PAPOLA gene cDNA ORF clone, Homo sapiens(human)

mRNA	Protein	Name
NM_001363665.1	NP_001350594.1	poly(A) polymerase alpha isoform 8
NM_001363664.1	NP_001350593.1	poly(A) polymerase alpha isoform 7
NM_001252007.1	NP_001238936.1	poly(A) polymerase alpha isoform 3
NM_032632.4	NP_116021.2	poly(A) polymerase alpha isoform 1
NM_001293628.1	NP_001280557.1	poly(A) polymerase alpha isoform 5
NM_001363666.1	NP_001350595.1	poly(A) polymerase alpha isoform 9
XM_017020936.1	XP_016876425.1	poly(A) polymerase alpha isoform X4
NM_001363666.2	NP_001350595.1	poly(A) polymerase alpha isoform 9
NM_001293627.1	NP_001280556.1	poly(A) polymerase alpha isoform 4
XM_005267281.1	XP_005267338.1	poly(A) polymerase alpha isoform X1
XM_017020935.1	XP_016876424.1	poly(A) polymerase alpha isoform X2
NM_001363662.1	NP_001350591.1	poly(A) polymerase alpha isoform 6
NM_001363662.2	NP_001350591.1	poly(A) polymerase alpha isoform 6
NM_001363664.2	NP_001350593.1	poly(A) polymerase alpha isoform 7
NM_001293632.2	NP_001280561.1	poly(A) polymerase alpha isoform 5
NM_001363665.2	NP_001350594.1	poly(A) polymerase alpha isoform 8
NM_001252006.1	NP_001238935.1	poly(A) polymerase alpha isoform 2
XM_005267282.2	XP_005267339.1	poly(A) polymerase alpha isoform X3
NM_001293632.1	NP_001280561.1	poly(A) polymerase alpha isoform 5

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu27954	NM_001252006.1 Latest version!	Homo sapiens poly(A) polymerase alpha (PAPOLA), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
OHu27951	NM_001293628.1 Latest version!	Homo sapiens poly(A) polymerase alpha (PAPOLA), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu27951	NM_001293632.2 Latest version!	Homo sapiens poly(A) polymerase alpha (PAPOLA), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu27955	NM_001252007.1 Latest version!	Homo sapiens poly(A) polymerase alpha (PAPOLA), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu27950	NM_032632.4 Latest version!	Homo sapiens poly(A) polymerase alpha (PAPOLA), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$265.30 $379.00
OHu27952	NM_001293627.1 Latest version!	Homo sapiens poly(A) polymerase alpha (PAPOLA), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu47485	NM_001363662.2 Latest version!	Homo sapiens poly(A) polymerase alpha (PAPOLA), transcript variant 7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu47486	NM_001363665.2 Latest version!	Homo sapiens poly(A) polymerase alpha (PAPOLA), transcript variant 9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu47485	XM_005267281.1 Latest version!	Homo sapiens poly(A) polymerase alpha (PAPOLA), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu47486	XM_005267282.2 Latest version!	Homo sapiens poly(A) polymerase alpha (PAPOLA), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu84741	NM_001363664.2 Latest version!	Homo sapiens poly(A) polymerase alpha (PAPOLA), transcript variant 8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu84742	NM_001363666.2 Latest version!	Homo sapiens poly(A) polymerase alpha (PAPOLA), transcript variant 10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu84741	XM_017020935.1 Latest version!	Homo sapiens poly(A) polymerase alpha (PAPOLA), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu84742	XM_017020936.1 Latest version!	Homo sapiens poly(A) polymerase alpha (PAPOLA), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OHu84742	NM_001363666.1	Homo sapiens poly(A) polymerase alpha (PAPOLA), transcript variant 10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu47485	NM_001363662.1	Homo sapiens poly(A) polymerase alpha (PAPOLA), transcript variant 7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu84741	NM_001363664.1	Homo sapiens poly(A) polymerase alpha (PAPOLA), transcript variant 8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu47486	NM_001363665.1	Homo sapiens poly(A) polymerase alpha (PAPOLA), transcript variant 9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu27951	NM_001293632.1	Homo sapiens poly(A) polymerase alpha (PAPOLA), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
Hide Old Accession Versions >>

PAPOLA ( NM_001252006.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001238935.1 Homo sapiens poly(A) polymerase alpha (PAPOLA), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu27954
Clone ID Related Accession (Same CDS sequence)	NM_001252006.1
Accession Version	NM_001252006.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 858bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Homo sapiens(human)
Product	poly(A) polymerase alpha isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB045100.1, BX248753.1, AL163051.5 and BX089817.1. Transcript Variant: This variant (2) lacks multiple exons and its transcription extends past a splice site that is used in variant 1, resulting in a novel 3' coding region and 3' UTR compared to variant 1. It encodes isoform 2 which is shorter and has a distinct C-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.88196.1, SRR1660805.152205.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## regulatory uORF :: PMID: 20174964 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCCGTTTC CAGTTACAAC ACAGGGATCA CAACAAACAC AACCGCCACA GAAGCACTAT 
GGCATTACTT CTCCTATCAG CTTAGCAGCC CCCAAGGAGA CTGACTGCGT ACTTACACAG 
AAACTAATTG AGACATTGAA ACCCTTTGGG GTTTTTGAAG AGGAAGAGGA ACTGCAGCGC 
AGGATTTTAA TTTTGGGAAA ACTAAATAAC CTGGTAAAAG AGTGGATACG AGAAATCAGT 
GAAAGCAAGA ATCTTCCACA ATCTGTAATT GAAAATGTTG GAGGAAAAAT TTTTACATTT 
GGATCTTACA GATTAGGAGT GCATACAAAA GGTGCTGATA TTGATGCGTT GTGTGTTGCA 
CCAAGACATG TTGATCGAAG TGACTTTTTC ACCTCATTCT ATGATAAGTT GAAATTACAG 
GAAGAAGTAA AAGATTTAAG AGCTGTTGAA GAGGCATTCG TACCAGTTAT TAAACTCTGT 
TTTGATGGGA TAGAGATTGA TATTTTGTTT GCAAGATTAG CACTGCAGAC AATTCCTGAA 
GATTTGGATC TACGAGATGA CAGTCTGCTA AAAAATTTAG ATATAAGATG TATAAGAAGT 
CTTAACGGTT GCAGGGTAAC CGATGAAATT TTACATCTAG TACCAAACAT TGACAACTTC 
AGGTTAACTC TGAGAGCTAT CAAACTATGG GCCAAACGCC ACAACATCTA TTCCAATATA 
TTAGGTTTCC TCGGTGGTGT TTCCTGGGCT ATGCTAGTAG CAAGAACTTG CCAGCTTTAT 
CCAAATGCAA TAGCATCAAC TCTTGTACAT AAATTTTTCT TGGTATTTTC TAAATGGTAT 
GTGTTTAGAT TATATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001238935.1
CDS	218..1075
Translation	MPFPVTTQGSQQTQPPQKHYGITSPISLAAPKETDCVLTQKLIETLKPFGVFEEEEELQRRILILGKLNNLVKEWIREISESKNLPQSVIENVGGKIFTFGSYRLGVHTKGADIDALCVAPRHVDRSDFFTSFYDKLKLQEEVKDLRAVEEAFVPVIKLCFDGIEIDILFARLALQTIPEDLDLRDDSLLKNLDIRCIRSLNGCRVTDEILHLVPNIDNFRLTLRAIKLWAKRHNIYSNILGFLGGVSWAMLVARTCQLYPNAIASTLVHKFFLVFSKWYVFRLY

Target ORF information:

RefSeq Version	NM_001252006.1
Organism	Homo sapiens(human)
Definition	Homo sapiens poly(A) polymerase alpha (PAPOLA), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001252006.1

ORF Insert Sequence:

ATGCCGTTTC CAGTTACAAC ACAGGGATCA CAACAAACAC AACCGCCACA GAAGCACTAT 
GGCATTACTT CTCCTATCAG CTTAGCAGCC CCCAAGGAGA CTGACTGCGT ACTTACACAG 
AAACTAATTG AGACATTGAA ACCCTTTGGG GTTTTTGAAG AGGAAGAGGA ACTGCAGCGC 
AGGATTTTAA TTTTGGGAAA ACTAAATAAC CTGGTAAAAG AGTGGATACG AGAAATCAGT 
GAAAGCAAGA ATCTTCCACA ATCTGTAATT GAAAATGTTG GAGGAAAAAT TTTTACATTT 
GGATCTTACA GATTAGGAGT GCATACAAAA GGTGCTGATA TTGATGCGTT GTGTGTTGCA 
CCAAGACATG TTGATCGAAG TGACTTTTTC ACCTCATTCT ATGATAAGTT GAAATTACAG 
GAAGAAGTAA AAGATTTAAG AGCTGTTGAA GAGGCATTCG TACCAGTTAT TAAACTCTGT 
TTTGATGGGA TAGAGATTGA TATTTTGTTT GCAAGATTAG CACTGCAGAC AATTCCTGAA 
GATTTGGATC TACGAGATGA CAGTCTGCTA AAAAATTTAG ATATAAGATG TATAAGAAGT 
CTTAACGGTT GCAGGGTAAC CGATGAAATT TTACATCTAG TACCAAACAT TGACAACTTC 
AGGTTAACTC TGAGAGCTAT CAAACTATGG GCCAAACGCC ACAACATCTA TTCCAATATA 
TTAGGTTTCC TCGGTGGTGT TTCCTGGGCT ATGCTAGTAG CAAGAACTTG CCAGCTTTAT 
CCAAATGCAA TAGCATCAAC TCTTGTACAT AAATTTTTCT TGGTATTTTC TAAATGGTAT 
GTGTTTAGAT TATATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PAPOLA ( NM_001293628.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001280557.1 Homo sapiens poly(A) polymerase alpha (PAPOLA), transcript variant 5, mRNA.

Price & Availability↑

CloneID	OHu27951
Clone ID Related Accession (Same CDS sequence)	NM_001293628.1 , NM_001293632.2 , NM_001293632.1
Accession Version	NM_001293628.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1488bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-05
Organism	Homo sapiens(human)
Product	poly(A) polymerase alpha isoform 5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX248301.1, BC036014.1 and CD655865.1. Transcript Variant: This variant (5) has an alternate 5' terminal exon, which results in a downstream AUG start codon, compared to variant 1. The resulting isoform (5) has a shorter N-terminus, compared to isoform 1. Both variants 5 and 6 encode the same isoform 5. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BX248301.1 [ECO:0000332] ##Evidence-Data-END##

ATGCTAGTAG CAAGAACTTG CCAGCTTTAT CCAAATGCAA TAGCATCAAC TCTTGTACAT 
AAATTTTTCT TGGTATTTTC TAAATGGGAA TGGCCAAATC CAGTGCTATT GAAACAGCCT 
GAAGAATGCA ATCTTAATTT GCCTGTATGG GACCCAAGGG TAAACCCCAG TGATAGGTAC 
CATCTTATGC CTATAATTAC ACCAGCATAC CCACAACAGA ACTCCACGTA CAATGTGTCC 
GTTTCAACAC GGATGGTCAT GGTTGAGGAG TTTAAACAAG GTCTTGCTAT CACAGATGAA 
ATTTTGCTGA GTAAGGCAGA GTGGTCCAAA CTTTTTGAAG CTCCAAACTT CTTTCAAAAG 
TACAAGCATT ATATTGTACT TCTAGCAAGT GCACCAACAG AAAAACAACG CCTGGAATGG 
GTGGGCTTGG TGGAATCAAA AATCCGAATC CTGGTTGGAA GCTTGGAGAA GAATGAATTT 
ATTACACTGG CTCATGTGAA TCCCCAGTCA TTTCCAGCAC CCAAAGAAAA TCCCGACAAG 
GAAGAATTTC GCACGATGTG GGTGATTGGG TTAGTGTTTA AAAAAACAGA AAACTCTGAA 
AACCTCAGTG TTGATCTCAC CTATGATATT CAGTCTTTCA CAGATACAGT TTATAGGCAA 
GCAATAAACA GCAAGATGTT TGAGGTGGAT ATGAAAATTG CTGCAATGCA TGTAAAAAGA 
AAGCAACTCC ATCAACTACT ACCTAATCAT GTGCTTCAGA AAAAGAAAAA GCATTCAACA 
GAAGGTGTCA AATTGACAGC TCTCAATGAC AGCAGCCTCG ACTTGTCTAT GGACAGTGAT 
AACAGCATGT CTGTGCCTTC ACCTACTAGT GCTACGAAGA CCAGTCCATT GAACAGTTCT 
GGCAGCTCTC AGGGCAGAAA CAGTCCTGCT CCAGCTGTAA CAGCAGCATC TGTGACCAAC 
ATACAGGCTA CTGAAGTTTC TGTGCCACAA GTAAATTCCA GTGAAAGCTC AGGGGGTACA 
TCGAGTGAAA GCATTCCTCA AACTGCCACA CAACCAGCCA TTTCTCCACC ACCAAAGCCT 
ACGGTCTCCA GAGTTGTTTC TTCAACACGT CTGGTAAACC CACCACCTAG ATCTTCAGGA 
AATGCAGCAA CTTCAGGAAA TGCAGCAACA AAAATACCTA CTCCTATAGT AGGAGTCAAG 
AGGACATCCT CACCTCATAA AGAAGAGAGT CCCAAGAAAA CCAAAACAGA AGAGGATGAA 
ACAAGTGAAG ATGCTAACTG TCTTGCTTTG AGTGGACATG ATAAAACAGA AGCAAAGGAA 
CAACTTGATA CAGAGACAAG TACAACTCAA TCAGAAACTA TTCAGACAGC GGCTTCTCTG 
TTGGCCTCTC AGAAAACATC CAGTACAGAC CTTTCTGATA TCCCTGCTCT CCCTGCAAAT 
CCTATTCCTG TTATCAAGAA TTCAATAAAA CTGAGATTGA ATCGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001280557.1
CDS	833..2320
Translation	MLVARTCQLYPNAIASTLVHKFFLVFSKWEWPNPVLLKQPEECNLNLPVWDPRVNPSDRYHLMPIITPAYPQQNSTYNVSVSTRMVMVEEFKQGLAITDEILLSKAEWSKLFEAPNFFQKYKHYIVLLASAPTEKQRLEWVGLVESKIRILVGSLEKNEFITLAHVNPQSFPAPKENPDKEEFRTMWVIGLVFKKTENSENLSVDLTYDIQSFTDTVYRQAINSKMFEVDMKIAAMHVKRKQLHQLLPNHVLQKKKKHSTEGVKLTALNDSSLDLSMDSDNSMSVPSPTSATKTSPLNSSGSSQGRNSPAPAVTAASVTNIQATEVSVPQVNSSESSGGTSSESIPQTATQPAISPPPKPTVSRVVSSTRLVNPPPRSSGNAATSGNAATKIPTPIVGVKRTSSPHKEESPKKTKTEEDETSEDANCLALSGHDKTEAKEQLDTETSTTQSETIQTAASLLASQKTSSTDLSDIPALPANPIPVIKNSIKLRLNR

Target ORF information:

RefSeq Version	NM_001293628.1
Organism	Homo sapiens(human)
Definition	Homo sapiens poly(A) polymerase alpha (PAPOLA), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001293628.1

ORF Insert Sequence:

ATGCTAGTAG CAAGAACTTG CCAGCTTTAT CCAAATGCAA TAGCATCAAC TCTTGTACAT 
AAATTTTTCT TGGTATTTTC TAAATGGGAA TGGCCAAATC CAGTGCTATT GAAACAGCCT 
GAAGAATGCA ATCTTAATTT GCCTGTATGG GACCCAAGGG TAAACCCCAG TGATAGGTAC 
CATCTTATGC CTATAATTAC ACCAGCATAC CCACAACAGA ACTCCACGTA CAATGTGTCC 
GTTTCAACAC GGATGGTCAT GGTTGAGGAG TTTAAACAAG GTCTTGCTAT CACAGATGAA 
ATTTTGCTGA GTAAGGCAGA GTGGTCCAAA CTTTTTGAAG CTCCAAACTT CTTTCAAAAG 
TACAAGCATT ATATTGTACT TCTAGCAAGT GCACCAACAG AAAAACAACG CCTGGAATGG 
GTGGGCTTGG TGGAATCAAA AATCCGAATC CTGGTTGGAA GCTTGGAGAA GAATGAATTT 
ATTACACTGG CTCATGTGAA TCCCCAGTCA TTTCCAGCAC CCAAAGAAAA TCCCGACAAG 
GAAGAATTTC GCACGATGTG GGTGATTGGG TTAGTGTTTA AAAAAACAGA AAACTCTGAA 
AACCTCAGTG TTGATCTCAC CTATGATATT CAGTCTTTCA CAGATACAGT TTATAGGCAA 
GCAATAAACA GCAAGATGTT TGAGGTGGAT ATGAAAATTG CTGCAATGCA TGTAAAAAGA 
AAGCAACTCC ATCAACTACT ACCTAATCAT GTGCTTCAGA AAAAGAAAAA GCATTCAACA 
GAAGGTGTCA AATTGACAGC TCTCAATGAC AGCAGCCTCG ACTTGTCTAT GGACAGTGAT 
AACAGCATGT CTGTGCCTTC ACCTACTAGT GCTACGAAGA CCAGTCCATT GAACAGTTCT 
GGCAGCTCTC AGGGCAGAAA CAGTCCTGCT CCAGCTGTAA CAGCAGCATC TGTGACCAAC 
ATACAGGCTA CTGAAGTTTC TGTGCCACAA GTAAATTCCA GTGAAAGCTC AGGGGGTACA 
TCGAGTGAAA GCATTCCTCA AACTGCCACA CAACCAGCCA TTTCTCCACC ACCAAAGCCT 
ACGGTCTCCA GAGTTGTTTC TTCAACACGT CTGGTAAACC CACCACCTAG ATCTTCAGGA 
AATGCAGCAA CTTCAGGAAA TGCAGCAACA AAAATACCTA CTCCTATAGT AGGAGTCAAG 
AGGACATCCT CACCTCATAA AGAAGAGAGT CCCAAGAAAA CCAAAACAGA AGAGGATGAA 
ACAAGTGAAG ATGCTAACTG TCTTGCTTTG AGTGGACATG ATAAAACAGA AGCAAAGGAA 
CAACTTGATA CAGAGACAAG TACAACTCAA TCAGAAACTA TTCAGACAGC GGCTTCTCTG 
TTGGCCTCTC AGAAAACATC CAGTACAGAC CTTTCTGATA TCCCTGCTCT CCCTGCAAAT 
CCTATTCCTG TTATCAAGAA TTCAATAAAA CTGAGATTGA ATCGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PAPOLA ( NM_001293632.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001280561.1 Homo sapiens poly(A) polymerase alpha (PAPOLA), transcript variant 6, mRNA.

Price & Availability↑

CloneID	OHu27951
Clone ID Related Accession (Same CDS sequence)	NM_001293628.1 , NM_001293632.2 , NM_001293632.1
Accession Version	NM_001293632.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1488bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-09
Organism	Homo sapiens(human)
Product	poly(A) polymerase alpha isoform 5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB483858.1, AK307659.1 and BC036014.1. On Jun 2, 2019 this sequence version replaced NM_001293632.1. Transcript Variant: This variant (6) lacks two consecutive exons in the 5' region, which results in a downstream AUG start codon, compared to variant 1. The resulting isoform (5) has a shorter N-terminus, compared to isoform 1. Both variants 5 and 6 encode the same isoform 5. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.228023.1, SRR1803615.220137.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END##

ATGCTAGTAG CAAGAACTTG CCAGCTTTAT CCAAATGCAA TAGCATCAAC TCTTGTACAT 
AAATTTTTCT TGGTATTTTC TAAATGGGAA TGGCCAAATC CAGTGCTATT GAAACAGCCT 
GAAGAATGCA ATCTTAATTT GCCTGTATGG GACCCAAGGG TAAACCCCAG TGATAGGTAC 
CATCTTATGC CTATAATTAC ACCAGCATAC CCACAACAGA ACTCCACGTA CAATGTGTCC 
GTTTCAACAC GGATGGTCAT GGTTGAGGAG TTTAAACAAG GTCTTGCTAT CACAGATGAA 
ATTTTGCTGA GTAAGGCAGA GTGGTCCAAA CTTTTTGAAG CTCCAAACTT CTTTCAAAAG 
TACAAGCATT ATATTGTACT TCTAGCAAGT GCACCAACAG AAAAACAACG CCTGGAATGG 
GTGGGCTTGG TGGAATCAAA AATCCGAATC CTGGTTGGAA GCTTGGAGAA GAATGAATTT 
ATTACACTGG CTCATGTGAA TCCCCAGTCA TTTCCAGCAC CCAAAGAAAA TCCCGACAAG 
GAAGAATTTC GCACGATGTG GGTGATTGGG TTAGTGTTTA AAAAAACAGA AAACTCTGAA 
AACCTCAGTG TTGATCTCAC CTATGATATT CAGTCTTTCA CAGATACAGT TTATAGGCAA 
GCAATAAACA GCAAGATGTT TGAGGTGGAT ATGAAAATTG CTGCAATGCA TGTAAAAAGA 
AAGCAACTCC ATCAACTACT ACCTAATCAT GTGCTTCAGA AAAAGAAAAA GCATTCAACA 
GAAGGTGTCA AATTGACAGC TCTCAATGAC AGCAGCCTCG ACTTGTCTAT GGACAGTGAT 
AACAGCATGT CTGTGCCTTC ACCTACTAGT GCTACGAAGA CCAGTCCATT GAACAGTTCT 
GGCAGCTCTC AGGGCAGAAA CAGTCCTGCT CCAGCTGTAA CAGCAGCATC TGTGACCAAC 
ATACAGGCTA CTGAAGTTTC TGTGCCACAA GTAAATTCCA GTGAAAGCTC AGGGGGTACA 
TCGAGTGAAA GCATTCCTCA AACTGCCACA CAACCAGCCA TTTCTCCACC ACCAAAGCCT 
ACGGTCTCCA GAGTTGTTTC TTCAACACGT CTGGTAAACC CACCACCTAG ATCTTCAGGA 
AATGCAGCAA CTTCAGGAAA TGCAGCAACA AAAATACCTA CTCCTATAGT AGGAGTCAAG 
AGGACATCCT CACCTCATAA AGAAGAGAGT CCCAAGAAAA CCAAAACAGA AGAGGATGAA 
ACAAGTGAAG ATGCTAACTG TCTTGCTTTG AGTGGACATG ATAAAACAGA AGCAAAGGAA 
CAACTTGATA CAGAGACAAG TACAACTCAA TCAGAAACTA TTCAGACAGC GGCTTCTCTG 
TTGGCCTCTC AGAAAACATC CAGTACAGAC CTTTCTGATA TCCCTGCTCT CCCTGCAAAT 
CCTATTCCTG TTATCAAGAA TTCAATAAAA CTGAGATTGA ATCGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001280561.1
CDS	798..2285
Translation	MLVARTCQLYPNAIASTLVHKFFLVFSKWEWPNPVLLKQPEECNLNLPVWDPRVNPSDRYHLMPIITPAYPQQNSTYNVSVSTRMVMVEEFKQGLAITDEILLSKAEWSKLFEAPNFFQKYKHYIVLLASAPTEKQRLEWVGLVESKIRILVGSLEKNEFITLAHVNPQSFPAPKENPDKEEFRTMWVIGLVFKKTENSENLSVDLTYDIQSFTDTVYRQAINSKMFEVDMKIAAMHVKRKQLHQLLPNHVLQKKKKHSTEGVKLTALNDSSLDLSMDSDNSMSVPSPTSATKTSPLNSSGSSQGRNSPAPAVTAASVTNIQATEVSVPQVNSSESSGGTSSESIPQTATQPAISPPPKPTVSRVVSSTRLVNPPPRSSGNAATSGNAATKIPTPIVGVKRTSSPHKEESPKKTKTEEDETSEDANCLALSGHDKTEAKEQLDTETSTTQSETIQTAASLLASQKTSSTDLSDIPALPANPIPVIKNSIKLRLNR

Target ORF information:

RefSeq Version	NM_001293632.2
Organism	Homo sapiens(human)
Definition	Homo sapiens poly(A) polymerase alpha (PAPOLA), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001293632.2

ORF Insert Sequence:

ATGCTAGTAG CAAGAACTTG CCAGCTTTAT CCAAATGCAA TAGCATCAAC TCTTGTACAT 
AAATTTTTCT TGGTATTTTC TAAATGGGAA TGGCCAAATC CAGTGCTATT GAAACAGCCT 
GAAGAATGCA ATCTTAATTT GCCTGTATGG GACCCAAGGG TAAACCCCAG TGATAGGTAC 
CATCTTATGC CTATAATTAC ACCAGCATAC CCACAACAGA ACTCCACGTA CAATGTGTCC 
GTTTCAACAC GGATGGTCAT GGTTGAGGAG TTTAAACAAG GTCTTGCTAT CACAGATGAA 
ATTTTGCTGA GTAAGGCAGA GTGGTCCAAA CTTTTTGAAG CTCCAAACTT CTTTCAAAAG 
TACAAGCATT ATATTGTACT TCTAGCAAGT GCACCAACAG AAAAACAACG CCTGGAATGG 
GTGGGCTTGG TGGAATCAAA AATCCGAATC CTGGTTGGAA GCTTGGAGAA GAATGAATTT 
ATTACACTGG CTCATGTGAA TCCCCAGTCA TTTCCAGCAC CCAAAGAAAA TCCCGACAAG 
GAAGAATTTC GCACGATGTG GGTGATTGGG TTAGTGTTTA AAAAAACAGA AAACTCTGAA 
AACCTCAGTG TTGATCTCAC CTATGATATT CAGTCTTTCA CAGATACAGT TTATAGGCAA 
GCAATAAACA GCAAGATGTT TGAGGTGGAT ATGAAAATTG CTGCAATGCA TGTAAAAAGA 
AAGCAACTCC ATCAACTACT ACCTAATCAT GTGCTTCAGA AAAAGAAAAA GCATTCAACA 
GAAGGTGTCA AATTGACAGC TCTCAATGAC AGCAGCCTCG ACTTGTCTAT GGACAGTGAT 
AACAGCATGT CTGTGCCTTC ACCTACTAGT GCTACGAAGA CCAGTCCATT GAACAGTTCT 
GGCAGCTCTC AGGGCAGAAA CAGTCCTGCT CCAGCTGTAA CAGCAGCATC TGTGACCAAC 
ATACAGGCTA CTGAAGTTTC TGTGCCACAA GTAAATTCCA GTGAAAGCTC AGGGGGTACA 
TCGAGTGAAA GCATTCCTCA AACTGCCACA CAACCAGCCA TTTCTCCACC ACCAAAGCCT 
ACGGTCTCCA GAGTTGTTTC TTCAACACGT CTGGTAAACC CACCACCTAG ATCTTCAGGA 
AATGCAGCAA CTTCAGGAAA TGCAGCAACA AAAATACCTA CTCCTATAGT AGGAGTCAAG 
AGGACATCCT CACCTCATAA AGAAGAGAGT CCCAAGAAAA CCAAAACAGA AGAGGATGAA 
ACAAGTGAAG ATGCTAACTG TCTTGCTTTG AGTGGACATG ATAAAACAGA AGCAAAGGAA 
CAACTTGATA CAGAGACAAG TACAACTCAA TCAGAAACTA TTCAGACAGC GGCTTCTCTG 
TTGGCCTCTC AGAAAACATC CAGTACAGAC CTTTCTGATA TCCCTGCTCT CCCTGCAAAT 
CCTATTCCTG TTATCAAGAA TTCAATAAAA CTGAGATTGA ATCGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PAPOLA ( NM_001293632.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001280561.1 Homo sapiens poly(A) polymerase alpha (PAPOLA), transcript variant 6, mRNA.

