAAAC AACGTTTCAT GTTTAACATT 
GCAGATGGTG GTTTTACTGA GTTGCACTCC CTTTGGCAGA ATGAAGAGCG GGCAGCCACA 
GTTACCAAGA AGACTTATGA GATCTGGCAT CGACGGCATG ACTACTGGCT GCTAGCCGGC 
ATTATAAACC ATGGCTATGC CCGGTGGCAA GACATCCAGA ATGACCCACG CTATGCCATC 
CTCAATGAGC CTTTCAAGGG TGAAATGAAC CGTGGCAATT TCTTAGAGAT CAAGAATAAA 
TTTCTAGCTC GAAGGTTTAA GCTCTTAGAA CAAGCTCTGG TGATTGAGGA ACAGCTGCGC 
CGGGCTGCTT ACTTGAACAT GTCAGAAGAC CCTTCTCACC CTTCCATGGC CCTCAACACC 
CGCTTTGCTG AGGTGGAGTG TTTGGCGGAA AGTCATCAGC ACCTGTCCAA GGAGTCAATG 
GCAGGAAACA AGCCAGCCAA TGCAGTCCTG CACAAAGTTC TGAAACAGCT GGAAGAACTG 
CTGAGTGACA TGAAAGCTGA TGTGACTCGA CTCCCAGCTA CCATTGCCCG AATTCCCCCA 
GTTGCTGTGA GGTTACAGAT GTCAGAGCGT AACATTCTCA GCCGCCTGGC AAACCGGGCA 
CCCGAACCTA CCCCACAGCA GGTAGCCCAG CAGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHD4 ( NM_001273.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001264.2 Homo sapiens chromodomain helicase DNA binding protein 4 (CHD4), transcript variant 1, mRNA.

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CloneID	OHu28780
Clone ID Related Accession (Same CDS sequence)	NM_001273.4 , NM_001273.5 , NM_001273.3
Accession Version	NM_001273.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5739bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-27
Organism	Homo sapiens(human)
Product	chromodomain-helicase-DNA-binding protein 4 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AA099889.1, BP234473.1, X86691.1, AC006064.10 and BC038596.1. This sequence is a reference standard in the RefSeqGene project. On Jul 3, 2014 this sequence version replaced NM_001273.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X86691.1, SRR1803615.127809.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGTCGG GCCTGGGCTC CCCGTCCCCC TGCTCGGCGG GCAGTGAGGA GGAGGATATG 
GATGCACTTT TGAACAACAG CCTGCCCCCA CCCCACCCAG AAAATGAAGA GGACCCAGAA 
GAGGATTTGT CAGAAACAGA GACTCCAAAG CTCAAGAAGA AGAAAAAGCC TAAGAAACCT 
CGGGACCCTA AAATCCCTAA GAGCAAGCGC CAAAAAAAGG AGCGTATGCT CTTATGCCGG 
CAGCTGGGGG ACAGCTCTGG GGAGGGGCCA GAGTTTGTGG AGGAGGAGGA AGAGGTGGCT 
CTGCGCTCAG ACAGTGAGGG CAGCGACTAT ACTCCTGGCA AGAAGAAGAA GAAGAAGCTT 
GGACCTAAGA AAGAGAAGAA GAGCAAATCC AAGCGGAAGG AGGAGGAGGA GGAGGAGGAT 
GATGATGATG ATTCAAAGGA GCCTAAATCA TCTGCTCAGC TCCTGGAAGA CTGGGGCATG 
GAAGACATTG ACCACGTGTT CTCAGAGGAG GATTATCGAA CCCTCACCAA CTACAAGGCC 
TTCAGCCAGT TTGTCAGACC CCTCATTGCT GCCAAAAATC CCAAGATTGC TGTCTCCAAG 
ATGATGATGG TTTTGGGTGC AAAATGGCGG GAGTTCAGTA CCAATAACCC CTTCAAAGGC 
AGTTCTGGGG CATCAGTGGC AGCTGCGGCA GCAGCAGCGG TAGCTGTGGT GGAGAGCATG 
GTGACAGCCA CTGAGGTTGC ACCACCACCT CCCCCTGTGG AGGTGCCTAT CCGCAAGGCC 
AAGACCAAGG AGGGCAAAGG TCCCAATGCT CGGAGGAAGC CCAAGGGCAG CCCTCGTGTA 
CCTGATGCCA AGAAGCCTAA ACCCAAGAAA GTAGCTCCCC TGAAAATCAA GCTGGGAGGT 
TTTGGTTCCA AGCGTAAGAG ATCCTCGAGT GAGGATGATG ACTTAGATGT GGAATCTGAC 
TTCGATGATG CCAGTATCAA TAGCTATTCT GTTTCTGATG GTTCCACCAG CCGTAGTAGC 
CGCAGCCGCA AGAAACTCCG AACCACTAAA AAGAAAAAGA AAGGCGAGGA GGAGGTGACT 
GCTGTGGATG GTTATGAGAC AGACCACCAG GACTATTGCG AGGTGTGCCA GCAAGGCGGT 
GAGATCATCC TGTGTGATAC CTGTCCCCGT GCTTACCACA TGGTCTGCCT GGATCCCGAC 
ATGGAGAAGG CTCCCGAGGG CAAGTGGAGC TGCCCACACT GCGAGAAGGA AGGCATCCAG 
TGGGAAGCTA AAGAGGACAA TTCGGAGGGT GAGGAGATCC TGGAAGAGGT TGGGGGAGAC 
CTCGAAGAGG AGGATGACCA CCATATGGAA TTCTGTCGGG TCTGCAAGGA TGGTGGGGAA 
CTGCTCTGCT GTGATACCTG TCCTTCTTCC TACCACATCC ACTGCCTGAA TCCCCCACTT 
CCAGAGATCC CCAACGGTGA ATGGCTCTGT CCCCGTTGTA CGTGTCCAGC TCTGAAGGGC 
AAAGTGCAGA AGATCCTAAT CTGGAAGTGG GGTCAGCCAC CATCTCCCAC ACCAGTGCCT 
CGGCCTCCAG ATGCTGATCC CAACACGCCC TCCCCAAAGC CCTTGGAGGG GCGGCCAGAG 
CGGCAGTTCT TTGTGAAATG GCAAGGCATG TCTTACTGGC ACTGCTCCTG GGTTTCTGAA 
CTGCAGCTGG AGCTGCACTG TCAGGTGATG TTCCGAAACT ATCAGCGGAA GAATGATATG 
GATGAGCCAC CTTCTGGGGA CTTTGGTGGT GATGAAGAGA AAAGCCGAAA GCGAAAGAAC 
AAGGACCCTA AATTTGCAGA GATGGAGGAA CGCTTCTATC GCTATGGGAT AAAACCCGAG 
TGGATGATGA TCCACCGAAT CCTCAACCAC AGTGTGGACA AGAAGGGCCA CGTCCACTAC 
TTGATCAAGT GGCGGGACTT ACCTTACGAT CAGGCTTCTT GGGAGAGTGA GGATGTGGAG 
ATCCAGGATT ACGACCTGTT CAAGCAGAGC TATTGGAATC ACAGGGAGTT AATGAGGGGT 
GAGGAAGGCC GACCAGGCAA GAAGCTCAAG AAGGTGAAGC TTCGGAAGTT GGAGAGGCCT 
CCAGAAACGC CAACAGTTGA TCCAACAGTG AAGTATGAGC GACAGCCAGA GTACCTGGAT 
GCTACAGGTG GAACCCTGCA CCCCTATCAA ATGGAGGGCC TGAATTGGTT GCGCTTCTCC 
TGGGCTCAGG GCACTGACAC CATCTTGGCT GATGAGATGG GCCTTGGGAA AACTGTACAG 
ACAGCAGTCT TCCTGTATTC CCTTTACAAG GAGGGTCATT CCAAAGGCCC CTTCCTAGTG 
AGCGCCCCTC TTTCTACCAT CATCAACTGG GAGCGGGAGT TTGAAATGTG GGCTCCAGAC 
ATGTATGTCG TAACCTATGT GGGTGACAAG GACAGCCGTG CCATCATCCG AGAGAATGAG 
TTCTCCTTTG AAGACAATGC CATTCGTGGT GGCAAGAAGG CCTCCCGCAT GAAGAAAGAG 
GCATCTGTGA AATTCCATGT GCTGCTGACA TCCTATGAAT TGATCACCAT TGACATGGCT 
ATTTTGGGCT CTATTGATTG GGCCTGCCTC ATCGTGGATG AAGCCCATCG GCTGAAGAAC 
AATCAGTCTA AGTTCTTCCG GGTATTGAAT GGTTACTCAC TCCAGCACAA GCTGTTGCTG 
ACTGGGACAC CATTACAAAA CAATCTGGAA GAGTTGTTTC ATCTGCTCAA CTTTCTCACC 
CCCGAGAGGT TCCACAATTT GGAAGGTTTT TTGGAGGAGT TTGCTGACAT TGCCAAGGAG 
GACCAGATAA AAAAACTGCA TGACATGCTG GGGCCGCACA TGTTGCGGCG GCTCAAAGCC 
GATGTGTTCA AGAACATGCC CTCCAAGACA GAACTAATTG TGCGTGTGGA GCTGAGCCCT 
ATGCAGAAGA AATACTACAA GTACATCCTC ACTCGAAATT TTGAAGCACT CAATGCCCGA 
GGTGGTGGCA ACCAGGTGTC TCTGCTGAAT GTGGTGATGG ATCTTAAGAA GTGCTGCAAC 
CATCCATACC TCTTCCCTGT GGCTGCAATG GAAGCTCCTA AGATGCCTAA TGGCATGTAT 
GATGGCAGTG CCCTAATCAG AGCATCTGGG AAATTATTGC TGCTGCAGAA AATGCTCAAG 
AACCTTAAGG AGGGTGGGCA TCGTGTACTC ATCTTTTCCC AGATGACCAA GATGCTAGAC 
CTGCTAGAGG ATTTCTTGGA ACATGAAGGT TATAAATACG AACGCATCGA TGGTGGAATC 
ACTGGGAACA TGCGGCAAGA GGCCATTGAC CGCTTCAATG CACCGGGTGC TCAGCAGTTC 
TGCTTCTTGC TTTCCACTCG AGCTGGGGGC CTTGGAATCA ATCTGGCCAC TGCTGACACA 
GTTATTATCT ATGACTCTGA CTGGAACCCC CATAATGACA TTCAGGCCTT TAGCAGAGCT 
CACCGGATTG GGCAAAATAA AAAGGTAATG ATCTACCGGT TTGTGACCCG TGCGTCAGTG 
GAGGAGCGCA TCACGCAGGT GGCAAAGAAG AAAATGATGC TGACGCATCT AGTGGTGCGG 
CCTGGGCTGG GCTCCAAGAC TGGATCTATG TCCAAACAGG AGCTTGATGA TATCCTCAAA 
TTTGGCACTG AGGAACTATT CAAGGATGAA GCCACTGATG GAGGAGGAGA CAACAAAGAG 
GGAGAAGATA GCAGTGTTAT CCACTACGAT GATAAGGCCA TTGAACGGCT GCTAGACCGT 
AACCAGGATG AGACTGAAGA CACAGAATTG CAGGGCATGA ATGAATATTT GAGCTCATTC 
AAAGTGGCCC AGTATGTGGT ACGGGAAGAA GAAATGGGGG AGGAAGAGGA GGTAGAACGG 
GAAATCATTA AACAGGAAGA AAGTGTGGAT CCTGACTACT GGGAGAAATT GCTGCGGCAC 
CATTATGAGC AGCAGCAAGA AGATCTAGCC CGAAATCTGG GCAAAGGAAA AAGAATCCGT 
AAACAGGTCA ACTACAATGA TGGCTCCCAG GAGGACCGAG ATTGGCAGGA CGACCAGTCC 
GACAACCAGT CCGATTACTC AGTGGCTTCA GAGGAAGGTG ATGAAGACTT TGATGAACGT 
TCAGAAGCTC CCCGTAGGCC CAGTCGTAAG GGCCTGCGGA ATGATAAAGA TAAGCCATTG 
CCTCCTCTGT TGGCCCGTGT TGGTGGGAAT ATTGAAGTAC TTGGTTTTAA TGCTCGTCAG 
CGAAAAGCCT TTCTTAATGC AATTATGCGA TATGGTATGC CACCTCAGGA TGCTTTTACT 
ACCCAGTGGC TTGTAAGAGA CCTGCGAGGC AAATCAGAGA AAGAGTTCAA GGCATATGTC 
TCTCTTTTCA TGCGGCATTT ATGTGAGCCG GGGGCAGATG GGGCTGAGAC CTTTGCTGAT 
GGTGTCCCCC GAGAAGGCCT GTCTCGCCAG CATGTCCTTA CTAGAATTGG TGTTATGTCT 
TTGATTCGCA AGAAGGTTCA GGAGTTTGAA CATGTTAATG GGCGCTGGAG CATGCCTGAA 
CTGGCTGAGG TGGAGGAAAA CAAGAAGATG TCCCAGCCAG GGTCACCCTC CCCAAAAACT 
CCTACACCCT CCACTCCAGG GGACACGCAG CCCAACACTC CTGCACCTGT CCCACCTGCT 
GAAGATGGGA TAAAAATAGA GGAAAATAGC CTCAAAGAAG AAGAGAGCAT AGAAGGAGAA 
AAGGAGGTTA AATCTACAGC CCCTGAGACT GCCATTGAGT GTACACAGGC CCCTGCCCCT 
GCCTCAGAGG ATGAAAAGGT CGTTGTTGAA CCCCCTGAGG GAGAGGAGAA AGTGGAAAAG 
GCAGAGGTGA AGGAGAGAAC AGAGGAACCT ATGGAGACAG AGCCCAAAGG TGCTGCTGAT 
GTAGAGAAGG TGGAGGAAAA GTCAGCAATA GATCTGACCC CTATTGTGGT AGAAGACAAA 
GAAGAGAAGA AAGAAGAAGA AGAGAAAAAA GAGGTGATGC TTCAGAATGG AGAGACCCCC 
AAGGACCTGA ATGATGAGAA ACAGAAGAAA AATATTAAAC AACGTTTCAT GTTTAACATT 
GCAGATGGTG GTTTTACTGA GTTGCACTCC CTTTGGCAGA ATGAAGAGCG GGCAGCCACA 
GTTACCAAGA AGACTTATGA GATCTGGCAT CGACGGCATG ACTACTGGCT GCTAGCCGGC 
ATTATAAACC ATGGCTATGC CCGGTGGCAA GACATCCAGA ATGACCCACG CTATGCCATC 
CTCAATGAGC CTTTCAAGGG TGAAATGAAC CGTGGCAATT TCTTAGAGAT CAAGAATAAA 
TTTCTAGCTC GAAGGTTTAA GCTCTTAGAA CAAGCTCTGG TGATTGAGGA ACAGCTGCGC 
CGGGCTGCTT ACTTGAACAT GTCAGAAGAC CCTTCTCACC CTTCCATGGC CCTCAACACC 
CGCTTTGCTG AGGTGGAGTG TTTGGCGGAA AGTCATCAGC ACCTGTCCAA GGAGTCAATG 
GCAGGAAACA AGCCAGCCAA TGCAGTCCTG CACAAAGTTC TGAAACAGCT GGAAGAACTG 
CTGAGTGACA TGAAAGCTGA TGTGACTCGA CTCCCAGCTA CCATTGCCCG AATTCCCCCA 
GTTGCTGTGA GGTTACAGAT GTCAGAGCGT AACATTCTCA GCCGCCTGGC AAACCGGGCA 
CCCGAACCTA CCCCACAGCA GGTAGCCCAG CAGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001264.2
CDS	213..5951
Translation	MASGLGSPSPCSAGSEEEDMDALLNNSLPPPHPENEEDPEEDLSETETPKLKKKKKPKKPRDPKIPKSKRQKKERMLLCRQLGDSSGEGPEFVEEEEEVALRSDSEGSDYTPGKKKKKKLGPKKEKKSKSKRKEEEEEEDDDDDSKEPKSSAQLLEDWGMEDIDHVFSEEDYRTLTNYKAFSQFVRPLIAAKNPKIAVSKMMMVLGAKWREFSTNNPFKGSSGASVAAAAAAAVAVVESMVTATEVAPPPPPVEVPIRKAKTKEGKGPNARRKPKGSPRVPDAKKPKPKKVAPLKIKLGGFGSKRKRSSSEDDDLDVESDFDDASINSYSVSDGSTSRSSRSRKKLRTTKKKKKGEEEVTAVDGYETDHQDYCEVCQQGGEIILCDTCPRAYHMVCLDPDMEKAPEGKWSCPHCEKEGIQWEAKEDNSEGEEILEEVGGDLEEEDDHHMEFCRVCKDGGELLCCDTCPSSYHIHCLNPPLPEIPNGEWLCPRCTCPALKGKVQKILIWKWGQPPSPTPVPRPPDADPNTPSPKPLEGRPERQFFVKWQGMSYWHCSWVSELQLELHCQVMFRNYQRKNDMDEPPSGDFGGDEEKSRKRKNKDPKFAEMEERFYRYGIKPEWMMIHRILNHSVDKKGHVHYLIKWRDLPYDQASWESEDVEIQDYDLFKQSYWNHRELMRGEEGRPGKKLKKVKLRKLERPPETPTVDPTVKYERQPEYLDATGGTLHPYQMEGLNWLRFSWAQGTDTILADEMGLGKTVQTAVFLYSLYKEGHSKGPFLVSAPLSTIINWEREFEMWAPDMYVVTYVGDKDSRAIIRENEFSFEDNAIRGGKKASRMKKEASVKFHVLLTSYELITIDMAILGSIDWACLIVDEAHRLKNNQSKFFRVLNGYSLQHKLLLTGTPLQNNLEELFHLLNFLTPERFHNLEGFLEEFADIAKEDQIKKLHDMLGPHMLRRLKADVFKNMPSKTELIVRVELSPMQKKYYKYILTRNFEALNARGGGNQVSLLNVVMDLKKCCNHPYLFPVAAMEAPKMPNGMYDGSALIRASGKLLLLQKMLKNLKEGGHRVLIFSQMTKMLDLLEDFLEHEGYKYERIDGGITGNMRQEAIDRFNAPGAQQFCFLLSTRAGGLGINLATADTVIIYDSDWNPHNDIQAFSRAHRIGQNKKVMIYRFVTRASVEERITQVAKKKMMLTHLVVRPGLGSKTGSMSKQELDDILKFGTEELFKDEATDGGGDNKEGEDSSVIHYDDKAIERLLDRNQDETEDTELQGMNEYLSSFKVAQYVVREEEMGEEEEVEREIIKQEESVDPDYWEKLLRHHYEQQQEDLARNLGKGKRIRKQVNYNDGSQEDRDWQDDQSDNQSDYSVASEEGDEDFDERSEAPRRPSRKGLRNDKDKPLPPLLARVGGNIEVLGFNARQRKAFLNAIMRYGMPPQDAFTTQWLVRDLRGKSEKEFKAYVSLFMRHLCEPGADGAETFADGVPREGLSRQHVLTRIGVMSLIRKKVQEFEHVNGRWSMPELAEVEENKKMSQPGSPSPKTPTPSTPGDTQPNTPAPVPPAEDGIKIEENSLKEEESIEGEKEVKSTAPETAIECTQAPAPASEDEKVVVEPPEGEEKVEKAEVKERTEEPMETEPKGAADVEKVEEKSAIDLTPIVVEDKEEKKEEEEKKEVMLQNGETPKDLNDEKQKKNIKQRFMFNIADGGFTELHSLWQNEERAATVTKKTYEIWHRRHDYWLLAGIINHGYARWQDIQNDPRYAILNEPFKGEMNRGNFLEIKNKFLARRFKLLEQALVIEEQLRRAAYLNMSEDPSHPSMALNTRFAEVECLAESHQHLSKESMAGNKPANAVLHKVLKQLEELLSDMKADVTRLPATIARIPPVAVRLQMSERNILSRLANRAPEPTPQQVAQQQ

Target ORF information:

RefSeq Version	NM_001273.3
Organism	Homo sapiens(human)
Definition	Homo sapiens chromodomain helicase DNA binding protein 4 (CHD4), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273.3

ORF Insert Sequence:

ATGGCGTCGG GCCTGGGCTC CCCGTCCCCC TGCTCGGCGG GCAGTGAGGA GGAGGATATG 
GATGCACTTT TGAACAACAG CCTGCCCCCA CCCCACCCAG AAAATGAAGA GGACCCAGAA 
GAGGATTTGT CAGAAACAGA GACTCCAAAG CTCAAGAAGA AGAAAAAGCC TAAGAAACCT 
CGGGACCCTA AAATCCCTAA GAGCAAGCGC CAAAAAAAGG AGCGTATGCT CTTATGCCGG 
CAGCTGGGGG ACAGCTCTGG GGAGGGGCCA GAGTTTGTGG AGGAGGAGGA AGAGGTGGCT 
CTGCGCTCAG ACAGTGAGGG CAGCGACTAT ACTCCTGGCA AGAAGAAGAA GAAGAAGCTT 
GGACCTAAGA AAGAGAAGAA GAGCAAATCC AAGCGGAAGG AGGAGGAGGA GGAGGAGGAT 
GATGATGATG ATTCAAAGGA GCCTAAATCA TCTGCTCAGC TCCTGGAAGA CTGGGGCATG 
GAAGACATTG ACCACGTGTT CTCAGAGGAG GATTATCGAA CCCTCACCAA CTACAAGGCC 
TTCAGCCAGT TTGTCAGACC CCTCATTGCT GCCAAAAATC CCAAGATTGC TGTCTCCAAG 
ATGATGATGG TTTTGGGTGC AAAATGGCGG GAGTTCAGTA CCAATAACCC CTTCAAAGGC 
AGTTCTGGGG CATCAGTGGC AGCTGCGGCA GCAGCAGCGG TAGCTGTGGT GGAGAGCATG 
GTGACAGCCA CTGAGGTTGC ACCACCACCT CCCCCTGTGG AGGTGCCTAT CCGCAAGGCC 
AAGACCAAGG AGGGCAAAGG TCCCAATGCT CGGAGGAAGC CCAAGGGCAG CCCTCGTGTA 
CCTGATGCCA AGAAGCCTAA ACCCAAGAAA GTAGCTCCCC TGAAAATCAA GCTGGGAGGT 
TTTGGTTCCA AGCGTAAGAG ATCCTCGAGT GAGGATGATG ACTTAGATGT GGAATCTGAC 
TTCGATGATG CCAGTATCAA TAGCTATTCT GTTTCTGATG GTTCCACCAG CCGTAGTAGC 
CGCAGCCGCA AGAAACTCCG AACCACTAAA AAGAAAAAGA AAGGCGAGGA GGAGGTGACT 
GCTGTGGATG GTTATGAGAC AGACCACCAG GACTATTGCG AGGTGTGCCA GCAAGGCGGT 
GAGATCATCC TGTGTGATAC CTGTCCCCGT GCTTACCACA TGGTCTGCCT GGATCCCGAC 
ATGGAGAAGG CTCCCGAGGG CAAGTGGAGC TGCCCACACT GCGAGAAGGA AGGCATCCAG 
TGGGAAGCTA AAGAGGACAA TTCGGAGGGT GAGGAGATCC TGGAAGAGGT TGGGGGAGAC 
CTCGAAGAGG AGGATGACCA CCATATGGAA TTCTGTCGGG TCTGCAAGGA TGGTGGGGAA 
CTGCTCTGCT GTGATACCTG TCCTTCTTCC TACCACATCC ACTGCCTGAA TCCCCCACTT 
CCAGAGATCC CCAACGGTGA ATGGCTCTGT CCCCGTTGTA CGTGTCCAGC TCTGAAGGGC 
AAAGTGCAGA AGATCCTAAT CTGGAAGTGG GGTCAGCCAC CATCTCCCAC ACCAGTGCCT 
CGGCCTCCAG ATGCTGATCC CAACACGCCC TCCCCAAAGC CCTTGGAGGG GCGGCCAGAG 
CGGCAGTTCT TTGTGAAATG GCAAGGCATG TCTTACTGGC ACTGCTCCTG GGTTTCTGAA 
CTGCAGCTGG AGCTGCACTG TCAGGTGATG TTCCGAAACT ATCAGCGGAA GAATGATATG 
GATGAGCCAC CTTCTGGGGA CTTTGGTGGT GATGAAGAGA AAAGCCGAAA GCGAAAGAAC 
AAGGACCCTA AATTTGCAGA GATGGAGGAA CGCTTCTATC GCTATGGGAT AAAACCCGAG 
TGGATGATGA TCCACCGAAT CCTCAACCAC AGTGTGGACA AGAAGGGCCA CGTCCACTAC 
TTGATCAAGT GGCGGGACTT ACCTTACGAT CAGGCTTCTT GGGAGAGTGA GGATGTGGAG 
ATCCAGGATT ACGACCTGTT CAAGCAGAGC TATTGGAATC ACAGGGAGTT AATGAGGGGT 
GAGGAAGGCC GACCAGGCAA GAAGCTCAAG AAGGTGAAGC TTCGGAAGTT GGAGAGGCCT 
CCAGAAACGC CAACAGTTGA TCCAACAGTG AAGTATGAGC GACAGCCAGA GTACCTGGAT 
GCTACAGGTG GAACCCTGCA CCCCTATCAA ATGGAGGGCC TGAATTGGTT GCGCTTCTCC 
TGGGCTCAGG GCACTGACAC CATCTTGGCT GATGAGATGG GCCTTGGGAA AACTGTACAG 
ACAGCAGTCT TCCTGTATTC CCTTTACAAG GAGGGTCATT CCAAAGGCCC CTTCCTAGTG 
AGCGCCCCTC TTTCTACCAT CATCAACTGG GAGCGGGAGT TTGAAATGTG GGCTCCAGAC 
ATGTATGTCG TAACCTATGT GGGTGACAAG GACAGCCGTG CCATCATCCG AGAGAATGAG 
TTCTCCTTTG AAGACAATGC CATTCGTGGT GGCAAGAAGG CCTCCCGCAT GAAGAAAGAG 
GCATCTGTGA AATTCCATGT GCTGCTGACA TCCTATGAAT TGATCACCAT TGACATGGCT 
ATTTTGGGCT CTATTGATTG GGCCTGCCTC ATCGTGGATG AAGCCCATCG GCTGAAGAAC 
AATCAGTCTA AGTTCTTCCG GGTATTGAAT GGTTACTCAC TCCAGCACAA GCTGTTGCTG 
ACTGGGACAC CATTACAAAA CAATCTGGAA GAGTTGTTTC ATCTGCTCAA CTTTCTCACC 
CCCGAGAGGT TCCACAATTT GGAAGGTTTT TTGGAGGAGT TTGCTGACAT TGCCAAGGAG 
GACCAGATAA AAAAACTGCA TGACATGCTG GGGCCGCACA TGTTGCGGCG GCTCAAAGCC 
GATGTGTTCA AGAACATGCC CTCCAAGACA GAACTAATTG TGCGTGTGGA GCTGAGCCCT 
ATGCAGAAGA AATACTACAA GTACATCCTC ACTCGAAATT TTGAAGCACT CAATGCCCGA 
GGTGGTGGCA ACCAGGTGTC TCTGCTGAAT GTGGTGATGG ATCTTAAGAA GTGCTGCAAC 
CATCCATACC TCTTCCCTGT GGCTGCAATG GAAGCTCCTA AGATGCCTAA TGGCATGTAT 
GATGGCAGTG CCCTAATCAG AGCATCTGGG AAATTATTGC TGCTGCAGAA AATGCTCAAG 
AACCTTAAGG AGGGTGGGCA TCGTGTACTC ATCTTTTCCC AGATGACCAA GATGCTAGAC 
CTGCTAGAGG ATTTCTTGGA ACATGAAGGT TATAAATACG AACGCATCGA TGGTGGAATC 
ACTGGGAACA TGCGGCAAGA GGCCATTGAC CGCTTCAATG CACCGGGTGC TCAGCAGTTC 
TGCTTCTTGC TTTCCACTCG AGCTGGGGGC CTTGGAATCA ATCTGGCCAC TGCTGACACA 
GTTATTATCT ATGACTCTGA CTGGAACCCC CATAATGACA TTCAGGCCTT TAGCAGAGCT 
CACCGGATTG GGCAAAATAA AAAGGTAATG ATCTACCGGT TTGTGACCCG TGCGTCAGTG 
GAGGAGCGCA TCACGCAGGT GGCAAAGAAG AAAATGATGC TGACGCATCT AGTGGTGCGG 
CCTGGGCTGG GCTCCAAGAC TGGATCTATG TCCAAACAGG AGCTTGATGA TATCCTCAAA 
TTTGGCACTG AGGAACTATT CAAGGATGAA GCCACTGATG GAGGAGGAGA CAACAAAGAG 
GGAGAAGATA GCAGTGTTAT CCACTACGAT GATAAGGCCA TTGAACGGCT GCTAGACCGT 
AACCAGGATG AGACTGAAGA CACAGAATTG CAGGGCATGA ATGAATATTT GAGCTCATTC 
AAAGTGGCCC AGTATGTGGT ACGGGAAGAA GAAATGGGGG AGGAAGAGGA GGTAGAACGG 
GAAATCATTA AACAGGAAGA AAGTGTGGAT CCTGACTACT GGGAGAAATT GCTGCGGCAC 
CATTATGAGC AGCAGCAAGA AGATCTAGCC CGAAATCTGG GCAAAGGAAA AAGAATCCGT 
AAACAGGTCA ACTACAATGA TGGCTCCCAG GAGGACCGAG ATTGGCAGGA CGACCAGTCC 
GACAACCAGT CCGATTACTC AGTGGCTTCA GAGGAAGGTG ATGAAGACTT TGATGAACGT 
TCAGAAGCTC CCCGTAGGCC CAGTCGTAAG GGCCTGCGGA ATGATAAAGA TAAGCCATTG 
CCTCCTCTGT TGGCCCGTGT TGGTGGGAAT ATTGAAGTAC TTGGTTTTAA TGCTCGTCAG 
CGAAAAGCCT TTCTTAATGC AATTATGCGA TATGGTATGC CACCTCAGGA TGCTTTTACT 
ACCCAGTGGC TTGTAAGAGA CCTGCGAGGC AAATCAGAGA AAGAGTTCAA GGCATATGTC 
TCTCTTTTCA TGCGGCATTT ATGTGAGCCG GGGGCAGATG GGGCTGAGAC CTTTGCTGAT 
GGTGTCCCCC GAGAAGGCCT GTCTCGCCAG CATGTCCTTA CTAGAATTGG TGTTATGTCT 
TTGATTCGCA AGAAGGTTCA GGAGTTTGAA CATGTTAATG GGCGCTGGAG CATGCCTGAA 
CTGGCTGAGG TGGAGGAAAA CAAGAAGATG TCCCAGCCAG GGTCACCCTC CCCAAAAACT 
CCTACACCCT CCACTCCAGG GGACACGCAG CCCAACACTC CTGCACCTGT CCCACCTGCT 
GAAGATGGGA TAAAAATAGA GGAAAATAGC CTCAAAGAAG AAGAGAGCAT AGAAGGAGAA 
AAGGAGGTTA AATCTACAGC CCCTGAGACT GCCATTGAGT GTACACAGGC CCCTGCCCCT 
GCCTCAGAGG ATGAAAAGGT CGTTGTTGAA CCCCCTGAGG GAGAGGAGAA AGTGGAAAAG 
GCAGAGGTGA AGGAGAGAAC AGAGGAACCT ATGGAGACAG AGCCCAAAGG TGCTGCTGAT 
GTAGAGAAGG TGGAGGAAAA GTCAGCAATA GATCTGACCC CTATTGTGGT AGAAGACAAA 
GAAGAGAAGA AAGAAGAAGA AGAGAAAAAA GAGGTGATGC TTCAGAATGG AGAGACCCCC 
AAGGACCTGA ATGATGAGAA ACAGAAGAAA AATATTAAAC AACGTTTCAT GTTTAACATT 
GCAGATGGTG GTTTTACTGA GTTGCACTCC CTTTGGCAGA ATGAAGAGCG GGCAGCCACA 
GTTACCAAGA AGACTTATGA GATCTGGCAT CGACGGCATG ACTACTGGCT GCTAGCCGGC 
ATTATAAACC ATGGCTATGC CCGGTGGCAA GACATCCAGA ATGACCCACG CTATGCCATC 
CTCAATGAGC CTTTCAAGGG TGAAATGAAC CGTGGCAATT TCTTAGAGAT CAAGAATAAA 
TTTCTAGCTC GAAGGTTTAA GCTCTTAGAA CAAGCTCTGG TGATTGAGGA ACAGCTGCGC 
CGGGCTGCTT ACTTGAACAT GTCAGAAGAC CCTTCTCACC CTTCCATGGC CCTCAACACC 
CGCTTTGCTG AGGTGGAGTG TTTGGCGGAA AGTCATCAGC ACCTGTCCAA GGAGTCAATG 
GCAGGAAACA AGCCAGCCAA TGCAGTCCTG CACAAAGTTC TGAAACAGCT GGAAGAACTG 
CTGAGTGACA TGAAAGCTGA TGTGACTCGA CTCCCAGCTA CCATTGCCCG AATTCCCCCA 
GTTGCTGTGA GGTTACAGAT GTCAGAGCGT AACATTCTCA GCCGCCTGGC AAACCGGGCA 
CCCGAACCTA CCCCACAGCA GGTAGCCCAG CAGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHD4 ( XM_024448802.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024304570.1 Homo sapiens chromodomain helicase DNA binding protein 4 (CHD4), transcript variant X1, mRNA.

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CloneID	OHu103674
Clone ID Related Accession (Same CDS sequence)	XM_024448802.1
Accession Version	XM_024448802.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5826bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-25
Organism	Homo sapiens(human)
Product	chromodomain-helicase-DNA-binding protein 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCGG GCCTGGGCTC CCCGTCCCCC TGCTCGGCGG GCAGTGAGGA GGAGGATATG 
GATGCACTTT TGAACAACAG CCTGCCCCCA CCCCACCCAG AAAATGAAGA GGACCCAGAA 
GAGGATTTGT CAGAAACAGA GACTCCAAAG CTCAAGAAGA AGAAAAAGCC TAAGAAACCT 
CGGGACCCTA AAATCCCTAA GAGCAAGCGC CAAAAAAAGG AGCGTATGCT CTTATGCCGG 
CAGCTGGGGG ACAGCTCTGG GGAGGGGCCA GAGTTTGTGG AGGAGGAGGA AGAGGTGGCT 
CTGCGCTCAG ACAGTGAGGG CAGCGACTAT ACTCCTGGCA AGAAGAAGAA GAAGAAGCTT 
GGACCTAAGA AAGAGAAGAA GAGCAAATCC AAGCGGAAGG AGGAGGAGGA GGAGGAGGAT 
GATGATGATG ATTCAAAGGA GCCTAAATCA TCTGCTCAGC TCCTGGAAGA CTGGGGCATG 
GAAGACATTG ACCACGTGTT CTCAGAGGAG GATTATCGAA CCCTCACCAA CTACAAGGCC 
TTCAGCCAGT TTGTCAGACC CCTCATTGCT GCCAAAAATC CCAAGATTGC TGTCTCCAAG 
ATGATGATGG TTTTGGGTGC AAAATGGCGG GAGTTCAGTA CCAATAACCC CTTCAAAGGC 
AGTTCTGGGG CATCAGTGGC AGCTGCGGCA GCAGCAGCGG TAGCTGTGGT GGAGAGCATG 
GTGACAGCCA CTGAGGTTGC ACCACCACCT CCCCCTGTGG AGGTGCCTAT CCGCAAGGCC 
AAGACCAAGG AGGGCAAAGG TCCCAATGCT CGGAGGAAGC CCAAGGGCAG CCCTCGTGTA 
CCTGATGCCA AGAAGCCTAA ACCCAAGAAA GTAGCTCCCC TGAAAATCAA GCTGGGAGGT 
TTTGGTTCCA AGCGTAAGAG ATCCTCGAGT GAGGATGATG ACTTAGATGT GGAATCTGAC 
TTCGATGATG CCAGTATCAA TAGCTATTCT GTTTCTGATG GTTCCACCAG CCGTAGTAGC 
CGCAGCCGCA AGAAACTCCG AACCACTAAA AAGAAAAAGA AAGGCGAGGA GGAGGTGACT 
GCTGTGGATG GTTATGAGAC AGACCACCAG GACTATTGCG AGGTGTGCCA GCAAGGCGGT 
GAGATCATCC TGTGTGATAC CTGTCCCCGT GCTTACCACA TGGTCTGCCT GGATCCCGAC 
ATGGAGAAGG CTCCCGAGGG CAAGTGGAGC TGCCCACACT GCGAGAAGGA AGGCATCCAG 
TGGGAAGCTA AAGAGGACAA TTCGGAGGGT GAGGAGATCC TGGAAGAGGT TGGGGGAGAC 
CTCGAAGAGG AGGATGACCA CCATATGGAA TTCTGTCGGG TCTGCAAGGA TGGTGGGGAA 
CTGCTCTGCT GTGATACCTG TCCTTCTTCC TACCACATCC ACTGCCTGAA TCCCCCACTT 
CCAGAGATCC CCAACGGTGA ATGGCTCTGT CCCCGTTGTA CGTGTCCAGC TCTGAAGGGC 
AAAGTGCAGA AGATCCTAAT CTGGAAGTGG GGTCAGCCAC CATCTCCCAC ACCAGTGCCT 
CGGCCTCCAG ATGCTGATCC CAACACGCCC TCCCCAAAGC CCTTGGAGGG GCGGCCAGAG 
CGGCAGTTCT TTGTGAAATG GCAAGGCATG TCTTACTGGC ACTGCTCCTG GGTTTCTGAA 
CTGCAGCTGG AGCTGCACTG TCAGGTGATG TTCCGAAACT ATCAGCGGAA GAATGATATG 
GATGAGCCAC CTTCTGGGGA CTTTGGTGGT GATGAAGAGA AAAGCCGAAA GCGAAAGAAC 
AAGGACCCTA AATTTGCAGA GATGGAGGAA CGCTTCTATC GCTATGGGAT AAAACCCGAG 
TGGATGATGA TCCACCGAAT CCTCAACCAC AGTGTGGACA AGAAGGGCCA CGTCCACTAC 
TTGATCAAGT GGCGGGACTT ACCTTACGAT CAGGCTTCTT GGGAGAGTGA GGATGTGGAG 
ATCCAGGATT ACGACCTGTT CAAGCAGAGC TATTGGAATC ACAGGGAGTT AATGAGGGGT 
GAGGAAGGCC GACCAGGCAA GAAGCTCAAG AAGGTGAAGC TTCGGAAGTT GGAGAGGCCT 
CCAGAAACGC CAACAGTTGA TCCAACAGTG AAGTATGAGC GACAGCCAGA GTACCTGGAT 
GCTACAGGTG GAACCCTGCA CCCCTATCAA ATGGAGGGCC TGAATTGGTT GCGCTTCTCC 
TGGGCTCAGG GCACTGACAC CATCTTGGCT GATGAGATGG GCCTTGGGAA AACTGTACAG 
ACAGCAGTCT TCCTGTATTC CCTTTACAAG GAGGGTCATT CCAAAGGCCC CTTCCTAGTG 
AGCGCCCCTC TTTCTACCAT CATCAACTGG GAGCGGGAGT TTGAAATGTG GGCTCCAGAC 
ATGTATGTCG TAACCTATGT GGGTGACAAG GACAGCCGTG CCATCATCCG AGAGAATGAG 
TTCTCCTTTG AAGACAATGC CATTCGTGGT GGCAAGAAGG CCTCCCGCAT GAAGAAAGAG 
GCATCTGTGA AATTCCATGT GCTGCTGACA TCCTATGAAT TGATCACCAT TGACATGGCT 
ATTTTGGGCT CTATTGATTG GGCCTGCCTC ATCGTGGATG AAGCCCATCG GCTGAAGAAC 
AATCAGTCTA AGTTCTTCCG GGTATTGAAT GGTTACTCAC TCCAGCACAA GCTGTTGCTG 
ACTGGGACAC CATTACAAAA CAATCTGGAA GAGTTGTTTC ATCTGCTCAA CTTTCTCACC 
CCCGAGAGGT TCCACAATTT GGAAGGTTTT TTGGAGGAGT TTGCTGACAT TGCCAAGGAG 
GACCAGATAA AAAAACTGCA TGACATGCTG GGGCCGCACA TGTTGCGGCG GCTCAAAGCC 
GATGTGTTCA AGAACATGCC CTCCAAGACA GAACTAATTG TGCGTGTGGA GCTGAGCCCT 
ATGCAGAAGA AATACTACAA GTACATCCTC ACTCGAAATT TTGAAGCACT CAATGCCCGA 
GGTGGTGGCA ACCAGGTGTC TCTGCTGAAT GTGGTGATGG ATCTTAAGAA GTGCTGCAAC 
CATCCATACC TCTTCCCTGT GGCTGCAATG GAAGCTCCTA AGATGCCTAA TGGCATGTAT 
GATGGCAGTG CCCTAATCAG AGCATCTGGG AAATTATTGC TGCTGCAGAA AATGCTCAAG 
AACCTTAAGG AGGGTGGGCA TCGTGTACTC ATCTTTTCCC AGATGACCAA GATGCTAGAC 
CTGCTAGAGG ATTTCTTGGA ACATGAAGGT TATAAATACG AACGCATCGA TGGTGGAATC 
ACTGGGAACA TGCGGCAAGA GGCCATTGAC CGCTTCAATG CACCGGGTGC TCAGCAGTTC 
TGCTTCTTGC TTTCCACTCG AGCTGGGGGC CTTGGAATCA ATCTGGCCAC TGCTGACACA 
GTTATTATCT ATGACTCTGA CTGGAACCCC CATAATGACA TTCAGGCCTT TAGCAGAGCT 
CACCGGATTG GGCAAAATAA AAAGGTAATG ATCTACCGGT TTGTGACCCG TGCGTCAGTG 
GAGGAGCGCA TCACGCAGGT GGCAAAGAAG AAAATGATGC TGACGCATCT AGTGGTGCGG 
CCTGGGCTGG GCTCCAAGAC TGGATCTATG TCCAAACAGG AGCTTGATGA TATCCTCAAA 
TTTGGCACTG AGGAACTATT CAAGGATGAA GCCACTGATG GAGGAGGAGA CAACAAAGAG 
GGAGAAGATA GCAGTGTTAT CCACTACGAT GATAAGGCCA TTGAACGGCT GCTAGACCGT 
AACCAGGATG AGACTGAAGA CACAGAATTG CAGGGCATGA ATGAATATTT GAGCTCATTC 
AAAGTGGCCC AGTATGTGGT ACGGGAAGAA GAAATGGGGG AGGAAGAGGA GGTAGAACGG 
GAAATCATTA AACAGGAAGA AAGTGTGGAT CCTGACTACT GGGAGAAATT GCTGCGGCAC 
CATTATGAGC AGCAGCAAGA AGATCTAGCC CGAAATCTGG GCAAAGGAAA AAGAATCCGT 
AAACAGGTCA ACTACAATGA TGGCTCCCAG GAGGACCGAG GTGTGTGTGG CCGGCCCCGC 
CCCCCACCCA TGGGCCGTTC CACTAGAGCA GTGGGCCCCG CTCATCTGCC CTCTCTCCCT 
CCAGATTGGC AGGACGACCA GTCCGACAAC CAGTCCGATT ACTCAGTGGC TTCAGAGGAA 
GGTGATGAAG ACTTTGATGA ACGTTCAGAA GCTCCCCGTA GGCCCAGTCG TAAGGGCCTG 
CGGAATGATA AAGATAAGCC ATTGCCTCCT CTGTTGGCCC GTGTTGGTGG GAATATTGAA 
GTACTTGGTT TTAATGCTCG TCAGCGAAAA GCCTTTCTTA ATGCAATTAT GCGATATGGT 
ATGCCACCTC AGGATGCTTT TACTACCCAG TGGCTTGTAA GAGACCTGCG AGGCAAATCA 
GAGAAAGAGT TCAAGGCATA TGTCTCTCTT TTCATGCGGC ATTTATGTGA GCCGGGGGCA 
GATGGGGCTG AGACCTTTGC TGATGGTGTC CCCCGAGAAG GCCTGTCTCG CCAGCATGTC 
CTTACTAGAA TTGGTGTTAT GTCTTTGATT CGCAAGAAGG TTCAGGAGTT TGAACATGTT 
AATGGGCGCT GGAGCATGCC TGAACTGGCT GAGGTGGAGG AAAACAAGAA GATGTCCCAG 
CCAGGGTCAC CCTCCCCAAA AACTCCTACA CCCTCCACTC CAGGGGACAC GCAGCCCAAC 
ACTCCTGCAC CTGTCCCACC TGCTGAAGAT GGGATAAAAA TAGAGGAAAA TAGCCTCAAA 
GAAGAAGAGA GCATAGAAGG AGAAAAGGAG GTTAAATCTA CAGCCCCTGA GACTGCCATT 
GAGTGTACAC AGGCCCCTGC CCCTGCCTCA GAGGATGAAA AGGTCGTTGT TGAACCCCCT 
GAGGGAGAGG AGAAAGTGGA AAAGGCAGAG GTGAAGGAGA GAACAGAGGA ACCTATGGAG 
ACAGAGCCCA AAGGTGCTGC TGATGTAGAG AAGGTGGAGG AAAAGTCAGC AATAGATCTG 
ACCCCTATTG TGGTAGAAGA CAAAGAAGAG AAGAAAGAAG AAGAAGAGAA AAAAGAGGTG 
ATGCTTCAGA ATGGAGAGAC CCCCAAGGAC CTGAATGATG AGAAACAGAA GAAAAATATT 
AAACAACGTT TCATGTTTAA CATTGCAGAT GGTGGTTTTA CTGAGTTGCA CTCCCTTTGG 
CAGAATGAAG AGCGGGCAGC CACAGTTACC AAGAAGACTT ATGAGATCTG GCATCGACGG 
CATGACTACT GGCTGCTAGC CGGCATTATA AACCATGGCT ATGCCCGGTG GCAAGACATC 
CAGAATGACC CACGCTATGC CATCCTCAAT GAGCCTTTCA AGGGTGAAAT GAACCGTGGC 
AATTTCTTAG AGATCAAGAA TAAATTTCTA GCTCGAAGGT TTAAGCTCTT AGAACAAGCT 
CTGGTGATTG AGGAACAGCT GCGCCGGGCT GCTTACTTGA ACATGTCAGA AGACCCTTCT 
CACCCTTCCA TGGCCCTCAA CACCCGCTTT GCTGAGGTGG AGTGTTTGGC GGAAAGTCAT 
CAGCACCTGT CCAAGGAGTC AATGGCAGGA AACAAGCCAG CCAATGCAGT CCTGCACAAA 
GGTATTCTGA AACAGCTGGA AGAACTGCTG AGTGACATGA AAGCTGATGT GACTCGACTC 
CCAGCTACCA TTGCCCGAAT TCCCCCAGTT GCTGTGAGGT TACAGATGTC AGAGCGTAAC 
ATTCTCAGCC GCCTGGCAAA CCGGGCACCC GAACCTACCC CACAGCAGGT AGCCCAGCAG 
CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024304570.1
CDS	219..6044
Translation	MASGLGSPSPCSAGSEEEDMDALLNNSLPPPHPENEEDPEEDLSETETPKLKKKKKPKKPRDPKIPKSKRQKKERMLLCRQLGDSSGEGPEFVEEEEEVALRSDSEGSDYTPGKKKKKKLGPKKEKKSKSKRKEEEEEEDDDDDSKEPKSSAQLLEDWGMEDIDHVFSEEDYRTLTNYKAFSQFVRPLIAAKNPKIAVSKMMMVLGAKWREFSTNNPFKGSSGASVAAAAAAAVAVVESMVTATEVAPPPPPVEVPIRKAKTKEGKGPNARRKPKGSPRVPDAKKPKPKKVAPLKIKLGGFGSKRKRSSSEDDDLDVESDFDDASINSYSVSDGSTSRSSRSRKKLRTTKKKKKGEEEVTAVDGYETDHQDYCEVCQQGGEIILCDTCPRAYHMVCLDPDMEKAPEGKWSCPHCEKEGIQWEAKEDNSEGEEILEEVGGDLEEEDDHHMEFCRVCKDGGELLCCDTCPSSYHIHCLNPPLPEIPNGEWLCPRCTCPALKGKVQKILIWKWGQPPSPTPVPRPPDADPNTPSPKPLEGRPERQFFVKWQGMSYWHCSWVSELQLELHCQVMFRNYQRKNDMDEPPSGDFGGDEEKSRKRKNKDPKFAEMEERFYRYGIKPEWMMIHRILNHSVDKKGHVHYLIKWRDLPYDQASWESEDVEIQDYDLFKQSYWNHRELMRGEEGRPGKKLKKVKLRKLERPPETPTVDPTVKYERQPEYLDATGGTLHPYQMEGLNWLRFSWAQGTDTILADEMGLGKTVQTAVFLYSLYKEGHSKGPFLVSAPLSTIINWEREFEMWAPDMYVVTYVGDKDSRAIIRENEFSFEDNAIRGGKKASRMKKEASVKFHVLLTSYELITIDMAILGSIDWACLIVDEAHRLKNNQSKFFRVLNGYSLQHKLLLTGTPLQNNLEELFHLLNFLTPERFHNLEGFLEEFADIAKEDQIKKLHDMLGPHMLRRLKADVFKNMPSKTELIVRVELSPMQKKYYKYILTRNFEALNARGGGNQVSLLNVVMDLKKCCNHPYLFPVAAMEAPKMPNGMYDGSALIRASGKLLLLQKMLKNLKEGGHRVLIFSQMTKMLDLLEDFLEHEGYKYERIDGGITGNMRQEAIDRFNAPGAQQFCFLLSTRAGGLGINLATADTVIIYDSDWNPHNDIQAFSRAHRIGQNKKVMIYRFVTRASVEERITQVAKKKMMLTHLVVRPGLGSKTGSMSKQELDDILKFGTEELFKDEATDGGGDNKEGEDSSVIHYDDKAIERLLDRNQDETEDTELQGMNEYLSSFKVAQYVVREEEMGEEEEVEREIIKQEESVDPDYWEKLLRHHYEQQQEDLARNLGKGKRIRKQVNYNDGSQEDRGVCGRPRPPPMGRSTRAVGPAHLPSLPPDWQDDQSDNQSDYSVASEEGDEDFDERSEAPRRPSRKGLRNDKDKPLPPLLARVGGNIEVLGFNARQRKAFLNAIMRYGMPPQDAFTTQWLVRDLRGKSEKEFKAYVSLFMRHLCEPGADGAETFADGVPREGLSRQHVLTRIGVMSLIRKKVQEFEHVNGRWSMPELAEVEENKKMSQPGSPSPKTPTPSTPGDTQPNTPAPVPPAEDGIKIEENSLKEEESIEGEKEVKSTAPETAIECTQAPAPASEDEKVVVEPPEGEEKVEKAEVKERTEEPMETEPKGAADVEKVEEKSAIDLTPIVVEDKEEKKEEEEKKEVMLQNGETPKDLNDEKQKKNIKQRFMFNIADGGFTELHSLWQNEERAATVTKKTYEIWHRRHDYWLLAGIINHGYARWQDIQNDPRYAILNEPFKGEMNRGNFLEIKNKFLARRFKLLEQALVIEEQLRRAAYLNMSEDPSHPSMALNTRFAEVECLAESHQHLSKESMAGNKPANAVLHKGILKQLEELLSDMKADVTRLPATIARIPPVAVRLQMSERNILSRLANRAPEPTPQQVAQQQ

Target ORF information:

RefSeq Version	XM_024448802.1
Organism	Homo sapiens(human)
Definition	Homo sapiens chromodomain helicase DNA binding protein 4 (CHD4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024448802.1

ORF Insert Sequence:

ATGGCGTCGG GCCTGGGCTC CCCGTCCCCC TGCTCGGCGG GCAGTGAGGA GGAGGATATG 
GATGCACTTT TGAACAACAG CCTGCCCCCA CCCCACCCAG AAAATGAAGA GGACCCAGAA 
GAGGATTTGT CAGAAACAGA GACTCCAAAG CTCAAGAAGA AGAAAAAGCC TAAGAAACCT 
CGGGACCCTA AAATCCCTAA GAGCAAGCGC CAAAAAAAGG AGCGTATGCT CTTATGCCGG 
CAGCTGGGGG ACAGCTCTGG GGAGGGGCCA GAGTTTGTGG AGGAGGAGGA AGAGGTGGCT 
CTGCGCTCAG ACAGTGAGGG CAGCGACTAT ACTCCTGGCA AGAAGAAGAA GAAGAAGCTT 
GGACCTAAGA AAGAGAAGAA GAGCAAATCC AAGCGGAAGG AGGAGGAGGA GGAGGAGGAT 
GATGATGATG ATTCAAAGGA GCCTAAATCA TCTGCTCAGC TCCTGGAAGA CTGGGGCATG 
GAAGACATTG ACCACGTGTT CTCAGAGGAG GATTATCGAA CCCTCACCAA CTACAAGGCC 
TTCAGCCAGT TTGTCAGACC CCTCATTGCT GCCAAAAATC CCAAGATTGC TGTCTCCAAG 
ATGATGATGG TTTTGGGTGC AAAATGGCGG GAGTTCAGTA CCAATAACCC CTTCAAAGGC 
AGTTCTGGGG CATCAGTGGC AGCTGCGGCA GCAGCAGCGG TAGCTGTGGT GGAGAGCATG 
GTGACAGCCA CTGAGGTTGC ACCACCACCT CCCCCTGTGG AGGTGCCTAT CCGCAAGGCC 
AAGACCAAGG AGGGCAAAGG TCCCAATGCT CGGAGGAAGC CCAAGGGCAG CCCTCGTGTA 
CCTGATGCCA AGAAGCCTAA ACCCAAGAAA GTAGCTCCCC TGAAAATCAA GCTGGGAGGT 
TTTGGTTCCA AGCGTAAGAG ATCCTCGAGT GAGGATGATG ACTTAGATGT GGAATCTGAC 
TTCGATGATG CCAGTATCAA TAGCTATTCT GTTTCTGATG GTTCCACCAG CCGTAGTAGC 
CGCAGCCGCA AGAAACTCCG AACCACTAAA AAGAAAAAGA AAGGCGAGGA GGAGGTGACT 
GCTGTGGATG GTTATGAGAC AGACCACCAG GACTATTGCG AGGTGTGCCA GCAAGGCGGT 
GAGATCATCC TGTGTGATAC CTGTCCCCGT GCTTACCACA TGGTCTGCCT GGATCCCGAC 
ATGGAGAAGG CTCCCGAGGG CAAGTGGAGC TGCCCACACT GCGAGAAGGA AGGCATCCAG 
TGGGAAGCTA AAGAGGACAA TTCGGAGGGT GAGGAGATCC TGGAAGAGGT TGGGGGAGAC 
CTCGAAGAGG AGGATGACCA CCATATGGAA TTCTGTCGGG TCTGCAAGGA TGGTGGGGAA 
CTGCTCTGCT GTGATACCTG TCCTTCTTCC TACCACATCC ACTGCCTGAA TCCCCCACTT 
CCAGAGATCC CCAACGGTGA ATGGCTCTGT CCCCGTTGTA CGTGTCCAGC TCTGAAGGGC 
AAAGTGCAGA AGATCCTAAT CTGGAAGTGG GGTCAGCCAC CATCTCCCAC ACCAGTGCCT 
CGGCCTCCAG ATGCTGATCC CAACACGCCC TCCCCAAAGC CCTTGGAGGG GCGGCCAGAG 
CGGCAGTTCT TTGTGAAATG GCAAGGCATG TCTTACTGGC ACTGCTCCTG GGTTTCTGAA 
CTGCAGCTGG AGCTGCACTG TCAGGTGATG TTCCGAAACT ATCAGCGGAA GAATGATATG 
GATGAGCCAC CTTCTGGGGA CTTTGGTGGT GATGAAGAGA AAAGCCGAAA GCGAAAGAAC 
AAGGACCCTA AATTTGCAGA GATGGAGGAA CGCTTCTATC GCTATGGGAT AAAACCCGAG 
TGGATGATGA TCCACCGAAT CCTCAACCAC AGTGTGGACA AGAAGGGCCA CGTCCACTAC 
TTGATCAAGT GGCGGGACTT ACCTTACGAT CAGGCTTCTT GGGAGAGTGA GGATGTGGAG 
ATCCAGGATT ACGACCTGTT CAAGCAGAGC TATTGGAATC ACAGGGAGTT AATGAGGGGT 
GAGGAAGGCC GACCAGGCAA GAAGCTCAAG AAGGTGAAGC TTCGGAAGTT GGAGAGGCCT 
CCAGAAACGC CAACAGTTGA TCCAACAGTG AAGTATGAGC GACAGCCAGA GTACCTGGAT 
GCTACAGGTG GAACCCTGCA CCCCTATCAA ATGGAGGGCC TGAATTGGTT GCGCTTCTCC 
TGGGCTCAGG GCACTGACAC CATCTTGGCT GATGAGATGG GCCTTGGGAA AACTGTACAG 
ACAGCAGTCT TCCTGTATTC CCTTTACAAG GAGGGTCATT CCAAAGGCCC CTTCCTAGTG 
AGCGCCCCTC TTTCTACCAT CATCAACTGG GAGCGGGAGT TTGAAATGTG GGCTCCAGAC 
ATGTATGTCG TAACCTATGT GGGTGACAAG GACAGCCGTG CCATCATCCG AGAGAATGAG 
TTCTCCTTTG AAGACAATGC CATTCGTGGT GGCAAGAAGG CCTCCCGCAT GAAGAAAGAG 
GCATCTGTGA AATTCCATGT GCTGCTGACA TCCTATGAAT TGATCACCAT TGACATGGCT 
ATTTTGGGCT CTATTGATTG GGCCTGCCTC ATCGTGGATG AAGCCCATCG GCTGAAGAAC 
AATCAGTCTA AGTTCTTCCG GGTATTGAAT GGTTACTCAC TCCAGCACAA GCTGTTGCTG 
ACTGGGACAC CATTACAAAA CAATCTGGAA GAGTTGTTTC ATCTGCTCAA CTTTCTCACC 
CCCGAGAGGT TCCACAATTT GGAAGGTTTT TTGGAGGAGT TTGCTGACAT TGCCAAGGAG 
GACCAGATAA AAAAACTGCA TGACATGCTG GGGCCGCACA TGTTGCGGCG GCTCAAAGCC 
GATGTGTTCA AGAACATGCC CTCCAAGACA GAACTAATTG TGCGTGTGGA GCTGAGCCCT 
ATGCAGAAGA AATACTACAA GTACATCCTC ACTCGAAATT TTGAAGCACT CAATGCCCGA 
GGTGGTGGCA ACCAGGTGTC TCTGCTGAAT GTGGTGATGG ATCTTAAGAA GTGCTGCAAC 
CATCCATACC TCTTCCCTGT GGCTGCAATG GAAGCTCCTA AGATGCCTAA TGGCATGTAT 
GATGGCAGTG CCCTAATCAG AGCATCTGGG AAATTATTGC TGCTGCAGAA AATGCTCAAG 
AACCTTAAGG AGGGTGGGCA TCGTGTACTC ATCTTTTCCC AGATGACCAA GATGCTAGAC 
CTGCTAGAGG ATTTCTTGGA ACATGAAGGT TATAAATACG AACGCATCGA TGGTGGAATC 
ACTGGGAACA TGCGGCAAGA GGCCATTGAC CGCTTCAATG CACCGGGTGC TCAGCAGTTC 
TGCTTCTTGC TTTCCACTCG AGCTGGGGGC CTTGGAATCA ATCTGGCCAC TGCTGACACA 
GTTATTATCT ATGACTCTGA CTGGAACCCC CATAATGACA TTCAGGCCTT TAGCAGAGCT 
CACCGGATTG GGCAAAATAA AAAGGTAATG ATCTACCGGT TTGTGACCCG TGCGTCAGTG 
GAGGAGCGCA TCACGCAGGT GGCAAAGAAG AAAATGATGC TGACGCATCT AGTGGTGCGG 
CCTGGGCTGG GCTCCAAGAC TGGATCTATG TCCAAACAGG AGCTTGATGA TATCCTCAAA 
TTTGGCACTG AGGAACTATT CAAGGATGAA GCCACTGATG GAGGAGGAGA CAACAAAGAG 
GGAGAAGATA GCAGTGTTAT CCACTACGAT GATAAGGCCA TTGAACGGCT GCTAGACCGT 
AACCAGGATG AGACTGAAGA CACAGAATTG CAGGGCATGA ATGAATATTT GAGCTCATTC 
AAAGTGGCCC AGTATGTGGT ACGGGAAGAA GAAATGGGGG AGGAAGAGGA GGTAGAACGG 
GAAATCATTA AACAGGAAGA AAGTGTGGAT CCTGACTACT GGGAGAAATT GCTGCGGCAC 
CATTATGAGC AGCAGCAAGA AGATCTAGCC CGAAATCTGG GCAAAGGAAA AAGAATCCGT 
AAACAGGTCA ACTACAATGA TGGCTCCCAG GAGGACCGAG GTGTGTGTGG CCGGCCCCGC 
CCCCCACCCA TGGGCCGTTC CACTAGAGCA GTGGGCCCCG CTCATCTGCC CTCTCTCCCT 
CCAGATTGGC AGGACGACCA GTCCGACAAC CAGTCCGATT ACTCAGTGGC TTCAGAGGAA 
GGTGATGAAG ACTTTGATGA ACGTTCAGAA GCTCCCCGTA GGCCCAGTCG TAAGGGCCTG 
CGGAATGATA AAGATAAGCC ATTGCCTCCT CTGTTGGCCC GTGTTGGTGG GAATATTGAA 
GTACTTGGTT TTAATGCTCG TCAGCGAAAA GCCTTTCTTA ATGCAATTAT GCGATATGGT 
ATGCCACCTC AGGATGCTTT TACTACCCAG TGGCTTGTAA GAGACCTGCG AGGCAAATCA 
GAGAAAGAGT TCAAGGCATA TGTCTCTCTT TTCATGCGGC ATTTATGTGA GCCGGGGGCA 
GATGGGGCTG AGACCTTTGC TGATGGTGTC CCCCGAGAAG GCCTGTCTCG CCAGCATGTC 
CTTACTAGAA TTGGTGTTAT GTCTTTGATT CGCAAGAAGG TTCAGGAGTT TGAACATGTT 
AATGGGCGCT GGAGCATGCC TGAACTGGCT GAGGTGGAGG AAAACAAGAA GATGTCCCAG 
CCAGGGTCAC CCTCCCCAAA AACTCCTACA CCCTCCACTC CAGGGGACAC GCAGCCCAAC 
ACTCCTGCAC CTGTCCCACC TGCTGAAGAT GGGATAAAAA TAGAGGAAAA TAGCCTCAAA 
GAAGAAGAGA GCATAGAAGG AGAAAAGGAG GTTAAATCTA CAGCCCCTGA GACTGCCATT 
GAGTGTACAC AGGCCCCTGC CCCTGCCTCA GAGGATGAAA AGGTCGTTGT TGAACCCCCT 
GAGGGAGAGG AGAAAGTGGA AAAGGCAGAG GTGAAGGAGA GAACAGAGGA ACCTATGGAG 
ACAGAGCCCA AAGGTGCTGC TGATGTAGAG AAGGTGGAGG AAAAGTCAGC AATAGATCTG 
ACCCCTATTG TGGTAGAAGA CAAAGAAGAG AAGAAAGAAG AAGAAGAGAA AAAAGAGGTG 
ATGCTTCAGA ATGGAGAGAC CCCCAAGGAC CTGAATGATG AGAAACAGAA GAAAAATATT 
AAACAACGTT TCATGTTTAA CATTGCAGAT GGTGGTTTTA CTGAGTTGCA CTCCCTTTGG 
CAGAATGAAG AGCGGGCAGC CACAGTTACC AAGAAGACTT ATGAGATCTG GCATCGACGG 
CATGACTACT GGCTGCTAGC CGGCATTATA AACCATGGCT ATGCCCGGTG GCAAGACATC 
CAGAATGACC CACGCTATGC CATCCTCAAT GAGCCTTTCA AGGGTGAAAT GAACCGTGGC 
AATTTCTTAG AGATCAAGAA TAAATTTCTA GCTCGAAGGT TTAAGCTCTT AGAACAAGCT 
CTGGTGATTG AGGAACAGCT GCGCCGGGCT GCTTACTTGA ACATGTCAGA AGACCCTTCT 
CACCCTTCCA TGGCCCTCAA CACCCGCTTT GCTGAGGTGG AGTGTTTGGC GGAAAGTCAT 
CAGCACCTGT CCAAGGAGTC AATGGCAGGA AACAAGCCAG CCAATGCAGT CCTGCACAAA 
GGTATTCTGA AACAGCTGGA AGAACTGCTG AGTGACATGA AAGCTGATGT GACTCGACTC 
CCAGCTACCA TTGCCCGAAT TCCCCCAGTT GCTGTGAGGT TACAGATGTC AGAGCGTAAC 
ATTCTCAGCC GCCTGGCAAA CCGGGCACCC GAACCTACCC CACAGCAGGT AGCCCAGCAG 
CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHD4 ( XM_024448803.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024304571.1 Homo sapiens chromodomain helicase DNA binding protein 4 (CHD4), transcript variant X3, mRNA.

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CloneID	OHu103675
Clone ID Related Accession (Same CDS sequence)	XM_024448803.1
Accession Version	XM_024448803.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5805bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-25
Organism	Homo sapiens(human)
Product	chromodomain-helicase-DNA-binding protein 4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCGG GCCTGGGCTC CCCGTCCCCC TGCTCGGCGG GCAGTGAGGA GGAGGATATG 
GATGCACTTT TGAACAACAG CCTGCCCCCA CCCCACCCAG AAAATGAAGA GGACCCAGAA 
GAGGATTTGT CAGAAACAGA GACTCCAAAG CTCAAGAAGA AGAAAAAGCC TAAGAAACCT 
CGGGACCCTA AAATCCCTAA GAGCAAGCGC CAAAAAAAGG AGCTGGGGGA CAGCTCTGGG 
GAGGGGCCAG AGTTTGTGGA GGAGGAGGAA GAGGTGGCTC TGCGCTCAGA CAGTGAGGGC 
AGCGACTATA CTCCTGGCAA GAAGAAGAAG AAGAAGCTTG GACCTAAGAA AGAGAAGAAG 
AGCAAATCCA AGCGGAAGGA GGAGGAGGAG GAGGAGGATG ATGATGATGA TTCAAAGGAG 
CCTAAATCAT CTGCTCAGCT CCTGGAAGAC TGGGGCATGG AAGACATTGA CCACGTGTTC 
TCAGAGGAGG ATTATCGAAC CCTCACCAAC TACAAGGCCT TCAGCCAGTT TGTCAGACCC 
CTCATTGCTG CCAAAAATCC CAAGATTGCT GTCTCCAAGA TGATGATGGT TTTGGGTGCA 
AAATGGCGGG AGTTCAGTAC CAATAACCCC TTCAAAGGCA GTTCTGGGGC ATCAGTGGCA 
GCTGCGGCAG CAGCAGCGGT AGCTGTGGTG GAGAGCATGG TGACAGCCAC TGAGGTTGCA 
CCACCACCTC CCCCTGTGGA GGTGCCTATC CGCAAGGCCA AGACCAAGGA GGGCAAAGGT 
CCCAATGCTC GGAGGAAGCC CAAGGGCAGC CCTCGTGTAC CTGATGCCAA GAAGCCTAAA 
CCCAAGAAAG TAGCTCCCCT GAAAATCAAG CTGGGAGGTT TTGGTTCCAA GCGTAAGAGA 
TCCTCGAGTG AGGATGATGA CTTAGATGTG GAATCTGACT TCGATGATGC CAGTATCAAT 
AGCTATTCTG TTTCTGATGG TTCCACCAGC CGTAGTAGCC GCAGCCGCAA GAAACTCCGA 
ACCACTAAAA AGAAAAAGAA AGGCGAGGAG GAGGTGACTG CTGTGGATGG TTATGAGACA 
GACCACCAGG ACTATTGCGA GGTGTGCCAG CAAGGCGGTG AGATCATCCT GTGTGATACC 
TGTCCCCGTG CTTACCACAT GGTCTGCCTG GATCCCGACA TGGAGAAGGC TCCCGAGGGC 
AAGTGGAGCT GCCCACACTG CGAGAAGGAA GGCATCCAGT GGGAAGCTAA AGAGGACAAT 
TCGGAGGGTG AGGAGATCCT GGAAGAGGTT GGGGGAGACC TCGAAGAGGA GGATGACCAC 
CATATGGAAT TCTGTCGGGT CTGCAAGGAT GGTGGGGAAC TGCTCTGCTG TGATACCTGT 
CCTTCTTCCT ACCACATCCA CTGCCTGAAT CCCCCACTTC CAGAGATCCC CAACGGTGAA 
TGGCTCTGTC CCCGTTGTAC GTGTCCAGCT CTGAAGGGCA AAGTGCAGAA GATCCTAATC 
TGGAAGTGGG GTCAGCCACC ATCTCCCACA CCAGTGCCTC GGCCTCCAGA TGCTGATCCC 
AACACGCCCT CCCCAAAGCC CTTGGAGGGG CGGCCAGAGC GGCAGTTCTT TGTGAAATGG 
CAAGGCATGT CTTACTGGCA CTGCTCCTGG GTTTCTGAAC TGCAGCTGGA GCTGCACTGT 
CAGGTGATGT TCCGAAACTA TCAGCGGAAG AATGATATGG ATGAGCCACC TTCTGGGGAC 
TTTGGTGGTG ATGAAGAGAA AAGCCGAAAG CGAAAGAACA AGGACCCTAA ATTTGCAGAG 
ATGGAGGAAC GCTTCTATCG CTATGGGATA AAACCCGAGT GGATGATGAT CCACCGAATC 
CTCAACCACA GTGTGGACAA GAAGGGCCAC GTCCACTACT TGATCAAGTG GCGGGACTTA 
CCTTACGATC AGGCTTCTTG GGAGAGTGAG GATGTGGAGA TCCAGGATTA CGACCTGTTC 
AAGCAGAGCT ATTGGAATCA CAGGGAGTTA ATGAGGGGTG AGGAAGGCCG ACCAGGCAAG 
AAGCTCAAGA AGGTGAAGCT TCGGAAGTTG GAGAGGCCTC CAGAAACGCC AACAGTTGAT 
CCAACAGTGA AGTATGAGCG ACAGCCAGAG TACCTGGATG CTACAGGTGG AACCCTGCAC 
CCCTATCAAA TGGAGGGCCT GAATTGGTTG CGCTTCTCCT GGGCTCAGGG CACTGACACC 
ATCTTGGCTG ATGAGATGGG CCTTGGGAAA ACTGTACAGA CAGCAGTCTT CCTGTATTCC 
CTTTACAAGG AGGGTCATTC CAAAGGCCCC TTCCTAGTGA GCGCCCCTCT TTCTACCATC 
ATCAACTGGG AGCGGGAGTT TGAAATGTGG GCTCCAGACA TGTATGTCGT AACCTATGTG 
GGTGACAAGG ACAGCCGTGC CATCATCCGA GAGAATGAGT TCTCCTTTGA AGACAATGCC 
ATTCGTGGTG GCAAGAAGGC CTCCCGCATG AAGAAAGAGG CATCTGTGAA ATTCCATGTG 
CTGCTGACAT CCTATGAATT GATCACCATT GACATGGCTA TTTTGGGCTC TATTGATTGG 
GCCTGCCTCA TCGTGGATGA AGCCCATCGG CTGAAGAACA ATCAGTCTAA GTTCTTCCGG 
GTATTGAATG GTTACTCACT CCAGCACAAG CTGTTGCTGA CTGGGACACC ATTACAAAAC 
AATCTGGAAG AGTTGTTTCA TCTGCTCAAC TTTCTCACCC CCGAGAGGTT CCACAATTTG 
GAAGGTTTTT TGGAGGAGTT TGCTGACATT GCCAAGGAGG ACCAGATAAA AAAACTGCAT 
GACATGCTGG GGCCGCACAT GTTGCGGCGG CTCAAAGCCG ATGTGTTCAA GAACATGCCC 
TCCAAGACAG AACTAATTGT GCGTGTGGAG CTGAGCCCTA TGCAGAAGAA ATACTACAAG 
TACATCCTCA CTCGAAATTT TGAAGCACTC AATGCCCGAG GTGGTGGCAA CCAGGTGTCT 
CTGCTGAATG TGGTGATGGA TCTTAAGAAG TGCTGCAACC ATCCATACCT CTTCCCTGTG 
GCTGCAATGG AAGCTCCTAA GATGCCTAAT GGCATGTATG ATGGCAGTGC CCTAATCAGA 
GCATCTGGGA AATTATTGCT GCTGCAGAAA ATGCTCAAGA ACCTTAAGGA GGGTGGGCAT 
CGTGTACTCA TCTTTTCCCA GATGACCAAG ATGCTAGACC TGCTAGAGGA TTTCTTGGAA 
CATGAAGGTT ATAAATACGA ACGCATCGAT GGTGGAATCA CTGGGAACAT GCGGCAAGAG 
GCCATTGACC GCTTCAATGC ACCGGGTGCT CAGCAGTTCT GCTTCTTGCT TTCCACTCGA 
GCTGGGGGCC TTGGAATCAA TCTGGCCACT GCTGACACAG TTATTATCTA TGACTCTGAC 
TGGAACCCCC ATAATGACAT TCAGGCCTTT AGCAGAGCTC ACCGGATTGG GCAAAATAAA 
AAGGTAATGA TCTACCGGTT TGTGACCCGT GCGTCAGTGG AGGAGCGCAT CACGCAGGTG 
GCAAAGAAGA AAATGATGCT GACGCATCTA GTGGTGCGGC CTGGGCTGGG CTCCAAGACT 
GGATCTATGT CCAAACAGGA GCTTGATGAT ATCCTCAAAT TTGGCACTGA GGAACTATTC 
AAGGATGAAG CCACTGATGG AGGAGGAGAC AACAAAGAGG GAGAAGATAG CAGTGTTATC 
CACTACGATG ATAAGGCCAT TGAACGGCTG CTAGACCGTA ACCAGGATGA GACTGAAGAC 
ACAGAATTGC AGGGCATGAA TGAATATTTG AGCTCATTCA AAGTGGCCCA GTATGTGGTA 
CGGGAAGAAG AAATGGGGGA GGAAGAGGAG GTAGAACGGG AAATCATTAA ACAGGAAGAA 
AGTGTGGATC CTGACTACTG GGAGAAATTG CTGCGGCACC ATTATGAGCA GCAGCAAGAA 
GATCTAGCCC GAAATCTGGG CAAAGGAAAA AGAATCCGTA AACAGGTCAA CTACAATGAT 
GGCTCCCAGG AGGACCGAGG TGTGTGTGGC CGGCCCCGCC CCCCACCCAT GGGCCGTTCC 
ACTAGAGCAG TGGGCCCCGC TCATCTGCCC TCTCTCCCTC CAGATTGGCA GGACGACCAG 
TCCGACAACC AGTCCGATTA CTCAGTGGCT TCAGAGGAAG GTGATGAAGA CTTTGATGAA 
CGTTCAGAAG CTCCCCGTAG GCCCAGTCGT AAGGGCCTGC GGAATGATAA AGATAAGCCA 
TTGCCTCCTC TGTTGGCCCG TGTTGGTGGG AATATTGAAG TACTTGGTTT TAATGCTCGT 
CAGCGAAAAG CCTTTCTTAA TGCAATTATG CGATATGGTA TGCCACCTCA GGATGCTTTT 
ACTACCCAGT GGCTTGTAAG AGACCTGCGA GGCAAATCAG AGAAAGAGTT CAAGGCATAT 
GTCTCTCTTT TCATGCGGCA TTTATGTGAG CCGGGGGCAG ATGGGGCTGA GACCTTTGCT 
GATGGTGTCC CCCGAGAAGG CCTGTCTCGC CAGCATGTCC TTACTAGAAT TGGTGTTATG 
TCTTTGATTC GCAAGAAGGT TCAGGAGTTT GAACATGTTA ATGGGCGCTG GAGCATGCCT 
GAACTGGCTG AGGTGGAGGA AAACAAGAAG ATGTCCCAGC CAGGGTCACC CTCCCCAAAA 
ACTCCTACAC CCTCCACTCC AGGGGACACG CAGCCCAACA CTCCTGCACC TGTCCCACCT 
GCTGAAGATG GGATAAAAAT AGAGGAAAAT AGCCTCAAAG AAGAAGAGAG CATAGAAGGA 
GAAAAGGAGG TTAAATCTAC AGCCCCTGAG ACTGCCATTG AGTGTACACA GGCCCCTGCC 
CCTGCCTCAG AGGATGAAAA GGTCGTTGTT GAACCCCCTG AGGGAGAGGA GAAAGTGGAA 
AAGGCAGAGG TGAAGGAGAG AACAGAGGAA CCTATGGAGA CAGAGCCCAA AGGTGCTGCT 
GATGTAGAGA AGGTGGAGGA AAAGTCAGCA ATAGATCTGA CCCCTATTGT GGTAGAAGAC 
AAAGAAGAGA AGAAAGAAGA AGAAGAGAAA AAAGAGGTGA TGCTTCAGAA TGGAGAGACC 
CCCAAGGACC TGAATGATGA GAAACAGAAG AAAAATATTA AACAACGTTT CATGTTTAAC 
ATTGCAGATG GTGGTTTTAC TGAGTTGCAC TCCCTTTGGC AGAATGAAGA GCGGGCAGCC 
ACAGTTACCA AGAAGACTTA TGAGATCTGG CATCGACGGC ATGACTACTG GCTGCTAGCC 
GGCATTATAA ACCATGGCTA TGCCCGGTGG CAAGACATCC AGAATGACCC ACGCTATGCC 
ATCCTCAATG AGCCTTTCAA GGGTGAAATG AACCGTGGCA ATTTCTTAGA GATCAAGAAT 
AAATTTCTAG CTCGAAGGTT TAAGCTCTTA GAACAAGCTC TGGTGATTGA GGAACAGCTG 
CGCCGGGCTG CTTACTTGAA CATGTCAGAA GACCCTTCTC ACCCTTCCAT GGCCCTCAAC 
ACCCGCTTTG CTGAGGTGGA GTGTTTGGCG GAAAGTCATC AGCACCTGTC CAAGGAGTCA 
ATGGCAGGAA ACAAGCCAGC CAATGCAGTC CTGCACAAAG GTATTCTGAA ACAGCTGGAA 
GAACTGCTGA GTGACATGAA AGCTGATGTG ACTCGACTCC CAGCTACCAT TGCCCGAATT 
CCCCCAGTTG CTGTGAGGTT ACAGATGTCA GAGCGTAACA TTCTCAGCCG CCTGGCAAAC 
CGGGCACCCG AACCTACCCC ACAGCAGGTA GCCCAGCAGC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024304571.1
CDS	165..5969
Translation	MASGLGSPSPCSAGSEEEDMDALLNNSLPPPHPENEEDPEEDLSETETPKLKKKKKPKKPRDPKIPKSKRQKKELGDSSGEGPEFVEEEEEVALRSDSEGSDYTPGKKKKKKLGPKKEKKSKSKRKEEEEEEDDDDDSKEPKSSAQLLEDWGMEDIDHVFSEEDYRTLTNYKAFSQFVRPLIAAKNPKIAVSKMMMVLGAKWREFSTNNPFKGSSGASVAAAAAAAVAVVESMVTATEVAPPPPPVEVPIRKAKTKEGKGPNARRKPKGSPRVPDAKKPKPKKVAPLKIKLGGFGSKRKRSSSEDDDLDVESDFDDASINSYSVSDGSTSRSSRSRKKLRTTKKKKKGEEEVTAVDGYETDHQDYCEVCQQGGEIILCDTCPRAYHMVCLDPDMEKAPEGKWSCPHCEKEGIQWEAKEDNSEGEEILEEVGGDLEEEDDHHMEFCRVCKDGGELLCCDTCPSSYHIHCLNPPLPEIPNGEWLCPRCTCPALKGKVQKILIWKWGQPPSPTPVPRPPDADPNTPSPKPLEGRPERQFFVKWQGMSYWHCSWVSELQLELHCQVMFRNYQRKNDMDEPPSGDFGGDEEKSRKRKNKDPKFAEMEERFYRYGIKPEWMMIHRILNHSVDKKGHVHYLIKWRDLPYDQASWESEDVEIQDYDLFKQSYWNHRELMRGEEGRPGKKLKKVKLRKLERPPETPTVDPTVKYERQPEYLDATGGTLHPYQMEGLNWLRFSWAQGTDTILADEMGLGKTVQTAVFLYSLYKEGHSKGPFLVSAPLSTIINWEREFEMWAPDMYVVTYVGDKDSRAIIRENEFSFEDNAIRGGKKASRMKKEASVKFHVLLTSYELITIDMAILGSIDWACLIVDEAHRLKNNQSKFFRVLNGYSLQHKLLLTGTPLQNNLEELFHLLNFLTPERFHNLEGFLEEFADIAKEDQIKKLHDMLGPHMLRRLKADVFKNMPSKTELIVRVELSPMQKKYYKYILTRNFEALNARGGGNQVSLLNVVMDLKKCCNHPYLFPVAAMEAPKMPNGMYDGSALIRASGKLLLLQKMLKNLKEGGHRVLIFSQMTKMLDLLEDFLEHEGYKYERIDGGITGNMRQEAIDRFNAPGAQQFCFLLSTRAGGLGINLATADTVIIYDSDWNPHNDIQAFSRAHRIGQNKKVMIYRFVTRASVEERITQVAKKKMMLTHLVVRPGLGSKTGSMSKQELDDILKFGTEELFKDEATDGGGDNKEGEDSSVIHYDDKAIERLLDRNQDETEDTELQGMNEYLSSFKVAQYVVREEEMGEEEEVEREIIKQEESVDPDYWEKLLRHHYEQQQEDLARNLGKGKRIRKQVNYNDGSQEDRGVCGRPRPPPMGRSTRAVGPAHLPSLPPDWQDDQSDNQSDYSVASEEGDEDFDERSEAPRRPSRKGLRNDKDKPLPPLLARVGGNIEVLGFNARQRKAFLNAIMRYGMPPQDAFTTQWLVRDLRGKSEKEFKAYVSLFMRHLCEPGADGAETFADGVPREGLSRQHVLTRIGVMSLIRKKVQEFEHVNGRWSMPELAEVEENKKMSQPGSPSPKTPTPSTPGDTQPNTPAPVPPAEDGIKIEENSLKEEESIEGEKEVKSTAPETAIECTQAPAPASEDEKVVVEPPEGEEKVEKAEVKERTEEPMETEPKGAADVEKVEEKSAIDLTPIVVEDKEEKKEEEEKKEVMLQNGETPKDLNDEKQKKNIKQRFMFNIADGGFTELHSLWQNEERAATVTKKTYEIWHRRHDYWLLAGIINHGYARWQDIQNDPRYAILNEPFKGEMNRGNFLEIKNKFLARRFKLLEQALVIEEQLRRAAYLNMSEDPSHPSMALNTRFAEVECLAESHQHLSKESMAGNKPANAVLHKGILKQLEELLSDMKADVTRLPATIARIPPVAVRLQMSERNILSRLANRAPEPTPQQVAQQQ

Target ORF information:

RefSeq Version	XM_024448803.1
Organism	Homo sapiens(human)
Definition	Homo sapiens chromodomain helicase DNA binding protein 4 (CHD4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024448803.1

ORF Insert Sequence:

ATGGCGTCGG GCCTGGGCTC CCCGTCCCCC TGCTCGGCGG GCAGTGAGGA GGAGGATATG 
GATGCACTTT TGAACAACAG CCTGCCCCCA CCCCACCCAG AAAATGAAGA GGACCCAGAA 
GAGGATTTGT CAGAAACAGA GACTCCAAAG CTCAAGAAGA AGAAAAAGCC TAAGAAACCT 
CGGGACCCTA AAATCCCTAA GAGCAAGCGC CAAAAAAAGG AGCTGGGGGA CAGCTCTGGG 
GAGGGGCCAG AGTTTGTGGA GGAGGAGGAA GAGGTGGCTC TGCGCTCAGA CAGTGAGGGC 
AGCGACTATA CTCCTGGCAA GAAGAAGAAG AAGAAGCTTG GACCTAAGAA AGAGAAGAAG 
AGCAAATCCA AGCGGAAGGA GGAGGAGGAG GAGGAGGATG ATGATGATGA TTCAAAGGAG 
CCTAAATCAT CTGCTCAGCT CCTGGAAGAC TGGGGCATGG AAGACATTGA CCACGTGTTC 
TCAGAGGAGG ATTATCGAAC CCTCACCAAC TACAAGGCCT TCAGCCAGTT TGTCAGACCC 
CTCATTGCTG CCAAAAATCC CAAGATTGCT GTCTCCAAGA TGATGATGGT TTTGGGTGCA 
AAATGGCGGG AGTTCAGTAC CAATAACCCC TTCAAAGGCA GTTCTGGGGC ATCAGTGGCA 
GCTGCGGCAG CAGCAGCGGT AGCTGTGGTG GAGAGCATGG TGACAGCCAC TGAGGTTGCA 
CCACCACCTC CCCCTGTGGA GGTGCCTATC CGCAAGGCCA AGACCAAGGA GGGCAAAGGT 
CCCAATGCTC GGAGGAAGCC CAAGGGCAGC CCTCGTGTAC CTGATGCCAA GAAGCCTAAA 
CCCAAGAAAG TAGCTCCCCT GAAAATCAAG CTGGGAGGTT TTGGTTCCAA GCGTAAGAGA 
TCCTCGAGTG AGGATGATGA CTTAGATGTG GAATCTGACT TCGATGATGC CAGTATCAAT 
AGCTATTCTG TTTCTGATGG TTCCACCAGC CGTAGTAGCC GCAGCCGCAA GAAACTCCGA 
ACCACTAAAA AGAAAAAGAA AGGCGAGGAG GAGGTGACTG CTGTGGATGG TTATGAGACA 
GACCACCAGG ACTATTGCGA GGTGTGCCAG CAAGGCGGTG AGATCATCCT GTGTGATACC 
TGTCCCCGTG CTTACCACAT GGTCTGCCTG GATCCCGACA TGGAGAAGGC TCCCGAGGGC 
AAGTGGAGCT GCCCACACTG CGAGAAGGAA GGCATCCAGT GGGAAGCTAA AGAGGACAAT 
TCGGAGGGTG AGGAGATCCT GGAAGAGGTT GGGGGAGACC TCGAAGAGGA GGATGACCAC 
CATATGGAAT TCTGTCGGGT CTGCAAGGAT GGTGGGGAAC TGCTCTGCTG TGATACCTGT 
CCTTCTTCCT ACCACATCCA CTGCCTGAAT CCCCCACTTC CAGAGATCCC CAACGGTGAA 
TGGCTCTGTC CCCGTTGTAC GTGTCCAGCT CTGAAGGGCA AAGTGCAGAA GATCCTAATC 
TGGAAGTGGG GTCAGCCACC ATCTCCCACA CCAGTGCCTC GGCCTCCAGA TGCTGATCCC 
AACACGCCCT CCCCAAAGCC CTTGGAGGGG CGGCCAGAGC GGCAGTTCTT TGTGAAATGG 
CAAGGCATGT CTTACTGGCA CTGCTCCTGG GTTTCTGAAC TGCAGCTGGA GCTGCACTGT 
CAGGTGATGT TCCGAAACTA TCAGCGGAAG AATGATATGG ATGAGCCACC TTCTGGGGAC 
TTTGGTGGTG ATGAAGAGAA AAGCCGAAAG CGAAAGAACA AGGACCCTAA ATTTGCAGAG 
ATGGAGGAAC GCTTCTATCG CTATGGGATA AAACCCGAGT GGATGATGAT CCACCGAATC 
CTCAACCACA GTGTGGACAA GAAGGGCCAC GTCCACTACT TGATCAAGTG GCGGGACTTA 
CCTTACGATC AGGCTTCTTG GGAGAGTGAG GATGTGGAGA TCCAGGATTA CGACCTGTTC 
AAGCAGAGCT ATTGGAATCA CAGGGAGTTA ATGAGGGGTG AGGAAGGCCG ACCAGGCAAG 
AAGCTCAAGA AGGTGAAGCT TCGGAAGTTG GAGAGGCCTC CAGAAACGCC AACAGTTGAT 
CCAACAGTGA AGTATGAGCG ACAGCCAGAG TACCTGGATG CTACAGGTGG AACCCTGCAC 
CCCTATCAAA TGGAGGGCCT GAATTGGTTG CGCTTCTCCT GGGCTCAGGG CACTGACACC 
ATCTTGGCTG ATGAGATGGG CCTTGGGAAA ACTGTACAGA CAGCAGTCTT CCTGTATTCC 
CTTTACAAGG AGGGTCATTC CAAAGGCCCC TTCCTAGTGA GCGCCCCTCT TTCTACCATC 
ATCAACTGGG AGCGGGAGTT TGAAATGTGG GCTCCAGACA TGTATGTCGT AACCTATGTG 
GGTGACAAGG ACAGCCGTGC CATCATCCGA GAGAATGAGT TCTCCTTTGA AGACAATGCC 
ATTCGTGGTG GCAAGAAGGC CTCCCGCATG AAGAAAGAGG CATCTGTGAA ATTCCATGTG 
CTGCTGACAT CCTATGAATT GATCACCATT GACATGGCTA TTTTGGGCTC TATTGATTGG 
GCCTGCCTCA TCGTGGATGA AGCCCATCGG CTGAAGAACA ATCAGTCTAA GTTCTTCCGG 
GTATTGAATG GTTACTCACT CCAGCACAAG CTGTTGCTGA CTGGGACACC ATTACAAAAC 
AATCTGGAAG AGTTGTTTCA TCTGCTCAAC TTTCTCACCC CCGAGAGGTT CCACAATTTG 
GAAGGTTTTT TGGAGGAGTT TGCTGACATT GCCAAGGAGG ACCAGATAAA AAAACTGCAT 
GACATGCTGG GGCCGCACAT GTTGCGGCGG CTCAAAGCCG ATGTGTTCAA GAACATGCCC 
TCCAAGACAG AACTAATTGT GCGTGTGGAG CTGAGCCCTA TGCAGAAGAA ATACTACAAG 
TACATCCTCA CTCGAAATTT TGAAGCACTC AATGCCCGAG GTGGTGGCAA CCAGGTGTCT 
CTGCTGAATG TGGTGATGGA TCTTAAGAAG TGCTGCAACC ATCCATACCT CTTCCCTGTG 
GCTGCAATGG AAGCTCCTAA GATGCCTAAT GGCATGTATG ATGGCAGTGC CCTAATCAGA 
GCATCTGGGA AATTATTGCT GCTGCAGAAA ATGCTCAAGA ACCTTAAGGA GGGTGGGCAT 
CGTGTACTCA TCTTTTCCCA GATGACCAAG ATGCTAGACC TGCTAGAGGA TTTCTTGGAA 
CATGAAGGTT ATAAATACGA ACGCATCGAT GGTGGAATCA CTGGGAACAT GCGGCAAGAG 
GCCATTGACC GCTTCAATGC ACCGGGTGCT CAGCAGTTCT GCTTCTTGCT TTCCACTCGA 
GCTGGGGGCC TTGGAATCAA TCTGGCCACT GCTGACACAG TTATTATCTA TGACTCTGAC 
TGGAACCCCC ATAATGACAT TCAGGCCTTT AGCAGAGCTC ACCGGATTGG GCAAAATAAA 
AAGGTAATGA TCTACCGGTT TGTGACCCGT GCGTCAGTGG AGGAGCGCAT CACGCAGGTG 
GCAAAGAAGA AAATGATGCT GACGCATCTA GTGGTGCGGC CTGGGCTGGG CTCCAAGACT 
GGATCTATGT CCAAACAGGA GCTTGATGAT ATCCTCAAAT TTGGCACTGA GGAACTATTC 
AAGGATGAAG CCACTGATGG AGGAGGAGAC AACAAAGAGG GAGAAGATAG CAGTGTTATC 
CACTACGATG ATAAGGCCAT TGAACGGCTG CTAGACCGTA ACCAGGATGA GACTGAAGAC 
ACAGAATTGC AGGGCATGAA TGAATATTTG AGCTCATTCA AAGTGGCCCA GTATGTGGTA 
CGGGAAGAAG AAATGGGGGA GGAAGAGGAG GTAGAACGGG AAATCATTAA ACAGGAAGAA 
AGTGTGGATC CTGACTACTG GGAGAAATTG CTGCGGCACC ATTATGAGCA GCAGCAAGAA 
GATCTAGCCC GAAATCTGGG CAAAGGAAAA AGAATCCGTA AACAGGTCAA CTACAATGAT 
GGCTCCCAGG AGGACCGAGG TGTGTGTGGC CGGCCCCGCC CCCCACCCAT GGGCCGTTCC 
ACTAGAGCAG TGGGCCCCGC TCATCTGCCC TCTCTCCCTC CAGATTGGCA GGACGACCAG 
TCCGACAACC AGTCCGATTA CTCAGTGGCT TCAGAGGAAG GTGATGAAGA CTTTGATGAA 
CGTTCAGAAG CTCCCCGTAG GCCCAGTCGT AAGGGCCTGC GGAATGATAA AGATAAGCCA 
TTGCCTCCTC TGTTGGCCCG TGTTGGTGGG AATATTGAAG TACTTGGTTT TAATGCTCGT 
CAGCGAAAAG CCTTTCTTAA TGCAATTATG CGATATGGTA TGCCACCTCA GGATGCTTTT 
ACTACCCAGT GGCTTGTAAG AGACCTGCGA GGCAAATCAG AGAAAGAGTT CAAGGCATAT 
GTCTCTCTTT TCATGCGGCA TTTATGTGAG CCGGGGGCAG ATGGGGCTGA GACCTTTGCT 
GATGGTGTCC CCCGAGAAGG CCTGTCTCGC CAGCATGTCC TTACTAGAAT TGGTGTTATG 
TCTTTGATTC GCAAGAAGGT TCAGGAGTTT GAACATGTTA ATGGGCGCTG GAGCATGCCT 
GAACTGGCTG AGGTGGAGGA AAACAAGAAG ATGTCCCAGC CAGGGTCACC CTCCCCAAAA 
ACTCCTACAC CCTCCACTCC AGGGGACACG CAGCCCAACA CTCCTGCACC TGTCCCACCT 
GCTGAAGATG GGATAAAAAT AGAGGAAAAT AGCCTCAAAG AAGAAGAGAG CATAGAAGGA 
GAAAAGGAGG TTAAATCTAC AGCCCCTGAG ACTGCCATTG AGTGTACACA GGCCCCTGCC 
CCTGCCTCAG AGGATGAAAA GGTCGTTGTT GAACCCCCTG AGGGAGAGGA GAAAGTGGAA 
AAGGCAGAGG TGAAGGAGAG AACAGAGGAA CCTATGGAGA CAGAGCCCAA AGGTGCTGCT 
GATGTAGAGA AGGTGGAGGA AAAGTCAGCA ATAGATCTGA CCCCTATTGT GGTAGAAGAC 
AAAGAAGAGA AGAAAGAAGA AGAAGAGAAA AAAGAGGTGA TGCTTCAGAA TGGAGAGACC 
CCCAAGGACC TGAATGATGA GAAACAGAAG AAAAATATTA AACAACGTTT CATGTTTAAC 
ATTGCAGATG GTGGTTTTAC TGAGTTGCAC TCCCTTTGGC AGAATGAAGA GCGGGCAGCC 
ACAGTTACCA AGAAGACTTA TGAGATCTGG CATCGACGGC ATGACTACTG GCTGCTAGCC 
GGCATTATAA ACCATGGCTA TGCCCGGTGG CAAGACATCC AGAATGACCC ACGCTATGCC 
ATCCTCAATG AGCCTTTCAA GGGTGAAATG AACCGTGGCA ATTTCTTAGA GATCAAGAAT 
AAATTTCTAG CTCGAAGGTT TAAGCTCTTA GAACAAGCTC TGGTGATTGA GGAACAGCTG 
CGCCGGGCTG CTTACTTGAA CATGTCAGAA GACCCTTCTC ACCCTTCCAT GGCCCTCAAC 
ACCCGCTTTG CTGAGGTGGA GTGTTTGGCG GAAAGTCATC AGCACCTGTC CAAGGAGTCA 
ATGGCAGGAA ACAAGCCAGC CAATGCAGTC CTGCACAAAG GTATTCTGAA ACAGCTGGAA 
GAACTGCTGA GTGACATGAA AGCTGATGTG ACTCGACTCC CAGCTACCAT TGCCCGAATT 
CCCCCAGTTG CTGTGAGGTT ACAGATGTCA GAGCGTAACA TTCTCAGCCG CCTGGCAAAC 
CGGGCACCCG AACCTACCCC ACAGCAGGTA GCCCAGCAGC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHD4 ( XM_024448804.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024304572.1 Homo sapiens chromodomain helicase DNA binding protein 4 (CHD4), transcript variant X4, mRNA.

