KDM5B gene cDNA ORF clone, Homo sapiens(human)

mRNA	Protein	Name
XM_011509090.2	XP_011507392.1	lysine-specific demethylase 5B isoform X2
XM_011509091.2	XP_011507393.1	lysine-specific demethylase 5B isoform X1
NM_001314042.1	NP_001300971.1	lysine-specific demethylase 5B isoform 1
NM_006618.4	NP_006609.3	lysine-specific demethylase 5B isoform 2
XM_011509090.3	XP_011507392.1	lysine-specific demethylase 5B isoform X1
XM_011509092.2	XP_011507394.1	lysine-specific demethylase 5B isoform X2
NM_006618.5	NP_006609.3	lysine-specific demethylase 5B isoform 2
NM_006618.3	NP_006609.3	lysine-specific demethylase 5B
NM_001347591.1	NP_001334520.1	lysine-specific demethylase 5B isoform 3

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu27995	NM_006618.5 Latest version!	Homo sapiens lysine demethylase 5B (KDM5B), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$659.50-$923.30 $1319.00
OHu64356	NM_001314042.1 Latest version!	Homo sapiens lysine demethylase 5B (KDM5B), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$579.50-$811.30 $1159.00
OHu64359	XM_011509091.2 Latest version!	Homo sapiens lysine demethylase 5B (KDM5B), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$519.50-$727.30 $1039.00
OHu64359	XM_011509090.3 Latest version!	Homo sapiens lysine demethylase 5B (KDM5B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$519.50-$727.30 $1039.00
OHu64360	XM_011509092.2 Latest version!	Homo sapiens lysine demethylase 5B (KDM5B), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$727.30 $1039.00
OHu64357	NM_001347591.1 Latest version!	Homo sapiens lysine demethylase 5B (KDM5B), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
View Old Accession Versions >>
OHu64359	XM_011509090.2	Homo sapiens lysine demethylase 5B (KDM5B), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$519.50-$727.30 $1039.00
OHu27995	NM_006618.4	Homo sapiens lysine demethylase 5B (KDM5B), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$659.50-$923.30 $1319.00
OHu27995	NM_006618.3	Homo sapiens lysine (K)-specific demethylase 5B (KDM5B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$659.50-$923.30 $1319.00
Hide Old Accession Versions >>