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CloneID	OHu27951
Clone ID Related Accession (Same CDS sequence)	NM_001293628.1 , NM_001293632.2 , NM_001293632.1
Accession Version	NM_001293632.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1488bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-29
Organism	Homo sapiens(human)
Product	poly(A) polymerase alpha isoform 5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB483858.1, AK307659.1, BC036014.1 and CD655865.1. Transcript Variant: This variant (6) lacks two consecutive exons in the 5' region, which results in a downstream AUG start codon, compared to variant 1. The resulting isoform (5) has a shorter N-terminus, compared to isoform 1. Both variants 5 and 6 encode the same isoform 5. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.220137.1, SRR1660807.228023.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END##

ATGCTAGTAG CAAGAACTTG CCAGCTTTAT CCAAATGCAA TAGCATCAAC TCTTGTACAT 
AAATTTTTCT TGGTATTTTC TAAATGGGAA TGGCCAAATC CAGTGCTATT GAAACAGCCT 
GAAGAATGCA ATCTTAATTT GCCTGTATGG GACCCAAGGG TAAACCCCAG TGATAGGTAC 
CATCTTATGC CTATAATTAC ACCAGCATAC CCACAACAGA ACTCCACGTA CAATGTGTCC 
GTTTCAACAC GGATGGTCAT GGTTGAGGAG TTTAAACAAG GTCTTGCTAT CACAGATGAA 
ATTTTGCTGA GTAAGGCAGA GTGGTCCAAA CTTTTTGAAG CTCCAAACTT CTTTCAAAAG 
TACAAGCATT ATATTGTACT TCTAGCAAGT GCACCAACAG AAAAACAACG CCTGGAATGG 
GTGGGCTTGG TGGAATCAAA AATCCGAATC CTGGTTGGAA GCTTGGAGAA GAATGAATTT 
ATTACACTGG CTCATGTGAA TCCCCAGTCA TTTCCAGCAC CCAAAGAAAA TCCCGACAAG 
GAAGAATTTC GCACGATGTG GGTGATTGGG TTAGTGTTTA AAAAAACAGA AAACTCTGAA 
AACCTCAGTG TTGATCTCAC CTATGATATT CAGTCTTTCA CAGATACAGT TTATAGGCAA 
GCAATAAACA GCAAGATGTT TGAGGTGGAT ATGAAAATTG CTGCAATGCA TGTAAAAAGA 
AAGCAACTCC ATCAACTACT ACCTAATCAT GTGCTTCAGA AAAAGAAAAA GCATTCAACA 
GAAGGTGTCA AATTGACAGC TCTCAATGAC AGCAGCCTCG ACTTGTCTAT GGACAGTGAT 
AACAGCATGT CTGTGCCTTC ACCTACTAGT GCTACGAAGA CCAGTCCATT GAACAGTTCT 
GGCAGCTCTC AGGGCAGAAA CAGTCCTGCT CCAGCTGTAA CAGCAGCATC TGTGACCAAC 
ATACAGGCTA CTGAAGTTTC TGTGCCACAA GTAAATTCCA GTGAAAGCTC AGGGGGTACA 
TCGAGTGAAA GCATTCCTCA AACTGCCACA CAACCAGCCA TTTCTCCACC ACCAAAGCCT 
ACGGTCTCCA GAGTTGTTTC TTCAACACGT CTGGTAAACC CACCACCTAG ATCTTCAGGA 
AATGCAGCAA CTTCAGGAAA TGCAGCAACA AAAATACCTA CTCCTATAGT AGGAGTCAAG 
AGGACATCCT CACCTCATAA AGAAGAGAGT CCCAAGAAAA CCAAAACAGA AGAGGATGAA 
ACAAGTGAAG ATGCTAACTG TCTTGCTTTG AGTGGACATG ATAAAACAGA AGCAAAGGAA 
CAACTTGATA CAGAGACAAG TACAACTCAA TCAGAAACTA TTCAGACAGC GGCTTCTCTG 
TTGGCCTCTC AGAAAACATC CAGTACAGAC CTTTCTGATA TCCCTGCTCT CCCTGCAAAT 
CCTATTCCTG TTATCAAGAA TTCAATAAAA CTGAGATTGA ATCGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001280561.1
CDS	804..2291
Translation	MLVARTCQLYPNAIASTLVHKFFLVFSKWEWPNPVLLKQPEECNLNLPVWDPRVNPSDRYHLMPIITPAYPQQNSTYNVSVSTRMVMVEEFKQGLAITDEILLSKAEWSKLFEAPNFFQKYKHYIVLLASAPTEKQRLEWVGLVESKIRILVGSLEKNEFITLAHVNPQSFPAPKENPDKEEFRTMWVIGLVFKKTENSENLSVDLTYDIQSFTDTVYRQAINSKMFEVDMKIAAMHVKRKQLHQLLPNHVLQKKKKHSTEGVKLTALNDSSLDLSMDSDNSMSVPSPTSATKTSPLNSSGSSQGRNSPAPAVTAASVTNIQATEVSVPQVNSSESSGGTSSESIPQTATQPAISPPPKPTVSRVVSSTRLVNPPPRSSGNAATSGNAATKIPTPIVGVKRTSSPHKEESPKKTKTEEDETSEDANCLALSGHDKTEAKEQLDTETSTTQSETIQTAASLLASQKTSSTDLSDIPALPANPIPVIKNSIKLRLNR

Target ORF information:

RefSeq Version	NM_001293632.1
Organism	Homo sapiens(human)
Definition	Homo sapiens poly(A) polymerase alpha (PAPOLA), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001293632.1

ORF Insert Sequence:

ATGCTAGTAG CAAGAACTTG CCAGCTTTAT CCAAATGCAA TAGCATCAAC TCTTGTACAT 
AAATTTTTCT TGGTATTTTC TAAATGGGAA TGGCCAAATC CAGTGCTATT GAAACAGCCT 
GAAGAATGCA ATCTTAATTT GCCTGTATGG GACCCAAGGG TAAACCCCAG TGATAGGTAC 
CATCTTATGC CTATAATTAC ACCAGCATAC CCACAACAGA ACTCCACGTA CAATGTGTCC 
GTTTCAACAC GGATGGTCAT GGTTGAGGAG TTTAAACAAG GTCTTGCTAT CACAGATGAA 
ATTTTGCTGA GTAAGGCAGA GTGGTCCAAA CTTTTTGAAG CTCCAAACTT CTTTCAAAAG 
TACAAGCATT ATATTGTACT TCTAGCAAGT GCACCAACAG AAAAACAACG CCTGGAATGG 
GTGGGCTTGG TGGAATCAAA AATCCGAATC CTGGTTGGAA GCTTGGAGAA GAATGAATTT 
ATTACACTGG CTCATGTGAA TCCCCAGTCA TTTCCAGCAC CCAAAGAAAA TCCCGACAAG 
GAAGAATTTC GCACGATGTG GGTGATTGGG TTAGTGTTTA AAAAAACAGA AAACTCTGAA 
AACCTCAGTG TTGATCTCAC CTATGATATT CAGTCTTTCA CAGATACAGT TTATAGGCAA 
GCAATAAACA GCAAGATGTT TGAGGTGGAT ATGAAAATTG CTGCAATGCA TGTAAAAAGA 
AAGCAACTCC ATCAACTACT ACCTAATCAT GTGCTTCAGA AAAAGAAAAA GCATTCAACA 
GAAGGTGTCA AATTGACAGC TCTCAATGAC AGCAGCCTCG ACTTGTCTAT GGACAGTGAT 
AACAGCATGT CTGTGCCTTC ACCTACTAGT GCTACGAAGA CCAGTCCATT GAACAGTTCT 
GGCAGCTCTC AGGGCAGAAA CAGTCCTGCT CCAGCTGTAA CAGCAGCATC TGTGACCAAC 
ATACAGGCTA CTGAAGTTTC TGTGCCACAA GTAAATTCCA GTGAAAGCTC AGGGGGTACA 
TCGAGTGAAA GCATTCCTCA AACTGCCACA CAACCAGCCA TTTCTCCACC ACCAAAGCCT 
ACGGTCTCCA GAGTTGTTTC TTCAACACGT CTGGTAAACC CACCACCTAG ATCTTCAGGA 
AATGCAGCAA CTTCAGGAAA TGCAGCAACA AAAATACCTA CTCCTATAGT AGGAGTCAAG 
AGGACATCCT CACCTCATAA AGAAGAGAGT CCCAAGAAAA CCAAAACAGA AGAGGATGAA 
ACAAGTGAAG ATGCTAACTG TCTTGCTTTG AGTGGACATG ATAAAACAGA AGCAAAGGAA 
CAACTTGATA CAGAGACAAG TACAACTCAA TCAGAAACTA TTCAGACAGC GGCTTCTCTG 
TTGGCCTCTC AGAAAACATC CAGTACAGAC CTTTCTGATA TCCCTGCTCT CCCTGCAAAT 
CCTATTCCTG TTATCAAGAA TTCAATAAAA CTGAGATTGA ATCGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PAPOLA ( NM_001252007.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001238936.1 Homo sapiens poly(A) polymerase alpha (PAPOLA), transcript variant 3, mRNA.

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CloneID	OHu27955
Clone ID Related Accession (Same CDS sequence)	NM_001252007.1
Accession Version	NM_001252007.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 717bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-05
Organism	Homo sapiens(human)
Product	poly(A) polymerase alpha isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB045100.1, BX161482.1, AL163051.5 and BX089817.1. Transcript Variant: This variant (3) lacks multiple exons and its transcription extends past a splice site that is used in variant 1, resulting in a novel 3' coding region and 3' UTR compared to variant 1. It encodes isoform 3 which is shorter and has a distinct C-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.227853.1, SRR1660809.253388.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## regulatory uORF :: PMID: 20174964 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCCGTTTC CAGTTACAAC ACAGGGATCA CAACAAACAC AACCGCCACA GAAGCACTAT 
GGCATTACTT CTCCTATCAG CTTAGCAGCC CCCAAGGAGA CTGACTGCGT ACTTACACAG 
AAACTAATTG AGACATTGAA ACCCTTTGGG GTTTTTGAAG AGGAAGAGGA ACTGCAGCGC 
AGGATTTTAA TTTTGGGAAA ACTAAATAAC CTGGTAAAAG AGTGGATACG AGAAATCAGT 
GAAAGCAAGA ATCTTCCACA ATCTGTAATT GAAAATGTTG GAGGAAAAAT TTTTACATTT 
GGATCTTACA GATTAGGAGT GCATACAAAA GGTGCTGATA TTGATGCGTT GTGTGTTGCA 
CCAAGACATG TTGATCGAAG TGACTTTTTC ACCTCATTCT ATGATAAGTT GAAATTACAG 
GAAGAAGTAA AAGATTTAAG AGCTGTTGAA GAGGCATTCG TACCAGTTAT TAAACTCTGT 
TTTGATGGGA TAGAGATTGA TATTTTGTTT GCAAGATTAG CACTGCAGAC AATTCCTGAA 
GATTTGGATC TACGAGATGA CAGTCTGCTA AAAAATTTAG ATATAAGATG TATAAGAAGT 
CTTAACGGTA TGAGAAAGCC TACTTCCTTT TGTGTACTTC AGTTTTTGTC AGATATTAGT 
TGTTTTTACA CAAGTTTTGT ATTGAAGCTT TTTATAGCTA TATTGTTAAC ACAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001238936.1
CDS	218..934
Translation	MPFPVTTQGSQQTQPPQKHYGITSPISLAAPKETDCVLTQKLIETLKPFGVFEEEEELQRRILILGKLNNLVKEWIREISESKNLPQSVIENVGGKIFTFGSYRLGVHTKGADIDALCVAPRHVDRSDFFTSFYDKLKLQEEVKDLRAVEEAFVPVIKLCFDGIEIDILFARLALQTIPEDLDLRDDSLLKNLDIRCIRSLNGMRKPTSFCVLQFLSDISCFYTSFVLKLFIAILLTQ

Target ORF information:

RefSeq Version	NM_001252007.1
Organism	Homo sapiens(human)
Definition	Homo sapiens poly(A) polymerase alpha (PAPOLA), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001252007.1

ORF Insert Sequence:

ATGCCGTTTC CAGTTACAAC ACAGGGATCA CAACAAACAC AACCGCCACA GAAGCACTAT 
GGCATTACTT CTCCTATCAG CTTAGCAGCC CCCAAGGAGA CTGACTGCGT ACTTACACAG 
AAACTAATTG AGACATTGAA ACCCTTTGGG GTTTTTGAAG AGGAAGAGGA ACTGCAGCGC 
AGGATTTTAA TTTTGGGAAA ACTAAATAAC CTGGTAAAAG AGTGGATACG AGAAATCAGT 
GAAAGCAAGA ATCTTCCACA ATCTGTAATT GAAAATGTTG GAGGAAAAAT TTTTACATTT 
GGATCTTACA GATTAGGAGT GCATACAAAA GGTGCTGATA TTGATGCGTT GTGTGTTGCA 
CCAAGACATG TTGATCGAAG TGACTTTTTC ACCTCATTCT ATGATAAGTT GAAATTACAG 
GAAGAAGTAA AAGATTTAAG AGCTGTTGAA GAGGCATTCG TACCAGTTAT TAAACTCTGT 
TTTGATGGGA TAGAGATTGA TATTTTGTTT GCAAGATTAG CACTGCAGAC AATTCCTGAA 
GATTTGGATC TACGAGATGA CAGTCTGCTA AAAAATTTAG ATATAAGATG TATAAGAAGT 
CTTAACGGTA TGAGAAAGCC TACTTCCTTT TGTGTACTTC AGTTTTTGTC AGATATTAGT 
TGTTTTTACA CAAGTTTTGT ATTGAAGCTT TTTATAGCTA TATTGTTAAC ACAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PAPOLA ( NM_032632.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_116021.2 Homo sapiens poly(A) polymerase alpha (PAPOLA), transcript variant 1, mRNA.

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CloneID	OHu27950
Clone ID Related Accession (Same CDS sequence)	NM_032632.4
Accession Version	NM_032632.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2238bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-05
Organism	Homo sapiens(human)
Product	poly(A) polymerase alpha isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB045100.1, BC036014.1 and AA236200.1. This sequence is a reference standard in the RefSeqGene project. On Oct 28, 2011 this sequence version replaced NM_032632.3. Transcript Variant: This variant (1) encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC036014.1, SRR1660809.262773.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein regulatory uORF :: PMID: 20174964 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCCGTTTC CAGTTACAAC ACAGGGATCA CAACAAACAC AACCGCCACA GAAGCACTAT 
GGCATTACTT CTCCTATCAG CTTAGCAGCC CCCAAGGAGA CTGACTGCGT ACTTACACAG 
AAACTAATTG AGACATTGAA ACCCTTTGGG GTTTTTGAAG AGGAAGAGGA ACTGCAGCGC 
AGGATTTTAA TTTTGGGAAA ACTAAATAAC CTGGTAAAAG AGTGGATACG AGAAATCAGT 
GAAAGCAAGA ATCTTCCACA ATCTGTAATT GAAAATGTTG GAGGAAAAAT TTTTACATTT 
GGATCTTACA GATTAGGAGT GCATACAAAA GGTGCTGATA TTGATGCGTT GTGTGTTGCA 
CCAAGACATG TTGATCGAAG TGACTTTTTC ACCTCATTCT ATGATAAGTT GAAATTACAG 
GAAGAAGTAA AAGATTTAAG AGCTGTTGAA GAGGCATTCG TACCAGTTAT TAAACTCTGT 
TTTGATGGGA TAGAGATTGA TATTTTGTTT GCAAGATTAG CACTGCAGAC AATTCCTGAA 
GATTTGGATC TACGAGATGA CAGTCTGCTA AAAAATTTAG ATATAAGATG TATAAGAAGT 
CTTAACGGTT GCAGGGTAAC CGATGAAATT TTACATCTAG TACCAAACAT TGACAACTTC 
AGGTTAACTC TGAGAGCTAT CAAACTATGG GCCAAACGCC ACAACATCTA TTCCAATATA 
TTAGGTTTCC TCGGTGGTGT TTCCTGGGCT ATGCTAGTAG CAAGAACTTG CCAGCTTTAT 
CCAAATGCAA TAGCATCAAC TCTTGTACAT AAATTTTTCT TGGTATTTTC TAAATGGGAA 
TGGCCAAATC CAGTGCTATT GAAACAGCCT GAAGAATGCA ATCTTAATTT GCCTGTATGG 
GACCCAAGGG TAAACCCCAG TGATAGGTAC CATCTTATGC CTATAATTAC ACCAGCATAC 
CCACAACAGA ACTCCACGTA CAATGTGTCC GTTTCAACAC GGATGGTCAT GGTTGAGGAG 
TTTAAACAAG GTCTTGCTAT CACAGATGAA ATTTTGCTGA GTAAGGCAGA GTGGTCCAAA 
CTTTTTGAAG CTCCAAACTT CTTTCAAAAG TACAAGCATT ATATTGTACT TCTAGCAAGT 
GCACCAACAG AAAAACAACG CCTGGAATGG GTGGGCTTGG TGGAATCAAA AATCCGAATC 
CTGGTTGGAA GCTTGGAGAA GAATGAATTT ATTACACTGG CTCATGTGAA TCCCCAGTCA 
TTTCCAGCAC CCAAAGAAAA TCCCGACAAG GAAGAATTTC GCACGATGTG GGTGATTGGG 
TTAGTGTTTA AAAAAACAGA AAACTCTGAA AACCTCAGTG TTGATCTCAC CTATGATATT 
CAGTCTTTCA CAGATACAGT TTATAGGCAA GCAATAAACA GCAAGATGTT TGAGGTGGAT 
ATGAAAATTG CTGCAATGCA TGTAAAAAGA AAGCAACTCC ATCAACTACT ACCTAATCAT 
GTGCTTCAGA AAAAGAAAAA GCATTCAACA GAAGGTGTCA AATTGACAGC TCTCAATGAC 
AGCAGCCTCG ACTTGTCTAT GGACAGTGAT AACAGCATGT CTGTGCCTTC ACCTACTAGT 
GCTACGAAGA CCAGTCCATT GAACAGTTCT GGCAGCTCTC AGGGCAGAAA CAGTCCTGCT 
CCAGCTGTAA CAGCAGCATC TGTGACCAAC ATACAGGCTA CTGAAGTTTC TGTGCCACAA 
GTAAATTCCA GTGAAAGCTC AGGGGGTACA TCGAGTGAAA GCATTCCTCA AACTGCCACA 
CAACCAGCCA TTTCTCCACC ACCAAAGCCT ACGGTCTCCA GAGTTGTTTC TTCAACACGT 
CTGGTAAACC CACCACCTAG ATCTTCAGGA AATGCAGCAA CTTCAGGAAA TGCAGCAACA 
AAAATACCTA CTCCTATAGT AGGAGTCAAG AGGACATCCT CACCTCATAA AGAAGAGAGT 
CCCAAGAAAA CCAAAACAGA AGAGGATGAA ACAAGTGAAG ATGCTAACTG TCTTGCTTTG 
AGTGGACATG ATAAAACAGA AGCAAAGGAA CAACTTGATA CAGAGACAAG TACAACTCAA 
TCAGAAACTA TTCAGACAGC GGCTTCTCTG TTGGCCTCTC AGAAAACATC CAGTACAGAC 
CTTTCTGATA TCCCTGCTCT CCCTGCAAAT CCTATTCCTG TTATCAAGAA TTCAATAAAA 
CTGAGATTGA ATCGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_116021.2
CDS	218..2455
Translation	MPFPVTTQGSQQTQPPQKHYGITSPISLAAPKETDCVLTQKLIETLKPFGVFEEEEELQRRILILGKLNNLVKEWIREISESKNLPQSVIENVGGKIFTFGSYRLGVHTKGADIDALCVAPRHVDRSDFFTSFYDKLKLQEEVKDLRAVEEAFVPVIKLCFDGIEIDILFARLALQTIPEDLDLRDDSLLKNLDIRCIRSLNGCRVTDEILHLVPNIDNFRLTLRAIKLWAKRHNIYSNILGFLGGVSWAMLVARTCQLYPNAIASTLVHKFFLVFSKWEWPNPVLLKQPEECNLNLPVWDPRVNPSDRYHLMPIITPAYPQQNSTYNVSVSTRMVMVEEFKQGLAITDEILLSKAEWSKLFEAPNFFQKYKHYIVLLASAPTEKQRLEWVGLVESKIRILVGSLEKNEFITLAHVNPQSFPAPKENPDKEEFRTMWVIGLVFKKTENSENLSVDLTYDIQSFTDTVYRQAINSKMFEVDMKIAAMHVKRKQLHQLLPNHVLQKKKKHSTEGVKLTALNDSSLDLSMDSDNSMSVPSPTSATKTSPLNSSGSSQGRNSPAPAVTAASVTNIQATEVSVPQVNSSESSGGTSSESIPQTATQPAISPPPKPTVSRVVSSTRLVNPPPRSSGNAATSGNAATKIPTPIVGVKRTSSPHKEESPKKTKTEEDETSEDANCLALSGHDKTEAKEQLDTETSTTQSETIQTAASLLASQKTSSTDLSDIPALPANPIPVIKNSIKLRLNR

Target ORF information:

RefSeq Version	NM_032632.4
Organism	Homo sapiens(human)
Definition	Homo sapiens poly(A) polymerase alpha (PAPOLA), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_032632.4

ORF Insert Sequence:

ATGCCGTTTC CAGTTACAAC ACAGGGATCA CAACAAACAC AACCGCCACA GAAGCACTAT 
GGCATTACTT CTCCTATCAG CTTAGCAGCC CCCAAGGAGA CTGACTGCGT ACTTACACAG 
AAACTAATTG AGACATTGAA ACCCTTTGGG GTTTTTGAAG AGGAAGAGGA ACTGCAGCGC 
AGGATTTTAA TTTTGGGAAA ACTAAATAAC CTGGTAAAAG AGTGGATACG AGAAATCAGT 
GAAAGCAAGA ATCTTCCACA ATCTGTAATT GAAAATGTTG GAGGAAAAAT TTTTACATTT 
GGATCTTACA GATTAGGAGT GCATACAAAA GGTGCTGATA TTGATGCGTT GTGTGTTGCA 
CCAAGACATG TTGATCGAAG TGACTTTTTC ACCTCATTCT ATGATAAGTT GAAATTACAG 
GAAGAAGTAA AAGATTTAAG AGCTGTTGAA GAGGCATTCG TACCAGTTAT TAAACTCTGT 
TTTGATGGGA TAGAGATTGA TATTTTGTTT GCAAGATTAG CACTGCAGAC AATTCCTGAA 
GATTTGGATC TACGAGATGA CAGTCTGCTA AAAAATTTAG ATATAAGATG TATAAGAAGT 
CTTAACGGTT GCAGGGTAAC CGATGAAATT TTACATCTAG TACCAAACAT TGACAACTTC 
AGGTTAACTC TGAGAGCTAT CAAACTATGG GCCAAACGCC ACAACATCTA TTCCAATATA 
TTAGGTTTCC TCGGTGGTGT TTCCTGGGCT ATGCTAGTAG CAAGAACTTG CCAGCTTTAT 
CCAAATGCAA TAGCATCAAC TCTTGTACAT AAATTTTTCT TGGTATTTTC TAAATGGGAA 
TGGCCAAATC CAGTGCTATT GAAACAGCCT GAAGAATGCA ATCTTAATTT GCCTGTATGG 
GACCCAAGGG TAAACCCCAG TGATAGGTAC CATCTTATGC CTATAATTAC ACCAGCATAC 
CCACAACAGA ACTCCACGTA CAATGTGTCC GTTTCAACAC GGATGGTCAT GGTTGAGGAG 
TTTAAACAAG GTCTTGCTAT CACAGATGAA ATTTTGCTGA GTAAGGCAGA GTGGTCCAAA 
CTTTTTGAAG CTCCAAACTT CTTTCAAAAG TACAAGCATT ATATTGTACT TCTAGCAAGT 
GCACCAACAG AAAAACAACG CCTGGAATGG GTGGGCTTGG TGGAATCAAA AATCCGAATC 
CTGGTTGGAA GCTTGGAGAA GAATGAATTT ATTACACTGG CTCATGTGAA TCCCCAGTCA 
TTTCCAGCAC CCAAAGAAAA TCCCGACAAG GAAGAATTTC GCACGATGTG GGTGATTGGG 
TTAGTGTTTA AAAAAACAGA AAACTCTGAA AACCTCAGTG TTGATCTCAC CTATGATATT 
CAGTCTTTCA CAGATACAGT TTATAGGCAA GCAATAAACA GCAAGATGTT TGAGGTGGAT 
ATGAAAATTG CTGCAATGCA TGTAAAAAGA AAGCAACTCC ATCAACTACT ACCTAATCAT 
GTGCTTCAGA AAAAGAAAAA GCATTCAACA GAAGGTGTCA AATTGACAGC TCTCAATGAC 
AGCAGCCTCG ACTTGTCTAT GGACAGTGAT AACAGCATGT CTGTGCCTTC ACCTACTAGT 
GCTACGAAGA CCAGTCCATT GAACAGTTCT GGCAGCTCTC AGGGCAGAAA CAGTCCTGCT 
CCAGCTGTAA CAGCAGCATC TGTGACCAAC ATACAGGCTA CTGAAGTTTC TGTGCCACAA 
GTAAATTCCA GTGAAAGCTC AGGGGGTACA TCGAGTGAAA GCATTCCTCA AACTGCCACA 
CAACCAGCCA TTTCTCCACC ACCAAAGCCT ACGGTCTCCA GAGTTGTTTC TTCAACACGT 
CTGGTAAACC CACCACCTAG ATCTTCAGGA AATGCAGCAA CTTCAGGAAA TGCAGCAACA 
AAAATACCTA CTCCTATAGT AGGAGTCAAG AGGACATCCT CACCTCATAA AGAAGAGAGT 
CCCAAGAAAA CCAAAACAGA AGAGGATGAA ACAAGTGAAG ATGCTAACTG TCTTGCTTTG 
AGTGGACATG ATAAAACAGA AGCAAAGGAA CAACTTGATA CAGAGACAAG TACAACTCAA 
TCAGAAACTA TTCAGACAGC GGCTTCTCTG TTGGCCTCTC AGAAAACATC CAGTACAGAC 
CTTTCTGATA TCCCTGCTCT CCCTGCAAAT CCTATTCCTG TTATCAAGAA TTCAATAAAA 
CTGAGATTGA ATCGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PAPOLA ( NM_001293627.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001280556.1 Homo sapiens poly(A) polymerase alpha (PAPOLA), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu27952
Clone ID Related Accession (Same CDS sequence)	NM_001293627.1
Accession Version	NM_001293627.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2235bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-05
Organism	Homo sapiens(human)
Product	poly(A) polymerase alpha isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB483858.1, BC015150.2, BC036014.1 and CD655865.1. On Jun 11, 2014 this sequence version replaced XM_005267280.1. Transcript Variant: This variant (4) has an alternate splice site in the 3' coding region, compared to variant 1. The resulting isoform (4) lacks an internal aa, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.96105.1, BC015150.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## regulatory uORF :: PMID: 20174964 ##RefSeq-Attributes-END##