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CloneID	OHu103676
Clone ID Related Accession (Same CDS sequence)	XM_024448804.1
Accession Version	XM_024448804.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5787bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-25
Organism	Homo sapiens(human)
Product	chromodomain-helicase-DNA-binding protein 4 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCGG GCCTGGGCTC CCCGTCCCCC TGCTCGGCGG GCAGTGAGGA GGAGGATATG 
GATGCACTTT TGAACAACAG CCTGCCCCCA CCCCACCCAG AAAATGAAGA GGACCCAGAA 
GAGGATTTGT CAGAAACAGA GACTCCAAAG CTCAAGAAGA AGAAAAAGCC TAAGAAACCT 
CGGGACCCTA AAATCCCTAA GAGCAAGCGC CAAAAAAAGG AGCGTATGCT CTTATGCCGG 
CAGCTGGGGG ACAGCTCTGG GGAGGGGCCA GAGTTTGTGG AGGAGGAGGA AGAGGTGGCT 
CTGCGCTCAG ACAGTGAGGG CAGCGACTAT ACTCCTGGCA AGAAGAAGAA GAAGAAGCTT 
GGACCTAAGA AAGAGAAGAA GAGCAAATCC AAGCGGAAGG AGGAGGAGGA GGAGGAGGAT 
GATGATGATG ATTCAAAGGA GCCTAAATCA TCTGCTCAGC TCCTGGAAGA CTGGGGCATG 
GAAGACATTG ACCACGTGTT CTCAGAGGAG GATTATCGAA CCCTCACCAA CTACAAGGCC 
TTCAGCCAGT TTGTCAGACC CCTCATTGCT GCCAAAAATC CCAAGATTGC TGTCTCCAAG 
ATGATGATGG TTTTGGGTGC AAAATGGCGG GAGTTCAGTA CCAATAACCC CTTCAAAGGC 
AGTTCTGGGG CATCAGTGGC AGCTGCGGCA GCAGCAGCGG TAGCTGTGGT GGAGAGCATG 
GTGACAGCCA CTGAGGTTGC ACCACCACCT CCCCCTGTGG AGGTGCCTAT CCGCAAGGCC 
AAGACCAAGG AGGGCAAAGG TCCCAATGCT CGGAGGAAGC CCAAGGGCAG CCCTCGTGTA 
CCTGATGCCA AGAAGCCTAA ACCCAAGAAA GTAGCTCCCC TGAAAATCAA GCTGGGAGGT 
TTTGGTTCCA AGCGTAAGAG ATCCTCGAGT GAGGATGATG ACTTAGATGT GGAATCTGAC 
TTCGATGATG CCAGTATCAA TAGCTATTCT GTTTCTGATG GTTCCACCAG CCGTAGTAGC 
CGCAGCCGCA AGAAACTCCG AACCACTAAA AAGAAAAAGA AAGACCACCA GGACTATTGC 
GAGGTGTGCC AGCAAGGCGG TGAGATCATC CTGTGTGATA CCTGTCCCCG TGCTTACCAC 
ATGGTCTGCC TGGATCCCGA CATGGAGAAG GCTCCCGAGG GCAAGTGGAG CTGCCCACAC 
TGCGAGAAGG AAGGCATCCA GTGGGAAGCT AAAGAGGACA ATTCGGAGGG TGAGGAGATC 
CTGGAAGAGG TTGGGGGAGA CCTCGAAGAG GAGGATGACC ACCATATGGA ATTCTGTCGG 
GTCTGCAAGG ATGGTGGGGA ACTGCTCTGC TGTGATACCT GTCCTTCTTC CTACCACATC 
CACTGCCTGA ATCCCCCACT TCCAGAGATC CCCAACGGTG AATGGCTCTG TCCCCGTTGT 
ACGTGTCCAG CTCTGAAGGG CAAAGTGCAG AAGATCCTAA TCTGGAAGTG GGGTCAGCCA 
CCATCTCCCA CACCAGTGCC TCGGCCTCCA GATGCTGATC CCAACACGCC CTCCCCAAAG 
CCCTTGGAGG GGCGGCCAGA GCGGCAGTTC TTTGTGAAAT GGCAAGGCAT GTCTTACTGG 
CACTGCTCCT GGGTTTCTGA ACTGCAGCTG GAGCTGCACT GTCAGGTGAT GTTCCGAAAC 
TATCAGCGGA AGAATGATAT GGATGAGCCA CCTTCTGGGG ACTTTGGTGG TGATGAAGAG 
AAAAGCCGAA AGCGAAAGAA CAAGGACCCT AAATTTGCAG AGATGGAGGA ACGCTTCTAT 
CGCTATGGGA TAAAACCCGA GTGGATGATG ATCCACCGAA TCCTCAACCA CAGTGTGGAC 
AAGAAGGGCC ACGTCCACTA CTTGATCAAG TGGCGGGACT TACCTTACGA TCAGGCTTCT 
TGGGAGAGTG AGGATGTGGA GATCCAGGAT TACGACCTGT TCAAGCAGAG CTATTGGAAT 
CACAGGGAGT TAATGAGGGG TGAGGAAGGC CGACCAGGCA AGAAGCTCAA GAAGGTGAAG 
CTTCGGAAGT TGGAGAGGCC TCCAGAAACG CCAACAGTTG ATCCAACAGT GAAGTATGAG 
CGACAGCCAG AGTACCTGGA TGCTACAGGT GGAACCCTGC ACCCCTATCA AATGGAGGGC 
CTGAATTGGT TGCGCTTCTC CTGGGCTCAG GGCACTGACA CCATCTTGGC TGATGAGATG 
GGCCTTGGGA AAACTGTACA GACAGCAGTC TTCCTGTATT CCCTTTACAA GGAGGGTCAT 
TCCAAAGGCC CCTTCCTAGT GAGCGCCCCT CTTTCTACCA TCATCAACTG GGAGCGGGAG 
TTTGAAATGT GGGCTCCAGA CATGTATGTC GTAACCTATG TGGGTGACAA GGACAGCCGT 
GCCATCATCC GAGAGAATGA GTTCTCCTTT GAAGACAATG CCATTCGTGG TGGCAAGAAG 
GCCTCCCGCA TGAAGAAAGA GGCATCTGTG AAATTCCATG TGCTGCTGAC ATCCTATGAA 
TTGATCACCA TTGACATGGC TATTTTGGGC TCTATTGATT GGGCCTGCCT CATCGTGGAT 
GAAGCCCATC GGCTGAAGAA CAATCAGTCT AAGTTCTTCC GGGTATTGAA TGGTTACTCA 
CTCCAGCACA AGCTGTTGCT GACTGGGACA CCATTACAAA ACAATCTGGA AGAGTTGTTT 
CATCTGCTCA ACTTTCTCAC CCCCGAGAGG TTCCACAATT TGGAAGGTTT TTTGGAGGAG 
TTTGCTGACA TTGCCAAGGA GGACCAGATA AAAAAACTGC ATGACATGCT GGGGCCGCAC 
ATGTTGCGGC GGCTCAAAGC CGATGTGTTC AAGAACATGC CCTCCAAGAC AGAACTAATT 
GTGCGTGTGG AGCTGAGCCC TATGCAGAAG AAATACTACA AGTACATCCT CACTCGAAAT 
TTTGAAGCAC TCAATGCCCG AGGTGGTGGC AACCAGGTGT CTCTGCTGAA TGTGGTGATG 
GATCTTAAGA AGTGCTGCAA CCATCCATAC CTCTTCCCTG TGGCTGCAAT GGAAGCTCCT 
AAGATGCCTA ATGGCATGTA TGATGGCAGT GCCCTAATCA GAGCATCTGG GAAATTATTG 
CTGCTGCAGA AAATGCTCAA GAACCTTAAG GAGGGTGGGC ATCGTGTACT CATCTTTTCC 
CAGATGACCA AGATGCTAGA CCTGCTAGAG GATTTCTTGG AACATGAAGG TTATAAATAC 
GAACGCATCG ATGGTGGAAT CACTGGGAAC ATGCGGCAAG AGGCCATTGA CCGCTTCAAT 
GCACCGGGTG CTCAGCAGTT CTGCTTCTTG CTTTCCACTC GAGCTGGGGG CCTTGGAATC 
AATCTGGCCA CTGCTGACAC AGTTATTATC TATGACTCTG ACTGGAACCC CCATAATGAC 
ATTCAGGCCT TTAGCAGAGC TCACCGGATT GGGCAAAATA AAAAGGTAAT GATCTACCGG 
TTTGTGACCC GTGCGTCAGT GGAGGAGCGC ATCACGCAGG TGGCAAAGAA GAAAATGATG 
CTGACGCATC TAGTGGTGCG GCCTGGGCTG GGCTCCAAGA CTGGATCTAT GTCCAAACAG 
GAGCTTGATG ATATCCTCAA ATTTGGCACT GAGGAACTAT TCAAGGATGA AGCCACTGAT 
GGAGGAGGAG ACAACAAAGA GGGAGAAGAT AGCAGTGTTA TCCACTACGA TGATAAGGCC 
ATTGAACGGC TGCTAGACCG TAACCAGGAT GAGACTGAAG ACACAGAATT GCAGGGCATG 
AATGAATATT TGAGCTCATT CAAAGTGGCC CAGTATGTGG TACGGGAAGA AGAAATGGGG 
GAGGAAGAGG AGGTAGAACG GGAAATCATT AAACAGGAAG AAAGTGTGGA TCCTGACTAC 
TGGGAGAAAT TGCTGCGGCA CCATTATGAG CAGCAGCAAG AAGATCTAGC CCGAAATCTG 
GGCAAAGGAA AAAGAATCCG TAAACAGGTC AACTACAATG ATGGCTCCCA GGAGGACCGA 
GGTGTGTGTG GCCGGCCCCG CCCCCCACCC ATGGGCCGTT CCACTAGAGC AGTGGGCCCC 
GCTCATCTGC CCTCTCTCCC TCCAGATTGG CAGGACGACC AGTCCGACAA CCAGTCCGAT 
TACTCAGTGG CTTCAGAGGA AGGTGATGAA GACTTTGATG AACGTTCAGA AGCTCCCCGT 
AGGCCCAGTC GTAAGGGCCT GCGGAATGAT AAAGATAAGC CATTGCCTCC TCTGTTGGCC 
CGTGTTGGTG GGAATATTGA AGTACTTGGT TTTAATGCTC GTCAGCGAAA AGCCTTTCTT 
AATGCAATTA TGCGATATGG TATGCCACCT CAGGATGCTT TTACTACCCA GTGGCTTGTA 
AGAGACCTGC GAGGCAAATC AGAGAAAGAG TTCAAGGCAT ATGTCTCTCT TTTCATGCGG 
CATTTATGTG AGCCGGGGGC AGATGGGGCT GAGACCTTTG CTGATGGTGT CCCCCGAGAA 
GGCCTGTCTC GCCAGCATGT CCTTACTAGA ATTGGTGTTA TGTCTTTGAT TCGCAAGAAG 
GTTCAGGAGT TTGAACATGT TAATGGGCGC TGGAGCATGC CTGAACTGGC TGAGGTGGAG 
GAAAACAAGA AGATGTCCCA GCCAGGGTCA CCCTCCCCAA AAACTCCTAC ACCCTCCACT 
CCAGGGGACA CGCAGCCCAA CACTCCTGCA CCTGTCCCAC CTGCTGAAGA TGGGATAAAA 
ATAGAGGAAA ATAGCCTCAA AGAAGAAGAG AGCATAGAAG GAGAAAAGGA GGTTAAATCT 
ACAGCCCCTG AGACTGCCAT TGAGTGTACA CAGGCCCCTG CCCCTGCCTC AGAGGATGAA 
AAGGTCGTTG TTGAACCCCC TGAGGGAGAG GAGAAAGTGG AAAAGGCAGA GGTGAAGGAG 
AGAACAGAGG AACCTATGGA GACAGAGCCC AAAGGTGCTG CTGATGTAGA GAAGGTGGAG 
GAAAAGTCAG CAATAGATCT GACCCCTATT GTGGTAGAAG ACAAAGAAGA GAAGAAAGAA 
GAAGAAGAGA AAAAAGAGGT GATGCTTCAG AATGGAGAGA CCCCCAAGGA CCTGAATGAT 
GAGAAACAGA AGAAAAATAT TAAACAACGT TTCATGTTTA ACATTGCAGA TGGTGGTTTT 
ACTGAGTTGC ACTCCCTTTG GCAGAATGAA GAGCGGGCAG CCACAGTTAC CAAGAAGACT 
TATGAGATCT GGCATCGACG GCATGACTAC TGGCTGCTAG CCGGCATTAT AAACCATGGC 
TATGCCCGGT GGCAAGACAT CCAGAATGAC CCACGCTATG CCATCCTCAA TGAGCCTTTC 
AAGGGTGAAA TGAACCGTGG CAATTTCTTA GAGATCAAGA ATAAATTTCT AGCTCGAAGG 
TTTAAGCTCT TAGAACAAGC TCTGGTGATT GAGGAACAGC TGCGCCGGGC TGCTTACTTG 
AACATGTCAG AAGACCCTTC TCACCCTTCC ATGGCCCTCA ACACCCGCTT TGCTGAGGTG 
GAGTGTTTGG CGGAAAGTCA TCAGCACCTG TCCAAGGAGT CAATGGCAGG AAACAAGCCA 
GCCAATGCAG TCCTGCACAA AGGTATTCTG AAACAGCTGG AAGAACTGCT GAGTGACATG 
AAAGCTGATG TGACTCGACT CCCAGCTACC ATTGCCCGAA TTCCCCCAGT TGCTGTGAGG 
TTACAGATGT CAGAGCGTAA CATTCTCAGC CGCCTGGCAA ACCGGGCACC CGAACCTACC 
CCACAGCAGG TAGCCCAGCA GCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024304572.1
CDS	219..6005
Translation	MASGLGSPSPCSAGSEEEDMDALLNNSLPPPHPENEEDPEEDLSETETPKLKKKKKPKKPRDPKIPKSKRQKKERMLLCRQLGDSSGEGPEFVEEEEEVALRSDSEGSDYTPGKKKKKKLGPKKEKKSKSKRKEEEEEEDDDDDSKEPKSSAQLLEDWGMEDIDHVFSEEDYRTLTNYKAFSQFVRPLIAAKNPKIAVSKMMMVLGAKWREFSTNNPFKGSSGASVAAAAAAAVAVVESMVTATEVAPPPPPVEVPIRKAKTKEGKGPNARRKPKGSPRVPDAKKPKPKKVAPLKIKLGGFGSKRKRSSSEDDDLDVESDFDDASINSYSVSDGSTSRSSRSRKKLRTTKKKKKDHQDYCEVCQQGGEIILCDTCPRAYHMVCLDPDMEKAPEGKWSCPHCEKEGIQWEAKEDNSEGEEILEEVGGDLEEEDDHHMEFCRVCKDGGELLCCDTCPSSYHIHCLNPPLPEIPNGEWLCPRCTCPALKGKVQKILIWKWGQPPSPTPVPRPPDADPNTPSPKPLEGRPERQFFVKWQGMSYWHCSWVSELQLELHCQVMFRNYQRKNDMDEPPSGDFGGDEEKSRKRKNKDPKFAEMEERFYRYGIKPEWMMIHRILNHSVDKKGHVHYLIKWRDLPYDQASWESEDVEIQDYDLFKQSYWNHRELMRGEEGRPGKKLKKVKLRKLERPPETPTVDPTVKYERQPEYLDATGGTLHPYQMEGLNWLRFSWAQGTDTILADEMGLGKTVQTAVFLYSLYKEGHSKGPFLVSAPLSTIINWEREFEMWAPDMYVVTYVGDKDSRAIIRENEFSFEDNAIRGGKKASRMKKEASVKFHVLLTSYELITIDMAILGSIDWACLIVDEAHRLKNNQSKFFRVLNGYSLQHKLLLTGTPLQNNLEELFHLLNFLTPERFHNLEGFLEEFADIAKEDQIKKLHDMLGPHMLRRLKADVFKNMPSKTELIVRVELSPMQKKYYKYILTRNFEALNARGGGNQVSLLNVVMDLKKCCNHPYLFPVAAMEAPKMPNGMYDGSALIRASGKLLLLQKMLKNLKEGGHRVLIFSQMTKMLDLLEDFLEHEGYKYERIDGGITGNMRQEAIDRFNAPGAQQFCFLLSTRAGGLGINLATADTVIIYDSDWNPHNDIQAFSRAHRIGQNKKVMIYRFVTRASVEERITQVAKKKMMLTHLVVRPGLGSKTGSMSKQELDDILKFGTEELFKDEATDGGGDNKEGEDSSVIHYDDKAIERLLDRNQDETEDTELQGMNEYLSSFKVAQYVVREEEMGEEEEVEREIIKQEESVDPDYWEKLLRHHYEQQQEDLARNLGKGKRIRKQVNYNDGSQEDRGVCGRPRPPPMGRSTRAVGPAHLPSLPPDWQDDQSDNQSDYSVASEEGDEDFDERSEAPRRPSRKGLRNDKDKPLPPLLARVGGNIEVLGFNARQRKAFLNAIMRYGMPPQDAFTTQWLVRDLRGKSEKEFKAYVSLFMRHLCEPGADGAETFADGVPREGLSRQHVLTRIGVMSLIRKKVQEFEHVNGRWSMPELAEVEENKKMSQPGSPSPKTPTPSTPGDTQPNTPAPVPPAEDGIKIEENSLKEEESIEGEKEVKSTAPETAIECTQAPAPASEDEKVVVEPPEGEEKVEKAEVKERTEEPMETEPKGAADVEKVEEKSAIDLTPIVVEDKEEKKEEEEKKEVMLQNGETPKDLNDEKQKKNIKQRFMFNIADGGFTELHSLWQNEERAATVTKKTYEIWHRRHDYWLLAGIINHGYARWQDIQNDPRYAILNEPFKGEMNRGNFLEIKNKFLARRFKLLEQALVIEEQLRRAAYLNMSEDPSHPSMALNTRFAEVECLAESHQHLSKESMAGNKPANAVLHKGILKQLEELLSDMKADVTRLPATIARIPPVAVRLQMSERNILSRLANRAPEPTPQQVAQQQ

Target ORF information:

RefSeq Version	XM_024448804.1
Organism	Homo sapiens(human)
Definition	Homo sapiens chromodomain helicase DNA binding protein 4 (CHD4), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024448804.1

ORF Insert Sequence:

ATGGCGTCGG GCCTGGGCTC CCCGTCCCCC TGCTCGGCGG GCAGTGAGGA GGAGGATATG 
GATGCACTTT TGAACAACAG CCTGCCCCCA CCCCACCCAG AAAATGAAGA GGACCCAGAA 
GAGGATTTGT CAGAAACAGA GACTCCAAAG CTCAAGAAGA AGAAAAAGCC TAAGAAACCT 
CGGGACCCTA AAATCCCTAA GAGCAAGCGC CAAAAAAAGG AGCGTATGCT CTTATGCCGG 
CAGCTGGGGG ACAGCTCTGG GGAGGGGCCA GAGTTTGTGG AGGAGGAGGA AGAGGTGGCT 
CTGCGCTCAG ACAGTGAGGG CAGCGACTAT ACTCCTGGCA AGAAGAAGAA GAAGAAGCTT 
GGACCTAAGA AAGAGAAGAA GAGCAAATCC AAGCGGAAGG AGGAGGAGGA GGAGGAGGAT 
GATGATGATG ATTCAAAGGA GCCTAAATCA TCTGCTCAGC TCCTGGAAGA CTGGGGCATG 
GAAGACATTG ACCACGTGTT CTCAGAGGAG GATTATCGAA CCCTCACCAA CTACAAGGCC 
TTCAGCCAGT TTGTCAGACC CCTCATTGCT GCCAAAAATC CCAAGATTGC TGTCTCCAAG 
ATGATGATGG TTTTGGGTGC AAAATGGCGG GAGTTCAGTA CCAATAACCC CTTCAAAGGC 
AGTTCTGGGG CATCAGTGGC AGCTGCGGCA GCAGCAGCGG TAGCTGTGGT GGAGAGCATG 
GTGACAGCCA CTGAGGTTGC ACCACCACCT CCCCCTGTGG AGGTGCCTAT CCGCAAGGCC 
AAGACCAAGG AGGGCAAAGG TCCCAATGCT CGGAGGAAGC CCAAGGGCAG CCCTCGTGTA 
CCTGATGCCA AGAAGCCTAA ACCCAAGAAA GTAGCTCCCC TGAAAATCAA GCTGGGAGGT 
TTTGGTTCCA AGCGTAAGAG ATCCTCGAGT GAGGATGATG ACTTAGATGT GGAATCTGAC 
TTCGATGATG CCAGTATCAA TAGCTATTCT GTTTCTGATG GTTCCACCAG CCGTAGTAGC 
CGCAGCCGCA AGAAACTCCG AACCACTAAA AAGAAAAAGA AAGACCACCA GGACTATTGC 
GAGGTGTGCC AGCAAGGCGG TGAGATCATC CTGTGTGATA CCTGTCCCCG TGCTTACCAC 
ATGGTCTGCC TGGATCCCGA CATGGAGAAG GCTCCCGAGG GCAAGTGGAG CTGCCCACAC 
TGCGAGAAGG AAGGCATCCA GTGGGAAGCT AAAGAGGACA ATTCGGAGGG TGAGGAGATC 
CTGGAAGAGG TTGGGGGAGA CCTCGAAGAG GAGGATGACC ACCATATGGA ATTCTGTCGG 
GTCTGCAAGG ATGGTGGGGA ACTGCTCTGC TGTGATACCT GTCCTTCTTC CTACCACATC 
CACTGCCTGA ATCCCCCACT TCCAGAGATC CCCAACGGTG AATGGCTCTG TCCCCGTTGT 
ACGTGTCCAG CTCTGAAGGG CAAAGTGCAG AAGATCCTAA TCTGGAAGTG GGGTCAGCCA 
CCATCTCCCA CACCAGTGCC TCGGCCTCCA GATGCTGATC CCAACACGCC CTCCCCAAAG 
CCCTTGGAGG GGCGGCCAGA GCGGCAGTTC TTTGTGAAAT GGCAAGGCAT GTCTTACTGG 
CACTGCTCCT GGGTTTCTGA ACTGCAGCTG GAGCTGCACT GTCAGGTGAT GTTCCGAAAC 
TATCAGCGGA AGAATGATAT GGATGAGCCA CCTTCTGGGG ACTTTGGTGG TGATGAAGAG 
AAAAGCCGAA AGCGAAAGAA CAAGGACCCT AAATTTGCAG AGATGGAGGA ACGCTTCTAT 
CGCTATGGGA TAAAACCCGA GTGGATGATG ATCCACCGAA TCCTCAACCA CAGTGTGGAC 
AAGAAGGGCC ACGTCCACTA CTTGATCAAG TGGCGGGACT TACCTTACGA TCAGGCTTCT 
TGGGAGAGTG AGGATGTGGA GATCCAGGAT TACGACCTGT TCAAGCAGAG CTATTGGAAT 
CACAGGGAGT TAATGAGGGG TGAGGAAGGC CGACCAGGCA AGAAGCTCAA GAAGGTGAAG 
CTTCGGAAGT TGGAGAGGCC TCCAGAAACG CCAACAGTTG ATCCAACAGT GAAGTATGAG 
CGACAGCCAG AGTACCTGGA TGCTACAGGT GGAACCCTGC ACCCCTATCA AATGGAGGGC 
CTGAATTGGT TGCGCTTCTC CTGGGCTCAG GGCACTGACA CCATCTTGGC TGATGAGATG 
GGCCTTGGGA AAACTGTACA GACAGCAGTC TTCCTGTATT CCCTTTACAA GGAGGGTCAT 
TCCAAAGGCC CCTTCCTAGT GAGCGCCCCT CTTTCTACCA TCATCAACTG GGAGCGGGAG 
TTTGAAATGT GGGCTCCAGA CATGTATGTC GTAACCTATG TGGGTGACAA GGACAGCCGT 
GCCATCATCC GAGAGAATGA GTTCTCCTTT GAAGACAATG CCATTCGTGG TGGCAAGAAG 
GCCTCCCGCA TGAAGAAAGA GGCATCTGTG AAATTCCATG TGCTGCTGAC ATCCTATGAA 
TTGATCACCA TTGACATGGC TATTTTGGGC TCTATTGATT GGGCCTGCCT CATCGTGGAT 
GAAGCCCATC GGCTGAAGAA CAATCAGTCT AAGTTCTTCC GGGTATTGAA TGGTTACTCA 
CTCCAGCACA AGCTGTTGCT GACTGGGACA CCATTACAAA ACAATCTGGA AGAGTTGTTT 
CATCTGCTCA ACTTTCTCAC CCCCGAGAGG TTCCACAATT TGGAAGGTTT TTTGGAGGAG 
TTTGCTGACA TTGCCAAGGA GGACCAGATA AAAAAACTGC ATGACATGCT GGGGCCGCAC 
ATGTTGCGGC GGCTCAAAGC CGATGTGTTC AAGAACATGC CCTCCAAGAC AGAACTAATT 
GTGCGTGTGG AGCTGAGCCC TATGCAGAAG AAATACTACA AGTACATCCT CACTCGAAAT 
TTTGAAGCAC TCAATGCCCG AGGTGGTGGC AACCAGGTGT CTCTGCTGAA TGTGGTGATG 
GATCTTAAGA AGTGCTGCAA CCATCCATAC CTCTTCCCTG TGGCTGCAAT GGAAGCTCCT 
AAGATGCCTA ATGGCATGTA TGATGGCAGT GCCCTAATCA GAGCATCTGG GAAATTATTG 
CTGCTGCAGA AAATGCTCAA GAACCTTAAG GAGGGTGGGC ATCGTGTACT CATCTTTTCC 
CAGATGACCA AGATGCTAGA CCTGCTAGAG GATTTCTTGG AACATGAAGG TTATAAATAC 
GAACGCATCG ATGGTGGAAT CACTGGGAAC ATGCGGCAAG AGGCCATTGA CCGCTTCAAT 
GCACCGGGTG CTCAGCAGTT CTGCTTCTTG CTTTCCACTC GAGCTGGGGG CCTTGGAATC 
AATCTGGCCA CTGCTGACAC AGTTATTATC TATGACTCTG ACTGGAACCC CCATAATGAC 
ATTCAGGCCT TTAGCAGAGC TCACCGGATT GGGCAAAATA AAAAGGTAAT GATCTACCGG 
TTTGTGACCC GTGCGTCAGT GGAGGAGCGC ATCACGCAGG TGGCAAAGAA GAAAATGATG 
CTGACGCATC TAGTGGTGCG GCCTGGGCTG GGCTCCAAGA CTGGATCTAT GTCCAAACAG 
GAGCTTGATG ATATCCTCAA ATTTGGCACT GAGGAACTAT TCAAGGATGA AGCCACTGAT 
GGAGGAGGAG ACAACAAAGA GGGAGAAGAT AGCAGTGTTA TCCACTACGA TGATAAGGCC 
ATTGAACGGC TGCTAGACCG TAACCAGGAT GAGACTGAAG ACACAGAATT GCAGGGCATG 
AATGAATATT TGAGCTCATT CAAAGTGGCC CAGTATGTGG TACGGGAAGA AGAAATGGGG 
GAGGAAGAGG AGGTAGAACG GGAAATCATT AAACAGGAAG AAAGTGTGGA TCCTGACTAC 
TGGGAGAAAT TGCTGCGGCA CCATTATGAG CAGCAGCAAG AAGATCTAGC CCGAAATCTG 
GGCAAAGGAA AAAGAATCCG TAAACAGGTC AACTACAATG ATGGCTCCCA GGAGGACCGA 
GGTGTGTGTG GCCGGCCCCG CCCCCCACCC ATGGGCCGTT CCACTAGAGC AGTGGGCCCC 
GCTCATCTGC CCTCTCTCCC TCCAGATTGG CAGGACGACC AGTCCGACAA CCAGTCCGAT 
TACTCAGTGG CTTCAGAGGA AGGTGATGAA GACTTTGATG AACGTTCAGA AGCTCCCCGT 
AGGCCCAGTC GTAAGGGCCT GCGGAATGAT AAAGATAAGC CATTGCCTCC TCTGTTGGCC 
CGTGTTGGTG GGAATATTGA AGTACTTGGT TTTAATGCTC GTCAGCGAAA AGCCTTTCTT 
AATGCAATTA TGCGATATGG TATGCCACCT CAGGATGCTT TTACTACCCA GTGGCTTGTA 
AGAGACCTGC GAGGCAAATC AGAGAAAGAG TTCAAGGCAT ATGTCTCTCT TTTCATGCGG 
CATTTATGTG AGCCGGGGGC AGATGGGGCT GAGACCTTTG CTGATGGTGT CCCCCGAGAA 
GGCCTGTCTC GCCAGCATGT CCTTACTAGA ATTGGTGTTA TGTCTTTGAT TCGCAAGAAG 
GTTCAGGAGT TTGAACATGT TAATGGGCGC TGGAGCATGC CTGAACTGGC TGAGGTGGAG 
GAAAACAAGA AGATGTCCCA GCCAGGGTCA CCCTCCCCAA AAACTCCTAC ACCCTCCACT 
CCAGGGGACA CGCAGCCCAA CACTCCTGCA CCTGTCCCAC CTGCTGAAGA TGGGATAAAA 
ATAGAGGAAA ATAGCCTCAA AGAAGAAGAG AGCATAGAAG GAGAAAAGGA GGTTAAATCT 
ACAGCCCCTG AGACTGCCAT TGAGTGTACA CAGGCCCCTG CCCCTGCCTC AGAGGATGAA 
AAGGTCGTTG TTGAACCCCC TGAGGGAGAG GAGAAAGTGG AAAAGGCAGA GGTGAAGGAG 
AGAACAGAGG AACCTATGGA GACAGAGCCC AAAGGTGCTG CTGATGTAGA GAAGGTGGAG 
GAAAAGTCAG CAATAGATCT GACCCCTATT GTGGTAGAAG ACAAAGAAGA GAAGAAAGAA 
GAAGAAGAGA AAAAAGAGGT GATGCTTCAG AATGGAGAGA CCCCCAAGGA CCTGAATGAT 
GAGAAACAGA AGAAAAATAT TAAACAACGT TTCATGTTTA ACATTGCAGA TGGTGGTTTT 
ACTGAGTTGC ACTCCCTTTG GCAGAATGAA GAGCGGGCAG CCACAGTTAC CAAGAAGACT 
TATGAGATCT GGCATCGACG GCATGACTAC TGGCTGCTAG CCGGCATTAT AAACCATGGC 
TATGCCCGGT GGCAAGACAT CCAGAATGAC CCACGCTATG CCATCCTCAA TGAGCCTTTC 
AAGGGTGAAA TGAACCGTGG CAATTTCTTA GAGATCAAGA ATAAATTTCT AGCTCGAAGG 
TTTAAGCTCT TAGAACAAGC TCTGGTGATT GAGGAACAGC TGCGCCGGGC TGCTTACTTG 
AACATGTCAG AAGACCCTTC TCACCCTTCC ATGGCCCTCA ACACCCGCTT TGCTGAGGTG 
GAGTGTTTGG CGGAAAGTCA TCAGCACCTG TCCAAGGAGT CAATGGCAGG AAACAAGCCA 
GCCAATGCAG TCCTGCACAA AGGTATTCTG AAACAGCTGG AAGAACTGCT GAGTGACATG 
AAAGCTGATG TGACTCGACT CCCAGCTACC ATTGCCCGAA TTCCCCCAGT TGCTGTGAGG 
TTACAGATGT CAGAGCGTAA CATTCTCAGC CGCCTGGCAA ACCGGGCACC CGAACCTACC 
CCACAGCAGG TAGCCCAGCA GCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHD4 ( XM_024448805.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024304573.1 Homo sapiens chromodomain helicase DNA binding protein 4 (CHD4), transcript variant X5, mRNA.

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CloneID	OHu103677
Clone ID Related Accession (Same CDS sequence)	XM_024448805.1
Accession Version	XM_024448805.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5763bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-25
Organism	Homo sapiens(human)
Product	chromodomain-helicase-DNA-binding protein 4 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCGG GCCTGGGCTC CCCGTCCCCC TGCTCGGCGG GCAGTGAGGA GGAGGATATG 
GATGCACTTT TGAACAACAG CCTGCCCCCA CCCCACCCAG AAAATGAAGA GGACCCAGAA 
GAGGATTTGT CAGAAACAGA GACTCCAAAG CTCAAGAAGA AGAAAAAGCC TAAGAAACCT 
CGGGACCCTA AAATCCCTAA GAGCAAGCGC CAAAAAAAGG AGCTGGGGGA CAGCTCTGGG 
GAGGGGCCAG AGTTTGTGGA GGAGGAGGAA GAGGTGGCTC TGCGCTCAGA CAGTGAGGGC 
AGCGACTATA CTCCTGGCAA GAAGAAGAAG AAGAAGCTTG GACCTAAGAA AGAGAAGAAG 
AGCAAATCCA AGCGGAAGGA GGAGGAGGAG GAGGAGGATG ATGATGATGA TTCAAAGGAG 
CCTAAATCAT CTGCTCAGCT CCTGGAAGAC TGGGGCATGG AAGACATTGA CCACGTGTTC 
TCAGAGGAGG ATTATCGAAC CCTCACCAAC TACAAGGCCT TCAGCCAGTT TGTCAGACCC 
CTCATTGCTG CCAAAAATCC CAAGATTGCT GTCTCCAAGA TGATGATGGT TTTGGGTGCA 
AAATGGCGGG AGTTCAGTAC CAATAACCCC TTCAAAGGCA GTTCTGGGGC ATCAGTGGCA 
GCTGCGGCAG CAGCAGCGGT AGCTGTGGTG GAGAGCATGG TGACAGCCAC TGAGGTTGCA 
CCACCACCTC CCCCTGTGGA GGTGCCTATC CGCAAGGCCA AGACCAAGGA GGGCAAAGGT 
CCCAATGCTC GGAGGAAGCC CAAGGGCAGC CCTCGTGTAC CTGATGCCAA GAAGCCTAAA 
CCCAAGAAAG TAGCTCCCCT GAAAATCAAG CTGGGAGGTT TTGGTTCCAA GCGTAAGAGA 
TCCTCGAGTG AGGATGATGA CTTAGATGTG GAATCTGACT TCGATGATGC CAGTATCAAT 
AGCTATTCTG TTTCTGATGG TTCCACCAGC CGTAGTAGCC GCAGCCGCAA GAAACTCCGA 
ACCACTAAAA AGAAAAAGAA AGACCACCAG GACTATTGCG AGGTGTGCCA GCAAGGCGGT 
GAGATCATCC TGTGTGATAC CTGTCCCCGT GCTTACCACA TGGTCTGCCT GGATCCCGAC 
ATGGAGAAGG CTCCCGAGGG CAAGTGGAGC TGCCCACACT GCGAGAAGGA AGGCATCCAG 
TGGGAAGCTA AAGAGGACAA TTCGGAGGGT GAGGAGATCC TGGAAGAGGT TGGGGGAGAC 
CTCGAAGAGG AGGATGACCA CCATATGGAA TTCTGTCGGG TCTGCAAGGA TGGTGGGGAA 
CTGCTCTGCT GTGATACCTG TCCTTCTTCC TACCACATCC ACTGCCTGAA TCCCCCACTT 
CCAGAGATCC CCAACGGTGA ATGGCTCTGT CCCCGTTGTA CGTGTCCAGC TCTGAAGGGC 
AAAGTGCAGA AGATCCTAAT CTGGAAGTGG GGTCAGCCAC CATCTCCCAC ACCAGTGCCT 
CGGCCTCCAG ATGCTGATCC CAACACGCCC TCCCCAAAGC CCTTGGAGGG GCGGCCAGAG 
CGGCAGTTCT TTGTGAAATG GCAAGGCATG TCTTACTGGC ACTGCTCCTG GGTTTCTGAA 
CTGCAGCTGG AGCTGCACTG TCAGGTGATG TTCCGAAACT ATCAGCGGAA GAATGATATG 
GATGAGCCAC CTTCTGGGGA CTTTGGTGGT GATGAAGAGA AAAGCCGAAA GCGAAAGAAC 
AAGGACCCTA AATTTGCAGA GATGGAGGAA CGCTTCTATC GCTATGGGAT AAAACCCGAG 
TGGATGATGA TCCACCGAAT CCTCAACCAC AGTGTGGACA AGAAGGGCCA CGTCCACTAC 
TTGATCAAGT GGCGGGACTT ACCTTACGAT CAGGCTTCTT GGGAGAGTGA GGATGTGGAG 
ATCCAGGATT ACGACCTGTT CAAGCAGAGC TATTGGAATC ACAGGGAGTT AATGAGGGGT 
GAGGAAGGCC GACCAGGCAA GAAGCTCAAG AAGGTGAAGC TTCGGAAGTT GGAGAGGCCT 
CCAGAAACGC CAACAGTTGA TCCAACAGTG AAGTATGAGC GACAGCCAGA GTACCTGGAT 
GCTACAGGTG GAACCCTGCA CCCCTATCAA ATGGAGGGCC TGAATTGGTT GCGCTTCTCC 
TGGGCTCAGG GCACTGACAC CATCTTGGCT GATGAGATGG GCCTTGGGAA AACTGTACAG 
ACAGCAGTCT TCCTGTATTC CCTTTACAAG GAGGGTCATT CCAAAGGCCC CTTCCTAGTG 
AGCGCCCCTC TTTCTACCAT CATCAACTGG GAGCGGGAGT TTGAAATGTG GGCTCCAGAC 
ATGTATGTCG TAACCTATGT GGGTGACAAG GACAGCCGTG CCATCATCCG AGAGAATGAG 
TTCTCCTTTG AAGACAATGC CATTCGTGGT GGCAAGAAGG CCTCCCGCAT GAAGAAAGAG 
GCATCTGTGA AATTCCATGT GCTGCTGACA TCCTATGAAT TGATCACCAT TGACATGGCT 
ATTTTGGGCT CTATTGATTG GGCCTGCCTC ATCGTGGATG AAGCCCATCG GCTGAAGAAC 
AATCAGTCTA AGTTCTTCCG GGTATTGAAT GGTTACTCAC TCCAGCACAA GCTGTTGCTG 
ACTGGGACAC CATTACAAAA CAATCTGGAA GAGTTGTTTC ATCTGCTCAA CTTTCTCACC 
CCCGAGAGGT TCCACAATTT GGAAGGTTTT TTGGAGGAGT TTGCTGACAT TGCCAAGGAG 
GACCAGATAA AAAAACTGCA TGACATGCTG GGGCCGCACA TGTTGCGGCG GCTCAAAGCC 
GATGTGTTCA AGAACATGCC CTCCAAGACA GAACTAATTG TGCGTGTGGA GCTGAGCCCT 
ATGCAGAAGA AATACTACAA GTACATCCTC ACTCGAAATT TTGAAGCACT CAATGCCCGA 
GGTGGTGGCA ACCAGGTGTC TCTGCTGAAT GTGGTGATGG ATCTTAAGAA GTGCTGCAAC 
CATCCATACC TCTTCCCTGT GGCTGCAATG GAAGCTCCTA AGATGCCTAA TGGCATGTAT 
GATGGCAGTG CCCTAATCAG AGCATCTGGG AAATTATTGC TGCTGCAGAA AATGCTCAAG 
AACCTTAAGG AGGGTGGGCA TCGTGTACTC ATCTTTTCCC AGATGACCAA GATGCTAGAC 
CTGCTAGAGG ATTTCTTGGA ACATGAAGGT TATAAATACG AACGCATCGA TGGTGGAATC 
ACTGGGAACA TGCGGCAAGA GGCCATTGAC CGCTTCAATG CACCGGGTGC TCAGCAGTTC 
TGCTTCTTGC TTTCCACTCG AGCTGGGGGC CTTGGAATCA ATCTGGCCAC TGCTGACACA 
GTTATTATCT ATGACTCTGA CTGGAACCCC CATAATGACA TTCAGGCCTT TAGCAGAGCT 
CACCGGATTG GGCAAAATAA AAAGGTAATG ATCTACCGGT TTGTGACCCG TGCGTCAGTG 
GAGGAGCGCA TCACGCAGGT GGCAAAGAAG AAAATGATGC TGACGCATCT AGTGGTGCGG 
CCTGGGCTGG GCTCCAAGAC TGGATCTATG TCCAAACAGG AGCTTGATGA TATCCTCAAA 
TTTGGCACTG AGGAACTATT CAAGGATGAA GCCACTGATG GAGGAGGAGA CAACAAAGAG 
GGAGAAGATA GCAGTGTTAT CCACTACGAT GATAAGGCCA TTGAACGGCT GCTAGACCGT 
AACCAGGATG AGACTGAAGA CACAGAATTG CAGGGCATGA ATGAATATTT GAGCTCATTC 
AAAGTGGCCC AGTATGTGGT ACGGGAAGAA GAAATGGGGG AGGAAGAGGA GGTAGAACGG 
GAAATCATTA AACAGGAAGA AAGTGTGGAT CCTGACTACT GGGAGAAATT GCTGCGGCAC 
CATTATGAGC AGCAGCAAGA AGATCTAGCC CGAAATCTGG GCAAAGGAAA AAGAATCCGT 
AAACAGGTCA ACTACAATGA TGGCTCCCAG GAGGACCGAG GTGTGTGTGG CCGGCCCCGC 
CCCCCACCCA TGGGCCGTTC CACTAGAGCA GTGGGCCCCG CTCATCTGCC CTCTCTCCCT 
CCAGATTGGC AGGACGACCA GTCCGACAAC CAGTCCGATT ACTCAGTGGC TTCAGAGGAA 
GGTGATGAAG ACTTTGATGA ACGTTCAGAA GCTCCCCGTA GGCCCAGTCG TAAGGGCCTG 
CGGAATGATA AAGATAAGCC ATTGCCTCCT CTGTTGGCCC GTGTTGGTGG GAATATTGAA 
GTACTTGGTT TTAATGCTCG TCAGCGAAAA GCCTTTCTTA ATGCAATTAT GCGATATGGT 
ATGCCACCTC AGGATGCTTT TACTACCCAG TGGCTTGTAA GAGACCTGCG AGGCAAATCA 
GAGAAAGAGT TCAAGGCATA TGTCTCTCTT TTCATGCGGC ATTTATGTGA GCCGGGGGCA 
GATGGGGCTG AGACCTTTGC TGATGGTGTC CCCCGAGAAG GCCTGTCTCG CCAGCATGTC 
CTTACTAGAA TTGGTGTTAT GTCTTTGATT CGCAAGAAGG TTCAGGAGTT TGAACATGTT 
AATGGGCGCT GGAGCATGCC TGAACTGGCT GAGGTGGAGG AAAACAAGAA GATGTCCCAG 
CCAGGGTCAC CCTCCCCAAA AACTCCTACA CCCTCCACTC CAGGGGACAC GCAGCCCAAC 
ACTCCTGCAC CTGTCCCACC TGCTGAAGAT GGGATAAAAA TAGAGGAAAA TAGCCTCAAA 
GAAGAAGAGA GCATAGAAGG AGAAAAGGAG GTTAAATCTA CAGCCCCTGA GACTGCCATT 
GAGTGTACAC AGGCCCCTGC CCCTGCCTCA GAGGATGAAA AGGTCGTTGT TGAACCCCCT 
GAGGGAGAGG AGAAAGTGGA AAAGGCAGAG GTGAAGGAGA GAACAGAGGA ACCTATGGAG 
ACAGAGCCCA AAGGTGCTGC TGATGTAGAG AAGGTGGAGG AAAAGTCAGC AATAGATCTG 
ACCCCTATTG TGGTAGAAGA CAAAGAAGAG AAGAAAGAAG AAGAAGAGAA AAAAGAGGTG 
ATGCTTCAGA ATGGAGAGAC CCCCAAGGAC CTGAATGATG AGAAACAGAA GAAAAATATT 
AAACAACGTT TCATGTTTAA CATTGCAGAT GGTGGTTTTA CTGAGTTGCA CTCCCTTTGG 
CAGAATGAAG AGCGGGCAGC CACAGTTACC AAGAAGACTT ATGAGATCTG GCATCGACGG 
CATGACTACT GGCTGCTAGC CGGCATTATA AACCATGGCT ATGCCCGGTG GCAAGACATC 
CAGAATGACC CACGCTATGC CATCCTCAAT GAGCCTTTCA AGGGTGAAAT GAACCGTGGC 
AATTTCTTAG AGATCAAGAA TAAATTTCTA GCTCGAAGGT TTAAGCTCTT AGAACAAGCT 
CTGGTGATTG AGGAACAGCT GCGCCGGGCT GCTTACTTGA ACATGTCAGA AGACCCTTCT 
CACCCTTCCA TGGCCCTCAA CACCCGCTTT GCTGAGGTGG AGTGTTTGGC GGAAAGTCAT 
CAGCACCTGT CCAAGGAGTC AATGGCAGGA AACAAGCCAG CCAATGCAGT CCTGCACAAA 
GTTCTGAAAC AGCTGGAAGA ACTGCTGAGT GACATGAAAG CTGATGTGAC TCGACTCCCA 
GCTACCATTG CCCGAATTCC CCCAGTTGCT GTGAGGTTAC AGATGTCAGA GCGTAACATT 
CTCAGCCGCC TGGCAAACCG GGCACCCGAA CCTACCCCAC AGCAGGTAGC CCAGCAGCAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024304573.1
CDS	165..5927
Translation	MASGLGSPSPCSAGSEEEDMDALLNNSLPPPHPENEEDPEEDLSETETPKLKKKKKPKKPRDPKIPKSKRQKKELGDSSGEGPEFVEEEEEVALRSDSEGSDYTPGKKKKKKLGPKKEKKSKSKRKEEEEEEDDDDDSKEPKSSAQLLEDWGMEDIDHVFSEEDYRTLTNYKAFSQFVRPLIAAKNPKIAVSKMMMVLGAKWREFSTNNPFKGSSGASVAAAAAAAVAVVESMVTATEVAPPPPPVEVPIRKAKTKEGKGPNARRKPKGSPRVPDAKKPKPKKVAPLKIKLGGFGSKRKRSSSEDDDLDVESDFDDASINSYSVSDGSTSRSSRSRKKLRTTKKKKKDHQDYCEVCQQGGEIILCDTCPRAYHMVCLDPDMEKAPEGKWSCPHCEKEGIQWEAKEDNSEGEEILEEVGGDLEEEDDHHMEFCRVCKDGGELLCCDTCPSSYHIHCLNPPLPEIPNGEWLCPRCTCPALKGKVQKILIWKWGQPPSPTPVPRPPDADPNTPSPKPLEGRPERQFFVKWQGMSYWHCSWVSELQLELHCQVMFRNYQRKNDMDEPPSGDFGGDEEKSRKRKNKDPKFAEMEERFYRYGIKPEWMMIHRILNHSVDKKGHVHYLIKWRDLPYDQASWESEDVEIQDYDLFKQSYWNHRELMRGEEGRPGKKLKKVKLRKLERPPETPTVDPTVKYERQPEYLDATGGTLHPYQMEGLNWLRFSWAQGTDTILADEMGLGKTVQTAVFLYSLYKEGHSKGPFLVSAPLSTIINWEREFEMWAPDMYVVTYVGDKDSRAIIRENEFSFEDNAIRGGKKASRMKKEASVKFHVLLTSYELITIDMAILGSIDWACLIVDEAHRLKNNQSKFFRVLNGYSLQHKLLLTGTPLQNNLEELFHLLNFLTPERFHNLEGFLEEFADIAKEDQIKKLHDMLGPHMLRRLKADVFKNMPSKTELIVRVELSPMQKKYYKYILTRNFEALNARGGGNQVSLLNVVMDLKKCCNHPYLFPVAAMEAPKMPNGMYDGSALIRASGKLLLLQKMLKNLKEGGHRVLIFSQMTKMLDLLEDFLEHEGYKYERIDGGITGNMRQEAIDRFNAPGAQQFCFLLSTRAGGLGINLATADTVIIYDSDWNPHNDIQAFSRAHRIGQNKKVMIYRFVTRASVEERITQVAKKKMMLTHLVVRPGLGSKTGSMSKQELDDILKFGTEELFKDEATDGGGDNKEGEDSSVIHYDDKAIERLLDRNQDETEDTELQGMNEYLSSFKVAQYVVREEEMGEEEEVEREIIKQEESVDPDYWEKLLRHHYEQQQEDLARNLGKGKRIRKQVNYNDGSQEDRGVCGRPRPPPMGRSTRAVGPAHLPSLPPDWQDDQSDNQSDYSVASEEGDEDFDERSEAPRRPSRKGLRNDKDKPLPPLLARVGGNIEVLGFNARQRKAFLNAIMRYGMPPQDAFTTQWLVRDLRGKSEKEFKAYVSLFMRHLCEPGADGAETFADGVPREGLSRQHVLTRIGVMSLIRKKVQEFEHVNGRWSMPELAEVEENKKMSQPGSPSPKTPTPSTPGDTQPNTPAPVPPAEDGIKIEENSLKEEESIEGEKEVKSTAPETAIECTQAPAPASEDEKVVVEPPEGEEKVEKAEVKERTEEPMETEPKGAADVEKVEEKSAIDLTPIVVEDKEEKKEEEEKKEVMLQNGETPKDLNDEKQKKNIKQRFMFNIADGGFTELHSLWQNEERAATVTKKTYEIWHRRHDYWLLAGIINHGYARWQDIQNDPRYAILNEPFKGEMNRGNFLEIKNKFLARRFKLLEQALVIEEQLRRAAYLNMSEDPSHPSMALNTRFAEVECLAESHQHLSKESMAGNKPANAVLHKVLKQLEELLSDMKADVTRLPATIARIPPVAVRLQMSERNILSRLANRAPEPTPQQVAQQQ

Target ORF information:

RefSeq Version	XM_024448805.1
Organism	Homo sapiens(human)
Definition	Homo sapiens chromodomain helicase DNA binding protein 4 (CHD4), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024448805.1

ORF Insert Sequence:

ATGGCGTCGG GCCTGGGCTC CCCGTCCCCC TGCTCGGCGG GCAGTGAGGA GGAGGATATG 
GATGCACTTT TGAACAACAG CCTGCCCCCA CCCCACCCAG AAAATGAAGA GGACCCAGAA 
GAGGATTTGT CAGAAACAGA GACTCCAAAG CTCAAGAAGA AGAAAAAGCC TAAGAAACCT 
CGGGACCCTA AAATCCCTAA GAGCAAGCGC CAAAAAAAGG AGCTGGGGGA CAGCTCTGGG 
GAGGGGCCAG AGTTTGTGGA GGAGGAGGAA GAGGTGGCTC TGCGCTCAGA CAGTGAGGGC 
AGCGACTATA CTCCTGGCAA GAAGAAGAAG AAGAAGCTTG GACCTAAGAA AGAGAAGAAG 
AGCAAATCCA AGCGGAAGGA GGAGGAGGAG GAGGAGGATG ATGATGATGA TTCAAAGGAG 
CCTAAATCAT CTGCTCAGCT CCTGGAAGAC TGGGGCATGG AAGACATTGA CCACGTGTTC 
TCAGAGGAGG ATTATCGAAC CCTCACCAAC TACAAGGCCT TCAGCCAGTT TGTCAGACCC 
CTCATTGCTG CCAAAAATCC CAAGATTGCT GTCTCCAAGA TGATGATGGT TTTGGGTGCA 
AAATGGCGGG AGTTCAGTAC CAATAACCCC TTCAAAGGCA GTTCTGGGGC ATCAGTGGCA 
GCTGCGGCAG CAGCAGCGGT AGCTGTGGTG GAGAGCATGG TGACAGCCAC TGAGGTTGCA 
CCACCACCTC CCCCTGTGGA GGTGCCTATC CGCAAGGCCA AGACCAAGGA GGGCAAAGGT 
CCCAATGCTC GGAGGAAGCC CAAGGGCAGC CCTCGTGTAC CTGATGCCAA GAAGCCTAAA 
CCCAAGAAAG TAGCTCCCCT GAAAATCAAG CTGGGAGGTT TTGGTTCCAA GCGTAAGAGA 
TCCTCGAGTG AGGATGATGA CTTAGATGTG GAATCTGACT TCGATGATGC CAGTATCAAT 
AGCTATTCTG TTTCTGATGG TTCCACCAGC CGTAGTAGCC GCAGCCGCAA GAAACTCCGA 
ACCACTAAAA AGAAAAAGAA AGACCACCAG GACTATTGCG AGGTGTGCCA GCAAGGCGGT 
GAGATCATCC TGTGTGATAC CTGTCCCCGT GCTTACCACA TGGTCTGCCT GGATCCCGAC 
ATGGAGAAGG CTCCCGAGGG CAAGTGGAGC TGCCCACACT GCGAGAAGGA AGGCATCCAG 
TGGGAAGCTA AAGAGGACAA TTCGGAGGGT GAGGAGATCC TGGAAGAGGT TGGGGGAGAC 
CTCGAAGAGG AGGATGACCA CCATATGGAA TTCTGTCGGG TCTGCAAGGA TGGTGGGGAA 
CTGCTCTGCT GTGATACCTG TCCTTCTTCC TACCACATCC ACTGCCTGAA TCCCCCACTT 
CCAGAGATCC CCAACGGTGA ATGGCTCTGT CCCCGTTGTA CGTGTCCAGC TCTGAAGGGC 
AAAGTGCAGA AGATCCTAAT CTGGAAGTGG GGTCAGCCAC CATCTCCCAC ACCAGTGCCT 
CGGCCTCCAG ATGCTGATCC CAACACGCCC TCCCCAAAGC CCTTGGAGGG GCGGCCAGAG 
CGGCAGTTCT TTGTGAAATG GCAAGGCATG TCTTACTGGC ACTGCTCCTG GGTTTCTGAA 
CTGCAGCTGG AGCTGCACTG TCAGGTGATG TTCCGAAACT ATCAGCGGAA GAATGATATG 
GATGAGCCAC CTTCTGGGGA CTTTGGTGGT GATGAAGAGA AAAGCCGAAA GCGAAAGAAC 
AAGGACCCTA AATTTGCAGA GATGGAGGAA CGCTTCTATC GCTATGGGAT AAAACCCGAG 
TGGATGATGA TCCACCGAAT CCTCAACCAC AGTGTGGACA AGAAGGGCCA CGTCCACTAC 
TTGATCAAGT GGCGGGACTT ACCTTACGAT CAGGCTTCTT GGGAGAGTGA GGATGTGGAG 
ATCCAGGATT ACGACCTGTT CAAGCAGAGC TATTGGAATC ACAGGGAGTT AATGAGGGGT 
GAGGAAGGCC GACCAGGCAA GAAGCTCAAG AAGGTGAAGC TTCGGAAGTT GGAGAGGCCT 
CCAGAAACGC CAACAGTTGA TCCAACAGTG AAGTATGAGC GACAGCCAGA GTACCTGGAT 
GCTACAGGTG GAACCCTGCA CCCCTATCAA ATGGAGGGCC TGAATTGGTT GCGCTTCTCC 
TGGGCTCAGG GCACTGACAC CATCTTGGCT GATGAGATGG GCCTTGGGAA AACTGTACAG 
ACAGCAGTCT TCCTGTATTC CCTTTACAAG GAGGGTCATT CCAAAGGCCC CTTCCTAGTG 
AGCGCCCCTC TTTCTACCAT CATCAACTGG GAGCGGGAGT TTGAAATGTG GGCTCCAGAC 
ATGTATGTCG TAACCTATGT GGGTGACAAG GACAGCCGTG CCATCATCCG AGAGAATGAG 
TTCTCCTTTG AAGACAATGC CATTCGTGGT GGCAAGAAGG CCTCCCGCAT GAAGAAAGAG 
GCATCTGTGA AATTCCATGT GCTGCTGACA TCCTATGAAT TGATCACCAT TGACATGGCT 
ATTTTGGGCT CTATTGATTG GGCCTGCCTC ATCGTGGATG AAGCCCATCG GCTGAAGAAC 
AATCAGTCTA AGTTCTTCCG GGTATTGAAT GGTTACTCAC TCCAGCACAA GCTGTTGCTG 
ACTGGGACAC CATTACAAAA CAATCTGGAA GAGTTGTTTC ATCTGCTCAA CTTTCTCACC 
CCCGAGAGGT TCCACAATTT GGAAGGTTTT TTGGAGGAGT TTGCTGACAT TGCCAAGGAG 
GACCAGATAA AAAAACTGCA TGACATGCTG GGGCCGCACA TGTTGCGGCG GCTCAAAGCC 
GATGTGTTCA AGAACATGCC CTCCAAGACA GAACTAATTG TGCGTGTGGA GCTGAGCCCT 
ATGCAGAAGA AATACTACAA GTACATCCTC ACTCGAAATT TTGAAGCACT CAATGCCCGA 
GGTGGTGGCA ACCAGGTGTC TCTGCTGAAT GTGGTGATGG ATCTTAAGAA GTGCTGCAAC 
CATCCATACC TCTTCCCTGT GGCTGCAATG GAAGCTCCTA AGATGCCTAA TGGCATGTAT 
GATGGCAGTG CCCTAATCAG AGCATCTGGG AAATTATTGC TGCTGCAGAA AATGCTCAAG 
AACCTTAAGG AGGGTGGGCA TCGTGTACTC ATCTTTTCCC AGATGACCAA GATGCTAGAC 
CTGCTAGAGG ATTTCTTGGA ACATGAAGGT TATAAATACG AACGCATCGA TGGTGGAATC 
ACTGGGAACA TGCGGCAAGA GGCCATTGAC CGCTTCAATG CACCGGGTGC TCAGCAGTTC 
TGCTTCTTGC TTTCCACTCG AGCTGGGGGC CTTGGAATCA ATCTGGCCAC TGCTGACACA 
GTTATTATCT ATGACTCTGA CTGGAACCCC CATAATGACA TTCAGGCCTT TAGCAGAGCT 
CACCGGATTG GGCAAAATAA AAAGGTAATG ATCTACCGGT TTGTGACCCG TGCGTCAGTG 
GAGGAGCGCA TCACGCAGGT GGCAAAGAAG AAAATGATGC TGACGCATCT AGTGGTGCGG 
CCTGGGCTGG GCTCCAAGAC TGGATCTATG TCCAAACAGG AGCTTGATGA TATCCTCAAA 
TTTGGCACTG AGGAACTATT CAAGGATGAA GCCACTGATG GAGGAGGAGA CAACAAAGAG 
GGAGAAGATA GCAGTGTTAT CCACTACGAT GATAAGGCCA TTGAACGGCT GCTAGACCGT 
AACCAGGATG AGACTGAAGA CACAGAATTG CAGGGCATGA ATGAATATTT GAGCTCATTC 
AAAGTGGCCC AGTATGTGGT ACGGGAAGAA GAAATGGGGG AGGAAGAGGA GGTAGAACGG 
GAAATCATTA AACAGGAAGA AAGTGTGGAT CCTGACTACT GGGAGAAATT GCTGCGGCAC 
CATTATGAGC AGCAGCAAGA AGATCTAGCC CGAAATCTGG GCAAAGGAAA AAGAATCCGT 
AAACAGGTCA ACTACAATGA TGGCTCCCAG GAGGACCGAG GTGTGTGTGG CCGGCCCCGC 
CCCCCACCCA TGGGCCGTTC CACTAGAGCA GTGGGCCCCG CTCATCTGCC CTCTCTCCCT 
CCAGATTGGC AGGACGACCA GTCCGACAAC CAGTCCGATT ACTCAGTGGC TTCAGAGGAA 
GGTGATGAAG ACTTTGATGA ACGTTCAGAA GCTCCCCGTA GGCCCAGTCG TAAGGGCCTG 
CGGAATGATA AAGATAAGCC ATTGCCTCCT CTGTTGGCCC GTGTTGGTGG GAATATTGAA 
GTACTTGGTT TTAATGCTCG TCAGCGAAAA GCCTTTCTTA ATGCAATTAT GCGATATGGT 
ATGCCACCTC AGGATGCTTT TACTACCCAG TGGCTTGTAA GAGACCTGCG AGGCAAATCA 
GAGAAAGAGT TCAAGGCATA TGTCTCTCTT TTCATGCGGC ATTTATGTGA GCCGGGGGCA 
GATGGGGCTG AGACCTTTGC TGATGGTGTC CCCCGAGAAG GCCTGTCTCG CCAGCATGTC 
CTTACTAGAA TTGGTGTTAT GTCTTTGATT CGCAAGAAGG TTCAGGAGTT TGAACATGTT 
AATGGGCGCT GGAGCATGCC TGAACTGGCT GAGGTGGAGG AAAACAAGAA GATGTCCCAG 
CCAGGGTCAC CCTCCCCAAA AACTCCTACA CCCTCCACTC CAGGGGACAC GCAGCCCAAC 
ACTCCTGCAC CTGTCCCACC TGCTGAAGAT GGGATAAAAA TAGAGGAAAA TAGCCTCAAA 
GAAGAAGAGA GCATAGAAGG AGAAAAGGAG GTTAAATCTA CAGCCCCTGA GACTGCCATT 
GAGTGTACAC AGGCCCCTGC CCCTGCCTCA GAGGATGAAA AGGTCGTTGT TGAACCCCCT 
GAGGGAGAGG AGAAAGTGGA AAAGGCAGAG GTGAAGGAGA GAACAGAGGA ACCTATGGAG 
ACAGAGCCCA AAGGTGCTGC TGATGTAGAG AAGGTGGAGG AAAAGTCAGC AATAGATCTG 
ACCCCTATTG TGGTAGAAGA CAAAGAAGAG AAGAAAGAAG AAGAAGAGAA AAAAGAGGTG 
ATGCTTCAGA ATGGAGAGAC CCCCAAGGAC CTGAATGATG AGAAACAGAA GAAAAATATT 
AAACAACGTT TCATGTTTAA CATTGCAGAT GGTGGTTTTA CTGAGTTGCA CTCCCTTTGG 
CAGAATGAAG AGCGGGCAGC CACAGTTACC AAGAAGACTT ATGAGATCTG GCATCGACGG 
CATGACTACT GGCTGCTAGC CGGCATTATA AACCATGGCT ATGCCCGGTG GCAAGACATC 
CAGAATGACC CACGCTATGC CATCCTCAAT GAGCCTTTCA AGGGTGAAAT GAACCGTGGC 
AATTTCTTAG AGATCAAGAA TAAATTTCTA GCTCGAAGGT TTAAGCTCTT AGAACAAGCT 
CTGGTGATTG AGGAACAGCT GCGCCGGGCT GCTTACTTGA ACATGTCAGA AGACCCTTCT 
CACCCTTCCA TGGCCCTCAA CACCCGCTTT GCTGAGGTGG AGTGTTTGGC GGAAAGTCAT 
CAGCACCTGT CCAAGGAGTC AATGGCAGGA AACAAGCCAG CCAATGCAGT CCTGCACAAA 
GTTCTGAAAC AGCTGGAAGA ACTGCTGAGT GACATGAAAG CTGATGTGAC TCGACTCCCA 
GCTACCATTG CCCGAATTCC CCCAGTTGCT GTGAGGTTAC AGATGTCAGA GCGTAACATT 
CTCAGCCGCC TGGCAAACCG GGCACCCGAA CCTACCCCAC AGCAGGTAGC CCAGCAGCAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHD4 ( NM_001297553.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001284482.1 Homo sapiens chromodomain helicase DNA binding protein 4 (CHD4), transcript variant 2, mRNA.

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CloneID	OHu28781
Clone ID Related Accession (Same CDS sequence)	NM_001297553.1 , NM_001297553.2 , XM_005253668.3
Accession Version	NM_001297553.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5718bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-27
Organism	Homo sapiens(human)
Product	chromodomain-helicase-DNA-binding protein 4 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from X86691.1, CR936680.1, AC006064.10 and BC038596.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CR936680.1, SRR1803611.101199.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGGCGTCGG GCCTGGGCTC CCCGTCCCCC TGCTCGGCGG GCAGTGAGGA GGAGGATATG 
GATGCACTTT TGAACAACAG CCTGCCCCCA CCCCACCCAG AAAATGAAGA GGACCCAGAA 
GAGGATTTGT CAGAAACAGA GACTCCAAAG CTCAAGAAGA AGAAAAAGCC TAAGAAACCT 
CGGGACCCTA AAATCCCTAA GAGCAAGCGC CAAAAAAAGG AGCTGGGGGA CAGCTCTGGG 
GAGGGGCCAG AGTTTGTGGA GGAGGAGGAA GAGGTGGCTC TGCGCTCAGA CAGTGAGGGC 
AGCGACTATA CTCCTGGCAA GAAGAAGAAG AAGAAGCTTG GACCTAAGAA AGAGAAGAAG 
AGCAAATCCA AGCGGAAGGA GGAGGAGGAG GAGGAGGATG ATGATGATGA TTCAAAGGAG 
CCTAAATCAT CTGCTCAGCT CCTGGAAGAC TGGGGCATGG AAGACATTGA CCACGTGTTC 
TCAGAGGAGG ATTATCGAAC CCTCACCAAC TACAAGGCCT TCAGCCAGTT TGTCAGACCC 
CTCATTGCTG CCAAAAATCC CAAGATTGCT GTCTCCAAGA TGATGATGGT TTTGGGTGCA 
AAATGGCGGG AGTTCAGTAC CAATAACCCC TTCAAAGGCA GTTCTGGGGC ATCAGTGGCA 
GCTGCGGCAG CAGCAGCGGT AGCTGTGGTG GAGAGCATGG TGACAGCCAC TGAGGTTGCA 
CCACCACCTC CCCCTGTGGA GGTGCCTATC CGCAAGGCCA AGACCAAGGA GGGCAAAGGT 
CCCAATGCTC GGAGGAAGCC CAAGGGCAGC CCTCGTGTAC CTGATGCCAA GAAGCCTAAA 
CCCAAGAAAG TAGCTCCCCT GAAAATCAAG CTGGGAGGTT TTGGTTCCAA GCGTAAGAGA 
TCCTCGAGTG AGGATGATGA CTTAGATGTG GAATCTGACT TCGATGATGC CAGTATCAAT 
AGCTATTCTG TTTCTGATGG TTCCACCAGC CGTAGTAGCC GCAGCCGCAA GAAACTCCGA 
ACCACTAAAA AGAAAAAGAA AGGCGAGGAG GAGGTGACTG CTGTGGATGG TTATGAGACA 
GACCACCAGG ACTATTGCGA GGTGTGCCAG CAAGGCGGTG AGATCATCCT GTGTGATACC 
TGTCCCCGTG CTTACCACAT GGTCTGCCTG GATCCCGACA TGGAGAAGGC TCCCGAGGGC 
AAGTGGAGCT GCCCACACTG CGAGAAGGAA GGCATCCAGT GGGAAGCTAA AGAGGACAAT 
TCGGAGGGTG AGGAGATCCT GGAAGAGGTT GGGGGAGACC TCGAAGAGGA GGATGACCAC 
CATATGGAAT TCTGTCGGGT CTGCAAGGAT GGTGGGGAAC TGCTCTGCTG TGATACCTGT 
CCTTCTTCCT ACCACATCCA CTGCCTGAAT CCCCCACTTC CAGAGATCCC CAACGGTGAA 
TGGCTCTGTC CCCGTTGTAC GTGTCCAGCT CTGAAGGGCA AAGTGCAGAA GATCCTAATC 
TGGAAGTGGG GTCAGCCACC ATCTCCCACA CCAGTGCCTC GGCCTCCAGA TGCTGATCCC 
AACACGCCCT CCCCAAAGCC CTTGGAGGGG CGGCCAGAGC GGCAGTTCTT TGTGAAATGG 
CAAGGCATGT CTTACTGGCA CTGCTCCTGG GTTTCTGAAC TGCAGCTGGA GCTGCACTGT 
CAGGTGATGT TCCGAAACTA TCAGCGGAAG AATGATATGG ATGAGCCACC TTCTGGGGAC 
TTTGGTGGTG ATGAAGAGAA AAGCCGAAAG CGAAAGAACA AGGACCCTAA ATTTGCAGAG 
ATGGAGGAAC GCTTCTATCG CTATGGGATA AAACCCGAGT GGATGATGAT CCACCGAATC 
CTCAACCACA GTGTGGACAA GAAGGGCCAC GTCCACTACT TGATCAAGTG GCGGGACTTA 
CCTTACGATC AGGCTTCTTG GGAGAGTGAG GATGTGGAGA TCCAGGATTA CGACCTGTTC 
AAGCAGAGCT ATTGGAATCA CAGGGAGTTA ATGAGGGGTG AGGAAGGCCG ACCAGGCAAG 
AAGCTCAAGA AGGTGAAGCT TCGGAAGTTG GAGAGGCCTC CAGAAACGCC AACAGTTGAT 
CCAACAGTGA AGTATGAGCG ACAGCCAGAG TACCTGGATG CTACAGGTGG AACCCTGCAC 
CCCTATCAAA TGGAGGGCCT GAATTGGTTG CGCTTCTCCT GGGCTCAGGG CACTGACACC 
ATCTTGGCTG ATGAGATGGG CCTTGGGAAA ACTGTACAGA CAGCAGTCTT CCTGTATTCC 
CTTTACAAGG AGGGTCATTC CAAAGGCCCC TTCCTAGTGA GCGCCCCTCT TTCTACCATC 
ATCAACTGGG AGCGGGAGTT TGAAATGTGG GCTCCAGACA TGTATGTCGT AACCTATGTG 
GGTGACAAGG ACAGCCGTGC CATCATCCGA GAGAATGAGT TCTCCTTTGA AGACAATGCC 
ATTCGTGGTG GCAAGAAGGC CTCCCGCATG AAGAAAGAGG CATCTGTGAA ATTCCATGTG 
CTGCTGACAT CCTATGAATT GATCACCATT GACATGGCTA TTTTGGGCTC TATTGATTGG 
GCCTGCCTCA TCGTGGATGA AGCCCATCGG CTGAAGAACA ATCAGTCTAA GTTCTTCCGG 
GTATTGAATG GTTACTCACT CCAGCACAAG CTGTTGCTGA CTGGGACACC ATTACAAAAC 
AATCTGGAAG AGTTGTTTCA TCTGCTCAAC TTTCTCACCC CCGAGAGGTT CCACAATTTG 
GAAGGTTTTT TGGAGGAGTT TGCTGACATT GCCAAGGAGG ACCAGATAAA AAAACTGCAT 
GACATGCTGG GGCCGCACAT GTTGCGGCGG CTCAAAGCCG ATGTGTTCAA GAACATGCCC 
TCCAAGACAG AACTAATTGT GCGTGTGGAG CTGAGCCCTA TGCAGAAGAA ATACTACAAG 
TACATCCTCA CTCGAAATTT TGAAGCACTC AATGCCCGAG GTGGTGGCAA CCAGGTGTCT 
CTGCTGAATG TGGTGATGGA TCTTAAGAAG TGCTGCAACC ATCCATACCT CTTCCCTGTG 
GCTGCAATGG AAGCTCCTAA GATGCCTAAT GGCATGTATG ATGGCAGTGC CCTAATCAGA 
GCATCTGGGA AATTATTGCT GCTGCAGAAA ATGCTCAAGA ACCTTAAGGA GGGTGGGCAT 
CGTGTACTCA TCTTTTCCCA GATGACCAAG ATGCTAGACC TGCTAGAGGA TTTCTTGGAA 
CATGAAGGTT ATAAATACGA ACGCATCGAT GGTGGAATCA CTGGGAACAT GCGGCAAGAG 
GCCATTGACC GCTTCAATGC ACCGGGTGCT CAGCAGTTCT GCTTCTTGCT TTCCACTCGA 
GCTGGGGGCC TTGGAATCAA TCTGGCCACT GCTGACACAG TTATTATCTA TGACTCTGAC 
TGGAACCCCC ATAATGACAT TCAGGCCTTT AGCAGAGCTC ACCGGATTGG GCAAAATAAA 
AAGGTAATGA TCTACCGGTT TGTGACCCGT GCGTCAGTGG AGGAGCGCAT CACGCAGGTG 
GCAAAGAAGA AAATGATGCT GACGCATCTA GTGGTGCGGC CTGGGCTGGG CTCCAAGACT 
GGATCTATGT CCAAACAGGA GCTTGATGAT ATCCTCAAAT TTGGCACTGA GGAACTATTC 
AAGGATGAAG CCACTGATGG AGGAGGAGAC AACAAAGAGG GAGAAGATAG CAGTGTTATC 
CACTACGATG ATAAGGCCAT TGAACGGCTG CTAGACCGTA ACCAGGATGA GACTGAAGAC 
ACAGAATTGC AGGGCATGAA TGAATATTTG AGCTCATTCA AAGTGGCCCA GTATGTGGTA 
CGGGAAGAAG AAATGGGGGA GGAAGAGGAG GTAGAACGGG AAATCATTAA ACAGGAAGAA 
AGTGTGGATC CTGACTACTG GGAGAAATTG CTGCGGCACC ATTATGAGCA GCAGCAAGAA 
GATCTAGCCC GAAATCTGGG CAAAGGAAAA AGAATCCGTA AACAGGTCAA CTACAATGAT 
GGCTCCCAGG AGGACCGAGA TTGGCAGGAC GACCAGTCCG ACAACCAGTC CGATTACTCA 
GTGGCTTCAG AGGAAGGTGA TGAAGACTTT GATGAACGTT CAGAAGCTCC CCGTAGGCCC 
AGTCGTAAGG GCCTGCGGAA TGATAAAGAT AAGCCATTGC CTCCTCTGTT GGCCCGTGTT 
GGTGGGAATA TTGAAGTACT TGGTTTTAAT GCTCGTCAGC GAAAAGCCTT TCTTAATGCA 
ATTATGCGAT ATGGTATGCC ACCTCAGGAT GCTTTTACTA CCCAGTGGCT TGTAAGAGAC 
CTGCGAGGCA AATCAGAGAA AGAGTTCAAG GCATATGTCT CTCTTTTCAT GCGGCATTTA 
TGTGAGCCGG GGGCAGATGG GGCTGAGACC TTTGCTGATG GTGTCCCCCG AGAAGGCCTG 
TCTCGCCAGC ATGTCCTTAC TAGAATTGGT GTTATGTCTT TGATTCGCAA GAAGGTTCAG 
GAGTTTGAAC ATGTTAATGG GCGCTGGAGC ATGCCTGAAC TGGCTGAGGT GGAGGAAAAC 
AAGAAGATGT CCCAGCCAGG GTCACCCTCC CCAAAAACTC CTACACCCTC CACTCCAGGG 
GACACGCAGC CCAACACTCC TGCACCTGTC CCACCTGCTG AAGATGGGAT AAAAATAGAG 
GAAAATAGCC TCAAAGAAGA AGAGAGCATA GAAGGAGAAA AGGAGGTTAA ATCTACAGCC 
CCTGAGACTG CCATTGAGTG TACACAGGCC CCTGCCCCTG CCTCAGAGGA TGAAAAGGTC 
GTTGTTGAAC CCCCTGAGGG AGAGGAGAAA GTGGAAAAGG CAGAGGTGAA GGAGAGAACA 
GAGGAACCTA TGGAGACAGA GCCCAAAGGT GCTGCTGATG TAGAGAAGGT GGAGGAAAAG 
TCAGCAATAG ATCTGACCCC TATTGTGGTA GAAGACAAAG AAGAGAAGAA AGAAGAAGAA 
GAGAAAAAAG AGGTGATGCT TCAGAATGGA GAGACCCCCA AGGACCTGAA TGATGAGAAA 
CAGAAGAAAA ATATTAAACA ACGTTTCATG TTTAACATTG CAGATGGTGG TTTTACTGAG 
TTGCACTCCC TTTGGCAGAA TGAAGAGCGG GCAGCCACAG TTACCAAGAA GACTTATGAG 
ATCTGGCATC GACGGCATGA CTACTGGCTG CTAGCCGGCA TTATAAACCA TGGCTATGCC 
CGGTGGCAAG ACATCCAGAA TGACCCACGC TATGCCATCC TCAATGAGCC TTTCAAGGGT 
GAAATGAACC GTGGCAATTT CTTAGAGATC AAGAATAAAT TTCTAGCTCG AAGGTTTAAG 
CTCTTAGAAC AAGCTCTGGT GATTGAGGAA CAGCTGCGCC GGGCTGCTTA CTTGAACATG 
TCAGAAGACC CTTCTCACCC TTCCATGGCC CTCAACACCC GCTTTGCTGA GGTGGAGTGT 
TTGGCGGAAA GTCATCAGCA CCTGTCCAAG GAGTCAATGG CAGGAAACAA GCCAGCCAAT 
GCAGTCCTGC ACAAAGTTCT GAAACAGCTG GAAGAACTGC TGAGTGACAT GAAAGCTGAT 
GTGACTCGAC TCCCAGCTAC CATTGCCCGA ATTCCCCCAG TTGCTGTGAG GTTACAGATG 
TCAGAGCGTA ACATTCTCAG CCGCCTGGCA AACCGGGCAC CCGAACCTAC CCCACAGCAG 
GTAGCCCAGC AGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001284482.1
CDS	79..5796
Translation	MASGLGSPSPCSAGSEEEDMDALLNNSLPPPHPENEEDPEEDLSETETPKLKKKKKPKKPRDPKIPKSKRQKKELGDSSGEGPEFVEEEEEVALRSDSEGSDYTPGKKKKKKLGPKKEKKSKSKRKEEEEEEDDDDDSKEPKSSAQLLEDWGMEDIDHVFSEEDYRTLTNYKAFSQFVRPLIAAKNPKIAVSKMMMVLGAKWREFSTNNPFKGSSGASVAAAAAAAVAVVESMVTATEVAPPPPPVEVPIRKAKTKEGKGPNARRKPKGSPRVPDAKKPKPKKVAPLKIKLGGFGSKRKRSSSEDDDLDVESDFDDASINSYSVSDGSTSRSSRSRKKLRTTKKKKKGEEEVTAVDGYETDHQDYCEVCQQGGEIILCDTCPRAYHMVCLDPDMEKAPEGKWSCPHCEKEGIQWEAKEDNSEGEEILEEVGGDLEEEDDHHMEFCRVCKDGGELLCCDTCPSSYHIHCLNPPLPEIPNGEWLCPRCTCPALKGKVQKILIWKWGQPPSPTPVPRPPDADPNTPSPKPLEGRPERQFFVKWQGMSYWHCSWVSELQLELHCQVMFRNYQRKNDMDEPPSGDFGGDEEKSRKRKNKDPKFAEMEERFYRYGIKPEWMMIHRILNHSVDKKGHVHYLIKWRDLPYDQASWESEDVEIQDYDLFKQSYWNHRELMRGEEGRPGKKLKKVKLRKLERPPETPTVDPTVKYERQPEYLDATGGTLHPYQMEGLNWLRFSWAQGTDTILADEMGLGKTVQTAVFLYSLYKEGHSKGPFLVSAPLSTIINWEREFEMWAPDMYVVTYVGDKDSRAIIRENEFSFEDNAIRGGKKASRMKKEASVKFHVLLTSYELITIDMAILGSIDWACLIVDEAHRLKNNQSKFFRVLNGYSLQHKLLLTGTPLQNNLEELFHLLNFLTPERFHNLEGFLEEFADIAKEDQIKKLHDMLGPHMLRRLKADVFKNMPSKTELIVRVELSPMQKKYYKYILTRNFEALNARGGGNQVSLLNVVMDLKKCCNHPYLFPVAAMEAPKMPNGMYDGSALIRASGKLLLLQKMLKNLKEGGHRVLIFSQMTKMLDLLEDFLEHEGYKYERIDGGITGNMRQEAIDRFNAPGAQQFCFLLSTRAGGLGINLATADTVIIYDSDWNPHNDIQAFSRAHRIGQNKKVMIYRFVTRASVEERITQVAKKKMMLTHLVVRPGLGSKTGSMSKQELDDILKFGTEELFKDEATDGGGDNKEGEDSSVIHYDDKAIERLLDRNQDETEDTELQGMNEYLSSFKVAQYVVREEEMGEEEEVEREIIKQEESVDPDYWEKLLRHHYEQQQEDLARNLGKGKRIRKQVNYNDGSQEDRDWQDDQSDNQSDYSVASEEGDEDFDERSEAPRRPSRKGLRNDKDKPLPPLLARVGGNIEVLGFNARQRKAFLNAIMRYGMPPQDAFTTQWLVRDLRGKSEKEFKAYVSLFMRHLCEPGADGAETFADGVPREGLSRQHVLTRIGVMSLIRKKVQEFEHVNGRWSMPELAEVEENKKMSQPGSPSPKTPTPSTPGDTQPNTPAPVPPAEDGIKIEENSLKEEESIEGEKEVKSTAPETAIECTQAPAPASEDEKVVVEPPEGEEKVEKAEVKERTEEPMETEPKGAADVEKVEEKSAIDLTPIVVEDKEEKKEEEEKKEVMLQNGETPKDLNDEKQKKNIKQRFMFNIADGGFTELHSLWQNEERAATVTKKTYEIWHRRHDYWLLAGIINHGYARWQDIQNDPRYAILNEPFKGEMNRGNFLEIKNKFLARRFKLLEQALVIEEQLRRAAYLNMSEDPSHPSMALNTRFAEVECLAESHQHLSKESMAGNKPANAVLHKVLKQLEELLSDMKADVTRLPATIARIPPVAVRLQMSERNILSRLANRAPEPTPQQVAQQQ

Target ORF information:

RefSeq Version	NM_001297553.1
Organism	Homo sapiens(human)
Definition	Homo sapiens chromodomain helicase DNA binding protein 4 (CHD4), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001297553.1

ORF Insert Sequence:

ATGGCGTCGG GCCTGGGCTC CCCGTCCCCC TGCTCGGCGG GCAGTGAGGA GGAGGATATG 
GATGCACTTT TGAACAACAG CCTGCCCCCA CCCCACCCAG AAAATGAAGA GGACCCAGAA 
GAGGATTTGT CAGAAACAGA GACTCCAAAG CTCAAGAAGA AGAAAAAGCC TAAGAAACCT 
CGGGACCCTA AAATCCCTAA GAGCAAGCGC CAAAAAAAGG AGCTGGGGGA CAGCTCTGGG 
GAGGGGCCAG AGTTTGTGGA GGAGGAGGAA GAGGTGGCTC TGCGCTCAGA CAGTGAGGGC 
AGCGACTATA CTCCTGGCAA GAAGAAGAAG AAGAAGCTTG GACCTAAGAA AGAGAAGAAG 
AGCAAATCCA AGCGGAAGGA GGAGGAGGAG GAGGAGGATG ATGATGATGA TTCAAAGGAG 
CCTAAATCAT CTGCTCAGCT CCTGGAAGAC TGGGGCATGG AAGACATTGA CCACGTGTTC 
TCAGAGGAGG ATTATCGAAC CCTCACCAAC TACAAGGCCT TCAGCCAGTT TGTCAGACCC 
CTCATTGCTG CCAAAAATCC CAAGATTGCT GTCTCCAAGA TGATGATGGT TTTGGGTGCA 
AAATGGCGGG AGTTCAGTAC CAATAACCCC TTCAAAGGCA GTTCTGGGGC ATCAGTGGCA 
GCTGCGGCAG CAGCAGCGGT AGCTGTGGTG GAGAGCATGG TGACAGCCAC TGAGGTTGCA 
CCACCACCTC CCCCTGTGGA GGTGCCTATC CGCAAGGCCA AGACCAAGGA GGGCAAAGGT 
CCCAATGCTC GGAGGAAGCC CAAGGGCAGC CCTCGTGTAC CTGATGCCAA GAAGCCTAAA 
CCCAAGAAAG TAGCTCCCCT GAAAATCAAG CTGGGAGGTT TTGGTTCCAA GCGTAAGAGA 
TCCTCGAGTG AGGATGATGA CTTAGATGTG GAATCTGACT TCGATGATGC CAGTATCAAT 
AGCTATTCTG TTTCTGATGG TTCCACCAGC CGTAGTAGCC GCAGCCGCAA GAAACTCCGA 
ACCACTAAAA AGAAAAAGAA AGGCGAGGAG GAGGTGACTG CTGTGGATGG TTATGAGACA 
GACCACCAGG ACTATTGCGA GGTGTGCCAG CAAGGCGGTG AGATCATCCT GTGTGATACC 
TGTCCCCGTG CTTACCACAT GGTCTGCCTG GATCCCGACA TGGAGAAGGC TCCCGAGGGC 
AAGTGGAGCT GCCCACACTG CGAGAAGGAA GGCATCCAGT GGGAAGCTAA AGAGGACAAT 
TCGGAGGGTG AGGAGATCCT GGAAGAGGTT GGGGGAGACC TCGAAGAGGA GGATGACCAC 
CATATGGAAT TCTGTCGGGT CTGCAAGGAT GGTGGGGAAC TGCTCTGCTG TGATACCTGT 
CCTTCTTCCT ACCACATCCA CTGCCTGAAT CCCCCACTTC CAGAGATCCC CAACGGTGAA 
TGGCTCTGTC CCCGTTGTAC GTGTCCAGCT CTGAAGGGCA AAGTGCAGAA GATCCTAATC 
TGGAAGTGGG GTCAGCCACC ATCTCCCACA CCAGTGCCTC GGCCTCCAGA TGCTGATCCC 
AACACGCCCT CCCCAAAGCC CTTGGAGGGG CGGCCAGAGC GGCAGTTCTT TGTGAAATGG 
CAAGGCATGT CTTACTGGCA CTGCTCCTGG GTTTCTGAAC TGCAGCTGGA GCTGCACTGT 
CAGGTGATGT TCCGAAACTA TCAGCGGAAG AATGATATGG ATGAGCCACC TTCTGGGGAC 
TTTGGTGGTG ATGAAGAGAA AAGCCGAAAG CGAAAGAACA AGGACCCTAA ATTTGCAGAG 
ATGGAGGAAC GCTTCTATCG CTATGGGATA AAACCCGAGT GGATGATGAT CCACCGAATC 
CTCAACCACA GTGTGGACAA GAAGGGCCAC GTCCACTACT TGATCAAGTG GCGGGACTTA 
CCTTACGATC AGGCTTCTTG GGAGAGTGAG GATGTGGAGA TCCAGGATTA CGACCTGTTC 
AAGCAGAGCT ATTGGAATCA CAGGGAGTTA ATGAGGGGTG AGGAAGGCCG ACCAGGCAAG 
AAGCTCAAGA AGGTGAAGCT TCGGAAGTTG GAGAGGCCTC CAGAAACGCC AACAGTTGAT 
CCAACAGTGA AGTATGAGCG ACAGCCAGAG TACCTGGATG CTACAGGTGG AACCCTGCAC 
CCCTATCAAA TGGAGGGCCT GAATTGGTTG CGCTTCTCCT GGGCTCAGGG CACTGACACC 
ATCTTGGCTG ATGAGATGGG CCTTGGGAAA ACTGTACAGA CAGCAGTCTT CCTGTATTCC 
CTTTACAAGG AGGGTCATTC CAAAGGCCCC TTCCTAGTGA GCGCCCCTCT TTCTACCATC 
ATCAACTGGG AGCGGGAGTT TGAAATGTGG GCTCCAGACA TGTATGTCGT AACCTATGTG 
GGTGACAAGG ACAGCCGTGC CATCATCCGA GAGAATGAGT TCTCCTTTGA AGACAATGCC 
ATTCGTGGTG GCAAGAAGGC CTCCCGCATG AAGAAAGAGG CATCTGTGAA ATTCCATGTG 
CTGCTGACAT CCTATGAATT GATCACCATT GACATGGCTA TTTTGGGCTC TATTGATTGG 
GCCTGCCTCA TCGTGGATGA AGCCCATCGG CTGAAGAACA ATCAGTCTAA GTTCTTCCGG 
GTATTGAATG GTTACTCACT CCAGCACAAG CTGTTGCTGA CTGGGACACC ATTACAAAAC 
AATCTGGAAG AGTTGTTTCA TCTGCTCAAC TTTCTCACCC CCGAGAGGTT CCACAATTTG 
GAAGGTTTTT TGGAGGAGTT TGCTGACATT GCCAAGGAGG ACCAGATAAA AAAACTGCAT 
GACATGCTGG GGCCGCACAT GTTGCGGCGG CTCAAAGCCG ATGTGTTCAA GAACATGCCC 
TCCAAGACAG AACTAATTGT GCGTGTGGAG CTGAGCCCTA TGCAGAAGAA ATACTACAAG 
TACATCCTCA CTCGAAATTT TGAAGCACTC AATGCCCGAG GTGGTGGCAA CCAGGTGTCT 
CTGCTGAATG TGGTGATGGA TCTTAAGAAG TGCTGCAACC ATCCATACCT CTTCCCTGTG 
GCTGCAATGG AAGCTCCTAA GATGCCTAAT GGCATGTATG ATGGCAGTGC CCTAATCAGA 
GCATCTGGGA AATTATTGCT GCTGCAGAAA ATGCTCAAGA ACCTTAAGGA GGGTGGGCAT 
CGTGTACTCA TCTTTTCCCA GATGACCAAG ATGCTAGACC TGCTAGAGGA TTTCTTGGAA 
CATGAAGGTT ATAAATACGA ACGCATCGAT GGTGGAATCA CTGGGAACAT GCGGCAAGAG 
GCCATTGACC GCTTCAATGC ACCGGGTGCT CAGCAGTTCT GCTTCTTGCT TTCCACTCGA 
GCTGGGGGCC TTGGAATCAA TCTGGCCACT GCTGACACAG TTATTATCTA TGACTCTGAC 
TGGAACCCCC ATAATGACAT TCAGGCCTTT AGCAGAGCTC ACCGGATTGG GCAAAATAAA 
AAGGTAATGA TCTACCGGTT TGTGACCCGT GCGTCAGTGG AGGAGCGCAT CACGCAGGTG 
GCAAAGAAGA AAATGATGCT GACGCATCTA GTGGTGCGGC CTGGGCTGGG CTCCAAGACT 
GGATCTATGT CCAAACAGGA GCTTGATGAT ATCCTCAAAT TTGGCACTGA GGAACTATTC 
AAGGATGAAG CCACTGATGG AGGAGGAGAC AACAAAGAGG GAGAAGATAG CAGTGTTATC 
CACTACGATG ATAAGGCCAT TGAACGGCTG CTAGACCGTA ACCAGGATGA GACTGAAGAC 
ACAGAATTGC AGGGCATGAA TGAATATTTG AGCTCATTCA AAGTGGCCCA GTATGTGGTA 
CGGGAAGAAG AAATGGGGGA GGAAGAGGAG GTAGAACGGG AAATCATTAA ACAGGAAGAA 
AGTGTGGATC CTGACTACTG GGAGAAATTG CTGCGGCACC ATTATGAGCA GCAGCAAGAA 
GATCTAGCCC GAAATCTGGG CAAAGGAAAA AGAATCCGTA AACAGGTCAA CTACAATGAT 
GGCTCCCAGG AGGACCGAGA TTGGCAGGAC GACCAGTCCG ACAACCAGTC CGATTACTCA 
GTGGCTTCAG AGGAAGGTGA TGAAGACTTT GATGAACGTT CAGAAGCTCC CCGTAGGCCC 
AGTCGTAAGG GCCTGCGGAA TGATAAAGAT AAGCCATTGC CTCCTCTGTT GGCCCGTGTT 
GGTGGGAATA TTGAAGTACT TGGTTTTAAT GCTCGTCAGC GAAAAGCCTT TCTTAATGCA 
ATTATGCGAT ATGGTATGCC ACCTCAGGAT GCTTTTACTA CCCAGTGGCT TGTAAGAGAC 
CTGCGAGGCA AATCAGAGAA AGAGTTCAAG GCATATGTCT CTCTTTTCAT GCGGCATTTA 
TGTGAGCCGG GGGCAGATGG GGCTGAGACC TTTGCTGATG GTGTCCCCCG AGAAGGCCTG 
TCTCGCCAGC ATGTCCTTAC TAGAATTGGT GTTATGTCTT TGATTCGCAA GAAGGTTCAG 
GAGTTTGAAC ATGTTAATGG GCGCTGGAGC ATGCCTGAAC TGGCTGAGGT GGAGGAAAAC 
AAGAAGATGT CCCAGCCAGG GTCACCCTCC CCAAAAACTC CTACACCCTC CACTCCAGGG 
GACACGCAGC CCAACACTCC TGCACCTGTC CCACCTGCTG AAGATGGGAT AAAAATAGAG 
GAAAATAGCC TCAAAGAAGA AGAGAGCATA GAAGGAGAAA AGGAGGTTAA ATCTACAGCC 
CCTGAGACTG CCATTGAGTG TACACAGGCC CCTGCCCCTG CCTCAGAGGA TGAAAAGGTC 
GTTGTTGAAC CCCCTGAGGG AGAGGAGAAA GTGGAAAAGG CAGAGGTGAA GGAGAGAACA 
GAGGAACCTA TGGAGACAGA GCCCAAAGGT GCTGCTGATG TAGAGAAGGT GGAGGAAAAG 
TCAGCAATAG ATCTGACCCC TATTGTGGTA GAAGACAAAG AAGAGAAGAA AGAAGAAGAA 
GAGAAAAAAG AGGTGATGCT TCAGAATGGA GAGACCCCCA AGGACCTGAA TGATGAGAAA 
CAGAAGAAAA ATATTAAACA ACGTTTCATG TTTAACATTG CAGATGGTGG TTTTACTGAG 
TTGCACTCCC TTTGGCAGAA TGAAGAGCGG GCAGCCACAG TTACCAAGAA GACTTATGAG 
ATCTGGCATC GACGGCATGA CTACTGGCTG CTAGCCGGCA TTATAAACCA TGGCTATGCC 
CGGTGGCAAG ACATCCAGAA TGACCCACGC TATGCCATCC TCAATGAGCC TTTCAAGGGT 
GAAATGAACC GTGGCAATTT CTTAGAGATC AAGAATAAAT TTCTAGCTCG AAGGTTTAAG 
CTCTTAGAAC AAGCTCTGGT GATTGAGGAA CAGCTGCGCC GGGCTGCTTA CTTGAACATG 
TCAGAAGACC CTTCTCACCC TTCCATGGCC CTCAACACCC GCTTTGCTGA GGTGGAGTGT 
TTGGCGGAAA GTCATCAGCA CCTGTCCAAG GAGTCAATGG CAGGAAACAA GCCAGCCAAT 
GCAGTCCTGC ACAAAGTTCT GAAACAGCTG GAAGAACTGC TGAGTGACAT GAAAGCTGAT 
GTGACTCGAC TCCCAGCTAC CATTGCCCGA ATTCCCCCAG TTGCTGTGAG GTTACAGATG 
TCAGAGCGTA ACATTCTCAG CCGCCTGGCA AACCGGGCAC CCGAACCTAC CCCACAGCAG 
GTAGCCCAGC AGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHD4 ( NM_001297553.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001284482.1 Homo sapiens chromodomain helicase DNA binding protein 4 (CHD4), transcript variant 2, mRNA.

