METAP2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	METAP2
Entrez Gene ID	10988
Full Name	methionyl aminopeptidase 2
Synonyms	MAP2,MNPEP,p67eIF2
General protein information	Preferred Names methionyl aminopeptidase 2 Names methionine aminopeptidase 2 eIF-2-associated p67 homolog initiation factor 2-associated 67 kDa glycoprotein peptidase M 2 testicular tissue protein Li 17
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 12 Map Location: 12q22
Summary	The protein encoded by this gene is a member of the methionyl aminopeptidase family. The encoded protein functions both by protecting the alpha subunit of eukaryotic initiation factor 2 from inhibitory phosphorylation and by removing the amino-terminal methionine residue from nascent proteins. Increased expression of this gene is associated with various forms of cancer, and the anti-cancer drugs fumagillin and ovalicin inhibit the protein by irreversibly binding to its active site. Inhibitors of this gene have also been shown to be effective for the treatment of obesity. A pseudogene of this gene is located on chromosome 2. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2015].
Disorder MIM:	601870

mRNA and Protein(s)

mRNA	Protein	Name
NM_001317183.1	NP_001304112.1	methionine aminopeptidase 2 isoform 3
NM_001317182.1	NP_001304111.1	methionine aminopeptidase 2 isoform 2
NM_001330246.1	NP_001317175.1	methionine aminopeptidase 2 isoform 4
NM_001317183.2	NP_001304112.1	methionine aminopeptidase 2 isoform 3
NM_006838.3	NP_006829.1	methionine aminopeptidase 2 isoform 1
NM_006838.4	NP_006829.1	methionine aminopeptidase 2 isoform 1
NM_001317182.2	NP_001304111.1	methionine aminopeptidase 2 isoform 2
NM_001330246.2	NP_001317175.1	methionine aminopeptidase 2 isoform 4

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Metap2	NP_062622.1
Gallus gallus (chicken)	METAP2	NP_001006233.1
Danio rerio (zebrafish)	metap2a	NP_001093524.1
Drosophila melanogaster (fruit fly)	und	NP_477373.1
Saccharomyces cerevisiae (baker's yeast)	MAP2	NP_009462.2
Eremothecium gossypii	AGOS_AGL283W	NP_986384.2
Schizosaccharomyces pombe (fission yeast)	fma2	NP_595906.1
Oryza sativa (rice)	Os12g0628500	NP_001067330.1
Bos taurus (cattle)	METAP2	NP_001035583.1
Magnaporthe oryzae (rice blast fungus)	MGG_00966	XP_003717984.1
Xenopus tropicalis (tropical clawed frog)	metap2	NP_001016115.1
Homo sapiens (human)	METAP2	NP_006829.1
Neurospora crassa	NCU04306	XP_960952.1
Arabidopsis thaliana (thale cress)	MAP2A	NP_566013.1
Arabidopsis thaliana (thale cress)	MAP2B	NP_001190139.1
Canis lupus familiaris (dog)	METAP2	XP_005629295.1
Pan troglodytes (chimpanzee)	METAP2	XP_003313915.1
	METAP2	XP_001107008.1
Rattus norvegicus (Norway rat)	Metap2	NP_071984.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008345	XP_317113.3
Kluyveromyces lactis	KLLA0E06887g	XP_454259.1

Gene Ontology
Bibliography

Related articles in PubMed

Suppression of glioblastoma growth and angiogenesis through molecular targeting of methionine aminopeptidase-2.
Lin M, Zhang X, Jia B, Guan S
Journal of neuro-oncology136(2)243-254(2018 Jan)

MetAP1 and MetAP2 drive cell selectivity for a potent anti-cancer agent in synergy, by controlling glutathione redox state.
Frottin F, Bienvenut WV, Bignon J, Jacquet E, Vaca Jacome AS, Van Dorsselaer A, Cianferani S, Carapito C, Meinnel T, Giglione C
Oncotarget7(39)63306-63323(2016 Sep)

Methionine Aminopeptidase 2 as a Potential Therapeutic Target for Human Non-Small-Cell Lung Cancers.
Shimizu H, Yamagishi S, Chiba H, Ghazizadeh M
Advances in clinical and experimental medicine : official organ Wroclaw Medical University25(1)117-28(2016 Jan-Feb)

N-terminal acetylome analysis reveals the specificity of Naa50 (Nat5) and suggests a kinetic competition between N-terminal acetyltransferases and methionine aminopeptidases.
Van Damme P, Hole K, Gevaert K, Arnesen T
Proteomics15(14)2436-46(2015 Jul)

Redox regulation of methionine aminopeptidase 2 activity.
Chiu J, Wong JW, Hogg PJ
The Journal of biological chemistry289(21)15035-43(2014 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Suggest MetAP2 as important regulator of proliferation/apoptosis in non-small cell lung cancers.
Title: Methionine Aminopeptidase 2 as a Potential Therapeutic Target for Human Non-Small-Cell Lung Cancers.

Nt-acetylation prevents the excision of the initiator methionine by MetAP2.
Title: N-terminal acetylome analysis reveals the specificity of Naa50 (Nat5) and suggests a kinetic competition between N-terminal acetyltransferases and methionine aminopeptidases.

Data indicate that methionine aminopeptidase 2 (MetAP2) contains a single disulfide bond that exists in oxidized and reduced states and influences enzyme function.
Title: Redox regulation of methionine aminopeptidase 2 activity.

The activity of unmodified, nitrated and oxidised METAP2 was assessed and it was found that nitration significantly reduced its ability to cleave a chromogenic substrate. Mass spectrometry analysis identified Tyr336 as a nitrated residue in METAP2.
Title: Tyrosine nitration moderates the peptidase activity of human methionyl aminopeptidase 2.

Increased expression of METAP2 is associated with pilocytic astrocytoma.
Title: MicroRNA profiling in pediatric pilocytic astrocytoma reveals biologically relevant targets, including PBX3, NFIB, and METAP2.

The following METAP2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the METAP2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu30784	NM_006838.4 Latest version!	Homo sapiens methionyl aminopeptidase 2 (METAP2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu101532	NM_001317183.2 Latest version!	Homo sapiens methionyl aminopeptidase 2 (METAP2), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu101443	NM_001317182.2 Latest version!	Homo sapiens methionyl aminopeptidase 2 (METAP2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$223.30 $319.00
OHu46263	NM_001330246.2 Latest version!	Homo sapiens methionyl aminopeptidase 2 (METAP2), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$223.30 $319.00
View Old Accession Versions >>
OHu101532	NM_001317183.1	Homo sapiens methionyl aminopeptidase 2 (METAP2), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu30784	NM_006838.3	Homo sapiens methionyl aminopeptidase 2 (METAP2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu101443	NM_001317182.1	Homo sapiens methionyl aminopeptidase 2 (METAP2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$223.30 $319.00
OHu46263	NM_001330246.1	Homo sapiens methionyl aminopeptidase 2 (METAP2), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$223.30 $319.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

METAP2 ( NM_006838.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_006829.1 Homo sapiens methionyl aminopeptidase 2 (METAP2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu30784
Clone ID Related Accession (Same CDS sequence)	NM_006838.3 , NM_006838.4
Accession Version	NM_006838.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1437bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	methionine aminopeptidase 2 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DW409106.1, U13261.1 and AC018475.32. On Aug 4, 2006 this sequence version replaced NM_006838.2. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.110914.1, SRR1803616.188010.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGGTG TGGAGGAGGT AGCGGCCTCC GGGAGCCACC TGAATGGCGA CCTGGATCCA 
GACGACAGGG AAGAAGGAGC TGCCTCTACG GCTGAGGAAG CAGCCAAGAA AAAAAGACGA 
AAGAAGAAGA AGAGCAAAGG GCCTTCTGCA GCAGGGGAAC AGGAACCTGA TAAAGAATCA 
GGAGCCTCAG TGGATGAAGT AGCAAGACAG TTGGAAAGAT CAGCATTGGA AGATAAAGAA 
AGAGATGAAG ATGATGAAGA TGGAGATGGC GATGGAGATG GAGCAACTGG AAAGAAGAAG 
AAAAAGAAGA AGAAGAAGAG AGGACCAAAA GTTCAAACAG ACCCTCCCTC AGTTCCAATA 
TGTGACCTGT ATCCTAATGG TGTATTTCCC AAAGGACAAG AATGCGAATA CCCACCCACA 
CAAGATGGGC GAACAGCTGC TTGGAGAACT ACAAGTGAAG AAAAGAAAGC ATTAGATCAG 
GCAAGTGAAG AGATTTGGAA TGATTTTCGA GAAGCTGCAG AAGCACATCG ACAAGTTAGA 
AAATACGTAA TGAGCTGGAT CAAGCCTGGG ATGACAATGA TAGAAATCTG TGAAAAGTTG 
GAAGACTGTT CACGCAAGTT AATAAAAGAG AATGGATTAA ATGCAGGCCT GGCATTTCCT 
ACTGGATGTT CTCTCAATAA TTGTGCTGCC CATTATACTC CCAATGCCGG TGACACAACA 
GTATTACAGT ATGATGACAT CTGTAAAATA GACTTTGGAA CACATATAAG TGGTAGGATT 
ATTGACTGTG CTTTTACTGT CACTTTTAAT CCCAAATATG ATACGTTATT AAAAGCTGTA 
AAAGATGCTA CTAACACTGG AATAAAGTGT GCTGGAATTG ATGTTCGTCT GTGTGATGTT 
GGTGAGGCCA TCCAAGAAGT TATGGAGTCC TATGAAGTTG AAATAGATGG GAAGACATAT 
CAAGTGAAAC CAATCCGTAA TCTAAATGGA CATTCAATTG GGCAATATAG AATACATGCT 
GGAAAAACAG TGCCGATTGT GAAAGGAGGG GAGGCAACAA GAATGGAGGA AGGAGAAGTA 
TATGCAATTG AAACCTTTGG TAGTACAGGA AAAGGTGTTG TTCATGATGA TATGGAATGT 
TCACATTACA TGAAAAATTT TGATGTTGGA CATGTGCCAA TAAGGCTTCC AAGAACAAAA 
CACTTGTTAA ATGTCATCAA TGAAAACTTT GGAACCCTTG CCTTCTGCCG CAGATGGCTG 
GATCGCTTGG GAGAAAGTAA ATACTTGATG GCTCTGAAGA ATCTGTGTGA CTTGGGCATT 
GTAGATCCAT ATCCACCATT ATGTGACATT AAAGGATCAT ATACAGCGCA ATTTGAACAT 
ACCATCCTGT TGCGTCCAAC ATGTAAAGAA GTTGTCAGCA GAGGAGATGA CTATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_006829.1
CDS	135..1571
Translation	MAGVEEVAASGSHLNGDLDPDDREEGAASTAEEAAKKKRRKKKKSKGPSAAGEQEPDKESGASVDEVARQLERSALEDKERDEDDEDGDGDGDGATGKKKKKKKKKRGPKVQTDPPSVPICDLYPNGVFPKGQECEYPPTQDGRTAAWRTTSEEKKALDQASEEIWNDFREAAEAHRQVRKYVMSWIKPGMTMIEICEKLEDCSRKLIKENGLNAGLAFPTGCSLNNCAAHYTPNAGDTTVLQYDDICKIDFGTHISGRIIDCAFTVTFNPKYDTLLKAVKDATNTGIKCAGIDVRLCDVGEAIQEVMESYEVEIDGKTYQVKPIRNLNGHSIGQYRIHAGKTVPIVKGGEATRMEEGEVYAIETFGSTGKGVVHDDMECSHYMKNFDVGHVPIRLPRTKHLLNVINENFGTLAFCRRWLDRLGESKYLMALKNLCDLGIVDPYPPLCDIKGSYTAQFEHTILLRPTCKEVVSRGDDY

