SEC61B cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	SEC61B
Entrez Gene ID	10952
Full Name	Sec61 translocon beta subunit
General protein information	Preferred Names Sec61 translocon beta subunit Names protein transport protein Sec61 subunit beta Sec61 beta subunit Sec61 complex, beta subunit protein translocation complex beta protein transport protein SEC61 beta subunit
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 9 Map Location: 9q22.33
Summary	The Sec61 complex is the central component of the protein translocation apparatus of the endoplasmic reticulum (ER) membrane. Oligomers of the Sec61 complex form a transmembrane channel where proteins are translocated across and integrated into the ER membrane. This complex consists of three membrane proteins- alpha, beta, and gamma. This gene encodes the beta-subunit protein. The Sec61 subunits are also observed in the post-ER compartment, suggesting that these proteins can escape the ER and recycle back. There is evidence for multiple polyadenylated sites for this transcript. [provided by RefSeq, Jul 2008].
Disorder MIM:	609214

mRNA and Protein(s)

mRNA	Protein	Name
NM_006808.2	NP_006799.1	protein transport protein Sec61 subunit beta
NM_006808.3	NP_006799.1	protein transport protein Sec61 subunit beta

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	Sec61beta	NP_652037.1
Gallus gallus (chicken)	SEC61B	XP_419082.3
Homo sapiens (human)	SEC61B	NP_006799.1
Rattus norvegicus (Norway rat)	Sec61b	NP_001100124.1
Xenopus tropicalis (tropical clawed frog)	sec61b	NP_001103514.1
Canis lupus familiaris (dog)	SEC61B	NP_001003326.1
Bos taurus (cattle)	SEC61B	NP_001068760.1
Mus musculus (house mouse)	Sec61b	NP_077133.1
Danio rerio (zebrafish)	sec61b	NP_001002527.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011504	XP_317795.4
Pan troglodytes (chimpanzee)	SEC61B	XP_001157550.2
	SEC61B	XP_001109722.1

Gene Ontology
Bibliography

Related articles in PubMed

Sec61? facilitates the maintenance of endoplasmic reticulum homeostasis by associating microtubules.
Zhu Y, Zhang G, Lin S, Shi J, Zhang H, Hu J
Protein & cell(2017 Nov)

Isolated polycystic liver disease genes define effectors of polycystin-1 function.
Besse W, Dong K, Choi J, Punia S, Fedeles SV, Choi M, Gallagher AR, Huang EB, Gulati A, Knight J, Mane S, Tahvanainen E, Tahvanainen P, Sanna-Cherchi S, Lifton RP, Watnick T, Pei YP, Torres VE, Somlo S
The Journal of clinical investigation127(5)1772-1785(2017 May)

Sec61? regulates barrier functions of tight junction through expression of claudin-4 in Madin-Darby canine kidney cells.
Washiyama M, Koizumi N, Fujii M, Watanabe Y
Biological & pharmaceutical bulletin37(6)979-86(2014)

Genetics of callous-unemotional behavior in children.
Viding E, Price TS, Jaffee SR, Trzaskowski M, Davis OS, Meaburn EL, Haworth CM, Plomin R
PloS one8(7)e65789(2013)

Exocyst Sec10 is involved in basolateral protein translation and translocation in the endoplasmic reticulum.
Choi SY, Fogelgren B, Zuo X, Huang L, McKenna S, Lingappa VR, Lipschutz JH
Nephron. Experimental nephrology120(4)e134-40(2012)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

used whole exome sequencing in a discovery cohort of 102 unrelated patients who were excluded for mutations in the 2 most common polycystic liver disease genes, PRKCSH and SEC63, to identify heterozygous loss-of-function mutations in 3 additional genes, ALG8, GANAB, and SEC61B. Similarly to PRKCSH and SEC63, these genes encode proteins that are integral to the protein biogenesis pathway in the endoplasmic reticulum.
Title: Isolated polycystic liver disease genes define effectors of polycystin-1 function.

Sec61beta overexpression increased tight junction modulation rates, in conjunction with enhanced delivery of claudin-4 from and to plasma membranes.
Title: Sec61? regulates barrier functions of tight junction through expression of claudin-4 in Madin-Darby canine kidney cells.

Sec61beta-KD cells also exhibited altered ATP7A cellular distribution.
Title: Sec61? controls sensitivity to platinum-containing chemotherapeutic agents through modulation of the copper-transporting ATPase ATP7A.

These data confirm that the exocyst is preferentially involved in basolateral protein translation and translocation, and may well act through the phosphorylation of Sec61beta.
Title: Exocyst Sec10 is involved in basolateral protein translation and translocation in the endoplasmic reticulum.

SEC61beta and its autoantibody as biomarkers for colorectal cancer
Title: Identification of SEC61? and its autoantibody as biomarkers for colorectal cancer.

The following SEC61B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SEC61B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu13168	NM_006808.3 Latest version!	Homo sapiens SEC61 translocon subunit beta (SEC61B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu13168	NM_006808.2	Homo sapiens Sec61 translocon beta subunit (SEC61B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SEC61B ( NM_006808.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_006799.1 Homo sapiens Sec61 translocon beta subunit (SEC61B), mRNA.