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CloneID	OHu28781
Clone ID Related Accession (Same CDS sequence)	NM_001297553.1 , NM_001297553.2 , XM_005253668.3
Accession Version	NM_001297553.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5718bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-06
Organism	Homo sapiens(human)
Product	chromodomain-helicase-DNA-binding protein 4 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from X86691.1, CR936680.1, AC006064.10 and BC038596.2. On May 24, 2018 this sequence version replaced NM_001297553.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CR936680.1, SRR1803613.266802.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGGCGTCGG GCCTGGGCTC CCCGTCCCCC TGCTCGGCGG GCAGTGAGGA GGAGGATATG 
GATGCACTTT TGAACAACAG CCTGCCCCCA CCCCACCCAG AAAATGAAGA GGACCCAGAA 
GAGGATTTGT CAGAAACAGA GACTCCAAAG CTCAAGAAGA AGAAAAAGCC TAAGAAACCT 
CGGGACCCTA AAATCCCTAA GAGCAAGCGC CAAAAAAAGG AGCTGGGGGA CAGCTCTGGG 
GAGGGGCCAG AGTTTGTGGA GGAGGAGGAA GAGGTGGCTC TGCGCTCAGA CAGTGAGGGC 
AGCGACTATA CTCCTGGCAA GAAGAAGAAG AAGAAGCTTG GACCTAAGAA AGAGAAGAAG 
AGCAAATCCA AGCGGAAGGA GGAGGAGGAG GAGGAGGATG ATGATGATGA TTCAAAGGAG 
CCTAAATCAT CTGCTCAGCT CCTGGAAGAC TGGGGCATGG AAGACATTGA CCACGTGTTC 
TCAGAGGAGG ATTATCGAAC CCTCACCAAC TACAAGGCCT TCAGCCAGTT TGTCAGACCC 
CTCATTGCTG CCAAAAATCC CAAGATTGCT GTCTCCAAGA TGATGATGGT TTTGGGTGCA 
AAATGGCGGG AGTTCAGTAC CAATAACCCC TTCAAAGGCA GTTCTGGGGC ATCAGTGGCA 
GCTGCGGCAG CAGCAGCGGT AGCTGTGGTG GAGAGCATGG TGACAGCCAC TGAGGTTGCA 
CCACCACCTC CCCCTGTGGA GGTGCCTATC CGCAAGGCCA AGACCAAGGA GGGCAAAGGT 
CCCAATGCTC GGAGGAAGCC CAAGGGCAGC CCTCGTGTAC CTGATGCCAA GAAGCCTAAA 
CCCAAGAAAG TAGCTCCCCT GAAAATCAAG CTGGGAGGTT TTGGTTCCAA GCGTAAGAGA 
TCCTCGAGTG AGGATGATGA CTTAGATGTG GAATCTGACT TCGATGATGC CAGTATCAAT 
AGCTATTCTG TTTCTGATGG TTCCACCAGC CGTAGTAGCC GCAGCCGCAA GAAACTCCGA 
ACCACTAAAA AGAAAAAGAA AGGCGAGGAG GAGGTGACTG CTGTGGATGG TTATGAGACA 
GACCACCAGG ACTATTGCGA GGTGTGCCAG CAAGGCGGTG AGATCATCCT GTGTGATACC 
TGTCCCCGTG CTTACCACAT GGTCTGCCTG GATCCCGACA TGGAGAAGGC TCCCGAGGGC 
AAGTGGAGCT GCCCACACTG CGAGAAGGAA GGCATCCAGT GGGAAGCTAA AGAGGACAAT 
TCGGAGGGTG AGGAGATCCT GGAAGAGGTT GGGGGAGACC TCGAAGAGGA GGATGACCAC 
CATATGGAAT TCTGTCGGGT CTGCAAGGAT GGTGGGGAAC TGCTCTGCTG TGATACCTGT 
CCTTCTTCCT ACCACATCCA CTGCCTGAAT CCCCCACTTC CAGAGATCCC CAACGGTGAA 
TGGCTCTGTC CCCGTTGTAC GTGTCCAGCT CTGAAGGGCA AAGTGCAGAA GATCCTAATC 
TGGAAGTGGG GTCAGCCACC ATCTCCCACA CCAGTGCCTC GGCCTCCAGA TGCTGATCCC 
AACACGCCCT CCCCAAAGCC CTTGGAGGGG CGGCCAGAGC GGCAGTTCTT TGTGAAATGG 
CAAGGCATGT CTTACTGGCA CTGCTCCTGG GTTTCTGAAC TGCAGCTGGA GCTGCACTGT 
CAGGTGATGT TCCGAAACTA TCAGCGGAAG AATGATATGG ATGAGCCACC TTCTGGGGAC 
TTTGGTGGTG ATGAAGAGAA AAGCCGAAAG CGAAAGAACA AGGACCCTAA ATTTGCAGAG 
ATGGAGGAAC GCTTCTATCG CTATGGGATA AAACCCGAGT GGATGATGAT CCACCGAATC 
CTCAACCACA GTGTGGACAA GAAGGGCCAC GTCCACTACT TGATCAAGTG GCGGGACTTA 
CCTTACGATC AGGCTTCTTG GGAGAGTGAG GATGTGGAGA TCCAGGATTA CGACCTGTTC 
AAGCAGAGCT ATTGGAATCA CAGGGAGTTA ATGAGGGGTG AGGAAGGCCG ACCAGGCAAG 
AAGCTCAAGA AGGTGAAGCT TCGGAAGTTG GAGAGGCCTC CAGAAACGCC AACAGTTGAT 
CCAACAGTGA AGTATGAGCG ACAGCCAGAG TACCTGGATG CTACAGGTGG AACCCTGCAC 
CCCTATCAAA TGGAGGGCCT GAATTGGTTG CGCTTCTCCT GGGCTCAGGG CACTGACACC 
ATCTTGGCTG ATGAGATGGG CCTTGGGAAA ACTGTACAGA CAGCAGTCTT CCTGTATTCC 
CTTTACAAGG AGGGTCATTC CAAAGGCCCC TTCCTAGTGA GCGCCCCTCT TTCTACCATC 
ATCAACTGGG AGCGGGAGTT TGAAATGTGG GCTCCAGACA TGTATGTCGT AACCTATGTG 
GGTGACAAGG ACAGCCGTGC CATCATCCGA GAGAATGAGT TCTCCTTTGA AGACAATGCC 
ATTCGTGGTG GCAAGAAGGC CTCCCGCATG AAGAAAGAGG CATCTGTGAA ATTCCATGTG 
CTGCTGACAT CCTATGAATT GATCACCATT GACATGGCTA TTTTGGGCTC TATTGATTGG 
GCCTGCCTCA TCGTGGATGA AGCCCATCGG CTGAAGAACA ATCAGTCTAA GTTCTTCCGG 
GTATTGAATG GTTACTCACT CCAGCACAAG CTGTTGCTGA CTGGGACACC ATTACAAAAC 
AATCTGGAAG AGTTGTTTCA TCTGCTCAAC TTTCTCACCC CCGAGAGGTT CCACAATTTG 
GAAGGTTTTT TGGAGGAGTT TGCTGACATT GCCAAGGAGG ACCAGATAAA AAAACTGCAT 
GACATGCTGG GGCCGCACAT GTTGCGGCGG CTCAAAGCCG ATGTGTTCAA GAACATGCCC 
TCCAAGACAG AACTAATTGT GCGTGTGGAG CTGAGCCCTA TGCAGAAGAA ATACTACAAG 
TACATCCTCA CTCGAAATTT TGAAGCACTC AATGCCCGAG GTGGTGGCAA CCAGGTGTCT 
CTGCTGAATG TGGTGATGGA TCTTAAGAAG TGCTGCAACC ATCCATACCT CTTCCCTGTG 
GCTGCAATGG AAGCTCCTAA GATGCCTAAT GGCATGTATG ATGGCAGTGC CCTAATCAGA 
GCATCTGGGA AATTATTGCT GCTGCAGAAA ATGCTCAAGA ACCTTAAGGA GGGTGGGCAT 
CGTGTACTCA TCTTTTCCCA GATGACCAAG ATGCTAGACC TGCTAGAGGA TTTCTTGGAA 
CATGAAGGTT ATAAATACGA ACGCATCGAT GGTGGAATCA CTGGGAACAT GCGGCAAGAG 
GCCATTGACC GCTTCAATGC ACCGGGTGCT CAGCAGTTCT GCTTCTTGCT TTCCACTCGA 
GCTGGGGGCC TTGGAATCAA TCTGGCCACT GCTGACACAG TTATTATCTA TGACTCTGAC 
TGGAACCCCC ATAATGACAT TCAGGCCTTT AGCAGAGCTC ACCGGATTGG GCAAAATAAA 
AAGGTAATGA TCTACCGGTT TGTGACCCGT GCGTCAGTGG AGGAGCGCAT CACGCAGGTG 
GCAAAGAAGA AAATGATGCT GACGCATCTA GTGGTGCGGC CTGGGCTGGG CTCCAAGACT 
GGATCTATGT CCAAACAGGA GCTTGATGAT ATCCTCAAAT TTGGCACTGA GGAACTATTC 
AAGGATGAAG CCACTGATGG AGGAGGAGAC AACAAAGAGG GAGAAGATAG CAGTGTTATC 
CACTACGATG ATAAGGCCAT TGAACGGCTG CTAGACCGTA ACCAGGATGA GACTGAAGAC 
ACAGAATTGC AGGGCATGAA TGAATATTTG AGCTCATTCA AAGTGGCCCA GTATGTGGTA 
CGGGAAGAAG AAATGGGGGA GGAAGAGGAG GTAGAACGGG AAATCATTAA ACAGGAAGAA 
AGTGTGGATC CTGACTACTG GGAGAAATTG CTGCGGCACC ATTATGAGCA GCAGCAAGAA 
GATCTAGCCC GAAATCTGGG CAAAGGAAAA AGAATCCGTA AACAGGTCAA CTACAATGAT 
GGCTCCCAGG AGGACCGAGA TTGGCAGGAC GACCAGTCCG ACAACCAGTC CGATTACTCA 
GTGGCTTCAG AGGAAGGTGA TGAAGACTTT GATGAACGTT CAGAAGCTCC CCGTAGGCCC 
AGTCGTAAGG GCCTGCGGAA TGATAAAGAT AAGCCATTGC CTCCTCTGTT GGCCCGTGTT 
GGTGGGAATA TTGAAGTACT TGGTTTTAAT GCTCGTCAGC GAAAAGCCTT TCTTAATGCA 
ATTATGCGAT ATGGTATGCC ACCTCAGGAT GCTTTTACTA CCCAGTGGCT TGTAAGAGAC 
CTGCGAGGCA AATCAGAGAA AGAGTTCAAG GCATATGTCT CTCTTTTCAT GCGGCATTTA 
TGTGAGCCGG GGGCAGATGG GGCTGAGACC TTTGCTGATG GTGTCCCCCG AGAAGGCCTG 
TCTCGCCAGC ATGTCCTTAC TAGAATTGGT GTTATGTCTT TGATTCGCAA GAAGGTTCAG 
GAGTTTGAAC ATGTTAATGG GCGCTGGAGC ATGCCTGAAC TGGCTGAGGT GGAGGAAAAC 
AAGAAGATGT CCCAGCCAGG GTCACCCTCC CCAAAAACTC CTACACCCTC CACTCCAGGG 
GACACGCAGC CCAACACTCC TGCACCTGTC CCACCTGCTG AAGATGGGAT AAAAATAGAG 
GAAAATAGCC TCAAAGAAGA AGAGAGCATA GAAGGAGAAA AGGAGGTTAA ATCTACAGCC 
CCTGAGACTG CCATTGAGTG TACACAGGCC CCTGCCCCTG CCTCAGAGGA TGAAAAGGTC 
GTTGTTGAAC CCCCTGAGGG AGAGGAGAAA GTGGAAAAGG CAGAGGTGAA GGAGAGAACA 
GAGGAACCTA TGGAGACAGA GCCCAAAGGT GCTGCTGATG TAGAGAAGGT GGAGGAAAAG 
TCAGCAATAG ATCTGACCCC TATTGTGGTA GAAGACAAAG AAGAGAAGAA AGAAGAAGAA 
GAGAAAAAAG AGGTGATGCT TCAGAATGGA GAGACCCCCA AGGACCTGAA TGATGAGAAA 
CAGAAGAAAA ATATTAAACA ACGTTTCATG TTTAACATTG CAGATGGTGG TTTTACTGAG 
TTGCACTCCC TTTGGCAGAA TGAAGAGCGG GCAGCCACAG TTACCAAGAA GACTTATGAG 
ATCTGGCATC GACGGCATGA CTACTGGCTG CTAGCCGGCA TTATAAACCA TGGCTATGCC 
CGGTGGCAAG ACATCCAGAA TGACCCACGC TATGCCATCC TCAATGAGCC TTTCAAGGGT 
GAAATGAACC GTGGCAATTT CTTAGAGATC AAGAATAAAT TTCTAGCTCG AAGGTTTAAG 
CTCTTAGAAC AAGCTCTGGT GATTGAGGAA CAGCTGCGCC GGGCTGCTTA CTTGAACATG 
TCAGAAGACC CTTCTCACCC TTCCATGGCC CTCAACACCC GCTTTGCTGA GGTGGAGTGT 
TTGGCGGAAA GTCATCAGCA CCTGTCCAAG GAGTCAATGG CAGGAAACAA GCCAGCCAAT 
GCAGTCCTGC ACAAAGTTCT GAAACAGCTG GAAGAACTGC TGAGTGACAT GAAAGCTGAT 
GTGACTCGAC TCCCAGCTAC CATTGCCCGA ATTCCCCCAG TTGCTGTGAG GTTACAGATG 
TCAGAGCGTA ACATTCTCAG CCGCCTGGCA AACCGGGCAC CCGAACCTAC CCCACAGCAG 
GTAGCCCAGC AGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001284482.1
CDS	79..5796
Translation	MASGLGSPSPCSAGSEEEDMDALLNNSLPPPHPENEEDPEEDLSETETPKLKKKKKPKKPRDPKIPKSKRQKKELGDSSGEGPEFVEEEEEVALRSDSEGSDYTPGKKKKKKLGPKKEKKSKSKRKEEEEEEDDDDDSKEPKSSAQLLEDWGMEDIDHVFSEEDYRTLTNYKAFSQFVRPLIAAKNPKIAVSKMMMVLGAKWREFSTNNPFKGSSGASVAAAAAAAVAVVESMVTATEVAPPPPPVEVPIRKAKTKEGKGPNARRKPKGSPRVPDAKKPKPKKVAPLKIKLGGFGSKRKRSSSEDDDLDVESDFDDASINSYSVSDGSTSRSSRSRKKLRTTKKKKKGEEEVTAVDGYETDHQDYCEVCQQGGEIILCDTCPRAYHMVCLDPDMEKAPEGKWSCPHCEKEGIQWEAKEDNSEGEEILEEVGGDLEEEDDHHMEFCRVCKDGGELLCCDTCPSSYHIHCLNPPLPEIPNGEWLCPRCTCPALKGKVQKILIWKWGQPPSPTPVPRPPDADPNTPSPKPLEGRPERQFFVKWQGMSYWHCSWVSELQLELHCQVMFRNYQRKNDMDEPPSGDFGGDEEKSRKRKNKDPKFAEMEERFYRYGIKPEWMMIHRILNHSVDKKGHVHYLIKWRDLPYDQASWESEDVEIQDYDLFKQSYWNHRELMRGEEGRPGKKLKKVKLRKLERPPETPTVDPTVKYERQPEYLDATGGTLHPYQMEGLNWLRFSWAQGTDTILADEMGLGKTVQTAVFLYSLYKEGHSKGPFLVSAPLSTIINWEREFEMWAPDMYVVTYVGDKDSRAIIRENEFSFEDNAIRGGKKASRMKKEASVKFHVLLTSYELITIDMAILGSIDWACLIVDEAHRLKNNQSKFFRVLNGYSLQHKLLLTGTPLQNNLEELFHLLNFLTPERFHNLEGFLEEFADIAKEDQIKKLHDMLGPHMLRRLKADVFKNMPSKTELIVRVELSPMQKKYYKYILTRNFEALNARGGGNQVSLLNVVMDLKKCCNHPYLFPVAAMEAPKMPNGMYDGSALIRASGKLLLLQKMLKNLKEGGHRVLIFSQMTKMLDLLEDFLEHEGYKYERIDGGITGNMRQEAIDRFNAPGAQQFCFLLSTRAGGLGINLATADTVIIYDSDWNPHNDIQAFSRAHRIGQNKKVMIYRFVTRASVEERITQVAKKKMMLTHLVVRPGLGSKTGSMSKQELDDILKFGTEELFKDEATDGGGDNKEGEDSSVIHYDDKAIERLLDRNQDETEDTELQGMNEYLSSFKVAQYVVREEEMGEEEEVEREIIKQEESVDPDYWEKLLRHHYEQQQEDLARNLGKGKRIRKQVNYNDGSQEDRDWQDDQSDNQSDYSVASEEGDEDFDERSEAPRRPSRKGLRNDKDKPLPPLLARVGGNIEVLGFNARQRKAFLNAIMRYGMPPQDAFTTQWLVRDLRGKSEKEFKAYVSLFMRHLCEPGADGAETFADGVPREGLSRQHVLTRIGVMSLIRKKVQEFEHVNGRWSMPELAEVEENKKMSQPGSPSPKTPTPSTPGDTQPNTPAPVPPAEDGIKIEENSLKEEESIEGEKEVKSTAPETAIECTQAPAPASEDEKVVVEPPEGEEKVEKAEVKERTEEPMETEPKGAADVEKVEEKSAIDLTPIVVEDKEEKKEEEEKKEVMLQNGETPKDLNDEKQKKNIKQRFMFNIADGGFTELHSLWQNEERAATVTKKTYEIWHRRHDYWLLAGIINHGYARWQDIQNDPRYAILNEPFKGEMNRGNFLEIKNKFLARRFKLLEQALVIEEQLRRAAYLNMSEDPSHPSMALNTRFAEVECLAESHQHLSKESMAGNKPANAVLHKVLKQLEELLSDMKADVTRLPATIARIPPVAVRLQMSERNILSRLANRAPEPTPQQVAQQQ

Target ORF information:

RefSeq Version	NM_001297553.2
Organism	Homo sapiens(human)
Definition	Homo sapiens chromodomain helicase DNA binding protein 4 (CHD4), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001297553.2

ORF Insert Sequence:

ATGGCGTCGG GCCTGGGCTC CCCGTCCCCC TGCTCGGCGG GCAGTGAGGA GGAGGATATG 
GATGCACTTT TGAACAACAG CCTGCCCCCA CCCCACCCAG AAAATGAAGA GGACCCAGAA 
GAGGATTTGT CAGAAACAGA GACTCCAAAG CTCAAGAAGA AGAAAAAGCC TAAGAAACCT 
CGGGACCCTA AAATCCCTAA GAGCAAGCGC CAAAAAAAGG AGCTGGGGGA CAGCTCTGGG 
GAGGGGCCAG AGTTTGTGGA GGAGGAGGAA GAGGTGGCTC TGCGCTCAGA CAGTGAGGGC 
AGCGACTATA CTCCTGGCAA GAAGAAGAAG AAGAAGCTTG GACCTAAGAA AGAGAAGAAG 
AGCAAATCCA AGCGGAAGGA GGAGGAGGAG GAGGAGGATG ATGATGATGA TTCAAAGGAG 
CCTAAATCAT CTGCTCAGCT CCTGGAAGAC TGGGGCATGG AAGACATTGA CCACGTGTTC 
TCAGAGGAGG ATTATCGAAC CCTCACCAAC TACAAGGCCT TCAGCCAGTT TGTCAGACCC 
CTCATTGCTG CCAAAAATCC CAAGATTGCT GTCTCCAAGA TGATGATGGT TTTGGGTGCA 
AAATGGCGGG AGTTCAGTAC CAATAACCCC TTCAAAGGCA GTTCTGGGGC ATCAGTGGCA 
GCTGCGGCAG CAGCAGCGGT AGCTGTGGTG GAGAGCATGG TGACAGCCAC TGAGGTTGCA 
CCACCACCTC CCCCTGTGGA GGTGCCTATC CGCAAGGCCA AGACCAAGGA GGGCAAAGGT 
CCCAATGCTC GGAGGAAGCC CAAGGGCAGC CCTCGTGTAC CTGATGCCAA GAAGCCTAAA 
CCCAAGAAAG TAGCTCCCCT GAAAATCAAG CTGGGAGGTT TTGGTTCCAA GCGTAAGAGA 
TCCTCGAGTG AGGATGATGA CTTAGATGTG GAATCTGACT TCGATGATGC CAGTATCAAT 
AGCTATTCTG TTTCTGATGG TTCCACCAGC CGTAGTAGCC GCAGCCGCAA GAAACTCCGA 
ACCACTAAAA AGAAAAAGAA AGGCGAGGAG GAGGTGACTG CTGTGGATGG TTATGAGACA 
GACCACCAGG ACTATTGCGA GGTGTGCCAG CAAGGCGGTG AGATCATCCT GTGTGATACC 
TGTCCCCGTG CTTACCACAT GGTCTGCCTG GATCCCGACA TGGAGAAGGC TCCCGAGGGC 
AAGTGGAGCT GCCCACACTG CGAGAAGGAA GGCATCCAGT GGGAAGCTAA AGAGGACAAT 
TCGGAGGGTG AGGAGATCCT GGAAGAGGTT GGGGGAGACC TCGAAGAGGA GGATGACCAC 
CATATGGAAT TCTGTCGGGT CTGCAAGGAT GGTGGGGAAC TGCTCTGCTG TGATACCTGT 
CCTTCTTCCT ACCACATCCA CTGCCTGAAT CCCCCACTTC CAGAGATCCC CAACGGTGAA 
TGGCTCTGTC CCCGTTGTAC GTGTCCAGCT CTGAAGGGCA AAGTGCAGAA GATCCTAATC 
TGGAAGTGGG GTCAGCCACC ATCTCCCACA CCAGTGCCTC GGCCTCCAGA TGCTGATCCC 
AACACGCCCT CCCCAAAGCC CTTGGAGGGG CGGCCAGAGC GGCAGTTCTT TGTGAAATGG 
CAAGGCATGT CTTACTGGCA CTGCTCCTGG GTTTCTGAAC TGCAGCTGGA GCTGCACTGT 
CAGGTGATGT TCCGAAACTA TCAGCGGAAG AATGATATGG ATGAGCCACC TTCTGGGGAC 
TTTGGTGGTG ATGAAGAGAA AAGCCGAAAG CGAAAGAACA AGGACCCTAA ATTTGCAGAG 
ATGGAGGAAC GCTTCTATCG CTATGGGATA AAACCCGAGT GGATGATGAT CCACCGAATC 
CTCAACCACA GTGTGGACAA GAAGGGCCAC GTCCACTACT TGATCAAGTG GCGGGACTTA 
CCTTACGATC AGGCTTCTTG GGAGAGTGAG GATGTGGAGA TCCAGGATTA CGACCTGTTC 
AAGCAGAGCT ATTGGAATCA CAGGGAGTTA ATGAGGGGTG AGGAAGGCCG ACCAGGCAAG 
AAGCTCAAGA AGGTGAAGCT TCGGAAGTTG GAGAGGCCTC CAGAAACGCC AACAGTTGAT 
CCAACAGTGA AGTATGAGCG ACAGCCAGAG TACCTGGATG CTACAGGTGG AACCCTGCAC 
CCCTATCAAA TGGAGGGCCT GAATTGGTTG CGCTTCTCCT GGGCTCAGGG CACTGACACC 
ATCTTGGCTG ATGAGATGGG CCTTGGGAAA ACTGTACAGA CAGCAGTCTT CCTGTATTCC 
CTTTACAAGG AGGGTCATTC CAAAGGCCCC TTCCTAGTGA GCGCCCCTCT TTCTACCATC 
ATCAACTGGG AGCGGGAGTT TGAAATGTGG GCTCCAGACA TGTATGTCGT AACCTATGTG 
GGTGACAAGG ACAGCCGTGC CATCATCCGA GAGAATGAGT TCTCCTTTGA AGACAATGCC 
ATTCGTGGTG GCAAGAAGGC CTCCCGCATG AAGAAAGAGG CATCTGTGAA ATTCCATGTG 
CTGCTGACAT CCTATGAATT GATCACCATT GACATGGCTA TTTTGGGCTC TATTGATTGG 
GCCTGCCTCA TCGTGGATGA AGCCCATCGG CTGAAGAACA ATCAGTCTAA GTTCTTCCGG 
GTATTGAATG GTTACTCACT CCAGCACAAG CTGTTGCTGA CTGGGACACC ATTACAAAAC 
AATCTGGAAG AGTTGTTTCA TCTGCTCAAC TTTCTCACCC CCGAGAGGTT CCACAATTTG 
GAAGGTTTTT TGGAGGAGTT TGCTGACATT GCCAAGGAGG ACCAGATAAA AAAACTGCAT 
GACATGCTGG GGCCGCACAT GTTGCGGCGG CTCAAAGCCG ATGTGTTCAA GAACATGCCC 
TCCAAGACAG AACTAATTGT GCGTGTGGAG CTGAGCCCTA TGCAGAAGAA ATACTACAAG 
TACATCCTCA CTCGAAATTT TGAAGCACTC AATGCCCGAG GTGGTGGCAA CCAGGTGTCT 
CTGCTGAATG TGGTGATGGA TCTTAAGAAG TGCTGCAACC ATCCATACCT CTTCCCTGTG 
GCTGCAATGG AAGCTCCTAA GATGCCTAAT GGCATGTATG ATGGCAGTGC CCTAATCAGA 
GCATCTGGGA AATTATTGCT GCTGCAGAAA ATGCTCAAGA ACCTTAAGGA GGGTGGGCAT 
CGTGTACTCA TCTTTTCCCA GATGACCAAG ATGCTAGACC TGCTAGAGGA TTTCTTGGAA 
CATGAAGGTT ATAAATACGA ACGCATCGAT GGTGGAATCA CTGGGAACAT GCGGCAAGAG 
GCCATTGACC GCTTCAATGC ACCGGGTGCT CAGCAGTTCT GCTTCTTGCT TTCCACTCGA 
GCTGGGGGCC TTGGAATCAA TCTGGCCACT GCTGACACAG TTATTATCTA TGACTCTGAC 
TGGAACCCCC ATAATGACAT TCAGGCCTTT AGCAGAGCTC ACCGGATTGG GCAAAATAAA 
AAGGTAATGA TCTACCGGTT TGTGACCCGT GCGTCAGTGG AGGAGCGCAT CACGCAGGTG 
GCAAAGAAGA AAATGATGCT GACGCATCTA GTGGTGCGGC CTGGGCTGGG CTCCAAGACT 
GGATCTATGT CCAAACAGGA GCTTGATGAT ATCCTCAAAT TTGGCACTGA GGAACTATTC 
AAGGATGAAG CCACTGATGG AGGAGGAGAC AACAAAGAGG GAGAAGATAG CAGTGTTATC 
CACTACGATG ATAAGGCCAT TGAACGGCTG CTAGACCGTA ACCAGGATGA GACTGAAGAC 
ACAGAATTGC AGGGCATGAA TGAATATTTG AGCTCATTCA AAGTGGCCCA GTATGTGGTA 
CGGGAAGAAG AAATGGGGGA GGAAGAGGAG GTAGAACGGG AAATCATTAA ACAGGAAGAA 
AGTGTGGATC CTGACTACTG GGAGAAATTG CTGCGGCACC ATTATGAGCA GCAGCAAGAA 
GATCTAGCCC GAAATCTGGG CAAAGGAAAA AGAATCCGTA AACAGGTCAA CTACAATGAT 
GGCTCCCAGG AGGACCGAGA TTGGCAGGAC GACCAGTCCG ACAACCAGTC CGATTACTCA 
GTGGCTTCAG AGGAAGGTGA TGAAGACTTT GATGAACGTT CAGAAGCTCC CCGTAGGCCC 
AGTCGTAAGG GCCTGCGGAA TGATAAAGAT AAGCCATTGC CTCCTCTGTT GGCCCGTGTT 
GGTGGGAATA TTGAAGTACT TGGTTTTAAT GCTCGTCAGC GAAAAGCCTT TCTTAATGCA 
ATTATGCGAT ATGGTATGCC ACCTCAGGAT GCTTTTACTA CCCAGTGGCT TGTAAGAGAC 
CTGCGAGGCA AATCAGAGAA AGAGTTCAAG GCATATGTCT CTCTTTTCAT GCGGCATTTA 
TGTGAGCCGG GGGCAGATGG GGCTGAGACC TTTGCTGATG GTGTCCCCCG AGAAGGCCTG 
TCTCGCCAGC ATGTCCTTAC TAGAATTGGT GTTATGTCTT TGATTCGCAA GAAGGTTCAG 
GAGTTTGAAC ATGTTAATGG GCGCTGGAGC ATGCCTGAAC TGGCTGAGGT GGAGGAAAAC 
AAGAAGATGT CCCAGCCAGG GTCACCCTCC CCAAAAACTC CTACACCCTC CACTCCAGGG 
GACACGCAGC CCAACACTCC TGCACCTGTC CCACCTGCTG AAGATGGGAT AAAAATAGAG 
GAAAATAGCC TCAAAGAAGA AGAGAGCATA GAAGGAGAAA AGGAGGTTAA ATCTACAGCC 
CCTGAGACTG CCATTGAGTG TACACAGGCC CCTGCCCCTG CCTCAGAGGA TGAAAAGGTC 
GTTGTTGAAC CCCCTGAGGG AGAGGAGAAA GTGGAAAAGG CAGAGGTGAA GGAGAGAACA 
GAGGAACCTA TGGAGACAGA GCCCAAAGGT GCTGCTGATG TAGAGAAGGT GGAGGAAAAG 
TCAGCAATAG ATCTGACCCC TATTGTGGTA GAAGACAAAG AAGAGAAGAA AGAAGAAGAA 
GAGAAAAAAG AGGTGATGCT TCAGAATGGA GAGACCCCCA AGGACCTGAA TGATGAGAAA 
CAGAAGAAAA ATATTAAACA ACGTTTCATG TTTAACATTG CAGATGGTGG TTTTACTGAG 
TTGCACTCCC TTTGGCAGAA TGAAGAGCGG GCAGCCACAG TTACCAAGAA GACTTATGAG 
ATCTGGCATC GACGGCATGA CTACTGGCTG CTAGCCGGCA TTATAAACCA TGGCTATGCC 
CGGTGGCAAG ACATCCAGAA TGACCCACGC TATGCCATCC TCAATGAGCC TTTCAAGGGT 
GAAATGAACC GTGGCAATTT CTTAGAGATC AAGAATAAAT TTCTAGCTCG AAGGTTTAAG 
CTCTTAGAAC AAGCTCTGGT GATTGAGGAA CAGCTGCGCC GGGCTGCTTA CTTGAACATG 
TCAGAAGACC CTTCTCACCC TTCCATGGCC CTCAACACCC GCTTTGCTGA GGTGGAGTGT 
TTGGCGGAAA GTCATCAGCA CCTGTCCAAG GAGTCAATGG CAGGAAACAA GCCAGCCAAT 
GCAGTCCTGC ACAAAGTTCT GAAACAGCTG GAAGAACTGC TGAGTGACAT GAAAGCTGAT 
GTGACTCGAC TCCCAGCTAC CATTGCCCGA ATTCCCCCAG TTGCTGTGAG GTTACAGATG 
TCAGAGCGTA ACATTCTCAG CCGCCTGGCA AACCGGGCAC CCGAACCTAC CCCACAGCAG 
GTAGCCCAGC AGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHD4 ( NM_001363606.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001350535.1 Homo sapiens chromodomain helicase DNA binding protein 4 (CHD4), transcript variant 3, mRNA.

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CloneID	OHu45954
Clone ID Related Accession (Same CDS sequence)	XM_006718962.1 , NM_001363606.1 , NM_001363606.2
Accession Version	NM_001363606.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5709bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-27
Organism	Homo sapiens(human)
Product	chromodomain-helicase-DNA-binding protein 4 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC006064.10. On May 24, 2018 this sequence version replaced XM_006718962.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.258631.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGGCGTCGG GCCTGGGCTC CCCGTCCCCC TGCTCGGCGG GCAGTGAGGA GGAGGATATG 
GATGCACTTT TGAACAACAG CCTGCCCCCA CCCCACCCAG AAAATGAAGA GGACCCAGAA 
GAGGATTTGT CAGAAACAGA GACTCCAAAG CTCAAGAAGA AGAAAAAGCC TAAGAAACCT 
CGGGACCCTA AAATCCCTAA GAGCAAGCGC CAAAAAAAGG AGCGTATGCT CTTATGCCGG 
CAGCTGGGGG ACAGCTCTGG GGAGGGGCCA GAGTTTGTGG AGGAGGAGGA AGAGGTGGCT 
CTGCGCTCAG ACAGTGAGGG CAGCGACTAT ACTCCTGGCA AGAAGAAGAA GAAGAAGCTT 
GGACCTAAGA AAGAGAAGAA GAGCAAATCC AAGCGGAAGG AGGAGGAGGA GGAGGAGGAT 
GATGATGATG ATTCAAAGGA GCCTAAATCA TCTGCTCAGC TCCTGGAAGA CTGGGGCATG 
GAAGACATTG ACCACGTGTT CTCAGAGGAG GATTATCGAA CCCTCACCAA CTACAAGGCC 
TTCAGCCAGT TTGTCAGACC CCTCATTGCT GCCAAAAATC CCAAGATTGC TGTCTCCAAG 
ATGATGATGG TTTTGGGTGC AAAATGGCGG GAGTTCAGTA CCAATAACCC CTTCAAAGGC 
AGTTCTGGGG CATCAGTGGC AGCTGCGGCA GCAGCAGCGG TAGCTGTGGT GGAGAGCATG 
GTGACAGCCA CTGAGGTTGC ACCACCACCT CCCCCTGTGG AGGTGCCTAT CCGCAAGGCC 
AAGACCAAGG AGGGCAAAGG TCCCAATGCT CGGAGGAAGC CCAAGGGCAG CCCTCGTGTA 
CCTGATGCCA AGAAGCCTAA ACCCAAGAAA GTAGCTCCCC TGAAAATCAA GCTGGGAGGT 
TTTGGTTCCA AGCGTAAGAG ATCCTCGAGT GAGGATGATG ACTTAGATGT GGAATCTGAC 
TTCGATGATG CCAGTATCAA TAGCTATTCT GTTTCTGATG GTTCCACCAG CCGTAGTAGC 
CGCAGCCGCA AGAAACTCCG AACCACTAAA AAGAAAAAGA AAGACCACCA GGACTATTGC 
GAGGTGTGCC AGCAAGGCGG TGAGATCATC CTGTGTGATA CCTGTCCCCG TGCTTACCAC 
ATGGTCTGCC TGGATCCCGA CATGGAGAAG GCTCCCGAGG GCAAGTGGAG CTGCCCACAC 
TGCGAGAAGG AAGGCATCCA GTGGGAAGCT AAAGAGGACA ATTCGGAGGG TGAGGAGATC 
CTGGAAGAGG TTGGGGGAGA CCTCGAAGAG GAGGATGACC ACCATATGGA ATTCTGTCGG 
GTCTGCAAGG ATGGTGGGGA ACTGCTCTGC TGTGATACCT GTCCTTCTTC CTACCACATC 
CACTGCCTGA ATCCCCCACT TCCAGAGATC CCCAACGGTG AATGGCTCTG TCCCCGTTGT 
ACGTGTCCAG CTCTGAAGGG CAAAGTGCAG AAGATCCTAA TCTGGAAGTG GGGTCAGCCA 
CCATCTCCCA CACCAGTGCC TCGGCCTCCA GATGCTGATC CCAACACGCC CTCCCCAAAG 
CCCTTGGAGG GGCGGCCAGA GCGGCAGTTC TTTGTGAAAT GGCAAGGCAT GTCTTACTGG 
CACTGCTCCT GGGTTTCTGA ACTGCAGCTG GAGCTGCACT GTCAGGTGAT GTTCCGAAAC 
TATCAGCGGA AGAATGATAT GGATGAGCCA CCTTCTGGGG ACTTTGGTGG TGATGAAGAG 
AAAAGCCGAA AGCGAAAGAA CAAGGACCCT AAATTTGCAG AGATGGAGGA ACGCTTCTAT 
CGCTATGGGA TAAAACCCGA GTGGATGATG ATCCACCGAA TCCTCAACCA CAGTGTGGAC 
AAGAAGGGCC ACGTCCACTA CTTGATCAAG TGGCGGGACT TACCTTACGA TCAGGCTTCT 
TGGGAGAGTG AGGATGTGGA GATCCAGGAT TACGACCTGT TCAAGCAGAG CTATTGGAAT 
CACAGGGAGT TAATGAGGGG TGAGGAAGGC CGACCAGGCA AGAAGCTCAA GAAGGTGAAG 
CTTCGGAAGT TGGAGAGGCC TCCAGAAACG CCAACAGTTG ATCCAACAGT GAAGTATGAG 
CGACAGCCAG AGTACCTGGA TGCTACAGGT GGAACCCTGC ACCCCTATCA AATGGAGGGC 
CTGAATTGGT TGCGCTTCTC CTGGGCTCAG GGCACTGACA CCATCTTGGC TGATGAGATG 
GGCCTTGGGA AAACTGTACA GACAGCAGTC TTCCTGTATT CCCTTTACAA GGAGGGTCAT 
TCCAAAGGCC CCTTCCTAGT GAGCGCCCCT CTTTCTACCA TCATCAACTG GGAGCGGGAG 
TTTGAAATGT GGGCTCCAGA CATGTATGTC GTAACCTATG TGGGTGACAA GGACAGCCGT 
GCCATCATCC GAGAGAATGA GTTCTCCTTT GAAGACAATG CCATTCGTGG TGGCAAGAAG 
GCCTCCCGCA TGAAGAAAGA GGCATCTGTG AAATTCCATG TGCTGCTGAC ATCCTATGAA 
TTGATCACCA TTGACATGGC TATTTTGGGC TCTATTGATT GGGCCTGCCT CATCGTGGAT 
GAAGCCCATC GGCTGAAGAA CAATCAGTCT AAGTTCTTCC GGGTATTGAA TGGTTACTCA 
CTCCAGCACA AGCTGTTGCT GACTGGGACA CCATTACAAA ACAATCTGGA AGAGTTGTTT 
CATCTGCTCA ACTTTCTCAC CCCCGAGAGG TTCCACAATT TGGAAGGTTT TTTGGAGGAG 
TTTGCTGACA TTGCCAAGGA GGACCAGATA AAAAAACTGC ATGACATGCT GGGGCCGCAC 
ATGTTGCGGC GGCTCAAAGC CGATGTGTTC AAGAACATGC CCTCCAAGAC AGAACTAATT 
GTGCGTGTGG AGCTGAGCCC TATGCAGAAG AAATACTACA AGTACATCCT CACTCGAAAT 
TTTGAAGCAC TCAATGCCCG AGGTGGTGGC AACCAGGTGT CTCTGCTGAA TGTGGTGATG 
GATCTTAAGA AGTGCTGCAA CCATCCATAC CTCTTCCCTG TGGCTGCAAT GGAAGCTCCT 
AAGATGCCTA ATGGCATGTA TGATGGCAGT GCCCTAATCA GAGCATCTGG GAAATTATTG 
CTGCTGCAGA AAATGCTCAA GAACCTTAAG GAGGGTGGGC ATCGTGTACT CATCTTTTCC 
CAGATGACCA AGATGCTAGA CCTGCTAGAG GATTTCTTGG AACATGAAGG TTATAAATAC 
GAACGCATCG ATGGTGGAAT CACTGGGAAC ATGCGGCAAG AGGCCATTGA CCGCTTCAAT 
GCACCGGGTG CTCAGCAGTT CTGCTTCTTG CTTTCCACTC GAGCTGGGGG CCTTGGAATC 
AATCTGGCCA CTGCTGACAC AGTTATTATC TATGACTCTG ACTGGAACCC CCATAATGAC 
ATTCAGGCCT TTAGCAGAGC TCACCGGATT GGGCAAAATA AAAAGGTAAT GATCTACCGG 
TTTGTGACCC GTGCGTCAGT GGAGGAGCGC ATCACGCAGG TGGCAAAGAA GAAAATGATG 
CTGACGCATC TAGTGGTGCG GCCTGGGCTG GGCTCCAAGA CTGGATCTAT GTCCAAACAG 
GAGCTTGATG ATATCCTCAA ATTTGGCACT GAGGAACTAT TCAAGGATGA AGCCACTGAT 
GGAGGAGGAG ACAACAAAGA GGGAGAAGAT AGCAGTGTTA TCCACTACGA TGATAAGGCC 
ATTGAACGGC TGCTAGACCG TAACCAGGAT GAGACTGAAG ACACAGAATT GCAGGGCATG 
AATGAATATT TGAGCTCATT CAAAGTGGCC CAGTATGTGG TACGGGAAGA AGAAATGGGG 
GAGGAAGAGG AGGTAGAACG GGAAATCATT AAACAGGAAG AAAGTGTGGA TCCTGACTAC 
TGGGAGAAAT TGCTGCGGCA CCATTATGAG CAGCAGCAAG AAGATCTAGC CCGAAATCTG 
GGCAAAGGAA AAAGAATCCG TAAACAGGTC AACTACAATG ATGGCTCCCA GGAGGACCGA 
GATTGGCAGG ACGACCAGTC CGACAACCAG TCCGATTACT CAGTGGCTTC AGAGGAAGGT 
GATGAAGACT TTGATGAACG TTCAGAAGCT CCCCGTAGGC CCAGTCGTAA GGGCCTGCGG 
AATGATAAAG ATAAGCCATT GCCTCCTCTG TTGGCCCGTG TTGGTGGGAA TATTGAAGTA 
CTTGGTTTTA ATGCTCGTCA GCGAAAAGCC TTTCTTAATG CAATTATGCG ATATGGTATG 
CCACCTCAGG ATGCTTTTAC TACCCAGTGG CTTGTAAGAG ACCTGCGAGG CAAATCAGAG 
AAAGAGTTCA AGGCATATGT CTCTCTTTTC ATGCGGCATT TATGTGAGCC GGGGGCAGAT 
GGGGCTGAGA CCTTTGCTGA TGGTGTCCCC CGAGAAGGCC TGTCTCGCCA GCATGTCCTT 
ACTAGAATTG GTGTTATGTC TTTGATTCGC AAGAAGGTTC AGGAGTTTGA ACATGTTAAT 
GGGCGCTGGA GCATGCCTGA ACTGGCTGAG GTGGAGGAAA ACAAGAAGAT GTCCCAGCCA 
GGGTCACCCT CCCCAAAAAC TCCTACACCC TCCACTCCAG GGGACACGCA GCCCAACACT 
CCTGCACCTG TCCCACCTGC TGAAGATGGG ATAAAAATAG AGGAAAATAG CCTCAAAGAA 
GAAGAGAGCA TAGAAGGAGA AAAGGAGGTT AAATCTACAG CCCCTGAGAC TGCCATTGAG 
TGTACACAGG CCCCTGCCCC TGCCTCAGAG GATGAAAAGG TCGTTGTTGA ACCCCCTGAG 
GGAGAGGAGA AAGTGGAAAA GGCAGAGGTG AAGGAGAGAA CAGAGGAACC TATGGAGACA 
GAGCCCAAAG GTAAAGGTGC TGCTGATGTA GAGAAGGTGG AGGAAAAGTC AGCAATAGAT 
CTGACCCCTA TTGTGGTAGA AGACAAAGAA GAGAAGAAAG AAGAAGAAGA GAAAAAAGAG 
GTGATGCTTC AGAATGGAGA GACCCCCAAG GACCTGAATG ATGAGAAACA GAAGAAAAAT 
ATTAAACAAC GTTTCATGTT TAACATTGCA GATGGTGGTT TTACTGAGTT GCACTCCCTT 
TGGCAGAATG AAGAGCGGGC AGCCACAGTT ACCAAGAAGA CTTATGAGAT CTGGCATCGA 
CGGCATGACT ACTGGCTGCT AGCCGGCATT ATAAACCATG GCTATGCCCG GTGGCAAGAC 
ATCCAGAATG ACCCACGCTA TGCCATCCTC AATGAGCCTT TCAAGGGTGA AATGAACCGT 
GGCAATTTCT TAGAGATCAA GAATAAATTT CTAGCTCGAA GGTTTAAGCT CTTAGAACAA 
GCTCTGGTGA TTGAGGAACA GCTGCGCCGG GCTGCTTACT TGAACATGTC AGAAGACCCT 
TCTCACCCTT CCATGGCCCT CAACACCCGC TTTGCTGAGG TGGAGTGTTT GGCGGAAAGT 
CATCAGCACC TGTCCAAGGA GTCAATGGCA GGAAACAAGC CAGCCAATGC AGTCCTGCAC 
AAAGGTATTC TGAAACAGCT GGAAGAACTG CTGAGTGACA TGAAAGCTGA TGTGACTCGA 
CTCCCAGCTA CCATTGCCCG AATTCCCCCA GTTGCTGTGA GGTTACAGAT GTCAGAGCGT 
AACATTCTCA GCCGCCTGGC AAACCGGGCA CCCGAACCTA CCCCACAGCA GGTAGCCCAG 
CAGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001350535.1
CDS	256..5964
Translation	MASGLGSPSPCSAGSEEEDMDALLNNSLPPPHPENEEDPEEDLSETETPKLKKKKKPKKPRDPKIPKSKRQKKERMLLCRQLGDSSGEGPEFVEEEEEVALRSDSEGSDYTPGKKKKKKLGPKKEKKSKSKRKEEEEEEDDDDDSKEPKSSAQLLEDWGMEDIDHVFSEEDYRTLTNYKAFSQFVRPLIAAKNPKIAVSKMMMVLGAKWREFSTNNPFKGSSGASVAAAAAAAVAVVESMVTATEVAPPPPPVEVPIRKAKTKEGKGPNARRKPKGSPRVPDAKKPKPKKVAPLKIKLGGFGSKRKRSSSEDDDLDVESDFDDASINSYSVSDGSTSRSSRSRKKLRTTKKKKKDHQDYCEVCQQGGEIILCDTCPRAYHMVCLDPDMEKAPEGKWSCPHCEKEGIQWEAKEDNSEGEEILEEVGGDLEEEDDHHMEFCRVCKDGGELLCCDTCPSSYHIHCLNPPLPEIPNGEWLCPRCTCPALKGKVQKILIWKWGQPPSPTPVPRPPDADPNTPSPKPLEGRPERQFFVKWQGMSYWHCSWVSELQLELHCQVMFRNYQRKNDMDEPPSGDFGGDEEKSRKRKNKDPKFAEMEERFYRYGIKPEWMMIHRILNHSVDKKGHVHYLIKWRDLPYDQASWESEDVEIQDYDLFKQSYWNHRELMRGEEGRPGKKLKKVKLRKLERPPETPTVDPTVKYERQPEYLDATGGTLHPYQMEGLNWLRFSWAQGTDTILADEMGLGKTVQTAVFLYSLYKEGHSKGPFLVSAPLSTIINWEREFEMWAPDMYVVTYVGDKDSRAIIRENEFSFEDNAIRGGKKASRMKKEASVKFHVLLTSYELITIDMAILGSIDWACLIVDEAHRLKNNQSKFFRVLNGYSLQHKLLLTGTPLQNNLEELFHLLNFLTPERFHNLEGFLEEFADIAKEDQIKKLHDMLGPHMLRRLKADVFKNMPSKTELIVRVELSPMQKKYYKYILTRNFEALNARGGGNQVSLLNVVMDLKKCCNHPYLFPVAAMEAPKMPNGMYDGSALIRASGKLLLLQKMLKNLKEGGHRVLIFSQMTKMLDLLEDFLEHEGYKYERIDGGITGNMRQEAIDRFNAPGAQQFCFLLSTRAGGLGINLATADTVIIYDSDWNPHNDIQAFSRAHRIGQNKKVMIYRFVTRASVEERITQVAKKKMMLTHLVVRPGLGSKTGSMSKQELDDILKFGTEELFKDEATDGGGDNKEGEDSSVIHYDDKAIERLLDRNQDETEDTELQGMNEYLSSFKVAQYVVREEEMGEEEEVEREIIKQEESVDPDYWEKLLRHHYEQQQEDLARNLGKGKRIRKQVNYNDGSQEDRDWQDDQSDNQSDYSVASEEGDEDFDERSEAPRRPSRKGLRNDKDKPLPPLLARVGGNIEVLGFNARQRKAFLNAIMRYGMPPQDAFTTQWLVRDLRGKSEKEFKAYVSLFMRHLCEPGADGAETFADGVPREGLSRQHVLTRIGVMSLIRKKVQEFEHVNGRWSMPELAEVEENKKMSQPGSPSPKTPTPSTPGDTQPNTPAPVPPAEDGIKIEENSLKEEESIEGEKEVKSTAPETAIECTQAPAPASEDEKVVVEPPEGEEKVEKAEVKERTEEPMETEPKGKGAADVEKVEEKSAIDLTPIVVEDKEEKKEEEEKKEVMLQNGETPKDLNDEKQKKNIKQRFMFNIADGGFTELHSLWQNEERAATVTKKTYEIWHRRHDYWLLAGIINHGYARWQDIQNDPRYAILNEPFKGEMNRGNFLEIKNKFLARRFKLLEQALVIEEQLRRAAYLNMSEDPSHPSMALNTRFAEVECLAESHQHLSKESMAGNKPANAVLHKGILKQLEELLSDMKADVTRLPATIARIPPVAVRLQMSERNILSRLANRAPEPTPQQVAQQQ

Target ORF information:

RefSeq Version	NM_001363606.1
Organism	Homo sapiens(human)
Definition	Homo sapiens chromodomain helicase DNA binding protein 4 (CHD4), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001363606.1

ORF Insert Sequence:

ATGGCGTCGG GCCTGGGCTC CCCGTCCCCC TGCTCGGCGG GCAGTGAGGA GGAGGATATG 
GATGCACTTT TGAACAACAG CCTGCCCCCA CCCCACCCAG AAAATGAAGA GGACCCAGAA 
GAGGATTTGT CAGAAACAGA GACTCCAAAG CTCAAGAAGA AGAAAAAGCC TAAGAAACCT 
CGGGACCCTA AAATCCCTAA GAGCAAGCGC CAAAAAAAGG AGCGTATGCT CTTATGCCGG 
CAGCTGGGGG ACAGCTCTGG GGAGGGGCCA GAGTTTGTGG AGGAGGAGGA AGAGGTGGCT 
CTGCGCTCAG ACAGTGAGGG CAGCGACTAT ACTCCTGGCA AGAAGAAGAA GAAGAAGCTT 
GGACCTAAGA AAGAGAAGAA GAGCAAATCC AAGCGGAAGG AGGAGGAGGA GGAGGAGGAT 
GATGATGATG ATTCAAAGGA GCCTAAATCA TCTGCTCAGC TCCTGGAAGA CTGGGGCATG 
GAAGACATTG ACCACGTGTT CTCAGAGGAG GATTATCGAA CCCTCACCAA CTACAAGGCC 
TTCAGCCAGT TTGTCAGACC CCTCATTGCT GCCAAAAATC CCAAGATTGC TGTCTCCAAG 
ATGATGATGG TTTTGGGTGC AAAATGGCGG GAGTTCAGTA CCAATAACCC CTTCAAAGGC 
AGTTCTGGGG CATCAGTGGC AGCTGCGGCA GCAGCAGCGG TAGCTGTGGT GGAGAGCATG 
GTGACAGCCA CTGAGGTTGC ACCACCACCT CCCCCTGTGG AGGTGCCTAT CCGCAAGGCC 
AAGACCAAGG AGGGCAAAGG TCCCAATGCT CGGAGGAAGC CCAAGGGCAG CCCTCGTGTA 
CCTGATGCCA AGAAGCCTAA ACCCAAGAAA GTAGCTCCCC TGAAAATCAA GCTGGGAGGT 
TTTGGTTCCA AGCGTAAGAG ATCCTCGAGT GAGGATGATG ACTTAGATGT GGAATCTGAC 
TTCGATGATG CCAGTATCAA TAGCTATTCT GTTTCTGATG GTTCCACCAG CCGTAGTAGC 
CGCAGCCGCA AGAAACTCCG AACCACTAAA AAGAAAAAGA AAGACCACCA GGACTATTGC 
GAGGTGTGCC AGCAAGGCGG TGAGATCATC CTGTGTGATA CCTGTCCCCG TGCTTACCAC 
ATGGTCTGCC TGGATCCCGA CATGGAGAAG GCTCCCGAGG GCAAGTGGAG CTGCCCACAC 
TGCGAGAAGG AAGGCATCCA GTGGGAAGCT AAAGAGGACA ATTCGGAGGG TGAGGAGATC 
CTGGAAGAGG TTGGGGGAGA CCTCGAAGAG GAGGATGACC ACCATATGGA ATTCTGTCGG 
GTCTGCAAGG ATGGTGGGGA ACTGCTCTGC TGTGATACCT GTCCTTCTTC CTACCACATC 
CACTGCCTGA ATCCCCCACT TCCAGAGATC CCCAACGGTG AATGGCTCTG TCCCCGTTGT 
ACGTGTCCAG CTCTGAAGGG CAAAGTGCAG AAGATCCTAA TCTGGAAGTG GGGTCAGCCA 
CCATCTCCCA CACCAGTGCC TCGGCCTCCA GATGCTGATC CCAACACGCC CTCCCCAAAG 
CCCTTGGAGG GGCGGCCAGA GCGGCAGTTC TTTGTGAAAT GGCAAGGCAT GTCTTACTGG 
CACTGCTCCT GGGTTTCTGA ACTGCAGCTG GAGCTGCACT GTCAGGTGAT GTTCCGAAAC 
TATCAGCGGA AGAATGATAT GGATGAGCCA CCTTCTGGGG ACTTTGGTGG TGATGAAGAG 
AAAAGCCGAA AGCGAAAGAA CAAGGACCCT AAATTTGCAG AGATGGAGGA ACGCTTCTAT 
CGCTATGGGA TAAAACCCGA GTGGATGATG ATCCACCGAA TCCTCAACCA CAGTGTGGAC 
AAGAAGGGCC ACGTCCACTA CTTGATCAAG TGGCGGGACT TACCTTACGA TCAGGCTTCT 
TGGGAGAGTG AGGATGTGGA GATCCAGGAT TACGACCTGT TCAAGCAGAG CTATTGGAAT 
CACAGGGAGT TAATGAGGGG TGAGGAAGGC CGACCAGGCA AGAAGCTCAA GAAGGTGAAG 
CTTCGGAAGT TGGAGAGGCC TCCAGAAACG CCAACAGTTG ATCCAACAGT GAAGTATGAG 
CGACAGCCAG AGTACCTGGA TGCTACAGGT GGAACCCTGC ACCCCTATCA AATGGAGGGC 
CTGAATTGGT TGCGCTTCTC CTGGGCTCAG GGCACTGACA CCATCTTGGC TGATGAGATG 
GGCCTTGGGA AAACTGTACA GACAGCAGTC TTCCTGTATT CCCTTTACAA GGAGGGTCAT 
TCCAAAGGCC CCTTCCTAGT GAGCGCCCCT CTTTCTACCA TCATCAACTG GGAGCGGGAG 
TTTGAAATGT GGGCTCCAGA CATGTATGTC GTAACCTATG TGGGTGACAA GGACAGCCGT 
GCCATCATCC GAGAGAATGA GTTCTCCTTT GAAGACAATG CCATTCGTGG TGGCAAGAAG 
GCCTCCCGCA TGAAGAAAGA GGCATCTGTG AAATTCCATG TGCTGCTGAC ATCCTATGAA 
TTGATCACCA TTGACATGGC TATTTTGGGC TCTATTGATT GGGCCTGCCT CATCGTGGAT 
GAAGCCCATC GGCTGAAGAA CAATCAGTCT AAGTTCTTCC GGGTATTGAA TGGTTACTCA 
CTCCAGCACA AGCTGTTGCT GACTGGGACA CCATTACAAA ACAATCTGGA AGAGTTGTTT 
CATCTGCTCA ACTTTCTCAC CCCCGAGAGG TTCCACAATT TGGAAGGTTT TTTGGAGGAG 
TTTGCTGACA TTGCCAAGGA GGACCAGATA AAAAAACTGC ATGACATGCT GGGGCCGCAC 
ATGTTGCGGC GGCTCAAAGC CGATGTGTTC AAGAACATGC CCTCCAAGAC AGAACTAATT 
GTGCGTGTGG AGCTGAGCCC TATGCAGAAG AAATACTACA AGTACATCCT CACTCGAAAT 
TTTGAAGCAC TCAATGCCCG AGGTGGTGGC AACCAGGTGT CTCTGCTGAA TGTGGTGATG 
GATCTTAAGA AGTGCTGCAA CCATCCATAC CTCTTCCCTG TGGCTGCAAT GGAAGCTCCT 
AAGATGCCTA ATGGCATGTA TGATGGCAGT GCCCTAATCA GAGCATCTGG GAAATTATTG 
CTGCTGCAGA AAATGCTCAA GAACCTTAAG GAGGGTGGGC ATCGTGTACT CATCTTTTCC 
CAGATGACCA AGATGCTAGA CCTGCTAGAG GATTTCTTGG AACATGAAGG TTATAAATAC 
GAACGCATCG ATGGTGGAAT CACTGGGAAC ATGCGGCAAG AGGCCATTGA CCGCTTCAAT 
GCACCGGGTG CTCAGCAGTT CTGCTTCTTG CTTTCCACTC GAGCTGGGGG CCTTGGAATC 
AATCTGGCCA CTGCTGACAC AGTTATTATC TATGACTCTG ACTGGAACCC CCATAATGAC 
ATTCAGGCCT TTAGCAGAGC TCACCGGATT GGGCAAAATA AAAAGGTAAT GATCTACCGG 
TTTGTGACCC GTGCGTCAGT GGAGGAGCGC ATCACGCAGG TGGCAAAGAA GAAAATGATG 
CTGACGCATC TAGTGGTGCG GCCTGGGCTG GGCTCCAAGA CTGGATCTAT GTCCAAACAG 
GAGCTTGATG ATATCCTCAA ATTTGGCACT GAGGAACTAT TCAAGGATGA AGCCACTGAT 
GGAGGAGGAG ACAACAAAGA GGGAGAAGAT AGCAGTGTTA TCCACTACGA TGATAAGGCC 
ATTGAACGGC TGCTAGACCG TAACCAGGAT GAGACTGAAG ACACAGAATT GCAGGGCATG 
AATGAATATT TGAGCTCATT CAAAGTGGCC CAGTATGTGG TACGGGAAGA AGAAATGGGG 
GAGGAAGAGG AGGTAGAACG GGAAATCATT AAACAGGAAG AAAGTGTGGA TCCTGACTAC 
TGGGAGAAAT TGCTGCGGCA CCATTATGAG CAGCAGCAAG AAGATCTAGC CCGAAATCTG 
GGCAAAGGAA AAAGAATCCG TAAACAGGTC AACTACAATG ATGGCTCCCA GGAGGACCGA 
GATTGGCAGG ACGACCAGTC CGACAACCAG TCCGATTACT CAGTGGCTTC AGAGGAAGGT 
GATGAAGACT TTGATGAACG TTCAGAAGCT CCCCGTAGGC CCAGTCGTAA GGGCCTGCGG 
AATGATAAAG ATAAGCCATT GCCTCCTCTG TTGGCCCGTG TTGGTGGGAA TATTGAAGTA 
CTTGGTTTTA ATGCTCGTCA GCGAAAAGCC TTTCTTAATG CAATTATGCG ATATGGTATG 
CCACCTCAGG ATGCTTTTAC TACCCAGTGG CTTGTAAGAG ACCTGCGAGG CAAATCAGAG 
AAAGAGTTCA AGGCATATGT CTCTCTTTTC ATGCGGCATT TATGTGAGCC GGGGGCAGAT 
GGGGCTGAGA CCTTTGCTGA TGGTGTCCCC CGAGAAGGCC TGTCTCGCCA GCATGTCCTT 
ACTAGAATTG GTGTTATGTC TTTGATTCGC AAGAAGGTTC AGGAGTTTGA ACATGTTAAT 
GGGCGCTGGA GCATGCCTGA ACTGGCTGAG GTGGAGGAAA ACAAGAAGAT GTCCCAGCCA 
GGGTCACCCT CCCCAAAAAC TCCTACACCC TCCACTCCAG GGGACACGCA GCCCAACACT 
CCTGCACCTG TCCCACCTGC TGAAGATGGG ATAAAAATAG AGGAAAATAG CCTCAAAGAA 
GAAGAGAGCA TAGAAGGAGA AAAGGAGGTT AAATCTACAG CCCCTGAGAC TGCCATTGAG 
TGTACACAGG CCCCTGCCCC TGCCTCAGAG GATGAAAAGG TCGTTGTTGA ACCCCCTGAG 
GGAGAGGAGA AAGTGGAAAA GGCAGAGGTG AAGGAGAGAA CAGAGGAACC TATGGAGACA 
GAGCCCAAAG GTAAAGGTGC TGCTGATGTA GAGAAGGTGG AGGAAAAGTC AGCAATAGAT 
CTGACCCCTA TTGTGGTAGA AGACAAAGAA GAGAAGAAAG AAGAAGAAGA GAAAAAAGAG 
GTGATGCTTC AGAATGGAGA GACCCCCAAG GACCTGAATG ATGAGAAACA GAAGAAAAAT 
ATTAAACAAC GTTTCATGTT TAACATTGCA GATGGTGGTT TTACTGAGTT GCACTCCCTT 
TGGCAGAATG AAGAGCGGGC AGCCACAGTT ACCAAGAAGA CTTATGAGAT CTGGCATCGA 
CGGCATGACT ACTGGCTGCT AGCCGGCATT ATAAACCATG GCTATGCCCG GTGGCAAGAC 
ATCCAGAATG ACCCACGCTA TGCCATCCTC AATGAGCCTT TCAAGGGTGA AATGAACCGT 
GGCAATTTCT TAGAGATCAA GAATAAATTT CTAGCTCGAA GGTTTAAGCT CTTAGAACAA 
GCTCTGGTGA TTGAGGAACA GCTGCGCCGG GCTGCTTACT TGAACATGTC AGAAGACCCT 
TCTCACCCTT CCATGGCCCT CAACACCCGC TTTGCTGAGG TGGAGTGTTT GGCGGAAAGT 
CATCAGCACC TGTCCAAGGA GTCAATGGCA GGAAACAAGC CAGCCAATGC AGTCCTGCAC 
AAAGGTATTC TGAAACAGCT GGAAGAACTG CTGAGTGACA TGAAAGCTGA TGTGACTCGA 
CTCCCAGCTA CCATTGCCCG AATTCCCCCA GTTGCTGTGA GGTTACAGAT GTCAGAGCGT 
AACATTCTCA GCCGCCTGGC AAACCGGGCA CCCGAACCTA CCCCACAGCA GGTAGCCCAG 
CAGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHD4 ( NM_001363606.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001350535.1 Homo sapiens chromodomain helicase DNA binding protein 4 (CHD4), transcript variant 3, mRNA.

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CloneID	OHu45954
Clone ID Related Accession (Same CDS sequence)	XM_006718962.1 , NM_001363606.1 , NM_001363606.2
Accession Version	NM_001363606.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5709bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Homo sapiens(human)
Product	chromodomain-helicase-DNA-binding protein 4 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC006064.10. On Jun 2, 2019 this sequence version replaced NM_001363606.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.258631.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGGCGTCGG GCCTGGGCTC CCCGTCCCCC TGCTCGGCGG GCAGTGAGGA GGAGGATATG 
GATGCACTTT TGAACAACAG CCTGCCCCCA CCCCACCCAG AAAATGAAGA GGACCCAGAA 
GAGGATTTGT CAGAAACAGA GACTCCAAAG CTCAAGAAGA AGAAAAAGCC TAAGAAACCT 
CGGGACCCTA AAATCCCTAA GAGCAAGCGC CAAAAAAAGG AGCGTATGCT CTTATGCCGG 
CAGCTGGGGG ACAGCTCTGG GGAGGGGCCA GAGTTTGTGG AGGAGGAGGA AGAGGTGGCT 
CTGCGCTCAG ACAGTGAGGG CAGCGACTAT ACTCCTGGCA AGAAGAAGAA GAAGAAGCTT 
GGACCTAAGA AAGAGAAGAA GAGCAAATCC AAGCGGAAGG AGGAGGAGGA GGAGGAGGAT 
GATGATGATG ATTCAAAGGA GCCTAAATCA TCTGCTCAGC TCCTGGAAGA CTGGGGCATG 
GAAGACATTG ACCACGTGTT CTCAGAGGAG GATTATCGAA CCCTCACCAA CTACAAGGCC 
TTCAGCCAGT TTGTCAGACC CCTCATTGCT GCCAAAAATC CCAAGATTGC TGTCTCCAAG 
ATGATGATGG TTTTGGGTGC AAAATGGCGG GAGTTCAGTA CCAATAACCC CTTCAAAGGC 
AGTTCTGGGG CATCAGTGGC AGCTGCGGCA GCAGCAGCGG TAGCTGTGGT GGAGAGCATG 
GTGACAGCCA CTGAGGTTGC ACCACCACCT CCCCCTGTGG AGGTGCCTAT CCGCAAGGCC 
AAGACCAAGG AGGGCAAAGG TCCCAATGCT CGGAGGAAGC CCAAGGGCAG CCCTCGTGTA 
CCTGATGCCA AGAAGCCTAA ACCCAAGAAA GTAGCTCCCC TGAAAATCAA GCTGGGAGGT 
TTTGGTTCCA AGCGTAAGAG ATCCTCGAGT GAGGATGATG ACTTAGATGT GGAATCTGAC 
TTCGATGATG CCAGTATCAA TAGCTATTCT GTTTCTGATG GTTCCACCAG CCGTAGTAGC 
CGCAGCCGCA AGAAACTCCG AACCACTAAA AAGAAAAAGA AAGACCACCA GGACTATTGC 
GAGGTGTGCC AGCAAGGCGG TGAGATCATC CTGTGTGATA CCTGTCCCCG TGCTTACCAC 
ATGGTCTGCC TGGATCCCGA CATGGAGAAG GCTCCCGAGG GCAAGTGGAG CTGCCCACAC 
TGCGAGAAGG AAGGCATCCA GTGGGAAGCT AAAGAGGACA ATTCGGAGGG TGAGGAGATC 
CTGGAAGAGG TTGGGGGAGA CCTCGAAGAG GAGGATGACC ACCATATGGA ATTCTGTCGG 
GTCTGCAAGG ATGGTGGGGA ACTGCTCTGC TGTGATACCT GTCCTTCTTC CTACCACATC 
CACTGCCTGA ATCCCCCACT TCCAGAGATC CCCAACGGTG AATGGCTCTG TCCCCGTTGT 
ACGTGTCCAG CTCTGAAGGG CAAAGTGCAG AAGATCCTAA TCTGGAAGTG GGGTCAGCCA 
CCATCTCCCA CACCAGTGCC TCGGCCTCCA GATGCTGATC CCAACACGCC CTCCCCAAAG 
CCCTTGGAGG GGCGGCCAGA GCGGCAGTTC TTTGTGAAAT GGCAAGGCAT GTCTTACTGG 
CACTGCTCCT GGGTTTCTGA ACTGCAGCTG GAGCTGCACT GTCAGGTGAT GTTCCGAAAC 
TATCAGCGGA AGAATGATAT GGATGAGCCA CCTTCTGGGG ACTTTGGTGG TGATGAAGAG 
AAAAGCCGAA AGCGAAAGAA CAAGGACCCT AAATTTGCAG AGATGGAGGA ACGCTTCTAT 
CGCTATGGGA TAAAACCCGA GTGGATGATG ATCCACCGAA TCCTCAACCA CAGTGTGGAC 
AAGAAGGGCC ACGTCCACTA CTTGATCAAG TGGCGGGACT TACCTTACGA TCAGGCTTCT 
TGGGAGAGTG AGGATGTGGA GATCCAGGAT TACGACCTGT TCAAGCAGAG CTATTGGAAT 
CACAGGGAGT TAATGAGGGG TGAGGAAGGC CGACCAGGCA AGAAGCTCAA GAAGGTGAAG 
CTTCGGAAGT TGGAGAGGCC TCCAGAAACG CCAACAGTTG ATCCAACAGT GAAGTATGAG 
CGACAGCCAG AGTACCTGGA TGCTACAGGT GGAACCCTGC ACCCCTATCA AATGGAGGGC 
CTGAATTGGT TGCGCTTCTC CTGGGCTCAG GGCACTGACA CCATCTTGGC TGATGAGATG 
GGCCTTGGGA AAACTGTACA GACAGCAGTC TTCCTGTATT CCCTTTACAA GGAGGGTCAT 
TCCAAAGGCC CCTTCCTAGT GAGCGCCCCT CTTTCTACCA TCATCAACTG GGAGCGGGAG 
TTTGAAATGT GGGCTCCAGA CATGTATGTC GTAACCTATG TGGGTGACAA GGACAGCCGT 
GCCATCATCC GAGAGAATGA GTTCTCCTTT GAAGACAATG CCATTCGTGG TGGCAAGAAG 
GCCTCCCGCA TGAAGAAAGA GGCATCTGTG AAATTCCATG TGCTGCTGAC ATCCTATGAA 
TTGATCACCA TTGACATGGC TATTTTGGGC TCTATTGATT GGGCCTGCCT CATCGTGGAT 
GAAGCCCATC GGCTGAAGAA CAATCAGTCT AAGTTCTTCC GGGTATTGAA TGGTTACTCA 
CTCCAGCACA AGCTGTTGCT GACTGGGACA CCATTACAAA ACAATCTGGA AGAGTTGTTT 
CATCTGCTCA ACTTTCTCAC CCCCGAGAGG TTCCACAATT TGGAAGGTTT TTTGGAGGAG 
TTTGCTGACA TTGCCAAGGA GGACCAGATA AAAAAACTGC ATGACATGCT GGGGCCGCAC 
ATGTTGCGGC GGCTCAAAGC CGATGTGTTC AAGAACATGC CCTCCAAGAC AGAACTAATT 
GTGCGTGTGG AGCTGAGCCC TATGCAGAAG AAATACTACA AGTACATCCT CACTCGAAAT 
TTTGAAGCAC TCAATGCCCG AGGTGGTGGC AACCAGGTGT CTCTGCTGAA TGTGGTGATG 
GATCTTAAGA AGTGCTGCAA CCATCCATAC CTCTTCCCTG TGGCTGCAAT GGAAGCTCCT 
AAGATGCCTA ATGGCATGTA TGATGGCAGT GCCCTAATCA GAGCATCTGG GAAATTATTG 
CTGCTGCAGA AAATGCTCAA GAACCTTAAG GAGGGTGGGC ATCGTGTACT CATCTTTTCC 
CAGATGACCA AGATGCTAGA CCTGCTAGAG GATTTCTTGG AACATGAAGG TTATAAATAC 
GAACGCATCG ATGGTGGAAT CACTGGGAAC ATGCGGCAAG AGGCCATTGA CCGCTTCAAT 
GCACCGGGTG CTCAGCAGTT CTGCTTCTTG CTTTCCACTC GAGCTGGGGG CCTTGGAATC 
AATCTGGCCA CTGCTGACAC AGTTATTATC TATGACTCTG ACTGGAACCC CCATAATGAC 
ATTCAGGCCT TTAGCAGAGC TCACCGGATT GGGCAAAATA AAAAGGTAAT GATCTACCGG 
TTTGTGACCC GTGCGTCAGT GGAGGAGCGC ATCACGCAGG TGGCAAAGAA GAAAATGATG 
CTGACGCATC TAGTGGTGCG GCCTGGGCTG GGCTCCAAGA CTGGATCTAT GTCCAAACAG 
GAGCTTGATG ATATCCTCAA ATTTGGCACT GAGGAACTAT TCAAGGATGA AGCCACTGAT 
GGAGGAGGAG ACAACAAAGA GGGAGAAGAT AGCAGTGTTA TCCACTACGA TGATAAGGCC 
ATTGAACGGC TGCTAGACCG TAACCAGGAT GAGACTGAAG ACACAGAATT GCAGGGCATG 
AATGAATATT TGAGCTCATT CAAAGTGGCC CAGTATGTGG TACGGGAAGA AGAAATGGGG 
GAGGAAGAGG AGGTAGAACG GGAAATCATT AAACAGGAAG AAAGTGTGGA TCCTGACTAC 
TGGGAGAAAT TGCTGCGGCA CCATTATGAG CAGCAGCAAG AAGATCTAGC CCGAAATCTG 
GGCAAAGGAA AAAGAATCCG TAAACAGGTC AACTACAATG ATGGCTCCCA GGAGGACCGA 
GATTGGCAGG ACGACCAGTC CGACAACCAG TCCGATTACT CAGTGGCTTC AGAGGAAGGT 
GATGAAGACT TTGATGAACG TTCAGAAGCT CCCCGTAGGC CCAGTCGTAA GGGCCTGCGG 
AATGATAAAG ATAAGCCATT GCCTCCTCTG TTGGCCCGTG TTGGTGGGAA TATTGAAGTA 
CTTGGTTTTA ATGCTCGTCA GCGAAAAGCC TTTCTTAATG CAATTATGCG ATATGGTATG 
CCACCTCAGG ATGCTTTTAC TACCCAGTGG CTTGTAAGAG ACCTGCGAGG CAAATCAGAG 
AAAGAGTTCA AGGCATATGT CTCTCTTTTC ATGCGGCATT TATGTGAGCC GGGGGCAGAT 
GGGGCTGAGA CCTTTGCTGA TGGTGTCCCC CGAGAAGGCC TGTCTCGCCA GCATGTCCTT 
ACTAGAATTG GTGTTATGTC TTTGATTCGC AAGAAGGTTC AGGAGTTTGA ACATGTTAAT 
GGGCGCTGGA GCATGCCTGA ACTGGCTGAG GTGGAGGAAA ACAAGAAGAT GTCCCAGCCA 
GGGTCACCCT CCCCAAAAAC TCCTACACCC TCCACTCCAG GGGACACGCA GCCCAACACT 
CCTGCACCTG TCCCACCTGC TGAAGATGGG ATAAAAATAG AGGAAAATAG CCTCAAAGAA 
GAAGAGAGCA TAGAAGGAGA AAAGGAGGTT AAATCTACAG CCCCTGAGAC TGCCATTGAG 
TGTACACAGG CCCCTGCCCC TGCCTCAGAG GATGAAAAGG TCGTTGTTGA ACCCCCTGAG 
GGAGAGGAGA AAGTGGAAAA GGCAGAGGTG AAGGAGAGAA CAGAGGAACC TATGGAGACA 
GAGCCCAAAG GTAAAGGTGC TGCTGATGTA GAGAAGGTGG AGGAAAAGTC AGCAATAGAT 
CTGACCCCTA TTGTGGTAGA AGACAAAGAA GAGAAGAAAG AAGAAGAAGA GAAAAAAGAG 
GTGATGCTTC AGAATGGAGA GACCCCCAAG GACCTGAATG ATGAGAAACA GAAGAAAAAT 
ATTAAACAAC GTTTCATGTT TAACATTGCA GATGGTGGTT TTACTGAGTT GCACTCCCTT 
TGGCAGAATG AAGAGCGGGC AGCCACAGTT ACCAAGAAGA CTTATGAGAT CTGGCATCGA 
CGGCATGACT ACTGGCTGCT AGCCGGCATT ATAAACCATG GCTATGCCCG GTGGCAAGAC 
ATCCAGAATG ACCCACGCTA TGCCATCCTC AATGAGCCTT TCAAGGGTGA AATGAACCGT 
GGCAATTTCT TAGAGATCAA GAATAAATTT CTAGCTCGAA GGTTTAAGCT CTTAGAACAA 
GCTCTGGTGA TTGAGGAACA GCTGCGCCGG GCTGCTTACT TGAACATGTC AGAAGACCCT 
TCTCACCCTT CCATGGCCCT CAACACCCGC TTTGCTGAGG TGGAGTGTTT GGCGGAAAGT 
CATCAGCACC TGTCCAAGGA GTCAATGGCA GGAAACAAGC CAGCCAATGC AGTCCTGCAC 
AAAGGTATTC TGAAACAGCT GGAAGAACTG CTGAGTGACA TGAAAGCTGA TGTGACTCGA 
CTCCCAGCTA CCATTGCCCG AATTCCCCCA GTTGCTGTGA GGTTACAGAT GTCAGAGCGT 
AACATTCTCA GCCGCCTGGC AAACCGGGCA CCCGAACCTA CCCCACAGCA GGTAGCCCAG 
CAGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001350535.1
CDS	159..5867
Translation	MASGLGSPSPCSAGSEEEDMDALLNNSLPPPHPENEEDPEEDLSETETPKLKKKKKPKKPRDPKIPKSKRQKKERMLLCRQLGDSSGEGPEFVEEEEEVALRSDSEGSDYTPGKKKKKKLGPKKEKKSKSKRKEEEEEEDDDDDSKEPKSSAQLLEDWGMEDIDHVFSEEDYRTLTNYKAFSQFVRPLIAAKNPKIAVSKMMMVLGAKWREFSTNNPFKGSSGASVAAAAAAAVAVVESMVTATEVAPPPPPVEVPIRKAKTKEGKGPNARRKPKGSPRVPDAKKPKPKKVAPLKIKLGGFGSKRKRSSSEDDDLDVESDFDDASINSYSVSDGSTSRSSRSRKKLRTTKKKKKDHQDYCEVCQQGGEIILCDTCPRAYHMVCLDPDMEKAPEGKWSCPHCEKEGIQWEAKEDNSEGEEILEEVGGDLEEEDDHHMEFCRVCKDGGELLCCDTCPSSYHIHCLNPPLPEIPNGEWLCPRCTCPALKGKVQKILIWKWGQPPSPTPVPRPPDADPNTPSPKPLEGRPERQFFVKWQGMSYWHCSWVSELQLELHCQVMFRNYQRKNDMDEPPSGDFGGDEEKSRKRKNKDPKFAEMEERFYRYGIKPEWMMIHRILNHSVDKKGHVHYLIKWRDLPYDQASWESEDVEIQDYDLFKQSYWNHRELMRGEEGRPGKKLKKVKLRKLERPPETPTVDPTVKYERQPEYLDATGGTLHPYQMEGLNWLRFSWAQGTDTILADEMGLGKTVQTAVFLYSLYKEGHSKGPFLVSAPLSTIINWEREFEMWAPDMYVVTYVGDKDSRAIIRENEFSFEDNAIRGGKKASRMKKEASVKFHVLLTSYELITIDMAILGSIDWACLIVDEAHRLKNNQSKFFRVLNGYSLQHKLLLTGTPLQNNLEELFHLLNFLTPERFHNLEGFLEEFADIAKEDQIKKLHDMLGPHMLRRLKADVFKNMPSKTELIVRVELSPMQKKYYKYILTRNFEALNARGGGNQVSLLNVVMDLKKCCNHPYLFPVAAMEAPKMPNGMYDGSALIRASGKLLLLQKMLKNLKEGGHRVLIFSQMTKMLDLLEDFLEHEGYKYERIDGGITGNMRQEAIDRFNAPGAQQFCFLLSTRAGGLGINLATADTVIIYDSDWNPHNDIQAFSRAHRIGQNKKVMIYRFVTRASVEERITQVAKKKMMLTHLVVRPGLGSKTGSMSKQELDDILKFGTEELFKDEATDGGGDNKEGEDSSVIHYDDKAIERLLDRNQDETEDTELQGMNEYLSSFKVAQYVVREEEMGEEEEVEREIIKQEESVDPDYWEKLLRHHYEQQQEDLARNLGKGKRIRKQVNYNDGSQEDRDWQDDQSDNQSDYSVASEEGDEDFDERSEAPRRPSRKGLRNDKDKPLPPLLARVGGNIEVLGFNARQRKAFLNAIMRYGMPPQDAFTTQWLVRDLRGKSEKEFKAYVSLFMRHLCEPGADGAETFADGVPREGLSRQHVLTRIGVMSLIRKKVQEFEHVNGRWSMPELAEVEENKKMSQPGSPSPKTPTPSTPGDTQPNTPAPVPPAEDGIKIEENSLKEEESIEGEKEVKSTAPETAIECTQAPAPASEDEKVVVEPPEGEEKVEKAEVKERTEEPMETEPKGKGAADVEKVEEKSAIDLTPIVVEDKEEKKEEEEKKEVMLQNGETPKDLNDEKQKKNIKQRFMFNIADGGFTELHSLWQNEERAATVTKKTYEIWHRRHDYWLLAGIINHGYARWQDIQNDPRYAILNEPFKGEMNRGNFLEIKNKFLARRFKLLEQALVIEEQLRRAAYLNMSEDPSHPSMALNTRFAEVECLAESHQHLSKESMAGNKPANAVLHKGILKQLEELLSDMKADVTRLPATIARIPPVAVRLQMSERNILSRLANRAPEPTPQQVAQQQ

Target ORF information:

RefSeq Version	NM_001363606.2
Organism	Homo sapiens(human)
Definition	Homo sapiens chromodomain helicase DNA binding protein 4 (CHD4), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001363606.2

ORF Insert Sequence:

ATGGCGTCGG GCCTGGGCTC CCCGTCCCCC TGCTCGGCGG GCAGTGAGGA GGAGGATATG 
GATGCACTTT TGAACAACAG CCTGCCCCCA CCCCACCCAG AAAATGAAGA GGACCCAGAA 
GAGGATTTGT CAGAAACAGA GACTCCAAAG CTCAAGAAGA AGAAAAAGCC TAAGAAACCT 
CGGGACCCTA AAATCCCTAA GAGCAAGCGC CAAAAAAAGG AGCGTATGCT CTTATGCCGG 
CAGCTGGGGG ACAGCTCTGG GGAGGGGCCA GAGTTTGTGG AGGAGGAGGA AGAGGTGGCT 
CTGCGCTCAG ACAGTGAGGG CAGCGACTAT ACTCCTGGCA AGAAGAAGAA GAAGAAGCTT 
GGACCTAAGA AAGAGAAGAA GAGCAAATCC AAGCGGAAGG AGGAGGAGGA GGAGGAGGAT 
GATGATGATG ATTCAAAGGA GCCTAAATCA TCTGCTCAGC TCCTGGAAGA CTGGGGCATG 
GAAGACATTG ACCACGTGTT CTCAGAGGAG GATTATCGAA CCCTCACCAA CTACAAGGCC 
TTCAGCCAGT TTGTCAGACC CCTCATTGCT GCCAAAAATC CCAAGATTGC TGTCTCCAAG 
ATGATGATGG TTTTGGGTGC AAAATGGCGG GAGTTCAGTA CCAATAACCC CTTCAAAGGC 
AGTTCTGGGG CATCAGTGGC AGCTGCGGCA GCAGCAGCGG TAGCTGTGGT GGAGAGCATG 
GTGACAGCCA CTGAGGTTGC ACCACCACCT CCCCCTGTGG AGGTGCCTAT CCGCAAGGCC 
AAGACCAAGG AGGGCAAAGG TCCCAATGCT CGGAGGAAGC CCAAGGGCAG CCCTCGTGTA 
CCTGATGCCA AGAAGCCTAA ACCCAAGAAA GTAGCTCCCC TGAAAATCAA GCTGGGAGGT 
TTTGGTTCCA AGCGTAAGAG ATCCTCGAGT GAGGATGATG ACTTAGATGT GGAATCTGAC 
TTCGATGATG CCAGTATCAA TAGCTATTCT GTTTCTGATG GTTCCACCAG CCGTAGTAGC 
CGCAGCCGCA AGAAACTCCG AACCACTAAA AAGAAAAAGA AAGACCACCA GGACTATTGC 
GAGGTGTGCC AGCAAGGCGG TGAGATCATC CTGTGTGATA CCTGTCCCCG TGCTTACCAC 
ATGGTCTGCC TGGATCCCGA CATGGAGAAG GCTCCCGAGG GCAAGTGGAG CTGCCCACAC 
TGCGAGAAGG AAGGCATCCA GTGGGAAGCT AAAGAGGACA ATTCGGAGGG TGAGGAGATC 
CTGGAAGAGG TTGGGGGAGA CCTCGAAGAG GAGGATGACC ACCATATGGA ATTCTGTCGG 
GTCTGCAAGG ATGGTGGGGA ACTGCTCTGC TGTGATACCT GTCCTTCTTC CTACCACATC 
CACTGCCTGA ATCCCCCACT TCCAGAGATC CCCAACGGTG AATGGCTCTG TCCCCGTTGT 
ACGTGTCCAG CTCTGAAGGG CAAAGTGCAG AAGATCCTAA TCTGGAAGTG GGGTCAGCCA 
CCATCTCCCA CACCAGTGCC TCGGCCTCCA GATGCTGATC CCAACACGCC CTCCCCAAAG 
CCCTTGGAGG GGCGGCCAGA GCGGCAGTTC TTTGTGAAAT GGCAAGGCAT GTCTTACTGG 
CACTGCTCCT GGGTTTCTGA ACTGCAGCTG GAGCTGCACT GTCAGGTGAT GTTCCGAAAC 
TATCAGCGGA AGAATGATAT GGATGAGCCA CCTTCTGGGG ACTTTGGTGG TGATGAAGAG 
AAAAGCCGAA AGCGAAAGAA CAAGGACCCT AAATTTGCAG AGATGGAGGA ACGCTTCTAT 
CGCTATGGGA TAAAACCCGA GTGGATGATG ATCCACCGAA TCCTCAACCA CAGTGTGGAC 
AAGAAGGGCC ACGTCCACTA CTTGATCAAG TGGCGGGACT TACCTTACGA TCAGGCTTCT 
TGGGAGAGTG AGGATGTGGA GATCCAGGAT TACGACCTGT TCAAGCAGAG CTATTGGAAT 
CACAGGGAGT TAATGAGGGG TGAGGAAGGC CGACCAGGCA AGAAGCTCAA GAAGGTGAAG 
CTTCGGAAGT TGGAGAGGCC TCCAGAAACG CCAACAGTTG ATCCAACAGT GAAGTATGAG 
CGACAGCCAG AGTACCTGGA TGCTACAGGT GGAACCCTGC ACCCCTATCA AATGGAGGGC 
CTGAATTGGT TGCGCTTCTC CTGGGCTCAG GGCACTGACA CCATCTTGGC TGATGAGATG 
GGCCTTGGGA AAACTGTACA GACAGCAGTC TTCCTGTATT CCCTTTACAA GGAGGGTCAT 
TCCAAAGGCC CCTTCCTAGT GAGCGCCCCT CTTTCTACCA TCATCAACTG GGAGCGGGAG 
TTTGAAATGT GGGCTCCAGA CATGTATGTC GTAACCTATG TGGGTGACAA GGACAGCCGT 
GCCATCATCC GAGAGAATGA GTTCTCCTTT GAAGACAATG CCATTCGTGG TGGCAAGAAG 
GCCTCCCGCA TGAAGAAAGA GGCATCTGTG AAATTCCATG TGCTGCTGAC ATCCTATGAA 
TTGATCACCA TTGACATGGC TATTTTGGGC TCTATTGATT GGGCCTGCCT CATCGTGGAT 
GAAGCCCATC GGCTGAAGAA CAATCAGTCT AAGTTCTTCC GGGTATTGAA TGGTTACTCA 
CTCCAGCACA AGCTGTTGCT GACTGGGACA CCATTACAAA ACAATCTGGA AGAGTTGTTT 
CATCTGCTCA ACTTTCTCAC CCCCGAGAGG TTCCACAATT TGGAAGGTTT TTTGGAGGAG 
TTTGCTGACA TTGCCAAGGA GGACCAGATA AAAAAACTGC ATGACATGCT GGGGCCGCAC 
ATGTTGCGGC GGCTCAAAGC CGATGTGTTC AAGAACATGC CCTCCAAGAC AGAACTAATT 
GTGCGTGTGG AGCTGAGCCC TATGCAGAAG AAATACTACA AGTACATCCT CACTCGAAAT 
TTTGAAGCAC TCAATGCCCG AGGTGGTGGC AACCAGGTGT CTCTGCTGAA TGTGGTGATG 
GATCTTAAGA AGTGCTGCAA CCATCCATAC CTCTTCCCTG TGGCTGCAAT GGAAGCTCCT 
AAGATGCCTA ATGGCATGTA TGATGGCAGT GCCCTAATCA GAGCATCTGG GAAATTATTG 
CTGCTGCAGA AAATGCTCAA GAACCTTAAG GAGGGTGGGC ATCGTGTACT CATCTTTTCC 
CAGATGACCA AGATGCTAGA CCTGCTAGAG GATTTCTTGG AACATGAAGG TTATAAATAC 
GAACGCATCG ATGGTGGAAT CACTGGGAAC ATGCGGCAAG AGGCCATTGA CCGCTTCAAT 
GCACCGGGTG CTCAGCAGTT CTGCTTCTTG CTTTCCACTC GAGCTGGGGG CCTTGGAATC 
AATCTGGCCA CTGCTGACAC AGTTATTATC TATGACTCTG ACTGGAACCC CCATAATGAC 
ATTCAGGCCT TTAGCAGAGC TCACCGGATT GGGCAAAATA AAAAGGTAAT GATCTACCGG 
TTTGTGACCC GTGCGTCAGT GGAGGAGCGC ATCACGCAGG TGGCAAAGAA GAAAATGATG 
CTGACGCATC TAGTGGTGCG GCCTGGGCTG GGCTCCAAGA CTGGATCTAT GTCCAAACAG 
GAGCTTGATG ATATCCTCAA ATTTGGCACT GAGGAACTAT TCAAGGATGA AGCCACTGAT 
GGAGGAGGAG ACAACAAAGA GGGAGAAGAT AGCAGTGTTA TCCACTACGA TGATAAGGCC 
ATTGAACGGC TGCTAGACCG TAACCAGGAT GAGACTGAAG ACACAGAATT GCAGGGCATG 
AATGAATATT TGAGCTCATT CAAAGTGGCC CAGTATGTGG TACGGGAAGA AGAAATGGGG 
GAGGAAGAGG AGGTAGAACG GGAAATCATT AAACAGGAAG AAAGTGTGGA TCCTGACTAC 
TGGGAGAAAT TGCTGCGGCA CCATTATGAG CAGCAGCAAG AAGATCTAGC CCGAAATCTG 
GGCAAAGGAA AAAGAATCCG TAAACAGGTC AACTACAATG ATGGCTCCCA GGAGGACCGA 
GATTGGCAGG ACGACCAGTC CGACAACCAG TCCGATTACT CAGTGGCTTC AGAGGAAGGT 
GATGAAGACT TTGATGAACG TTCAGAAGCT CCCCGTAGGC CCAGTCGTAA GGGCCTGCGG 
AATGATAAAG ATAAGCCATT GCCTCCTCTG TTGGCCCGTG TTGGTGGGAA TATTGAAGTA 
CTTGGTTTTA ATGCTCGTCA GCGAAAAGCC TTTCTTAATG CAATTATGCG ATATGGTATG 
CCACCTCAGG ATGCTTTTAC TACCCAGTGG CTTGTAAGAG ACCTGCGAGG CAAATCAGAG 
AAAGAGTTCA AGGCATATGT CTCTCTTTTC ATGCGGCATT TATGTGAGCC GGGGGCAGAT 
GGGGCTGAGA CCTTTGCTGA TGGTGTCCCC CGAGAAGGCC TGTCTCGCCA GCATGTCCTT 
ACTAGAATTG GTGTTATGTC TTTGATTCGC AAGAAGGTTC AGGAGTTTGA ACATGTTAAT 
GGGCGCTGGA GCATGCCTGA ACTGGCTGAG GTGGAGGAAA ACAAGAAGAT GTCCCAGCCA 
GGGTCACCCT CCCCAAAAAC TCCTACACCC TCCACTCCAG GGGACACGCA GCCCAACACT 
CCTGCACCTG TCCCACCTGC TGAAGATGGG ATAAAAATAG AGGAAAATAG CCTCAAAGAA 
GAAGAGAGCA TAGAAGGAGA AAAGGAGGTT AAATCTACAG CCCCTGAGAC TGCCATTGAG 
TGTACACAGG CCCCTGCCCC TGCCTCAGAG GATGAAAAGG TCGTTGTTGA ACCCCCTGAG 
GGAGAGGAGA AAGTGGAAAA GGCAGAGGTG AAGGAGAGAA CAGAGGAACC TATGGAGACA 
GAGCCCAAAG GTAAAGGTGC TGCTGATGTA GAGAAGGTGG AGGAAAAGTC AGCAATAGAT 
CTGACCCCTA TTGTGGTAGA AGACAAAGAA GAGAAGAAAG AAGAAGAAGA GAAAAAAGAG 
GTGATGCTTC AGAATGGAGA GACCCCCAAG GACCTGAATG ATGAGAAACA GAAGAAAAAT 
ATTAAACAAC GTTTCATGTT TAACATTGCA GATGGTGGTT TTACTGAGTT GCACTCCCTT 
TGGCAGAATG AAGAGCGGGC AGCCACAGTT ACCAAGAAGA CTTATGAGAT CTGGCATCGA 
CGGCATGACT ACTGGCTGCT AGCCGGCATT ATAAACCATG GCTATGCCCG GTGGCAAGAC 
ATCCAGAATG ACCCACGCTA TGCCATCCTC AATGAGCCTT TCAAGGGTGA AATGAACCGT 
GGCAATTTCT TAGAGATCAA GAATAAATTT CTAGCTCGAA GGTTTAAGCT CTTAGAACAA 
GCTCTGGTGA TTGAGGAACA GCTGCGCCGG GCTGCTTACT TGAACATGTC AGAAGACCCT 
TCTCACCCTT CCATGGCCCT CAACACCCGC TTTGCTGAGG TGGAGTGTTT GGCGGAAAGT 
CATCAGCACC TGTCCAAGGA GTCAATGGCA GGAAACAAGC CAGCCAATGC AGTCCTGCAC 
AAAGGTATTC TGAAACAGCT GGAAGAACTG CTGAGTGACA TGAAAGCTGA TGTGACTCGA 
CTCCCAGCTA CCATTGCCCG AATTCCCCCA GTTGCTGTGA GGTTACAGAT GTCAGAGCGT 
AACATTCTCA GCCGCCTGGC AAACCGGGCA CCCGAACCTA CCCCACAGCA GGTAGCCCAG 
CAGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHD4 ( XM_005253668.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005253725.1 Homo sapiens chromodomain helicase DNA binding protein 4 (CHD4), transcript variant X14, mRNA.

