TMED10 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	TMED10
Entrez Gene ID	10972
Full Name	transmembrane p24 trafficking protein 10
Synonyms	P24(DELTA),S31I125,S31III125,TMP21,Tmp-21-I,p23,p24d1
General protein information	Preferred Names transmembrane p24 trafficking protein 10 Names transmembrane emp24 domain-containing protein 10 21 kDa transmembrane trafficking protein p24 family protein delta-1 p24delta p24delta1 testicular tissue protein Li 206 transmembrane emp24-like trafficking protein 10 transmembrane protein Tmp21
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 14 Map Location: 14q24.3
Summary	This gene is a member of the EMP24/GP25L/p24 family and encodes a protein with a GOLD domain. This type I membrane protein is localized to the plasma membrane and golgi cisternae and is involved in vesicular protein trafficking. The protein is also a member of a heteromeric secretase complex and regulates the complex's gamma-secretase activity without affecting its epsilon-secretase activity. Mutations in this gene have been associated with early-onset familial Alzheimer's disease. This gene has a pseudogene on chromosome 8. [provided by RefSeq, Jul 2008].
Disorder MIM:	605406

mRNA and Protein(s)

mRNA	Protein	Name
NM_006827.5	NP_006818.3	transmembrane emp24 domain-containing protein 10 precursor
NM_006827.6	NP_006818.3	transmembrane emp24 domain-containing protein 10 precursor

Pathways from BioSystems

Homologies

	LOC701522	XP_001089815.2
Mus musculus (house mouse)	Gm4024	XP_001478849.1
Gallus gallus (chicken)	TMED10	NP_001006482.1
Danio rerio (zebrafish)	tmed10	NP_999863.1
Drosophila melanogaster (fruit fly)	bai	NP_651323.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001064	XP_318301.4
Neurospora crassa	NCU01342	XP_961428.2
Arabidopsis thaliana (thale cress)	AT1G57620	NP_176075.1
Homo sapiens (human)	TMED10	NP_006818.3
Pan troglodytes (chimpanzee)	TMED10	XP_510074.3
Kluyveromyces lactis	KLLA0B00935g	XP_451572.1
Schizosaccharomyces pombe (fission yeast)	erv25	NP_593403.1
Mus musculus (house mouse)	Tmed10	NP_081051.1
Rattus norvegicus (Norway rat)	Tmed10	NP_445919.1
Saccharomyces cerevisiae (baker's yeast)	ERV25	NP_013701.1
Arabidopsis thaliana (thale cress)	AT1G21900	NP_173608.1
Bos taurus (cattle)	TMED10	NP_001035639.1
Canis lupus familiaris (dog)	TMED10	XP_854043.1
Caenorhabditis elegans (roundworm)	F47G9.1	NP_505879.1
Magnaporthe oryzae (rice blast fungus)	MGG_08210	XP_003715192.1
Arabidopsis thaliana (thale cress)	AT1G09580	NP_172429.1
Oryza sativa (rice)	Os02g0768200	NP_001048237.1
Oryza sativa (rice)	Os06g0210100	NP_001057112.2
Xenopus tropicalis (tropical clawed frog)	LOC549012	NP_001016258.1

Gene Ontology
Bibliography

Related articles in PubMed

TMED10 Protein Interferes with Transforming Growth Factor (TGF)-? Signaling by Disrupting TGF-? Receptor Complex Formation.
Nakano N, Tsuchiya Y, Kako K, Umezaki K, Sano K, Ikeno S, Otsuka E, Shigeta M, Nakagawa A, Sakata N, Itoh F, Nakano Y, Iemura SI, van Dinther M, Natsume T, Ten Dijke P, Itoh S
The Journal of biological chemistry292(10)4099-4112(2017 03)

A Novel Alzheimer-Associated SNP in Tmp21 Increases Amyloidogenesis.
Zhang X, Wu Y, Cai F, Liu S, Bromley-Brits K, Xia K, Song W
Molecular neurobiology(2017 Feb)

3D Structure and Interaction of p24? and p24? Golgi Dynamics Domains: Implication for p24 Complex Formation and Cargo Transport.
Nagae M, Hirata T, Morita-Matsumoto K, Theiler R, Fujita M, Kinoshita T, Yamaguchi Y
Journal of molecular biology428(20)4087-4099(2016 Oct)

