TOPBP1 cDNA ORF clone, Homo sapiens(human)

The following TOPBP1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TOPBP1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu16438 NM_007027.4
Latest version!
Homo sapiens DNA topoisomerase II binding protein 1 (TOPBP1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$579.50-$811.30
$1159.00
OHu16438 XM_017005636.2
Latest version!
Homo sapiens DNA topoisomerase II binding protein 1 (TOPBP1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$579.50-$811.30
$1159.00
OHu93765 XM_017005637.2
Latest version!
Homo sapiens DNA topoisomerase II binding protein 1 (TOPBP1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 15-17 $559.30
$799.00
OHu64678 XM_011512357.3
Latest version!
Homo sapiens DNA topoisomerase II binding protein 1 (TOPBP1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
OHu37681 NM_001363889.2
Latest version!
Homo sapiens DNA topoisomerase II binding protein 1 (TOPBP1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
OHu37681 XM_005247076.3
Latest version!
Homo sapiens topoisomerase (DNA) II binding protein 1 (TOPBP1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
View Old Accession Versions >>
OHu93765 XM_017005637.1 Homo sapiens topoisomerase (DNA) II binding protein 1 (TOPBP1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 15-17 $559.30
$799.00
OHu16438 XM_017005636.1 Homo sapiens topoisomerase (DNA) II binding protein 1 (TOPBP1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$579.50-$811.30
$1159.00
OHu64678 XM_011512357.2 Homo sapiens topoisomerase (DNA) II binding protein 1 (TOPBP1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
OHu37681 NM_001363889.1 Homo sapiens DNA topoisomerase II binding protein 1 (TOPBP1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
OHu16438 NM_007027.3 Homo sapiens DNA topoisomerase II binding protein 1 (TOPBP1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$579.50-$811.30
$1159.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu16438
    Clone ID Related Accession (Same CDS sequence) XM_017005636.1 , XM_017005636.2 , NM_007027.3 , NM_007027.4
    Accession Version NM_007027.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4569bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-17
    Organism Homo sapiens(human)
    Product DNA topoisomerase 2-binding protein 1 isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC032633.1, BC126209.1 and BC151237.1. On Jul 25, 2008 this sequence version replaced NM_007027.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC151237.1, SRR1660803.23079.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGTCCAGAA ATGACAAAGA ACCGTTTTTT GTGAAGTTTT TAAAGTCTTC AGACAATTCC 
    AAATGTTTTT TTAAAGCTCT CGAGTCCATA AAAGAATTCC AATCAGAAGA ATATCTTCAG
    ATTATTACAG AAGAAGAGGC ATTGAAGATA AAGGAGAATG ATAGATCACT TTATATCTGT
    GACCCTTTTA GTGGCGTTGT CTTTGATCAC CTCAAAAAGC TTGGCTGCAG AATTGTTGGT
    CCTCAAGTAG TCATATTTTG TATGCACCAC CAGCGATGTG TCCCAAGAGC CGAACATCCA
    GTTTATAATA TGGTTATGTC TGATGTAACC ATATCTTGTA CAAGTCTGGA AAAAGAAAAA
    AGGGAAGAAG TTCATAAATA TGTACAAATG ATGGGCGGAC GAGTATACAG AGACCTTAAT
    GTATCAGTAA CTCACCTTAT TGCAGGAGAA GTTGGTAGCA AAAAATATTT AGTTGCTGCA
    AACCTGAAGA AACCTATTTT GCTTCCCTCT TGGATAAAAA CACTTTGGGA GAAGTCACAA
    GAGAAAAAAA TAACTAGATA TACTGATATA AACATGGAAG ATTTCAAGTG TCCTATTTTT
    CTTGGTTGCA TAATCTGTGT GACTGGCTTA TGTGGCTTAG ACAGGAAAGA AGTTCAGCAA
    CTCACAGTTA AGCATGGAGG TCAATACATG GGACAATTGA AAATGAATGA ATGTACACAC
    CTCATTGTGC AAGAACCAAA AGGTCAGAAG TATGAGTGTG CCAAGAGATG GAATGTACAC
    TGTGTGACCA CACAGTGGTT TTTTGACAGT ATTGAGAAAG GTTTTTGTCA GGATGAATCC
    ATATACAAGA CAGAACCTAG ACCAGAAGCA AAGACTATGC CCAATTCTTC AACTCCTACC
    AGCCAGATCA ACACAATTGA TAGTCGTACT CTTTCAGATG TCAGCAATAT TTCCAACATA
    AATGCAAGTT GCGTAAGTGA ATCAATATGT AATTCACTTA ACAGCAAACT GGAGCCTACA
    CTTGAAAATC TAGAAAATCT GGATGTCAGT GCATTTCAAG CACCTGAAGA TTTATTAGAT
    GGTTGTCGGA TATATCTTTG CGGTTTTAGT GGCAGAAAGC TAGATAAACT GAGAAGACTT
    ATTAACAGTG GAGGTGGAGT TCGTTTTAAC CAGCTAAATG AAGATGTAAC TCATGTTATT
    GTGGGAGATT ATGATGATGA ATTGAAGCAG TTTTGGAATA AATCAGCCCA CAGGCCTCAT
    GTAGTGGGAG CAAAGTGGTT GCTAGAGTGT TTCAGTAAAG GTTATATGCT TTCTGAAGAA
    CCATATATCC ATGCTAATTA CCAGCCAGTG GAAATTCCAG TTTCACATAA GCCTGAAAGT
    AAAGCAGCTC TTTTAAAAAA GAAGAACAGC AGCTTCTCTA AGAAAGACTT TGCTCCTAGT
    GAAAAGCATG AGCAAGCTGA TGAAGATCTG CTCTCTCAAT ATGAAAATGG TAGCTCCACA
    GTAGTTGAGG CTAAGACGTC TGAAGCCAGG CCCTTTAATG ATTCTACTCA TGCTGAGCCC
    TTGAATGATT CTACTCACAT TTCTTTGCAA GAAGAAAACC AGTCTTCTGT CAGTCATTGT
    GTCCCTGATG TTTCTACAAT TACTGAAGAA GGCTTATTTA GCCAAAAGAG TTTCCTTGTT
    TTGGGTTTTA GTAATGAAAA TGAATCTAAC ATCGCAAACA TCATAAAAGA AAATGCTGGG
    AAAATCATGT CCCTTCTGAG CAGAACTGTT GCGGATTATG CTGTGGTTCC TCTGCTGGGG
    TGTGAAGTGG AAGCCACTGT GGGAGAAGTT GTTACAAATA CATGGCTGGT TACTTGCATA
    GACTATCAGA CTTTGTTTGA TCCAAAGTCG AATCCTCTCT TCACACCAGT TCCAGTAATG
    ACAGGAATGA CTCCTTTAGA GGATTGTGTT ATTTCATTTA GCCAGTGTGC TGGAGCAGAA
    AAAGAGTCTT TAACATTCCT AGCAAACCTC CTTGGAGCAA GTGTTCAAGA ATACTTTGTT
    CGCAAATCCA ATGCAAAGAA AGGCATGTTT GCCAGTACTC ATCTTATACT GAAAGAACGT
    GGTGGCTCTA AATATGAAGC TGCAAAGAAG TGGAATTTAC CTGCCGTTAC TATAGCTTGG
    CTGTTGGAGA CTGCTAGAAC GGGAAAGAGA GCAGACGAAA GCCATTTTCT GATTGAAAAT
    TCAACTAAAG AAGAACGAAG TTTGGAAACA GAAATAACAA ATGGAATCAA TCTAAATTCA
    GATACTGCAG AGCATCCTGG CACACGCCTG CAAACTCACA GAAAAACCGT CGTTACACCT
    TTAGATATGA ACCGCTTTCA GAGTAAAGCT TTCCGTGCTG TGGTCTCACA ACATGCCAGA
    CAGGTCGCAG CCTCCCCAGC AGTAGGACAA CCACTTCAGA AGGAGCCCTC GTTACACCTG
    GATACACCAT CAAAATTCCT GTCCAAGGAC AAACTCTTCA AGCCTTCCTT TGATGTGAAG
    GATGCACTTG CAGCCTTGGA AACTCCAGGA CGTCCCAGCC AACAGAAAAG GAAACCGAGT
    ACGCCACTCT CAGAAGTTAT TGTCAAAAAC TTGCAACTTG CTTTGGCAAA TAGCTCTCGA
    AATGCTGTCG CTCTTTCTGC CAGCCCTCAA CTGAAAGAGG CCCAGTCAGA GAAGGAAGAA
    GCCCCAAAGC CACTTCACAA AGTAGTGGTA TGTGTTAGTA AAAAACTCAG TAAGAAGCAG
    AGTGAACTAA ATGGGATCGC AGCCTCTCTA GGAGCAGATT ACAGGTGGAG TTTTGATGAA
    ACAGTGACTC ATTTCATCTA TCAAGGGCGG CCAAATGACA CTAATCGGGA GTATAAATCT
    GTAAAAGAAA GAGGAGTACA CATTGTTTCC GAGCACTGGC TTTTAGATTG TGCCCAAGAG
    TGTAAACATC TTCCTGAATC TCTTTATCCA CATACTTATA ATCCCAAAAT GAGCTTGGAT
    ATCAGCGCAG TGCAAGATGG CCGGCTCTGT AATAGTCGAC TACTCTCAGC TGTGTCTTCA
    ACAAAGGATG ATGAGCCAGA TCCTTTGATT TTAGAAGAAA ATGATGTAGA CAATATGGCC
    ACCAATAATA AAGAGTCAGC ACCATCAAAT GGAAGTGGAA AGAATGACTC TAAAGGAGTT
    CTGACACAGA CCTTAGAGAT GAGAGAGAAC TTTCAGAAGC AGTTACAGGA GATAATGTCT
    GCAACATCAA TAGTGAAACC CCAAGGGCAG AGGACTTCCC TTTCAAGAAG TGGTTGTAAC
    AGCGCATCTT CAACCCCTGA CAGCACTCGC TCTGCTCGCA GTGGACGAAG TAGAGTCCTA
    GAGGCACTGA GGCAGTCTCG TCAGACAGTA CCTGATGTCA ACACAGAGCC TTCCCAAAAT
    GAACAGATCA TTTGGGATGA CCCTACAGCA AGGGAGGAGA GAGCAAGGCT TGCCAGCAAT
    TTGCAGTGGC CTAGTTGTCC CACACAATAC TCTGAGCTTC AGGTTGACAT TCAAAACTTG
    GAGGATTCTC CTTTTCAAAA GCCTTTACAT GATTCAGAAA TTGCTAAACA GGCTGTCTGT
    GATCCTGGAA ACATACGTGT GACTGAAGCT CCCAAACACC CAATCTCTGA AGAACTGGAA
    ACTCCCATAA AAGACAGCCA CCTGATCCCT ACGCCTCAAG CCCCCAGTAT TGCCTTTCCA
    CTCGCCAACC CCCCTGTGGC TCCGCACCCT AGAGAAAAGA TTATAACGAT AGAGGAGACT
    CATGAAGAAT TAAAAAAACA GTACATATTT CAGTTATCAT CTCTGAATCC TCAAGAACGT
    ATTGACTATT GTCATCTGAT TGAGAAACTA GGTGGATTGG TGATAGAAAA GCAGTGCTTT
    GATCCCACCT GTACACACAT TGTTGTGGGA CATCCACTTC GAAACGAGAA GTATTTAGCC
    TCAGTGGCAG CTGGGAAGTG GGTGCTTCAT CGCTCCTACC TTGAAGCCTG CAGGACTGCT
    GGACACTTCG TGCAGGAAGA AGACTATGAA TGGGGAAGTA GTTCCATACT TGATGTTCTG
    ACTGGAATCA ATGTACAGCA ACGAAGACTA GCACTTGCAG CAATGAGATG GAGAAAAAAA
    ATCCAGCAAA GACAAGAATC TGGCATTGTT GAGGGAGCAT TTAGTGGGTG GAAGGTTATT
    TTACATGTGG ATCAGTCTCG AGAAGCAGGC TTCAAACGCC TTCTTCAGTC AGGAGGAGCA
    AAGGTGCTAC CTGGTCATTC TGTACCTTTA TTTAAAGAGG CCACACATCT TTTTTCTGAC
    TTGAATAAAC TGAAACCAGA TGACTCAGGA GTTAATATAG CAGAAGCTGC TGCCCAGAAC
    GTGTACTGCT TGAGAACAGA ATACATTGCT GATTATCTCA TGCAGGAATC ACCTCCTCAT
    GTAGAAAATT ACTGTCTACC AGAAGCTATT TCATTTATTC AGAATAATAA GGAACTTGGG
    ACTGGATTAT CACAAAAGAG GAAAGCTCCT ACAGAAAAAA ATAAAATCAA ACGACCTAGA
    GTACACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_008958.2
    CDS133..4701
    Translation

    Target ORF information:

    RefSeq Version NM_007027.3
    Organism Homo sapiens(human)
    Definition Homo sapiens DNA topoisomerase II binding protein 1 (TOPBP1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_007027.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGTCCAGAA ATGACAAAGA ACCGTTTTTT GTGAAGTTTT TAAAGTCTTC AGACAATTCC 
    AAATGTTTTT TTAAAGCTCT CGAGTCCATA AAAGAATTCC AATCAGAAGA ATATCTTCAG
    ATTATTACAG AAGAAGAGGC ATTGAAGATA AAGGAGAATG ATAGATCACT TTATATCTGT
    GACCCTTTTA GTGGCGTTGT CTTTGATCAC CTCAAAAAGC TTGGCTGCAG AATTGTTGGT
    CCTCAAGTAG TCATATTTTG TATGCACCAC CAGCGATGTG TCCCAAGAGC CGAACATCCA
    GTTTATAATA TGGTTATGTC TGATGTAACC ATATCTTGTA CAAGTCTGGA AAAAGAAAAA
    AGGGAAGAAG TTCATAAATA TGTACAAATG ATGGGCGGAC GAGTATACAG AGACCTTAAT
    GTATCAGTAA CTCACCTTAT TGCAGGAGAA GTTGGTAGCA AAAAATATTT AGTTGCTGCA
    AACCTGAAGA AACCTATTTT GCTTCCCTCT TGGATAAAAA CACTTTGGGA GAAGTCACAA
    GAGAAAAAAA TAACTAGATA TACTGATATA AACATGGAAG ATTTCAAGTG TCCTATTTTT
    CTTGGTTGCA TAATCTGTGT GACTGGCTTA TGTGGCTTAG ACAGGAAAGA AGTTCAGCAA
    CTCACAGTTA AGCATGGAGG TCAATACATG GGACAATTGA AAATGAATGA ATGTACACAC
    CTCATTGTGC AAGAACCAAA AGGTCAGAAG TATGAGTGTG CCAAGAGATG GAATGTACAC
    TGTGTGACCA CACAGTGGTT TTTTGACAGT ATTGAGAAAG GTTTTTGTCA GGATGAATCC
    ATATACAAGA CAGAACCTAG ACCAGAAGCA AAGACTATGC CCAATTCTTC AACTCCTACC
    AGCCAGATCA ACACAATTGA TAGTCGTACT CTTTCAGATG TCAGCAATAT TTCCAACATA
    AATGCAAGTT GCGTAAGTGA ATCAATATGT AATTCACTTA ACAGCAAACT GGAGCCTACA
    CTTGAAAATC TAGAAAATCT GGATGTCAGT GCATTTCAAG CACCTGAAGA TTTATTAGAT
    GGTTGTCGGA TATATCTTTG CGGTTTTAGT GGCAGAAAGC TAGATAAACT GAGAAGACTT
    ATTAACAGTG GAGGTGGAGT TCGTTTTAAC CAGCTAAATG AAGATGTAAC TCATGTTATT
    GTGGGAGATT ATGATGATGA ATTGAAGCAG TTTTGGAATA AATCAGCCCA CAGGCCTCAT
    GTAGTGGGAG CAAAGTGGTT GCTAGAGTGT TTCAGTAAAG GTTATATGCT TTCTGAAGAA
    CCATATATCC ATGCTAATTA CCAGCCAGTG GAAATTCCAG TTTCACATAA GCCTGAAAGT
    AAAGCAGCTC TTTTAAAAAA GAAGAACAGC AGCTTCTCTA AGAAAGACTT TGCTCCTAGT
    GAAAAGCATG AGCAAGCTGA TGAAGATCTG CTCTCTCAAT ATGAAAATGG TAGCTCCACA
    GTAGTTGAGG CTAAGACGTC TGAAGCCAGG CCCTTTAATG ATTCTACTCA TGCTGAGCCC
    TTGAATGATT CTACTCACAT TTCTTTGCAA GAAGAAAACC AGTCTTCTGT CAGTCATTGT
    GTCCCTGATG TTTCTACAAT TACTGAAGAA GGCTTATTTA GCCAAAAGAG TTTCCTTGTT
    TTGGGTTTTA GTAATGAAAA TGAATCTAAC ATCGCAAACA TCATAAAAGA AAATGCTGGG
    AAAATCATGT CCCTTCTGAG CAGAACTGTT GCGGATTATG CTGTGGTTCC TCTGCTGGGG
    TGTGAAGTGG AAGCCACTGT GGGAGAAGTT GTTACAAATA CATGGCTGGT TACTTGCATA
    GACTATCAGA CTTTGTTTGA TCCAAAGTCG AATCCTCTCT TCACACCAGT TCCAGTAATG
    ACAGGAATGA CTCCTTTAGA GGATTGTGTT ATTTCATTTA GCCAGTGTGC TGGAGCAGAA
    AAAGAGTCTT TAACATTCCT AGCAAACCTC CTTGGAGCAA GTGTTCAAGA ATACTTTGTT
    CGCAAATCCA ATGCAAAGAA AGGCATGTTT GCCAGTACTC ATCTTATACT GAAAGAACGT
    GGTGGCTCTA AATATGAAGC TGCAAAGAAG TGGAATTTAC CTGCCGTTAC TATAGCTTGG
    CTGTTGGAGA CTGCTAGAAC GGGAAAGAGA GCAGACGAAA GCCATTTTCT GATTGAAAAT
    TCAACTAAAG AAGAACGAAG TTTGGAAACA GAAATAACAA ATGGAATCAA TCTAAATTCA
    GATACTGCAG AGCATCCTGG CACACGCCTG CAAACTCACA GAAAAACCGT CGTTACACCT
    TTAGATATGA ACCGCTTTCA GAGTAAAGCT TTCCGTGCTG TGGTCTCACA ACATGCCAGA
    CAGGTCGCAG CCTCCCCAGC AGTAGGACAA CCACTTCAGA AGGAGCCCTC GTTACACCTG
    GATACACCAT CAAAATTCCT GTCCAAGGAC AAACTCTTCA AGCCTTCCTT TGATGTGAAG
    GATGCACTTG CAGCCTTGGA AACTCCAGGA CGTCCCAGCC AACAGAAAAG GAAACCGAGT
    ACGCCACTCT CAGAAGTTAT TGTCAAAAAC TTGCAACTTG CTTTGGCAAA TAGCTCTCGA
    AATGCTGTCG CTCTTTCTGC CAGCCCTCAA CTGAAAGAGG CCCAGTCAGA GAAGGAAGAA
    GCCCCAAAGC CACTTCACAA AGTAGTGGTA TGTGTTAGTA AAAAACTCAG TAAGAAGCAG
    AGTGAACTAA ATGGGATCGC AGCCTCTCTA GGAGCAGATT ACAGGTGGAG TTTTGATGAA
    ACAGTGACTC ATTTCATCTA TCAAGGGCGG CCAAATGACA CTAATCGGGA GTATAAATCT
    GTAAAAGAAA GAGGAGTACA CATTGTTTCC GAGCACTGGC TTTTAGATTG TGCCCAAGAG
    TGTAAACATC TTCCTGAATC TCTTTATCCA CATACTTATA ATCCCAAAAT GAGCTTGGAT
    ATCAGCGCAG TGCAAGATGG CCGGCTCTGT AATAGTCGAC TACTCTCAGC TGTGTCTTCA
    ACAAAGGATG ATGAGCCAGA TCCTTTGATT TTAGAAGAAA ATGATGTAGA CAATATGGCC
    ACCAATAATA AAGAGTCAGC ACCATCAAAT GGAAGTGGAA AGAATGACTC TAAAGGAGTT
    CTGACACAGA CCTTAGAGAT GAGAGAGAAC TTTCAGAAGC AGTTACAGGA GATAATGTCT
    GCAACATCAA TAGTGAAACC CCAAGGGCAG AGGACTTCCC TTTCAAGAAG TGGTTGTAAC
    AGCGCATCTT CAACCCCTGA CAGCACTCGC TCTGCTCGCA GTGGACGAAG TAGAGTCCTA
    GAGGCACTGA GGCAGTCTCG TCAGACAGTA CCTGATGTCA ACACAGAGCC TTCCCAAAAT
    GAACAGATCA TTTGGGATGA CCCTACAGCA AGGGAGGAGA GAGCAAGGCT TGCCAGCAAT
    TTGCAGTGGC CTAGTTGTCC CACACAATAC TCTGAGCTTC AGGTTGACAT TCAAAACTTG
    GAGGATTCTC CTTTTCAAAA GCCTTTACAT GATTCAGAAA TTGCTAAACA GGCTGTCTGT
    GATCCTGGAA ACATACGTGT GACTGAAGCT CCCAAACACC CAATCTCTGA AGAACTGGAA
    ACTCCCATAA AAGACAGCCA CCTGATCCCT ACGCCTCAAG CCCCCAGTAT TGCCTTTCCA
    CTCGCCAACC CCCCTGTGGC TCCGCACCCT AGAGAAAAGA TTATAACGAT AGAGGAGACT
    CATGAAGAAT TAAAAAAACA GTACATATTT CAGTTATCAT CTCTGAATCC TCAAGAACGT
    ATTGACTATT GTCATCTGAT TGAGAAACTA GGTGGATTGG TGATAGAAAA GCAGTGCTTT
    GATCCCACCT GTACACACAT TGTTGTGGGA CATCCACTTC GAAACGAGAA GTATTTAGCC
    TCAGTGGCAG CTGGGAAGTG GGTGCTTCAT CGCTCCTACC TTGAAGCCTG CAGGACTGCT
    GGACACTTCG TGCAGGAAGA AGACTATGAA TGGGGAAGTA GTTCCATACT TGATGTTCTG
    ACTGGAATCA ATGTACAGCA ACGAAGACTA GCACTTGCAG CAATGAGATG GAGAAAAAAA
    ATCCAGCAAA GACAAGAATC TGGCATTGTT GAGGGAGCAT TTAGTGGGTG GAAGGTTATT
    TTACATGTGG ATCAGTCTCG AGAAGCAGGC TTCAAACGCC TTCTTCAGTC AGGAGGAGCA
    AAGGTGCTAC CTGGTCATTC TGTACCTTTA TTTAAAGAGG CCACACATCT TTTTTCTGAC
    TTGAATAAAC TGAAACCAGA TGACTCAGGA GTTAATATAG CAGAAGCTGC TGCCCAGAAC
    GTGTACTGCT TGAGAACAGA ATACATTGCT GATTATCTCA TGCAGGAATC ACCTCCTCAT
    GTAGAAAATT ACTGTCTACC AGAAGCTATT TCATTTATTC AGAATAATAA GGAACTTGGG
    ACTGGATTAT CACAAAAGAG GAAAGCTCCT ACAGAAAAAA ATAAAATCAA ACGACCTAGA
    GTACACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu16438
    Clone ID Related Accession (Same CDS sequence) XM_017005636.1 , XM_017005636.2 , NM_007027.3 , NM_007027.4
    Accession Version NM_007027.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4569bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-15
    Organism Homo sapiens(human)
    Product DNA topoisomerase 2-binding protein 1 isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC083905.20, BC032633.1, BC126209.1, BC151237.1 and AC016255.24. On May 17, 2019 this sequence version replaced NM_007027.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.145815.1, SRR1660803.23079.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000260810.10/ ENSP00000260810.5 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGTCCAGAA ATGACAAAGA ACCGTTTTTT GTGAAGTTTT TAAAGTCTTC AGACAATTCC 
    AAATGTTTTT TTAAAGCTCT CGAGTCCATA AAAGAATTCC AATCAGAAGA ATATCTTCAG
    ATTATTACAG AAGAAGAGGC ATTGAAGATA AAGGAGAATG ATAGATCACT TTATATCTGT
    GACCCTTTTA GTGGCGTTGT CTTTGATCAC CTCAAAAAGC TTGGCTGCAG AATTGTTGGT
    CCTCAAGTAG TCATATTTTG TATGCACCAC CAGCGATGTG TCCCAAGAGC CGAACATCCA
    GTTTATAATA TGGTTATGTC TGATGTAACC ATATCTTGTA CAAGTCTGGA AAAAGAAAAA
    AGGGAAGAAG TTCATAAATA TGTACAAATG ATGGGCGGAC GAGTATACAG AGACCTTAAT
    GTATCAGTAA CTCACCTTAT TGCAGGAGAA GTTGGTAGCA AAAAATATTT AGTTGCTGCA
    AACCTGAAGA AACCTATTTT GCTTCCCTCT TGGATAAAAA CACTTTGGGA GAAGTCACAA
    GAGAAAAAAA TAACTAGATA TACTGATATA AACATGGAAG ATTTCAAGTG TCCTATTTTT
    CTTGGTTGCA TAATCTGTGT GACTGGCTTA TGTGGCTTAG ACAGGAAAGA AGTTCAGCAA
    CTCACAGTTA AGCATGGAGG TCAATACATG GGACAATTGA AAATGAATGA ATGTACACAC
    CTCATTGTGC AAGAACCAAA AGGTCAGAAG TATGAGTGTG CCAAGAGATG GAATGTACAC
    TGTGTGACCA CACAGTGGTT TTTTGACAGT ATTGAGAAAG GTTTTTGTCA GGATGAATCC
    ATATACAAGA CAGAACCTAG ACCAGAAGCA AAGACTATGC CCAATTCTTC AACTCCTACC
    AGCCAGATCA ACACAATTGA TAGTCGTACT CTTTCAGATG TCAGCAATAT TTCCAACATA
    AATGCAAGTT GCGTAAGTGA ATCAATATGT AATTCACTTA ACAGCAAACT GGAGCCTACA
    CTTGAAAATC TAGAAAATCT GGATGTCAGT GCATTTCAAG CACCTGAAGA TTTATTAGAT
    GGTTGTCGGA TATATCTTTG CGGTTTTAGT GGCAGAAAGC TAGATAAACT GAGAAGACTT
    ATTAACAGTG GAGGTGGAGT TCGTTTTAAC CAGCTAAATG AAGATGTAAC TCATGTTATT
    GTGGGAGATT ATGATGATGA ATTGAAGCAG TTTTGGAATA AATCAGCCCA CAGGCCTCAT
    GTAGTGGGAG CAAAGTGGTT GCTAGAGTGT TTCAGTAAAG GTTATATGCT TTCTGAAGAA
    CCATATATCC ATGCTAATTA CCAGCCAGTG GAAATTCCAG TTTCACATAA GCCTGAAAGT
    AAAGCAGCTC TTTTAAAAAA GAAGAACAGC AGCTTCTCTA AGAAAGACTT TGCTCCTAGT
    GAAAAGCATG AGCAAGCTGA TGAAGATCTG CTCTCTCAAT ATGAAAATGG TAGCTCCACA
    GTAGTTGAGG CTAAGACGTC TGAAGCCAGG CCCTTTAATG ATTCTACTCA TGCTGAGCCC
    TTGAATGATT CTACTCACAT TTCTTTGCAA GAAGAAAACC AGTCTTCTGT CAGTCATTGT
    GTCCCTGATG TTTCTACAAT TACTGAAGAA GGCTTATTTA GCCAAAAGAG TTTCCTTGTT
    TTGGGTTTTA GTAATGAAAA TGAATCTAAC ATCGCAAACA TCATAAAAGA AAATGCTGGG
    AAAATCATGT CCCTTCTGAG CAGAACTGTT GCGGATTATG CTGTGGTTCC TCTGCTGGGG
    TGTGAAGTGG AAGCCACTGT GGGAGAAGTT GTTACAAATA CATGGCTGGT TACTTGCATA
    GACTATCAGA CTTTGTTTGA TCCAAAGTCG AATCCTCTCT TCACACCAGT TCCAGTAATG
    ACAGGAATGA CTCCTTTAGA GGATTGTGTT ATTTCATTTA GCCAGTGTGC TGGAGCAGAA
    AAAGAGTCTT TAACATTCCT AGCAAACCTC CTTGGAGCAA GTGTTCAAGA ATACTTTGTT
    CGCAAATCCA ATGCAAAGAA AGGCATGTTT GCCAGTACTC ATCTTATACT GAAAGAACGT
    GGTGGCTCTA AATATGAAGC TGCAAAGAAG TGGAATTTAC CTGCCGTTAC TATAGCTTGG
    CTGTTGGAGA CTGCTAGAAC GGGAAAGAGA GCAGACGAAA GCCATTTTCT GATTGAAAAT
    TCAACTAAAG AAGAACGAAG TTTGGAAACA GAAATAACAA ATGGAATCAA TCTAAATTCA
    GATACTGCAG AGCATCCTGG CACACGCCTG CAAACTCACA GAAAAACCGT CGTTACACCT
    TTAGATATGA ACCGCTTTCA GAGTAAAGCT TTCCGTGCTG TGGTCTCACA ACATGCCAGA
    CAGGTCGCAG CCTCCCCAGC AGTAGGACAA CCACTTCAGA AGGAGCCCTC GTTACACCTG
    GATACACCAT CAAAATTCCT GTCCAAGGAC AAACTCTTCA AGCCTTCCTT TGATGTGAAG
    GATGCACTTG CAGCCTTGGA AACTCCAGGA CGTCCCAGCC AACAGAAAAG GAAACCGAGT
    ACGCCACTCT CAGAAGTTAT TGTCAAAAAC TTGCAACTTG CTTTGGCAAA TAGCTCTCGA
    AATGCTGTCG CTCTTTCTGC CAGCCCTCAA CTGAAAGAGG CCCAGTCAGA GAAGGAAGAA
    GCCCCAAAGC CACTTCACAA AGTAGTGGTA TGTGTTAGTA AAAAACTCAG TAAGAAGCAG
    AGTGAACTAA ATGGGATCGC AGCCTCTCTA GGAGCAGATT ACAGGTGGAG TTTTGATGAA
    ACAGTGACTC ATTTCATCTA TCAAGGGCGG CCAAATGACA CTAATCGGGA GTATAAATCT
    GTAAAAGAAA GAGGAGTACA CATTGTTTCC GAGCACTGGC TTTTAGATTG TGCCCAAGAG
    TGTAAACATC TTCCTGAATC TCTTTATCCA CATACTTATA ATCCCAAAAT GAGCTTGGAT
    ATCAGCGCAG TGCAAGATGG CCGGCTCTGT AATAGTCGAC TACTCTCAGC TGTGTCTTCA
    ACAAAGGATG ATGAGCCAGA TCCTTTGATT TTAGAAGAAA ATGATGTAGA CAATATGGCC
    ACCAATAATA AAGAGTCAGC ACCATCAAAT GGAAGTGGAA AGAATGACTC TAAAGGAGTT
    CTGACACAGA CCTTAGAGAT GAGAGAGAAC TTTCAGAAGC AGTTACAGGA GATAATGTCT
    GCAACATCAA TAGTGAAACC CCAAGGGCAG AGGACTTCCC TTTCAAGAAG TGGTTGTAAC
    AGCGCATCTT CAACCCCTGA CAGCACTCGC TCTGCTCGCA GTGGACGAAG TAGAGTCCTA
    GAGGCACTGA GGCAGTCTCG TCAGACAGTA CCTGATGTCA ACACAGAGCC TTCCCAAAAT
    GAACAGATCA TTTGGGATGA CCCTACAGCA AGGGAGGAGA GAGCAAGGCT TGCCAGCAAT
    TTGCAGTGGC CTAGTTGTCC CACACAATAC TCTGAGCTTC AGGTTGACAT TCAAAACTTG
    GAGGATTCTC CTTTTCAAAA GCCTTTACAT GATTCAGAAA TTGCTAAACA GGCTGTCTGT
    GATCCTGGAA ACATACGTGT GACTGAAGCT CCCAAACACC CAATCTCTGA AGAACTGGAA
    ACTCCCATAA AAGACAGCCA CCTGATCCCT ACGCCTCAAG CCCCCAGTAT TGCCTTTCCA
    CTCGCCAACC CCCCTGTGGC TCCGCACCCT AGAGAAAAGA TTATAACGAT AGAGGAGACT
    CATGAAGAAT TAAAAAAACA GTACATATTT CAGTTATCAT CTCTGAATCC TCAAGAACGT
    ATTGACTATT GTCATCTGAT TGAGAAACTA GGTGGATTGG TGATAGAAAA GCAGTGCTTT
    GATCCCACCT GTACACACAT TGTTGTGGGA CATCCACTTC GAAACGAGAA GTATTTAGCC
    TCAGTGGCAG CTGGGAAGTG GGTGCTTCAT CGCTCCTACC TTGAAGCCTG CAGGACTGCT
    GGACACTTCG TGCAGGAAGA AGACTATGAA TGGGGAAGTA GTTCCATACT TGATGTTCTG
    ACTGGAATCA ATGTACAGCA ACGAAGACTA GCACTTGCAG CAATGAGATG GAGAAAAAAA
    ATCCAGCAAA GACAAGAATC TGGCATTGTT GAGGGAGCAT TTAGTGGGTG GAAGGTTATT
    TTACATGTGG ATCAGTCTCG AGAAGCAGGC TTCAAACGCC TTCTTCAGTC AGGAGGAGCA
    AAGGTGCTAC CTGGTCATTC TGTACCTTTA TTTAAAGAGG CCACACATCT TTTTTCTGAC
    TTGAATAAAC TGAAACCAGA TGACTCAGGA GTTAATATAG CAGAAGCTGC TGCCCAGAAC
    GTGTACTGCT TGAGAACAGA ATACATTGCT GATTATCTCA TGCAGGAATC ACCTCCTCAT
    GTAGAAAATT ACTGTCTACC AGAAGCTATT TCATTTATTC AGAATAATAA GGAACTTGGG
    ACTGGATTAT CACAAAAGAG GAAAGCTCCT ACAGAAAAAA ATAAAATCAA ACGACCTAGA
    GTACACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_008958.2
    CDS181..4749
    Translation