ATGCCGTTTC CAGTTACAAC ACAGGGATCA CAACAAACAC AACCGCCACA GAAGCACTAT 
GGCATTACTT CTCCTATCAG CTTAGCAGCC CCCAAGGAGA CTGACTGCGT ACTTACACAG 
AAACTAATTG AGACATTGAA ACCCTTTGGG GTTTTTGAAG AGGAAGAGGA ACTGCAGCGC 
AGGATTTTAA TTTTGGGAAA ACTAAATAAC CTGGTAAAAG AGTGGATACG AGAAATCAGT 
GAAAGCAAGA ATCTTCCACA ATCTGTAATT GAAAATGTTG GAGGAAAAAT TTTTACATTT 
GGATCTTACA GATTAGGAGT GCATACAAAA GGTGCTGATA TTGATGCGTT GTGTGTTGCA 
CCAAGACATG TTGATCGAAG TGACTTTTTC ACCTCATTCT ATGATAAGTT GAAATTACAG 
GAAGAAGTAA AAGATTTAAG AGCTGTTGAA GAGGCATTCG TACCAGTTAT TAAACTCTGT 
TTTGATGGGA TAGAGATTGA TATTTTGTTT GCAAGATTAG CACTGCAGAC AATTCCTGAA 
GATTTGGATC TACGAGATGA CAGTCTGCTA AAAAATTTAG ATATAAGATG TATAAGAAGT 
CTTAACGGTT GCAGGGTAAC CGATGAAATT TTACATCTAG TACCAAACAT TGACAACTTC 
AGGTTAACTC TGAGAGCTAT CAAACTATGG GCCAAACGCC ACAACATCTA TTCCAATATA 
TTAGGTTTCC TCGGTGGTGT TTCCTGGGCT ATGCTAGTAG CAAGAACTTG CCAGCTTTAT 
CCAAATGCAA TAGCATCAAC TCTTGTACAT AAATTTTTCT TGGTATTTTC TAAATGGGAA 
TGGCCAAATC CAGTGCTATT GAAACAGCCT GAAGAATGCA ATCTTAATTT GCCTGTATGG 
GACCCAAGGG TAAACCCCAG TGATAGGTAC CATCTTATGC CTATAATTAC ACCAGCATAC 
CCACAACAGA ACTCCACGTA CAATGTGTCC GTTTCAACAC GGATGGTCAT GGTTGAGGAG 
TTTAAACAAG GTCTTGCTAT CACAGATGAA ATTTTGCTGA GTAAGGCAGA GTGGTCCAAA 
CTTTTTGAAG CTCCAAACTT CTTTCAAAAG TACAAGCATT ATATTGTACT TCTAGCAAGT 
GCACCAACAG AAAAACAACG CCTGGAATGG GTGGGCTTGG TGGAATCAAA AATCCGAATC 
CTGGTTGGAA GCTTGGAGAA GAATGAATTT ATTACACTGG CTCATGTGAA TCCCCAGTCA 
TTTCCAGCAC CCAAAGAAAA TCCCGACAAG GAAGAATTTC GCACGATGTG GGTGATTGGG 
TTAGTGTTTA AAAAAACAGA AAACTCTGAA AACCTCAGTG TTGATCTCAC CTATGATATT 
CAGTCTTTCA CAGATACAGT TTATAGGCAA GCAATAAACA GCAAGATGTT TGAGGTGGAT 
ATGAAAATTG CTGCAATGCA TGTAAAAAGA AAGCAACTCC ATCAACTACT ACCTAATCAT 
GTGCTTCAGA AAAAGAAAAA GCATTCAACA GAAGGTGTCA AATTGACAGC TCTCAATGAC 
AGCAGCCTCG ACTTGTCTAT GGACAGTGAT AACAGCATGT CTGTGCCTTC ACCTACTAGT 
GCTACGAAGA CCAGTCCATT GAACAGTTCT GGCAGCTCTC AGGGAAACAG TCCTGCTCCA 
GCTGTAACAG CAGCATCTGT GACCAACATA CAGGCTACTG AAGTTTCTGT GCCACAAGTA 
AATTCCAGTG AAAGCTCAGG GGGTACATCG AGTGAAAGCA TTCCTCAAAC TGCCACACAA 
CCAGCCATTT CTCCACCACC AAAGCCTACG GTCTCCAGAG TTGTTTCTTC AACACGTCTG 
GTAAACCCAC CACCTAGATC TTCAGGAAAT GCAGCAACTT CAGGAAATGC AGCAACAAAA 
ATACCTACTC CTATAGTAGG AGTCAAGAGG ACATCCTCAC CTCATAAAGA AGAGAGTCCC 
AAGAAAACCA AAACAGAAGA GGATGAAACA AGTGAAGATG CTAACTGTCT TGCTTTGAGT 
GGACATGATA AAACAGAAGC AAAGGAACAA CTTGATACAG AGACAAGTAC AACTCAATCA 
GAAACTATTC AGACAGCGGC TTCTCTGTTG GCCTCTCAGA AAACATCCAG TACAGACCTT 
TCTGATATCC CTGCTCTCCC TGCAAATCCT ATTCCTGTTA TCAAGAATTC AATAAAACTG 
AGATTGAATC GGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001280556.1
CDS	218..2452
Translation	MPFPVTTQGSQQTQPPQKHYGITSPISLAAPKETDCVLTQKLIETLKPFGVFEEEEELQRRILILGKLNNLVKEWIREISESKNLPQSVIENVGGKIFTFGSYRLGVHTKGADIDALCVAPRHVDRSDFFTSFYDKLKLQEEVKDLRAVEEAFVPVIKLCFDGIEIDILFARLALQTIPEDLDLRDDSLLKNLDIRCIRSLNGCRVTDEILHLVPNIDNFRLTLRAIKLWAKRHNIYSNILGFLGGVSWAMLVARTCQLYPNAIASTLVHKFFLVFSKWEWPNPVLLKQPEECNLNLPVWDPRVNPSDRYHLMPIITPAYPQQNSTYNVSVSTRMVMVEEFKQGLAITDEILLSKAEWSKLFEAPNFFQKYKHYIVLLASAPTEKQRLEWVGLVESKIRILVGSLEKNEFITLAHVNPQSFPAPKENPDKEEFRTMWVIGLVFKKTENSENLSVDLTYDIQSFTDTVYRQAINSKMFEVDMKIAAMHVKRKQLHQLLPNHVLQKKKKHSTEGVKLTALNDSSLDLSMDSDNSMSVPSPTSATKTSPLNSSGSSQGNSPAPAVTAASVTNIQATEVSVPQVNSSESSGGTSSESIPQTATQPAISPPPKPTVSRVVSSTRLVNPPPRSSGNAATSGNAATKIPTPIVGVKRTSSPHKEESPKKTKTEEDETSEDANCLALSGHDKTEAKEQLDTETSTTQSETIQTAASLLASQKTSSTDLSDIPALPANPIPVIKNSIKLRLNR

Target ORF information:

RefSeq Version	NM_001293627.1
Organism	Homo sapiens(human)
Definition	Homo sapiens poly(A) polymerase alpha (PAPOLA), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001293627.1

ORF Insert Sequence:

ATGCCGTTTC CAGTTACAAC ACAGGGATCA CAACAAACAC AACCGCCACA GAAGCACTAT 
GGCATTACTT CTCCTATCAG CTTAGCAGCC CCCAAGGAGA CTGACTGCGT ACTTACACAG 
AAACTAATTG AGACATTGAA ACCCTTTGGG GTTTTTGAAG AGGAAGAGGA ACTGCAGCGC 
AGGATTTTAA TTTTGGGAAA ACTAAATAAC CTGGTAAAAG AGTGGATACG AGAAATCAGT 
GAAAGCAAGA ATCTTCCACA ATCTGTAATT GAAAATGTTG GAGGAAAAAT TTTTACATTT 
GGATCTTACA GATTAGGAGT GCATACAAAA GGTGCTGATA TTGATGCGTT GTGTGTTGCA 
CCAAGACATG TTGATCGAAG TGACTTTTTC ACCTCATTCT ATGATAAGTT GAAATTACAG 
GAAGAAGTAA AAGATTTAAG AGCTGTTGAA GAGGCATTCG TACCAGTTAT TAAACTCTGT 
TTTGATGGGA TAGAGATTGA TATTTTGTTT GCAAGATTAG CACTGCAGAC AATTCCTGAA 
GATTTGGATC TACGAGATGA CAGTCTGCTA AAAAATTTAG ATATAAGATG TATAAGAAGT 
CTTAACGGTT GCAGGGTAAC CGATGAAATT TTACATCTAG TACCAAACAT TGACAACTTC 
AGGTTAACTC TGAGAGCTAT CAAACTATGG GCCAAACGCC ACAACATCTA TTCCAATATA 
TTAGGTTTCC TCGGTGGTGT TTCCTGGGCT ATGCTAGTAG CAAGAACTTG CCAGCTTTAT 
CCAAATGCAA TAGCATCAAC TCTTGTACAT AAATTTTTCT TGGTATTTTC TAAATGGGAA 
TGGCCAAATC CAGTGCTATT GAAACAGCCT GAAGAATGCA ATCTTAATTT GCCTGTATGG 
GACCCAAGGG TAAACCCCAG TGATAGGTAC CATCTTATGC CTATAATTAC ACCAGCATAC 
CCACAACAGA ACTCCACGTA CAATGTGTCC GTTTCAACAC GGATGGTCAT GGTTGAGGAG 
TTTAAACAAG GTCTTGCTAT CACAGATGAA ATTTTGCTGA GTAAGGCAGA GTGGTCCAAA 
CTTTTTGAAG CTCCAAACTT CTTTCAAAAG TACAAGCATT ATATTGTACT TCTAGCAAGT 
GCACCAACAG AAAAACAACG CCTGGAATGG GTGGGCTTGG TGGAATCAAA AATCCGAATC 
CTGGTTGGAA GCTTGGAGAA GAATGAATTT ATTACACTGG CTCATGTGAA TCCCCAGTCA 
TTTCCAGCAC CCAAAGAAAA TCCCGACAAG GAAGAATTTC GCACGATGTG GGTGATTGGG 
TTAGTGTTTA AAAAAACAGA AAACTCTGAA AACCTCAGTG TTGATCTCAC CTATGATATT 
CAGTCTTTCA CAGATACAGT TTATAGGCAA GCAATAAACA GCAAGATGTT TGAGGTGGAT 
ATGAAAATTG CTGCAATGCA TGTAAAAAGA AAGCAACTCC ATCAACTACT ACCTAATCAT 
GTGCTTCAGA AAAAGAAAAA GCATTCAACA GAAGGTGTCA AATTGACAGC TCTCAATGAC 
AGCAGCCTCG ACTTGTCTAT GGACAGTGAT AACAGCATGT CTGTGCCTTC ACCTACTAGT 
GCTACGAAGA CCAGTCCATT GAACAGTTCT GGCAGCTCTC AGGGAAACAG TCCTGCTCCA 
GCTGTAACAG CAGCATCTGT GACCAACATA CAGGCTACTG AAGTTTCTGT GCCACAAGTA 
AATTCCAGTG AAAGCTCAGG GGGTACATCG AGTGAAAGCA TTCCTCAAAC TGCCACACAA 
CCAGCCATTT CTCCACCACC AAAGCCTACG GTCTCCAGAG TTGTTTCTTC AACACGTCTG 
GTAAACCCAC CACCTAGATC TTCAGGAAAT GCAGCAACTT CAGGAAATGC AGCAACAAAA 
ATACCTACTC CTATAGTAGG AGTCAAGAGG ACATCCTCAC CTCATAAAGA AGAGAGTCCC 
AAGAAAACCA AAACAGAAGA GGATGAAACA AGTGAAGATG CTAACTGTCT TGCTTTGAGT 
GGACATGATA AAACAGAAGC AAAGGAACAA CTTGATACAG AGACAAGTAC AACTCAATCA 
GAAACTATTC AGACAGCGGC TTCTCTGTTG GCCTCTCAGA AAACATCCAG TACAGACCTT 
TCTGATATCC CTGCTCTCCC TGCAAATCCT ATTCCTGTTA TCAAGAATTC AATAAAACTG 
AGATTGAATC GGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PAPOLA ( NM_001363662.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001350591.1 Homo sapiens poly(A) polymerase alpha (PAPOLA), transcript variant 7, mRNA.

Price & Availability↑

CloneID	OHu47485
Clone ID Related Accession (Same CDS sequence)	XM_005267281.1 , NM_001363662.1 , NM_001363662.2
Accession Version	NM_001363662.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2175bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-14
Organism	Homo sapiens(human)
Product	poly(A) polymerase alpha isoform 6
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL163051.5. On May 30, 2018 this sequence version replaced XM_005267281.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.215306.1, SRR1803614.270313.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END##

ATGCCGTTTC CAGTTACAAC ACAGGGATCA CAACAAACAC AACCGCCACA GAAGCACTAT 
GGCATTACTT CTCCTATCAG CTTAGCAGCC CCCAAGGAGA CTGACTGCGT ACTTACACAG 
AAACTAATTG AGACATTGAA ACCCTTTGGG GTTTTTGAAG AGGAAGAGGA ACTGCAGCGC 
AGGATTTTAA TTTTGGGAAA ACTAAATAAC CTGGTAAAAG AGTGGATACG AGAAATCAGT 
GAAAGCAAGA ATCTTCCACA ATCTGTAATT GAAAATGTTG GAGGAAAAAT TTTTACATTT 
GGATCTTACA GATTAGGAGT GCATACAAAA GGTGCTGATA TTGATGCGTT GTGTGTTGCA 
CCAAGACATG TTGATCGAAG TGACTTTTTC ACCTCATTCT ATGATAAGTT GAAATTACAG 
GAAGAAGTAA AAGATTTAAG AGCTGTTGAA GAGGCATTCG TACCAGTTAT TAAACTCTGT 
TTTGATGGGA TAGAGATTGA TATTTTGTTT GCAAGATTAG CACTGCAGAC AATTCCTGAA 
GATTTGGATC TACGAGATGA CAGTCTGCTA AAAAATTTAG ATATAAGATG TATAAGAAGT 
CTTAACGGTT GCAGGGTAAC CGATGAAATT TTACATCTAG TACCAAACAT TGACAACTTC 
AGGTTAACTC TGAGAGCTAT CAAACTATGG GCCAAACGCC ACAACATCTA TTCCAATATA 
TTAGGTTTCC TCGGTGGTGT TTCCTGGGCT ATGCTAGTAG CAAGAACTTG CCAGCTTTAT 
CCAAATGCAA TAGCATCAAC TCTTGTACAT AAATTTTTCT TGGTATTTTC TAAATGGGAA 
TGGCCAAATC CAGTGCTATT GAAACAGCCT GAAGAATGCA ATCTTAATTT GCCTGTATGG 
GACCCAAGGG TAAACCCCAG TGATAGGTAC CATCTTATGC CTATAATTAC ACCAGCATAC 
CCACAACAGA ACTCCACGTA CAATGTGTCC GTTTCAACAC GGATGGTCAT GGTTGAGGAG 
TTTAAACAAG GTCTTGCTAT CACAGATGAA ATTTTGCTGA GTAAGGCAGA GTGGTCCAAA 
CTTTTTGAAG CTCCAAACTT CTTTCAAAAG TACAAGCATT ATATTGTACT TCTAGCAAGT 
GCACCAACAG AAAAACAACG CCTGGAATGG GTGGGCTTGG TGGAATCAAA AATCCGAATC 
CTGGTTGGAA GCTTGGAGAA GAATGAATTT ATTACACTGG CTCATGTGAA TCCCCAGTCA 
TTTCCAGCAC CCAAAGAAAA TCCCGACAAG GAAGAATTTC GCACGATGTG GGTGATTGGG 
TTAGTGTTTA AAAAAACAGA AAACTCTGAA AACCTCAGTG TTGATCTCAC CTATGATATT 
CAGTCTTTCA CAGATACAGT TTATAGGCAA GCAATAAACA GCAAGATGTT TGAGGTGGAT 
ATGAAAATTG CTGCAATGCA TGTAAAAAGA AAGCAACTCC ATCAACTACT ACCTAATCAT 
GTGCTTCAGA AAAAGAAAAA GCATTCAACA GAAGGTGTCA AATTGACAGC TCTCAATGAC 
AGCAGCCTCG ACTTGTCTAT GGACAGTGAT AACAGCATGT CTGTGCCTTC ACCTACTAGT 
GCTACGAAGA CCAGTCCATT GAACAGTTCT GGCAGCTCTC AGGGCAGAAA CAGTCCTGCT 
CCAGCTGTAA CAGCAGCATC TGTGACCAAC ATACAGGCTA CTGAAGTTTC TGTGCCACAA 
GTAAATTCCA GTGAAAGCTC AGGGGGTACA TCGAGTGAAA GCATTCCTCA AACTGCCACA 
CAACCAGCCA TTTCTCCACC ACCAAAGCCT ACGGTCTCCA GAGTTGTTTC TTCAACACGT 
CTGGTAAACC CACCACCTAG ATCTTCAGGA AATGCAGCAA CTTCAGGAAA TGCAGCAACA 
AAAATACCTA CTCCTATAGT AGGAGTCAAG AGGACATCCT CACCTCATAA AGAAGAGAGT 
CCCAAGAAAA CCAAAACAGA AGAGGAACAA CTTGATACAG AGACAAGTAC AACTCAATCA 
GAAACTATTC AGACAGCGGC TTCTCTGTTG GCCTCTCAGA AAACATCCAG TACAGACCTT 
TCTGATATCC CTGCTCTCCC TGCAAATCCT ATTCCTGTTA TCAAGAATTC AATAAAACTG 
AGATTGAATC GGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001350591.1
CDS	218..2392
Translation	MPFPVTTQGSQQTQPPQKHYGITSPISLAAPKETDCVLTQKLIETLKPFGVFEEEEELQRRILILGKLNNLVKEWIREISESKNLPQSVIENVGGKIFTFGSYRLGVHTKGADIDALCVAPRHVDRSDFFTSFYDKLKLQEEVKDLRAVEEAFVPVIKLCFDGIEIDILFARLALQTIPEDLDLRDDSLLKNLDIRCIRSLNGCRVTDEILHLVPNIDNFRLTLRAIKLWAKRHNIYSNILGFLGGVSWAMLVARTCQLYPNAIASTLVHKFFLVFSKWEWPNPVLLKQPEECNLNLPVWDPRVNPSDRYHLMPIITPAYPQQNSTYNVSVSTRMVMVEEFKQGLAITDEILLSKAEWSKLFEAPNFFQKYKHYIVLLASAPTEKQRLEWVGLVESKIRILVGSLEKNEFITLAHVNPQSFPAPKENPDKEEFRTMWVIGLVFKKTENSENLSVDLTYDIQSFTDTVYRQAINSKMFEVDMKIAAMHVKRKQLHQLLPNHVLQKKKKHSTEGVKLTALNDSSLDLSMDSDNSMSVPSPTSATKTSPLNSSGSSQGRNSPAPAVTAASVTNIQATEVSVPQVNSSESSGGTSSESIPQTATQPAISPPPKPTVSRVVSSTRLVNPPPRSSGNAATSGNAATKIPTPIVGVKRTSSPHKEESPKKTKTEEEQLDTETSTTQSETIQTAASLLASQKTSSTDLSDIPALPANPIPVIKNSIKLRLNR

Target ORF information:

RefSeq Version	NM_001363662.1
Organism	Homo sapiens(human)
Definition	Homo sapiens poly(A) polymerase alpha (PAPOLA), transcript variant 7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001363662.1

ORF Insert Sequence:

ATGCCGTTTC CAGTTACAAC ACAGGGATCA CAACAAACAC AACCGCCACA GAAGCACTAT 
GGCATTACTT CTCCTATCAG CTTAGCAGCC CCCAAGGAGA CTGACTGCGT ACTTACACAG 
AAACTAATTG AGACATTGAA ACCCTTTGGG GTTTTTGAAG AGGAAGAGGA ACTGCAGCGC 
AGGATTTTAA TTTTGGGAAA ACTAAATAAC CTGGTAAAAG AGTGGATACG AGAAATCAGT 
GAAAGCAAGA ATCTTCCACA ATCTGTAATT GAAAATGTTG GAGGAAAAAT TTTTACATTT 
GGATCTTACA GATTAGGAGT GCATACAAAA GGTGCTGATA TTGATGCGTT GTGTGTTGCA 
CCAAGACATG TTGATCGAAG TGACTTTTTC ACCTCATTCT ATGATAAGTT GAAATTACAG 
GAAGAAGTAA AAGATTTAAG AGCTGTTGAA GAGGCATTCG TACCAGTTAT TAAACTCTGT 
TTTGATGGGA TAGAGATTGA TATTTTGTTT GCAAGATTAG CACTGCAGAC AATTCCTGAA 
GATTTGGATC TACGAGATGA CAGTCTGCTA AAAAATTTAG ATATAAGATG TATAAGAAGT 
CTTAACGGTT GCAGGGTAAC CGATGAAATT TTACATCTAG TACCAAACAT TGACAACTTC 
AGGTTAACTC TGAGAGCTAT CAAACTATGG GCCAAACGCC ACAACATCTA TTCCAATATA 
TTAGGTTTCC TCGGTGGTGT TTCCTGGGCT ATGCTAGTAG CAAGAACTTG CCAGCTTTAT 
CCAAATGCAA TAGCATCAAC TCTTGTACAT AAATTTTTCT TGGTATTTTC TAAATGGGAA 
TGGCCAAATC CAGTGCTATT GAAACAGCCT GAAGAATGCA ATCTTAATTT GCCTGTATGG 
GACCCAAGGG TAAACCCCAG TGATAGGTAC CATCTTATGC CTATAATTAC ACCAGCATAC 
CCACAACAGA ACTCCACGTA CAATGTGTCC GTTTCAACAC GGATGGTCAT GGTTGAGGAG 
TTTAAACAAG GTCTTGCTAT CACAGATGAA ATTTTGCTGA GTAAGGCAGA GTGGTCCAAA 
CTTTTTGAAG CTCCAAACTT CTTTCAAAAG TACAAGCATT ATATTGTACT TCTAGCAAGT 
GCACCAACAG AAAAACAACG CCTGGAATGG GTGGGCTTGG TGGAATCAAA AATCCGAATC 
CTGGTTGGAA GCTTGGAGAA GAATGAATTT ATTACACTGG CTCATGTGAA TCCCCAGTCA 
TTTCCAGCAC CCAAAGAAAA TCCCGACAAG GAAGAATTTC GCACGATGTG GGTGATTGGG 
TTAGTGTTTA AAAAAACAGA AAACTCTGAA AACCTCAGTG TTGATCTCAC CTATGATATT 
CAGTCTTTCA CAGATACAGT TTATAGGCAA GCAATAAACA GCAAGATGTT TGAGGTGGAT 
ATGAAAATTG CTGCAATGCA TGTAAAAAGA AAGCAACTCC ATCAACTACT ACCTAATCAT 
GTGCTTCAGA AAAAGAAAAA GCATTCAACA GAAGGTGTCA AATTGACAGC TCTCAATGAC 
AGCAGCCTCG ACTTGTCTAT GGACAGTGAT AACAGCATGT CTGTGCCTTC ACCTACTAGT 
GCTACGAAGA CCAGTCCATT GAACAGTTCT GGCAGCTCTC AGGGCAGAAA CAGTCCTGCT 
CCAGCTGTAA CAGCAGCATC TGTGACCAAC ATACAGGCTA CTGAAGTTTC TGTGCCACAA 
GTAAATTCCA GTGAAAGCTC AGGGGGTACA TCGAGTGAAA GCATTCCTCA AACTGCCACA 
CAACCAGCCA TTTCTCCACC ACCAAAGCCT ACGGTCTCCA GAGTTGTTTC TTCAACACGT 
CTGGTAAACC CACCACCTAG ATCTTCAGGA AATGCAGCAA CTTCAGGAAA TGCAGCAACA 
AAAATACCTA CTCCTATAGT AGGAGTCAAG AGGACATCCT CACCTCATAA AGAAGAGAGT 
CCCAAGAAAA CCAAAACAGA AGAGGAACAA CTTGATACAG AGACAAGTAC AACTCAATCA 
GAAACTATTC AGACAGCGGC TTCTCTGTTG GCCTCTCAGA AAACATCCAG TACAGACCTT 
TCTGATATCC CTGCTCTCCC TGCAAATCCT ATTCCTGTTA TCAAGAATTC AATAAAACTG 
AGATTGAATC GGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PAPOLA ( NM_001363662.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001350591.1 Homo sapiens poly(A) polymerase alpha (PAPOLA), transcript variant 7, mRNA.