KDM5B ( NM_006618.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_006609.3 Homo sapiens lysine demethylase 5B (KDM5B), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu27995
Clone ID Related Accession (Same CDS sequence)	NM_006618.4 , NM_006618.5 , NM_006618.3
Accession Version	NM_006618.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4635bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-05
Organism	Homo sapiens(human)
Product	lysine-specific demethylase 5B isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC098934.3, DA436869.1, AJ132440.1, AC104463.3 and AI262935.1. On Sep 26, 2015 this sequence version replaced NM_006618.3. Transcript Variant: This variant (2) lacks an exon in the 5' coding region compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AJ243706.1, SRR1803613.155389.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: upstream AUG and CDS extension is not conserved RefSeq Select criteria :: based on manual assertion, conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGAGGCGG CCACCACACT GCACCCAGGC CCGCGCCCGG CGCTGCCCCT CGGGGGCCCG 
GGCCCGCTGG GCGAGTTCCT GCCTCCACCC GAGTGCCCGG TCTTCGAACC CAGCTGGGAA 
GAGTTCGCGG ACCCCTTCGC TTTCATCCAC AAGATCCGGC CCATAGCCGA GCAGACTGGC 
ATCTGTAAGG TGCGGCCGCC GCCGGATTGG CAGCCACCAT TTGCATGTGA TGTTGATAAA 
CTTCATTTTA CGCCACGTAT CCAGAGACTG AATGAATTGG AGGCCCAAAC TCGTGTAAAA 
TTGAATTTCT TGGACCAGAT TGCAAAGTAC TGGGAGTTAC AGGGAAGTAC TCTGAAAATT 
CCACATGTGG AGAGGAAGAT CTTGGACTTA TTTCAGCTTA ATAAGTTAGT TGCAGAAGAA 
GGTGGATTTG CAGTTGTTTG CAAGGATAGA AAATGGACCA AAATTGCTAC CAAGATGGGG 
TTTGCTCCTG GCAAAGCAGT GGGCTCACAT ATCAGAGGGC ATTATGAACG AATTCTCAAC 
CCCTACAACT TATTCCTGTC CGGAGACAGC CTAAGGTGTT TGCAGAAGCC AAACCTGACC 
ACAGACACTA AGGACAAGGA GTACAAACCC CATGATATTC CCCAGAGGCA GTCTGTGCAG 
CCTTCGGAAA CGTGCCCCCC AGCCCGACGA GCAAAACGCA TGAGAGCAGA GGCCATGAAT 
ATTAAAATAG AACCCGAGGA GACAACGGAA GCCAGAACTC ATAATCTGAG ACGTCGAATG 
GGTTGTCCAA CTCCAAAATG TGAAAATGAG AAAGAAATGA AGAGTAGCAT CAAGCAAGAA 
CCTATTGAGA GGAAAGATTA TATTGTAGAA AATGAGAAGG AAAAGCCCAA GAGTCGATCT 
AAAAAAGCCA CCAATGCTGT GGACCTGTAT GTCTGTCTTT TATGTGGCAG TGGCAATGAT 
GAAGACCGGC TACTGTTGTG TGATGGCTGT GATGACAGTT ACCATACCTT TTGCTTGATC 
CCACCTCTCC ATGATGTTCC CAAGGGAGAC TGGAGGTGTC CTAAGTGTTT GGCTCAGGAA 