Quantitative interaction proteomics of neurodegenerative disease proteins.
Hosp F, Vossfeldt H, Heinig M, Vasiljevic D, Arumughan A, Wyler E, , Landthaler M, Hubner N, Wanker EE, Lannfelt L, Ingelsson M, Lalowski M, Voigt A, Selbach M
Cell reports11(7)1134-46(2015 May)

TMP21 modulates cell growth in papillary thyroid cancer cells by inducing autophagy through activation of the AMPK/mTOR pathway.
Xu X, Gao H, Qin J, He L, Liu W
International journal of clinical and experimental pathology8(9)10824-31(2015)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Low TMED10 expression is associated with breast cancer.
Title: TMED10 Protein Interferes with Transforming Growth Factor (TGF)-? Signaling by Disrupting TGF-? Receptor Complex Formation.

Surface plasmon resonance and solution NMR analyses revealed that p24beta1 and p24delta1 GOLD domains interact weakly (Kd= ~10(-4)M).
Title: 3D Structure and Interaction of p24? and p24? Golgi Dynamics Domains: Implication for p24 Complex Formation and Cargo Transport.

TMP21 modulates cell growth in papillary thyroid cancer cells by inducing autophagy, which may be associated with activation of AMPK/mTOR pathway.
Title: TMP21 modulates cell growth in papillary thyroid cancer cells by inducing autophagy through activation of the AMPK/mTOR pathway.

Study demonstrates that NleF binds to the human Tmp21 protein and subsequently disrupts intracellular protein trafficking.
Title: The enterohemorrhagic Escherichia coli effector protein NleF binds mammalian Tmp21.

This study suggested that The role ofTMP21 in the modulation of gamma-secretase activity and protein trafficking and related to alzheimer disease.
Title: The role of TMP21 in trafficking and amyloid-? precursor protein (APP) processing in Alzheimer's disease.

The following TMED10 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TMED10 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu05787	NM_006827.6 Latest version!	Homo sapiens transmembrane p24 trafficking protein 10 (TMED10), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu05787	NM_006827.5	Homo sapiens transmembrane p24 trafficking protein 10 (TMED10), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TMED10 ( NM_006827.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_006818.3 Homo sapiens transmembrane p24 trafficking protein 10 (TMED10), mRNA.

Price & Availability↑

CloneID	OHu05787
Clone ID Related Accession (Same CDS sequence)	NM_006827.5 , NM_006827.6
Accession Version	NM_006827.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 660bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	transmembrane emp24 domain-containing protein 10 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX504393.1, X97442.1, BX248754.1, AK056156.1, BG675810.1, CN420777.1, AL832012.2 and DB528262.1. On May 19, 2006 this sequence version replaced NM_006827.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: HM005519.1, SRR1660807.159260.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCTGGTT TGTCTGGCCC ACCAGCCCGG CGCGGCCCTT TTCCGTTAGC GTTGCTGCTT 
TTGTTCCTGC TCGGCCCCAG ATTGGTCCTT GCCATCTCCT TCCATCTGCC CATTAACTCT 
CGCAAGTGCC TCCGTGAGGA GATTCACAAG GACCTGCTAG TGACTGGCGC GTACGAGATC 
TCCGACCAGT CTGGGGGCGC TGGCGGCCTG CGCAGCCACC TCAAGATCAC AGATTCTGCT 
GGCCATATTC TCTACTCCAA AGAGGATGCA ACCAAGGGGA AATTTGCCTT TACCACTGAA 
GATTATGACA TGTTTGAAGT GTGTTTTGAG AGCAAGGGAA CAGGGCGGAT ACCTGACCAA 
CTCGTGATCC TAGACATGAA GCATGGAGTG GAGGCGAAAA ATTACGAAGA GATTGCAAAA 
GTTGAGAAGC TCAAACCATT AGAGGTAGAG CTGCGACGCC TAGAAGACCT TTCAGAATCT 
ATTGTTAATG ATTTTGCCTA CATGAAGAAG AGAGAAGAGG AGATGCGTGA TACCAACGAG 
TCAACAAACA CTCGGGTCCT ATACTTCAGC ATCTTTTCAA TGTTCTGTCT CATTGGACTA 
GCTACCTGGC AGGTCTTCTA CCTGCGACGC TTCTTCAAGG CCAAGAAATT GATTGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_006818.3
CDS	68..727
Translation	MSGLSGPPARRGPFPLALLLLFLLGPRLVLAISFHLPINSRKCLREEIHKDLLVTGAYEISDQSGGAGGLRSHLKITDSAGHILYSKEDATKGKFAFTTEDYDMFEVCFESKGTGRIPDQLVILDMKHGVEAKNYEEIAKVEKLKPLEVELRRLEDLSESIVNDFAYMKKREEEMRDTNESTNTRVLYFSIFSMFCLIGLATWQVFYLRRFFKAKKLIE

