HCST cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	HCST
Entrez Gene ID	10870
Full Name	hematopoietic cell signal transducer
Synonyms	DAP10,KAP10,PIK3AP
General protein information	Preferred Names hematopoietic cell signal transducer Names hematopoietic cell signal transducer DNAX-activation protein 10 kinase assoc pro of ~10kDa kinase assoc protein membrane protein DAP10 phosphoinositide-3-kinase adaptor protein transmembrane adapter protein KAP10
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 19 Map Location: 19q13.12
Summary	This gene encodes a transmembrane signaling adaptor that contains a YxxM motif in its cytoplasmic domain. The encoded protein may form part of the immune recognition receptor complex with the C-type lectin-like receptor NKG2D. As part of this receptor complex, this protein may activate phosphatidylinositol 3-kinase dependent signaling pathways through its intracytoplasmic YxxM motif. This receptor complex may have a role in cell survival and proliferation by activation of NK and T cell responses. Alternative splicing results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008].
Disorder MIM:	604089

mRNA and Protein(s)

mRNA	Protein	Name
NM_014266.4	NP_055081.1	hematopoietic cell signal transducer isoform 1 precursor
XM_017026193.1	XP_016881682.1	hematopoietic cell signal transducer isoform X1
NM_001007469.1	NP_001007470.1	hematopoietic cell signal transducer isoform 2 precursor
NM_014266.3	NP_055081.1	hematopoietic cell signal transducer isoform 1 precursor
NM_001007469.2	NP_001007470.1	hematopoietic cell signal transducer isoform 2 precursor

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Hcst	NP_001005900.1
Homo sapiens (human)	HCST	NP_055081.1
Pan troglodytes (chimpanzee)	HCST	XP_001159643.2
	HCST	NP_001028007.1
Bos taurus (cattle)	HCST	NP_001070445.1
Mus musculus (house mouse)	Hcst	NP_035957.2

Gene Ontology
Bibliography

Related articles in PubMed

Ubiquitin-dependent endocytosis of NKG2D-DAP10 receptor complexes activates signaling and functions in human NK cells.
Quatrini L, Molfetta R, Zitti B, Peruzzi G, Fionda C, Capuano C, Galandrini R, Cippitelli M, Santoni A, Paolini R
Science signaling8(400)ra108(2015 Oct)

Identification and Characterization of CD300H, a New Member of the Human CD300 Immunoreceptor Family.
Niizuma K, Tahara-Hanaoka S, Noguchi E, Shibuya A
The Journal of biological chemistry290(36)22298-308(2015 Sep)

DNAX-activating protein 10 (DAP10) membrane adaptor associates with receptor for advanced glycation end products (RAGE) and modulates the RAGE-triggered signaling pathway in human keratinocytes.
Sakaguchi M, Murata H, Aoyama Y, Hibino T, Putranto EW, Ruma IM, Inoue Y, Sakaguchi Y, Yamamoto K, Kinoshita R, Futami J, Kataoka K, Iwatsuki K, Huh NH
The Journal of biological chemistry289(34)23389-402(2014 Aug)

TGF-?1 down-regulation of NKG2D/DAP10 and 2B4/SAP expression on human NK cells contributes to HBV persistence.
Sun C, Fu B, Gao Y, Liao X, Sun R, Tian Z, Wei H
PLoS pathogens8(3)e1002594(2012)

Complex regulation of human NKG2D-DAP10 cell surface expression: opposing roles of the ?c cytokines and TGF-?1.
Park YP, Choi SC, Kiesler P, Gil-Krzewska A, Borrego F, Weck J, Krzewski K, Coligan JE
Blood118(11)3019-27(2011 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These results suggest that NKG2D-DAP10 endocytosis represents a means to decrease cell surface receptor abundance, as well as to control signaling outcome in cytotoxic lymphocytes.
Title: Ubiquitin-dependent endocytosis of NKG2D-DAP10 receptor complexes activates signaling and functions in human NK cells.

findings indicate that the functional interaction between RAGE and DAP10 coordinately regulates S100A8/A9-mediated survival and/or apoptotic response of keratinocytes
Title: DNAX-activating protein 10 (DAP10) membrane adaptor associates with receptor for advanced glycation end products (RAGE) and modulates the RAGE-triggered signaling pathway in human keratinocytes.