TGTAGTAAGC CACAAGAAGC ATTTGGCTTT GAACAAGCAG CCAGGGACTA TACCCTCCGT 
ACTTTTGGGG AAATGGCAGA TGCGTTCAAA TCTGATTACT TCAACATGCC AGTCCATATG 
GTCCCCACAG AGCTTGTTGA GAAAGAATTT TGGAGACTAG TAAGCACTAT TGAGGAGGAT 
GTCACAGTGG AATATGGAGC TGACATTGCC TCAAAGGAAT TTGGCAGTGG CTTTCCTGTC 
CGAGATGGGA AAATCAAACT CTCACCTGAG GAAGAGGAGT ATCTTGATAG TGGCTGGAAT 
TTGAACAACA TGCCAGTGAT GGAGCAGTCT GTCCTTGCAC ATATTACTGC TGATATATGT 
GGCATGAAAC TTCCTTGGTT GTATGTGGGA ATGTGCTTTT CTTCATTCTG TTGGCACATT 
GAAGACCACT GGAGCTATTC AATTAACTAC TTGCACTGGG GTGAGCCAAA AACCTGGTAT 
GGAGTCCCAG GGTATGCTGC TGAGCAGCTA GAAAATGTAA TGAAGAAACT AGCTCCAGAA 
CTCTTTGTGT CCCAGCCGGA TCTCCTCCAT CAGCTTGTGA CCATCATGAA CCCCAATACC 
CTGATGACTC ATGAAGTGCC TGTTTACCGA ACTAATCAGT GTGCTGGGGA GTTTGTGATT 
ACATTTCCAA GAGCCTACCA CAGTGGTTTT AACCAGGGTT TTAATTTTGC TGAGGCTGTT 
AACTTCTGCA CTGTTGATTG GCTGCCATTA GGCCGACAGT GTGTGGAGCA TTATCGCTTG 
CTTCATCGAT ATTGTGTGTT TTCCCACGAT GAGATGATCT GCAAGATGGC TTCCAAGGCT 
GATGTATTAG ATGTTGTAGT GGCTTCAACT GTTCAGAAAG ACATGGCCAT TATGATTGAG 
GATGAGAAAG CTTTAAGAGA AACTGTCCGT AAATTGGGAG TGATTGATTC GGAAAGAATG 
GATTTTGAGC TGTTGCCAGA TGATGAACGT CAGTGTGTAA AATGCAAAAC TACATGCTTC 
ATGTCTGCCA TCTCCTGTTC TTGTAAACCT GGCCTTCTTG TTTGCCTGCA TCATGTAAAA 
GAATTGTGTT CCTGTCCTCC TTACAAATAT AAATTGCGGT ATAGGTACAC GCTGGATGAT 
CTCTACCCTA TGATGAATGC ATTGAAGCTT CGAGCAGAAT CTTACAACGA ATGGGCCTTG 
AATGTGAATG AAGCTTTGGA GGCAAAGATC AACAAGAAGA AAAGCCTTGT CAGCTTCAAG 
GCTTTAATTG AAGAATCTGA AATGAAGAAA TTCCCAGACA ATGATCTTTT GCGACACCTT 
CGCCTAGTCA CACAGGATGC AGAGAAGTGT GCCTCTGTTG CGCAGCAGTT GCTTAATGGC 
AAAAGGCAAA CTAGATATCG ATCTGGTGGA GGGAAATCCC AAAATCAGTT GACAGTGAAT 
GAGCTCCGGC AGTTTGTAAC ACAGCTGTAT GCTCTTCCAT GTGTCCTCAG TCAGACACCA 
TTACTAAAGG ATCTCTTGAA TCGTGTAGAA GATTTTCAAC AGCATAGTCA GAAACTACTC 
TCTGAGGAAA CGCCTAGTGC TGCGGAGCTG CAGGACTTGC TAGATGTCAG CTTTGAATTT 
GATGTTGAAC TTCCACAGCT TGCTGAGATG CGTATCCGTT TGGAACAAGC CCGTTGGCTA 
GAAGAGGTGC AGCAAGCTTG CCTAGACCCC AGCTCCCTTA CTTTAGATGA TATGAGACGT 
CTCATAGACC TAGGGGTAGG GCTGGCCCCG TATTCAGCAG TGGAGAAAGC TATGGCCCGG 
CTGCAGGAAC TGCTCACAGT GTCAGAGCAC TGGGACGACA AAGCCAAGAG TCTCCTCAAG 
GCCAGGCCAC GACATTCATT GAATAGCCTT GCTACGGCAG TAAAGGAAAT CGAAGAGATC 