Target ORF information:

RefSeq Version	NM_006838.3
Organism	Homo sapiens(human)
Definition	Homo sapiens methionyl aminopeptidase 2 (METAP2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006838.3

ORF Insert Sequence:

ATGGCGGGTG TGGAGGAGGT AGCGGCCTCC GGGAGCCACC TGAATGGCGA CCTGGATCCA 
GACGACAGGG AAGAAGGAGC TGCCTCTACG GCTGAGGAAG CAGCCAAGAA AAAAAGACGA 
AAGAAGAAGA AGAGCAAAGG GCCTTCTGCA GCAGGGGAAC AGGAACCTGA TAAAGAATCA 
GGAGCCTCAG TGGATGAAGT AGCAAGACAG TTGGAAAGAT CAGCATTGGA AGATAAAGAA 
AGAGATGAAG ATGATGAAGA TGGAGATGGC GATGGAGATG GAGCAACTGG AAAGAAGAAG 
AAAAAGAAGA AGAAGAAGAG AGGACCAAAA GTTCAAACAG ACCCTCCCTC AGTTCCAATA 
TGTGACCTGT ATCCTAATGG TGTATTTCCC AAAGGACAAG AATGCGAATA CCCACCCACA 
CAAGATGGGC GAACAGCTGC TTGGAGAACT ACAAGTGAAG AAAAGAAAGC ATTAGATCAG 
GCAAGTGAAG AGATTTGGAA TGATTTTCGA GAAGCTGCAG AAGCACATCG ACAAGTTAGA 
AAATACGTAA TGAGCTGGAT CAAGCCTGGG ATGACAATGA TAGAAATCTG TGAAAAGTTG 
GAAGACTGTT CACGCAAGTT AATAAAAGAG AATGGATTAA ATGCAGGCCT GGCATTTCCT 
ACTGGATGTT CTCTCAATAA TTGTGCTGCC CATTATACTC CCAATGCCGG TGACACAACA 
GTATTACAGT ATGATGACAT CTGTAAAATA GACTTTGGAA CACATATAAG TGGTAGGATT 
ATTGACTGTG CTTTTACTGT CACTTTTAAT CCCAAATATG ATACGTTATT AAAAGCTGTA 
AAAGATGCTA CTAACACTGG AATAAAGTGT GCTGGAATTG ATGTTCGTCT GTGTGATGTT 
GGTGAGGCCA TCCAAGAAGT TATGGAGTCC TATGAAGTTG AAATAGATGG GAAGACATAT 
CAAGTGAAAC CAATCCGTAA TCTAAATGGA CATTCAATTG GGCAATATAG AATACATGCT 
GGAAAAACAG TGCCGATTGT GAAAGGAGGG GAGGCAACAA GAATGGAGGA AGGAGAAGTA 
TATGCAATTG AAACCTTTGG TAGTACAGGA AAAGGTGTTG TTCATGATGA TATGGAATGT 
TCACATTACA TGAAAAATTT TGATGTTGGA CATGTGCCAA TAAGGCTTCC AAGAACAAAA 
CACTTGTTAA ATGTCATCAA TGAAAACTTT GGAACCCTTG CCTTCTGCCG CAGATGGCTG 
GATCGCTTGG GAGAAAGTAA ATACTTGATG GCTCTGAAGA ATCTGTGTGA CTTGGGCATT 
GTAGATCCAT ATCCACCATT ATGTGACATT AAAGGATCAT ATACAGCGCA ATTTGAACAT 
ACCATCCTGT TGCGTCCAAC ATGTAAAGAA GTTGTCAGCA GAGGAGATGA CTATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

METAP2 ( NM_006838.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_006829.1 Homo sapiens methionyl aminopeptidase 2 (METAP2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu30784
Clone ID Related Accession (Same CDS sequence)	NM_006838.3 , NM_006838.4
Accession Version	NM_006838.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1437bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-05
Organism	Homo sapiens(human)
Product	methionine aminopeptidase 2 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U13261.1 and AC018475.32. On Nov 22, 2018 this sequence version replaced NM_006838.3. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.27760.1, SRR1660805.235421.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000323666.10/ ENSP00000325312.5 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCGGGTG TGGAGGAGGT AGCGGCCTCC GGGAGCCACC TGAATGGCGA CCTGGATCCA 
GACGACAGGG AAGAAGGAGC TGCCTCTACG GCTGAGGAAG CAGCCAAGAA AAAAAGACGA 
AAGAAGAAGA AGAGCAAAGG GCCTTCTGCA GCAGGGGAAC AGGAACCTGA TAAAGAATCA 
GGAGCCTCAG TGGATGAAGT AGCAAGACAG TTGGAAAGAT CAGCATTGGA AGATAAAGAA 
AGAGATGAAG ATGATGAAGA TGGAGATGGC GATGGAGATG GAGCAACTGG AAAGAAGAAG 
AAAAAGAAGA AGAAGAAGAG AGGACCAAAA GTTCAAACAG ACCCTCCCTC AGTTCCAATA 
TGTGACCTGT ATCCTAATGG TGTATTTCCC AAAGGACAAG AATGCGAATA CCCACCCACA 
CAAGATGGGC GAACAGCTGC TTGGAGAACT ACAAGTGAAG AAAAGAAAGC ATTAGATCAG 
GCAAGTGAAG AGATTTGGAA TGATTTTCGA GAAGCTGCAG AAGCACATCG ACAAGTTAGA 
AAATACGTAA TGAGCTGGAT CAAGCCTGGG ATGACAATGA TAGAAATCTG TGAAAAGTTG 
GAAGACTGTT CACGCAAGTT AATAAAAGAG AATGGATTAA ATGCAGGCCT GGCATTTCCT 
ACTGGATGTT CTCTCAATAA TTGTGCTGCC CATTATACTC CCAATGCCGG TGACACAACA 
GTATTACAGT ATGATGACAT CTGTAAAATA GACTTTGGAA CACATATAAG TGGTAGGATT 
ATTGACTGTG CTTTTACTGT CACTTTTAAT CCCAAATATG ATACGTTATT AAAAGCTGTA 
AAAGATGCTA CTAACACTGG AATAAAGTGT GCTGGAATTG ATGTTCGTCT GTGTGATGTT 
GGTGAGGCCA TCCAAGAAGT TATGGAGTCC TATGAAGTTG AAATAGATGG GAAGACATAT 
CAAGTGAAAC CAATCCGTAA TCTAAATGGA CATTCAATTG GGCAATATAG AATACATGCT 
GGAAAAACAG TGCCGATTGT GAAAGGAGGG GAGGCAACAA GAATGGAGGA AGGAGAAGTA 
TATGCAATTG AAACCTTTGG TAGTACAGGA AAAGGTGTTG TTCATGATGA TATGGAATGT 
TCACATTACA TGAAAAATTT TGATGTTGGA CATGTGCCAA TAAGGCTTCC AAGAACAAAA 
CACTTGTTAA ATGTCATCAA TGAAAACTTT GGAACCCTTG CCTTCTGCCG CAGATGGCTG 
GATCGCTTGG GAGAAAGTAA ATACTTGATG GCTCTGAAGA ATCTGTGTGA CTTGGGCATT 
GTAGATCCAT ATCCACCATT ATGTGACATT AAAGGATCAT ATACAGCGCA ATTTGAACAT 
ACCATCCTGT TGCGTCCAAC ATGTAAAGAA GTTGTCAGCA GAGGAGATGA CTATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_006829.1
CDS	29..1465
Translation	MAGVEEVAASGSHLNGDLDPDDREEGAASTAEEAAKKKRRKKKKSKGPSAAGEQEPDKESGASVDEVARQLERSALEDKERDEDDEDGDGDGDGATGKKKKKKKKKRGPKVQTDPPSVPICDLYPNGVFPKGQECEYPPTQDGRTAAWRTTSEEKKALDQASEEIWNDFREAAEAHRQVRKYVMSWIKPGMTMIEICEKLEDCSRKLIKENGLNAGLAFPTGCSLNNCAAHYTPNAGDTTVLQYDDICKIDFGTHISGRIIDCAFTVTFNPKYDTLLKAVKDATNTGIKCAGIDVRLCDVGEAIQEVMESYEVEIDGKTYQVKPIRNLNGHSIGQYRIHAGKTVPIVKGGEATRMEEGEVYAIETFGSTGKGVVHDDMECSHYMKNFDVGHVPIRLPRTKHLLNVINENFGTLAFCRRWLDRLGESKYLMALKNLCDLGIVDPYPPLCDIKGSYTAQFEHTILLRPTCKEVVSRGDDY