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CloneID	OHu28781
Clone ID Related Accession (Same CDS sequence)	NM_001297553.1 , NM_001297553.2 , XM_005253668.3
Accession Version	XM_005253668.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5718bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-25
Organism	Homo sapiens(human)
Product	chromodomain-helicase-DNA-binding protein 4 isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_005253668.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCGG GCCTGGGCTC CCCGTCCCCC TGCTCGGCGG GCAGTGAGGA GGAGGATATG 
GATGCACTTT TGAACAACAG CCTGCCCCCA CCCCACCCAG AAAATGAAGA GGACCCAGAA 
GAGGATTTGT CAGAAACAGA GACTCCAAAG CTCAAGAAGA AGAAAAAGCC TAAGAAACCT 
CGGGACCCTA AAATCCCTAA GAGCAAGCGC CAAAAAAAGG AGCTGGGGGA CAGCTCTGGG 
GAGGGGCCAG AGTTTGTGGA GGAGGAGGAA GAGGTGGCTC TGCGCTCAGA CAGTGAGGGC 
AGCGACTATA CTCCTGGCAA GAAGAAGAAG AAGAAGCTTG GACCTAAGAA AGAGAAGAAG 
AGCAAATCCA AGCGGAAGGA GGAGGAGGAG GAGGAGGATG ATGATGATGA TTCAAAGGAG 
CCTAAATCAT CTGCTCAGCT CCTGGAAGAC TGGGGCATGG AAGACATTGA CCACGTGTTC 
TCAGAGGAGG ATTATCGAAC CCTCACCAAC TACAAGGCCT TCAGCCAGTT TGTCAGACCC 
CTCATTGCTG CCAAAAATCC CAAGATTGCT GTCTCCAAGA TGATGATGGT TTTGGGTGCA 
AAATGGCGGG AGTTCAGTAC CAATAACCCC TTCAAAGGCA GTTCTGGGGC ATCAGTGGCA 
GCTGCGGCAG CAGCAGCGGT AGCTGTGGTG GAGAGCATGG TGACAGCCAC TGAGGTTGCA 
CCACCACCTC CCCCTGTGGA GGTGCCTATC CGCAAGGCCA AGACCAAGGA GGGCAAAGGT 
CCCAATGCTC GGAGGAAGCC CAAGGGCAGC CCTCGTGTAC CTGATGCCAA GAAGCCTAAA 
CCCAAGAAAG TAGCTCCCCT GAAAATCAAG CTGGGAGGTT TTGGTTCCAA GCGTAAGAGA 
TCCTCGAGTG AGGATGATGA CTTAGATGTG GAATCTGACT TCGATGATGC CAGTATCAAT 
AGCTATTCTG TTTCTGATGG TTCCACCAGC CGTAGTAGCC GCAGCCGCAA GAAACTCCGA 
ACCACTAAAA AGAAAAAGAA AGGCGAGGAG GAGGTGACTG CTGTGGATGG TTATGAGACA 
GACCACCAGG ACTATTGCGA GGTGTGCCAG CAAGGCGGTG AGATCATCCT GTGTGATACC 
TGTCCCCGTG CTTACCACAT GGTCTGCCTG GATCCCGACA TGGAGAAGGC TCCCGAGGGC 
AAGTGGAGCT GCCCACACTG CGAGAAGGAA GGCATCCAGT GGGAAGCTAA AGAGGACAAT 
TCGGAGGGTG AGGAGATCCT GGAAGAGGTT GGGGGAGACC TCGAAGAGGA GGATGACCAC 
CATATGGAAT TCTGTCGGGT CTGCAAGGAT GGTGGGGAAC TGCTCTGCTG TGATACCTGT 
CCTTCTTCCT ACCACATCCA CTGCCTGAAT CCCCCACTTC CAGAGATCCC CAACGGTGAA 
TGGCTCTGTC CCCGTTGTAC GTGTCCAGCT CTGAAGGGCA AAGTGCAGAA GATCCTAATC 
TGGAAGTGGG GTCAGCCACC ATCTCCCACA CCAGTGCCTC GGCCTCCAGA TGCTGATCCC 
AACACGCCCT CCCCAAAGCC CTTGGAGGGG CGGCCAGAGC GGCAGTTCTT TGTGAAATGG 
CAAGGCATGT CTTACTGGCA CTGCTCCTGG GTTTCTGAAC TGCAGCTGGA GCTGCACTGT 
CAGGTGATGT TCCGAAACTA TCAGCGGAAG AATGATATGG ATGAGCCACC TTCTGGGGAC 
TTTGGTGGTG ATGAAGAGAA AAGCCGAAAG CGAAAGAACA AGGACCCTAA ATTTGCAGAG 
ATGGAGGAAC GCTTCTATCG CTATGGGATA AAACCCGAGT GGATGATGAT CCACCGAATC 
CTCAACCACA GTGTGGACAA GAAGGGCCAC GTCCACTACT TGATCAAGTG GCGGGACTTA 
CCTTACGATC AGGCTTCTTG GGAGAGTGAG GATGTGGAGA TCCAGGATTA CGACCTGTTC 
AAGCAGAGCT ATTGGAATCA CAGGGAGTTA ATGAGGGGTG AGGAAGGCCG ACCAGGCAAG 
AAGCTCAAGA AGGTGAAGCT TCGGAAGTTG GAGAGGCCTC CAGAAACGCC AACAGTTGAT 
CCAACAGTGA AGTATGAGCG ACAGCCAGAG TACCTGGATG CTACAGGTGG AACCCTGCAC 
CCCTATCAAA TGGAGGGCCT GAATTGGTTG CGCTTCTCCT GGGCTCAGGG CACTGACACC 
ATCTTGGCTG ATGAGATGGG CCTTGGGAAA ACTGTACAGA CAGCAGTCTT CCTGTATTCC 
CTTTACAAGG AGGGTCATTC CAAAGGCCCC TTCCTAGTGA GCGCCCCTCT TTCTACCATC 
ATCAACTGGG AGCGGGAGTT TGAAATGTGG GCTCCAGACA TGTATGTCGT AACCTATGTG 
GGTGACAAGG ACAGCCGTGC CATCATCCGA GAGAATGAGT TCTCCTTTGA AGACAATGCC 
ATTCGTGGTG GCAAGAAGGC CTCCCGCATG AAGAAAGAGG CATCTGTGAA ATTCCATGTG 
CTGCTGACAT CCTATGAATT GATCACCATT GACATGGCTA TTTTGGGCTC TATTGATTGG 
GCCTGCCTCA TCGTGGATGA AGCCCATCGG CTGAAGAACA ATCAGTCTAA GTTCTTCCGG 
GTATTGAATG GTTACTCACT CCAGCACAAG CTGTTGCTGA CTGGGACACC ATTACAAAAC 
AATCTGGAAG AGTTGTTTCA TCTGCTCAAC TTTCTCACCC CCGAGAGGTT CCACAATTTG 
GAAGGTTTTT TGGAGGAGTT TGCTGACATT GCCAAGGAGG ACCAGATAAA AAAACTGCAT 
GACATGCTGG GGCCGCACAT GTTGCGGCGG CTCAAAGCCG ATGTGTTCAA GAACATGCCC 
TCCAAGACAG AACTAATTGT GCGTGTGGAG CTGAGCCCTA TGCAGAAGAA ATACTACAAG 
TACATCCTCA CTCGAAATTT TGAAGCACTC AATGCCCGAG GTGGTGGCAA CCAGGTGTCT 
CTGCTGAATG TGGTGATGGA TCTTAAGAAG TGCTGCAACC ATCCATACCT CTTCCCTGTG 
GCTGCAATGG AAGCTCCTAA GATGCCTAAT GGCATGTATG ATGGCAGTGC CCTAATCAGA 
GCATCTGGGA AATTATTGCT GCTGCAGAAA ATGCTCAAGA ACCTTAAGGA GGGTGGGCAT 
CGTGTACTCA TCTTTTCCCA GATGACCAAG ATGCTAGACC TGCTAGAGGA TTTCTTGGAA 
CATGAAGGTT ATAAATACGA ACGCATCGAT GGTGGAATCA CTGGGAACAT GCGGCAAGAG 
GCCATTGACC GCTTCAATGC ACCGGGTGCT CAGCAGTTCT GCTTCTTGCT TTCCACTCGA 
GCTGGGGGCC TTGGAATCAA TCTGGCCACT GCTGACACAG TTATTATCTA TGACTCTGAC 
TGGAACCCCC ATAATGACAT TCAGGCCTTT AGCAGAGCTC ACCGGATTGG GCAAAATAAA 
AAGGTAATGA TCTACCGGTT TGTGACCCGT GCGTCAGTGG AGGAGCGCAT CACGCAGGTG 
GCAAAGAAGA AAATGATGCT GACGCATCTA GTGGTGCGGC CTGGGCTGGG CTCCAAGACT 
GGATCTATGT CCAAACAGGA GCTTGATGAT ATCCTCAAAT TTGGCACTGA GGAACTATTC 
AAGGATGAAG CCACTGATGG AGGAGGAGAC AACAAAGAGG GAGAAGATAG CAGTGTTATC 
CACTACGATG ATAAGGCCAT TGAACGGCTG CTAGACCGTA ACCAGGATGA GACTGAAGAC 
ACAGAATTGC AGGGCATGAA TGAATATTTG AGCTCATTCA AAGTGGCCCA GTATGTGGTA 
CGGGAAGAAG AAATGGGGGA GGAAGAGGAG GTAGAACGGG AAATCATTAA ACAGGAAGAA 
AGTGTGGATC CTGACTACTG GGAGAAATTG CTGCGGCACC ATTATGAGCA GCAGCAAGAA 
GATCTAGCCC GAAATCTGGG CAAAGGAAAA AGAATCCGTA AACAGGTCAA CTACAATGAT 
GGCTCCCAGG AGGACCGAGA TTGGCAGGAC GACCAGTCCG ACAACCAGTC CGATTACTCA 
GTGGCTTCAG AGGAAGGTGA TGAAGACTTT GATGAACGTT CAGAAGCTCC CCGTAGGCCC 
AGTCGTAAGG GCCTGCGGAA TGATAAAGAT AAGCCATTGC CTCCTCTGTT GGCCCGTGTT 
GGTGGGAATA TTGAAGTACT TGGTTTTAAT GCTCGTCAGC GAAAAGCCTT TCTTAATGCA 
ATTATGCGAT ATGGTATGCC ACCTCAGGAT GCTTTTACTA CCCAGTGGCT TGTAAGAGAC 
CTGCGAGGCA AATCAGAGAA AGAGTTCAAG GCATATGTCT CTCTTTTCAT GCGGCATTTA 
TGTGAGCCGG GGGCAGATGG GGCTGAGACC TTTGCTGATG GTGTCCCCCG AGAAGGCCTG 
TCTCGCCAGC ATGTCCTTAC TAGAATTGGT GTTATGTCTT TGATTCGCAA GAAGGTTCAG 
GAGTTTGAAC ATGTTAATGG GCGCTGGAGC ATGCCTGAAC TGGCTGAGGT GGAGGAAAAC 
AAGAAGATGT CCCAGCCAGG GTCACCCTCC CCAAAAACTC CTACACCCTC CACTCCAGGG 
GACACGCAGC CCAACACTCC TGCACCTGTC CCACCTGCTG AAGATGGGAT AAAAATAGAG 
GAAAATAGCC TCAAAGAAGA AGAGAGCATA GAAGGAGAAA AGGAGGTTAA ATCTACAGCC 
CCTGAGACTG CCATTGAGTG TACACAGGCC CCTGCCCCTG CCTCAGAGGA TGAAAAGGTC 
GTTGTTGAAC CCCCTGAGGG AGAGGAGAAA GTGGAAAAGG CAGAGGTGAA GGAGAGAACA 
GAGGAACCTA TGGAGACAGA GCCCAAAGGT GCTGCTGATG TAGAGAAGGT GGAGGAAAAG 
TCAGCAATAG ATCTGACCCC TATTGTGGTA GAAGACAAAG AAGAGAAGAA AGAAGAAGAA 
GAGAAAAAAG AGGTGATGCT TCAGAATGGA GAGACCCCCA AGGACCTGAA TGATGAGAAA 
CAGAAGAAAA ATATTAAACA ACGTTTCATG TTTAACATTG CAGATGGTGG TTTTACTGAG 
TTGCACTCCC TTTGGCAGAA TGAAGAGCGG GCAGCCACAG TTACCAAGAA GACTTATGAG 
ATCTGGCATC GACGGCATGA CTACTGGCTG CTAGCCGGCA TTATAAACCA TGGCTATGCC 
CGGTGGCAAG ACATCCAGAA TGACCCACGC TATGCCATCC TCAATGAGCC TTTCAAGGGT 
GAAATGAACC GTGGCAATTT CTTAGAGATC AAGAATAAAT TTCTAGCTCG AAGGTTTAAG 
CTCTTAGAAC AAGCTCTGGT GATTGAGGAA CAGCTGCGCC GGGCTGCTTA CTTGAACATG 
TCAGAAGACC CTTCTCACCC TTCCATGGCC CTCAACACCC GCTTTGCTGA GGTGGAGTGT 
TTGGCGGAAA GTCATCAGCA CCTGTCCAAG GAGTCAATGG CAGGAAACAA GCCAGCCAAT 
GCAGTCCTGC ACAAAGTTCT GAAACAGCTG GAAGAACTGC TGAGTGACAT GAAAGCTGAT 
GTGACTCGAC TCCCAGCTAC CATTGCCCGA ATTCCCCCAG TTGCTGTGAG GTTACAGATG 
TCAGAGCGTA ACATTCTCAG CCGCCTGGCA AACCGGGCAC CCGAACCTAC CCCACAGCAG 
GTAGCCCAGC AGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005253725.1
CDS	165..5882
Translation	MASGLGSPSPCSAGSEEEDMDALLNNSLPPPHPENEEDPEEDLSETETPKLKKKKKPKKPRDPKIPKSKRQKKELGDSSGEGPEFVEEEEEVALRSDSEGSDYTPGKKKKKKLGPKKEKKSKSKRKEEEEEEDDDDDSKEPKSSAQLLEDWGMEDIDHVFSEEDYRTLTNYKAFSQFVRPLIAAKNPKIAVSKMMMVLGAKWREFSTNNPFKGSSGASVAAAAAAAVAVVESMVTATEVAPPPPPVEVPIRKAKTKEGKGPNARRKPKGSPRVPDAKKPKPKKVAPLKIKLGGFGSKRKRSSSEDDDLDVESDFDDASINSYSVSDGSTSRSSRSRKKLRTTKKKKKGEEEVTAVDGYETDHQDYCEVCQQGGEIILCDTCPRAYHMVCLDPDMEKAPEGKWSCPHCEKEGIQWEAKEDNSEGEEILEEVGGDLEEEDDHHMEFCRVCKDGGELLCCDTCPSSYHIHCLNPPLPEIPNGEWLCPRCTCPALKGKVQKILIWKWGQPPSPTPVPRPPDADPNTPSPKPLEGRPERQFFVKWQGMSYWHCSWVSELQLELHCQVMFRNYQRKNDMDEPPSGDFGGDEEKSRKRKNKDPKFAEMEERFYRYGIKPEWMMIHRILNHSVDKKGHVHYLIKWRDLPYDQASWESEDVEIQDYDLFKQSYWNHRELMRGEEGRPGKKLKKVKLRKLERPPETPTVDPTVKYERQPEYLDATGGTLHPYQMEGLNWLRFSWAQGTDTILADEMGLGKTVQTAVFLYSLYKEGHSKGPFLVSAPLSTIINWEREFEMWAPDMYVVTYVGDKDSRAIIRENEFSFEDNAIRGGKKASRMKKEASVKFHVLLTSYELITIDMAILGSIDWACLIVDEAHRLKNNQSKFFRVLNGYSLQHKLLLTGTPLQNNLEELFHLLNFLTPERFHNLEGFLEEFADIAKEDQIKKLHDMLGPHMLRRLKADVFKNMPSKTELIVRVELSPMQKKYYKYILTRNFEALNARGGGNQVSLLNVVMDLKKCCNHPYLFPVAAMEAPKMPNGMYDGSALIRASGKLLLLQKMLKNLKEGGHRVLIFSQMTKMLDLLEDFLEHEGYKYERIDGGITGNMRQEAIDRFNAPGAQQFCFLLSTRAGGLGINLATADTVIIYDSDWNPHNDIQAFSRAHRIGQNKKVMIYRFVTRASVEERITQVAKKKMMLTHLVVRPGLGSKTGSMSKQELDDILKFGTEELFKDEATDGGGDNKEGEDSSVIHYDDKAIERLLDRNQDETEDTELQGMNEYLSSFKVAQYVVREEEMGEEEEVEREIIKQEESVDPDYWEKLLRHHYEQQQEDLARNLGKGKRIRKQVNYNDGSQEDRDWQDDQSDNQSDYSVASEEGDEDFDERSEAPRRPSRKGLRNDKDKPLPPLLARVGGNIEVLGFNARQRKAFLNAIMRYGMPPQDAFTTQWLVRDLRGKSEKEFKAYVSLFMRHLCEPGADGAETFADGVPREGLSRQHVLTRIGVMSLIRKKVQEFEHVNGRWSMPELAEVEENKKMSQPGSPSPKTPTPSTPGDTQPNTPAPVPPAEDGIKIEENSLKEEESIEGEKEVKSTAPETAIECTQAPAPASEDEKVVVEPPEGEEKVEKAEVKERTEEPMETEPKGAADVEKVEEKSAIDLTPIVVEDKEEKKEEEEKKEVMLQNGETPKDLNDEKQKKNIKQRFMFNIADGGFTELHSLWQNEERAATVTKKTYEIWHRRHDYWLLAGIINHGYARWQDIQNDPRYAILNEPFKGEMNRGNFLEIKNKFLARRFKLLEQALVIEEQLRRAAYLNMSEDPSHPSMALNTRFAEVECLAESHQHLSKESMAGNKPANAVLHKVLKQLEELLSDMKADVTRLPATIARIPPVAVRLQMSERNILSRLANRAPEPTPQQVAQQQ

Target ORF information:

RefSeq Version	XM_005253668.3
Organism	Homo sapiens(human)
Definition	Homo sapiens chromodomain helicase DNA binding protein 4 (CHD4), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005253668.3

ORF Insert Sequence:

ATGGCGTCGG GCCTGGGCTC CCCGTCCCCC TGCTCGGCGG GCAGTGAGGA GGAGGATATG 
GATGCACTTT TGAACAACAG CCTGCCCCCA CCCCACCCAG AAAATGAAGA GGACCCAGAA 
GAGGATTTGT CAGAAACAGA GACTCCAAAG CTCAAGAAGA AGAAAAAGCC TAAGAAACCT 
CGGGACCCTA AAATCCCTAA GAGCAAGCGC CAAAAAAAGG AGCTGGGGGA CAGCTCTGGG 
GAGGGGCCAG AGTTTGTGGA GGAGGAGGAA GAGGTGGCTC TGCGCTCAGA CAGTGAGGGC 
AGCGACTATA CTCCTGGCAA GAAGAAGAAG AAGAAGCTTG GACCTAAGAA AGAGAAGAAG 
AGCAAATCCA AGCGGAAGGA GGAGGAGGAG GAGGAGGATG ATGATGATGA TTCAAAGGAG 
CCTAAATCAT CTGCTCAGCT CCTGGAAGAC TGGGGCATGG AAGACATTGA CCACGTGTTC 
TCAGAGGAGG ATTATCGAAC CCTCACCAAC TACAAGGCCT TCAGCCAGTT TGTCAGACCC 
CTCATTGCTG CCAAAAATCC CAAGATTGCT GTCTCCAAGA TGATGATGGT TTTGGGTGCA 
AAATGGCGGG AGTTCAGTAC CAATAACCCC TTCAAAGGCA GTTCTGGGGC ATCAGTGGCA 
GCTGCGGCAG CAGCAGCGGT AGCTGTGGTG GAGAGCATGG TGACAGCCAC TGAGGTTGCA 
CCACCACCTC CCCCTGTGGA GGTGCCTATC CGCAAGGCCA AGACCAAGGA GGGCAAAGGT 
CCCAATGCTC GGAGGAAGCC CAAGGGCAGC CCTCGTGTAC CTGATGCCAA GAAGCCTAAA 
CCCAAGAAAG TAGCTCCCCT GAAAATCAAG CTGGGAGGTT TTGGTTCCAA GCGTAAGAGA 
TCCTCGAGTG AGGATGATGA CTTAGATGTG GAATCTGACT TCGATGATGC CAGTATCAAT 
AGCTATTCTG TTTCTGATGG TTCCACCAGC CGTAGTAGCC GCAGCCGCAA GAAACTCCGA 
ACCACTAAAA AGAAAAAGAA AGGCGAGGAG GAGGTGACTG CTGTGGATGG TTATGAGACA 
GACCACCAGG ACTATTGCGA GGTGTGCCAG CAAGGCGGTG AGATCATCCT GTGTGATACC 
TGTCCCCGTG CTTACCACAT GGTCTGCCTG GATCCCGACA TGGAGAAGGC TCCCGAGGGC 
AAGTGGAGCT GCCCACACTG CGAGAAGGAA GGCATCCAGT GGGAAGCTAA AGAGGACAAT 
TCGGAGGGTG AGGAGATCCT GGAAGAGGTT GGGGGAGACC TCGAAGAGGA GGATGACCAC 
CATATGGAAT TCTGTCGGGT CTGCAAGGAT GGTGGGGAAC TGCTCTGCTG TGATACCTGT 
CCTTCTTCCT ACCACATCCA CTGCCTGAAT CCCCCACTTC CAGAGATCCC CAACGGTGAA 
TGGCTCTGTC CCCGTTGTAC GTGTCCAGCT CTGAAGGGCA AAGTGCAGAA GATCCTAATC 
TGGAAGTGGG GTCAGCCACC ATCTCCCACA CCAGTGCCTC GGCCTCCAGA TGCTGATCCC 
AACACGCCCT CCCCAAAGCC CTTGGAGGGG CGGCCAGAGC GGCAGTTCTT TGTGAAATGG 
CAAGGCATGT CTTACTGGCA CTGCTCCTGG GTTTCTGAAC TGCAGCTGGA GCTGCACTGT 
CAGGTGATGT TCCGAAACTA TCAGCGGAAG AATGATATGG ATGAGCCACC TTCTGGGGAC 
TTTGGTGGTG ATGAAGAGAA AAGCCGAAAG CGAAAGAACA AGGACCCTAA ATTTGCAGAG 
ATGGAGGAAC GCTTCTATCG CTATGGGATA AAACCCGAGT GGATGATGAT CCACCGAATC 
CTCAACCACA GTGTGGACAA GAAGGGCCAC GTCCACTACT TGATCAAGTG GCGGGACTTA 
CCTTACGATC AGGCTTCTTG GGAGAGTGAG GATGTGGAGA TCCAGGATTA CGACCTGTTC 
AAGCAGAGCT ATTGGAATCA CAGGGAGTTA ATGAGGGGTG AGGAAGGCCG ACCAGGCAAG 
AAGCTCAAGA AGGTGAAGCT TCGGAAGTTG GAGAGGCCTC CAGAAACGCC AACAGTTGAT 
CCAACAGTGA AGTATGAGCG ACAGCCAGAG TACCTGGATG CTACAGGTGG AACCCTGCAC 
CCCTATCAAA TGGAGGGCCT GAATTGGTTG CGCTTCTCCT GGGCTCAGGG CACTGACACC 
ATCTTGGCTG ATGAGATGGG CCTTGGGAAA ACTGTACAGA CAGCAGTCTT CCTGTATTCC 
CTTTACAAGG AGGGTCATTC CAAAGGCCCC TTCCTAGTGA GCGCCCCTCT TTCTACCATC 
ATCAACTGGG AGCGGGAGTT TGAAATGTGG GCTCCAGACA TGTATGTCGT AACCTATGTG 
GGTGACAAGG ACAGCCGTGC CATCATCCGA GAGAATGAGT TCTCCTTTGA AGACAATGCC 
ATTCGTGGTG GCAAGAAGGC CTCCCGCATG AAGAAAGAGG CATCTGTGAA ATTCCATGTG 
CTGCTGACAT CCTATGAATT GATCACCATT GACATGGCTA TTTTGGGCTC TATTGATTGG 
GCCTGCCTCA TCGTGGATGA AGCCCATCGG CTGAAGAACA ATCAGTCTAA GTTCTTCCGG 
GTATTGAATG GTTACTCACT CCAGCACAAG CTGTTGCTGA CTGGGACACC ATTACAAAAC 
AATCTGGAAG AGTTGTTTCA TCTGCTCAAC TTTCTCACCC CCGAGAGGTT CCACAATTTG 
GAAGGTTTTT TGGAGGAGTT TGCTGACATT GCCAAGGAGG ACCAGATAAA AAAACTGCAT 
GACATGCTGG GGCCGCACAT GTTGCGGCGG CTCAAAGCCG ATGTGTTCAA GAACATGCCC 
TCCAAGACAG AACTAATTGT GCGTGTGGAG CTGAGCCCTA TGCAGAAGAA ATACTACAAG 
TACATCCTCA CTCGAAATTT TGAAGCACTC AATGCCCGAG GTGGTGGCAA CCAGGTGTCT 
CTGCTGAATG TGGTGATGGA TCTTAAGAAG TGCTGCAACC ATCCATACCT CTTCCCTGTG 
GCTGCAATGG AAGCTCCTAA GATGCCTAAT GGCATGTATG ATGGCAGTGC CCTAATCAGA 
GCATCTGGGA AATTATTGCT GCTGCAGAAA ATGCTCAAGA ACCTTAAGGA GGGTGGGCAT 
CGTGTACTCA TCTTTTCCCA GATGACCAAG ATGCTAGACC TGCTAGAGGA TTTCTTGGAA 
CATGAAGGTT ATAAATACGA ACGCATCGAT GGTGGAATCA CTGGGAACAT GCGGCAAGAG 
GCCATTGACC GCTTCAATGC ACCGGGTGCT CAGCAGTTCT GCTTCTTGCT TTCCACTCGA 
GCTGGGGGCC TTGGAATCAA TCTGGCCACT GCTGACACAG TTATTATCTA TGACTCTGAC 
TGGAACCCCC ATAATGACAT TCAGGCCTTT AGCAGAGCTC ACCGGATTGG GCAAAATAAA 
AAGGTAATGA TCTACCGGTT TGTGACCCGT GCGTCAGTGG AGGAGCGCAT CACGCAGGTG 
GCAAAGAAGA AAATGATGCT GACGCATCTA GTGGTGCGGC CTGGGCTGGG CTCCAAGACT 
GGATCTATGT CCAAACAGGA GCTTGATGAT ATCCTCAAAT TTGGCACTGA GGAACTATTC 
AAGGATGAAG CCACTGATGG AGGAGGAGAC AACAAAGAGG GAGAAGATAG CAGTGTTATC 
CACTACGATG ATAAGGCCAT TGAACGGCTG CTAGACCGTA ACCAGGATGA GACTGAAGAC 
ACAGAATTGC AGGGCATGAA TGAATATTTG AGCTCATTCA AAGTGGCCCA GTATGTGGTA 
CGGGAAGAAG AAATGGGGGA GGAAGAGGAG GTAGAACGGG AAATCATTAA ACAGGAAGAA 
AGTGTGGATC CTGACTACTG GGAGAAATTG CTGCGGCACC ATTATGAGCA GCAGCAAGAA 
GATCTAGCCC GAAATCTGGG CAAAGGAAAA AGAATCCGTA AACAGGTCAA CTACAATGAT 
GGCTCCCAGG AGGACCGAGA TTGGCAGGAC GACCAGTCCG ACAACCAGTC CGATTACTCA 
GTGGCTTCAG AGGAAGGTGA TGAAGACTTT GATGAACGTT CAGAAGCTCC CCGTAGGCCC 
AGTCGTAAGG GCCTGCGGAA TGATAAAGAT AAGCCATTGC CTCCTCTGTT GGCCCGTGTT 
GGTGGGAATA TTGAAGTACT TGGTTTTAAT GCTCGTCAGC GAAAAGCCTT TCTTAATGCA 
ATTATGCGAT ATGGTATGCC ACCTCAGGAT GCTTTTACTA CCCAGTGGCT TGTAAGAGAC 
CTGCGAGGCA AATCAGAGAA AGAGTTCAAG GCATATGTCT CTCTTTTCAT GCGGCATTTA 
TGTGAGCCGG GGGCAGATGG GGCTGAGACC TTTGCTGATG GTGTCCCCCG AGAAGGCCTG 
TCTCGCCAGC ATGTCCTTAC TAGAATTGGT GTTATGTCTT TGATTCGCAA GAAGGTTCAG 
GAGTTTGAAC ATGTTAATGG GCGCTGGAGC ATGCCTGAAC TGGCTGAGGT GGAGGAAAAC 
AAGAAGATGT CCCAGCCAGG GTCACCCTCC CCAAAAACTC CTACACCCTC CACTCCAGGG 
GACACGCAGC CCAACACTCC TGCACCTGTC CCACCTGCTG AAGATGGGAT AAAAATAGAG 
GAAAATAGCC TCAAAGAAGA AGAGAGCATA GAAGGAGAAA AGGAGGTTAA ATCTACAGCC 
CCTGAGACTG CCATTGAGTG TACACAGGCC CCTGCCCCTG CCTCAGAGGA TGAAAAGGTC 
GTTGTTGAAC CCCCTGAGGG AGAGGAGAAA GTGGAAAAGG CAGAGGTGAA GGAGAGAACA 
GAGGAACCTA TGGAGACAGA GCCCAAAGGT GCTGCTGATG TAGAGAAGGT GGAGGAAAAG 
TCAGCAATAG ATCTGACCCC TATTGTGGTA GAAGACAAAG AAGAGAAGAA AGAAGAAGAA 
GAGAAAAAAG AGGTGATGCT TCAGAATGGA GAGACCCCCA AGGACCTGAA TGATGAGAAA 
CAGAAGAAAA ATATTAAACA ACGTTTCATG TTTAACATTG CAGATGGTGG TTTTACTGAG 
TTGCACTCCC TTTGGCAGAA TGAAGAGCGG GCAGCCACAG TTACCAAGAA GACTTATGAG 
ATCTGGCATC GACGGCATGA CTACTGGCTG CTAGCCGGCA TTATAAACCA TGGCTATGCC 
CGGTGGCAAG ACATCCAGAA TGACCCACGC TATGCCATCC TCAATGAGCC TTTCAAGGGT 
GAAATGAACC GTGGCAATTT CTTAGAGATC AAGAATAAAT TTCTAGCTCG AAGGTTTAAG 
CTCTTAGAAC AAGCTCTGGT GATTGAGGAA CAGCTGCGCC GGGCTGCTTA CTTGAACATG 
TCAGAAGACC CTTCTCACCC TTCCATGGCC CTCAACACCC GCTTTGCTGA GGTGGAGTGT 
TTGGCGGAAA GTCATCAGCA CCTGTCCAAG GAGTCAATGG CAGGAAACAA GCCAGCCAAT 
GCAGTCCTGC ACAAAGTTCT GAAACAGCTG GAAGAACTGC TGAGTGACAT GAAAGCTGAT 
GTGACTCGAC TCCCAGCTAC CATTGCCCGA ATTCCCCCAG TTGCTGTGAG GTTACAGATG 
TCAGAGCGTA ACATTCTCAG CCGCCTGGCA AACCGGGCAC CCGAACCTAC CCCACAGCAG 
GTAGCCCAGC AGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHD4 ( XM_006718958.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_006719021.1 Homo sapiens chromodomain helicase DNA binding protein 4 (CHD4), transcript variant X2, mRNA.

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CloneID	OHu45950
Clone ID Related Accession (Same CDS sequence)	XM_006718958.1
Accession Version	XM_006718958.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5823bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-25
Organism	Homo sapiens(human)
Product	chromodomain-helicase-DNA-binding protein 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCGG GCCTGGGCTC CCCGTCCCCC TGCTCGGCGG GCAGTGAGGA GGAGGATATG 
GATGCACTTT TGAACAACAG CCTGCCCCCA CCCCACCCAG AAAATGAAGA GGACCCAGAA 
GAGGATTTGT CAGAAACAGA GACTCCAAAG CTCAAGAAGA AGAAAAAGCC TAAGAAACCT 
CGGGACCCTA AAATCCCTAA GAGCAAGCGC CAAAAAAAGG AGCGTATGCT CTTATGCCGG 
CAGCTGGGGG ACAGCTCTGG GGAGGGGCCA GAGTTTGTGG AGGAGGAGGA AGAGGTGGCT 
CTGCGCTCAG ACAGTGAGGG CAGCGACTAT ACTCCTGGCA AGAAGAAGAA GAAGAAGCTT 
GGACCTAAGA AAGAGAAGAA GAGCAAATCC AAGCGGAAGG AGGAGGAGGA GGAGGAGGAT 
GATGATGATG ATTCAAAGGA GCCTAAATCA TCTGCTCAGC TCCTGGAAGA CTGGGGCATG 
GAAGACATTG ACCACGTGTT CTCAGAGGAG GATTATCGAA CCCTCACCAA CTACAAGGCC 
TTCAGCCAGT TTGTCAGACC CCTCATTGCT GCCAAAAATC CCAAGATTGC TGTCTCCAAG 
ATGATGATGG TTTTGGGTGC AAAATGGCGG GAGTTCAGTA CCAATAACCC CTTCAAAGGC 
AGTTCTGGGG CATCAGTGGC AGCTGCGGCA GCAGCAGCGG TAGCTGTGGT GGAGAGCATG 
GTGACAGCCA CTGAGGTTGC ACCACCACCT CCCCCTGTGG AGGTGCCTAT CCGCAAGGCC 
AAGACCAAGG AGGGCAAAGG TCCCAATGCT CGGAGGAAGC CCAAGGGCAG CCCTCGTGTA 
CCTGATGCCA AGAAGCCTAA ACCCAAGAAA GTAGCTCCCC TGAAAATCAA GCTGGGAGGT 
TTTGGTTCCA AGCGTAAGAG ATCCTCGAGT GAGGATGATG ACTTAGATGT GGAATCTGAC 
TTCGATGATG CCAGTATCAA TAGCTATTCT GTTTCTGATG GTTCCACCAG CCGTAGTAGC 
CGCAGCCGCA AGAAACTCCG AACCACTAAA AAGAAAAAGA AAGGCGAGGA GGAGGTGACT 
GCTGTGGATG GTTATGAGAC AGACCACCAG GACTATTGCG AGGTGTGCCA GCAAGGCGGT 
GAGATCATCC TGTGTGATAC CTGTCCCCGT GCTTACCACA TGGTCTGCCT GGATCCCGAC 
ATGGAGAAGG CTCCCGAGGG CAAGTGGAGC TGCCCACACT GCGAGAAGGA AGGCATCCAG 
TGGGAAGCTA AAGAGGACAA TTCGGAGGGT GAGGAGATCC TGGAAGAGGT TGGGGGAGAC 
CTCGAAGAGG AGGATGACCA CCATATGGAA TTCTGTCGGG TCTGCAAGGA TGGTGGGGAA 
CTGCTCTGCT GTGATACCTG TCCTTCTTCC TACCACATCC ACTGCCTGAA TCCCCCACTT 
CCAGAGATCC CCAACGGTGA ATGGCTCTGT CCCCGTTGTA CGTGTCCAGC TCTGAAGGGC 
AAAGTGCAGA AGATCCTAAT CTGGAAGTGG GGTCAGCCAC CATCTCCCAC ACCAGTGCCT 
CGGCCTCCAG ATGCTGATCC CAACACGCCC TCCCCAAAGC CCTTGGAGGG GCGGCCAGAG 
CGGCAGTTCT TTGTGAAATG GCAAGGCATG TCTTACTGGC ACTGCTCCTG GGTTTCTGAA 
CTGCAGCTGG AGCTGCACTG TCAGGTGATG TTCCGAAACT ATCAGCGGAA GAATGATATG 
GATGAGCCAC CTTCTGGGGA CTTTGGTGGT GATGAAGAGA AAAGCCGAAA GCGAAAGAAC 
AAGGACCCTA AATTTGCAGA GATGGAGGAA CGCTTCTATC GCTATGGGAT AAAACCCGAG 
TGGATGATGA TCCACCGAAT CCTCAACCAC AGTGTGGACA AGAAGGGCCA CGTCCACTAC 
TTGATCAAGT GGCGGGACTT ACCTTACGAT CAGGCTTCTT GGGAGAGTGA GGATGTGGAG 
ATCCAGGATT ACGACCTGTT CAAGCAGAGC TATTGGAATC ACAGGGAGTT AATGAGGGGT 
GAGGAAGGCC GACCAGGCAA GAAGCTCAAG AAGGTGAAGC TTCGGAAGTT GGAGAGGCCT 
CCAGAAACGC CAACAGTTGA TCCAACAGTG AAGTATGAGC GACAGCCAGA GTACCTGGAT 
GCTACAGGTG GAACCCTGCA CCCCTATCAA ATGGAGGGCC TGAATTGGTT GCGCTTCTCC 
TGGGCTCAGG GCACTGACAC CATCTTGGCT GATGAGATGG GCCTTGGGAA AACTGTACAG 
ACAGCAGTCT TCCTGTATTC CCTTTACAAG GAGGGTCATT CCAAAGGCCC CTTCCTAGTG 
AGCGCCCCTC TTTCTACCAT CATCAACTGG GAGCGGGAGT TTGAAATGTG GGCTCCAGAC 
ATGTATGTCG TAACCTATGT GGGTGACAAG GACAGCCGTG CCATCATCCG AGAGAATGAG 
TTCTCCTTTG AAGACAATGC CATTCGTGGT GGCAAGAAGG CCTCCCGCAT GAAGAAAGAG 
GCATCTGTGA AATTCCATGT GCTGCTGACA TCCTATGAAT TGATCACCAT TGACATGGCT 
ATTTTGGGCT CTATTGATTG GGCCTGCCTC ATCGTGGATG AAGCCCATCG GCTGAAGAAC 
AATCAGTCTA AGTTCTTCCG GGTATTGAAT GGTTACTCAC TCCAGCACAA GCTGTTGCTG 
ACTGGGACAC CATTACAAAA CAATCTGGAA GAGTTGTTTC ATCTGCTCAA CTTTCTCACC 
CCCGAGAGGT TCCACAATTT GGAAGGTTTT TTGGAGGAGT TTGCTGACAT TGCCAAGGAG 
GACCAGATAA AAAAACTGCA TGACATGCTG GGGCCGCACA TGTTGCGGCG GCTCAAAGCC 
GATGTGTTCA AGAACATGCC CTCCAAGACA GAACTAATTG TGCGTGTGGA GCTGAGCCCT 
ATGCAGAAGA AATACTACAA GTACATCCTC ACTCGAAATT TTGAAGCACT CAATGCCCGA 
GGTGGTGGCA ACCAGGTGTC TCTGCTGAAT GTGGTGATGG ATCTTAAGAA GTGCTGCAAC 
CATCCATACC TCTTCCCTGT GGCTGCAATG GAAGCTCCTA AGATGCCTAA TGGCATGTAT 
GATGGCAGTG CCCTAATCAG AGCATCTGGG AAATTATTGC TGCTGCAGAA AATGCTCAAG 
AACCTTAAGG AGGGTGGGCA TCGTGTACTC ATCTTTTCCC AGATGACCAA GATGCTAGAC 
CTGCTAGAGG ATTTCTTGGA ACATGAAGGT TATAAATACG AACGCATCGA TGGTGGAATC 
ACTGGGAACA TGCGGCAAGA GGCCATTGAC CGCTTCAATG CACCGGGTGC TCAGCAGTTC 
TGCTTCTTGC TTTCCACTCG AGCTGGGGGC CTTGGAATCA ATCTGGCCAC TGCTGACACA 
GTTATTATCT ATGACTCTGA CTGGAACCCC CATAATGACA TTCAGGCCTT TAGCAGAGCT 
CACCGGATTG GGCAAAATAA AAAGGTAATG ATCTACCGGT TTGTGACCCG TGCGTCAGTG 
GAGGAGCGCA TCACGCAGGT GGCAAAGAAG AAAATGATGC TGACGCATCT AGTGGTGCGG 
CCTGGGCTGG GCTCCAAGAC TGGATCTATG TCCAAACAGG AGCTTGATGA TATCCTCAAA 
TTTGGCACTG AGGAACTATT CAAGGATGAA GCCACTGATG GAGGAGGAGA CAACAAAGAG 
GGAGAAGATA GCAGTGTTAT CCACTACGAT GATAAGGCCA TTGAACGGCT GCTAGACCGT 
AACCAGGATG AGACTGAAGA CACAGAATTG CAGGGCATGA ATGAATATTT GAGCTCATTC 
AAAGTGGCCC AGTATGTGGT ACGGGAAGAA GAAATGGGGG AGGAAGAGGA GGTAGAACGG 
GAAATCATTA AACAGGAAGA AAGTGTGGAT CCTGACTACT GGGAGAAATT GCTGCGGCAC 
CATTATGAGC AGCAGCAAGA AGATCTAGCC CGAAATCTGG GCAAAGGAAA AAGAATCCGT 
AAACAGGTCA ACTACAATGA TGGCTCCCAG GAGGACCGAG GTGTGTGTGG CCGGCCCCGC 
CCCCCACCCA TGGGCCGTTC CACTAGAGCA GTGGGCCCCG CTCATCTGCC CTCTCTCCCT 
CCAGATTGGC AGGACGACCA GTCCGACAAC CAGTCCGATT ACTCAGTGGC TTCAGAGGAA 
GGTGATGAAG ACTTTGATGA ACGTTCAGAA GCTCCCCGTA GGCCCAGTCG TAAGGGCCTG 
CGGAATGATA AAGATAAGCC ATTGCCTCCT CTGTTGGCCC GTGTTGGTGG GAATATTGAA 
GTACTTGGTT TTAATGCTCG TCAGCGAAAA GCCTTTCTTA ATGCAATTAT GCGATATGGT 
ATGCCACCTC AGGATGCTTT TACTACCCAG TGGCTTGTAA GAGACCTGCG AGGCAAATCA 
GAGAAAGAGT TCAAGGCATA TGTCTCTCTT TTCATGCGGC ATTTATGTGA GCCGGGGGCA 
GATGGGGCTG AGACCTTTGC TGATGGTGTC CCCCGAGAAG GCCTGTCTCG CCAGCATGTC 
CTTACTAGAA TTGGTGTTAT GTCTTTGATT CGCAAGAAGG TTCAGGAGTT TGAACATGTT 
AATGGGCGCT GGAGCATGCC TGAACTGGCT GAGGTGGAGG AAAACAAGAA GATGTCCCAG 
CCAGGGTCAC CCTCCCCAAA AACTCCTACA CCCTCCACTC CAGGGGACAC GCAGCCCAAC 
ACTCCTGCAC CTGTCCCACC TGCTGAAGAT GGGATAAAAA TAGAGGAAAA TAGCCTCAAA 
GAAGAAGAGA GCATAGAAGG AGAAAAGGAG GTTAAATCTA CAGCCCCTGA GACTGCCATT 
GAGTGTACAC AGGCCCCTGC CCCTGCCTCA GAGGATGAAA AGGTCGTTGT TGAACCCCCT 
GAGGGAGAGG AGAAAGTGGA AAAGGCAGAG GTGAAGGAGA GAACAGAGGA ACCTATGGAG 
ACAGAGCCCA AAGGTGCTGC TGATGTAGAG AAGGTGGAGG AAAAGTCAGC AATAGATCTG 
ACCCCTATTG TGGTAGAAGA CAAAGAAGAG AAGAAAGAAG AAGAAGAGAA AAAAGAGGTG 
ATGCTTCAGA ATGGAGAGAC CCCCAAGGAC CTGAATGATG AGAAACAGAA GAAAAATATT 
AAACAACGTT TCATGTTTAA CATTGCAGAT GGTGGTTTTA CTGAGTTGCA CTCCCTTTGG 
CAGAATGAAG AGCGGGCAGC CACAGTTACC AAGAAGACTT ATGAGATCTG GCATCGACGG 
CATGACTACT GGCTGCTAGC CGGCATTATA AACCATGGCT ATGCCCGGTG GCAAGACATC 
CAGAATGACC CACGCTATGC CATCCTCAAT GAGCCTTTCA AGGGTGAAAT GAACCGTGGC 
AATTTCTTAG AGATCAAGAA TAAATTTCTA GCTCGAAGGT TTAAGCTCTT AGAACAAGCT 
CTGGTGATTG AGGAACAGCT GCGCCGGGCT GCTTACTTGA ACATGTCAGA AGACCCTTCT 
CACCCTTCCA TGGCCCTCAA CACCCGCTTT GCTGAGGTGG AGTGTTTGGC GGAAAGTCAT 
CAGCACCTGT CCAAGGAGTC AATGGCAGGA AACAAGCCAG CCAATGCAGT CCTGCACAAA 
GTTCTGAAAC AGCTGGAAGA ACTGCTGAGT GACATGAAAG CTGATGTGAC TCGACTCCCA 
GCTACCATTG CCCGAATTCC CCCAGTTGCT GTGAGGTTAC AGATGTCAGA GCGTAACATT 
CTCAGCCGCC TGGCAAACCG GGCACCCGAA CCTACCCCAC AGCAGGTAGC CCAGCAGCAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006719021.1
CDS	219..6041
Translation	MASGLGSPSPCSAGSEEEDMDALLNNSLPPPHPENEEDPEEDLSETETPKLKKKKKPKKPRDPKIPKSKRQKKERMLLCRQLGDSSGEGPEFVEEEEEVALRSDSEGSDYTPGKKKKKKLGPKKEKKSKSKRKEEEEEEDDDDDSKEPKSSAQLLEDWGMEDIDHVFSEEDYRTLTNYKAFSQFVRPLIAAKNPKIAVSKMMMVLGAKWREFSTNNPFKGSSGASVAAAAAAAVAVVESMVTATEVAPPPPPVEVPIRKAKTKEGKGPNARRKPKGSPRVPDAKKPKPKKVAPLKIKLGGFGSKRKRSSSEDDDLDVESDFDDASINSYSVSDGSTSRSSRSRKKLRTTKKKKKGEEEVTAVDGYETDHQDYCEVCQQGGEIILCDTCPRAYHMVCLDPDMEKAPEGKWSCPHCEKEGIQWEAKEDNSEGEEILEEVGGDLEEEDDHHMEFCRVCKDGGELLCCDTCPSSYHIHCLNPPLPEIPNGEWLCPRCTCPALKGKVQKILIWKWGQPPSPTPVPRPPDADPNTPSPKPLEGRPERQFFVKWQGMSYWHCSWVSELQLELHCQVMFRNYQRKNDMDEPPSGDFGGDEEKSRKRKNKDPKFAEMEERFYRYGIKPEWMMIHRILNHSVDKKGHVHYLIKWRDLPYDQASWESEDVEIQDYDLFKQSYWNHRELMRGEEGRPGKKLKKVKLRKLERPPETPTVDPTVKYERQPEYLDATGGTLHPYQMEGLNWLRFSWAQGTDTILADEMGLGKTVQTAVFLYSLYKEGHSKGPFLVSAPLSTIINWEREFEMWAPDMYVVTYVGDKDSRAIIRENEFSFEDNAIRGGKKASRMKKEASVKFHVLLTSYELITIDMAILGSIDWACLIVDEAHRLKNNQSKFFRVLNGYSLQHKLLLTGTPLQNNLEELFHLLNFLTPERFHNLEGFLEEFADIAKEDQIKKLHDMLGPHMLRRLKADVFKNMPSKTELIVRVELSPMQKKYYKYILTRNFEALNARGGGNQVSLLNVVMDLKKCCNHPYLFPVAAMEAPKMPNGMYDGSALIRASGKLLLLQKMLKNLKEGGHRVLIFSQMTKMLDLLEDFLEHEGYKYERIDGGITGNMRQEAIDRFNAPGAQQFCFLLSTRAGGLGINLATADTVIIYDSDWNPHNDIQAFSRAHRIGQNKKVMIYRFVTRASVEERITQVAKKKMMLTHLVVRPGLGSKTGSMSKQELDDILKFGTEELFKDEATDGGGDNKEGEDSSVIHYDDKAIERLLDRNQDETEDTELQGMNEYLSSFKVAQYVVREEEMGEEEEVEREIIKQEESVDPDYWEKLLRHHYEQQQEDLARNLGKGKRIRKQVNYNDGSQEDRGVCGRPRPPPMGRSTRAVGPAHLPSLPPDWQDDQSDNQSDYSVASEEGDEDFDERSEAPRRPSRKGLRNDKDKPLPPLLARVGGNIEVLGFNARQRKAFLNAIMRYGMPPQDAFTTQWLVRDLRGKSEKEFKAYVSLFMRHLCEPGADGAETFADGVPREGLSRQHVLTRIGVMSLIRKKVQEFEHVNGRWSMPELAEVEENKKMSQPGSPSPKTPTPSTPGDTQPNTPAPVPPAEDGIKIEENSLKEEESIEGEKEVKSTAPETAIECTQAPAPASEDEKVVVEPPEGEEKVEKAEVKERTEEPMETEPKGAADVEKVEEKSAIDLTPIVVEDKEEKKEEEEKKEVMLQNGETPKDLNDEKQKKNIKQRFMFNIADGGFTELHSLWQNEERAATVTKKTYEIWHRRHDYWLLAGIINHGYARWQDIQNDPRYAILNEPFKGEMNRGNFLEIKNKFLARRFKLLEQALVIEEQLRRAAYLNMSEDPSHPSMALNTRFAEVECLAESHQHLSKESMAGNKPANAVLHKVLKQLEELLSDMKADVTRLPATIARIPPVAVRLQMSERNILSRLANRAPEPTPQQVAQQQ

Target ORF information:

RefSeq Version	XM_006718958.1
Organism	Homo sapiens(human)
Definition	Homo sapiens chromodomain helicase DNA binding protein 4 (CHD4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006718958.1

ORF Insert Sequence:

ATGGCGTCGG GCCTGGGCTC CCCGTCCCCC TGCTCGGCGG GCAGTGAGGA GGAGGATATG 
GATGCACTTT TGAACAACAG CCTGCCCCCA CCCCACCCAG AAAATGAAGA GGACCCAGAA 
GAGGATTTGT CAGAAACAGA GACTCCAAAG CTCAAGAAGA AGAAAAAGCC TAAGAAACCT 
CGGGACCCTA AAATCCCTAA GAGCAAGCGC CAAAAAAAGG AGCGTATGCT CTTATGCCGG 
CAGCTGGGGG ACAGCTCTGG GGAGGGGCCA GAGTTTGTGG AGGAGGAGGA AGAGGTGGCT 
CTGCGCTCAG ACAGTGAGGG CAGCGACTAT ACTCCTGGCA AGAAGAAGAA GAAGAAGCTT 
GGACCTAAGA AAGAGAAGAA GAGCAAATCC AAGCGGAAGG AGGAGGAGGA GGAGGAGGAT 
GATGATGATG ATTCAAAGGA GCCTAAATCA TCTGCTCAGC TCCTGGAAGA CTGGGGCATG 
GAAGACATTG ACCACGTGTT CTCAGAGGAG GATTATCGAA CCCTCACCAA CTACAAGGCC 
TTCAGCCAGT TTGTCAGACC CCTCATTGCT GCCAAAAATC CCAAGATTGC TGTCTCCAAG 
ATGATGATGG TTTTGGGTGC AAAATGGCGG GAGTTCAGTA CCAATAACCC CTTCAAAGGC 
AGTTCTGGGG CATCAGTGGC AGCTGCGGCA GCAGCAGCGG TAGCTGTGGT GGAGAGCATG 
GTGACAGCCA CTGAGGTTGC ACCACCACCT CCCCCTGTGG AGGTGCCTAT CCGCAAGGCC 
AAGACCAAGG AGGGCAAAGG TCCCAATGCT CGGAGGAAGC CCAAGGGCAG CCCTCGTGTA 
CCTGATGCCA AGAAGCCTAA ACCCAAGAAA GTAGCTCCCC TGAAAATCAA GCTGGGAGGT 
TTTGGTTCCA AGCGTAAGAG ATCCTCGAGT GAGGATGATG ACTTAGATGT GGAATCTGAC 
TTCGATGATG CCAGTATCAA TAGCTATTCT GTTTCTGATG GTTCCACCAG CCGTAGTAGC 
CGCAGCCGCA AGAAACTCCG AACCACTAAA AAGAAAAAGA AAGGCGAGGA GGAGGTGACT 
GCTGTGGATG GTTATGAGAC AGACCACCAG GACTATTGCG AGGTGTGCCA GCAAGGCGGT 
GAGATCATCC TGTGTGATAC CTGTCCCCGT GCTTACCACA TGGTCTGCCT GGATCCCGAC 
ATGGAGAAGG CTCCCGAGGG CAAGTGGAGC TGCCCACACT GCGAGAAGGA AGGCATCCAG 
TGGGAAGCTA AAGAGGACAA TTCGGAGGGT GAGGAGATCC TGGAAGAGGT TGGGGGAGAC 
CTCGAAGAGG AGGATGACCA CCATATGGAA TTCTGTCGGG TCTGCAAGGA TGGTGGGGAA 
CTGCTCTGCT GTGATACCTG TCCTTCTTCC TACCACATCC ACTGCCTGAA TCCCCCACTT 
CCAGAGATCC CCAACGGTGA ATGGCTCTGT CCCCGTTGTA CGTGTCCAGC TCTGAAGGGC 
AAAGTGCAGA AGATCCTAAT CTGGAAGTGG GGTCAGCCAC CATCTCCCAC ACCAGTGCCT 
CGGCCTCCAG ATGCTGATCC CAACACGCCC TCCCCAAAGC CCTTGGAGGG GCGGCCAGAG 
CGGCAGTTCT TTGTGAAATG GCAAGGCATG TCTTACTGGC ACTGCTCCTG GGTTTCTGAA 
CTGCAGCTGG AGCTGCACTG TCAGGTGATG TTCCGAAACT ATCAGCGGAA GAATGATATG 
GATGAGCCAC CTTCTGGGGA CTTTGGTGGT GATGAAGAGA AAAGCCGAAA GCGAAAGAAC 
AAGGACCCTA AATTTGCAGA GATGGAGGAA CGCTTCTATC GCTATGGGAT AAAACCCGAG 
TGGATGATGA TCCACCGAAT CCTCAACCAC AGTGTGGACA AGAAGGGCCA CGTCCACTAC 
TTGATCAAGT GGCGGGACTT ACCTTACGAT CAGGCTTCTT GGGAGAGTGA GGATGTGGAG 
ATCCAGGATT ACGACCTGTT CAAGCAGAGC TATTGGAATC ACAGGGAGTT AATGAGGGGT 
GAGGAAGGCC GACCAGGCAA GAAGCTCAAG AAGGTGAAGC TTCGGAAGTT GGAGAGGCCT 
CCAGAAACGC CAACAGTTGA TCCAACAGTG AAGTATGAGC GACAGCCAGA GTACCTGGAT 
GCTACAGGTG GAACCCTGCA CCCCTATCAA ATGGAGGGCC TGAATTGGTT GCGCTTCTCC 
TGGGCTCAGG GCACTGACAC CATCTTGGCT GATGAGATGG GCCTTGGGAA AACTGTACAG 
ACAGCAGTCT TCCTGTATTC CCTTTACAAG GAGGGTCATT CCAAAGGCCC CTTCCTAGTG 
AGCGCCCCTC TTTCTACCAT CATCAACTGG GAGCGGGAGT TTGAAATGTG GGCTCCAGAC 
ATGTATGTCG TAACCTATGT GGGTGACAAG GACAGCCGTG CCATCATCCG AGAGAATGAG 
TTCTCCTTTG AAGACAATGC CATTCGTGGT GGCAAGAAGG CCTCCCGCAT GAAGAAAGAG 
GCATCTGTGA AATTCCATGT GCTGCTGACA TCCTATGAAT TGATCACCAT TGACATGGCT 
ATTTTGGGCT CTATTGATTG GGCCTGCCTC ATCGTGGATG AAGCCCATCG GCTGAAGAAC 
AATCAGTCTA AGTTCTTCCG GGTATTGAAT GGTTACTCAC TCCAGCACAA GCTGTTGCTG 
ACTGGGACAC CATTACAAAA CAATCTGGAA GAGTTGTTTC ATCTGCTCAA CTTTCTCACC 
CCCGAGAGGT TCCACAATTT GGAAGGTTTT TTGGAGGAGT TTGCTGACAT TGCCAAGGAG 
GACCAGATAA AAAAACTGCA TGACATGCTG GGGCCGCACA TGTTGCGGCG GCTCAAAGCC 
GATGTGTTCA AGAACATGCC CTCCAAGACA GAACTAATTG TGCGTGTGGA GCTGAGCCCT 
ATGCAGAAGA AATACTACAA GTACATCCTC ACTCGAAATT TTGAAGCACT CAATGCCCGA 
GGTGGTGGCA ACCAGGTGTC TCTGCTGAAT GTGGTGATGG ATCTTAAGAA GTGCTGCAAC 
CATCCATACC TCTTCCCTGT GGCTGCAATG GAAGCTCCTA AGATGCCTAA TGGCATGTAT 
GATGGCAGTG CCCTAATCAG AGCATCTGGG AAATTATTGC TGCTGCAGAA AATGCTCAAG 
AACCTTAAGG AGGGTGGGCA TCGTGTACTC ATCTTTTCCC AGATGACCAA GATGCTAGAC 
CTGCTAGAGG ATTTCTTGGA ACATGAAGGT TATAAATACG AACGCATCGA TGGTGGAATC 
ACTGGGAACA TGCGGCAAGA GGCCATTGAC CGCTTCAATG CACCGGGTGC TCAGCAGTTC 
TGCTTCTTGC TTTCCACTCG AGCTGGGGGC CTTGGAATCA ATCTGGCCAC TGCTGACACA 
GTTATTATCT ATGACTCTGA CTGGAACCCC CATAATGACA TTCAGGCCTT TAGCAGAGCT 
CACCGGATTG GGCAAAATAA AAAGGTAATG ATCTACCGGT TTGTGACCCG TGCGTCAGTG 
GAGGAGCGCA TCACGCAGGT GGCAAAGAAG AAAATGATGC TGACGCATCT AGTGGTGCGG 
CCTGGGCTGG GCTCCAAGAC TGGATCTATG TCCAAACAGG AGCTTGATGA TATCCTCAAA 
TTTGGCACTG AGGAACTATT CAAGGATGAA GCCACTGATG GAGGAGGAGA CAACAAAGAG 
GGAGAAGATA GCAGTGTTAT CCACTACGAT GATAAGGCCA TTGAACGGCT GCTAGACCGT 
AACCAGGATG AGACTGAAGA CACAGAATTG CAGGGCATGA ATGAATATTT GAGCTCATTC 
AAAGTGGCCC AGTATGTGGT ACGGGAAGAA GAAATGGGGG AGGAAGAGGA GGTAGAACGG 
GAAATCATTA AACAGGAAGA AAGTGTGGAT CCTGACTACT GGGAGAAATT GCTGCGGCAC 
CATTATGAGC AGCAGCAAGA AGATCTAGCC CGAAATCTGG GCAAAGGAAA AAGAATCCGT 
AAACAGGTCA ACTACAATGA TGGCTCCCAG GAGGACCGAG GTGTGTGTGG CCGGCCCCGC 
CCCCCACCCA TGGGCCGTTC CACTAGAGCA GTGGGCCCCG CTCATCTGCC CTCTCTCCCT 
CCAGATTGGC AGGACGACCA GTCCGACAAC CAGTCCGATT ACTCAGTGGC TTCAGAGGAA 
GGTGATGAAG ACTTTGATGA ACGTTCAGAA GCTCCCCGTA GGCCCAGTCG TAAGGGCCTG 
CGGAATGATA AAGATAAGCC ATTGCCTCCT CTGTTGGCCC GTGTTGGTGG GAATATTGAA 
GTACTTGGTT TTAATGCTCG TCAGCGAAAA GCCTTTCTTA ATGCAATTAT GCGATATGGT 
ATGCCACCTC AGGATGCTTT TACTACCCAG TGGCTTGTAA GAGACCTGCG AGGCAAATCA 
GAGAAAGAGT TCAAGGCATA TGTCTCTCTT TTCATGCGGC ATTTATGTGA GCCGGGGGCA 
GATGGGGCTG AGACCTTTGC TGATGGTGTC CCCCGAGAAG GCCTGTCTCG CCAGCATGTC 
CTTACTAGAA TTGGTGTTAT GTCTTTGATT CGCAAGAAGG TTCAGGAGTT TGAACATGTT 
AATGGGCGCT GGAGCATGCC TGAACTGGCT GAGGTGGAGG AAAACAAGAA GATGTCCCAG 
CCAGGGTCAC CCTCCCCAAA AACTCCTACA CCCTCCACTC CAGGGGACAC GCAGCCCAAC 
ACTCCTGCAC CTGTCCCACC TGCTGAAGAT GGGATAAAAA TAGAGGAAAA TAGCCTCAAA 
GAAGAAGAGA GCATAGAAGG AGAAAAGGAG GTTAAATCTA CAGCCCCTGA GACTGCCATT 
GAGTGTACAC AGGCCCCTGC CCCTGCCTCA GAGGATGAAA AGGTCGTTGT TGAACCCCCT 
GAGGGAGAGG AGAAAGTGGA AAAGGCAGAG GTGAAGGAGA GAACAGAGGA ACCTATGGAG 
ACAGAGCCCA AAGGTGCTGC TGATGTAGAG AAGGTGGAGG AAAAGTCAGC AATAGATCTG 
ACCCCTATTG TGGTAGAAGA CAAAGAAGAG AAGAAAGAAG AAGAAGAGAA AAAAGAGGTG 
ATGCTTCAGA ATGGAGAGAC CCCCAAGGAC CTGAATGATG AGAAACAGAA GAAAAATATT 
AAACAACGTT TCATGTTTAA CATTGCAGAT GGTGGTTTTA CTGAGTTGCA CTCCCTTTGG 
CAGAATGAAG AGCGGGCAGC CACAGTTACC AAGAAGACTT ATGAGATCTG GCATCGACGG 
CATGACTACT GGCTGCTAGC CGGCATTATA AACCATGGCT ATGCCCGGTG GCAAGACATC 
CAGAATGACC CACGCTATGC CATCCTCAAT GAGCCTTTCA AGGGTGAAAT GAACCGTGGC 
AATTTCTTAG AGATCAAGAA TAAATTTCTA GCTCGAAGGT TTAAGCTCTT AGAACAAGCT 
CTGGTGATTG AGGAACAGCT GCGCCGGGCT GCTTACTTGA ACATGTCAGA AGACCCTTCT 
CACCCTTCCA TGGCCCTCAA CACCCGCTTT GCTGAGGTGG AGTGTTTGGC GGAAAGTCAT 
CAGCACCTGT CCAAGGAGTC AATGGCAGGA AACAAGCCAG CCAATGCAGT CCTGCACAAA 
GTTCTGAAAC AGCTGGAAGA ACTGCTGAGT GACATGAAAG CTGATGTGAC TCGACTCCCA 
GCTACCATTG CCCGAATTCC CCCAGTTGCT GTGAGGTTAC AGATGTCAGA GCGTAACATT 
CTCAGCCGCC TGGCAAACCG GGCACCCGAA CCTACCCCAC AGCAGGTAGC CCAGCAGCAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHD4 ( XM_006718959.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_006719022.1 Homo sapiens chromodomain helicase DNA binding protein 4 (CHD4), transcript variant X6, mRNA.

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CloneID	OHu45951
Clone ID Related Accession (Same CDS sequence)	XM_006718959.1
Accession Version	XM_006718959.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5748bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-25
Organism	Homo sapiens(human)
Product	chromodomain-helicase-DNA-binding protein 4 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCGG GCCTGGGCTC CCCGTCCCCC TGCTCGGCGG GCAGTGAGGA GGAGGATATG 
GATGCACTTT TGAACAACAG CCTGCCCCCA CCCCACCCAG AAAATGAAGA GGACCCAGAA 
GAGGATTTGT CAGAAACAGA GACTCCAAAG CTCAAGAAGA AGAAAAAGCC TAAGAAACCT 
CGGGACCCTA AAATCCCTAA GAGCAAGCGC CAAAAAAAGG AGCGTATGCT CTTATGCCGG 
CAGCTGGGGG ACAGCTCTGG GGAGGGGCCA GAGTTTGTGG AGGAGGAGGA AGAGGTGGCT 
CTGCGCTCAG ACAGTGAGGG CAGCGACTAT ACTCCTGGCA AGAAGAAGAA GAAGAAGCTT 
GGACCTAAGA AAGAGAAGAA GAGCAAATCC AAGCGGAAGG AGGAGGAGGA GGAGGAGGAT 
GATGATGATG ATTCAAAGGA GCCTAAATCA TCTGCTCAGC TCCTGGAAGA CTGGGGCATG 
GAAGACATTG ACCACGTGTT CTCAGAGGAG GATTATCGAA CCCTCACCAA CTACAAGGCC 
TTCAGCCAGT TTGTCAGACC CCTCATTGCT GCCAAAAATC CCAAGATTGC TGTCTCCAAG 
ATGATGATGG TTTTGGGTGC AAAATGGCGG GAGTTCAGTA CCAATAACCC CTTCAAAGGC 
AGTTCTGGGG CATCAGTGGC AGCTGCGGCA GCAGCAGCGG TAGCTGTGGT GGAGAGCATG 
GTGACAGCCA CTGAGGTTGC ACCACCACCT CCCCCTGTGG AGGTGCCTAT CCGCAAGGCC 
AAGACCAAGG AGGGCAAAGG TCCCAATGCT CGGAGGAAGC CCAAGGGCAG CCCTCGTGTA 
CCTGATGCCA AGAAGCCTAA ACCCAAGAAA GTAGCTCCCC TGAAAATCAA GCTGGGAGGT 
TTTGGTTCCA AGCGTAAGAG ATCCTCGAGT GAGGATGATG ACTTAGATGT GGAATCTGAC 
TTCGATGATG CCAGTATCAA TAGCTATTCT GTTTCTGATG GTTCCACCAG CCGTAGTAGC 
CGCAGCCGCA AGAAACTCCG AACCACTAAA AAGAAAAAGA AAGGCGAGGA GGAGGTGACT 
GCTGTGGATG GTTATGAGAC AGACCACCAG GACTATTGCG AGGTGTGCCA GCAAGGCGGT 
GAGATCATCC TGTGTGATAC CTGTCCCCGT GCTTACCACA TGGTCTGCCT GGATCCCGAC 
ATGGAGAAGG CTCCCGAGGG CAAGTGGAGC TGCCCACACT GCGAGAAGGA AGGCATCCAG 
TGGGAAGCTA AAGAGGACAA TTCGGAGGGT GAGGAGATCC TGGAAGAGGT TGGGGGAGAC 
CTCGAAGAGG AGGATGACCA CCATATGGAA TTCTGTCGGG TCTGCAAGGA TGGTGGGGAA 
CTGCTCTGCT GTGATACCTG TCCTTCTTCC TACCACATCC ACTGCCTGAA TCCCCCACTT 
CCAGAGATCC CCAACGGTGA ATGGCTCTGT CCCCGTTGTA CGTGTCCAGC TCTGAAGGGC 
AAAGTGCAGA AGATCCTAAT CTGGAAGTGG GGTCAGCCAC CATCTCCCAC ACCAGTGCCT 
CGGCCTCCAG ATGCTGATCC CAACACGCCC TCCCCAAAGC CCTTGGAGGG GCGGCCAGAG 
CGGCAGTTCT TTGTGAAATG GCAAGGCATG TCTTACTGGC ACTGCTCCTG GGTTTCTGAA 
CTGCAGCTGG AGCTGCACTG TCAGGTGATG TTCCGAAACT ATCAGCGGAA GAATGATATG 
GATGAGCCAC CTTCTGGGGA CTTTGGTGGT GATGAAGAGA AAAGCCGAAA GCGAAAGAAC 
AAGGACCCTA AATTTGCAGA GATGGAGGAA CGCTTCTATC GCTATGGGAT AAAACCCGAG 
TGGATGATGA TCCACCGAAT CCTCAACCAC AGTGTGGACA AGAAGGGCCA CGTCCACTAC 
TTGATCAAGT GGCGGGACTT ACCTTACGAT CAGGCTTCTT GGGAGAGTGA GGATGTGGAG 
ATCCAGGATT ACGACCTGTT CAAGCAGAGC TATTGGAATC ACAGGGAGTT AATGAGGGGT 
GAGGAAGGCC GACCAGGCAA GAAGCTCAAG AAGGTGAAGC TTCGGAAGTT GGAGAGGCCT 
CCAGAAACGC CAACAGTTGA TCCAACAGTG AAGTATGAGC GACAGCCAGA GTACCTGGAT 
GCTACAGGTG GAACCCTGCA CCCCTATCAA ATGGAGGGCC TGAATTGGTT GCGCTTCTCC 
TGGGCTCAGG GCACTGACAC CATCTTGGCT GATGAGATGG GCCTTGGGAA AACTGTACAG 
ACAGCAGTCT TCCTGTATTC CCTTTACAAG GAGGGTCATT CCAAAGGCCC CTTCCTAGTG 
AGCGCCCCTC TTTCTACCAT CATCAACTGG GAGCGGGAGT TTGAAATGTG GGCTCCAGAC 
ATGTATGTCG TAACCTATGT GGGTGACAAG GACAGCCGTG CCATCATCCG AGAGAATGAG 
TTCTCCTTTG AAGACAATGC CATTCGTGGT GGCAAGAAGG CCTCCCGCAT GAAGAAAGAG 
GCATCTGTGA AATTCCATGT GCTGCTGACA TCCTATGAAT TGATCACCAT TGACATGGCT 
ATTTTGGGCT CTATTGATTG GGCCTGCCTC ATCGTGGATG AAGCCCATCG GCTGAAGAAC 
AATCAGTCTA AGTTCTTCCG GGTATTGAAT GGTTACTCAC TCCAGCACAA GCTGTTGCTG 
ACTGGGACAC CATTACAAAA CAATCTGGAA GAGTTGTTTC ATCTGCTCAA CTTTCTCACC 
CCCGAGAGGT TCCACAATTT GGAAGGTTTT TTGGAGGAGT TTGCTGACAT TGCCAAGGAG 
GACCAGATAA AAAAACTGCA TGACATGCTG GGGCCGCACA TGTTGCGGCG GCTCAAAGCC 
GATGTGTTCA AGAACATGCC CTCCAAGACA GAACTAATTG TGCGTGTGGA GCTGAGCCCT 
ATGCAGAAGA AATACTACAA GTACATCCTC ACTCGAAATT TTGAAGCACT CAATGCCCGA 
GGTGGTGGCA ACCAGGTGTC TCTGCTGAAT GTGGTGATGG ATCTTAAGAA GTGCTGCAAC 
CATCCATACC TCTTCCCTGT GGCTGCAATG GAAGCTCCTA AGATGCCTAA TGGCATGTAT 
GATGGCAGTG CCCTAATCAG AGCATCTGGG AAATTATTGC TGCTGCAGAA AATGCTCAAG 
AACCTTAAGG AGGGTGGGCA TCGTGTACTC ATCTTTTCCC AGATGACCAA GATGCTAGAC 
CTGCTAGAGG ATTTCTTGGA ACATGAAGGT TATAAATACG AACGCATCGA TGGTGGAATC 
ACTGGGAACA TGCGGCAAGA GGCCATTGAC CGCTTCAATG CACCGGGTGC TCAGCAGTTC 
TGCTTCTTGC TTTCCACTCG AGCTGGGGGC CTTGGAATCA ATCTGGCCAC TGCTGACACA 
GTTATTATCT ATGACTCTGA CTGGAACCCC CATAATGACA TTCAGGCCTT TAGCAGAGCT 
CACCGGATTG GGCAAAATAA AAAGGTAATG ATCTACCGGT TTGTGACCCG TGCGTCAGTG 
GAGGAGCGCA TCACGCAGGT GGCAAAGAAG AAAATGATGC TGACGCATCT AGTGGTGCGG 
CCTGGGCTGG GCTCCAAGAC TGGATCTATG TCCAAACAGG AGCTTGATGA TATCCTCAAA 
TTTGGCACTG AGGAACTATT CAAGGATGAA GCCACTGATG GAGGAGGAGA CAACAAAGAG 
GGAGAAGATA GCAGTGTTAT CCACTACGAT GATAAGGCCA TTGAACGGCT GCTAGACCGT 
AACCAGGATG AGACTGAAGA CACAGAATTG CAGGGCATGA ATGAATATTT GAGCTCATTC 
AAAGTGGCCC AGTATGTGGT ACGGGAAGAA GAAATGGGGG AGGAAGAGGA GGTAGAACGG 
GAAATCATTA AACAGGAAGA AAGTGTGGAT CCTGACTACT GGGAGAAATT GCTGCGGCAC 
CATTATGAGC AGCAGCAAGA AGATCTAGCC CGAAATCTGG GCAAAGGAAA AAGAATCCGT 
AAACAGGTCA ACTACAATGA TGGCTCCCAG GAGGACCGAG ATTGGCAGGA CGACCAGTCC 
GACAACCAGT CCGATTACTC AGTGGCTTCA GAGGAAGGTG ATGAAGACTT TGATGAACGT 
TCAGAAGCTC CCCGTAGGCC CAGTCGTAAG GGCCTGCGGA ATGATAAAGA TAAGCCATTG 
CCTCCTCTGT TGGCCCGTGT TGGTGGGAAT ATTGAAGTAC TTGGTTTTAA TGCTCGTCAG 
CGAAAAGCCT TTCTTAATGC AATTATGCGA TATGGTATGC CACCTCAGGA TGCTTTTACT 
ACCCAGTGGC TTGTAAGAGA CCTGCGAGGC AAATCAGAGA AAGAGTTCAA GGCATATGTC 
TCTCTTTTCA TGCGGCATTT ATGTGAGCCG GGGGCAGATG GGGCTGAGAC CTTTGCTGAT 
GGTGTCCCCC GAGAAGGCCT GTCTCGCCAG CATGTCCTTA CTAGAATTGG TGTTATGTCT 
TTGATTCGCA AGAAGGTTCA GGAGTTTGAA CATGTTAATG GGCGCTGGAG CATGCCTGAA 
CTGGCTGAGG TGGAGGAAAA CAAGAAGATG TCCCAGCCAG GGTCACCCTC CCCAAAAACT 
CCTACACCCT CCACTCCAGG GGACACGCAG CCCAACACTC CTGCACCTGT CCCACCTGCT 
GAAGATGGGA TAAAAATAGA GGAAAATAGC CTCAAAGAAG AAGAGAGCAT AGAAGGAGAA 
AAGGAGGTTA AATCTACAGC CCCTGAGACT GCCATTGAGT GTACACAGGC CCCTGCCCCT 
GCCTCAGAGG ATGAAAAGGT CGTTGTTGAA CCCCCTGAGG GAGAGGAGAA AGTGGAAAAG 
GCAGAGGTGA AGGAGAGAAC AGAGGAACCT ATGGAGACAG AGCCCAAAGG TAAAGGTGCT 
GCTGATGTAG AGAAGGTGGA GGAAAAGTCA GCAATAGATC TGACCCCTAT TGTGGTAGAA 
GACAAAGAAG AGAAGAAAGA AGAAGAAGAG AAAAAAGAGG TGATGCTTCA GAATGGAGAG 
ACCCCCAAGG ACCTGAATGA TGAGAAACAG AAGAAAAATA TTAAACAACG TTTCATGTTT 
AACATTGCAG ATGGTGGTTT TACTGAGTTG CACTCCCTTT GGCAGAATGA AGAGCGGGCA 
GCCACAGTTA CCAAGAAGAC TTATGAGATC TGGCATCGAC GGCATGACTA CTGGCTGCTA 
GCCGGCATTA TAAACCATGG CTATGCCCGG TGGCAAGACA TCCAGAATGA CCCACGCTAT 
GCCATCCTCA ATGAGCCTTT CAAGGGTGAA ATGAACCGTG GCAATTTCTT AGAGATCAAG 
AATAAATTTC TAGCTCGAAG GTTTAAGCTC TTAGAACAAG CTCTGGTGAT TGAGGAACAG 
CTGCGCCGGG CTGCTTACTT GAACATGTCA GAAGACCCTT CTCACCCTTC CATGGCCCTC 
AACACCCGCT TTGCTGAGGT GGAGTGTTTG GCGGAAAGTC ATCAGCACCT GTCCAAGGAG 
TCAATGGCAG GAAACAAGCC AGCCAATGCA GTCCTGCACA AAGGTATTCT GAAACAGCTG 
GAAGAACTGC TGAGTGACAT GAAAGCTGAT GTGACTCGAC TCCCAGCTAC CATTGCCCGA 
ATTCCCCCAG TTGCTGTGAG GTTACAGATG TCAGAGCGTA ACATTCTCAG CCGCCTGGCA 
AACCGGGCAC CCGAACCTAC CCCACAGCAG GTAGCCCAGC AGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006719022.1
CDS	219..5966
Translation	MASGLGSPSPCSAGSEEEDMDALLNNSLPPPHPENEEDPEEDLSETETPKLKKKKKPKKPRDPKIPKSKRQKKERMLLCRQLGDSSGEGPEFVEEEEEVALRSDSEGSDYTPGKKKKKKLGPKKEKKSKSKRKEEEEEEDDDDDSKEPKSSAQLLEDWGMEDIDHVFSEEDYRTLTNYKAFSQFVRPLIAAKNPKIAVSKMMMVLGAKWREFSTNNPFKGSSGASVAAAAAAAVAVVESMVTATEVAPPPPPVEVPIRKAKTKEGKGPNARRKPKGSPRVPDAKKPKPKKVAPLKIKLGGFGSKRKRSSSEDDDLDVESDFDDASINSYSVSDGSTSRSSRSRKKLRTTKKKKKGEEEVTAVDGYETDHQDYCEVCQQGGEIILCDTCPRAYHMVCLDPDMEKAPEGKWSCPHCEKEGIQWEAKEDNSEGEEILEEVGGDLEEEDDHHMEFCRVCKDGGELLCCDTCPSSYHIHCLNPPLPEIPNGEWLCPRCTCPALKGKVQKILIWKWGQPPSPTPVPRPPDADPNTPSPKPLEGRPERQFFVKWQGMSYWHCSWVSELQLELHCQVMFRNYQRKNDMDEPPSGDFGGDEEKSRKRKNKDPKFAEMEERFYRYGIKPEWMMIHRILNHSVDKKGHVHYLIKWRDLPYDQASWESEDVEIQDYDLFKQSYWNHRELMRGEEGRPGKKLKKVKLRKLERPPETPTVDPTVKYERQPEYLDATGGTLHPYQMEGLNWLRFSWAQGTDTILADEMGLGKTVQTAVFLYSLYKEGHSKGPFLVSAPLSTIINWEREFEMWAPDMYVVTYVGDKDSRAIIRENEFSFEDNAIRGGKKASRMKKEASVKFHVLLTSYELITIDMAILGSIDWACLIVDEAHRLKNNQSKFFRVLNGYSLQHKLLLTGTPLQNNLEELFHLLNFLTPERFHNLEGFLEEFADIAKEDQIKKLHDMLGPHMLRRLKADVFKNMPSKTELIVRVELSPMQKKYYKYILTRNFEALNARGGGNQVSLLNVVMDLKKCCNHPYLFPVAAMEAPKMPNGMYDGSALIRASGKLLLLQKMLKNLKEGGHRVLIFSQMTKMLDLLEDFLEHEGYKYERIDGGITGNMRQEAIDRFNAPGAQQFCFLLSTRAGGLGINLATADTVIIYDSDWNPHNDIQAFSRAHRIGQNKKVMIYRFVTRASVEERITQVAKKKMMLTHLVVRPGLGSKTGSMSKQELDDILKFGTEELFKDEATDGGGDNKEGEDSSVIHYDDKAIERLLDRNQDETEDTELQGMNEYLSSFKVAQYVVREEEMGEEEEVEREIIKQEESVDPDYWEKLLRHHYEQQQEDLARNLGKGKRIRKQVNYNDGSQEDRDWQDDQSDNQSDYSVASEEGDEDFDERSEAPRRPSRKGLRNDKDKPLPPLLARVGGNIEVLGFNARQRKAFLNAIMRYGMPPQDAFTTQWLVRDLRGKSEKEFKAYVSLFMRHLCEPGADGAETFADGVPREGLSRQHVLTRIGVMSLIRKKVQEFEHVNGRWSMPELAEVEENKKMSQPGSPSPKTPTPSTPGDTQPNTPAPVPPAEDGIKIEENSLKEEESIEGEKEVKSTAPETAIECTQAPAPASEDEKVVVEPPEGEEKVEKAEVKERTEEPMETEPKGKGAADVEKVEEKSAIDLTPIVVEDKEEKKEEEEKKEVMLQNGETPKDLNDEKQKKNIKQRFMFNIADGGFTELHSLWQNEERAATVTKKTYEIWHRRHDYWLLAGIINHGYARWQDIQNDPRYAILNEPFKGEMNRGNFLEIKNKFLARRFKLLEQALVIEEQLRRAAYLNMSEDPSHPSMALNTRFAEVECLAESHQHLSKESMAGNKPANAVLHKGILKQLEELLSDMKADVTRLPATIARIPPVAVRLQMSERNILSRLANRAPEPTPQQVAQQQ

Target ORF information:

RefSeq Version	XM_006718959.1
Organism	Homo sapiens(human)
Definition	Homo sapiens chromodomain helicase DNA binding protein 4 (CHD4), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006718959.1

ORF Insert Sequence:

ATGGCGTCGG GCCTGGGCTC CCCGTCCCCC TGCTCGGCGG GCAGTGAGGA GGAGGATATG 
GATGCACTTT TGAACAACAG CCTGCCCCCA CCCCACCCAG AAAATGAAGA GGACCCAGAA 
GAGGATTTGT CAGAAACAGA GACTCCAAAG CTCAAGAAGA AGAAAAAGCC TAAGAAACCT 
CGGGACCCTA AAATCCCTAA GAGCAAGCGC CAAAAAAAGG AGCGTATGCT CTTATGCCGG 
CAGCTGGGGG ACAGCTCTGG GGAGGGGCCA GAGTTTGTGG AGGAGGAGGA AGAGGTGGCT 
CTGCGCTCAG ACAGTGAGGG CAGCGACTAT ACTCCTGGCA AGAAGAAGAA GAAGAAGCTT 
GGACCTAAGA AAGAGAAGAA GAGCAAATCC AAGCGGAAGG AGGAGGAGGA GGAGGAGGAT 
GATGATGATG ATTCAAAGGA GCCTAAATCA TCTGCTCAGC TCCTGGAAGA CTGGGGCATG 
GAAGACATTG ACCACGTGTT CTCAGAGGAG GATTATCGAA CCCTCACCAA CTACAAGGCC 
TTCAGCCAGT TTGTCAGACC CCTCATTGCT GCCAAAAATC CCAAGATTGC TGTCTCCAAG 
ATGATGATGG TTTTGGGTGC AAAATGGCGG GAGTTCAGTA CCAATAACCC CTTCAAAGGC 
AGTTCTGGGG CATCAGTGGC AGCTGCGGCA GCAGCAGCGG TAGCTGTGGT GGAGAGCATG 
GTGACAGCCA CTGAGGTTGC ACCACCACCT CCCCCTGTGG AGGTGCCTAT CCGCAAGGCC 
AAGACCAAGG AGGGCAAAGG TCCCAATGCT CGGAGGAAGC CCAAGGGCAG CCCTCGTGTA 
CCTGATGCCA AGAAGCCTAA ACCCAAGAAA GTAGCTCCCC TGAAAATCAA GCTGGGAGGT 
TTTGGTTCCA AGCGTAAGAG ATCCTCGAGT GAGGATGATG ACTTAGATGT GGAATCTGAC 
TTCGATGATG CCAGTATCAA TAGCTATTCT GTTTCTGATG GTTCCACCAG CCGTAGTAGC 
CGCAGCCGCA AGAAACTCCG AACCACTAAA AAGAAAAAGA AAGGCGAGGA GGAGGTGACT 
GCTGTGGATG GTTATGAGAC AGACCACCAG GACTATTGCG AGGTGTGCCA GCAAGGCGGT 
GAGATCATCC TGTGTGATAC CTGTCCCCGT GCTTACCACA TGGTCTGCCT GGATCCCGAC 
ATGGAGAAGG CTCCCGAGGG CAAGTGGAGC TGCCCACACT GCGAGAAGGA AGGCATCCAG 
TGGGAAGCTA AAGAGGACAA TTCGGAGGGT GAGGAGATCC TGGAAGAGGT TGGGGGAGAC 
CTCGAAGAGG AGGATGACCA CCATATGGAA TTCTGTCGGG TCTGCAAGGA TGGTGGGGAA 
CTGCTCTGCT GTGATACCTG TCCTTCTTCC TACCACATCC ACTGCCTGAA TCCCCCACTT 
CCAGAGATCC CCAACGGTGA ATGGCTCTGT CCCCGTTGTA CGTGTCCAGC TCTGAAGGGC 
AAAGTGCAGA AGATCCTAAT CTGGAAGTGG GGTCAGCCAC CATCTCCCAC ACCAGTGCCT 
CGGCCTCCAG ATGCTGATCC CAACACGCCC TCCCCAAAGC CCTTGGAGGG GCGGCCAGAG 
CGGCAGTTCT TTGTGAAATG GCAAGGCATG TCTTACTGGC ACTGCTCCTG GGTTTCTGAA 
CTGCAGCTGG AGCTGCACTG TCAGGTGATG TTCCGAAACT ATCAGCGGAA GAATGATATG 
GATGAGCCAC CTTCTGGGGA CTTTGGTGGT GATGAAGAGA AAAGCCGAAA GCGAAAGAAC 
AAGGACCCTA AATTTGCAGA GATGGAGGAA CGCTTCTATC GCTATGGGAT AAAACCCGAG 
TGGATGATGA TCCACCGAAT CCTCAACCAC AGTGTGGACA AGAAGGGCCA CGTCCACTAC 
TTGATCAAGT GGCGGGACTT ACCTTACGAT CAGGCTTCTT GGGAGAGTGA GGATGTGGAG 
ATCCAGGATT ACGACCTGTT CAAGCAGAGC TATTGGAATC ACAGGGAGTT AATGAGGGGT 
GAGGAAGGCC GACCAGGCAA GAAGCTCAAG AAGGTGAAGC TTCGGAAGTT GGAGAGGCCT 
CCAGAAACGC CAACAGTTGA TCCAACAGTG AAGTATGAGC GACAGCCAGA GTACCTGGAT 
GCTACAGGTG GAACCCTGCA CCCCTATCAA ATGGAGGGCC TGAATTGGTT GCGCTTCTCC 
TGGGCTCAGG GCACTGACAC CATCTTGGCT GATGAGATGG GCCTTGGGAA AACTGTACAG 
ACAGCAGTCT TCCTGTATTC CCTTTACAAG GAGGGTCATT CCAAAGGCCC CTTCCTAGTG 
AGCGCCCCTC TTTCTACCAT CATCAACTGG GAGCGGGAGT TTGAAATGTG GGCTCCAGAC 
ATGTATGTCG TAACCTATGT GGGTGACAAG GACAGCCGTG CCATCATCCG AGAGAATGAG 
TTCTCCTTTG AAGACAATGC CATTCGTGGT GGCAAGAAGG CCTCCCGCAT GAAGAAAGAG 
GCATCTGTGA AATTCCATGT GCTGCTGACA TCCTATGAAT TGATCACCAT TGACATGGCT 
ATTTTGGGCT CTATTGATTG GGCCTGCCTC ATCGTGGATG AAGCCCATCG GCTGAAGAAC 
AATCAGTCTA AGTTCTTCCG GGTATTGAAT GGTTACTCAC TCCAGCACAA GCTGTTGCTG 
ACTGGGACAC CATTACAAAA CAATCTGGAA GAGTTGTTTC ATCTGCTCAA CTTTCTCACC 
CCCGAGAGGT TCCACAATTT GGAAGGTTTT TTGGAGGAGT TTGCTGACAT TGCCAAGGAG 
GACCAGATAA AAAAACTGCA TGACATGCTG GGGCCGCACA TGTTGCGGCG GCTCAAAGCC 
GATGTGTTCA AGAACATGCC CTCCAAGACA GAACTAATTG TGCGTGTGGA GCTGAGCCCT 
ATGCAGAAGA AATACTACAA GTACATCCTC ACTCGAAATT TTGAAGCACT CAATGCCCGA 
GGTGGTGGCA ACCAGGTGTC TCTGCTGAAT GTGGTGATGG ATCTTAAGAA GTGCTGCAAC 
CATCCATACC TCTTCCCTGT GGCTGCAATG GAAGCTCCTA AGATGCCTAA TGGCATGTAT 
GATGGCAGTG CCCTAATCAG AGCATCTGGG AAATTATTGC TGCTGCAGAA AATGCTCAAG 
AACCTTAAGG AGGGTGGGCA TCGTGTACTC ATCTTTTCCC AGATGACCAA GATGCTAGAC 
CTGCTAGAGG ATTTCTTGGA ACATGAAGGT TATAAATACG AACGCATCGA TGGTGGAATC 
ACTGGGAACA TGCGGCAAGA GGCCATTGAC CGCTTCAATG CACCGGGTGC TCAGCAGTTC 
TGCTTCTTGC TTTCCACTCG AGCTGGGGGC CTTGGAATCA ATCTGGCCAC TGCTGACACA 
GTTATTATCT ATGACTCTGA CTGGAACCCC CATAATGACA TTCAGGCCTT TAGCAGAGCT 
CACCGGATTG GGCAAAATAA AAAGGTAATG ATCTACCGGT TTGTGACCCG TGCGTCAGTG 
GAGGAGCGCA TCACGCAGGT GGCAAAGAAG AAAATGATGC TGACGCATCT AGTGGTGCGG 
CCTGGGCTGG GCTCCAAGAC TGGATCTATG TCCAAACAGG AGCTTGATGA TATCCTCAAA 
TTTGGCACTG AGGAACTATT CAAGGATGAA GCCACTGATG GAGGAGGAGA CAACAAAGAG 
GGAGAAGATA GCAGTGTTAT CCACTACGAT GATAAGGCCA TTGAACGGCT GCTAGACCGT 
AACCAGGATG AGACTGAAGA CACAGAATTG CAGGGCATGA ATGAATATTT GAGCTCATTC 
AAAGTGGCCC AGTATGTGGT ACGGGAAGAA GAAATGGGGG AGGAAGAGGA GGTAGAACGG 
GAAATCATTA AACAGGAAGA AAGTGTGGAT CCTGACTACT GGGAGAAATT GCTGCGGCAC 
CATTATGAGC AGCAGCAAGA AGATCTAGCC CGAAATCTGG GCAAAGGAAA AAGAATCCGT 
AAACAGGTCA ACTACAATGA TGGCTCCCAG GAGGACCGAG ATTGGCAGGA CGACCAGTCC 
GACAACCAGT CCGATTACTC AGTGGCTTCA GAGGAAGGTG ATGAAGACTT TGATGAACGT 
TCAGAAGCTC CCCGTAGGCC CAGTCGTAAG GGCCTGCGGA ATGATAAAGA TAAGCCATTG 
CCTCCTCTGT TGGCCCGTGT TGGTGGGAAT ATTGAAGTAC TTGGTTTTAA TGCTCGTCAG 
CGAAAAGCCT TTCTTAATGC AATTATGCGA TATGGTATGC CACCTCAGGA TGCTTTTACT 
ACCCAGTGGC TTGTAAGAGA CCTGCGAGGC AAATCAGAGA AAGAGTTCAA GGCATATGTC 
TCTCTTTTCA TGCGGCATTT ATGTGAGCCG GGGGCAGATG GGGCTGAGAC CTTTGCTGAT 
GGTGTCCCCC GAGAAGGCCT GTCTCGCCAG CATGTCCTTA CTAGAATTGG TGTTATGTCT 
TTGATTCGCA AGAAGGTTCA GGAGTTTGAA CATGTTAATG GGCGCTGGAG CATGCCTGAA 
CTGGCTGAGG TGGAGGAAAA CAAGAAGATG TCCCAGCCAG GGTCACCCTC CCCAAAAACT 
CCTACACCCT CCACTCCAGG GGACACGCAG CCCAACACTC CTGCACCTGT CCCACCTGCT 
GAAGATGGGA TAAAAATAGA GGAAAATAGC CTCAAAGAAG AAGAGAGCAT AGAAGGAGAA 
AAGGAGGTTA AATCTACAGC CCCTGAGACT GCCATTGAGT GTACACAGGC CCCTGCCCCT 
GCCTCAGAGG ATGAAAAGGT CGTTGTTGAA CCCCCTGAGG GAGAGGAGAA AGTGGAAAAG 
GCAGAGGTGA AGGAGAGAAC AGAGGAACCT ATGGAGACAG AGCCCAAAGG TAAAGGTGCT 
GCTGATGTAG AGAAGGTGGA GGAAAAGTCA GCAATAGATC TGACCCCTAT TGTGGTAGAA 
GACAAAGAAG AGAAGAAAGA AGAAGAAGAG AAAAAAGAGG TGATGCTTCA GAATGGAGAG 
ACCCCCAAGG ACCTGAATGA TGAGAAACAG AAGAAAAATA TTAAACAACG TTTCATGTTT 
AACATTGCAG ATGGTGGTTT TACTGAGTTG CACTCCCTTT GGCAGAATGA AGAGCGGGCA 
GCCACAGTTA CCAAGAAGAC TTATGAGATC TGGCATCGAC GGCATGACTA CTGGCTGCTA 
GCCGGCATTA TAAACCATGG CTATGCCCGG TGGCAAGACA TCCAGAATGA CCCACGCTAT 
GCCATCCTCA ATGAGCCTTT CAAGGGTGAA ATGAACCGTG GCAATTTCTT AGAGATCAAG 
AATAAATTTC TAGCTCGAAG GTTTAAGCTC TTAGAACAAG CTCTGGTGAT TGAGGAACAG 
CTGCGCCGGG CTGCTTACTT GAACATGTCA GAAGACCCTT CTCACCCTTC CATGGCCCTC 
AACACCCGCT TTGCTGAGGT GGAGTGTTTG GCGGAAAGTC ATCAGCACCT GTCCAAGGAG 
TCAATGGCAG GAAACAAGCC AGCCAATGCA GTCCTGCACA AAGGTATTCT GAAACAGCTG 
GAAGAACTGC TGAGTGACAT GAAAGCTGAT GTGACTCGAC TCCCAGCTAC CATTGCCCGA 
ATTCCCCCAG TTGCTGTGAG GTTACAGATG TCAGAGCGTA ACATTCTCAG CCGCCTGGCA 
AACCGGGCAC CCGAACCTAC CCCACAGCAG GTAGCCCAGC AGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHD4 ( XM_006718960.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_006719023.1 Homo sapiens chromodomain helicase DNA binding protein 4 (CHD4), transcript variant X8, mRNA.