    Target ORF information:

    RefSeq Version NM_007027.4
    Organism Homo sapiens(human)
    Definition Homo sapiens DNA topoisomerase II binding protein 1 (TOPBP1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_007027.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGTCCAGAA ATGACAAAGA ACCGTTTTTT GTGAAGTTTT TAAAGTCTTC AGACAATTCC 
    AAATGTTTTT TTAAAGCTCT CGAGTCCATA AAAGAATTCC AATCAGAAGA ATATCTTCAG
    ATTATTACAG AAGAAGAGGC ATTGAAGATA AAGGAGAATG ATAGATCACT TTATATCTGT
    GACCCTTTTA GTGGCGTTGT CTTTGATCAC CTCAAAAAGC TTGGCTGCAG AATTGTTGGT
    CCTCAAGTAG TCATATTTTG TATGCACCAC CAGCGATGTG TCCCAAGAGC CGAACATCCA
    GTTTATAATA TGGTTATGTC TGATGTAACC ATATCTTGTA CAAGTCTGGA AAAAGAAAAA
    AGGGAAGAAG TTCATAAATA TGTACAAATG ATGGGCGGAC GAGTATACAG AGACCTTAAT
    GTATCAGTAA CTCACCTTAT TGCAGGAGAA GTTGGTAGCA AAAAATATTT AGTTGCTGCA
    AACCTGAAGA AACCTATTTT GCTTCCCTCT TGGATAAAAA CACTTTGGGA GAAGTCACAA
    GAGAAAAAAA TAACTAGATA TACTGATATA AACATGGAAG ATTTCAAGTG TCCTATTTTT
    CTTGGTTGCA TAATCTGTGT GACTGGCTTA TGTGGCTTAG ACAGGAAAGA AGTTCAGCAA
    CTCACAGTTA AGCATGGAGG TCAATACATG GGACAATTGA AAATGAATGA ATGTACACAC
    CTCATTGTGC AAGAACCAAA AGGTCAGAAG TATGAGTGTG CCAAGAGATG GAATGTACAC
    TGTGTGACCA CACAGTGGTT TTTTGACAGT ATTGAGAAAG GTTTTTGTCA GGATGAATCC
    ATATACAAGA CAGAACCTAG ACCAGAAGCA AAGACTATGC CCAATTCTTC AACTCCTACC
    AGCCAGATCA ACACAATTGA TAGTCGTACT CTTTCAGATG TCAGCAATAT TTCCAACATA
    AATGCAAGTT GCGTAAGTGA ATCAATATGT AATTCACTTA ACAGCAAACT GGAGCCTACA
    CTTGAAAATC TAGAAAATCT GGATGTCAGT GCATTTCAAG CACCTGAAGA TTTATTAGAT
    GGTTGTCGGA TATATCTTTG CGGTTTTAGT GGCAGAAAGC TAGATAAACT GAGAAGACTT
    ATTAACAGTG GAGGTGGAGT TCGTTTTAAC CAGCTAAATG AAGATGTAAC TCATGTTATT
    GTGGGAGATT ATGATGATGA ATTGAAGCAG TTTTGGAATA AATCAGCCCA CAGGCCTCAT
    GTAGTGGGAG CAAAGTGGTT GCTAGAGTGT TTCAGTAAAG GTTATATGCT TTCTGAAGAA
    CCATATATCC ATGCTAATTA CCAGCCAGTG GAAATTCCAG TTTCACATAA GCCTGAAAGT
    AAAGCAGCTC TTTTAAAAAA GAAGAACAGC AGCTTCTCTA AGAAAGACTT TGCTCCTAGT
    GAAAAGCATG AGCAAGCTGA TGAAGATCTG CTCTCTCAAT ATGAAAATGG TAGCTCCACA
    GTAGTTGAGG CTAAGACGTC TGAAGCCAGG CCCTTTAATG ATTCTACTCA TGCTGAGCCC
    TTGAATGATT CTACTCACAT TTCTTTGCAA GAAGAAAACC AGTCTTCTGT CAGTCATTGT
    GTCCCTGATG TTTCTACAAT TACTGAAGAA GGCTTATTTA GCCAAAAGAG TTTCCTTGTT
    TTGGGTTTTA GTAATGAAAA TGAATCTAAC ATCGCAAACA TCATAAAAGA AAATGCTGGG
    AAAATCATGT CCCTTCTGAG CAGAACTGTT GCGGATTATG CTGTGGTTCC TCTGCTGGGG
    TGTGAAGTGG AAGCCACTGT GGGAGAAGTT GTTACAAATA CATGGCTGGT TACTTGCATA
    GACTATCAGA CTTTGTTTGA TCCAAAGTCG AATCCTCTCT TCACACCAGT TCCAGTAATG
    ACAGGAATGA CTCCTTTAGA GGATTGTGTT ATTTCATTTA GCCAGTGTGC TGGAGCAGAA
    AAAGAGTCTT TAACATTCCT AGCAAACCTC CTTGGAGCAA GTGTTCAAGA ATACTTTGTT
    CGCAAATCCA ATGCAAAGAA AGGCATGTTT GCCAGTACTC ATCTTATACT GAAAGAACGT
    GGTGGCTCTA AATATGAAGC TGCAAAGAAG TGGAATTTAC CTGCCGTTAC TATAGCTTGG
    CTGTTGGAGA CTGCTAGAAC GGGAAAGAGA GCAGACGAAA GCCATTTTCT GATTGAAAAT
    TCAACTAAAG AAGAACGAAG TTTGGAAACA GAAATAACAA ATGGAATCAA TCTAAATTCA
    GATACTGCAG AGCATCCTGG CACACGCCTG CAAACTCACA GAAAAACCGT CGTTACACCT
    TTAGATATGA ACCGCTTTCA GAGTAAAGCT TTCCGTGCTG TGGTCTCACA ACATGCCAGA
    CAGGTCGCAG CCTCCCCAGC AGTAGGACAA CCACTTCAGA AGGAGCCCTC GTTACACCTG
    GATACACCAT CAAAATTCCT GTCCAAGGAC AAACTCTTCA AGCCTTCCTT TGATGTGAAG
    GATGCACTTG CAGCCTTGGA AACTCCAGGA CGTCCCAGCC AACAGAAAAG GAAACCGAGT
    ACGCCACTCT CAGAAGTTAT TGTCAAAAAC TTGCAACTTG CTTTGGCAAA TAGCTCTCGA
    AATGCTGTCG CTCTTTCTGC CAGCCCTCAA CTGAAAGAGG CCCAGTCAGA GAAGGAAGAA
    GCCCCAAAGC CACTTCACAA AGTAGTGGTA TGTGTTAGTA AAAAACTCAG TAAGAAGCAG
    AGTGAACTAA ATGGGATCGC AGCCTCTCTA GGAGCAGATT ACAGGTGGAG TTTTGATGAA
    ACAGTGACTC ATTTCATCTA TCAAGGGCGG CCAAATGACA CTAATCGGGA GTATAAATCT
    GTAAAAGAAA GAGGAGTACA CATTGTTTCC GAGCACTGGC TTTTAGATTG TGCCCAAGAG
    TGTAAACATC TTCCTGAATC TCTTTATCCA CATACTTATA ATCCCAAAAT GAGCTTGGAT
    ATCAGCGCAG TGCAAGATGG CCGGCTCTGT AATAGTCGAC TACTCTCAGC TGTGTCTTCA
    ACAAAGGATG ATGAGCCAGA TCCTTTGATT TTAGAAGAAA ATGATGTAGA CAATATGGCC
    ACCAATAATA AAGAGTCAGC ACCATCAAAT GGAAGTGGAA AGAATGACTC TAAAGGAGTT
    CTGACACAGA CCTTAGAGAT GAGAGAGAAC TTTCAGAAGC AGTTACAGGA GATAATGTCT
    GCAACATCAA TAGTGAAACC CCAAGGGCAG AGGACTTCCC TTTCAAGAAG TGGTTGTAAC
    AGCGCATCTT CAACCCCTGA CAGCACTCGC TCTGCTCGCA GTGGACGAAG TAGAGTCCTA
    GAGGCACTGA GGCAGTCTCG TCAGACAGTA CCTGATGTCA ACACAGAGCC TTCCCAAAAT
    GAACAGATCA TTTGGGATGA CCCTACAGCA AGGGAGGAGA GAGCAAGGCT TGCCAGCAAT
    TTGCAGTGGC CTAGTTGTCC CACACAATAC TCTGAGCTTC AGGTTGACAT TCAAAACTTG
    GAGGATTCTC CTTTTCAAAA GCCTTTACAT GATTCAGAAA TTGCTAAACA GGCTGTCTGT
    GATCCTGGAA ACATACGTGT GACTGAAGCT CCCAAACACC CAATCTCTGA AGAACTGGAA
    ACTCCCATAA AAGACAGCCA CCTGATCCCT ACGCCTCAAG CCCCCAGTAT TGCCTTTCCA
    CTCGCCAACC CCCCTGTGGC TCCGCACCCT AGAGAAAAGA TTATAACGAT AGAGGAGACT
    CATGAAGAAT TAAAAAAACA GTACATATTT CAGTTATCAT CTCTGAATCC TCAAGAACGT
    ATTGACTATT GTCATCTGAT TGAGAAACTA GGTGGATTGG TGATAGAAAA GCAGTGCTTT
    GATCCCACCT GTACACACAT TGTTGTGGGA CATCCACTTC GAAACGAGAA GTATTTAGCC
    TCAGTGGCAG CTGGGAAGTG GGTGCTTCAT CGCTCCTACC TTGAAGCCTG CAGGACTGCT
    GGACACTTCG TGCAGGAAGA AGACTATGAA TGGGGAAGTA GTTCCATACT TGATGTTCTG
    ACTGGAATCA ATGTACAGCA ACGAAGACTA GCACTTGCAG CAATGAGATG GAGAAAAAAA
    ATCCAGCAAA GACAAGAATC TGGCATTGTT GAGGGAGCAT TTAGTGGGTG GAAGGTTATT
    TTACATGTGG ATCAGTCTCG AGAAGCAGGC TTCAAACGCC TTCTTCAGTC AGGAGGAGCA
    AAGGTGCTAC CTGGTCATTC TGTACCTTTA TTTAAAGAGG CCACACATCT TTTTTCTGAC
    TTGAATAAAC TGAAACCAGA TGACTCAGGA GTTAATATAG CAGAAGCTGC TGCCCAGAAC
    GTGTACTGCT TGAGAACAGA ATACATTGCT GATTATCTCA TGCAGGAATC ACCTCCTCAT
    GTAGAAAATT ACTGTCTACC AGAAGCTATT TCATTTATTC AGAATAATAA GGAACTTGGG
    ACTGGATTAT CACAAAAGAG GAAAGCTCCT ACAGAAAAAA ATAAAATCAA ACGACCTAGA
    GTACACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu16438
    Clone ID Related Accession (Same CDS sequence) XM_017005636.1 , XM_017005636.2 , NM_007027.3 , NM_007027.4
    Accession Version XM_017005636.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4569bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product DNA topoisomerase 2-binding protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGTCCAGAA ATGACAAAGA ACCGTTTTTT GTGAAGTTTT TAAAGTCTTC AGACAATTCC 
    AAATGTTTTT TTAAAGCTCT CGAGTCCATA AAAGAATTCC AATCAGAAGA ATATCTTCAG
    ATTATTACAG AAGAAGAGGC ATTGAAGATA AAGGAGAATG ATAGATCACT TTATATCTGT
    GACCCTTTTA GTGGCGTTGT CTTTGATCAC CTCAAAAAGC TTGGCTGCAG AATTGTTGGT
    CCTCAAGTAG TCATATTTTG TATGCACCAC CAGCGATGTG TCCCAAGAGC CGAACATCCA
    GTTTATAATA TGGTTATGTC TGATGTAACC ATATCTTGTA CAAGTCTGGA AAAAGAAAAA
    AGGGAAGAAG TTCATAAATA TGTACAAATG ATGGGCGGAC GAGTATACAG AGACCTTAAT
    GTATCAGTAA CTCACCTTAT TGCAGGAGAA GTTGGTAGCA AAAAATATTT AGTTGCTGCA
    AACCTGAAGA AACCTATTTT GCTTCCCTCT TGGATAAAAA CACTTTGGGA GAAGTCACAA
    GAGAAAAAAA TAACTAGATA TACTGATATA AACATGGAAG ATTTCAAGTG TCCTATTTTT
    CTTGGTTGCA TAATCTGTGT GACTGGCTTA TGTGGCTTAG ACAGGAAAGA AGTTCAGCAA
    CTCACAGTTA AGCATGGAGG TCAATACATG GGACAATTGA AAATGAATGA ATGTACACAC
    CTCATTGTGC AAGAACCAAA AGGTCAGAAG TATGAGTGTG CCAAGAGATG GAATGTACAC
    TGTGTGACCA CACAGTGGTT TTTTGACAGT ATTGAGAAAG GTTTTTGTCA GGATGAATCC
    ATATACAAGA CAGAACCTAG ACCAGAAGCA AAGACTATGC CCAATTCTTC AACTCCTACC
    AGCCAGATCA ACACAATTGA TAGTCGTACT CTTTCAGATG TCAGCAATAT TTCCAACATA
    AATGCAAGTT GCGTAAGTGA ATCAATATGT AATTCACTTA ACAGCAAACT GGAGCCTACA
    CTTGAAAATC TAGAAAATCT GGATGTCAGT GCATTTCAAG CACCTGAAGA TTTATTAGAT
    GGTTGTCGGA TATATCTTTG CGGTTTTAGT GGCAGAAAGC TAGATAAACT GAGAAGACTT
    ATTAACAGTG GAGGTGGAGT TCGTTTTAAC CAGCTAAATG AAGATGTAAC TCATGTTATT
    GTGGGAGATT ATGATGATGA ATTGAAGCAG TTTTGGAATA AATCAGCCCA CAGGCCTCAT
    GTAGTGGGAG CAAAGTGGTT GCTAGAGTGT TTCAGTAAAG GTTATATGCT TTCTGAAGAA
    CCATATATCC ATGCTAATTA CCAGCCAGTG GAAATTCCAG TTTCACATAA GCCTGAAAGT
    AAAGCAGCTC TTTTAAAAAA GAAGAACAGC AGCTTCTCTA AGAAAGACTT TGCTCCTAGT
    GAAAAGCATG AGCAAGCTGA TGAAGATCTG CTCTCTCAAT ATGAAAATGG TAGCTCCACA
    GTAGTTGAGG CTAAGACGTC TGAAGCCAGG CCCTTTAATG ATTCTACTCA TGCTGAGCCC
    TTGAATGATT CTACTCACAT TTCTTTGCAA GAAGAAAACC AGTCTTCTGT CAGTCATTGT
    GTCCCTGATG TTTCTACAAT TACTGAAGAA GGCTTATTTA GCCAAAAGAG TTTCCTTGTT
    TTGGGTTTTA GTAATGAAAA TGAATCTAAC ATCGCAAACA TCATAAAAGA AAATGCTGGG
    AAAATCATGT CCCTTCTGAG CAGAACTGTT GCGGATTATG CTGTGGTTCC TCTGCTGGGG
    TGTGAAGTGG AAGCCACTGT GGGAGAAGTT GTTACAAATA CATGGCTGGT TACTTGCATA
    GACTATCAGA CTTTGTTTGA TCCAAAGTCG AATCCTCTCT TCACACCAGT TCCAGTAATG
    ACAGGAATGA CTCCTTTAGA GGATTGTGTT ATTTCATTTA GCCAGTGTGC TGGAGCAGAA
    AAAGAGTCTT TAACATTCCT AGCAAACCTC CTTGGAGCAA GTGTTCAAGA ATACTTTGTT
    CGCAAATCCA ATGCAAAGAA AGGCATGTTT GCCAGTACTC ATCTTATACT GAAAGAACGT
    GGTGGCTCTA AATATGAAGC TGCAAAGAAG TGGAATTTAC CTGCCGTTAC TATAGCTTGG
    CTGTTGGAGA CTGCTAGAAC GGGAAAGAGA GCAGACGAAA GCCATTTTCT GATTGAAAAT
    TCAACTAAAG AAGAACGAAG TTTGGAAACA GAAATAACAA ATGGAATCAA TCTAAATTCA
    GATACTGCAG AGCATCCTGG CACACGCCTG CAAACTCACA GAAAAACCGT CGTTACACCT
    TTAGATATGA ACCGCTTTCA GAGTAAAGCT TTCCGTGCTG TGGTCTCACA ACATGCCAGA
    CAGGTCGCAG CCTCCCCAGC AGTAGGACAA CCACTTCAGA AGGAGCCCTC GTTACACCTG
    GATACACCAT CAAAATTCCT GTCCAAGGAC AAACTCTTCA AGCCTTCCTT TGATGTGAAG
    GATGCACTTG CAGCCTTGGA AACTCCAGGA CGTCCCAGCC AACAGAAAAG GAAACCGAGT
    ACGCCACTCT CAGAAGTTAT TGTCAAAAAC TTGCAACTTG CTTTGGCAAA TAGCTCTCGA
    AATGCTGTCG CTCTTTCTGC CAGCCCTCAA CTGAAAGAGG CCCAGTCAGA GAAGGAAGAA
    GCCCCAAAGC CACTTCACAA AGTAGTGGTA TGTGTTAGTA AAAAACTCAG TAAGAAGCAG
    AGTGAACTAA ATGGGATCGC AGCCTCTCTA GGAGCAGATT ACAGGTGGAG TTTTGATGAA
    ACAGTGACTC ATTTCATCTA TCAAGGGCGG CCAAATGACA CTAATCGGGA GTATAAATCT
    GTAAAAGAAA GAGGAGTACA CATTGTTTCC GAGCACTGGC TTTTAGATTG TGCCCAAGAG
    TGTAAACATC TTCCTGAATC TCTTTATCCA CATACTTATA ATCCCAAAAT GAGCTTGGAT
    ATCAGCGCAG TGCAAGATGG CCGGCTCTGT AATAGTCGAC TACTCTCAGC TGTGTCTTCA
    ACAAAGGATG ATGAGCCAGA TCCTTTGATT TTAGAAGAAA ATGATGTAGA CAATATGGCC
    ACCAATAATA AAGAGTCAGC ACCATCAAAT GGAAGTGGAA AGAATGACTC TAAAGGAGTT
    CTGACACAGA CCTTAGAGAT GAGAGAGAAC TTTCAGAAGC AGTTACAGGA GATAATGTCT
    GCAACATCAA TAGTGAAACC CCAAGGGCAG AGGACTTCCC TTTCAAGAAG TGGTTGTAAC
    AGCGCATCTT CAACCCCTGA CAGCACTCGC TCTGCTCGCA GTGGACGAAG TAGAGTCCTA
    GAGGCACTGA GGCAGTCTCG TCAGACAGTA CCTGATGTCA ACACAGAGCC TTCCCAAAAT
    GAACAGATCA TTTGGGATGA CCCTACAGCA AGGGAGGAGA GAGCAAGGCT TGCCAGCAAT
    TTGCAGTGGC CTAGTTGTCC CACACAATAC TCTGAGCTTC AGGTTGACAT TCAAAACTTG
    GAGGATTCTC CTTTTCAAAA GCCTTTACAT GATTCAGAAA TTGCTAAACA GGCTGTCTGT
    GATCCTGGAA ACATACGTGT GACTGAAGCT CCCAAACACC CAATCTCTGA AGAACTGGAA
    ACTCCCATAA AAGACAGCCA CCTGATCCCT ACGCCTCAAG CCCCCAGTAT TGCCTTTCCA
    CTCGCCAACC CCCCTGTGGC TCCGCACCCT AGAGAAAAGA TTATAACGAT AGAGGAGACT
    CATGAAGAAT TAAAAAAACA GTACATATTT CAGTTATCAT CTCTGAATCC TCAAGAACGT
    ATTGACTATT GTCATCTGAT TGAGAAACTA GGTGGATTGG TGATAGAAAA GCAGTGCTTT
    GATCCCACCT GTACACACAT TGTTGTGGGA CATCCACTTC GAAACGAGAA GTATTTAGCC
    TCAGTGGCAG CTGGGAAGTG GGTGCTTCAT CGCTCCTACC TTGAAGCCTG CAGGACTGCT
    GGACACTTCG TGCAGGAAGA AGACTATGAA TGGGGAAGTA GTTCCATACT TGATGTTCTG
    ACTGGAATCA ATGTACAGCA ACGAAGACTA GCACTTGCAG CAATGAGATG GAGAAAAAAA
    ATCCAGCAAA GACAAGAATC TGGCATTGTT GAGGGAGCAT TTAGTGGGTG GAAGGTTATT
    TTACATGTGG ATCAGTCTCG AGAAGCAGGC TTCAAACGCC TTCTTCAGTC AGGAGGAGCA
    AAGGTGCTAC CTGGTCATTC TGTACCTTTA TTTAAAGAGG CCACACATCT TTTTTCTGAC
    TTGAATAAAC TGAAACCAGA TGACTCAGGA GTTAATATAG CAGAAGCTGC TGCCCAGAAC
    GTGTACTGCT TGAGAACAGA ATACATTGCT GATTATCTCA TGCAGGAATC ACCTCCTCAT
    GTAGAAAATT ACTGTCTACC AGAAGCTATT TCATTTATTC AGAATAATAA GGAACTTGGG
    ACTGGATTAT CACAAAAGAG GAAAGCTCCT ACAGAAAAAA ATAAAATCAA ACGACCTAGA
    GTACACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016861125.1
    CDS203..4771
    Translation