Price & Availability↑

CloneID	OHu47485
Clone ID Related Accession (Same CDS sequence)	XM_005267281.1 , NM_001363662.1 , NM_001363662.2
Accession Version	NM_001363662.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2175bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-09
Organism	Homo sapiens(human)
Product	poly(A) polymerase alpha isoform 6
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL163051.5. On Jun 2, 2019 this sequence version replaced NM_001363662.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.117161.1, SRR1803613.184411.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END##

ATGCCGTTTC CAGTTACAAC ACAGGGATCA CAACAAACAC AACCGCCACA GAAGCACTAT 
GGCATTACTT CTCCTATCAG CTTAGCAGCC CCCAAGGAGA CTGACTGCGT ACTTACACAG 
AAACTAATTG AGACATTGAA ACCCTTTGGG GTTTTTGAAG AGGAAGAGGA ACTGCAGCGC 
AGGATTTTAA TTTTGGGAAA ACTAAATAAC CTGGTAAAAG AGTGGATACG AGAAATCAGT 
GAAAGCAAGA ATCTTCCACA ATCTGTAATT GAAAATGTTG GAGGAAAAAT TTTTACATTT 
GGATCTTACA GATTAGGAGT GCATACAAAA GGTGCTGATA TTGATGCGTT GTGTGTTGCA 
CCAAGACATG TTGATCGAAG TGACTTTTTC ACCTCATTCT ATGATAAGTT GAAATTACAG 
GAAGAAGTAA AAGATTTAAG AGCTGTTGAA GAGGCATTCG TACCAGTTAT TAAACTCTGT 
TTTGATGGGA TAGAGATTGA TATTTTGTTT GCAAGATTAG CACTGCAGAC AATTCCTGAA 
GATTTGGATC TACGAGATGA CAGTCTGCTA AAAAATTTAG ATATAAGATG TATAAGAAGT 
CTTAACGGTT GCAGGGTAAC CGATGAAATT TTACATCTAG TACCAAACAT TGACAACTTC 
AGGTTAACTC TGAGAGCTAT CAAACTATGG GCCAAACGCC ACAACATCTA TTCCAATATA 
TTAGGTTTCC TCGGTGGTGT TTCCTGGGCT ATGCTAGTAG CAAGAACTTG CCAGCTTTAT 
CCAAATGCAA TAGCATCAAC TCTTGTACAT AAATTTTTCT TGGTATTTTC TAAATGGGAA 
TGGCCAAATC CAGTGCTATT GAAACAGCCT GAAGAATGCA ATCTTAATTT GCCTGTATGG 
GACCCAAGGG TAAACCCCAG TGATAGGTAC CATCTTATGC CTATAATTAC ACCAGCATAC 
CCACAACAGA ACTCCACGTA CAATGTGTCC GTTTCAACAC GGATGGTCAT GGTTGAGGAG 
TTTAAACAAG GTCTTGCTAT CACAGATGAA ATTTTGCTGA GTAAGGCAGA GTGGTCCAAA 
CTTTTTGAAG CTCCAAACTT CTTTCAAAAG TACAAGCATT ATATTGTACT TCTAGCAAGT 
GCACCAACAG AAAAACAACG CCTGGAATGG GTGGGCTTGG TGGAATCAAA AATCCGAATC 
CTGGTTGGAA GCTTGGAGAA GAATGAATTT ATTACACTGG CTCATGTGAA TCCCCAGTCA 
TTTCCAGCAC CCAAAGAAAA TCCCGACAAG GAAGAATTTC GCACGATGTG GGTGATTGGG 
TTAGTGTTTA AAAAAACAGA AAACTCTGAA AACCTCAGTG TTGATCTCAC CTATGATATT 
CAGTCTTTCA CAGATACAGT TTATAGGCAA GCAATAAACA GCAAGATGTT TGAGGTGGAT 
ATGAAAATTG CTGCAATGCA TGTAAAAAGA AAGCAACTCC ATCAACTACT ACCTAATCAT 
GTGCTTCAGA AAAAGAAAAA GCATTCAACA GAAGGTGTCA AATTGACAGC TCTCAATGAC 
AGCAGCCTCG ACTTGTCTAT GGACAGTGAT AACAGCATGT CTGTGCCTTC ACCTACTAGT 
GCTACGAAGA CCAGTCCATT GAACAGTTCT GGCAGCTCTC AGGGCAGAAA CAGTCCTGCT 
CCAGCTGTAA CAGCAGCATC TGTGACCAAC ATACAGGCTA CTGAAGTTTC TGTGCCACAA 
GTAAATTCCA GTGAAAGCTC AGGGGGTACA TCGAGTGAAA GCATTCCTCA AACTGCCACA 
CAACCAGCCA TTTCTCCACC ACCAAAGCCT ACGGTCTCCA GAGTTGTTTC TTCAACACGT 
CTGGTAAACC CACCACCTAG ATCTTCAGGA AATGCAGCAA CTTCAGGAAA TGCAGCAACA 
AAAATACCTA CTCCTATAGT AGGAGTCAAG AGGACATCCT CACCTCATAA AGAAGAGAGT 
CCCAAGAAAA CCAAAACAGA AGAGGAACAA CTTGATACAG AGACAAGTAC AACTCAATCA 
GAAACTATTC AGACAGCGGC TTCTCTGTTG GCCTCTCAGA AAACATCCAG TACAGACCTT 
TCTGATATCC CTGCTCTCCC TGCAAATCCT ATTCCTGTTA TCAAGAATTC AATAAAACTG 
AGATTGAATC GGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001350591.1
CDS	212..2386
Translation	MPFPVTTQGSQQTQPPQKHYGITSPISLAAPKETDCVLTQKLIETLKPFGVFEEEEELQRRILILGKLNNLVKEWIREISESKNLPQSVIENVGGKIFTFGSYRLGVHTKGADIDALCVAPRHVDRSDFFTSFYDKLKLQEEVKDLRAVEEAFVPVIKLCFDGIEIDILFARLALQTIPEDLDLRDDSLLKNLDIRCIRSLNGCRVTDEILHLVPNIDNFRLTLRAIKLWAKRHNIYSNILGFLGGVSWAMLVARTCQLYPNAIASTLVHKFFLVFSKWEWPNPVLLKQPEECNLNLPVWDPRVNPSDRYHLMPIITPAYPQQNSTYNVSVSTRMVMVEEFKQGLAITDEILLSKAEWSKLFEAPNFFQKYKHYIVLLASAPTEKQRLEWVGLVESKIRILVGSLEKNEFITLAHVNPQSFPAPKENPDKEEFRTMWVIGLVFKKTENSENLSVDLTYDIQSFTDTVYRQAINSKMFEVDMKIAAMHVKRKQLHQLLPNHVLQKKKKHSTEGVKLTALNDSSLDLSMDSDNSMSVPSPTSATKTSPLNSSGSSQGRNSPAPAVTAASVTNIQATEVSVPQVNSSESSGGTSSESIPQTATQPAISPPPKPTVSRVVSSTRLVNPPPRSSGNAATSGNAATKIPTPIVGVKRTSSPHKEESPKKTKTEEEQLDTETSTTQSETIQTAASLLASQKTSSTDLSDIPALPANPIPVIKNSIKLRLNR

Target ORF information:

RefSeq Version	NM_001363662.2
Organism	Homo sapiens(human)
Definition	Homo sapiens poly(A) polymerase alpha (PAPOLA), transcript variant 7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001363662.2

ORF Insert Sequence:

ATGCCGTTTC CAGTTACAAC ACAGGGATCA CAACAAACAC AACCGCCACA GAAGCACTAT 
GGCATTACTT CTCCTATCAG CTTAGCAGCC CCCAAGGAGA CTGACTGCGT ACTTACACAG 
AAACTAATTG AGACATTGAA ACCCTTTGGG GTTTTTGAAG AGGAAGAGGA ACTGCAGCGC 
AGGATTTTAA TTTTGGGAAA ACTAAATAAC CTGGTAAAAG AGTGGATACG AGAAATCAGT 
GAAAGCAAGA ATCTTCCACA ATCTGTAATT GAAAATGTTG GAGGAAAAAT TTTTACATTT 
GGATCTTACA GATTAGGAGT GCATACAAAA GGTGCTGATA TTGATGCGTT GTGTGTTGCA 
CCAAGACATG TTGATCGAAG TGACTTTTTC ACCTCATTCT ATGATAAGTT GAAATTACAG 
GAAGAAGTAA AAGATTTAAG AGCTGTTGAA GAGGCATTCG TACCAGTTAT TAAACTCTGT 
TTTGATGGGA TAGAGATTGA TATTTTGTTT GCAAGATTAG CACTGCAGAC AATTCCTGAA 
GATTTGGATC TACGAGATGA CAGTCTGCTA AAAAATTTAG ATATAAGATG TATAAGAAGT 
CTTAACGGTT GCAGGGTAAC CGATGAAATT TTACATCTAG TACCAAACAT TGACAACTTC 
AGGTTAACTC TGAGAGCTAT CAAACTATGG GCCAAACGCC ACAACATCTA TTCCAATATA 
TTAGGTTTCC TCGGTGGTGT TTCCTGGGCT ATGCTAGTAG CAAGAACTTG CCAGCTTTAT 
CCAAATGCAA TAGCATCAAC TCTTGTACAT AAATTTTTCT TGGTATTTTC TAAATGGGAA 
TGGCCAAATC CAGTGCTATT GAAACAGCCT GAAGAATGCA ATCTTAATTT GCCTGTATGG 
GACCCAAGGG TAAACCCCAG TGATAGGTAC CATCTTATGC CTATAATTAC ACCAGCATAC 
CCACAACAGA ACTCCACGTA CAATGTGTCC GTTTCAACAC GGATGGTCAT GGTTGAGGAG 
TTTAAACAAG GTCTTGCTAT CACAGATGAA ATTTTGCTGA GTAAGGCAGA GTGGTCCAAA 
CTTTTTGAAG CTCCAAACTT CTTTCAAAAG TACAAGCATT ATATTGTACT TCTAGCAAGT 
GCACCAACAG AAAAACAACG CCTGGAATGG GTGGGCTTGG TGGAATCAAA AATCCGAATC 
CTGGTTGGAA GCTTGGAGAA GAATGAATTT ATTACACTGG CTCATGTGAA TCCCCAGTCA 
TTTCCAGCAC CCAAAGAAAA TCCCGACAAG GAAGAATTTC GCACGATGTG GGTGATTGGG 
TTAGTGTTTA AAAAAACAGA AAACTCTGAA AACCTCAGTG TTGATCTCAC CTATGATATT 
CAGTCTTTCA CAGATACAGT TTATAGGCAA GCAATAAACA GCAAGATGTT TGAGGTGGAT 
ATGAAAATTG CTGCAATGCA TGTAAAAAGA AAGCAACTCC ATCAACTACT ACCTAATCAT 
GTGCTTCAGA AAAAGAAAAA GCATTCAACA GAAGGTGTCA AATTGACAGC TCTCAATGAC 
AGCAGCCTCG ACTTGTCTAT GGACAGTGAT AACAGCATGT CTGTGCCTTC ACCTACTAGT 
GCTACGAAGA CCAGTCCATT GAACAGTTCT GGCAGCTCTC AGGGCAGAAA CAGTCCTGCT 
CCAGCTGTAA CAGCAGCATC TGTGACCAAC ATACAGGCTA CTGAAGTTTC TGTGCCACAA 
GTAAATTCCA GTGAAAGCTC AGGGGGTACA TCGAGTGAAA GCATTCCTCA AACTGCCACA 
CAACCAGCCA TTTCTCCACC ACCAAAGCCT ACGGTCTCCA GAGTTGTTTC TTCAACACGT 
CTGGTAAACC CACCACCTAG ATCTTCAGGA AATGCAGCAA CTTCAGGAAA TGCAGCAACA 
AAAATACCTA CTCCTATAGT AGGAGTCAAG AGGACATCCT CACCTCATAA AGAAGAGAGT 
CCCAAGAAAA CCAAAACAGA AGAGGAACAA CTTGATACAG AGACAAGTAC AACTCAATCA 
GAAACTATTC AGACAGCGGC TTCTCTGTTG GCCTCTCAGA AAACATCCAG TACAGACCTT 
TCTGATATCC CTGCTCTCCC TGCAAATCCT ATTCCTGTTA TCAAGAATTC AATAAAACTG 
AGATTGAATC GGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PAPOLA ( NM_001363665.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001350594.1 Homo sapiens poly(A) polymerase alpha (PAPOLA), transcript variant 9, mRNA.

Price & Availability↑

CloneID	OHu47486
Clone ID Related Accession (Same CDS sequence)	NM_001363665.1 , NM_001363665.2 , XM_005267282.2
Accession Version	NM_001363665.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2100bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-07-01
Organism	Homo sapiens(human)
Product	poly(A) polymerase alpha isoform 8
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL163051.5. On May 29, 2018 this sequence version replaced XM_005267282.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.26213.1, SRR1660809.158700.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END##

ATGCCGTTTC CAGTTACAAC ACAGGGATCA CAACAAACAC AACCGCCACA GAAGCACTAT 
GGCATTACTT CTCCTATCAG CTTAGCAGCC CCCAAGGAGA CTGACTGCGT ACTTACACAG 
AAACTAATTG AGACATTGAA ACCCTTTGGG GTTTTTGAAG AGGAAGAGGA ACTGCAGCGC 
AGGATTTTAA TTTTGGGAAA ACTAAATAAC CTGGTAAAAG AGTGGATACG AGAAATCAGT 
GAAAGCAAGA ATCTTCCACA ATCTGTAATT GAAAATGTTG GAGGAAAAAT TTTTACATTT 
GGATCTTACA GATTAGGAGT GCATACAAAA GGTGCTGATA TTGATGCGTT GTGTGTTGCA 
CCAAGACATG TTGATCGAAG TGACTTTTTC ACCTCATTCT ATGATAAGTT GAAATTACAG 
GAAGAAGTAA AAGATTTAAG AGCTGTTGAA GAGGCATTCG TACCAGTTAT TAAACTCTGT 
TTTGATGGGA TAGAGATTGA TATTTTGTTT GCAAGATTAG CACTGCAGAC AATTCCTGAA 
GATTTGGATC TACGAGATGA CAGTCTGCTA AAAAATTTAG ATATAAGATG TATAAGAAGT 
CTTAACGGTT GCAGGGTAAC CGATGAAATT TTACATCTAG TACCAAACAT TGACAACTTC 
AGGTTAACTC TGAGAGCTAT CAAACTATGG GCCAAACGCC ACAACATCTA TTCCAATATA 
TTAGGTTTCC TCGGTGGTGT TTCCTGGGCT ATGCTAGTAG CAAGAACTTG CCAGCTTTAT 
CCAAATGCAA TAGCATCAAC TCTTGTACAT AAATTTTTCT TGGTATTTTC TAAATGGGAA 
TGGCCAAATC CAGTGCTATT GAAACAGCCT GAAGAATGCA ATCTTAATTT GCCTGTATGG 
GACCCAAGGG TAAACCCCAG TGATAGGTAC CATCTTATGC CTATAATTAC ACCAGCATAC 
CCACAACAGA ACTCCACGTA CAATGTGTCC GTTTCAACAC GGATGGTCAT GGTTGAGGAG 
TTTAAACAAG GTCTTGCTAT CACAGATGAA ATTTTGCTGA GTAAGGCAGA GTGGTCCAAA 
CTTTTTGAAG CTCCAAACTT CTTTCAAAAG TACAAGCATT ATATTGTACT TCTAGCAAGT 
GCACCAACAG AAAAACAACG CCTGGAATGG GTGGGCTTGG TGGAATCAAA AATCCGAATC 
CTGGTTGGAA GCTTGGAGAA GAATGAATTT ATTACACTGG CTCATGTGAA TCCCCAGTCA 
TTTCCAGCAC CCAAAGAAAA TCCCGACAAG GAAGAATTTC GCACGATGTG GGTGATTGGG 
TTAGTGTTTA AAAAAACAGA AAACTCTGAA AACCTCAGTG TTGATCTCAC CTATGATATT 
CAGTCTTTCA CAGATACAGT TTATAGGCAA GCAATAAACA GCAAGATGTT TGAGGTGGAT 
ATGAAAATTG CTGCAATGCA TGTAAAAAGA AAGCAACTCC ATCAACTACT ACCTAATCAT 
GTGCTTCAGA AAAAGAAAAA GCATTCAACA GAAGGTGTCA AATTGACAGC TCTCAATGAC 
AGCAGCCTCG ACTTGTCTAT GGACAGTGAT AACAGCATGT CTGTGCCTTC ACCTACTAGT 
GCTACGAAGA CCAGTCCATT GAACAGTTCT GGCAGCTCTC AGGGCAGAAA CAGTCCTGCT 
CCAGCTGTAA CAGCAGCATC TGTGACCAAC ATACAGGCTA CTGAAGTTTC TGTGCCACAA 
GTAAATTCCA GTGAAAGCTC AGGGGGTACA TCGAGTGAAA GCATTCCTCA AACTGCCACA 
CAACCAGCCA TTTCTCCACC ACCAAAGCCT ACGGTCTCCA GAGTTGTTTC TTCAACACGT 
CTGGTAAACC CACCACCTAG ATCTTCAGGA AATGCAGCAA CTTCAGGAAA TGCAGCAACA 
AAAATACCTA CTCCTATAGT AGGAGTCAAG AGGACATCCT CACCTCATAA AGAAGAGAGT 
CCCAAGAAAA CCAAAACAGA AGAGAAAACA TCCAGTACAG ACCTTTCTGA TATCCCTGCT 
CTCCCTGCAA ATCCTATTCC TGTTATCAAG AATTCAATAA AACTGAGATT GAATCGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001350594.1
CDS	218..2317
Translation	MPFPVTTQGSQQTQPPQKHYGITSPISLAAPKETDCVLTQKLIETLKPFGVFEEEEELQRRILILGKLNNLVKEWIREISESKNLPQSVIENVGGKIFTFGSYRLGVHTKGADIDALCVAPRHVDRSDFFTSFYDKLKLQEEVKDLRAVEEAFVPVIKLCFDGIEIDILFARLALQTIPEDLDLRDDSLLKNLDIRCIRSLNGCRVTDEILHLVPNIDNFRLTLRAIKLWAKRHNIYSNILGFLGGVSWAMLVARTCQLYPNAIASTLVHKFFLVFSKWEWPNPVLLKQPEECNLNLPVWDPRVNPSDRYHLMPIITPAYPQQNSTYNVSVSTRMVMVEEFKQGLAITDEILLSKAEWSKLFEAPNFFQKYKHYIVLLASAPTEKQRLEWVGLVESKIRILVGSLEKNEFITLAHVNPQSFPAPKENPDKEEFRTMWVIGLVFKKTENSENLSVDLTYDIQSFTDTVYRQAINSKMFEVDMKIAAMHVKRKQLHQLLPNHVLQKKKKHSTEGVKLTALNDSSLDLSMDSDNSMSVPSPTSATKTSPLNSSGSSQGRNSPAPAVTAASVTNIQATEVSVPQVNSSESSGGTSSESIPQTATQPAISPPPKPTVSRVVSSTRLVNPPPRSSGNAATSGNAATKIPTPIVGVKRTSSPHKEESPKKTKTEEKTSSTDLSDIPALPANPIPVIKNSIKLRLNR

Target ORF information:

RefSeq Version	NM_001363665.1
Organism	Homo sapiens(human)
Definition	Homo sapiens poly(A) polymerase alpha (PAPOLA), transcript variant 9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001363665.1

ORF Insert Sequence:

ATGCCGTTTC CAGTTACAAC ACAGGGATCA CAACAAACAC AACCGCCACA GAAGCACTAT 
GGCATTACTT CTCCTATCAG CTTAGCAGCC CCCAAGGAGA CTGACTGCGT ACTTACACAG 
AAACTAATTG AGACATTGAA ACCCTTTGGG GTTTTTGAAG AGGAAGAGGA ACTGCAGCGC 
AGGATTTTAA TTTTGGGAAA ACTAAATAAC CTGGTAAAAG AGTGGATACG AGAAATCAGT 
GAAAGCAAGA ATCTTCCACA ATCTGTAATT GAAAATGTTG GAGGAAAAAT TTTTACATTT 
GGATCTTACA GATTAGGAGT GCATACAAAA GGTGCTGATA TTGATGCGTT GTGTGTTGCA 
CCAAGACATG TTGATCGAAG TGACTTTTTC ACCTCATTCT ATGATAAGTT GAAATTACAG 
GAAGAAGTAA AAGATTTAAG AGCTGTTGAA GAGGCATTCG TACCAGTTAT TAAACTCTGT 
TTTGATGGGA TAGAGATTGA TATTTTGTTT GCAAGATTAG CACTGCAGAC AATTCCTGAA 
GATTTGGATC TACGAGATGA CAGTCTGCTA AAAAATTTAG ATATAAGATG TATAAGAAGT 
CTTAACGGTT GCAGGGTAAC CGATGAAATT TTACATCTAG TACCAAACAT TGACAACTTC 
AGGTTAACTC TGAGAGCTAT CAAACTATGG GCCAAACGCC ACAACATCTA TTCCAATATA 
TTAGGTTTCC TCGGTGGTGT TTCCTGGGCT ATGCTAGTAG CAAGAACTTG CCAGCTTTAT 
CCAAATGCAA TAGCATCAAC TCTTGTACAT AAATTTTTCT TGGTATTTTC TAAATGGGAA 
TGGCCAAATC CAGTGCTATT GAAACAGCCT GAAGAATGCA ATCTTAATTT GCCTGTATGG 
GACCCAAGGG TAAACCCCAG TGATAGGTAC CATCTTATGC CTATAATTAC ACCAGCATAC 
CCACAACAGA ACTCCACGTA CAATGTGTCC GTTTCAACAC GGATGGTCAT GGTTGAGGAG 
TTTAAACAAG GTCTTGCTAT CACAGATGAA ATTTTGCTGA GTAAGGCAGA GTGGTCCAAA 
CTTTTTGAAG CTCCAAACTT CTTTCAAAAG TACAAGCATT ATATTGTACT TCTAGCAAGT 
GCACCAACAG AAAAACAACG CCTGGAATGG GTGGGCTTGG TGGAATCAAA AATCCGAATC 
CTGGTTGGAA GCTTGGAGAA GAATGAATTT ATTACACTGG CTCATGTGAA TCCCCAGTCA 
TTTCCAGCAC CCAAAGAAAA TCCCGACAAG GAAGAATTTC GCACGATGTG GGTGATTGGG 
TTAGTGTTTA AAAAAACAGA AAACTCTGAA AACCTCAGTG TTGATCTCAC CTATGATATT 
CAGTCTTTCA CAGATACAGT TTATAGGCAA GCAATAAACA GCAAGATGTT TGAGGTGGAT 
ATGAAAATTG CTGCAATGCA TGTAAAAAGA AAGCAACTCC ATCAACTACT ACCTAATCAT 
GTGCTTCAGA AAAAGAAAAA GCATTCAACA GAAGGTGTCA AATTGACAGC TCTCAATGAC 
AGCAGCCTCG ACTTGTCTAT GGACAGTGAT AACAGCATGT CTGTGCCTTC ACCTACTAGT 
GCTACGAAGA CCAGTCCATT GAACAGTTCT GGCAGCTCTC AGGGCAGAAA CAGTCCTGCT 
CCAGCTGTAA CAGCAGCATC TGTGACCAAC ATACAGGCTA CTGAAGTTTC TGTGCCACAA 
GTAAATTCCA GTGAAAGCTC AGGGGGTACA TCGAGTGAAA GCATTCCTCA AACTGCCACA 
CAACCAGCCA TTTCTCCACC ACCAAAGCCT ACGGTCTCCA GAGTTGTTTC TTCAACACGT 
CTGGTAAACC CACCACCTAG ATCTTCAGGA AATGCAGCAA CTTCAGGAAA TGCAGCAACA 
AAAATACCTA CTCCTATAGT AGGAGTCAAG AGGACATCCT CACCTCATAA AGAAGAGAGT 
CCCAAGAAAA CCAAAACAGA AGAGAAAACA TCCAGTACAG ACCTTTCTGA TATCCCTGCT 
CTCCCTGCAA ATCCTATTCC TGTTATCAAG AATTCAATAA AACTGAGATT GAATCGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PAPOLA ( NM_001363665.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001350594.1 Homo sapiens poly(A) polymerase alpha (PAPOLA), transcript variant 9, mRNA.