CCTGCATATC TGCCCAATGG TGCGGCTCTG AAAGACTCAG TGCAGAGAGC CAGAGACTGG 
CTTCAGGATG TAGAGGGCCT GCAGGCTGGA GGACGTGTGC CAGTGTTAGA CACACTCATA 
GAACTTGTTA CACGAGGCCG ATCTATCCCC GTACATCTGA ATTCTTTGCC AAGACTGGAA 
ACCCTAGTAG CTGAGGTTCA GGCTTGGAAA GAATGTGCTG TTAATACATT CTTGACTGAG 
AATTCTCCAT ATTCTCTCTT AGAGGTGCTG TGTCCTCGAT GTGATATTGG CCTTTTGGGA 
TTGAAAAGGA AGCAGAGAAA GTTAAAGGAG CCCTTGCCAA ATGGAAAGAA AAAAAGCACC 
AAATTAGAGA GTCTGAGTGA CCTGGAGAGA GCTTTAACTG AAAGCAAGGA GACTGCTTCA 
GCTATGGCAA CTCTTGGGGA AGCTCGCCTA AGGGAAATGG AAGCCTTGCA GTCTCTCAGA 
CTCGCCAATG AAGGGAAATT GCTGTCGCCT CTCCAAGATG TGGATATAAA AATCTGCCTA 
TGTCAGAAGG CCCCAGCTGC CCCTATGATT CAATGTGAAC TCTGCAGGGA TGCTTTCCAC 
ACCAGTTGTG TGGCGGTACC CAGTATTTCA CAGGGCCTGC GAATCTGGCT TTGTCCCCAT 
TGTCGGAGGT CAGAGAAACC TCCATTAGAG AAAATTCTGC CCCTGCTCGC CTCCCTTCAG 
CGTATCCGAG TTCGCCTTCC TGAGGGAGAT GCACTTCGAT ATATGATTGA AAGAACCGTG 
AACTGGCAGC ACAGAGCCCA GCAACTGCTT TCGTCAGGGA ATCTTAAATT TGTGCAAGAT 
CGAGTGGGCT CAGGACTGTT ATATAGCAGA TGGCAAGCCT CAGCAGGACA GGTGTCAGAC 
ACAAACAAGG TATCTCAACC TCCTGGCACA ACATCATTTT CTTTGCCTGA TGACTGGGAC 
AACAGAACCT CATATTTGCA CTCCCCCTTC TCAACTGGAC GAAGTTGTAT CCCCCTCCAT 
GGTGTTAGTC CAGAAGTGAA TGAACTATTG ATGGAAGCCC AGCTGCTCCA GGTATCCCTT 
CCTGAAATTC AGGAACTTTA CCAGACTTTA CTTGCAAAGC CAAGCCCTGC TCAGCAGACT 
GACCGAAGCT CACCAGTGAG ACCCAGCAGT GAGAAGAATG ACTGTTGCCG AGGGAAGCGA 
GATGGAATTA ACAGTCTTGA GAGAAAACTG AAGAGACGCC TGGAAAGAGA GGGCCTCTCC 
AGTGAGCGGT GGGAACGAGT TAAGAAAATG CGGACCCCCA AAAAGAAGAA AATCAAACTG 
AGCCACCCCA AGGACATGAA CAATTTCAAG TTAGAGAGAG AGCGTAGCTA TGAATTAGTT 
CGTTCTGCTG AAACTCATTC CCTGCCCTCA GACACATCCT ATTCCGAACA GGAAGACTCT 
GAGGATGAAG ATGCCATCTG CCCAGCTGTG AGCTGCCTGC AGCCAGAAGG AGATGAGGTG 
GACTGGGTCC AGTGTGATGG CAGCTGCAAT CAGTGGTTTC ATCAGGTCTG TGTTGGTGTC 
TCCCCAGAGA TGGCAGAGAA AGAAGACTAC ATCTGTGTGC GCTGTACTGT GAAGGACGCA 
CCAAGCCGAA AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_006609.3
CDS	1166..5800