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CloneID	OHu45952
Clone ID Related Accession (Same CDS sequence)	XM_006718960.1
Accession Version	XM_006718960.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5745bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-25
Organism	Homo sapiens(human)
Product	chromodomain-helicase-DNA-binding protein 4 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCGG GCCTGGGCTC CCCGTCCCCC TGCTCGGCGG GCAGTGAGGA GGAGGATATG 
GATGCACTTT TGAACAACAG CCTGCCCCCA CCCCACCCAG AAAATGAAGA GGACCCAGAA 
GAGGATTTGT CAGAAACAGA GACTCCAAAG CTCAAGAAGA AGAAAAAGCC TAAGAAACCT 
CGGGACCCTA AAATCCCTAA GAGCAAGCGC CAAAAAAAGG AGCGTATGCT CTTATGCCGG 
CAGCTGGGGG ACAGCTCTGG GGAGGGGCCA GAGTTTGTGG AGGAGGAGGA AGAGGTGGCT 
CTGCGCTCAG ACAGTGAGGG CAGCGACTAT ACTCCTGGCA AGAAGAAGAA GAAGAAGCTT 
GGACCTAAGA AAGAGAAGAA GAGCAAATCC AAGCGGAAGG AGGAGGAGGA GGAGGAGGAT 
GATGATGATG ATTCAAAGGA GCCTAAATCA TCTGCTCAGC TCCTGGAAGA CTGGGGCATG 
GAAGACATTG ACCACGTGTT CTCAGAGGAG GATTATCGAA CCCTCACCAA CTACAAGGCC 
TTCAGCCAGT TTGTCAGACC CCTCATTGCT GCCAAAAATC CCAAGATTGC TGTCTCCAAG 
ATGATGATGG TTTTGGGTGC AAAATGGCGG GAGTTCAGTA CCAATAACCC CTTCAAAGGC 
AGTTCTGGGG CATCAGTGGC AGCTGCGGCA GCAGCAGCGG TAGCTGTGGT GGAGAGCATG 
GTGACAGCCA CTGAGGTTGC ACCACCACCT CCCCCTGTGG AGGTGCCTAT CCGCAAGGCC 
AAGACCAAGG AGGGCAAAGG TCCCAATGCT CGGAGGAAGC CCAAGGGCAG CCCTCGTGTA 
CCTGATGCCA AGAAGCCTAA ACCCAAGAAA GTAGCTCCCC TGAAAATCAA GCTGGGAGGT 
TTTGGTTCCA AGCGTAAGAG ATCCTCGAGT GAGGATGATG ACTTAGATGT GGAATCTGAC 
TTCGATGATG CCAGTATCAA TAGCTATTCT GTTTCTGATG GTTCCACCAG CCGTAGTAGC 
CGCAGCCGCA AGAAACTCCG AACCACTAAA AAGAAAAAGA AAGGCGAGGA GGAGGTGACT 
GCTGTGGATG GTTATGAGAC AGACCACCAG GACTATTGCG AGGTGTGCCA GCAAGGCGGT 
GAGATCATCC TGTGTGATAC CTGTCCCCGT GCTTACCACA TGGTCTGCCT GGATCCCGAC 
ATGGAGAAGG CTCCCGAGGG CAAGTGGAGC TGCCCACACT GCGAGAAGGA AGGCATCCAG 
TGGGAAGCTA AAGAGGACAA TTCGGAGGGT GAGGAGATCC TGGAAGAGGT TGGGGGAGAC 
CTCGAAGAGG AGGATGACCA CCATATGGAA TTCTGTCGGG TCTGCAAGGA TGGTGGGGAA 
CTGCTCTGCT GTGATACCTG TCCTTCTTCC TACCACATCC ACTGCCTGAA TCCCCCACTT 
CCAGAGATCC CCAACGGTGA ATGGCTCTGT CCCCGTTGTA CGTGTCCAGC TCTGAAGGGC 
AAAGTGCAGA AGATCCTAAT CTGGAAGTGG GGTCAGCCAC CATCTCCCAC ACCAGTGCCT 
CGGCCTCCAG ATGCTGATCC CAACACGCCC TCCCCAAAGC CCTTGGAGGG GCGGCCAGAG 
CGGCAGTTCT TTGTGAAATG GCAAGGCATG TCTTACTGGC ACTGCTCCTG GGTTTCTGAA 
CTGCAGCTGG AGCTGCACTG TCAGGTGATG TTCCGAAACT ATCAGCGGAA GAATGATATG 
GATGAGCCAC CTTCTGGGGA CTTTGGTGGT GATGAAGAGA AAAGCCGAAA GCGAAAGAAC 
AAGGACCCTA AATTTGCAGA GATGGAGGAA CGCTTCTATC GCTATGGGAT AAAACCCGAG 
TGGATGATGA TCCACCGAAT CCTCAACCAC AGTGTGGACA AGAAGGGCCA CGTCCACTAC 
TTGATCAAGT GGCGGGACTT ACCTTACGAT CAGGCTTCTT GGGAGAGTGA GGATGTGGAG 
ATCCAGGATT ACGACCTGTT CAAGCAGAGC TATTGGAATC ACAGGGAGTT AATGAGGGGT 
GAGGAAGGCC GACCAGGCAA GAAGCTCAAG AAGGTGAAGC TTCGGAAGTT GGAGAGGCCT 
CCAGAAACGC CAACAGTTGA TCCAACAGTG AAGTATGAGC GACAGCCAGA GTACCTGGAT 
GCTACAGGTG GAACCCTGCA CCCCTATCAA ATGGAGGGCC TGAATTGGTT GCGCTTCTCC 
TGGGCTCAGG GCACTGACAC CATCTTGGCT GATGAGATGG GCCTTGGGAA AACTGTACAG 
ACAGCAGTCT TCCTGTATTC CCTTTACAAG GAGGGTCATT CCAAAGGCCC CTTCCTAGTG 
AGCGCCCCTC TTTCTACCAT CATCAACTGG GAGCGGGAGT TTGAAATGTG GGCTCCAGAC 
ATGTATGTCG TAACCTATGT GGGTGACAAG GACAGCCGTG CCATCATCCG AGAGAATGAG 
TTCTCCTTTG AAGACAATGC CATTCGTGGT GGCAAGAAGG CCTCCCGCAT GAAGAAAGAG 
GCATCTGTGA AATTCCATGT GCTGCTGACA TCCTATGAAT TGATCACCAT TGACATGGCT 
ATTTTGGGCT CTATTGATTG GGCCTGCCTC ATCGTGGATG AAGCCCATCG GCTGAAGAAC 
AATCAGTCTA AGTTCTTCCG GGTATTGAAT GGTTACTCAC TCCAGCACAA GCTGTTGCTG 
ACTGGGACAC CATTACAAAA CAATCTGGAA GAGTTGTTTC ATCTGCTCAA CTTTCTCACC 
CCCGAGAGGT TCCACAATTT GGAAGGTTTT TTGGAGGAGT TTGCTGACAT TGCCAAGGAG 
GACCAGATAA AAAAACTGCA TGACATGCTG GGGCCGCACA TGTTGCGGCG GCTCAAAGCC 
GATGTGTTCA AGAACATGCC CTCCAAGACA GAACTAATTG TGCGTGTGGA GCTGAGCCCT 
ATGCAGAAGA AATACTACAA GTACATCCTC ACTCGAAATT TTGAAGCACT CAATGCCCGA 
GGTGGTGGCA ACCAGGTGTC TCTGCTGAAT GTGGTGATGG ATCTTAAGAA GTGCTGCAAC 
CATCCATACC TCTTCCCTGT GGCTGCAATG GAAGCTCCTA AGATGCCTAA TGGCATGTAT 
GATGGCAGTG CCCTAATCAG AGCATCTGGG AAATTATTGC TGCTGCAGAA AATGCTCAAG 
AACCTTAAGG AGGGTGGGCA TCGTGTACTC ATCTTTTCCC AGATGACCAA GATGCTAGAC 
CTGCTAGAGG ATTTCTTGGA ACATGAAGGT TATAAATACG AACGCATCGA TGGTGGAATC 
ACTGGGAACA TGCGGCAAGA GGCCATTGAC CGCTTCAATG CACCGGGTGC TCAGCAGTTC 
TGCTTCTTGC TTTCCACTCG AGCTGGGGGC CTTGGAATCA ATCTGGCCAC TGCTGACACA 
GTTATTATCT ATGACTCTGA CTGGAACCCC CATAATGACA TTCAGGCCTT TAGCAGAGCT 
CACCGGATTG GGCAAAATAA AAAGGTAATG ATCTACCGGT TTGTGACCCG TGCGTCAGTG 
GAGGAGCGCA TCACGCAGGT GGCAAAGAAG AAAATGATGC TGACGCATCT AGTGGTGCGG 
CCTGGGCTGG GCTCCAAGAC TGGATCTATG TCCAAACAGG AGCTTGATGA TATCCTCAAA 
TTTGGCACTG AGGAACTATT CAAGGATGAA GCCACTGATG GAGGAGGAGA CAACAAAGAG 
GGAGAAGATA GCAGTGTTAT CCACTACGAT GATAAGGCCA TTGAACGGCT GCTAGACCGT 
AACCAGGATG AGACTGAAGA CACAGAATTG CAGGGCATGA ATGAATATTT GAGCTCATTC 
AAAGTGGCCC AGTATGTGGT ACGGGAAGAA GAAATGGGGG AAGAGGAGGT AGAACGGGAA 
ATCATTAAAC AGGAAGAAAG TGTGGATCCT GACTACTGGG AGAAATTGCT GCGGCACCAT 
TATGAGCAGC AGCAAGAAGA TCTAGCCCGA AATCTGGGCA AAGGAAAAAG AATCCGTAAA 
CAGGTCAACT ACAATGATGG CTCCCAGGAG GACCGAGATT GGCAGGACGA CCAGTCCGAC 
AACCAGTCCG ATTACTCAGT GGCTTCAGAG GAAGGTGATG AAGACTTTGA TGAACGTTCA 
GAAGCTCCCC GTAGGCCCAG TCGTAAGGGC CTGCGGAATG ATAAAGATAA GCCATTGCCT 
CCTCTGTTGG CCCGTGTTGG TGGGAATATT GAAGTACTTG GTTTTAATGC TCGTCAGCGA 
AAAGCCTTTC TTAATGCAAT TATGCGATAT GGTATGCCAC CTCAGGATGC TTTTACTACC 
CAGTGGCTTG TAAGAGACCT GCGAGGCAAA TCAGAGAAAG AGTTCAAGGC ATATGTCTCT 
CTTTTCATGC GGCATTTATG TGAGCCGGGG GCAGATGGGG CTGAGACCTT TGCTGATGGT 
GTCCCCCGAG AAGGCCTGTC TCGCCAGCAT GTCCTTACTA GAATTGGTGT TATGTCTTTG 
ATTCGCAAGA AGGTTCAGGA GTTTGAACAT GTTAATGGGC GCTGGAGCAT GCCTGAACTG 
GCTGAGGTGG AGGAAAACAA GAAGATGTCC CAGCCAGGGT CACCCTCCCC AAAAACTCCT 
ACACCCTCCA CTCCAGGGGA CACGCAGCCC AACACTCCTG CACCTGTCCC ACCTGCTGAA 
GATGGGATAA AAATAGAGGA AAATAGCCTC AAAGAAGAAG AGAGCATAGA AGGAGAAAAG 
GAGGTTAAAT CTACAGCCCC TGAGACTGCC ATTGAGTGTA CACAGGCCCC TGCCCCTGCC 
TCAGAGGATG AAAAGGTCGT TGTTGAACCC CCTGAGGGAG AGGAGAAAGT GGAAAAGGCA 
GAGGTGAAGG AGAGAACAGA GGAACCTATG GAGACAGAGC CCAAAGGTAA AGGTGCTGCT 
GATGTAGAGA AGGTGGAGGA AAAGTCAGCA ATAGATCTGA CCCCTATTGT GGTAGAAGAC 
AAAGAAGAGA AGAAAGAAGA AGAAGAGAAA AAAGAGGTGA TGCTTCAGAA TGGAGAGACC 
CCCAAGGACC TGAATGATGA GAAACAGAAG AAAAATATTA AACAACGTTT CATGTTTAAC 
ATTGCAGATG GTGGTTTTAC TGAGTTGCAC TCCCTTTGGC AGAATGAAGA GCGGGCAGCC 
ACAGTTACCA AGAAGACTTA TGAGATCTGG CATCGACGGC ATGACTACTG GCTGCTAGCC 
GGCATTATAA ACCATGGCTA TGCCCGGTGG CAAGACATCC AGAATGACCC ACGCTATGCC 
ATCCTCAATG AGCCTTTCAA GGGTGAAATG AACCGTGGCA ATTTCTTAGA GATCAAGAAT 
AAATTTCTAG CTCGAAGGTT TAAGCTCTTA GAACAAGCTC TGGTGATTGA GGAACAGCTG 
CGCCGGGCTG CTTACTTGAA CATGTCAGAA GACCCTTCTC ACCCTTCCAT GGCCCTCAAC 
ACCCGCTTTG CTGAGGTGGA GTGTTTGGCG GAAAGTCATC AGCACCTGTC CAAGGAGTCA 
ATGGCAGGAA ACAAGCCAGC CAATGCAGTC CTGCACAAAG GTATTCTGAA ACAGCTGGAA 
GAACTGCTGA GTGACATGAA AGCTGATGTG ACTCGACTCC CAGCTACCAT TGCCCGAATT 
CCCCCAGTTG CTGTGAGGTT ACAGATGTCA GAGCGTAACA TTCTCAGCCG CCTGGCAAAC 
CGGGCACCCG AACCTACCCC ACAGCAGGTA GCCCAGCAGC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006719023.1
CDS	219..5963
Translation	MASGLGSPSPCSAGSEEEDMDALLNNSLPPPHPENEEDPEEDLSETETPKLKKKKKPKKPRDPKIPKSKRQKKERMLLCRQLGDSSGEGPEFVEEEEEVALRSDSEGSDYTPGKKKKKKLGPKKEKKSKSKRKEEEEEEDDDDDSKEPKSSAQLLEDWGMEDIDHVFSEEDYRTLTNYKAFSQFVRPLIAAKNPKIAVSKMMMVLGAKWREFSTNNPFKGSSGASVAAAAAAAVAVVESMVTATEVAPPPPPVEVPIRKAKTKEGKGPNARRKPKGSPRVPDAKKPKPKKVAPLKIKLGGFGSKRKRSSSEDDDLDVESDFDDASINSYSVSDGSTSRSSRSRKKLRTTKKKKKGEEEVTAVDGYETDHQDYCEVCQQGGEIILCDTCPRAYHMVCLDPDMEKAPEGKWSCPHCEKEGIQWEAKEDNSEGEEILEEVGGDLEEEDDHHMEFCRVCKDGGELLCCDTCPSSYHIHCLNPPLPEIPNGEWLCPRCTCPALKGKVQKILIWKWGQPPSPTPVPRPPDADPNTPSPKPLEGRPERQFFVKWQGMSYWHCSWVSELQLELHCQVMFRNYQRKNDMDEPPSGDFGGDEEKSRKRKNKDPKFAEMEERFYRYGIKPEWMMIHRILNHSVDKKGHVHYLIKWRDLPYDQASWESEDVEIQDYDLFKQSYWNHRELMRGEEGRPGKKLKKVKLRKLERPPETPTVDPTVKYERQPEYLDATGGTLHPYQMEGLNWLRFSWAQGTDTILADEMGLGKTVQTAVFLYSLYKEGHSKGPFLVSAPLSTIINWEREFEMWAPDMYVVTYVGDKDSRAIIRENEFSFEDNAIRGGKKASRMKKEASVKFHVLLTSYELITIDMAILGSIDWACLIVDEAHRLKNNQSKFFRVLNGYSLQHKLLLTGTPLQNNLEELFHLLNFLTPERFHNLEGFLEEFADIAKEDQIKKLHDMLGPHMLRRLKADVFKNMPSKTELIVRVELSPMQKKYYKYILTRNFEALNARGGGNQVSLLNVVMDLKKCCNHPYLFPVAAMEAPKMPNGMYDGSALIRASGKLLLLQKMLKNLKEGGHRVLIFSQMTKMLDLLEDFLEHEGYKYERIDGGITGNMRQEAIDRFNAPGAQQFCFLLSTRAGGLGINLATADTVIIYDSDWNPHNDIQAFSRAHRIGQNKKVMIYRFVTRASVEERITQVAKKKMMLTHLVVRPGLGSKTGSMSKQELDDILKFGTEELFKDEATDGGGDNKEGEDSSVIHYDDKAIERLLDRNQDETEDTELQGMNEYLSSFKVAQYVVREEEMGEEEVEREIIKQEESVDPDYWEKLLRHHYEQQQEDLARNLGKGKRIRKQVNYNDGSQEDRDWQDDQSDNQSDYSVASEEGDEDFDERSEAPRRPSRKGLRNDKDKPLPPLLARVGGNIEVLGFNARQRKAFLNAIMRYGMPPQDAFTTQWLVRDLRGKSEKEFKAYVSLFMRHLCEPGADGAETFADGVPREGLSRQHVLTRIGVMSLIRKKVQEFEHVNGRWSMPELAEVEENKKMSQPGSPSPKTPTPSTPGDTQPNTPAPVPPAEDGIKIEENSLKEEESIEGEKEVKSTAPETAIECTQAPAPASEDEKVVVEPPEGEEKVEKAEVKERTEEPMETEPKGKGAADVEKVEEKSAIDLTPIVVEDKEEKKEEEEKKEVMLQNGETPKDLNDEKQKKNIKQRFMFNIADGGFTELHSLWQNEERAATVTKKTYEIWHRRHDYWLLAGIINHGYARWQDIQNDPRYAILNEPFKGEMNRGNFLEIKNKFLARRFKLLEQALVIEEQLRRAAYLNMSEDPSHPSMALNTRFAEVECLAESHQHLSKESMAGNKPANAVLHKGILKQLEELLSDMKADVTRLPATIARIPPVAVRLQMSERNILSRLANRAPEPTPQQVAQQQ

Target ORF information:

RefSeq Version	XM_006718960.1
Organism	Homo sapiens(human)
Definition	Homo sapiens chromodomain helicase DNA binding protein 4 (CHD4), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006718960.1

ORF Insert Sequence:

ATGGCGTCGG GCCTGGGCTC CCCGTCCCCC TGCTCGGCGG GCAGTGAGGA GGAGGATATG 
GATGCACTTT TGAACAACAG CCTGCCCCCA CCCCACCCAG AAAATGAAGA GGACCCAGAA 
GAGGATTTGT CAGAAACAGA GACTCCAAAG CTCAAGAAGA AGAAAAAGCC TAAGAAACCT 
CGGGACCCTA AAATCCCTAA GAGCAAGCGC CAAAAAAAGG AGCGTATGCT CTTATGCCGG 
CAGCTGGGGG ACAGCTCTGG GGAGGGGCCA GAGTTTGTGG AGGAGGAGGA AGAGGTGGCT 
CTGCGCTCAG ACAGTGAGGG CAGCGACTAT ACTCCTGGCA AGAAGAAGAA GAAGAAGCTT 
GGACCTAAGA AAGAGAAGAA GAGCAAATCC AAGCGGAAGG AGGAGGAGGA GGAGGAGGAT 
GATGATGATG ATTCAAAGGA GCCTAAATCA TCTGCTCAGC TCCTGGAAGA CTGGGGCATG 
GAAGACATTG ACCACGTGTT CTCAGAGGAG GATTATCGAA CCCTCACCAA CTACAAGGCC 
TTCAGCCAGT TTGTCAGACC CCTCATTGCT GCCAAAAATC CCAAGATTGC TGTCTCCAAG 
ATGATGATGG TTTTGGGTGC AAAATGGCGG GAGTTCAGTA CCAATAACCC CTTCAAAGGC 
AGTTCTGGGG CATCAGTGGC AGCTGCGGCA GCAGCAGCGG TAGCTGTGGT GGAGAGCATG 
GTGACAGCCA CTGAGGTTGC ACCACCACCT CCCCCTGTGG AGGTGCCTAT CCGCAAGGCC 
AAGACCAAGG AGGGCAAAGG TCCCAATGCT CGGAGGAAGC CCAAGGGCAG CCCTCGTGTA 
CCTGATGCCA AGAAGCCTAA ACCCAAGAAA GTAGCTCCCC TGAAAATCAA GCTGGGAGGT 
TTTGGTTCCA AGCGTAAGAG ATCCTCGAGT GAGGATGATG ACTTAGATGT GGAATCTGAC 
TTCGATGATG CCAGTATCAA TAGCTATTCT GTTTCTGATG GTTCCACCAG CCGTAGTAGC 
CGCAGCCGCA AGAAACTCCG AACCACTAAA AAGAAAAAGA AAGGCGAGGA GGAGGTGACT 
GCTGTGGATG GTTATGAGAC AGACCACCAG GACTATTGCG AGGTGTGCCA GCAAGGCGGT 
GAGATCATCC TGTGTGATAC CTGTCCCCGT GCTTACCACA TGGTCTGCCT GGATCCCGAC 
ATGGAGAAGG CTCCCGAGGG CAAGTGGAGC TGCCCACACT GCGAGAAGGA AGGCATCCAG 
TGGGAAGCTA AAGAGGACAA TTCGGAGGGT GAGGAGATCC TGGAAGAGGT TGGGGGAGAC 
CTCGAAGAGG AGGATGACCA CCATATGGAA TTCTGTCGGG TCTGCAAGGA TGGTGGGGAA 
CTGCTCTGCT GTGATACCTG TCCTTCTTCC TACCACATCC ACTGCCTGAA TCCCCCACTT 
CCAGAGATCC CCAACGGTGA ATGGCTCTGT CCCCGTTGTA CGTGTCCAGC TCTGAAGGGC 
AAAGTGCAGA AGATCCTAAT CTGGAAGTGG GGTCAGCCAC CATCTCCCAC ACCAGTGCCT 
CGGCCTCCAG ATGCTGATCC CAACACGCCC TCCCCAAAGC CCTTGGAGGG GCGGCCAGAG 
CGGCAGTTCT TTGTGAAATG GCAAGGCATG TCTTACTGGC ACTGCTCCTG GGTTTCTGAA 
CTGCAGCTGG AGCTGCACTG TCAGGTGATG TTCCGAAACT ATCAGCGGAA GAATGATATG 
GATGAGCCAC CTTCTGGGGA CTTTGGTGGT GATGAAGAGA AAAGCCGAAA GCGAAAGAAC 
AAGGACCCTA AATTTGCAGA GATGGAGGAA CGCTTCTATC GCTATGGGAT AAAACCCGAG 
TGGATGATGA TCCACCGAAT CCTCAACCAC AGTGTGGACA AGAAGGGCCA CGTCCACTAC 
TTGATCAAGT GGCGGGACTT ACCTTACGAT CAGGCTTCTT GGGAGAGTGA GGATGTGGAG 
ATCCAGGATT ACGACCTGTT CAAGCAGAGC TATTGGAATC ACAGGGAGTT AATGAGGGGT 
GAGGAAGGCC GACCAGGCAA GAAGCTCAAG AAGGTGAAGC TTCGGAAGTT GGAGAGGCCT 
CCAGAAACGC CAACAGTTGA TCCAACAGTG AAGTATGAGC GACAGCCAGA GTACCTGGAT 
GCTACAGGTG GAACCCTGCA CCCCTATCAA ATGGAGGGCC TGAATTGGTT GCGCTTCTCC 
TGGGCTCAGG GCACTGACAC CATCTTGGCT GATGAGATGG GCCTTGGGAA AACTGTACAG 
ACAGCAGTCT TCCTGTATTC CCTTTACAAG GAGGGTCATT CCAAAGGCCC CTTCCTAGTG 
AGCGCCCCTC TTTCTACCAT CATCAACTGG GAGCGGGAGT TTGAAATGTG GGCTCCAGAC 
ATGTATGTCG TAACCTATGT GGGTGACAAG GACAGCCGTG CCATCATCCG AGAGAATGAG 
TTCTCCTTTG AAGACAATGC CATTCGTGGT GGCAAGAAGG CCTCCCGCAT GAAGAAAGAG 
GCATCTGTGA AATTCCATGT GCTGCTGACA TCCTATGAAT TGATCACCAT TGACATGGCT 
ATTTTGGGCT CTATTGATTG GGCCTGCCTC ATCGTGGATG AAGCCCATCG GCTGAAGAAC 
AATCAGTCTA AGTTCTTCCG GGTATTGAAT GGTTACTCAC TCCAGCACAA GCTGTTGCTG 
ACTGGGACAC CATTACAAAA CAATCTGGAA GAGTTGTTTC ATCTGCTCAA CTTTCTCACC 
CCCGAGAGGT TCCACAATTT GGAAGGTTTT TTGGAGGAGT TTGCTGACAT TGCCAAGGAG 
GACCAGATAA AAAAACTGCA TGACATGCTG GGGCCGCACA TGTTGCGGCG GCTCAAAGCC 
GATGTGTTCA AGAACATGCC CTCCAAGACA GAACTAATTG TGCGTGTGGA GCTGAGCCCT 
ATGCAGAAGA AATACTACAA GTACATCCTC ACTCGAAATT TTGAAGCACT CAATGCCCGA 
GGTGGTGGCA ACCAGGTGTC TCTGCTGAAT GTGGTGATGG ATCTTAAGAA GTGCTGCAAC 
CATCCATACC TCTTCCCTGT GGCTGCAATG GAAGCTCCTA AGATGCCTAA TGGCATGTAT 
GATGGCAGTG CCCTAATCAG AGCATCTGGG AAATTATTGC TGCTGCAGAA AATGCTCAAG 
AACCTTAAGG AGGGTGGGCA TCGTGTACTC ATCTTTTCCC AGATGACCAA GATGCTAGAC 
CTGCTAGAGG ATTTCTTGGA ACATGAAGGT TATAAATACG AACGCATCGA TGGTGGAATC 
ACTGGGAACA TGCGGCAAGA GGCCATTGAC CGCTTCAATG CACCGGGTGC TCAGCAGTTC 
TGCTTCTTGC TTTCCACTCG AGCTGGGGGC CTTGGAATCA ATCTGGCCAC TGCTGACACA 
GTTATTATCT ATGACTCTGA CTGGAACCCC CATAATGACA TTCAGGCCTT TAGCAGAGCT 
CACCGGATTG GGCAAAATAA AAAGGTAATG ATCTACCGGT TTGTGACCCG TGCGTCAGTG 
GAGGAGCGCA TCACGCAGGT GGCAAAGAAG AAAATGATGC TGACGCATCT AGTGGTGCGG 
CCTGGGCTGG GCTCCAAGAC TGGATCTATG TCCAAACAGG AGCTTGATGA TATCCTCAAA 
TTTGGCACTG AGGAACTATT CAAGGATGAA GCCACTGATG GAGGAGGAGA CAACAAAGAG 
GGAGAAGATA GCAGTGTTAT CCACTACGAT GATAAGGCCA TTGAACGGCT GCTAGACCGT 
AACCAGGATG AGACTGAAGA CACAGAATTG CAGGGCATGA ATGAATATTT GAGCTCATTC 
AAAGTGGCCC AGTATGTGGT ACGGGAAGAA GAAATGGGGG AAGAGGAGGT AGAACGGGAA 
ATCATTAAAC AGGAAGAAAG TGTGGATCCT GACTACTGGG AGAAATTGCT GCGGCACCAT 
TATGAGCAGC AGCAAGAAGA TCTAGCCCGA AATCTGGGCA AAGGAAAAAG AATCCGTAAA 
CAGGTCAACT ACAATGATGG CTCCCAGGAG GACCGAGATT GGCAGGACGA CCAGTCCGAC 
AACCAGTCCG ATTACTCAGT GGCTTCAGAG GAAGGTGATG AAGACTTTGA TGAACGTTCA 
GAAGCTCCCC GTAGGCCCAG TCGTAAGGGC CTGCGGAATG ATAAAGATAA GCCATTGCCT 
CCTCTGTTGG CCCGTGTTGG TGGGAATATT GAAGTACTTG GTTTTAATGC TCGTCAGCGA 
AAAGCCTTTC TTAATGCAAT TATGCGATAT GGTATGCCAC CTCAGGATGC TTTTACTACC 
CAGTGGCTTG TAAGAGACCT GCGAGGCAAA TCAGAGAAAG AGTTCAAGGC ATATGTCTCT 
CTTTTCATGC GGCATTTATG TGAGCCGGGG GCAGATGGGG CTGAGACCTT TGCTGATGGT 
GTCCCCCGAG AAGGCCTGTC TCGCCAGCAT GTCCTTACTA GAATTGGTGT TATGTCTTTG 
ATTCGCAAGA AGGTTCAGGA GTTTGAACAT GTTAATGGGC GCTGGAGCAT GCCTGAACTG 
GCTGAGGTGG AGGAAAACAA GAAGATGTCC CAGCCAGGGT CACCCTCCCC AAAAACTCCT 
ACACCCTCCA CTCCAGGGGA CACGCAGCCC AACACTCCTG CACCTGTCCC ACCTGCTGAA 
GATGGGATAA AAATAGAGGA AAATAGCCTC AAAGAAGAAG AGAGCATAGA AGGAGAAAAG 
GAGGTTAAAT CTACAGCCCC TGAGACTGCC ATTGAGTGTA CACAGGCCCC TGCCCCTGCC 
TCAGAGGATG AAAAGGTCGT TGTTGAACCC CCTGAGGGAG AGGAGAAAGT GGAAAAGGCA 
GAGGTGAAGG AGAGAACAGA GGAACCTATG GAGACAGAGC CCAAAGGTAA AGGTGCTGCT 
GATGTAGAGA AGGTGGAGGA AAAGTCAGCA ATAGATCTGA CCCCTATTGT GGTAGAAGAC 
AAAGAAGAGA AGAAAGAAGA AGAAGAGAAA AAAGAGGTGA TGCTTCAGAA TGGAGAGACC 
CCCAAGGACC TGAATGATGA GAAACAGAAG AAAAATATTA AACAACGTTT CATGTTTAAC 
ATTGCAGATG GTGGTTTTAC TGAGTTGCAC TCCCTTTGGC AGAATGAAGA GCGGGCAGCC 
ACAGTTACCA AGAAGACTTA TGAGATCTGG CATCGACGGC ATGACTACTG GCTGCTAGCC 
GGCATTATAA ACCATGGCTA TGCCCGGTGG CAAGACATCC AGAATGACCC ACGCTATGCC 
ATCCTCAATG AGCCTTTCAA GGGTGAAATG AACCGTGGCA ATTTCTTAGA GATCAAGAAT 
AAATTTCTAG CTCGAAGGTT TAAGCTCTTA GAACAAGCTC TGGTGATTGA GGAACAGCTG 
CGCCGGGCTG CTTACTTGAA CATGTCAGAA GACCCTTCTC ACCCTTCCAT GGCCCTCAAC 
ACCCGCTTTG CTGAGGTGGA GTGTTTGGCG GAAAGTCATC AGCACCTGTC CAAGGAGTCA 
ATGGCAGGAA ACAAGCCAGC CAATGCAGTC CTGCACAAAG GTATTCTGAA ACAGCTGGAA 
GAACTGCTGA GTGACATGAA AGCTGATGTG ACTCGACTCC CAGCTACCAT TGCCCGAATT 
CCCCCAGTTG CTGTGAGGTT ACAGATGTCA GAGCGTAACA TTCTCAGCCG CCTGGCAAAC 
CGGGCACCCG AACCTACCCC ACAGCAGGTA GCCCAGCAGC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHD4 ( XM_006718961.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_006719024.1 Homo sapiens chromodomain helicase DNA binding protein 4 (CHD4), transcript variant X12, mRNA.

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CloneID	OHu45953
Clone ID Related Accession (Same CDS sequence)	XM_006718961.2
Accession Version	XM_006718961.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5727bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-25
Organism	Homo sapiens(human)
Product	chromodomain-helicase-DNA-binding protein 4 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_006718961.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCGG GCCTGGGCTC CCCGTCCCCC TGCTCGGCGG GCAGTGAGGA GGAGGATATG 
GATGCACTTT TGAACAACAG CCTGCCCCCA CCCCACCCAG AAAATGAAGA GGACCCAGAA 
GAGGATTTGT CAGAAACAGA GACTCCAAAG CTCAAGAAGA AGAAAAAGCC TAAGAAACCT 
CGGGACCCTA AAATCCCTAA GAGCAAGCGC CAAAAAAAGG AGCTGGGGGA CAGCTCTGGG 
GAGGGGCCAG AGTTTGTGGA GGAGGAGGAA GAGGTGGCTC TGCGCTCAGA CAGTGAGGGC 
AGCGACTATA CTCCTGGCAA GAAGAAGAAG AAGAAGCTTG GACCTAAGAA AGAGAAGAAG 
AGCAAATCCA AGCGGAAGGA GGAGGAGGAG GAGGAGGATG ATGATGATGA TTCAAAGGAG 
CCTAAATCAT CTGCTCAGCT CCTGGAAGAC TGGGGCATGG AAGACATTGA CCACGTGTTC 
TCAGAGGAGG ATTATCGAAC CCTCACCAAC TACAAGGCCT TCAGCCAGTT TGTCAGACCC 
CTCATTGCTG CCAAAAATCC CAAGATTGCT GTCTCCAAGA TGATGATGGT TTTGGGTGCA 
AAATGGCGGG AGTTCAGTAC CAATAACCCC TTCAAAGGCA GTTCTGGGGC ATCAGTGGCA 
GCTGCGGCAG CAGCAGCGGT AGCTGTGGTG GAGAGCATGG TGACAGCCAC TGAGGTTGCA 
CCACCACCTC CCCCTGTGGA GGTGCCTATC CGCAAGGCCA AGACCAAGGA GGGCAAAGGT 
CCCAATGCTC GGAGGAAGCC CAAGGGCAGC CCTCGTGTAC CTGATGCCAA GAAGCCTAAA 
CCCAAGAAAG TAGCTCCCCT GAAAATCAAG CTGGGAGGTT TTGGTTCCAA GCGTAAGAGA 
TCCTCGAGTG AGGATGATGA CTTAGATGTG GAATCTGACT TCGATGATGC CAGTATCAAT 
AGCTATTCTG TTTCTGATGG TTCCACCAGC CGTAGTAGCC GCAGCCGCAA GAAACTCCGA 
ACCACTAAAA AGAAAAAGAA AGGCGAGGAG GAGGTGACTG CTGTGGATGG TTATGAGACA 
GACCACCAGG ACTATTGCGA GGTGTGCCAG CAAGGCGGTG AGATCATCCT GTGTGATACC 
TGTCCCCGTG CTTACCACAT GGTCTGCCTG GATCCCGACA TGGAGAAGGC TCCCGAGGGC 
AAGTGGAGCT GCCCACACTG CGAGAAGGAA GGCATCCAGT GGGAAGCTAA AGAGGACAAT 
TCGGAGGGTG AGGAGATCCT GGAAGAGGTT GGGGGAGACC TCGAAGAGGA GGATGACCAC 
CATATGGAAT TCTGTCGGGT CTGCAAGGAT GGTGGGGAAC TGCTCTGCTG TGATACCTGT 
CCTTCTTCCT ACCACATCCA CTGCCTGAAT CCCCCACTTC CAGAGATCCC CAACGGTGAA 
TGGCTCTGTC CCCGTTGTAC GTGTCCAGCT CTGAAGGGCA AAGTGCAGAA GATCCTAATC 
TGGAAGTGGG GTCAGCCACC ATCTCCCACA CCAGTGCCTC GGCCTCCAGA TGCTGATCCC 
AACACGCCCT CCCCAAAGCC CTTGGAGGGG CGGCCAGAGC GGCAGTTCTT TGTGAAATGG 
CAAGGCATGT CTTACTGGCA CTGCTCCTGG GTTTCTGAAC TGCAGCTGGA GCTGCACTGT 
CAGGTGATGT TCCGAAACTA TCAGCGGAAG AATGATATGG ATGAGCCACC TTCTGGGGAC 
TTTGGTGGTG ATGAAGAGAA AAGCCGAAAG CGAAAGAACA AGGACCCTAA ATTTGCAGAG 
ATGGAGGAAC GCTTCTATCG CTATGGGATA AAACCCGAGT GGATGATGAT CCACCGAATC 
CTCAACCACA GTGTGGACAA GAAGGGCCAC GTCCACTACT TGATCAAGTG GCGGGACTTA 
CCTTACGATC AGGCTTCTTG GGAGAGTGAG GATGTGGAGA TCCAGGATTA CGACCTGTTC 
AAGCAGAGCT ATTGGAATCA CAGGGAGTTA ATGAGGGGTG AGGAAGGCCG ACCAGGCAAG 
AAGCTCAAGA AGGTGAAGCT TCGGAAGTTG GAGAGGCCTC CAGAAACGCC AACAGTTGAT 
CCAACAGTGA AGTATGAGCG ACAGCCAGAG TACCTGGATG CTACAGGTGG AACCCTGCAC 
CCCTATCAAA TGGAGGGCCT GAATTGGTTG CGCTTCTCCT GGGCTCAGGG CACTGACACC 
ATCTTGGCTG ATGAGATGGG CCTTGGGAAA ACTGTACAGA CAGCAGTCTT CCTGTATTCC 
CTTTACAAGG AGGGTCATTC CAAAGGCCCC TTCCTAGTGA GCGCCCCTCT TTCTACCATC 
ATCAACTGGG AGCGGGAGTT TGAAATGTGG GCTCCAGACA TGTATGTCGT AACCTATGTG 
GGTGACAAGG ACAGCCGTGC CATCATCCGA GAGAATGAGT TCTCCTTTGA AGACAATGCC 
ATTCGTGGTG GCAAGAAGGC CTCCCGCATG AAGAAAGAGG CATCTGTGAA ATTCCATGTG 
CTGCTGACAT CCTATGAATT GATCACCATT GACATGGCTA TTTTGGGCTC TATTGATTGG 
GCCTGCCTCA TCGTGGATGA AGCCCATCGG CTGAAGAACA ATCAGTCTAA GTTCTTCCGG 
GTATTGAATG GTTACTCACT CCAGCACAAG CTGTTGCTGA CTGGGACACC ATTACAAAAC 
AATCTGGAAG AGTTGTTTCA TCTGCTCAAC TTTCTCACCC CCGAGAGGTT CCACAATTTG 
GAAGGTTTTT TGGAGGAGTT TGCTGACATT GCCAAGGAGG ACCAGATAAA AAAACTGCAT 
GACATGCTGG GGCCGCACAT GTTGCGGCGG CTCAAAGCCG ATGTGTTCAA GAACATGCCC 
TCCAAGACAG AACTAATTGT GCGTGTGGAG CTGAGCCCTA TGCAGAAGAA ATACTACAAG 
TACATCCTCA CTCGAAATTT TGAAGCACTC AATGCCCGAG GTGGTGGCAA CCAGGTGTCT 
CTGCTGAATG TGGTGATGGA TCTTAAGAAG TGCTGCAACC ATCCATACCT CTTCCCTGTG 
GCTGCAATGG AAGCTCCTAA GATGCCTAAT GGCATGTATG ATGGCAGTGC CCTAATCAGA 
GCATCTGGGA AATTATTGCT GCTGCAGAAA ATGCTCAAGA ACCTTAAGGA GGGTGGGCAT 
CGTGTACTCA TCTTTTCCCA GATGACCAAG ATGCTAGACC TGCTAGAGGA TTTCTTGGAA 
CATGAAGGTT ATAAATACGA ACGCATCGAT GGTGGAATCA CTGGGAACAT GCGGCAAGAG 
GCCATTGACC GCTTCAATGC ACCGGGTGCT CAGCAGTTCT GCTTCTTGCT TTCCACTCGA 
GCTGGGGGCC TTGGAATCAA TCTGGCCACT GCTGACACAG TTATTATCTA TGACTCTGAC 
TGGAACCCCC ATAATGACAT TCAGGCCTTT AGCAGAGCTC ACCGGATTGG GCAAAATAAA 
AAGGTAATGA TCTACCGGTT TGTGACCCGT GCGTCAGTGG AGGAGCGCAT CACGCAGGTG 
GCAAAGAAGA AAATGATGCT GACGCATCTA GTGGTGCGGC CTGGGCTGGG CTCCAAGACT 
GGATCTATGT CCAAACAGGA GCTTGATGAT ATCCTCAAAT TTGGCACTGA GGAACTATTC 
AAGGATGAAG CCACTGATGG AGGAGGAGAC AACAAAGAGG GAGAAGATAG CAGTGTTATC 
CACTACGATG ATAAGGCCAT TGAACGGCTG CTAGACCGTA ACCAGGATGA GACTGAAGAC 
ACAGAATTGC AGGGCATGAA TGAATATTTG AGCTCATTCA AAGTGGCCCA GTATGTGGTA 
CGGGAAGAAG AAATGGGGGA GGAAGAGGAG GTAGAACGGG AAATCATTAA ACAGGAAGAA 
AGTGTGGATC CTGACTACTG GGAGAAATTG CTGCGGCACC ATTATGAGCA GCAGCAAGAA 
GATCTAGCCC GAAATCTGGG CAAAGGAAAA AGAATCCGTA AACAGGTCAA CTACAATGAT 
GGCTCCCAGG AGGACCGAGA TTGGCAGGAC GACCAGTCCG ACAACCAGTC CGATTACTCA 
GTGGCTTCAG AGGAAGGTGA TGAAGACTTT GATGAACGTT CAGAAGCTCC CCGTAGGCCC 
AGTCGTAAGG GCCTGCGGAA TGATAAAGAT AAGCCATTGC CTCCTCTGTT GGCCCGTGTT 
GGTGGGAATA TTGAAGTACT TGGTTTTAAT GCTCGTCAGC GAAAAGCCTT TCTTAATGCA 
ATTATGCGAT ATGGTATGCC ACCTCAGGAT GCTTTTACTA CCCAGTGGCT TGTAAGAGAC 
CTGCGAGGCA AATCAGAGAA AGAGTTCAAG GCATATGTCT CTCTTTTCAT GCGGCATTTA 
TGTGAGCCGG GGGCAGATGG GGCTGAGACC TTTGCTGATG GTGTCCCCCG AGAAGGCCTG 
TCTCGCCAGC ATGTCCTTAC TAGAATTGGT GTTATGTCTT TGATTCGCAA GAAGGTTCAG 
GAGTTTGAAC ATGTTAATGG GCGCTGGAGC ATGCCTGAAC TGGCTGAGGT GGAGGAAAAC 
AAGAAGATGT CCCAGCCAGG GTCACCCTCC CCAAAAACTC CTACACCCTC CACTCCAGGG 
GACACGCAGC CCAACACTCC TGCACCTGTC CCACCTGCTG AAGATGGGAT AAAAATAGAG 
GAAAATAGCC TCAAAGAAGA AGAGAGCATA GAAGGAGAAA AGGAGGTTAA ATCTACAGCC 
CCTGAGACTG CCATTGAGTG TACACAGGCC CCTGCCCCTG CCTCAGAGGA TGAAAAGGTC 
GTTGTTGAAC CCCCTGAGGG AGAGGAGAAA GTGGAAAAGG CAGAGGTGAA GGAGAGAACA 
GAGGAACCTA TGGAGACAGA GCCCAAAGGT AAAGGTGCTG CTGATGTAGA GAAGGTGGAG 
GAAAAGTCAG CAATAGATCT GACCCCTATT GTGGTAGAAG ACAAAGAAGA GAAGAAAGAA 
GAAGAAGAGA AAAAAGAGGT GATGCTTCAG AATGGAGAGA CCCCCAAGGA CCTGAATGAT 
GAGAAACAGA AGAAAAATAT TAAACAACGT TTCATGTTTA ACATTGCAGA TGGTGGTTTT 
ACTGAGTTGC ACTCCCTTTG GCAGAATGAA GAGCGGGCAG CCACAGTTAC CAAGAAGACT 
TATGAGATCT GGCATCGACG GCATGACTAC TGGCTGCTAG CCGGCATTAT AAACCATGGC 
TATGCCCGGT GGCAAGACAT CCAGAATGAC CCACGCTATG CCATCCTCAA TGAGCCTTTC 
AAGGGTGAAA TGAACCGTGG CAATTTCTTA GAGATCAAGA ATAAATTTCT AGCTCGAAGG 
TTTAAGCTCT TAGAACAAGC TCTGGTGATT GAGGAACAGC TGCGCCGGGC TGCTTACTTG 
AACATGTCAG AAGACCCTTC TCACCCTTCC ATGGCCCTCA ACACCCGCTT TGCTGAGGTG 
GAGTGTTTGG CGGAAAGTCA TCAGCACCTG TCCAAGGAGT CAATGGCAGG AAACAAGCCA 
GCCAATGCAG TCCTGCACAA AGGTATTCTG AAACAGCTGG AAGAACTGCT GAGTGACATG 
AAAGCTGATG TGACTCGACT CCCAGCTACC ATTGCCCGAA TTCCCCCAGT TGCTGTGAGG 
TTACAGATGT CAGAGCGTAA CATTCTCAGC CGCCTGGCAA ACCGGGCACC CGAACCTACC 
CCACAGCAGG TAGCCCAGCA GCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006719024.1
CDS	165..5891
Translation	MASGLGSPSPCSAGSEEEDMDALLNNSLPPPHPENEEDPEEDLSETETPKLKKKKKPKKPRDPKIPKSKRQKKELGDSSGEGPEFVEEEEEVALRSDSEGSDYTPGKKKKKKLGPKKEKKSKSKRKEEEEEEDDDDDSKEPKSSAQLLEDWGMEDIDHVFSEEDYRTLTNYKAFSQFVRPLIAAKNPKIAVSKMMMVLGAKWREFSTNNPFKGSSGASVAAAAAAAVAVVESMVTATEVAPPPPPVEVPIRKAKTKEGKGPNARRKPKGSPRVPDAKKPKPKKVAPLKIKLGGFGSKRKRSSSEDDDLDVESDFDDASINSYSVSDGSTSRSSRSRKKLRTTKKKKKGEEEVTAVDGYETDHQDYCEVCQQGGEIILCDTCPRAYHMVCLDPDMEKAPEGKWSCPHCEKEGIQWEAKEDNSEGEEILEEVGGDLEEEDDHHMEFCRVCKDGGELLCCDTCPSSYHIHCLNPPLPEIPNGEWLCPRCTCPALKGKVQKILIWKWGQPPSPTPVPRPPDADPNTPSPKPLEGRPERQFFVKWQGMSYWHCSWVSELQLELHCQVMFRNYQRKNDMDEPPSGDFGGDEEKSRKRKNKDPKFAEMEERFYRYGIKPEWMMIHRILNHSVDKKGHVHYLIKWRDLPYDQASWESEDVEIQDYDLFKQSYWNHRELMRGEEGRPGKKLKKVKLRKLERPPETPTVDPTVKYERQPEYLDATGGTLHPYQMEGLNWLRFSWAQGTDTILADEMGLGKTVQTAVFLYSLYKEGHSKGPFLVSAPLSTIINWEREFEMWAPDMYVVTYVGDKDSRAIIRENEFSFEDNAIRGGKKASRMKKEASVKFHVLLTSYELITIDMAILGSIDWACLIVDEAHRLKNNQSKFFRVLNGYSLQHKLLLTGTPLQNNLEELFHLLNFLTPERFHNLEGFLEEFADIAKEDQIKKLHDMLGPHMLRRLKADVFKNMPSKTELIVRVELSPMQKKYYKYILTRNFEALNARGGGNQVSLLNVVMDLKKCCNHPYLFPVAAMEAPKMPNGMYDGSALIRASGKLLLLQKMLKNLKEGGHRVLIFSQMTKMLDLLEDFLEHEGYKYERIDGGITGNMRQEAIDRFNAPGAQQFCFLLSTRAGGLGINLATADTVIIYDSDWNPHNDIQAFSRAHRIGQNKKVMIYRFVTRASVEERITQVAKKKMMLTHLVVRPGLGSKTGSMSKQELDDILKFGTEELFKDEATDGGGDNKEGEDSSVIHYDDKAIERLLDRNQDETEDTELQGMNEYLSSFKVAQYVVREEEMGEEEEVEREIIKQEESVDPDYWEKLLRHHYEQQQEDLARNLGKGKRIRKQVNYNDGSQEDRDWQDDQSDNQSDYSVASEEGDEDFDERSEAPRRPSRKGLRNDKDKPLPPLLARVGGNIEVLGFNARQRKAFLNAIMRYGMPPQDAFTTQWLVRDLRGKSEKEFKAYVSLFMRHLCEPGADGAETFADGVPREGLSRQHVLTRIGVMSLIRKKVQEFEHVNGRWSMPELAEVEENKKMSQPGSPSPKTPTPSTPGDTQPNTPAPVPPAEDGIKIEENSLKEEESIEGEKEVKSTAPETAIECTQAPAPASEDEKVVVEPPEGEEKVEKAEVKERTEEPMETEPKGKGAADVEKVEEKSAIDLTPIVVEDKEEKKEEEEKKEVMLQNGETPKDLNDEKQKKNIKQRFMFNIADGGFTELHSLWQNEERAATVTKKTYEIWHRRHDYWLLAGIINHGYARWQDIQNDPRYAILNEPFKGEMNRGNFLEIKNKFLARRFKLLEQALVIEEQLRRAAYLNMSEDPSHPSMALNTRFAEVECLAESHQHLSKESMAGNKPANAVLHKGILKQLEELLSDMKADVTRLPATIARIPPVAVRLQMSERNILSRLANRAPEPTPQQVAQQQ

Target ORF information:

RefSeq Version	XM_006718961.2
Organism	Homo sapiens(human)
Definition	Homo sapiens chromodomain helicase DNA binding protein 4 (CHD4), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006718961.2

ORF Insert Sequence:

ATGGCGTCGG GCCTGGGCTC CCCGTCCCCC TGCTCGGCGG GCAGTGAGGA GGAGGATATG 
GATGCACTTT TGAACAACAG CCTGCCCCCA CCCCACCCAG AAAATGAAGA GGACCCAGAA 
GAGGATTTGT CAGAAACAGA GACTCCAAAG CTCAAGAAGA AGAAAAAGCC TAAGAAACCT 
CGGGACCCTA AAATCCCTAA GAGCAAGCGC CAAAAAAAGG AGCTGGGGGA CAGCTCTGGG 
GAGGGGCCAG AGTTTGTGGA GGAGGAGGAA GAGGTGGCTC TGCGCTCAGA CAGTGAGGGC 
AGCGACTATA CTCCTGGCAA GAAGAAGAAG AAGAAGCTTG GACCTAAGAA AGAGAAGAAG 
AGCAAATCCA AGCGGAAGGA GGAGGAGGAG GAGGAGGATG ATGATGATGA TTCAAAGGAG 
CCTAAATCAT CTGCTCAGCT CCTGGAAGAC TGGGGCATGG AAGACATTGA CCACGTGTTC 
TCAGAGGAGG ATTATCGAAC CCTCACCAAC TACAAGGCCT TCAGCCAGTT TGTCAGACCC 
CTCATTGCTG CCAAAAATCC CAAGATTGCT GTCTCCAAGA TGATGATGGT TTTGGGTGCA 
AAATGGCGGG AGTTCAGTAC CAATAACCCC TTCAAAGGCA GTTCTGGGGC ATCAGTGGCA 
GCTGCGGCAG CAGCAGCGGT AGCTGTGGTG GAGAGCATGG TGACAGCCAC TGAGGTTGCA 
CCACCACCTC CCCCTGTGGA GGTGCCTATC CGCAAGGCCA AGACCAAGGA GGGCAAAGGT 
CCCAATGCTC GGAGGAAGCC CAAGGGCAGC CCTCGTGTAC CTGATGCCAA GAAGCCTAAA 
CCCAAGAAAG TAGCTCCCCT GAAAATCAAG CTGGGAGGTT TTGGTTCCAA GCGTAAGAGA 
TCCTCGAGTG AGGATGATGA CTTAGATGTG GAATCTGACT TCGATGATGC CAGTATCAAT 
AGCTATTCTG TTTCTGATGG TTCCACCAGC CGTAGTAGCC GCAGCCGCAA GAAACTCCGA 
ACCACTAAAA AGAAAAAGAA AGGCGAGGAG GAGGTGACTG CTGTGGATGG TTATGAGACA 
GACCACCAGG ACTATTGCGA GGTGTGCCAG CAAGGCGGTG AGATCATCCT GTGTGATACC 
TGTCCCCGTG CTTACCACAT GGTCTGCCTG GATCCCGACA TGGAGAAGGC TCCCGAGGGC 
AAGTGGAGCT GCCCACACTG CGAGAAGGAA GGCATCCAGT GGGAAGCTAA AGAGGACAAT 
TCGGAGGGTG AGGAGATCCT GGAAGAGGTT GGGGGAGACC TCGAAGAGGA GGATGACCAC 
CATATGGAAT TCTGTCGGGT CTGCAAGGAT GGTGGGGAAC TGCTCTGCTG TGATACCTGT 
CCTTCTTCCT ACCACATCCA CTGCCTGAAT CCCCCACTTC CAGAGATCCC CAACGGTGAA 
TGGCTCTGTC CCCGTTGTAC GTGTCCAGCT CTGAAGGGCA AAGTGCAGAA GATCCTAATC 
TGGAAGTGGG GTCAGCCACC ATCTCCCACA CCAGTGCCTC GGCCTCCAGA TGCTGATCCC 
AACACGCCCT CCCCAAAGCC CTTGGAGGGG CGGCCAGAGC GGCAGTTCTT TGTGAAATGG 
CAAGGCATGT CTTACTGGCA CTGCTCCTGG GTTTCTGAAC TGCAGCTGGA GCTGCACTGT 
CAGGTGATGT TCCGAAACTA TCAGCGGAAG AATGATATGG ATGAGCCACC TTCTGGGGAC 
TTTGGTGGTG ATGAAGAGAA AAGCCGAAAG CGAAAGAACA AGGACCCTAA ATTTGCAGAG 
ATGGAGGAAC GCTTCTATCG CTATGGGATA AAACCCGAGT GGATGATGAT CCACCGAATC 
CTCAACCACA GTGTGGACAA GAAGGGCCAC GTCCACTACT TGATCAAGTG GCGGGACTTA 
CCTTACGATC AGGCTTCTTG GGAGAGTGAG GATGTGGAGA TCCAGGATTA CGACCTGTTC 
AAGCAGAGCT ATTGGAATCA CAGGGAGTTA ATGAGGGGTG AGGAAGGCCG ACCAGGCAAG 
AAGCTCAAGA AGGTGAAGCT TCGGAAGTTG GAGAGGCCTC CAGAAACGCC AACAGTTGAT 
CCAACAGTGA AGTATGAGCG ACAGCCAGAG TACCTGGATG CTACAGGTGG AACCCTGCAC 
CCCTATCAAA TGGAGGGCCT GAATTGGTTG CGCTTCTCCT GGGCTCAGGG CACTGACACC 
ATCTTGGCTG ATGAGATGGG CCTTGGGAAA ACTGTACAGA CAGCAGTCTT CCTGTATTCC 
CTTTACAAGG AGGGTCATTC CAAAGGCCCC TTCCTAGTGA GCGCCCCTCT TTCTACCATC 
ATCAACTGGG AGCGGGAGTT TGAAATGTGG GCTCCAGACA TGTATGTCGT AACCTATGTG 
GGTGACAAGG ACAGCCGTGC CATCATCCGA GAGAATGAGT TCTCCTTTGA AGACAATGCC 
ATTCGTGGTG GCAAGAAGGC CTCCCGCATG AAGAAAGAGG CATCTGTGAA ATTCCATGTG 
CTGCTGACAT CCTATGAATT GATCACCATT GACATGGCTA TTTTGGGCTC TATTGATTGG 
GCCTGCCTCA TCGTGGATGA AGCCCATCGG CTGAAGAACA ATCAGTCTAA GTTCTTCCGG 
GTATTGAATG GTTACTCACT CCAGCACAAG CTGTTGCTGA CTGGGACACC ATTACAAAAC 
AATCTGGAAG AGTTGTTTCA TCTGCTCAAC TTTCTCACCC CCGAGAGGTT CCACAATTTG 
GAAGGTTTTT TGGAGGAGTT TGCTGACATT GCCAAGGAGG ACCAGATAAA AAAACTGCAT 
GACATGCTGG GGCCGCACAT GTTGCGGCGG CTCAAAGCCG ATGTGTTCAA GAACATGCCC 
TCCAAGACAG AACTAATTGT GCGTGTGGAG CTGAGCCCTA TGCAGAAGAA ATACTACAAG 
TACATCCTCA CTCGAAATTT TGAAGCACTC AATGCCCGAG GTGGTGGCAA CCAGGTGTCT 
CTGCTGAATG TGGTGATGGA TCTTAAGAAG TGCTGCAACC ATCCATACCT CTTCCCTGTG 
GCTGCAATGG AAGCTCCTAA GATGCCTAAT GGCATGTATG ATGGCAGTGC CCTAATCAGA 
GCATCTGGGA AATTATTGCT GCTGCAGAAA ATGCTCAAGA ACCTTAAGGA GGGTGGGCAT 
CGTGTACTCA TCTTTTCCCA GATGACCAAG ATGCTAGACC TGCTAGAGGA TTTCTTGGAA 
CATGAAGGTT ATAAATACGA ACGCATCGAT GGTGGAATCA CTGGGAACAT GCGGCAAGAG 
GCCATTGACC GCTTCAATGC ACCGGGTGCT CAGCAGTTCT GCTTCTTGCT TTCCACTCGA 
GCTGGGGGCC TTGGAATCAA TCTGGCCACT GCTGACACAG TTATTATCTA TGACTCTGAC 
TGGAACCCCC ATAATGACAT TCAGGCCTTT AGCAGAGCTC ACCGGATTGG GCAAAATAAA 
AAGGTAATGA TCTACCGGTT TGTGACCCGT GCGTCAGTGG AGGAGCGCAT CACGCAGGTG 
GCAAAGAAGA AAATGATGCT GACGCATCTA GTGGTGCGGC CTGGGCTGGG CTCCAAGACT 
GGATCTATGT CCAAACAGGA GCTTGATGAT ATCCTCAAAT TTGGCACTGA GGAACTATTC 
AAGGATGAAG CCACTGATGG AGGAGGAGAC AACAAAGAGG GAGAAGATAG CAGTGTTATC 
CACTACGATG ATAAGGCCAT TGAACGGCTG CTAGACCGTA ACCAGGATGA GACTGAAGAC 
ACAGAATTGC AGGGCATGAA TGAATATTTG AGCTCATTCA AAGTGGCCCA GTATGTGGTA 
CGGGAAGAAG AAATGGGGGA GGAAGAGGAG GTAGAACGGG AAATCATTAA ACAGGAAGAA 
AGTGTGGATC CTGACTACTG GGAGAAATTG CTGCGGCACC ATTATGAGCA GCAGCAAGAA 
GATCTAGCCC GAAATCTGGG CAAAGGAAAA AGAATCCGTA AACAGGTCAA CTACAATGAT 
GGCTCCCAGG AGGACCGAGA TTGGCAGGAC GACCAGTCCG ACAACCAGTC CGATTACTCA 
GTGGCTTCAG AGGAAGGTGA TGAAGACTTT GATGAACGTT CAGAAGCTCC CCGTAGGCCC 
AGTCGTAAGG GCCTGCGGAA TGATAAAGAT AAGCCATTGC CTCCTCTGTT GGCCCGTGTT 
GGTGGGAATA TTGAAGTACT TGGTTTTAAT GCTCGTCAGC GAAAAGCCTT TCTTAATGCA 
ATTATGCGAT ATGGTATGCC ACCTCAGGAT GCTTTTACTA CCCAGTGGCT TGTAAGAGAC 
CTGCGAGGCA AATCAGAGAA AGAGTTCAAG GCATATGTCT CTCTTTTCAT GCGGCATTTA 
TGTGAGCCGG GGGCAGATGG GGCTGAGACC TTTGCTGATG GTGTCCCCCG AGAAGGCCTG 
TCTCGCCAGC ATGTCCTTAC TAGAATTGGT GTTATGTCTT TGATTCGCAA GAAGGTTCAG 
GAGTTTGAAC ATGTTAATGG GCGCTGGAGC ATGCCTGAAC TGGCTGAGGT GGAGGAAAAC 
AAGAAGATGT CCCAGCCAGG GTCACCCTCC CCAAAAACTC CTACACCCTC CACTCCAGGG 
GACACGCAGC CCAACACTCC TGCACCTGTC CCACCTGCTG AAGATGGGAT AAAAATAGAG 
GAAAATAGCC TCAAAGAAGA AGAGAGCATA GAAGGAGAAA AGGAGGTTAA ATCTACAGCC 
CCTGAGACTG CCATTGAGTG TACACAGGCC CCTGCCCCTG CCTCAGAGGA TGAAAAGGTC 
GTTGTTGAAC CCCCTGAGGG AGAGGAGAAA GTGGAAAAGG CAGAGGTGAA GGAGAGAACA 
GAGGAACCTA TGGAGACAGA GCCCAAAGGT AAAGGTGCTG CTGATGTAGA GAAGGTGGAG 
GAAAAGTCAG CAATAGATCT GACCCCTATT GTGGTAGAAG ACAAAGAAGA GAAGAAAGAA 
GAAGAAGAGA AAAAAGAGGT GATGCTTCAG AATGGAGAGA CCCCCAAGGA CCTGAATGAT 
GAGAAACAGA AGAAAAATAT TAAACAACGT TTCATGTTTA ACATTGCAGA TGGTGGTTTT 
ACTGAGTTGC ACTCCCTTTG GCAGAATGAA GAGCGGGCAG CCACAGTTAC CAAGAAGACT 
TATGAGATCT GGCATCGACG GCATGACTAC TGGCTGCTAG CCGGCATTAT AAACCATGGC 
TATGCCCGGT GGCAAGACAT CCAGAATGAC CCACGCTATG CCATCCTCAA TGAGCCTTTC 
AAGGGTGAAA TGAACCGTGG CAATTTCTTA GAGATCAAGA ATAAATTTCT AGCTCGAAGG 
TTTAAGCTCT TAGAACAAGC TCTGGTGATT GAGGAACAGC TGCGCCGGGC TGCTTACTTG 
AACATGTCAG AAGACCCTTC TCACCCTTCC ATGGCCCTCA ACACCCGCTT TGCTGAGGTG 
GAGTGTTTGG CGGAAAGTCA TCAGCACCTG TCCAAGGAGT CAATGGCAGG AAACAAGCCA 
GCCAATGCAG TCCTGCACAA AGGTATTCTG AAACAGCTGG AAGAACTGCT GAGTGACATG 
AAAGCTGATG TGACTCGACT CCCAGCTACC ATTGCCCGAA TTCCCCCAGT TGCTGTGAGG 
TTACAGATGT CAGAGCGTAA CATTCTCAGC CGCCTGGCAA ACCGGGCACC CGAACCTACC 
CCACAGCAGG TAGCCCAGCA GCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHD4 ( XM_006718962.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_006719025.1 Homo sapiens chromodomain helicase DNA binding protein 4 (CHD4), transcript variant X11, mRNA.

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CloneID	OHu45954
Clone ID Related Accession (Same CDS sequence)	XM_006718962.1 , NM_001363606.1 , NM_001363606.2
Accession Version	XM_006718962.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5709bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	chromodomain-helicase-DNA-binding protein 4 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCGG GCCTGGGCTC CCCGTCCCCC TGCTCGGCGG GCAGTGAGGA GGAGGATATG 
GATGCACTTT TGAACAACAG CCTGCCCCCA CCCCACCCAG AAAATGAAGA GGACCCAGAA 
GAGGATTTGT CAGAAACAGA GACTCCAAAG CTCAAGAAGA AGAAAAAGCC TAAGAAACCT 
CGGGACCCTA AAATCCCTAA GAGCAAGCGC CAAAAAAAGG AGCGTATGCT CTTATGCCGG 
CAGCTGGGGG ACAGCTCTGG GGAGGGGCCA GAGTTTGTGG AGGAGGAGGA AGAGGTGGCT 
CTGCGCTCAG ACAGTGAGGG CAGCGACTAT ACTCCTGGCA AGAAGAAGAA GAAGAAGCTT 
GGACCTAAGA AAGAGAAGAA GAGCAAATCC AAGCGGAAGG AGGAGGAGGA GGAGGAGGAT 
GATGATGATG ATTCAAAGGA GCCTAAATCA TCTGCTCAGC TCCTGGAAGA CTGGGGCATG 
GAAGACATTG ACCACGTGTT CTCAGAGGAG GATTATCGAA CCCTCACCAA CTACAAGGCC 
TTCAGCCAGT TTGTCAGACC CCTCATTGCT GCCAAAAATC CCAAGATTGC TGTCTCCAAG 
ATGATGATGG TTTTGGGTGC AAAATGGCGG GAGTTCAGTA CCAATAACCC CTTCAAAGGC 
AGTTCTGGGG CATCAGTGGC AGCTGCGGCA GCAGCAGCGG TAGCTGTGGT GGAGAGCATG 
GTGACAGCCA CTGAGGTTGC ACCACCACCT CCCCCTGTGG AGGTGCCTAT CCGCAAGGCC 
AAGACCAAGG AGGGCAAAGG TCCCAATGCT CGGAGGAAGC CCAAGGGCAG CCCTCGTGTA 
CCTGATGCCA AGAAGCCTAA ACCCAAGAAA GTAGCTCCCC TGAAAATCAA GCTGGGAGGT 
TTTGGTTCCA AGCGTAAGAG ATCCTCGAGT GAGGATGATG ACTTAGATGT GGAATCTGAC 
TTCGATGATG CCAGTATCAA TAGCTATTCT GTTTCTGATG GTTCCACCAG CCGTAGTAGC 
CGCAGCCGCA AGAAACTCCG AACCACTAAA AAGAAAAAGA AAGACCACCA GGACTATTGC 
GAGGTGTGCC AGCAAGGCGG TGAGATCATC CTGTGTGATA CCTGTCCCCG TGCTTACCAC 
ATGGTCTGCC TGGATCCCGA CATGGAGAAG GCTCCCGAGG GCAAGTGGAG CTGCCCACAC 
TGCGAGAAGG AAGGCATCCA GTGGGAAGCT AAAGAGGACA ATTCGGAGGG TGAGGAGATC 
CTGGAAGAGG TTGGGGGAGA CCTCGAAGAG GAGGATGACC ACCATATGGA ATTCTGTCGG 
GTCTGCAAGG ATGGTGGGGA ACTGCTCTGC TGTGATACCT GTCCTTCTTC CTACCACATC 
CACTGCCTGA ATCCCCCACT TCCAGAGATC CCCAACGGTG AATGGCTCTG TCCCCGTTGT 
ACGTGTCCAG CTCTGAAGGG CAAAGTGCAG AAGATCCTAA TCTGGAAGTG GGGTCAGCCA 
CCATCTCCCA CACCAGTGCC TCGGCCTCCA GATGCTGATC CCAACACGCC CTCCCCAAAG 
CCCTTGGAGG GGCGGCCAGA GCGGCAGTTC TTTGTGAAAT GGCAAGGCAT GTCTTACTGG 
CACTGCTCCT GGGTTTCTGA ACTGCAGCTG GAGCTGCACT GTCAGGTGAT GTTCCGAAAC 
TATCAGCGGA AGAATGATAT GGATGAGCCA CCTTCTGGGG ACTTTGGTGG TGATGAAGAG 
AAAAGCCGAA AGCGAAAGAA CAAGGACCCT AAATTTGCAG AGATGGAGGA ACGCTTCTAT 
CGCTATGGGA TAAAACCCGA GTGGATGATG ATCCACCGAA TCCTCAACCA CAGTGTGGAC 
AAGAAGGGCC ACGTCCACTA CTTGATCAAG TGGCGGGACT TACCTTACGA TCAGGCTTCT 
TGGGAGAGTG AGGATGTGGA GATCCAGGAT TACGACCTGT TCAAGCAGAG CTATTGGAAT 
CACAGGGAGT TAATGAGGGG TGAGGAAGGC CGACCAGGCA AGAAGCTCAA GAAGGTGAAG 
CTTCGGAAGT TGGAGAGGCC TCCAGAAACG CCAACAGTTG ATCCAACAGT GAAGTATGAG 
CGACAGCCAG AGTACCTGGA TGCTACAGGT GGAACCCTGC ACCCCTATCA AATGGAGGGC 
CTGAATTGGT TGCGCTTCTC CTGGGCTCAG GGCACTGACA CCATCTTGGC TGATGAGATG 
GGCCTTGGGA AAACTGTACA GACAGCAGTC TTCCTGTATT CCCTTTACAA GGAGGGTCAT 
TCCAAAGGCC CCTTCCTAGT GAGCGCCCCT CTTTCTACCA TCATCAACTG GGAGCGGGAG 
TTTGAAATGT GGGCTCCAGA CATGTATGTC GTAACCTATG TGGGTGACAA GGACAGCCGT 
GCCATCATCC GAGAGAATGA GTTCTCCTTT GAAGACAATG CCATTCGTGG TGGCAAGAAG 
GCCTCCCGCA TGAAGAAAGA GGCATCTGTG AAATTCCATG TGCTGCTGAC ATCCTATGAA 
TTGATCACCA TTGACATGGC TATTTTGGGC TCTATTGATT GGGCCTGCCT CATCGTGGAT 
GAAGCCCATC GGCTGAAGAA CAATCAGTCT AAGTTCTTCC GGGTATTGAA TGGTTACTCA 
CTCCAGCACA AGCTGTTGCT GACTGGGACA CCATTACAAA ACAATCTGGA AGAGTTGTTT 
CATCTGCTCA ACTTTCTCAC CCCCGAGAGG TTCCACAATT TGGAAGGTTT TTTGGAGGAG 
TTTGCTGACA TTGCCAAGGA GGACCAGATA AAAAAACTGC ATGACATGCT GGGGCCGCAC 
ATGTTGCGGC GGCTCAAAGC CGATGTGTTC AAGAACATGC CCTCCAAGAC AGAACTAATT 
GTGCGTGTGG AGCTGAGCCC TATGCAGAAG AAATACTACA AGTACATCCT CACTCGAAAT 
TTTGAAGCAC TCAATGCCCG AGGTGGTGGC AACCAGGTGT CTCTGCTGAA TGTGGTGATG 
GATCTTAAGA AGTGCTGCAA CCATCCATAC CTCTTCCCTG TGGCTGCAAT GGAAGCTCCT 
AAGATGCCTA ATGGCATGTA TGATGGCAGT GCCCTAATCA GAGCATCTGG GAAATTATTG 
CTGCTGCAGA AAATGCTCAA GAACCTTAAG GAGGGTGGGC ATCGTGTACT CATCTTTTCC 
CAGATGACCA AGATGCTAGA CCTGCTAGAG GATTTCTTGG AACATGAAGG TTATAAATAC 
GAACGCATCG ATGGTGGAAT CACTGGGAAC ATGCGGCAAG AGGCCATTGA CCGCTTCAAT 
GCACCGGGTG CTCAGCAGTT CTGCTTCTTG CTTTCCACTC GAGCTGGGGG CCTTGGAATC 
AATCTGGCCA CTGCTGACAC AGTTATTATC TATGACTCTG ACTGGAACCC CCATAATGAC 
ATTCAGGCCT TTAGCAGAGC TCACCGGATT GGGCAAAATA AAAAGGTAAT GATCTACCGG 
TTTGTGACCC GTGCGTCAGT GGAGGAGCGC ATCACGCAGG TGGCAAAGAA GAAAATGATG 
CTGACGCATC TAGTGGTGCG GCCTGGGCTG GGCTCCAAGA CTGGATCTAT GTCCAAACAG 
GAGCTTGATG ATATCCTCAA ATTTGGCACT GAGGAACTAT TCAAGGATGA AGCCACTGAT 
GGAGGAGGAG ACAACAAAGA GGGAGAAGAT AGCAGTGTTA TCCACTACGA TGATAAGGCC 
ATTGAACGGC TGCTAGACCG TAACCAGGAT GAGACTGAAG ACACAGAATT GCAGGGCATG 
AATGAATATT TGAGCTCATT CAAAGTGGCC CAGTATGTGG TACGGGAAGA AGAAATGGGG 
GAGGAAGAGG AGGTAGAACG GGAAATCATT AAACAGGAAG AAAGTGTGGA TCCTGACTAC 
TGGGAGAAAT TGCTGCGGCA CCATTATGAG CAGCAGCAAG AAGATCTAGC CCGAAATCTG 
GGCAAAGGAA AAAGAATCCG TAAACAGGTC AACTACAATG ATGGCTCCCA GGAGGACCGA 
GATTGGCAGG ACGACCAGTC CGACAACCAG TCCGATTACT CAGTGGCTTC AGAGGAAGGT 
GATGAAGACT TTGATGAACG TTCAGAAGCT CCCCGTAGGC CCAGTCGTAA GGGCCTGCGG 
AATGATAAAG ATAAGCCATT GCCTCCTCTG TTGGCCCGTG TTGGTGGGAA TATTGAAGTA 
CTTGGTTTTA ATGCTCGTCA GCGAAAAGCC TTTCTTAATG CAATTATGCG ATATGGTATG 
CCACCTCAGG ATGCTTTTAC TACCCAGTGG CTTGTAAGAG ACCTGCGAGG CAAATCAGAG 
AAAGAGTTCA AGGCATATGT CTCTCTTTTC ATGCGGCATT TATGTGAGCC GGGGGCAGAT 
GGGGCTGAGA CCTTTGCTGA TGGTGTCCCC CGAGAAGGCC TGTCTCGCCA GCATGTCCTT 
ACTAGAATTG GTGTTATGTC TTTGATTCGC AAGAAGGTTC AGGAGTTTGA ACATGTTAAT 
GGGCGCTGGA GCATGCCTGA ACTGGCTGAG GTGGAGGAAA ACAAGAAGAT GTCCCAGCCA 
GGGTCACCCT CCCCAAAAAC TCCTACACCC TCCACTCCAG GGGACACGCA GCCCAACACT 
CCTGCACCTG TCCCACCTGC TGAAGATGGG ATAAAAATAG AGGAAAATAG CCTCAAAGAA 
GAAGAGAGCA TAGAAGGAGA AAAGGAGGTT AAATCTACAG CCCCTGAGAC TGCCATTGAG 
TGTACACAGG CCCCTGCCCC TGCCTCAGAG GATGAAAAGG TCGTTGTTGA ACCCCCTGAG 
GGAGAGGAGA AAGTGGAAAA GGCAGAGGTG AAGGAGAGAA CAGAGGAACC TATGGAGACA 
GAGCCCAAAG GTAAAGGTGC TGCTGATGTA GAGAAGGTGG AGGAAAAGTC AGCAATAGAT 
CTGACCCCTA TTGTGGTAGA AGACAAAGAA GAGAAGAAAG AAGAAGAAGA GAAAAAAGAG 
GTGATGCTTC AGAATGGAGA GACCCCCAAG GACCTGAATG ATGAGAAACA GAAGAAAAAT 
ATTAAACAAC GTTTCATGTT TAACATTGCA GATGGTGGTT TTACTGAGTT GCACTCCCTT 
TGGCAGAATG AAGAGCGGGC AGCCACAGTT ACCAAGAAGA CTTATGAGAT CTGGCATCGA 
CGGCATGACT ACTGGCTGCT AGCCGGCATT ATAAACCATG GCTATGCCCG GTGGCAAGAC 
ATCCAGAATG ACCCACGCTA TGCCATCCTC AATGAGCCTT TCAAGGGTGA AATGAACCGT 
GGCAATTTCT TAGAGATCAA GAATAAATTT CTAGCTCGAA GGTTTAAGCT CTTAGAACAA 
GCTCTGGTGA TTGAGGAACA GCTGCGCCGG GCTGCTTACT TGAACATGTC AGAAGACCCT 
TCTCACCCTT CCATGGCCCT CAACACCCGC TTTGCTGAGG TGGAGTGTTT GGCGGAAAGT 
CATCAGCACC TGTCCAAGGA GTCAATGGCA GGAAACAAGC CAGCCAATGC AGTCCTGCAC 
AAAGGTATTC TGAAACAGCT GGAAGAACTG CTGAGTGACA TGAAAGCTGA TGTGACTCGA 
CTCCCAGCTA CCATTGCCCG AATTCCCCCA GTTGCTGTGA GGTTACAGAT GTCAGAGCGT 
AACATTCTCA GCCGCCTGGC AAACCGGGCA CCCGAACCTA CCCCACAGCA GGTAGCCCAG 
CAGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006719025.1
CDS	219..5927
Translation	MASGLGSPSPCSAGSEEEDMDALLNNSLPPPHPENEEDPEEDLSETETPKLKKKKKPKKPRDPKIPKSKRQKKERMLLCRQLGDSSGEGPEFVEEEEEVALRSDSEGSDYTPGKKKKKKLGPKKEKKSKSKRKEEEEEEDDDDDSKEPKSSAQLLEDWGMEDIDHVFSEEDYRTLTNYKAFSQFVRPLIAAKNPKIAVSKMMMVLGAKWREFSTNNPFKGSSGASVAAAAAAAVAVVESMVTATEVAPPPPPVEVPIRKAKTKEGKGPNARRKPKGSPRVPDAKKPKPKKVAPLKIKLGGFGSKRKRSSSEDDDLDVESDFDDASINSYSVSDGSTSRSSRSRKKLRTTKKKKKDHQDYCEVCQQGGEIILCDTCPRAYHMVCLDPDMEKAPEGKWSCPHCEKEGIQWEAKEDNSEGEEILEEVGGDLEEEDDHHMEFCRVCKDGGELLCCDTCPSSYHIHCLNPPLPEIPNGEWLCPRCTCPALKGKVQKILIWKWGQPPSPTPVPRPPDADPNTPSPKPLEGRPERQFFVKWQGMSYWHCSWVSELQLELHCQVMFRNYQRKNDMDEPPSGDFGGDEEKSRKRKNKDPKFAEMEERFYRYGIKPEWMMIHRILNHSVDKKGHVHYLIKWRDLPYDQASWESEDVEIQDYDLFKQSYWNHRELMRGEEGRPGKKLKKVKLRKLERPPETPTVDPTVKYERQPEYLDATGGTLHPYQMEGLNWLRFSWAQGTDTILADEMGLGKTVQTAVFLYSLYKEGHSKGPFLVSAPLSTIINWEREFEMWAPDMYVVTYVGDKDSRAIIRENEFSFEDNAIRGGKKASRMKKEASVKFHVLLTSYELITIDMAILGSIDWACLIVDEAHRLKNNQSKFFRVLNGYSLQHKLLLTGTPLQNNLEELFHLLNFLTPERFHNLEGFLEEFADIAKEDQIKKLHDMLGPHMLRRLKADVFKNMPSKTELIVRVELSPMQKKYYKYILTRNFEALNARGGGNQVSLLNVVMDLKKCCNHPYLFPVAAMEAPKMPNGMYDGSALIRASGKLLLLQKMLKNLKEGGHRVLIFSQMTKMLDLLEDFLEHEGYKYERIDGGITGNMRQEAIDRFNAPGAQQFCFLLSTRAGGLGINLATADTVIIYDSDWNPHNDIQAFSRAHRIGQNKKVMIYRFVTRASVEERITQVAKKKMMLTHLVVRPGLGSKTGSMSKQELDDILKFGTEELFKDEATDGGGDNKEGEDSSVIHYDDKAIERLLDRNQDETEDTELQGMNEYLSSFKVAQYVVREEEMGEEEEVEREIIKQEESVDPDYWEKLLRHHYEQQQEDLARNLGKGKRIRKQVNYNDGSQEDRDWQDDQSDNQSDYSVASEEGDEDFDERSEAPRRPSRKGLRNDKDKPLPPLLARVGGNIEVLGFNARQRKAFLNAIMRYGMPPQDAFTTQWLVRDLRGKSEKEFKAYVSLFMRHLCEPGADGAETFADGVPREGLSRQHVLTRIGVMSLIRKKVQEFEHVNGRWSMPELAEVEENKKMSQPGSPSPKTPTPSTPGDTQPNTPAPVPPAEDGIKIEENSLKEEESIEGEKEVKSTAPETAIECTQAPAPASEDEKVVVEPPEGEEKVEKAEVKERTEEPMETEPKGKGAADVEKVEEKSAIDLTPIVVEDKEEKKEEEEKKEVMLQNGETPKDLNDEKQKKNIKQRFMFNIADGGFTELHSLWQNEERAATVTKKTYEIWHRRHDYWLLAGIINHGYARWQDIQNDPRYAILNEPFKGEMNRGNFLEIKNKFLARRFKLLEQALVIEEQLRRAAYLNMSEDPSHPSMALNTRFAEVECLAESHQHLSKESMAGNKPANAVLHKGILKQLEELLSDMKADVTRLPATIARIPPVAVRLQMSERNILSRLANRAPEPTPQQVAQQQ

Target ORF information:

RefSeq Version	XM_006718962.1
Organism	Homo sapiens(human)
Definition	Homo sapiens chromodomain helicase DNA binding protein 4 (CHD4), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006718962.1

ORF Insert Sequence:

ATGGCGTCGG GCCTGGGCTC CCCGTCCCCC TGCTCGGCGG GCAGTGAGGA GGAGGATATG 
GATGCACTTT TGAACAACAG CCTGCCCCCA CCCCACCCAG AAAATGAAGA GGACCCAGAA 
GAGGATTTGT CAGAAACAGA GACTCCAAAG CTCAAGAAGA AGAAAAAGCC TAAGAAACCT 
CGGGACCCTA AAATCCCTAA GAGCAAGCGC CAAAAAAAGG AGCGTATGCT CTTATGCCGG 
CAGCTGGGGG ACAGCTCTGG GGAGGGGCCA GAGTTTGTGG AGGAGGAGGA AGAGGTGGCT 
CTGCGCTCAG ACAGTGAGGG CAGCGACTAT ACTCCTGGCA AGAAGAAGAA GAAGAAGCTT 
GGACCTAAGA AAGAGAAGAA GAGCAAATCC AAGCGGAAGG AGGAGGAGGA GGAGGAGGAT 
GATGATGATG ATTCAAAGGA GCCTAAATCA TCTGCTCAGC TCCTGGAAGA CTGGGGCATG 
GAAGACATTG ACCACGTGTT CTCAGAGGAG GATTATCGAA CCCTCACCAA CTACAAGGCC 
TTCAGCCAGT TTGTCAGACC CCTCATTGCT GCCAAAAATC CCAAGATTGC TGTCTCCAAG 
ATGATGATGG TTTTGGGTGC AAAATGGCGG GAGTTCAGTA CCAATAACCC CTTCAAAGGC 
AGTTCTGGGG CATCAGTGGC AGCTGCGGCA GCAGCAGCGG TAGCTGTGGT GGAGAGCATG 
GTGACAGCCA CTGAGGTTGC ACCACCACCT CCCCCTGTGG AGGTGCCTAT CCGCAAGGCC 
AAGACCAAGG AGGGCAAAGG TCCCAATGCT CGGAGGAAGC CCAAGGGCAG CCCTCGTGTA 
CCTGATGCCA AGAAGCCTAA ACCCAAGAAA GTAGCTCCCC TGAAAATCAA GCTGGGAGGT 
TTTGGTTCCA AGCGTAAGAG ATCCTCGAGT GAGGATGATG ACTTAGATGT GGAATCTGAC 
TTCGATGATG CCAGTATCAA TAGCTATTCT GTTTCTGATG GTTCCACCAG CCGTAGTAGC 
CGCAGCCGCA AGAAACTCCG AACCACTAAA AAGAAAAAGA AAGACCACCA GGACTATTGC 
GAGGTGTGCC AGCAAGGCGG TGAGATCATC CTGTGTGATA CCTGTCCCCG TGCTTACCAC 
ATGGTCTGCC TGGATCCCGA CATGGAGAAG GCTCCCGAGG GCAAGTGGAG CTGCCCACAC 
TGCGAGAAGG AAGGCATCCA GTGGGAAGCT AAAGAGGACA ATTCGGAGGG TGAGGAGATC 
CTGGAAGAGG TTGGGGGAGA CCTCGAAGAG GAGGATGACC ACCATATGGA ATTCTGTCGG 
GTCTGCAAGG ATGGTGGGGA ACTGCTCTGC TGTGATACCT GTCCTTCTTC CTACCACATC 
CACTGCCTGA ATCCCCCACT TCCAGAGATC CCCAACGGTG AATGGCTCTG TCCCCGTTGT 
ACGTGTCCAG CTCTGAAGGG CAAAGTGCAG AAGATCCTAA TCTGGAAGTG GGGTCAGCCA 
CCATCTCCCA CACCAGTGCC TCGGCCTCCA GATGCTGATC CCAACACGCC CTCCCCAAAG 
CCCTTGGAGG GGCGGCCAGA GCGGCAGTTC TTTGTGAAAT GGCAAGGCAT GTCTTACTGG 
CACTGCTCCT GGGTTTCTGA ACTGCAGCTG GAGCTGCACT GTCAGGTGAT GTTCCGAAAC 
TATCAGCGGA AGAATGATAT GGATGAGCCA CCTTCTGGGG ACTTTGGTGG TGATGAAGAG 
AAAAGCCGAA AGCGAAAGAA CAAGGACCCT AAATTTGCAG AGATGGAGGA ACGCTTCTAT 
CGCTATGGGA TAAAACCCGA GTGGATGATG ATCCACCGAA TCCTCAACCA CAGTGTGGAC 
AAGAAGGGCC ACGTCCACTA CTTGATCAAG TGGCGGGACT TACCTTACGA TCAGGCTTCT 
TGGGAGAGTG AGGATGTGGA GATCCAGGAT TACGACCTGT TCAAGCAGAG CTATTGGAAT 
CACAGGGAGT TAATGAGGGG TGAGGAAGGC CGACCAGGCA AGAAGCTCAA GAAGGTGAAG 
CTTCGGAAGT TGGAGAGGCC TCCAGAAACG CCAACAGTTG ATCCAACAGT GAAGTATGAG 
CGACAGCCAG AGTACCTGGA TGCTACAGGT GGAACCCTGC ACCCCTATCA AATGGAGGGC 
CTGAATTGGT TGCGCTTCTC CTGGGCTCAG GGCACTGACA CCATCTTGGC TGATGAGATG 
GGCCTTGGGA AAACTGTACA GACAGCAGTC TTCCTGTATT CCCTTTACAA GGAGGGTCAT 
TCCAAAGGCC CCTTCCTAGT GAGCGCCCCT CTTTCTACCA TCATCAACTG GGAGCGGGAG 
TTTGAAATGT GGGCTCCAGA CATGTATGTC GTAACCTATG TGGGTGACAA GGACAGCCGT 
GCCATCATCC GAGAGAATGA GTTCTCCTTT GAAGACAATG CCATTCGTGG TGGCAAGAAG 
GCCTCCCGCA TGAAGAAAGA GGCATCTGTG AAATTCCATG TGCTGCTGAC ATCCTATGAA 
TTGATCACCA TTGACATGGC TATTTTGGGC TCTATTGATT GGGCCTGCCT CATCGTGGAT 
GAAGCCCATC GGCTGAAGAA CAATCAGTCT AAGTTCTTCC GGGTATTGAA TGGTTACTCA 
CTCCAGCACA AGCTGTTGCT GACTGGGACA CCATTACAAA ACAATCTGGA AGAGTTGTTT 
CATCTGCTCA ACTTTCTCAC CCCCGAGAGG TTCCACAATT TGGAAGGTTT TTTGGAGGAG 
TTTGCTGACA TTGCCAAGGA GGACCAGATA AAAAAACTGC ATGACATGCT GGGGCCGCAC 
ATGTTGCGGC GGCTCAAAGC CGATGTGTTC AAGAACATGC CCTCCAAGAC AGAACTAATT 
GTGCGTGTGG AGCTGAGCCC TATGCAGAAG AAATACTACA AGTACATCCT CACTCGAAAT 
TTTGAAGCAC TCAATGCCCG AGGTGGTGGC AACCAGGTGT CTCTGCTGAA TGTGGTGATG 
GATCTTAAGA AGTGCTGCAA CCATCCATAC CTCTTCCCTG TGGCTGCAAT GGAAGCTCCT 
AAGATGCCTA ATGGCATGTA TGATGGCAGT GCCCTAATCA GAGCATCTGG GAAATTATTG 
CTGCTGCAGA AAATGCTCAA GAACCTTAAG GAGGGTGGGC ATCGTGTACT CATCTTTTCC 
CAGATGACCA AGATGCTAGA CCTGCTAGAG GATTTCTTGG AACATGAAGG TTATAAATAC 
GAACGCATCG ATGGTGGAAT CACTGGGAAC ATGCGGCAAG AGGCCATTGA CCGCTTCAAT 
GCACCGGGTG CTCAGCAGTT CTGCTTCTTG CTTTCCACTC GAGCTGGGGG CCTTGGAATC 
AATCTGGCCA CTGCTGACAC AGTTATTATC TATGACTCTG ACTGGAACCC CCATAATGAC 
ATTCAGGCCT TTAGCAGAGC TCACCGGATT GGGCAAAATA AAAAGGTAAT GATCTACCGG 
TTTGTGACCC GTGCGTCAGT GGAGGAGCGC ATCACGCAGG TGGCAAAGAA GAAAATGATG 
CTGACGCATC TAGTGGTGCG GCCTGGGCTG GGCTCCAAGA CTGGATCTAT GTCCAAACAG 
GAGCTTGATG ATATCCTCAA ATTTGGCACT GAGGAACTAT TCAAGGATGA AGCCACTGAT 
GGAGGAGGAG ACAACAAAGA GGGAGAAGAT AGCAGTGTTA TCCACTACGA TGATAAGGCC 
ATTGAACGGC TGCTAGACCG TAACCAGGAT GAGACTGAAG ACACAGAATT GCAGGGCATG 
AATGAATATT TGAGCTCATT CAAAGTGGCC CAGTATGTGG TACGGGAAGA AGAAATGGGG 
GAGGAAGAGG AGGTAGAACG GGAAATCATT AAACAGGAAG AAAGTGTGGA TCCTGACTAC 
TGGGAGAAAT TGCTGCGGCA CCATTATGAG CAGCAGCAAG AAGATCTAGC CCGAAATCTG 
GGCAAAGGAA AAAGAATCCG TAAACAGGTC AACTACAATG ATGGCTCCCA GGAGGACCGA 
GATTGGCAGG ACGACCAGTC CGACAACCAG TCCGATTACT CAGTGGCTTC AGAGGAAGGT 
GATGAAGACT TTGATGAACG TTCAGAAGCT CCCCGTAGGC CCAGTCGTAA GGGCCTGCGG 
AATGATAAAG ATAAGCCATT GCCTCCTCTG TTGGCCCGTG TTGGTGGGAA TATTGAAGTA 
CTTGGTTTTA ATGCTCGTCA GCGAAAAGCC TTTCTTAATG CAATTATGCG ATATGGTATG 
CCACCTCAGG ATGCTTTTAC TACCCAGTGG CTTGTAAGAG ACCTGCGAGG CAAATCAGAG 
AAAGAGTTCA AGGCATATGT CTCTCTTTTC ATGCGGCATT TATGTGAGCC GGGGGCAGAT 
GGGGCTGAGA CCTTTGCTGA TGGTGTCCCC CGAGAAGGCC TGTCTCGCCA GCATGTCCTT 
ACTAGAATTG GTGTTATGTC TTTGATTCGC AAGAAGGTTC AGGAGTTTGA ACATGTTAAT 
GGGCGCTGGA GCATGCCTGA ACTGGCTGAG GTGGAGGAAA ACAAGAAGAT GTCCCAGCCA 
GGGTCACCCT CCCCAAAAAC TCCTACACCC TCCACTCCAG GGGACACGCA GCCCAACACT 
CCTGCACCTG TCCCACCTGC TGAAGATGGG ATAAAAATAG AGGAAAATAG CCTCAAAGAA 
GAAGAGAGCA TAGAAGGAGA AAAGGAGGTT AAATCTACAG CCCCTGAGAC TGCCATTGAG 
TGTACACAGG CCCCTGCCCC TGCCTCAGAG GATGAAAAGG TCGTTGTTGA ACCCCCTGAG 
GGAGAGGAGA AAGTGGAAAA GGCAGAGGTG AAGGAGAGAA CAGAGGAACC TATGGAGACA 
GAGCCCAAAG GTAAAGGTGC TGCTGATGTA GAGAAGGTGG AGGAAAAGTC AGCAATAGAT 
CTGACCCCTA TTGTGGTAGA AGACAAAGAA GAGAAGAAAG AAGAAGAAGA GAAAAAAGAG 
GTGATGCTTC AGAATGGAGA GACCCCCAAG GACCTGAATG ATGAGAAACA GAAGAAAAAT 
ATTAAACAAC GTTTCATGTT TAACATTGCA GATGGTGGTT TTACTGAGTT GCACTCCCTT 
TGGCAGAATG AAGAGCGGGC AGCCACAGTT ACCAAGAAGA CTTATGAGAT CTGGCATCGA 
CGGCATGACT ACTGGCTGCT AGCCGGCATT ATAAACCATG GCTATGCCCG GTGGCAAGAC 
ATCCAGAATG ACCCACGCTA TGCCATCCTC AATGAGCCTT TCAAGGGTGA AATGAACCGT 
GGCAATTTCT TAGAGATCAA GAATAAATTT CTAGCTCGAA GGTTTAAGCT CTTAGAACAA 
GCTCTGGTGA TTGAGGAACA GCTGCGCCGG GCTGCTTACT TGAACATGTC AGAAGACCCT 
TCTCACCCTT CCATGGCCCT CAACACCCGC TTTGCTGAGG TGGAGTGTTT GGCGGAAAGT 
CATCAGCACC TGTCCAAGGA GTCAATGGCA GGAAACAAGC CAGCCAATGC AGTCCTGCAC 
AAAGGTATTC TGAAACAGCT GGAAGAACTG CTGAGTGACA TGAAAGCTGA TGTGACTCGA 
CTCCCAGCTA CCATTGCCCG AATTCCCCCA GTTGCTGTGA GGTTACAGAT GTCAGAGCGT 
AACATTCTCA GCCGCCTGGC AAACCGGGCA CCCGAACCTA CCCCACAGCA GGTAGCCCAG 
CAGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHD4 ( XM_017018725.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016874214.1 Homo sapiens chromodomain helicase DNA binding protein 4 (CHD4), transcript variant X7, mRNA.

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CloneID	OHu83470
Clone ID Related Accession (Same CDS sequence)	XM_017018725.1
Accession Version	XM_017018725.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5745bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-25
Organism	Homo sapiens(human)
Product	chromodomain-helicase-DNA-binding protein 4 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCGG GCCTGGGCTC CCCGTCCCCC TGCTCGGCGG GCAGTGAGGA GGAGGATATG 
GATGCACTTT TGAACAACAG CCTGCCCCCA CCCCACCCAG AAAATGAAGA GGACCCAGAA 
GAGGATTTGT CAGAAACAGA GACTCCAAAG CTCAAGAAGA AGAAAAAGCC TAAGAAACCT 
CGGGACCCTA AAATCCCTAA GAGCAAGCGC CAAAAAAAGG AGCGTATGCT CTTATGCCGG 
CAGCTGGGGG ACAGCTCTGG GGAGGGGCCA GAGTTTGTGG AGGAGGAGGA AGAGGTGGCT 
CTGCGCTCAG ACAGTGAGGG CAGCGACTAT ACTCCTGGCA AGAAGAAGAA GAAGAAGCTT 
GGACCTAAGA AAGAGAAGAA GAGCAAATCC AAGCGGAAGG AGGAGGAGGA GGAGGAGGAT 
GATGATGATG ATTCAAAGGA GCCTAAATCA TCTGCTCAGC TCCTGGAAGA CTGGGGCATG 
GAAGACATTG ACCACGTGTT CTCAGAGGAG GATTATCGAA CCCTCACCAA CTACAAGGCC 
TTCAGCCAGT TTGTCAGACC CCTCATTGCT GCCAAAAATC CCAAGATTGC TGTCTCCAAG 
ATGATGATGG TTTTGGGTGC AAAATGGCGG GAGTTCAGTA CCAATAACCC CTTCAAAGGC 
AGTTCTGGGG CATCAGTGGC AGCTGCGGCA GCAGCAGCGG TAGCTGTGGT GGAGAGCATG 
GTGACAGCCA CTGAGGTTGC ACCACCACCT CCCCCTGTGG AGGTGCCTAT CCGCAAGGCC 
AAGACCAAGG AGGGCAAAGG TCCCAATGCT CGGAGGAAGC CCAAGGGCAG CCCTCGTGTA 
CCTGATGCCA AGAAGCCTAA ACCCAAGAAA GTAGCTCCCC TGAAAATCAA GCTGGGAGGT 
TTTGGTTCCA AGCGTAAGAG ATCCTCGAGT GAGGATGATG ACTTAGATGT GGAATCTGAC 
TTCGATGATG CCAGTATCAA TAGCTATTCT GTTTCTGATG GTTCCACCAG CCGTAGTAGC 
CGCAGCCGCA AGAAACTCCG AACCACTAAA AAGAAAAAGA AAGGCGAGGA GGAGGTGACT 
GCTGTGGATG GTTATGAGAC AGACCACCAG GACTATTGCG AGGTGTGCCA GCAAGGCGGT 
GAGATCATCC TGTGTGATAC CTGTCCCCGT GCTTACCACA TGGTCTGCCT GGATCCCGAC 
ATGGAGAAGG CTCCCGAGGG CAAGTGGAGC TGCCCACACT GCGAGAAGGA AGGCATCCAG 
TGGGAAGCTA AAGAGGACAA TTCGGAGGGT GAGGAGATCC TGGAAGAGGT TGGGGGAGAC 
CTCGAAGAGG AGGATGACCA CCATATGGAA TTCTGTCGGG TCTGCAAGGA TGGTGGGGAA 
CTGCTCTGCT GTGATACCTG TCCTTCTTCC TACCACATCC ACTGCCTGAA TCCCCCACTT 
CCAGAGATCC CCAACGGTGA ATGGCTCTGT CCCCGTTGTA CGTGTCCAGC TCTGAAGGGC 
AAAGTGCAGA AGATCCTAAT CTGGAAGTGG GGTCAGCCAC CATCTCCCAC ACCAGTGCCT 
CGGCCTCCAG ATGCTGATCC CAACACGCCC TCCCCAAAGC CCTTGGAGGG GCGGCCAGAG 
CGGCAGTTCT TTGTGAAATG GCAAGGCATG TCTTACTGGC ACTGCTCCTG GGTTTCTGAA 
CTGCAGCTGG AGCTGCACTG TCAGGTGATG TTCCGAAACT ATCAGCGGAA GAATGATATG 
GATGAGCCAC CTTCTGGGGA CTTTGGTGGT GATGAAGAGA AAAGCCGAAA GCGAAAGAAC 
AAGGACCCTA AATTTGCAGA GATGGAGGAA CGCTTCTATC GCTATGGGAT AAAACCCGAG 
TGGATGATGA TCCACCGAAT CCTCAACCAC AGTGTGGACA AGAAGGGCCA CGTCCACTAC 
TTGATCAAGT GGCGGGACTT ACCTTACGAT CAGGCTTCTT GGGAGAGTGA GGATGTGGAG 
ATCCAGGATT ACGACCTGTT CAAGCAGAGC TATTGGAATC ACAGGGAGTT AATGAGGGGT 
GAGGAAGGCC GACCAGGCAA GAAGCTCAAG AAGGTGAAGC TTCGGAAGTT GGAGAGGCCT 
CCAGAAACGC CAACAGTTGA TCCAACAGTG AAGTATGAGC GACAGCCAGA GTACCTGGAT 
GCTACAGGTG GAACCCTGCA CCCCTATCAA ATGGAGGGCC TGAATTGGTT GCGCTTCTCC 
TGGGCTCAGG GCACTGACAC CATCTTGGCT GATGAGATGG GCCTTGGGAA AACTGTACAG 
ACAGCAGTCT TCCTGTATTC CCTTTACAAG GAGGGTCATT CCAAAGGCCC CTTCCTAGTG 
AGCGCCCCTC TTTCTACCAT CATCAACTGG GAGCGGGAGT TTGAAATGTG GGCTCCAGAC 
ATGTATGTCG TAACCTATGT GGGTGACAAG GACAGCCGTG CCATCATCCG AGAGAATGAG 
TTCTCCTTTG AAGACAATGC CATTCGTGGT GGCAAGAAGG CCTCCCGCAT GAAGAAAGAG 
GCATCTGTGA AATTCCATGT GCTGCTGACA TCCTATGAAT TGATCACCAT TGACATGGCT 
ATTTTGGGCT CTATTGATTG GGCCTGCCTC ATCGTGGATG AAGCCCATCG GCTGAAGAAC 
AATCAGTCTA AGTTCTTCCG GGTATTGAAT GGTTACTCAC TCCAGCACAA GCTGTTGCTG 
ACTGGGACAC CATTACAAAA CAATCTGGAA GAGTTGTTTC ATCTGCTCAA CTTTCTCACC 
CCCGAGAGGT TCCACAATTT GGAAGGTTTT TTGGAGGAGT TTGCTGACAT TGCCAAGGAG 
GACCAGATAA AAAAACTGCA TGACATGCTG GGGCCGCACA TGTTGCGGCG GCTCAAAGCC 
GATGTGTTCA AGAACATGCC CTCCAAGACA GAACTAATTG TGCGTGTGGA GCTGAGCCCT 
ATGCAGAAGA AATACTACAA GTACATCCTC ACTCGAAATT TTGAAGCACT CAATGCCCGA 
GGTGGTGGCA ACCAGGTGTC TCTGCTGAAT GTGGTGATGG ATCTTAAGAA GTGCTGCAAC 
CATCCATACC TCTTCCCTGT GGCTGCAATG GAAGCTCCTA AGATGCCTAA TGGCATGTAT 
GATGGCAGTG CCCTAATCAG AGCATCTGGG AAATTATTGC TGCTGCAGAA AATGCTCAAG 
AACCTTAAGG AGGGTGGGCA TCGTGTACTC ATCTTTTCCC AGATGACCAA GATGCTAGAC 
CTGCTAGAGG ATTTCTTGGA ACATGAAGGT TATAAATACG AACGCATCGA TGGTGGAATC 
ACTGGGAACA TGCGGCAAGA GGCCATTGAC CGCTTCAATG CACCGGGTGC TCAGCAGTTC 
TGCTTCTTGC TTTCCACTCG AGCTGGGGGC CTTGGAATCA ATCTGGCCAC TGCTGACACA 
GTTATTATCT ATGACTCTGA CTGGAACCCC CATAATGACA TTCAGGCCTT TAGCAGAGCT 
CACCGGATTG GGCAAAATAA AAAGGTAATG ATCTACCGGT TTGTGACCCG TGCGTCAGTG 
GAGGAGCGCA TCACGCAGGT GGCAAAGAAG AAAATGATGC TGACGCATCT AGTGGTGCGG 
CCTGGGCTGG GCTCCAAGAC TGGATCTATG TCCAAACAGG AGCTTGATGA TATCCTCAAA 
TTTGGCACTG AGGAACTATT CAAGGATGAA GCCACTGATG GAGGAGGAGA CAACAAAGAG 
GGAGAAGATA GCAGTGTTAT CCACTACGAT GATAAGGCCA TTGAACGGCT GCTAGACCGT 
AACCAGGATG AGACTGAAGA CACAGAATTG CAGGGCATGA ATGAATATTT GAGCTCATTC 
AAAGTGGCCC AGTATGTGGT ACGGGAAGAA GAAATGGGGG AGGAAGAGGA GGTAGAACGG 
GAAATCATTA AACAGGAAGA AAGTGTGGAT CCTGACTACT GGGAGAAATT GCTGCGGCAC 
CATTATGAGC AGCAGCAAGA AGATCTAGCC CGAAATCTGG GCAAAGGAAA AAGAATCCGT 
AAACAGGTCA ACTACAATGA TGGCTCCCAG GAGGACCGAG ATTGGCAGGA CGACCAGTCC 
GACAACCAGT CCGATTACTC AGTGGCTTCA GAGGAAGGTG ATGAAGACTT TGATGAACGT 
TCAGAAGCTC CCCGTAGGCC CAGTCGTAAG GGCCTGCGGA ATGATAAAGA TAAGCCATTG 
CCTCCTCTGT TGGCCCGTGT TGGTGGGAAT ATTGAAGTAC TTGGTTTTAA TGCTCGTCAG 
CGAAAAGCCT TTCTTAATGC AATTATGCGA TATGGTATGC CACCTCAGGA TGCTTTTACT 
ACCCAGTGGC TTGTAAGAGA CCTGCGAGGC AAATCAGAGA AAGAGTTCAA GGCATATGTC 
TCTCTTTTCA TGCGGCATTT ATGTGAGCCG GGGGCAGATG GGGCTGAGAC CTTTGCTGAT 
GGTGTCCCCC GAGAAGGCCT GTCTCGCCAG CATGTCCTTA CTAGAATTGG TGTTATGTCT 
TTGATTCGCA AGAAGGTTCA GGAGTTTGAA CATGTTAATG GGCGCTGGAG CATGCCTGAA 
CTGGCTGAGG TGGAGGAAAA CAAGAAGATG TCCCAGCCAG GGTCACCCTC CCCAAAAACT 
CCTACACCCT CCACTCCAGG GGACACGCAG CCCAACACTC CTGCACCTGT CCCACCTGCT 
GAAGATGGGA TAAAAATAGA GGAAAATAGC CTCAAAGAAG AAGAGAGCAT AGAAGGAGAA 
AAGGAGGTTA AATCTACAGC CCCTGAGACT GCCATTGAGT GTACACAGGC CCCTGCCCCT 
GCCTCAGAGG ATGAAAAGGT CGTTGTTGAA CCCCCTGAGG GAGAGGAGAA AGTGGAAAAG 
GCAGAGGTGA AGGAGAGAAC AGAGGAACCT ATGGAGACAG AGCCCAAAGG TAAAGGTGCT 
GCTGATGTAG AGAAGGTGGA GGAAAAGTCA GCAATAGATC TGACCCCTAT TGTGGTAGAA 
GACAAAGAAG AGAAGAAAGA AGAAGAAGAG AAAAAAGAGG TGATGCTTCA GAATGGAGAG 
ACCCCCAAGG ACCTGAATGA TGAGAAACAG AAGAAAAATA TTAAACAACG TTTCATGTTT 
AACATTGCAG ATGGTGGTTT TACTGAGTTG CACTCCCTTT GGCAGAATGA AGAGCGGGCA 
GCCACAGTTA CCAAGAAGAC TTATGAGATC TGGCATCGAC GGCATGACTA CTGGCTGCTA 
GCCGGCATTA TAAACCATGG CTATGCCCGG TGGCAAGACA TCCAGAATGA CCCACGCTAT 
GCCATCCTCA ATGAGCCTTT CAAGGGTGAA ATGAACCGTG GCAATTTCTT AGAGATCAAG 
AATAAATTTC TAGCTCGAAG GTTTAAGCTC TTAGAACAAG CTCTGGTGAT TGAGGAACAG 
CTGCGCCGGG CTGCTTACTT GAACATGTCA GAAGACCCTT CTCACCCTTC CATGGCCCTC 
AACACCCGCT TTGCTGAGGT GGAGTGTTTG GCGGAAAGTC ATCAGCACCT GTCCAAGGAG 
TCAATGGCAG GAAACAAGCC AGCCAATGCA GTCCTGCACA AAGTTCTGAA ACAGCTGGAA 
GAACTGCTGA GTGACATGAA AGCTGATGTG ACTCGACTCC CAGCTACCAT TGCCCGAATT 
CCCCCAGTTG CTGTGAGGTT ACAGATGTCA GAGCGTAACA TTCTCAGCCG CCTGGCAAAC 
CGGGCACCCG AACCTACCCC ACAGCAGGTA GCCCAGCAGC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016874214.1
CDS	219..5963
Translation	MASGLGSPSPCSAGSEEEDMDALLNNSLPPPHPENEEDPEEDLSETETPKLKKKKKPKKPRDPKIPKSKRQKKERMLLCRQLGDSSGEGPEFVEEEEEVALRSDSEGSDYTPGKKKKKKLGPKKEKKSKSKRKEEEEEEDDDDDSKEPKSSAQLLEDWGMEDIDHVFSEEDYRTLTNYKAFSQFVRPLIAAKNPKIAVSKMMMVLGAKWREFSTNNPFKGSSGASVAAAAAAAVAVVESMVTATEVAPPPPPVEVPIRKAKTKEGKGPNARRKPKGSPRVPDAKKPKPKKVAPLKIKLGGFGSKRKRSSSEDDDLDVESDFDDASINSYSVSDGSTSRSSRSRKKLRTTKKKKKGEEEVTAVDGYETDHQDYCEVCQQGGEIILCDTCPRAYHMVCLDPDMEKAPEGKWSCPHCEKEGIQWEAKEDNSEGEEILEEVGGDLEEEDDHHMEFCRVCKDGGELLCCDTCPSSYHIHCLNPPLPEIPNGEWLCPRCTCPALKGKVQKILIWKWGQPPSPTPVPRPPDADPNTPSPKPLEGRPERQFFVKWQGMSYWHCSWVSELQLELHCQVMFRNYQRKNDMDEPPSGDFGGDEEKSRKRKNKDPKFAEMEERFYRYGIKPEWMMIHRILNHSVDKKGHVHYLIKWRDLPYDQASWESEDVEIQDYDLFKQSYWNHRELMRGEEGRPGKKLKKVKLRKLERPPETPTVDPTVKYERQPEYLDATGGTLHPYQMEGLNWLRFSWAQGTDTILADEMGLGKTVQTAVFLYSLYKEGHSKGPFLVSAPLSTIINWEREFEMWAPDMYVVTYVGDKDSRAIIRENEFSFEDNAIRGGKKASRMKKEASVKFHVLLTSYELITIDMAILGSIDWACLIVDEAHRLKNNQSKFFRVLNGYSLQHKLLLTGTPLQNNLEELFHLLNFLTPERFHNLEGFLEEFADIAKEDQIKKLHDMLGPHMLRRLKADVFKNMPSKTELIVRVELSPMQKKYYKYILTRNFEALNARGGGNQVSLLNVVMDLKKCCNHPYLFPVAAMEAPKMPNGMYDGSALIRASGKLLLLQKMLKNLKEGGHRVLIFSQMTKMLDLLEDFLEHEGYKYERIDGGITGNMRQEAIDRFNAPGAQQFCFLLSTRAGGLGINLATADTVIIYDSDWNPHNDIQAFSRAHRIGQNKKVMIYRFVTRASVEERITQVAKKKMMLTHLVVRPGLGSKTGSMSKQELDDILKFGTEELFKDEATDGGGDNKEGEDSSVIHYDDKAIERLLDRNQDETEDTELQGMNEYLSSFKVAQYVVREEEMGEEEEVEREIIKQEESVDPDYWEKLLRHHYEQQQEDLARNLGKGKRIRKQVNYNDGSQEDRDWQDDQSDNQSDYSVASEEGDEDFDERSEAPRRPSRKGLRNDKDKPLPPLLARVGGNIEVLGFNARQRKAFLNAIMRYGMPPQDAFTTQWLVRDLRGKSEKEFKAYVSLFMRHLCEPGADGAETFADGVPREGLSRQHVLTRIGVMSLIRKKVQEFEHVNGRWSMPELAEVEENKKMSQPGSPSPKTPTPSTPGDTQPNTPAPVPPAEDGIKIEENSLKEEESIEGEKEVKSTAPETAIECTQAPAPASEDEKVVVEPPEGEEKVEKAEVKERTEEPMETEPKGKGAADVEKVEEKSAIDLTPIVVEDKEEKKEEEEKKEVMLQNGETPKDLNDEKQKKNIKQRFMFNIADGGFTELHSLWQNEERAATVTKKTYEIWHRRHDYWLLAGIINHGYARWQDIQNDPRYAILNEPFKGEMNRGNFLEIKNKFLARRFKLLEQALVIEEQLRRAAYLNMSEDPSHPSMALNTRFAEVECLAESHQHLSKESMAGNKPANAVLHKVLKQLEELLSDMKADVTRLPATIARIPPVAVRLQMSERNILSRLANRAPEPTPQQVAQQQ

Target ORF information:

RefSeq Version	XM_017018725.1
Organism	Homo sapiens(human)
Definition	Homo sapiens chromodomain helicase DNA binding protein 4 (CHD4), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017018725.1

ORF Insert Sequence:

ATGGCGTCGG GCCTGGGCTC CCCGTCCCCC TGCTCGGCGG GCAGTGAGGA GGAGGATATG 
GATGCACTTT TGAACAACAG CCTGCCCCCA CCCCACCCAG AAAATGAAGA GGACCCAGAA 
GAGGATTTGT CAGAAACAGA GACTCCAAAG CTCAAGAAGA AGAAAAAGCC TAAGAAACCT 
CGGGACCCTA AAATCCCTAA GAGCAAGCGC CAAAAAAAGG AGCGTATGCT CTTATGCCGG 
CAGCTGGGGG ACAGCTCTGG GGAGGGGCCA GAGTTTGTGG AGGAGGAGGA AGAGGTGGCT 
CTGCGCTCAG ACAGTGAGGG CAGCGACTAT ACTCCTGGCA AGAAGAAGAA GAAGAAGCTT 
GGACCTAAGA AAGAGAAGAA GAGCAAATCC AAGCGGAAGG AGGAGGAGGA GGAGGAGGAT 
GATGATGATG ATTCAAAGGA GCCTAAATCA TCTGCTCAGC TCCTGGAAGA CTGGGGCATG 
GAAGACATTG ACCACGTGTT CTCAGAGGAG GATTATCGAA CCCTCACCAA CTACAAGGCC 
TTCAGCCAGT TTGTCAGACC CCTCATTGCT GCCAAAAATC CCAAGATTGC TGTCTCCAAG 
ATGATGATGG TTTTGGGTGC AAAATGGCGG GAGTTCAGTA CCAATAACCC CTTCAAAGGC 
AGTTCTGGGG CATCAGTGGC AGCTGCGGCA GCAGCAGCGG TAGCTGTGGT GGAGAGCATG 
GTGACAGCCA CTGAGGTTGC ACCACCACCT CCCCCTGTGG AGGTGCCTAT CCGCAAGGCC 
AAGACCAAGG AGGGCAAAGG TCCCAATGCT CGGAGGAAGC CCAAGGGCAG CCCTCGTGTA 
CCTGATGCCA AGAAGCCTAA ACCCAAGAAA GTAGCTCCCC TGAAAATCAA GCTGGGAGGT 
TTTGGTTCCA AGCGTAAGAG ATCCTCGAGT GAGGATGATG ACTTAGATGT GGAATCTGAC 
TTCGATGATG CCAGTATCAA TAGCTATTCT GTTTCTGATG GTTCCACCAG CCGTAGTAGC 
CGCAGCCGCA AGAAACTCCG AACCACTAAA AAGAAAAAGA AAGGCGAGGA GGAGGTGACT 
GCTGTGGATG GTTATGAGAC AGACCACCAG GACTATTGCG AGGTGTGCCA GCAAGGCGGT 
GAGATCATCC TGTGTGATAC CTGTCCCCGT GCTTACCACA TGGTCTGCCT GGATCCCGAC 
ATGGAGAAGG CTCCCGAGGG CAAGTGGAGC TGCCCACACT GCGAGAAGGA AGGCATCCAG 
TGGGAAGCTA AAGAGGACAA TTCGGAGGGT GAGGAGATCC TGGAAGAGGT TGGGGGAGAC 
CTCGAAGAGG AGGATGACCA CCATATGGAA TTCTGTCGGG TCTGCAAGGA TGGTGGGGAA 
CTGCTCTGCT GTGATACCTG TCCTTCTTCC TACCACATCC ACTGCCTGAA TCCCCCACTT 
CCAGAGATCC CCAACGGTGA ATGGCTCTGT CCCCGTTGTA CGTGTCCAGC TCTGAAGGGC 
AAAGTGCAGA AGATCCTAAT CTGGAAGTGG GGTCAGCCAC CATCTCCCAC ACCAGTGCCT 
CGGCCTCCAG ATGCTGATCC CAACACGCCC TCCCCAAAGC CCTTGGAGGG GCGGCCAGAG 
CGGCAGTTCT TTGTGAAATG GCAAGGCATG TCTTACTGGC ACTGCTCCTG GGTTTCTGAA 
CTGCAGCTGG AGCTGCACTG TCAGGTGATG TTCCGAAACT ATCAGCGGAA GAATGATATG 
GATGAGCCAC CTTCTGGGGA CTTTGGTGGT GATGAAGAGA AAAGCCGAAA GCGAAAGAAC 
AAGGACCCTA AATTTGCAGA GATGGAGGAA CGCTTCTATC GCTATGGGAT AAAACCCGAG 
TGGATGATGA TCCACCGAAT CCTCAACCAC AGTGTGGACA AGAAGGGCCA CGTCCACTAC 
TTGATCAAGT GGCGGGACTT ACCTTACGAT CAGGCTTCTT GGGAGAGTGA GGATGTGGAG 
ATCCAGGATT ACGACCTGTT CAAGCAGAGC TATTGGAATC ACAGGGAGTT AATGAGGGGT 
GAGGAAGGCC GACCAGGCAA GAAGCTCAAG AAGGTGAAGC TTCGGAAGTT GGAGAGGCCT 
CCAGAAACGC CAACAGTTGA TCCAACAGTG AAGTATGAGC GACAGCCAGA GTACCTGGAT 
GCTACAGGTG GAACCCTGCA CCCCTATCAA ATGGAGGGCC TGAATTGGTT GCGCTTCTCC 
TGGGCTCAGG GCACTGACAC CATCTTGGCT GATGAGATGG GCCTTGGGAA AACTGTACAG 
ACAGCAGTCT TCCTGTATTC CCTTTACAAG GAGGGTCATT CCAAAGGCCC CTTCCTAGTG 
AGCGCCCCTC TTTCTACCAT CATCAACTGG GAGCGGGAGT TTGAAATGTG GGCTCCAGAC 
ATGTATGTCG TAACCTATGT GGGTGACAAG GACAGCCGTG CCATCATCCG AGAGAATGAG 
TTCTCCTTTG AAGACAATGC CATTCGTGGT GGCAAGAAGG CCTCCCGCAT GAAGAAAGAG 
GCATCTGTGA AATTCCATGT GCTGCTGACA TCCTATGAAT TGATCACCAT TGACATGGCT 
ATTTTGGGCT CTATTGATTG GGCCTGCCTC ATCGTGGATG AAGCCCATCG GCTGAAGAAC 
AATCAGTCTA AGTTCTTCCG GGTATTGAAT GGTTACTCAC TCCAGCACAA GCTGTTGCTG 
ACTGGGACAC CATTACAAAA CAATCTGGAA GAGTTGTTTC ATCTGCTCAA CTTTCTCACC 
CCCGAGAGGT TCCACAATTT GGAAGGTTTT TTGGAGGAGT TTGCTGACAT TGCCAAGGAG 
GACCAGATAA AAAAACTGCA TGACATGCTG GGGCCGCACA TGTTGCGGCG GCTCAAAGCC 
GATGTGTTCA AGAACATGCC CTCCAAGACA GAACTAATTG TGCGTGTGGA GCTGAGCCCT 
ATGCAGAAGA AATACTACAA GTACATCCTC ACTCGAAATT TTGAAGCACT CAATGCCCGA 
GGTGGTGGCA ACCAGGTGTC TCTGCTGAAT GTGGTGATGG ATCTTAAGAA GTGCTGCAAC 
CATCCATACC TCTTCCCTGT GGCTGCAATG GAAGCTCCTA AGATGCCTAA TGGCATGTAT 
GATGGCAGTG CCCTAATCAG AGCATCTGGG AAATTATTGC TGCTGCAGAA AATGCTCAAG 
AACCTTAAGG AGGGTGGGCA TCGTGTACTC ATCTTTTCCC AGATGACCAA GATGCTAGAC 
CTGCTAGAGG ATTTCTTGGA ACATGAAGGT TATAAATACG AACGCATCGA TGGTGGAATC 
ACTGGGAACA TGCGGCAAGA GGCCATTGAC CGCTTCAATG CACCGGGTGC TCAGCAGTTC 
TGCTTCTTGC TTTCCACTCG AGCTGGGGGC CTTGGAATCA ATCTGGCCAC TGCTGACACA 
GTTATTATCT ATGACTCTGA CTGGAACCCC CATAATGACA TTCAGGCCTT TAGCAGAGCT 
CACCGGATTG GGCAAAATAA AAAGGTAATG ATCTACCGGT TTGTGACCCG TGCGTCAGTG 
GAGGAGCGCA TCACGCAGGT GGCAAAGAAG AAAATGATGC TGACGCATCT AGTGGTGCGG 
CCTGGGCTGG GCTCCAAGAC TGGATCTATG TCCAAACAGG AGCTTGATGA TATCCTCAAA 
TTTGGCACTG AGGAACTATT CAAGGATGAA GCCACTGATG GAGGAGGAGA CAACAAAGAG 
GGAGAAGATA GCAGTGTTAT CCACTACGAT GATAAGGCCA TTGAACGGCT GCTAGACCGT 
AACCAGGATG AGACTGAAGA CACAGAATTG CAGGGCATGA ATGAATATTT GAGCTCATTC 
AAAGTGGCCC AGTATGTGGT ACGGGAAGAA GAAATGGGGG AGGAAGAGGA GGTAGAACGG 
GAAATCATTA AACAGGAAGA AAGTGTGGAT CCTGACTACT GGGAGAAATT GCTGCGGCAC 
CATTATGAGC AGCAGCAAGA AGATCTAGCC CGAAATCTGG GCAAAGGAAA AAGAATCCGT 
AAACAGGTCA ACTACAATGA TGGCTCCCAG GAGGACCGAG ATTGGCAGGA CGACCAGTCC 
GACAACCAGT CCGATTACTC AGTGGCTTCA GAGGAAGGTG ATGAAGACTT TGATGAACGT 
TCAGAAGCTC CCCGTAGGCC CAGTCGTAAG GGCCTGCGGA ATGATAAAGA TAAGCCATTG 
CCTCCTCTGT TGGCCCGTGT TGGTGGGAAT ATTGAAGTAC TTGGTTTTAA TGCTCGTCAG 
CGAAAAGCCT TTCTTAATGC AATTATGCGA TATGGTATGC CACCTCAGGA TGCTTTTACT 
ACCCAGTGGC TTGTAAGAGA CCTGCGAGGC AAATCAGAGA AAGAGTTCAA GGCATATGTC 
TCTCTTTTCA TGCGGCATTT ATGTGAGCCG GGGGCAGATG GGGCTGAGAC CTTTGCTGAT 
GGTGTCCCCC GAGAAGGCCT GTCTCGCCAG CATGTCCTTA CTAGAATTGG TGTTATGTCT 
TTGATTCGCA AGAAGGTTCA GGAGTTTGAA CATGTTAATG GGCGCTGGAG CATGCCTGAA 
CTGGCTGAGG TGGAGGAAAA CAAGAAGATG TCCCAGCCAG GGTCACCCTC CCCAAAAACT 
CCTACACCCT CCACTCCAGG GGACACGCAG CCCAACACTC CTGCACCTGT CCCACCTGCT 
GAAGATGGGA TAAAAATAGA GGAAAATAGC CTCAAAGAAG AAGAGAGCAT AGAAGGAGAA 
AAGGAGGTTA AATCTACAGC CCCTGAGACT GCCATTGAGT GTACACAGGC CCCTGCCCCT 
GCCTCAGAGG ATGAAAAGGT CGTTGTTGAA CCCCCTGAGG GAGAGGAGAA AGTGGAAAAG 
GCAGAGGTGA AGGAGAGAAC AGAGGAACCT ATGGAGACAG AGCCCAAAGG TAAAGGTGCT 
GCTGATGTAG AGAAGGTGGA GGAAAAGTCA GCAATAGATC TGACCCCTAT TGTGGTAGAA 
GACAAAGAAG AGAAGAAAGA AGAAGAAGAG AAAAAAGAGG TGATGCTTCA GAATGGAGAG 
ACCCCCAAGG ACCTGAATGA TGAGAAACAG AAGAAAAATA TTAAACAACG TTTCATGTTT 
AACATTGCAG ATGGTGGTTT TACTGAGTTG CACTCCCTTT GGCAGAATGA AGAGCGGGCA 
GCCACAGTTA CCAAGAAGAC TTATGAGATC TGGCATCGAC GGCATGACTA CTGGCTGCTA 
GCCGGCATTA TAAACCATGG CTATGCCCGG TGGCAAGACA TCCAGAATGA CCCACGCTAT 
GCCATCCTCA ATGAGCCTTT CAAGGGTGAA ATGAACCGTG GCAATTTCTT AGAGATCAAG 
AATAAATTTC TAGCTCGAAG GTTTAAGCTC TTAGAACAAG CTCTGGTGAT TGAGGAACAG 
CTGCGCCGGG CTGCTTACTT GAACATGTCA GAAGACCCTT CTCACCCTTC CATGGCCCTC 
AACACCCGCT TTGCTGAGGT GGAGTGTTTG GCGGAAAGTC ATCAGCACCT GTCCAAGGAG 
TCAATGGCAG GAAACAAGCC AGCCAATGCA GTCCTGCACA AAGTTCTGAA ACAGCTGGAA 
GAACTGCTGA GTGACATGAA AGCTGATGTG ACTCGACTCC CAGCTACCAT TGCCCGAATT 
CCCCCAGTTG CTGTGAGGTT ACAGATGTCA GAGCGTAACA TTCTCAGCCG CCTGGCAAAC 
CGGGCACCCG AACCTACCCC ACAGCAGGTA GCCCAGCAGC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHD4 ( XM_017018726.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016874215.1 Homo sapiens chromodomain helicase DNA binding protein 4 (CHD4), transcript variant X9, mRNA.