Target ORF information:

RefSeq Version	NM_006838.4
Organism	Homo sapiens(human)
Definition	Homo sapiens methionyl aminopeptidase 2 (METAP2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006838.4

ORF Insert Sequence:

ATGGCGGGTG TGGAGGAGGT AGCGGCCTCC GGGAGCCACC TGAATGGCGA CCTGGATCCA 
GACGACAGGG AAGAAGGAGC TGCCTCTACG GCTGAGGAAG CAGCCAAGAA AAAAAGACGA 
AAGAAGAAGA AGAGCAAAGG GCCTTCTGCA GCAGGGGAAC AGGAACCTGA TAAAGAATCA 
GGAGCCTCAG TGGATGAAGT AGCAAGACAG TTGGAAAGAT CAGCATTGGA AGATAAAGAA 
AGAGATGAAG ATGATGAAGA TGGAGATGGC GATGGAGATG GAGCAACTGG AAAGAAGAAG 
AAAAAGAAGA AGAAGAAGAG AGGACCAAAA GTTCAAACAG ACCCTCCCTC AGTTCCAATA 
TGTGACCTGT ATCCTAATGG TGTATTTCCC AAAGGACAAG AATGCGAATA CCCACCCACA 
CAAGATGGGC GAACAGCTGC TTGGAGAACT ACAAGTGAAG AAAAGAAAGC ATTAGATCAG 
GCAAGTGAAG AGATTTGGAA TGATTTTCGA GAAGCTGCAG AAGCACATCG ACAAGTTAGA 
AAATACGTAA TGAGCTGGAT CAAGCCTGGG ATGACAATGA TAGAAATCTG TGAAAAGTTG 
GAAGACTGTT CACGCAAGTT AATAAAAGAG AATGGATTAA ATGCAGGCCT GGCATTTCCT 
ACTGGATGTT CTCTCAATAA TTGTGCTGCC CATTATACTC CCAATGCCGG TGACACAACA 
GTATTACAGT ATGATGACAT CTGTAAAATA GACTTTGGAA CACATATAAG TGGTAGGATT 
ATTGACTGTG CTTTTACTGT CACTTTTAAT CCCAAATATG ATACGTTATT AAAAGCTGTA 
AAAGATGCTA CTAACACTGG AATAAAGTGT GCTGGAATTG ATGTTCGTCT GTGTGATGTT 
GGTGAGGCCA TCCAAGAAGT TATGGAGTCC TATGAAGTTG AAATAGATGG GAAGACATAT 
CAAGTGAAAC CAATCCGTAA TCTAAATGGA CATTCAATTG GGCAATATAG AATACATGCT 
GGAAAAACAG TGCCGATTGT GAAAGGAGGG GAGGCAACAA GAATGGAGGA AGGAGAAGTA 
TATGCAATTG AAACCTTTGG TAGTACAGGA AAAGGTGTTG TTCATGATGA TATGGAATGT 
TCACATTACA TGAAAAATTT TGATGTTGGA CATGTGCCAA TAAGGCTTCC AAGAACAAAA 
CACTTGTTAA ATGTCATCAA TGAAAACTTT GGAACCCTTG CCTTCTGCCG CAGATGGCTG 
GATCGCTTGG GAGAAAGTAA ATACTTGATG GCTCTGAAGA ATCTGTGTGA CTTGGGCATT 
GTAGATCCAT ATCCACCATT ATGTGACATT AAAGGATCAT ATACAGCGCA ATTTGAACAT 
ACCATCCTGT TGCGTCCAAC ATGTAAAGAA GTTGTCAGCA GAGGAGATGA CTATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

METAP2 ( NM_001317183.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001304112.1 Homo sapiens methionyl aminopeptidase 2 (METAP2), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu101532
Clone ID Related Accession (Same CDS sequence)	NM_001317183.1 , NM_001317183.2
Accession Version	NM_001317183.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1368bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-09
Organism	Homo sapiens(human)
Product	methionine aminopeptidase 2 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DW409106.1, AK091730.1 and AC018475.32. Transcript Variant: This variant (3) uses an alternate in-frame splice junction and lacks an alternate in-frame exon compared to variant 1. The resulting isoform (3) has the same N- and C-termini but is shorter compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK091730.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2144335 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGGTG TGGAGGAGGT AGCGGCCTCC GGGAGCCACC TGAATGGCGA CCTGGATCCA 
GACGACAGGG AAGAAGGAGC TGCCTCTACG GCTGAGGAAG CAGCCAAGAA AAAAAGACGA 
AAGAAGAAGA AGAGCAAAGG GCCTTCTGCA GGGGAACAGG AACCTGATAA AGAATCAGGA 
GCCTCAGTGG ATGAAGTAGC AAGACAGTTG GAAAGATCAG CATTGGAAGA TAAAGAAAGA 
GATGAAGATG ATGAAGCAAA AGTTCAAACA GACCCTCCCT CAGTTCCAAT ATGTGACCTG 
TATCCTAATG GTGTATTTCC CAAAGGACAA GAATGCGAAT ACCCACCCAC ACAAGATGGG 
CGAACAGCTG CTTGGAGAAC TACAAGTGAA GAAAAGAAAG CATTAGATCA GGCAAGTGAA 
GAGATTTGGA ATGATTTTCG AGAAGCTGCA GAAGCACATC GACAAGTTAG AAAATACGTA 
ATGAGCTGGA TCAAGCCTGG GATGACAATG ATAGAAATCT GTGAAAAGTT GGAAGACTGT 
TCACGCAAGT TAATAAAAGA GAATGGATTA AATGCAGGCC TGGCATTTCC TACTGGATGT 
TCTCTCAATA ATTGTGCTGC CCATTATACT CCCAATGCCG GTGACACAAC AGTATTACAG 
TATGATGACA TCTGTAAAAT AGACTTTGGA ACACATATAA GTGGTAGGAT TATTGACTGT 
GCTTTTACTG TCACTTTTAA TCCCAAATAT GATACGTTAT TAAAAGCTGT AAAAGATGCT 
ACTAACACTG GAATAAAGTG TGCTGGAATT GATGTTCGTC TGTGTGATGT TGGTGAGGCC 
ATCCAAGAAG TTATGGAGTC CTATGAAGTT GAAATAGATG GGAAGACATA TCAAGTGAAA 
CCAATCCGTA ATCTAAATGG ACATTCAATT GGGCAATATA GAATACATGC TGGAAAAACA 
GTGCCGATTG TGAAAGGAGG GGAGGCAACA AGAATGGAGG AAGGAGAAGT ATATGCAATT 
GAAACCTTTG GTAGTACAGG AAAAGGTGTT GTTCATGATG ATATGGAATG TTCACATTAC 
ATGAAAAATT TTGATGTTGG ACATGTGCCA ATAAGGCTTC CAAGAACAAA ACACTTGTTA 
AATGTCATCA ATGAAAACTT TGGAACCCTT GCCTTCTGCC GCAGATGGCT GGATCGCTTG 
GGAGAAAGTA AATACTTGAT GGCTCTGAAG AATCTGTGTG ACTTGGGCAT TGTAGATCCA 
TATCCACCAT TATGTGACAT TAAAGGATCA TATACAGCGC AATTTGAACA TACCATCCTG 
TTGCGTCCAA CATGTAAAGA AGTTGTCAGC AGAGGAGATG ACTATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001304112.1
CDS	135..1502
Translation	MAGVEEVAASGSHLNGDLDPDDREEGAASTAEEAAKKKRRKKKKSKGPSAGEQEPDKESGASVDEVARQLERSALEDKERDEDDEAKVQTDPPSVPICDLYPNGVFPKGQECEYPPTQDGRTAAWRTTSEEKKALDQASEEIWNDFREAAEAHRQVRKYVMSWIKPGMTMIEICEKLEDCSRKLIKENGLNAGLAFPTGCSLNNCAAHYTPNAGDTTVLQYDDICKIDFGTHISGRIIDCAFTVTFNPKYDTLLKAVKDATNTGIKCAGIDVRLCDVGEAIQEVMESYEVEIDGKTYQVKPIRNLNGHSIGQYRIHAGKTVPIVKGGEATRMEEGEVYAIETFGSTGKGVVHDDMECSHYMKNFDVGHVPIRLPRTKHLLNVINENFGTLAFCRRWLDRLGESKYLMALKNLCDLGIVDPYPPLCDIKGSYTAQFEHTILLRPTCKEVVSRGDDY

