MAGED2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	MAGED2
Entrez Gene ID	10916
Full Name	MAGE family member D2
Synonyms	11B6,BARTS5,BCG-1,BCG1,HCA10,MAGE-D2
General protein information	Preferred Names MAGE family member D2 Names melanoma-associated antigen D2 MAGE-D2 antigen breast cancer-associated gene 1 protein hepatocellular carcinoma-associated protein JCL-1 melanoma antigen family D, 2 melanoma antigen family D2
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: X Map Location: Xp11.21
Summary	This gene is a member of the MAGED gene family. The MAGED genes are clustered on chromosome Xp11. This gene is located in Xp11.2, a hot spot for X-linked intellectual disability (XLID). Mutations in this gene cause a form of transient antenatal Bartter's syndrome. This gene may also be involved in several types of cancer, including breast cancer and melanoma. The protein encoded by this gene is progressively recruited from the cytoplasm to the nucleoplasm during the interphase and after nucleolar stress and is thus thought to play a role in cell cycle regulation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2017].
Disorder MIM:	300470
Disorder Html:	Bartter syndrome, type 5, antenatal, transient, 300971 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_177433.2	NP_803182.1	melanoma-associated antigen D2
NM_201222.3	NP_957516.1	melanoma-associated antigen D2
NM_014599.5	NP_055414.2	melanoma-associated antigen D2
NM_177433.3	NP_803182.1	melanoma-associated antigen D2
NM_014599.6	NP_055414.2	melanoma-associated antigen D2
NM_201222.2	NP_957516.1	melanoma-associated antigen D2

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Maged2	NP_001258038.1
Bos taurus (cattle)	MAGED2	NP_001069133.1
Canis lupus familiaris (dog)	MAGED2	XP_005641452.1
Pan troglodytes (chimpanzee)	MAGED2	XP_003317525.1
Mus musculus (house mouse)	Maged2	NP_109625.1
Homo sapiens (human)	MAGED2	NP_055414.2
	MAGED2	XP_001091068.1

Gene Ontology
Bibliography

Related articles in PubMed

Prevalence of Novel
Legrand A, Treard C, Roncelin I, Dreux S, Bertholet-Thomas A, Broux F, Bruno D, Decramer S, Deschenes G, Djeddi D, Guigonis V, Jay N, Khalifeh T, Llanas B, Morin D, Morin G, Nobili F, Pietrement C, Ryckewaert A, Salomon R, Vrillon I, Blanchard A, Vargas-Poussou R
Clinical journal of the American Society of Nephrology : CJASN(2017 Nov)

Polyhydramnios, Transient Antenatal Bartter's Syndrome, and MAGED2 Mutations.
Laghmani K, Beck BB, Yang SS, Seaayfan E, Wenzel A, Reusch B, Vitzthum H, Priem D, Demaretz S, Bergmann K, Duin LK, G?bel H, Mache C, Thiele H, Bartram MP, Dombret C, Altm?ller J, N?rnberg P, Benzing T, Levtchenko E, Seyberth HW, Klaus G, Yigit G, Lin SH, Timmer A, de Koning TJ, Scherjon SA, Schlingmann KP, Bertrand MJ, Rinschen MM, de Backer O, Konrad M, K?mhoff M
The New England journal of medicine374(19)1853-63(2016 May)

Melanoma antigen-D2: A nucleolar protein undergoing delocalization during cell cycle and after cellular stress.
Pirlot C, Thiry M, Trussart C, Di Valentin E, Piette J, Habraken Y
Biochimica et biophysica acta1863(4)581-95(2016 Apr)

Integrative analysis of kinase networks in TRAIL-induced apoptosis provides a source of potential targets for combination therapy.
So J, Pasculescu A, Dai AY, Williton K, James A, Nguyen V, Creixell P, Schoof EM, Sinclair J, Barrios-Rodiles M, Gu J, Krizus A, Williams R, Olhovsky M, Dennis JW, Wrana JL, Linding R, Jorgensen C, Pawson T, Colwill K
Science signaling8(371)rs3(2015 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

We found that MAGED2 mutations caused X-linked polyhydramnios with prematurity and a severe but transient form of antenatal Bartter's syndrome.
Title: Polyhydramnios, Transient Antenatal Bartter's Syndrome, and MAGED2 Mutations.

MAGE-D2 is a dynamic protein whose shuttling properties could suggest a role in cell cycle regulation.
Title: Melanoma antigen-D2: A nucleolar protein undergoing delocalization during cell cycle and after cellular stress.

Increased expression of MAGE-D2 mRNA was associated with distant metastasis in Gastric Cancer.
Title: The Expression of Melanoma-Associated Antigen D2 Both in Surgically Resected and Serum Samples Serves as Clinically Relevant Biomarker of Gastric Cancer Progression.

these results identify the expression of MAGE-D2 suppresses TRAIL receptor 2 and protects againstas TRAIL-induced apoptosis
Title: The melanoma-associated antigen MAGE-D2 suppresses TRAIL receptor 2 and protects against TRAIL-induced apoptosis in human melanoma cells.

Observational study of gene-disease association. (HuGE Navigator)
Title: New genetic associations detected in a host response study to hepatitis B vaccine.

The following MAGED2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MAGED2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu02833	NM_014599.6 Latest version!	Homo sapiens MAGE family member D2 (MAGED2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu02833	NM_177433.3 Latest version!	Homo sapiens MAGE family member D2 (MAGED2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu02833	NM_201222.3 Latest version!	Homo sapiens MAGE family member D2 (MAGED2), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
View Old Accession Versions >>
OHu02833	NM_177433.2	Homo sapiens MAGE family member D2 (MAGED2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu02833	NM_014599.5	Homo sapiens MAGE family member D2 (MAGED2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu02833	NM_201222.2	Homo sapiens MAGE family member D2 (MAGED2), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MAGED2 ( NM_177433.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_803182.1 Homo sapiens MAGE family member D2 (MAGED2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu02833
Clone ID Related Accession (Same CDS sequence)	NM_177433.2 , NM_201222.3 , NM_014599.5 , NM_177433.3 , NM_014599.6 , NM_201222.2
Accession Version	NM_177433.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1821bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-27
Organism	Homo sapiens(human)
Product	melanoma-associated antigen D2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB467873.1, BC000304.2 and BX647995.1. This sequence is a reference standard in the RefSeqGene project. On Aug 20, 2013 this sequence version replaced NM_177433.1. Transcript Variant: This variant (2) represents the longest transcript. Variants 1, 2, and 3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC091503.1, SRR1803616.232825.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCTGACA CAAGCGAGAG TGGTGCAGGT CTAACTCGCT TCCAGGCTGA AGCTTCAGAA 
AAGGACAGTA GCTCGATGAT GCAGACTCTG TTGACAGTGA CCCAGAATGT GGAGGTCCCA 
GAGACACCGA AGGCCTCAAA GGCACTGGAG GTCTCAGAGG ATGTGAAGGT CTCAAAAGCC 
TCTGGGGTCT CAAAGGCCAC AGAGGTCTCA AAGACCCCAG AGGCTCGGGA GGCACCTGCC 
ACCCAGGCCT CATCTACTAC TCAGCTGACT GATACCCAGG TTCTGGCAGC TGAAAACAAG 
AGTCTAGCAG CTGACACCAA GAAACAGAAT GCTGACCCGC AGGCTGTGAC AATGCCTGCC 
ACTGAGACCA AAAAGGTCAG CCATGTGGCT GATACAAAGG TCAATACAAA GGCTCAGGAG 
ACTGAGGCTG CACCCTCTCA GGCCCCAGCA GATGAACCTG AGCCTGAGAG TGCAGCTGCC 
CAGTCTCAGG AGAATCAGGA TACTCGGCCC AAGGTCAAAG CCAAGAAAGC CCGAAAGGTG 
AAGCATCTGG ATGGGGAAGA GGATGGCAGC AGTGATCAGA GTCAGGCTTC TGGAACCACA 
GGTGGCCGAA GGGTCTCAAA GGCCCTAATG GCCTCAATGG CCCGCAGGGC TTCAAGGGGT 
CCCATAGCCT TTTGGGCCCG CAGGGCATCA AGGACTCGGT TGGCTGCTTG GGCCCGGAGA 
GCCTTGCTCT CCCTGAGATC ACCTAAAGCC CGTAGGGGCA AGGCTCGCCG TAGAGCTGCC 
AAGCTCCAGT CATCCCAAGA GCCTGAAGCA CCACCACCTC GGGATGTGGC CCTTTTGCAA 
GGGAGGGCAA ATGATTTGGT GAAGTACCTT TTGGCTAAAG ACCAGACGAA GATTCCCATC 
AAGCGCTCGG ACATGCTGAA GGACATCATC AAAGAATACA CTGATGTGTA CCCCGAAATC 
ATTGAACGAG CAGGCTATTC CTTGGAGAAG GTATTTGGGA TTCAATTGAA GGAAATTGAT 
AAGAATGACC ACTTGTACAT TCTTCTCAGC ACCTTAGAGC CCACTGATGC AGGCATACTG 
GGAACGACTA AGGACTCACC CAAGCTGGGT CTGCTCATGG TGCTTCTTAG CATCATCTTC 
ATGAATGGAA ATCGGTCCAG TGAGGCTGTC ATCTGGGAGG TGCTGCGCAA GTTGGGGCTG 
CGCCCTGGGA TACATCATTC ACTCTTTGGG GACGTGAAGA AGCTCATCAC TGATGAGTTT 
GTGAAGCAGA AGTACCTGGA CTATGCCAGA GTCCCCAATA GCAATCCCCC TGAATATGAG 
TTCTTCTGGG GCCTGCGCTC TTACTATGAG ACCAGCAAGA TGAAAGTCCT CAAGTTTGCC 
TGCAAGGTAC AAAAGAAGGA TCCCAAGGAA TGGGCAGCTC AGTACCGAGA GGCGATGGAA 
GCGGATTTGA AGGCTGCAGC TGAGGCTGCA GCTGAAGCCA AGGCTAGGGC CGAGATTAGA 
GCTCGAATGG GCATTGGGCT CGGCTCGGAG AATGCTGCCG GGCCCTGCAA CTGGGACGAA 
GCTGATATCG GACCCTGGGC CAAAGCCCGG ATCCAGGCGG GAGCAGAAGC TAAAGCCAAA 
GCCCAAGAGA GTGGCAGTGC CAGCACTGGT GCCAGTACCA GTACCAATAA CAGTGCCAGT 
GCCAGTGCCA GCACCAGTGG TGGCTTCAGT GCTGGTGCCA GCCTGACCGC CACTCTCACA 
TTTGGGCTCT TCGCTGGCCT TGGTGGAGCT GGTGCCAGCA CCAGTGGCAG CTCTGGTGCC 
TGTGGTTTCT CCTACAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_803182.1
CDS	234..2054
Translation	MSDTSESGAGLTRFQAEASEKDSSSMMQTLLTVTQNVEVPETPKASKALEVSEDVKVSKASGVSKATEVSKTPEAREAPATQASSTTQLTDTQVLAAENKSLAADTKKQNADPQAVTMPATETKKVSHVADTKVNTKAQETEAAPSQAPADEPEPESAAAQSQENQDTRPKVKAKKARKVKHLDGEEDGSSDQSQASGTTGGRRVSKALMASMARRASRGPIAFWARRASRTRLAAWARRALLSLRSPKARRGKARRRAAKLQSSQEPEAPPPRDVALLQGRANDLVKYLLAKDQTKIPIKRSDMLKDIIKEYTDVYPEIIERAGYSLEKVFGIQLKEIDKNDHLYILLSTLEPTDAGILGTTKDSPKLGLLMVLLSIIFMNGNRSSEAVIWEVLRKLGLRPGIHHSLFGDVKKLITDEFVKQKYLDYARVPNSNPPEYEFFWGLRSYYETSKMKVLKFACKVQKKDPKEWAAQYREAMEADLKAAAEAAAEAKARAEIRARMGIGLGSENAAGPCNWDEADIGPWAKARIQAGAEAKAKAQESGSASTGASTSTNNSASASASTSGGFSAGASLTATLTFGLFAGLGGAGASTSGSSGACGFSYK