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CloneID	OHu83471
Clone ID Related Accession (Same CDS sequence)	XM_017018726.1
Accession Version	XM_017018726.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5742bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-25
Organism	Homo sapiens(human)
Product	chromodomain-helicase-DNA-binding protein 4 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCGG GCCTGGGCTC CCCGTCCCCC TGCTCGGCGG GCAGTGAGGA GGAGGATATG 
GATGCACTTT TGAACAACAG CCTGCCCCCA CCCCACCCAG AAAATGAAGA GGACCCAGAA 
GAGGATTTGT CAGAAACAGA GACTCCAAAG CTCAAGAAGA AGAAAAAGCC TAAGAAACCT 
CGGGACCCTA AAATCCCTAA GAGCAAGCGC CAAAAAAAGG AGCGTATGCT CTTATGCCGG 
CAGCTGGGGG ACAGCTCTGG GGAGGGGCCA GAGTTTGTGG AGGAGGAGGA AGAGGTGGCT 
CTGCGCTCAG ACAGTGAGGG CAGCGACTAT ACTCCTGGCA AGAAGAAGAA GAAGAAGCTT 
GGACCTAAGA AAGAGAAGAA GAGCAAATCC AAGCGGAAGG AGGAGGAGGA GGAGGAGGAT 
GATGATGATG ATTCAAAGGA GCCTAAATCA TCTGCTCAGC TCCTGGAAGA CTGGGGCATG 
GAAGACATTG ACCACGTGTT CTCAGAGGAG GATTATCGAA CCCTCACCAA CTACAAGGCC 
TTCAGCCAGT TTGTCAGACC CCTCATTGCT GCCAAAAATC CCAAGATTGC TGTCTCCAAG 
ATGATGATGG TTTTGGGTGC AAAATGGCGG GAGTTCAGTA CCAATAACCC CTTCAAAGGC 
AGTTCTGGGG CATCAGTGGC AGCTGCGGCA GCAGCAGCGG TAGCTGTGGT GGAGAGCATG 
GTGACAGCCA CTGAGGTTGC ACCACCACCT CCCCCTGTGG AGGTGCCTAT CCGCAAGGCC 
AAGACCAAGG AGGGCAAAGG TCCCAATGCT CGGAGGAAGC CCAAGGGCAG CCCTCGTGTA 
CCTGATGCCA AGAAGCCTAA ACCCAAGAAA GTAGCTCCCC TGAAAATCAA GCTGGGAGGT 
TTTGGTTCCA AGCGTAAGAG ATCCTCGAGT GAGGATGATG ACTTAGATGT GGAATCTGAC 
TTCGATGATG CCAGTATCAA TAGCTATTCT GTTTCTGATG GTTCCACCAG CCGTAGTAGC 
CGCAGCCGCA AGAAACTCCG AACCACTAAA AAGAAAAAGA AAGGCGAGGA GGAGGTGACT 
GCTGTGGATG GTTATGAGAC AGACCACCAG GACTATTGCG AGGTGTGCCA GCAAGGCGGT 
GAGATCATCC TGTGTGATAC CTGTCCCCGT GCTTACCACA TGGTCTGCCT GGATCCCGAC 
ATGGAGAAGG CTCCCGAGGG CAAGTGGAGC TGCCCACACT GCGAGAAGGA AGGCATCCAG 
TGGGAAGCTA AAGAGGACAA TTCGGAGGGT GAGGAGATCC TGGAAGAGGT TGGGGGAGAC 
CTCGAAGAGG AGGATGACCA CCATATGGAA TTCTGTCGGG TCTGCAAGGA TGGTGGGGAA 
CTGCTCTGCT GTGATACCTG TCCTTCTTCC TACCACATCC ACTGCCTGAA TCCCCCACTT 
CCAGAGATCC CCAACGGTGA ATGGCTCTGT CCCCGTTGTA CGTGTCCAGC TCTGAAGGGC 
AAAGTGCAGA AGATCCTAAT CTGGAAGTGG GGTCAGCCAC CATCTCCCAC ACCAGTGCCT 
CGGCCTCCAG ATGCTGATCC CAACACGCCC TCCCCAAAGC CCTTGGAGGG GCGGCCAGAG 
CGGCAGTTCT TTGTGAAATG GCAAGGCATG TCTTACTGGC ACTGCTCCTG GGTTTCTGAA 
CTGCAGCTGG AGCTGCACTG TCAGGTGATG TTCCGAAACT ATCAGCGGAA GAATGATATG 
GATGAGCCAC CTTCTGGGGA CTTTGGTGGT GATGAAGAGA AAAGCCGAAA GCGAAAGAAC 
AAGGACCCTA AATTTGCAGA GATGGAGGAA CGCTTCTATC GCTATGGGAT AAAACCCGAG 
TGGATGATGA TCCACCGAAT CCTCAACCAC AGTGTGGACA AGAAGGGCCA CGTCCACTAC 
TTGATCAAGT GGCGGGACTT ACCTTACGAT CAGGCTTCTT GGGAGAGTGA GGATGTGGAG 
ATCCAGGATT ACGACCTGTT CAAGCAGAGC TATTGGAATC ACAGGGAGTT AATGAGGGGT 
GAGGAAGGCC GACCAGGCAA GAAGCTCAAG AAGGTGAAGC TTCGGAAGTT GGAGAGGCCT 
CCAGAAACGC CAACAGTTGA TCCAACAGTG AAGTATGAGC GACAGCCAGA GTACCTGGAT 
GCTACAGGTG GAACCCTGCA CCCCTATCAA ATGGAGGGCC TGAATTGGTT GCGCTTCTCC 
TGGGCTCAGG GCACTGACAC CATCTTGGCT GATGAGATGG GCCTTGGGAA AACTGTACAG 
ACAGCAGTCT TCCTGTATTC CCTTTACAAG GAGGGTCATT CCAAAGGCCC CTTCCTAGTG 
AGCGCCCCTC TTTCTACCAT CATCAACTGG GAGCGGGAGT TTGAAATGTG GGCTCCAGAC 
ATGTATGTCG TAACCTATGT GGGTGACAAG GACAGCCGTG CCATCATCCG AGAGAATGAG 
TTCTCCTTTG AAGACAATGC CATTCGTGGT GGCAAGAAGG CCTCCCGCAT GAAGAAAGAG 
GCATCTGTGA AATTCCATGT GCTGCTGACA TCCTATGAAT TGATCACCAT TGACATGGCT 
ATTTTGGGCT CTATTGATTG GGCCTGCCTC ATCGTGGATG AAGCCCATCG GCTGAAGAAC 
AATCAGTCTA AGTTCTTCCG GGTATTGAAT GGTTACTCAC TCCAGCACAA GCTGTTGCTG 
ACTGGGACAC CATTACAAAA CAATCTGGAA GAGTTGTTTC ATCTGCTCAA CTTTCTCACC 
CCCGAGAGGT TCCACAATTT GGAAGGTTTT TTGGAGGAGT TTGCTGACAT TGCCAAGGAG 
GACCAGATAA AAAAACTGCA TGACATGCTG GGGCCGCACA TGTTGCGGCG GCTCAAAGCC 
GATGTGTTCA AGAACATGCC CTCCAAGACA GAACTAATTG TGCGTGTGGA GCTGAGCCCT 
ATGCAGAAGA AATACTACAA GTACATCCTC ACTCGAAATT TTGAAGCACT CAATGCCCGA 
GGTGGTGGCA ACCAGGTGTC TCTGCTGAAT GTGGTGATGG ATCTTAAGAA GTGCTGCAAC 
CATCCATACC TCTTCCCTGT GGCTGCAATG GAAGCTCCTA AGATGCCTAA TGGCATGTAT 
GATGGCAGTG CCCTAATCAG AGCATCTGGG AAATTATTGC TGCTGCAGAA AATGCTCAAG 
AACCTTAAGG AGGGTGGGCA TCGTGTACTC ATCTTTTCCC AGATGACCAA GATGCTAGAC 
CTGCTAGAGG ATTTCTTGGA ACATGAAGGT TATAAATACG AACGCATCGA TGGTGGAATC 
ACTGGGAACA TGCGGCAAGA GGCCATTGAC CGCTTCAATG CACCGGGTGC TCAGCAGTTC 
TGCTTCTTGC TTTCCACTCG AGCTGGGGGC CTTGGAATCA ATCTGGCCAC TGCTGACACA 
GTTATTATCT ATGACTCTGA CTGGAACCCC CATAATGACA TTCAGGCCTT TAGCAGAGCT 
CACCGGATTG GGCAAAATAA AAAGGTAATG ATCTACCGGT TTGTGACCCG TGCGTCAGTG 
GAGGAGCGCA TCACGCAGGT GGCAAAGAAG AAAATGATGC TGACGCATCT AGTGGTGCGG 
CCTGGGCTGG GCTCCAAGAC TGGATCTATG TCCAAACAGG AGCTTGATGA TATCCTCAAA 
TTTGGCACTG AGGAACTATT CAAGGATGAA GCCACTGATG GAGGAGGAGA CAACAAAGAG 
GGAGAAGATA GCAGTGTTAT CCACTACGAT GATAAGGCCA TTGAACGGCT GCTAGACCGT 
AACCAGGATG AGACTGAAGA CACAGAATTG CAGGGCATGA ATGAATATTT GAGCTCATTC 
AAAGTGGCCC AGTATGTGGT ACGGGAAGAA GAAATGGGGG AGGAAGAGGA GGTAGAACGG 
GAAATCATTA AACAGGAAGA AAGTGTGGAT CCTGACTACT GGGAGAAATT GCTGCGGCAC 
CATTATGAGC AGCAGCAAGA AGATCTAGCC CGAAATCTGG GCAAAGGAAA AAGAATCCGT 
AAACAGGTCA ACTACAATGA TGGCTCCCAG GAGGACCGAG ATTGGCAGGA CGACCAGTCC 
GACAACCAGT CCGATTACTC AGTGGCTTCA GAGGAAGGTG ATGAAGACTT TGATGAACGT 
TCAGAAGCTC CCCGTAGGCC CAGTCGTAAG GGCCTGCGGA ATGATAAAGA TAAGCCATTG 
CCTCCTCTGT TGGCCCGTGT TGGTGGGAAT ATTGAAGTAC TTGGTTTTAA TGCTCGTCAG 
CGAAAAGCCT TTCTTAATGC AATTATGCGA TATGGTATGC CACCTCAGGA TGCTTTTACT 
ACCCAGTGGC TTGTAAGAGA CCTGCGAGGC AAATCAGAGA AAGAGTTCAA GGCATATGTC 
TCTCTTTTCA TGCGGCATTT ATGTGAGCCG GGGGCAGATG GGGCTGAGAC CTTTGCTGAT 
GGTGTCCCCC GAGAAGGCCT GTCTCGCCAG CATGTCCTTA CTAGAATTGG TGTTATGTCT 
TTGATTCGCA AGAAGGTTCA GGAGTTTGAA CATGTTAATG GGCGCTGGAG CATGCCTGAA 
CTGGCTGAGG TGGAGGAAAA CAAGAAGATG TCCCAGCCAG GGTCACCCTC CCCAAAAACT 
CCTACACCCT CCACTCCAGG GGACACGCAG CCCAACACTC CTGCACCTGT CCCACCTGCT 
GAAGATGGGA TAAAAATAGA GGAAAATAGC CTCAAAGAAG AAGAGAGCAT AGAAGGAGAA 
AAGGAGGTTA AATCTACAGC CCCTGAGACT GCCATTGAGT GTACACAGGC CCCTGCCCCT 
GCCTCAGAGG ATGAAAAGGT CGTTGTTGAA CCCCCTGAGG GAGAGGAGAA AGTGGAAAAG 
GCAGAGGTGA AGGAGAGAAC AGAGGAACCT ATGGAGACAG AGCCCAAAGG TGCTGCTGAT 
GTAGAGAAGG TGGAGGAAAA GTCAGCAATA GATCTGACCC CTATTGTGGT AGAAGACAAA 
GAAGAGAAGA AAGAAGAAGA AGAGAAAAAA GAGGTGATGC TTCAGAATGG AGAGACCCCC 
AAGGACCTGA ATGATGAGAA ACAGAAGAAA AATATTAAAC AACGTTTCAT GTTTAACATT 
GCAGATGGTG GTTTTACTGA GTTGCACTCC CTTTGGCAGA ATGAAGAGCG GGCAGCCACA 
GTTACCAAGA AGACTTATGA GATCTGGCAT CGACGGCATG ACTACTGGCT GCTAGCCGGC 
ATTATAAACC ATGGCTATGC CCGGTGGCAA GACATCCAGA ATGACCCACG CTATGCCATC 
CTCAATGAGC CTTTCAAGGG TGAAATGAAC CGTGGCAATT TCTTAGAGAT CAAGAATAAA 
TTTCTAGCTC GAAGGTTTAA GCTCTTAGAA CAAGCTCTGG TGATTGAGGA ACAGCTGCGC 
CGGGCTGCTT ACTTGAACAT GTCAGAAGAC CCTTCTCACC CTTCCATGGC CCTCAACACC 
CGCTTTGCTG AGGTGGAGTG TTTGGCGGAA AGTCATCAGC ACCTGTCCAA GGAGTCAATG 
GCAGGAAACA AGCCAGCCAA TGCAGTCCTG CACAAAGGTA TTCTGAAACA GCTGGAAGAA 
CTGCTGAGTG ACATGAAAGC TGATGTGACT CGACTCCCAG CTACCATTGC CCGAATTCCC 
CCAGTTGCTG TGAGGTTACA GATGTCAGAG CGTAACATTC TCAGCCGCCT GGCAAACCGG 
GCACCCGAAC CTACCCCACA GCAGGTAGCC CAGCAGCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016874215.1
CDS	219..5960
Translation	MASGLGSPSPCSAGSEEEDMDALLNNSLPPPHPENEEDPEEDLSETETPKLKKKKKPKKPRDPKIPKSKRQKKERMLLCRQLGDSSGEGPEFVEEEEEVALRSDSEGSDYTPGKKKKKKLGPKKEKKSKSKRKEEEEEEDDDDDSKEPKSSAQLLEDWGMEDIDHVFSEEDYRTLTNYKAFSQFVRPLIAAKNPKIAVSKMMMVLGAKWREFSTNNPFKGSSGASVAAAAAAAVAVVESMVTATEVAPPPPPVEVPIRKAKTKEGKGPNARRKPKGSPRVPDAKKPKPKKVAPLKIKLGGFGSKRKRSSSEDDDLDVESDFDDASINSYSVSDGSTSRSSRSRKKLRTTKKKKKGEEEVTAVDGYETDHQDYCEVCQQGGEIILCDTCPRAYHMVCLDPDMEKAPEGKWSCPHCEKEGIQWEAKEDNSEGEEILEEVGGDLEEEDDHHMEFCRVCKDGGELLCCDTCPSSYHIHCLNPPLPEIPNGEWLCPRCTCPALKGKVQKILIWKWGQPPSPTPVPRPPDADPNTPSPKPLEGRPERQFFVKWQGMSYWHCSWVSELQLELHCQVMFRNYQRKNDMDEPPSGDFGGDEEKSRKRKNKDPKFAEMEERFYRYGIKPEWMMIHRILNHSVDKKGHVHYLIKWRDLPYDQASWESEDVEIQDYDLFKQSYWNHRELMRGEEGRPGKKLKKVKLRKLERPPETPTVDPTVKYERQPEYLDATGGTLHPYQMEGLNWLRFSWAQGTDTILADEMGLGKTVQTAVFLYSLYKEGHSKGPFLVSAPLSTIINWEREFEMWAPDMYVVTYVGDKDSRAIIRENEFSFEDNAIRGGKKASRMKKEASVKFHVLLTSYELITIDMAILGSIDWACLIVDEAHRLKNNQSKFFRVLNGYSLQHKLLLTGTPLQNNLEELFHLLNFLTPERFHNLEGFLEEFADIAKEDQIKKLHDMLGPHMLRRLKADVFKNMPSKTELIVRVELSPMQKKYYKYILTRNFEALNARGGGNQVSLLNVVMDLKKCCNHPYLFPVAAMEAPKMPNGMYDGSALIRASGKLLLLQKMLKNLKEGGHRVLIFSQMTKMLDLLEDFLEHEGYKYERIDGGITGNMRQEAIDRFNAPGAQQFCFLLSTRAGGLGINLATADTVIIYDSDWNPHNDIQAFSRAHRIGQNKKVMIYRFVTRASVEERITQVAKKKMMLTHLVVRPGLGSKTGSMSKQELDDILKFGTEELFKDEATDGGGDNKEGEDSSVIHYDDKAIERLLDRNQDETEDTELQGMNEYLSSFKVAQYVVREEEMGEEEEVEREIIKQEESVDPDYWEKLLRHHYEQQQEDLARNLGKGKRIRKQVNYNDGSQEDRDWQDDQSDNQSDYSVASEEGDEDFDERSEAPRRPSRKGLRNDKDKPLPPLLARVGGNIEVLGFNARQRKAFLNAIMRYGMPPQDAFTTQWLVRDLRGKSEKEFKAYVSLFMRHLCEPGADGAETFADGVPREGLSRQHVLTRIGVMSLIRKKVQEFEHVNGRWSMPELAEVEENKKMSQPGSPSPKTPTPSTPGDTQPNTPAPVPPAEDGIKIEENSLKEEESIEGEKEVKSTAPETAIECTQAPAPASEDEKVVVEPPEGEEKVEKAEVKERTEEPMETEPKGAADVEKVEEKSAIDLTPIVVEDKEEKKEEEEKKEVMLQNGETPKDLNDEKQKKNIKQRFMFNIADGGFTELHSLWQNEERAATVTKKTYEIWHRRHDYWLLAGIINHGYARWQDIQNDPRYAILNEPFKGEMNRGNFLEIKNKFLARRFKLLEQALVIEEQLRRAAYLNMSEDPSHPSMALNTRFAEVECLAESHQHLSKESMAGNKPANAVLHKGILKQLEELLSDMKADVTRLPATIARIPPVAVRLQMSERNILSRLANRAPEPTPQQVAQQQ

Target ORF information:

RefSeq Version	XM_017018726.1
Organism	Homo sapiens(human)
Definition	Homo sapiens chromodomain helicase DNA binding protein 4 (CHD4), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017018726.1

ORF Insert Sequence:

ATGGCGTCGG GCCTGGGCTC CCCGTCCCCC TGCTCGGCGG GCAGTGAGGA GGAGGATATG 
GATGCACTTT TGAACAACAG CCTGCCCCCA CCCCACCCAG AAAATGAAGA GGACCCAGAA 
GAGGATTTGT CAGAAACAGA GACTCCAAAG CTCAAGAAGA AGAAAAAGCC TAAGAAACCT 
CGGGACCCTA AAATCCCTAA GAGCAAGCGC CAAAAAAAGG AGCGTATGCT CTTATGCCGG 
CAGCTGGGGG ACAGCTCTGG GGAGGGGCCA GAGTTTGTGG AGGAGGAGGA AGAGGTGGCT 
CTGCGCTCAG ACAGTGAGGG CAGCGACTAT ACTCCTGGCA AGAAGAAGAA GAAGAAGCTT 
GGACCTAAGA AAGAGAAGAA GAGCAAATCC AAGCGGAAGG AGGAGGAGGA GGAGGAGGAT 
GATGATGATG ATTCAAAGGA GCCTAAATCA TCTGCTCAGC TCCTGGAAGA CTGGGGCATG 
GAAGACATTG ACCACGTGTT CTCAGAGGAG GATTATCGAA CCCTCACCAA CTACAAGGCC 
TTCAGCCAGT TTGTCAGACC CCTCATTGCT GCCAAAAATC CCAAGATTGC TGTCTCCAAG 
ATGATGATGG TTTTGGGTGC AAAATGGCGG GAGTTCAGTA CCAATAACCC CTTCAAAGGC 
AGTTCTGGGG CATCAGTGGC AGCTGCGGCA GCAGCAGCGG TAGCTGTGGT GGAGAGCATG 
GTGACAGCCA CTGAGGTTGC ACCACCACCT CCCCCTGTGG AGGTGCCTAT CCGCAAGGCC 
AAGACCAAGG AGGGCAAAGG TCCCAATGCT CGGAGGAAGC CCAAGGGCAG CCCTCGTGTA 
CCTGATGCCA AGAAGCCTAA ACCCAAGAAA GTAGCTCCCC TGAAAATCAA GCTGGGAGGT 
TTTGGTTCCA AGCGTAAGAG ATCCTCGAGT GAGGATGATG ACTTAGATGT GGAATCTGAC 
TTCGATGATG CCAGTATCAA TAGCTATTCT GTTTCTGATG GTTCCACCAG CCGTAGTAGC 
CGCAGCCGCA AGAAACTCCG AACCACTAAA AAGAAAAAGA AAGGCGAGGA GGAGGTGACT 
GCTGTGGATG GTTATGAGAC AGACCACCAG GACTATTGCG AGGTGTGCCA GCAAGGCGGT 
GAGATCATCC TGTGTGATAC CTGTCCCCGT GCTTACCACA TGGTCTGCCT GGATCCCGAC 
ATGGAGAAGG CTCCCGAGGG CAAGTGGAGC TGCCCACACT GCGAGAAGGA AGGCATCCAG 
TGGGAAGCTA AAGAGGACAA TTCGGAGGGT GAGGAGATCC TGGAAGAGGT TGGGGGAGAC 
CTCGAAGAGG AGGATGACCA CCATATGGAA TTCTGTCGGG TCTGCAAGGA TGGTGGGGAA 
CTGCTCTGCT GTGATACCTG TCCTTCTTCC TACCACATCC ACTGCCTGAA TCCCCCACTT 
CCAGAGATCC CCAACGGTGA ATGGCTCTGT CCCCGTTGTA CGTGTCCAGC TCTGAAGGGC 
AAAGTGCAGA AGATCCTAAT CTGGAAGTGG GGTCAGCCAC CATCTCCCAC ACCAGTGCCT 
CGGCCTCCAG ATGCTGATCC CAACACGCCC TCCCCAAAGC CCTTGGAGGG GCGGCCAGAG 
CGGCAGTTCT TTGTGAAATG GCAAGGCATG TCTTACTGGC ACTGCTCCTG GGTTTCTGAA 
CTGCAGCTGG AGCTGCACTG TCAGGTGATG TTCCGAAACT ATCAGCGGAA GAATGATATG 
GATGAGCCAC CTTCTGGGGA CTTTGGTGGT GATGAAGAGA AAAGCCGAAA GCGAAAGAAC 
AAGGACCCTA AATTTGCAGA GATGGAGGAA CGCTTCTATC GCTATGGGAT AAAACCCGAG 
TGGATGATGA TCCACCGAAT CCTCAACCAC AGTGTGGACA AGAAGGGCCA CGTCCACTAC 
TTGATCAAGT GGCGGGACTT ACCTTACGAT CAGGCTTCTT GGGAGAGTGA GGATGTGGAG 
ATCCAGGATT ACGACCTGTT CAAGCAGAGC TATTGGAATC ACAGGGAGTT AATGAGGGGT 
GAGGAAGGCC GACCAGGCAA GAAGCTCAAG AAGGTGAAGC TTCGGAAGTT GGAGAGGCCT 
CCAGAAACGC CAACAGTTGA TCCAACAGTG AAGTATGAGC GACAGCCAGA GTACCTGGAT 
GCTACAGGTG GAACCCTGCA CCCCTATCAA ATGGAGGGCC TGAATTGGTT GCGCTTCTCC 
TGGGCTCAGG GCACTGACAC CATCTTGGCT GATGAGATGG GCCTTGGGAA AACTGTACAG 
ACAGCAGTCT TCCTGTATTC CCTTTACAAG GAGGGTCATT CCAAAGGCCC CTTCCTAGTG 
AGCGCCCCTC TTTCTACCAT CATCAACTGG GAGCGGGAGT TTGAAATGTG GGCTCCAGAC 
ATGTATGTCG TAACCTATGT GGGTGACAAG GACAGCCGTG CCATCATCCG AGAGAATGAG 
TTCTCCTTTG AAGACAATGC CATTCGTGGT GGCAAGAAGG CCTCCCGCAT GAAGAAAGAG 
GCATCTGTGA AATTCCATGT GCTGCTGACA TCCTATGAAT TGATCACCAT TGACATGGCT 
ATTTTGGGCT CTATTGATTG GGCCTGCCTC ATCGTGGATG AAGCCCATCG GCTGAAGAAC 
AATCAGTCTA AGTTCTTCCG GGTATTGAAT GGTTACTCAC TCCAGCACAA GCTGTTGCTG 
ACTGGGACAC CATTACAAAA CAATCTGGAA GAGTTGTTTC ATCTGCTCAA CTTTCTCACC 
CCCGAGAGGT TCCACAATTT GGAAGGTTTT TTGGAGGAGT TTGCTGACAT TGCCAAGGAG 
GACCAGATAA AAAAACTGCA TGACATGCTG GGGCCGCACA TGTTGCGGCG GCTCAAAGCC 
GATGTGTTCA AGAACATGCC CTCCAAGACA GAACTAATTG TGCGTGTGGA GCTGAGCCCT 
ATGCAGAAGA AATACTACAA GTACATCCTC ACTCGAAATT TTGAAGCACT CAATGCCCGA 
GGTGGTGGCA ACCAGGTGTC TCTGCTGAAT GTGGTGATGG ATCTTAAGAA GTGCTGCAAC 
CATCCATACC TCTTCCCTGT GGCTGCAATG GAAGCTCCTA AGATGCCTAA TGGCATGTAT 
GATGGCAGTG CCCTAATCAG AGCATCTGGG AAATTATTGC TGCTGCAGAA AATGCTCAAG 
AACCTTAAGG AGGGTGGGCA TCGTGTACTC ATCTTTTCCC AGATGACCAA GATGCTAGAC 
CTGCTAGAGG ATTTCTTGGA ACATGAAGGT TATAAATACG AACGCATCGA TGGTGGAATC 
ACTGGGAACA TGCGGCAAGA GGCCATTGAC CGCTTCAATG CACCGGGTGC TCAGCAGTTC 
TGCTTCTTGC TTTCCACTCG AGCTGGGGGC CTTGGAATCA ATCTGGCCAC TGCTGACACA 
GTTATTATCT ATGACTCTGA CTGGAACCCC CATAATGACA TTCAGGCCTT TAGCAGAGCT 
CACCGGATTG GGCAAAATAA AAAGGTAATG ATCTACCGGT TTGTGACCCG TGCGTCAGTG 
GAGGAGCGCA TCACGCAGGT GGCAAAGAAG AAAATGATGC TGACGCATCT AGTGGTGCGG 
CCTGGGCTGG GCTCCAAGAC TGGATCTATG TCCAAACAGG AGCTTGATGA TATCCTCAAA 
TTTGGCACTG AGGAACTATT CAAGGATGAA GCCACTGATG GAGGAGGAGA CAACAAAGAG 
GGAGAAGATA GCAGTGTTAT CCACTACGAT GATAAGGCCA TTGAACGGCT GCTAGACCGT 
AACCAGGATG AGACTGAAGA CACAGAATTG CAGGGCATGA ATGAATATTT GAGCTCATTC 
AAAGTGGCCC AGTATGTGGT ACGGGAAGAA GAAATGGGGG AGGAAGAGGA GGTAGAACGG 
GAAATCATTA AACAGGAAGA AAGTGTGGAT CCTGACTACT GGGAGAAATT GCTGCGGCAC 
CATTATGAGC AGCAGCAAGA AGATCTAGCC CGAAATCTGG GCAAAGGAAA AAGAATCCGT 
AAACAGGTCA ACTACAATGA TGGCTCCCAG GAGGACCGAG ATTGGCAGGA CGACCAGTCC 
GACAACCAGT CCGATTACTC AGTGGCTTCA GAGGAAGGTG ATGAAGACTT TGATGAACGT 
TCAGAAGCTC CCCGTAGGCC CAGTCGTAAG GGCCTGCGGA ATGATAAAGA TAAGCCATTG 
CCTCCTCTGT TGGCCCGTGT TGGTGGGAAT ATTGAAGTAC TTGGTTTTAA TGCTCGTCAG 
CGAAAAGCCT TTCTTAATGC AATTATGCGA TATGGTATGC CACCTCAGGA TGCTTTTACT 
ACCCAGTGGC TTGTAAGAGA CCTGCGAGGC AAATCAGAGA AAGAGTTCAA GGCATATGTC 
TCTCTTTTCA TGCGGCATTT ATGTGAGCCG GGGGCAGATG GGGCTGAGAC CTTTGCTGAT 
GGTGTCCCCC GAGAAGGCCT GTCTCGCCAG CATGTCCTTA CTAGAATTGG TGTTATGTCT 
TTGATTCGCA AGAAGGTTCA GGAGTTTGAA CATGTTAATG GGCGCTGGAG CATGCCTGAA 
CTGGCTGAGG TGGAGGAAAA CAAGAAGATG TCCCAGCCAG GGTCACCCTC CCCAAAAACT 
CCTACACCCT CCACTCCAGG GGACACGCAG CCCAACACTC CTGCACCTGT CCCACCTGCT 
GAAGATGGGA TAAAAATAGA GGAAAATAGC CTCAAAGAAG AAGAGAGCAT AGAAGGAGAA 
AAGGAGGTTA AATCTACAGC CCCTGAGACT GCCATTGAGT GTACACAGGC CCCTGCCCCT 
GCCTCAGAGG ATGAAAAGGT CGTTGTTGAA CCCCCTGAGG GAGAGGAGAA AGTGGAAAAG 
GCAGAGGTGA AGGAGAGAAC AGAGGAACCT ATGGAGACAG AGCCCAAAGG TGCTGCTGAT 
GTAGAGAAGG TGGAGGAAAA GTCAGCAATA GATCTGACCC CTATTGTGGT AGAAGACAAA 
GAAGAGAAGA AAGAAGAAGA AGAGAAAAAA GAGGTGATGC TTCAGAATGG AGAGACCCCC 
AAGGACCTGA ATGATGAGAA ACAGAAGAAA AATATTAAAC AACGTTTCAT GTTTAACATT 
GCAGATGGTG GTTTTACTGA GTTGCACTCC CTTTGGCAGA ATGAAGAGCG GGCAGCCACA 
GTTACCAAGA AGACTTATGA GATCTGGCAT CGACGGCATG ACTACTGGCT GCTAGCCGGC 
ATTATAAACC ATGGCTATGC CCGGTGGCAA GACATCCAGA ATGACCCACG CTATGCCATC 
CTCAATGAGC CTTTCAAGGG TGAAATGAAC CGTGGCAATT TCTTAGAGAT CAAGAATAAA 
TTTCTAGCTC GAAGGTTTAA GCTCTTAGAA CAAGCTCTGG TGATTGAGGA ACAGCTGCGC 
CGGGCTGCTT ACTTGAACAT GTCAGAAGAC CCTTCTCACC CTTCCATGGC CCTCAACACC 
CGCTTTGCTG AGGTGGAGTG TTTGGCGGAA AGTCATCAGC ACCTGTCCAA GGAGTCAATG 
GCAGGAAACA AGCCAGCCAA TGCAGTCCTG CACAAAGGTA TTCTGAAACA GCTGGAAGAA 
CTGCTGAGTG ACATGAAAGC TGATGTGACT CGACTCCCAG CTACCATTGC CCGAATTCCC 
CCAGTTGCTG TGAGGTTACA GATGTCAGAG CGTAACATTC TCAGCCGCCT GGCAAACCGG 
GCACCCGAAC CTACCCCACA GCAGGTAGCC CAGCAGCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHD4 ( XM_017018727.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016874216.1 Homo sapiens chromodomain helicase DNA binding protein 4 (CHD4), transcript variant X10, mRNA.

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CloneID	OHu83472
Clone ID Related Accession (Same CDS sequence)	XM_017018727.1
Accession Version	XM_017018727.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5739bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-25
Organism	Homo sapiens(human)
Product	chromodomain-helicase-DNA-binding protein 4 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCGG GCCTGGGCTC CCCGTCCCCC TGCTCGGCGG GCAGTGAGGA GGAGGATATG 
GATGCACTTT TGAACAACAG CCTGCCCCCA CCCCACCCAG AAAATGAAGA GGACCCAGAA 
GAGGATTTGT CAGAAACAGA GACTCCAAAG CTCAAGAAGA AGAAAAAGCC TAAGAAACCT 
CGGGACCCTA AAATCCCTAA GAGCAAGCGC CAAAAAAAGG AGCGTATGCT CTTATGCCGG 
CAGCTGGGGG ACAGCTCTGG GGAGGGGCCA GAGTTTGTGG AGGAGGAGGA AGAGGTGGCT 
CTGCGCTCAG ACAGTGAGGG CAGCGACTAT ACTCCTGGCA AGAAGAAGAA GAAGAAGCTT 
GGACCTAAGA AAGAGAAGAA GAGCAAATCC AAGCGGAAGG AGGAGGAGGA GGAGGAGGAT 
GATGATGATG ATTCAAAGGA GCCTAAATCA TCTGCTCAGC TCCTGGAAGA CTGGGGCATG 
GAAGACATTG ACCACGTGTT CTCAGAGGAG GATTATCGAA CCCTCACCAA CTACAAGGCC 
TTCAGCCAGT TTGTCAGACC CCTCATTGCT GCCAAAAATC CCAAGATTGC TGTCTCCAAG 
ATGATGATGG TTTTGGGTGC AAAATGGCGG GAGTTCAGTA CCAATAACCC CTTCAAAGGC 
AGTTCTGGGG CATCAGTGGC AGCTGCGGCA GCAGCAGCGG TAGCTGTGGT GGAGAGCATG 
GTGACAGCCA CTGAGGTTGC ACCACCACCT CCCCCTGTGG AGGTGCCTAT CCGCAAGGCC 
AAGACCAAGG AGGGCAAAGG TCCCAATGCT CGGAGGAAGC CCAAGGGCAG CCCTCGTGTA 
CCTGATGCCA AGAAGCCTAA ACCCAAGAAA GTAGCTCCCC TGAAAATCAA GCTGGGAGGT 
TTTGGTTCCA AGCGTAAGAG ATCCTCGAGT GAGGATGATG ACTTAGATGT GGAATCTGAC 
TTCGATGATG CCAGTATCAA TAGCTATTCT GTTTCTGATG GTTCCACCAG CCGTAGTAGC 
CGCAGCCGCA AGAAACTCCG AACCACTAAA AAGAAAAAGA AAGGCGAGGA GGAGGTGACT 
GCTGTGGATG GTTATGAGAC AGACCACCAG GACTATTGCG AGGTGTGCCA GCAAGGCGGT 
GAGATCATCC TGTGTGATAC CTGTCCCCGT GCTTACCACA TGGTCTGCCT GGATCCCGAC 
ATGGAGAAGG CTCCCGAGGG CAAGTGGAGC TGCCCACACT GCGAGAAGGA AGGCATCCAG 
TGGGAAGCTA AAGAGGACAA TTCGGAGGGT GAGGAGATCC TGGAAGAGGT TGGGGGAGAC 
CTCGAAGAGG AGGATGACCA CCATATGGAA TTCTGTCGGG TCTGCAAGGA TGGTGGGGAA 
CTGCTCTGCT GTGATACCTG TCCTTCTTCC TACCACATCC ACTGCCTGAA TCCCCCACTT 
CCAGAGATCC CCAACGGTGA ATGGCTCTGT CCCCGTTGTA CGTGTCCAGC TCTGAAGGGC 
AAAGTGCAGA AGATCCTAAT CTGGAAGTGG GGTCAGCCAC CATCTCCCAC ACCAGTGCCT 
CGGCCTCCAG ATGCTGATCC CAACACGCCC TCCCCAAAGC CCTTGGAGGG GCGGCCAGAG 
CGGCAGTTCT TTGTGAAATG GCAAGGCATG TCTTACTGGC ACTGCTCCTG GGTTTCTGAA 
CTGCAGCTGG AGCTGCACTG TCAGGTGATG TTCCGAAACT ATCAGCGGAA GAATGATATG 
GATGAGCCAC CTTCTGGGGA CTTTGGTGGT GATGAAGAGA AAAGCCGAAA GCGAAAGAAC 
AAGGACCCTA AATTTGCAGA GATGGAGGAA CGCTTCTATC GCTATGGGAT AAAACCCGAG 
TGGATGATGA TCCACCGAAT CCTCAACCAC AGTGTGGACA AGAAGGGCCA CGTCCACTAC 
TTGATCAAGT GGCGGGACTT ACCTTACGAT CAGGCTTCTT GGGAGAGTGA GGATGTGGAG 
ATCCAGGATT ACGACCTGTT CAAGCAGAGC TATTGGAATC ACAGGGAGTT AATGAGGGGT 
GAGGAAGGCC GACCAGGCAA GAAGCTCAAG AAGGTGAAGC TTCGGAAGTT GGAGAGGCCT 
CCAGAAACGC CAACAGTTGA TCCAACAGTG AAGTATGAGC GACAGCCAGA GTACCTGGAT 
GCTACAGGTG GAACCCTGCA CCCCTATCAA ATGGAGGGCC TGAATTGGTT GCGCTTCTCC 
TGGGCTCAGG GCACTGACAC CATCTTGGCT GATGAGATGG GCCTTGGGAA AACTGTACAG 
ACAGCAGTCT TCCTGTATTC CCTTTACAAG GAGGGTCATT CCAAAGGCCC CTTCCTAGTG 
AGCGCCCCTC TTTCTACCAT CATCAACTGG GAGCGGGAGT TTGAAATGTG GGCTCCAGAC 
ATGTATGTCG TAACCTATGT GGGTGACAAG GACAGCCGTG CCATCATCCG AGAGAATGAG 
TTCTCCTTTG AAGACAATGC CATTCGTGGT GGCAAGAAGG CCTCCCGCAT GAAGAAAGAG 
GCATCTGTGA AATTCCATGT GCTGCTGACA TCCTATGAAT TGATCACCAT TGACATGGCT 
ATTTTGGGCT CTATTGATTG GGCCTGCCTC ATCGTGGATG AAGCCCATCG GCTGAAGAAC 
AATCAGTCTA AGTTCTTCCG GGTATTGAAT GGTTACTCAC TCCAGCACAA GCTGTTGCTG 
ACTGGGACAC CATTACAAAA CAATCTGGAA GAGTTGTTTC ATCTGCTCAA CTTTCTCACC 
CCCGAGAGGT TCCACAATTT GGAAGGTTTT TTGGAGGAGT TTGCTGACAT TGCCAAGGAG 
GACCAGATAA AAAAACTGCA TGACATGCTG GGGCCGCACA TGTTGCGGCG GCTCAAAGCC 
GATGTGTTCA AGAACATGCC CTCCAAGACA GAACTAATTG TGCGTGTGGA GCTGAGCCCT 
ATGCAGAAGA AATACTACAA GTACATCCTC ACTCGAAATT TTGAAGCACT CAATGCCCGA 
GGTGGTGGCA ACCAGGTGTC TCTGCTGAAT GTGGTGATGG ATCTTAAGAA GTGCTGCAAC 
CATCCATACC TCTTCCCTGT GGCTGCAATG GAAGCTCCTA AGATGCCTAA TGGCATGTAT 
GATGGCAGTG CCCTAATCAG AGCATCTGGG AAATTATTGC TGCTGCAGAA AATGCTCAAG 
AACCTTAAGG AGGGTGGGCA TCGTGTACTC ATCTTTTCCC AGATGACCAA GATGCTAGAC 
CTGCTAGAGG ATTTCTTGGA ACATGAAGGT TATAAATACG AACGCATCGA TGGTGGAATC 
ACTGGGAACA TGCGGCAAGA GGCCATTGAC CGCTTCAATG CACCGGGTGC TCAGCAGTTC 
TGCTTCTTGC TTTCCACTCG AGCTGGGGGC CTTGGAATCA ATCTGGCCAC TGCTGACACA 
GTTATTATCT ATGACTCTGA CTGGAACCCC CATAATGACA TTCAGGCCTT TAGCAGAGCT 
CACCGGATTG GGCAAAATAA AAAGGTAATG ATCTACCGGT TTGTGACCCG TGCGTCAGTG 
GAGGAGCGCA TCACGCAGGT GGCAAAGAAG AAAATGATGC TGACGCATCT AGTGGTGCGG 
CCTGGGCTGG GCTCCAAGAC TGGATCTATG TCCAAACAGG AGCTTGATGA TATCCTCAAA 
TTTGGCACTG AGGAACTATT CAAGGATGAA GCCACTGATG GAGGAGGAGA CAACAAAGAG 
GGAGAAGATA GCAGTGTTAT CCACTACGAT GATAAGGCCA TTGAACGGCT GCTAGACCGT 
AACCAGGATG AGACTGAAGA CACAGAATTG CAGGGCATGA ATGAATATTT GAGCTCATTC 
AAAGTGGCCC AGTATGTGGT ACGGGAAGAA GAAATGGGGG AAGAGGAGGT AGAACGGGAA 
ATCATTAAAC AGGAAGAAAG TGTGGATCCT GACTACTGGG AGAAATTGCT GCGGCACCAT 
TATGAGCAGC AGCAAGAAGA TCTAGCCCGA AATCTGGGCA AAGGAAAAAG AATCCGTAAA 
CAGGTCAACT ACAATGATGG CTCCCAGGAG GACCGAGATT GGCAGGACGA CCAGTCCGAC 
AACCAGTCCG ATTACTCAGT GGCTTCAGAG GAAGGTGATG AAGACTTTGA TGAACGTTCA 
GAAGCTCCCC GTAGGCCCAG TCGTAAGGGC CTGCGGAATG ATAAAGATAA GCCATTGCCT 
CCTCTGTTGG CCCGTGTTGG TGGGAATATT GAAGTACTTG GTTTTAATGC TCGTCAGCGA 
AAAGCCTTTC TTAATGCAAT TATGCGATAT GGTATGCCAC CTCAGGATGC TTTTACTACC 
CAGTGGCTTG TAAGAGACCT GCGAGGCAAA TCAGAGAAAG AGTTCAAGGC ATATGTCTCT 
CTTTTCATGC GGCATTTATG TGAGCCGGGG GCAGATGGGG CTGAGACCTT TGCTGATGGT 
GTCCCCCGAG AAGGCCTGTC TCGCCAGCAT GTCCTTACTA GAATTGGTGT TATGTCTTTG 
ATTCGCAAGA AGGTTCAGGA GTTTGAACAT GTTAATGGGC GCTGGAGCAT GCCTGAACTG 
GCTGAGGTGG AGGAAAACAA GAAGATGTCC CAGCCAGGGT CACCCTCCCC AAAAACTCCT 
ACACCCTCCA CTCCAGGGGA CACGCAGCCC AACACTCCTG CACCTGTCCC ACCTGCTGAA 
GATGGGATAA AAATAGAGGA AAATAGCCTC AAAGAAGAAG AGAGCATAGA AGGAGAAAAG 
GAGGTTAAAT CTACAGCCCC TGAGACTGCC ATTGAGTGTA CACAGGCCCC TGCCCCTGCC 
TCAGAGGATG AAAAGGTCGT TGTTGAACCC CCTGAGGGAG AGGAGAAAGT GGAAAAGGCA 
GAGGTGAAGG AGAGAACAGA GGAACCTATG GAGACAGAGC CCAAAGGTGC TGCTGATGTA 
GAGAAGGTGG AGGAAAAGTC AGCAATAGAT CTGACCCCTA TTGTGGTAGA AGACAAAGAA 
GAGAAGAAAG AAGAAGAAGA GAAAAAAGAG GTGATGCTTC AGAATGGAGA GACCCCCAAG 
GACCTGAATG ATGAGAAACA GAAGAAAAAT ATTAAACAAC GTTTCATGTT TAACATTGCA 
GATGGTGGTT TTACTGAGTT GCACTCCCTT TGGCAGAATG AAGAGCGGGC AGCCACAGTT 
ACCAAGAAGA CTTATGAGAT CTGGCATCGA CGGCATGACT ACTGGCTGCT AGCCGGCATT 
ATAAACCATG GCTATGCCCG GTGGCAAGAC ATCCAGAATG ACCCACGCTA TGCCATCCTC 
AATGAGCCTT TCAAGGGTGA AATGAACCGT GGCAATTTCT TAGAGATCAA GAATAAATTT 
CTAGCTCGAA GGTTTAAGCT CTTAGAACAA GCTCTGGTGA TTGAGGAACA GCTGCGCCGG 
GCTGCTTACT TGAACATGTC AGAAGACCCT TCTCACCCTT CCATGGCCCT CAACACCCGC 
TTTGCTGAGG TGGAGTGTTT GGCGGAAAGT CATCAGCACC TGTCCAAGGA GTCAATGGCA 
GGAAACAAGC CAGCCAATGC AGTCCTGCAC AAAGGTATTC TGAAACAGCT GGAAGAACTG 
CTGAGTGACA TGAAAGCTGA TGTGACTCGA CTCCCAGCTA CCATTGCCCG AATTCCCCCA 
GTTGCTGTGA GGTTACAGAT GTCAGAGCGT AACATTCTCA GCCGCCTGGC AAACCGGGCA 
CCCGAACCTA CCCCACAGCA GGTAGCCCAG CAGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016874216.1
CDS	219..5957
Translation	MASGLGSPSPCSAGSEEEDMDALLNNSLPPPHPENEEDPEEDLSETETPKLKKKKKPKKPRDPKIPKSKRQKKERMLLCRQLGDSSGEGPEFVEEEEEVALRSDSEGSDYTPGKKKKKKLGPKKEKKSKSKRKEEEEEEDDDDDSKEPKSSAQLLEDWGMEDIDHVFSEEDYRTLTNYKAFSQFVRPLIAAKNPKIAVSKMMMVLGAKWREFSTNNPFKGSSGASVAAAAAAAVAVVESMVTATEVAPPPPPVEVPIRKAKTKEGKGPNARRKPKGSPRVPDAKKPKPKKVAPLKIKLGGFGSKRKRSSSEDDDLDVESDFDDASINSYSVSDGSTSRSSRSRKKLRTTKKKKKGEEEVTAVDGYETDHQDYCEVCQQGGEIILCDTCPRAYHMVCLDPDMEKAPEGKWSCPHCEKEGIQWEAKEDNSEGEEILEEVGGDLEEEDDHHMEFCRVCKDGGELLCCDTCPSSYHIHCLNPPLPEIPNGEWLCPRCTCPALKGKVQKILIWKWGQPPSPTPVPRPPDADPNTPSPKPLEGRPERQFFVKWQGMSYWHCSWVSELQLELHCQVMFRNYQRKNDMDEPPSGDFGGDEEKSRKRKNKDPKFAEMEERFYRYGIKPEWMMIHRILNHSVDKKGHVHYLIKWRDLPYDQASWESEDVEIQDYDLFKQSYWNHRELMRGEEGRPGKKLKKVKLRKLERPPETPTVDPTVKYERQPEYLDATGGTLHPYQMEGLNWLRFSWAQGTDTILADEMGLGKTVQTAVFLYSLYKEGHSKGPFLVSAPLSTIINWEREFEMWAPDMYVVTYVGDKDSRAIIRENEFSFEDNAIRGGKKASRMKKEASVKFHVLLTSYELITIDMAILGSIDWACLIVDEAHRLKNNQSKFFRVLNGYSLQHKLLLTGTPLQNNLEELFHLLNFLTPERFHNLEGFLEEFADIAKEDQIKKLHDMLGPHMLRRLKADVFKNMPSKTELIVRVELSPMQKKYYKYILTRNFEALNARGGGNQVSLLNVVMDLKKCCNHPYLFPVAAMEAPKMPNGMYDGSALIRASGKLLLLQKMLKNLKEGGHRVLIFSQMTKMLDLLEDFLEHEGYKYERIDGGITGNMRQEAIDRFNAPGAQQFCFLLSTRAGGLGINLATADTVIIYDSDWNPHNDIQAFSRAHRIGQNKKVMIYRFVTRASVEERITQVAKKKMMLTHLVVRPGLGSKTGSMSKQELDDILKFGTEELFKDEATDGGGDNKEGEDSSVIHYDDKAIERLLDRNQDETEDTELQGMNEYLSSFKVAQYVVREEEMGEEEVEREIIKQEESVDPDYWEKLLRHHYEQQQEDLARNLGKGKRIRKQVNYNDGSQEDRDWQDDQSDNQSDYSVASEEGDEDFDERSEAPRRPSRKGLRNDKDKPLPPLLARVGGNIEVLGFNARQRKAFLNAIMRYGMPPQDAFTTQWLVRDLRGKSEKEFKAYVSLFMRHLCEPGADGAETFADGVPREGLSRQHVLTRIGVMSLIRKKVQEFEHVNGRWSMPELAEVEENKKMSQPGSPSPKTPTPSTPGDTQPNTPAPVPPAEDGIKIEENSLKEEESIEGEKEVKSTAPETAIECTQAPAPASEDEKVVVEPPEGEEKVEKAEVKERTEEPMETEPKGAADVEKVEEKSAIDLTPIVVEDKEEKKEEEEKKEVMLQNGETPKDLNDEKQKKNIKQRFMFNIADGGFTELHSLWQNEERAATVTKKTYEIWHRRHDYWLLAGIINHGYARWQDIQNDPRYAILNEPFKGEMNRGNFLEIKNKFLARRFKLLEQALVIEEQLRRAAYLNMSEDPSHPSMALNTRFAEVECLAESHQHLSKESMAGNKPANAVLHKGILKQLEELLSDMKADVTRLPATIARIPPVAVRLQMSERNILSRLANRAPEPTPQQVAQQQ

Target ORF information:

RefSeq Version	XM_017018727.1
Organism	Homo sapiens(human)
Definition	Homo sapiens chromodomain helicase DNA binding protein 4 (CHD4), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017018727.1

ORF Insert Sequence:

ATGGCGTCGG GCCTGGGCTC CCCGTCCCCC TGCTCGGCGG GCAGTGAGGA GGAGGATATG 
GATGCACTTT TGAACAACAG CCTGCCCCCA CCCCACCCAG AAAATGAAGA GGACCCAGAA 
GAGGATTTGT CAGAAACAGA GACTCCAAAG CTCAAGAAGA AGAAAAAGCC TAAGAAACCT 
CGGGACCCTA AAATCCCTAA GAGCAAGCGC CAAAAAAAGG AGCGTATGCT CTTATGCCGG 
CAGCTGGGGG ACAGCTCTGG GGAGGGGCCA GAGTTTGTGG AGGAGGAGGA AGAGGTGGCT 
CTGCGCTCAG ACAGTGAGGG CAGCGACTAT ACTCCTGGCA AGAAGAAGAA GAAGAAGCTT 
GGACCTAAGA AAGAGAAGAA GAGCAAATCC AAGCGGAAGG AGGAGGAGGA GGAGGAGGAT 
GATGATGATG ATTCAAAGGA GCCTAAATCA TCTGCTCAGC TCCTGGAAGA CTGGGGCATG 
GAAGACATTG ACCACGTGTT CTCAGAGGAG GATTATCGAA CCCTCACCAA CTACAAGGCC 
TTCAGCCAGT TTGTCAGACC CCTCATTGCT GCCAAAAATC CCAAGATTGC TGTCTCCAAG 
ATGATGATGG TTTTGGGTGC AAAATGGCGG GAGTTCAGTA CCAATAACCC CTTCAAAGGC 
AGTTCTGGGG CATCAGTGGC AGCTGCGGCA GCAGCAGCGG TAGCTGTGGT GGAGAGCATG 
GTGACAGCCA CTGAGGTTGC ACCACCACCT CCCCCTGTGG AGGTGCCTAT CCGCAAGGCC 
AAGACCAAGG AGGGCAAAGG TCCCAATGCT CGGAGGAAGC CCAAGGGCAG CCCTCGTGTA 
CCTGATGCCA AGAAGCCTAA ACCCAAGAAA GTAGCTCCCC TGAAAATCAA GCTGGGAGGT 
TTTGGTTCCA AGCGTAAGAG ATCCTCGAGT GAGGATGATG ACTTAGATGT GGAATCTGAC 
TTCGATGATG CCAGTATCAA TAGCTATTCT GTTTCTGATG GTTCCACCAG CCGTAGTAGC 
CGCAGCCGCA AGAAACTCCG AACCACTAAA AAGAAAAAGA AAGGCGAGGA GGAGGTGACT 
GCTGTGGATG GTTATGAGAC AGACCACCAG GACTATTGCG AGGTGTGCCA GCAAGGCGGT 
GAGATCATCC TGTGTGATAC CTGTCCCCGT GCTTACCACA TGGTCTGCCT GGATCCCGAC 
ATGGAGAAGG CTCCCGAGGG CAAGTGGAGC TGCCCACACT GCGAGAAGGA AGGCATCCAG 
TGGGAAGCTA AAGAGGACAA TTCGGAGGGT GAGGAGATCC TGGAAGAGGT TGGGGGAGAC 
CTCGAAGAGG AGGATGACCA CCATATGGAA TTCTGTCGGG TCTGCAAGGA TGGTGGGGAA 
CTGCTCTGCT GTGATACCTG TCCTTCTTCC TACCACATCC ACTGCCTGAA TCCCCCACTT 
CCAGAGATCC CCAACGGTGA ATGGCTCTGT CCCCGTTGTA CGTGTCCAGC TCTGAAGGGC 
AAAGTGCAGA AGATCCTAAT CTGGAAGTGG GGTCAGCCAC CATCTCCCAC ACCAGTGCCT 
CGGCCTCCAG ATGCTGATCC CAACACGCCC TCCCCAAAGC CCTTGGAGGG GCGGCCAGAG 
CGGCAGTTCT TTGTGAAATG GCAAGGCATG TCTTACTGGC ACTGCTCCTG GGTTTCTGAA 
CTGCAGCTGG AGCTGCACTG TCAGGTGATG TTCCGAAACT ATCAGCGGAA GAATGATATG 
GATGAGCCAC CTTCTGGGGA CTTTGGTGGT GATGAAGAGA AAAGCCGAAA GCGAAAGAAC 
AAGGACCCTA AATTTGCAGA GATGGAGGAA CGCTTCTATC GCTATGGGAT AAAACCCGAG 
TGGATGATGA TCCACCGAAT CCTCAACCAC AGTGTGGACA AGAAGGGCCA CGTCCACTAC 
TTGATCAAGT GGCGGGACTT ACCTTACGAT CAGGCTTCTT GGGAGAGTGA GGATGTGGAG 
ATCCAGGATT ACGACCTGTT CAAGCAGAGC TATTGGAATC ACAGGGAGTT AATGAGGGGT 
GAGGAAGGCC GACCAGGCAA GAAGCTCAAG AAGGTGAAGC TTCGGAAGTT GGAGAGGCCT 
CCAGAAACGC CAACAGTTGA TCCAACAGTG AAGTATGAGC GACAGCCAGA GTACCTGGAT 
GCTACAGGTG GAACCCTGCA CCCCTATCAA ATGGAGGGCC TGAATTGGTT GCGCTTCTCC 
TGGGCTCAGG GCACTGACAC CATCTTGGCT GATGAGATGG GCCTTGGGAA AACTGTACAG 
ACAGCAGTCT TCCTGTATTC CCTTTACAAG GAGGGTCATT CCAAAGGCCC CTTCCTAGTG 
AGCGCCCCTC TTTCTACCAT CATCAACTGG GAGCGGGAGT TTGAAATGTG GGCTCCAGAC 
ATGTATGTCG TAACCTATGT GGGTGACAAG GACAGCCGTG CCATCATCCG AGAGAATGAG 
TTCTCCTTTG AAGACAATGC CATTCGTGGT GGCAAGAAGG CCTCCCGCAT GAAGAAAGAG 
GCATCTGTGA AATTCCATGT GCTGCTGACA TCCTATGAAT TGATCACCAT TGACATGGCT 
ATTTTGGGCT CTATTGATTG GGCCTGCCTC ATCGTGGATG AAGCCCATCG GCTGAAGAAC 
AATCAGTCTA AGTTCTTCCG GGTATTGAAT GGTTACTCAC TCCAGCACAA GCTGTTGCTG 
ACTGGGACAC CATTACAAAA CAATCTGGAA GAGTTGTTTC ATCTGCTCAA CTTTCTCACC 
CCCGAGAGGT TCCACAATTT GGAAGGTTTT TTGGAGGAGT TTGCTGACAT TGCCAAGGAG 
GACCAGATAA AAAAACTGCA TGACATGCTG GGGCCGCACA TGTTGCGGCG GCTCAAAGCC 
GATGTGTTCA AGAACATGCC CTCCAAGACA GAACTAATTG TGCGTGTGGA GCTGAGCCCT 
ATGCAGAAGA AATACTACAA GTACATCCTC ACTCGAAATT TTGAAGCACT CAATGCCCGA 
GGTGGTGGCA ACCAGGTGTC TCTGCTGAAT GTGGTGATGG ATCTTAAGAA GTGCTGCAAC 
CATCCATACC TCTTCCCTGT GGCTGCAATG GAAGCTCCTA AGATGCCTAA TGGCATGTAT 
GATGGCAGTG CCCTAATCAG AGCATCTGGG AAATTATTGC TGCTGCAGAA AATGCTCAAG 
AACCTTAAGG AGGGTGGGCA TCGTGTACTC ATCTTTTCCC AGATGACCAA GATGCTAGAC 
CTGCTAGAGG ATTTCTTGGA ACATGAAGGT TATAAATACG AACGCATCGA TGGTGGAATC 
ACTGGGAACA TGCGGCAAGA GGCCATTGAC CGCTTCAATG CACCGGGTGC TCAGCAGTTC 
TGCTTCTTGC TTTCCACTCG AGCTGGGGGC CTTGGAATCA ATCTGGCCAC TGCTGACACA 
GTTATTATCT ATGACTCTGA CTGGAACCCC CATAATGACA TTCAGGCCTT TAGCAGAGCT 
CACCGGATTG GGCAAAATAA AAAGGTAATG ATCTACCGGT TTGTGACCCG TGCGTCAGTG 
GAGGAGCGCA TCACGCAGGT GGCAAAGAAG AAAATGATGC TGACGCATCT AGTGGTGCGG 
CCTGGGCTGG GCTCCAAGAC TGGATCTATG TCCAAACAGG AGCTTGATGA TATCCTCAAA 
TTTGGCACTG AGGAACTATT CAAGGATGAA GCCACTGATG GAGGAGGAGA CAACAAAGAG 
GGAGAAGATA GCAGTGTTAT CCACTACGAT GATAAGGCCA TTGAACGGCT GCTAGACCGT 
AACCAGGATG AGACTGAAGA CACAGAATTG CAGGGCATGA ATGAATATTT GAGCTCATTC 
AAAGTGGCCC AGTATGTGGT ACGGGAAGAA GAAATGGGGG AAGAGGAGGT AGAACGGGAA 
ATCATTAAAC AGGAAGAAAG TGTGGATCCT GACTACTGGG AGAAATTGCT GCGGCACCAT 
TATGAGCAGC AGCAAGAAGA TCTAGCCCGA AATCTGGGCA AAGGAAAAAG AATCCGTAAA 
CAGGTCAACT ACAATGATGG CTCCCAGGAG GACCGAGATT GGCAGGACGA CCAGTCCGAC 
AACCAGTCCG ATTACTCAGT GGCTTCAGAG GAAGGTGATG AAGACTTTGA TGAACGTTCA 
GAAGCTCCCC GTAGGCCCAG TCGTAAGGGC CTGCGGAATG ATAAAGATAA GCCATTGCCT 
CCTCTGTTGG CCCGTGTTGG TGGGAATATT GAAGTACTTG GTTTTAATGC TCGTCAGCGA 
AAAGCCTTTC TTAATGCAAT TATGCGATAT GGTATGCCAC CTCAGGATGC TTTTACTACC 
CAGTGGCTTG TAAGAGACCT GCGAGGCAAA TCAGAGAAAG AGTTCAAGGC ATATGTCTCT 
CTTTTCATGC GGCATTTATG TGAGCCGGGG GCAGATGGGG CTGAGACCTT TGCTGATGGT 
GTCCCCCGAG AAGGCCTGTC TCGCCAGCAT GTCCTTACTA GAATTGGTGT TATGTCTTTG 
ATTCGCAAGA AGGTTCAGGA GTTTGAACAT GTTAATGGGC GCTGGAGCAT GCCTGAACTG 
GCTGAGGTGG AGGAAAACAA GAAGATGTCC CAGCCAGGGT CACCCTCCCC AAAAACTCCT 
ACACCCTCCA CTCCAGGGGA CACGCAGCCC AACACTCCTG CACCTGTCCC ACCTGCTGAA 
GATGGGATAA AAATAGAGGA AAATAGCCTC AAAGAAGAAG AGAGCATAGA AGGAGAAAAG 
GAGGTTAAAT CTACAGCCCC TGAGACTGCC ATTGAGTGTA CACAGGCCCC TGCCCCTGCC 
TCAGAGGATG AAAAGGTCGT TGTTGAACCC CCTGAGGGAG AGGAGAAAGT GGAAAAGGCA 
GAGGTGAAGG AGAGAACAGA GGAACCTATG GAGACAGAGC CCAAAGGTGC TGCTGATGTA 
GAGAAGGTGG AGGAAAAGTC AGCAATAGAT CTGACCCCTA TTGTGGTAGA AGACAAAGAA 
GAGAAGAAAG AAGAAGAAGA GAAAAAAGAG GTGATGCTTC AGAATGGAGA GACCCCCAAG 
GACCTGAATG ATGAGAAACA GAAGAAAAAT ATTAAACAAC GTTTCATGTT TAACATTGCA 
GATGGTGGTT TTACTGAGTT GCACTCCCTT TGGCAGAATG AAGAGCGGGC AGCCACAGTT 
ACCAAGAAGA CTTATGAGAT CTGGCATCGA CGGCATGACT ACTGGCTGCT AGCCGGCATT 
ATAAACCATG GCTATGCCCG GTGGCAAGAC ATCCAGAATG ACCCACGCTA TGCCATCCTC 
AATGAGCCTT TCAAGGGTGA AATGAACCGT GGCAATTTCT TAGAGATCAA GAATAAATTT 
CTAGCTCGAA GGTTTAAGCT CTTAGAACAA GCTCTGGTGA TTGAGGAACA GCTGCGCCGG 
GCTGCTTACT TGAACATGTC AGAAGACCCT TCTCACCCTT CCATGGCCCT CAACACCCGC 
TTTGCTGAGG TGGAGTGTTT GGCGGAAAGT CATCAGCACC TGTCCAAGGA GTCAATGGCA 
GGAAACAAGC CAGCCAATGC AGTCCTGCAC AAAGGTATTC TGAAACAGCT GGAAGAACTG 
CTGAGTGACA TGAAAGCTGA TGTGACTCGA CTCCCAGCTA CCATTGCCCG AATTCCCCCA 
GTTGCTGTGA GGTTACAGAT GTCAGAGCGT AACATTCTCA GCCGCCTGGC AAACCGGGCA 
CCCGAACCTA CCCCACAGCA GGTAGCCCAG CAGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHD4 ( XM_017018728.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016874217.1 Homo sapiens chromodomain helicase DNA binding protein 4 (CHD4), transcript variant X11, mRNA.

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CloneID	OHu83473
Clone ID Related Accession (Same CDS sequence)	XM_017018728.1
Accession Version	XM_017018728.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5736bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-25
Organism	Homo sapiens(human)
Product	chromodomain-helicase-DNA-binding protein 4 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCGG GCCTGGGCTC CCCGTCCCCC TGCTCGGCGG GCAGTGAGGA GGAGGATATG 
GATGCACTTT TGAACAACAG CCTGCCCCCA CCCCACCCAG AAAATGAAGA GGACCCAGAA 
GAGGATTTGT CAGAAACAGA GACTCCAAAG CTCAAGAAGA AGAAAAAGCC TAAGAAACCT 
CGGGACCCTA AAATCCCTAA GAGCAAGCGC CAAAAAAAGG AGCGTATGCT CTTATGCCGG 
CAGCTGGGGG ACAGCTCTGG GGAGGGGCCA GAGTTTGTGG AGGAGGAGGA AGAGGTGGCT 
CTGCGCTCAG ACAGTGAGGG CAGCGACTAT ACTCCTGGCA AGAAGAAGAA GAAGAAGCTT 
GGACCTAAGA AAGAGAAGAA GAGCAAATCC AAGCGGAAGG AGGAGGAGGA GGAGGAGGAT 
GATGATGATG ATTCAAAGGA GCCTAAATCA TCTGCTCAGC TCCTGGAAGA CTGGGGCATG 
GAAGACATTG ACCACGTGTT CTCAGAGGAG GATTATCGAA CCCTCACCAA CTACAAGGCC 
TTCAGCCAGT TTGTCAGACC CCTCATTGCT GCCAAAAATC CCAAGATTGC TGTCTCCAAG 
ATGATGATGG TTTTGGGTGC AAAATGGCGG GAGTTCAGTA CCAATAACCC CTTCAAAGGC 
AGTTCTGGGG CATCAGTGGC AGCTGCGGCA GCAGCAGCGG TAGCTGTGGT GGAGAGCATG 
GTGACAGCCA CTGAGGTTGC ACCACCACCT CCCCCTGTGG AGGTGCCTAT CCGCAAGGCC 
AAGACCAAGG AGGGCAAAGG TCCCAATGCT CGGAGGAAGC CCAAGGGCAG CCCTCGTGTA 
CCTGATGCCA AGAAGCCTAA ACCCAAGAAA GTAGCTCCCC TGAAAATCAA GCTGGGAGGT 
TTTGGTTCCA AGCGTAAGAG ATCCTCGAGT GAGGATGATG ACTTAGATGT GGAATCTGAC 
TTCGATGATG CCAGTATCAA TAGCTATTCT GTTTCTGATG GTTCCACCAG CCGTAGTAGC 
CGCAGCCGCA AGAAACTCCG AACCACTAAA AAGAAAAAGA AAGGCGAGGA GGAGGTGACT 
GCTGTGGATG GTTATGAGAC AGACCACCAG GACTATTGCG AGGTGTGCCA GCAAGGCGGT 
GAGATCATCC TGTGTGATAC CTGTCCCCGT GCTTACCACA TGGTCTGCCT GGATCCCGAC 
ATGGAGAAGG CTCCCGAGGG CAAGTGGAGC TGCCCACACT GCGAGAAGGA AGGCATCCAG 
TGGGAAGCTA AAGAGGACAA TTCGGAGGGT GAGGAGATCC TGGAAGAGGT TGGGGGAGAC 
CTCGAAGAGG AGGATGACCA CCATATGGAA TTCTGTCGGG TCTGCAAGGA TGGTGGGGAA 
CTGCTCTGCT GTGATACCTG TCCTTCTTCC TACCACATCC ACTGCCTGAA TCCCCCACTT 
CCAGAGATCC CCAACGGTGA ATGGCTCTGT CCCCGTTGTA CGTGTCCAGC TCTGAAGGGC 
AAAGTGCAGA AGATCCTAAT CTGGAAGTGG GGTCAGCCAC CATCTCCCAC ACCAGTGCCT 
CGGCCTCCAG ATGCTGATCC CAACACGCCC TCCCCAAAGC CCTTGGAGGG GCGGCCAGAG 
CGGCAGTTCT TTGTGAAATG GCAAGGCATG TCTTACTGGC ACTGCTCCTG GGTTTCTGAA 
CTGCAGCTGG AGCTGCACTG TCAGGTGATG TTCCGAAACT ATCAGCGGAA GAATGATATG 
GATGAGCCAC CTTCTGGGGA CTTTGGTGGT GATGAAGAGA AAAGCCGAAA GCGAAAGAAC 
AAGGACCCTA AATTTGCAGA GATGGAGGAA CGCTTCTATC GCTATGGGAT AAAACCCGAG 
TGGATGATGA TCCACCGAAT CCTCAACCAC AGTGTGGACA AGAAGGGCCA CGTCCACTAC 
TTGATCAAGT GGCGGGACTT ACCTTACGAT CAGGCTTCTT GGGAGAGTGA GGATGTGGAG 
ATCCAGGATT ACGACCTGTT CAAGCAGAGC TATTGGAATC ACAGGGAGTT AATGAGGGGT 
GAGGAAGGCC GACCAGGCAA GAAGCTCAAG AAGGTGAAGC TTCGGAAGTT GGAGAGGCCT 
CCAGAAACGC CAACAGTTGA TCCAACAGTG AAGTATGAGC GACAGCCAGA GTACCTGGAT 
GCTACAGGTG GAACCCTGCA CCCCTATCAA ATGGAGGGCC TGAATTGGTT GCGCTTCTCC 
TGGGCTCAGG GCACTGACAC CATCTTGGCT GATGAGATGG GCCTTGGGAA AACTGTACAG 
ACAGCAGTCT TCCTGTATTC CCTTTACAAG GAGGGTCATT CCAAAGGCCC CTTCCTAGTG 
AGCGCCCCTC TTTCTACCAT CATCAACTGG GAGCGGGAGT TTGAAATGTG GGCTCCAGAC 
ATGTATGTCG TAACCTATGT GGGTGACAAG GACAGCCGTG CCATCATCCG AGAGAATGAG 
TTCTCCTTTG AAGACAATGC CATTCGTGGT GGCAAGAAGG CCTCCCGCAT GAAGAAAGAG 
GCATCTGTGA AATTCCATGT GCTGCTGACA TCCTATGAAT TGATCACCAT TGACATGGCT 
ATTTTGGGCT CTATTGATTG GGCCTGCCTC ATCGTGGATG AAGCCCATCG GCTGAAGAAC 
AATCAGTCTA AGTTCTTCCG GGTATTGAAT GGTTACTCAC TCCAGCACAA GCTGTTGCTG 
ACTGGGACAC CATTACAAAA CAATCTGGAA GAGTTGTTTC ATCTGCTCAA CTTTCTCACC 
CCCGAGAGGT TCCACAATTT GGAAGGTTTT TTGGAGGAGT TTGCTGACAT TGCCAAGGAG 
GACCAGATAA AAAAACTGCA TGACATGCTG GGGCCGCACA TGTTGCGGCG GCTCAAAGCC 
GATGTGTTCA AGAACATGCC CTCCAAGACA GAACTAATTG TGCGTGTGGA GCTGAGCCCT 
ATGCAGAAGA AATACTACAA GTACATCCTC ACTCGAAATT TTGAAGCACT CAATGCCCGA 
GGTGGTGGCA ACCAGGTGTC TCTGCTGAAT GTGGTGATGG ATCTTAAGAA GTGCTGCAAC 
CATCCATACC TCTTCCCTGT GGCTGCAATG GAAGCTCCTA AGATGCCTAA TGGCATGTAT 
GATGGCAGTG CCCTAATCAG AGCATCTGGG AAATTATTGC TGCTGCAGAA AATGCTCAAG 
AACCTTAAGG AGGGTGGGCA TCGTGTACTC ATCTTTTCCC AGATGACCAA GATGCTAGAC 
CTGCTAGAGG ATTTCTTGGA ACATGAAGGT TATAAATACG AACGCATCGA TGGTGGAATC 
ACTGGGAACA TGCGGCAAGA GGCCATTGAC CGCTTCAATG CACCGGGTGC TCAGCAGTTC 
TGCTTCTTGC TTTCCACTCG AGCTGGGGGC CTTGGAATCA ATCTGGCCAC TGCTGACACA 
GTTATTATCT ATGACTCTGA CTGGAACCCC CATAATGACA TTCAGGCCTT TAGCAGAGCT 
CACCGGATTG GGCAAAATAA AAAGGTAATG ATCTACCGGT TTGTGACCCG TGCGTCAGTG 
GAGGAGCGCA TCACGCAGGT GGCAAAGAAG AAAATGATGC TGACGCATCT AGTGGTGCGG 
CCTGGGCTGG GCTCCAAGAC TGGATCTATG TCCAAACAGG AGCTTGATGA TATCCTCAAA 
TTTGGCACTG AGGAACTATT CAAGGATGAA GCCACTGATG GAGGAGGAGA CAACAAAGAG 
GGAGAAGATA GCAGTGTTAT CCACTACGAT GATAAGGCCA TTGAACGGCT GCTAGACCGT 
AACCAGGATG AGACTGAAGA CACAGAATTG CAGGGCATGA ATGAATATTT GAGCTCATTC 
AAAGTGGCCC AGTATGTGGT ACGGGAAGAA GAAATGGGGG AAGAGGAGGT AGAACGGGAA 
ATCATTAAAC AGGAAGAAAG TGTGGATCCT GACTACTGGG AGAAATTGCT GCGGCACCAT 
TATGAGCAGC AGCAAGAAGA TCTAGCCCGA AATCTGGGCA AAGGAAAAAG AATCCGTAAA 
CAGGTCAACT ACAATGATGG CTCCCAGGAG GACCGAGATT GGCAGGACGA CCAGTCCGAC 
AACCAGTCCG ATTACTCAGT GGCTTCAGAG GAAGGTGATG AAGACTTTGA TGAACGTTCA 
GAAGCTCCCC GTAGGCCCAG TCGTAAGGGC CTGCGGAATG ATAAAGATAA GCCATTGCCT 
CCTCTGTTGG CCCGTGTTGG TGGGAATATT GAAGTACTTG GTTTTAATGC TCGTCAGCGA 
AAAGCCTTTC TTAATGCAAT TATGCGATAT GGTATGCCAC CTCAGGATGC TTTTACTACC 
CAGTGGCTTG TAAGAGACCT GCGAGGCAAA TCAGAGAAAG AGTTCAAGGC ATATGTCTCT 
CTTTTCATGC GGCATTTATG TGAGCCGGGG GCAGATGGGG CTGAGACCTT TGCTGATGGT 
GTCCCCCGAG AAGGCCTGTC TCGCCAGCAT GTCCTTACTA GAATTGGTGT TATGTCTTTG 
ATTCGCAAGA AGGTTCAGGA GTTTGAACAT GTTAATGGGC GCTGGAGCAT GCCTGAACTG 
GCTGAGGTGG AGGAAAACAA GAAGATGTCC CAGCCAGGGT CACCCTCCCC AAAAACTCCT 
ACACCCTCCA CTCCAGGGGA CACGCAGCCC AACACTCCTG CACCTGTCCC ACCTGCTGAA 
GATGGGATAA AAATAGAGGA AAATAGCCTC AAAGAAGAAG AGAGCATAGA AGGAGAAAAG 
GAGGTTAAAT CTACAGCCCC TGAGACTGCC ATTGAGTGTA CACAGGCCCC TGCCCCTGCC 
TCAGAGGATG AAAAGGTCGT TGTTGAACCC CCTGAGGGAG AGGAGAAAGT GGAAAAGGCA 
GAGGTGAAGG AGAGAACAGA GGAACCTATG GAGACAGAGC CCAAAGGTGC TGCTGATGTA 
GAGAAGGTGG AGGAAAAGTC AGCAATAGAT CTGACCCCTA TTGTGGTAGA AGACAAAGAA 
GAGAAGAAAG AAGAAGAAGA GAAAAAAGAG GTGATGCTTC AGAATGGAGA GACCCCCAAG 
GACCTGAATG ATGAGAAACA GAAGAAAAAT ATTAAACAAC GTTTCATGTT TAACATTGCA 
GATGGTGGTT TTACTGAGTT GCACTCCCTT TGGCAGAATG AAGAGCGGGC AGCCACAGTT 
ACCAAGAAGA CTTATGAGAT CTGGCATCGA CGGCATGACT ACTGGCTGCT AGCCGGCATT 
ATAAACCATG GCTATGCCCG GTGGCAAGAC ATCCAGAATG ACCCACGCTA TGCCATCCTC 
AATGAGCCTT TCAAGGGTGA AATGAACCGT GGCAATTTCT TAGAGATCAA GAATAAATTT 
CTAGCTCGAA GGTTTAAGCT CTTAGAACAA GCTCTGGTGA TTGAGGAACA GCTGCGCCGG 
GCTGCTTACT TGAACATGTC AGAAGACCCT TCTCACCCTT CCATGGCCCT CAACACCCGC 
TTTGCTGAGG TGGAGTGTTT GGCGGAAAGT CATCAGCACC TGTCCAAGGA GTCAATGGCA 
GGAAACAAGC CAGCCAATGC AGTCCTGCAC AAAGTTCTGA AACAGCTGGA AGAACTGCTG 
AGTGACATGA AAGCTGATGT GACTCGACTC CCAGCTACCA TTGCCCGAAT TCCCCCAGTT 
GCTGTGAGGT TACAGATGTC AGAGCGTAAC ATTCTCAGCC GCCTGGCAAA CCGGGCACCC 
GAACCTACCC CACAGCAGGT AGCCCAGCAG CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016874217.1
CDS	219..5954
Translation	MASGLGSPSPCSAGSEEEDMDALLNNSLPPPHPENEEDPEEDLSETETPKLKKKKKPKKPRDPKIPKSKRQKKERMLLCRQLGDSSGEGPEFVEEEEEVALRSDSEGSDYTPGKKKKKKLGPKKEKKSKSKRKEEEEEEDDDDDSKEPKSSAQLLEDWGMEDIDHVFSEEDYRTLTNYKAFSQFVRPLIAAKNPKIAVSKMMMVLGAKWREFSTNNPFKGSSGASVAAAAAAAVAVVESMVTATEVAPPPPPVEVPIRKAKTKEGKGPNARRKPKGSPRVPDAKKPKPKKVAPLKIKLGGFGSKRKRSSSEDDDLDVESDFDDASINSYSVSDGSTSRSSRSRKKLRTTKKKKKGEEEVTAVDGYETDHQDYCEVCQQGGEIILCDTCPRAYHMVCLDPDMEKAPEGKWSCPHCEKEGIQWEAKEDNSEGEEILEEVGGDLEEEDDHHMEFCRVCKDGGELLCCDTCPSSYHIHCLNPPLPEIPNGEWLCPRCTCPALKGKVQKILIWKWGQPPSPTPVPRPPDADPNTPSPKPLEGRPERQFFVKWQGMSYWHCSWVSELQLELHCQVMFRNYQRKNDMDEPPSGDFGGDEEKSRKRKNKDPKFAEMEERFYRYGIKPEWMMIHRILNHSVDKKGHVHYLIKWRDLPYDQASWESEDVEIQDYDLFKQSYWNHRELMRGEEGRPGKKLKKVKLRKLERPPETPTVDPTVKYERQPEYLDATGGTLHPYQMEGLNWLRFSWAQGTDTILADEMGLGKTVQTAVFLYSLYKEGHSKGPFLVSAPLSTIINWEREFEMWAPDMYVVTYVGDKDSRAIIRENEFSFEDNAIRGGKKASRMKKEASVKFHVLLTSYELITIDMAILGSIDWACLIVDEAHRLKNNQSKFFRVLNGYSLQHKLLLTGTPLQNNLEELFHLLNFLTPERFHNLEGFLEEFADIAKEDQIKKLHDMLGPHMLRRLKADVFKNMPSKTELIVRVELSPMQKKYYKYILTRNFEALNARGGGNQVSLLNVVMDLKKCCNHPYLFPVAAMEAPKMPNGMYDGSALIRASGKLLLLQKMLKNLKEGGHRVLIFSQMTKMLDLLEDFLEHEGYKYERIDGGITGNMRQEAIDRFNAPGAQQFCFLLSTRAGGLGINLATADTVIIYDSDWNPHNDIQAFSRAHRIGQNKKVMIYRFVTRASVEERITQVAKKKMMLTHLVVRPGLGSKTGSMSKQELDDILKFGTEELFKDEATDGGGDNKEGEDSSVIHYDDKAIERLLDRNQDETEDTELQGMNEYLSSFKVAQYVVREEEMGEEEVEREIIKQEESVDPDYWEKLLRHHYEQQQEDLARNLGKGKRIRKQVNYNDGSQEDRDWQDDQSDNQSDYSVASEEGDEDFDERSEAPRRPSRKGLRNDKDKPLPPLLARVGGNIEVLGFNARQRKAFLNAIMRYGMPPQDAFTTQWLVRDLRGKSEKEFKAYVSLFMRHLCEPGADGAETFADGVPREGLSRQHVLTRIGVMSLIRKKVQEFEHVNGRWSMPELAEVEENKKMSQPGSPSPKTPTPSTPGDTQPNTPAPVPPAEDGIKIEENSLKEEESIEGEKEVKSTAPETAIECTQAPAPASEDEKVVVEPPEGEEKVEKAEVKERTEEPMETEPKGAADVEKVEEKSAIDLTPIVVEDKEEKKEEEEKKEVMLQNGETPKDLNDEKQKKNIKQRFMFNIADGGFTELHSLWQNEERAATVTKKTYEIWHRRHDYWLLAGIINHGYARWQDIQNDPRYAILNEPFKGEMNRGNFLEIKNKFLARRFKLLEQALVIEEQLRRAAYLNMSEDPSHPSMALNTRFAEVECLAESHQHLSKESMAGNKPANAVLHKVLKQLEELLSDMKADVTRLPATIARIPPVAVRLQMSERNILSRLANRAPEPTPQQVAQQQ

Target ORF information:

RefSeq Version	XM_017018728.1
Organism	Homo sapiens(human)
Definition	Homo sapiens chromodomain helicase DNA binding protein 4 (CHD4), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017018728.1

ORF Insert Sequence:

ATGGCGTCGG GCCTGGGCTC CCCGTCCCCC TGCTCGGCGG GCAGTGAGGA GGAGGATATG 
GATGCACTTT TGAACAACAG CCTGCCCCCA CCCCACCCAG AAAATGAAGA GGACCCAGAA 
GAGGATTTGT CAGAAACAGA GACTCCAAAG CTCAAGAAGA AGAAAAAGCC TAAGAAACCT 
CGGGACCCTA AAATCCCTAA GAGCAAGCGC CAAAAAAAGG AGCGTATGCT CTTATGCCGG 
CAGCTGGGGG ACAGCTCTGG GGAGGGGCCA GAGTTTGTGG AGGAGGAGGA AGAGGTGGCT 
CTGCGCTCAG ACAGTGAGGG CAGCGACTAT ACTCCTGGCA AGAAGAAGAA GAAGAAGCTT 
GGACCTAAGA AAGAGAAGAA GAGCAAATCC AAGCGGAAGG AGGAGGAGGA GGAGGAGGAT 
GATGATGATG ATTCAAAGGA GCCTAAATCA TCTGCTCAGC TCCTGGAAGA CTGGGGCATG 
GAAGACATTG ACCACGTGTT CTCAGAGGAG GATTATCGAA CCCTCACCAA CTACAAGGCC 
TTCAGCCAGT TTGTCAGACC CCTCATTGCT GCCAAAAATC CCAAGATTGC TGTCTCCAAG 
ATGATGATGG TTTTGGGTGC AAAATGGCGG GAGTTCAGTA CCAATAACCC CTTCAAAGGC 
AGTTCTGGGG CATCAGTGGC AGCTGCGGCA GCAGCAGCGG TAGCTGTGGT GGAGAGCATG 
GTGACAGCCA CTGAGGTTGC ACCACCACCT CCCCCTGTGG AGGTGCCTAT CCGCAAGGCC 
AAGACCAAGG AGGGCAAAGG TCCCAATGCT CGGAGGAAGC CCAAGGGCAG CCCTCGTGTA 
CCTGATGCCA AGAAGCCTAA ACCCAAGAAA GTAGCTCCCC TGAAAATCAA GCTGGGAGGT 
TTTGGTTCCA AGCGTAAGAG ATCCTCGAGT GAGGATGATG ACTTAGATGT GGAATCTGAC 
TTCGATGATG CCAGTATCAA TAGCTATTCT GTTTCTGATG GTTCCACCAG CCGTAGTAGC 
CGCAGCCGCA AGAAACTCCG AACCACTAAA AAGAAAAAGA AAGGCGAGGA GGAGGTGACT 
GCTGTGGATG GTTATGAGAC AGACCACCAG GACTATTGCG AGGTGTGCCA GCAAGGCGGT 
GAGATCATCC TGTGTGATAC CTGTCCCCGT GCTTACCACA TGGTCTGCCT GGATCCCGAC 
ATGGAGAAGG CTCCCGAGGG CAAGTGGAGC TGCCCACACT GCGAGAAGGA AGGCATCCAG 
TGGGAAGCTA AAGAGGACAA TTCGGAGGGT GAGGAGATCC TGGAAGAGGT TGGGGGAGAC 
CTCGAAGAGG AGGATGACCA CCATATGGAA TTCTGTCGGG TCTGCAAGGA TGGTGGGGAA 
CTGCTCTGCT GTGATACCTG TCCTTCTTCC TACCACATCC ACTGCCTGAA TCCCCCACTT 
CCAGAGATCC CCAACGGTGA ATGGCTCTGT CCCCGTTGTA CGTGTCCAGC TCTGAAGGGC 
AAAGTGCAGA AGATCCTAAT CTGGAAGTGG GGTCAGCCAC CATCTCCCAC ACCAGTGCCT 
CGGCCTCCAG ATGCTGATCC CAACACGCCC TCCCCAAAGC CCTTGGAGGG GCGGCCAGAG 
CGGCAGTTCT TTGTGAAATG GCAAGGCATG TCTTACTGGC ACTGCTCCTG GGTTTCTGAA 
CTGCAGCTGG AGCTGCACTG TCAGGTGATG TTCCGAAACT ATCAGCGGAA GAATGATATG 
GATGAGCCAC CTTCTGGGGA CTTTGGTGGT GATGAAGAGA AAAGCCGAAA GCGAAAGAAC 
AAGGACCCTA AATTTGCAGA GATGGAGGAA CGCTTCTATC GCTATGGGAT AAAACCCGAG 
TGGATGATGA TCCACCGAAT CCTCAACCAC AGTGTGGACA AGAAGGGCCA CGTCCACTAC 
TTGATCAAGT GGCGGGACTT ACCTTACGAT CAGGCTTCTT GGGAGAGTGA GGATGTGGAG 
ATCCAGGATT ACGACCTGTT CAAGCAGAGC TATTGGAATC ACAGGGAGTT AATGAGGGGT 
GAGGAAGGCC GACCAGGCAA GAAGCTCAAG AAGGTGAAGC TTCGGAAGTT GGAGAGGCCT 
CCAGAAACGC CAACAGTTGA TCCAACAGTG AAGTATGAGC GACAGCCAGA GTACCTGGAT 
GCTACAGGTG GAACCCTGCA CCCCTATCAA ATGGAGGGCC TGAATTGGTT GCGCTTCTCC 
TGGGCTCAGG GCACTGACAC CATCTTGGCT GATGAGATGG GCCTTGGGAA AACTGTACAG 
ACAGCAGTCT TCCTGTATTC CCTTTACAAG GAGGGTCATT CCAAAGGCCC CTTCCTAGTG 
AGCGCCCCTC TTTCTACCAT CATCAACTGG GAGCGGGAGT TTGAAATGTG GGCTCCAGAC 
ATGTATGTCG TAACCTATGT GGGTGACAAG GACAGCCGTG CCATCATCCG AGAGAATGAG 
TTCTCCTTTG AAGACAATGC CATTCGTGGT GGCAAGAAGG CCTCCCGCAT GAAGAAAGAG 
GCATCTGTGA AATTCCATGT GCTGCTGACA TCCTATGAAT TGATCACCAT TGACATGGCT 
ATTTTGGGCT CTATTGATTG GGCCTGCCTC ATCGTGGATG AAGCCCATCG GCTGAAGAAC 
AATCAGTCTA AGTTCTTCCG GGTATTGAAT GGTTACTCAC TCCAGCACAA GCTGTTGCTG 
ACTGGGACAC CATTACAAAA CAATCTGGAA GAGTTGTTTC ATCTGCTCAA CTTTCTCACC 
CCCGAGAGGT TCCACAATTT GGAAGGTTTT TTGGAGGAGT TTGCTGACAT TGCCAAGGAG 
GACCAGATAA AAAAACTGCA TGACATGCTG GGGCCGCACA TGTTGCGGCG GCTCAAAGCC 
GATGTGTTCA AGAACATGCC CTCCAAGACA GAACTAATTG TGCGTGTGGA GCTGAGCCCT 
ATGCAGAAGA AATACTACAA GTACATCCTC ACTCGAAATT TTGAAGCACT CAATGCCCGA 
GGTGGTGGCA ACCAGGTGTC TCTGCTGAAT GTGGTGATGG ATCTTAAGAA GTGCTGCAAC 
CATCCATACC TCTTCCCTGT GGCTGCAATG GAAGCTCCTA AGATGCCTAA TGGCATGTAT 
GATGGCAGTG CCCTAATCAG AGCATCTGGG AAATTATTGC TGCTGCAGAA AATGCTCAAG 
AACCTTAAGG AGGGTGGGCA TCGTGTACTC ATCTTTTCCC AGATGACCAA GATGCTAGAC 
CTGCTAGAGG ATTTCTTGGA ACATGAAGGT TATAAATACG AACGCATCGA TGGTGGAATC 
ACTGGGAACA TGCGGCAAGA GGCCATTGAC CGCTTCAATG CACCGGGTGC TCAGCAGTTC 
TGCTTCTTGC TTTCCACTCG AGCTGGGGGC CTTGGAATCA ATCTGGCCAC TGCTGACACA 
GTTATTATCT ATGACTCTGA CTGGAACCCC CATAATGACA TTCAGGCCTT TAGCAGAGCT 
CACCGGATTG GGCAAAATAA AAAGGTAATG ATCTACCGGT TTGTGACCCG TGCGTCAGTG 
GAGGAGCGCA TCACGCAGGT GGCAAAGAAG AAAATGATGC TGACGCATCT AGTGGTGCGG 
CCTGGGCTGG GCTCCAAGAC TGGATCTATG TCCAAACAGG AGCTTGATGA TATCCTCAAA 
TTTGGCACTG AGGAACTATT CAAGGATGAA GCCACTGATG GAGGAGGAGA CAACAAAGAG 
GGAGAAGATA GCAGTGTTAT CCACTACGAT GATAAGGCCA TTGAACGGCT GCTAGACCGT 
AACCAGGATG AGACTGAAGA CACAGAATTG CAGGGCATGA ATGAATATTT GAGCTCATTC 
AAAGTGGCCC AGTATGTGGT ACGGGAAGAA GAAATGGGGG AAGAGGAGGT AGAACGGGAA 
ATCATTAAAC AGGAAGAAAG TGTGGATCCT GACTACTGGG AGAAATTGCT GCGGCACCAT 
TATGAGCAGC AGCAAGAAGA TCTAGCCCGA AATCTGGGCA AAGGAAAAAG AATCCGTAAA 
CAGGTCAACT ACAATGATGG CTCCCAGGAG GACCGAGATT GGCAGGACGA CCAGTCCGAC 
AACCAGTCCG ATTACTCAGT GGCTTCAGAG GAAGGTGATG AAGACTTTGA TGAACGTTCA 
GAAGCTCCCC GTAGGCCCAG TCGTAAGGGC CTGCGGAATG ATAAAGATAA GCCATTGCCT 
CCTCTGTTGG CCCGTGTTGG TGGGAATATT GAAGTACTTG GTTTTAATGC TCGTCAGCGA 
AAAGCCTTTC TTAATGCAAT TATGCGATAT GGTATGCCAC CTCAGGATGC TTTTACTACC 
CAGTGGCTTG TAAGAGACCT GCGAGGCAAA TCAGAGAAAG AGTTCAAGGC ATATGTCTCT 
CTTTTCATGC GGCATTTATG TGAGCCGGGG GCAGATGGGG CTGAGACCTT TGCTGATGGT 
GTCCCCCGAG AAGGCCTGTC TCGCCAGCAT GTCCTTACTA GAATTGGTGT TATGTCTTTG 
ATTCGCAAGA AGGTTCAGGA GTTTGAACAT GTTAATGGGC GCTGGAGCAT GCCTGAACTG 
GCTGAGGTGG AGGAAAACAA GAAGATGTCC CAGCCAGGGT CACCCTCCCC AAAAACTCCT 
ACACCCTCCA CTCCAGGGGA CACGCAGCCC AACACTCCTG CACCTGTCCC ACCTGCTGAA 
GATGGGATAA AAATAGAGGA AAATAGCCTC AAAGAAGAAG AGAGCATAGA AGGAGAAAAG 
GAGGTTAAAT CTACAGCCCC TGAGACTGCC ATTGAGTGTA CACAGGCCCC TGCCCCTGCC 
TCAGAGGATG AAAAGGTCGT TGTTGAACCC CCTGAGGGAG AGGAGAAAGT GGAAAAGGCA 
GAGGTGAAGG AGAGAACAGA GGAACCTATG GAGACAGAGC CCAAAGGTGC TGCTGATGTA 
GAGAAGGTGG AGGAAAAGTC AGCAATAGAT CTGACCCCTA TTGTGGTAGA AGACAAAGAA 
GAGAAGAAAG AAGAAGAAGA GAAAAAAGAG GTGATGCTTC AGAATGGAGA GACCCCCAAG 
GACCTGAATG ATGAGAAACA GAAGAAAAAT ATTAAACAAC GTTTCATGTT TAACATTGCA 
GATGGTGGTT TTACTGAGTT GCACTCCCTT TGGCAGAATG AAGAGCGGGC AGCCACAGTT 
ACCAAGAAGA CTTATGAGAT CTGGCATCGA CGGCATGACT ACTGGCTGCT AGCCGGCATT 
ATAAACCATG GCTATGCCCG GTGGCAAGAC ATCCAGAATG ACCCACGCTA TGCCATCCTC 
AATGAGCCTT TCAAGGGTGA AATGAACCGT GGCAATTTCT TAGAGATCAA GAATAAATTT 
CTAGCTCGAA GGTTTAAGCT CTTAGAACAA GCTCTGGTGA TTGAGGAACA GCTGCGCCGG 
GCTGCTTACT TGAACATGTC AGAAGACCCT TCTCACCCTT CCATGGCCCT CAACACCCGC 
TTTGCTGAGG TGGAGTGTTT GGCGGAAAGT CATCAGCACC TGTCCAAGGA GTCAATGGCA 
GGAAACAAGC CAGCCAATGC AGTCCTGCAC AAAGTTCTGA AACAGCTGGA AGAACTGCTG 
AGTGACATGA AAGCTGATGT GACTCGACTC CCAGCTACCA TTGCCCGAAT TCCCCCAGTT 
GCTGTGAGGT TACAGATGTC AGAGCGTAAC ATTCTCAGCC GCCTGGCAAA CCGGGCACCC 
GAACCTACCC CACAGCAGGT AGCCCAGCAG CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHD4 ( XM_017018729.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016874218.1 Homo sapiens chromodomain helicase DNA binding protein 4 (CHD4), transcript variant X13, mRNA.