Target ORF information:

RefSeq Version	NM_001317183.1
Organism	Homo sapiens(human)
Definition	Homo sapiens methionyl aminopeptidase 2 (METAP2), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001317183.1

ORF Insert Sequence:

ATGGCGGGTG TGGAGGAGGT AGCGGCCTCC GGGAGCCACC TGAATGGCGA CCTGGATCCA 
GACGACAGGG AAGAAGGAGC TGCCTCTACG GCTGAGGAAG CAGCCAAGAA AAAAAGACGA 
AAGAAGAAGA AGAGCAAAGG GCCTTCTGCA GGGGAACAGG AACCTGATAA AGAATCAGGA 
GCCTCAGTGG ATGAAGTAGC AAGACAGTTG GAAAGATCAG CATTGGAAGA TAAAGAAAGA 
GATGAAGATG ATGAAGCAAA AGTTCAAACA GACCCTCCCT CAGTTCCAAT ATGTGACCTG 
TATCCTAATG GTGTATTTCC CAAAGGACAA GAATGCGAAT ACCCACCCAC ACAAGATGGG 
CGAACAGCTG CTTGGAGAAC TACAAGTGAA GAAAAGAAAG CATTAGATCA GGCAAGTGAA 
GAGATTTGGA ATGATTTTCG AGAAGCTGCA GAAGCACATC GACAAGTTAG AAAATACGTA 
ATGAGCTGGA TCAAGCCTGG GATGACAATG ATAGAAATCT GTGAAAAGTT GGAAGACTGT 
TCACGCAAGT TAATAAAAGA GAATGGATTA AATGCAGGCC TGGCATTTCC TACTGGATGT 
TCTCTCAATA ATTGTGCTGC CCATTATACT CCCAATGCCG GTGACACAAC AGTATTACAG 
TATGATGACA TCTGTAAAAT AGACTTTGGA ACACATATAA GTGGTAGGAT TATTGACTGT 
GCTTTTACTG TCACTTTTAA TCCCAAATAT GATACGTTAT TAAAAGCTGT AAAAGATGCT 
ACTAACACTG GAATAAAGTG TGCTGGAATT GATGTTCGTC TGTGTGATGT TGGTGAGGCC 
ATCCAAGAAG TTATGGAGTC CTATGAAGTT GAAATAGATG GGAAGACATA TCAAGTGAAA 
CCAATCCGTA ATCTAAATGG ACATTCAATT GGGCAATATA GAATACATGC TGGAAAAACA 
GTGCCGATTG TGAAAGGAGG GGAGGCAACA AGAATGGAGG AAGGAGAAGT ATATGCAATT 
GAAACCTTTG GTAGTACAGG AAAAGGTGTT GTTCATGATG ATATGGAATG TTCACATTAC 
ATGAAAAATT TTGATGTTGG ACATGTGCCA ATAAGGCTTC CAAGAACAAA ACACTTGTTA 
AATGTCATCA ATGAAAACTT TGGAACCCTT GCCTTCTGCC GCAGATGGCT GGATCGCTTG 
GGAGAAAGTA AATACTTGAT GGCTCTGAAG AATCTGTGTG ACTTGGGCAT TGTAGATCCA 
TATCCACCAT TATGTGACAT TAAAGGATCA TATACAGCGC AATTTGAACA TACCATCCTG 
TTGCGTCCAA CATGTAAAGA AGTTGTCAGC AGAGGAGATG ACTATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

METAP2 ( NM_001317183.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001304112.1 Homo sapiens methionyl aminopeptidase 2 (METAP2), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu101532
Clone ID Related Accession (Same CDS sequence)	NM_001317183.1 , NM_001317183.2
Accession Version	NM_001317183.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1368bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-06
Organism	Homo sapiens(human)
Product	methionine aminopeptidase 2 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DW409106.1, AK091730.1 and AC018475.32. On May 31, 2019 this sequence version replaced NM_001317183.1. Transcript Variant: This variant (3) uses an alternate in-frame splice junction and lacks an alternate in-frame exon compared to variant 1. The resulting isoform (3) has the same N- and C-termini but is shorter compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK091730.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2144335 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGGTG TGGAGGAGGT AGCGGCCTCC GGGAGCCACC TGAATGGCGA CCTGGATCCA 
GACGACAGGG AAGAAGGAGC TGCCTCTACG GCTGAGGAAG CAGCCAAGAA AAAAAGACGA 
AAGAAGAAGA AGAGCAAAGG GCCTTCTGCA GGGGAACAGG AACCTGATAA AGAATCAGGA 
GCCTCAGTGG ATGAAGTAGC AAGACAGTTG GAAAGATCAG CATTGGAAGA TAAAGAAAGA 
GATGAAGATG ATGAAGCAAA AGTTCAAACA GACCCTCCCT CAGTTCCAAT ATGTGACCTG 
TATCCTAATG GTGTATTTCC CAAAGGACAA GAATGCGAAT ACCCACCCAC ACAAGATGGG 
CGAACAGCTG CTTGGAGAAC TACAAGTGAA GAAAAGAAAG CATTAGATCA GGCAAGTGAA 
GAGATTTGGA ATGATTTTCG AGAAGCTGCA GAAGCACATC GACAAGTTAG AAAATACGTA 
ATGAGCTGGA TCAAGCCTGG GATGACAATG ATAGAAATCT GTGAAAAGTT GGAAGACTGT 
TCACGCAAGT TAATAAAAGA GAATGGATTA AATGCAGGCC TGGCATTTCC TACTGGATGT 
TCTCTCAATA ATTGTGCTGC CCATTATACT CCCAATGCCG GTGACACAAC AGTATTACAG 
TATGATGACA TCTGTAAAAT AGACTTTGGA ACACATATAA GTGGTAGGAT TATTGACTGT 
GCTTTTACTG TCACTTTTAA TCCCAAATAT GATACGTTAT TAAAAGCTGT AAAAGATGCT 
ACTAACACTG GAATAAAGTG TGCTGGAATT GATGTTCGTC TGTGTGATGT TGGTGAGGCC 
ATCCAAGAAG TTATGGAGTC CTATGAAGTT GAAATAGATG GGAAGACATA TCAAGTGAAA 
CCAATCCGTA ATCTAAATGG ACATTCAATT GGGCAATATA GAATACATGC TGGAAAAACA 
GTGCCGATTG TGAAAGGAGG GGAGGCAACA AGAATGGAGG AAGGAGAAGT ATATGCAATT 
GAAACCTTTG GTAGTACAGG AAAAGGTGTT GTTCATGATG ATATGGAATG TTCACATTAC 
ATGAAAAATT TTGATGTTGG ACATGTGCCA ATAAGGCTTC CAAGAACAAA ACACTTGTTA 
AATGTCATCA ATGAAAACTT TGGAACCCTT GCCTTCTGCC GCAGATGGCT GGATCGCTTG 
GGAGAAAGTA AATACTTGAT GGCTCTGAAG AATCTGTGTG ACTTGGGCAT TGTAGATCCA 
TATCCACCAT TATGTGACAT TAAAGGATCA TATACAGCGC AATTTGAACA TACCATCCTG 
TTGCGTCCAA CATGTAAAGA AGTTGTCAGC AGAGGAGATG ACTATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001304112.1
CDS	29..1396
Translation	MAGVEEVAASGSHLNGDLDPDDREEGAASTAEEAAKKKRRKKKKSKGPSAGEQEPDKESGASVDEVARQLERSALEDKERDEDDEAKVQTDPPSVPICDLYPNGVFPKGQECEYPPTQDGRTAAWRTTSEEKKALDQASEEIWNDFREAAEAHRQVRKYVMSWIKPGMTMIEICEKLEDCSRKLIKENGLNAGLAFPTGCSLNNCAAHYTPNAGDTTVLQYDDICKIDFGTHISGRIIDCAFTVTFNPKYDTLLKAVKDATNTGIKCAGIDVRLCDVGEAIQEVMESYEVEIDGKTYQVKPIRNLNGHSIGQYRIHAGKTVPIVKGGEATRMEEGEVYAIETFGSTGKGVVHDDMECSHYMKNFDVGHVPIRLPRTKHLLNVINENFGTLAFCRRWLDRLGESKYLMALKNLCDLGIVDPYPPLCDIKGSYTAQFEHTILLRPTCKEVVSRGDDY

Target ORF information:

RefSeq Version	NM_001317183.2
Organism	Homo sapiens(human)
Definition	Homo sapiens methionyl aminopeptidase 2 (METAP2), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001317183.2

ORF Insert Sequence:

ATGGCGGGTG TGGAGGAGGT AGCGGCCTCC GGGAGCCACC TGAATGGCGA CCTGGATCCA 
GACGACAGGG AAGAAGGAGC TGCCTCTACG GCTGAGGAAG CAGCCAAGAA AAAAAGACGA 
AAGAAGAAGA AGAGCAAAGG GCCTTCTGCA GGGGAACAGG AACCTGATAA AGAATCAGGA 
GCCTCAGTGG ATGAAGTAGC AAGACAGTTG GAAAGATCAG CATTGGAAGA TAAAGAAAGA 
GATGAAGATG ATGAAGCAAA AGTTCAAACA GACCCTCCCT CAGTTCCAAT ATGTGACCTG 
TATCCTAATG GTGTATTTCC CAAAGGACAA GAATGCGAAT ACCCACCCAC ACAAGATGGG 
CGAACAGCTG CTTGGAGAAC TACAAGTGAA GAAAAGAAAG CATTAGATCA GGCAAGTGAA 
GAGATTTGGA ATGATTTTCG AGAAGCTGCA GAAGCACATC GACAAGTTAG AAAATACGTA 
ATGAGCTGGA TCAAGCCTGG GATGACAATG ATAGAAATCT GTGAAAAGTT GGAAGACTGT 
TCACGCAAGT TAATAAAAGA GAATGGATTA AATGCAGGCC TGGCATTTCC TACTGGATGT 
TCTCTCAATA ATTGTGCTGC CCATTATACT CCCAATGCCG GTGACACAAC AGTATTACAG 
TATGATGACA TCTGTAAAAT AGACTTTGGA ACACATATAA GTGGTAGGAT TATTGACTGT 
GCTTTTACTG TCACTTTTAA TCCCAAATAT GATACGTTAT TAAAAGCTGT AAAAGATGCT 
ACTAACACTG GAATAAAGTG TGCTGGAATT GATGTTCGTC TGTGTGATGT TGGTGAGGCC 
ATCCAAGAAG TTATGGAGTC CTATGAAGTT GAAATAGATG GGAAGACATA TCAAGTGAAA 
CCAATCCGTA ATCTAAATGG ACATTCAATT GGGCAATATA GAATACATGC TGGAAAAACA 
GTGCCGATTG TGAAAGGAGG GGAGGCAACA AGAATGGAGG AAGGAGAAGT ATATGCAATT 
GAAACCTTTG GTAGTACAGG AAAAGGTGTT GTTCATGATG ATATGGAATG TTCACATTAC 
ATGAAAAATT TTGATGTTGG ACATGTGCCA ATAAGGCTTC CAAGAACAAA ACACTTGTTA 
AATGTCATCA ATGAAAACTT TGGAACCCTT GCCTTCTGCC GCAGATGGCT GGATCGCTTG 
GGAGAAAGTA AATACTTGAT GGCTCTGAAG AATCTGTGTG ACTTGGGCAT TGTAGATCCA 
TATCCACCAT TATGTGACAT TAAAGGATCA TATACAGCGC AATTTGAACA TACCATCCTG 
TTGCGTCCAA CATGTAAAGA AGTTGTCAGC AGAGGAGATG ACTATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

METAP2 ( NM_001317182.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001304111.1 Homo sapiens methionyl aminopeptidase 2 (METAP2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu101443
Clone ID Related Accession (Same CDS sequence)	NM_001317182.1 , NM_001317182.2
Accession Version	NM_001317182.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1368bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-09
Organism	Homo sapiens(human)
Product	methionine aminopeptidase 2 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DW409106.1, AK300836.1 and AC018475.32. Transcript Variant: This variant (2) uses an alternate in-frame splice junction compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK300836.1, SRR1803614.176514.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGGTG TGGAGGAGGT AGCGGCCTCC GGGAGCCACC TGAATGGCGA CCTGGATCCA 
GACGACAGGG AAGAAGGAGC TGCCTCTACG GCTGAGGAAG CAGCCAAGAA AAAAAGACGA 
AAGAAGAAGA AGAGCAAAGG GCCTTCTGCA GCAGGGGAAC AGGAACCTGA TAAAGAATCA 
GGAGCCTCAG TGGATGAAGT AGCAAGACAG TTGGAAAGAT CAGCATTGGA AGATAAAGAA 
AGAGATGAAG ATGATGAAGA TGGAGATGGC GATGGAGATG GAGCAACTGG AAAGAAGAAG 
AAAAAGAAGA AGAAGAAGAG AGGACGACAA GAATGCGAAT ACCCACCCAC ACAAGATGGG 
CGAACAGCTG CTTGGAGAAC TACAAGTGAA GAAAAGAAAG CATTAGATCA GGCAAGTGAA 
GAGATTTGGA ATGATTTTCG AGAAGCTGCA GAAGCACATC GACAAGTTAG AAAATACGTA 
ATGAGCTGGA TCAAGCCTGG GATGACAATG ATAGAAATCT GTGAAAAGTT GGAAGACTGT 
TCACGCAAGT TAATAAAAGA GAATGGATTA AATGCAGGCC TGGCATTTCC TACTGGATGT 
TCTCTCAATA ATTGTGCTGC CCATTATACT CCCAATGCCG GTGACACAAC AGTATTACAG 
TATGATGACA TCTGTAAAAT AGACTTTGGA ACACATATAA GTGGTAGGAT TATTGACTGT 
GCTTTTACTG TCACTTTTAA TCCCAAATAT GATACGTTAT TAAAAGCTGT AAAAGATGCT 
ACTAACACTG GAATAAAGTG TGCTGGAATT GATGTTCGTC TGTGTGATGT TGGTGAGGCC 
ATCCAAGAAG TTATGGAGTC CTATGAAGTT GAAATAGATG GGAAGACATA TCAAGTGAAA 
CCAATCCGTA ATCTAAATGG ACATTCAATT GGGCAATATA GAATACATGC TGGAAAAACA 
GTGCCGATTG TGAAAGGAGG GGAGGCAACA AGAATGGAGG AAGGAGAAGT ATATGCAATT 
GAAACCTTTG GTAGTACAGG AAAAGGTGTT GTTCATGATG ATATGGAATG TTCACATTAC 
ATGAAAAATT TTGATGTTGG ACATGTGCCA ATAAGGCTTC CAAGAACAAA ACACTTGTTA 
AATGTCATCA ATGAAAACTT TGGAACCCTT GCCTTCTGCC GCAGATGGCT GGATCGCTTG 
GGAGAAAGTA AATACTTGAT GGCTCTGAAG AATCTGTGTG ACTTGGGCAT TGTAGATCCA 
TATCCACCAT TATGTGACAT TAAAGGATCA TATACAGCGC AATTTGAACA TACCATCCTG 
TTGCGTCCAA CATGTAAAGA AGTTGTCAGC AGAGGAGATG ACTATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001304111.1
CDS	135..1502
Translation	MAGVEEVAASGSHLNGDLDPDDREEGAASTAEEAAKKKRRKKKKSKGPSAAGEQEPDKESGASVDEVARQLERSALEDKERDEDDEDGDGDGDGATGKKKKKKKKKRGRQECEYPPTQDGRTAAWRTTSEEKKALDQASEEIWNDFREAAEAHRQVRKYVMSWIKPGMTMIEICEKLEDCSRKLIKENGLNAGLAFPTGCSLNNCAAHYTPNAGDTTVLQYDDICKIDFGTHISGRIIDCAFTVTFNPKYDTLLKAVKDATNTGIKCAGIDVRLCDVGEAIQEVMESYEVEIDGKTYQVKPIRNLNGHSIGQYRIHAGKTVPIVKGGEATRMEEGEVYAIETFGSTGKGVVHDDMECSHYMKNFDVGHVPIRLPRTKHLLNVINENFGTLAFCRRWLDRLGESKYLMALKNLCDLGIVDPYPPLCDIKGSYTAQFEHTILLRPTCKEVVSRGDDY

Target ORF information:

RefSeq Version	NM_001317182.1
Organism	Homo sapiens(human)
Definition	Homo sapiens methionyl aminopeptidase 2 (METAP2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001317182.1

ORF Insert Sequence:

ATGGCGGGTG TGGAGGAGGT AGCGGCCTCC GGGAGCCACC TGAATGGCGA CCTGGATCCA 
GACGACAGGG AAGAAGGAGC TGCCTCTACG GCTGAGGAAG CAGCCAAGAA AAAAAGACGA 
AAGAAGAAGA AGAGCAAAGG GCCTTCTGCA GCAGGGGAAC AGGAACCTGA TAAAGAATCA 
GGAGCCTCAG TGGATGAAGT AGCAAGACAG TTGGAAAGAT CAGCATTGGA AGATAAAGAA 
AGAGATGAAG ATGATGAAGA TGGAGATGGC GATGGAGATG GAGCAACTGG AAAGAAGAAG 
AAAAAGAAGA AGAAGAAGAG AGGACGACAA GAATGCGAAT ACCCACCCAC ACAAGATGGG 
CGAACAGCTG CTTGGAGAAC TACAAGTGAA GAAAAGAAAG CATTAGATCA GGCAAGTGAA 
GAGATTTGGA ATGATTTTCG AGAAGCTGCA GAAGCACATC GACAAGTTAG AAAATACGTA 
ATGAGCTGGA TCAAGCCTGG GATGACAATG ATAGAAATCT GTGAAAAGTT GGAAGACTGT 
TCACGCAAGT TAATAAAAGA GAATGGATTA AATGCAGGCC TGGCATTTCC TACTGGATGT 
TCTCTCAATA ATTGTGCTGC CCATTATACT CCCAATGCCG GTGACACAAC AGTATTACAG 
TATGATGACA TCTGTAAAAT AGACTTTGGA ACACATATAA GTGGTAGGAT TATTGACTGT 
GCTTTTACTG TCACTTTTAA TCCCAAATAT GATACGTTAT TAAAAGCTGT AAAAGATGCT 
ACTAACACTG GAATAAAGTG TGCTGGAATT GATGTTCGTC TGTGTGATGT TGGTGAGGCC 
ATCCAAGAAG TTATGGAGTC CTATGAAGTT GAAATAGATG GGAAGACATA TCAAGTGAAA 
CCAATCCGTA ATCTAAATGG ACATTCAATT GGGCAATATA GAATACATGC TGGAAAAACA 
GTGCCGATTG TGAAAGGAGG GGAGGCAACA AGAATGGAGG AAGGAGAAGT ATATGCAATT 
GAAACCTTTG GTAGTACAGG AAAAGGTGTT GTTCATGATG ATATGGAATG TTCACATTAC 
ATGAAAAATT TTGATGTTGG ACATGTGCCA ATAAGGCTTC CAAGAACAAA ACACTTGTTA 
AATGTCATCA ATGAAAACTT TGGAACCCTT GCCTTCTGCC GCAGATGGCT GGATCGCTTG 
GGAGAAAGTA AATACTTGAT GGCTCTGAAG AATCTGTGTG ACTTGGGCAT TGTAGATCCA 
TATCCACCAT TATGTGACAT TAAAGGATCA TATACAGCGC AATTTGAACA TACCATCCTG 
TTGCGTCCAA CATGTAAAGA AGTTGTCAGC AGAGGAGATG ACTATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