Translation	MEAATTLHPGPRPALPLGGPGPLGEFLPPPECPVFEPSWEEFADPFAFIHKIRPIAEQTGICKVRPPPDWQPPFACDVDKLHFTPRIQRLNELEAQTRVKLNFLDQIAKYWELQGSTLKIPHVERKILDLFQLNKLVAEEGGFAVVCKDRKWTKIATKMGFAPGKAVGSHIRGHYERILNPYNLFLSGDSLRCLQKPNLTTDTKDKEYKPHDIPQRQSVQPSETCPPARRAKRMRAEAMNIKIEPEETTEARTHNLRRRMGCPTPKCENEKEMKSSIKQEPIERKDYIVENEKEKPKSRSKKATNAVDLYVCLLCGSGNDEDRLLLCDGCDDSYHTFCLIPPLHDVPKGDWRCPKCLAQECSKPQEAFGFEQAARDYTLRTFGEMADAFKSDYFNMPVHMVPTELVEKEFWRLVSTIEEDVTVEYGADIASKEFGSGFPVRDGKIKLSPEEEEYLDSGWNLNNMPVMEQSVLAHITADICGMKLPWLYVGMCFSSFCWHIEDHWSYSINYLHWGEPKTWYGVPGYAAEQLENVMKKLAPELFVSQPDLLHQLVTIMNPNTLMTHEVPVYRTNQCAGEFVITFPRAYHSGFNQGFNFAEAVNFCTVDWLPLGRQCVEHYRLLHRYCVFSHDEMICKMASKADVLDVVVASTVQKDMAIMIEDEKALRETVRKLGVIDSERMDFELLPDDERQCVKCKTTCFMSAISCSCKPGLLVCLHHVKELCSCPPYKYKLRYRYTLDDLYPMMNALKLRAESYNEWALNVNEALEAKINKKKSLVSFKALIEESEMKKFPDNDLLRHLRLVTQDAEKCASVAQQLLNGKRQTRYRSGGGKSQNQLTVNELRQFVTQLYALPCVLSQTPLLKDLLNRVEDFQQHSQKLLSEETPSAAELQDLLDVSFEFDVELPQLAEMRIRLEQARWLEEVQQACLDPSSLTLDDMRRLIDLGVGLAPYSAVEKAMARLQELLTVSEHWDDKAKSLLKARPRHSLNSLATAVKEIEEIPAYLPNGAALKDSVQRARDWLQDVEGLQAGGRVPVLDTLIELVTRGRSIPVHLNSLPRLETLVAEVQAWKECAVNTFLTENSPYSLLEVLCPRCDIGLLGLKRKQRKLKEPLPNGKKKSTKLESLSDLERALTESKETASAMATLGEARLREMEALQSLRLANEGKLLSPLQDVDIKICLCQKAPAAPMIQCELCRDAFHTSCVAVPSISQGLRIWLCPHCRRSEKPPLEKILPLLASLQRIRVRLPEGDALRYMIERTVNWQHRAQQLLSSGNLKFVQDRVGSGLLYSRWQASAGQVSDTNKVSQPPGTTSFSLPDDWDNRTSYLHSPFSTGRSCIPLHGVSPEVNELLMEAQLLQVSLPEIQELYQTLLAKPSPAQQTDRSSPVRPSSEKNDCCRGKRDGINSLERKLKRRLEREGLSSERWERVKKMRTPKKKKIKLSHPKDMNNFKLERERSYELVRSAETHSLPSDTSYSEQEDSEDEDAICPAVSCLQPEGDEVDWVQCDGSCNQWFHQVCVGVSPEMAEKEDYICVRCTVKDAPSRK

Target ORF information:

RefSeq Version	NM_006618.4
Organism	Homo sapiens(human)
Definition	Homo sapiens lysine demethylase 5B (KDM5B), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006618.4

ORF Insert Sequence:

ATGGAGGCGG CCACCACACT GCACCCAGGC CCGCGCCCGG CGCTGCCCCT CGGGGGCCCG 
GGCCCGCTGG GCGAGTTCCT GCCTCCACCC GAGTGCCCGG TCTTCGAACC CAGCTGGGAA 
GAGTTCGCGG ACCCCTTCGC TTTCATCCAC AAGATCCGGC CCATAGCCGA GCAGACTGGC 
ATCTGTAAGG TGCGGCCGCC GCCGGATTGG CAGCCACCAT TTGCATGTGA TGTTGATAAA 
CTTCATTTTA CGCCACGTAT CCAGAGACTG AATGAATTGG AGGCCCAAAC TCGTGTAAAA 
TTGAATTTCT TGGACCAGAT TGCAAAGTAC TGGGAGTTAC AGGGAAGTAC TCTGAAAATT 
CCACATGTGG AGAGGAAGAT CTTGGACTTA TTTCAGCTTA ATAAGTTAGT TGCAGAAGAA 
GGTGGATTTG CAGTTGTTTG CAAGGATAGA AAATGGACCA AAATTGCTAC CAAGATGGGG 
TTTGCTCCTG GCAAAGCAGT GGGCTCACAT ATCAGAGGGC ATTATGAACG AATTCTCAAC 
CCCTACAACT TATTCCTGTC CGGAGACAGC CTAAGGTGTT TGCAGAAGCC AAACCTGACC 
ACAGACACTA AGGACAAGGA GTACAAACCC CATGATATTC CCCAGAGGCA GTCTGTGCAG 