TGF-beta1 may reduce the expression of NKG2D/DAP10 and 2B4/SAPin patients with hepatitis B.
Title: TGF-?1 down-regulation of NKG2D/DAP10 and 2B4/SAP expression on human NK cells contributes to HBV persistence.

Other gamma(c) cytokines act similarly to IL-2 in up-regulating DAP10 expression and NKG2D-DAP10 surface expression.
Title: Complex regulation of human NKG2D-DAP10 cell surface expression: opposing roles of the ?c cytokines and TGF-?1.

TGF-BETA1 down-modulates surface NKG2D expression by controlling the transcriptional and translational levels of DAP10.
Title: A possible mechanism of impaired NK cytotoxicity in cancer patients: down-regulation of DAP10 by TGF-beta1.

The following HCST gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HCST cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu31189	NM_014266.4 Latest version!	Homo sapiens hematopoietic cell signal transducer (HCST), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu31181	NM_001007469.2 Latest version!	Homo sapiens hematopoietic cell signal transducer (HCST), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OHu88137	XM_017026193.1 Latest version!	Homo sapiens hematopoietic cell signal transducer (HCST), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
View Old Accession Versions >>
OHu31189	NM_014266.3	Homo sapiens hematopoietic cell signal transducer (HCST), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu31181	NM_001007469.1	Homo sapiens hematopoietic cell signal transducer (HCST), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HCST ( NM_014266.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_055081.1 Homo sapiens hematopoietic cell signal transducer (HCST), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu31189
Clone ID Related Accession (Same CDS sequence)	NM_014266.4 , NM_014266.3
Accession Version	NM_014266.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 282bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-25
Organism	Homo sapiens(human)
Product	hematopoietic cell signal transducer isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL050163.1 and AD000864.1. On May 17, 2019 this sequence version replaced NM_014266.3. Transcript Variant: This variant (1) encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BP226120.1, BC035931.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000246551.9/ ENSP00000246551.3 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGATCCATC TGGGTCACAT CCTCTTCCTG CTTTTGCTCC CAGTGGCTGC AGCTCAGACG 
ACTCCAGGAG AGAGATCATC ACTCCCTGCC TTTTACCCTG GCACTTCAGG CTCTTGTTCC 
GGATGTGGGT CCCTCTCTCT GCCGCTCCTG GCAGGCCTCG TGGCTGCTGA TGCGGTGGCA 
TCGCTGCTCA TCGTGGGGGC GGTGTTCCTG TGCGCACGCC CACGCCGCAG CCCCGCCCAA 
GAAGATGGCA AAGTCTACAT CAACATGCCA GGCAGGGGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055081.1
CDS	66..347
Translation	MIHLGHILFLLLLPVAAAQTTPGERSSLPAFYPGTSGSCSGCGSLSLPLLAGLVAADAVASLLIVGAVFLCARPRRSPAQEDGKVYINMPGRG

Target ORF information:

RefSeq Version	NM_014266.4
Organism	Homo sapiens(human)
Definition	Homo sapiens hematopoietic cell signal transducer (HCST), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014266.4

ORF Insert Sequence:

ATGATCCATC TGGGTCACAT CCTCTTCCTG CTTTTGCTCC CAGTGGCTGC AGCTCAGACG 
ACTCCAGGAG AGAGATCATC ACTCCCTGCC TTTTACCCTG GCACTTCAGG CTCTTGTTCC 
GGATGTGGGT CCCTCTCTCT GCCGCTCCTG GCAGGCCTCG TGGCTGCTGA TGCGGTGGCA 
TCGCTGCTCA TCGTGGGGGC GGTGTTCCTG TGCGCACGCC CACGCCGCAG CCCCGCCCAA 
GAAGATGGCA AAGTCTACAT CAACATGCCA GGCAGGGGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HCST ( NM_014266.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_055081.1 Homo sapiens hematopoietic cell signal transducer (HCST), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu31189
Clone ID Related Accession (Same CDS sequence)	NM_014266.4 , NM_014266.3
Accession Version	NM_014266.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 282bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-15
Organism	Homo sapiens(human)
Product	hematopoietic cell signal transducer isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL050163.1. On Aug 15, 2003 this sequence version replaced NM_014266.2. Transcript Variant: This variant (1) encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AL050163.1, BC046348.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATCCATC TGGGTCACAT CCTCTTCCTG CTTTTGCTCC CAGTGGCTGC AGCTCAGACG 
ACTCCAGGAG AGAGATCATC ACTCCCTGCC TTTTACCCTG GCACTTCAGG CTCTTGTTCC 
GGATGTGGGT CCCTCTCTCT GCCGCTCCTG GCAGGCCTCG TGGCTGCTGA TGCGGTGGCA 
TCGCTGCTCA TCGTGGGGGC GGTGTTCCTG TGCGCACGCC CACGCCGCAG CCCCGCCCAA 
GAAGATGGCA AAGTCTACAT CAACATGCCA GGCAGGGGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055081.1
CDS	115..396
Translation	MIHLGHILFLLLLPVAAAQTTPGERSSLPAFYPGTSGSCSGCGSLSLPLLAGLVAADAVASLLIVGAVFLCARPRRSPAQEDGKVYINMPGRG

Target ORF information:

RefSeq Version	NM_014266.3
Organism	Homo sapiens(human)
Definition	Homo sapiens hematopoietic cell signal transducer (HCST), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014266.3

ORF Insert Sequence:

ATGATCCATC TGGGTCACAT CCTCTTCCTG CTTTTGCTCC CAGTGGCTGC AGCTCAGACG 
ACTCCAGGAG AGAGATCATC ACTCCCTGCC TTTTACCCTG GCACTTCAGG CTCTTGTTCC 
GGATGTGGGT CCCTCTCTCT GCCGCTCCTG GCAGGCCTCG TGGCTGCTGA TGCGGTGGCA 
TCGCTGCTCA TCGTGGGGGC GGTGTTCCTG TGCGCACGCC CACGCCGCAG CCCCGCCCAA 
GAAGATGGCA AAGTCTACAT CAACATGCCA GGCAGGGGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HCST ( NM_001007469.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001007470.1 Homo sapiens hematopoietic cell signal transducer (HCST), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu31181
Clone ID Related Accession (Same CDS sequence)	NM_001007469.1 , NM_001007469.2
Accession Version	NM_001007469.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 279bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-16
Organism	Homo sapiens(human)
Product	hematopoietic cell signal transducer isoform 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL050163.1 and AF072844.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1, resulting in a shorter protein (isoform 2). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BP296917.1, HY059653.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATCCATC TGGGTCACAT CCTCTTCCTG CTTTTGCTCC CAGTGGCTGC AGCTCAGACG 
ACTCCAGGAG AGAGATCATC ACTCCCTGCC TTTTACCCTG GCACTTCAGG CTCTTGTTCC 
GGATGTGGGT CCCTCTCTCT GCCGCTCCTG GCAGGCCTCG TGGCTGCTGA TGCGGTGGCA 
TCGCTGCTCA TCGTGGGGGC GGTGTTCCTG TGCGCACGCC CACGCCGCAG CCCCGCCCAA 
GATGGCAAAG TCTACATCAA CATGCCAGGC AGGGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001007470.1
CDS	115..393
Translation	MIHLGHILFLLLLPVAAAQTTPGERSSLPAFYPGTSGSCSGCGSLSLPLLAGLVAADAVASLLIVGAVFLCARPRRSPAQDGKVYINMPGRG

Target ORF information:

RefSeq Version	NM_001007469.1
Organism	Homo sapiens(human)
Definition	Homo sapiens hematopoietic cell signal transducer (HCST), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001007469.1

ORF Insert Sequence:

ATGATCCATC TGGGTCACAT CCTCTTCCTG CTTTTGCTCC CAGTGGCTGC AGCTCAGACG 
ACTCCAGGAG AGAGATCATC ACTCCCTGCC TTTTACCCTG GCACTTCAGG CTCTTGTTCC 
GGATGTGGGT CCCTCTCTCT GCCGCTCCTG GCAGGCCTCG TGGCTGCTGA TGCGGTGGCA 
TCGCTGCTCA TCGTGGGGGC GGTGTTCCTG TGCGCACGCC CACGCCGCAG CCCCGCCCAA 
GATGGCAAAG TCTACATCAA CATGCCAGGC AGGGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HCST ( NM_001007469.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001007470.1 Homo sapiens hematopoietic cell signal transducer (HCST), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu31181
Clone ID Related Accession (Same CDS sequence)	NM_001007469.1 , NM_001007469.2
Accession Version	NM_001007469.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 279bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-27
Organism	Homo sapiens(human)
Product	hematopoietic cell signal transducer isoform 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL050163.1 and AF072844.1. On May 31, 2019 this sequence version replaced NM_001007469.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1, resulting in a shorter protein (isoform 2). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF072844.1, HY283346.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATCCATC TGGGTCACAT CCTCTTCCTG CTTTTGCTCC CAGTGGCTGC AGCTCAGACG 
ACTCCAGGAG AGAGATCATC ACTCCCTGCC TTTTACCCTG GCACTTCAGG CTCTTGTTCC 
GGATGTGGGT CCCTCTCTCT GCCGCTCCTG GCAGGCCTCG TGGCTGCTGA TGCGGTGGCA 
TCGCTGCTCA TCGTGGGGGC GGTGTTCCTG TGCGCACGCC CACGCCGCAG CCCCGCCCAA 
GATGGCAAAG TCTACATCAA CATGCCAGGC AGGGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001007470.1
CDS	66..344
Translation	MIHLGHILFLLLLPVAAAQTTPGERSSLPAFYPGTSGSCSGCGSLSLPLLAGLVAADAVASLLIVGAVFLCARPRRSPAQDGKVYINMPGRG

Target ORF information:

RefSeq Version	NM_001007469.2
Organism	Homo sapiens(human)
Definition	Homo sapiens hematopoietic cell signal transducer (HCST), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001007469.2

ORF Insert Sequence:

ATGATCCATC TGGGTCACAT CCTCTTCCTG CTTTTGCTCC CAGTGGCTGC AGCTCAGACG 
ACTCCAGGAG AGAGATCATC ACTCCCTGCC TTTTACCCTG GCACTTCAGG CTCTTGTTCC 
GGATGTGGGT CCCTCTCTCT GCCGCTCCTG GCAGGCCTCG TGGCTGCTGA TGCGGTGGCA 
TCGCTGCTCA TCGTGGGGGC GGTGTTCCTG TGCGCACGCC CACGCCGCAG CCCCGCCCAA 
GATGGCAAAG TCTACATCAA CATGCCAGGC AGGGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HCST ( XM_017026193.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016881682.1 Homo sapiens hematopoietic cell signal transducer (HCST), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu88137
Clone ID Related Accession (Same CDS sequence)	XM_017026193.1
Accession Version	XM_017026193.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 435bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	hematopoietic cell signal transducer isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCCATC TGGGTCACAT CCTCTTCCTG CTTTTGCTCC CAGTGGCTGC AGCTCAGACG 
ACTCCAGGAG AGAGATCATC ACTCCCTGCC TTTTACCCTG GCACTTCAGC GCAACTCCAA 
GGAACCTGGG ACCCGCCCCC TCGCAGGGGA CTTCCTCTCT GCCTGTGGCC AAAGCACAGC 
CCCAGGACGC AGAGCTTGAG TTGTCTCCCT GTTCCGGCCC CCACTCTCCA GGCTCTTGTT 
CCGGATGTGG GTCCCTCTCT CTGCCGCTCC TGGCAGGCCT CGTGGCTGCT GATGCGGTGG 
CATCGCTGCT CATCGTGGGG GCGGTGTTCC TGTGCGCACG CCCACGCCGC AGCCCCGCCC 
AAGAAGATGG CAAAGTCTAC ATCAACATGC CAGGCAGGGG CTGACCCTCC TGCAGCTTGG 
ACCTTTGACT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016881682.1
CDS	230..664
Translation	MIHLGHILFLLLLPVAAAQTTPGERSSLPAFYPGTSAQLQGTWDPPPRRGLPLCLWPKHSPRTQSLSCLPVPAPTLQALVPDVGPSLCRSWQASWLLMRWHRCSSWGRCSCAHAHAAAPPKKMAKSTSTCQAGADPPAAWTFDF