METAP2 ( NM_001317182.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001304111.1 Homo sapiens methionyl aminopeptidase 2 (METAP2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu101443
Clone ID Related Accession (Same CDS sequence)	NM_001317182.1 , NM_001317182.2
Accession Version	NM_001317182.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1368bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-06
Organism	Homo sapiens(human)
Product	methionine aminopeptidase 2 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK300836.1 and AC018475.32. On May 31, 2019 this sequence version replaced NM_001317182.1. Transcript Variant: This variant (2) uses an alternate in-frame splice junction compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK300836.1, SRR1803614.176514.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGGTG TGGAGGAGGT AGCGGCCTCC GGGAGCCACC TGAATGGCGA CCTGGATCCA 
GACGACAGGG AAGAAGGAGC TGCCTCTACG GCTGAGGAAG CAGCCAAGAA AAAAAGACGA 
AAGAAGAAGA AGAGCAAAGG GCCTTCTGCA GCAGGGGAAC AGGAACCTGA TAAAGAATCA 
GGAGCCTCAG TGGATGAAGT AGCAAGACAG TTGGAAAGAT CAGCATTGGA AGATAAAGAA 
AGAGATGAAG ATGATGAAGA TGGAGATGGC GATGGAGATG GAGCAACTGG AAAGAAGAAG 
AAAAAGAAGA AGAAGAAGAG AGGACGACAA GAATGCGAAT ACCCACCCAC ACAAGATGGG 
CGAACAGCTG CTTGGAGAAC TACAAGTGAA GAAAAGAAAG CATTAGATCA GGCAAGTGAA 
GAGATTTGGA ATGATTTTCG AGAAGCTGCA GAAGCACATC GACAAGTTAG AAAATACGTA 
ATGAGCTGGA TCAAGCCTGG GATGACAATG ATAGAAATCT GTGAAAAGTT GGAAGACTGT 
TCACGCAAGT TAATAAAAGA GAATGGATTA AATGCAGGCC TGGCATTTCC TACTGGATGT 
TCTCTCAATA ATTGTGCTGC CCATTATACT CCCAATGCCG GTGACACAAC AGTATTACAG 
TATGATGACA TCTGTAAAAT AGACTTTGGA ACACATATAA GTGGTAGGAT TATTGACTGT 
GCTTTTACTG TCACTTTTAA TCCCAAATAT GATACGTTAT TAAAAGCTGT AAAAGATGCT 
ACTAACACTG GAATAAAGTG TGCTGGAATT GATGTTCGTC TGTGTGATGT TGGTGAGGCC 
ATCCAAGAAG TTATGGAGTC CTATGAAGTT GAAATAGATG GGAAGACATA TCAAGTGAAA 
CCAATCCGTA ATCTAAATGG ACATTCAATT GGGCAATATA GAATACATGC TGGAAAAACA 
GTGCCGATTG TGAAAGGAGG GGAGGCAACA AGAATGGAGG AAGGAGAAGT ATATGCAATT 
GAAACCTTTG GTAGTACAGG AAAAGGTGTT GTTCATGATG ATATGGAATG TTCACATTAC 
ATGAAAAATT TTGATGTTGG ACATGTGCCA ATAAGGCTTC CAAGAACAAA ACACTTGTTA 
AATGTCATCA ATGAAAACTT TGGAACCCTT GCCTTCTGCC GCAGATGGCT GGATCGCTTG 
GGAGAAAGTA AATACTTGAT GGCTCTGAAG AATCTGTGTG ACTTGGGCAT TGTAGATCCA 
TATCCACCAT TATGTGACAT TAAAGGATCA TATACAGCGC AATTTGAACA TACCATCCTG 
TTGCGTCCAA CATGTAAAGA AGTTGTCAGC AGAGGAGATG ACTATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001304111.1
CDS	29..1396
Translation	MAGVEEVAASGSHLNGDLDPDDREEGAASTAEEAAKKKRRKKKKSKGPSAAGEQEPDKESGASVDEVARQLERSALEDKERDEDDEDGDGDGDGATGKKKKKKKKKRGRQECEYPPTQDGRTAAWRTTSEEKKALDQASEEIWNDFREAAEAHRQVRKYVMSWIKPGMTMIEICEKLEDCSRKLIKENGLNAGLAFPTGCSLNNCAAHYTPNAGDTTVLQYDDICKIDFGTHISGRIIDCAFTVTFNPKYDTLLKAVKDATNTGIKCAGIDVRLCDVGEAIQEVMESYEVEIDGKTYQVKPIRNLNGHSIGQYRIHAGKTVPIVKGGEATRMEEGEVYAIETFGSTGKGVVHDDMECSHYMKNFDVGHVPIRLPRTKHLLNVINENFGTLAFCRRWLDRLGESKYLMALKNLCDLGIVDPYPPLCDIKGSYTAQFEHTILLRPTCKEVVSRGDDY

Target ORF information:

RefSeq Version	NM_001317182.2
Organism	Homo sapiens(human)
Definition	Homo sapiens methionyl aminopeptidase 2 (METAP2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001317182.2

ORF Insert Sequence:

ATGGCGGGTG TGGAGGAGGT AGCGGCCTCC GGGAGCCACC TGAATGGCGA CCTGGATCCA 
GACGACAGGG AAGAAGGAGC TGCCTCTACG GCTGAGGAAG CAGCCAAGAA AAAAAGACGA 
AAGAAGAAGA AGAGCAAAGG GCCTTCTGCA GCAGGGGAAC AGGAACCTGA TAAAGAATCA 
GGAGCCTCAG TGGATGAAGT AGCAAGACAG TTGGAAAGAT CAGCATTGGA AGATAAAGAA 
AGAGATGAAG ATGATGAAGA TGGAGATGGC GATGGAGATG GAGCAACTGG AAAGAAGAAG 
AAAAAGAAGA AGAAGAAGAG AGGACGACAA GAATGCGAAT ACCCACCCAC ACAAGATGGG 
CGAACAGCTG CTTGGAGAAC TACAAGTGAA GAAAAGAAAG CATTAGATCA GGCAAGTGAA 
GAGATTTGGA ATGATTTTCG AGAAGCTGCA GAAGCACATC GACAAGTTAG AAAATACGTA 
ATGAGCTGGA TCAAGCCTGG GATGACAATG ATAGAAATCT GTGAAAAGTT GGAAGACTGT 
TCACGCAAGT TAATAAAAGA GAATGGATTA AATGCAGGCC TGGCATTTCC TACTGGATGT 
TCTCTCAATA ATTGTGCTGC CCATTATACT CCCAATGCCG GTGACACAAC AGTATTACAG 
TATGATGACA TCTGTAAAAT AGACTTTGGA ACACATATAA GTGGTAGGAT TATTGACTGT 
GCTTTTACTG TCACTTTTAA TCCCAAATAT GATACGTTAT TAAAAGCTGT AAAAGATGCT 
ACTAACACTG GAATAAAGTG TGCTGGAATT GATGTTCGTC TGTGTGATGT TGGTGAGGCC 
ATCCAAGAAG TTATGGAGTC CTATGAAGTT GAAATAGATG GGAAGACATA TCAAGTGAAA 
CCAATCCGTA ATCTAAATGG ACATTCAATT GGGCAATATA GAATACATGC TGGAAAAACA 
GTGCCGATTG TGAAAGGAGG GGAGGCAACA AGAATGGAGG AAGGAGAAGT ATATGCAATT 
GAAACCTTTG GTAGTACAGG AAAAGGTGTT GTTCATGATG ATATGGAATG TTCACATTAC 
ATGAAAAATT TTGATGTTGG ACATGTGCCA ATAAGGCTTC CAAGAACAAA ACACTTGTTA 
AATGTCATCA ATGAAAACTT TGGAACCCTT GCCTTCTGCC GCAGATGGCT GGATCGCTTG 
GGAGAAAGTA AATACTTGAT GGCTCTGAAG AATCTGTGTG ACTTGGGCAT TGTAGATCCA 
TATCCACCAT TATGTGACAT TAAAGGATCA TATACAGCGC AATTTGAACA TACCATCCTG 
TTGCGTCCAA CATGTAAAGA AGTTGTCAGC AGAGGAGATG ACTATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

METAP2 ( NM_001330246.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001317175.1 Homo sapiens methionyl aminopeptidase 2 (METAP2), transcript variant 5, mRNA.