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CloneID	OHu83474
Clone ID Related Accession (Same CDS sequence)	XM_017018729.1
Accession Version	XM_017018729.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5721bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-25
Organism	Homo sapiens(human)
Product	chromodomain-helicase-DNA-binding protein 4 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCGG GCCTGGGCTC CCCGTCCCCC TGCTCGGCGG GCAGTGAGGA GGAGGATATG 
GATGCACTTT TGAACAACAG CCTGCCCCCA CCCCACCCAG AAAATGAAGA GGACCCAGAA 
GAGGATTTGT CAGAAACAGA GACTCCAAAG CTCAAGAAGA AGAAAAAGCC TAAGAAACCT 
CGGGACCCTA AAATCCCTAA GAGCAAGCGC CAAAAAAAGG AGCTGGGGGA CAGCTCTGGG 
GAGGGGCCAG AGTTTGTGGA GGAGGAGGAA GAGGTGGCTC TGCGCTCAGA CAGTGAGGGC 
AGCGACTATA CTCCTGGCAA GAAGAAGAAG AAGAAGCTTG GACCTAAGAA AGAGAAGAAG 
AGCAAATCCA AGCGGAAGGA GGAGGAGGAG GAGGAGGATG ATGATGATGA TTCAAAGGAG 
CCTAAATCAT CTGCTCAGCT CCTGGAAGAC TGGGGCATGG AAGACATTGA CCACGTGTTC 
TCAGAGGAGG ATTATCGAAC CCTCACCAAC TACAAGGCCT TCAGCCAGTT TGTCAGACCC 
CTCATTGCTG CCAAAAATCC CAAGATTGCT GTCTCCAAGA TGATGATGGT TTTGGGTGCA 
AAATGGCGGG AGTTCAGTAC CAATAACCCC TTCAAAGGCA GTTCTGGGGC ATCAGTGGCA 
GCTGCGGCAG CAGCAGCGGT AGCTGTGGTG GAGAGCATGG TGACAGCCAC TGAGGTTGCA 
CCACCACCTC CCCCTGTGGA GGTGCCTATC CGCAAGGCCA AGACCAAGGA GGGCAAAGGT 
CCCAATGCTC GGAGGAAGCC CAAGGGCAGC CCTCGTGTAC CTGATGCCAA GAAGCCTAAA 
CCCAAGAAAG TAGCTCCCCT GAAAATCAAG CTGGGAGGTT TTGGTTCCAA GCGTAAGAGA 
TCCTCGAGTG AGGATGATGA CTTAGATGTG GAATCTGACT TCGATGATGC CAGTATCAAT 
AGCTATTCTG TTTCTGATGG TTCCACCAGC CGTAGTAGCC GCAGCCGCAA GAAACTCCGA 
ACCACTAAAA AGAAAAAGAA AGGCGAGGAG GAGGTGACTG CTGTGGATGG TTATGAGACA 
GACCACCAGG ACTATTGCGA GGTGTGCCAG CAAGGCGGTG AGATCATCCT GTGTGATACC 
TGTCCCCGTG CTTACCACAT GGTCTGCCTG GATCCCGACA TGGAGAAGGC TCCCGAGGGC 
AAGTGGAGCT GCCCACACTG CGAGAAGGAA GGCATCCAGT GGGAAGCTAA AGAGGACAAT 
TCGGAGGGTG AGGAGATCCT GGAAGAGGTT GGGGGAGACC TCGAAGAGGA GGATGACCAC 
CATATGGAAT TCTGTCGGGT CTGCAAGGAT GGTGGGGAAC TGCTCTGCTG TGATACCTGT 
CCTTCTTCCT ACCACATCCA CTGCCTGAAT CCCCCACTTC CAGAGATCCC CAACGGTGAA 
TGGCTCTGTC CCCGTTGTAC GTGTCCAGCT CTGAAGGGCA AAGTGCAGAA GATCCTAATC 
TGGAAGTGGG GTCAGCCACC ATCTCCCACA CCAGTGCCTC GGCCTCCAGA TGCTGATCCC 
AACACGCCCT CCCCAAAGCC CTTGGAGGGG CGGCCAGAGC GGCAGTTCTT TGTGAAATGG 
CAAGGCATGT CTTACTGGCA CTGCTCCTGG GTTTCTGAAC TGCAGCTGGA GCTGCACTGT 
CAGGTGATGT TCCGAAACTA TCAGCGGAAG AATGATATGG ATGAGCCACC TTCTGGGGAC 
TTTGGTGGTG ATGAAGAGAA AAGCCGAAAG CGAAAGAACA AGGACCCTAA ATTTGCAGAG 
ATGGAGGAAC GCTTCTATCG CTATGGGATA AAACCCGAGT GGATGATGAT CCACCGAATC 
CTCAACCACA GTGTGGACAA GAAGGGCCAC GTCCACTACT TGATCAAGTG GCGGGACTTA 
CCTTACGATC AGGCTTCTTG GGAGAGTGAG GATGTGGAGA TCCAGGATTA CGACCTGTTC 
AAGCAGAGCT ATTGGAATCA CAGGGAGTTA ATGAGGGGTG AGGAAGGCCG ACCAGGCAAG 
AAGCTCAAGA AGGTGAAGCT TCGGAAGTTG GAGAGGCCTC CAGAAACGCC AACAGTTGAT 
CCAACAGTGA AGTATGAGCG ACAGCCAGAG TACCTGGATG CTACAGGTGG AACCCTGCAC 
CCCTATCAAA TGGAGGGCCT GAATTGGTTG CGCTTCTCCT GGGCTCAGGG CACTGACACC 
ATCTTGGCTG ATGAGATGGG CCTTGGGAAA ACTGTACAGA CAGCAGTCTT CCTGTATTCC 
CTTTACAAGG AGGGTCATTC CAAAGGCCCC TTCCTAGTGA GCGCCCCTCT TTCTACCATC 
ATCAACTGGG AGCGGGAGTT TGAAATGTGG GCTCCAGACA TGTATGTCGT AACCTATGTG 
GGTGACAAGG ACAGCCGTGC CATCATCCGA GAGAATGAGT TCTCCTTTGA AGACAATGCC 
ATTCGTGGTG GCAAGAAGGC CTCCCGCATG AAGAAAGAGG CATCTGTGAA ATTCCATGTG 
CTGCTGACAT CCTATGAATT GATCACCATT GACATGGCTA TTTTGGGCTC TATTGATTGG 
GCCTGCCTCA TCGTGGATGA AGCCCATCGG CTGAAGAACA ATCAGTCTAA GTTCTTCCGG 
GTATTGAATG GTTACTCACT CCAGCACAAG CTGTTGCTGA CTGGGACACC ATTACAAAAC 
AATCTGGAAG AGTTGTTTCA TCTGCTCAAC TTTCTCACCC CCGAGAGGTT CCACAATTTG 
GAAGGTTTTT TGGAGGAGTT TGCTGACATT GCCAAGGAGG ACCAGATAAA AAAACTGCAT 
GACATGCTGG GGCCGCACAT GTTGCGGCGG CTCAAAGCCG ATGTGTTCAA GAACATGCCC 
TCCAAGACAG AACTAATTGT GCGTGTGGAG CTGAGCCCTA TGCAGAAGAA ATACTACAAG 
TACATCCTCA CTCGAAATTT TGAAGCACTC AATGCCCGAG GTGGTGGCAA CCAGGTGTCT 
CTGCTGAATG TGGTGATGGA TCTTAAGAAG TGCTGCAACC ATCCATACCT CTTCCCTGTG 
GCTGCAATGG AAGCTCCTAA GATGCCTAAT GGCATGTATG ATGGCAGTGC CCTAATCAGA 
GCATCTGGGA AATTATTGCT GCTGCAGAAA ATGCTCAAGA ACCTTAAGGA GGGTGGGCAT 
CGTGTACTCA TCTTTTCCCA GATGACCAAG ATGCTAGACC TGCTAGAGGA TTTCTTGGAA 
CATGAAGGTT ATAAATACGA ACGCATCGAT GGTGGAATCA CTGGGAACAT GCGGCAAGAG 
GCCATTGACC GCTTCAATGC ACCGGGTGCT CAGCAGTTCT GCTTCTTGCT TTCCACTCGA 
GCTGGGGGCC TTGGAATCAA TCTGGCCACT GCTGACACAG TTATTATCTA TGACTCTGAC 
TGGAACCCCC ATAATGACAT TCAGGCCTTT AGCAGAGCTC ACCGGATTGG GCAAAATAAA 
AAGGTAATGA TCTACCGGTT TGTGACCCGT GCGTCAGTGG AGGAGCGCAT CACGCAGGTG 
GCAAAGAAGA AAATGATGCT GACGCATCTA GTGGTGCGGC CTGGGCTGGG CTCCAAGACT 
GGATCTATGT CCAAACAGGA GCTTGATGAT ATCCTCAAAT TTGGCACTGA GGAACTATTC 
AAGGATGAAG CCACTGATGG AGGAGGAGAC AACAAAGAGG GAGAAGATAG CAGTGTTATC 
CACTACGATG ATAAGGCCAT TGAACGGCTG CTAGACCGTA ACCAGGATGA GACTGAAGAC 
ACAGAATTGC AGGGCATGAA TGAATATTTG AGCTCATTCA AAGTGGCCCA GTATGTGGTA 
CGGGAAGAAG AAATGGGGGA GGAAGAGGAG GTAGAACGGG AAATCATTAA ACAGGAAGAA 
AGTGTGGATC CTGACTACTG GGAGAAATTG CTGCGGCACC ATTATGAGCA GCAGCAAGAA 
GATCTAGCCC GAAATCTGGG CAAAGGAAAA AGAATCCGTA AACAGGTCAA CTACAATGAT 
GGCTCCCAGG AGGACCGAGA TTGGCAGGAC GACCAGTCCG ACAACCAGTC CGATTACTCA 
GTGGCTTCAG AGGAAGGTGA TGAAGACTTT GATGAACGTT CAGAAGCTCC CCGTAGGCCC 
AGTCGTAAGG GCCTGCGGAA TGATAAAGAT AAGCCATTGC CTCCTCTGTT GGCCCGTGTT 
GGTGGGAATA TTGAAGTACT TGGTTTTAAT GCTCGTCAGC GAAAAGCCTT TCTTAATGCA 
ATTATGCGAT ATGGTATGCC ACCTCAGGAT GCTTTTACTA CCCAGTGGCT TGTAAGAGAC 
CTGCGAGGCA AATCAGAGAA AGAGTTCAAG GCATATGTCT CTCTTTTCAT GCGGCATTTA 
TGTGAGCCGG GGGCAGATGG GGCTGAGACC TTTGCTGATG GTGTCCCCCG AGAAGGCCTG 
TCTCGCCAGC ATGTCCTTAC TAGAATTGGT GTTATGTCTT TGATTCGCAA GAAGGTTCAG 
GAGTTTGAAC ATGTTAATGG GCGCTGGAGC ATGCCTGAAC TGGCTGAGGT GGAGGAAAAC 
AAGAAGATGT CCCAGCCAGG GTCACCCTCC CCAAAAACTC CTACACCCTC CACTCCAGGG 
GACACGCAGC CCAACACTCC TGCACCTGTC CCACCTGCTG AAGATGGGAT AAAAATAGAG 
GAAAATAGCC TCAAAGAAGA AGAGAGCATA GAAGGAGAAA AGGAGGTTAA ATCTACAGCC 
CCTGAGACTG CCATTGAGTG TACACAGGCC CCTGCCCCTG CCTCAGAGGA TGAAAAGGTC 
GTTGTTGAAC CCCCTGAGGG AGAGGAGAAA GTGGAAAAGG CAGAGGTGAA GGAGAGAACA 
GAGGAACCTA TGGAGACAGA GCCCAAAGGT GCTGCTGATG TAGAGAAGGT GGAGGAAAAG 
TCAGCAATAG ATCTGACCCC TATTGTGGTA GAAGACAAAG AAGAGAAGAA AGAAGAAGAA 
GAGAAAAAAG AGGTGATGCT TCAGAATGGA GAGACCCCCA AGGACCTGAA TGATGAGAAA 
CAGAAGAAAA ATATTAAACA ACGTTTCATG TTTAACATTG CAGATGGTGG TTTTACTGAG 
TTGCACTCCC TTTGGCAGAA TGAAGAGCGG GCAGCCACAG TTACCAAGAA GACTTATGAG 
ATCTGGCATC GACGGCATGA CTACTGGCTG CTAGCCGGCA TTATAAACCA TGGCTATGCC 
CGGTGGCAAG ACATCCAGAA TGACCCACGC TATGCCATCC TCAATGAGCC TTTCAAGGGT 
GAAATGAACC GTGGCAATTT CTTAGAGATC AAGAATAAAT TTCTAGCTCG AAGGTTTAAG 
CTCTTAGAAC AAGCTCTGGT GATTGAGGAA CAGCTGCGCC GGGCTGCTTA CTTGAACATG 
TCAGAAGACC CTTCTCACCC TTCCATGGCC CTCAACACCC GCTTTGCTGA GGTGGAGTGT 
TTGGCGGAAA GTCATCAGCA CCTGTCCAAG GAGTCAATGG CAGGAAACAA GCCAGCCAAT 
GCAGTCCTGC ACAAAGGTAT TCTGAAACAG CTGGAAGAAC TGCTGAGTGA CATGAAAGCT 
GATGTGACTC GACTCCCAGC TACCATTGCC CGAATTCCCC CAGTTGCTGT GAGGTTACAG 
ATGTCAGAGC GTAACATTCT CAGCCGCCTG GCAAACCGGG CACCCGAACC TACCCCACAG 
CAGGTAGCCC AGCAGCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016874218.1
CDS	165..5885
Translation	MASGLGSPSPCSAGSEEEDMDALLNNSLPPPHPENEEDPEEDLSETETPKLKKKKKPKKPRDPKIPKSKRQKKELGDSSGEGPEFVEEEEEVALRSDSEGSDYTPGKKKKKKLGPKKEKKSKSKRKEEEEEEDDDDDSKEPKSSAQLLEDWGMEDIDHVFSEEDYRTLTNYKAFSQFVRPLIAAKNPKIAVSKMMMVLGAKWREFSTNNPFKGSSGASVAAAAAAAVAVVESMVTATEVAPPPPPVEVPIRKAKTKEGKGPNARRKPKGSPRVPDAKKPKPKKVAPLKIKLGGFGSKRKRSSSEDDDLDVESDFDDASINSYSVSDGSTSRSSRSRKKLRTTKKKKKGEEEVTAVDGYETDHQDYCEVCQQGGEIILCDTCPRAYHMVCLDPDMEKAPEGKWSCPHCEKEGIQWEAKEDNSEGEEILEEVGGDLEEEDDHHMEFCRVCKDGGELLCCDTCPSSYHIHCLNPPLPEIPNGEWLCPRCTCPALKGKVQKILIWKWGQPPSPTPVPRPPDADPNTPSPKPLEGRPERQFFVKWQGMSYWHCSWVSELQLELHCQVMFRNYQRKNDMDEPPSGDFGGDEEKSRKRKNKDPKFAEMEERFYRYGIKPEWMMIHRILNHSVDKKGHVHYLIKWRDLPYDQASWESEDVEIQDYDLFKQSYWNHRELMRGEEGRPGKKLKKVKLRKLERPPETPTVDPTVKYERQPEYLDATGGTLHPYQMEGLNWLRFSWAQGTDTILADEMGLGKTVQTAVFLYSLYKEGHSKGPFLVSAPLSTIINWEREFEMWAPDMYVVTYVGDKDSRAIIRENEFSFEDNAIRGGKKASRMKKEASVKFHVLLTSYELITIDMAILGSIDWACLIVDEAHRLKNNQSKFFRVLNGYSLQHKLLLTGTPLQNNLEELFHLLNFLTPERFHNLEGFLEEFADIAKEDQIKKLHDMLGPHMLRRLKADVFKNMPSKTELIVRVELSPMQKKYYKYILTRNFEALNARGGGNQVSLLNVVMDLKKCCNHPYLFPVAAMEAPKMPNGMYDGSALIRASGKLLLLQKMLKNLKEGGHRVLIFSQMTKMLDLLEDFLEHEGYKYERIDGGITGNMRQEAIDRFNAPGAQQFCFLLSTRAGGLGINLATADTVIIYDSDWNPHNDIQAFSRAHRIGQNKKVMIYRFVTRASVEERITQVAKKKMMLTHLVVRPGLGSKTGSMSKQELDDILKFGTEELFKDEATDGGGDNKEGEDSSVIHYDDKAIERLLDRNQDETEDTELQGMNEYLSSFKVAQYVVREEEMGEEEEVEREIIKQEESVDPDYWEKLLRHHYEQQQEDLARNLGKGKRIRKQVNYNDGSQEDRDWQDDQSDNQSDYSVASEEGDEDFDERSEAPRRPSRKGLRNDKDKPLPPLLARVGGNIEVLGFNARQRKAFLNAIMRYGMPPQDAFTTQWLVRDLRGKSEKEFKAYVSLFMRHLCEPGADGAETFADGVPREGLSRQHVLTRIGVMSLIRKKVQEFEHVNGRWSMPELAEVEENKKMSQPGSPSPKTPTPSTPGDTQPNTPAPVPPAEDGIKIEENSLKEEESIEGEKEVKSTAPETAIECTQAPAPASEDEKVVVEPPEGEEKVEKAEVKERTEEPMETEPKGAADVEKVEEKSAIDLTPIVVEDKEEKKEEEEKKEVMLQNGETPKDLNDEKQKKNIKQRFMFNIADGGFTELHSLWQNEERAATVTKKTYEIWHRRHDYWLLAGIINHGYARWQDIQNDPRYAILNEPFKGEMNRGNFLEIKNKFLARRFKLLEQALVIEEQLRRAAYLNMSEDPSHPSMALNTRFAEVECLAESHQHLSKESMAGNKPANAVLHKGILKQLEELLSDMKADVTRLPATIARIPPVAVRLQMSERNILSRLANRAPEPTPQQVAQQQ

Target ORF information:

RefSeq Version	XM_017018729.1
Organism	Homo sapiens(human)
Definition	Homo sapiens chromodomain helicase DNA binding protein 4 (CHD4), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017018729.1

ORF Insert Sequence:

ATGGCGTCGG GCCTGGGCTC CCCGTCCCCC TGCTCGGCGG GCAGTGAGGA GGAGGATATG 
GATGCACTTT TGAACAACAG CCTGCCCCCA CCCCACCCAG AAAATGAAGA GGACCCAGAA 
GAGGATTTGT CAGAAACAGA GACTCCAAAG CTCAAGAAGA AGAAAAAGCC TAAGAAACCT 
CGGGACCCTA AAATCCCTAA GAGCAAGCGC CAAAAAAAGG AGCTGGGGGA CAGCTCTGGG 
GAGGGGCCAG AGTTTGTGGA GGAGGAGGAA GAGGTGGCTC TGCGCTCAGA CAGTGAGGGC 
AGCGACTATA CTCCTGGCAA GAAGAAGAAG AAGAAGCTTG GACCTAAGAA AGAGAAGAAG 
AGCAAATCCA AGCGGAAGGA GGAGGAGGAG GAGGAGGATG ATGATGATGA TTCAAAGGAG 
CCTAAATCAT CTGCTCAGCT CCTGGAAGAC TGGGGCATGG AAGACATTGA CCACGTGTTC 
TCAGAGGAGG ATTATCGAAC CCTCACCAAC TACAAGGCCT TCAGCCAGTT TGTCAGACCC 
CTCATTGCTG CCAAAAATCC CAAGATTGCT GTCTCCAAGA TGATGATGGT TTTGGGTGCA 
AAATGGCGGG AGTTCAGTAC CAATAACCCC TTCAAAGGCA GTTCTGGGGC ATCAGTGGCA 
GCTGCGGCAG CAGCAGCGGT AGCTGTGGTG GAGAGCATGG TGACAGCCAC TGAGGTTGCA 
CCACCACCTC CCCCTGTGGA GGTGCCTATC CGCAAGGCCA AGACCAAGGA GGGCAAAGGT 
CCCAATGCTC GGAGGAAGCC CAAGGGCAGC CCTCGTGTAC CTGATGCCAA GAAGCCTAAA 
CCCAAGAAAG TAGCTCCCCT GAAAATCAAG CTGGGAGGTT TTGGTTCCAA GCGTAAGAGA 
TCCTCGAGTG AGGATGATGA CTTAGATGTG GAATCTGACT TCGATGATGC CAGTATCAAT 
AGCTATTCTG TTTCTGATGG TTCCACCAGC CGTAGTAGCC GCAGCCGCAA GAAACTCCGA 
ACCACTAAAA AGAAAAAGAA AGGCGAGGAG GAGGTGACTG CTGTGGATGG TTATGAGACA 
GACCACCAGG ACTATTGCGA GGTGTGCCAG CAAGGCGGTG AGATCATCCT GTGTGATACC 
TGTCCCCGTG CTTACCACAT GGTCTGCCTG GATCCCGACA TGGAGAAGGC TCCCGAGGGC 
AAGTGGAGCT GCCCACACTG CGAGAAGGAA GGCATCCAGT GGGAAGCTAA AGAGGACAAT 
TCGGAGGGTG AGGAGATCCT GGAAGAGGTT GGGGGAGACC TCGAAGAGGA GGATGACCAC 
CATATGGAAT TCTGTCGGGT CTGCAAGGAT GGTGGGGAAC TGCTCTGCTG TGATACCTGT 
CCTTCTTCCT ACCACATCCA CTGCCTGAAT CCCCCACTTC CAGAGATCCC CAACGGTGAA 
TGGCTCTGTC CCCGTTGTAC GTGTCCAGCT CTGAAGGGCA AAGTGCAGAA GATCCTAATC 
TGGAAGTGGG GTCAGCCACC ATCTCCCACA CCAGTGCCTC GGCCTCCAGA TGCTGATCCC 
AACACGCCCT CCCCAAAGCC CTTGGAGGGG CGGCCAGAGC GGCAGTTCTT TGTGAAATGG 
CAAGGCATGT CTTACTGGCA CTGCTCCTGG GTTTCTGAAC TGCAGCTGGA GCTGCACTGT 
CAGGTGATGT TCCGAAACTA TCAGCGGAAG AATGATATGG ATGAGCCACC TTCTGGGGAC 
TTTGGTGGTG ATGAAGAGAA AAGCCGAAAG CGAAAGAACA AGGACCCTAA ATTTGCAGAG 
ATGGAGGAAC GCTTCTATCG CTATGGGATA AAACCCGAGT GGATGATGAT CCACCGAATC 
CTCAACCACA GTGTGGACAA GAAGGGCCAC GTCCACTACT TGATCAAGTG GCGGGACTTA 
CCTTACGATC AGGCTTCTTG GGAGAGTGAG GATGTGGAGA TCCAGGATTA CGACCTGTTC 
AAGCAGAGCT ATTGGAATCA CAGGGAGTTA ATGAGGGGTG AGGAAGGCCG ACCAGGCAAG 
AAGCTCAAGA AGGTGAAGCT TCGGAAGTTG GAGAGGCCTC CAGAAACGCC AACAGTTGAT 
CCAACAGTGA AGTATGAGCG ACAGCCAGAG TACCTGGATG CTACAGGTGG AACCCTGCAC 
CCCTATCAAA TGGAGGGCCT GAATTGGTTG CGCTTCTCCT GGGCTCAGGG CACTGACACC 
ATCTTGGCTG ATGAGATGGG CCTTGGGAAA ACTGTACAGA CAGCAGTCTT CCTGTATTCC 
CTTTACAAGG AGGGTCATTC CAAAGGCCCC TTCCTAGTGA GCGCCCCTCT TTCTACCATC 
ATCAACTGGG AGCGGGAGTT TGAAATGTGG GCTCCAGACA TGTATGTCGT AACCTATGTG 
GGTGACAAGG ACAGCCGTGC CATCATCCGA GAGAATGAGT TCTCCTTTGA AGACAATGCC 
ATTCGTGGTG GCAAGAAGGC CTCCCGCATG AAGAAAGAGG CATCTGTGAA ATTCCATGTG 
CTGCTGACAT CCTATGAATT GATCACCATT GACATGGCTA TTTTGGGCTC TATTGATTGG 
GCCTGCCTCA TCGTGGATGA AGCCCATCGG CTGAAGAACA ATCAGTCTAA GTTCTTCCGG 
GTATTGAATG GTTACTCACT CCAGCACAAG CTGTTGCTGA CTGGGACACC ATTACAAAAC 
AATCTGGAAG AGTTGTTTCA TCTGCTCAAC TTTCTCACCC CCGAGAGGTT CCACAATTTG 
GAAGGTTTTT TGGAGGAGTT TGCTGACATT GCCAAGGAGG ACCAGATAAA AAAACTGCAT 
GACATGCTGG GGCCGCACAT GTTGCGGCGG CTCAAAGCCG ATGTGTTCAA GAACATGCCC 
TCCAAGACAG AACTAATTGT GCGTGTGGAG CTGAGCCCTA TGCAGAAGAA ATACTACAAG 
TACATCCTCA CTCGAAATTT TGAAGCACTC AATGCCCGAG GTGGTGGCAA CCAGGTGTCT 
CTGCTGAATG TGGTGATGGA TCTTAAGAAG TGCTGCAACC ATCCATACCT CTTCCCTGTG 
GCTGCAATGG AAGCTCCTAA GATGCCTAAT GGCATGTATG ATGGCAGTGC CCTAATCAGA 
GCATCTGGGA AATTATTGCT GCTGCAGAAA ATGCTCAAGA ACCTTAAGGA GGGTGGGCAT 
CGTGTACTCA TCTTTTCCCA GATGACCAAG ATGCTAGACC TGCTAGAGGA TTTCTTGGAA 
CATGAAGGTT ATAAATACGA ACGCATCGAT GGTGGAATCA CTGGGAACAT GCGGCAAGAG 
GCCATTGACC GCTTCAATGC ACCGGGTGCT CAGCAGTTCT GCTTCTTGCT TTCCACTCGA 
GCTGGGGGCC TTGGAATCAA TCTGGCCACT GCTGACACAG TTATTATCTA TGACTCTGAC 
TGGAACCCCC ATAATGACAT TCAGGCCTTT AGCAGAGCTC ACCGGATTGG GCAAAATAAA 
AAGGTAATGA TCTACCGGTT TGTGACCCGT GCGTCAGTGG AGGAGCGCAT CACGCAGGTG 
GCAAAGAAGA AAATGATGCT GACGCATCTA GTGGTGCGGC CTGGGCTGGG CTCCAAGACT 
GGATCTATGT CCAAACAGGA GCTTGATGAT ATCCTCAAAT TTGGCACTGA GGAACTATTC 
AAGGATGAAG CCACTGATGG AGGAGGAGAC AACAAAGAGG GAGAAGATAG CAGTGTTATC 
CACTACGATG ATAAGGCCAT TGAACGGCTG CTAGACCGTA ACCAGGATGA GACTGAAGAC 
ACAGAATTGC AGGGCATGAA TGAATATTTG AGCTCATTCA AAGTGGCCCA GTATGTGGTA 
CGGGAAGAAG AAATGGGGGA GGAAGAGGAG GTAGAACGGG AAATCATTAA ACAGGAAGAA 
AGTGTGGATC CTGACTACTG GGAGAAATTG CTGCGGCACC ATTATGAGCA GCAGCAAGAA 
GATCTAGCCC GAAATCTGGG CAAAGGAAAA AGAATCCGTA AACAGGTCAA CTACAATGAT 
GGCTCCCAGG AGGACCGAGA TTGGCAGGAC GACCAGTCCG ACAACCAGTC CGATTACTCA 
GTGGCTTCAG AGGAAGGTGA TGAAGACTTT GATGAACGTT CAGAAGCTCC CCGTAGGCCC 
AGTCGTAAGG GCCTGCGGAA TGATAAAGAT AAGCCATTGC CTCCTCTGTT GGCCCGTGTT 
GGTGGGAATA TTGAAGTACT TGGTTTTAAT GCTCGTCAGC GAAAAGCCTT TCTTAATGCA 
ATTATGCGAT ATGGTATGCC ACCTCAGGAT GCTTTTACTA CCCAGTGGCT TGTAAGAGAC 
CTGCGAGGCA AATCAGAGAA AGAGTTCAAG GCATATGTCT CTCTTTTCAT GCGGCATTTA 
TGTGAGCCGG GGGCAGATGG GGCTGAGACC TTTGCTGATG GTGTCCCCCG AGAAGGCCTG 
TCTCGCCAGC ATGTCCTTAC TAGAATTGGT GTTATGTCTT TGATTCGCAA GAAGGTTCAG 
GAGTTTGAAC ATGTTAATGG GCGCTGGAGC ATGCCTGAAC TGGCTGAGGT GGAGGAAAAC 
AAGAAGATGT CCCAGCCAGG GTCACCCTCC CCAAAAACTC CTACACCCTC CACTCCAGGG 
GACACGCAGC CCAACACTCC TGCACCTGTC CCACCTGCTG AAGATGGGAT AAAAATAGAG 
GAAAATAGCC TCAAAGAAGA AGAGAGCATA GAAGGAGAAA AGGAGGTTAA ATCTACAGCC 
CCTGAGACTG CCATTGAGTG TACACAGGCC CCTGCCCCTG CCTCAGAGGA TGAAAAGGTC 
GTTGTTGAAC CCCCTGAGGG AGAGGAGAAA GTGGAAAAGG CAGAGGTGAA GGAGAGAACA 
GAGGAACCTA TGGAGACAGA GCCCAAAGGT GCTGCTGATG TAGAGAAGGT GGAGGAAAAG 
TCAGCAATAG ATCTGACCCC TATTGTGGTA GAAGACAAAG AAGAGAAGAA AGAAGAAGAA 
GAGAAAAAAG AGGTGATGCT TCAGAATGGA GAGACCCCCA AGGACCTGAA TGATGAGAAA 
CAGAAGAAAA ATATTAAACA ACGTTTCATG TTTAACATTG CAGATGGTGG TTTTACTGAG 
TTGCACTCCC TTTGGCAGAA TGAAGAGCGG GCAGCCACAG TTACCAAGAA GACTTATGAG 
ATCTGGCATC GACGGCATGA CTACTGGCTG CTAGCCGGCA TTATAAACCA TGGCTATGCC 
CGGTGGCAAG ACATCCAGAA TGACCCACGC TATGCCATCC TCAATGAGCC TTTCAAGGGT 
GAAATGAACC GTGGCAATTT CTTAGAGATC AAGAATAAAT TTCTAGCTCG AAGGTTTAAG 
CTCTTAGAAC AAGCTCTGGT GATTGAGGAA CAGCTGCGCC GGGCTGCTTA CTTGAACATG 
TCAGAAGACC CTTCTCACCC TTCCATGGCC CTCAACACCC GCTTTGCTGA GGTGGAGTGT 
TTGGCGGAAA GTCATCAGCA CCTGTCCAAG GAGTCAATGG CAGGAAACAA GCCAGCCAAT 
GCAGTCCTGC ACAAAGGTAT TCTGAAACAG CTGGAAGAAC TGCTGAGTGA CATGAAAGCT 
GATGTGACTC GACTCCCAGC TACCATTGCC CGAATTCCCC CAGTTGCTGT GAGGTTACAG 
ATGTCAGAGC GTAACATTCT CAGCCGCCTG GCAAACCGGG CACCCGAACC TACCCCACAG 
CAGGTAGCCC AGCAGCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHD4 ( XM_017018730.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016874219.1 Homo sapiens chromodomain helicase DNA binding protein 4 (CHD4), transcript variant X16, mRNA.

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CloneID	OHu83475
Clone ID Related Accession (Same CDS sequence)	XM_017018730.1
Accession Version	XM_017018730.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5703bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-25
Organism	Homo sapiens(human)
Product	chromodomain-helicase-DNA-binding protein 4 isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCGG GCCTGGGCTC CCCGTCCCCC TGCTCGGCGG GCAGTGAGGA GGAGGATATG 
GATGCACTTT TGAACAACAG CCTGCCCCCA CCCCACCCAG AAAATGAAGA GGACCCAGAA 
GAGGATTTGT CAGAAACAGA GACTCCAAAG CTCAAGAAGA AGAAAAAGCC TAAGAAACCT 
CGGGACCCTA AAATCCCTAA GAGCAAGCGC CAAAAAAAGG AGCGTATGCT CTTATGCCGG 
CAGCTGGGGG ACAGCTCTGG GGAGGGGCCA GAGTTTGTGG AGGAGGAGGA AGAGGTGGCT 
CTGCGCTCAG ACAGTGAGGG CAGCGACTAT ACTCCTGGCA AGAAGAAGAA GAAGAAGCTT 
GGACCTAAGA AAGAGAAGAA GAGCAAATCC AAGCGGAAGG AGGAGGAGGA GGAGGAGGAT 
GATGATGATG ATTCAAAGGA GCCTAAATCA TCTGCTCAGC TCCTGGAAGA CTGGGGCATG 
GAAGACATTG ACCACGTGTT CTCAGAGGAG GATTATCGAA CCCTCACCAA CTACAAGGCC 
TTCAGCCAGT TTGTCAGACC CCTCATTGCT GCCAAAAATC CCAAGATTGC TGTCTCCAAG 
ATGATGATGG TTTTGGGTGC AAAATGGCGG GAGTTCAGTA CCAATAACCC CTTCAAAGGC 
AGTTCTGGGG CATCAGTGGC AGCTGCGGCA GCAGCAGCGG TAGCTGTGGT GGAGAGCATG 
GTGACAGCCA CTGAGGTTGC ACCACCACCT CCCCCTGTGG AGGTGCCTAT CCGCAAGGCC 
AAGACCAAGG AGGGCAAAGG TCCCAATGCT CGGAGGAAGC CCAAGGGCAG CCCTCGTGTA 
CCTGATGCCA AGAAGCCTAA ACCCAAGAAA GTAGCTCCCC TGAAAATCAA GCTGGGAGGT 
TTTGGTTCCA AGCGTAAGAG ATCCTCGAGT GAGGATGATG ACTTAGATGT GGAATCTGAC 
TTCGATGATG CCAGTATCAA TAGCTATTCT GTTTCTGATG GTTCCACCAG CCGTAGTAGC 
CGCAGCCGCA AGAAACTCCG AACCACTAAA AAGAAAAAGA AAGACCACCA GGACTATTGC 
GAGGTGTGCC AGCAAGGCGG TGAGATCATC CTGTGTGATA CCTGTCCCCG TGCTTACCAC 
ATGGTCTGCC TGGATCCCGA CATGGAGAAG GCTCCCGAGG GCAAGTGGAG CTGCCCACAC 
TGCGAGAAGG AAGGCATCCA GTGGGAAGCT AAAGAGGACA ATTCGGAGGG TGAGGAGATC 
CTGGAAGAGG TTGGGGGAGA CCTCGAAGAG GAGGATGACC ACCATATGGA ATTCTGTCGG 
GTCTGCAAGG ATGGTGGGGA ACTGCTCTGC TGTGATACCT GTCCTTCTTC CTACCACATC 
CACTGCCTGA ATCCCCCACT TCCAGAGATC CCCAACGGTG AATGGCTCTG TCCCCGTTGT 
ACGTGTCCAG CTCTGAAGGG CAAAGTGCAG AAGATCCTAA TCTGGAAGTG GGGTCAGCCA 
CCATCTCCCA CACCAGTGCC TCGGCCTCCA GATGCTGATC CCAACACGCC CTCCCCAAAG 
CCCTTGGAGG GGCGGCCAGA GCGGCAGTTC TTTGTGAAAT GGCAAGGCAT GTCTTACTGG 
CACTGCTCCT GGGTTTCTGA ACTGCAGCTG GAGCTGCACT GTCAGGTGAT GTTCCGAAAC 
TATCAGCGGA AGAATGATAT GGATGAGCCA CCTTCTGGGG ACTTTGGTGG TGATGAAGAG 
AAAAGCCGAA AGCGAAAGAA CAAGGACCCT AAATTTGCAG AGATGGAGGA ACGCTTCTAT 
CGCTATGGGA TAAAACCCGA GTGGATGATG ATCCACCGAA TCCTCAACCA CAGTGTGGAC 
AAGAAGGGCC ACGTCCACTA CTTGATCAAG TGGCGGGACT TACCTTACGA TCAGGCTTCT 
TGGGAGAGTG AGGATGTGGA GATCCAGGAT TACGACCTGT TCAAGCAGAG CTATTGGAAT 
CACAGGGAGT TAATGAGGGG TGAGGAAGGC CGACCAGGCA AGAAGCTCAA GAAGGTGAAG 
CTTCGGAAGT TGGAGAGGCC TCCAGAAACG CCAACAGTTG ATCCAACAGT GAAGTATGAG 
CGACAGCCAG AGTACCTGGA TGCTACAGGT GGAACCCTGC ACCCCTATCA AATGGAGGGC 
CTGAATTGGT TGCGCTTCTC CTGGGCTCAG GGCACTGACA CCATCTTGGC TGATGAGATG 
GGCCTTGGGA AAACTGTACA GACAGCAGTC TTCCTGTATT CCCTTTACAA GGAGGGTCAT 
TCCAAAGGCC CCTTCCTAGT GAGCGCCCCT CTTTCTACCA TCATCAACTG GGAGCGGGAG 
TTTGAAATGT GGGCTCCAGA CATGTATGTC GTAACCTATG TGGGTGACAA GGACAGCCGT 
GCCATCATCC GAGAGAATGA GTTCTCCTTT GAAGACAATG CCATTCGTGG TGGCAAGAAG 
GCCTCCCGCA TGAAGAAAGA GGCATCTGTG AAATTCCATG TGCTGCTGAC ATCCTATGAA 
TTGATCACCA TTGACATGGC TATTTTGGGC TCTATTGATT GGGCCTGCCT CATCGTGGAT 
GAAGCCCATC GGCTGAAGAA CAATCAGTCT AAGTTCTTCC GGGTATTGAA TGGTTACTCA 
CTCCAGCACA AGCTGTTGCT GACTGGGACA CCATTACAAA ACAATCTGGA AGAGTTGTTT 
CATCTGCTCA ACTTTCTCAC CCCCGAGAGG TTCCACAATT TGGAAGGTTT TTTGGAGGAG 
TTTGCTGACA TTGCCAAGGA GGACCAGATA AAAAAACTGC ATGACATGCT GGGGCCGCAC 
ATGTTGCGGC GGCTCAAAGC CGATGTGTTC AAGAACATGC CCTCCAAGAC AGAACTAATT 
GTGCGTGTGG AGCTGAGCCC TATGCAGAAG AAATACTACA AGTACATCCT CACTCGAAAT 
TTTGAAGCAC TCAATGCCCG AGGTGGTGGC AACCAGGTGT CTCTGCTGAA TGTGGTGATG 
GATCTTAAGA AGTGCTGCAA CCATCCATAC CTCTTCCCTG TGGCTGCAAT GGAAGCTCCT 
AAGATGCCTA ATGGCATGTA TGATGGCAGT GCCCTAATCA GAGCATCTGG GAAATTATTG 
CTGCTGCAGA AAATGCTCAA GAACCTTAAG GAGGGTGGGC ATCGTGTACT CATCTTTTCC 
CAGATGACCA AGATGCTAGA CCTGCTAGAG GATTTCTTGG AACATGAAGG TTATAAATAC 
GAACGCATCG ATGGTGGAAT CACTGGGAAC ATGCGGCAAG AGGCCATTGA CCGCTTCAAT 
GCACCGGGTG CTCAGCAGTT CTGCTTCTTG CTTTCCACTC GAGCTGGGGG CCTTGGAATC 
AATCTGGCCA CTGCTGACAC AGTTATTATC TATGACTCTG ACTGGAACCC CCATAATGAC 
ATTCAGGCCT TTAGCAGAGC TCACCGGATT GGGCAAAATA AAAAGGTAAT GATCTACCGG 
TTTGTGACCC GTGCGTCAGT GGAGGAGCGC ATCACGCAGG TGGCAAAGAA GAAAATGATG 
CTGACGCATC TAGTGGTGCG GCCTGGGCTG GGCTCCAAGA CTGGATCTAT GTCCAAACAG 
GAGCTTGATG ATATCCTCAA ATTTGGCACT GAGGAACTAT TCAAGGATGA AGCCACTGAT 
GGAGGAGGAG ACAACAAAGA GGGAGAAGAT AGCAGTGTTA TCCACTACGA TGATAAGGCC 
ATTGAACGGC TGCTAGACCG TAACCAGGAT GAGACTGAAG ACACAGAATT GCAGGGCATG 
AATGAATATT TGAGCTCATT CAAAGTGGCC CAGTATGTGG TACGGGAAGA AGAAATGGGG 
GAGGAAGAGG AGGTAGAACG GGAAATCATT AAACAGGAAG AAAGTGTGGA TCCTGACTAC 
TGGGAGAAAT TGCTGCGGCA CCATTATGAG CAGCAGCAAG AAGATCTAGC CCGAAATCTG 
GGCAAAGGAA AAAGAATCCG TAAACAGGTC AACTACAATG ATGGCTCCCA GGAGGACCGA 
GATTGGCAGG ACGACCAGTC CGACAACCAG TCCGATTACT CAGTGGCTTC AGAGGAAGGT 
GATGAAGACT TTGATGAACG TTCAGAAGCT CCCCGTAGGC CCAGTCGTAA GGGCCTGCGG 
AATGATAAAG ATAAGCCATT GCCTCCTCTG TTGGCCCGTG TTGGTGGGAA TATTGAAGTA 
CTTGGTTTTA ATGCTCGTCA GCGAAAAGCC TTTCTTAATG CAATTATGCG ATATGGTATG 
CCACCTCAGG ATGCTTTTAC TACCCAGTGG CTTGTAAGAG ACCTGCGAGG CAAATCAGAG 
AAAGAGTTCA AGGCATATGT CTCTCTTTTC ATGCGGCATT TATGTGAGCC GGGGGCAGAT 
GGGGCTGAGA CCTTTGCTGA TGGTGTCCCC CGAGAAGGCC TGTCTCGCCA GCATGTCCTT 
ACTAGAATTG GTGTTATGTC TTTGATTCGC AAGAAGGTTC AGGAGTTTGA ACATGTTAAT 
GGGCGCTGGA GCATGCCTGA ACTGGCTGAG GTGGAGGAAA ACAAGAAGAT GTCCCAGCCA 
GGGTCACCCT CCCCAAAAAC TCCTACACCC TCCACTCCAG GGGACACGCA GCCCAACACT 
CCTGCACCTG TCCCACCTGC TGAAGATGGG ATAAAAATAG AGGAAAATAG CCTCAAAGAA 
GAAGAGAGCA TAGAAGGAGA AAAGGAGGTT AAATCTACAG CCCCTGAGAC TGCCATTGAG 
TGTACACAGG CCCCTGCCCC TGCCTCAGAG GATGAAAAGG TCGTTGTTGA ACCCCCTGAG 
GGAGAGGAGA AAGTGGAAAA GGCAGAGGTG AAGGAGAGAA CAGAGGAACC TATGGAGACA 
GAGCCCAAAG GTGCTGCTGA TGTAGAGAAG GTGGAGGAAA AGTCAGCAAT AGATCTGACC 
CCTATTGTGG TAGAAGACAA AGAAGAGAAG AAAGAAGAAG AAGAGAAAAA AGAGGTGATG 
CTTCAGAATG GAGAGACCCC CAAGGACCTG AATGATGAGA AACAGAAGAA AAATATTAAA 
CAACGTTTCA TGTTTAACAT TGCAGATGGT GGTTTTACTG AGTTGCACTC CCTTTGGCAG 
AATGAAGAGC GGGCAGCCAC AGTTACCAAG AAGACTTATG AGATCTGGCA TCGACGGCAT 
GACTACTGGC TGCTAGCCGG CATTATAAAC CATGGCTATG CCCGGTGGCA AGACATCCAG 
AATGACCCAC GCTATGCCAT CCTCAATGAG CCTTTCAAGG GTGAAATGAA CCGTGGCAAT 
TTCTTAGAGA TCAAGAATAA ATTTCTAGCT CGAAGGTTTA AGCTCTTAGA ACAAGCTCTG 
GTGATTGAGG AACAGCTGCG CCGGGCTGCT TACTTGAACA TGTCAGAAGA CCCTTCTCAC 
CCTTCCATGG CCCTCAACAC CCGCTTTGCT GAGGTGGAGT GTTTGGCGGA AAGTCATCAG 
CACCTGTCCA AGGAGTCAAT GGCAGGAAAC AAGCCAGCCA ATGCAGTCCT GCACAAAGGT 
ATTCTGAAAC AGCTGGAAGA ACTGCTGAGT GACATGAAAG CTGATGTGAC TCGACTCCCA 
GCTACCATTG CCCGAATTCC CCCAGTTGCT GTGAGGTTAC AGATGTCAGA GCGTAACATT 
CTCAGCCGCC TGGCAAACCG GGCACCCGAA CCTACCCCAC AGCAGGTAGC CCAGCAGCAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016874219.1
CDS	219..5921
Translation	MASGLGSPSPCSAGSEEEDMDALLNNSLPPPHPENEEDPEEDLSETETPKLKKKKKPKKPRDPKIPKSKRQKKERMLLCRQLGDSSGEGPEFVEEEEEVALRSDSEGSDYTPGKKKKKKLGPKKEKKSKSKRKEEEEEEDDDDDSKEPKSSAQLLEDWGMEDIDHVFSEEDYRTLTNYKAFSQFVRPLIAAKNPKIAVSKMMMVLGAKWREFSTNNPFKGSSGASVAAAAAAAVAVVESMVTATEVAPPPPPVEVPIRKAKTKEGKGPNARRKPKGSPRVPDAKKPKPKKVAPLKIKLGGFGSKRKRSSSEDDDLDVESDFDDASINSYSVSDGSTSRSSRSRKKLRTTKKKKKDHQDYCEVCQQGGEIILCDTCPRAYHMVCLDPDMEKAPEGKWSCPHCEKEGIQWEAKEDNSEGEEILEEVGGDLEEEDDHHMEFCRVCKDGGELLCCDTCPSSYHIHCLNPPLPEIPNGEWLCPRCTCPALKGKVQKILIWKWGQPPSPTPVPRPPDADPNTPSPKPLEGRPERQFFVKWQGMSYWHCSWVSELQLELHCQVMFRNYQRKNDMDEPPSGDFGGDEEKSRKRKNKDPKFAEMEERFYRYGIKPEWMMIHRILNHSVDKKGHVHYLIKWRDLPYDQASWESEDVEIQDYDLFKQSYWNHRELMRGEEGRPGKKLKKVKLRKLERPPETPTVDPTVKYERQPEYLDATGGTLHPYQMEGLNWLRFSWAQGTDTILADEMGLGKTVQTAVFLYSLYKEGHSKGPFLVSAPLSTIINWEREFEMWAPDMYVVTYVGDKDSRAIIRENEFSFEDNAIRGGKKASRMKKEASVKFHVLLTSYELITIDMAILGSIDWACLIVDEAHRLKNNQSKFFRVLNGYSLQHKLLLTGTPLQNNLEELFHLLNFLTPERFHNLEGFLEEFADIAKEDQIKKLHDMLGPHMLRRLKADVFKNMPSKTELIVRVELSPMQKKYYKYILTRNFEALNARGGGNQVSLLNVVMDLKKCCNHPYLFPVAAMEAPKMPNGMYDGSALIRASGKLLLLQKMLKNLKEGGHRVLIFSQMTKMLDLLEDFLEHEGYKYERIDGGITGNMRQEAIDRFNAPGAQQFCFLLSTRAGGLGINLATADTVIIYDSDWNPHNDIQAFSRAHRIGQNKKVMIYRFVTRASVEERITQVAKKKMMLTHLVVRPGLGSKTGSMSKQELDDILKFGTEELFKDEATDGGGDNKEGEDSSVIHYDDKAIERLLDRNQDETEDTELQGMNEYLSSFKVAQYVVREEEMGEEEEVEREIIKQEESVDPDYWEKLLRHHYEQQQEDLARNLGKGKRIRKQVNYNDGSQEDRDWQDDQSDNQSDYSVASEEGDEDFDERSEAPRRPSRKGLRNDKDKPLPPLLARVGGNIEVLGFNARQRKAFLNAIMRYGMPPQDAFTTQWLVRDLRGKSEKEFKAYVSLFMRHLCEPGADGAETFADGVPREGLSRQHVLTRIGVMSLIRKKVQEFEHVNGRWSMPELAEVEENKKMSQPGSPSPKTPTPSTPGDTQPNTPAPVPPAEDGIKIEENSLKEEESIEGEKEVKSTAPETAIECTQAPAPASEDEKVVVEPPEGEEKVEKAEVKERTEEPMETEPKGAADVEKVEEKSAIDLTPIVVEDKEEKKEEEEKKEVMLQNGETPKDLNDEKQKKNIKQRFMFNIADGGFTELHSLWQNEERAATVTKKTYEIWHRRHDYWLLAGIINHGYARWQDIQNDPRYAILNEPFKGEMNRGNFLEIKNKFLARRFKLLEQALVIEEQLRRAAYLNMSEDPSHPSMALNTRFAEVECLAESHQHLSKESMAGNKPANAVLHKGILKQLEELLSDMKADVTRLPATIARIPPVAVRLQMSERNILSRLANRAPEPTPQQVAQQQ

Target ORF information:

RefSeq Version	XM_017018730.1
Organism	Homo sapiens(human)
Definition	Homo sapiens chromodomain helicase DNA binding protein 4 (CHD4), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017018730.1

ORF Insert Sequence:

ATGGCGTCGG GCCTGGGCTC CCCGTCCCCC TGCTCGGCGG GCAGTGAGGA GGAGGATATG 
GATGCACTTT TGAACAACAG CCTGCCCCCA CCCCACCCAG AAAATGAAGA GGACCCAGAA 
GAGGATTTGT CAGAAACAGA GACTCCAAAG CTCAAGAAGA AGAAAAAGCC TAAGAAACCT 
CGGGACCCTA AAATCCCTAA GAGCAAGCGC CAAAAAAAGG AGCGTATGCT CTTATGCCGG 
CAGCTGGGGG ACAGCTCTGG GGAGGGGCCA GAGTTTGTGG AGGAGGAGGA AGAGGTGGCT 
CTGCGCTCAG ACAGTGAGGG CAGCGACTAT ACTCCTGGCA AGAAGAAGAA GAAGAAGCTT 
GGACCTAAGA AAGAGAAGAA GAGCAAATCC AAGCGGAAGG AGGAGGAGGA GGAGGAGGAT 
GATGATGATG ATTCAAAGGA GCCTAAATCA TCTGCTCAGC TCCTGGAAGA CTGGGGCATG 
GAAGACATTG ACCACGTGTT CTCAGAGGAG GATTATCGAA CCCTCACCAA CTACAAGGCC 
TTCAGCCAGT TTGTCAGACC CCTCATTGCT GCCAAAAATC CCAAGATTGC TGTCTCCAAG 
ATGATGATGG TTTTGGGTGC AAAATGGCGG GAGTTCAGTA CCAATAACCC CTTCAAAGGC 
AGTTCTGGGG CATCAGTGGC AGCTGCGGCA GCAGCAGCGG TAGCTGTGGT GGAGAGCATG 
GTGACAGCCA CTGAGGTTGC ACCACCACCT CCCCCTGTGG AGGTGCCTAT CCGCAAGGCC 
AAGACCAAGG AGGGCAAAGG TCCCAATGCT CGGAGGAAGC CCAAGGGCAG CCCTCGTGTA 
CCTGATGCCA AGAAGCCTAA ACCCAAGAAA GTAGCTCCCC TGAAAATCAA GCTGGGAGGT 
TTTGGTTCCA AGCGTAAGAG ATCCTCGAGT GAGGATGATG ACTTAGATGT GGAATCTGAC 
TTCGATGATG CCAGTATCAA TAGCTATTCT GTTTCTGATG GTTCCACCAG CCGTAGTAGC 
CGCAGCCGCA AGAAACTCCG AACCACTAAA AAGAAAAAGA AAGACCACCA GGACTATTGC 
GAGGTGTGCC AGCAAGGCGG TGAGATCATC CTGTGTGATA CCTGTCCCCG TGCTTACCAC 
ATGGTCTGCC TGGATCCCGA CATGGAGAAG GCTCCCGAGG GCAAGTGGAG CTGCCCACAC 
TGCGAGAAGG AAGGCATCCA GTGGGAAGCT AAAGAGGACA ATTCGGAGGG TGAGGAGATC 
CTGGAAGAGG TTGGGGGAGA CCTCGAAGAG GAGGATGACC ACCATATGGA ATTCTGTCGG 
GTCTGCAAGG ATGGTGGGGA ACTGCTCTGC TGTGATACCT GTCCTTCTTC CTACCACATC 
CACTGCCTGA ATCCCCCACT TCCAGAGATC CCCAACGGTG AATGGCTCTG TCCCCGTTGT 
ACGTGTCCAG CTCTGAAGGG CAAAGTGCAG AAGATCCTAA TCTGGAAGTG GGGTCAGCCA 
CCATCTCCCA CACCAGTGCC TCGGCCTCCA GATGCTGATC CCAACACGCC CTCCCCAAAG 
CCCTTGGAGG GGCGGCCAGA GCGGCAGTTC TTTGTGAAAT GGCAAGGCAT GTCTTACTGG 
CACTGCTCCT GGGTTTCTGA ACTGCAGCTG GAGCTGCACT GTCAGGTGAT GTTCCGAAAC 
TATCAGCGGA AGAATGATAT GGATGAGCCA CCTTCTGGGG ACTTTGGTGG TGATGAAGAG 
AAAAGCCGAA AGCGAAAGAA CAAGGACCCT AAATTTGCAG AGATGGAGGA ACGCTTCTAT 
CGCTATGGGA TAAAACCCGA GTGGATGATG ATCCACCGAA TCCTCAACCA CAGTGTGGAC 
AAGAAGGGCC ACGTCCACTA CTTGATCAAG TGGCGGGACT TACCTTACGA TCAGGCTTCT 
TGGGAGAGTG AGGATGTGGA GATCCAGGAT TACGACCTGT TCAAGCAGAG CTATTGGAAT 
CACAGGGAGT TAATGAGGGG TGAGGAAGGC CGACCAGGCA AGAAGCTCAA GAAGGTGAAG 
CTTCGGAAGT TGGAGAGGCC TCCAGAAACG CCAACAGTTG ATCCAACAGT GAAGTATGAG 
CGACAGCCAG AGTACCTGGA TGCTACAGGT GGAACCCTGC ACCCCTATCA AATGGAGGGC 
CTGAATTGGT TGCGCTTCTC CTGGGCTCAG GGCACTGACA CCATCTTGGC TGATGAGATG 
GGCCTTGGGA AAACTGTACA GACAGCAGTC TTCCTGTATT CCCTTTACAA GGAGGGTCAT 
TCCAAAGGCC CCTTCCTAGT GAGCGCCCCT CTTTCTACCA TCATCAACTG GGAGCGGGAG 
TTTGAAATGT GGGCTCCAGA CATGTATGTC GTAACCTATG TGGGTGACAA GGACAGCCGT 
GCCATCATCC GAGAGAATGA GTTCTCCTTT GAAGACAATG CCATTCGTGG TGGCAAGAAG 
GCCTCCCGCA TGAAGAAAGA GGCATCTGTG AAATTCCATG TGCTGCTGAC ATCCTATGAA 
TTGATCACCA TTGACATGGC TATTTTGGGC TCTATTGATT GGGCCTGCCT CATCGTGGAT 
GAAGCCCATC GGCTGAAGAA CAATCAGTCT AAGTTCTTCC GGGTATTGAA TGGTTACTCA 
CTCCAGCACA AGCTGTTGCT GACTGGGACA CCATTACAAA ACAATCTGGA AGAGTTGTTT 
CATCTGCTCA ACTTTCTCAC CCCCGAGAGG TTCCACAATT TGGAAGGTTT TTTGGAGGAG 
TTTGCTGACA TTGCCAAGGA GGACCAGATA AAAAAACTGC ATGACATGCT GGGGCCGCAC 
ATGTTGCGGC GGCTCAAAGC CGATGTGTTC AAGAACATGC CCTCCAAGAC AGAACTAATT 
GTGCGTGTGG AGCTGAGCCC TATGCAGAAG AAATACTACA AGTACATCCT CACTCGAAAT 
TTTGAAGCAC TCAATGCCCG AGGTGGTGGC AACCAGGTGT CTCTGCTGAA TGTGGTGATG 
GATCTTAAGA AGTGCTGCAA CCATCCATAC CTCTTCCCTG TGGCTGCAAT GGAAGCTCCT 
AAGATGCCTA ATGGCATGTA TGATGGCAGT GCCCTAATCA GAGCATCTGG GAAATTATTG 
CTGCTGCAGA AAATGCTCAA GAACCTTAAG GAGGGTGGGC ATCGTGTACT CATCTTTTCC 
CAGATGACCA AGATGCTAGA CCTGCTAGAG GATTTCTTGG AACATGAAGG TTATAAATAC 
GAACGCATCG ATGGTGGAAT CACTGGGAAC ATGCGGCAAG AGGCCATTGA CCGCTTCAAT 
GCACCGGGTG CTCAGCAGTT CTGCTTCTTG CTTTCCACTC GAGCTGGGGG CCTTGGAATC 
AATCTGGCCA CTGCTGACAC AGTTATTATC TATGACTCTG ACTGGAACCC CCATAATGAC 
ATTCAGGCCT TTAGCAGAGC TCACCGGATT GGGCAAAATA AAAAGGTAAT GATCTACCGG 
TTTGTGACCC GTGCGTCAGT GGAGGAGCGC ATCACGCAGG TGGCAAAGAA GAAAATGATG 
CTGACGCATC TAGTGGTGCG GCCTGGGCTG GGCTCCAAGA CTGGATCTAT GTCCAAACAG 
GAGCTTGATG ATATCCTCAA ATTTGGCACT GAGGAACTAT TCAAGGATGA AGCCACTGAT 
GGAGGAGGAG ACAACAAAGA GGGAGAAGAT AGCAGTGTTA TCCACTACGA TGATAAGGCC 
ATTGAACGGC TGCTAGACCG TAACCAGGAT GAGACTGAAG ACACAGAATT GCAGGGCATG 
AATGAATATT TGAGCTCATT CAAAGTGGCC CAGTATGTGG TACGGGAAGA AGAAATGGGG 
GAGGAAGAGG AGGTAGAACG GGAAATCATT AAACAGGAAG AAAGTGTGGA TCCTGACTAC 
TGGGAGAAAT TGCTGCGGCA CCATTATGAG CAGCAGCAAG AAGATCTAGC CCGAAATCTG 
GGCAAAGGAA AAAGAATCCG TAAACAGGTC AACTACAATG ATGGCTCCCA GGAGGACCGA 
GATTGGCAGG ACGACCAGTC CGACAACCAG TCCGATTACT CAGTGGCTTC AGAGGAAGGT 
GATGAAGACT TTGATGAACG TTCAGAAGCT CCCCGTAGGC CCAGTCGTAA GGGCCTGCGG 
AATGATAAAG ATAAGCCATT GCCTCCTCTG TTGGCCCGTG TTGGTGGGAA TATTGAAGTA 
CTTGGTTTTA ATGCTCGTCA GCGAAAAGCC TTTCTTAATG CAATTATGCG ATATGGTATG 
CCACCTCAGG ATGCTTTTAC TACCCAGTGG CTTGTAAGAG ACCTGCGAGG CAAATCAGAG 
AAAGAGTTCA AGGCATATGT CTCTCTTTTC ATGCGGCATT TATGTGAGCC GGGGGCAGAT 
GGGGCTGAGA CCTTTGCTGA TGGTGTCCCC CGAGAAGGCC TGTCTCGCCA GCATGTCCTT 
ACTAGAATTG GTGTTATGTC TTTGATTCGC AAGAAGGTTC AGGAGTTTGA ACATGTTAAT 
GGGCGCTGGA GCATGCCTGA ACTGGCTGAG GTGGAGGAAA ACAAGAAGAT GTCCCAGCCA 
GGGTCACCCT CCCCAAAAAC TCCTACACCC TCCACTCCAG GGGACACGCA GCCCAACACT 
CCTGCACCTG TCCCACCTGC TGAAGATGGG ATAAAAATAG AGGAAAATAG CCTCAAAGAA 
GAAGAGAGCA TAGAAGGAGA AAAGGAGGTT AAATCTACAG CCCCTGAGAC TGCCATTGAG 
TGTACACAGG CCCCTGCCCC TGCCTCAGAG GATGAAAAGG TCGTTGTTGA ACCCCCTGAG 
GGAGAGGAGA AAGTGGAAAA GGCAGAGGTG AAGGAGAGAA CAGAGGAACC TATGGAGACA 
GAGCCCAAAG GTGCTGCTGA TGTAGAGAAG GTGGAGGAAA AGTCAGCAAT AGATCTGACC 
CCTATTGTGG TAGAAGACAA AGAAGAGAAG AAAGAAGAAG AAGAGAAAAA AGAGGTGATG 
CTTCAGAATG GAGAGACCCC CAAGGACCTG AATGATGAGA AACAGAAGAA AAATATTAAA 
CAACGTTTCA TGTTTAACAT TGCAGATGGT GGTTTTACTG AGTTGCACTC CCTTTGGCAG 
AATGAAGAGC GGGCAGCCAC AGTTACCAAG AAGACTTATG AGATCTGGCA TCGACGGCAT 
GACTACTGGC TGCTAGCCGG CATTATAAAC CATGGCTATG CCCGGTGGCA AGACATCCAG 
AATGACCCAC GCTATGCCAT CCTCAATGAG CCTTTCAAGG GTGAAATGAA CCGTGGCAAT 
TTCTTAGAGA TCAAGAATAA ATTTCTAGCT CGAAGGTTTA AGCTCTTAGA ACAAGCTCTG 
GTGATTGAGG AACAGCTGCG CCGGGCTGCT TACTTGAACA TGTCAGAAGA CCCTTCTCAC 
CCTTCCATGG CCCTCAACAC CCGCTTTGCT GAGGTGGAGT GTTTGGCGGA AAGTCATCAG 
CACCTGTCCA AGGAGTCAAT GGCAGGAAAC AAGCCAGCCA ATGCAGTCCT GCACAAAGGT 
ATTCTGAAAC AGCTGGAAGA ACTGCTGAGT GACATGAAAG CTGATGTGAC TCGACTCCCA 
GCTACCATTG CCCGAATTCC CCCAGTTGCT GTGAGGTTAC AGATGTCAGA GCGTAACATT 
CTCAGCCGCC TGGCAAACCG GGCACCCGAA CCTACCCCAC AGCAGGTAGC CCAGCAGCAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHD4 ( XM_017018731.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016874220.1 Homo sapiens chromodomain helicase DNA binding protein 4 (CHD4), transcript variant X17, mRNA.

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CloneID	OHu83476
Clone ID Related Accession (Same CDS sequence)	XM_017018731.1
Accession Version	XM_017018731.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5700bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-25
Organism	Homo sapiens(human)
Product	chromodomain-helicase-DNA-binding protein 4 isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCGG GCCTGGGCTC CCCGTCCCCC TGCTCGGCGG GCAGTGAGGA GGAGGATATG 
GATGCACTTT TGAACAACAG CCTGCCCCCA CCCCACCCAG AAAATGAAGA GGACCCAGAA 
GAGGATTTGT CAGAAACAGA GACTCCAAAG CTCAAGAAGA AGAAAAAGCC TAAGAAACCT 
CGGGACCCTA AAATCCCTAA GAGCAAGCGC CAAAAAAAGG AGCGTATGCT CTTATGCCGG 
CAGCTGGGGG ACAGCTCTGG GGAGGGGCCA GAGTTTGTGG AGGAGGAGGA AGAGGTGGCT 
CTGCGCTCAG ACAGTGAGGG CAGCGACTAT ACTCCTGGCA AGAAGAAGAA GAAGAAGCTT 
GGACCTAAGA AAGAGAAGAA GAGCAAATCC AAGCGGAAGG AGGAGGAGGA GGAGGAGGAT 
GATGATGATG ATTCAAAGGA GCCTAAATCA TCTGCTCAGC TCCTGGAAGA CTGGGGCATG 
GAAGACATTG ACCACGTGTT CTCAGAGGAG GATTATCGAA CCCTCACCAA CTACAAGGCC 
TTCAGCCAGT TTGTCAGACC CCTCATTGCT GCCAAAAATC CCAAGATTGC TGTCTCCAAG 
ATGATGATGG TTTTGGGTGC AAAATGGCGG GAGTTCAGTA CCAATAACCC CTTCAAAGGC 
AGTTCTGGGG CATCAGTGGC AGCTGCGGCA GCAGCAGCGG TAGCTGTGGT GGAGAGCATG 
GTGACAGCCA CTGAGGTTGC ACCACCACCT CCCCCTGTGG AGGTGCCTAT CCGCAAGGCC 
AAGACCAAGG AGGGCAAAGG TCCCAATGCT CGGAGGAAGC CCAAGGGCAG CCCTCGTGTA 
CCTGATGCCA AGAAGCCTAA ACCCAAGAAA GTAGCTCCCC TGAAAATCAA GCTGGGAGGT 
TTTGGTTCCA AGCGTAAGAG ATCCTCGAGT GAGGATGATG ACTTAGATGT GGAATCTGAC 
TTCGATGATG CCAGTATCAA TAGCTATTCT GTTTCTGATG GTTCCACCAG CCGTAGTAGC 
CGCAGCCGCA AGAAACTCCG AACCACTAAA AAGAAAAAGA AAGACCACCA GGACTATTGC 
GAGGTGTGCC AGCAAGGCGG TGAGATCATC CTGTGTGATA CCTGTCCCCG TGCTTACCAC 
ATGGTCTGCC TGGATCCCGA CATGGAGAAG GCTCCCGAGG GCAAGTGGAG CTGCCCACAC 
TGCGAGAAGG AAGGCATCCA GTGGGAAGCT AAAGAGGACA ATTCGGAGGG TGAGGAGATC 
CTGGAAGAGG TTGGGGGAGA CCTCGAAGAG GAGGATGACC ACCATATGGA ATTCTGTCGG 
GTCTGCAAGG ATGGTGGGGA ACTGCTCTGC TGTGATACCT GTCCTTCTTC CTACCACATC 
CACTGCCTGA ATCCCCCACT TCCAGAGATC CCCAACGGTG AATGGCTCTG TCCCCGTTGT 
ACGTGTCCAG CTCTGAAGGG CAAAGTGCAG AAGATCCTAA TCTGGAAGTG GGGTCAGCCA 
CCATCTCCCA CACCAGTGCC TCGGCCTCCA GATGCTGATC CCAACACGCC CTCCCCAAAG 
CCCTTGGAGG GGCGGCCAGA GCGGCAGTTC TTTGTGAAAT GGCAAGGCAT GTCTTACTGG 
CACTGCTCCT GGGTTTCTGA ACTGCAGCTG GAGCTGCACT GTCAGGTGAT GTTCCGAAAC 
TATCAGCGGA AGAATGATAT GGATGAGCCA CCTTCTGGGG ACTTTGGTGG TGATGAAGAG 
AAAAGCCGAA AGCGAAAGAA CAAGGACCCT AAATTTGCAG AGATGGAGGA ACGCTTCTAT 
CGCTATGGGA TAAAACCCGA GTGGATGATG ATCCACCGAA TCCTCAACCA CAGTGTGGAC 
AAGAAGGGCC ACGTCCACTA CTTGATCAAG TGGCGGGACT TACCTTACGA TCAGGCTTCT 
TGGGAGAGTG AGGATGTGGA GATCCAGGAT TACGACCTGT TCAAGCAGAG CTATTGGAAT 
CACAGGGAGT TAATGAGGGG TGAGGAAGGC CGACCAGGCA AGAAGCTCAA GAAGGTGAAG 
CTTCGGAAGT TGGAGAGGCC TCCAGAAACG CCAACAGTTG ATCCAACAGT GAAGTATGAG 
CGACAGCCAG AGTACCTGGA TGCTACAGGT GGAACCCTGC ACCCCTATCA AATGGAGGGC 
CTGAATTGGT TGCGCTTCTC CTGGGCTCAG GGCACTGACA CCATCTTGGC TGATGAGATG 
GGCCTTGGGA AAACTGTACA GACAGCAGTC TTCCTGTATT CCCTTTACAA GGAGGGTCAT 
TCCAAAGGCC CCTTCCTAGT GAGCGCCCCT CTTTCTACCA TCATCAACTG GGAGCGGGAG 
TTTGAAATGT GGGCTCCAGA CATGTATGTC GTAACCTATG TGGGTGACAA GGACAGCCGT 
GCCATCATCC GAGAGAATGA GTTCTCCTTT GAAGACAATG CCATTCGTGG TGGCAAGAAG 
GCCTCCCGCA TGAAGAAAGA GGCATCTGTG AAATTCCATG TGCTGCTGAC ATCCTATGAA 
TTGATCACCA TTGACATGGC TATTTTGGGC TCTATTGATT GGGCCTGCCT CATCGTGGAT 
GAAGCCCATC GGCTGAAGAA CAATCAGTCT AAGTTCTTCC GGGTATTGAA TGGTTACTCA 
CTCCAGCACA AGCTGTTGCT GACTGGGACA CCATTACAAA ACAATCTGGA AGAGTTGTTT 
CATCTGCTCA ACTTTCTCAC CCCCGAGAGG TTCCACAATT TGGAAGGTTT TTTGGAGGAG 
TTTGCTGACA TTGCCAAGGA GGACCAGATA AAAAAACTGC ATGACATGCT GGGGCCGCAC 
ATGTTGCGGC GGCTCAAAGC CGATGTGTTC AAGAACATGC CCTCCAAGAC AGAACTAATT 
GTGCGTGTGG AGCTGAGCCC TATGCAGAAG AAATACTACA AGTACATCCT CACTCGAAAT 
TTTGAAGCAC TCAATGCCCG AGGTGGTGGC AACCAGGTGT CTCTGCTGAA TGTGGTGATG 
GATCTTAAGA AGTGCTGCAA CCATCCATAC CTCTTCCCTG TGGCTGCAAT GGAAGCTCCT 
AAGATGCCTA ATGGCATGTA TGATGGCAGT GCCCTAATCA GAGCATCTGG GAAATTATTG 
CTGCTGCAGA AAATGCTCAA GAACCTTAAG GAGGGTGGGC ATCGTGTACT CATCTTTTCC 
CAGATGACCA AGATGCTAGA CCTGCTAGAG GATTTCTTGG AACATGAAGG TTATAAATAC 
GAACGCATCG ATGGTGGAAT CACTGGGAAC ATGCGGCAAG AGGCCATTGA CCGCTTCAAT 
GCACCGGGTG CTCAGCAGTT CTGCTTCTTG CTTTCCACTC GAGCTGGGGG CCTTGGAATC 
AATCTGGCCA CTGCTGACAC AGTTATTATC TATGACTCTG ACTGGAACCC CCATAATGAC 
ATTCAGGCCT TTAGCAGAGC TCACCGGATT GGGCAAAATA AAAAGGTAAT GATCTACCGG 
TTTGTGACCC GTGCGTCAGT GGAGGAGCGC ATCACGCAGG TGGCAAAGAA GAAAATGATG 
CTGACGCATC TAGTGGTGCG GCCTGGGCTG GGCTCCAAGA CTGGATCTAT GTCCAAACAG 
GAGCTTGATG ATATCCTCAA ATTTGGCACT GAGGAACTAT TCAAGGATGA AGCCACTGAT 
GGAGGAGGAG ACAACAAAGA GGGAGAAGAT AGCAGTGTTA TCCACTACGA TGATAAGGCC 
ATTGAACGGC TGCTAGACCG TAACCAGGAT GAGACTGAAG ACACAGAATT GCAGGGCATG 
AATGAATATT TGAGCTCATT CAAAGTGGCC CAGTATGTGG TACGGGAAGA AGAAATGGGG 
GAGGAAGAGG AGGTAGAACG GGAAATCATT AAACAGGAAG AAAGTGTGGA TCCTGACTAC 
TGGGAGAAAT TGCTGCGGCA CCATTATGAG CAGCAGCAAG AAGATCTAGC CCGAAATCTG 
GGCAAAGGAA AAAGAATCCG TAAACAGGTC AACTACAATG ATGGCTCCCA GGAGGACCGA 
GATTGGCAGG ACGACCAGTC CGACAACCAG TCCGATTACT CAGTGGCTTC AGAGGAAGGT 
GATGAAGACT TTGATGAACG TTCAGAAGCT CCCCGTAGGC CCAGTCGTAA GGGCCTGCGG 
AATGATAAAG ATAAGCCATT GCCTCCTCTG TTGGCCCGTG TTGGTGGGAA TATTGAAGTA 
CTTGGTTTTA ATGCTCGTCA GCGAAAAGCC TTTCTTAATG CAATTATGCG ATATGGTATG 
CCACCTCAGG ATGCTTTTAC TACCCAGTGG CTTGTAAGAG ACCTGCGAGG CAAATCAGAG 
AAAGAGTTCA AGGCATATGT CTCTCTTTTC ATGCGGCATT TATGTGAGCC GGGGGCAGAT 
GGGGCTGAGA CCTTTGCTGA TGGTGTCCCC CGAGAAGGCC TGTCTCGCCA GCATGTCCTT 
ACTAGAATTG GTGTTATGTC TTTGATTCGC AAGAAGGTTC AGGAGTTTGA ACATGTTAAT 
GGGCGCTGGA GCATGCCTGA ACTGGCTGAG GTGGAGGAAA ACAAGAAGAT GTCCCAGCCA 
GGGTCACCCT CCCCAAAAAC TCCTACACCC TCCACTCCAG GGGACACGCA GCCCAACACT 
CCTGCACCTG TCCCACCTGC TGAAGATGGG ATAAAAATAG AGGAAAATAG CCTCAAAGAA 
GAAGAGAGCA TAGAAGGAGA AAAGGAGGTT AAATCTACAG CCCCTGAGAC TGCCATTGAG 
TGTACACAGG CCCCTGCCCC TGCCTCAGAG GATGAAAAGG TCGTTGTTGA ACCCCCTGAG 
GGAGAGGAGA AAGTGGAAAA GGCAGAGGTG AAGGAGAGAA CAGAGGAACC TATGGAGACA 
GAGCCCAAAG GTGCTGCTGA TGTAGAGAAG GTGGAGGAAA AGTCAGCAAT AGATCTGACC 
CCTATTGTGG TAGAAGACAA AGAAGAGAAG AAAGAAGAAG AAGAGAAAAA AGAGGTGATG 
CTTCAGAATG GAGAGACCCC CAAGGACCTG AATGATGAGA AACAGAAGAA AAATATTAAA 
CAACGTTTCA TGTTTAACAT TGCAGATGGT GGTTTTACTG AGTTGCACTC CCTTTGGCAG 
AATGAAGAGC GGGCAGCCAC AGTTACCAAG AAGACTTATG AGATCTGGCA TCGACGGCAT 
GACTACTGGC TGCTAGCCGG CATTATAAAC CATGGCTATG CCCGGTGGCA AGACATCCAG 
AATGACCCAC GCTATGCCAT CCTCAATGAG CCTTTCAAGG GTGAAATGAA CCGTGGCAAT 
TTCTTAGAGA TCAAGAATAA ATTTCTAGCT CGAAGGTTTA AGCTCTTAGA ACAAGCTCTG 
GTGATTGAGG AACAGCTGCG CCGGGCTGCT TACTTGAACA TGTCAGAAGA CCCTTCTCAC 
CCTTCCATGG CCCTCAACAC CCGCTTTGCT GAGGTGGAGT GTTTGGCGGA AAGTCATCAG 
CACCTGTCCA AGGAGTCAAT GGCAGGAAAC AAGCCAGCCA ATGCAGTCCT GCACAAAGTT 
CTGAAACAGC TGGAAGAACT GCTGAGTGAC ATGAAAGCTG ATGTGACTCG ACTCCCAGCT 
ACCATTGCCC GAATTCCCCC AGTTGCTGTG AGGTTACAGA TGTCAGAGCG TAACATTCTC 
AGCCGCCTGG CAAACCGGGC ACCCGAACCT ACCCCACAGC AGGTAGCCCA GCAGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016874220.1
CDS	219..5918
Translation	MASGLGSPSPCSAGSEEEDMDALLNNSLPPPHPENEEDPEEDLSETETPKLKKKKKPKKPRDPKIPKSKRQKKERMLLCRQLGDSSGEGPEFVEEEEEVALRSDSEGSDYTPGKKKKKKLGPKKEKKSKSKRKEEEEEEDDDDDSKEPKSSAQLLEDWGMEDIDHVFSEEDYRTLTNYKAFSQFVRPLIAAKNPKIAVSKMMMVLGAKWREFSTNNPFKGSSGASVAAAAAAAVAVVESMVTATEVAPPPPPVEVPIRKAKTKEGKGPNARRKPKGSPRVPDAKKPKPKKVAPLKIKLGGFGSKRKRSSSEDDDLDVESDFDDASINSYSVSDGSTSRSSRSRKKLRTTKKKKKDHQDYCEVCQQGGEIILCDTCPRAYHMVCLDPDMEKAPEGKWSCPHCEKEGIQWEAKEDNSEGEEILEEVGGDLEEEDDHHMEFCRVCKDGGELLCCDTCPSSYHIHCLNPPLPEIPNGEWLCPRCTCPALKGKVQKILIWKWGQPPSPTPVPRPPDADPNTPSPKPLEGRPERQFFVKWQGMSYWHCSWVSELQLELHCQVMFRNYQRKNDMDEPPSGDFGGDEEKSRKRKNKDPKFAEMEERFYRYGIKPEWMMIHRILNHSVDKKGHVHYLIKWRDLPYDQASWESEDVEIQDYDLFKQSYWNHRELMRGEEGRPGKKLKKVKLRKLERPPETPTVDPTVKYERQPEYLDATGGTLHPYQMEGLNWLRFSWAQGTDTILADEMGLGKTVQTAVFLYSLYKEGHSKGPFLVSAPLSTIINWEREFEMWAPDMYVVTYVGDKDSRAIIRENEFSFEDNAIRGGKKASRMKKEASVKFHVLLTSYELITIDMAILGSIDWACLIVDEAHRLKNNQSKFFRVLNGYSLQHKLLLTGTPLQNNLEELFHLLNFLTPERFHNLEGFLEEFADIAKEDQIKKLHDMLGPHMLRRLKADVFKNMPSKTELIVRVELSPMQKKYYKYILTRNFEALNARGGGNQVSLLNVVMDLKKCCNHPYLFPVAAMEAPKMPNGMYDGSALIRASGKLLLLQKMLKNLKEGGHRVLIFSQMTKMLDLLEDFLEHEGYKYERIDGGITGNMRQEAIDRFNAPGAQQFCFLLSTRAGGLGINLATADTVIIYDSDWNPHNDIQAFSRAHRIGQNKKVMIYRFVTRASVEERITQVAKKKMMLTHLVVRPGLGSKTGSMSKQELDDILKFGTEELFKDEATDGGGDNKEGEDSSVIHYDDKAIERLLDRNQDETEDTELQGMNEYLSSFKVAQYVVREEEMGEEEEVEREIIKQEESVDPDYWEKLLRHHYEQQQEDLARNLGKGKRIRKQVNYNDGSQEDRDWQDDQSDNQSDYSVASEEGDEDFDERSEAPRRPSRKGLRNDKDKPLPPLLARVGGNIEVLGFNARQRKAFLNAIMRYGMPPQDAFTTQWLVRDLRGKSEKEFKAYVSLFMRHLCEPGADGAETFADGVPREGLSRQHVLTRIGVMSLIRKKVQEFEHVNGRWSMPELAEVEENKKMSQPGSPSPKTPTPSTPGDTQPNTPAPVPPAEDGIKIEENSLKEEESIEGEKEVKSTAPETAIECTQAPAPASEDEKVVVEPPEGEEKVEKAEVKERTEEPMETEPKGAADVEKVEEKSAIDLTPIVVEDKEEKKEEEEKKEVMLQNGETPKDLNDEKQKKNIKQRFMFNIADGGFTELHSLWQNEERAATVTKKTYEIWHRRHDYWLLAGIINHGYARWQDIQNDPRYAILNEPFKGEMNRGNFLEIKNKFLARRFKLLEQALVIEEQLRRAAYLNMSEDPSHPSMALNTRFAEVECLAESHQHLSKESMAGNKPANAVLHKVLKQLEELLSDMKADVTRLPATIARIPPVAVRLQMSERNILSRLANRAPEPTPQQVAQQQ

Target ORF information:

RefSeq Version	XM_017018731.1
Organism	Homo sapiens(human)
Definition	Homo sapiens chromodomain helicase DNA binding protein 4 (CHD4), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017018731.1

ORF Insert Sequence:

ATGGCGTCGG GCCTGGGCTC CCCGTCCCCC TGCTCGGCGG GCAGTGAGGA GGAGGATATG 
GATGCACTTT TGAACAACAG CCTGCCCCCA CCCCACCCAG AAAATGAAGA GGACCCAGAA 
GAGGATTTGT CAGAAACAGA GACTCCAAAG CTCAAGAAGA AGAAAAAGCC TAAGAAACCT 
CGGGACCCTA AAATCCCTAA GAGCAAGCGC CAAAAAAAGG AGCGTATGCT CTTATGCCGG 
CAGCTGGGGG ACAGCTCTGG GGAGGGGCCA GAGTTTGTGG AGGAGGAGGA AGAGGTGGCT 
CTGCGCTCAG ACAGTGAGGG CAGCGACTAT ACTCCTGGCA AGAAGAAGAA GAAGAAGCTT 
GGACCTAAGA AAGAGAAGAA GAGCAAATCC AAGCGGAAGG AGGAGGAGGA GGAGGAGGAT 
GATGATGATG ATTCAAAGGA GCCTAAATCA TCTGCTCAGC TCCTGGAAGA CTGGGGCATG 
GAAGACATTG ACCACGTGTT CTCAGAGGAG GATTATCGAA CCCTCACCAA CTACAAGGCC 
TTCAGCCAGT TTGTCAGACC CCTCATTGCT GCCAAAAATC CCAAGATTGC TGTCTCCAAG 
ATGATGATGG TTTTGGGTGC AAAATGGCGG GAGTTCAGTA CCAATAACCC CTTCAAAGGC 
AGTTCTGGGG CATCAGTGGC AGCTGCGGCA GCAGCAGCGG TAGCTGTGGT GGAGAGCATG 
GTGACAGCCA CTGAGGTTGC ACCACCACCT CCCCCTGTGG AGGTGCCTAT CCGCAAGGCC 
AAGACCAAGG AGGGCAAAGG TCCCAATGCT CGGAGGAAGC CCAAGGGCAG CCCTCGTGTA 
CCTGATGCCA AGAAGCCTAA ACCCAAGAAA GTAGCTCCCC TGAAAATCAA GCTGGGAGGT 
TTTGGTTCCA AGCGTAAGAG ATCCTCGAGT GAGGATGATG ACTTAGATGT GGAATCTGAC 
TTCGATGATG CCAGTATCAA TAGCTATTCT GTTTCTGATG GTTCCACCAG CCGTAGTAGC 
CGCAGCCGCA AGAAACTCCG AACCACTAAA AAGAAAAAGA AAGACCACCA GGACTATTGC 
GAGGTGTGCC AGCAAGGCGG TGAGATCATC CTGTGTGATA CCTGTCCCCG TGCTTACCAC 
ATGGTCTGCC TGGATCCCGA CATGGAGAAG GCTCCCGAGG GCAAGTGGAG CTGCCCACAC 
TGCGAGAAGG AAGGCATCCA GTGGGAAGCT AAAGAGGACA ATTCGGAGGG TGAGGAGATC 
CTGGAAGAGG TTGGGGGAGA CCTCGAAGAG GAGGATGACC ACCATATGGA ATTCTGTCGG 
GTCTGCAAGG ATGGTGGGGA ACTGCTCTGC TGTGATACCT GTCCTTCTTC CTACCACATC 
CACTGCCTGA ATCCCCCACT TCCAGAGATC CCCAACGGTG AATGGCTCTG TCCCCGTTGT 
ACGTGTCCAG CTCTGAAGGG CAAAGTGCAG AAGATCCTAA TCTGGAAGTG GGGTCAGCCA 
CCATCTCCCA CACCAGTGCC TCGGCCTCCA GATGCTGATC CCAACACGCC CTCCCCAAAG 
CCCTTGGAGG GGCGGCCAGA GCGGCAGTTC TTTGTGAAAT GGCAAGGCAT GTCTTACTGG 
CACTGCTCCT GGGTTTCTGA ACTGCAGCTG GAGCTGCACT GTCAGGTGAT GTTCCGAAAC 
TATCAGCGGA AGAATGATAT GGATGAGCCA CCTTCTGGGG ACTTTGGTGG TGATGAAGAG 
AAAAGCCGAA AGCGAAAGAA CAAGGACCCT AAATTTGCAG AGATGGAGGA ACGCTTCTAT 
CGCTATGGGA TAAAACCCGA GTGGATGATG ATCCACCGAA TCCTCAACCA CAGTGTGGAC 
AAGAAGGGCC ACGTCCACTA CTTGATCAAG TGGCGGGACT TACCTTACGA TCAGGCTTCT 
TGGGAGAGTG AGGATGTGGA GATCCAGGAT TACGACCTGT TCAAGCAGAG CTATTGGAAT 
CACAGGGAGT TAATGAGGGG TGAGGAAGGC CGACCAGGCA AGAAGCTCAA GAAGGTGAAG 
CTTCGGAAGT TGGAGAGGCC TCCAGAAACG CCAACAGTTG ATCCAACAGT GAAGTATGAG 
CGACAGCCAG AGTACCTGGA TGCTACAGGT GGAACCCTGC ACCCCTATCA AATGGAGGGC 
CTGAATTGGT TGCGCTTCTC CTGGGCTCAG GGCACTGACA CCATCTTGGC TGATGAGATG 
GGCCTTGGGA AAACTGTACA GACAGCAGTC TTCCTGTATT CCCTTTACAA GGAGGGTCAT 
TCCAAAGGCC CCTTCCTAGT GAGCGCCCCT CTTTCTACCA TCATCAACTG GGAGCGGGAG 
TTTGAAATGT GGGCTCCAGA CATGTATGTC GTAACCTATG TGGGTGACAA GGACAGCCGT 
GCCATCATCC GAGAGAATGA GTTCTCCTTT GAAGACAATG CCATTCGTGG TGGCAAGAAG 
GCCTCCCGCA TGAAGAAAGA GGCATCTGTG AAATTCCATG TGCTGCTGAC ATCCTATGAA 
TTGATCACCA TTGACATGGC TATTTTGGGC TCTATTGATT GGGCCTGCCT CATCGTGGAT 
GAAGCCCATC GGCTGAAGAA CAATCAGTCT AAGTTCTTCC GGGTATTGAA TGGTTACTCA 
CTCCAGCACA AGCTGTTGCT GACTGGGACA CCATTACAAA ACAATCTGGA AGAGTTGTTT 
CATCTGCTCA ACTTTCTCAC CCCCGAGAGG TTCCACAATT TGGAAGGTTT TTTGGAGGAG 
TTTGCTGACA TTGCCAAGGA GGACCAGATA AAAAAACTGC ATGACATGCT GGGGCCGCAC 
ATGTTGCGGC GGCTCAAAGC CGATGTGTTC AAGAACATGC CCTCCAAGAC AGAACTAATT 
GTGCGTGTGG AGCTGAGCCC TATGCAGAAG AAATACTACA AGTACATCCT CACTCGAAAT 
TTTGAAGCAC TCAATGCCCG AGGTGGTGGC AACCAGGTGT CTCTGCTGAA TGTGGTGATG 
GATCTTAAGA AGTGCTGCAA CCATCCATAC CTCTTCCCTG TGGCTGCAAT GGAAGCTCCT 
AAGATGCCTA ATGGCATGTA TGATGGCAGT GCCCTAATCA GAGCATCTGG GAAATTATTG 
CTGCTGCAGA AAATGCTCAA GAACCTTAAG GAGGGTGGGC ATCGTGTACT CATCTTTTCC 
CAGATGACCA AGATGCTAGA CCTGCTAGAG GATTTCTTGG AACATGAAGG TTATAAATAC 
GAACGCATCG ATGGTGGAAT CACTGGGAAC ATGCGGCAAG AGGCCATTGA CCGCTTCAAT 
GCACCGGGTG CTCAGCAGTT CTGCTTCTTG CTTTCCACTC GAGCTGGGGG CCTTGGAATC 
AATCTGGCCA CTGCTGACAC AGTTATTATC TATGACTCTG ACTGGAACCC CCATAATGAC 
ATTCAGGCCT TTAGCAGAGC TCACCGGATT GGGCAAAATA AAAAGGTAAT GATCTACCGG 
TTTGTGACCC GTGCGTCAGT GGAGGAGCGC ATCACGCAGG TGGCAAAGAA GAAAATGATG 
CTGACGCATC TAGTGGTGCG GCCTGGGCTG GGCTCCAAGA CTGGATCTAT GTCCAAACAG 
GAGCTTGATG ATATCCTCAA ATTTGGCACT GAGGAACTAT TCAAGGATGA AGCCACTGAT 
GGAGGAGGAG ACAACAAAGA GGGAGAAGAT AGCAGTGTTA TCCACTACGA TGATAAGGCC 
ATTGAACGGC TGCTAGACCG TAACCAGGAT GAGACTGAAG ACACAGAATT GCAGGGCATG 
AATGAATATT TGAGCTCATT CAAAGTGGCC CAGTATGTGG TACGGGAAGA AGAAATGGGG 
GAGGAAGAGG AGGTAGAACG GGAAATCATT AAACAGGAAG AAAGTGTGGA TCCTGACTAC 
TGGGAGAAAT TGCTGCGGCA CCATTATGAG CAGCAGCAAG AAGATCTAGC CCGAAATCTG 
GGCAAAGGAA AAAGAATCCG TAAACAGGTC AACTACAATG ATGGCTCCCA GGAGGACCGA 
GATTGGCAGG ACGACCAGTC CGACAACCAG TCCGATTACT CAGTGGCTTC AGAGGAAGGT 
GATGAAGACT TTGATGAACG TTCAGAAGCT CCCCGTAGGC CCAGTCGTAA GGGCCTGCGG 
AATGATAAAG ATAAGCCATT GCCTCCTCTG TTGGCCCGTG TTGGTGGGAA TATTGAAGTA 
CTTGGTTTTA ATGCTCGTCA GCGAAAAGCC TTTCTTAATG CAATTATGCG ATATGGTATG 
CCACCTCAGG ATGCTTTTAC TACCCAGTGG CTTGTAAGAG ACCTGCGAGG CAAATCAGAG 
AAAGAGTTCA AGGCATATGT CTCTCTTTTC ATGCGGCATT TATGTGAGCC GGGGGCAGAT 
GGGGCTGAGA CCTTTGCTGA TGGTGTCCCC CGAGAAGGCC TGTCTCGCCA GCATGTCCTT 
ACTAGAATTG GTGTTATGTC TTTGATTCGC AAGAAGGTTC AGGAGTTTGA ACATGTTAAT 
GGGCGCTGGA GCATGCCTGA ACTGGCTGAG GTGGAGGAAA ACAAGAAGAT GTCCCAGCCA 
GGGTCACCCT CCCCAAAAAC TCCTACACCC TCCACTCCAG GGGACACGCA GCCCAACACT 
CCTGCACCTG TCCCACCTGC TGAAGATGGG ATAAAAATAG AGGAAAATAG CCTCAAAGAA 
GAAGAGAGCA TAGAAGGAGA AAAGGAGGTT AAATCTACAG CCCCTGAGAC TGCCATTGAG 
TGTACACAGG CCCCTGCCCC TGCCTCAGAG GATGAAAAGG TCGTTGTTGA ACCCCCTGAG 
GGAGAGGAGA AAGTGGAAAA GGCAGAGGTG AAGGAGAGAA CAGAGGAACC TATGGAGACA 
GAGCCCAAAG GTGCTGCTGA TGTAGAGAAG GTGGAGGAAA AGTCAGCAAT AGATCTGACC 
CCTATTGTGG TAGAAGACAA AGAAGAGAAG AAAGAAGAAG AAGAGAAAAA AGAGGTGATG 
CTTCAGAATG GAGAGACCCC CAAGGACCTG AATGATGAGA AACAGAAGAA AAATATTAAA 
CAACGTTTCA TGTTTAACAT TGCAGATGGT GGTTTTACTG AGTTGCACTC CCTTTGGCAG 
AATGAAGAGC GGGCAGCCAC AGTTACCAAG AAGACTTATG AGATCTGGCA TCGACGGCAT 
GACTACTGGC TGCTAGCCGG CATTATAAAC CATGGCTATG CCCGGTGGCA AGACATCCAG 
AATGACCCAC GCTATGCCAT CCTCAATGAG CCTTTCAAGG GTGAAATGAA CCGTGGCAAT 
TTCTTAGAGA TCAAGAATAA ATTTCTAGCT CGAAGGTTTA AGCTCTTAGA ACAAGCTCTG 
GTGATTGAGG AACAGCTGCG CCGGGCTGCT TACTTGAACA TGTCAGAAGA CCCTTCTCAC 
CCTTCCATGG CCCTCAACAC CCGCTTTGCT GAGGTGGAGT GTTTGGCGGA AAGTCATCAG 
CACCTGTCCA AGGAGTCAAT GGCAGGAAAC AAGCCAGCCA ATGCAGTCCT GCACAAAGTT 
CTGAAACAGC TGGAAGAACT GCTGAGTGAC ATGAAAGCTG ATGTGACTCG ACTCCCAGCT 
ACCATTGCCC GAATTCCCCC AGTTGCTGTG AGGTTACAGA TGTCAGAGCG TAACATTCTC 
AGCCGCCTGG CAAACCGGGC ACCCGAACCT ACCCCACAGC AGGTAGCCCA GCAGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHD4 ( XM_017018732.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016874221.1 Homo sapiens chromodomain helicase DNA binding protein 4 (CHD4), transcript variant X18, mRNA.