Target ORF information:

RefSeq Version	NM_177433.2
Organism	Homo sapiens(human)
Definition	Homo sapiens MAGE family member D2 (MAGED2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_177433.2

ORF Insert Sequence:

ATGTCTGACA CAAGCGAGAG TGGTGCAGGT CTAACTCGCT TCCAGGCTGA AGCTTCAGAA 
AAGGACAGTA GCTCGATGAT GCAGACTCTG TTGACAGTGA CCCAGAATGT GGAGGTCCCA 
GAGACACCGA AGGCCTCAAA GGCACTGGAG GTCTCAGAGG ATGTGAAGGT CTCAAAAGCC 
TCTGGGGTCT CAAAGGCCAC AGAGGTCTCA AAGACCCCAG AGGCTCGGGA GGCACCTGCC 
ACCCAGGCCT CATCTACTAC TCAGCTGACT GATACCCAGG TTCTGGCAGC TGAAAACAAG 
AGTCTAGCAG CTGACACCAA GAAACAGAAT GCTGACCCGC AGGCTGTGAC AATGCCTGCC 
ACTGAGACCA AAAAGGTCAG CCATGTGGCT GATACAAAGG TCAATACAAA GGCTCAGGAG 
ACTGAGGCTG CACCCTCTCA GGCCCCAGCA GATGAACCTG AGCCTGAGAG TGCAGCTGCC 
CAGTCTCAGG AGAATCAGGA TACTCGGCCC AAGGTCAAAG CCAAGAAAGC CCGAAAGGTG 
AAGCATCTGG ATGGGGAAGA GGATGGCAGC AGTGATCAGA GTCAGGCTTC TGGAACCACA 
GGTGGCCGAA GGGTCTCAAA GGCCCTAATG GCCTCAATGG CCCGCAGGGC TTCAAGGGGT 
CCCATAGCCT TTTGGGCCCG CAGGGCATCA AGGACTCGGT TGGCTGCTTG GGCCCGGAGA 
GCCTTGCTCT CCCTGAGATC ACCTAAAGCC CGTAGGGGCA AGGCTCGCCG TAGAGCTGCC 
AAGCTCCAGT CATCCCAAGA GCCTGAAGCA CCACCACCTC GGGATGTGGC CCTTTTGCAA 
GGGAGGGCAA ATGATTTGGT GAAGTACCTT TTGGCTAAAG ACCAGACGAA GATTCCCATC 
AAGCGCTCGG ACATGCTGAA GGACATCATC AAAGAATACA CTGATGTGTA CCCCGAAATC 
ATTGAACGAG CAGGCTATTC CTTGGAGAAG GTATTTGGGA TTCAATTGAA GGAAATTGAT 
AAGAATGACC ACTTGTACAT TCTTCTCAGC ACCTTAGAGC CCACTGATGC AGGCATACTG 
GGAACGACTA AGGACTCACC CAAGCTGGGT CTGCTCATGG TGCTTCTTAG CATCATCTTC 
ATGAATGGAA ATCGGTCCAG TGAGGCTGTC ATCTGGGAGG TGCTGCGCAA GTTGGGGCTG 
CGCCCTGGGA TACATCATTC ACTCTTTGGG GACGTGAAGA AGCTCATCAC TGATGAGTTT 
GTGAAGCAGA AGTACCTGGA CTATGCCAGA GTCCCCAATA GCAATCCCCC TGAATATGAG 
TTCTTCTGGG GCCTGCGCTC TTACTATGAG ACCAGCAAGA TGAAAGTCCT CAAGTTTGCC 
TGCAAGGTAC AAAAGAAGGA TCCCAAGGAA TGGGCAGCTC AGTACCGAGA GGCGATGGAA 
GCGGATTTGA AGGCTGCAGC TGAGGCTGCA GCTGAAGCCA AGGCTAGGGC CGAGATTAGA 
GCTCGAATGG GCATTGGGCT CGGCTCGGAG AATGCTGCCG GGCCCTGCAA CTGGGACGAA 
GCTGATATCG GACCCTGGGC CAAAGCCCGG ATCCAGGCGG GAGCAGAAGC TAAAGCCAAA 
GCCCAAGAGA GTGGCAGTGC CAGCACTGGT GCCAGTACCA GTACCAATAA CAGTGCCAGT 
GCCAGTGCCA GCACCAGTGG TGGCTTCAGT GCTGGTGCCA GCCTGACCGC CACTCTCACA 
TTTGGGCTCT TCGCTGGCCT TGGTGGAGCT GGTGCCAGCA CCAGTGGCAG CTCTGGTGCC 
TGTGGTTTCT CCTACAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAGED2 ( NM_201222.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_957516.1 Homo sapiens MAGE family member D2 (MAGED2), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu02833
Clone ID Related Accession (Same CDS sequence)	NM_177433.2 , NM_201222.3 , NM_014599.5 , NM_177433.3 , NM_014599.6 , NM_201222.2
Accession Version	NM_201222.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1821bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-27
Organism	Homo sapiens(human)
Product	melanoma-associated antigen D2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BM043994.1, BC000304.2 and BX647995.1. On Jun 1, 2019 this sequence version replaced NM_201222.2. Transcript Variant: This variant (3) differs in the 5' UTR, compared to variant 2. Variants 1, 2, and 3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR7346977.903912.1, BX647995.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCTGACA CAAGCGAGAG TGGTGCAGGT CTAACTCGCT TCCAGGCTGA AGCTTCAGAA 
AAGGACAGTA GCTCGATGAT GCAGACTCTG TTGACAGTGA CCCAGAATGT GGAGGTCCCA 
GAGACACCGA AGGCCTCAAA GGCACTGGAG GTCTCAGAGG ATGTGAAGGT CTCAAAAGCC 
TCTGGGGTCT CAAAGGCCAC AGAGGTCTCA AAGACCCCAG AGGCTCGGGA GGCACCTGCC 
ACCCAGGCCT CATCTACTAC TCAGCTGACT GATACCCAGG TTCTGGCAGC TGAAAACAAG 
AGTCTAGCAG CTGACACCAA GAAACAGAAT GCTGACCCGC AGGCTGTGAC AATGCCTGCC 
ACTGAGACCA AAAAGGTCAG CCATGTGGCT GATACAAAGG TCAATACAAA GGCTCAGGAG 
ACTGAGGCTG CACCCTCTCA GGCCCCAGCA GATGAACCTG AGCCTGAGAG TGCAGCTGCC 
CAGTCTCAGG AGAATCAGGA TACTCGGCCC AAGGTCAAAG CCAAGAAAGC CCGAAAGGTG 
AAGCATCTGG ATGGGGAAGA GGATGGCAGC AGTGATCAGA GTCAGGCTTC TGGAACCACA 
GGTGGCCGAA GGGTCTCAAA GGCCCTAATG GCCTCAATGG CCCGCAGGGC TTCAAGGGGT 
CCCATAGCCT TTTGGGCCCG CAGGGCATCA AGGACTCGGT TGGCTGCTTG GGCCCGGAGA 
GCCTTGCTCT CCCTGAGATC ACCTAAAGCC CGTAGGGGCA AGGCTCGCCG TAGAGCTGCC 
AAGCTCCAGT CATCCCAAGA GCCTGAAGCA CCACCACCTC GGGATGTGGC CCTTTTGCAA 
GGGAGGGCAA ATGATTTGGT GAAGTACCTT TTGGCTAAAG ACCAGACGAA GATTCCCATC 
AAGCGCTCGG ACATGCTGAA GGACATCATC AAAGAATACA CTGATGTGTA CCCCGAAATC 
ATTGAACGAG CAGGCTATTC CTTGGAGAAG GTATTTGGGA TTCAATTGAA GGAAATTGAT 
AAGAATGACC ACTTGTACAT TCTTCTCAGC ACCTTAGAGC CCACTGATGC AGGCATACTG 
GGAACGACTA AGGACTCACC CAAGCTGGGT CTGCTCATGG TGCTTCTTAG CATCATCTTC 
ATGAATGGAA ATCGGTCCAG TGAGGCTGTC ATCTGGGAGG TGCTGCGCAA GTTGGGGCTG 
CGCCCTGGGA TACATCATTC ACTCTTTGGG GACGTGAAGA AGCTCATCAC TGATGAGTTT 
GTGAAGCAGA AGTACCTGGA CTATGCCAGA GTCCCCAATA GCAATCCCCC TGAATATGAG 
TTCTTCTGGG GCCTGCGCTC TTACTATGAG ACCAGCAAGA TGAAAGTCCT CAAGTTTGCC 
TGCAAGGTAC AAAAGAAGGA TCCCAAGGAA TGGGCAGCTC AGTACCGAGA GGCGATGGAA 
GCGGATTTGA AGGCTGCAGC TGAGGCTGCA GCTGAAGCCA AGGCTAGGGC CGAGATTAGA 
GCTCGAATGG GCATTGGGCT CGGCTCGGAG AATGCTGCCG GGCCCTGCAA CTGGGACGAA 
GCTGATATCG GACCCTGGGC CAAAGCCCGG ATCCAGGCGG GAGCAGAAGC TAAAGCCAAA 
GCCCAAGAGA GTGGCAGTGC CAGCACTGGT GCCAGTACCA GTACCAATAA CAGTGCCAGT 
GCCAGTGCCA GCACCAGTGG TGGCTTCAGT GCTGGTGCCA GCCTGACCGC CACTCTCACA 
TTTGGGCTCT TCGCTGGCCT TGGTGGAGCT GGTGCCAGCA CCAGTGGCAG CTCTGGTGCC 
TGTGGTTTCT CCTACAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_957516.1
CDS	173..1993
Translation	MSDTSESGAGLTRFQAEASEKDSSSMMQTLLTVTQNVEVPETPKASKALEVSEDVKVSKASGVSKATEVSKTPEAREAPATQASSTTQLTDTQVLAAENKSLAADTKKQNADPQAVTMPATETKKVSHVADTKVNTKAQETEAAPSQAPADEPEPESAAAQSQENQDTRPKVKAKKARKVKHLDGEEDGSSDQSQASGTTGGRRVSKALMASMARRASRGPIAFWARRASRTRLAAWARRALLSLRSPKARRGKARRRAAKLQSSQEPEAPPPRDVALLQGRANDLVKYLLAKDQTKIPIKRSDMLKDIIKEYTDVYPEIIERAGYSLEKVFGIQLKEIDKNDHLYILLSTLEPTDAGILGTTKDSPKLGLLMVLLSIIFMNGNRSSEAVIWEVLRKLGLRPGIHHSLFGDVKKLITDEFVKQKYLDYARVPNSNPPEYEFFWGLRSYYETSKMKVLKFACKVQKKDPKEWAAQYREAMEADLKAAAEAAAEAKARAEIRARMGIGLGSENAAGPCNWDEADIGPWAKARIQAGAEAKAKAQESGSASTGASTSTNNSASASASTSGGFSAGASLTATLTFGLFAGLGGAGASTSGSSGACGFSYK