CCTTCGGAAA CGTGCCCCCC AGCCCGACGA GCAAAACGCA TGAGAGCAGA GGCCATGAAT 
ATTAAAATAG AACCCGAGGA GACAACGGAA GCCAGAACTC ATAATCTGAG ACGTCGAATG 
GGTTGTCCAA CTCCAAAATG TGAAAATGAG AAAGAAATGA AGAGTAGCAT CAAGCAAGAA 
CCTATTGAGA GGAAAGATTA TATTGTAGAA AATGAGAAGG AAAAGCCCAA GAGTCGATCT 
AAAAAAGCCA CCAATGCTGT GGACCTGTAT GTCTGTCTTT TATGTGGCAG TGGCAATGAT 
GAAGACCGGC TACTGTTGTG TGATGGCTGT GATGACAGTT ACCATACCTT TTGCTTGATC 
CCACCTCTCC ATGATGTTCC CAAGGGAGAC TGGAGGTGTC CTAAGTGTTT GGCTCAGGAA 
TGTAGTAAGC CACAAGAAGC ATTTGGCTTT GAACAAGCAG CCAGGGACTA TACCCTCCGT 
ACTTTTGGGG AAATGGCAGA TGCGTTCAAA TCTGATTACT TCAACATGCC AGTCCATATG 
GTCCCCACAG AGCTTGTTGA GAAAGAATTT TGGAGACTAG TAAGCACTAT TGAGGAGGAT 
GTCACAGTGG AATATGGAGC TGACATTGCC TCAAAGGAAT TTGGCAGTGG CTTTCCTGTC 
CGAGATGGGA AAATCAAACT CTCACCTGAG GAAGAGGAGT ATCTTGATAG TGGCTGGAAT 
TTGAACAACA TGCCAGTGAT GGAGCAGTCT GTCCTTGCAC ATATTACTGC TGATATATGT 
GGCATGAAAC TTCCTTGGTT GTATGTGGGA ATGTGCTTTT CTTCATTCTG TTGGCACATT 
GAAGACCACT GGAGCTATTC AATTAACTAC TTGCACTGGG GTGAGCCAAA AACCTGGTAT 
GGAGTCCCAG GGTATGCTGC TGAGCAGCTA GAAAATGTAA TGAAGAAACT AGCTCCAGAA 
CTCTTTGTGT CCCAGCCGGA TCTCCTCCAT CAGCTTGTGA CCATCATGAA CCCCAATACC 
CTGATGACTC ATGAAGTGCC TGTTTACCGA ACTAATCAGT GTGCTGGGGA GTTTGTGATT 
ACATTTCCAA GAGCCTACCA CAGTGGTTTT AACCAGGGTT TTAATTTTGC TGAGGCTGTT 
AACTTCTGCA CTGTTGATTG GCTGCCATTA GGCCGACAGT GTGTGGAGCA TTATCGCTTG 
CTTCATCGAT ATTGTGTGTT TTCCCACGAT GAGATGATCT GCAAGATGGC TTCCAAGGCT 
GATGTATTAG ATGTTGTAGT GGCTTCAACT GTTCAGAAAG ACATGGCCAT TATGATTGAG 
GATGAGAAAG CTTTAAGAGA AACTGTCCGT AAATTGGGAG TGATTGATTC GGAAAGAATG 
GATTTTGAGC TGTTGCCAGA TGATGAACGT CAGTGTGTAA AATGCAAAAC TACATGCTTC 
ATGTCTGCCA TCTCCTGTTC TTGTAAACCT GGCCTTCTTG TTTGCCTGCA TCATGTAAAA 
GAATTGTGTT CCTGTCCTCC TTACAAATAT AAATTGCGGT ATAGGTACAC GCTGGATGAT 
CTCTACCCTA TGATGAATGC ATTGAAGCTT CGAGCAGAAT CTTACAACGA ATGGGCCTTG 
AATGTGAATG AAGCTTTGGA GGCAAAGATC AACAAGAAGA AAAGCCTTGT CAGCTTCAAG 
GCTTTAATTG AAGAATCTGA AATGAAGAAA TTCCCAGACA ATGATCTTTT GCGACACCTT 
CGCCTAGTCA CACAGGATGC AGAGAAGTGT GCCTCTGTTG CGCAGCAGTT GCTTAATGGC 
AAAAGGCAAA CTAGATATCG ATCTGGTGGA GGGAAATCCC AAAATCAGTT GACAGTGAAT 
GAGCTCCGGC AGTTTGTAAC ACAGCTGTAT GCTCTTCCAT GTGTCCTCAG TCAGACACCA 
TTACTAAAGG ATCTCTTGAA TCGTGTAGAA GATTTTCAAC AGCATAGTCA GAAACTACTC 