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CloneID	OHu83477
Clone ID Related Accession (Same CDS sequence)	XM_017018732.1
Accession Version	XM_017018732.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5688bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-25
Organism	Homo sapiens(human)
Product	chromodomain-helicase-DNA-binding protein 4 isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCGG GCCTGGGCTC CCCGTCCCCC TGCTCGGCGG GCAGTGAGGA GGAGGATATG 
GATGCACTTT TGAACAACAG CCTGCCCCCA CCCCACCCAG AAAATGAAGA GGACCCAGAA 
GAGGATTTGT CAGAAACAGA GACTCCAAAG CTCAAGAAGA AGAAAAAGCC TAAGAAACCT 
CGGGACCCTA AAATCCCTAA GAGCAAGCGC CAAAAAAAGG AGCTGGGGGA CAGCTCTGGG 
GAGGGGCCAG AGTTTGTGGA GGAGGAGGAA GAGGTGGCTC TGCGCTCAGA CAGTGAGGGC 
AGCGACTATA CTCCTGGCAA GAAGAAGAAG AAGAAGCTTG GACCTAAGAA AGAGAAGAAG 
AGCAAATCCA AGCGGAAGGA GGAGGAGGAG GAGGAGGATG ATGATGATGA TTCAAAGGAG 
CCTAAATCAT CTGCTCAGCT CCTGGAAGAC TGGGGCATGG AAGACATTGA CCACGTGTTC 
TCAGAGGAGG ATTATCGAAC CCTCACCAAC TACAAGGCCT TCAGCCAGTT TGTCAGACCC 
CTCATTGCTG CCAAAAATCC CAAGATTGCT GTCTCCAAGA TGATGATGGT TTTGGGTGCA 
AAATGGCGGG AGTTCAGTAC CAATAACCCC TTCAAAGGCA GTTCTGGGGC ATCAGTGGCA 
GCTGCGGCAG CAGCAGCGGT AGCTGTGGTG GAGAGCATGG TGACAGCCAC TGAGGTTGCA 
CCACCACCTC CCCCTGTGGA GGTGCCTATC CGCAAGGCCA AGACCAAGGA GGGCAAAGGT 
CCCAATGCTC GGAGGAAGCC CAAGGGCAGC CCTCGTGTAC CTGATGCCAA GAAGCCTAAA 
CCCAAGAAAG TAGCTCCCCT GAAAATCAAG CTGGGAGGTT TTGGTTCCAA GCGTAAGAGA 
TCCTCGAGTG AGGATGATGA CTTAGATGTG GAATCTGACT TCGATGATGC CAGTATCAAT 
AGCTATTCTG TTTCTGATGG TTCCACCAGC CGTAGTAGCC GCAGCCGCAA GAAACTCCGA 
ACCACTAAAA AGAAAAAGAA AGACCACCAG GACTATTGCG AGGTGTGCCA GCAAGGCGGT 
GAGATCATCC TGTGTGATAC CTGTCCCCGT GCTTACCACA TGGTCTGCCT GGATCCCGAC 
ATGGAGAAGG CTCCCGAGGG CAAGTGGAGC TGCCCACACT GCGAGAAGGA AGGCATCCAG 
TGGGAAGCTA AAGAGGACAA TTCGGAGGGT GAGGAGATCC TGGAAGAGGT TGGGGGAGAC 
CTCGAAGAGG AGGATGACCA CCATATGGAA TTCTGTCGGG TCTGCAAGGA TGGTGGGGAA 
CTGCTCTGCT GTGATACCTG TCCTTCTTCC TACCACATCC ACTGCCTGAA TCCCCCACTT 
CCAGAGATCC CCAACGGTGA ATGGCTCTGT CCCCGTTGTA CGTGTCCAGC TCTGAAGGGC 
AAAGTGCAGA AGATCCTAAT CTGGAAGTGG GGTCAGCCAC CATCTCCCAC ACCAGTGCCT 
CGGCCTCCAG ATGCTGATCC CAACACGCCC TCCCCAAAGC CCTTGGAGGG GCGGCCAGAG 
CGGCAGTTCT TTGTGAAATG GCAAGGCATG TCTTACTGGC ACTGCTCCTG GGTTTCTGAA 
CTGCAGCTGG AGCTGCACTG TCAGGTGATG TTCCGAAACT ATCAGCGGAA GAATGATATG 
GATGAGCCAC CTTCTGGGGA CTTTGGTGGT GATGAAGAGA AAAGCCGAAA GCGAAAGAAC 
AAGGACCCTA AATTTGCAGA GATGGAGGAA CGCTTCTATC GCTATGGGAT AAAACCCGAG 
TGGATGATGA TCCACCGAAT CCTCAACCAC AGTGTGGACA AGAAGGGCCA CGTCCACTAC 
TTGATCAAGT GGCGGGACTT ACCTTACGAT CAGGCTTCTT GGGAGAGTGA GGATGTGGAG 
ATCCAGGATT ACGACCTGTT CAAGCAGAGC TATTGGAATC ACAGGGAGTT AATGAGGGGT 
GAGGAAGGCC GACCAGGCAA GAAGCTCAAG AAGGTGAAGC TTCGGAAGTT GGAGAGGCCT 
CCAGAAACGC CAACAGTTGA TCCAACAGTG AAGTATGAGC GACAGCCAGA GTACCTGGAT 
GCTACAGGTG GAACCCTGCA CCCCTATCAA ATGGAGGGCC TGAATTGGTT GCGCTTCTCC 
TGGGCTCAGG GCACTGACAC CATCTTGGCT GATGAGATGG GCCTTGGGAA AACTGTACAG 
ACAGCAGTCT TCCTGTATTC CCTTTACAAG GAGGGTCATT CCAAAGGCCC CTTCCTAGTG 
AGCGCCCCTC TTTCTACCAT CATCAACTGG GAGCGGGAGT TTGAAATGTG GGCTCCAGAC 
ATGTATGTCG TAACCTATGT GGGTGACAAG GACAGCCGTG CCATCATCCG AGAGAATGAG 
TTCTCCTTTG AAGACAATGC CATTCGTGGT GGCAAGAAGG CCTCCCGCAT GAAGAAAGAG 
GCATCTGTGA AATTCCATGT GCTGCTGACA TCCTATGAAT TGATCACCAT TGACATGGCT 
ATTTTGGGCT CTATTGATTG GGCCTGCCTC ATCGTGGATG AAGCCCATCG GCTGAAGAAC 
AATCAGTCTA AGTTCTTCCG GGTATTGAAT GGTTACTCAC TCCAGCACAA GCTGTTGCTG 
ACTGGGACAC CATTACAAAA CAATCTGGAA GAGTTGTTTC ATCTGCTCAA CTTTCTCACC 
CCCGAGAGGT TCCACAATTT GGAAGGTTTT TTGGAGGAGT TTGCTGACAT TGCCAAGGAG 
GACCAGATAA AAAAACTGCA TGACATGCTG GGGCCGCACA TGTTGCGGCG GCTCAAAGCC 
GATGTGTTCA AGAACATGCC CTCCAAGACA GAACTAATTG TGCGTGTGGA GCTGAGCCCT 
ATGCAGAAGA AATACTACAA GTACATCCTC ACTCGAAATT TTGAAGCACT CAATGCCCGA 
GGTGGTGGCA ACCAGGTGTC TCTGCTGAAT GTGGTGATGG ATCTTAAGAA GTGCTGCAAC 
CATCCATACC TCTTCCCTGT GGCTGCAATG GAAGCTCCTA AGATGCCTAA TGGCATGTAT 
GATGGCAGTG CCCTAATCAG AGCATCTGGG AAATTATTGC TGCTGCAGAA AATGCTCAAG 
AACCTTAAGG AGGGTGGGCA TCGTGTACTC ATCTTTTCCC AGATGACCAA GATGCTAGAC 
CTGCTAGAGG ATTTCTTGGA ACATGAAGGT TATAAATACG AACGCATCGA TGGTGGAATC 
ACTGGGAACA TGCGGCAAGA GGCCATTGAC CGCTTCAATG CACCGGGTGC TCAGCAGTTC 
TGCTTCTTGC TTTCCACTCG AGCTGGGGGC CTTGGAATCA ATCTGGCCAC TGCTGACACA 
GTTATTATCT ATGACTCTGA CTGGAACCCC CATAATGACA TTCAGGCCTT TAGCAGAGCT 
CACCGGATTG GGCAAAATAA AAAGGTAATG ATCTACCGGT TTGTGACCCG TGCGTCAGTG 
GAGGAGCGCA TCACGCAGGT GGCAAAGAAG AAAATGATGC TGACGCATCT AGTGGTGCGG 
CCTGGGCTGG GCTCCAAGAC TGGATCTATG TCCAAACAGG AGCTTGATGA TATCCTCAAA 
TTTGGCACTG AGGAACTATT CAAGGATGAA GCCACTGATG GAGGAGGAGA CAACAAAGAG 
GGAGAAGATA GCAGTGTTAT CCACTACGAT GATAAGGCCA TTGAACGGCT GCTAGACCGT 
AACCAGGATG AGACTGAAGA CACAGAATTG CAGGGCATGA ATGAATATTT GAGCTCATTC 
AAAGTGGCCC AGTATGTGGT ACGGGAAGAA GAAATGGGGG AGGAAGAGGA GGTAGAACGG 
GAAATCATTA AACAGGAAGA AAGTGTGGAT CCTGACTACT GGGAGAAATT GCTGCGGCAC 
CATTATGAGC AGCAGCAAGA AGATCTAGCC CGAAATCTGG GCAAAGGAAA AAGAATCCGT 
AAACAGGTCA ACTACAATGA TGGCTCCCAG GAGGACCGAG ATTGGCAGGA CGACCAGTCC 
GACAACCAGT CCGATTACTC AGTGGCTTCA GAGGAAGGTG ATGAAGACTT TGATGAACGT 
TCAGAAGCTC CCCGTAGGCC CAGTCGTAAG GGCCTGCGGA ATGATAAAGA TAAGCCATTG 
CCTCCTCTGT TGGCCCGTGT TGGTGGGAAT ATTGAAGTAC TTGGTTTTAA TGCTCGTCAG 
CGAAAAGCCT TTCTTAATGC AATTATGCGA TATGGTATGC CACCTCAGGA TGCTTTTACT 
ACCCAGTGGC TTGTAAGAGA CCTGCGAGGC AAATCAGAGA AAGAGTTCAA GGCATATGTC 
TCTCTTTTCA TGCGGCATTT ATGTGAGCCG GGGGCAGATG GGGCTGAGAC CTTTGCTGAT 
GGTGTCCCCC GAGAAGGCCT GTCTCGCCAG CATGTCCTTA CTAGAATTGG TGTTATGTCT 
TTGATTCGCA AGAAGGTTCA GGAGTTTGAA CATGTTAATG GGCGCTGGAG CATGCCTGAA 
CTGGCTGAGG TGGAGGAAAA CAAGAAGATG TCCCAGCCAG GGTCACCCTC CCCAAAAACT 
CCTACACCCT CCACTCCAGG GGACACGCAG CCCAACACTC CTGCACCTGT CCCACCTGCT 
GAAGATGGGA TAAAAATAGA GGAAAATAGC CTCAAAGAAG AAGAGAGCAT AGAAGGAGAA 
AAGGAGGTTA AATCTACAGC CCCTGAGACT GCCATTGAGT GTACACAGGC CCCTGCCCCT 
GCCTCAGAGG ATGAAAAGGT CGTTGTTGAA CCCCCTGAGG GAGAGGAGAA AGTGGAAAAG 
GCAGAGGTGA AGGAGAGAAC AGAGGAACCT ATGGAGACAG AGCCCAAAGG TAAAGGTGCT 
GCTGATGTAG AGAAGGTGGA GGAAAAGTCA GCAATAGATC TGACCCCTAT TGTGGTAGAA 
GACAAAGAAG AGAAGAAAGA AGAAGAAGAG AAAAAAGAGG TGATGCTTCA GAATGGAGAG 
ACCCCCAAGG ACCTGAATGA TGAGAAACAG AAGAAAAATA TTAAACAACG TTTCATGTTT 
AACATTGCAG ATGGTGGTTT TACTGAGTTG CACTCCCTTT GGCAGAATGA AGAGCGGGCA 
GCCACAGTTA CCAAGAAGAC TTATGAGATC TGGCATCGAC GGCATGACTA CTGGCTGCTA 
GCCGGCATTA TAAACCATGG CTATGCCCGG TGGCAAGACA TCCAGAATGA CCCACGCTAT 
GCCATCCTCA ATGAGCCTTT CAAGGGTGAA ATGAACCGTG GCAATTTCTT AGAGATCAAG 
AATAAATTTC TAGCTCGAAG GTTTAAGCTC TTAGAACAAG CTCTGGTGAT TGAGGAACAG 
CTGCGCCGGG CTGCTTACTT GAACATGTCA GAAGACCCTT CTCACCCTTC CATGGCCCTC 
AACACCCGCT TTGCTGAGGT GGAGTGTTTG GCGGAAAGTC ATCAGCACCT GTCCAAGGAG 
TCAATGGCAG GAAACAAGCC AGCCAATGCA GTCCTGCACA AAGGTATTCT GAAACAGCTG 
GAAGAACTGC TGAGTGACAT GAAAGCTGAT GTGACTCGAC TCCCAGCTAC CATTGCCCGA 
ATTCCCCCAG TTGCTGTGAG GTTACAGATG TCAGAGCGTA ACATTCTCAG CCGCCTGGCA 
AACCGGGCAC CCGAACCTAC CCCACAGCAG GTAGCCCAGC AGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016874221.1
CDS	165..5852
Translation	MASGLGSPSPCSAGSEEEDMDALLNNSLPPPHPENEEDPEEDLSETETPKLKKKKKPKKPRDPKIPKSKRQKKELGDSSGEGPEFVEEEEEVALRSDSEGSDYTPGKKKKKKLGPKKEKKSKSKRKEEEEEEDDDDDSKEPKSSAQLLEDWGMEDIDHVFSEEDYRTLTNYKAFSQFVRPLIAAKNPKIAVSKMMMVLGAKWREFSTNNPFKGSSGASVAAAAAAAVAVVESMVTATEVAPPPPPVEVPIRKAKTKEGKGPNARRKPKGSPRVPDAKKPKPKKVAPLKIKLGGFGSKRKRSSSEDDDLDVESDFDDASINSYSVSDGSTSRSSRSRKKLRTTKKKKKDHQDYCEVCQQGGEIILCDTCPRAYHMVCLDPDMEKAPEGKWSCPHCEKEGIQWEAKEDNSEGEEILEEVGGDLEEEDDHHMEFCRVCKDGGELLCCDTCPSSYHIHCLNPPLPEIPNGEWLCPRCTCPALKGKVQKILIWKWGQPPSPTPVPRPPDADPNTPSPKPLEGRPERQFFVKWQGMSYWHCSWVSELQLELHCQVMFRNYQRKNDMDEPPSGDFGGDEEKSRKRKNKDPKFAEMEERFYRYGIKPEWMMIHRILNHSVDKKGHVHYLIKWRDLPYDQASWESEDVEIQDYDLFKQSYWNHRELMRGEEGRPGKKLKKVKLRKLERPPETPTVDPTVKYERQPEYLDATGGTLHPYQMEGLNWLRFSWAQGTDTILADEMGLGKTVQTAVFLYSLYKEGHSKGPFLVSAPLSTIINWEREFEMWAPDMYVVTYVGDKDSRAIIRENEFSFEDNAIRGGKKASRMKKEASVKFHVLLTSYELITIDMAILGSIDWACLIVDEAHRLKNNQSKFFRVLNGYSLQHKLLLTGTPLQNNLEELFHLLNFLTPERFHNLEGFLEEFADIAKEDQIKKLHDMLGPHMLRRLKADVFKNMPSKTELIVRVELSPMQKKYYKYILTRNFEALNARGGGNQVSLLNVVMDLKKCCNHPYLFPVAAMEAPKMPNGMYDGSALIRASGKLLLLQKMLKNLKEGGHRVLIFSQMTKMLDLLEDFLEHEGYKYERIDGGITGNMRQEAIDRFNAPGAQQFCFLLSTRAGGLGINLATADTVIIYDSDWNPHNDIQAFSRAHRIGQNKKVMIYRFVTRASVEERITQVAKKKMMLTHLVVRPGLGSKTGSMSKQELDDILKFGTEELFKDEATDGGGDNKEGEDSSVIHYDDKAIERLLDRNQDETEDTELQGMNEYLSSFKVAQYVVREEEMGEEEEVEREIIKQEESVDPDYWEKLLRHHYEQQQEDLARNLGKGKRIRKQVNYNDGSQEDRDWQDDQSDNQSDYSVASEEGDEDFDERSEAPRRPSRKGLRNDKDKPLPPLLARVGGNIEVLGFNARQRKAFLNAIMRYGMPPQDAFTTQWLVRDLRGKSEKEFKAYVSLFMRHLCEPGADGAETFADGVPREGLSRQHVLTRIGVMSLIRKKVQEFEHVNGRWSMPELAEVEENKKMSQPGSPSPKTPTPSTPGDTQPNTPAPVPPAEDGIKIEENSLKEEESIEGEKEVKSTAPETAIECTQAPAPASEDEKVVVEPPEGEEKVEKAEVKERTEEPMETEPKGKGAADVEKVEEKSAIDLTPIVVEDKEEKKEEEEKKEVMLQNGETPKDLNDEKQKKNIKQRFMFNIADGGFTELHSLWQNEERAATVTKKTYEIWHRRHDYWLLAGIINHGYARWQDIQNDPRYAILNEPFKGEMNRGNFLEIKNKFLARRFKLLEQALVIEEQLRRAAYLNMSEDPSHPSMALNTRFAEVECLAESHQHLSKESMAGNKPANAVLHKGILKQLEELLSDMKADVTRLPATIARIPPVAVRLQMSERNILSRLANRAPEPTPQQVAQQQ

Target ORF information:

RefSeq Version	XM_017018732.1
Organism	Homo sapiens(human)
Definition	Homo sapiens chromodomain helicase DNA binding protein 4 (CHD4), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017018732.1

ORF Insert Sequence:

ATGGCGTCGG GCCTGGGCTC CCCGTCCCCC TGCTCGGCGG GCAGTGAGGA GGAGGATATG 
GATGCACTTT TGAACAACAG CCTGCCCCCA CCCCACCCAG AAAATGAAGA GGACCCAGAA 
GAGGATTTGT CAGAAACAGA GACTCCAAAG CTCAAGAAGA AGAAAAAGCC TAAGAAACCT 
CGGGACCCTA AAATCCCTAA GAGCAAGCGC CAAAAAAAGG AGCTGGGGGA CAGCTCTGGG 
GAGGGGCCAG AGTTTGTGGA GGAGGAGGAA GAGGTGGCTC TGCGCTCAGA CAGTGAGGGC 
AGCGACTATA CTCCTGGCAA GAAGAAGAAG AAGAAGCTTG GACCTAAGAA AGAGAAGAAG 
AGCAAATCCA AGCGGAAGGA GGAGGAGGAG GAGGAGGATG ATGATGATGA TTCAAAGGAG 
CCTAAATCAT CTGCTCAGCT CCTGGAAGAC TGGGGCATGG AAGACATTGA CCACGTGTTC 
TCAGAGGAGG ATTATCGAAC CCTCACCAAC TACAAGGCCT TCAGCCAGTT TGTCAGACCC 
CTCATTGCTG CCAAAAATCC CAAGATTGCT GTCTCCAAGA TGATGATGGT TTTGGGTGCA 
AAATGGCGGG AGTTCAGTAC CAATAACCCC TTCAAAGGCA GTTCTGGGGC ATCAGTGGCA 
GCTGCGGCAG CAGCAGCGGT AGCTGTGGTG GAGAGCATGG TGACAGCCAC TGAGGTTGCA 
CCACCACCTC CCCCTGTGGA GGTGCCTATC CGCAAGGCCA AGACCAAGGA GGGCAAAGGT 
CCCAATGCTC GGAGGAAGCC CAAGGGCAGC CCTCGTGTAC CTGATGCCAA GAAGCCTAAA 
CCCAAGAAAG TAGCTCCCCT GAAAATCAAG CTGGGAGGTT TTGGTTCCAA GCGTAAGAGA 
TCCTCGAGTG AGGATGATGA CTTAGATGTG GAATCTGACT TCGATGATGC CAGTATCAAT 
AGCTATTCTG TTTCTGATGG TTCCACCAGC CGTAGTAGCC GCAGCCGCAA GAAACTCCGA 
ACCACTAAAA AGAAAAAGAA AGACCACCAG GACTATTGCG AGGTGTGCCA GCAAGGCGGT 
GAGATCATCC TGTGTGATAC CTGTCCCCGT GCTTACCACA TGGTCTGCCT GGATCCCGAC 
ATGGAGAAGG CTCCCGAGGG CAAGTGGAGC TGCCCACACT GCGAGAAGGA AGGCATCCAG 
TGGGAAGCTA AAGAGGACAA TTCGGAGGGT GAGGAGATCC TGGAAGAGGT TGGGGGAGAC 
CTCGAAGAGG AGGATGACCA CCATATGGAA TTCTGTCGGG TCTGCAAGGA TGGTGGGGAA 
CTGCTCTGCT GTGATACCTG TCCTTCTTCC TACCACATCC ACTGCCTGAA TCCCCCACTT 
CCAGAGATCC CCAACGGTGA ATGGCTCTGT CCCCGTTGTA CGTGTCCAGC TCTGAAGGGC 
AAAGTGCAGA AGATCCTAAT CTGGAAGTGG GGTCAGCCAC CATCTCCCAC ACCAGTGCCT 
CGGCCTCCAG ATGCTGATCC CAACACGCCC TCCCCAAAGC CCTTGGAGGG GCGGCCAGAG 
CGGCAGTTCT TTGTGAAATG GCAAGGCATG TCTTACTGGC ACTGCTCCTG GGTTTCTGAA 
CTGCAGCTGG AGCTGCACTG TCAGGTGATG TTCCGAAACT ATCAGCGGAA GAATGATATG 
GATGAGCCAC CTTCTGGGGA CTTTGGTGGT GATGAAGAGA AAAGCCGAAA GCGAAAGAAC 
AAGGACCCTA AATTTGCAGA GATGGAGGAA CGCTTCTATC GCTATGGGAT AAAACCCGAG 
TGGATGATGA TCCACCGAAT CCTCAACCAC AGTGTGGACA AGAAGGGCCA CGTCCACTAC 
TTGATCAAGT GGCGGGACTT ACCTTACGAT CAGGCTTCTT GGGAGAGTGA GGATGTGGAG 
ATCCAGGATT ACGACCTGTT CAAGCAGAGC TATTGGAATC ACAGGGAGTT AATGAGGGGT 
GAGGAAGGCC GACCAGGCAA GAAGCTCAAG AAGGTGAAGC TTCGGAAGTT GGAGAGGCCT 
CCAGAAACGC CAACAGTTGA TCCAACAGTG AAGTATGAGC GACAGCCAGA GTACCTGGAT 
GCTACAGGTG GAACCCTGCA CCCCTATCAA ATGGAGGGCC TGAATTGGTT GCGCTTCTCC 
TGGGCTCAGG GCACTGACAC CATCTTGGCT GATGAGATGG GCCTTGGGAA AACTGTACAG 
ACAGCAGTCT TCCTGTATTC CCTTTACAAG GAGGGTCATT CCAAAGGCCC CTTCCTAGTG 
AGCGCCCCTC TTTCTACCAT CATCAACTGG GAGCGGGAGT TTGAAATGTG GGCTCCAGAC 
ATGTATGTCG TAACCTATGT GGGTGACAAG GACAGCCGTG CCATCATCCG AGAGAATGAG 
TTCTCCTTTG AAGACAATGC CATTCGTGGT GGCAAGAAGG CCTCCCGCAT GAAGAAAGAG 
GCATCTGTGA AATTCCATGT GCTGCTGACA TCCTATGAAT TGATCACCAT TGACATGGCT 
ATTTTGGGCT CTATTGATTG GGCCTGCCTC ATCGTGGATG AAGCCCATCG GCTGAAGAAC 
AATCAGTCTA AGTTCTTCCG GGTATTGAAT GGTTACTCAC TCCAGCACAA GCTGTTGCTG 
ACTGGGACAC CATTACAAAA CAATCTGGAA GAGTTGTTTC ATCTGCTCAA CTTTCTCACC 
CCCGAGAGGT TCCACAATTT GGAAGGTTTT TTGGAGGAGT TTGCTGACAT TGCCAAGGAG 
GACCAGATAA AAAAACTGCA TGACATGCTG GGGCCGCACA TGTTGCGGCG GCTCAAAGCC 
GATGTGTTCA AGAACATGCC CTCCAAGACA GAACTAATTG TGCGTGTGGA GCTGAGCCCT 
ATGCAGAAGA AATACTACAA GTACATCCTC ACTCGAAATT TTGAAGCACT CAATGCCCGA 
GGTGGTGGCA ACCAGGTGTC TCTGCTGAAT GTGGTGATGG ATCTTAAGAA GTGCTGCAAC 
CATCCATACC TCTTCCCTGT GGCTGCAATG GAAGCTCCTA AGATGCCTAA TGGCATGTAT 
GATGGCAGTG CCCTAATCAG AGCATCTGGG AAATTATTGC TGCTGCAGAA AATGCTCAAG 
AACCTTAAGG AGGGTGGGCA TCGTGTACTC ATCTTTTCCC AGATGACCAA GATGCTAGAC 
CTGCTAGAGG ATTTCTTGGA ACATGAAGGT TATAAATACG AACGCATCGA TGGTGGAATC 
ACTGGGAACA TGCGGCAAGA GGCCATTGAC CGCTTCAATG CACCGGGTGC TCAGCAGTTC 
TGCTTCTTGC TTTCCACTCG AGCTGGGGGC CTTGGAATCA ATCTGGCCAC TGCTGACACA 
GTTATTATCT ATGACTCTGA CTGGAACCCC CATAATGACA TTCAGGCCTT TAGCAGAGCT 
CACCGGATTG GGCAAAATAA AAAGGTAATG ATCTACCGGT TTGTGACCCG TGCGTCAGTG 
GAGGAGCGCA TCACGCAGGT GGCAAAGAAG AAAATGATGC TGACGCATCT AGTGGTGCGG 
CCTGGGCTGG GCTCCAAGAC TGGATCTATG TCCAAACAGG AGCTTGATGA TATCCTCAAA 
TTTGGCACTG AGGAACTATT CAAGGATGAA GCCACTGATG GAGGAGGAGA CAACAAAGAG 
GGAGAAGATA GCAGTGTTAT CCACTACGAT GATAAGGCCA TTGAACGGCT GCTAGACCGT 
AACCAGGATG AGACTGAAGA CACAGAATTG CAGGGCATGA ATGAATATTT GAGCTCATTC 
AAAGTGGCCC AGTATGTGGT ACGGGAAGAA GAAATGGGGG AGGAAGAGGA GGTAGAACGG 
GAAATCATTA AACAGGAAGA AAGTGTGGAT CCTGACTACT GGGAGAAATT GCTGCGGCAC 
CATTATGAGC AGCAGCAAGA AGATCTAGCC CGAAATCTGG GCAAAGGAAA AAGAATCCGT 
AAACAGGTCA ACTACAATGA TGGCTCCCAG GAGGACCGAG ATTGGCAGGA CGACCAGTCC 
GACAACCAGT CCGATTACTC AGTGGCTTCA GAGGAAGGTG ATGAAGACTT TGATGAACGT 
TCAGAAGCTC CCCGTAGGCC CAGTCGTAAG GGCCTGCGGA ATGATAAAGA TAAGCCATTG 
CCTCCTCTGT TGGCCCGTGT TGGTGGGAAT ATTGAAGTAC TTGGTTTTAA TGCTCGTCAG 
CGAAAAGCCT TTCTTAATGC AATTATGCGA TATGGTATGC CACCTCAGGA TGCTTTTACT 
ACCCAGTGGC TTGTAAGAGA CCTGCGAGGC AAATCAGAGA AAGAGTTCAA GGCATATGTC 
TCTCTTTTCA TGCGGCATTT ATGTGAGCCG GGGGCAGATG GGGCTGAGAC CTTTGCTGAT 
GGTGTCCCCC GAGAAGGCCT GTCTCGCCAG CATGTCCTTA CTAGAATTGG TGTTATGTCT 
TTGATTCGCA AGAAGGTTCA GGAGTTTGAA CATGTTAATG GGCGCTGGAG CATGCCTGAA 
CTGGCTGAGG TGGAGGAAAA CAAGAAGATG TCCCAGCCAG GGTCACCCTC CCCAAAAACT 
CCTACACCCT CCACTCCAGG GGACACGCAG CCCAACACTC CTGCACCTGT CCCACCTGCT 
GAAGATGGGA TAAAAATAGA GGAAAATAGC CTCAAAGAAG AAGAGAGCAT AGAAGGAGAA 
AAGGAGGTTA AATCTACAGC CCCTGAGACT GCCATTGAGT GTACACAGGC CCCTGCCCCT 
GCCTCAGAGG ATGAAAAGGT CGTTGTTGAA CCCCCTGAGG GAGAGGAGAA AGTGGAAAAG 
GCAGAGGTGA AGGAGAGAAC AGAGGAACCT ATGGAGACAG AGCCCAAAGG TAAAGGTGCT 
GCTGATGTAG AGAAGGTGGA GGAAAAGTCA GCAATAGATC TGACCCCTAT TGTGGTAGAA 
GACAAAGAAG AGAAGAAAGA AGAAGAAGAG AAAAAAGAGG TGATGCTTCA GAATGGAGAG 
ACCCCCAAGG ACCTGAATGA TGAGAAACAG AAGAAAAATA TTAAACAACG TTTCATGTTT 
AACATTGCAG ATGGTGGTTT TACTGAGTTG CACTCCCTTT GGCAGAATGA AGAGCGGGCA 
GCCACAGTTA CCAAGAAGAC TTATGAGATC TGGCATCGAC GGCATGACTA CTGGCTGCTA 
GCCGGCATTA TAAACCATGG CTATGCCCGG TGGCAAGACA TCCAGAATGA CCCACGCTAT 
GCCATCCTCA ATGAGCCTTT CAAGGGTGAA ATGAACCGTG GCAATTTCTT AGAGATCAAG 
AATAAATTTC TAGCTCGAAG GTTTAAGCTC TTAGAACAAG CTCTGGTGAT TGAGGAACAG 
CTGCGCCGGG CTGCTTACTT GAACATGTCA GAAGACCCTT CTCACCCTTC CATGGCCCTC 
AACACCCGCT TTGCTGAGGT GGAGTGTTTG GCGGAAAGTC ATCAGCACCT GTCCAAGGAG 
TCAATGGCAG GAAACAAGCC AGCCAATGCA GTCCTGCACA AAGGTATTCT GAAACAGCTG 
GAAGAACTGC TGAGTGACAT GAAAGCTGAT GTGACTCGAC TCCCAGCTAC CATTGCCCGA 
ATTCCCCCAG TTGCTGTGAG GTTACAGATG TCAGAGCGTA ACATTCTCAG CCGCCTGGCA 
AACCGGGCAC CCGAACCTAC CCCACAGCAG GTAGCCCAGC AGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHD4 ( XM_017018733.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016874222.1 Homo sapiens chromodomain helicase DNA binding protein 4 (CHD4), transcript variant X19, mRNA.

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CloneID	OHu83478
Clone ID Related Accession (Same CDS sequence)	XM_017018733.1
Accession Version	XM_017018733.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5682bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-25
Organism	Homo sapiens(human)
Product	chromodomain-helicase-DNA-binding protein 4 isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCGG GCCTGGGCTC CCCGTCCCCC TGCTCGGCGG GCAGTGAGGA GGAGGATATG 
GATGCACTTT TGAACAACAG CCTGCCCCCA CCCCACCCAG AAAATGAAGA GGACCCAGAA 
GAGGATTTGT CAGAAACAGA GACTCCAAAG CTCAAGAAGA AGAAAAAGCC TAAGAAACCT 
CGGGACCCTA AAATCCCTAA GAGCAAGCGC CAAAAAAAGG AGCTGGGGGA CAGCTCTGGG 
GAGGGGCCAG AGTTTGTGGA GGAGGAGGAA GAGGTGGCTC TGCGCTCAGA CAGTGAGGGC 
AGCGACTATA CTCCTGGCAA GAAGAAGAAG AAGAAGCTTG GACCTAAGAA AGAGAAGAAG 
AGCAAATCCA AGCGGAAGGA GGAGGAGGAG GAGGAGGATG ATGATGATGA TTCAAAGGAG 
CCTAAATCAT CTGCTCAGCT CCTGGAAGAC TGGGGCATGG AAGACATTGA CCACGTGTTC 
TCAGAGGAGG ATTATCGAAC CCTCACCAAC TACAAGGCCT TCAGCCAGTT TGTCAGACCC 
CTCATTGCTG CCAAAAATCC CAAGATTGCT GTCTCCAAGA TGATGATGGT TTTGGGTGCA 
AAATGGCGGG AGTTCAGTAC CAATAACCCC TTCAAAGGCA GTTCTGGGGC ATCAGTGGCA 
GCTGCGGCAG CAGCAGCGGT AGCTGTGGTG GAGAGCATGG TGACAGCCAC TGAGGTTGCA 
CCACCACCTC CCCCTGTGGA GGTGCCTATC CGCAAGGCCA AGACCAAGGA GGGCAAAGGT 
CCCAATGCTC GGAGGAAGCC CAAGGGCAGC CCTCGTGTAC CTGATGCCAA GAAGCCTAAA 
CCCAAGAAAG TAGCTCCCCT GAAAATCAAG CTGGGAGGTT TTGGTTCCAA GCGTAAGAGA 
TCCTCGAGTG AGGATGATGA CTTAGATGTG GAATCTGACT TCGATGATGC CAGTATCAAT 
AGCTATTCTG TTTCTGATGG TTCCACCAGC CGTAGTAGCC GCAGCCGCAA GAAACTCCGA 
ACCACTAAAA AGAAAAAGAA AGACCACCAG GACTATTGCG AGGTGTGCCA GCAAGGCGGT 
GAGATCATCC TGTGTGATAC CTGTCCCCGT GCTTACCACA TGGTCTGCCT GGATCCCGAC 
ATGGAGAAGG CTCCCGAGGG CAAGTGGAGC TGCCCACACT GCGAGAAGGA AGGCATCCAG 
TGGGAAGCTA AAGAGGACAA TTCGGAGGGT GAGGAGATCC TGGAAGAGGT TGGGGGAGAC 
CTCGAAGAGG AGGATGACCA CCATATGGAA TTCTGTCGGG TCTGCAAGGA TGGTGGGGAA 
CTGCTCTGCT GTGATACCTG TCCTTCTTCC TACCACATCC ACTGCCTGAA TCCCCCACTT 
CCAGAGATCC CCAACGGTGA ATGGCTCTGT CCCCGTTGTA CGTGTCCAGC TCTGAAGGGC 
AAAGTGCAGA AGATCCTAAT CTGGAAGTGG GGTCAGCCAC CATCTCCCAC ACCAGTGCCT 
CGGCCTCCAG ATGCTGATCC CAACACGCCC TCCCCAAAGC CCTTGGAGGG GCGGCCAGAG 
CGGCAGTTCT TTGTGAAATG GCAAGGCATG TCTTACTGGC ACTGCTCCTG GGTTTCTGAA 
CTGCAGCTGG AGCTGCACTG TCAGGTGATG TTCCGAAACT ATCAGCGGAA GAATGATATG 
GATGAGCCAC CTTCTGGGGA CTTTGGTGGT GATGAAGAGA AAAGCCGAAA GCGAAAGAAC 
AAGGACCCTA AATTTGCAGA GATGGAGGAA CGCTTCTATC GCTATGGGAT AAAACCCGAG 
TGGATGATGA TCCACCGAAT CCTCAACCAC AGTGTGGACA AGAAGGGCCA CGTCCACTAC 
TTGATCAAGT GGCGGGACTT ACCTTACGAT CAGGCTTCTT GGGAGAGTGA GGATGTGGAG 
ATCCAGGATT ACGACCTGTT CAAGCAGAGC TATTGGAATC ACAGGGAGTT AATGAGGGGT 
GAGGAAGGCC GACCAGGCAA GAAGCTCAAG AAGGTGAAGC TTCGGAAGTT GGAGAGGCCT 
CCAGAAACGC CAACAGTTGA TCCAACAGTG AAGTATGAGC GACAGCCAGA GTACCTGGAT 
GCTACAGGTG GAACCCTGCA CCCCTATCAA ATGGAGGGCC TGAATTGGTT GCGCTTCTCC 
TGGGCTCAGG GCACTGACAC CATCTTGGCT GATGAGATGG GCCTTGGGAA AACTGTACAG 
ACAGCAGTCT TCCTGTATTC CCTTTACAAG GAGGGTCATT CCAAAGGCCC CTTCCTAGTG 
AGCGCCCCTC TTTCTACCAT CATCAACTGG GAGCGGGAGT TTGAAATGTG GGCTCCAGAC 
ATGTATGTCG TAACCTATGT GGGTGACAAG GACAGCCGTG CCATCATCCG AGAGAATGAG 
TTCTCCTTTG AAGACAATGC CATTCGTGGT GGCAAGAAGG CCTCCCGCAT GAAGAAAGAG 
GCATCTGTGA AATTCCATGT GCTGCTGACA TCCTATGAAT TGATCACCAT TGACATGGCT 
ATTTTGGGCT CTATTGATTG GGCCTGCCTC ATCGTGGATG AAGCCCATCG GCTGAAGAAC 
AATCAGTCTA AGTTCTTCCG GGTATTGAAT GGTTACTCAC TCCAGCACAA GCTGTTGCTG 
ACTGGGACAC CATTACAAAA CAATCTGGAA GAGTTGTTTC ATCTGCTCAA CTTTCTCACC 
CCCGAGAGGT TCCACAATTT GGAAGGTTTT TTGGAGGAGT TTGCTGACAT TGCCAAGGAG 
GACCAGATAA AAAAACTGCA TGACATGCTG GGGCCGCACA TGTTGCGGCG GCTCAAAGCC 
GATGTGTTCA AGAACATGCC CTCCAAGACA GAACTAATTG TGCGTGTGGA GCTGAGCCCT 
ATGCAGAAGA AATACTACAA GTACATCCTC ACTCGAAATT TTGAAGCACT CAATGCCCGA 
GGTGGTGGCA ACCAGGTGTC TCTGCTGAAT GTGGTGATGG ATCTTAAGAA GTGCTGCAAC 
CATCCATACC TCTTCCCTGT GGCTGCAATG GAAGCTCCTA AGATGCCTAA TGGCATGTAT 
GATGGCAGTG CCCTAATCAG AGCATCTGGG AAATTATTGC TGCTGCAGAA AATGCTCAAG 
AACCTTAAGG AGGGTGGGCA TCGTGTACTC ATCTTTTCCC AGATGACCAA GATGCTAGAC 
CTGCTAGAGG ATTTCTTGGA ACATGAAGGT TATAAATACG AACGCATCGA TGGTGGAATC 
ACTGGGAACA TGCGGCAAGA GGCCATTGAC CGCTTCAATG CACCGGGTGC TCAGCAGTTC 
TGCTTCTTGC TTTCCACTCG AGCTGGGGGC CTTGGAATCA ATCTGGCCAC TGCTGACACA 
GTTATTATCT ATGACTCTGA CTGGAACCCC CATAATGACA TTCAGGCCTT TAGCAGAGCT 
CACCGGATTG GGCAAAATAA AAAGGTAATG ATCTACCGGT TTGTGACCCG TGCGTCAGTG 
GAGGAGCGCA TCACGCAGGT GGCAAAGAAG AAAATGATGC TGACGCATCT AGTGGTGCGG 
CCTGGGCTGG GCTCCAAGAC TGGATCTATG TCCAAACAGG AGCTTGATGA TATCCTCAAA 
TTTGGCACTG AGGAACTATT CAAGGATGAA GCCACTGATG GAGGAGGAGA CAACAAAGAG 
GGAGAAGATA GCAGTGTTAT CCACTACGAT GATAAGGCCA TTGAACGGCT GCTAGACCGT 
AACCAGGATG AGACTGAAGA CACAGAATTG CAGGGCATGA ATGAATATTT GAGCTCATTC 
AAAGTGGCCC AGTATGTGGT ACGGGAAGAA GAAATGGGGG AGGAAGAGGA GGTAGAACGG 
GAAATCATTA AACAGGAAGA AAGTGTGGAT CCTGACTACT GGGAGAAATT GCTGCGGCAC 
CATTATGAGC AGCAGCAAGA AGATCTAGCC CGAAATCTGG GCAAAGGAAA AAGAATCCGT 
AAACAGGTCA ACTACAATGA TGGCTCCCAG GAGGACCGAG ATTGGCAGGA CGACCAGTCC 
GACAACCAGT CCGATTACTC AGTGGCTTCA GAGGAAGGTG ATGAAGACTT TGATGAACGT 
TCAGAAGCTC CCCGTAGGCC CAGTCGTAAG GGCCTGCGGA ATGATAAAGA TAAGCCATTG 
CCTCCTCTGT TGGCCCGTGT TGGTGGGAAT ATTGAAGTAC TTGGTTTTAA TGCTCGTCAG 
CGAAAAGCCT TTCTTAATGC AATTATGCGA TATGGTATGC CACCTCAGGA TGCTTTTACT 
ACCCAGTGGC TTGTAAGAGA CCTGCGAGGC AAATCAGAGA AAGAGTTCAA GGCATATGTC 
TCTCTTTTCA TGCGGCATTT ATGTGAGCCG GGGGCAGATG GGGCTGAGAC CTTTGCTGAT 
GGTGTCCCCC GAGAAGGCCT GTCTCGCCAG CATGTCCTTA CTAGAATTGG TGTTATGTCT 
TTGATTCGCA AGAAGGTTCA GGAGTTTGAA CATGTTAATG GGCGCTGGAG CATGCCTGAA 
CTGGCTGAGG TGGAGGAAAA CAAGAAGATG TCCCAGCCAG GGTCACCCTC CCCAAAAACT 
CCTACACCCT CCACTCCAGG GGACACGCAG CCCAACACTC CTGCACCTGT CCCACCTGCT 
GAAGATGGGA TAAAAATAGA GGAAAATAGC CTCAAAGAAG AAGAGAGCAT AGAAGGAGAA 
AAGGAGGTTA AATCTACAGC CCCTGAGACT GCCATTGAGT GTACACAGGC CCCTGCCCCT 
GCCTCAGAGG ATGAAAAGGT CGTTGTTGAA CCCCCTGAGG GAGAGGAGAA AGTGGAAAAG 
GCAGAGGTGA AGGAGAGAAC AGAGGAACCT ATGGAGACAG AGCCCAAAGG TGCTGCTGAT 
GTAGAGAAGG TGGAGGAAAA GTCAGCAATA GATCTGACCC CTATTGTGGT AGAAGACAAA 
GAAGAGAAGA AAGAAGAAGA AGAGAAAAAA GAGGTGATGC TTCAGAATGG AGAGACCCCC 
AAGGACCTGA ATGATGAGAA ACAGAAGAAA AATATTAAAC AACGTTTCAT GTTTAACATT 
GCAGATGGTG GTTTTACTGA GTTGCACTCC CTTTGGCAGA ATGAAGAGCG GGCAGCCACA 
GTTACCAAGA AGACTTATGA GATCTGGCAT CGACGGCATG ACTACTGGCT GCTAGCCGGC 
ATTATAAACC ATGGCTATGC CCGGTGGCAA GACATCCAGA ATGACCCACG CTATGCCATC 
CTCAATGAGC CTTTCAAGGG TGAAATGAAC CGTGGCAATT TCTTAGAGAT CAAGAATAAA 
TTTCTAGCTC GAAGGTTTAA GCTCTTAGAA CAAGCTCTGG TGATTGAGGA ACAGCTGCGC 
CGGGCTGCTT ACTTGAACAT GTCAGAAGAC CCTTCTCACC CTTCCATGGC CCTCAACACC 
CGCTTTGCTG AGGTGGAGTG TTTGGCGGAA AGTCATCAGC ACCTGTCCAA GGAGTCAATG 
GCAGGAAACA AGCCAGCCAA TGCAGTCCTG CACAAAGGTA TTCTGAAACA GCTGGAAGAA 
CTGCTGAGTG ACATGAAAGC TGATGTGACT CGACTCCCAG CTACCATTGC CCGAATTCCC 
CCAGTTGCTG TGAGGTTACA GATGTCAGAG CGTAACATTC TCAGCCGCCT GGCAAACCGG 
GCACCCGAAC CTACCCCACA GCAGGTAGCC CAGCAGCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016874222.1
CDS	165..5846
Translation	MASGLGSPSPCSAGSEEEDMDALLNNSLPPPHPENEEDPEEDLSETETPKLKKKKKPKKPRDPKIPKSKRQKKELGDSSGEGPEFVEEEEEVALRSDSEGSDYTPGKKKKKKLGPKKEKKSKSKRKEEEEEEDDDDDSKEPKSSAQLLEDWGMEDIDHVFSEEDYRTLTNYKAFSQFVRPLIAAKNPKIAVSKMMMVLGAKWREFSTNNPFKGSSGASVAAAAAAAVAVVESMVTATEVAPPPPPVEVPIRKAKTKEGKGPNARRKPKGSPRVPDAKKPKPKKVAPLKIKLGGFGSKRKRSSSEDDDLDVESDFDDASINSYSVSDGSTSRSSRSRKKLRTTKKKKKDHQDYCEVCQQGGEIILCDTCPRAYHMVCLDPDMEKAPEGKWSCPHCEKEGIQWEAKEDNSEGEEILEEVGGDLEEEDDHHMEFCRVCKDGGELLCCDTCPSSYHIHCLNPPLPEIPNGEWLCPRCTCPALKGKVQKILIWKWGQPPSPTPVPRPPDADPNTPSPKPLEGRPERQFFVKWQGMSYWHCSWVSELQLELHCQVMFRNYQRKNDMDEPPSGDFGGDEEKSRKRKNKDPKFAEMEERFYRYGIKPEWMMIHRILNHSVDKKGHVHYLIKWRDLPYDQASWESEDVEIQDYDLFKQSYWNHRELMRGEEGRPGKKLKKVKLRKLERPPETPTVDPTVKYERQPEYLDATGGTLHPYQMEGLNWLRFSWAQGTDTILADEMGLGKTVQTAVFLYSLYKEGHSKGPFLVSAPLSTIINWEREFEMWAPDMYVVTYVGDKDSRAIIRENEFSFEDNAIRGGKKASRMKKEASVKFHVLLTSYELITIDMAILGSIDWACLIVDEAHRLKNNQSKFFRVLNGYSLQHKLLLTGTPLQNNLEELFHLLNFLTPERFHNLEGFLEEFADIAKEDQIKKLHDMLGPHMLRRLKADVFKNMPSKTELIVRVELSPMQKKYYKYILTRNFEALNARGGGNQVSLLNVVMDLKKCCNHPYLFPVAAMEAPKMPNGMYDGSALIRASGKLLLLQKMLKNLKEGGHRVLIFSQMTKMLDLLEDFLEHEGYKYERIDGGITGNMRQEAIDRFNAPGAQQFCFLLSTRAGGLGINLATADTVIIYDSDWNPHNDIQAFSRAHRIGQNKKVMIYRFVTRASVEERITQVAKKKMMLTHLVVRPGLGSKTGSMSKQELDDILKFGTEELFKDEATDGGGDNKEGEDSSVIHYDDKAIERLLDRNQDETEDTELQGMNEYLSSFKVAQYVVREEEMGEEEEVEREIIKQEESVDPDYWEKLLRHHYEQQQEDLARNLGKGKRIRKQVNYNDGSQEDRDWQDDQSDNQSDYSVASEEGDEDFDERSEAPRRPSRKGLRNDKDKPLPPLLARVGGNIEVLGFNARQRKAFLNAIMRYGMPPQDAFTTQWLVRDLRGKSEKEFKAYVSLFMRHLCEPGADGAETFADGVPREGLSRQHVLTRIGVMSLIRKKVQEFEHVNGRWSMPELAEVEENKKMSQPGSPSPKTPTPSTPGDTQPNTPAPVPPAEDGIKIEENSLKEEESIEGEKEVKSTAPETAIECTQAPAPASEDEKVVVEPPEGEEKVEKAEVKERTEEPMETEPKGAADVEKVEEKSAIDLTPIVVEDKEEKKEEEEKKEVMLQNGETPKDLNDEKQKKNIKQRFMFNIADGGFTELHSLWQNEERAATVTKKTYEIWHRRHDYWLLAGIINHGYARWQDIQNDPRYAILNEPFKGEMNRGNFLEIKNKFLARRFKLLEQALVIEEQLRRAAYLNMSEDPSHPSMALNTRFAEVECLAESHQHLSKESMAGNKPANAVLHKGILKQLEELLSDMKADVTRLPATIARIPPVAVRLQMSERNILSRLANRAPEPTPQQVAQQQ

Target ORF information:

RefSeq Version	XM_017018733.1
Organism	Homo sapiens(human)
Definition	Homo sapiens chromodomain helicase DNA binding protein 4 (CHD4), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017018733.1

ORF Insert Sequence:

ATGGCGTCGG GCCTGGGCTC CCCGTCCCCC TGCTCGGCGG GCAGTGAGGA GGAGGATATG 
GATGCACTTT TGAACAACAG CCTGCCCCCA CCCCACCCAG AAAATGAAGA GGACCCAGAA 
GAGGATTTGT CAGAAACAGA GACTCCAAAG CTCAAGAAGA AGAAAAAGCC TAAGAAACCT 
CGGGACCCTA AAATCCCTAA GAGCAAGCGC CAAAAAAAGG AGCTGGGGGA CAGCTCTGGG 
GAGGGGCCAG AGTTTGTGGA GGAGGAGGAA GAGGTGGCTC TGCGCTCAGA CAGTGAGGGC 
AGCGACTATA CTCCTGGCAA GAAGAAGAAG AAGAAGCTTG GACCTAAGAA AGAGAAGAAG 
AGCAAATCCA AGCGGAAGGA GGAGGAGGAG GAGGAGGATG ATGATGATGA TTCAAAGGAG 
CCTAAATCAT CTGCTCAGCT CCTGGAAGAC TGGGGCATGG AAGACATTGA CCACGTGTTC 
TCAGAGGAGG ATTATCGAAC CCTCACCAAC TACAAGGCCT TCAGCCAGTT TGTCAGACCC 
CTCATTGCTG CCAAAAATCC CAAGATTGCT GTCTCCAAGA TGATGATGGT TTTGGGTGCA 
AAATGGCGGG AGTTCAGTAC CAATAACCCC TTCAAAGGCA GTTCTGGGGC ATCAGTGGCA 
GCTGCGGCAG CAGCAGCGGT AGCTGTGGTG GAGAGCATGG TGACAGCCAC TGAGGTTGCA 
CCACCACCTC CCCCTGTGGA GGTGCCTATC CGCAAGGCCA AGACCAAGGA GGGCAAAGGT 
CCCAATGCTC GGAGGAAGCC CAAGGGCAGC CCTCGTGTAC CTGATGCCAA GAAGCCTAAA 
CCCAAGAAAG TAGCTCCCCT GAAAATCAAG CTGGGAGGTT TTGGTTCCAA GCGTAAGAGA 
TCCTCGAGTG AGGATGATGA CTTAGATGTG GAATCTGACT TCGATGATGC CAGTATCAAT 
AGCTATTCTG TTTCTGATGG TTCCACCAGC CGTAGTAGCC GCAGCCGCAA GAAACTCCGA 
ACCACTAAAA AGAAAAAGAA AGACCACCAG GACTATTGCG AGGTGTGCCA GCAAGGCGGT 
GAGATCATCC TGTGTGATAC CTGTCCCCGT GCTTACCACA TGGTCTGCCT GGATCCCGAC 
ATGGAGAAGG CTCCCGAGGG CAAGTGGAGC TGCCCACACT GCGAGAAGGA AGGCATCCAG 
TGGGAAGCTA AAGAGGACAA TTCGGAGGGT GAGGAGATCC TGGAAGAGGT TGGGGGAGAC 
CTCGAAGAGG AGGATGACCA CCATATGGAA TTCTGTCGGG TCTGCAAGGA TGGTGGGGAA 
CTGCTCTGCT GTGATACCTG TCCTTCTTCC TACCACATCC ACTGCCTGAA TCCCCCACTT 
CCAGAGATCC CCAACGGTGA ATGGCTCTGT CCCCGTTGTA CGTGTCCAGC TCTGAAGGGC 
AAAGTGCAGA AGATCCTAAT CTGGAAGTGG GGTCAGCCAC CATCTCCCAC ACCAGTGCCT 
CGGCCTCCAG ATGCTGATCC CAACACGCCC TCCCCAAAGC CCTTGGAGGG GCGGCCAGAG 
CGGCAGTTCT TTGTGAAATG GCAAGGCATG TCTTACTGGC ACTGCTCCTG GGTTTCTGAA 
CTGCAGCTGG AGCTGCACTG TCAGGTGATG TTCCGAAACT ATCAGCGGAA GAATGATATG 
GATGAGCCAC CTTCTGGGGA CTTTGGTGGT GATGAAGAGA AAAGCCGAAA GCGAAAGAAC 
AAGGACCCTA AATTTGCAGA GATGGAGGAA CGCTTCTATC GCTATGGGAT AAAACCCGAG 
TGGATGATGA TCCACCGAAT CCTCAACCAC AGTGTGGACA AGAAGGGCCA CGTCCACTAC 
TTGATCAAGT GGCGGGACTT ACCTTACGAT CAGGCTTCTT GGGAGAGTGA GGATGTGGAG 
ATCCAGGATT ACGACCTGTT CAAGCAGAGC TATTGGAATC ACAGGGAGTT AATGAGGGGT 
GAGGAAGGCC GACCAGGCAA GAAGCTCAAG AAGGTGAAGC TTCGGAAGTT GGAGAGGCCT 
CCAGAAACGC CAACAGTTGA TCCAACAGTG AAGTATGAGC GACAGCCAGA GTACCTGGAT 
GCTACAGGTG GAACCCTGCA CCCCTATCAA ATGGAGGGCC TGAATTGGTT GCGCTTCTCC 
TGGGCTCAGG GCACTGACAC CATCTTGGCT GATGAGATGG GCCTTGGGAA AACTGTACAG 
ACAGCAGTCT TCCTGTATTC CCTTTACAAG GAGGGTCATT CCAAAGGCCC CTTCCTAGTG 
AGCGCCCCTC TTTCTACCAT CATCAACTGG GAGCGGGAGT TTGAAATGTG GGCTCCAGAC 
ATGTATGTCG TAACCTATGT GGGTGACAAG GACAGCCGTG CCATCATCCG AGAGAATGAG 
TTCTCCTTTG AAGACAATGC CATTCGTGGT GGCAAGAAGG CCTCCCGCAT GAAGAAAGAG 
GCATCTGTGA AATTCCATGT GCTGCTGACA TCCTATGAAT TGATCACCAT TGACATGGCT 
ATTTTGGGCT CTATTGATTG GGCCTGCCTC ATCGTGGATG AAGCCCATCG GCTGAAGAAC 
AATCAGTCTA AGTTCTTCCG GGTATTGAAT GGTTACTCAC TCCAGCACAA GCTGTTGCTG 
ACTGGGACAC CATTACAAAA CAATCTGGAA GAGTTGTTTC ATCTGCTCAA CTTTCTCACC 
CCCGAGAGGT TCCACAATTT GGAAGGTTTT TTGGAGGAGT TTGCTGACAT TGCCAAGGAG 
GACCAGATAA AAAAACTGCA TGACATGCTG GGGCCGCACA TGTTGCGGCG GCTCAAAGCC 
GATGTGTTCA AGAACATGCC CTCCAAGACA GAACTAATTG TGCGTGTGGA GCTGAGCCCT 
ATGCAGAAGA AATACTACAA GTACATCCTC ACTCGAAATT TTGAAGCACT CAATGCCCGA 
GGTGGTGGCA ACCAGGTGTC TCTGCTGAAT GTGGTGATGG ATCTTAAGAA GTGCTGCAAC 
CATCCATACC TCTTCCCTGT GGCTGCAATG GAAGCTCCTA AGATGCCTAA TGGCATGTAT 
GATGGCAGTG CCCTAATCAG AGCATCTGGG AAATTATTGC TGCTGCAGAA AATGCTCAAG 
AACCTTAAGG AGGGTGGGCA TCGTGTACTC ATCTTTTCCC AGATGACCAA GATGCTAGAC 
CTGCTAGAGG ATTTCTTGGA ACATGAAGGT TATAAATACG AACGCATCGA TGGTGGAATC 
ACTGGGAACA TGCGGCAAGA GGCCATTGAC CGCTTCAATG CACCGGGTGC TCAGCAGTTC 
TGCTTCTTGC TTTCCACTCG AGCTGGGGGC CTTGGAATCA ATCTGGCCAC TGCTGACACA 
GTTATTATCT ATGACTCTGA CTGGAACCCC CATAATGACA TTCAGGCCTT TAGCAGAGCT 
CACCGGATTG GGCAAAATAA AAAGGTAATG ATCTACCGGT TTGTGACCCG TGCGTCAGTG 
GAGGAGCGCA TCACGCAGGT GGCAAAGAAG AAAATGATGC TGACGCATCT AGTGGTGCGG 
CCTGGGCTGG GCTCCAAGAC TGGATCTATG TCCAAACAGG AGCTTGATGA TATCCTCAAA 
TTTGGCACTG AGGAACTATT CAAGGATGAA GCCACTGATG GAGGAGGAGA CAACAAAGAG 
GGAGAAGATA GCAGTGTTAT CCACTACGAT GATAAGGCCA TTGAACGGCT GCTAGACCGT 
AACCAGGATG AGACTGAAGA CACAGAATTG CAGGGCATGA ATGAATATTT GAGCTCATTC 
AAAGTGGCCC AGTATGTGGT ACGGGAAGAA GAAATGGGGG AGGAAGAGGA GGTAGAACGG 
GAAATCATTA AACAGGAAGA AAGTGTGGAT CCTGACTACT GGGAGAAATT GCTGCGGCAC 
CATTATGAGC AGCAGCAAGA AGATCTAGCC CGAAATCTGG GCAAAGGAAA AAGAATCCGT 
AAACAGGTCA ACTACAATGA TGGCTCCCAG GAGGACCGAG ATTGGCAGGA CGACCAGTCC 
GACAACCAGT CCGATTACTC AGTGGCTTCA GAGGAAGGTG ATGAAGACTT TGATGAACGT 
TCAGAAGCTC CCCGTAGGCC CAGTCGTAAG GGCCTGCGGA ATGATAAAGA TAAGCCATTG 
CCTCCTCTGT TGGCCCGTGT TGGTGGGAAT ATTGAAGTAC TTGGTTTTAA TGCTCGTCAG 
CGAAAAGCCT TTCTTAATGC AATTATGCGA TATGGTATGC CACCTCAGGA TGCTTTTACT 
ACCCAGTGGC TTGTAAGAGA CCTGCGAGGC AAATCAGAGA AAGAGTTCAA GGCATATGTC 
TCTCTTTTCA TGCGGCATTT ATGTGAGCCG GGGGCAGATG GGGCTGAGAC CTTTGCTGAT 
GGTGTCCCCC GAGAAGGCCT GTCTCGCCAG CATGTCCTTA CTAGAATTGG TGTTATGTCT 
TTGATTCGCA AGAAGGTTCA GGAGTTTGAA CATGTTAATG GGCGCTGGAG CATGCCTGAA 
CTGGCTGAGG TGGAGGAAAA CAAGAAGATG TCCCAGCCAG GGTCACCCTC CCCAAAAACT 
CCTACACCCT CCACTCCAGG GGACACGCAG CCCAACACTC CTGCACCTGT CCCACCTGCT 
GAAGATGGGA TAAAAATAGA GGAAAATAGC CTCAAAGAAG AAGAGAGCAT AGAAGGAGAA 
AAGGAGGTTA AATCTACAGC CCCTGAGACT GCCATTGAGT GTACACAGGC CCCTGCCCCT 
GCCTCAGAGG ATGAAAAGGT CGTTGTTGAA CCCCCTGAGG GAGAGGAGAA AGTGGAAAAG 
GCAGAGGTGA AGGAGAGAAC AGAGGAACCT ATGGAGACAG AGCCCAAAGG TGCTGCTGAT 
GTAGAGAAGG TGGAGGAAAA GTCAGCAATA GATCTGACCC CTATTGTGGT AGAAGACAAA 
GAAGAGAAGA AAGAAGAAGA AGAGAAAAAA GAGGTGATGC TTCAGAATGG AGAGACCCCC 
AAGGACCTGA ATGATGAGAA ACAGAAGAAA AATATTAAAC AACGTTTCAT GTTTAACATT 
GCAGATGGTG GTTTTACTGA GTTGCACTCC CTTTGGCAGA ATGAAGAGCG GGCAGCCACA 
GTTACCAAGA AGACTTATGA GATCTGGCAT CGACGGCATG ACTACTGGCT GCTAGCCGGC 
ATTATAAACC ATGGCTATGC CCGGTGGCAA GACATCCAGA ATGACCCACG CTATGCCATC 
CTCAATGAGC CTTTCAAGGG TGAAATGAAC CGTGGCAATT TCTTAGAGAT CAAGAATAAA 
TTTCTAGCTC GAAGGTTTAA GCTCTTAGAA CAAGCTCTGG TGATTGAGGA ACAGCTGCGC 
CGGGCTGCTT ACTTGAACAT GTCAGAAGAC CCTTCTCACC CTTCCATGGC CCTCAACACC 
CGCTTTGCTG AGGTGGAGTG TTTGGCGGAA AGTCATCAGC ACCTGTCCAA GGAGTCAATG 
GCAGGAAACA AGCCAGCCAA TGCAGTCCTG CACAAAGGTA TTCTGAAACA GCTGGAAGAA 
CTGCTGAGTG ACATGAAAGC TGATGTGACT CGACTCCCAG CTACCATTGC CCGAATTCCC 
CCAGTTGCTG TGAGGTTACA GATGTCAGAG CGTAACATTC TCAGCCGCCT GGCAAACCGG 
GCACCCGAAC CTACCCCACA GCAGGTAGCC CAGCAGCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHD4 ( XM_017018734.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016874223.1 Homo sapiens chromodomain helicase DNA binding protein 4 (CHD4), transcript variant X20, mRNA.

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CloneID	OHu83479
Clone ID Related Accession (Same CDS sequence)	XM_017018734.1
Accession Version	XM_017018734.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5679bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-25
Organism	Homo sapiens(human)
Product	chromodomain-helicase-DNA-binding protein 4 isoform X20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCGG GCCTGGGCTC CCCGTCCCCC TGCTCGGCGG GCAGTGAGGA GGAGGATATG 
GATGCACTTT TGAACAACAG CCTGCCCCCA CCCCACCCAG AAAATGAAGA GGACCCAGAA 
GAGGATTTGT CAGAAACAGA GACTCCAAAG CTCAAGAAGA AGAAAAAGCC TAAGAAACCT 
CGGGACCCTA AAATCCCTAA GAGCAAGCGC CAAAAAAAGG AGCTGGGGGA CAGCTCTGGG 
GAGGGGCCAG AGTTTGTGGA GGAGGAGGAA GAGGTGGCTC TGCGCTCAGA CAGTGAGGGC 
AGCGACTATA CTCCTGGCAA GAAGAAGAAG AAGAAGCTTG GACCTAAGAA AGAGAAGAAG 
AGCAAATCCA AGCGGAAGGA GGAGGAGGAG GAGGAGGATG ATGATGATGA TTCAAAGGAG 
CCTAAATCAT CTGCTCAGCT CCTGGAAGAC TGGGGCATGG AAGACATTGA CCACGTGTTC 
TCAGAGGAGG ATTATCGAAC CCTCACCAAC TACAAGGCCT TCAGCCAGTT TGTCAGACCC 
CTCATTGCTG CCAAAAATCC CAAGATTGCT GTCTCCAAGA TGATGATGGT TTTGGGTGCA 
AAATGGCGGG AGTTCAGTAC CAATAACCCC TTCAAAGGCA GTTCTGGGGC ATCAGTGGCA 
GCTGCGGCAG CAGCAGCGGT AGCTGTGGTG GAGAGCATGG TGACAGCCAC TGAGGTTGCA 
CCACCACCTC CCCCTGTGGA GGTGCCTATC CGCAAGGCCA AGACCAAGGA GGGCAAAGGT 
CCCAATGCTC GGAGGAAGCC CAAGGGCAGC CCTCGTGTAC CTGATGCCAA GAAGCCTAAA 
CCCAAGAAAG TAGCTCCCCT GAAAATCAAG CTGGGAGGTT TTGGTTCCAA GCGTAAGAGA 
TCCTCGAGTG AGGATGATGA CTTAGATGTG GAATCTGACT TCGATGATGC CAGTATCAAT 
AGCTATTCTG TTTCTGATGG TTCCACCAGC CGTAGTAGCC GCAGCCGCAA GAAACTCCGA 
ACCACTAAAA AGAAAAAGAA AGACCACCAG GACTATTGCG AGGTGTGCCA GCAAGGCGGT 
GAGATCATCC TGTGTGATAC CTGTCCCCGT GCTTACCACA TGGTCTGCCT GGATCCCGAC 
ATGGAGAAGG CTCCCGAGGG CAAGTGGAGC TGCCCACACT GCGAGAAGGA AGGCATCCAG 
TGGGAAGCTA AAGAGGACAA TTCGGAGGGT GAGGAGATCC TGGAAGAGGT TGGGGGAGAC 
CTCGAAGAGG AGGATGACCA CCATATGGAA TTCTGTCGGG TCTGCAAGGA TGGTGGGGAA 
CTGCTCTGCT GTGATACCTG TCCTTCTTCC TACCACATCC ACTGCCTGAA TCCCCCACTT 
CCAGAGATCC CCAACGGTGA ATGGCTCTGT CCCCGTTGTA CGTGTCCAGC TCTGAAGGGC 
AAAGTGCAGA AGATCCTAAT CTGGAAGTGG GGTCAGCCAC CATCTCCCAC ACCAGTGCCT 
CGGCCTCCAG ATGCTGATCC CAACACGCCC TCCCCAAAGC CCTTGGAGGG GCGGCCAGAG 
CGGCAGTTCT TTGTGAAATG GCAAGGCATG TCTTACTGGC ACTGCTCCTG GGTTTCTGAA 
CTGCAGCTGG AGCTGCACTG TCAGGTGATG TTCCGAAACT ATCAGCGGAA GAATGATATG 
GATGAGCCAC CTTCTGGGGA CTTTGGTGGT GATGAAGAGA AAAGCCGAAA GCGAAAGAAC 
AAGGACCCTA AATTTGCAGA GATGGAGGAA CGCTTCTATC GCTATGGGAT AAAACCCGAG 
TGGATGATGA TCCACCGAAT CCTCAACCAC AGTGTGGACA AGAAGGGCCA CGTCCACTAC 
TTGATCAAGT GGCGGGACTT ACCTTACGAT CAGGCTTCTT GGGAGAGTGA GGATGTGGAG 
ATCCAGGATT ACGACCTGTT CAAGCAGAGC TATTGGAATC ACAGGGAGTT AATGAGGGGT 
GAGGAAGGCC GACCAGGCAA GAAGCTCAAG AAGGTGAAGC TTCGGAAGTT GGAGAGGCCT 
CCAGAAACGC CAACAGTTGA TCCAACAGTG AAGTATGAGC GACAGCCAGA GTACCTGGAT 
GCTACAGGTG GAACCCTGCA CCCCTATCAA ATGGAGGGCC TGAATTGGTT GCGCTTCTCC 
TGGGCTCAGG GCACTGACAC CATCTTGGCT GATGAGATGG GCCTTGGGAA AACTGTACAG 
ACAGCAGTCT TCCTGTATTC CCTTTACAAG GAGGGTCATT CCAAAGGCCC CTTCCTAGTG 
AGCGCCCCTC TTTCTACCAT CATCAACTGG GAGCGGGAGT TTGAAATGTG GGCTCCAGAC 
ATGTATGTCG TAACCTATGT GGGTGACAAG GACAGCCGTG CCATCATCCG AGAGAATGAG 
TTCTCCTTTG AAGACAATGC CATTCGTGGT GGCAAGAAGG CCTCCCGCAT GAAGAAAGAG 
GCATCTGTGA AATTCCATGT GCTGCTGACA TCCTATGAAT TGATCACCAT TGACATGGCT 
ATTTTGGGCT CTATTGATTG GGCCTGCCTC ATCGTGGATG AAGCCCATCG GCTGAAGAAC 
AATCAGTCTA AGTTCTTCCG GGTATTGAAT GGTTACTCAC TCCAGCACAA GCTGTTGCTG 
ACTGGGACAC CATTACAAAA CAATCTGGAA GAGTTGTTTC ATCTGCTCAA CTTTCTCACC 
CCCGAGAGGT TCCACAATTT GGAAGGTTTT TTGGAGGAGT TTGCTGACAT TGCCAAGGAG 
GACCAGATAA AAAAACTGCA TGACATGCTG GGGCCGCACA TGTTGCGGCG GCTCAAAGCC 
GATGTGTTCA AGAACATGCC CTCCAAGACA GAACTAATTG TGCGTGTGGA GCTGAGCCCT 
ATGCAGAAGA AATACTACAA GTACATCCTC ACTCGAAATT TTGAAGCACT CAATGCCCGA 
GGTGGTGGCA ACCAGGTGTC TCTGCTGAAT GTGGTGATGG ATCTTAAGAA GTGCTGCAAC 
CATCCATACC TCTTCCCTGT GGCTGCAATG GAAGCTCCTA AGATGCCTAA TGGCATGTAT 
GATGGCAGTG CCCTAATCAG AGCATCTGGG AAATTATTGC TGCTGCAGAA AATGCTCAAG 
AACCTTAAGG AGGGTGGGCA TCGTGTACTC ATCTTTTCCC AGATGACCAA GATGCTAGAC 
CTGCTAGAGG ATTTCTTGGA ACATGAAGGT TATAAATACG AACGCATCGA TGGTGGAATC 
ACTGGGAACA TGCGGCAAGA GGCCATTGAC CGCTTCAATG CACCGGGTGC TCAGCAGTTC 
TGCTTCTTGC TTTCCACTCG AGCTGGGGGC CTTGGAATCA ATCTGGCCAC TGCTGACACA 
GTTATTATCT ATGACTCTGA CTGGAACCCC CATAATGACA TTCAGGCCTT TAGCAGAGCT 
CACCGGATTG GGCAAAATAA AAAGGTAATG ATCTACCGGT TTGTGACCCG TGCGTCAGTG 
GAGGAGCGCA TCACGCAGGT GGCAAAGAAG AAAATGATGC TGACGCATCT AGTGGTGCGG 
CCTGGGCTGG GCTCCAAGAC TGGATCTATG TCCAAACAGG AGCTTGATGA TATCCTCAAA 
TTTGGCACTG AGGAACTATT CAAGGATGAA GCCACTGATG GAGGAGGAGA CAACAAAGAG 
GGAGAAGATA GCAGTGTTAT CCACTACGAT GATAAGGCCA TTGAACGGCT GCTAGACCGT 
AACCAGGATG AGACTGAAGA CACAGAATTG CAGGGCATGA ATGAATATTT GAGCTCATTC 
AAAGTGGCCC AGTATGTGGT ACGGGAAGAA GAAATGGGGG AGGAAGAGGA GGTAGAACGG 
GAAATCATTA AACAGGAAGA AAGTGTGGAT CCTGACTACT GGGAGAAATT GCTGCGGCAC 
CATTATGAGC AGCAGCAAGA AGATCTAGCC CGAAATCTGG GCAAAGGAAA AAGAATCCGT 
AAACAGGTCA ACTACAATGA TGGCTCCCAG GAGGACCGAG ATTGGCAGGA CGACCAGTCC 
GACAACCAGT CCGATTACTC AGTGGCTTCA GAGGAAGGTG ATGAAGACTT TGATGAACGT 
TCAGAAGCTC CCCGTAGGCC CAGTCGTAAG GGCCTGCGGA ATGATAAAGA TAAGCCATTG 
CCTCCTCTGT TGGCCCGTGT TGGTGGGAAT ATTGAAGTAC TTGGTTTTAA TGCTCGTCAG 
CGAAAAGCCT TTCTTAATGC AATTATGCGA TATGGTATGC CACCTCAGGA TGCTTTTACT 
ACCCAGTGGC TTGTAAGAGA CCTGCGAGGC AAATCAGAGA AAGAGTTCAA GGCATATGTC 
TCTCTTTTCA TGCGGCATTT ATGTGAGCCG GGGGCAGATG GGGCTGAGAC CTTTGCTGAT 
GGTGTCCCCC GAGAAGGCCT GTCTCGCCAG CATGTCCTTA CTAGAATTGG TGTTATGTCT 
TTGATTCGCA AGAAGGTTCA GGAGTTTGAA CATGTTAATG GGCGCTGGAG CATGCCTGAA 
CTGGCTGAGG TGGAGGAAAA CAAGAAGATG TCCCAGCCAG GGTCACCCTC CCCAAAAACT 
CCTACACCCT CCACTCCAGG GGACACGCAG CCCAACACTC CTGCACCTGT CCCACCTGCT 
GAAGATGGGA TAAAAATAGA GGAAAATAGC CTCAAAGAAG AAGAGAGCAT AGAAGGAGAA 
AAGGAGGTTA AATCTACAGC CCCTGAGACT GCCATTGAGT GTACACAGGC CCCTGCCCCT 
GCCTCAGAGG ATGAAAAGGT CGTTGTTGAA CCCCCTGAGG GAGAGGAGAA AGTGGAAAAG 
GCAGAGGTGA AGGAGAGAAC AGAGGAACCT ATGGAGACAG AGCCCAAAGG TGCTGCTGAT 
GTAGAGAAGG TGGAGGAAAA GTCAGCAATA GATCTGACCC CTATTGTGGT AGAAGACAAA 
GAAGAGAAGA AAGAAGAAGA AGAGAAAAAA GAGGTGATGC TTCAGAATGG AGAGACCCCC 
AAGGACCTGA ATGATGAGAA ACAGAAGAAA AATATTAAAC AACGTTTCAT GTTTAACATT 
GCAGATGGTG GTTTTACTGA GTTGCACTCC CTTTGGCAGA ATGAAGAGCG GGCAGCCACA 
GTTACCAAGA AGACTTATGA GATCTGGCAT CGACGGCATG ACTACTGGCT GCTAGCCGGC 
ATTATAAACC ATGGCTATGC CCGGTGGCAA GACATCCAGA ATGACCCACG CTATGCCATC 
CTCAATGAGC CTTTCAAGGG TGAAATGAAC CGTGGCAATT TCTTAGAGAT CAAGAATAAA 
TTTCTAGCTC GAAGGTTTAA GCTCTTAGAA CAAGCTCTGG TGATTGAGGA ACAGCTGCGC 
CGGGCTGCTT ACTTGAACAT GTCAGAAGAC CCTTCTCACC CTTCCATGGC CCTCAACACC 
CGCTTTGCTG AGGTGGAGTG TTTGGCGGAA AGTCATCAGC ACCTGTCCAA GGAGTCAATG 
GCAGGAAACA AGCCAGCCAA TGCAGTCCTG CACAAAGTTC TGAAACAGCT GGAAGAACTG 
CTGAGTGACA TGAAAGCTGA TGTGACTCGA CTCCCAGCTA CCATTGCCCG AATTCCCCCA 
GTTGCTGTGA GGTTACAGAT GTCAGAGCGT AACATTCTCA GCCGCCTGGC AAACCGGGCA 
CCCGAACCTA CCCCACAGCA GGTAGCCCAG CAGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016874223.1
CDS	165..5843
Translation	MASGLGSPSPCSAGSEEEDMDALLNNSLPPPHPENEEDPEEDLSETETPKLKKKKKPKKPRDPKIPKSKRQKKELGDSSGEGPEFVEEEEEVALRSDSEGSDYTPGKKKKKKLGPKKEKKSKSKRKEEEEEEDDDDDSKEPKSSAQLLEDWGMEDIDHVFSEEDYRTLTNYKAFSQFVRPLIAAKNPKIAVSKMMMVLGAKWREFSTNNPFKGSSGASVAAAAAAAVAVVESMVTATEVAPPPPPVEVPIRKAKTKEGKGPNARRKPKGSPRVPDAKKPKPKKVAPLKIKLGGFGSKRKRSSSEDDDLDVESDFDDASINSYSVSDGSTSRSSRSRKKLRTTKKKKKDHQDYCEVCQQGGEIILCDTCPRAYHMVCLDPDMEKAPEGKWSCPHCEKEGIQWEAKEDNSEGEEILEEVGGDLEEEDDHHMEFCRVCKDGGELLCCDTCPSSYHIHCLNPPLPEIPNGEWLCPRCTCPALKGKVQKILIWKWGQPPSPTPVPRPPDADPNTPSPKPLEGRPERQFFVKWQGMSYWHCSWVSELQLELHCQVMFRNYQRKNDMDEPPSGDFGGDEEKSRKRKNKDPKFAEMEERFYRYGIKPEWMMIHRILNHSVDKKGHVHYLIKWRDLPYDQASWESEDVEIQDYDLFKQSYWNHRELMRGEEGRPGKKLKKVKLRKLERPPETPTVDPTVKYERQPEYLDATGGTLHPYQMEGLNWLRFSWAQGTDTILADEMGLGKTVQTAVFLYSLYKEGHSKGPFLVSAPLSTIINWEREFEMWAPDMYVVTYVGDKDSRAIIRENEFSFEDNAIRGGKKASRMKKEASVKFHVLLTSYELITIDMAILGSIDWACLIVDEAHRLKNNQSKFFRVLNGYSLQHKLLLTGTPLQNNLEELFHLLNFLTPERFHNLEGFLEEFADIAKEDQIKKLHDMLGPHMLRRLKADVFKNMPSKTELIVRVELSPMQKKYYKYILTRNFEALNARGGGNQVSLLNVVMDLKKCCNHPYLFPVAAMEAPKMPNGMYDGSALIRASGKLLLLQKMLKNLKEGGHRVLIFSQMTKMLDLLEDFLEHEGYKYERIDGGITGNMRQEAIDRFNAPGAQQFCFLLSTRAGGLGINLATADTVIIYDSDWNPHNDIQAFSRAHRIGQNKKVMIYRFVTRASVEERITQVAKKKMMLTHLVVRPGLGSKTGSMSKQELDDILKFGTEELFKDEATDGGGDNKEGEDSSVIHYDDKAIERLLDRNQDETEDTELQGMNEYLSSFKVAQYVVREEEMGEEEEVEREIIKQEESVDPDYWEKLLRHHYEQQQEDLARNLGKGKRIRKQVNYNDGSQEDRDWQDDQSDNQSDYSVASEEGDEDFDERSEAPRRPSRKGLRNDKDKPLPPLLARVGGNIEVLGFNARQRKAFLNAIMRYGMPPQDAFTTQWLVRDLRGKSEKEFKAYVSLFMRHLCEPGADGAETFADGVPREGLSRQHVLTRIGVMSLIRKKVQEFEHVNGRWSMPELAEVEENKKMSQPGSPSPKTPTPSTPGDTQPNTPAPVPPAEDGIKIEENSLKEEESIEGEKEVKSTAPETAIECTQAPAPASEDEKVVVEPPEGEEKVEKAEVKERTEEPMETEPKGAADVEKVEEKSAIDLTPIVVEDKEEKKEEEEKKEVMLQNGETPKDLNDEKQKKNIKQRFMFNIADGGFTELHSLWQNEERAATVTKKTYEIWHRRHDYWLLAGIINHGYARWQDIQNDPRYAILNEPFKGEMNRGNFLEIKNKFLARRFKLLEQALVIEEQLRRAAYLNMSEDPSHPSMALNTRFAEVECLAESHQHLSKESMAGNKPANAVLHKVLKQLEELLSDMKADVTRLPATIARIPPVAVRLQMSERNILSRLANRAPEPTPQQVAQQQ

Target ORF information:

RefSeq Version	XM_017018734.1
Organism	Homo sapiens(human)
Definition	Homo sapiens chromodomain helicase DNA binding protein 4 (CHD4), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017018734.1

ORF Insert Sequence:

ATGGCGTCGG GCCTGGGCTC CCCGTCCCCC TGCTCGGCGG GCAGTGAGGA GGAGGATATG 
GATGCACTTT TGAACAACAG CCTGCCCCCA CCCCACCCAG AAAATGAAGA GGACCCAGAA 
GAGGATTTGT CAGAAACAGA GACTCCAAAG CTCAAGAAGA AGAAAAAGCC TAAGAAACCT 
CGGGACCCTA AAATCCCTAA GAGCAAGCGC CAAAAAAAGG AGCTGGGGGA CAGCTCTGGG 
GAGGGGCCAG AGTTTGTGGA GGAGGAGGAA GAGGTGGCTC TGCGCTCAGA CAGTGAGGGC 
AGCGACTATA CTCCTGGCAA GAAGAAGAAG AAGAAGCTTG GACCTAAGAA AGAGAAGAAG 
AGCAAATCCA AGCGGAAGGA GGAGGAGGAG GAGGAGGATG ATGATGATGA TTCAAAGGAG 
CCTAAATCAT CTGCTCAGCT CCTGGAAGAC TGGGGCATGG AAGACATTGA CCACGTGTTC 
TCAGAGGAGG ATTATCGAAC CCTCACCAAC TACAAGGCCT TCAGCCAGTT TGTCAGACCC 
CTCATTGCTG CCAAAAATCC CAAGATTGCT GTCTCCAAGA TGATGATGGT TTTGGGTGCA 
AAATGGCGGG AGTTCAGTAC CAATAACCCC TTCAAAGGCA GTTCTGGGGC ATCAGTGGCA 
GCTGCGGCAG CAGCAGCGGT AGCTGTGGTG GAGAGCATGG TGACAGCCAC TGAGGTTGCA 
CCACCACCTC CCCCTGTGGA GGTGCCTATC CGCAAGGCCA AGACCAAGGA GGGCAAAGGT 
CCCAATGCTC GGAGGAAGCC CAAGGGCAGC CCTCGTGTAC CTGATGCCAA GAAGCCTAAA 
CCCAAGAAAG TAGCTCCCCT GAAAATCAAG CTGGGAGGTT TTGGTTCCAA GCGTAAGAGA 
TCCTCGAGTG AGGATGATGA CTTAGATGTG GAATCTGACT TCGATGATGC CAGTATCAAT 
AGCTATTCTG TTTCTGATGG TTCCACCAGC CGTAGTAGCC GCAGCCGCAA GAAACTCCGA 
ACCACTAAAA AGAAAAAGAA AGACCACCAG GACTATTGCG AGGTGTGCCA GCAAGGCGGT 
GAGATCATCC TGTGTGATAC CTGTCCCCGT GCTTACCACA TGGTCTGCCT GGATCCCGAC 
ATGGAGAAGG CTCCCGAGGG CAAGTGGAGC TGCCCACACT GCGAGAAGGA AGGCATCCAG 
TGGGAAGCTA AAGAGGACAA TTCGGAGGGT GAGGAGATCC TGGAAGAGGT TGGGGGAGAC 
CTCGAAGAGG AGGATGACCA CCATATGGAA TTCTGTCGGG TCTGCAAGGA TGGTGGGGAA 
CTGCTCTGCT GTGATACCTG TCCTTCTTCC TACCACATCC ACTGCCTGAA TCCCCCACTT 
CCAGAGATCC CCAACGGTGA ATGGCTCTGT CCCCGTTGTA CGTGTCCAGC TCTGAAGGGC 
AAAGTGCAGA AGATCCTAAT CTGGAAGTGG GGTCAGCCAC CATCTCCCAC ACCAGTGCCT 
CGGCCTCCAG ATGCTGATCC CAACACGCCC TCCCCAAAGC CCTTGGAGGG GCGGCCAGAG 
CGGCAGTTCT TTGTGAAATG GCAAGGCATG TCTTACTGGC ACTGCTCCTG GGTTTCTGAA 
CTGCAGCTGG AGCTGCACTG TCAGGTGATG TTCCGAAACT ATCAGCGGAA GAATGATATG 
GATGAGCCAC CTTCTGGGGA CTTTGGTGGT GATGAAGAGA AAAGCCGAAA GCGAAAGAAC 
AAGGACCCTA AATTTGCAGA GATGGAGGAA CGCTTCTATC GCTATGGGAT AAAACCCGAG 
TGGATGATGA TCCACCGAAT CCTCAACCAC AGTGTGGACA AGAAGGGCCA CGTCCACTAC 
TTGATCAAGT GGCGGGACTT ACCTTACGAT CAGGCTTCTT GGGAGAGTGA GGATGTGGAG 
ATCCAGGATT ACGACCTGTT CAAGCAGAGC TATTGGAATC ACAGGGAGTT AATGAGGGGT 
GAGGAAGGCC GACCAGGCAA GAAGCTCAAG AAGGTGAAGC TTCGGAAGTT GGAGAGGCCT 
CCAGAAACGC CAACAGTTGA TCCAACAGTG AAGTATGAGC GACAGCCAGA GTACCTGGAT 
GCTACAGGTG GAACCCTGCA CCCCTATCAA ATGGAGGGCC TGAATTGGTT GCGCTTCTCC 
TGGGCTCAGG GCACTGACAC CATCTTGGCT GATGAGATGG GCCTTGGGAA AACTGTACAG 
ACAGCAGTCT TCCTGTATTC CCTTTACAAG GAGGGTCATT CCAAAGGCCC CTTCCTAGTG 
AGCGCCCCTC TTTCTACCAT CATCAACTGG GAGCGGGAGT TTGAAATGTG GGCTCCAGAC 
ATGTATGTCG TAACCTATGT GGGTGACAAG GACAGCCGTG CCATCATCCG AGAGAATGAG 
TTCTCCTTTG AAGACAATGC CATTCGTGGT GGCAAGAAGG CCTCCCGCAT GAAGAAAGAG 
GCATCTGTGA AATTCCATGT GCTGCTGACA TCCTATGAAT TGATCACCAT TGACATGGCT 
ATTTTGGGCT CTATTGATTG GGCCTGCCTC ATCGTGGATG AAGCCCATCG GCTGAAGAAC 
AATCAGTCTA AGTTCTTCCG GGTATTGAAT GGTTACTCAC TCCAGCACAA GCTGTTGCTG 
ACTGGGACAC CATTACAAAA CAATCTGGAA GAGTTGTTTC ATCTGCTCAA CTTTCTCACC 
CCCGAGAGGT TCCACAATTT GGAAGGTTTT TTGGAGGAGT TTGCTGACAT TGCCAAGGAG 
GACCAGATAA AAAAACTGCA TGACATGCTG GGGCCGCACA TGTTGCGGCG GCTCAAAGCC 
GATGTGTTCA AGAACATGCC CTCCAAGACA GAACTAATTG TGCGTGTGGA GCTGAGCCCT 
ATGCAGAAGA AATACTACAA GTACATCCTC ACTCGAAATT TTGAAGCACT CAATGCCCGA 
GGTGGTGGCA ACCAGGTGTC TCTGCTGAAT GTGGTGATGG ATCTTAAGAA GTGCTGCAAC 
CATCCATACC TCTTCCCTGT GGCTGCAATG GAAGCTCCTA AGATGCCTAA TGGCATGTAT 
GATGGCAGTG CCCTAATCAG AGCATCTGGG AAATTATTGC TGCTGCAGAA AATGCTCAAG 
AACCTTAAGG AGGGTGGGCA TCGTGTACTC ATCTTTTCCC AGATGACCAA GATGCTAGAC 
CTGCTAGAGG ATTTCTTGGA ACATGAAGGT TATAAATACG AACGCATCGA TGGTGGAATC 
ACTGGGAACA TGCGGCAAGA GGCCATTGAC CGCTTCAATG CACCGGGTGC TCAGCAGTTC 
TGCTTCTTGC TTTCCACTCG AGCTGGGGGC CTTGGAATCA ATCTGGCCAC TGCTGACACA 
GTTATTATCT ATGACTCTGA CTGGAACCCC CATAATGACA TTCAGGCCTT TAGCAGAGCT 
CACCGGATTG GGCAAAATAA AAAGGTAATG ATCTACCGGT TTGTGACCCG TGCGTCAGTG 
GAGGAGCGCA TCACGCAGGT GGCAAAGAAG AAAATGATGC TGACGCATCT AGTGGTGCGG 
CCTGGGCTGG GCTCCAAGAC TGGATCTATG TCCAAACAGG AGCTTGATGA TATCCTCAAA 
TTTGGCACTG AGGAACTATT CAAGGATGAA GCCACTGATG GAGGAGGAGA CAACAAAGAG 
GGAGAAGATA GCAGTGTTAT CCACTACGAT GATAAGGCCA TTGAACGGCT GCTAGACCGT 
AACCAGGATG AGACTGAAGA CACAGAATTG CAGGGCATGA ATGAATATTT GAGCTCATTC 
AAAGTGGCCC AGTATGTGGT ACGGGAAGAA GAAATGGGGG AGGAAGAGGA GGTAGAACGG 
GAAATCATTA AACAGGAAGA AAGTGTGGAT CCTGACTACT GGGAGAAATT GCTGCGGCAC 
CATTATGAGC AGCAGCAAGA AGATCTAGCC CGAAATCTGG GCAAAGGAAA AAGAATCCGT 
AAACAGGTCA ACTACAATGA TGGCTCCCAG GAGGACCGAG ATTGGCAGGA CGACCAGTCC 
GACAACCAGT CCGATTACTC AGTGGCTTCA GAGGAAGGTG ATGAAGACTT TGATGAACGT 
TCAGAAGCTC CCCGTAGGCC CAGTCGTAAG GGCCTGCGGA ATGATAAAGA TAAGCCATTG 
CCTCCTCTGT TGGCCCGTGT TGGTGGGAAT ATTGAAGTAC TTGGTTTTAA TGCTCGTCAG 
CGAAAAGCCT TTCTTAATGC AATTATGCGA TATGGTATGC CACCTCAGGA TGCTTTTACT 
ACCCAGTGGC TTGTAAGAGA CCTGCGAGGC AAATCAGAGA AAGAGTTCAA GGCATATGTC 
TCTCTTTTCA TGCGGCATTT ATGTGAGCCG GGGGCAGATG GGGCTGAGAC CTTTGCTGAT 
GGTGTCCCCC GAGAAGGCCT GTCTCGCCAG CATGTCCTTA CTAGAATTGG TGTTATGTCT 
TTGATTCGCA AGAAGGTTCA GGAGTTTGAA CATGTTAATG GGCGCTGGAG CATGCCTGAA 
CTGGCTGAGG TGGAGGAAAA CAAGAAGATG TCCCAGCCAG GGTCACCCTC CCCAAAAACT 
CCTACACCCT CCACTCCAGG GGACACGCAG CCCAACACTC CTGCACCTGT CCCACCTGCT 
GAAGATGGGA TAAAAATAGA GGAAAATAGC CTCAAAGAAG AAGAGAGCAT AGAAGGAGAA 
AAGGAGGTTA AATCTACAGC CCCTGAGACT GCCATTGAGT GTACACAGGC CCCTGCCCCT 
GCCTCAGAGG ATGAAAAGGT CGTTGTTGAA CCCCCTGAGG GAGAGGAGAA AGTGGAAAAG 
GCAGAGGTGA AGGAGAGAAC AGAGGAACCT ATGGAGACAG AGCCCAAAGG TGCTGCTGAT 
GTAGAGAAGG TGGAGGAAAA GTCAGCAATA GATCTGACCC CTATTGTGGT AGAAGACAAA 
GAAGAGAAGA AAGAAGAAGA AGAGAAAAAA GAGGTGATGC TTCAGAATGG AGAGACCCCC 
AAGGACCTGA ATGATGAGAA ACAGAAGAAA AATATTAAAC AACGTTTCAT GTTTAACATT 
GCAGATGGTG GTTTTACTGA GTTGCACTCC CTTTGGCAGA ATGAAGAGCG GGCAGCCACA 
GTTACCAAGA AGACTTATGA GATCTGGCAT CGACGGCATG ACTACTGGCT GCTAGCCGGC 
ATTATAAACC ATGGCTATGC CCGGTGGCAA GACATCCAGA ATGACCCACG CTATGCCATC 
CTCAATGAGC CTTTCAAGGG TGAAATGAAC CGTGGCAATT TCTTAGAGAT CAAGAATAAA 
TTTCTAGCTC GAAGGTTTAA GCTCTTAGAA CAAGCTCTGG TGATTGAGGA ACAGCTGCGC 
CGGGCTGCTT ACTTGAACAT GTCAGAAGAC CCTTCTCACC CTTCCATGGC CCTCAACACC 
CGCTTTGCTG AGGTGGAGTG TTTGGCGGAA AGTCATCAGC ACCTGTCCAA GGAGTCAATG 
GCAGGAAACA AGCCAGCCAA TGCAGTCCTG CACAAAGTTC TGAAACAGCT GGAAGAACTG 
CTGAGTGACA TGAAAGCTGA TGTGACTCGA CTCCCAGCTA CCATTGCCCG AATTCCCCCA 
GTTGCTGTGA GGTTACAGAT GTCAGAGCGT AACATTCTCA GCCGCCTGGC AAACCGGGCA 
CCCGAACCTA CCCCACAGCA GGTAGCCCAG CAGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

CHD4-mediated loss of E-cadherin determines metastatic ability in triple-negative breast cancer cells.
Experimental cell research(2018 Jan)
Luo CW,Wu CC,Chang SJ,Chang TM,Chen TY,Chai CY,Chang CL,Hou MF,Pan MR

Transcription factor 19 interacts with histone 3 lysine 4 trimethylation and controls gluconeogenesis via the nucleosome-remodeling-deacetylase complex.
The Journal of biological chemistry292(50)20362-20378(2017 Dec)
Sen S,Sanyal S,Srivastava DK,Dasgupta D,Roy S,Das C

EZH2-, CHD4-, and IDH-linked epigenetic perturbation and its association with survival in glioma patients.
Journal of molecular cell biology(2017 Dec)
Zhang L,Liu Y,Wang M,Wu Z,Li N,Zhang J,Yang C

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CHD4 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

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Target ORF information:

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ORF Insert Sequence:

Target ORF information:

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