    Target ORF information:

    RefSeq Version XM_017005636.1
    Organism Homo sapiens(human)
    Definition Homo sapiens topoisomerase (DNA) II binding protein 1 (TOPBP1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017005636.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGTCCAGAA ATGACAAAGA ACCGTTTTTT GTGAAGTTTT TAAAGTCTTC AGACAATTCC 
    AAATGTTTTT TTAAAGCTCT CGAGTCCATA AAAGAATTCC AATCAGAAGA ATATCTTCAG
    ATTATTACAG AAGAAGAGGC ATTGAAGATA AAGGAGAATG ATAGATCACT TTATATCTGT
    GACCCTTTTA GTGGCGTTGT CTTTGATCAC CTCAAAAAGC TTGGCTGCAG AATTGTTGGT
    CCTCAAGTAG TCATATTTTG TATGCACCAC CAGCGATGTG TCCCAAGAGC CGAACATCCA
    GTTTATAATA TGGTTATGTC TGATGTAACC ATATCTTGTA CAAGTCTGGA AAAAGAAAAA
    AGGGAAGAAG TTCATAAATA TGTACAAATG ATGGGCGGAC GAGTATACAG AGACCTTAAT
    GTATCAGTAA CTCACCTTAT TGCAGGAGAA GTTGGTAGCA AAAAATATTT AGTTGCTGCA
    AACCTGAAGA AACCTATTTT GCTTCCCTCT TGGATAAAAA CACTTTGGGA GAAGTCACAA
    GAGAAAAAAA TAACTAGATA TACTGATATA AACATGGAAG ATTTCAAGTG TCCTATTTTT
    CTTGGTTGCA TAATCTGTGT GACTGGCTTA TGTGGCTTAG ACAGGAAAGA AGTTCAGCAA
    CTCACAGTTA AGCATGGAGG TCAATACATG GGACAATTGA AAATGAATGA ATGTACACAC
    CTCATTGTGC AAGAACCAAA AGGTCAGAAG TATGAGTGTG CCAAGAGATG GAATGTACAC
    TGTGTGACCA CACAGTGGTT TTTTGACAGT ATTGAGAAAG GTTTTTGTCA GGATGAATCC
    ATATACAAGA CAGAACCTAG ACCAGAAGCA AAGACTATGC CCAATTCTTC AACTCCTACC
    AGCCAGATCA ACACAATTGA TAGTCGTACT CTTTCAGATG TCAGCAATAT TTCCAACATA
    AATGCAAGTT GCGTAAGTGA ATCAATATGT AATTCACTTA ACAGCAAACT GGAGCCTACA
    CTTGAAAATC TAGAAAATCT GGATGTCAGT GCATTTCAAG CACCTGAAGA TTTATTAGAT
    GGTTGTCGGA TATATCTTTG CGGTTTTAGT GGCAGAAAGC TAGATAAACT GAGAAGACTT
    ATTAACAGTG GAGGTGGAGT TCGTTTTAAC CAGCTAAATG AAGATGTAAC TCATGTTATT
    GTGGGAGATT ATGATGATGA ATTGAAGCAG TTTTGGAATA AATCAGCCCA CAGGCCTCAT
    GTAGTGGGAG CAAAGTGGTT GCTAGAGTGT TTCAGTAAAG GTTATATGCT TTCTGAAGAA
    CCATATATCC ATGCTAATTA CCAGCCAGTG GAAATTCCAG TTTCACATAA GCCTGAAAGT
    AAAGCAGCTC TTTTAAAAAA GAAGAACAGC AGCTTCTCTA AGAAAGACTT TGCTCCTAGT
    GAAAAGCATG AGCAAGCTGA TGAAGATCTG CTCTCTCAAT ATGAAAATGG TAGCTCCACA
    GTAGTTGAGG CTAAGACGTC TGAAGCCAGG CCCTTTAATG ATTCTACTCA TGCTGAGCCC
    TTGAATGATT CTACTCACAT TTCTTTGCAA GAAGAAAACC AGTCTTCTGT CAGTCATTGT
    GTCCCTGATG TTTCTACAAT TACTGAAGAA GGCTTATTTA GCCAAAAGAG TTTCCTTGTT
    TTGGGTTTTA GTAATGAAAA TGAATCTAAC ATCGCAAACA TCATAAAAGA AAATGCTGGG
    AAAATCATGT CCCTTCTGAG CAGAACTGTT GCGGATTATG CTGTGGTTCC TCTGCTGGGG
    TGTGAAGTGG AAGCCACTGT GGGAGAAGTT GTTACAAATA CATGGCTGGT TACTTGCATA
    GACTATCAGA CTTTGTTTGA TCCAAAGTCG AATCCTCTCT TCACACCAGT TCCAGTAATG
    ACAGGAATGA CTCCTTTAGA GGATTGTGTT ATTTCATTTA GCCAGTGTGC TGGAGCAGAA
    AAAGAGTCTT TAACATTCCT AGCAAACCTC CTTGGAGCAA GTGTTCAAGA ATACTTTGTT
    CGCAAATCCA ATGCAAAGAA AGGCATGTTT GCCAGTACTC ATCTTATACT GAAAGAACGT
    GGTGGCTCTA AATATGAAGC TGCAAAGAAG TGGAATTTAC CTGCCGTTAC TATAGCTTGG
    CTGTTGGAGA CTGCTAGAAC GGGAAAGAGA GCAGACGAAA GCCATTTTCT GATTGAAAAT
    TCAACTAAAG AAGAACGAAG TTTGGAAACA GAAATAACAA ATGGAATCAA TCTAAATTCA
    GATACTGCAG AGCATCCTGG CACACGCCTG CAAACTCACA GAAAAACCGT CGTTACACCT
    TTAGATATGA ACCGCTTTCA GAGTAAAGCT TTCCGTGCTG TGGTCTCACA ACATGCCAGA
    CAGGTCGCAG CCTCCCCAGC AGTAGGACAA CCACTTCAGA AGGAGCCCTC GTTACACCTG
    GATACACCAT CAAAATTCCT GTCCAAGGAC AAACTCTTCA AGCCTTCCTT TGATGTGAAG
    GATGCACTTG CAGCCTTGGA AACTCCAGGA CGTCCCAGCC AACAGAAAAG GAAACCGAGT
    ACGCCACTCT CAGAAGTTAT TGTCAAAAAC TTGCAACTTG CTTTGGCAAA TAGCTCTCGA
    AATGCTGTCG CTCTTTCTGC CAGCCCTCAA CTGAAAGAGG CCCAGTCAGA GAAGGAAGAA
    GCCCCAAAGC CACTTCACAA AGTAGTGGTA TGTGTTAGTA AAAAACTCAG TAAGAAGCAG
    AGTGAACTAA ATGGGATCGC AGCCTCTCTA GGAGCAGATT ACAGGTGGAG TTTTGATGAA
    ACAGTGACTC ATTTCATCTA TCAAGGGCGG CCAAATGACA CTAATCGGGA GTATAAATCT
    GTAAAAGAAA GAGGAGTACA CATTGTTTCC GAGCACTGGC TTTTAGATTG TGCCCAAGAG
    TGTAAACATC TTCCTGAATC TCTTTATCCA CATACTTATA ATCCCAAAAT GAGCTTGGAT
    ATCAGCGCAG TGCAAGATGG CCGGCTCTGT AATAGTCGAC TACTCTCAGC TGTGTCTTCA
    ACAAAGGATG ATGAGCCAGA TCCTTTGATT TTAGAAGAAA ATGATGTAGA CAATATGGCC
    ACCAATAATA AAGAGTCAGC ACCATCAAAT GGAAGTGGAA AGAATGACTC TAAAGGAGTT
    CTGACACAGA CCTTAGAGAT GAGAGAGAAC TTTCAGAAGC AGTTACAGGA GATAATGTCT
    GCAACATCAA TAGTGAAACC CCAAGGGCAG AGGACTTCCC TTTCAAGAAG TGGTTGTAAC
    AGCGCATCTT CAACCCCTGA CAGCACTCGC TCTGCTCGCA GTGGACGAAG TAGAGTCCTA
    GAGGCACTGA GGCAGTCTCG TCAGACAGTA CCTGATGTCA ACACAGAGCC TTCCCAAAAT
    GAACAGATCA TTTGGGATGA CCCTACAGCA AGGGAGGAGA GAGCAAGGCT TGCCAGCAAT
    TTGCAGTGGC CTAGTTGTCC CACACAATAC TCTGAGCTTC AGGTTGACAT TCAAAACTTG
    GAGGATTCTC CTTTTCAAAA GCCTTTACAT GATTCAGAAA TTGCTAAACA GGCTGTCTGT
    GATCCTGGAA ACATACGTGT GACTGAAGCT CCCAAACACC CAATCTCTGA AGAACTGGAA
    ACTCCCATAA AAGACAGCCA CCTGATCCCT ACGCCTCAAG CCCCCAGTAT TGCCTTTCCA
    CTCGCCAACC CCCCTGTGGC TCCGCACCCT AGAGAAAAGA TTATAACGAT AGAGGAGACT
    CATGAAGAAT TAAAAAAACA GTACATATTT CAGTTATCAT CTCTGAATCC TCAAGAACGT
    ATTGACTATT GTCATCTGAT TGAGAAACTA GGTGGATTGG TGATAGAAAA GCAGTGCTTT
    GATCCCACCT GTACACACAT TGTTGTGGGA CATCCACTTC GAAACGAGAA GTATTTAGCC
    TCAGTGGCAG CTGGGAAGTG GGTGCTTCAT CGCTCCTACC TTGAAGCCTG CAGGACTGCT
    GGACACTTCG TGCAGGAAGA AGACTATGAA TGGGGAAGTA GTTCCATACT TGATGTTCTG
    ACTGGAATCA ATGTACAGCA ACGAAGACTA GCACTTGCAG CAATGAGATG GAGAAAAAAA
    ATCCAGCAAA GACAAGAATC TGGCATTGTT GAGGGAGCAT TTAGTGGGTG GAAGGTTATT
    TTACATGTGG ATCAGTCTCG AGAAGCAGGC TTCAAACGCC TTCTTCAGTC AGGAGGAGCA
    AAGGTGCTAC CTGGTCATTC TGTACCTTTA TTTAAAGAGG CCACACATCT TTTTTCTGAC
    TTGAATAAAC TGAAACCAGA TGACTCAGGA GTTAATATAG CAGAAGCTGC TGCCCAGAAC
    GTGTACTGCT TGAGAACAGA ATACATTGCT GATTATCTCA TGCAGGAATC ACCTCCTCAT
    GTAGAAAATT ACTGTCTACC AGAAGCTATT TCATTTATTC AGAATAATAA GGAACTTGGG
    ACTGGATTAT CACAAAAGAG GAAAGCTCCT ACAGAAAAAA ATAAAATCAA ACGACCTAGA
    GTACACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu16438
    Clone ID Related Accession (Same CDS sequence) XM_017005636.1 , XM_017005636.2 , NM_007027.3 , NM_007027.4
    Accession Version XM_017005636.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4569bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product DNA topoisomerase 2-binding protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017005636.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGTCCAGAA ATGACAAAGA ACCGTTTTTT GTGAAGTTTT TAAAGTCTTC AGACAATTCC 
    AAATGTTTTT TTAAAGCTCT CGAGTCCATA AAAGAATTCC AATCAGAAGA ATATCTTCAG
    ATTATTACAG AAGAAGAGGC ATTGAAGATA AAGGAGAATG ATAGATCACT TTATATCTGT
    GACCCTTTTA GTGGCGTTGT CTTTGATCAC CTCAAAAAGC TTGGCTGCAG AATTGTTGGT
    CCTCAAGTAG TCATATTTTG TATGCACCAC CAGCGATGTG TCCCAAGAGC CGAACATCCA
    GTTTATAATA TGGTTATGTC TGATGTAACC ATATCTTGTA CAAGTCTGGA AAAAGAAAAA
    AGGGAAGAAG TTCATAAATA TGTACAAATG ATGGGCGGAC GAGTATACAG AGACCTTAAT
    GTATCAGTAA CTCACCTTAT TGCAGGAGAA GTTGGTAGCA AAAAATATTT AGTTGCTGCA
    AACCTGAAGA AACCTATTTT GCTTCCCTCT TGGATAAAAA CACTTTGGGA GAAGTCACAA
    GAGAAAAAAA TAACTAGATA TACTGATATA AACATGGAAG ATTTCAAGTG TCCTATTTTT
    CTTGGTTGCA TAATCTGTGT GACTGGCTTA TGTGGCTTAG ACAGGAAAGA AGTTCAGCAA
    CTCACAGTTA AGCATGGAGG TCAATACATG GGACAATTGA AAATGAATGA ATGTACACAC
    CTCATTGTGC AAGAACCAAA AGGTCAGAAG TATGAGTGTG CCAAGAGATG GAATGTACAC
    TGTGTGACCA CACAGTGGTT TTTTGACAGT ATTGAGAAAG GTTTTTGTCA GGATGAATCC
    ATATACAAGA CAGAACCTAG ACCAGAAGCA AAGACTATGC CCAATTCTTC AACTCCTACC
    AGCCAGATCA ACACAATTGA TAGTCGTACT CTTTCAGATG TCAGCAATAT TTCCAACATA
    AATGCAAGTT GCGTAAGTGA ATCAATATGT AATTCACTTA ACAGCAAACT GGAGCCTACA
    CTTGAAAATC TAGAAAATCT GGATGTCAGT GCATTTCAAG CACCTGAAGA TTTATTAGAT
    GGTTGTCGGA TATATCTTTG CGGTTTTAGT GGCAGAAAGC TAGATAAACT GAGAAGACTT
    ATTAACAGTG GAGGTGGAGT TCGTTTTAAC CAGCTAAATG AAGATGTAAC TCATGTTATT
    GTGGGAGATT ATGATGATGA ATTGAAGCAG TTTTGGAATA AATCAGCCCA CAGGCCTCAT
    GTAGTGGGAG CAAAGTGGTT GCTAGAGTGT TTCAGTAAAG GTTATATGCT TTCTGAAGAA
    CCATATATCC ATGCTAATTA CCAGCCAGTG GAAATTCCAG TTTCACATAA GCCTGAAAGT
    AAAGCAGCTC TTTTAAAAAA GAAGAACAGC AGCTTCTCTA AGAAAGACTT TGCTCCTAGT
    GAAAAGCATG AGCAAGCTGA TGAAGATCTG CTCTCTCAAT ATGAAAATGG TAGCTCCACA
    GTAGTTGAGG CTAAGACGTC TGAAGCCAGG CCCTTTAATG ATTCTACTCA TGCTGAGCCC
    TTGAATGATT CTACTCACAT TTCTTTGCAA GAAGAAAACC AGTCTTCTGT CAGTCATTGT
    GTCCCTGATG TTTCTACAAT TACTGAAGAA GGCTTATTTA GCCAAAAGAG TTTCCTTGTT
    TTGGGTTTTA GTAATGAAAA TGAATCTAAC ATCGCAAACA TCATAAAAGA AAATGCTGGG
    AAAATCATGT CCCTTCTGAG CAGAACTGTT GCGGATTATG CTGTGGTTCC TCTGCTGGGG
    TGTGAAGTGG AAGCCACTGT GGGAGAAGTT GTTACAAATA CATGGCTGGT TACTTGCATA
    GACTATCAGA CTTTGTTTGA TCCAAAGTCG AATCCTCTCT TCACACCAGT TCCAGTAATG
    ACAGGAATGA CTCCTTTAGA GGATTGTGTT ATTTCATTTA GCCAGTGTGC TGGAGCAGAA
    AAAGAGTCTT TAACATTCCT AGCAAACCTC CTTGGAGCAA GTGTTCAAGA ATACTTTGTT
    CGCAAATCCA ATGCAAAGAA AGGCATGTTT GCCAGTACTC ATCTTATACT GAAAGAACGT
    GGTGGCTCTA AATATGAAGC TGCAAAGAAG TGGAATTTAC CTGCCGTTAC TATAGCTTGG
    CTGTTGGAGA CTGCTAGAAC GGGAAAGAGA GCAGACGAAA GCCATTTTCT GATTGAAAAT
    TCAACTAAAG AAGAACGAAG TTTGGAAACA GAAATAACAA ATGGAATCAA TCTAAATTCA
    GATACTGCAG AGCATCCTGG CACACGCCTG CAAACTCACA GAAAAACCGT CGTTACACCT
    TTAGATATGA ACCGCTTTCA GAGTAAAGCT TTCCGTGCTG TGGTCTCACA ACATGCCAGA
    CAGGTCGCAG CCTCCCCAGC AGTAGGACAA CCACTTCAGA AGGAGCCCTC GTTACACCTG
    GATACACCAT CAAAATTCCT GTCCAAGGAC AAACTCTTCA AGCCTTCCTT TGATGTGAAG
    GATGCACTTG CAGCCTTGGA AACTCCAGGA CGTCCCAGCC AACAGAAAAG GAAACCGAGT
    ACGCCACTCT CAGAAGTTAT TGTCAAAAAC TTGCAACTTG CTTTGGCAAA TAGCTCTCGA
    AATGCTGTCG CTCTTTCTGC CAGCCCTCAA CTGAAAGAGG CCCAGTCAGA GAAGGAAGAA
    GCCCCAAAGC CACTTCACAA AGTAGTGGTA TGTGTTAGTA AAAAACTCAG TAAGAAGCAG
    AGTGAACTAA ATGGGATCGC AGCCTCTCTA GGAGCAGATT ACAGGTGGAG TTTTGATGAA
    ACAGTGACTC ATTTCATCTA TCAAGGGCGG CCAAATGACA CTAATCGGGA GTATAAATCT
    GTAAAAGAAA GAGGAGTACA CATTGTTTCC GAGCACTGGC TTTTAGATTG TGCCCAAGAG
    TGTAAACATC TTCCTGAATC TCTTTATCCA CATACTTATA ATCCCAAAAT GAGCTTGGAT
    ATCAGCGCAG TGCAAGATGG CCGGCTCTGT AATAGTCGAC TACTCTCAGC TGTGTCTTCA
    ACAAAGGATG ATGAGCCAGA TCCTTTGATT TTAGAAGAAA ATGATGTAGA CAATATGGCC
    ACCAATAATA AAGAGTCAGC ACCATCAAAT GGAAGTGGAA AGAATGACTC TAAAGGAGTT
    CTGACACAGA CCTTAGAGAT GAGAGAGAAC TTTCAGAAGC AGTTACAGGA GATAATGTCT
    GCAACATCAA TAGTGAAACC CCAAGGGCAG AGGACTTCCC TTTCAAGAAG TGGTTGTAAC
    AGCGCATCTT CAACCCCTGA CAGCACTCGC TCTGCTCGCA GTGGACGAAG TAGAGTCCTA
    GAGGCACTGA GGCAGTCTCG TCAGACAGTA CCTGATGTCA ACACAGAGCC TTCCCAAAAT
    GAACAGATCA TTTGGGATGA CCCTACAGCA AGGGAGGAGA GAGCAAGGCT TGCCAGCAAT
    TTGCAGTGGC CTAGTTGTCC CACACAATAC TCTGAGCTTC AGGTTGACAT TCAAAACTTG
    GAGGATTCTC CTTTTCAAAA GCCTTTACAT GATTCAGAAA TTGCTAAACA GGCTGTCTGT
    GATCCTGGAA ACATACGTGT GACTGAAGCT CCCAAACACC CAATCTCTGA AGAACTGGAA
    ACTCCCATAA AAGACAGCCA CCTGATCCCT ACGCCTCAAG CCCCCAGTAT TGCCTTTCCA
    CTCGCCAACC CCCCTGTGGC TCCGCACCCT AGAGAAAAGA TTATAACGAT AGAGGAGACT
    CATGAAGAAT TAAAAAAACA GTACATATTT CAGTTATCAT CTCTGAATCC TCAAGAACGT
    ATTGACTATT GTCATCTGAT TGAGAAACTA GGTGGATTGG TGATAGAAAA GCAGTGCTTT
    GATCCCACCT GTACACACAT TGTTGTGGGA CATCCACTTC GAAACGAGAA GTATTTAGCC
    TCAGTGGCAG CTGGGAAGTG GGTGCTTCAT CGCTCCTACC TTGAAGCCTG CAGGACTGCT
    GGACACTTCG TGCAGGAAGA AGACTATGAA TGGGGAAGTA GTTCCATACT TGATGTTCTG
    ACTGGAATCA ATGTACAGCA ACGAAGACTA GCACTTGCAG CAATGAGATG GAGAAAAAAA
    ATCCAGCAAA GACAAGAATC TGGCATTGTT GAGGGAGCAT TTAGTGGGTG GAAGGTTATT
    TTACATGTGG ATCAGTCTCG AGAAGCAGGC TTCAAACGCC TTCTTCAGTC AGGAGGAGCA
    AAGGTGCTAC CTGGTCATTC TGTACCTTTA TTTAAAGAGG CCACACATCT TTTTTCTGAC
    TTGAATAAAC TGAAACCAGA TGACTCAGGA GTTAATATAG CAGAAGCTGC TGCCCAGAAC
    GTGTACTGCT TGAGAACAGA ATACATTGCT GATTATCTCA TGCAGGAATC ACCTCCTCAT
    GTAGAAAATT ACTGTCTACC AGAAGCTATT TCATTTATTC AGAATAATAA GGAACTTGGG
    ACTGGATTAT CACAAAAGAG GAAAGCTCCT ACAGAAAAAA ATAAAATCAA ACGACCTAGA
    GTACACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016861125.1
    CDS203..4771
    Translation

    Target ORF information:

    RefSeq Version XM_017005636.2
    Organism Homo sapiens(human)
    Definition Homo sapiens DNA topoisomerase II binding protein 1 (TOPBP1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017005636.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGTCCAGAA ATGACAAAGA ACCGTTTTTT GTGAAGTTTT TAAAGTCTTC AGACAATTCC 
    AAATGTTTTT TTAAAGCTCT CGAGTCCATA AAAGAATTCC AATCAGAAGA ATATCTTCAG
    ATTATTACAG AAGAAGAGGC ATTGAAGATA AAGGAGAATG ATAGATCACT TTATATCTGT
    GACCCTTTTA GTGGCGTTGT CTTTGATCAC CTCAAAAAGC TTGGCTGCAG AATTGTTGGT
    CCTCAAGTAG TCATATTTTG TATGCACCAC CAGCGATGTG TCCCAAGAGC CGAACATCCA
    GTTTATAATA TGGTTATGTC TGATGTAACC ATATCTTGTA CAAGTCTGGA AAAAGAAAAA
    AGGGAAGAAG TTCATAAATA TGTACAAATG ATGGGCGGAC GAGTATACAG AGACCTTAAT
    GTATCAGTAA CTCACCTTAT TGCAGGAGAA GTTGGTAGCA AAAAATATTT AGTTGCTGCA
    AACCTGAAGA AACCTATTTT GCTTCCCTCT TGGATAAAAA CACTTTGGGA GAAGTCACAA
    GAGAAAAAAA TAACTAGATA TACTGATATA AACATGGAAG ATTTCAAGTG TCCTATTTTT
    CTTGGTTGCA TAATCTGTGT GACTGGCTTA TGTGGCTTAG ACAGGAAAGA AGTTCAGCAA
    CTCACAGTTA AGCATGGAGG TCAATACATG GGACAATTGA AAATGAATGA ATGTACACAC
    CTCATTGTGC AAGAACCAAA AGGTCAGAAG TATGAGTGTG CCAAGAGATG GAATGTACAC
    TGTGTGACCA CACAGTGGTT TTTTGACAGT ATTGAGAAAG GTTTTTGTCA GGATGAATCC
    ATATACAAGA CAGAACCTAG ACCAGAAGCA AAGACTATGC CCAATTCTTC AACTCCTACC
    AGCCAGATCA ACACAATTGA TAGTCGTACT CTTTCAGATG TCAGCAATAT TTCCAACATA
    AATGCAAGTT GCGTAAGTGA ATCAATATGT AATTCACTTA ACAGCAAACT GGAGCCTACA
    CTTGAAAATC TAGAAAATCT GGATGTCAGT GCATTTCAAG CACCTGAAGA TTTATTAGAT
    GGTTGTCGGA TATATCTTTG CGGTTTTAGT GGCAGAAAGC TAGATAAACT GAGAAGACTT
    ATTAACAGTG GAGGTGGAGT TCGTTTTAAC CAGCTAAATG AAGATGTAAC TCATGTTATT
    GTGGGAGATT ATGATGATGA ATTGAAGCAG TTTTGGAATA AATCAGCCCA CAGGCCTCAT
    GTAGTGGGAG CAAAGTGGTT GCTAGAGTGT TTCAGTAAAG GTTATATGCT TTCTGAAGAA
    CCATATATCC ATGCTAATTA CCAGCCAGTG GAAATTCCAG TTTCACATAA GCCTGAAAGT
    AAAGCAGCTC TTTTAAAAAA GAAGAACAGC AGCTTCTCTA AGAAAGACTT TGCTCCTAGT
    GAAAAGCATG AGCAAGCTGA TGAAGATCTG CTCTCTCAAT ATGAAAATGG TAGCTCCACA
    GTAGTTGAGG CTAAGACGTC TGAAGCCAGG CCCTTTAATG ATTCTACTCA TGCTGAGCCC
    TTGAATGATT CTACTCACAT TTCTTTGCAA GAAGAAAACC AGTCTTCTGT CAGTCATTGT
    GTCCCTGATG TTTCTACAAT TACTGAAGAA GGCTTATTTA GCCAAAAGAG TTTCCTTGTT
    TTGGGTTTTA GTAATGAAAA TGAATCTAAC ATCGCAAACA TCATAAAAGA AAATGCTGGG
    AAAATCATGT CCCTTCTGAG CAGAACTGTT GCGGATTATG CTGTGGTTCC TCTGCTGGGG
    TGTGAAGTGG AAGCCACTGT GGGAGAAGTT GTTACAAATA CATGGCTGGT TACTTGCATA
    GACTATCAGA CTTTGTTTGA TCCAAAGTCG AATCCTCTCT TCACACCAGT TCCAGTAATG
    ACAGGAATGA CTCCTTTAGA GGATTGTGTT ATTTCATTTA GCCAGTGTGC TGGAGCAGAA
    AAAGAGTCTT TAACATTCCT AGCAAACCTC CTTGGAGCAA GTGTTCAAGA ATACTTTGTT
    CGCAAATCCA ATGCAAAGAA AGGCATGTTT GCCAGTACTC ATCTTATACT GAAAGAACGT
    GGTGGCTCTA AATATGAAGC TGCAAAGAAG TGGAATTTAC CTGCCGTTAC TATAGCTTGG
    CTGTTGGAGA CTGCTAGAAC GGGAAAGAGA GCAGACGAAA GCCATTTTCT GATTGAAAAT
    TCAACTAAAG AAGAACGAAG TTTGGAAACA GAAATAACAA ATGGAATCAA TCTAAATTCA
    GATACTGCAG AGCATCCTGG CACACGCCTG CAAACTCACA GAAAAACCGT CGTTACACCT
    TTAGATATGA ACCGCTTTCA GAGTAAAGCT TTCCGTGCTG TGGTCTCACA ACATGCCAGA
    CAGGTCGCAG CCTCCCCAGC AGTAGGACAA CCACTTCAGA AGGAGCCCTC GTTACACCTG
    GATACACCAT CAAAATTCCT GTCCAAGGAC AAACTCTTCA AGCCTTCCTT TGATGTGAAG
    GATGCACTTG CAGCCTTGGA AACTCCAGGA CGTCCCAGCC AACAGAAAAG GAAACCGAGT
    ACGCCACTCT CAGAAGTTAT TGTCAAAAAC TTGCAACTTG CTTTGGCAAA TAGCTCTCGA
    AATGCTGTCG CTCTTTCTGC CAGCCCTCAA CTGAAAGAGG CCCAGTCAGA GAAGGAAGAA
    GCCCCAAAGC CACTTCACAA AGTAGTGGTA TGTGTTAGTA AAAAACTCAG TAAGAAGCAG
    AGTGAACTAA ATGGGATCGC AGCCTCTCTA GGAGCAGATT ACAGGTGGAG TTTTGATGAA
    ACAGTGACTC ATTTCATCTA TCAAGGGCGG CCAAATGACA CTAATCGGGA GTATAAATCT
    GTAAAAGAAA GAGGAGTACA CATTGTTTCC GAGCACTGGC TTTTAGATTG TGCCCAAGAG
    TGTAAACATC TTCCTGAATC TCTTTATCCA CATACTTATA ATCCCAAAAT GAGCTTGGAT
    ATCAGCGCAG TGCAAGATGG CCGGCTCTGT AATAGTCGAC TACTCTCAGC TGTGTCTTCA
    ACAAAGGATG ATGAGCCAGA TCCTTTGATT TTAGAAGAAA ATGATGTAGA CAATATGGCC
    ACCAATAATA AAGAGTCAGC ACCATCAAAT GGAAGTGGAA AGAATGACTC TAAAGGAGTT
    CTGACACAGA CCTTAGAGAT GAGAGAGAAC TTTCAGAAGC AGTTACAGGA GATAATGTCT
    GCAACATCAA TAGTGAAACC CCAAGGGCAG AGGACTTCCC TTTCAAGAAG TGGTTGTAAC
    AGCGCATCTT CAACCCCTGA CAGCACTCGC TCTGCTCGCA GTGGACGAAG TAGAGTCCTA
    GAGGCACTGA GGCAGTCTCG TCAGACAGTA CCTGATGTCA ACACAGAGCC TTCCCAAAAT
    GAACAGATCA TTTGGGATGA CCCTACAGCA AGGGAGGAGA GAGCAAGGCT TGCCAGCAAT
    TTGCAGTGGC CTAGTTGTCC CACACAATAC TCTGAGCTTC AGGTTGACAT TCAAAACTTG
    GAGGATTCTC CTTTTCAAAA GCCTTTACAT GATTCAGAAA TTGCTAAACA GGCTGTCTGT
    GATCCTGGAA ACATACGTGT GACTGAAGCT CCCAAACACC CAATCTCTGA AGAACTGGAA
    ACTCCCATAA AAGACAGCCA CCTGATCCCT ACGCCTCAAG CCCCCAGTAT TGCCTTTCCA
    CTCGCCAACC CCCCTGTGGC TCCGCACCCT AGAGAAAAGA TTATAACGAT AGAGGAGACT
    CATGAAGAAT TAAAAAAACA GTACATATTT CAGTTATCAT CTCTGAATCC TCAAGAACGT
    ATTGACTATT GTCATCTGAT TGAGAAACTA GGTGGATTGG TGATAGAAAA GCAGTGCTTT
    GATCCCACCT GTACACACAT TGTTGTGGGA CATCCACTTC GAAACGAGAA GTATTTAGCC
    TCAGTGGCAG CTGGGAAGTG GGTGCTTCAT CGCTCCTACC TTGAAGCCTG CAGGACTGCT
    GGACACTTCG TGCAGGAAGA AGACTATGAA TGGGGAAGTA GTTCCATACT TGATGTTCTG
    ACTGGAATCA ATGTACAGCA ACGAAGACTA GCACTTGCAG CAATGAGATG GAGAAAAAAA
    ATCCAGCAAA GACAAGAATC TGGCATTGTT GAGGGAGCAT TTAGTGGGTG GAAGGTTATT
    TTACATGTGG ATCAGTCTCG AGAAGCAGGC TTCAAACGCC TTCTTCAGTC AGGAGGAGCA
    AAGGTGCTAC CTGGTCATTC TGTACCTTTA TTTAAAGAGG CCACACATCT TTTTTCTGAC
    TTGAATAAAC TGAAACCAGA TGACTCAGGA GTTAATATAG CAGAAGCTGC TGCCCAGAAC
    GTGTACTGCT TGAGAACAGA ATACATTGCT GATTATCTCA TGCAGGAATC ACCTCCTCAT
    GTAGAAAATT ACTGTCTACC AGAAGCTATT TCATTTATTC AGAATAATAA GGAACTTGGG
    ACTGGATTAT CACAAAAGAG GAAAGCTCCT ACAGAAAAAA ATAAAATCAA ACGACCTAGA
    GTACACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu93765
    Clone ID Related Accession (Same CDS sequence) XM_017005637.1 , XM_017005637.2
    Accession Version XM_017005637.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3264bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product DNA topoisomerase 2-binding protein 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGCTTTCTG AAGAACCATA TATCCATGCT AATTACCAGC CAGTGGAAAT TCCAGTTTCA 
    CATAAGCCTG AAAGTAAAGC AGCTCTTTTA AAAAAGAAGA ACAGCAGCTT CTCTAAGAAA
    GACTTTGCTC CTAGTGAAAA GCATGAGCAA GCTGATGAAG ATCTGCTCTC TCAATATGAA
    AATGGTAGCT CCACAGTAGT TGAGGCTAAG ACGTCTGAAG CCAGGCCCTT TAATGATTCT
    ACTCATGCTG AGCCCTTGAA TGATTCTACT CACATTTCTT TGCAAGAAGA AAACCAGTCT
    TCTGTCAGTC ATTGTGTCCC TGATGTTTCT ACAATTACTG AAGAAGGCTT ATTTAGCCAA
    AAGAGTTTCC TTGTTTTGGG TTTTAGTAAT GAAAATGAAT CTAACATCGC AAACATCATA
    AAAGAAAATG CTGGGAAAAT CATGTCCCTT CTGAGCAGAA CTGTTGCGGA TTATGCTGTG
    GTTCCTCTGC TGGGGTGTGA AGTGGAAGCC ACTGTGGGAG AAGTTGTTAC AAATACATGG
    CTGGTTACTT GCATAGACTA TCAGACTTTG TTTGATCCAA AGTCGAATCC TCTCTTCACA
    CCAGTTCCAG TAATGACAGG AATGACTCCT TTAGAGGATT GTGTTATTTC ATTTAGCCAG
    TGTGCTGGAG CAGAAAAAGA GTCTTTAACA TTCCTAGCAA ACCTCCTTGG AGCAAGTGTT
    CAAGAATACT TTGTTCGCAA ATCCAATGCA AAGAAAGGCA TGTTTGCCAG TACTCATCTT
    ATACTGAAAG AACGTGGTGG CTCTAAATAT GAAGCTGCAA AGAAGTGGAA TTTACCTGCC
    GTTACTATAG CTTGGCTGTT GGAGACTGCT AGAACGGGAA AGAGAGCAGA CGAAAGCCAT
    TTTCTGATTG AAAATTCAAC TAAAGAAGAA CGAAGTTTGG AAACAGAAAT AACAAATGGA
    ATCAATCTAA ATTCAGATAC TGCAGAGCAT CCTGGCACAC GCCTGCAAAC TCACAGAAAA
    ACCGTCGTTA CACCTTTAGA TATGAACCGC TTTCAGAGTA AAGCTTTCCG TGCTGTGGTC
    TCACAACATG CCAGACAGGT CGCAGCCTCC CCAGCAGTAG GACAACCACT TCAGAAGGAG
    CCCTCGTTAC ACCTGGATAC ACCATCAAAA TTCCTGTCCA AGGACAAACT CTTCAAGCCT
    TCCTTTGATG TGAAGGATGC ACTTGCAGCC TTGGAAACTC CAGGACGTCC CAGCCAACAG
    AAAAGGAAAC CGAGTACGCC ACTCTCAGAA GTTATTGTCA AAAACTTGCA ACTTGCTTTG
    GCAAATAGCT CTCGAAATGC TGTCGCTCTT TCTGCCAGCC CTCAACTGAA AGAGGCCCAG
    TCAGAGAAGG AAGAAGCCCC AAAGCCACTT CACAAAGTAG TGGTATGTGT TAGTAAAAAA
    CTCAGTAAGA AGCAGAGTGA ACTAAATGGG ATCGCAGCCT CTCTAGGAGC AGATTACAGG
    TGGAGTTTTG ATGAAACAGT GACTCATTTC ATCTATCAAG GGCGGCCAAA TGACACTAAT
    CGGGAGTATA AATCTGTAAA AGAAAGAGGA GTACACATTG TTTCCGAGCA CTGGCTTTTA
    GATTGTGCCC AAGAGTGTAA ACATCTTCCT GAATCTCTTT ATCCACATAC TTATAATCCC
    AAAATGAGCT TGGATATCAG CGCAGTGCAA GATGGCCGGC TCTGTAATAG TCGACTACTC
    TCAGCTGTGT CTTCAACAAA GGATGATGAG CCAGATCCTT TGATTTTAGA AGAAAATGAT
    GTAGACAATA TGGCCACCAA TAATAAAGAG TCAGCACCAT CAAATGGAAG TGGAAAGAAT
    GACTCTAAAG GAGTTCTGAC ACAGACCTTA GAGATGAGAG AGAACTTTCA GAAGCAGTTA
    CAGGAGATAA TGTCTGCAAC ATCAATAGTG AAACCCCAAG GGCAGAGGAC TTCCCTTTCA
    AGAAGTGGTT GTAACAGCGC ATCTTCAACC CCTGACAGCA CTCGCTCTGC TCGCAGTGGA
    CGAAGTAGAG TCCTAGAGGC ACTGAGGCAG TCTCGTCAGA CAGTACCTGA TGTCAACACA
    GAGCCTTCCC AAAATGAACA GATCATTTGG GATGACCCTA CAGCAAGGGA GGAGAGAGCA
    AGGCTTGCCA GCAATTTGCA GTGGCCTAGT TGTCCCACAC AATACTCTGA GCTTCAGGTT
    GACATTCAAA ACTTGGAGGA TTCTCCTTTT CAAAAGCCTT TACATGATTC AGAAATTGCT
    AAACAGGCTG TCTGTGATCC TGGAAACATA CGTGTGACTG AAGCTCCCAA ACACCCAATC
    TCTGAAGAAC TGGAAACTCC CATAAAAGAC AGCCACCTGA TCCCTACGCC TCAAGCCCCC
    AGTATTGCCT TTCCACTCGC CAACCCCCCT GTGGCTCCGC ACCCTAGAGA AAAGATTATA
    ACGATAGAGG AGACTCATGA AGAATTAAAA AAACAGTACA TATTTCAGTT ATCATCTCTG
    AATCCTCAAG AACGTATTGA CTATTGTCAT CTGATTGAGA AACTAGGTGG ATTGGTGATA
    GAAAAGCAGT GCTTTGATCC CACCTGTACA CACATTGTTG TGGGACATCC ACTTCGAAAC
    GAGAAGTATT TAGCCTCAGT GGCAGCTGGG AAGTGGGTGC TTCATCGCTC CTACCTTGAA
    GCCTGCAGGA CTGCTGGACA CTTCGTGCAG GAAGAAGACT ATGAATGGGG AAGTAGTTCC
    ATACTTGATG TTCTGACTGG AATCAATGTA CAGCAACGAA GACTAGCACT TGCAGCAATG
    AGATGGAGAA AAAAAATCCA GCAAAGACAA GAATCTGGCA TTGTTGAGGG AGCATTTAGT
    GGGTGGAAGG TTATTTTACA TGTGGATCAG TCTCGAGAAG CAGGCTTCAA ACGCCTTCTT
    CAGTCAGGAG GAGCAAAGGT GCTACCTGGT CATTCTGTAC CTTTATTTAA AGAGGCCACA
    CATCTTTTTT CTGACTTGAA TAAACTGAAA CCAGATGACT CAGGAGTTAA TATAGCAGAA
    GCTGCTGCCC AGAACGTGTA CTGCTTGAGA ACAGAATACA TTGCTGATTA TCTCATGCAG
    GAATCACCTC CTCATGTAGA AAATTACTGT CTACCAGAAG CTATTTCATT TATTCAGAAT
    AATAAGGAAC TTGGGACTGG ATTATCACAA AAGAGGAAAG CTCCTACAGA AAAAAATAAA
    ATCAAACGAC CTAGAGTACA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016861126.1
    CDS962..4225
    Translation

    Target ORF information:

    RefSeq Version XM_017005637.1
    Organism Homo sapiens(human)
    Definition Homo sapiens topoisomerase (DNA) II binding protein 1 (TOPBP1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017005637.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGCTTTCTG AAGAACCATA TATCCATGCT AATTACCAGC CAGTGGAAAT TCCAGTTTCA 
    CATAAGCCTG AAAGTAAAGC AGCTCTTTTA AAAAAGAAGA ACAGCAGCTT CTCTAAGAAA
    GACTTTGCTC CTAGTGAAAA GCATGAGCAA GCTGATGAAG ATCTGCTCTC TCAATATGAA
    AATGGTAGCT CCACAGTAGT TGAGGCTAAG ACGTCTGAAG CCAGGCCCTT TAATGATTCT
    ACTCATGCTG AGCCCTTGAA TGATTCTACT CACATTTCTT TGCAAGAAGA AAACCAGTCT
    TCTGTCAGTC ATTGTGTCCC TGATGTTTCT ACAATTACTG AAGAAGGCTT ATTTAGCCAA
    AAGAGTTTCC TTGTTTTGGG TTTTAGTAAT GAAAATGAAT CTAACATCGC AAACATCATA
    AAAGAAAATG CTGGGAAAAT CATGTCCCTT CTGAGCAGAA CTGTTGCGGA TTATGCTGTG
    GTTCCTCTGC TGGGGTGTGA AGTGGAAGCC ACTGTGGGAG AAGTTGTTAC AAATACATGG
    CTGGTTACTT GCATAGACTA TCAGACTTTG TTTGATCCAA AGTCGAATCC TCTCTTCACA
    CCAGTTCCAG TAATGACAGG AATGACTCCT TTAGAGGATT GTGTTATTTC ATTTAGCCAG
    TGTGCTGGAG CAGAAAAAGA GTCTTTAACA TTCCTAGCAA ACCTCCTTGG AGCAAGTGTT
    CAAGAATACT TTGTTCGCAA ATCCAATGCA AAGAAAGGCA TGTTTGCCAG TACTCATCTT
    ATACTGAAAG AACGTGGTGG CTCTAAATAT GAAGCTGCAA AGAAGTGGAA TTTACCTGCC
    GTTACTATAG CTTGGCTGTT GGAGACTGCT AGAACGGGAA AGAGAGCAGA CGAAAGCCAT
    TTTCTGATTG AAAATTCAAC TAAAGAAGAA CGAAGTTTGG AAACAGAAAT AACAAATGGA
    ATCAATCTAA ATTCAGATAC TGCAGAGCAT CCTGGCACAC GCCTGCAAAC TCACAGAAAA
    ACCGTCGTTA CACCTTTAGA TATGAACCGC TTTCAGAGTA AAGCTTTCCG TGCTGTGGTC
    TCACAACATG CCAGACAGGT CGCAGCCTCC CCAGCAGTAG GACAACCACT TCAGAAGGAG
    CCCTCGTTAC ACCTGGATAC ACCATCAAAA TTCCTGTCCA AGGACAAACT CTTCAAGCCT
    TCCTTTGATG TGAAGGATGC ACTTGCAGCC TTGGAAACTC CAGGACGTCC CAGCCAACAG
    AAAAGGAAAC CGAGTACGCC ACTCTCAGAA GTTATTGTCA AAAACTTGCA ACTTGCTTTG
    GCAAATAGCT CTCGAAATGC TGTCGCTCTT TCTGCCAGCC CTCAACTGAA AGAGGCCCAG
    TCAGAGAAGG AAGAAGCCCC AAAGCCACTT CACAAAGTAG TGGTATGTGT TAGTAAAAAA
    CTCAGTAAGA AGCAGAGTGA ACTAAATGGG ATCGCAGCCT CTCTAGGAGC AGATTACAGG
    TGGAGTTTTG ATGAAACAGT GACTCATTTC ATCTATCAAG GGCGGCCAAA TGACACTAAT
    CGGGAGTATA AATCTGTAAA AGAAAGAGGA GTACACATTG TTTCCGAGCA CTGGCTTTTA
    GATTGTGCCC AAGAGTGTAA ACATCTTCCT GAATCTCTTT ATCCACATAC TTATAATCCC
    AAAATGAGCT TGGATATCAG CGCAGTGCAA GATGGCCGGC TCTGTAATAG TCGACTACTC
    TCAGCTGTGT CTTCAACAAA GGATGATGAG CCAGATCCTT TGATTTTAGA AGAAAATGAT
    GTAGACAATA TGGCCACCAA TAATAAAGAG TCAGCACCAT CAAATGGAAG TGGAAAGAAT
    GACTCTAAAG GAGTTCTGAC ACAGACCTTA GAGATGAGAG AGAACTTTCA GAAGCAGTTA
    CAGGAGATAA TGTCTGCAAC ATCAATAGTG AAACCCCAAG GGCAGAGGAC TTCCCTTTCA
    AGAAGTGGTT GTAACAGCGC ATCTTCAACC CCTGACAGCA CTCGCTCTGC TCGCAGTGGA
    CGAAGTAGAG TCCTAGAGGC ACTGAGGCAG TCTCGTCAGA CAGTACCTGA TGTCAACACA
    GAGCCTTCCC AAAATGAACA GATCATTTGG GATGACCCTA CAGCAAGGGA GGAGAGAGCA
    AGGCTTGCCA GCAATTTGCA GTGGCCTAGT TGTCCCACAC AATACTCTGA GCTTCAGGTT
    GACATTCAAA ACTTGGAGGA TTCTCCTTTT CAAAAGCCTT TACATGATTC AGAAATTGCT
    AAACAGGCTG TCTGTGATCC TGGAAACATA CGTGTGACTG AAGCTCCCAA ACACCCAATC
    TCTGAAGAAC TGGAAACTCC CATAAAAGAC AGCCACCTGA TCCCTACGCC TCAAGCCCCC
    AGTATTGCCT TTCCACTCGC CAACCCCCCT GTGGCTCCGC ACCCTAGAGA AAAGATTATA
    ACGATAGAGG AGACTCATGA AGAATTAAAA AAACAGTACA TATTTCAGTT ATCATCTCTG
    AATCCTCAAG AACGTATTGA CTATTGTCAT CTGATTGAGA AACTAGGTGG ATTGGTGATA
    GAAAAGCAGT GCTTTGATCC CACCTGTACA CACATTGTTG TGGGACATCC ACTTCGAAAC
    GAGAAGTATT TAGCCTCAGT GGCAGCTGGG AAGTGGGTGC TTCATCGCTC CTACCTTGAA
    GCCTGCAGGA CTGCTGGACA CTTCGTGCAG GAAGAAGACT ATGAATGGGG AAGTAGTTCC
    ATACTTGATG TTCTGACTGG AATCAATGTA CAGCAACGAA GACTAGCACT TGCAGCAATG
    AGATGGAGAA AAAAAATCCA GCAAAGACAA GAATCTGGCA TTGTTGAGGG AGCATTTAGT
    GGGTGGAAGG TTATTTTACA TGTGGATCAG TCTCGAGAAG CAGGCTTCAA ACGCCTTCTT
    CAGTCAGGAG GAGCAAAGGT GCTACCTGGT CATTCTGTAC CTTTATTTAA AGAGGCCACA
    CATCTTTTTT CTGACTTGAA TAAACTGAAA CCAGATGACT CAGGAGTTAA TATAGCAGAA
    GCTGCTGCCC AGAACGTGTA CTGCTTGAGA ACAGAATACA TTGCTGATTA TCTCATGCAG
    GAATCACCTC CTCATGTAGA AAATTACTGT CTACCAGAAG CTATTTCATT TATTCAGAAT
    AATAAGGAAC TTGGGACTGG ATTATCACAA AAGAGGAAAG CTCCTACAGA AAAAAATAAA
    ATCAAACGAC CTAGAGTACA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu93765
    Clone ID Related Accession (Same CDS sequence) XM_017005637.1 , XM_017005637.2
    Accession Version XM_017005637.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3264bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product DNA topoisomerase 2-binding protein 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017005637.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGCTTTCTG AAGAACCATA TATCCATGCT AATTACCAGC CAGTGGAAAT TCCAGTTTCA 
    CATAAGCCTG AAAGTAAAGC AGCTCTTTTA AAAAAGAAGA ACAGCAGCTT CTCTAAGAAA
    GACTTTGCTC CTAGTGAAAA GCATGAGCAA GCTGATGAAG ATCTGCTCTC TCAATATGAA
    AATGGTAGCT CCACAGTAGT TGAGGCTAAG ACGTCTGAAG CCAGGCCCTT TAATGATTCT
    ACTCATGCTG AGCCCTTGAA TGATTCTACT CACATTTCTT TGCAAGAAGA AAACCAGTCT
    TCTGTCAGTC ATTGTGTCCC TGATGTTTCT ACAATTACTG AAGAAGGCTT ATTTAGCCAA
    AAGAGTTTCC TTGTTTTGGG TTTTAGTAAT GAAAATGAAT CTAACATCGC AAACATCATA
    AAAGAAAATG CTGGGAAAAT CATGTCCCTT CTGAGCAGAA CTGTTGCGGA TTATGCTGTG
    GTTCCTCTGC TGGGGTGTGA AGTGGAAGCC ACTGTGGGAG AAGTTGTTAC AAATACATGG
    CTGGTTACTT GCATAGACTA TCAGACTTTG TTTGATCCAA AGTCGAATCC TCTCTTCACA
    CCAGTTCCAG TAATGACAGG AATGACTCCT TTAGAGGATT GTGTTATTTC ATTTAGCCAG
    TGTGCTGGAG CAGAAAAAGA GTCTTTAACA TTCCTAGCAA ACCTCCTTGG AGCAAGTGTT
    CAAGAATACT TTGTTCGCAA ATCCAATGCA AAGAAAGGCA TGTTTGCCAG TACTCATCTT
    ATACTGAAAG AACGTGGTGG CTCTAAATAT GAAGCTGCAA AGAAGTGGAA TTTACCTGCC
    GTTACTATAG CTTGGCTGTT GGAGACTGCT AGAACGGGAA AGAGAGCAGA CGAAAGCCAT
    TTTCTGATTG AAAATTCAAC TAAAGAAGAA CGAAGTTTGG AAACAGAAAT AACAAATGGA
    ATCAATCTAA ATTCAGATAC TGCAGAGCAT CCTGGCACAC GCCTGCAAAC TCACAGAAAA
    ACCGTCGTTA CACCTTTAGA TATGAACCGC TTTCAGAGTA AAGCTTTCCG TGCTGTGGTC
    TCACAACATG CCAGACAGGT CGCAGCCTCC CCAGCAGTAG GACAACCACT TCAGAAGGAG
    CCCTCGTTAC ACCTGGATAC ACCATCAAAA TTCCTGTCCA AGGACAAACT CTTCAAGCCT
    TCCTTTGATG TGAAGGATGC ACTTGCAGCC TTGGAAACTC CAGGACGTCC CAGCCAACAG
    AAAAGGAAAC CGAGTACGCC ACTCTCAGAA GTTATTGTCA AAAACTTGCA ACTTGCTTTG
    GCAAATAGCT CTCGAAATGC TGTCGCTCTT TCTGCCAGCC CTCAACTGAA AGAGGCCCAG
    TCAGAGAAGG AAGAAGCCCC AAAGCCACTT CACAAAGTAG TGGTATGTGT TAGTAAAAAA
    CTCAGTAAGA AGCAGAGTGA ACTAAATGGG ATCGCAGCCT CTCTAGGAGC AGATTACAGG
    TGGAGTTTTG ATGAAACAGT GACTCATTTC ATCTATCAAG GGCGGCCAAA TGACACTAAT
    CGGGAGTATA AATCTGTAAA AGAAAGAGGA GTACACATTG TTTCCGAGCA CTGGCTTTTA
    GATTGTGCCC AAGAGTGTAA ACATCTTCCT GAATCTCTTT ATCCACATAC TTATAATCCC
    AAAATGAGCT TGGATATCAG CGCAGTGCAA GATGGCCGGC TCTGTAATAG TCGACTACTC
    TCAGCTGTGT CTTCAACAAA GGATGATGAG CCAGATCCTT TGATTTTAGA AGAAAATGAT
    GTAGACAATA TGGCCACCAA TAATAAAGAG TCAGCACCAT CAAATGGAAG TGGAAAGAAT
    GACTCTAAAG GAGTTCTGAC ACAGACCTTA GAGATGAGAG AGAACTTTCA GAAGCAGTTA
    CAGGAGATAA TGTCTGCAAC ATCAATAGTG AAACCCCAAG GGCAGAGGAC TTCCCTTTCA
    AGAAGTGGTT GTAACAGCGC ATCTTCAACC CCTGACAGCA CTCGCTCTGC TCGCAGTGGA
    CGAAGTAGAG TCCTAGAGGC ACTGAGGCAG TCTCGTCAGA CAGTACCTGA TGTCAACACA
    GAGCCTTCCC AAAATGAACA GATCATTTGG GATGACCCTA CAGCAAGGGA GGAGAGAGCA
    AGGCTTGCCA GCAATTTGCA GTGGCCTAGT TGTCCCACAC AATACTCTGA GCTTCAGGTT
    GACATTCAAA ACTTGGAGGA TTCTCCTTTT CAAAAGCCTT TACATGATTC AGAAATTGCT
    AAACAGGCTG TCTGTGATCC TGGAAACATA CGTGTGACTG AAGCTCCCAA ACACCCAATC
    TCTGAAGAAC TGGAAACTCC CATAAAAGAC AGCCACCTGA TCCCTACGCC TCAAGCCCCC
    AGTATTGCCT TTCCACTCGC CAACCCCCCT GTGGCTCCGC ACCCTAGAGA AAAGATTATA
    ACGATAGAGG AGACTCATGA AGAATTAAAA AAACAGTACA TATTTCAGTT ATCATCTCTG
    AATCCTCAAG AACGTATTGA CTATTGTCAT CTGATTGAGA AACTAGGTGG ATTGGTGATA
    GAAAAGCAGT GCTTTGATCC CACCTGTACA CACATTGTTG TGGGACATCC ACTTCGAAAC
    GAGAAGTATT TAGCCTCAGT GGCAGCTGGG AAGTGGGTGC TTCATCGCTC CTACCTTGAA
    GCCTGCAGGA CTGCTGGACA CTTCGTGCAG GAAGAAGACT ATGAATGGGG AAGTAGTTCC
    ATACTTGATG TTCTGACTGG AATCAATGTA CAGCAACGAA GACTAGCACT TGCAGCAATG
    AGATGGAGAA AAAAAATCCA GCAAAGACAA GAATCTGGCA TTGTTGAGGG AGCATTTAGT
    GGGTGGAAGG TTATTTTACA TGTGGATCAG TCTCGAGAAG CAGGCTTCAA ACGCCTTCTT
    CAGTCAGGAG GAGCAAAGGT GCTACCTGGT CATTCTGTAC CTTTATTTAA AGAGGCCACA
    CATCTTTTTT CTGACTTGAA TAAACTGAAA CCAGATGACT CAGGAGTTAA TATAGCAGAA
    GCTGCTGCCC AGAACGTGTA CTGCTTGAGA ACAGAATACA TTGCTGATTA TCTCATGCAG
    GAATCACCTC CTCATGTAGA AAATTACTGT CTACCAGAAG CTATTTCATT TATTCAGAAT
    AATAAGGAAC TTGGGACTGG ATTATCACAA AAGAGGAAAG CTCCTACAGA AAAAAATAAA
    ATCAAACGAC CTAGAGTACA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016861126.1
    CDS1322..4585
    Translation