Target ORF information:

RefSeq Version	NM_006827.5
Organism	Homo sapiens(human)
Definition	Homo sapiens transmembrane p24 trafficking protein 10 (TMED10), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006827.5

ORF Insert Sequence:

ATGTCTGGTT TGTCTGGCCC ACCAGCCCGG CGCGGCCCTT TTCCGTTAGC GTTGCTGCTT 
TTGTTCCTGC TCGGCCCCAG ATTGGTCCTT GCCATCTCCT TCCATCTGCC CATTAACTCT 
CGCAAGTGCC TCCGTGAGGA GATTCACAAG GACCTGCTAG TGACTGGCGC GTACGAGATC 
TCCGACCAGT CTGGGGGCGC TGGCGGCCTG CGCAGCCACC TCAAGATCAC AGATTCTGCT 
GGCCATATTC TCTACTCCAA AGAGGATGCA ACCAAGGGGA AATTTGCCTT TACCACTGAA 
GATTATGACA TGTTTGAAGT GTGTTTTGAG AGCAAGGGAA CAGGGCGGAT ACCTGACCAA 
CTCGTGATCC TAGACATGAA GCATGGAGTG GAGGCGAAAA ATTACGAAGA GATTGCAAAA 
GTTGAGAAGC TCAAACCATT AGAGGTAGAG CTGCGACGCC TAGAAGACCT TTCAGAATCT 
ATTGTTAATG ATTTTGCCTA CATGAAGAAG AGAGAAGAGG AGATGCGTGA TACCAACGAG 
TCAACAAACA CTCGGGTCCT ATACTTCAGC ATCTTTTCAA TGTTCTGTCT CATTGGACTA 
GCTACCTGGC AGGTCTTCTA CCTGCGACGC TTCTTCAAGG CCAAGAAATT GATTGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TMED10 ( NM_006827.6 ) cDNA ORF clone, Homo sapiens(human) -> NP_006818.3 Homo sapiens transmembrane p24 trafficking protein 10 (TMED10), mRNA.

Price & Availability↑

CloneID	OHu05787
Clone ID Related Accession (Same CDS sequence)	NM_006827.5 , NM_006827.6
Accession Version	NM_006827.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 660bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-16
Organism	Homo sapiens(human)
Product	transmembrane emp24 domain-containing protein 10 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX504393.1, X97442.1, BX248754.1, AK056156.1, BG675810.1, CN420777.1, AL832012.2 and DB528262.1. On Nov 22, 2018 this sequence version replaced NM_006827.5. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.111188.1, SRR1660803.120772.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000303575.9/ ENSP00000303145.4 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTCTGGTT TGTCTGGCCC ACCAGCCCGG CGCGGCCCTT TTCCGTTAGC GTTGCTGCTT 
TTGTTCCTGC TCGGCCCCAG ATTGGTCCTT GCCATCTCCT TCCATCTGCC CATTAACTCT 
CGCAAGTGCC TCCGTGAGGA GATTCACAAG GACCTGCTAG TGACTGGCGC GTACGAGATC 
TCCGACCAGT CTGGGGGCGC TGGCGGCCTG CGCAGCCACC TCAAGATCAC AGATTCTGCT 
GGCCATATTC TCTACTCCAA AGAGGATGCA ACCAAGGGGA AATTTGCCTT TACCACTGAA 
GATTATGACA TGTTTGAAGT GTGTTTTGAG AGCAAGGGAA CAGGGCGGAT ACCTGACCAA 
CTCGTGATCC TAGACATGAA GCATGGAGTG GAGGCGAAAA ATTACGAAGA GATTGCAAAA 
GTTGAGAAGC TCAAACCATT AGAGGTAGAG CTGCGACGCC TAGAAGACCT TTCAGAATCT 
ATTGTTAATG ATTTTGCCTA CATGAAGAAG AGAGAAGAGG AGATGCGTGA TACCAACGAG 
TCAACAAACA CTCGGGTCCT ATACTTCAGC ATCTTTTCAA TGTTCTGTCT CATTGGACTA 
GCTACCTGGC AGGTCTTCTA CCTGCGACGC TTCTTCAAGG CCAAGAAATT GATTGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_006818.3
CDS	34..693
Translation	MSGLSGPPARRGPFPLALLLLFLLGPRLVLAISFHLPINSRKCLREEIHKDLLVTGAYEISDQSGGAGGLRSHLKITDSAGHILYSKEDATKGKFAFTTEDYDMFEVCFESKGTGRIPDQLVILDMKHGVEAKNYEEIAKVEKLKPLEVELRRLEDLSESIVNDFAYMKKREEEMRDTNESTNTRVLYFSIFSMFCLIGLATWQVFYLRRFFKAKKLIE