Price & Availability↑

CloneID	OHu47486
Clone ID Related Accession (Same CDS sequence)	NM_001363665.1 , NM_001363665.2 , XM_005267282.2
Accession Version	NM_001363665.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2100bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-09
Organism	Homo sapiens(human)
Product	poly(A) polymerase alpha isoform 8
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL163051.5. On Jun 2, 2019 this sequence version replaced NM_001363665.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.160662.1, SRR1803613.293.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END##

ATGCCGTTTC CAGTTACAAC ACAGGGATCA CAACAAACAC AACCGCCACA GAAGCACTAT 
GGCATTACTT CTCCTATCAG CTTAGCAGCC CCCAAGGAGA CTGACTGCGT ACTTACACAG 
AAACTAATTG AGACATTGAA ACCCTTTGGG GTTTTTGAAG AGGAAGAGGA ACTGCAGCGC 
AGGATTTTAA TTTTGGGAAA ACTAAATAAC CTGGTAAAAG AGTGGATACG AGAAATCAGT 
GAAAGCAAGA ATCTTCCACA ATCTGTAATT GAAAATGTTG GAGGAAAAAT TTTTACATTT 
GGATCTTACA GATTAGGAGT GCATACAAAA GGTGCTGATA TTGATGCGTT GTGTGTTGCA 
CCAAGACATG TTGATCGAAG TGACTTTTTC ACCTCATTCT ATGATAAGTT GAAATTACAG 
GAAGAAGTAA AAGATTTAAG AGCTGTTGAA GAGGCATTCG TACCAGTTAT TAAACTCTGT 
TTTGATGGGA TAGAGATTGA TATTTTGTTT GCAAGATTAG CACTGCAGAC AATTCCTGAA 
GATTTGGATC TACGAGATGA CAGTCTGCTA AAAAATTTAG ATATAAGATG TATAAGAAGT 
CTTAACGGTT GCAGGGTAAC CGATGAAATT TTACATCTAG TACCAAACAT TGACAACTTC 
AGGTTAACTC TGAGAGCTAT CAAACTATGG GCCAAACGCC ACAACATCTA TTCCAATATA 
TTAGGTTTCC TCGGTGGTGT TTCCTGGGCT ATGCTAGTAG CAAGAACTTG CCAGCTTTAT 
CCAAATGCAA TAGCATCAAC TCTTGTACAT AAATTTTTCT TGGTATTTTC TAAATGGGAA 
TGGCCAAATC CAGTGCTATT GAAACAGCCT GAAGAATGCA ATCTTAATTT GCCTGTATGG 
GACCCAAGGG TAAACCCCAG TGATAGGTAC CATCTTATGC CTATAATTAC ACCAGCATAC 
CCACAACAGA ACTCCACGTA CAATGTGTCC GTTTCAACAC GGATGGTCAT GGTTGAGGAG 
TTTAAACAAG GTCTTGCTAT CACAGATGAA ATTTTGCTGA GTAAGGCAGA GTGGTCCAAA 
CTTTTTGAAG CTCCAAACTT CTTTCAAAAG TACAAGCATT ATATTGTACT TCTAGCAAGT 
GCACCAACAG AAAAACAACG CCTGGAATGG GTGGGCTTGG TGGAATCAAA AATCCGAATC 
CTGGTTGGAA GCTTGGAGAA GAATGAATTT ATTACACTGG CTCATGTGAA TCCCCAGTCA 
TTTCCAGCAC CCAAAGAAAA TCCCGACAAG GAAGAATTTC GCACGATGTG GGTGATTGGG 
TTAGTGTTTA AAAAAACAGA AAACTCTGAA AACCTCAGTG TTGATCTCAC CTATGATATT 
CAGTCTTTCA CAGATACAGT TTATAGGCAA GCAATAAACA GCAAGATGTT TGAGGTGGAT 
ATGAAAATTG CTGCAATGCA TGTAAAAAGA AAGCAACTCC ATCAACTACT ACCTAATCAT 
GTGCTTCAGA AAAAGAAAAA GCATTCAACA GAAGGTGTCA AATTGACAGC TCTCAATGAC 
AGCAGCCTCG ACTTGTCTAT GGACAGTGAT AACAGCATGT CTGTGCCTTC ACCTACTAGT 
GCTACGAAGA CCAGTCCATT GAACAGTTCT GGCAGCTCTC AGGGCAGAAA CAGTCCTGCT 
CCAGCTGTAA CAGCAGCATC TGTGACCAAC ATACAGGCTA CTGAAGTTTC TGTGCCACAA 
GTAAATTCCA GTGAAAGCTC AGGGGGTACA TCGAGTGAAA GCATTCCTCA AACTGCCACA 
CAACCAGCCA TTTCTCCACC ACCAAAGCCT ACGGTCTCCA GAGTTGTTTC TTCAACACGT 
CTGGTAAACC CACCACCTAG ATCTTCAGGA AATGCAGCAA CTTCAGGAAA TGCAGCAACA 
AAAATACCTA CTCCTATAGT AGGAGTCAAG AGGACATCCT CACCTCATAA AGAAGAGAGT 
CCCAAGAAAA CCAAAACAGA AGAGAAAACA TCCAGTACAG ACCTTTCTGA TATCCCTGCT 
CTCCCTGCAA ATCCTATTCC TGTTATCAAG AATTCAATAA AACTGAGATT GAATCGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001350594.1
CDS	212..2311
Translation	MPFPVTTQGSQQTQPPQKHYGITSPISLAAPKETDCVLTQKLIETLKPFGVFEEEEELQRRILILGKLNNLVKEWIREISESKNLPQSVIENVGGKIFTFGSYRLGVHTKGADIDALCVAPRHVDRSDFFTSFYDKLKLQEEVKDLRAVEEAFVPVIKLCFDGIEIDILFARLALQTIPEDLDLRDDSLLKNLDIRCIRSLNGCRVTDEILHLVPNIDNFRLTLRAIKLWAKRHNIYSNILGFLGGVSWAMLVARTCQLYPNAIASTLVHKFFLVFSKWEWPNPVLLKQPEECNLNLPVWDPRVNPSDRYHLMPIITPAYPQQNSTYNVSVSTRMVMVEEFKQGLAITDEILLSKAEWSKLFEAPNFFQKYKHYIVLLASAPTEKQRLEWVGLVESKIRILVGSLEKNEFITLAHVNPQSFPAPKENPDKEEFRTMWVIGLVFKKTENSENLSVDLTYDIQSFTDTVYRQAINSKMFEVDMKIAAMHVKRKQLHQLLPNHVLQKKKKHSTEGVKLTALNDSSLDLSMDSDNSMSVPSPTSATKTSPLNSSGSSQGRNSPAPAVTAASVTNIQATEVSVPQVNSSESSGGTSSESIPQTATQPAISPPPKPTVSRVVSSTRLVNPPPRSSGNAATSGNAATKIPTPIVGVKRTSSPHKEESPKKTKTEEKTSSTDLSDIPALPANPIPVIKNSIKLRLNR

Target ORF information:

RefSeq Version	NM_001363665.2
Organism	Homo sapiens(human)
Definition	Homo sapiens poly(A) polymerase alpha (PAPOLA), transcript variant 9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001363665.2

ORF Insert Sequence:

ATGCCGTTTC CAGTTACAAC ACAGGGATCA CAACAAACAC AACCGCCACA GAAGCACTAT 
GGCATTACTT CTCCTATCAG CTTAGCAGCC CCCAAGGAGA CTGACTGCGT ACTTACACAG 
AAACTAATTG AGACATTGAA ACCCTTTGGG GTTTTTGAAG AGGAAGAGGA ACTGCAGCGC 
AGGATTTTAA TTTTGGGAAA ACTAAATAAC CTGGTAAAAG AGTGGATACG AGAAATCAGT 
GAAAGCAAGA ATCTTCCACA ATCTGTAATT GAAAATGTTG GAGGAAAAAT TTTTACATTT 
GGATCTTACA GATTAGGAGT GCATACAAAA GGTGCTGATA TTGATGCGTT GTGTGTTGCA 
CCAAGACATG TTGATCGAAG TGACTTTTTC ACCTCATTCT ATGATAAGTT GAAATTACAG 
GAAGAAGTAA AAGATTTAAG AGCTGTTGAA GAGGCATTCG TACCAGTTAT TAAACTCTGT 
TTTGATGGGA TAGAGATTGA TATTTTGTTT GCAAGATTAG CACTGCAGAC AATTCCTGAA 
GATTTGGATC TACGAGATGA CAGTCTGCTA AAAAATTTAG ATATAAGATG TATAAGAAGT 
CTTAACGGTT GCAGGGTAAC CGATGAAATT TTACATCTAG TACCAAACAT TGACAACTTC 
AGGTTAACTC TGAGAGCTAT CAAACTATGG GCCAAACGCC ACAACATCTA TTCCAATATA 
TTAGGTTTCC TCGGTGGTGT TTCCTGGGCT ATGCTAGTAG CAAGAACTTG CCAGCTTTAT 
CCAAATGCAA TAGCATCAAC TCTTGTACAT AAATTTTTCT TGGTATTTTC TAAATGGGAA 
TGGCCAAATC CAGTGCTATT GAAACAGCCT GAAGAATGCA ATCTTAATTT GCCTGTATGG 
GACCCAAGGG TAAACCCCAG TGATAGGTAC CATCTTATGC CTATAATTAC ACCAGCATAC 
CCACAACAGA ACTCCACGTA CAATGTGTCC GTTTCAACAC GGATGGTCAT GGTTGAGGAG 
TTTAAACAAG GTCTTGCTAT CACAGATGAA ATTTTGCTGA GTAAGGCAGA GTGGTCCAAA 
CTTTTTGAAG CTCCAAACTT CTTTCAAAAG TACAAGCATT ATATTGTACT TCTAGCAAGT 
GCACCAACAG AAAAACAACG CCTGGAATGG GTGGGCTTGG TGGAATCAAA AATCCGAATC 
CTGGTTGGAA GCTTGGAGAA GAATGAATTT ATTACACTGG CTCATGTGAA TCCCCAGTCA 
TTTCCAGCAC CCAAAGAAAA TCCCGACAAG GAAGAATTTC GCACGATGTG GGTGATTGGG 
TTAGTGTTTA AAAAAACAGA AAACTCTGAA AACCTCAGTG TTGATCTCAC CTATGATATT 
CAGTCTTTCA CAGATACAGT TTATAGGCAA GCAATAAACA GCAAGATGTT TGAGGTGGAT 
ATGAAAATTG CTGCAATGCA TGTAAAAAGA AAGCAACTCC ATCAACTACT ACCTAATCAT 
GTGCTTCAGA AAAAGAAAAA GCATTCAACA GAAGGTGTCA AATTGACAGC TCTCAATGAC 
AGCAGCCTCG ACTTGTCTAT GGACAGTGAT AACAGCATGT CTGTGCCTTC ACCTACTAGT 
GCTACGAAGA CCAGTCCATT GAACAGTTCT GGCAGCTCTC AGGGCAGAAA CAGTCCTGCT 
CCAGCTGTAA CAGCAGCATC TGTGACCAAC ATACAGGCTA CTGAAGTTTC TGTGCCACAA 
GTAAATTCCA GTGAAAGCTC AGGGGGTACA TCGAGTGAAA GCATTCCTCA AACTGCCACA 
CAACCAGCCA TTTCTCCACC ACCAAAGCCT ACGGTCTCCA GAGTTGTTTC TTCAACACGT 
CTGGTAAACC CACCACCTAG ATCTTCAGGA AATGCAGCAA CTTCAGGAAA TGCAGCAACA 
AAAATACCTA CTCCTATAGT AGGAGTCAAG AGGACATCCT CACCTCATAA AGAAGAGAGT 
CCCAAGAAAA CCAAAACAGA AGAGAAAACA TCCAGTACAG ACCTTTCTGA TATCCCTGCT 
CTCCCTGCAA ATCCTATTCC TGTTATCAAG AATTCAATAA AACTGAGATT GAATCGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PAPOLA ( XM_005267281.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_005267338.1 Homo sapiens poly(A) polymerase alpha (PAPOLA), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu47485
Clone ID Related Accession (Same CDS sequence)	XM_005267281.1 , NM_001363662.1 , NM_001363662.2
Accession Version	XM_005267281.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2175bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	poly(A) polymerase alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGTTTC CAGTTACAAC ACAGGGATCA CAACAAACAC AACCGCCACA GAAGCACTAT 
GGCATTACTT CTCCTATCAG CTTAGCAGCC CCCAAGGAGA CTGACTGCGT ACTTACACAG 
AAACTAATTG AGACATTGAA ACCCTTTGGG GTTTTTGAAG AGGAAGAGGA ACTGCAGCGC 
AGGATTTTAA TTTTGGGAAA ACTAAATAAC CTGGTAAAAG AGTGGATACG AGAAATCAGT 
GAAAGCAAGA ATCTTCCACA ATCTGTAATT GAAAATGTTG GAGGAAAAAT TTTTACATTT 
GGATCTTACA GATTAGGAGT GCATACAAAA GGTGCTGATA TTGATGCGTT GTGTGTTGCA 
CCAAGACATG TTGATCGAAG TGACTTTTTC ACCTCATTCT ATGATAAGTT GAAATTACAG 
GAAGAAGTAA AAGATTTAAG AGCTGTTGAA GAGGCATTCG TACCAGTTAT TAAACTCTGT 
TTTGATGGGA TAGAGATTGA TATTTTGTTT GCAAGATTAG CACTGCAGAC AATTCCTGAA 
GATTTGGATC TACGAGATGA CAGTCTGCTA AAAAATTTAG ATATAAGATG TATAAGAAGT 
CTTAACGGTT GCAGGGTAAC CGATGAAATT TTACATCTAG TACCAAACAT TGACAACTTC 
AGGTTAACTC TGAGAGCTAT CAAACTATGG GCCAAACGCC ACAACATCTA TTCCAATATA 
TTAGGTTTCC TCGGTGGTGT TTCCTGGGCT ATGCTAGTAG CAAGAACTTG CCAGCTTTAT 
CCAAATGCAA TAGCATCAAC TCTTGTACAT AAATTTTTCT TGGTATTTTC TAAATGGGAA 
TGGCCAAATC CAGTGCTATT GAAACAGCCT GAAGAATGCA ATCTTAATTT GCCTGTATGG 
GACCCAAGGG TAAACCCCAG TGATAGGTAC CATCTTATGC CTATAATTAC ACCAGCATAC 
CCACAACAGA ACTCCACGTA CAATGTGTCC GTTTCAACAC GGATGGTCAT GGTTGAGGAG 
TTTAAACAAG GTCTTGCTAT CACAGATGAA ATTTTGCTGA GTAAGGCAGA GTGGTCCAAA 
CTTTTTGAAG CTCCAAACTT CTTTCAAAAG TACAAGCATT ATATTGTACT TCTAGCAAGT 
GCACCAACAG AAAAACAACG CCTGGAATGG GTGGGCTTGG TGGAATCAAA AATCCGAATC 
CTGGTTGGAA GCTTGGAGAA GAATGAATTT ATTACACTGG CTCATGTGAA TCCCCAGTCA 
TTTCCAGCAC CCAAAGAAAA TCCCGACAAG GAAGAATTTC GCACGATGTG GGTGATTGGG 
TTAGTGTTTA AAAAAACAGA AAACTCTGAA AACCTCAGTG TTGATCTCAC CTATGATATT 
CAGTCTTTCA CAGATACAGT TTATAGGCAA GCAATAAACA GCAAGATGTT TGAGGTGGAT 
ATGAAAATTG CTGCAATGCA TGTAAAAAGA AAGCAACTCC ATCAACTACT ACCTAATCAT 
GTGCTTCAGA AAAAGAAAAA GCATTCAACA GAAGGTGTCA AATTGACAGC TCTCAATGAC 
AGCAGCCTCG ACTTGTCTAT GGACAGTGAT AACAGCATGT CTGTGCCTTC ACCTACTAGT 
GCTACGAAGA CCAGTCCATT GAACAGTTCT GGCAGCTCTC AGGGCAGAAA CAGTCCTGCT 
CCAGCTGTAA CAGCAGCATC TGTGACCAAC ATACAGGCTA CTGAAGTTTC TGTGCCACAA 
GTAAATTCCA GTGAAAGCTC AGGGGGTACA TCGAGTGAAA GCATTCCTCA AACTGCCACA 
CAACCAGCCA TTTCTCCACC ACCAAAGCCT ACGGTCTCCA GAGTTGTTTC TTCAACACGT 
CTGGTAAACC CACCACCTAG ATCTTCAGGA AATGCAGCAA CTTCAGGAAA TGCAGCAACA 
AAAATACCTA CTCCTATAGT AGGAGTCAAG AGGACATCCT CACCTCATAA AGAAGAGAGT 
CCCAAGAAAA CCAAAACAGA AGAGGAACAA CTTGATACAG AGACAAGTAC AACTCAATCA 
GAAACTATTC AGACAGCGGC TTCTCTGTTG GCCTCTCAGA AAACATCCAG TACAGACCTT 
TCTGATATCC CTGCTCTCCC TGCAAATCCT ATTCCTGTTA TCAAGAATTC AATAAAACTG 
AGATTGAATC GGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005267338.1
CDS	214..2388
Translation	MPFPVTTQGSQQTQPPQKHYGITSPISLAAPKETDCVLTQKLIETLKPFGVFEEEEELQRRILILGKLNNLVKEWIREISESKNLPQSVIENVGGKIFTFGSYRLGVHTKGADIDALCVAPRHVDRSDFFTSFYDKLKLQEEVKDLRAVEEAFVPVIKLCFDGIEIDILFARLALQTIPEDLDLRDDSLLKNLDIRCIRSLNGCRVTDEILHLVPNIDNFRLTLRAIKLWAKRHNIYSNILGFLGGVSWAMLVARTCQLYPNAIASTLVHKFFLVFSKWEWPNPVLLKQPEECNLNLPVWDPRVNPSDRYHLMPIITPAYPQQNSTYNVSVSTRMVMVEEFKQGLAITDEILLSKAEWSKLFEAPNFFQKYKHYIVLLASAPTEKQRLEWVGLVESKIRILVGSLEKNEFITLAHVNPQSFPAPKENPDKEEFRTMWVIGLVFKKTENSENLSVDLTYDIQSFTDTVYRQAINSKMFEVDMKIAAMHVKRKQLHQLLPNHVLQKKKKHSTEGVKLTALNDSSLDLSMDSDNSMSVPSPTSATKTSPLNSSGSSQGRNSPAPAVTAASVTNIQATEVSVPQVNSSESSGGTSSESIPQTATQPAISPPPKPTVSRVVSSTRLVNPPPRSSGNAATSGNAATKIPTPIVGVKRTSSPHKEESPKKTKTEEEQLDTETSTTQSETIQTAASLLASQKTSSTDLSDIPALPANPIPVIKNSIKLRLNR

Target ORF information:

RefSeq Version	XM_005267281.1
Organism	Homo sapiens(human)
Definition	Homo sapiens poly(A) polymerase alpha (PAPOLA), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005267281.1

ORF Insert Sequence:

ATGCCGTTTC CAGTTACAAC ACAGGGATCA CAACAAACAC AACCGCCACA GAAGCACTAT 
GGCATTACTT CTCCTATCAG CTTAGCAGCC CCCAAGGAGA CTGACTGCGT ACTTACACAG 
AAACTAATTG AGACATTGAA ACCCTTTGGG GTTTTTGAAG AGGAAGAGGA ACTGCAGCGC 
AGGATTTTAA TTTTGGGAAA ACTAAATAAC CTGGTAAAAG AGTGGATACG AGAAATCAGT 
GAAAGCAAGA ATCTTCCACA ATCTGTAATT GAAAATGTTG GAGGAAAAAT TTTTACATTT 
GGATCTTACA GATTAGGAGT GCATACAAAA GGTGCTGATA TTGATGCGTT GTGTGTTGCA 
CCAAGACATG TTGATCGAAG TGACTTTTTC ACCTCATTCT ATGATAAGTT GAAATTACAG 
GAAGAAGTAA AAGATTTAAG AGCTGTTGAA GAGGCATTCG TACCAGTTAT TAAACTCTGT 
TTTGATGGGA TAGAGATTGA TATTTTGTTT GCAAGATTAG CACTGCAGAC AATTCCTGAA 
GATTTGGATC TACGAGATGA CAGTCTGCTA AAAAATTTAG ATATAAGATG TATAAGAAGT 
CTTAACGGTT GCAGGGTAAC CGATGAAATT TTACATCTAG TACCAAACAT TGACAACTTC 
AGGTTAACTC TGAGAGCTAT CAAACTATGG GCCAAACGCC ACAACATCTA TTCCAATATA 
TTAGGTTTCC TCGGTGGTGT TTCCTGGGCT ATGCTAGTAG CAAGAACTTG CCAGCTTTAT 
CCAAATGCAA TAGCATCAAC TCTTGTACAT AAATTTTTCT TGGTATTTTC TAAATGGGAA 
TGGCCAAATC CAGTGCTATT GAAACAGCCT GAAGAATGCA ATCTTAATTT GCCTGTATGG 
GACCCAAGGG TAAACCCCAG TGATAGGTAC CATCTTATGC CTATAATTAC ACCAGCATAC 
CCACAACAGA ACTCCACGTA CAATGTGTCC GTTTCAACAC GGATGGTCAT GGTTGAGGAG 
TTTAAACAAG GTCTTGCTAT CACAGATGAA ATTTTGCTGA GTAAGGCAGA GTGGTCCAAA 
CTTTTTGAAG CTCCAAACTT CTTTCAAAAG TACAAGCATT ATATTGTACT TCTAGCAAGT 
GCACCAACAG AAAAACAACG CCTGGAATGG GTGGGCTTGG TGGAATCAAA AATCCGAATC 
CTGGTTGGAA GCTTGGAGAA GAATGAATTT ATTACACTGG CTCATGTGAA TCCCCAGTCA 
TTTCCAGCAC CCAAAGAAAA TCCCGACAAG GAAGAATTTC GCACGATGTG GGTGATTGGG 
TTAGTGTTTA AAAAAACAGA AAACTCTGAA AACCTCAGTG TTGATCTCAC CTATGATATT 
CAGTCTTTCA CAGATACAGT TTATAGGCAA GCAATAAACA GCAAGATGTT TGAGGTGGAT 
ATGAAAATTG CTGCAATGCA TGTAAAAAGA AAGCAACTCC ATCAACTACT ACCTAATCAT 
GTGCTTCAGA AAAAGAAAAA GCATTCAACA GAAGGTGTCA AATTGACAGC TCTCAATGAC 
AGCAGCCTCG ACTTGTCTAT GGACAGTGAT AACAGCATGT CTGTGCCTTC ACCTACTAGT 
GCTACGAAGA CCAGTCCATT GAACAGTTCT GGCAGCTCTC AGGGCAGAAA CAGTCCTGCT 
CCAGCTGTAA CAGCAGCATC TGTGACCAAC ATACAGGCTA CTGAAGTTTC TGTGCCACAA 
GTAAATTCCA GTGAAAGCTC AGGGGGTACA TCGAGTGAAA GCATTCCTCA AACTGCCACA 
CAACCAGCCA TTTCTCCACC ACCAAAGCCT ACGGTCTCCA GAGTTGTTTC TTCAACACGT 
CTGGTAAACC CACCACCTAG ATCTTCAGGA AATGCAGCAA CTTCAGGAAA TGCAGCAACA 
AAAATACCTA CTCCTATAGT AGGAGTCAAG AGGACATCCT CACCTCATAA AGAAGAGAGT 
CCCAAGAAAA CCAAAACAGA AGAGGAACAA CTTGATACAG AGACAAGTAC AACTCAATCA 
GAAACTATTC AGACAGCGGC TTCTCTGTTG GCCTCTCAGA AAACATCCAG TACAGACCTT 
TCTGATATCC CTGCTCTCCC TGCAAATCCT ATTCCTGTTA TCAAGAATTC AATAAAACTG 
AGATTGAATC GGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PAPOLA ( XM_005267282.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_005267339.1 Homo sapiens poly(A) polymerase alpha (PAPOLA), transcript variant X3, mRNA.

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CloneID	OHu47486
Clone ID Related Accession (Same CDS sequence)	NM_001363665.1 , NM_001363665.2 , XM_005267282.2
Accession Version	XM_005267282.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2100bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	poly(A) polymerase alpha isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005267282.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGTTTC CAGTTACAAC ACAGGGATCA CAACAAACAC AACCGCCACA GAAGCACTAT 
GGCATTACTT CTCCTATCAG CTTAGCAGCC CCCAAGGAGA CTGACTGCGT ACTTACACAG 
AAACTAATTG AGACATTGAA ACCCTTTGGG GTTTTTGAAG AGGAAGAGGA ACTGCAGCGC 
AGGATTTTAA TTTTGGGAAA ACTAAATAAC CTGGTAAAAG AGTGGATACG AGAAATCAGT 
GAAAGCAAGA ATCTTCCACA ATCTGTAATT GAAAATGTTG GAGGAAAAAT TTTTACATTT 
GGATCTTACA GATTAGGAGT GCATACAAAA GGTGCTGATA TTGATGCGTT GTGTGTTGCA 
CCAAGACATG TTGATCGAAG TGACTTTTTC ACCTCATTCT ATGATAAGTT GAAATTACAG 
GAAGAAGTAA AAGATTTAAG AGCTGTTGAA GAGGCATTCG TACCAGTTAT TAAACTCTGT 
TTTGATGGGA TAGAGATTGA TATTTTGTTT GCAAGATTAG CACTGCAGAC AATTCCTGAA 
GATTTGGATC TACGAGATGA CAGTCTGCTA AAAAATTTAG ATATAAGATG TATAAGAAGT 
CTTAACGGTT GCAGGGTAAC CGATGAAATT TTACATCTAG TACCAAACAT TGACAACTTC 
AGGTTAACTC TGAGAGCTAT CAAACTATGG GCCAAACGCC ACAACATCTA TTCCAATATA 
TTAGGTTTCC TCGGTGGTGT TTCCTGGGCT ATGCTAGTAG CAAGAACTTG CCAGCTTTAT 
CCAAATGCAA TAGCATCAAC TCTTGTACAT AAATTTTTCT TGGTATTTTC TAAATGGGAA 
TGGCCAAATC CAGTGCTATT GAAACAGCCT GAAGAATGCA ATCTTAATTT GCCTGTATGG 
GACCCAAGGG TAAACCCCAG TGATAGGTAC CATCTTATGC CTATAATTAC ACCAGCATAC 
CCACAACAGA ACTCCACGTA CAATGTGTCC GTTTCAACAC GGATGGTCAT GGTTGAGGAG 
TTTAAACAAG GTCTTGCTAT CACAGATGAA ATTTTGCTGA GTAAGGCAGA GTGGTCCAAA 
CTTTTTGAAG CTCCAAACTT CTTTCAAAAG TACAAGCATT ATATTGTACT TCTAGCAAGT 
GCACCAACAG AAAAACAACG CCTGGAATGG GTGGGCTTGG TGGAATCAAA AATCCGAATC 
CTGGTTGGAA GCTTGGAGAA GAATGAATTT ATTACACTGG CTCATGTGAA TCCCCAGTCA 
TTTCCAGCAC CCAAAGAAAA TCCCGACAAG GAAGAATTTC GCACGATGTG GGTGATTGGG 
TTAGTGTTTA AAAAAACAGA AAACTCTGAA AACCTCAGTG TTGATCTCAC CTATGATATT 
CAGTCTTTCA CAGATACAGT TTATAGGCAA GCAATAAACA GCAAGATGTT TGAGGTGGAT 
ATGAAAATTG CTGCAATGCA TGTAAAAAGA AAGCAACTCC ATCAACTACT ACCTAATCAT 
GTGCTTCAGA AAAAGAAAAA GCATTCAACA GAAGGTGTCA AATTGACAGC TCTCAATGAC 
AGCAGCCTCG ACTTGTCTAT GGACAGTGAT AACAGCATGT CTGTGCCTTC ACCTACTAGT 
GCTACGAAGA CCAGTCCATT GAACAGTTCT GGCAGCTCTC AGGGCAGAAA CAGTCCTGCT 
CCAGCTGTAA CAGCAGCATC TGTGACCAAC ATACAGGCTA CTGAAGTTTC TGTGCCACAA 
GTAAATTCCA GTGAAAGCTC AGGGGGTACA TCGAGTGAAA GCATTCCTCA AACTGCCACA 
CAACCAGCCA TTTCTCCACC ACCAAAGCCT ACGGTCTCCA GAGTTGTTTC TTCAACACGT 
CTGGTAAACC CACCACCTAG ATCTTCAGGA AATGCAGCAA CTTCAGGAAA TGCAGCAACA 
AAAATACCTA CTCCTATAGT AGGAGTCAAG AGGACATCCT CACCTCATAA AGAAGAGAGT 
CCCAAGAAAA CCAAAACAGA AGAGAAAACA TCCAGTACAG ACCTTTCTGA TATCCCTGCT 
CTCCCTGCAA ATCCTATTCC TGTTATCAAG AATTCAATAA AACTGAGATT GAATCGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005267339.1
CDS	214..2313
Translation	MPFPVTTQGSQQTQPPQKHYGITSPISLAAPKETDCVLTQKLIETLKPFGVFEEEEELQRRILILGKLNNLVKEWIREISESKNLPQSVIENVGGKIFTFGSYRLGVHTKGADIDALCVAPRHVDRSDFFTSFYDKLKLQEEVKDLRAVEEAFVPVIKLCFDGIEIDILFARLALQTIPEDLDLRDDSLLKNLDIRCIRSLNGCRVTDEILHLVPNIDNFRLTLRAIKLWAKRHNIYSNILGFLGGVSWAMLVARTCQLYPNAIASTLVHKFFLVFSKWEWPNPVLLKQPEECNLNLPVWDPRVNPSDRYHLMPIITPAYPQQNSTYNVSVSTRMVMVEEFKQGLAITDEILLSKAEWSKLFEAPNFFQKYKHYIVLLASAPTEKQRLEWVGLVESKIRILVGSLEKNEFITLAHVNPQSFPAPKENPDKEEFRTMWVIGLVFKKTENSENLSVDLTYDIQSFTDTVYRQAINSKMFEVDMKIAAMHVKRKQLHQLLPNHVLQKKKKHSTEGVKLTALNDSSLDLSMDSDNSMSVPSPTSATKTSPLNSSGSSQGRNSPAPAVTAASVTNIQATEVSVPQVNSSESSGGTSSESIPQTATQPAISPPPKPTVSRVVSSTRLVNPPPRSSGNAATSGNAATKIPTPIVGVKRTSSPHKEESPKKTKTEEKTSSTDLSDIPALPANPIPVIKNSIKLRLNR