    Target ORF information:

    RefSeq Version XM_017005637.2
    Organism Homo sapiens(human)
    Definition Homo sapiens DNA topoisomerase II binding protein 1 (TOPBP1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017005637.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGCTTTCTG AAGAACCATA TATCCATGCT AATTACCAGC CAGTGGAAAT TCCAGTTTCA 
    CATAAGCCTG AAAGTAAAGC AGCTCTTTTA AAAAAGAAGA ACAGCAGCTT CTCTAAGAAA
    GACTTTGCTC CTAGTGAAAA GCATGAGCAA GCTGATGAAG ATCTGCTCTC TCAATATGAA
    AATGGTAGCT CCACAGTAGT TGAGGCTAAG ACGTCTGAAG CCAGGCCCTT TAATGATTCT
    ACTCATGCTG AGCCCTTGAA TGATTCTACT CACATTTCTT TGCAAGAAGA AAACCAGTCT
    TCTGTCAGTC ATTGTGTCCC TGATGTTTCT ACAATTACTG AAGAAGGCTT ATTTAGCCAA
    AAGAGTTTCC TTGTTTTGGG TTTTAGTAAT GAAAATGAAT CTAACATCGC AAACATCATA
    AAAGAAAATG CTGGGAAAAT CATGTCCCTT CTGAGCAGAA CTGTTGCGGA TTATGCTGTG
    GTTCCTCTGC TGGGGTGTGA AGTGGAAGCC ACTGTGGGAG AAGTTGTTAC AAATACATGG
    CTGGTTACTT GCATAGACTA TCAGACTTTG TTTGATCCAA AGTCGAATCC TCTCTTCACA
    CCAGTTCCAG TAATGACAGG AATGACTCCT TTAGAGGATT GTGTTATTTC ATTTAGCCAG
    TGTGCTGGAG CAGAAAAAGA GTCTTTAACA TTCCTAGCAA ACCTCCTTGG AGCAAGTGTT
    CAAGAATACT TTGTTCGCAA ATCCAATGCA AAGAAAGGCA TGTTTGCCAG TACTCATCTT
    ATACTGAAAG AACGTGGTGG CTCTAAATAT GAAGCTGCAA AGAAGTGGAA TTTACCTGCC
    GTTACTATAG CTTGGCTGTT GGAGACTGCT AGAACGGGAA AGAGAGCAGA CGAAAGCCAT
    TTTCTGATTG AAAATTCAAC TAAAGAAGAA CGAAGTTTGG AAACAGAAAT AACAAATGGA
    ATCAATCTAA ATTCAGATAC TGCAGAGCAT CCTGGCACAC GCCTGCAAAC TCACAGAAAA
    ACCGTCGTTA CACCTTTAGA TATGAACCGC TTTCAGAGTA AAGCTTTCCG TGCTGTGGTC
    TCACAACATG CCAGACAGGT CGCAGCCTCC CCAGCAGTAG GACAACCACT TCAGAAGGAG
    CCCTCGTTAC ACCTGGATAC ACCATCAAAA TTCCTGTCCA AGGACAAACT CTTCAAGCCT
    TCCTTTGATG TGAAGGATGC ACTTGCAGCC TTGGAAACTC CAGGACGTCC CAGCCAACAG
    AAAAGGAAAC CGAGTACGCC ACTCTCAGAA GTTATTGTCA AAAACTTGCA ACTTGCTTTG
    GCAAATAGCT CTCGAAATGC TGTCGCTCTT TCTGCCAGCC CTCAACTGAA AGAGGCCCAG
    TCAGAGAAGG AAGAAGCCCC AAAGCCACTT CACAAAGTAG TGGTATGTGT TAGTAAAAAA
    CTCAGTAAGA AGCAGAGTGA ACTAAATGGG ATCGCAGCCT CTCTAGGAGC AGATTACAGG
    TGGAGTTTTG ATGAAACAGT GACTCATTTC ATCTATCAAG GGCGGCCAAA TGACACTAAT
    CGGGAGTATA AATCTGTAAA AGAAAGAGGA GTACACATTG TTTCCGAGCA CTGGCTTTTA
    GATTGTGCCC AAGAGTGTAA ACATCTTCCT GAATCTCTTT ATCCACATAC TTATAATCCC
    AAAATGAGCT TGGATATCAG CGCAGTGCAA GATGGCCGGC TCTGTAATAG TCGACTACTC
    TCAGCTGTGT CTTCAACAAA GGATGATGAG CCAGATCCTT TGATTTTAGA AGAAAATGAT
    GTAGACAATA TGGCCACCAA TAATAAAGAG TCAGCACCAT CAAATGGAAG TGGAAAGAAT
    GACTCTAAAG GAGTTCTGAC ACAGACCTTA GAGATGAGAG AGAACTTTCA GAAGCAGTTA
    CAGGAGATAA TGTCTGCAAC ATCAATAGTG AAACCCCAAG GGCAGAGGAC TTCCCTTTCA
    AGAAGTGGTT GTAACAGCGC ATCTTCAACC CCTGACAGCA CTCGCTCTGC TCGCAGTGGA
    CGAAGTAGAG TCCTAGAGGC ACTGAGGCAG TCTCGTCAGA CAGTACCTGA TGTCAACACA
    GAGCCTTCCC AAAATGAACA GATCATTTGG GATGACCCTA CAGCAAGGGA GGAGAGAGCA
    AGGCTTGCCA GCAATTTGCA GTGGCCTAGT TGTCCCACAC AATACTCTGA GCTTCAGGTT
    GACATTCAAA ACTTGGAGGA TTCTCCTTTT CAAAAGCCTT TACATGATTC AGAAATTGCT
    AAACAGGCTG TCTGTGATCC TGGAAACATA CGTGTGACTG AAGCTCCCAA ACACCCAATC
    TCTGAAGAAC TGGAAACTCC CATAAAAGAC AGCCACCTGA TCCCTACGCC TCAAGCCCCC
    AGTATTGCCT TTCCACTCGC CAACCCCCCT GTGGCTCCGC ACCCTAGAGA AAAGATTATA
    ACGATAGAGG AGACTCATGA AGAATTAAAA AAACAGTACA TATTTCAGTT ATCATCTCTG
    AATCCTCAAG AACGTATTGA CTATTGTCAT CTGATTGAGA AACTAGGTGG ATTGGTGATA
    GAAAAGCAGT GCTTTGATCC CACCTGTACA CACATTGTTG TGGGACATCC ACTTCGAAAC
    GAGAAGTATT TAGCCTCAGT GGCAGCTGGG AAGTGGGTGC TTCATCGCTC CTACCTTGAA
    GCCTGCAGGA CTGCTGGACA CTTCGTGCAG GAAGAAGACT ATGAATGGGG AAGTAGTTCC
    ATACTTGATG TTCTGACTGG AATCAATGTA CAGCAACGAA GACTAGCACT TGCAGCAATG
    AGATGGAGAA AAAAAATCCA GCAAAGACAA GAATCTGGCA TTGTTGAGGG AGCATTTAGT
    GGGTGGAAGG TTATTTTACA TGTGGATCAG TCTCGAGAAG CAGGCTTCAA ACGCCTTCTT
    CAGTCAGGAG GAGCAAAGGT GCTACCTGGT CATTCTGTAC CTTTATTTAA AGAGGCCACA
    CATCTTTTTT CTGACTTGAA TAAACTGAAA CCAGATGACT CAGGAGTTAA TATAGCAGAA
    GCTGCTGCCC AGAACGTGTA CTGCTTGAGA ACAGAATACA TTGCTGATTA TCTCATGCAG
    GAATCACCTC CTCATGTAGA AAATTACTGT CTACCAGAAG CTATTTCATT TATTCAGAAT
    AATAAGGAAC TTGGGACTGG ATTATCACAA AAGAGGAAAG CTCCTACAGA AAAAAATAAA
    ATCAAACGAC CTAGAGTACA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu64678
    Clone ID Related Accession (Same CDS sequence) XM_011512357.3 , XM_011512357.2
    Accession Version XM_011512357.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3381bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product DNA topoisomerase 2-binding protein 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011512357.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGTCCAGAA ATGACAAAGA ACCGTTTTTT GTGAAGTTTT TAAAGTCTTC AGACAATTCC 
    AAATGTTTTT TTAAAGCTCT CGAGTCCATA AAAGAATTCC AATCAGAAGA ATATCTTCAG
    ATTATTACAG AAGAAGAGGC ATTGAAGATA AAGGAGAATG ATAGATCACT TTATATCTGT
    GACCCTTTTA GTGGCGTTGT CTTTGATCAC CTCAAAAAGC TTGGCTGCAG AATTGTTGGT
    CCTCAAGTAG TCATATTTTG TATGCACCAC CAGCGATGTG TCCCAAGAGC CGAACATCCA
    GTTTATAATA TGGTTATGTC TGATGTAACC ATATCTTGTA CAAGTCTGGA AAAAGAAAAA
    AGGGAAGAAG TTCATAAATA TGTACAAATG ATGGGCGGAC GAGTATACAG AGACCTTAAT
    GTATCAGTAA CTCACCTTAT TGCAGGAGAA GTTGGTAGCA AAAAATATTT AGTTGCTGCA
    AACCTGAAGA AACCTATTTT GCTTCCCTCT TGGATAAAAA CACTTTGGGA GAAGTCACAA
    GAGAAAAAAA TAACTAGATA TACTGATATA AACATGGAAG ATTTCAAGTG TCCTATTTTT
    CTTGGTTGCA TAATCTGTGT GACTGGCTTA TGTGGCTTAG ACAGGAAAGA AGTTCAGCAA
    CTCACAGTTA AGCATGGAGG TCAATACATG GGACAATTGA AAATGAATGA ATGTACACAC
    CTCATTGTGC AAGAACCAAA AGGTCAGAAG TATGAGTGTG CCAAGAGATG GAATGTACAC
    TGTGTGACCA CACAGTGGTT TTTTGACAGT ATTGAGAAAG GTTTTTGTCA GGATGAATCC
    ATATACAAGA CAGAACCTAG ACCAGAAGCA AAGACTATGC CCAATTCTTC AACTCCTACC
    AGCCAGATCA ACACAATTGA TAGTCGTACT CTTTCAGATG TCAGCAATAT TTCCAACATA
    AATGCAAGTT GCGTAAGTGA ATCAATATGT AATTCACTTA ACAGCAAACT GGAGCCTACA
    CTTGAAAATC TAGAAAATCT GGATGTCAGT GCATTTCAAG CACCTGAAGA TTTATTAGAT
    GGTTGTCGGA TATATCTTTG CGGTTTTAGT GGCAGAAAGC TAGATAAACT GAGAAGACTT
    ATTAACAGTG GAGGTGGAGT TCGTTTTAAC CAGCTAAATG AAGATGTAAC TCATGTTATT
    GTGGGAGATT ATGATGATGA ATTGAAGCAG TTTTGGAATA AATCAGCCCA CAGGCCTCAT
    GTAGTGGGAG CAAAGTGGTT GCTAGAGTGT TTCAGTAAAG GTTATATGCT TTCTGAAGAA
    CCATATATCC ATGCTAATTA CCAGCCAGTG GAAATTCCAG TTTCACATAA GCCTGAAAGT
    AAAGCAGCTC TTTTAAAAAA GAAGAACAGC AGCTTCTCTA AGAAAGACTT TGCTCCTAGT
    GAAAAGCATG AGCAAGCTGA TGAAGATCTG CTCTCTCAAT ATGAAAATGG TAGCTCCACA
    GTAGTTGAGG CTAAGACGTC TGAAGCCAGG CCCTTTAATG ATTCTACTCA TGCTGAGCCC
    TTGAATGATT CTACTCACAT TTCTTTGCAA GAAGAAAACC AGTCTTCTGT CAGTCATTGT
    GTCCCTGATG TTTCTACAAT TACTGAAGAA GGCTTATTTA GCCAAAAGAG TTTCCTTGTT
    TTGGGTTTTA GTAATGAAAA TGAATCTAAC ATCGCAAACA TCATAAAAGA AAATGCTGGG
    AAAATCATGT CCCTTCTGAG CAGAACTGTT GCGGATTATG CTGTGGTTCC TCTGCTGGGG
    TGTGAAGTGG AAGCCACTGT GGGAGAAGTT GTTACAAATA CATGGCTGGT TACTTGCATA
    GACTATCAGA CTTTGTTTGA TCCAAAGTCG AATCCTCTCT TCACACCAGT TCCAGTAATG
    ACAGGAATGA CTCCTTTAGA GGATTGTGTT ATTTCATTTA GCCAGTGTGC TGGAGCAGAA
    AAAGAGTCTT TAACATTCCT AGCAAACCTC CTTGGAGCAA GTGTTCAAGA ATACTTTGTT
    CGCAAATCCA ATGCAAAGAA AGGCATGTTT GCCAGTACTC ATCTTATACT GAAAGAACGT
    GGTGGCTCTA AATATGAAGC TGCAAAGAAG TGGAATTTAC CTGCCGTTAC TATAGCTTGG
    CTGTTGGAGA CTGCTAGAAC GGGAAAGAGA GCAGACGAAA GCCATTTTCT GATTGAAAAT
    TCAACTAAAG AAGAACGAAG TTTGGAAACA GAAATAACAA ATGGAATCAA TCTAAATTCA
    GATACTGCAG AGCATCCTGG CACACGCCTG CAAACTCACA GAAAAACCGT CGTTACACCT
    TTAGATATGA ACCGCTTTCA GAGTAAAGCT TTCCGTGCTG TGGTCTCACA ACATGCCAGA
    CAGGTCGCAG CCTCCCCAGC AGTAGGACAA CCACTTCAGA AGGAGCCCTC GTTACACCTG
    GATACACCAT CAAAATTCCT GTCCAAGGAC AAACTCTTCA AGCCTTCCTT TGATGTGAAG
    GATGCACTTG CAGCCTTGGA AACTCCAGGA CGTCCCAGCC AACAGAAAAG GAAACCGAGT
    ACGCCACTCT CAGAAGTTAT TGTCAAAAAC TTGCAACTTG CTTTGGCAAA TAGCTCTCGA
    AATGCTGTCG CTCTTTCTGC CAGCCCTCAA CTGAAAGAGG CCCAGTCAGA GAAGGAAGAA
    GCCCCAAAGC CACTTCACAA AGTAGTGGTA TGTGTTAGTA AAAAACTCAG TAAGAAGCAG
    AGTGAACTAA ATGGGATCGC AGCCTCTCTA GGAGCAGATT ACAGGTGGAG TTTTGATGAA
    ACAGTGACTC ATTTCATCTA TCAAGGGCGG CCAAATGACA CTAATCGGGA GTATAAATCT
    GTAAAAGAAA GAGGAGTACA CATTGTTTCC GAGCACTGGC TTTTAGATTG TGCCCAAGAG
    TGTAAACATC TTCCTGAATC TCTTTATCCA CATACTTATA ATCCCAAAAT GAGCTTGGAT
    ATCAGCGCAG TGCAAGATGG CCGGCTCTGT AATAGTCGAC TACTCTCAGC TGTGTCTTCA
    ACAAAGGATG ATGAGCCAGA TCCTTTGATT TTAGAAGAAA ATGATGTAGA CAATATGGCC
    ACCAATAATA AAGAGTCAGC ACCATCAAAT GGAAGTGGAA AGAATGACTC TAAAGGAGTT
    CTGACACAGA CCTTAGAGAT GAGAGAGAAC TTTCAGAAGC AGTTACAGGA GATAATGTCT
    GCAACATCAA TAGTGAAACC CCAAGGGCAG AGGACTTCCC TTTCAAGAAG TGGTTGTAAC
    AGCGCATCTT CAACCCCTGA CAGCACTCGC TCTGCTCGCA GTGGACGAAG TAGAGTCCTA
    GAGGCACTGA GCTGTCTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011510659.1
    CDS99..3479
    Translation

    Target ORF information:

    RefSeq Version XM_011512357.3
    Organism Homo sapiens(human)
    Definition Homo sapiens DNA topoisomerase II binding protein 1 (TOPBP1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011512357.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGTCCAGAA ATGACAAAGA ACCGTTTTTT GTGAAGTTTT TAAAGTCTTC AGACAATTCC 
    AAATGTTTTT TTAAAGCTCT CGAGTCCATA AAAGAATTCC AATCAGAAGA ATATCTTCAG
    ATTATTACAG AAGAAGAGGC ATTGAAGATA AAGGAGAATG ATAGATCACT TTATATCTGT
    GACCCTTTTA GTGGCGTTGT CTTTGATCAC CTCAAAAAGC TTGGCTGCAG AATTGTTGGT
    CCTCAAGTAG TCATATTTTG TATGCACCAC CAGCGATGTG TCCCAAGAGC CGAACATCCA
    GTTTATAATA TGGTTATGTC TGATGTAACC ATATCTTGTA CAAGTCTGGA AAAAGAAAAA
    AGGGAAGAAG TTCATAAATA TGTACAAATG ATGGGCGGAC GAGTATACAG AGACCTTAAT
    GTATCAGTAA CTCACCTTAT TGCAGGAGAA GTTGGTAGCA AAAAATATTT AGTTGCTGCA
    AACCTGAAGA AACCTATTTT GCTTCCCTCT TGGATAAAAA CACTTTGGGA GAAGTCACAA
    GAGAAAAAAA TAACTAGATA TACTGATATA AACATGGAAG ATTTCAAGTG TCCTATTTTT
    CTTGGTTGCA TAATCTGTGT GACTGGCTTA TGTGGCTTAG ACAGGAAAGA AGTTCAGCAA
    CTCACAGTTA AGCATGGAGG TCAATACATG GGACAATTGA AAATGAATGA ATGTACACAC
    CTCATTGTGC AAGAACCAAA AGGTCAGAAG TATGAGTGTG CCAAGAGATG GAATGTACAC
    TGTGTGACCA CACAGTGGTT TTTTGACAGT ATTGAGAAAG GTTTTTGTCA GGATGAATCC
    ATATACAAGA CAGAACCTAG ACCAGAAGCA AAGACTATGC CCAATTCTTC AACTCCTACC
    AGCCAGATCA ACACAATTGA TAGTCGTACT CTTTCAGATG TCAGCAATAT TTCCAACATA
    AATGCAAGTT GCGTAAGTGA ATCAATATGT AATTCACTTA ACAGCAAACT GGAGCCTACA
    CTTGAAAATC TAGAAAATCT GGATGTCAGT GCATTTCAAG CACCTGAAGA TTTATTAGAT
    GGTTGTCGGA TATATCTTTG CGGTTTTAGT GGCAGAAAGC TAGATAAACT GAGAAGACTT
    ATTAACAGTG GAGGTGGAGT TCGTTTTAAC CAGCTAAATG AAGATGTAAC TCATGTTATT
    GTGGGAGATT ATGATGATGA ATTGAAGCAG TTTTGGAATA AATCAGCCCA CAGGCCTCAT
    GTAGTGGGAG CAAAGTGGTT GCTAGAGTGT TTCAGTAAAG GTTATATGCT TTCTGAAGAA
    CCATATATCC ATGCTAATTA CCAGCCAGTG GAAATTCCAG TTTCACATAA GCCTGAAAGT
    AAAGCAGCTC TTTTAAAAAA GAAGAACAGC AGCTTCTCTA AGAAAGACTT TGCTCCTAGT
    GAAAAGCATG AGCAAGCTGA TGAAGATCTG CTCTCTCAAT ATGAAAATGG TAGCTCCACA
    GTAGTTGAGG CTAAGACGTC TGAAGCCAGG CCCTTTAATG ATTCTACTCA TGCTGAGCCC
    TTGAATGATT CTACTCACAT TTCTTTGCAA GAAGAAAACC AGTCTTCTGT CAGTCATTGT
    GTCCCTGATG TTTCTACAAT TACTGAAGAA GGCTTATTTA GCCAAAAGAG TTTCCTTGTT
    TTGGGTTTTA GTAATGAAAA TGAATCTAAC ATCGCAAACA TCATAAAAGA AAATGCTGGG
    AAAATCATGT CCCTTCTGAG CAGAACTGTT GCGGATTATG CTGTGGTTCC TCTGCTGGGG
    TGTGAAGTGG AAGCCACTGT GGGAGAAGTT GTTACAAATA CATGGCTGGT TACTTGCATA
    GACTATCAGA CTTTGTTTGA TCCAAAGTCG AATCCTCTCT TCACACCAGT TCCAGTAATG
    ACAGGAATGA CTCCTTTAGA GGATTGTGTT ATTTCATTTA GCCAGTGTGC TGGAGCAGAA
    AAAGAGTCTT TAACATTCCT AGCAAACCTC CTTGGAGCAA GTGTTCAAGA ATACTTTGTT
    CGCAAATCCA ATGCAAAGAA AGGCATGTTT GCCAGTACTC ATCTTATACT GAAAGAACGT
    GGTGGCTCTA AATATGAAGC TGCAAAGAAG TGGAATTTAC CTGCCGTTAC TATAGCTTGG
    CTGTTGGAGA CTGCTAGAAC GGGAAAGAGA GCAGACGAAA GCCATTTTCT GATTGAAAAT
    TCAACTAAAG AAGAACGAAG TTTGGAAACA GAAATAACAA ATGGAATCAA TCTAAATTCA
    GATACTGCAG AGCATCCTGG CACACGCCTG CAAACTCACA GAAAAACCGT CGTTACACCT
    TTAGATATGA ACCGCTTTCA GAGTAAAGCT TTCCGTGCTG TGGTCTCACA ACATGCCAGA
    CAGGTCGCAG CCTCCCCAGC AGTAGGACAA CCACTTCAGA AGGAGCCCTC GTTACACCTG
    GATACACCAT CAAAATTCCT GTCCAAGGAC AAACTCTTCA AGCCTTCCTT TGATGTGAAG
    GATGCACTTG CAGCCTTGGA AACTCCAGGA CGTCCCAGCC AACAGAAAAG GAAACCGAGT
    ACGCCACTCT CAGAAGTTAT TGTCAAAAAC TTGCAACTTG CTTTGGCAAA TAGCTCTCGA
    AATGCTGTCG CTCTTTCTGC CAGCCCTCAA CTGAAAGAGG CCCAGTCAGA GAAGGAAGAA
    GCCCCAAAGC CACTTCACAA AGTAGTGGTA TGTGTTAGTA AAAAACTCAG TAAGAAGCAG
    AGTGAACTAA ATGGGATCGC AGCCTCTCTA GGAGCAGATT ACAGGTGGAG TTTTGATGAA
    ACAGTGACTC ATTTCATCTA TCAAGGGCGG CCAAATGACA CTAATCGGGA GTATAAATCT
    GTAAAAGAAA GAGGAGTACA CATTGTTTCC GAGCACTGGC TTTTAGATTG TGCCCAAGAG
    TGTAAACATC TTCCTGAATC TCTTTATCCA CATACTTATA ATCCCAAAAT GAGCTTGGAT
    ATCAGCGCAG TGCAAGATGG CCGGCTCTGT AATAGTCGAC TACTCTCAGC TGTGTCTTCA
    ACAAAGGATG ATGAGCCAGA TCCTTTGATT TTAGAAGAAA ATGATGTAGA CAATATGGCC
    ACCAATAATA AAGAGTCAGC ACCATCAAAT GGAAGTGGAA AGAATGACTC TAAAGGAGTT
    CTGACACAGA CCTTAGAGAT GAGAGAGAAC TTTCAGAAGC AGTTACAGGA GATAATGTCT
    GCAACATCAA TAGTGAAACC CCAAGGGCAG AGGACTTCCC TTTCAAGAAG TGGTTGTAAC
    AGCGCATCTT CAACCCCTGA CAGCACTCGC TCTGCTCGCA GTGGACGAAG TAGAGTCCTA
    GAGGCACTGA GCTGTCTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu64678
    Clone ID Related Accession (Same CDS sequence) XM_011512357.3 , XM_011512357.2
    Accession Version XM_011512357.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3381bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product DNA topoisomerase 2-binding protein 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011512357.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGTCCAGAA ATGACAAAGA ACCGTTTTTT GTGAAGTTTT TAAAGTCTTC AGACAATTCC 
    AAATGTTTTT TTAAAGCTCT CGAGTCCATA AAAGAATTCC AATCAGAAGA ATATCTTCAG
    ATTATTACAG AAGAAGAGGC ATTGAAGATA AAGGAGAATG ATAGATCACT TTATATCTGT
    GACCCTTTTA GTGGCGTTGT CTTTGATCAC CTCAAAAAGC TTGGCTGCAG AATTGTTGGT
    CCTCAAGTAG TCATATTTTG TATGCACCAC CAGCGATGTG TCCCAAGAGC CGAACATCCA
    GTTTATAATA TGGTTATGTC TGATGTAACC ATATCTTGTA CAAGTCTGGA AAAAGAAAAA
    AGGGAAGAAG TTCATAAATA TGTACAAATG ATGGGCGGAC GAGTATACAG AGACCTTAAT
    GTATCAGTAA CTCACCTTAT TGCAGGAGAA GTTGGTAGCA AAAAATATTT AGTTGCTGCA
    AACCTGAAGA AACCTATTTT GCTTCCCTCT TGGATAAAAA CACTTTGGGA GAAGTCACAA
    GAGAAAAAAA TAACTAGATA TACTGATATA AACATGGAAG ATTTCAAGTG TCCTATTTTT
    CTTGGTTGCA TAATCTGTGT GACTGGCTTA TGTGGCTTAG ACAGGAAAGA AGTTCAGCAA
    CTCACAGTTA AGCATGGAGG TCAATACATG GGACAATTGA AAATGAATGA ATGTACACAC
    CTCATTGTGC AAGAACCAAA AGGTCAGAAG TATGAGTGTG CCAAGAGATG GAATGTACAC
    TGTGTGACCA CACAGTGGTT TTTTGACAGT ATTGAGAAAG GTTTTTGTCA GGATGAATCC
    ATATACAAGA CAGAACCTAG ACCAGAAGCA AAGACTATGC CCAATTCTTC AACTCCTACC
    AGCCAGATCA ACACAATTGA TAGTCGTACT CTTTCAGATG TCAGCAATAT TTCCAACATA
    AATGCAAGTT GCGTAAGTGA ATCAATATGT AATTCACTTA ACAGCAAACT GGAGCCTACA
    CTTGAAAATC TAGAAAATCT GGATGTCAGT GCATTTCAAG CACCTGAAGA TTTATTAGAT
    GGTTGTCGGA TATATCTTTG CGGTTTTAGT GGCAGAAAGC TAGATAAACT GAGAAGACTT
    ATTAACAGTG GAGGTGGAGT TCGTTTTAAC CAGCTAAATG AAGATGTAAC TCATGTTATT
    GTGGGAGATT ATGATGATGA ATTGAAGCAG TTTTGGAATA AATCAGCCCA CAGGCCTCAT
    GTAGTGGGAG CAAAGTGGTT GCTAGAGTGT TTCAGTAAAG GTTATATGCT TTCTGAAGAA
    CCATATATCC ATGCTAATTA CCAGCCAGTG GAAATTCCAG TTTCACATAA GCCTGAAAGT
    AAAGCAGCTC TTTTAAAAAA GAAGAACAGC AGCTTCTCTA AGAAAGACTT TGCTCCTAGT
    GAAAAGCATG AGCAAGCTGA TGAAGATCTG CTCTCTCAAT ATGAAAATGG TAGCTCCACA
    GTAGTTGAGG CTAAGACGTC TGAAGCCAGG CCCTTTAATG ATTCTACTCA TGCTGAGCCC
    TTGAATGATT CTACTCACAT TTCTTTGCAA GAAGAAAACC AGTCTTCTGT CAGTCATTGT
    GTCCCTGATG TTTCTACAAT TACTGAAGAA GGCTTATTTA GCCAAAAGAG TTTCCTTGTT
    TTGGGTTTTA GTAATGAAAA TGAATCTAAC ATCGCAAACA TCATAAAAGA AAATGCTGGG
    AAAATCATGT CCCTTCTGAG CAGAACTGTT GCGGATTATG CTGTGGTTCC TCTGCTGGGG
    TGTGAAGTGG AAGCCACTGT GGGAGAAGTT GTTACAAATA CATGGCTGGT TACTTGCATA
    GACTATCAGA CTTTGTTTGA TCCAAAGTCG AATCCTCTCT TCACACCAGT TCCAGTAATG
    ACAGGAATGA CTCCTTTAGA GGATTGTGTT ATTTCATTTA GCCAGTGTGC TGGAGCAGAA
    AAAGAGTCTT TAACATTCCT AGCAAACCTC CTTGGAGCAA GTGTTCAAGA ATACTTTGTT
    CGCAAATCCA ATGCAAAGAA AGGCATGTTT GCCAGTACTC ATCTTATACT GAAAGAACGT
    GGTGGCTCTA AATATGAAGC TGCAAAGAAG TGGAATTTAC CTGCCGTTAC TATAGCTTGG
    CTGTTGGAGA CTGCTAGAAC GGGAAAGAGA GCAGACGAAA GCCATTTTCT GATTGAAAAT
    TCAACTAAAG AAGAACGAAG TTTGGAAACA GAAATAACAA ATGGAATCAA TCTAAATTCA
    GATACTGCAG AGCATCCTGG CACACGCCTG CAAACTCACA GAAAAACCGT CGTTACACCT
    TTAGATATGA ACCGCTTTCA GAGTAAAGCT TTCCGTGCTG TGGTCTCACA ACATGCCAGA
    CAGGTCGCAG CCTCCCCAGC AGTAGGACAA CCACTTCAGA AGGAGCCCTC GTTACACCTG
    GATACACCAT CAAAATTCCT GTCCAAGGAC AAACTCTTCA AGCCTTCCTT TGATGTGAAG
    GATGCACTTG CAGCCTTGGA AACTCCAGGA CGTCCCAGCC AACAGAAAAG GAAACCGAGT
    ACGCCACTCT CAGAAGTTAT TGTCAAAAAC TTGCAACTTG CTTTGGCAAA TAGCTCTCGA
    AATGCTGTCG CTCTTTCTGC CAGCCCTCAA CTGAAAGAGG CCCAGTCAGA GAAGGAAGAA
    GCCCCAAAGC CACTTCACAA AGTAGTGGTA TGTGTTAGTA AAAAACTCAG TAAGAAGCAG
    AGTGAACTAA ATGGGATCGC AGCCTCTCTA GGAGCAGATT ACAGGTGGAG TTTTGATGAA
    ACAGTGACTC ATTTCATCTA TCAAGGGCGG CCAAATGACA CTAATCGGGA GTATAAATCT
    GTAAAAGAAA GAGGAGTACA CATTGTTTCC GAGCACTGGC TTTTAGATTG TGCCCAAGAG
    TGTAAACATC TTCCTGAATC TCTTTATCCA CATACTTATA ATCCCAAAAT GAGCTTGGAT
    ATCAGCGCAG TGCAAGATGG CCGGCTCTGT AATAGTCGAC TACTCTCAGC TGTGTCTTCA
    ACAAAGGATG ATGAGCCAGA TCCTTTGATT TTAGAAGAAA ATGATGTAGA CAATATGGCC
    ACCAATAATA AAGAGTCAGC ACCATCAAAT GGAAGTGGAA AGAATGACTC TAAAGGAGTT
    CTGACACAGA CCTTAGAGAT GAGAGAGAAC TTTCAGAAGC AGTTACAGGA GATAATGTCT
    GCAACATCAA TAGTGAAACC CCAAGGGCAG AGGACTTCCC TTTCAAGAAG TGGTTGTAAC
    AGCGCATCTT CAACCCCTGA CAGCACTCGC TCTGCTCGCA GTGGACGAAG TAGAGTCCTA
    GAGGCACTGA GCTGTCTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011510659.1
    CDS204..3584
    Translation

    Target ORF information:

    RefSeq Version XM_011512357.2
    Organism Homo sapiens(human)
    Definition Homo sapiens topoisomerase (DNA) II binding protein 1 (TOPBP1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011512357.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGTCCAGAA ATGACAAAGA ACCGTTTTTT GTGAAGTTTT TAAAGTCTTC AGACAATTCC 
    AAATGTTTTT TTAAAGCTCT CGAGTCCATA AAAGAATTCC AATCAGAAGA ATATCTTCAG
    ATTATTACAG AAGAAGAGGC ATTGAAGATA AAGGAGAATG ATAGATCACT TTATATCTGT
    GACCCTTTTA GTGGCGTTGT CTTTGATCAC CTCAAAAAGC TTGGCTGCAG AATTGTTGGT
    CCTCAAGTAG TCATATTTTG TATGCACCAC CAGCGATGTG TCCCAAGAGC CGAACATCCA
    GTTTATAATA TGGTTATGTC TGATGTAACC ATATCTTGTA CAAGTCTGGA AAAAGAAAAA
    AGGGAAGAAG TTCATAAATA TGTACAAATG ATGGGCGGAC GAGTATACAG AGACCTTAAT
    GTATCAGTAA CTCACCTTAT TGCAGGAGAA GTTGGTAGCA AAAAATATTT AGTTGCTGCA
    AACCTGAAGA AACCTATTTT GCTTCCCTCT TGGATAAAAA CACTTTGGGA GAAGTCACAA
    GAGAAAAAAA TAACTAGATA TACTGATATA AACATGGAAG ATTTCAAGTG TCCTATTTTT
    CTTGGTTGCA TAATCTGTGT GACTGGCTTA TGTGGCTTAG ACAGGAAAGA AGTTCAGCAA
    CTCACAGTTA AGCATGGAGG TCAATACATG GGACAATTGA AAATGAATGA ATGTACACAC
    CTCATTGTGC AAGAACCAAA AGGTCAGAAG TATGAGTGTG CCAAGAGATG GAATGTACAC
    TGTGTGACCA CACAGTGGTT TTTTGACAGT ATTGAGAAAG GTTTTTGTCA GGATGAATCC
    ATATACAAGA CAGAACCTAG ACCAGAAGCA AAGACTATGC CCAATTCTTC AACTCCTACC
    AGCCAGATCA ACACAATTGA TAGTCGTACT CTTTCAGATG TCAGCAATAT TTCCAACATA
    AATGCAAGTT GCGTAAGTGA ATCAATATGT AATTCACTTA ACAGCAAACT GGAGCCTACA
    CTTGAAAATC TAGAAAATCT GGATGTCAGT GCATTTCAAG CACCTGAAGA TTTATTAGAT
    GGTTGTCGGA TATATCTTTG CGGTTTTAGT GGCAGAAAGC TAGATAAACT GAGAAGACTT
    ATTAACAGTG GAGGTGGAGT TCGTTTTAAC CAGCTAAATG AAGATGTAAC TCATGTTATT
    GTGGGAGATT ATGATGATGA ATTGAAGCAG TTTTGGAATA AATCAGCCCA CAGGCCTCAT
    GTAGTGGGAG CAAAGTGGTT GCTAGAGTGT TTCAGTAAAG GTTATATGCT TTCTGAAGAA
    CCATATATCC ATGCTAATTA CCAGCCAGTG GAAATTCCAG TTTCACATAA GCCTGAAAGT
    AAAGCAGCTC TTTTAAAAAA GAAGAACAGC AGCTTCTCTA AGAAAGACTT TGCTCCTAGT
    GAAAAGCATG AGCAAGCTGA TGAAGATCTG CTCTCTCAAT ATGAAAATGG TAGCTCCACA
    GTAGTTGAGG CTAAGACGTC TGAAGCCAGG CCCTTTAATG ATTCTACTCA TGCTGAGCCC
    TTGAATGATT CTACTCACAT TTCTTTGCAA GAAGAAAACC AGTCTTCTGT CAGTCATTGT
    GTCCCTGATG TTTCTACAAT TACTGAAGAA GGCTTATTTA GCCAAAAGAG TTTCCTTGTT
    TTGGGTTTTA GTAATGAAAA TGAATCTAAC ATCGCAAACA TCATAAAAGA AAATGCTGGG
    AAAATCATGT CCCTTCTGAG CAGAACTGTT GCGGATTATG CTGTGGTTCC TCTGCTGGGG
    TGTGAAGTGG AAGCCACTGT GGGAGAAGTT GTTACAAATA CATGGCTGGT TACTTGCATA
    GACTATCAGA CTTTGTTTGA TCCAAAGTCG AATCCTCTCT TCACACCAGT TCCAGTAATG
    ACAGGAATGA CTCCTTTAGA GGATTGTGTT ATTTCATTTA GCCAGTGTGC TGGAGCAGAA
    AAAGAGTCTT TAACATTCCT AGCAAACCTC CTTGGAGCAA GTGTTCAAGA ATACTTTGTT
    CGCAAATCCA ATGCAAAGAA AGGCATGTTT GCCAGTACTC ATCTTATACT GAAAGAACGT
    GGTGGCTCTA AATATGAAGC TGCAAAGAAG TGGAATTTAC CTGCCGTTAC TATAGCTTGG
    CTGTTGGAGA CTGCTAGAAC GGGAAAGAGA GCAGACGAAA GCCATTTTCT GATTGAAAAT
    TCAACTAAAG AAGAACGAAG TTTGGAAACA GAAATAACAA ATGGAATCAA TCTAAATTCA
    GATACTGCAG AGCATCCTGG CACACGCCTG CAAACTCACA GAAAAACCGT CGTTACACCT
    TTAGATATGA ACCGCTTTCA GAGTAAAGCT TTCCGTGCTG TGGTCTCACA ACATGCCAGA
    CAGGTCGCAG CCTCCCCAGC AGTAGGACAA CCACTTCAGA AGGAGCCCTC GTTACACCTG
    GATACACCAT CAAAATTCCT GTCCAAGGAC AAACTCTTCA AGCCTTCCTT TGATGTGAAG
    GATGCACTTG CAGCCTTGGA AACTCCAGGA CGTCCCAGCC AACAGAAAAG GAAACCGAGT
    ACGCCACTCT CAGAAGTTAT TGTCAAAAAC TTGCAACTTG CTTTGGCAAA TAGCTCTCGA
    AATGCTGTCG CTCTTTCTGC CAGCCCTCAA CTGAAAGAGG CCCAGTCAGA GAAGGAAGAA
    GCCCCAAAGC CACTTCACAA AGTAGTGGTA TGTGTTAGTA AAAAACTCAG TAAGAAGCAG
    AGTGAACTAA ATGGGATCGC AGCCTCTCTA GGAGCAGATT ACAGGTGGAG TTTTGATGAA
    ACAGTGACTC ATTTCATCTA TCAAGGGCGG CCAAATGACA CTAATCGGGA GTATAAATCT
    GTAAAAGAAA GAGGAGTACA CATTGTTTCC GAGCACTGGC TTTTAGATTG TGCCCAAGAG
    TGTAAACATC TTCCTGAATC TCTTTATCCA CATACTTATA ATCCCAAAAT GAGCTTGGAT
    ATCAGCGCAG TGCAAGATGG CCGGCTCTGT AATAGTCGAC TACTCTCAGC TGTGTCTTCA
    ACAAAGGATG ATGAGCCAGA TCCTTTGATT TTAGAAGAAA ATGATGTAGA CAATATGGCC
    ACCAATAATA AAGAGTCAGC ACCATCAAAT GGAAGTGGAA AGAATGACTC TAAAGGAGTT
    CTGACACAGA CCTTAGAGAT GAGAGAGAAC TTTCAGAAGC AGTTACAGGA GATAATGTCT
    GCAACATCAA TAGTGAAACC CCAAGGGCAG AGGACTTCCC TTTCAAGAAG TGGTTGTAAC
    AGCGCATCTT CAACCCCTGA CAGCACTCGC TCTGCTCGCA GTGGACGAAG TAGAGTCCTA
    GAGGCACTGA GCTGTCTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu37681
    Clone ID Related Accession (Same CDS sequence) NM_001363889.2 , XM_005247076.3 , NM_001363889.1
    Accession Version NM_001363889.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4554bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-15
    Organism Homo sapiens(human)
    Product DNA topoisomerase 2-binding protein 1 isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC083905.20 and AC016255.24. On May 31, 2019 this sequence version replaced NM_001363889.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.22551.1, SRR1660807.143600.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTCCAGAA ATGACAAAGA ACCGTTTTTT GTGAAGTTTT TAAAGTCTTC AGACAATTCC 
    AAATGTTTTT TTAAAGCTCT CGAGTCCATA AAAGAATTCC AATCAGAAGA ATATCTTCAG
    ATTATTACAG AAGAAGAGGC ATTGAAGATA AAGGAGAATG ATAGATCACT TTATATCTGT
    GACCCTTTTA GTGGCGTTGT CTTTGATCAC CTCAAAAAGC TTGGCTGCAG AATTGTTGGT
    CCTCAAGTAG TCATATTTTG TATGCACCAC CAGCGATGTG TCCCAAGAGC CGAACATCCA
    GTTTATAATA TGGTTATGTC TGATGTAACC ATATCTTGTA CAAGTCTGGA AAAAGAAAAA
    AGGGAAGAAG TTCATAAATA TGTACAAATG ATGGGCGGAC GAGTATACAG AGACCTTAAT
    GTATCAGTAA CTCACCTTAT TGCAGGAGAA GTTGGTAGCA AAAAATATTT AGTTGCTGCA
    AACCTGAAGA AACCTATTTT GCTTCCCTCT TGGATAAAAA CACTTTGGGA GAAGTCACAA
    GAGAAAAAAA TAACTAGATA TACTGATATA AACATGGAAG ATTTCAAGTG TCCTATTTTT
    CTTGGTTGCA TAATCTGTGT GACTGGCTTA TGTGGCTTAG ACAGGAAAGA AGTTCAGCAA
    CTCACAGTTA AGCATGGAGG TCAATACATG GGACAATTGA AAATGAATGA ATGTACACAC
    CTCATTGTGC AAGAACCAAA AGGTCAGAAG TATGAGTGTG CCAAGAGATG GAATGTACAC
    TGTGTGACCA CACAGTGGTT TTTTGACAGT ATTGAGAAAG GTTTTTGTCA GGATGAATCC
    ATATACAAGA CAGAACCTAG ACCAGAAGCA AAGACTATGC CCAATTCTTC AACTCCTACC
    AGCCAGATCA ACACAATTGA TAATGTCAGC AATATTTCCA ACATAAATGC AAGTTGCGTA
    AGTGAATCAA TATGTAATTC ACTTAACAGC AAACTGGAGC CTACACTTGA AAATCTAGAA
    AATCTGGATG TCAGTGCATT TCAAGCACCT GAAGATTTAT TAGATGGTTG TCGGATATAT
    CTTTGCGGTT TTAGTGGCAG AAAGCTAGAT AAACTGAGAA GACTTATTAA CAGTGGAGGT
    GGAGTTCGTT TTAACCAGCT AAATGAAGAT GTAACTCATG TTATTGTGGG AGATTATGAT
    GATGAATTGA AGCAGTTTTG GAATAAATCA GCCCACAGGC CTCATGTAGT GGGAGCAAAG
    TGGTTGCTAG AGTGTTTCAG TAAAGGTTAT ATGCTTTCTG AAGAACCATA TATCCATGCT
    AATTACCAGC CAGTGGAAAT TCCAGTTTCA CATAAGCCTG AAAGTAAAGC AGCTCTTTTA
    AAAAAGAAGA ACAGCAGCTT CTCTAAGAAA GACTTTGCTC CTAGTGAAAA GCATGAGCAA
    GCTGATGAAG ATCTGCTCTC TCAATATGAA AATGGTAGCT CCACAGTAGT TGAGGCTAAG
    ACGTCTGAAG CCAGGCCCTT TAATGATTCT ACTCATGCTG AGCCCTTGAA TGATTCTACT
    CACATTTCTT TGCAAGAAGA AAACCAGTCT TCTGTCAGTC ATTGTGTCCC TGATGTTTCT
    ACAATTACTG AAGAAGGCTT ATTTAGCCAA AAGAGTTTCC TTGTTTTGGG TTTTAGTAAT
    GAAAATGAAT CTAACATCGC AAACATCATA AAAGAAAATG CTGGGAAAAT CATGTCCCTT
    CTGAGCAGAA CTGTTGCGGA TTATGCTGTG GTTCCTCTGC TGGGGTGTGA AGTGGAAGCC
    ACTGTGGGAG AAGTTGTTAC AAATACATGG CTGGTTACTT GCATAGACTA TCAGACTTTG
    TTTGATCCAA AGTCGAATCC TCTCTTCACA CCAGTTCCAG TAATGACAGG AATGACTCCT
    TTAGAGGATT GTGTTATTTC ATTTAGCCAG TGTGCTGGAG CAGAAAAAGA GTCTTTAACA
    TTCCTAGCAA ACCTCCTTGG AGCAAGTGTT CAAGAATACT TTGTTCGCAA ATCCAATGCA
    AAGAAAGGCA TGTTTGCCAG TACTCATCTT ATACTGAAAG AACGTGGTGG CTCTAAATAT
    GAAGCTGCAA AGAAGTGGAA TTTACCTGCC GTTACTATAG CTTGGCTGTT GGAGACTGCT
    AGAACGGGAA AGAGAGCAGA CGAAAGCCAT TTTCTGATTG AAAATTCAAC TAAAGAAGAA
    CGAAGTTTGG AAACAGAAAT AACAAATGGA ATCAATCTAA ATTCAGATAC TGCAGAGCAT
    CCTGGCACAC GCCTGCAAAC TCACAGAAAA ACCGTCGTTA CACCTTTAGA TATGAACCGC
    TTTCAGAGTA AAGCTTTCCG TGCTGTGGTC TCACAACATG CCAGACAGGT CGCAGCCTCC
    CCAGCAGTAG GACAACCACT TCAGAAGGAG CCCTCGTTAC ACCTGGATAC ACCATCAAAA
    TTCCTGTCCA AGGACAAACT CTTCAAGCCT TCCTTTGATG TGAAGGATGC ACTTGCAGCC
    TTGGAAACTC CAGGACGTCC CAGCCAACAG AAAAGGAAAC CGAGTACGCC ACTCTCAGAA
    GTTATTGTCA AAAACTTGCA ACTTGCTTTG GCAAATAGCT CTCGAAATGC TGTCGCTCTT
    TCTGCCAGCC CTCAACTGAA AGAGGCCCAG TCAGAGAAGG AAGAAGCCCC AAAGCCACTT
    CACAAAGTAG TGGTATGTGT TAGTAAAAAA CTCAGTAAGA AGCAGAGTGA ACTAAATGGG
    ATCGCAGCCT CTCTAGGAGC AGATTACAGG TGGAGTTTTG ATGAAACAGT GACTCATTTC
    ATCTATCAAG GGCGGCCAAA TGACACTAAT CGGGAGTATA AATCTGTAAA AGAAAGAGGA
    GTACACATTG TTTCCGAGCA CTGGCTTTTA GATTGTGCCC AAGAGTGTAA ACATCTTCCT
    GAATCTCTTT ATCCACATAC TTATAATCCC AAAATGAGCT TGGATATCAG CGCAGTGCAA
    GATGGCCGGC TCTGTAATAG TCGACTACTC TCAGCTGTGT CTTCAACAAA GGATGATGAG
    CCAGATCCTT TGATTTTAGA AGAAAATGAT GTAGACAATA TGGCCACCAA TAATAAAGAG
    TCAGCACCAT CAAATGGAAG TGGAAAGAAT GACTCTAAAG GAGTTCTGAC ACAGACCTTA
    GAGATGAGAG AGAACTTTCA GAAGCAGTTA CAGGAGATAA TGTCTGCAAC ATCAATAGTG
    AAACCCCAAG GGCAGAGGAC TTCCCTTTCA AGAAGTGGTT GTAACAGCGC ATCTTCAACC
    CCTGACAGCA CTCGCTCTGC TCGCAGTGGA CGAAGTAGAG TCCTAGAGGC ACTGAGGCAG
    TCTCGTCAGA CAGTACCTGA TGTCAACACA GAGCCTTCCC AAAATGAACA GATCATTTGG
    GATGACCCTA CAGCAAGGGA GGAGAGAGCA AGGCTTGCCA GCAATTTGCA GTGGCCTAGT
    TGTCCCACAC AATACTCTGA GCTTCAGGTT GACATTCAAA ACTTGGAGGA TTCTCCTTTT
    CAAAAGCCTT TACATGATTC AGAAATTGCT AAACAGGCTG TCTGTGATCC TGGAAACATA
    CGTGTGACTG AAGCTCCCAA ACACCCAATC TCTGAAGAAC TGGAAACTCC CATAAAAGAC
    AGCCACCTGA TCCCTACGCC TCAAGCCCCC AGTATTGCCT TTCCACTCGC CAACCCCCCT
    GTGGCTCCGC ACCCTAGAGA AAAGATTATA ACGATAGAGG AGACTCATGA AGAATTAAAA
    AAACAGTACA TATTTCAGTT ATCATCTCTG AATCCTCAAG AACGTATTGA CTATTGTCAT
    CTGATTGAGA AACTAGGTGG ATTGGTGATA GAAAAGCAGT GCTTTGATCC CACCTGTACA
    CACATTGTTG TGGGACATCC ACTTCGAAAC GAGAAGTATT TAGCCTCAGT GGCAGCTGGG
    AAGTGGGTGC TTCATCGCTC CTACCTTGAA GCCTGCAGGA CTGCTGGACA CTTCGTGCAG
    GAAGAAGACT ATGAATGGGG AAGTAGTTCC ATACTTGATG TTCTGACTGG AATCAATGTA
    CAGCAACGAA GACTAGCACT TGCAGCAATG AGATGGAGAA AAAAAATCCA GCAAAGACAA
    GAATCTGGCA TTGTTGAGGG AGCATTTAGT GGGTGGAAGG TTATTTTACA TGTGGATCAG
    TCTCGAGAAG CAGGCTTCAA ACGCCTTCTT CAGTCAGGAG GAGCAAAGGT GCTACCTGGT
    CATTCTGTAC CTTTATTTAA AGAGGCCACA CATCTTTTTT CTGACTTGAA TAAACTGAAA
    CCAGATGACT CAGGAGTTAA TATAGCAGAA GCTGCTGCCC AGAACGTGTA CTGCTTGAGA
    ACAGAATACA TTGCTGATTA TCTCATGCAG GAATCACCTC CTCATGTAGA AAATTACTGT
    CTACCAGAAG CTATTTCATT TATTCAGAAT AATAAGGAAC TTGGGACTGG ATTATCACAA
    AAGAGGAAAG CTCCTACAGA AAAAAATAAA ATCAAACGAC CTAGAGTACA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001350818.1
    CDS181..4734
    Translation