Price & Availability↑

CloneID	OHu13168
Clone ID Related Accession (Same CDS sequence)	NM_006808.2 , NM_006808.3
Accession Version	NM_006808.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 291bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-17
Organism	Homo sapiens(human)
Product	protein transport protein Sec61 subunit beta
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC001734.1. On Jul 3, 2001 this sequence version replaced NM_006808.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC001734.1, AK223384.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCTGGTC CGACCCCCAG TGGCACTAAC GTGGGATCCT CAGGGCGCTC TCCCAGCAAA 
GCAGTGGCCG CCCGGGCGGC GGGATCCACT GTCCGGCAGA GGAAAAATGC CAGCTGTGGG 
ACAAGGAGTG CAGGCCGCAC AACCTCGGCA GGCACCGGGG GGATGTGGCG ATTCTACACA 
GAAGATTCAC CTGGGCTCAA AGTTGGCCCT GTTCCAGTAT TGGTTATGAG TCTTCTGTTC 
ATCGCTTCTG TATTTATGTT GCACATTTGG GGCAAGTACA CTCGTTCGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_006799.1
CDS	77..367
Translation	MPGPTPSGTNVGSSGRSPSKAVAARAAGSTVRQRKNASCGTRSAGRTTSAGTGGMWRFYTEDSPGLKVGPVPVLVMSLLFIASVFMLHIWGKYTRS

Target ORF information:

RefSeq Version	NM_006808.2
Organism	Homo sapiens(human)
Definition	Homo sapiens Sec61 translocon beta subunit (SEC61B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006808.2

ORF Insert Sequence:

ATGCCTGGTC CGACCCCCAG TGGCACTAAC GTGGGATCCT CAGGGCGCTC TCCCAGCAAA 
GCAGTGGCCG CCCGGGCGGC GGGATCCACT GTCCGGCAGA GGAAAAATGC CAGCTGTGGG 
ACAAGGAGTG CAGGCCGCAC AACCTCGGCA GGCACCGGGG GGATGTGGCG ATTCTACACA 
GAAGATTCAC CTGGGCTCAA AGTTGGCCCT GTTCCAGTAT TGGTTATGAG TCTTCTGTTC 
ATCGCTTCTG TATTTATGTT GCACATTTGG GGCAAGTACA CTCGTTCGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SEC61B ( NM_006808.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_006799.1 Homo sapiens SEC61 translocon subunit beta (SEC61B), mRNA.

Price & Availability↑

CloneID	OHu13168
Clone ID Related Accession (Same CDS sequence)	NM_006808.2 , NM_006808.3
Accession Version	NM_006808.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 291bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-26
Organism	Homo sapiens(human)
Product	protein transport protein Sec61 subunit beta
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL137067.7 and BC001734.1. On Nov 23, 2018 this sequence version replaced NM_006808.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: ERR279854.286.1, SRR1660807.58439.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000223641.5/ ENSP00000223641.4 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCCTGGTC CGACCCCCAG TGGCACTAAC GTGGGATCCT CAGGGCGCTC TCCCAGCAAA 
GCAGTGGCCG CCCGGGCGGC GGGATCCACT GTCCGGCAGA GGAAAAATGC CAGCTGTGGG 
ACAAGGAGTG CAGGCCGCAC AACCTCGGCA GGCACCGGGG GGATGTGGCG ATTCTACACA 
GAAGATTCAC CTGGGCTCAA AGTTGGCCCT GTTCCAGTAT TGGTTATGAG TCTTCTGTTC 
ATCGCTTCTG TATTTATGTT GCACATTTGG GGCAAGTACA CTCGTTCGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_006799.1
CDS	83..373
Translation	MPGPTPSGTNVGSSGRSPSKAVAARAAGSTVRQRKNASCGTRSAGRTTSAGTGGMWRFYTEDSPGLKVGPVPVLVMSLLFIASVFMLHIWGKYTRS

Target ORF information:

RefSeq Version	NM_006808.3
Organism	Homo sapiens(human)
Definition	Homo sapiens SEC61 translocon subunit beta (SEC61B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006808.3

ORF Insert Sequence:

ATGCCTGGTC CGACCCCCAG TGGCACTAAC GTGGGATCCT CAGGGCGCTC TCCCAGCAAA 
GCAGTGGCCG CCCGGGCGGC GGGATCCACT GTCCGGCAGA GGAAAAATGC CAGCTGTGGG 
ACAAGGAGTG CAGGCCGCAC AACCTCGGCA GGCACCGGGG GGATGTGGCG ATTCTACACA 
GAAGATTCAC CTGGGCTCAA AGTTGGCCCT GTTCCAGTAT TGGTTATGAG TCTTCTGTTC 
ATCGCTTCTG TATTTATGTT GCACATTTGG GGCAAGTACA CTCGTTCGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sec61? facilitates the maintenance of endoplasmic reticulum homeostasis by associating microtubules.
Protein & cell(2017 Nov)
Zhu Y,Zhang G,Lin S,Shi J,Zhang H,Hu J

Isolated polycystic liver disease genes define effectors of polycystin-1 function.
The Journal of clinical investigation127(5)1772-1785(2017 May)
Besse W,Dong K,Choi J,Punia S,Fedeles SV,Choi M,Gallagher AR,Huang EB,Gulati A,Knight J,Mane S,Tahvanainen E,Tahvanainen P,Sanna-Cherchi S,Lifton RP,Watnick T,Pei YP,Torres VE,Somlo S

Sec61? regulates barrier functions of tight junction through expression of claudin-4 in Madin-Darby canine kidney cells.
Biological & pharmaceutical bulletin37(6)979-86(2014)
Washiyama M,Koizumi N,Fujii M,Watanabe Y

Genetics of callous-unemotional behavior in children.
PloS one8(7)e65789(2013)
Viding E,Price TS,Jaffee SR,Trzaskowski M,Davis OS,Meaburn EL,Haworth CM,Plomin R

Exocyst Sec10 is involved in basolateral protein translation and translocation in the endoplasmic reticulum.
Nephron. Experimental nephrology120(4)e134-40(2012)
Choi SY,Fogelgren B,Zuo X,Huang L,McKenna S,Lingappa VR,Lipschutz JH

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SEC61B cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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