Target ORF information:

RefSeq Version	NM_201222.3
Organism	Homo sapiens(human)
Definition	Homo sapiens MAGE family member D2 (MAGED2), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_201222.3

ORF Insert Sequence:

ATGTCTGACA CAAGCGAGAG TGGTGCAGGT CTAACTCGCT TCCAGGCTGA AGCTTCAGAA 
AAGGACAGTA GCTCGATGAT GCAGACTCTG TTGACAGTGA CCCAGAATGT GGAGGTCCCA 
GAGACACCGA AGGCCTCAAA GGCACTGGAG GTCTCAGAGG ATGTGAAGGT CTCAAAAGCC 
TCTGGGGTCT CAAAGGCCAC AGAGGTCTCA AAGACCCCAG AGGCTCGGGA GGCACCTGCC 
ACCCAGGCCT CATCTACTAC TCAGCTGACT GATACCCAGG TTCTGGCAGC TGAAAACAAG 
AGTCTAGCAG CTGACACCAA GAAACAGAAT GCTGACCCGC AGGCTGTGAC AATGCCTGCC 
ACTGAGACCA AAAAGGTCAG CCATGTGGCT GATACAAAGG TCAATACAAA GGCTCAGGAG 
ACTGAGGCTG CACCCTCTCA GGCCCCAGCA GATGAACCTG AGCCTGAGAG TGCAGCTGCC 
CAGTCTCAGG AGAATCAGGA TACTCGGCCC AAGGTCAAAG CCAAGAAAGC CCGAAAGGTG 
AAGCATCTGG ATGGGGAAGA GGATGGCAGC AGTGATCAGA GTCAGGCTTC TGGAACCACA 
GGTGGCCGAA GGGTCTCAAA GGCCCTAATG GCCTCAATGG CCCGCAGGGC TTCAAGGGGT 
CCCATAGCCT TTTGGGCCCG CAGGGCATCA AGGACTCGGT TGGCTGCTTG GGCCCGGAGA 
GCCTTGCTCT CCCTGAGATC ACCTAAAGCC CGTAGGGGCA AGGCTCGCCG TAGAGCTGCC 
AAGCTCCAGT CATCCCAAGA GCCTGAAGCA CCACCACCTC GGGATGTGGC CCTTTTGCAA 
GGGAGGGCAA ATGATTTGGT GAAGTACCTT TTGGCTAAAG ACCAGACGAA GATTCCCATC 
AAGCGCTCGG ACATGCTGAA GGACATCATC AAAGAATACA CTGATGTGTA CCCCGAAATC 
ATTGAACGAG CAGGCTATTC CTTGGAGAAG GTATTTGGGA TTCAATTGAA GGAAATTGAT 
AAGAATGACC ACTTGTACAT TCTTCTCAGC ACCTTAGAGC CCACTGATGC AGGCATACTG 
GGAACGACTA AGGACTCACC CAAGCTGGGT CTGCTCATGG TGCTTCTTAG CATCATCTTC 
ATGAATGGAA ATCGGTCCAG TGAGGCTGTC ATCTGGGAGG TGCTGCGCAA GTTGGGGCTG 
CGCCCTGGGA TACATCATTC ACTCTTTGGG GACGTGAAGA AGCTCATCAC TGATGAGTTT 
GTGAAGCAGA AGTACCTGGA CTATGCCAGA GTCCCCAATA GCAATCCCCC TGAATATGAG 
TTCTTCTGGG GCCTGCGCTC TTACTATGAG ACCAGCAAGA TGAAAGTCCT CAAGTTTGCC 
TGCAAGGTAC AAAAGAAGGA TCCCAAGGAA TGGGCAGCTC AGTACCGAGA GGCGATGGAA 
GCGGATTTGA AGGCTGCAGC TGAGGCTGCA GCTGAAGCCA AGGCTAGGGC CGAGATTAGA 
GCTCGAATGG GCATTGGGCT CGGCTCGGAG AATGCTGCCG GGCCCTGCAA CTGGGACGAA 
GCTGATATCG GACCCTGGGC CAAAGCCCGG ATCCAGGCGG GAGCAGAAGC TAAAGCCAAA 
GCCCAAGAGA GTGGCAGTGC CAGCACTGGT GCCAGTACCA GTACCAATAA CAGTGCCAGT 
GCCAGTGCCA GCACCAGTGG TGGCTTCAGT GCTGGTGCCA GCCTGACCGC CACTCTCACA 
TTTGGGCTCT TCGCTGGCCT TGGTGGAGCT GGTGCCAGCA CCAGTGGCAG CTCTGGTGCC 
TGTGGTTTCT CCTACAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAGED2 ( NM_014599.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_055414.2 Homo sapiens MAGE family member D2 (MAGED2), transcript variant 1, mRNA.