    Target ORF information:

    RefSeq Version NM_001363889.2
    Organism Homo sapiens(human)
    Definition Homo sapiens DNA topoisomerase II binding protein 1 (TOPBP1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001363889.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTCCAGAA ATGACAAAGA ACCGTTTTTT GTGAAGTTTT TAAAGTCTTC AGACAATTCC 
    AAATGTTTTT TTAAAGCTCT CGAGTCCATA AAAGAATTCC AATCAGAAGA ATATCTTCAG
    ATTATTACAG AAGAAGAGGC ATTGAAGATA AAGGAGAATG ATAGATCACT TTATATCTGT
    GACCCTTTTA GTGGCGTTGT CTTTGATCAC CTCAAAAAGC TTGGCTGCAG AATTGTTGGT
    CCTCAAGTAG TCATATTTTG TATGCACCAC CAGCGATGTG TCCCAAGAGC CGAACATCCA
    GTTTATAATA TGGTTATGTC TGATGTAACC ATATCTTGTA CAAGTCTGGA AAAAGAAAAA
    AGGGAAGAAG TTCATAAATA TGTACAAATG ATGGGCGGAC GAGTATACAG AGACCTTAAT
    GTATCAGTAA CTCACCTTAT TGCAGGAGAA GTTGGTAGCA AAAAATATTT AGTTGCTGCA
    AACCTGAAGA AACCTATTTT GCTTCCCTCT TGGATAAAAA CACTTTGGGA GAAGTCACAA
    GAGAAAAAAA TAACTAGATA TACTGATATA AACATGGAAG ATTTCAAGTG TCCTATTTTT
    CTTGGTTGCA TAATCTGTGT GACTGGCTTA TGTGGCTTAG ACAGGAAAGA AGTTCAGCAA
    CTCACAGTTA AGCATGGAGG TCAATACATG GGACAATTGA AAATGAATGA ATGTACACAC
    CTCATTGTGC AAGAACCAAA AGGTCAGAAG TATGAGTGTG CCAAGAGATG GAATGTACAC
    TGTGTGACCA CACAGTGGTT TTTTGACAGT ATTGAGAAAG GTTTTTGTCA GGATGAATCC
    ATATACAAGA CAGAACCTAG ACCAGAAGCA AAGACTATGC CCAATTCTTC AACTCCTACC
    AGCCAGATCA ACACAATTGA TAATGTCAGC AATATTTCCA ACATAAATGC AAGTTGCGTA
    AGTGAATCAA TATGTAATTC ACTTAACAGC AAACTGGAGC CTACACTTGA AAATCTAGAA
    AATCTGGATG TCAGTGCATT TCAAGCACCT GAAGATTTAT TAGATGGTTG TCGGATATAT
    CTTTGCGGTT TTAGTGGCAG AAAGCTAGAT AAACTGAGAA GACTTATTAA CAGTGGAGGT
    GGAGTTCGTT TTAACCAGCT AAATGAAGAT GTAACTCATG TTATTGTGGG AGATTATGAT
    GATGAATTGA AGCAGTTTTG GAATAAATCA GCCCACAGGC CTCATGTAGT GGGAGCAAAG
    TGGTTGCTAG AGTGTTTCAG TAAAGGTTAT ATGCTTTCTG AAGAACCATA TATCCATGCT
    AATTACCAGC CAGTGGAAAT TCCAGTTTCA CATAAGCCTG AAAGTAAAGC AGCTCTTTTA
    AAAAAGAAGA ACAGCAGCTT CTCTAAGAAA GACTTTGCTC CTAGTGAAAA GCATGAGCAA
    GCTGATGAAG ATCTGCTCTC TCAATATGAA AATGGTAGCT CCACAGTAGT TGAGGCTAAG
    ACGTCTGAAG CCAGGCCCTT TAATGATTCT ACTCATGCTG AGCCCTTGAA TGATTCTACT
    CACATTTCTT TGCAAGAAGA AAACCAGTCT TCTGTCAGTC ATTGTGTCCC TGATGTTTCT
    ACAATTACTG AAGAAGGCTT ATTTAGCCAA AAGAGTTTCC TTGTTTTGGG TTTTAGTAAT
    GAAAATGAAT CTAACATCGC AAACATCATA AAAGAAAATG CTGGGAAAAT CATGTCCCTT
    CTGAGCAGAA CTGTTGCGGA TTATGCTGTG GTTCCTCTGC TGGGGTGTGA AGTGGAAGCC
    ACTGTGGGAG AAGTTGTTAC AAATACATGG CTGGTTACTT GCATAGACTA TCAGACTTTG
    TTTGATCCAA AGTCGAATCC TCTCTTCACA CCAGTTCCAG TAATGACAGG AATGACTCCT
    TTAGAGGATT GTGTTATTTC ATTTAGCCAG TGTGCTGGAG CAGAAAAAGA GTCTTTAACA
    TTCCTAGCAA ACCTCCTTGG AGCAAGTGTT CAAGAATACT TTGTTCGCAA ATCCAATGCA
    AAGAAAGGCA TGTTTGCCAG TACTCATCTT ATACTGAAAG AACGTGGTGG CTCTAAATAT
    GAAGCTGCAA AGAAGTGGAA TTTACCTGCC GTTACTATAG CTTGGCTGTT GGAGACTGCT
    AGAACGGGAA AGAGAGCAGA CGAAAGCCAT TTTCTGATTG AAAATTCAAC TAAAGAAGAA
    CGAAGTTTGG AAACAGAAAT AACAAATGGA ATCAATCTAA ATTCAGATAC TGCAGAGCAT
    CCTGGCACAC GCCTGCAAAC TCACAGAAAA ACCGTCGTTA CACCTTTAGA TATGAACCGC
    TTTCAGAGTA AAGCTTTCCG TGCTGTGGTC TCACAACATG CCAGACAGGT CGCAGCCTCC
    CCAGCAGTAG GACAACCACT TCAGAAGGAG CCCTCGTTAC ACCTGGATAC ACCATCAAAA
    TTCCTGTCCA AGGACAAACT CTTCAAGCCT TCCTTTGATG TGAAGGATGC ACTTGCAGCC
    TTGGAAACTC CAGGACGTCC CAGCCAACAG AAAAGGAAAC CGAGTACGCC ACTCTCAGAA
    GTTATTGTCA AAAACTTGCA ACTTGCTTTG GCAAATAGCT CTCGAAATGC TGTCGCTCTT
    TCTGCCAGCC CTCAACTGAA AGAGGCCCAG TCAGAGAAGG AAGAAGCCCC AAAGCCACTT
    CACAAAGTAG TGGTATGTGT TAGTAAAAAA CTCAGTAAGA AGCAGAGTGA ACTAAATGGG
    ATCGCAGCCT CTCTAGGAGC AGATTACAGG TGGAGTTTTG ATGAAACAGT GACTCATTTC
    ATCTATCAAG GGCGGCCAAA TGACACTAAT CGGGAGTATA AATCTGTAAA AGAAAGAGGA
    GTACACATTG TTTCCGAGCA CTGGCTTTTA GATTGTGCCC AAGAGTGTAA ACATCTTCCT
    GAATCTCTTT ATCCACATAC TTATAATCCC AAAATGAGCT TGGATATCAG CGCAGTGCAA
    GATGGCCGGC TCTGTAATAG TCGACTACTC TCAGCTGTGT CTTCAACAAA GGATGATGAG
    CCAGATCCTT TGATTTTAGA AGAAAATGAT GTAGACAATA TGGCCACCAA TAATAAAGAG
    TCAGCACCAT CAAATGGAAG TGGAAAGAAT GACTCTAAAG GAGTTCTGAC ACAGACCTTA
    GAGATGAGAG AGAACTTTCA GAAGCAGTTA CAGGAGATAA TGTCTGCAAC ATCAATAGTG
    AAACCCCAAG GGCAGAGGAC TTCCCTTTCA AGAAGTGGTT GTAACAGCGC ATCTTCAACC
    CCTGACAGCA CTCGCTCTGC TCGCAGTGGA CGAAGTAGAG TCCTAGAGGC ACTGAGGCAG
    TCTCGTCAGA CAGTACCTGA TGTCAACACA GAGCCTTCCC AAAATGAACA GATCATTTGG
    GATGACCCTA CAGCAAGGGA GGAGAGAGCA AGGCTTGCCA GCAATTTGCA GTGGCCTAGT
    TGTCCCACAC AATACTCTGA GCTTCAGGTT GACATTCAAA ACTTGGAGGA TTCTCCTTTT
    CAAAAGCCTT TACATGATTC AGAAATTGCT AAACAGGCTG TCTGTGATCC TGGAAACATA
    CGTGTGACTG AAGCTCCCAA ACACCCAATC TCTGAAGAAC TGGAAACTCC CATAAAAGAC
    AGCCACCTGA TCCCTACGCC TCAAGCCCCC AGTATTGCCT TTCCACTCGC CAACCCCCCT
    GTGGCTCCGC ACCCTAGAGA AAAGATTATA ACGATAGAGG AGACTCATGA AGAATTAAAA
    AAACAGTACA TATTTCAGTT ATCATCTCTG AATCCTCAAG AACGTATTGA CTATTGTCAT
    CTGATTGAGA AACTAGGTGG ATTGGTGATA GAAAAGCAGT GCTTTGATCC CACCTGTACA
    CACATTGTTG TGGGACATCC ACTTCGAAAC GAGAAGTATT TAGCCTCAGT GGCAGCTGGG
    AAGTGGGTGC TTCATCGCTC CTACCTTGAA GCCTGCAGGA CTGCTGGACA CTTCGTGCAG
    GAAGAAGACT ATGAATGGGG AAGTAGTTCC ATACTTGATG TTCTGACTGG AATCAATGTA
    CAGCAACGAA GACTAGCACT TGCAGCAATG AGATGGAGAA AAAAAATCCA GCAAAGACAA
    GAATCTGGCA TTGTTGAGGG AGCATTTAGT GGGTGGAAGG TTATTTTACA TGTGGATCAG
    TCTCGAGAAG CAGGCTTCAA ACGCCTTCTT CAGTCAGGAG GAGCAAAGGT GCTACCTGGT
    CATTCTGTAC CTTTATTTAA AGAGGCCACA CATCTTTTTT CTGACTTGAA TAAACTGAAA
    CCAGATGACT CAGGAGTTAA TATAGCAGAA GCTGCTGCCC AGAACGTGTA CTGCTTGAGA
    ACAGAATACA TTGCTGATTA TCTCATGCAG GAATCACCTC CTCATGTAGA AAATTACTGT
    CTACCAGAAG CTATTTCATT TATTCAGAAT AATAAGGAAC TTGGGACTGG ATTATCACAA
    AAGAGGAAAG CTCCTACAGA AAAAAATAAA ATCAAACGAC CTAGAGTACA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu37681
    Clone ID Related Accession (Same CDS sequence) NM_001363889.2 , XM_005247076.3 , NM_001363889.1
    Accession Version NM_001363889.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4554bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-27
    Organism Homo sapiens(human)
    Product DNA topoisomerase 2-binding protein 1 isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC083905.20 and AC016255.24. On May 31, 2018 this sequence version replaced XM_005247076.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.22551.1, SRR1660809.149740.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTCCAGAA ATGACAAAGA ACCGTTTTTT GTGAAGTTTT TAAAGTCTTC AGACAATTCC 
    AAATGTTTTT TTAAAGCTCT CGAGTCCATA AAAGAATTCC AATCAGAAGA ATATCTTCAG
    ATTATTACAG AAGAAGAGGC ATTGAAGATA AAGGAGAATG ATAGATCACT TTATATCTGT
    GACCCTTTTA GTGGCGTTGT CTTTGATCAC CTCAAAAAGC TTGGCTGCAG AATTGTTGGT
    CCTCAAGTAG TCATATTTTG TATGCACCAC CAGCGATGTG TCCCAAGAGC CGAACATCCA
    GTTTATAATA TGGTTATGTC TGATGTAACC ATATCTTGTA CAAGTCTGGA AAAAGAAAAA
    AGGGAAGAAG TTCATAAATA TGTACAAATG ATGGGCGGAC GAGTATACAG AGACCTTAAT
    GTATCAGTAA CTCACCTTAT TGCAGGAGAA GTTGGTAGCA AAAAATATTT AGTTGCTGCA
    AACCTGAAGA AACCTATTTT GCTTCCCTCT TGGATAAAAA CACTTTGGGA GAAGTCACAA
    GAGAAAAAAA TAACTAGATA TACTGATATA AACATGGAAG ATTTCAAGTG TCCTATTTTT
    CTTGGTTGCA TAATCTGTGT GACTGGCTTA TGTGGCTTAG ACAGGAAAGA AGTTCAGCAA
    CTCACAGTTA AGCATGGAGG TCAATACATG GGACAATTGA AAATGAATGA ATGTACACAC
    CTCATTGTGC AAGAACCAAA AGGTCAGAAG TATGAGTGTG CCAAGAGATG GAATGTACAC
    TGTGTGACCA CACAGTGGTT TTTTGACAGT ATTGAGAAAG GTTTTTGTCA GGATGAATCC
    ATATACAAGA CAGAACCTAG ACCAGAAGCA AAGACTATGC CCAATTCTTC AACTCCTACC
    AGCCAGATCA ACACAATTGA TAATGTCAGC AATATTTCCA ACATAAATGC AAGTTGCGTA
    AGTGAATCAA TATGTAATTC ACTTAACAGC AAACTGGAGC CTACACTTGA AAATCTAGAA
    AATCTGGATG TCAGTGCATT TCAAGCACCT GAAGATTTAT TAGATGGTTG TCGGATATAT
    CTTTGCGGTT TTAGTGGCAG AAAGCTAGAT AAACTGAGAA GACTTATTAA CAGTGGAGGT
    GGAGTTCGTT TTAACCAGCT AAATGAAGAT GTAACTCATG TTATTGTGGG AGATTATGAT
    GATGAATTGA AGCAGTTTTG GAATAAATCA GCCCACAGGC CTCATGTAGT GGGAGCAAAG
    TGGTTGCTAG AGTGTTTCAG TAAAGGTTAT ATGCTTTCTG AAGAACCATA TATCCATGCT
    AATTACCAGC CAGTGGAAAT TCCAGTTTCA CATAAGCCTG AAAGTAAAGC AGCTCTTTTA
    AAAAAGAAGA ACAGCAGCTT CTCTAAGAAA GACTTTGCTC CTAGTGAAAA GCATGAGCAA
    GCTGATGAAG ATCTGCTCTC TCAATATGAA AATGGTAGCT CCACAGTAGT TGAGGCTAAG
    ACGTCTGAAG CCAGGCCCTT TAATGATTCT ACTCATGCTG AGCCCTTGAA TGATTCTACT
    CACATTTCTT TGCAAGAAGA AAACCAGTCT TCTGTCAGTC ATTGTGTCCC TGATGTTTCT
    ACAATTACTG AAGAAGGCTT ATTTAGCCAA AAGAGTTTCC TTGTTTTGGG TTTTAGTAAT
    GAAAATGAAT CTAACATCGC AAACATCATA AAAGAAAATG CTGGGAAAAT CATGTCCCTT
    CTGAGCAGAA CTGTTGCGGA TTATGCTGTG GTTCCTCTGC TGGGGTGTGA AGTGGAAGCC
    ACTGTGGGAG AAGTTGTTAC AAATACATGG CTGGTTACTT GCATAGACTA TCAGACTTTG
    TTTGATCCAA AGTCGAATCC TCTCTTCACA CCAGTTCCAG TAATGACAGG AATGACTCCT
    TTAGAGGATT GTGTTATTTC ATTTAGCCAG TGTGCTGGAG CAGAAAAAGA GTCTTTAACA
    TTCCTAGCAA ACCTCCTTGG AGCAAGTGTT CAAGAATACT TTGTTCGCAA ATCCAATGCA
    AAGAAAGGCA TGTTTGCCAG TACTCATCTT ATACTGAAAG AACGTGGTGG CTCTAAATAT
    GAAGCTGCAA AGAAGTGGAA TTTACCTGCC GTTACTATAG CTTGGCTGTT GGAGACTGCT
    AGAACGGGAA AGAGAGCAGA CGAAAGCCAT TTTCTGATTG AAAATTCAAC TAAAGAAGAA
    CGAAGTTTGG AAACAGAAAT AACAAATGGA ATCAATCTAA ATTCAGATAC TGCAGAGCAT
    CCTGGCACAC GCCTGCAAAC TCACAGAAAA ACCGTCGTTA CACCTTTAGA TATGAACCGC
    TTTCAGAGTA AAGCTTTCCG TGCTGTGGTC TCACAACATG CCAGACAGGT CGCAGCCTCC
    CCAGCAGTAG GACAACCACT TCAGAAGGAG CCCTCGTTAC ACCTGGATAC ACCATCAAAA
    TTCCTGTCCA AGGACAAACT CTTCAAGCCT TCCTTTGATG TGAAGGATGC ACTTGCAGCC
    TTGGAAACTC CAGGACGTCC CAGCCAACAG AAAAGGAAAC CGAGTACGCC ACTCTCAGAA
    GTTATTGTCA AAAACTTGCA ACTTGCTTTG GCAAATAGCT CTCGAAATGC TGTCGCTCTT
    TCTGCCAGCC CTCAACTGAA AGAGGCCCAG TCAGAGAAGG AAGAAGCCCC AAAGCCACTT
    CACAAAGTAG TGGTATGTGT TAGTAAAAAA CTCAGTAAGA AGCAGAGTGA ACTAAATGGG
    ATCGCAGCCT CTCTAGGAGC AGATTACAGG TGGAGTTTTG ATGAAACAGT GACTCATTTC
    ATCTATCAAG GGCGGCCAAA TGACACTAAT CGGGAGTATA AATCTGTAAA AGAAAGAGGA
    GTACACATTG TTTCCGAGCA CTGGCTTTTA GATTGTGCCC AAGAGTGTAA ACATCTTCCT
    GAATCTCTTT ATCCACATAC TTATAATCCC AAAATGAGCT TGGATATCAG CGCAGTGCAA
    GATGGCCGGC TCTGTAATAG TCGACTACTC TCAGCTGTGT CTTCAACAAA GGATGATGAG
    CCAGATCCTT TGATTTTAGA AGAAAATGAT GTAGACAATA TGGCCACCAA TAATAAAGAG
    TCAGCACCAT CAAATGGAAG TGGAAAGAAT GACTCTAAAG GAGTTCTGAC ACAGACCTTA
    GAGATGAGAG AGAACTTTCA GAAGCAGTTA CAGGAGATAA TGTCTGCAAC ATCAATAGTG
    AAACCCCAAG GGCAGAGGAC TTCCCTTTCA AGAAGTGGTT GTAACAGCGC ATCTTCAACC
    CCTGACAGCA CTCGCTCTGC TCGCAGTGGA CGAAGTAGAG TCCTAGAGGC ACTGAGGCAG
    TCTCGTCAGA CAGTACCTGA TGTCAACACA GAGCCTTCCC AAAATGAACA GATCATTTGG
    GATGACCCTA CAGCAAGGGA GGAGAGAGCA AGGCTTGCCA GCAATTTGCA GTGGCCTAGT
    TGTCCCACAC AATACTCTGA GCTTCAGGTT GACATTCAAA ACTTGGAGGA TTCTCCTTTT
    CAAAAGCCTT TACATGATTC AGAAATTGCT AAACAGGCTG TCTGTGATCC TGGAAACATA
    CGTGTGACTG AAGCTCCCAA ACACCCAATC TCTGAAGAAC TGGAAACTCC CATAAAAGAC
    AGCCACCTGA TCCCTACGCC TCAAGCCCCC AGTATTGCCT TTCCACTCGC CAACCCCCCT
    GTGGCTCCGC ACCCTAGAGA AAAGATTATA ACGATAGAGG AGACTCATGA AGAATTAAAA
    AAACAGTACA TATTTCAGTT ATCATCTCTG AATCCTCAAG AACGTATTGA CTATTGTCAT
    CTGATTGAGA AACTAGGTGG ATTGGTGATA GAAAAGCAGT GCTTTGATCC CACCTGTACA
    CACATTGTTG TGGGACATCC ACTTCGAAAC GAGAAGTATT TAGCCTCAGT GGCAGCTGGG
    AAGTGGGTGC TTCATCGCTC CTACCTTGAA GCCTGCAGGA CTGCTGGACA CTTCGTGCAG
    GAAGAAGACT ATGAATGGGG AAGTAGTTCC ATACTTGATG TTCTGACTGG AATCAATGTA
    CAGCAACGAA GACTAGCACT TGCAGCAATG AGATGGAGAA AAAAAATCCA GCAAAGACAA
    GAATCTGGCA TTGTTGAGGG AGCATTTAGT GGGTGGAAGG TTATTTTACA TGTGGATCAG
    TCTCGAGAAG CAGGCTTCAA ACGCCTTCTT CAGTCAGGAG GAGCAAAGGT GCTACCTGGT
    CATTCTGTAC CTTTATTTAA AGAGGCCACA CATCTTTTTT CTGACTTGAA TAAACTGAAA
    CCAGATGACT CAGGAGTTAA TATAGCAGAA GCTGCTGCCC AGAACGTGTA CTGCTTGAGA
    ACAGAATACA TTGCTGATTA TCTCATGCAG GAATCACCTC CTCATGTAGA AAATTACTGT
    CTACCAGAAG CTATTTCATT TATTCAGAAT AATAAGGAAC TTGGGACTGG ATTATCACAA
    AAGAGGAAAG CTCCTACAGA AAAAAATAAA ATCAAACGAC CTAGAGTACA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001350818.1
    CDS161..4714
    Translation

    Target ORF information:

    RefSeq Version NM_001363889.1
    Organism Homo sapiens(human)
    Definition Homo sapiens DNA topoisomerase II binding protein 1 (TOPBP1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001363889.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTCCAGAA ATGACAAAGA ACCGTTTTTT GTGAAGTTTT TAAAGTCTTC AGACAATTCC 
    AAATGTTTTT TTAAAGCTCT CGAGTCCATA AAAGAATTCC AATCAGAAGA ATATCTTCAG
    ATTATTACAG AAGAAGAGGC ATTGAAGATA AAGGAGAATG ATAGATCACT TTATATCTGT
    GACCCTTTTA GTGGCGTTGT CTTTGATCAC CTCAAAAAGC TTGGCTGCAG AATTGTTGGT
    CCTCAAGTAG TCATATTTTG TATGCACCAC CAGCGATGTG TCCCAAGAGC CGAACATCCA
    GTTTATAATA TGGTTATGTC TGATGTAACC ATATCTTGTA CAAGTCTGGA AAAAGAAAAA
    AGGGAAGAAG TTCATAAATA TGTACAAATG ATGGGCGGAC GAGTATACAG AGACCTTAAT
    GTATCAGTAA CTCACCTTAT TGCAGGAGAA GTTGGTAGCA AAAAATATTT AGTTGCTGCA
    AACCTGAAGA AACCTATTTT GCTTCCCTCT TGGATAAAAA CACTTTGGGA GAAGTCACAA
    GAGAAAAAAA TAACTAGATA TACTGATATA AACATGGAAG ATTTCAAGTG TCCTATTTTT
    CTTGGTTGCA TAATCTGTGT GACTGGCTTA TGTGGCTTAG ACAGGAAAGA AGTTCAGCAA
    CTCACAGTTA AGCATGGAGG TCAATACATG GGACAATTGA AAATGAATGA ATGTACACAC
    CTCATTGTGC AAGAACCAAA AGGTCAGAAG TATGAGTGTG CCAAGAGATG GAATGTACAC
    TGTGTGACCA CACAGTGGTT TTTTGACAGT ATTGAGAAAG GTTTTTGTCA GGATGAATCC
    ATATACAAGA CAGAACCTAG ACCAGAAGCA AAGACTATGC CCAATTCTTC AACTCCTACC
    AGCCAGATCA ACACAATTGA TAATGTCAGC AATATTTCCA ACATAAATGC AAGTTGCGTA
    AGTGAATCAA TATGTAATTC ACTTAACAGC AAACTGGAGC CTACACTTGA AAATCTAGAA
    AATCTGGATG TCAGTGCATT TCAAGCACCT GAAGATTTAT TAGATGGTTG TCGGATATAT
    CTTTGCGGTT TTAGTGGCAG AAAGCTAGAT AAACTGAGAA GACTTATTAA CAGTGGAGGT
    GGAGTTCGTT TTAACCAGCT AAATGAAGAT GTAACTCATG TTATTGTGGG AGATTATGAT
    GATGAATTGA AGCAGTTTTG GAATAAATCA GCCCACAGGC CTCATGTAGT GGGAGCAAAG
    TGGTTGCTAG AGTGTTTCAG TAAAGGTTAT ATGCTTTCTG AAGAACCATA TATCCATGCT
    AATTACCAGC CAGTGGAAAT TCCAGTTTCA CATAAGCCTG AAAGTAAAGC AGCTCTTTTA
    AAAAAGAAGA ACAGCAGCTT CTCTAAGAAA GACTTTGCTC CTAGTGAAAA GCATGAGCAA
    GCTGATGAAG ATCTGCTCTC TCAATATGAA AATGGTAGCT CCACAGTAGT TGAGGCTAAG
    ACGTCTGAAG CCAGGCCCTT TAATGATTCT ACTCATGCTG AGCCCTTGAA TGATTCTACT
    CACATTTCTT TGCAAGAAGA AAACCAGTCT TCTGTCAGTC ATTGTGTCCC TGATGTTTCT
    ACAATTACTG AAGAAGGCTT ATTTAGCCAA AAGAGTTTCC TTGTTTTGGG TTTTAGTAAT
    GAAAATGAAT CTAACATCGC AAACATCATA AAAGAAAATG CTGGGAAAAT CATGTCCCTT
    CTGAGCAGAA CTGTTGCGGA TTATGCTGTG GTTCCTCTGC TGGGGTGTGA AGTGGAAGCC
    ACTGTGGGAG AAGTTGTTAC AAATACATGG CTGGTTACTT GCATAGACTA TCAGACTTTG
    TTTGATCCAA AGTCGAATCC TCTCTTCACA CCAGTTCCAG TAATGACAGG AATGACTCCT
    TTAGAGGATT GTGTTATTTC ATTTAGCCAG TGTGCTGGAG CAGAAAAAGA GTCTTTAACA
    TTCCTAGCAA ACCTCCTTGG AGCAAGTGTT CAAGAATACT TTGTTCGCAA ATCCAATGCA
    AAGAAAGGCA TGTTTGCCAG TACTCATCTT ATACTGAAAG AACGTGGTGG CTCTAAATAT
    GAAGCTGCAA AGAAGTGGAA TTTACCTGCC GTTACTATAG CTTGGCTGTT GGAGACTGCT
    AGAACGGGAA AGAGAGCAGA CGAAAGCCAT TTTCTGATTG AAAATTCAAC TAAAGAAGAA
    CGAAGTTTGG AAACAGAAAT AACAAATGGA ATCAATCTAA ATTCAGATAC TGCAGAGCAT
    CCTGGCACAC GCCTGCAAAC TCACAGAAAA ACCGTCGTTA CACCTTTAGA TATGAACCGC
    TTTCAGAGTA AAGCTTTCCG TGCTGTGGTC TCACAACATG CCAGACAGGT CGCAGCCTCC
    CCAGCAGTAG GACAACCACT TCAGAAGGAG CCCTCGTTAC ACCTGGATAC ACCATCAAAA
    TTCCTGTCCA AGGACAAACT CTTCAAGCCT TCCTTTGATG TGAAGGATGC ACTTGCAGCC
    TTGGAAACTC CAGGACGTCC CAGCCAACAG AAAAGGAAAC CGAGTACGCC ACTCTCAGAA
    GTTATTGTCA AAAACTTGCA ACTTGCTTTG GCAAATAGCT CTCGAAATGC TGTCGCTCTT
    TCTGCCAGCC CTCAACTGAA AGAGGCCCAG TCAGAGAAGG AAGAAGCCCC AAAGCCACTT
    CACAAAGTAG TGGTATGTGT TAGTAAAAAA CTCAGTAAGA AGCAGAGTGA ACTAAATGGG
    ATCGCAGCCT CTCTAGGAGC AGATTACAGG TGGAGTTTTG ATGAAACAGT GACTCATTTC
    ATCTATCAAG GGCGGCCAAA TGACACTAAT CGGGAGTATA AATCTGTAAA AGAAAGAGGA
    GTACACATTG TTTCCGAGCA CTGGCTTTTA GATTGTGCCC AAGAGTGTAA ACATCTTCCT
    GAATCTCTTT ATCCACATAC TTATAATCCC AAAATGAGCT TGGATATCAG CGCAGTGCAA
    GATGGCCGGC TCTGTAATAG TCGACTACTC TCAGCTGTGT CTTCAACAAA GGATGATGAG
    CCAGATCCTT TGATTTTAGA AGAAAATGAT GTAGACAATA TGGCCACCAA TAATAAAGAG
    TCAGCACCAT CAAATGGAAG TGGAAAGAAT GACTCTAAAG GAGTTCTGAC ACAGACCTTA
    GAGATGAGAG AGAACTTTCA GAAGCAGTTA CAGGAGATAA TGTCTGCAAC ATCAATAGTG
    AAACCCCAAG GGCAGAGGAC TTCCCTTTCA AGAAGTGGTT GTAACAGCGC ATCTTCAACC
    CCTGACAGCA CTCGCTCTGC TCGCAGTGGA CGAAGTAGAG TCCTAGAGGC ACTGAGGCAG
    TCTCGTCAGA CAGTACCTGA TGTCAACACA GAGCCTTCCC AAAATGAACA GATCATTTGG
    GATGACCCTA CAGCAAGGGA GGAGAGAGCA AGGCTTGCCA GCAATTTGCA GTGGCCTAGT
    TGTCCCACAC AATACTCTGA GCTTCAGGTT GACATTCAAA ACTTGGAGGA TTCTCCTTTT
    CAAAAGCCTT TACATGATTC AGAAATTGCT AAACAGGCTG TCTGTGATCC TGGAAACATA
    CGTGTGACTG AAGCTCCCAA ACACCCAATC TCTGAAGAAC TGGAAACTCC CATAAAAGAC
    AGCCACCTGA TCCCTACGCC TCAAGCCCCC AGTATTGCCT TTCCACTCGC CAACCCCCCT
    GTGGCTCCGC ACCCTAGAGA AAAGATTATA ACGATAGAGG AGACTCATGA AGAATTAAAA
    AAACAGTACA TATTTCAGTT ATCATCTCTG AATCCTCAAG AACGTATTGA CTATTGTCAT
    CTGATTGAGA AACTAGGTGG ATTGGTGATA GAAAAGCAGT GCTTTGATCC CACCTGTACA
    CACATTGTTG TGGGACATCC ACTTCGAAAC GAGAAGTATT TAGCCTCAGT GGCAGCTGGG
    AAGTGGGTGC TTCATCGCTC CTACCTTGAA GCCTGCAGGA CTGCTGGACA CTTCGTGCAG
    GAAGAAGACT ATGAATGGGG AAGTAGTTCC ATACTTGATG TTCTGACTGG AATCAATGTA
    CAGCAACGAA GACTAGCACT TGCAGCAATG AGATGGAGAA AAAAAATCCA GCAAAGACAA
    GAATCTGGCA TTGTTGAGGG AGCATTTAGT GGGTGGAAGG TTATTTTACA TGTGGATCAG
    TCTCGAGAAG CAGGCTTCAA ACGCCTTCTT CAGTCAGGAG GAGCAAAGGT GCTACCTGGT
    CATTCTGTAC CTTTATTTAA AGAGGCCACA CATCTTTTTT CTGACTTGAA TAAACTGAAA
    CCAGATGACT CAGGAGTTAA TATAGCAGAA GCTGCTGCCC AGAACGTGTA CTGCTTGAGA
    ACAGAATACA TTGCTGATTA TCTCATGCAG GAATCACCTC CTCATGTAGA AAATTACTGT
    CTACCAGAAG CTATTTCATT TATTCAGAAT AATAAGGAAC TTGGGACTGG ATTATCACAA
    AAGAGGAAAG CTCCTACAGA AAAAAATAAA ATCAAACGAC CTAGAGTACA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu37681
    Clone ID Related Accession (Same CDS sequence) NM_001363889.2 , XM_005247076.3 , NM_001363889.1
    Accession Version XM_005247076.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4554bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product DNA topoisomerase 2-binding protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005247076.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTCCAGAA ATGACAAAGA ACCGTTTTTT GTGAAGTTTT TAAAGTCTTC AGACAATTCC 
    AAATGTTTTT TTAAAGCTCT CGAGTCCATA AAAGAATTCC AATCAGAAGA ATATCTTCAG
    ATTATTACAG AAGAAGAGGC ATTGAAGATA AAGGAGAATG ATAGATCACT TTATATCTGT
    GACCCTTTTA GTGGCGTTGT CTTTGATCAC CTCAAAAAGC TTGGCTGCAG AATTGTTGGT
    CCTCAAGTAG TCATATTTTG TATGCACCAC CAGCGATGTG TCCCAAGAGC CGAACATCCA
    GTTTATAATA TGGTTATGTC TGATGTAACC ATATCTTGTA CAAGTCTGGA AAAAGAAAAA
    AGGGAAGAAG TTCATAAATA TGTACAAATG ATGGGCGGAC GAGTATACAG AGACCTTAAT
    GTATCAGTAA CTCACCTTAT TGCAGGAGAA GTTGGTAGCA AAAAATATTT AGTTGCTGCA
    AACCTGAAGA AACCTATTTT GCTTCCCTCT TGGATAAAAA CACTTTGGGA GAAGTCACAA
    GAGAAAAAAA TAACTAGATA TACTGATATA AACATGGAAG ATTTCAAGTG TCCTATTTTT
    CTTGGTTGCA TAATCTGTGT GACTGGCTTA TGTGGCTTAG ACAGGAAAGA AGTTCAGCAA
    CTCACAGTTA AGCATGGAGG TCAATACATG GGACAATTGA AAATGAATGA ATGTACACAC
    CTCATTGTGC AAGAACCAAA AGGTCAGAAG TATGAGTGTG CCAAGAGATG GAATGTACAC
    TGTGTGACCA CACAGTGGTT TTTTGACAGT ATTGAGAAAG GTTTTTGTCA GGATGAATCC
    ATATACAAGA CAGAACCTAG ACCAGAAGCA AAGACTATGC CCAATTCTTC AACTCCTACC
    AGCCAGATCA ACACAATTGA TAATGTCAGC AATATTTCCA ACATAAATGC AAGTTGCGTA
    AGTGAATCAA TATGTAATTC ACTTAACAGC AAACTGGAGC CTACACTTGA AAATCTAGAA
    AATCTGGATG TCAGTGCATT TCAAGCACCT GAAGATTTAT TAGATGGTTG TCGGATATAT
    CTTTGCGGTT TTAGTGGCAG AAAGCTAGAT AAACTGAGAA GACTTATTAA CAGTGGAGGT
    GGAGTTCGTT TTAACCAGCT AAATGAAGAT GTAACTCATG TTATTGTGGG AGATTATGAT
    GATGAATTGA AGCAGTTTTG GAATAAATCA GCCCACAGGC CTCATGTAGT GGGAGCAAAG
    TGGTTGCTAG AGTGTTTCAG TAAAGGTTAT ATGCTTTCTG AAGAACCATA TATCCATGCT
    AATTACCAGC CAGTGGAAAT TCCAGTTTCA CATAAGCCTG AAAGTAAAGC AGCTCTTTTA
    AAAAAGAAGA ACAGCAGCTT CTCTAAGAAA GACTTTGCTC CTAGTGAAAA GCATGAGCAA
    GCTGATGAAG ATCTGCTCTC TCAATATGAA AATGGTAGCT CCACAGTAGT TGAGGCTAAG
    ACGTCTGAAG CCAGGCCCTT TAATGATTCT ACTCATGCTG AGCCCTTGAA TGATTCTACT
    CACATTTCTT TGCAAGAAGA AAACCAGTCT TCTGTCAGTC ATTGTGTCCC TGATGTTTCT
    ACAATTACTG AAGAAGGCTT ATTTAGCCAA AAGAGTTTCC TTGTTTTGGG TTTTAGTAAT
    GAAAATGAAT CTAACATCGC AAACATCATA AAAGAAAATG CTGGGAAAAT CATGTCCCTT
    CTGAGCAGAA CTGTTGCGGA TTATGCTGTG GTTCCTCTGC TGGGGTGTGA AGTGGAAGCC
    ACTGTGGGAG AAGTTGTTAC AAATACATGG CTGGTTACTT GCATAGACTA TCAGACTTTG
    TTTGATCCAA AGTCGAATCC TCTCTTCACA CCAGTTCCAG TAATGACAGG AATGACTCCT
    TTAGAGGATT GTGTTATTTC ATTTAGCCAG TGTGCTGGAG CAGAAAAAGA GTCTTTAACA
    TTCCTAGCAA ACCTCCTTGG AGCAAGTGTT CAAGAATACT TTGTTCGCAA ATCCAATGCA
    AAGAAAGGCA TGTTTGCCAG TACTCATCTT ATACTGAAAG AACGTGGTGG CTCTAAATAT
    GAAGCTGCAA AGAAGTGGAA TTTACCTGCC GTTACTATAG CTTGGCTGTT GGAGACTGCT
    AGAACGGGAA AGAGAGCAGA CGAAAGCCAT TTTCTGATTG AAAATTCAAC TAAAGAAGAA
    CGAAGTTTGG AAACAGAAAT AACAAATGGA ATCAATCTAA ATTCAGATAC TGCAGAGCAT
    CCTGGCACAC GCCTGCAAAC TCACAGAAAA ACCGTCGTTA CACCTTTAGA TATGAACCGC
    TTTCAGAGTA AAGCTTTCCG TGCTGTGGTC TCACAACATG CCAGACAGGT CGCAGCCTCC
    CCAGCAGTAG GACAACCACT TCAGAAGGAG CCCTCGTTAC ACCTGGATAC ACCATCAAAA
    TTCCTGTCCA AGGACAAACT CTTCAAGCCT TCCTTTGATG TGAAGGATGC ACTTGCAGCC
    TTGGAAACTC CAGGACGTCC CAGCCAACAG AAAAGGAAAC CGAGTACGCC ACTCTCAGAA
    GTTATTGTCA AAAACTTGCA ACTTGCTTTG GCAAATAGCT CTCGAAATGC TGTCGCTCTT
    TCTGCCAGCC CTCAACTGAA AGAGGCCCAG TCAGAGAAGG AAGAAGCCCC AAAGCCACTT
    CACAAAGTAG TGGTATGTGT TAGTAAAAAA CTCAGTAAGA AGCAGAGTGA ACTAAATGGG
    ATCGCAGCCT CTCTAGGAGC AGATTACAGG TGGAGTTTTG ATGAAACAGT GACTCATTTC
    ATCTATCAAG GGCGGCCAAA TGACACTAAT CGGGAGTATA AATCTGTAAA AGAAAGAGGA
    GTACACATTG TTTCCGAGCA CTGGCTTTTA GATTGTGCCC AAGAGTGTAA ACATCTTCCT
    GAATCTCTTT ATCCACATAC TTATAATCCC AAAATGAGCT TGGATATCAG CGCAGTGCAA
    GATGGCCGGC TCTGTAATAG TCGACTACTC TCAGCTGTGT CTTCAACAAA GGATGATGAG
    CCAGATCCTT TGATTTTAGA AGAAAATGAT GTAGACAATA TGGCCACCAA TAATAAAGAG
    TCAGCACCAT CAAATGGAAG TGGAAAGAAT GACTCTAAAG GAGTTCTGAC ACAGACCTTA
    GAGATGAGAG AGAACTTTCA GAAGCAGTTA CAGGAGATAA TGTCTGCAAC ATCAATAGTG
    AAACCCCAAG GGCAGAGGAC TTCCCTTTCA AGAAGTGGTT GTAACAGCGC ATCTTCAACC
    CCTGACAGCA CTCGCTCTGC TCGCAGTGGA CGAAGTAGAG TCCTAGAGGC ACTGAGGCAG
    TCTCGTCAGA CAGTACCTGA TGTCAACACA GAGCCTTCCC AAAATGAACA GATCATTTGG
    GATGACCCTA CAGCAAGGGA GGAGAGAGCA AGGCTTGCCA GCAATTTGCA GTGGCCTAGT
    TGTCCCACAC AATACTCTGA GCTTCAGGTT GACATTCAAA ACTTGGAGGA TTCTCCTTTT
    CAAAAGCCTT TACATGATTC AGAAATTGCT AAACAGGCTG TCTGTGATCC TGGAAACATA
    CGTGTGACTG AAGCTCCCAA ACACCCAATC TCTGAAGAAC TGGAAACTCC CATAAAAGAC
    AGCCACCTGA TCCCTACGCC TCAAGCCCCC AGTATTGCCT TTCCACTCGC CAACCCCCCT
    GTGGCTCCGC ACCCTAGAGA AAAGATTATA ACGATAGAGG AGACTCATGA AGAATTAAAA
    AAACAGTACA TATTTCAGTT ATCATCTCTG AATCCTCAAG AACGTATTGA CTATTGTCAT
    CTGATTGAGA AACTAGGTGG ATTGGTGATA GAAAAGCAGT GCTTTGATCC CACCTGTACA
    CACATTGTTG TGGGACATCC ACTTCGAAAC GAGAAGTATT TAGCCTCAGT GGCAGCTGGG
    AAGTGGGTGC TTCATCGCTC CTACCTTGAA GCCTGCAGGA CTGCTGGACA CTTCGTGCAG
    GAAGAAGACT ATGAATGGGG AAGTAGTTCC ATACTTGATG TTCTGACTGG AATCAATGTA
    CAGCAACGAA GACTAGCACT TGCAGCAATG AGATGGAGAA AAAAAATCCA GCAAAGACAA
    GAATCTGGCA TTGTTGAGGG AGCATTTAGT GGGTGGAAGG TTATTTTACA TGTGGATCAG
    TCTCGAGAAG CAGGCTTCAA ACGCCTTCTT CAGTCAGGAG GAGCAAAGGT GCTACCTGGT
    CATTCTGTAC CTTTATTTAA AGAGGCCACA CATCTTTTTT CTGACTTGAA TAAACTGAAA
    CCAGATGACT CAGGAGTTAA TATAGCAGAA GCTGCTGCCC AGAACGTGTA CTGCTTGAGA
    ACAGAATACA TTGCTGATTA TCTCATGCAG GAATCACCTC CTCATGTAGA AAATTACTGT
    CTACCAGAAG CTATTTCATT TATTCAGAAT AATAAGGAAC TTGGGACTGG ATTATCACAA
    AAGAGGAAAG CTCCTACAGA AAAAAATAAA ATCAAACGAC CTAGAGTACA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005247133.1
    CDS620..5173
    Translation

    Target ORF information:

    RefSeq Version XM_005247076.3
    Organism Homo sapiens(human)
    Definition Homo sapiens topoisomerase (DNA) II binding protein 1 (TOPBP1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005247076.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTCCAGAA ATGACAAAGA ACCGTTTTTT GTGAAGTTTT TAAAGTCTTC AGACAATTCC 
    AAATGTTTTT TTAAAGCTCT CGAGTCCATA AAAGAATTCC AATCAGAAGA ATATCTTCAG
    ATTATTACAG AAGAAGAGGC ATTGAAGATA AAGGAGAATG ATAGATCACT TTATATCTGT
    GACCCTTTTA GTGGCGTTGT CTTTGATCAC CTCAAAAAGC TTGGCTGCAG AATTGTTGGT
    CCTCAAGTAG TCATATTTTG TATGCACCAC CAGCGATGTG TCCCAAGAGC CGAACATCCA
    GTTTATAATA TGGTTATGTC TGATGTAACC ATATCTTGTA CAAGTCTGGA AAAAGAAAAA
    AGGGAAGAAG TTCATAAATA TGTACAAATG ATGGGCGGAC GAGTATACAG AGACCTTAAT
    GTATCAGTAA CTCACCTTAT TGCAGGAGAA GTTGGTAGCA AAAAATATTT AGTTGCTGCA
    AACCTGAAGA AACCTATTTT GCTTCCCTCT TGGATAAAAA CACTTTGGGA GAAGTCACAA
    GAGAAAAAAA TAACTAGATA TACTGATATA AACATGGAAG ATTTCAAGTG TCCTATTTTT
    CTTGGTTGCA TAATCTGTGT GACTGGCTTA TGTGGCTTAG ACAGGAAAGA AGTTCAGCAA
    CTCACAGTTA AGCATGGAGG TCAATACATG GGACAATTGA AAATGAATGA ATGTACACAC
    CTCATTGTGC AAGAACCAAA AGGTCAGAAG TATGAGTGTG CCAAGAGATG GAATGTACAC
    TGTGTGACCA CACAGTGGTT TTTTGACAGT ATTGAGAAAG GTTTTTGTCA GGATGAATCC
    ATATACAAGA CAGAACCTAG ACCAGAAGCA AAGACTATGC CCAATTCTTC AACTCCTACC
    AGCCAGATCA ACACAATTGA TAATGTCAGC AATATTTCCA ACATAAATGC AAGTTGCGTA
    AGTGAATCAA TATGTAATTC ACTTAACAGC AAACTGGAGC CTACACTTGA AAATCTAGAA
    AATCTGGATG TCAGTGCATT TCAAGCACCT GAAGATTTAT TAGATGGTTG TCGGATATAT
    CTTTGCGGTT TTAGTGGCAG AAAGCTAGAT AAACTGAGAA GACTTATTAA CAGTGGAGGT
    GGAGTTCGTT TTAACCAGCT AAATGAAGAT GTAACTCATG TTATTGTGGG AGATTATGAT
    GATGAATTGA AGCAGTTTTG GAATAAATCA GCCCACAGGC CTCATGTAGT GGGAGCAAAG
    TGGTTGCTAG AGTGTTTCAG TAAAGGTTAT ATGCTTTCTG AAGAACCATA TATCCATGCT
    AATTACCAGC CAGTGGAAAT TCCAGTTTCA CATAAGCCTG AAAGTAAAGC AGCTCTTTTA
    AAAAAGAAGA ACAGCAGCTT CTCTAAGAAA GACTTTGCTC CTAGTGAAAA GCATGAGCAA
    GCTGATGAAG ATCTGCTCTC TCAATATGAA AATGGTAGCT CCACAGTAGT TGAGGCTAAG
    ACGTCTGAAG CCAGGCCCTT TAATGATTCT ACTCATGCTG AGCCCTTGAA TGATTCTACT
    CACATTTCTT TGCAAGAAGA AAACCAGTCT TCTGTCAGTC ATTGTGTCCC TGATGTTTCT
    ACAATTACTG AAGAAGGCTT ATTTAGCCAA AAGAGTTTCC TTGTTTTGGG TTTTAGTAAT
    GAAAATGAAT CTAACATCGC AAACATCATA AAAGAAAATG CTGGGAAAAT CATGTCCCTT
    CTGAGCAGAA CTGTTGCGGA TTATGCTGTG GTTCCTCTGC TGGGGTGTGA AGTGGAAGCC
    ACTGTGGGAG AAGTTGTTAC AAATACATGG CTGGTTACTT GCATAGACTA TCAGACTTTG
    TTTGATCCAA AGTCGAATCC TCTCTTCACA CCAGTTCCAG TAATGACAGG AATGACTCCT
    TTAGAGGATT GTGTTATTTC ATTTAGCCAG TGTGCTGGAG CAGAAAAAGA GTCTTTAACA
    TTCCTAGCAA ACCTCCTTGG AGCAAGTGTT CAAGAATACT TTGTTCGCAA ATCCAATGCA
    AAGAAAGGCA TGTTTGCCAG TACTCATCTT ATACTGAAAG AACGTGGTGG CTCTAAATAT
    GAAGCTGCAA AGAAGTGGAA TTTACCTGCC GTTACTATAG CTTGGCTGTT GGAGACTGCT
    AGAACGGGAA AGAGAGCAGA CGAAAGCCAT TTTCTGATTG AAAATTCAAC TAAAGAAGAA
    CGAAGTTTGG AAACAGAAAT AACAAATGGA ATCAATCTAA ATTCAGATAC TGCAGAGCAT
    CCTGGCACAC GCCTGCAAAC TCACAGAAAA ACCGTCGTTA CACCTTTAGA TATGAACCGC
    TTTCAGAGTA AAGCTTTCCG TGCTGTGGTC TCACAACATG CCAGACAGGT CGCAGCCTCC
    CCAGCAGTAG GACAACCACT TCAGAAGGAG CCCTCGTTAC ACCTGGATAC ACCATCAAAA
    TTCCTGTCCA AGGACAAACT CTTCAAGCCT TCCTTTGATG TGAAGGATGC ACTTGCAGCC
    TTGGAAACTC CAGGACGTCC CAGCCAACAG AAAAGGAAAC CGAGTACGCC ACTCTCAGAA
    GTTATTGTCA AAAACTTGCA ACTTGCTTTG GCAAATAGCT CTCGAAATGC TGTCGCTCTT
    TCTGCCAGCC CTCAACTGAA AGAGGCCCAG TCAGAGAAGG AAGAAGCCCC AAAGCCACTT
    CACAAAGTAG TGGTATGTGT TAGTAAAAAA CTCAGTAAGA AGCAGAGTGA ACTAAATGGG
    ATCGCAGCCT CTCTAGGAGC AGATTACAGG TGGAGTTTTG ATGAAACAGT GACTCATTTC
    ATCTATCAAG GGCGGCCAAA TGACACTAAT CGGGAGTATA AATCTGTAAA AGAAAGAGGA
    GTACACATTG TTTCCGAGCA CTGGCTTTTA GATTGTGCCC AAGAGTGTAA ACATCTTCCT
    GAATCTCTTT ATCCACATAC TTATAATCCC AAAATGAGCT TGGATATCAG CGCAGTGCAA
    GATGGCCGGC TCTGTAATAG TCGACTACTC TCAGCTGTGT CTTCAACAAA GGATGATGAG
    CCAGATCCTT TGATTTTAGA AGAAAATGAT GTAGACAATA TGGCCACCAA TAATAAAGAG
    TCAGCACCAT CAAATGGAAG TGGAAAGAAT GACTCTAAAG GAGTTCTGAC ACAGACCTTA
    GAGATGAGAG AGAACTTTCA GAAGCAGTTA CAGGAGATAA TGTCTGCAAC ATCAATAGTG
    AAACCCCAAG GGCAGAGGAC TTCCCTTTCA AGAAGTGGTT GTAACAGCGC ATCTTCAACC
    CCTGACAGCA CTCGCTCTGC TCGCAGTGGA CGAAGTAGAG TCCTAGAGGC ACTGAGGCAG
    TCTCGTCAGA CAGTACCTGA TGTCAACACA GAGCCTTCCC AAAATGAACA GATCATTTGG
    GATGACCCTA CAGCAAGGGA GGAGAGAGCA AGGCTTGCCA GCAATTTGCA GTGGCCTAGT
    TGTCCCACAC AATACTCTGA GCTTCAGGTT GACATTCAAA ACTTGGAGGA TTCTCCTTTT
    CAAAAGCCTT TACATGATTC AGAAATTGCT AAACAGGCTG TCTGTGATCC TGGAAACATA
    CGTGTGACTG AAGCTCCCAA ACACCCAATC TCTGAAGAAC TGGAAACTCC CATAAAAGAC
    AGCCACCTGA TCCCTACGCC TCAAGCCCCC AGTATTGCCT TTCCACTCGC CAACCCCCCT
    GTGGCTCCGC ACCCTAGAGA AAAGATTATA ACGATAGAGG AGACTCATGA AGAATTAAAA
    AAACAGTACA TATTTCAGTT ATCATCTCTG AATCCTCAAG AACGTATTGA CTATTGTCAT
    CTGATTGAGA AACTAGGTGG ATTGGTGATA GAAAAGCAGT GCTTTGATCC CACCTGTACA
    CACATTGTTG TGGGACATCC ACTTCGAAAC GAGAAGTATT TAGCCTCAGT GGCAGCTGGG
    AAGTGGGTGC TTCATCGCTC CTACCTTGAA GCCTGCAGGA CTGCTGGACA CTTCGTGCAG
    GAAGAAGACT ATGAATGGGG AAGTAGTTCC ATACTTGATG TTCTGACTGG AATCAATGTA
    CAGCAACGAA GACTAGCACT TGCAGCAATG AGATGGAGAA AAAAAATCCA GCAAAGACAA
    GAATCTGGCA TTGTTGAGGG AGCATTTAGT GGGTGGAAGG TTATTTTACA TGTGGATCAG
    TCTCGAGAAG CAGGCTTCAA ACGCCTTCTT CAGTCAGGAG GAGCAAAGGT GCTACCTGGT
    CATTCTGTAC CTTTATTTAA AGAGGCCACA CATCTTTTTT CTGACTTGAA TAAACTGAAA
    CCAGATGACT CAGGAGTTAA TATAGCAGAA GCTGCTGCCC AGAACGTGTA CTGCTTGAGA
    ACAGAATACA TTGCTGATTA TCTCATGCAG GAATCACCTC CTCATGTAGA AAATTACTGT
    CTACCAGAAG CTATTTCATT TATTCAGAAT AATAAGGAAC TTGGGACTGG ATTATCACAA
    AAGAGGAAAG CTCCTACAGA AAAAAATAAA ATCAAACGAC CTAGAGTACA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.