TCTGAGGAAA CGCCTAGTGC TGCGGAGCTG CAGGACTTGC TAGATGTCAG CTTTGAATTT 
GATGTTGAAC TTCCACAGCT TGCTGAGATG CGTATCCGTT TGGAACAAGC CCGTTGGCTA 
GAAGAGGTGC AGCAAGCTTG CCTAGACCCC AGCTCCCTTA CTTTAGATGA TATGAGACGT 
CTCATAGACC TAGGGGTAGG GCTGGCCCCG TATTCAGCAG TGGAGAAAGC TATGGCCCGG 
CTGCAGGAAC TGCTCACAGT GTCAGAGCAC TGGGACGACA AAGCCAAGAG TCTCCTCAAG 
GCCAGGCCAC GACATTCATT GAATAGCCTT GCTACGGCAG TAAAGGAAAT CGAAGAGATC 
CCTGCATATC TGCCCAATGG TGCGGCTCTG AAAGACTCAG TGCAGAGAGC CAGAGACTGG 
CTTCAGGATG TAGAGGGCCT GCAGGCTGGA GGACGTGTGC CAGTGTTAGA CACACTCATA 
GAACTTGTTA CACGAGGCCG ATCTATCCCC GTACATCTGA ATTCTTTGCC AAGACTGGAA 
ACCCTAGTAG CTGAGGTTCA GGCTTGGAAA GAATGTGCTG TTAATACATT CTTGACTGAG 
AATTCTCCAT ATTCTCTCTT AGAGGTGCTG TGTCCTCGAT GTGATATTGG CCTTTTGGGA 
TTGAAAAGGA AGCAGAGAAA GTTAAAGGAG CCCTTGCCAA ATGGAAAGAA AAAAAGCACC 
AAATTAGAGA GTCTGAGTGA CCTGGAGAGA GCTTTAACTG AAAGCAAGGA GACTGCTTCA 
GCTATGGCAA CTCTTGGGGA AGCTCGCCTA AGGGAAATGG AAGCCTTGCA GTCTCTCAGA 
CTCGCCAATG AAGGGAAATT GCTGTCGCCT CTCCAAGATG TGGATATAAA AATCTGCCTA 
TGTCAGAAGG CCCCAGCTGC CCCTATGATT CAATGTGAAC TCTGCAGGGA TGCTTTCCAC 
ACCAGTTGTG TGGCGGTACC CAGTATTTCA CAGGGCCTGC GAATCTGGCT TTGTCCCCAT 
TGTCGGAGGT CAGAGAAACC TCCATTAGAG AAAATTCTGC CCCTGCTCGC CTCCCTTCAG 
CGTATCCGAG TTCGCCTTCC TGAGGGAGAT GCACTTCGAT ATATGATTGA AAGAACCGTG 
AACTGGCAGC ACAGAGCCCA GCAACTGCTT TCGTCAGGGA ATCTTAAATT TGTGCAAGAT 
CGAGTGGGCT CAGGACTGTT ATATAGCAGA TGGCAAGCCT CAGCAGGACA GGTGTCAGAC 
ACAAACAAGG TATCTCAACC TCCTGGCACA ACATCATTTT CTTTGCCTGA TGACTGGGAC 
AACAGAACCT CATATTTGCA CTCCCCCTTC TCAACTGGAC GAAGTTGTAT CCCCCTCCAT 
GGTGTTAGTC CAGAAGTGAA TGAACTATTG ATGGAAGCCC AGCTGCTCCA GGTATCCCTT 
CCTGAAATTC AGGAACTTTA CCAGACTTTA CTTGCAAAGC CAAGCCCTGC TCAGCAGACT 
GACCGAAGCT CACCAGTGAG ACCCAGCAGT GAGAAGAATG ACTGTTGCCG AGGGAAGCGA 
GATGGAATTA ACAGTCTTGA GAGAAAACTG AAGAGACGCC TGGAAAGAGA GGGCCTCTCC 
AGTGAGCGGT GGGAACGAGT TAAGAAAATG CGGACCCCCA AAAAGAAGAA AATCAAACTG 
AGCCACCCCA AGGACATGAA CAATTTCAAG TTAGAGAGAG AGCGTAGCTA TGAATTAGTT 
CGTTCTGCTG AAACTCATTC CCTGCCCTCA GACACATCCT ATTCCGAACA GGAAGACTCT 
GAGGATGAAG ATGCCATCTG CCCAGCTGTG AGCTGCCTGC AGCCAGAAGG AGATGAGGTG 
GACTGGGTCC AGTGTGATGG CAGCTGCAAT CAGTGGTTTC ATCAGGTCTG TGTTGGTGTC 