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CloneID	OHu02833
Clone ID Related Accession (Same CDS sequence)	NM_177433.2 , NM_201222.3 , NM_014599.5 , NM_177433.3 , NM_014599.6 , NM_201222.2
Accession Version	NM_014599.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1821bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Homo sapiens(human)
Product	melanoma-associated antigen D2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from Z98046.1, HY017171.1, BC000304.2 and BX647995.1. On Aug 20, 2013 this sequence version replaced NM_014599.4. Transcript Variant: This variant (1) differs in the 5' UTR, compared to variant 2. Variants 1, 2, and 3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163655.131704.1, BC000304.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCTGACA CAAGCGAGAG TGGTGCAGGT CTAACTCGCT TCCAGGCTGA AGCTTCAGAA 
AAGGACAGTA GCTCGATGAT GCAGACTCTG TTGACAGTGA CCCAGAATGT GGAGGTCCCA 
GAGACACCGA AGGCCTCAAA GGCACTGGAG GTCTCAGAGG ATGTGAAGGT CTCAAAAGCC 
TCTGGGGTCT CAAAGGCCAC AGAGGTCTCA AAGACCCCAG AGGCTCGGGA GGCACCTGCC 
ACCCAGGCCT CATCTACTAC TCAGCTGACT GATACCCAGG TTCTGGCAGC TGAAAACAAG 
AGTCTAGCAG CTGACACCAA GAAACAGAAT GCTGACCCGC AGGCTGTGAC AATGCCTGCC 
ACTGAGACCA AAAAGGTCAG CCATGTGGCT GATACAAAGG TCAATACAAA GGCTCAGGAG 
ACTGAGGCTG CACCCTCTCA GGCCCCAGCA GATGAACCTG AGCCTGAGAG TGCAGCTGCC 
CAGTCTCAGG AGAATCAGGA TACTCGGCCC AAGGTCAAAG CCAAGAAAGC CCGAAAGGTG 
AAGCATCTGG ATGGGGAAGA GGATGGCAGC AGTGATCAGA GTCAGGCTTC TGGAACCACA 
GGTGGCCGAA GGGTCTCAAA GGCCCTAATG GCCTCAATGG CCCGCAGGGC TTCAAGGGGT 
CCCATAGCCT TTTGGGCCCG CAGGGCATCA AGGACTCGGT TGGCTGCTTG GGCCCGGAGA 
GCCTTGCTCT CCCTGAGATC ACCTAAAGCC CGTAGGGGCA AGGCTCGCCG TAGAGCTGCC 
AAGCTCCAGT CATCCCAAGA GCCTGAAGCA CCACCACCTC GGGATGTGGC CCTTTTGCAA 
GGGAGGGCAA ATGATTTGGT GAAGTACCTT TTGGCTAAAG ACCAGACGAA GATTCCCATC 
AAGCGCTCGG ACATGCTGAA GGACATCATC AAAGAATACA CTGATGTGTA CCCCGAAATC 
ATTGAACGAG CAGGCTATTC CTTGGAGAAG GTATTTGGGA TTCAATTGAA GGAAATTGAT 
AAGAATGACC ACTTGTACAT TCTTCTCAGC ACCTTAGAGC CCACTGATGC AGGCATACTG 
GGAACGACTA AGGACTCACC CAAGCTGGGT CTGCTCATGG TGCTTCTTAG CATCATCTTC 
ATGAATGGAA ATCGGTCCAG TGAGGCTGTC ATCTGGGAGG TGCTGCGCAA GTTGGGGCTG 
CGCCCTGGGA TACATCATTC ACTCTTTGGG GACGTGAAGA AGCTCATCAC TGATGAGTTT 
GTGAAGCAGA AGTACCTGGA CTATGCCAGA GTCCCCAATA GCAATCCCCC TGAATATGAG 
TTCTTCTGGG GCCTGCGCTC TTACTATGAG ACCAGCAAGA TGAAAGTCCT CAAGTTTGCC 
TGCAAGGTAC AAAAGAAGGA TCCCAAGGAA TGGGCAGCTC AGTACCGAGA GGCGATGGAA 
GCGGATTTGA AGGCTGCAGC TGAGGCTGCA GCTGAAGCCA AGGCTAGGGC CGAGATTAGA 
GCTCGAATGG GCATTGGGCT CGGCTCGGAG AATGCTGCCG GGCCCTGCAA CTGGGACGAA 
GCTGATATCG GACCCTGGGC CAAAGCCCGG ATCCAGGCGG GAGCAGAAGC TAAAGCCAAA 
GCCCAAGAGA GTGGCAGTGC CAGCACTGGT GCCAGTACCA GTACCAATAA CAGTGCCAGT 
GCCAGTGCCA GCACCAGTGG TGGCTTCAGT GCTGGTGCCA GCCTGACCGC CACTCTCACA 
TTTGGGCTCT TCGCTGGCCT TGGTGGAGCT GGTGCCAGCA CCAGTGGCAG CTCTGGTGCC 
TGTGGTTTCT CCTACAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055414.2
CDS	126..1946
Translation	MSDTSESGAGLTRFQAEASEKDSSSMMQTLLTVTQNVEVPETPKASKALEVSEDVKVSKASGVSKATEVSKTPEAREAPATQASSTTQLTDTQVLAAENKSLAADTKKQNADPQAVTMPATETKKVSHVADTKVNTKAQETEAAPSQAPADEPEPESAAAQSQENQDTRPKVKAKKARKVKHLDGEEDGSSDQSQASGTTGGRRVSKALMASMARRASRGPIAFWARRASRTRLAAWARRALLSLRSPKARRGKARRRAAKLQSSQEPEAPPPRDVALLQGRANDLVKYLLAKDQTKIPIKRSDMLKDIIKEYTDVYPEIIERAGYSLEKVFGIQLKEIDKNDHLYILLSTLEPTDAGILGTTKDSPKLGLLMVLLSIIFMNGNRSSEAVIWEVLRKLGLRPGIHHSLFGDVKKLITDEFVKQKYLDYARVPNSNPPEYEFFWGLRSYYETSKMKVLKFACKVQKKDPKEWAAQYREAMEADLKAAAEAAAEAKARAEIRARMGIGLGSENAAGPCNWDEADIGPWAKARIQAGAEAKAKAQESGSASTGASTSTNNSASASASTSGGFSAGASLTATLTFGLFAGLGGAGASTSGSSGACGFSYK

Target ORF information:

RefSeq Version	NM_014599.5
Organism	Homo sapiens(human)
Definition	Homo sapiens MAGE family member D2 (MAGED2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014599.5

ORF Insert Sequence:

ATGTCTGACA CAAGCGAGAG TGGTGCAGGT CTAACTCGCT TCCAGGCTGA AGCTTCAGAA 
AAGGACAGTA GCTCGATGAT GCAGACTCTG TTGACAGTGA CCCAGAATGT GGAGGTCCCA 
GAGACACCGA AGGCCTCAAA GGCACTGGAG GTCTCAGAGG ATGTGAAGGT CTCAAAAGCC 
TCTGGGGTCT CAAAGGCCAC AGAGGTCTCA AAGACCCCAG AGGCTCGGGA GGCACCTGCC 
ACCCAGGCCT CATCTACTAC TCAGCTGACT GATACCCAGG TTCTGGCAGC TGAAAACAAG 
AGTCTAGCAG CTGACACCAA GAAACAGAAT GCTGACCCGC AGGCTGTGAC AATGCCTGCC 
ACTGAGACCA AAAAGGTCAG CCATGTGGCT GATACAAAGG TCAATACAAA GGCTCAGGAG 
ACTGAGGCTG CACCCTCTCA GGCCCCAGCA GATGAACCTG AGCCTGAGAG TGCAGCTGCC 
CAGTCTCAGG AGAATCAGGA TACTCGGCCC AAGGTCAAAG CCAAGAAAGC CCGAAAGGTG 
AAGCATCTGG ATGGGGAAGA GGATGGCAGC AGTGATCAGA GTCAGGCTTC TGGAACCACA 
GGTGGCCGAA GGGTCTCAAA GGCCCTAATG GCCTCAATGG CCCGCAGGGC TTCAAGGGGT 
CCCATAGCCT TTTGGGCCCG CAGGGCATCA AGGACTCGGT TGGCTGCTTG GGCCCGGAGA 
GCCTTGCTCT CCCTGAGATC ACCTAAAGCC CGTAGGGGCA AGGCTCGCCG TAGAGCTGCC 
AAGCTCCAGT CATCCCAAGA GCCTGAAGCA CCACCACCTC GGGATGTGGC CCTTTTGCAA 
GGGAGGGCAA ATGATTTGGT GAAGTACCTT TTGGCTAAAG ACCAGACGAA GATTCCCATC 
AAGCGCTCGG ACATGCTGAA GGACATCATC AAAGAATACA CTGATGTGTA CCCCGAAATC 
ATTGAACGAG CAGGCTATTC CTTGGAGAAG GTATTTGGGA TTCAATTGAA GGAAATTGAT 
AAGAATGACC ACTTGTACAT TCTTCTCAGC ACCTTAGAGC CCACTGATGC AGGCATACTG 
GGAACGACTA AGGACTCACC CAAGCTGGGT CTGCTCATGG TGCTTCTTAG CATCATCTTC 
ATGAATGGAA ATCGGTCCAG TGAGGCTGTC ATCTGGGAGG TGCTGCGCAA GTTGGGGCTG 
CGCCCTGGGA TACATCATTC ACTCTTTGGG GACGTGAAGA AGCTCATCAC TGATGAGTTT 
GTGAAGCAGA AGTACCTGGA CTATGCCAGA GTCCCCAATA GCAATCCCCC TGAATATGAG 
TTCTTCTGGG GCCTGCGCTC TTACTATGAG ACCAGCAAGA TGAAAGTCCT CAAGTTTGCC 
TGCAAGGTAC AAAAGAAGGA TCCCAAGGAA TGGGCAGCTC AGTACCGAGA GGCGATGGAA 
GCGGATTTGA AGGCTGCAGC TGAGGCTGCA GCTGAAGCCA AGGCTAGGGC CGAGATTAGA 
GCTCGAATGG GCATTGGGCT CGGCTCGGAG AATGCTGCCG GGCCCTGCAA CTGGGACGAA 
GCTGATATCG GACCCTGGGC CAAAGCCCGG ATCCAGGCGG GAGCAGAAGC TAAAGCCAAA 
GCCCAAGAGA GTGGCAGTGC CAGCACTGGT GCCAGTACCA GTACCAATAA CAGTGCCAGT 
GCCAGTGCCA GCACCAGTGG TGGCTTCAGT GCTGGTGCCA GCCTGACCGC CACTCTCACA 
TTTGGGCTCT TCGCTGGCCT TGGTGGAGCT GGTGCCAGCA CCAGTGGCAG CTCTGGTGCC 
TGTGGTTTCT CCTACAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAGED2 ( NM_177433.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_803182.1 Homo sapiens MAGE family member D2 (MAGED2), transcript variant 2, mRNA.