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CloneID	OHu46263
Clone ID Related Accession (Same CDS sequence)	NM_001330246.1 , NM_001330246.2
Accession Version	NM_001330246.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1434bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-09
Organism	Homo sapiens(human)
Product	methionine aminopeptidase 2 isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DW409106.1, U13261.1, AK091730.1, AC018475.32 and CA450598.1. On Aug 20, 2016 this sequence version replaced XM_005268583.3. Transcript Variant: This variant (5) uses an alternate in-frame splice junction in the 5' coding region compared to variant 1. The resulting isoform (4) has the same N- and C-termini but is one amino acid shorter compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.205314.1, SRR1803615.114090.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGGTG TGGAGGAGGT AGCGGCCTCC GGGAGCCACC TGAATGGCGA CCTGGATCCA 
GACGACAGGG AAGAAGGAGC TGCCTCTACG GCTGAGGAAG CAGCCAAGAA AAAAAGACGA 
AAGAAGAAGA AGAGCAAAGG GCCTTCTGCA GGGGAACAGG AACCTGATAA AGAATCAGGA 
GCCTCAGTGG ATGAAGTAGC AAGACAGTTG GAAAGATCAG CATTGGAAGA TAAAGAAAGA 
GATGAAGATG ATGAAGATGG AGATGGCGAT GGAGATGGAG CAACTGGAAA GAAGAAGAAA 
AAGAAGAAGA AGAAGAGAGG ACCAAAAGTT CAAACAGACC CTCCCTCAGT TCCAATATGT 
GACCTGTATC CTAATGGTGT ATTTCCCAAA GGACAAGAAT GCGAATACCC ACCCACACAA 
GATGGGCGAA CAGCTGCTTG GAGAACTACA AGTGAAGAAA AGAAAGCATT AGATCAGGCA 
AGTGAAGAGA TTTGGAATGA TTTTCGAGAA GCTGCAGAAG CACATCGACA AGTTAGAAAA 
TACGTAATGA GCTGGATCAA GCCTGGGATG ACAATGATAG AAATCTGTGA AAAGTTGGAA 
GACTGTTCAC GCAAGTTAAT AAAAGAGAAT GGATTAAATG CAGGCCTGGC ATTTCCTACT 
GGATGTTCTC TCAATAATTG TGCTGCCCAT TATACTCCCA ATGCCGGTGA CACAACAGTA 
TTACAGTATG ATGACATCTG TAAAATAGAC TTTGGAACAC ATATAAGTGG TAGGATTATT 
GACTGTGCTT TTACTGTCAC TTTTAATCCC AAATATGATA CGTTATTAAA AGCTGTAAAA 
GATGCTACTA ACACTGGAAT AAAGTGTGCT GGAATTGATG TTCGTCTGTG TGATGTTGGT 
GAGGCCATCC AAGAAGTTAT GGAGTCCTAT GAAGTTGAAA TAGATGGGAA GACATATCAA 
GTGAAACCAA TCCGTAATCT AAATGGACAT TCAATTGGGC AATATAGAAT ACATGCTGGA 
AAAACAGTGC CGATTGTGAA AGGAGGGGAG GCAACAAGAA TGGAGGAAGG AGAAGTATAT 
GCAATTGAAA CCTTTGGTAG TACAGGAAAA GGTGTTGTTC ATGATGATAT GGAATGTTCA 
CATTACATGA AAAATTTTGA TGTTGGACAT GTGCCAATAA GGCTTCCAAG AACAAAACAC 
TTGTTAAATG TCATCAATGA AAACTTTGGA ACCCTTGCCT TCTGCCGCAG ATGGCTGGAT 
CGCTTGGGAG AAAGTAAATA CTTGATGGCT CTGAAGAATC TGTGTGACTT GGGCATTGTA 
GATCCATATC CACCATTATG TGACATTAAA GGATCATATA CAGCGCAATT TGAACATACC 
ATCCTGTTGC GTCCAACATG TAAAGAAGTT GTCAGCAGAG GAGATGACTA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001317175.1
CDS	135..1568
Translation	MAGVEEVAASGSHLNGDLDPDDREEGAASTAEEAAKKKRRKKKKSKGPSAGEQEPDKESGASVDEVARQLERSALEDKERDEDDEDGDGDGDGATGKKKKKKKKKRGPKVQTDPPSVPICDLYPNGVFPKGQECEYPPTQDGRTAAWRTTSEEKKALDQASEEIWNDFREAAEAHRQVRKYVMSWIKPGMTMIEICEKLEDCSRKLIKENGLNAGLAFPTGCSLNNCAAHYTPNAGDTTVLQYDDICKIDFGTHISGRIIDCAFTVTFNPKYDTLLKAVKDATNTGIKCAGIDVRLCDVGEAIQEVMESYEVEIDGKTYQVKPIRNLNGHSIGQYRIHAGKTVPIVKGGEATRMEEGEVYAIETFGSTGKGVVHDDMECSHYMKNFDVGHVPIRLPRTKHLLNVINENFGTLAFCRRWLDRLGESKYLMALKNLCDLGIVDPYPPLCDIKGSYTAQFEHTILLRPTCKEVVSRGDDY

Target ORF information:

RefSeq Version	NM_001330246.1
Organism	Homo sapiens(human)
Definition	Homo sapiens methionyl aminopeptidase 2 (METAP2), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001330246.1

ORF Insert Sequence:

ATGGCGGGTG TGGAGGAGGT AGCGGCCTCC GGGAGCCACC TGAATGGCGA CCTGGATCCA 
GACGACAGGG AAGAAGGAGC TGCCTCTACG GCTGAGGAAG CAGCCAAGAA AAAAAGACGA 
AAGAAGAAGA AGAGCAAAGG GCCTTCTGCA GGGGAACAGG AACCTGATAA AGAATCAGGA 
GCCTCAGTGG ATGAAGTAGC AAGACAGTTG GAAAGATCAG CATTGGAAGA TAAAGAAAGA 
GATGAAGATG ATGAAGATGG AGATGGCGAT GGAGATGGAG CAACTGGAAA GAAGAAGAAA 
AAGAAGAAGA AGAAGAGAGG ACCAAAAGTT CAAACAGACC CTCCCTCAGT TCCAATATGT 
GACCTGTATC CTAATGGTGT ATTTCCCAAA GGACAAGAAT GCGAATACCC ACCCACACAA 
GATGGGCGAA CAGCTGCTTG GAGAACTACA AGTGAAGAAA AGAAAGCATT AGATCAGGCA 
AGTGAAGAGA TTTGGAATGA TTTTCGAGAA GCTGCAGAAG CACATCGACA AGTTAGAAAA 
TACGTAATGA GCTGGATCAA GCCTGGGATG ACAATGATAG AAATCTGTGA AAAGTTGGAA 
GACTGTTCAC GCAAGTTAAT AAAAGAGAAT GGATTAAATG CAGGCCTGGC ATTTCCTACT 
GGATGTTCTC TCAATAATTG TGCTGCCCAT TATACTCCCA ATGCCGGTGA CACAACAGTA 
TTACAGTATG ATGACATCTG TAAAATAGAC TTTGGAACAC ATATAAGTGG TAGGATTATT 
GACTGTGCTT TTACTGTCAC TTTTAATCCC AAATATGATA CGTTATTAAA AGCTGTAAAA 
GATGCTACTA ACACTGGAAT AAAGTGTGCT GGAATTGATG TTCGTCTGTG TGATGTTGGT 
GAGGCCATCC AAGAAGTTAT GGAGTCCTAT GAAGTTGAAA TAGATGGGAA GACATATCAA 
GTGAAACCAA TCCGTAATCT AAATGGACAT TCAATTGGGC AATATAGAAT ACATGCTGGA 
AAAACAGTGC CGATTGTGAA AGGAGGGGAG GCAACAAGAA TGGAGGAAGG AGAAGTATAT 
GCAATTGAAA CCTTTGGTAG TACAGGAAAA GGTGTTGTTC ATGATGATAT GGAATGTTCA 
CATTACATGA AAAATTTTGA TGTTGGACAT GTGCCAATAA GGCTTCCAAG AACAAAACAC 
TTGTTAAATG TCATCAATGA AAACTTTGGA ACCCTTGCCT TCTGCCGCAG ATGGCTGGAT 
CGCTTGGGAG AAAGTAAATA CTTGATGGCT CTGAAGAATC TGTGTGACTT GGGCATTGTA 
GATCCATATC CACCATTATG TGACATTAAA GGATCATATA CAGCGCAATT TGAACATACC 
ATCCTGTTGC GTCCAACATG TAAAGAAGTT GTCAGCAGAG GAGATGACTA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

METAP2 ( NM_001330246.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001317175.1 Homo sapiens methionyl aminopeptidase 2 (METAP2), transcript variant 5, mRNA.