Target ORF information:

RefSeq Version	XM_005267282.2
Organism	Homo sapiens(human)
Definition	Homo sapiens poly(A) polymerase alpha (PAPOLA), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005267282.2

ORF Insert Sequence:

ATGCCGTTTC CAGTTACAAC ACAGGGATCA CAACAAACAC AACCGCCACA GAAGCACTAT 
GGCATTACTT CTCCTATCAG CTTAGCAGCC CCCAAGGAGA CTGACTGCGT ACTTACACAG 
AAACTAATTG AGACATTGAA ACCCTTTGGG GTTTTTGAAG AGGAAGAGGA ACTGCAGCGC 
AGGATTTTAA TTTTGGGAAA ACTAAATAAC CTGGTAAAAG AGTGGATACG AGAAATCAGT 
GAAAGCAAGA ATCTTCCACA ATCTGTAATT GAAAATGTTG GAGGAAAAAT TTTTACATTT 
GGATCTTACA GATTAGGAGT GCATACAAAA GGTGCTGATA TTGATGCGTT GTGTGTTGCA 
CCAAGACATG TTGATCGAAG TGACTTTTTC ACCTCATTCT ATGATAAGTT GAAATTACAG 
GAAGAAGTAA AAGATTTAAG AGCTGTTGAA GAGGCATTCG TACCAGTTAT TAAACTCTGT 
TTTGATGGGA TAGAGATTGA TATTTTGTTT GCAAGATTAG CACTGCAGAC AATTCCTGAA 
GATTTGGATC TACGAGATGA CAGTCTGCTA AAAAATTTAG ATATAAGATG TATAAGAAGT 
CTTAACGGTT GCAGGGTAAC CGATGAAATT TTACATCTAG TACCAAACAT TGACAACTTC 
AGGTTAACTC TGAGAGCTAT CAAACTATGG GCCAAACGCC ACAACATCTA TTCCAATATA 
TTAGGTTTCC TCGGTGGTGT TTCCTGGGCT ATGCTAGTAG CAAGAACTTG CCAGCTTTAT 
CCAAATGCAA TAGCATCAAC TCTTGTACAT AAATTTTTCT TGGTATTTTC TAAATGGGAA 
TGGCCAAATC CAGTGCTATT GAAACAGCCT GAAGAATGCA ATCTTAATTT GCCTGTATGG 
GACCCAAGGG TAAACCCCAG TGATAGGTAC CATCTTATGC CTATAATTAC ACCAGCATAC 
CCACAACAGA ACTCCACGTA CAATGTGTCC GTTTCAACAC GGATGGTCAT GGTTGAGGAG 
TTTAAACAAG GTCTTGCTAT CACAGATGAA ATTTTGCTGA GTAAGGCAGA GTGGTCCAAA 
CTTTTTGAAG CTCCAAACTT CTTTCAAAAG TACAAGCATT ATATTGTACT TCTAGCAAGT 
GCACCAACAG AAAAACAACG CCTGGAATGG GTGGGCTTGG TGGAATCAAA AATCCGAATC 
CTGGTTGGAA GCTTGGAGAA GAATGAATTT ATTACACTGG CTCATGTGAA TCCCCAGTCA 
TTTCCAGCAC CCAAAGAAAA TCCCGACAAG GAAGAATTTC GCACGATGTG GGTGATTGGG 
TTAGTGTTTA AAAAAACAGA AAACTCTGAA AACCTCAGTG TTGATCTCAC CTATGATATT 
CAGTCTTTCA CAGATACAGT TTATAGGCAA GCAATAAACA GCAAGATGTT TGAGGTGGAT 
ATGAAAATTG CTGCAATGCA TGTAAAAAGA AAGCAACTCC ATCAACTACT ACCTAATCAT 
GTGCTTCAGA AAAAGAAAAA GCATTCAACA GAAGGTGTCA AATTGACAGC TCTCAATGAC 
AGCAGCCTCG ACTTGTCTAT GGACAGTGAT AACAGCATGT CTGTGCCTTC ACCTACTAGT 
GCTACGAAGA CCAGTCCATT GAACAGTTCT GGCAGCTCTC AGGGCAGAAA CAGTCCTGCT 
CCAGCTGTAA CAGCAGCATC TGTGACCAAC ATACAGGCTA CTGAAGTTTC TGTGCCACAA 
GTAAATTCCA GTGAAAGCTC AGGGGGTACA TCGAGTGAAA GCATTCCTCA AACTGCCACA 
CAACCAGCCA TTTCTCCACC ACCAAAGCCT ACGGTCTCCA GAGTTGTTTC TTCAACACGT 
CTGGTAAACC CACCACCTAG ATCTTCAGGA AATGCAGCAA CTTCAGGAAA TGCAGCAACA 
AAAATACCTA CTCCTATAGT AGGAGTCAAG AGGACATCCT CACCTCATAA AGAAGAGAGT 
CCCAAGAAAA CCAAAACAGA AGAGAAAACA TCCAGTACAG ACCTTTCTGA TATCCCTGCT 
CTCCCTGCAA ATCCTATTCC TGTTATCAAG AATTCAATAA AACTGAGATT GAATCGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PAPOLA ( NM_001363664.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001350593.1 Homo sapiens poly(A) polymerase alpha (PAPOLA), transcript variant 8, mRNA.

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CloneID	OHu84741
Clone ID Related Accession (Same CDS sequence)	NM_001363664.1 , XM_017020935.1 , NM_001363664.2
Accession Version	NM_001363664.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2172bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-30
Organism	Homo sapiens(human)
Product	poly(A) polymerase alpha isoform 7
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL163051.5. On May 29, 2018 this sequence version replaced XM_017020935.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.130884.1, SRR1660807.85467.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END##

ATGCCGTTTC CAGTTACAAC ACAGGGATCA CAACAAACAC AACCGCCACA GAAGCACTAT 
GGCATTACTT CTCCTATCAG CTTAGCAGCC CCCAAGGAGA CTGACTGCGT ACTTACACAG 
AAACTAATTG AGACATTGAA ACCCTTTGGG GTTTTTGAAG AGGAAGAGGA ACTGCAGCGC 
AGGATTTTAA TTTTGGGAAA ACTAAATAAC CTGGTAAAAG AGTGGATACG AGAAATCAGT 
GAAAGCAAGA ATCTTCCACA ATCTGTAATT GAAAATGTTG GAGGAAAAAT TTTTACATTT 
GGATCTTACA GATTAGGAGT GCATACAAAA GGTGCTGATA TTGATGCGTT GTGTGTTGCA 
CCAAGACATG TTGATCGAAG TGACTTTTTC ACCTCATTCT ATGATAAGTT GAAATTACAG 
GAAGAAGTAA AAGATTTAAG AGCTGTTGAA GAGGCATTCG TACCAGTTAT TAAACTCTGT 
TTTGATGGGA TAGAGATTGA TATTTTGTTT GCAAGATTAG CACTGCAGAC AATTCCTGAA 
GATTTGGATC TACGAGATGA CAGTCTGCTA AAAAATTTAG ATATAAGATG TATAAGAAGT 
CTTAACGGTT GCAGGGTAAC CGATGAAATT TTACATCTAG TACCAAACAT TGACAACTTC 
AGGTTAACTC TGAGAGCTAT CAAACTATGG GCCAAACGCC ACAACATCTA TTCCAATATA 
TTAGGTTTCC TCGGTGGTGT TTCCTGGGCT ATGCTAGTAG CAAGAACTTG CCAGCTTTAT 
CCAAATGCAA TAGCATCAAC TCTTGTACAT AAATTTTTCT TGGTATTTTC TAAATGGGAA 
TGGCCAAATC CAGTGCTATT GAAACAGCCT GAAGAATGCA ATCTTAATTT GCCTGTATGG 
GACCCAAGGG TAAACCCCAG TGATAGGTAC CATCTTATGC CTATAATTAC ACCAGCATAC 
CCACAACAGA ACTCCACGTA CAATGTGTCC GTTTCAACAC GGATGGTCAT GGTTGAGGAG 
TTTAAACAAG GTCTTGCTAT CACAGATGAA ATTTTGCTGA GTAAGGCAGA GTGGTCCAAA 
CTTTTTGAAG CTCCAAACTT CTTTCAAAAG TACAAGCATT ATATTGTACT TCTAGCAAGT 
GCACCAACAG AAAAACAACG CCTGGAATGG GTGGGCTTGG TGGAATCAAA AATCCGAATC 
CTGGTTGGAA GCTTGGAGAA GAATGAATTT ATTACACTGG CTCATGTGAA TCCCCAGTCA 
TTTCCAGCAC CCAAAGAAAA TCCCGACAAG GAAGAATTTC GCACGATGTG GGTGATTGGG 
TTAGTGTTTA AAAAAACAGA AAACTCTGAA AACCTCAGTG TTGATCTCAC CTATGATATT 
CAGTCTTTCA CAGATACAGT TTATAGGCAA GCAATAAACA GCAAGATGTT TGAGGTGGAT 
ATGAAAATTG CTGCAATGCA TGTAAAAAGA AAGCAACTCC ATCAACTACT ACCTAATCAT 
GTGCTTCAGA AAAAGAAAAA GCATTCAACA GAAGGTGTCA AATTGACAGC TCTCAATGAC 
AGCAGCCTCG ACTTGTCTAT GGACAGTGAT AACAGCATGT CTGTGCCTTC ACCTACTAGT 
GCTACGAAGA CCAGTCCATT GAACAGTTCT GGCAGCTCTC AGGGAAACAG TCCTGCTCCA 
GCTGTAACAG CAGCATCTGT GACCAACATA CAGGCTACTG AAGTTTCTGT GCCACAAGTA 
AATTCCAGTG AAAGCTCAGG GGGTACATCG AGTGAAAGCA TTCCTCAAAC TGCCACACAA 
CCAGCCATTT CTCCACCACC AAAGCCTACG GTCTCCAGAG TTGTTTCTTC AACACGTCTG 
GTAAACCCAC CACCTAGATC TTCAGGAAAT GCAGCAACTT CAGGAAATGC AGCAACAAAA 
ATACCTACTC CTATAGTAGG AGTCAAGAGG ACATCCTCAC CTCATAAAGA AGAGAGTCCC 
AAGAAAACCA AAACAGAAGA GGAACAACTT GATACAGAGA CAAGTACAAC TCAATCAGAA 
ACTATTCAGA CAGCGGCTTC TCTGTTGGCC TCTCAGAAAA CATCCAGTAC AGACCTTTCT 
GATATCCCTG CTCTCCCTGC AAATCCTATT CCTGTTATCA AGAATTCAAT AAAACTGAGA 
TTGAATCGGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001350593.1
CDS	218..2389
Translation	MPFPVTTQGSQQTQPPQKHYGITSPISLAAPKETDCVLTQKLIETLKPFGVFEEEEELQRRILILGKLNNLVKEWIREISESKNLPQSVIENVGGKIFTFGSYRLGVHTKGADIDALCVAPRHVDRSDFFTSFYDKLKLQEEVKDLRAVEEAFVPVIKLCFDGIEIDILFARLALQTIPEDLDLRDDSLLKNLDIRCIRSLNGCRVTDEILHLVPNIDNFRLTLRAIKLWAKRHNIYSNILGFLGGVSWAMLVARTCQLYPNAIASTLVHKFFLVFSKWEWPNPVLLKQPEECNLNLPVWDPRVNPSDRYHLMPIITPAYPQQNSTYNVSVSTRMVMVEEFKQGLAITDEILLSKAEWSKLFEAPNFFQKYKHYIVLLASAPTEKQRLEWVGLVESKIRILVGSLEKNEFITLAHVNPQSFPAPKENPDKEEFRTMWVIGLVFKKTENSENLSVDLTYDIQSFTDTVYRQAINSKMFEVDMKIAAMHVKRKQLHQLLPNHVLQKKKKHSTEGVKLTALNDSSLDLSMDSDNSMSVPSPTSATKTSPLNSSGSSQGNSPAPAVTAASVTNIQATEVSVPQVNSSESSGGTSSESIPQTATQPAISPPPKPTVSRVVSSTRLVNPPPRSSGNAATSGNAATKIPTPIVGVKRTSSPHKEESPKKTKTEEEQLDTETSTTQSETIQTAASLLASQKTSSTDLSDIPALPANPIPVIKNSIKLRLNR

Target ORF information:

RefSeq Version	NM_001363664.1
Organism	Homo sapiens(human)
Definition	Homo sapiens poly(A) polymerase alpha (PAPOLA), transcript variant 8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001363664.1

ORF Insert Sequence:

ATGCCGTTTC CAGTTACAAC ACAGGGATCA CAACAAACAC AACCGCCACA GAAGCACTAT 
GGCATTACTT CTCCTATCAG CTTAGCAGCC CCCAAGGAGA CTGACTGCGT ACTTACACAG 
AAACTAATTG AGACATTGAA ACCCTTTGGG GTTTTTGAAG AGGAAGAGGA ACTGCAGCGC 
AGGATTTTAA TTTTGGGAAA ACTAAATAAC CTGGTAAAAG AGTGGATACG AGAAATCAGT 
GAAAGCAAGA ATCTTCCACA ATCTGTAATT GAAAATGTTG GAGGAAAAAT TTTTACATTT 
GGATCTTACA GATTAGGAGT GCATACAAAA GGTGCTGATA TTGATGCGTT GTGTGTTGCA 
CCAAGACATG TTGATCGAAG TGACTTTTTC ACCTCATTCT ATGATAAGTT GAAATTACAG 
GAAGAAGTAA AAGATTTAAG AGCTGTTGAA GAGGCATTCG TACCAGTTAT TAAACTCTGT 
TTTGATGGGA TAGAGATTGA TATTTTGTTT GCAAGATTAG CACTGCAGAC AATTCCTGAA 
GATTTGGATC TACGAGATGA CAGTCTGCTA AAAAATTTAG ATATAAGATG TATAAGAAGT 
CTTAACGGTT GCAGGGTAAC CGATGAAATT TTACATCTAG TACCAAACAT TGACAACTTC 
AGGTTAACTC TGAGAGCTAT CAAACTATGG GCCAAACGCC ACAACATCTA TTCCAATATA 
TTAGGTTTCC TCGGTGGTGT TTCCTGGGCT ATGCTAGTAG CAAGAACTTG CCAGCTTTAT 
CCAAATGCAA TAGCATCAAC TCTTGTACAT AAATTTTTCT TGGTATTTTC TAAATGGGAA 
TGGCCAAATC CAGTGCTATT GAAACAGCCT GAAGAATGCA ATCTTAATTT GCCTGTATGG 
GACCCAAGGG TAAACCCCAG TGATAGGTAC CATCTTATGC CTATAATTAC ACCAGCATAC 
CCACAACAGA ACTCCACGTA CAATGTGTCC GTTTCAACAC GGATGGTCAT GGTTGAGGAG 
TTTAAACAAG GTCTTGCTAT CACAGATGAA ATTTTGCTGA GTAAGGCAGA GTGGTCCAAA 
CTTTTTGAAG CTCCAAACTT CTTTCAAAAG TACAAGCATT ATATTGTACT TCTAGCAAGT 
GCACCAACAG AAAAACAACG CCTGGAATGG GTGGGCTTGG TGGAATCAAA AATCCGAATC 
CTGGTTGGAA GCTTGGAGAA GAATGAATTT ATTACACTGG CTCATGTGAA TCCCCAGTCA 
TTTCCAGCAC CCAAAGAAAA TCCCGACAAG GAAGAATTTC GCACGATGTG GGTGATTGGG 
TTAGTGTTTA AAAAAACAGA AAACTCTGAA AACCTCAGTG TTGATCTCAC CTATGATATT 
CAGTCTTTCA CAGATACAGT TTATAGGCAA GCAATAAACA GCAAGATGTT TGAGGTGGAT 
ATGAAAATTG CTGCAATGCA TGTAAAAAGA AAGCAACTCC ATCAACTACT ACCTAATCAT 
GTGCTTCAGA AAAAGAAAAA GCATTCAACA GAAGGTGTCA AATTGACAGC TCTCAATGAC 
AGCAGCCTCG ACTTGTCTAT GGACAGTGAT AACAGCATGT CTGTGCCTTC ACCTACTAGT 
GCTACGAAGA CCAGTCCATT GAACAGTTCT GGCAGCTCTC AGGGAAACAG TCCTGCTCCA 
GCTGTAACAG CAGCATCTGT GACCAACATA CAGGCTACTG AAGTTTCTGT GCCACAAGTA 
AATTCCAGTG AAAGCTCAGG GGGTACATCG AGTGAAAGCA TTCCTCAAAC TGCCACACAA 
CCAGCCATTT CTCCACCACC AAAGCCTACG GTCTCCAGAG TTGTTTCTTC AACACGTCTG 
GTAAACCCAC CACCTAGATC TTCAGGAAAT GCAGCAACTT CAGGAAATGC AGCAACAAAA 
ATACCTACTC CTATAGTAGG AGTCAAGAGG ACATCCTCAC CTCATAAAGA AGAGAGTCCC 
AAGAAAACCA AAACAGAAGA GGAACAACTT GATACAGAGA CAAGTACAAC TCAATCAGAA 
ACTATTCAGA CAGCGGCTTC TCTGTTGGCC TCTCAGAAAA CATCCAGTAC AGACCTTTCT 
GATATCCCTG CTCTCCCTGC AAATCCTATT CCTGTTATCA AGAATTCAAT AAAACTGAGA 
TTGAATCGGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PAPOLA ( NM_001363664.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001350593.1 Homo sapiens poly(A) polymerase alpha (PAPOLA), transcript variant 8, mRNA.

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CloneID	OHu84741
Clone ID Related Accession (Same CDS sequence)	NM_001363664.1 , XM_017020935.1 , NM_001363664.2
Accession Version	NM_001363664.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2172bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-06
Organism	Homo sapiens(human)
Product	poly(A) polymerase alpha isoform 7
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL163051.5. On Jun 2, 2019 this sequence version replaced NM_001363664.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.130884.1, SRR1660807.85467.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END##

ATGCCGTTTC CAGTTACAAC ACAGGGATCA CAACAAACAC AACCGCCACA GAAGCACTAT 
GGCATTACTT CTCCTATCAG CTTAGCAGCC CCCAAGGAGA CTGACTGCGT ACTTACACAG 
AAACTAATTG AGACATTGAA ACCCTTTGGG GTTTTTGAAG AGGAAGAGGA ACTGCAGCGC 
AGGATTTTAA TTTTGGGAAA ACTAAATAAC CTGGTAAAAG AGTGGATACG AGAAATCAGT 
GAAAGCAAGA ATCTTCCACA ATCTGTAATT GAAAATGTTG GAGGAAAAAT TTTTACATTT 
GGATCTTACA GATTAGGAGT GCATACAAAA GGTGCTGATA TTGATGCGTT GTGTGTTGCA 
CCAAGACATG TTGATCGAAG TGACTTTTTC ACCTCATTCT ATGATAAGTT GAAATTACAG 
GAAGAAGTAA AAGATTTAAG AGCTGTTGAA GAGGCATTCG TACCAGTTAT TAAACTCTGT 
TTTGATGGGA TAGAGATTGA TATTTTGTTT GCAAGATTAG CACTGCAGAC AATTCCTGAA 
GATTTGGATC TACGAGATGA CAGTCTGCTA AAAAATTTAG ATATAAGATG TATAAGAAGT 
CTTAACGGTT GCAGGGTAAC CGATGAAATT TTACATCTAG TACCAAACAT TGACAACTTC 
AGGTTAACTC TGAGAGCTAT CAAACTATGG GCCAAACGCC ACAACATCTA TTCCAATATA 
TTAGGTTTCC TCGGTGGTGT TTCCTGGGCT ATGCTAGTAG CAAGAACTTG CCAGCTTTAT 
CCAAATGCAA TAGCATCAAC TCTTGTACAT AAATTTTTCT TGGTATTTTC TAAATGGGAA 
TGGCCAAATC CAGTGCTATT GAAACAGCCT GAAGAATGCA ATCTTAATTT GCCTGTATGG 
GACCCAAGGG TAAACCCCAG TGATAGGTAC CATCTTATGC CTATAATTAC ACCAGCATAC 
CCACAACAGA ACTCCACGTA CAATGTGTCC GTTTCAACAC GGATGGTCAT GGTTGAGGAG 
TTTAAACAAG GTCTTGCTAT CACAGATGAA ATTTTGCTGA GTAAGGCAGA GTGGTCCAAA 
CTTTTTGAAG CTCCAAACTT CTTTCAAAAG TACAAGCATT ATATTGTACT TCTAGCAAGT 
GCACCAACAG AAAAACAACG CCTGGAATGG GTGGGCTTGG TGGAATCAAA AATCCGAATC 
CTGGTTGGAA GCTTGGAGAA GAATGAATTT ATTACACTGG CTCATGTGAA TCCCCAGTCA 
TTTCCAGCAC CCAAAGAAAA TCCCGACAAG GAAGAATTTC GCACGATGTG GGTGATTGGG 
TTAGTGTTTA AAAAAACAGA AAACTCTGAA AACCTCAGTG TTGATCTCAC CTATGATATT 
CAGTCTTTCA CAGATACAGT TTATAGGCAA GCAATAAACA GCAAGATGTT TGAGGTGGAT 
ATGAAAATTG CTGCAATGCA TGTAAAAAGA AAGCAACTCC ATCAACTACT ACCTAATCAT 
GTGCTTCAGA AAAAGAAAAA GCATTCAACA GAAGGTGTCA AATTGACAGC TCTCAATGAC 
AGCAGCCTCG ACTTGTCTAT GGACAGTGAT AACAGCATGT CTGTGCCTTC ACCTACTAGT 
GCTACGAAGA CCAGTCCATT GAACAGTTCT GGCAGCTCTC AGGGAAACAG TCCTGCTCCA 
GCTGTAACAG CAGCATCTGT GACCAACATA CAGGCTACTG AAGTTTCTGT GCCACAAGTA 
AATTCCAGTG AAAGCTCAGG GGGTACATCG AGTGAAAGCA TTCCTCAAAC TGCCACACAA 
CCAGCCATTT CTCCACCACC AAAGCCTACG GTCTCCAGAG TTGTTTCTTC AACACGTCTG 
GTAAACCCAC CACCTAGATC TTCAGGAAAT GCAGCAACTT CAGGAAATGC AGCAACAAAA 
ATACCTACTC CTATAGTAGG AGTCAAGAGG ACATCCTCAC CTCATAAAGA AGAGAGTCCC 
AAGAAAACCA AAACAGAAGA GGAACAACTT GATACAGAGA CAAGTACAAC TCAATCAGAA 
ACTATTCAGA CAGCGGCTTC TCTGTTGGCC TCTCAGAAAA CATCCAGTAC AGACCTTTCT 
GATATCCCTG CTCTCCCTGC AAATCCTATT CCTGTTATCA AGAATTCAAT AAAACTGAGA 
TTGAATCGGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001350593.1
CDS	212..2383
Translation	MPFPVTTQGSQQTQPPQKHYGITSPISLAAPKETDCVLTQKLIETLKPFGVFEEEEELQRRILILGKLNNLVKEWIREISESKNLPQSVIENVGGKIFTFGSYRLGVHTKGADIDALCVAPRHVDRSDFFTSFYDKLKLQEEVKDLRAVEEAFVPVIKLCFDGIEIDILFARLALQTIPEDLDLRDDSLLKNLDIRCIRSLNGCRVTDEILHLVPNIDNFRLTLRAIKLWAKRHNIYSNILGFLGGVSWAMLVARTCQLYPNAIASTLVHKFFLVFSKWEWPNPVLLKQPEECNLNLPVWDPRVNPSDRYHLMPIITPAYPQQNSTYNVSVSTRMVMVEEFKQGLAITDEILLSKAEWSKLFEAPNFFQKYKHYIVLLASAPTEKQRLEWVGLVESKIRILVGSLEKNEFITLAHVNPQSFPAPKENPDKEEFRTMWVIGLVFKKTENSENLSVDLTYDIQSFTDTVYRQAINSKMFEVDMKIAAMHVKRKQLHQLLPNHVLQKKKKHSTEGVKLTALNDSSLDLSMDSDNSMSVPSPTSATKTSPLNSSGSSQGNSPAPAVTAASVTNIQATEVSVPQVNSSESSGGTSSESIPQTATQPAISPPPKPTVSRVVSSTRLVNPPPRSSGNAATSGNAATKIPTPIVGVKRTSSPHKEESPKKTKTEEEQLDTETSTTQSETIQTAASLLASQKTSSTDLSDIPALPANPIPVIKNSIKLRLNR

Target ORF information:

RefSeq Version	NM_001363664.2
Organism	Homo sapiens(human)
Definition	Homo sapiens poly(A) polymerase alpha (PAPOLA), transcript variant 8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001363664.2

ORF Insert Sequence:

ATGCCGTTTC CAGTTACAAC ACAGGGATCA CAACAAACAC AACCGCCACA GAAGCACTAT 
GGCATTACTT CTCCTATCAG CTTAGCAGCC CCCAAGGAGA CTGACTGCGT ACTTACACAG 
AAACTAATTG AGACATTGAA ACCCTTTGGG GTTTTTGAAG AGGAAGAGGA ACTGCAGCGC 
AGGATTTTAA TTTTGGGAAA ACTAAATAAC CTGGTAAAAG AGTGGATACG AGAAATCAGT 
GAAAGCAAGA ATCTTCCACA ATCTGTAATT GAAAATGTTG GAGGAAAAAT TTTTACATTT 
GGATCTTACA GATTAGGAGT GCATACAAAA GGTGCTGATA TTGATGCGTT GTGTGTTGCA 
CCAAGACATG TTGATCGAAG TGACTTTTTC ACCTCATTCT ATGATAAGTT GAAATTACAG 
GAAGAAGTAA AAGATTTAAG AGCTGTTGAA GAGGCATTCG TACCAGTTAT TAAACTCTGT 
TTTGATGGGA TAGAGATTGA TATTTTGTTT GCAAGATTAG CACTGCAGAC AATTCCTGAA 
GATTTGGATC TACGAGATGA CAGTCTGCTA AAAAATTTAG ATATAAGATG TATAAGAAGT 
CTTAACGGTT GCAGGGTAAC CGATGAAATT TTACATCTAG TACCAAACAT TGACAACTTC 
AGGTTAACTC TGAGAGCTAT CAAACTATGG GCCAAACGCC ACAACATCTA TTCCAATATA 
TTAGGTTTCC TCGGTGGTGT TTCCTGGGCT ATGCTAGTAG CAAGAACTTG CCAGCTTTAT 
CCAAATGCAA TAGCATCAAC TCTTGTACAT AAATTTTTCT TGGTATTTTC TAAATGGGAA 
TGGCCAAATC CAGTGCTATT GAAACAGCCT GAAGAATGCA ATCTTAATTT GCCTGTATGG 
GACCCAAGGG TAAACCCCAG TGATAGGTAC CATCTTATGC CTATAATTAC ACCAGCATAC 
CCACAACAGA ACTCCACGTA CAATGTGTCC GTTTCAACAC GGATGGTCAT GGTTGAGGAG 
TTTAAACAAG GTCTTGCTAT CACAGATGAA ATTTTGCTGA GTAAGGCAGA GTGGTCCAAA 
CTTTTTGAAG CTCCAAACTT CTTTCAAAAG TACAAGCATT ATATTGTACT TCTAGCAAGT 
GCACCAACAG AAAAACAACG CCTGGAATGG GTGGGCTTGG TGGAATCAAA AATCCGAATC 
CTGGTTGGAA GCTTGGAGAA GAATGAATTT ATTACACTGG CTCATGTGAA TCCCCAGTCA 
TTTCCAGCAC CCAAAGAAAA TCCCGACAAG GAAGAATTTC GCACGATGTG GGTGATTGGG 
TTAGTGTTTA AAAAAACAGA AAACTCTGAA AACCTCAGTG TTGATCTCAC CTATGATATT 
CAGTCTTTCA CAGATACAGT TTATAGGCAA GCAATAAACA GCAAGATGTT TGAGGTGGAT 
ATGAAAATTG CTGCAATGCA TGTAAAAAGA AAGCAACTCC ATCAACTACT ACCTAATCAT 
GTGCTTCAGA AAAAGAAAAA GCATTCAACA GAAGGTGTCA AATTGACAGC TCTCAATGAC 
AGCAGCCTCG ACTTGTCTAT GGACAGTGAT AACAGCATGT CTGTGCCTTC ACCTACTAGT 
GCTACGAAGA CCAGTCCATT GAACAGTTCT GGCAGCTCTC AGGGAAACAG TCCTGCTCCA 
GCTGTAACAG CAGCATCTGT GACCAACATA CAGGCTACTG AAGTTTCTGT GCCACAAGTA 
AATTCCAGTG AAAGCTCAGG GGGTACATCG AGTGAAAGCA TTCCTCAAAC TGCCACACAA 
CCAGCCATTT CTCCACCACC AAAGCCTACG GTCTCCAGAG TTGTTTCTTC AACACGTCTG 
GTAAACCCAC CACCTAGATC TTCAGGAAAT GCAGCAACTT CAGGAAATGC AGCAACAAAA 
ATACCTACTC CTATAGTAGG AGTCAAGAGG ACATCCTCAC CTCATAAAGA AGAGAGTCCC 
AAGAAAACCA AAACAGAAGA GGAACAACTT GATACAGAGA CAAGTACAAC TCAATCAGAA 
ACTATTCAGA CAGCGGCTTC TCTGTTGGCC TCTCAGAAAA CATCCAGTAC AGACCTTTCT 
GATATCCCTG CTCTCCCTGC AAATCCTATT CCTGTTATCA AGAATTCAAT AAAACTGAGA 
TTGAATCGGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PAPOLA ( NM_001363666.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001350595.1 Homo sapiens poly(A) polymerase alpha (PAPOLA), transcript variant 10, mRNA.

Price & Availability↑

CloneID	OHu84742
Clone ID Related Accession (Same CDS sequence)	NM_001363666.1 , XM_017020936.1 , NM_001363666.2
Accession Version	NM_001363666.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2097bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-30
Organism	Homo sapiens(human)
Product	poly(A) polymerase alpha isoform 9
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL163051.5. On May 29, 2018 this sequence version replaced XM_017020936.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.24826.1, SRR1660807.36179.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END##

ATGCCGTTTC CAGTTACAAC ACAGGGATCA CAACAAACAC AACCGCCACA GAAGCACTAT 
GGCATTACTT CTCCTATCAG CTTAGCAGCC CCCAAGGAGA CTGACTGCGT ACTTACACAG 
AAACTAATTG AGACATTGAA ACCCTTTGGG GTTTTTGAAG AGGAAGAGGA ACTGCAGCGC 
AGGATTTTAA TTTTGGGAAA ACTAAATAAC CTGGTAAAAG AGTGGATACG AGAAATCAGT 
GAAAGCAAGA ATCTTCCACA ATCTGTAATT GAAAATGTTG GAGGAAAAAT TTTTACATTT 
GGATCTTACA GATTAGGAGT GCATACAAAA GGTGCTGATA TTGATGCGTT GTGTGTTGCA 
CCAAGACATG TTGATCGAAG TGACTTTTTC ACCTCATTCT ATGATAAGTT GAAATTACAG 
GAAGAAGTAA AAGATTTAAG AGCTGTTGAA GAGGCATTCG TACCAGTTAT TAAACTCTGT 
TTTGATGGGA TAGAGATTGA TATTTTGTTT GCAAGATTAG CACTGCAGAC AATTCCTGAA 
GATTTGGATC TACGAGATGA CAGTCTGCTA AAAAATTTAG ATATAAGATG TATAAGAAGT 
CTTAACGGTT GCAGGGTAAC CGATGAAATT TTACATCTAG TACCAAACAT TGACAACTTC 
AGGTTAACTC TGAGAGCTAT CAAACTATGG GCCAAACGCC ACAACATCTA TTCCAATATA 
TTAGGTTTCC TCGGTGGTGT TTCCTGGGCT ATGCTAGTAG CAAGAACTTG CCAGCTTTAT 
CCAAATGCAA TAGCATCAAC TCTTGTACAT AAATTTTTCT TGGTATTTTC TAAATGGGAA 
TGGCCAAATC CAGTGCTATT GAAACAGCCT GAAGAATGCA ATCTTAATTT GCCTGTATGG 
GACCCAAGGG TAAACCCCAG TGATAGGTAC CATCTTATGC CTATAATTAC ACCAGCATAC 
CCACAACAGA ACTCCACGTA CAATGTGTCC GTTTCAACAC GGATGGTCAT GGTTGAGGAG 
TTTAAACAAG GTCTTGCTAT CACAGATGAA ATTTTGCTGA GTAAGGCAGA GTGGTCCAAA 
CTTTTTGAAG CTCCAAACTT CTTTCAAAAG TACAAGCATT ATATTGTACT TCTAGCAAGT 
GCACCAACAG AAAAACAACG CCTGGAATGG GTGGGCTTGG TGGAATCAAA AATCCGAATC 
CTGGTTGGAA GCTTGGAGAA GAATGAATTT ATTACACTGG CTCATGTGAA TCCCCAGTCA 
TTTCCAGCAC CCAAAGAAAA TCCCGACAAG GAAGAATTTC GCACGATGTG GGTGATTGGG 
TTAGTGTTTA AAAAAACAGA AAACTCTGAA AACCTCAGTG TTGATCTCAC CTATGATATT 
CAGTCTTTCA CAGATACAGT TTATAGGCAA GCAATAAACA GCAAGATGTT TGAGGTGGAT 
ATGAAAATTG CTGCAATGCA TGTAAAAAGA AAGCAACTCC ATCAACTACT ACCTAATCAT 
GTGCTTCAGA AAAAGAAAAA GCATTCAACA GAAGGTGTCA AATTGACAGC TCTCAATGAC 
AGCAGCCTCG ACTTGTCTAT GGACAGTGAT AACAGCATGT CTGTGCCTTC ACCTACTAGT 
GCTACGAAGA CCAGTCCATT GAACAGTTCT GGCAGCTCTC AGGGAAACAG TCCTGCTCCA 
GCTGTAACAG CAGCATCTGT GACCAACATA CAGGCTACTG AAGTTTCTGT GCCACAAGTA 
AATTCCAGTG AAAGCTCAGG GGGTACATCG AGTGAAAGCA TTCCTCAAAC TGCCACACAA 
CCAGCCATTT CTCCACCACC AAAGCCTACG GTCTCCAGAG TTGTTTCTTC AACACGTCTG 
GTAAACCCAC CACCTAGATC TTCAGGAAAT GCAGCAACTT CAGGAAATGC AGCAACAAAA 
ATACCTACTC CTATAGTAGG AGTCAAGAGG ACATCCTCAC CTCATAAAGA AGAGAGTCCC 
AAGAAAACCA AAACAGAAGA GAAAACATCC AGTACAGACC TTTCTGATAT CCCTGCTCTC 
CCTGCAAATC CTATTCCTGT TATCAAGAAT TCAATAAAAC TGAGATTGAA TCGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001350595.1
CDS	218..2314
Translation	MPFPVTTQGSQQTQPPQKHYGITSPISLAAPKETDCVLTQKLIETLKPFGVFEEEEELQRRILILGKLNNLVKEWIREISESKNLPQSVIENVGGKIFTFGSYRLGVHTKGADIDALCVAPRHVDRSDFFTSFYDKLKLQEEVKDLRAVEEAFVPVIKLCFDGIEIDILFARLALQTIPEDLDLRDDSLLKNLDIRCIRSLNGCRVTDEILHLVPNIDNFRLTLRAIKLWAKRHNIYSNILGFLGGVSWAMLVARTCQLYPNAIASTLVHKFFLVFSKWEWPNPVLLKQPEECNLNLPVWDPRVNPSDRYHLMPIITPAYPQQNSTYNVSVSTRMVMVEEFKQGLAITDEILLSKAEWSKLFEAPNFFQKYKHYIVLLASAPTEKQRLEWVGLVESKIRILVGSLEKNEFITLAHVNPQSFPAPKENPDKEEFRTMWVIGLVFKKTENSENLSVDLTYDIQSFTDTVYRQAINSKMFEVDMKIAAMHVKRKQLHQLLPNHVLQKKKKHSTEGVKLTALNDSSLDLSMDSDNSMSVPSPTSATKTSPLNSSGSSQGNSPAPAVTAASVTNIQATEVSVPQVNSSESSGGTSSESIPQTATQPAISPPPKPTVSRVVSSTRLVNPPPRSSGNAATSGNAATKIPTPIVGVKRTSSPHKEESPKKTKTEEKTSSTDLSDIPALPANPIPVIKNSIKLRLNR

Target ORF information:

RefSeq Version	NM_001363666.1
Organism	Homo sapiens(human)
Definition	Homo sapiens poly(A) polymerase alpha (PAPOLA), transcript variant 10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001363666.1

ORF Insert Sequence:

ATGCCGTTTC CAGTTACAAC ACAGGGATCA CAACAAACAC AACCGCCACA GAAGCACTAT 
GGCATTACTT CTCCTATCAG CTTAGCAGCC CCCAAGGAGA CTGACTGCGT ACTTACACAG 
AAACTAATTG AGACATTGAA ACCCTTTGGG GTTTTTGAAG AGGAAGAGGA ACTGCAGCGC 
AGGATTTTAA TTTTGGGAAA ACTAAATAAC CTGGTAAAAG AGTGGATACG AGAAATCAGT 
GAAAGCAAGA ATCTTCCACA ATCTGTAATT GAAAATGTTG GAGGAAAAAT TTTTACATTT 
GGATCTTACA GATTAGGAGT GCATACAAAA GGTGCTGATA TTGATGCGTT GTGTGTTGCA 
CCAAGACATG TTGATCGAAG TGACTTTTTC ACCTCATTCT ATGATAAGTT GAAATTACAG 
GAAGAAGTAA AAGATTTAAG AGCTGTTGAA GAGGCATTCG TACCAGTTAT TAAACTCTGT 
TTTGATGGGA TAGAGATTGA TATTTTGTTT GCAAGATTAG CACTGCAGAC AATTCCTGAA 
GATTTGGATC TACGAGATGA CAGTCTGCTA AAAAATTTAG ATATAAGATG TATAAGAAGT 
CTTAACGGTT GCAGGGTAAC CGATGAAATT TTACATCTAG TACCAAACAT TGACAACTTC 
AGGTTAACTC TGAGAGCTAT CAAACTATGG GCCAAACGCC ACAACATCTA TTCCAATATA 
TTAGGTTTCC TCGGTGGTGT TTCCTGGGCT ATGCTAGTAG CAAGAACTTG CCAGCTTTAT 
CCAAATGCAA TAGCATCAAC TCTTGTACAT AAATTTTTCT TGGTATTTTC TAAATGGGAA 
TGGCCAAATC CAGTGCTATT GAAACAGCCT GAAGAATGCA ATCTTAATTT GCCTGTATGG 
GACCCAAGGG TAAACCCCAG TGATAGGTAC CATCTTATGC CTATAATTAC ACCAGCATAC 
CCACAACAGA ACTCCACGTA CAATGTGTCC GTTTCAACAC GGATGGTCAT GGTTGAGGAG 
TTTAAACAAG GTCTTGCTAT CACAGATGAA ATTTTGCTGA GTAAGGCAGA GTGGTCCAAA 
CTTTTTGAAG CTCCAAACTT CTTTCAAAAG TACAAGCATT ATATTGTACT TCTAGCAAGT 
GCACCAACAG AAAAACAACG CCTGGAATGG GTGGGCTTGG TGGAATCAAA AATCCGAATC 
CTGGTTGGAA GCTTGGAGAA GAATGAATTT ATTACACTGG CTCATGTGAA TCCCCAGTCA 
TTTCCAGCAC CCAAAGAAAA TCCCGACAAG GAAGAATTTC GCACGATGTG GGTGATTGGG 
TTAGTGTTTA AAAAAACAGA AAACTCTGAA AACCTCAGTG TTGATCTCAC CTATGATATT 
CAGTCTTTCA CAGATACAGT TTATAGGCAA GCAATAAACA GCAAGATGTT TGAGGTGGAT 
ATGAAAATTG CTGCAATGCA TGTAAAAAGA AAGCAACTCC ATCAACTACT ACCTAATCAT 
GTGCTTCAGA AAAAGAAAAA GCATTCAACA GAAGGTGTCA AATTGACAGC TCTCAATGAC 
AGCAGCCTCG ACTTGTCTAT GGACAGTGAT AACAGCATGT CTGTGCCTTC ACCTACTAGT 
GCTACGAAGA CCAGTCCATT GAACAGTTCT GGCAGCTCTC AGGGAAACAG TCCTGCTCCA 
GCTGTAACAG CAGCATCTGT GACCAACATA CAGGCTACTG AAGTTTCTGT GCCACAAGTA 
AATTCCAGTG AAAGCTCAGG GGGTACATCG AGTGAAAGCA TTCCTCAAAC TGCCACACAA 
CCAGCCATTT CTCCACCACC AAAGCCTACG GTCTCCAGAG TTGTTTCTTC AACACGTCTG 
GTAAACCCAC CACCTAGATC TTCAGGAAAT GCAGCAACTT CAGGAAATGC AGCAACAAAA 
ATACCTACTC CTATAGTAGG AGTCAAGAGG ACATCCTCAC CTCATAAAGA AGAGAGTCCC 
AAGAAAACCA AAACAGAAGA GAAAACATCC AGTACAGACC TTTCTGATAT CCCTGCTCTC 
CCTGCAAATC CTATTCCTGT TATCAAGAAT TCAATAAAAC TGAGATTGAA TCGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PAPOLA ( NM_001363666.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001350595.1 Homo sapiens poly(A) polymerase alpha (PAPOLA), transcript variant 10, mRNA.

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CloneID	OHu84742
Clone ID Related Accession (Same CDS sequence)	NM_001363666.1 , XM_017020936.1 , NM_001363666.2
Accession Version	NM_001363666.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2097bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-06
Organism	Homo sapiens(human)
Product	poly(A) polymerase alpha isoform 9
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL163051.5. On Jun 2, 2019 this sequence version replaced NM_001363666.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.36179.1, SRR1803613.50200.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END##

ATGCCGTTTC CAGTTACAAC ACAGGGATCA CAACAAACAC AACCGCCACA GAAGCACTAT 
GGCATTACTT CTCCTATCAG CTTAGCAGCC CCCAAGGAGA CTGACTGCGT ACTTACACAG 
AAACTAATTG AGACATTGAA ACCCTTTGGG GTTTTTGAAG AGGAAGAGGA ACTGCAGCGC 
AGGATTTTAA TTTTGGGAAA ACTAAATAAC CTGGTAAAAG AGTGGATACG AGAAATCAGT 
GAAAGCAAGA ATCTTCCACA ATCTGTAATT GAAAATGTTG GAGGAAAAAT TTTTACATTT 
GGATCTTACA GATTAGGAGT GCATACAAAA GGTGCTGATA TTGATGCGTT GTGTGTTGCA 
CCAAGACATG TTGATCGAAG TGACTTTTTC ACCTCATTCT ATGATAAGTT GAAATTACAG 
GAAGAAGTAA AAGATTTAAG AGCTGTTGAA GAGGCATTCG TACCAGTTAT TAAACTCTGT 
TTTGATGGGA TAGAGATTGA TATTTTGTTT GCAAGATTAG CACTGCAGAC AATTCCTGAA 
GATTTGGATC TACGAGATGA CAGTCTGCTA AAAAATTTAG ATATAAGATG TATAAGAAGT 
CTTAACGGTT GCAGGGTAAC CGATGAAATT TTACATCTAG TACCAAACAT TGACAACTTC 
AGGTTAACTC TGAGAGCTAT CAAACTATGG GCCAAACGCC ACAACATCTA TTCCAATATA 
TTAGGTTTCC TCGGTGGTGT TTCCTGGGCT ATGCTAGTAG CAAGAACTTG CCAGCTTTAT 
CCAAATGCAA TAGCATCAAC TCTTGTACAT AAATTTTTCT TGGTATTTTC TAAATGGGAA 
TGGCCAAATC CAGTGCTATT GAAACAGCCT GAAGAATGCA ATCTTAATTT GCCTGTATGG 
GACCCAAGGG TAAACCCCAG TGATAGGTAC CATCTTATGC CTATAATTAC ACCAGCATAC 
CCACAACAGA ACTCCACGTA CAATGTGTCC GTTTCAACAC GGATGGTCAT GGTTGAGGAG 
TTTAAACAAG GTCTTGCTAT CACAGATGAA ATTTTGCTGA GTAAGGCAGA GTGGTCCAAA 
CTTTTTGAAG CTCCAAACTT CTTTCAAAAG TACAAGCATT ATATTGTACT TCTAGCAAGT 
GCACCAACAG AAAAACAACG CCTGGAATGG GTGGGCTTGG TGGAATCAAA AATCCGAATC 
CTGGTTGGAA GCTTGGAGAA GAATGAATTT ATTACACTGG CTCATGTGAA TCCCCAGTCA 
TTTCCAGCAC CCAAAGAAAA TCCCGACAAG GAAGAATTTC GCACGATGTG GGTGATTGGG 
TTAGTGTTTA AAAAAACAGA AAACTCTGAA AACCTCAGTG TTGATCTCAC CTATGATATT 
CAGTCTTTCA CAGATACAGT TTATAGGCAA GCAATAAACA GCAAGATGTT TGAGGTGGAT 
ATGAAAATTG CTGCAATGCA TGTAAAAAGA AAGCAACTCC ATCAACTACT ACCTAATCAT 
GTGCTTCAGA AAAAGAAAAA GCATTCAACA GAAGGTGTCA AATTGACAGC TCTCAATGAC 
AGCAGCCTCG ACTTGTCTAT GGACAGTGAT AACAGCATGT CTGTGCCTTC ACCTACTAGT 
GCTACGAAGA CCAGTCCATT GAACAGTTCT GGCAGCTCTC AGGGAAACAG TCCTGCTCCA 
GCTGTAACAG CAGCATCTGT GACCAACATA CAGGCTACTG AAGTTTCTGT GCCACAAGTA 
AATTCCAGTG AAAGCTCAGG GGGTACATCG AGTGAAAGCA TTCCTCAAAC TGCCACACAA 
CCAGCCATTT CTCCACCACC AAAGCCTACG GTCTCCAGAG TTGTTTCTTC AACACGTCTG 
GTAAACCCAC CACCTAGATC TTCAGGAAAT GCAGCAACTT CAGGAAATGC AGCAACAAAA 
ATACCTACTC CTATAGTAGG AGTCAAGAGG ACATCCTCAC CTCATAAAGA AGAGAGTCCC 
AAGAAAACCA AAACAGAAGA GAAAACATCC AGTACAGACC TTTCTGATAT CCCTGCTCTC 
CCTGCAAATC CTATTCCTGT TATCAAGAAT TCAATAAAAC TGAGATTGAA TCGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001350595.1
CDS	212..2308
Translation	MPFPVTTQGSQQTQPPQKHYGITSPISLAAPKETDCVLTQKLIETLKPFGVFEEEEELQRRILILGKLNNLVKEWIREISESKNLPQSVIENVGGKIFTFGSYRLGVHTKGADIDALCVAPRHVDRSDFFTSFYDKLKLQEEVKDLRAVEEAFVPVIKLCFDGIEIDILFARLALQTIPEDLDLRDDSLLKNLDIRCIRSLNGCRVTDEILHLVPNIDNFRLTLRAIKLWAKRHNIYSNILGFLGGVSWAMLVARTCQLYPNAIASTLVHKFFLVFSKWEWPNPVLLKQPEECNLNLPVWDPRVNPSDRYHLMPIITPAYPQQNSTYNVSVSTRMVMVEEFKQGLAITDEILLSKAEWSKLFEAPNFFQKYKHYIVLLASAPTEKQRLEWVGLVESKIRILVGSLEKNEFITLAHVNPQSFPAPKENPDKEEFRTMWVIGLVFKKTENSENLSVDLTYDIQSFTDTVYRQAINSKMFEVDMKIAAMHVKRKQLHQLLPNHVLQKKKKHSTEGVKLTALNDSSLDLSMDSDNSMSVPSPTSATKTSPLNSSGSSQGNSPAPAVTAASVTNIQATEVSVPQVNSSESSGGTSSESIPQTATQPAISPPPKPTVSRVVSSTRLVNPPPRSSGNAATSGNAATKIPTPIVGVKRTSSPHKEESPKKTKTEEKTSSTDLSDIPALPANPIPVIKNSIKLRLNR

Target ORF information:

RefSeq Version	NM_001363666.2
Organism	Homo sapiens(human)
Definition	Homo sapiens poly(A) polymerase alpha (PAPOLA), transcript variant 10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001363666.2

ORF Insert Sequence:

ATGCCGTTTC CAGTTACAAC ACAGGGATCA CAACAAACAC AACCGCCACA GAAGCACTAT 
GGCATTACTT CTCCTATCAG CTTAGCAGCC CCCAAGGAGA CTGACTGCGT ACTTACACAG 
AAACTAATTG AGACATTGAA ACCCTTTGGG GTTTTTGAAG AGGAAGAGGA ACTGCAGCGC 
AGGATTTTAA TTTTGGGAAA ACTAAATAAC CTGGTAAAAG AGTGGATACG AGAAATCAGT 
GAAAGCAAGA ATCTTCCACA ATCTGTAATT GAAAATGTTG GAGGAAAAAT TTTTACATTT 
GGATCTTACA GATTAGGAGT GCATACAAAA GGTGCTGATA TTGATGCGTT GTGTGTTGCA 
CCAAGACATG TTGATCGAAG TGACTTTTTC ACCTCATTCT ATGATAAGTT GAAATTACAG 
GAAGAAGTAA AAGATTTAAG AGCTGTTGAA GAGGCATTCG TACCAGTTAT TAAACTCTGT 
TTTGATGGGA TAGAGATTGA TATTTTGTTT GCAAGATTAG CACTGCAGAC AATTCCTGAA 
GATTTGGATC TACGAGATGA CAGTCTGCTA AAAAATTTAG ATATAAGATG TATAAGAAGT 
CTTAACGGTT GCAGGGTAAC CGATGAAATT TTACATCTAG TACCAAACAT TGACAACTTC 
AGGTTAACTC TGAGAGCTAT CAAACTATGG GCCAAACGCC ACAACATCTA TTCCAATATA 
TTAGGTTTCC TCGGTGGTGT TTCCTGGGCT ATGCTAGTAG CAAGAACTTG CCAGCTTTAT 
CCAAATGCAA TAGCATCAAC TCTTGTACAT AAATTTTTCT TGGTATTTTC TAAATGGGAA 
TGGCCAAATC CAGTGCTATT GAAACAGCCT GAAGAATGCA ATCTTAATTT GCCTGTATGG 
GACCCAAGGG TAAACCCCAG TGATAGGTAC CATCTTATGC CTATAATTAC ACCAGCATAC 
CCACAACAGA ACTCCACGTA CAATGTGTCC GTTTCAACAC GGATGGTCAT GGTTGAGGAG 
TTTAAACAAG GTCTTGCTAT CACAGATGAA ATTTTGCTGA GTAAGGCAGA GTGGTCCAAA 
CTTTTTGAAG CTCCAAACTT CTTTCAAAAG TACAAGCATT ATATTGTACT TCTAGCAAGT 
GCACCAACAG AAAAACAACG CCTGGAATGG GTGGGCTTGG TGGAATCAAA AATCCGAATC 
CTGGTTGGAA GCTTGGAGAA GAATGAATTT ATTACACTGG CTCATGTGAA TCCCCAGTCA 
TTTCCAGCAC CCAAAGAAAA TCCCGACAAG GAAGAATTTC GCACGATGTG GGTGATTGGG 
TTAGTGTTTA AAAAAACAGA AAACTCTGAA AACCTCAGTG TTGATCTCAC CTATGATATT 
CAGTCTTTCA CAGATACAGT TTATAGGCAA GCAATAAACA GCAAGATGTT TGAGGTGGAT 
ATGAAAATTG CTGCAATGCA TGTAAAAAGA AAGCAACTCC ATCAACTACT ACCTAATCAT 
GTGCTTCAGA AAAAGAAAAA GCATTCAACA GAAGGTGTCA AATTGACAGC TCTCAATGAC 
AGCAGCCTCG ACTTGTCTAT GGACAGTGAT AACAGCATGT CTGTGCCTTC ACCTACTAGT 
GCTACGAAGA CCAGTCCATT GAACAGTTCT GGCAGCTCTC AGGGAAACAG TCCTGCTCCA 
GCTGTAACAG CAGCATCTGT GACCAACATA CAGGCTACTG AAGTTTCTGT GCCACAAGTA 
AATTCCAGTG AAAGCTCAGG GGGTACATCG AGTGAAAGCA TTCCTCAAAC TGCCACACAA 
CCAGCCATTT CTCCACCACC AAAGCCTACG GTCTCCAGAG TTGTTTCTTC AACACGTCTG 
GTAAACCCAC CACCTAGATC TTCAGGAAAT GCAGCAACTT CAGGAAATGC AGCAACAAAA 
ATACCTACTC CTATAGTAGG AGTCAAGAGG ACATCCTCAC CTCATAAAGA AGAGAGTCCC 
AAGAAAACCA AAACAGAAGA GAAAACATCC AGTACAGACC TTTCTGATAT CCCTGCTCTC 
CCTGCAAATC CTATTCCTGT TATCAAGAAT TCAATAAAAC TGAGATTGAA TCGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PAPOLA ( XM_017020935.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016876424.1 Homo sapiens poly(A) polymerase alpha (PAPOLA), transcript variant X2, mRNA.