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CloneID	OHu02833
Clone ID Related Accession (Same CDS sequence)	NM_177433.2 , NM_201222.3 , NM_014599.5 , NM_177433.3 , NM_014599.6 , NM_201222.2
Accession Version	NM_177433.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1821bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-26
Organism	Homo sapiens(human)
Product	melanoma-associated antigen D2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB467873.1, BC000304.2 and BX647995.1. This sequence is a reference standard in the RefSeqGene project. On Nov 24, 2018 this sequence version replaced NM_177433.2. Transcript Variant: This variant (2) represents the longest transcript. Variants 1, 2, and 3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.817157.1, SRR3476690.943828.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000375068.6/ ENSP00000364209.1 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTCTGACA CAAGCGAGAG TGGTGCAGGT CTAACTCGCT TCCAGGCTGA AGCTTCAGAA 
AAGGACAGTA GCTCGATGAT GCAGACTCTG TTGACAGTGA CCCAGAATGT GGAGGTCCCA 
GAGACACCGA AGGCCTCAAA GGCACTGGAG GTCTCAGAGG ATGTGAAGGT CTCAAAAGCC 
TCTGGGGTCT CAAAGGCCAC AGAGGTCTCA AAGACCCCAG AGGCTCGGGA GGCACCTGCC 
ACCCAGGCCT CATCTACTAC TCAGCTGACT GATACCCAGG TTCTGGCAGC TGAAAACAAG 
AGTCTAGCAG CTGACACCAA GAAACAGAAT GCTGACCCGC AGGCTGTGAC AATGCCTGCC 
ACTGAGACCA AAAAGGTCAG CCATGTGGCT GATACAAAGG TCAATACAAA GGCTCAGGAG 
ACTGAGGCTG CACCCTCTCA GGCCCCAGCA GATGAACCTG AGCCTGAGAG TGCAGCTGCC 
CAGTCTCAGG AGAATCAGGA TACTCGGCCC AAGGTCAAAG CCAAGAAAGC CCGAAAGGTG 
AAGCATCTGG ATGGGGAAGA GGATGGCAGC AGTGATCAGA GTCAGGCTTC TGGAACCACA 
GGTGGCCGAA GGGTCTCAAA GGCCCTAATG GCCTCAATGG CCCGCAGGGC TTCAAGGGGT 
CCCATAGCCT TTTGGGCCCG CAGGGCATCA AGGACTCGGT TGGCTGCTTG GGCCCGGAGA 
GCCTTGCTCT CCCTGAGATC ACCTAAAGCC CGTAGGGGCA AGGCTCGCCG TAGAGCTGCC 
AAGCTCCAGT CATCCCAAGA GCCTGAAGCA CCACCACCTC GGGATGTGGC CCTTTTGCAA 
GGGAGGGCAA ATGATTTGGT GAAGTACCTT TTGGCTAAAG ACCAGACGAA GATTCCCATC 
AAGCGCTCGG ACATGCTGAA GGACATCATC AAAGAATACA CTGATGTGTA CCCCGAAATC 
ATTGAACGAG CAGGCTATTC CTTGGAGAAG GTATTTGGGA TTCAATTGAA GGAAATTGAT 
AAGAATGACC ACTTGTACAT TCTTCTCAGC ACCTTAGAGC CCACTGATGC AGGCATACTG 
GGAACGACTA AGGACTCACC CAAGCTGGGT CTGCTCATGG TGCTTCTTAG CATCATCTTC 
ATGAATGGAA ATCGGTCCAG TGAGGCTGTC ATCTGGGAGG TGCTGCGCAA GTTGGGGCTG 
CGCCCTGGGA TACATCATTC ACTCTTTGGG GACGTGAAGA AGCTCATCAC TGATGAGTTT 
GTGAAGCAGA AGTACCTGGA CTATGCCAGA GTCCCCAATA GCAATCCCCC TGAATATGAG 
TTCTTCTGGG GCCTGCGCTC TTACTATGAG ACCAGCAAGA TGAAAGTCCT CAAGTTTGCC 
TGCAAGGTAC AAAAGAAGGA TCCCAAGGAA TGGGCAGCTC AGTACCGAGA GGCGATGGAA 
GCGGATTTGA AGGCTGCAGC TGAGGCTGCA GCTGAAGCCA AGGCTAGGGC CGAGATTAGA 
GCTCGAATGG GCATTGGGCT CGGCTCGGAG AATGCTGCCG GGCCCTGCAA CTGGGACGAA 
GCTGATATCG GACCCTGGGC CAAAGCCCGG ATCCAGGCGG GAGCAGAAGC TAAAGCCAAA 
GCCCAAGAGA GTGGCAGTGC CAGCACTGGT GCCAGTACCA GTACCAATAA CAGTGCCAGT 
GCCAGTGCCA GCACCAGTGG TGGCTTCAGT GCTGGTGCCA GCCTGACCGC CACTCTCACA 
TTTGGGCTCT TCGCTGGCCT TGGTGGAGCT GGTGCCAGCA CCAGTGGCAG CTCTGGTGCC 
TGTGGTTTCT CCTACAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_803182.1
CDS	88..1908
Translation	MSDTSESGAGLTRFQAEASEKDSSSMMQTLLTVTQNVEVPETPKASKALEVSEDVKVSKASGVSKATEVSKTPEAREAPATQASSTTQLTDTQVLAAENKSLAADTKKQNADPQAVTMPATETKKVSHVADTKVNTKAQETEAAPSQAPADEPEPESAAAQSQENQDTRPKVKAKKARKVKHLDGEEDGSSDQSQASGTTGGRRVSKALMASMARRASRGPIAFWARRASRTRLAAWARRALLSLRSPKARRGKARRRAAKLQSSQEPEAPPPRDVALLQGRANDLVKYLLAKDQTKIPIKRSDMLKDIIKEYTDVYPEIIERAGYSLEKVFGIQLKEIDKNDHLYILLSTLEPTDAGILGTTKDSPKLGLLMVLLSIIFMNGNRSSEAVIWEVLRKLGLRPGIHHSLFGDVKKLITDEFVKQKYLDYARVPNSNPPEYEFFWGLRSYYETSKMKVLKFACKVQKKDPKEWAAQYREAMEADLKAAAEAAAEAKARAEIRARMGIGLGSENAAGPCNWDEADIGPWAKARIQAGAEAKAKAQESGSASTGASTSTNNSASASASTSGGFSAGASLTATLTFGLFAGLGGAGASTSGSSGACGFSYK

Target ORF information:

RefSeq Version	NM_177433.3
Organism	Homo sapiens(human)
Definition	Homo sapiens MAGE family member D2 (MAGED2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_177433.3

ORF Insert Sequence:

ATGTCTGACA CAAGCGAGAG TGGTGCAGGT CTAACTCGCT TCCAGGCTGA AGCTTCAGAA 
AAGGACAGTA GCTCGATGAT GCAGACTCTG TTGACAGTGA CCCAGAATGT GGAGGTCCCA 
GAGACACCGA AGGCCTCAAA GGCACTGGAG GTCTCAGAGG ATGTGAAGGT CTCAAAAGCC 
TCTGGGGTCT CAAAGGCCAC AGAGGTCTCA AAGACCCCAG AGGCTCGGGA GGCACCTGCC 
ACCCAGGCCT CATCTACTAC TCAGCTGACT GATACCCAGG TTCTGGCAGC TGAAAACAAG 
AGTCTAGCAG CTGACACCAA GAAACAGAAT GCTGACCCGC AGGCTGTGAC AATGCCTGCC 
ACTGAGACCA AAAAGGTCAG CCATGTGGCT GATACAAAGG TCAATACAAA GGCTCAGGAG 
ACTGAGGCTG CACCCTCTCA GGCCCCAGCA GATGAACCTG AGCCTGAGAG TGCAGCTGCC 
CAGTCTCAGG AGAATCAGGA TACTCGGCCC AAGGTCAAAG CCAAGAAAGC CCGAAAGGTG 
AAGCATCTGG ATGGGGAAGA GGATGGCAGC AGTGATCAGA GTCAGGCTTC TGGAACCACA 
GGTGGCCGAA GGGTCTCAAA GGCCCTAATG GCCTCAATGG CCCGCAGGGC TTCAAGGGGT 
CCCATAGCCT TTTGGGCCCG CAGGGCATCA AGGACTCGGT TGGCTGCTTG GGCCCGGAGA 
GCCTTGCTCT CCCTGAGATC ACCTAAAGCC CGTAGGGGCA AGGCTCGCCG TAGAGCTGCC 
AAGCTCCAGT CATCCCAAGA GCCTGAAGCA CCACCACCTC GGGATGTGGC CCTTTTGCAA 
GGGAGGGCAA ATGATTTGGT GAAGTACCTT TTGGCTAAAG ACCAGACGAA GATTCCCATC 
AAGCGCTCGG ACATGCTGAA GGACATCATC AAAGAATACA CTGATGTGTA CCCCGAAATC 
ATTGAACGAG CAGGCTATTC CTTGGAGAAG GTATTTGGGA TTCAATTGAA GGAAATTGAT 
AAGAATGACC ACTTGTACAT TCTTCTCAGC ACCTTAGAGC CCACTGATGC AGGCATACTG 
GGAACGACTA AGGACTCACC CAAGCTGGGT CTGCTCATGG TGCTTCTTAG CATCATCTTC 
ATGAATGGAA ATCGGTCCAG TGAGGCTGTC ATCTGGGAGG TGCTGCGCAA GTTGGGGCTG 
CGCCCTGGGA TACATCATTC ACTCTTTGGG GACGTGAAGA AGCTCATCAC TGATGAGTTT 
GTGAAGCAGA AGTACCTGGA CTATGCCAGA GTCCCCAATA GCAATCCCCC TGAATATGAG 
TTCTTCTGGG GCCTGCGCTC TTACTATGAG ACCAGCAAGA TGAAAGTCCT CAAGTTTGCC 
TGCAAGGTAC AAAAGAAGGA TCCCAAGGAA TGGGCAGCTC AGTACCGAGA GGCGATGGAA 
GCGGATTTGA AGGCTGCAGC TGAGGCTGCA GCTGAAGCCA AGGCTAGGGC CGAGATTAGA 
GCTCGAATGG GCATTGGGCT CGGCTCGGAG AATGCTGCCG GGCCCTGCAA CTGGGACGAA 
GCTGATATCG GACCCTGGGC CAAAGCCCGG ATCCAGGCGG GAGCAGAAGC TAAAGCCAAA 
GCCCAAGAGA GTGGCAGTGC CAGCACTGGT GCCAGTACCA GTACCAATAA CAGTGCCAGT 
GCCAGTGCCA GCACCAGTGG TGGCTTCAGT GCTGGTGCCA GCCTGACCGC CACTCTCACA 
TTTGGGCTCT TCGCTGGCCT TGGTGGAGCT GGTGCCAGCA CCAGTGGCAG CTCTGGTGCC 
TGTGGTTTCT CCTACAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAGED2 ( NM_014599.6 ) cDNA ORF clone, Homo sapiens(human) -> NP_055414.2 Homo sapiens MAGE family member D2 (MAGED2), transcript variant 1, mRNA.

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CloneID	OHu02833
Clone ID Related Accession (Same CDS sequence)	NM_177433.2 , NM_201222.3 , NM_014599.5 , NM_177433.3 , NM_014599.6 , NM_201222.2
Accession Version	NM_014599.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1821bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-27
Organism	Homo sapiens(human)
Product	melanoma-associated antigen D2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY017171.1, BC000304.2 and BX647995.1. On May 31, 2019 this sequence version replaced NM_014599.5. Transcript Variant: This variant (1) differs in the 5' UTR, compared to variant 2. Variants 1, 2, and 3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.1105167.1, SRR1803614.29975.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCTGACA CAAGCGAGAG TGGTGCAGGT CTAACTCGCT TCCAGGCTGA AGCTTCAGAA 
AAGGACAGTA GCTCGATGAT GCAGACTCTG TTGACAGTGA CCCAGAATGT GGAGGTCCCA 
GAGACACCGA AGGCCTCAAA GGCACTGGAG GTCTCAGAGG ATGTGAAGGT CTCAAAAGCC 
TCTGGGGTCT CAAAGGCCAC AGAGGTCTCA AAGACCCCAG AGGCTCGGGA GGCACCTGCC 
ACCCAGGCCT CATCTACTAC TCAGCTGACT GATACCCAGG TTCTGGCAGC TGAAAACAAG 
AGTCTAGCAG CTGACACCAA GAAACAGAAT GCTGACCCGC AGGCTGTGAC AATGCCTGCC 
ACTGAGACCA AAAAGGTCAG CCATGTGGCT GATACAAAGG TCAATACAAA GGCTCAGGAG 
ACTGAGGCTG CACCCTCTCA GGCCCCAGCA GATGAACCTG AGCCTGAGAG TGCAGCTGCC 
CAGTCTCAGG AGAATCAGGA TACTCGGCCC AAGGTCAAAG CCAAGAAAGC CCGAAAGGTG 
AAGCATCTGG ATGGGGAAGA GGATGGCAGC AGTGATCAGA GTCAGGCTTC TGGAACCACA 
GGTGGCCGAA GGGTCTCAAA GGCCCTAATG GCCTCAATGG CCCGCAGGGC TTCAAGGGGT 
CCCATAGCCT TTTGGGCCCG CAGGGCATCA AGGACTCGGT TGGCTGCTTG GGCCCGGAGA 
GCCTTGCTCT CCCTGAGATC ACCTAAAGCC CGTAGGGGCA AGGCTCGCCG TAGAGCTGCC 
AAGCTCCAGT CATCCCAAGA GCCTGAAGCA CCACCACCTC GGGATGTGGC CCTTTTGCAA 
GGGAGGGCAA ATGATTTGGT GAAGTACCTT TTGGCTAAAG ACCAGACGAA GATTCCCATC 
AAGCGCTCGG ACATGCTGAA GGACATCATC AAAGAATACA CTGATGTGTA CCCCGAAATC 
ATTGAACGAG CAGGCTATTC CTTGGAGAAG GTATTTGGGA TTCAATTGAA GGAAATTGAT 
AAGAATGACC ACTTGTACAT TCTTCTCAGC ACCTTAGAGC CCACTGATGC AGGCATACTG 
GGAACGACTA AGGACTCACC CAAGCTGGGT CTGCTCATGG TGCTTCTTAG CATCATCTTC 
ATGAATGGAA ATCGGTCCAG TGAGGCTGTC ATCTGGGAGG TGCTGCGCAA GTTGGGGCTG 
CGCCCTGGGA TACATCATTC ACTCTTTGGG GACGTGAAGA AGCTCATCAC TGATGAGTTT 
GTGAAGCAGA AGTACCTGGA CTATGCCAGA GTCCCCAATA GCAATCCCCC TGAATATGAG 
TTCTTCTGGG GCCTGCGCTC TTACTATGAG ACCAGCAAGA TGAAAGTCCT CAAGTTTGCC 
TGCAAGGTAC AAAAGAAGGA TCCCAAGGAA TGGGCAGCTC AGTACCGAGA GGCGATGGAA 
GCGGATTTGA AGGCTGCAGC TGAGGCTGCA GCTGAAGCCA AGGCTAGGGC CGAGATTAGA 
GCTCGAATGG GCATTGGGCT CGGCTCGGAG AATGCTGCCG GGCCCTGCAA CTGGGACGAA 
GCTGATATCG GACCCTGGGC CAAAGCCCGG ATCCAGGCGG GAGCAGAAGC TAAAGCCAAA 
GCCCAAGAGA GTGGCAGTGC CAGCACTGGT GCCAGTACCA GTACCAATAA CAGTGCCAGT 
GCCAGTGCCA GCACCAGTGG TGGCTTCAGT GCTGGTGCCA GCCTGACCGC CACTCTCACA 
TTTGGGCTCT TCGCTGGCCT TGGTGGAGCT GGTGCCAGCA CCAGTGGCAG CTCTGGTGCC 
TGTGGTTTCT CCTACAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055414.2
CDS	118..1938
Translation	MSDTSESGAGLTRFQAEASEKDSSSMMQTLLTVTQNVEVPETPKASKALEVSEDVKVSKASGVSKATEVSKTPEAREAPATQASSTTQLTDTQVLAAENKSLAADTKKQNADPQAVTMPATETKKVSHVADTKVNTKAQETEAAPSQAPADEPEPESAAAQSQENQDTRPKVKAKKARKVKHLDGEEDGSSDQSQASGTTGGRRVSKALMASMARRASRGPIAFWARRASRTRLAAWARRALLSLRSPKARRGKARRRAAKLQSSQEPEAPPPRDVALLQGRANDLVKYLLAKDQTKIPIKRSDMLKDIIKEYTDVYPEIIERAGYSLEKVFGIQLKEIDKNDHLYILLSTLEPTDAGILGTTKDSPKLGLLMVLLSIIFMNGNRSSEAVIWEVLRKLGLRPGIHHSLFGDVKKLITDEFVKQKYLDYARVPNSNPPEYEFFWGLRSYYETSKMKVLKFACKVQKKDPKEWAAQYREAMEADLKAAAEAAAEAKARAEIRARMGIGLGSENAAGPCNWDEADIGPWAKARIQAGAEAKAKAQESGSASTGASTSTNNSASASASTSGGFSAGASLTATLTFGLFAGLGGAGASTSGSSGACGFSYK