Price & Availability↑

CloneID	OHu46263
Clone ID Related Accession (Same CDS sequence)	NM_001330246.1 , NM_001330246.2
Accession Version	NM_001330246.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1434bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-06
Organism	Homo sapiens(human)
Product	methionine aminopeptidase 2 isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U13261.1, AK091730.1, AC018475.32 and CA450598.1. On May 31, 2019 this sequence version replaced NM_001330246.1. Transcript Variant: This variant (5) uses an alternate in-frame splice junction in the 5' coding region compared to variant 1. The resulting isoform (4) has the same N- and C-termini but is one amino acid shorter compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.205314.1, SRR1803615.114090.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGGTG TGGAGGAGGT AGCGGCCTCC GGGAGCCACC TGAATGGCGA CCTGGATCCA 
GACGACAGGG AAGAAGGAGC TGCCTCTACG GCTGAGGAAG CAGCCAAGAA AAAAAGACGA 
AAGAAGAAGA AGAGCAAAGG GCCTTCTGCA GGGGAACAGG AACCTGATAA AGAATCAGGA 
GCCTCAGTGG ATGAAGTAGC AAGACAGTTG GAAAGATCAG CATTGGAAGA TAAAGAAAGA 
GATGAAGATG ATGAAGATGG AGATGGCGAT GGAGATGGAG CAACTGGAAA GAAGAAGAAA 
AAGAAGAAGA AGAAGAGAGG ACCAAAAGTT CAAACAGACC CTCCCTCAGT TCCAATATGT 
GACCTGTATC CTAATGGTGT ATTTCCCAAA GGACAAGAAT GCGAATACCC ACCCACACAA 
GATGGGCGAA CAGCTGCTTG GAGAACTACA AGTGAAGAAA AGAAAGCATT AGATCAGGCA 
AGTGAAGAGA TTTGGAATGA TTTTCGAGAA GCTGCAGAAG CACATCGACA AGTTAGAAAA 
TACGTAATGA GCTGGATCAA GCCTGGGATG ACAATGATAG AAATCTGTGA AAAGTTGGAA 
GACTGTTCAC GCAAGTTAAT AAAAGAGAAT GGATTAAATG CAGGCCTGGC ATTTCCTACT 
GGATGTTCTC TCAATAATTG TGCTGCCCAT TATACTCCCA ATGCCGGTGA CACAACAGTA 
TTACAGTATG ATGACATCTG TAAAATAGAC TTTGGAACAC ATATAAGTGG TAGGATTATT 
GACTGTGCTT TTACTGTCAC TTTTAATCCC AAATATGATA CGTTATTAAA AGCTGTAAAA 
GATGCTACTA ACACTGGAAT AAAGTGTGCT GGAATTGATG TTCGTCTGTG TGATGTTGGT 
GAGGCCATCC AAGAAGTTAT GGAGTCCTAT GAAGTTGAAA TAGATGGGAA GACATATCAA 
GTGAAACCAA TCCGTAATCT AAATGGACAT TCAATTGGGC AATATAGAAT ACATGCTGGA 
AAAACAGTGC CGATTGTGAA AGGAGGGGAG GCAACAAGAA TGGAGGAAGG AGAAGTATAT 
GCAATTGAAA CCTTTGGTAG TACAGGAAAA GGTGTTGTTC ATGATGATAT GGAATGTTCA 
CATTACATGA AAAATTTTGA TGTTGGACAT GTGCCAATAA GGCTTCCAAG AACAAAACAC 
TTGTTAAATG TCATCAATGA AAACTTTGGA ACCCTTGCCT TCTGCCGCAG ATGGCTGGAT 
CGCTTGGGAG AAAGTAAATA CTTGATGGCT CTGAAGAATC TGTGTGACTT GGGCATTGTA 
GATCCATATC CACCATTATG TGACATTAAA GGATCATATA CAGCGCAATT TGAACATACC 
ATCCTGTTGC GTCCAACATG TAAAGAAGTT GTCAGCAGAG GAGATGACTA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001317175.1
CDS	29..1462
Translation	MAGVEEVAASGSHLNGDLDPDDREEGAASTAEEAAKKKRRKKKKSKGPSAGEQEPDKESGASVDEVARQLERSALEDKERDEDDEDGDGDGDGATGKKKKKKKKKRGPKVQTDPPSVPICDLYPNGVFPKGQECEYPPTQDGRTAAWRTTSEEKKALDQASEEIWNDFREAAEAHRQVRKYVMSWIKPGMTMIEICEKLEDCSRKLIKENGLNAGLAFPTGCSLNNCAAHYTPNAGDTTVLQYDDICKIDFGTHISGRIIDCAFTVTFNPKYDTLLKAVKDATNTGIKCAGIDVRLCDVGEAIQEVMESYEVEIDGKTYQVKPIRNLNGHSIGQYRIHAGKTVPIVKGGEATRMEEGEVYAIETFGSTGKGVVHDDMECSHYMKNFDVGHVPIRLPRTKHLLNVINENFGTLAFCRRWLDRLGESKYLMALKNLCDLGIVDPYPPLCDIKGSYTAQFEHTILLRPTCKEVVSRGDDY

Target ORF information:

RefSeq Version	NM_001330246.2
Organism	Homo sapiens(human)
Definition	Homo sapiens methionyl aminopeptidase 2 (METAP2), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001330246.2

ORF Insert Sequence:

ATGGCGGGTG TGGAGGAGGT AGCGGCCTCC GGGAGCCACC TGAATGGCGA CCTGGATCCA 
GACGACAGGG AAGAAGGAGC TGCCTCTACG GCTGAGGAAG CAGCCAAGAA AAAAAGACGA 
AAGAAGAAGA AGAGCAAAGG GCCTTCTGCA GGGGAACAGG AACCTGATAA AGAATCAGGA 
GCCTCAGTGG ATGAAGTAGC AAGACAGTTG GAAAGATCAG CATTGGAAGA TAAAGAAAGA 
GATGAAGATG ATGAAGATGG AGATGGCGAT GGAGATGGAG CAACTGGAAA GAAGAAGAAA 
AAGAAGAAGA AGAAGAGAGG ACCAAAAGTT CAAACAGACC CTCCCTCAGT TCCAATATGT 
GACCTGTATC CTAATGGTGT ATTTCCCAAA GGACAAGAAT GCGAATACCC ACCCACACAA 
GATGGGCGAA CAGCTGCTTG GAGAACTACA AGTGAAGAAA AGAAAGCATT AGATCAGGCA 
AGTGAAGAGA TTTGGAATGA TTTTCGAGAA GCTGCAGAAG CACATCGACA AGTTAGAAAA 
TACGTAATGA GCTGGATCAA GCCTGGGATG ACAATGATAG AAATCTGTGA AAAGTTGGAA 
GACTGTTCAC GCAAGTTAAT AAAAGAGAAT GGATTAAATG CAGGCCTGGC ATTTCCTACT 
GGATGTTCTC TCAATAATTG TGCTGCCCAT TATACTCCCA ATGCCGGTGA CACAACAGTA 
TTACAGTATG ATGACATCTG TAAAATAGAC TTTGGAACAC ATATAAGTGG TAGGATTATT 
GACTGTGCTT TTACTGTCAC TTTTAATCCC AAATATGATA CGTTATTAAA AGCTGTAAAA 
GATGCTACTA ACACTGGAAT AAAGTGTGCT GGAATTGATG TTCGTCTGTG TGATGTTGGT 
GAGGCCATCC AAGAAGTTAT GGAGTCCTAT GAAGTTGAAA TAGATGGGAA GACATATCAA 
GTGAAACCAA TCCGTAATCT AAATGGACAT TCAATTGGGC AATATAGAAT ACATGCTGGA 
AAAACAGTGC CGATTGTGAA AGGAGGGGAG GCAACAAGAA TGGAGGAAGG AGAAGTATAT 
GCAATTGAAA CCTTTGGTAG TACAGGAAAA GGTGTTGTTC ATGATGATAT GGAATGTTCA 
CATTACATGA AAAATTTTGA TGTTGGACAT GTGCCAATAA GGCTTCCAAG AACAAAACAC 
TTGTTAAATG TCATCAATGA AAACTTTGGA ACCCTTGCCT TCTGCCGCAG ATGGCTGGAT 
CGCTTGGGAG AAAGTAAATA CTTGATGGCT CTGAAGAATC TGTGTGACTT GGGCATTGTA 
GATCCATATC CACCATTATG TGACATTAAA GGATCATATA CAGCGCAATT TGAACATACC 
ATCCTGTTGC GTCCAACATG TAAAGAAGTT GTCAGCAGAG GAGATGACTA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Suppression of glioblastoma growth and angiogenesis through molecular targeting of methionine aminopeptidase-2.
Journal of neuro-oncology136(2)243-254(2018 Jan)
Lin M,Zhang X,Jia B,Guan S

MetAP1 and MetAP2 drive cell selectivity for a potent anti-cancer agent in synergy, by controlling glutathione redox state.
Oncotarget7(39)63306-63323(2016 Sep)
Frottin F,Bienvenut WV,Bignon J,Jacquet E,Vaca Jacome AS,Van Dorsselaer A,Cianferani S,Carapito C,Meinnel T,Giglione C

Methionine Aminopeptidase 2 as a Potential Therapeutic Target for Human Non-Small-Cell Lung Cancers.
Advances in clinical and experimental medicine : official organ Wroclaw Medical University25(1)117-28(2016 Jan-Feb)
Shimizu H,Yamagishi S,Chiba H,Ghazizadeh M

N-terminal acetylome analysis reveals the specificity of Naa50 (Nat5) and suggests a kinetic competition between N-terminal acetyltransferases and methionine aminopeptidases.
Proteomics15(14)2436-46(2015 Jul)
Van Damme P,Hole K,Gevaert K,Arnesen T

Redox regulation of methionine aminopeptidase 2 activity.
The Journal of biological chemistry289(21)15035-43(2014 May)
Chiu J,Wong JW,Hogg PJ

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METAP2 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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