Target ORF information:

RefSeq Version	XM_017026193.1
Organism	Homo sapiens(human)
Definition	Homo sapiens hematopoietic cell signal transducer (HCST), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017026193.1

ORF Insert Sequence:

ATGATCCATC TGGGTCACAT CCTCTTCCTG CTTTTGCTCC CAGTGGCTGC AGCTCAGACG 
ACTCCAGGAG AGAGATCATC ACTCCCTGCC TTTTACCCTG GCACTTCAGC GCAACTCCAA 
GGAACCTGGG ACCCGCCCCC TCGCAGGGGA CTTCCTCTCT GCCTGTGGCC AAAGCACAGC 
CCCAGGACGC AGAGCTTGAG TTGTCTCCCT GTTCCGGCCC CCACTCTCCA GGCTCTTGTT 
CCGGATGTGG GTCCCTCTCT CTGCCGCTCC TGGCAGGCCT CGTGGCTGCT GATGCGGTGG 
CATCGCTGCT CATCGTGGGG GCGGTGTTCC TGTGCGCACG CCCACGCCGC AGCCCCGCCC 
AAGAAGATGG CAAAGTCTAC ATCAACATGC CAGGCAGGGG CTGACCCTCC TGCAGCTTGG 
ACCTTTGACT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Ubiquitin-dependent endocytosis of NKG2D-DAP10 receptor complexes activates signaling and functions in human NK cells.
Science signaling8(400)ra108(2015 Oct)
Quatrini L,Molfetta R,Zitti B,Peruzzi G,Fionda C,Capuano C,Galandrini R,Cippitelli M,Santoni A,Paolini R

Identification and Characterization of CD300H, a New Member of the Human CD300 Immunoreceptor Family.
The Journal of biological chemistry290(36)22298-308(2015 Sep)
Niizuma K,Tahara-Hanaoka S,Noguchi E,Shibuya A

DNAX-activating protein 10 (DAP10) membrane adaptor associates with receptor for advanced glycation end products (RAGE) and modulates the RAGE-triggered signaling pathway in human keratinocytes.
The Journal of biological chemistry289(34)23389-402(2014 Aug)
Sakaguchi M,Murata H,Aoyama Y,Hibino T,Putranto EW,Ruma IM,Inoue Y,Sakaguchi Y,Yamamoto K,Kinoshita R,Futami J,Kataoka K,Iwatsuki K,Huh NH

TGF-?1 down-regulation of NKG2D/DAP10 and 2B4/SAP expression on human NK cells contributes to HBV persistence.
PLoS pathogens8(3)e1002594(2012)
Sun C,Fu B,Gao Y,Liao X,Sun R,Tian Z,Wei H

Complex regulation of human NKG2D-DAP10 cell surface expression: opposing roles of the ?c cytokines and TGF-?1.
Blood118(11)3019-27(2011 Sep)
Park YP,Choi SC,Kiesler P,Gil-Krzewska A,Borrego F,Weck J,Krzewski K,Coligan JE

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HCST cDNA ORF clone, Homo sapiens(human)

Gene

Clones

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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