Target ORF information:

RefSeq Version	NM_014599.6
Organism	Homo sapiens(human)
Definition	Homo sapiens MAGE family member D2 (MAGED2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014599.6

ORF Insert Sequence:

ATGTCTGACA CAAGCGAGAG TGGTGCAGGT CTAACTCGCT TCCAGGCTGA AGCTTCAGAA 
AAGGACAGTA GCTCGATGAT GCAGACTCTG TTGACAGTGA CCCAGAATGT GGAGGTCCCA 
GAGACACCGA AGGCCTCAAA GGCACTGGAG GTCTCAGAGG ATGTGAAGGT CTCAAAAGCC 
TCTGGGGTCT CAAAGGCCAC AGAGGTCTCA AAGACCCCAG AGGCTCGGGA GGCACCTGCC 
ACCCAGGCCT CATCTACTAC TCAGCTGACT GATACCCAGG TTCTGGCAGC TGAAAACAAG 
AGTCTAGCAG CTGACACCAA GAAACAGAAT GCTGACCCGC AGGCTGTGAC AATGCCTGCC 
ACTGAGACCA AAAAGGTCAG CCATGTGGCT GATACAAAGG TCAATACAAA GGCTCAGGAG 
ACTGAGGCTG CACCCTCTCA GGCCCCAGCA GATGAACCTG AGCCTGAGAG TGCAGCTGCC 
CAGTCTCAGG AGAATCAGGA TACTCGGCCC AAGGTCAAAG CCAAGAAAGC CCGAAAGGTG 
AAGCATCTGG ATGGGGAAGA GGATGGCAGC AGTGATCAGA GTCAGGCTTC TGGAACCACA 
GGTGGCCGAA GGGTCTCAAA GGCCCTAATG GCCTCAATGG CCCGCAGGGC TTCAAGGGGT 
CCCATAGCCT TTTGGGCCCG CAGGGCATCA AGGACTCGGT TGGCTGCTTG GGCCCGGAGA 
GCCTTGCTCT CCCTGAGATC ACCTAAAGCC CGTAGGGGCA AGGCTCGCCG TAGAGCTGCC 
AAGCTCCAGT CATCCCAAGA GCCTGAAGCA CCACCACCTC GGGATGTGGC CCTTTTGCAA 
GGGAGGGCAA ATGATTTGGT GAAGTACCTT TTGGCTAAAG ACCAGACGAA GATTCCCATC 
AAGCGCTCGG ACATGCTGAA GGACATCATC AAAGAATACA CTGATGTGTA CCCCGAAATC 
ATTGAACGAG CAGGCTATTC CTTGGAGAAG GTATTTGGGA TTCAATTGAA GGAAATTGAT 
AAGAATGACC ACTTGTACAT TCTTCTCAGC ACCTTAGAGC CCACTGATGC AGGCATACTG 
GGAACGACTA AGGACTCACC CAAGCTGGGT CTGCTCATGG TGCTTCTTAG CATCATCTTC 
ATGAATGGAA ATCGGTCCAG TGAGGCTGTC ATCTGGGAGG TGCTGCGCAA GTTGGGGCTG 
CGCCCTGGGA TACATCATTC ACTCTTTGGG GACGTGAAGA AGCTCATCAC TGATGAGTTT 
GTGAAGCAGA AGTACCTGGA CTATGCCAGA GTCCCCAATA GCAATCCCCC TGAATATGAG 
TTCTTCTGGG GCCTGCGCTC TTACTATGAG ACCAGCAAGA TGAAAGTCCT CAAGTTTGCC 
TGCAAGGTAC AAAAGAAGGA TCCCAAGGAA TGGGCAGCTC AGTACCGAGA GGCGATGGAA 
GCGGATTTGA AGGCTGCAGC TGAGGCTGCA GCTGAAGCCA AGGCTAGGGC CGAGATTAGA 
GCTCGAATGG GCATTGGGCT CGGCTCGGAG AATGCTGCCG GGCCCTGCAA CTGGGACGAA 
GCTGATATCG GACCCTGGGC CAAAGCCCGG ATCCAGGCGG GAGCAGAAGC TAAAGCCAAA 
GCCCAAGAGA GTGGCAGTGC CAGCACTGGT GCCAGTACCA GTACCAATAA CAGTGCCAGT 
GCCAGTGCCA GCACCAGTGG TGGCTTCAGT GCTGGTGCCA GCCTGACCGC CACTCTCACA 
TTTGGGCTCT TCGCTGGCCT TGGTGGAGCT GGTGCCAGCA CCAGTGGCAG CTCTGGTGCC 
TGTGGTTTCT CCTACAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAGED2 ( NM_201222.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_957516.1 Homo sapiens MAGE family member D2 (MAGED2), transcript variant 3, mRNA.

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CloneID	OHu02833
Clone ID Related Accession (Same CDS sequence)	NM_177433.2 , NM_201222.3 , NM_014599.5 , NM_177433.3 , NM_014599.6 , NM_201222.2
Accession Version	NM_201222.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1821bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Homo sapiens(human)
Product	melanoma-associated antigen D2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BM043994.1, BC000304.2 and BX647995.1. On Aug 20, 2013 this sequence version replaced NM_201222.1. Transcript Variant: This variant (3) differs in the 5' UTR, compared to variant 2. Variants 1, 2, and 3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BX647995.1, SRR1660803.23518.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCTGACA CAAGCGAGAG TGGTGCAGGT CTAACTCGCT TCCAGGCTGA AGCTTCAGAA 
AAGGACAGTA GCTCGATGAT GCAGACTCTG TTGACAGTGA CCCAGAATGT GGAGGTCCCA 
GAGACACCGA AGGCCTCAAA GGCACTGGAG GTCTCAGAGG ATGTGAAGGT CTCAAAAGCC 
TCTGGGGTCT CAAAGGCCAC AGAGGTCTCA AAGACCCCAG AGGCTCGGGA GGCACCTGCC 
ACCCAGGCCT CATCTACTAC TCAGCTGACT GATACCCAGG TTCTGGCAGC TGAAAACAAG 
AGTCTAGCAG CTGACACCAA GAAACAGAAT GCTGACCCGC AGGCTGTGAC AATGCCTGCC 
ACTGAGACCA AAAAGGTCAG CCATGTGGCT GATACAAAGG TCAATACAAA GGCTCAGGAG 
ACTGAGGCTG CACCCTCTCA GGCCCCAGCA GATGAACCTG AGCCTGAGAG TGCAGCTGCC 
CAGTCTCAGG AGAATCAGGA TACTCGGCCC AAGGTCAAAG CCAAGAAAGC CCGAAAGGTG 
AAGCATCTGG ATGGGGAAGA GGATGGCAGC AGTGATCAGA GTCAGGCTTC TGGAACCACA 
GGTGGCCGAA GGGTCTCAAA GGCCCTAATG GCCTCAATGG CCCGCAGGGC TTCAAGGGGT 
CCCATAGCCT TTTGGGCCCG CAGGGCATCA AGGACTCGGT TGGCTGCTTG GGCCCGGAGA 
GCCTTGCTCT CCCTGAGATC ACCTAAAGCC CGTAGGGGCA AGGCTCGCCG TAGAGCTGCC 
AAGCTCCAGT CATCCCAAGA GCCTGAAGCA CCACCACCTC GGGATGTGGC CCTTTTGCAA 
GGGAGGGCAA ATGATTTGGT GAAGTACCTT TTGGCTAAAG ACCAGACGAA GATTCCCATC 
AAGCGCTCGG ACATGCTGAA GGACATCATC AAAGAATACA CTGATGTGTA CCCCGAAATC 
ATTGAACGAG CAGGCTATTC CTTGGAGAAG GTATTTGGGA TTCAATTGAA GGAAATTGAT 
AAGAATGACC ACTTGTACAT TCTTCTCAGC ACCTTAGAGC CCACTGATGC AGGCATACTG 
GGAACGACTA AGGACTCACC CAAGCTGGGT CTGCTCATGG TGCTTCTTAG CATCATCTTC 
ATGAATGGAA ATCGGTCCAG TGAGGCTGTC ATCTGGGAGG TGCTGCGCAA GTTGGGGCTG 
CGCCCTGGGA TACATCATTC ACTCTTTGGG GACGTGAAGA AGCTCATCAC TGATGAGTTT 
GTGAAGCAGA AGTACCTGGA CTATGCCAGA GTCCCCAATA GCAATCCCCC TGAATATGAG 
TTCTTCTGGG GCCTGCGCTC TTACTATGAG ACCAGCAAGA TGAAAGTCCT CAAGTTTGCC 
TGCAAGGTAC AAAAGAAGGA TCCCAAGGAA TGGGCAGCTC AGTACCGAGA GGCGATGGAA 
GCGGATTTGA AGGCTGCAGC TGAGGCTGCA GCTGAAGCCA AGGCTAGGGC CGAGATTAGA 
GCTCGAATGG GCATTGGGCT CGGCTCGGAG AATGCTGCCG GGCCCTGCAA CTGGGACGAA 
GCTGATATCG GACCCTGGGC CAAAGCCCGG ATCCAGGCGG GAGCAGAAGC TAAAGCCAAA 
GCCCAAGAGA GTGGCAGTGC CAGCACTGGT GCCAGTACCA GTACCAATAA CAGTGCCAGT 
GCCAGTGCCA GCACCAGTGG TGGCTTCAGT GCTGGTGCCA GCCTGACCGC CACTCTCACA 
TTTGGGCTCT TCGCTGGCCT TGGTGGAGCT GGTGCCAGCA CCAGTGGCAG CTCTGGTGCC 
TGTGGTTTCT CCTACAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_957516.1
CDS	194..2014
Translation	MSDTSESGAGLTRFQAEASEKDSSSMMQTLLTVTQNVEVPETPKASKALEVSEDVKVSKASGVSKATEVSKTPEAREAPATQASSTTQLTDTQVLAAENKSLAADTKKQNADPQAVTMPATETKKVSHVADTKVNTKAQETEAAPSQAPADEPEPESAAAQSQENQDTRPKVKAKKARKVKHLDGEEDGSSDQSQASGTTGGRRVSKALMASMARRASRGPIAFWARRASRTRLAAWARRALLSLRSPKARRGKARRRAAKLQSSQEPEAPPPRDVALLQGRANDLVKYLLAKDQTKIPIKRSDMLKDIIKEYTDVYPEIIERAGYSLEKVFGIQLKEIDKNDHLYILLSTLEPTDAGILGTTKDSPKLGLLMVLLSIIFMNGNRSSEAVIWEVLRKLGLRPGIHHSLFGDVKKLITDEFVKQKYLDYARVPNSNPPEYEFFWGLRSYYETSKMKVLKFACKVQKKDPKEWAAQYREAMEADLKAAAEAAAEAKARAEIRARMGIGLGSENAAGPCNWDEADIGPWAKARIQAGAEAKAKAQESGSASTGASTSTNNSASASASTSGGFSAGASLTATLTFGLFAGLGGAGASTSGSSGACGFSYK