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CloneID	OHu84741
Clone ID Related Accession (Same CDS sequence)	NM_001363664.1 , XM_017020935.1 , NM_001363664.2
Accession Version	XM_017020935.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2172bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	poly(A) polymerase alpha isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGTTTC CAGTTACAAC ACAGGGATCA CAACAAACAC AACCGCCACA GAAGCACTAT 
GGCATTACTT CTCCTATCAG CTTAGCAGCC CCCAAGGAGA CTGACTGCGT ACTTACACAG 
AAACTAATTG AGACATTGAA ACCCTTTGGG GTTTTTGAAG AGGAAGAGGA ACTGCAGCGC 
AGGATTTTAA TTTTGGGAAA ACTAAATAAC CTGGTAAAAG AGTGGATACG AGAAATCAGT 
GAAAGCAAGA ATCTTCCACA ATCTGTAATT GAAAATGTTG GAGGAAAAAT TTTTACATTT 
GGATCTTACA GATTAGGAGT GCATACAAAA GGTGCTGATA TTGATGCGTT GTGTGTTGCA 
CCAAGACATG TTGATCGAAG TGACTTTTTC ACCTCATTCT ATGATAAGTT GAAATTACAG 
GAAGAAGTAA AAGATTTAAG AGCTGTTGAA GAGGCATTCG TACCAGTTAT TAAACTCTGT 
TTTGATGGGA TAGAGATTGA TATTTTGTTT GCAAGATTAG CACTGCAGAC AATTCCTGAA 
GATTTGGATC TACGAGATGA CAGTCTGCTA AAAAATTTAG ATATAAGATG TATAAGAAGT 
CTTAACGGTT GCAGGGTAAC CGATGAAATT TTACATCTAG TACCAAACAT TGACAACTTC 
AGGTTAACTC TGAGAGCTAT CAAACTATGG GCCAAACGCC ACAACATCTA TTCCAATATA 
TTAGGTTTCC TCGGTGGTGT TTCCTGGGCT ATGCTAGTAG CAAGAACTTG CCAGCTTTAT 
CCAAATGCAA TAGCATCAAC TCTTGTACAT AAATTTTTCT TGGTATTTTC TAAATGGGAA 
TGGCCAAATC CAGTGCTATT GAAACAGCCT GAAGAATGCA ATCTTAATTT GCCTGTATGG 
GACCCAAGGG TAAACCCCAG TGATAGGTAC CATCTTATGC CTATAATTAC ACCAGCATAC 
CCACAACAGA ACTCCACGTA CAATGTGTCC GTTTCAACAC GGATGGTCAT GGTTGAGGAG 
TTTAAACAAG GTCTTGCTAT CACAGATGAA ATTTTGCTGA GTAAGGCAGA GTGGTCCAAA 
CTTTTTGAAG CTCCAAACTT CTTTCAAAAG TACAAGCATT ATATTGTACT TCTAGCAAGT 
GCACCAACAG AAAAACAACG CCTGGAATGG GTGGGCTTGG TGGAATCAAA AATCCGAATC 
CTGGTTGGAA GCTTGGAGAA GAATGAATTT ATTACACTGG CTCATGTGAA TCCCCAGTCA 
TTTCCAGCAC CCAAAGAAAA TCCCGACAAG GAAGAATTTC GCACGATGTG GGTGATTGGG 
TTAGTGTTTA AAAAAACAGA AAACTCTGAA AACCTCAGTG TTGATCTCAC CTATGATATT 
CAGTCTTTCA CAGATACAGT TTATAGGCAA GCAATAAACA GCAAGATGTT TGAGGTGGAT 
ATGAAAATTG CTGCAATGCA TGTAAAAAGA AAGCAACTCC ATCAACTACT ACCTAATCAT 
GTGCTTCAGA AAAAGAAAAA GCATTCAACA GAAGGTGTCA AATTGACAGC TCTCAATGAC 
AGCAGCCTCG ACTTGTCTAT GGACAGTGAT AACAGCATGT CTGTGCCTTC ACCTACTAGT 
GCTACGAAGA CCAGTCCATT GAACAGTTCT GGCAGCTCTC AGGGAAACAG TCCTGCTCCA 
GCTGTAACAG CAGCATCTGT GACCAACATA CAGGCTACTG AAGTTTCTGT GCCACAAGTA 
AATTCCAGTG AAAGCTCAGG GGGTACATCG AGTGAAAGCA TTCCTCAAAC TGCCACACAA 
CCAGCCATTT CTCCACCACC AAAGCCTACG GTCTCCAGAG TTGTTTCTTC AACACGTCTG 
GTAAACCCAC CACCTAGATC TTCAGGAAAT GCAGCAACTT CAGGAAATGC AGCAACAAAA 
ATACCTACTC CTATAGTAGG AGTCAAGAGG ACATCCTCAC CTCATAAAGA AGAGAGTCCC 
AAGAAAACCA AAACAGAAGA GGAACAACTT GATACAGAGA CAAGTACAAC TCAATCAGAA 
ACTATTCAGA CAGCGGCTTC TCTGTTGGCC TCTCAGAAAA CATCCAGTAC AGACCTTTCT 
GATATCCCTG CTCTCCCTGC AAATCCTATT CCTGTTATCA AGAATTCAAT AAAACTGAGA 
TTGAATCGGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016876424.1
CDS	214..2385
Translation	MPFPVTTQGSQQTQPPQKHYGITSPISLAAPKETDCVLTQKLIETLKPFGVFEEEEELQRRILILGKLNNLVKEWIREISESKNLPQSVIENVGGKIFTFGSYRLGVHTKGADIDALCVAPRHVDRSDFFTSFYDKLKLQEEVKDLRAVEEAFVPVIKLCFDGIEIDILFARLALQTIPEDLDLRDDSLLKNLDIRCIRSLNGCRVTDEILHLVPNIDNFRLTLRAIKLWAKRHNIYSNILGFLGGVSWAMLVARTCQLYPNAIASTLVHKFFLVFSKWEWPNPVLLKQPEECNLNLPVWDPRVNPSDRYHLMPIITPAYPQQNSTYNVSVSTRMVMVEEFKQGLAITDEILLSKAEWSKLFEAPNFFQKYKHYIVLLASAPTEKQRLEWVGLVESKIRILVGSLEKNEFITLAHVNPQSFPAPKENPDKEEFRTMWVIGLVFKKTENSENLSVDLTYDIQSFTDTVYRQAINSKMFEVDMKIAAMHVKRKQLHQLLPNHVLQKKKKHSTEGVKLTALNDSSLDLSMDSDNSMSVPSPTSATKTSPLNSSGSSQGNSPAPAVTAASVTNIQATEVSVPQVNSSESSGGTSSESIPQTATQPAISPPPKPTVSRVVSSTRLVNPPPRSSGNAATSGNAATKIPTPIVGVKRTSSPHKEESPKKTKTEEEQLDTETSTTQSETIQTAASLLASQKTSSTDLSDIPALPANPIPVIKNSIKLRLNR

Target ORF information:

RefSeq Version	XM_017020935.1
Organism	Homo sapiens(human)
Definition	Homo sapiens poly(A) polymerase alpha (PAPOLA), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017020935.1

ORF Insert Sequence:

ATGCCGTTTC CAGTTACAAC ACAGGGATCA CAACAAACAC AACCGCCACA GAAGCACTAT 
GGCATTACTT CTCCTATCAG CTTAGCAGCC CCCAAGGAGA CTGACTGCGT ACTTACACAG 
AAACTAATTG AGACATTGAA ACCCTTTGGG GTTTTTGAAG AGGAAGAGGA ACTGCAGCGC 
AGGATTTTAA TTTTGGGAAA ACTAAATAAC CTGGTAAAAG AGTGGATACG AGAAATCAGT 
GAAAGCAAGA ATCTTCCACA ATCTGTAATT GAAAATGTTG GAGGAAAAAT TTTTACATTT 
GGATCTTACA GATTAGGAGT GCATACAAAA GGTGCTGATA TTGATGCGTT GTGTGTTGCA 
CCAAGACATG TTGATCGAAG TGACTTTTTC ACCTCATTCT ATGATAAGTT GAAATTACAG 
GAAGAAGTAA AAGATTTAAG AGCTGTTGAA GAGGCATTCG TACCAGTTAT TAAACTCTGT 
TTTGATGGGA TAGAGATTGA TATTTTGTTT GCAAGATTAG CACTGCAGAC AATTCCTGAA 
GATTTGGATC TACGAGATGA CAGTCTGCTA AAAAATTTAG ATATAAGATG TATAAGAAGT 
CTTAACGGTT GCAGGGTAAC CGATGAAATT TTACATCTAG TACCAAACAT TGACAACTTC 
AGGTTAACTC TGAGAGCTAT CAAACTATGG GCCAAACGCC ACAACATCTA TTCCAATATA 
TTAGGTTTCC TCGGTGGTGT TTCCTGGGCT ATGCTAGTAG CAAGAACTTG CCAGCTTTAT 
CCAAATGCAA TAGCATCAAC TCTTGTACAT AAATTTTTCT TGGTATTTTC TAAATGGGAA 
TGGCCAAATC CAGTGCTATT GAAACAGCCT GAAGAATGCA ATCTTAATTT GCCTGTATGG 
GACCCAAGGG TAAACCCCAG TGATAGGTAC CATCTTATGC CTATAATTAC ACCAGCATAC 
CCACAACAGA ACTCCACGTA CAATGTGTCC GTTTCAACAC GGATGGTCAT GGTTGAGGAG 
TTTAAACAAG GTCTTGCTAT CACAGATGAA ATTTTGCTGA GTAAGGCAGA GTGGTCCAAA 
CTTTTTGAAG CTCCAAACTT CTTTCAAAAG TACAAGCATT ATATTGTACT TCTAGCAAGT 
GCACCAACAG AAAAACAACG CCTGGAATGG GTGGGCTTGG TGGAATCAAA AATCCGAATC 
CTGGTTGGAA GCTTGGAGAA GAATGAATTT ATTACACTGG CTCATGTGAA TCCCCAGTCA 
TTTCCAGCAC CCAAAGAAAA TCCCGACAAG GAAGAATTTC GCACGATGTG GGTGATTGGG 
TTAGTGTTTA AAAAAACAGA AAACTCTGAA AACCTCAGTG TTGATCTCAC CTATGATATT 
CAGTCTTTCA CAGATACAGT TTATAGGCAA GCAATAAACA GCAAGATGTT TGAGGTGGAT 
ATGAAAATTG CTGCAATGCA TGTAAAAAGA AAGCAACTCC ATCAACTACT ACCTAATCAT 
GTGCTTCAGA AAAAGAAAAA GCATTCAACA GAAGGTGTCA AATTGACAGC TCTCAATGAC 
AGCAGCCTCG ACTTGTCTAT GGACAGTGAT AACAGCATGT CTGTGCCTTC ACCTACTAGT 
GCTACGAAGA CCAGTCCATT GAACAGTTCT GGCAGCTCTC AGGGAAACAG TCCTGCTCCA 
GCTGTAACAG CAGCATCTGT GACCAACATA CAGGCTACTG AAGTTTCTGT GCCACAAGTA 
AATTCCAGTG AAAGCTCAGG GGGTACATCG AGTGAAAGCA TTCCTCAAAC TGCCACACAA 
CCAGCCATTT CTCCACCACC AAAGCCTACG GTCTCCAGAG TTGTTTCTTC AACACGTCTG 
GTAAACCCAC CACCTAGATC TTCAGGAAAT GCAGCAACTT CAGGAAATGC AGCAACAAAA 
ATACCTACTC CTATAGTAGG AGTCAAGAGG ACATCCTCAC CTCATAAAGA AGAGAGTCCC 
AAGAAAACCA AAACAGAAGA GGAACAACTT GATACAGAGA CAAGTACAAC TCAATCAGAA 
ACTATTCAGA CAGCGGCTTC TCTGTTGGCC TCTCAGAAAA CATCCAGTAC AGACCTTTCT 
GATATCCCTG CTCTCCCTGC AAATCCTATT CCTGTTATCA AGAATTCAAT AAAACTGAGA 
TTGAATCGGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PAPOLA ( XM_017020936.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016876425.1 Homo sapiens poly(A) polymerase alpha (PAPOLA), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHu84742
Clone ID Related Accession (Same CDS sequence)	NM_001363666.1 , XM_017020936.1 , NM_001363666.2
Accession Version	XM_017020936.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2097bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	poly(A) polymerase alpha isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGTTTC CAGTTACAAC ACAGGGATCA CAACAAACAC AACCGCCACA GAAGCACTAT 
GGCATTACTT CTCCTATCAG CTTAGCAGCC CCCAAGGAGA CTGACTGCGT ACTTACACAG 
AAACTAATTG AGACATTGAA ACCCTTTGGG GTTTTTGAAG AGGAAGAGGA ACTGCAGCGC 
AGGATTTTAA TTTTGGGAAA ACTAAATAAC CTGGTAAAAG AGTGGATACG AGAAATCAGT 
GAAAGCAAGA ATCTTCCACA ATCTGTAATT GAAAATGTTG GAGGAAAAAT TTTTACATTT 
GGATCTTACA GATTAGGAGT GCATACAAAA GGTGCTGATA TTGATGCGTT GTGTGTTGCA 
CCAAGACATG TTGATCGAAG TGACTTTTTC ACCTCATTCT ATGATAAGTT GAAATTACAG 
GAAGAAGTAA AAGATTTAAG AGCTGTTGAA GAGGCATTCG TACCAGTTAT TAAACTCTGT 
TTTGATGGGA TAGAGATTGA TATTTTGTTT GCAAGATTAG CACTGCAGAC AATTCCTGAA 
GATTTGGATC TACGAGATGA CAGTCTGCTA AAAAATTTAG ATATAAGATG TATAAGAAGT 
CTTAACGGTT GCAGGGTAAC CGATGAAATT TTACATCTAG TACCAAACAT TGACAACTTC 
AGGTTAACTC TGAGAGCTAT CAAACTATGG GCCAAACGCC ACAACATCTA TTCCAATATA 
TTAGGTTTCC TCGGTGGTGT TTCCTGGGCT ATGCTAGTAG CAAGAACTTG CCAGCTTTAT 
CCAAATGCAA TAGCATCAAC TCTTGTACAT AAATTTTTCT TGGTATTTTC TAAATGGGAA 
TGGCCAAATC CAGTGCTATT GAAACAGCCT GAAGAATGCA ATCTTAATTT GCCTGTATGG 
GACCCAAGGG TAAACCCCAG TGATAGGTAC CATCTTATGC CTATAATTAC ACCAGCATAC 
CCACAACAGA ACTCCACGTA CAATGTGTCC GTTTCAACAC GGATGGTCAT GGTTGAGGAG 
TTTAAACAAG GTCTTGCTAT CACAGATGAA ATTTTGCTGA GTAAGGCAGA GTGGTCCAAA 
CTTTTTGAAG CTCCAAACTT CTTTCAAAAG TACAAGCATT ATATTGTACT TCTAGCAAGT 
GCACCAACAG AAAAACAACG CCTGGAATGG GTGGGCTTGG TGGAATCAAA AATCCGAATC 
CTGGTTGGAA GCTTGGAGAA GAATGAATTT ATTACACTGG CTCATGTGAA TCCCCAGTCA 
TTTCCAGCAC CCAAAGAAAA TCCCGACAAG GAAGAATTTC GCACGATGTG GGTGATTGGG 
TTAGTGTTTA AAAAAACAGA AAACTCTGAA AACCTCAGTG TTGATCTCAC CTATGATATT 
CAGTCTTTCA CAGATACAGT TTATAGGCAA GCAATAAACA GCAAGATGTT TGAGGTGGAT 
ATGAAAATTG CTGCAATGCA TGTAAAAAGA AAGCAACTCC ATCAACTACT ACCTAATCAT 
GTGCTTCAGA AAAAGAAAAA GCATTCAACA GAAGGTGTCA AATTGACAGC TCTCAATGAC 
AGCAGCCTCG ACTTGTCTAT GGACAGTGAT AACAGCATGT CTGTGCCTTC ACCTACTAGT 
GCTACGAAGA CCAGTCCATT GAACAGTTCT GGCAGCTCTC AGGGAAACAG TCCTGCTCCA 
GCTGTAACAG CAGCATCTGT GACCAACATA CAGGCTACTG AAGTTTCTGT GCCACAAGTA 
AATTCCAGTG AAAGCTCAGG GGGTACATCG AGTGAAAGCA TTCCTCAAAC TGCCACACAA 
CCAGCCATTT CTCCACCACC AAAGCCTACG GTCTCCAGAG TTGTTTCTTC AACACGTCTG 
GTAAACCCAC CACCTAGATC TTCAGGAAAT GCAGCAACTT CAGGAAATGC AGCAACAAAA 
ATACCTACTC CTATAGTAGG AGTCAAGAGG ACATCCTCAC CTCATAAAGA AGAGAGTCCC 
AAGAAAACCA AAACAGAAGA GAAAACATCC AGTACAGACC TTTCTGATAT CCCTGCTCTC 
CCTGCAAATC CTATTCCTGT TATCAAGAAT TCAATAAAAC TGAGATTGAA TCGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016876425.1
CDS	214..2310
Translation	MPFPVTTQGSQQTQPPQKHYGITSPISLAAPKETDCVLTQKLIETLKPFGVFEEEEELQRRILILGKLNNLVKEWIREISESKNLPQSVIENVGGKIFTFGSYRLGVHTKGADIDALCVAPRHVDRSDFFTSFYDKLKLQEEVKDLRAVEEAFVPVIKLCFDGIEIDILFARLALQTIPEDLDLRDDSLLKNLDIRCIRSLNGCRVTDEILHLVPNIDNFRLTLRAIKLWAKRHNIYSNILGFLGGVSWAMLVARTCQLYPNAIASTLVHKFFLVFSKWEWPNPVLLKQPEECNLNLPVWDPRVNPSDRYHLMPIITPAYPQQNSTYNVSVSTRMVMVEEFKQGLAITDEILLSKAEWSKLFEAPNFFQKYKHYIVLLASAPTEKQRLEWVGLVESKIRILVGSLEKNEFITLAHVNPQSFPAPKENPDKEEFRTMWVIGLVFKKTENSENLSVDLTYDIQSFTDTVYRQAINSKMFEVDMKIAAMHVKRKQLHQLLPNHVLQKKKKHSTEGVKLTALNDSSLDLSMDSDNSMSVPSPTSATKTSPLNSSGSSQGNSPAPAVTAASVTNIQATEVSVPQVNSSESSGGTSSESIPQTATQPAISPPPKPTVSRVVSSTRLVNPPPRSSGNAATSGNAATKIPTPIVGVKRTSSPHKEESPKKTKTEEKTSSTDLSDIPALPANPIPVIKNSIKLRLNR

Target ORF information:

RefSeq Version	XM_017020936.1
Organism	Homo sapiens(human)
Definition	Homo sapiens poly(A) polymerase alpha (PAPOLA), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017020936.1

ORF Insert Sequence:

ATGCCGTTTC CAGTTACAAC ACAGGGATCA CAACAAACAC AACCGCCACA GAAGCACTAT 
GGCATTACTT CTCCTATCAG CTTAGCAGCC CCCAAGGAGA CTGACTGCGT ACTTACACAG 
AAACTAATTG AGACATTGAA ACCCTTTGGG GTTTTTGAAG AGGAAGAGGA ACTGCAGCGC 
AGGATTTTAA TTTTGGGAAA ACTAAATAAC CTGGTAAAAG AGTGGATACG AGAAATCAGT 
GAAAGCAAGA ATCTTCCACA ATCTGTAATT GAAAATGTTG GAGGAAAAAT TTTTACATTT 
GGATCTTACA GATTAGGAGT GCATACAAAA GGTGCTGATA TTGATGCGTT GTGTGTTGCA 
CCAAGACATG TTGATCGAAG TGACTTTTTC ACCTCATTCT ATGATAAGTT GAAATTACAG 
GAAGAAGTAA AAGATTTAAG AGCTGTTGAA GAGGCATTCG TACCAGTTAT TAAACTCTGT 
TTTGATGGGA TAGAGATTGA TATTTTGTTT GCAAGATTAG CACTGCAGAC AATTCCTGAA 
GATTTGGATC TACGAGATGA CAGTCTGCTA AAAAATTTAG ATATAAGATG TATAAGAAGT 
CTTAACGGTT GCAGGGTAAC CGATGAAATT TTACATCTAG TACCAAACAT TGACAACTTC 
AGGTTAACTC TGAGAGCTAT CAAACTATGG GCCAAACGCC ACAACATCTA TTCCAATATA 
TTAGGTTTCC TCGGTGGTGT TTCCTGGGCT ATGCTAGTAG CAAGAACTTG CCAGCTTTAT 
CCAAATGCAA TAGCATCAAC TCTTGTACAT AAATTTTTCT TGGTATTTTC TAAATGGGAA 
TGGCCAAATC CAGTGCTATT GAAACAGCCT GAAGAATGCA ATCTTAATTT GCCTGTATGG 
GACCCAAGGG TAAACCCCAG TGATAGGTAC CATCTTATGC CTATAATTAC ACCAGCATAC 
CCACAACAGA ACTCCACGTA CAATGTGTCC GTTTCAACAC GGATGGTCAT GGTTGAGGAG 
TTTAAACAAG GTCTTGCTAT CACAGATGAA ATTTTGCTGA GTAAGGCAGA GTGGTCCAAA 
CTTTTTGAAG CTCCAAACTT CTTTCAAAAG TACAAGCATT ATATTGTACT TCTAGCAAGT 
GCACCAACAG AAAAACAACG CCTGGAATGG GTGGGCTTGG TGGAATCAAA AATCCGAATC 
CTGGTTGGAA GCTTGGAGAA GAATGAATTT ATTACACTGG CTCATGTGAA TCCCCAGTCA 
TTTCCAGCAC CCAAAGAAAA TCCCGACAAG GAAGAATTTC GCACGATGTG GGTGATTGGG 
TTAGTGTTTA AAAAAACAGA AAACTCTGAA AACCTCAGTG TTGATCTCAC CTATGATATT 
CAGTCTTTCA CAGATACAGT TTATAGGCAA GCAATAAACA GCAAGATGTT TGAGGTGGAT 
ATGAAAATTG CTGCAATGCA TGTAAAAAGA AAGCAACTCC ATCAACTACT ACCTAATCAT 
GTGCTTCAGA AAAAGAAAAA GCATTCAACA GAAGGTGTCA AATTGACAGC TCTCAATGAC 
AGCAGCCTCG ACTTGTCTAT GGACAGTGAT AACAGCATGT CTGTGCCTTC ACCTACTAGT 
GCTACGAAGA CCAGTCCATT GAACAGTTCT GGCAGCTCTC AGGGAAACAG TCCTGCTCCA 
GCTGTAACAG CAGCATCTGT GACCAACATA CAGGCTACTG AAGTTTCTGT GCCACAAGTA 
AATTCCAGTG AAAGCTCAGG GGGTACATCG AGTGAAAGCA TTCCTCAAAC TGCCACACAA 
CCAGCCATTT CTCCACCACC AAAGCCTACG GTCTCCAGAG TTGTTTCTTC AACACGTCTG 
GTAAACCCAC CACCTAGATC TTCAGGAAAT GCAGCAACTT CAGGAAATGC AGCAACAAAA 
ATACCTACTC CTATAGTAGG AGTCAAGAGG ACATCCTCAC CTCATAAAGA AGAGAGTCCC 
AAGAAAACCA AAACAGAAGA GAAAACATCC AGTACAGACC TTTCTGATAT CCCTGCTCTC 
CCTGCAAATC CTATTCCTGT TATCAAGAAT TCAATAAAAC TGAGATTGAA TCGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	PAPOLA
Entrez Gene ID	10914
Full Name	poly(A) polymerase alpha
Synonyms	PAP
General protein information	Preferred Names poly(A) polymerase alpha Names poly(A) polymerase alpha PAP-alpha polynucleotide adenylyltransferase alpha
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 14 Map Location: 14q32.2
Summary	The protein encoded by this gene belongs to the poly(A) polymerase family. It is required for the addition of adenosine residues for the creation of the 3'-poly(A) tail of mRNAs. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011].
Disorder MIM:	605553

Bos taurus (cattle)	PAPOLA	NP_788820.1
Rattus norvegicus (Norway rat)	Papola	NP_001101526.1
Gallus gallus (chicken)	PAPOLA	NP_001185530.1
Oryza sativa (rice)	Os06g0558700	NP_001174846.1
	PAPOLA	XP_002805246.1
Mus musculus (house mouse)	Papola	NP_035242.1
Caenorhabditis elegans (roundworm)	pap-1	NP_505683.1
Caenorhabditis elegans (roundworm)	T15H9.6	NP_496067.1
Danio rerio (zebrafish)	papola	NP_991321.2
Caenorhabditis elegans (roundworm)	CELE_F43G6.5	NP_496524.1
Arabidopsis thaliana (thale cress)	PAPS2	NP_001031417.1
Oryza sativa (rice)	Os02g0227400	NP_001046352.2
Xenopus tropicalis (tropical clawed frog)	papola	NP_001136382.1
Homo sapiens (human)	PAPOLA	NP_116021.2
Pan troglodytes (chimpanzee)	PAPOLA	XP_003314562.1
Canis lupus familiaris (dog)	PAPOLA	XP_005623838.1
Arabidopsis thaliana (thale cress)	nPAP	NP_974668.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

PAPOLA cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

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