Target ORF information:

RefSeq Version	NM_006827.6
Organism	Homo sapiens(human)
Definition	Homo sapiens transmembrane p24 trafficking protein 10 (TMED10), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006827.6

ORF Insert Sequence:

ATGTCTGGTT TGTCTGGCCC ACCAGCCCGG CGCGGCCCTT TTCCGTTAGC GTTGCTGCTT 
TTGTTCCTGC TCGGCCCCAG ATTGGTCCTT GCCATCTCCT TCCATCTGCC CATTAACTCT 
CGCAAGTGCC TCCGTGAGGA GATTCACAAG GACCTGCTAG TGACTGGCGC GTACGAGATC 
TCCGACCAGT CTGGGGGCGC TGGCGGCCTG CGCAGCCACC TCAAGATCAC AGATTCTGCT 
GGCCATATTC TCTACTCCAA AGAGGATGCA ACCAAGGGGA AATTTGCCTT TACCACTGAA 
GATTATGACA TGTTTGAAGT GTGTTTTGAG AGCAAGGGAA CAGGGCGGAT ACCTGACCAA 
CTCGTGATCC TAGACATGAA GCATGGAGTG GAGGCGAAAA ATTACGAAGA GATTGCAAAA 
GTTGAGAAGC TCAAACCATT AGAGGTAGAG CTGCGACGCC TAGAAGACCT TTCAGAATCT 
ATTGTTAATG ATTTTGCCTA CATGAAGAAG AGAGAAGAGG AGATGCGTGA TACCAACGAG 
TCAACAAACA CTCGGGTCCT ATACTTCAGC ATCTTTTCAA TGTTCTGTCT CATTGGACTA 
GCTACCTGGC AGGTCTTCTA CCTGCGACGC TTCTTCAAGG CCAAGAAATT GATTGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

TMED10 Protein Interferes with Transforming Growth Factor (TGF)-? Signaling by Disrupting TGF-? Receptor Complex Formation.
The Journal of biological chemistry292(10)4099-4112(2017 03)
Nakano N,Tsuchiya Y,Kako K,Umezaki K,Sano K,Ikeno S,Otsuka E,Shigeta M,Nakagawa A,Sakata N,Itoh F,Nakano Y,Iemura SI,van Dinther M,Natsume T,Ten Dijke P,Itoh S

A Novel Alzheimer-Associated SNP in Tmp21 Increases Amyloidogenesis.
Molecular neurobiology(2017 Feb)
Zhang X,Wu Y,Cai F,Liu S,Bromley-Brits K,Xia K,Song W

3D Structure and Interaction of p24? and p24? Golgi Dynamics Domains: Implication for p24 Complex Formation and Cargo Transport.
Journal of molecular biology428(20)4087-4099(2016 Oct)
Nagae M,Hirata T,Morita-Matsumoto K,Theiler R,Fujita M,Kinoshita T,Yamaguchi Y

Quantitative interaction proteomics of neurodegenerative disease proteins.
Cell reports11(7)1134-46(2015 May)
Hosp F,Vossfeldt H,Heinig M,Vasiljevic D,Arumughan A,Wyler E,,Landthaler M,Hubner N,Wanker EE,Lannfelt L,Ingelsson M,Lalowski M,Voigt A,Selbach M

TMP21 modulates cell growth in papillary thyroid cancer cells by inducing autophagy through activation of the AMPK/mTOR pathway.
International journal of clinical and experimental pathology8(9)10824-31(2015)
Xu X,Gao H,Qin J,He L,Liu W

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TMED10 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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