Target ORF information:

RefSeq Version	NM_201222.2
Organism	Homo sapiens(human)
Definition	Homo sapiens MAGE family member D2 (MAGED2), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_201222.2

ORF Insert Sequence:

ATGTCTGACA CAAGCGAGAG TGGTGCAGGT CTAACTCGCT TCCAGGCTGA AGCTTCAGAA 
AAGGACAGTA GCTCGATGAT GCAGACTCTG TTGACAGTGA CCCAGAATGT GGAGGTCCCA 
GAGACACCGA AGGCCTCAAA GGCACTGGAG GTCTCAGAGG ATGTGAAGGT CTCAAAAGCC 
TCTGGGGTCT CAAAGGCCAC AGAGGTCTCA AAGACCCCAG AGGCTCGGGA GGCACCTGCC 
ACCCAGGCCT CATCTACTAC TCAGCTGACT GATACCCAGG TTCTGGCAGC TGAAAACAAG 
AGTCTAGCAG CTGACACCAA GAAACAGAAT GCTGACCCGC AGGCTGTGAC AATGCCTGCC 
ACTGAGACCA AAAAGGTCAG CCATGTGGCT GATACAAAGG TCAATACAAA GGCTCAGGAG 
ACTGAGGCTG CACCCTCTCA GGCCCCAGCA GATGAACCTG AGCCTGAGAG TGCAGCTGCC 
CAGTCTCAGG AGAATCAGGA TACTCGGCCC AAGGTCAAAG CCAAGAAAGC CCGAAAGGTG 
AAGCATCTGG ATGGGGAAGA GGATGGCAGC AGTGATCAGA GTCAGGCTTC TGGAACCACA 
GGTGGCCGAA GGGTCTCAAA GGCCCTAATG GCCTCAATGG CCCGCAGGGC TTCAAGGGGT 
CCCATAGCCT TTTGGGCCCG CAGGGCATCA AGGACTCGGT TGGCTGCTTG GGCCCGGAGA 
GCCTTGCTCT CCCTGAGATC ACCTAAAGCC CGTAGGGGCA AGGCTCGCCG TAGAGCTGCC 
AAGCTCCAGT CATCCCAAGA GCCTGAAGCA CCACCACCTC GGGATGTGGC CCTTTTGCAA 
GGGAGGGCAA ATGATTTGGT GAAGTACCTT TTGGCTAAAG ACCAGACGAA GATTCCCATC 
AAGCGCTCGG ACATGCTGAA GGACATCATC AAAGAATACA CTGATGTGTA CCCCGAAATC 
ATTGAACGAG CAGGCTATTC CTTGGAGAAG GTATTTGGGA TTCAATTGAA GGAAATTGAT 
AAGAATGACC ACTTGTACAT TCTTCTCAGC ACCTTAGAGC CCACTGATGC AGGCATACTG 
GGAACGACTA AGGACTCACC CAAGCTGGGT CTGCTCATGG TGCTTCTTAG CATCATCTTC 
ATGAATGGAA ATCGGTCCAG TGAGGCTGTC ATCTGGGAGG TGCTGCGCAA GTTGGGGCTG 
CGCCCTGGGA TACATCATTC ACTCTTTGGG GACGTGAAGA AGCTCATCAC TGATGAGTTT 
GTGAAGCAGA AGTACCTGGA CTATGCCAGA GTCCCCAATA GCAATCCCCC TGAATATGAG 
TTCTTCTGGG GCCTGCGCTC TTACTATGAG ACCAGCAAGA TGAAAGTCCT CAAGTTTGCC 
TGCAAGGTAC AAAAGAAGGA TCCCAAGGAA TGGGCAGCTC AGTACCGAGA GGCGATGGAA 
GCGGATTTGA AGGCTGCAGC TGAGGCTGCA GCTGAAGCCA AGGCTAGGGC CGAGATTAGA 
GCTCGAATGG GCATTGGGCT CGGCTCGGAG AATGCTGCCG GGCCCTGCAA CTGGGACGAA 
GCTGATATCG GACCCTGGGC CAAAGCCCGG ATCCAGGCGG GAGCAGAAGC TAAAGCCAAA 
GCCCAAGAGA GTGGCAGTGC CAGCACTGGT GCCAGTACCA GTACCAATAA CAGTGCCAGT 
GCCAGTGCCA GCACCAGTGG TGGCTTCAGT GCTGGTGCCA GCCTGACCGC CACTCTCACA 
TTTGGGCTCT TCGCTGGCCT TGGTGGAGCT GGTGCCAGCA CCAGTGGCAG CTCTGGTGCC 
TGTGGTTTCT CCTACAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Prevalence of Novel
Clinical journal of the American Society of Nephrology : CJASN(2017 Nov)
Legrand A,Treard C,Roncelin I,Dreux S,Bertholet-Thomas A,Broux F,Bruno D,Decramer S,Deschenes G,Djeddi D,Guigonis V,Jay N,Khalifeh T,Llanas B,Morin D,Morin G,Nobili F,Pietrement C,Ryckewaert A,Salomon R,Vrillon I,Blanchard A,Vargas-Poussou R

Polyhydramnios, Transient Antenatal Bartter's Syndrome, and MAGED2 Mutations.
The New England journal of medicine374(19)1853-63(2016 May)
Laghmani K,Beck BB,Yang SS,Seaayfan E,Wenzel A,Reusch B,Vitzthum H,Priem D,Demaretz S,Bergmann K,Duin LK,G?bel H,Mache C,Thiele H,Bartram MP,Dombret C,Altm?ller J,N?rnberg P,Benzing T,Levtchenko E,Seyberth HW,Klaus G,Yigit G,Lin SH,Timmer A,de Koning TJ,Scherjon SA,Schlingmann KP,Bertrand MJ,Rinschen MM,de Backer O,Konrad M,K?mhoff M

Melanoma antigen-D2: A nucleolar protein undergoing delocalization during cell cycle and after cellular stress.
Biochimica et biophysica acta1863(4)581-95(2016 Apr)
Pirlot C,Thiry M,Trussart C,Di Valentin E,Piette J,Habraken Y

Integrative analysis of kinase networks in TRAIL-induced apoptosis provides a source of potential targets for combination therapy.
Science signaling8(371)rs3(2015 Apr)
So J,Pasculescu A,Dai AY,Williton K,James A,Nguyen V,Creixell P,Schoof EM,Sinclair J,Barrios-Rodiles M,Gu J,Krizus A,Williams R,Olhovsky M,Dennis JW,Wrana JL,Linding R,Jorgensen C,Pawson T,Colwill K

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MAGED2 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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