TCCCCAGAGA TGGCAGAGAA AGAAGACTAC ATCTGTGTGC GCTGTACTGT GAAGGACGCA 
CCAAGCCGAA AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM5B ( NM_006618.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_006609.3 Homo sapiens lysine demethylase 5B (KDM5B), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu27995
Clone ID Related Accession (Same CDS sequence)	NM_006618.4 , NM_006618.5 , NM_006618.3
Accession Version	NM_006618.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4635bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-09
Organism	Homo sapiens(human)
Product	lysine-specific demethylase 5B isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC098934.3 and AC104463.3. On Jul 26, 2019 this sequence version replaced NM_006618.4. Transcript Variant: This variant (2) lacks an exon in the 5' coding region compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.56526.1, SRR1803613.81220.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on manual assertion, conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGAGGCGG CCACCACACT GCACCCAGGC CCGCGCCCGG CGCTGCCCCT CGGGGGCCCG 
GGCCCGCTGG GCGAGTTCCT GCCTCCACCC GAGTGCCCGG TCTTCGAACC CAGCTGGGAA 
GAGTTCGCGG ACCCCTTCGC TTTCATCCAC AAGATCCGGC CCATAGCCGA GCAGACTGGC 
ATCTGTAAGG TGCGGCCGCC GCCGGATTGG CAGCCACCAT TTGCATGTGA TGTTGATAAA 
CTTCATTTTA CGCCACGTAT CCAGAGACTG AATGAATTGG AGGCCCAAAC TCGTGTAAAA 
TTGAATTTCT TGGACCAGAT TGCAAAGTAC TGGGAGTTAC AGGGAAGTAC TCTGAAAATT 
CCACATGTGG AGAGGAAGAT CTTGGACTTA TTTCAGCTTA ATAAGTTAGT TGCAGAAGAA 
GGTGGATTTG CAGTTGTTTG CAAGGATAGA AAATGGACCA AAATTGCTAC CAAGATGGGG 
TTTGCTCCTG GCAAAGCAGT GGGCTCACAT ATCAGAGGGC ATTATGAACG AATTCTCAAC 
CCCTACAACT TATTCCTGTC CGGAGACAGC CTAAGGTGTT TGCAGAAGCC AAACCTGACC 
ACAGACACTA AGGACAAGGA GTACAAACCC CATGATATTC CCCAGAGGCA GTCTGTGCAG 
CCTTCGGAAA CGTGCCCCCC AGCCCGACGA GCAAAACGCA TGAGAGCAGA GGCCATGAAT 
ATTAAAATAG AACCCGAGGA GACAACGGAA GCCAGAACTC ATAATCTGAG ACGTCGAATG 
GGTTGTCCAA CTCCAAAATG TGAAAATGAG AAAGAAATGA AGAGTAGCAT CAAGCAAGAA 
CCTATTGAGA GGAAAGATTA TATTGTAGAA AATGAGAAGG AAAAGCCCAA GAGTCGATCT 
AAAAAAGCCA CCAATGCTGT GGACCTGTAT GTCTGTCTTT TATGTGGCAG TGGCAATGAT 
GAAGACCGGC TACTGTTGTG TGATGGCTGT GATGACAGTT ACCATACCTT TTGCTTGATC 
CCACCTCTCC ATGATGTTCC CAAGGGAGAC TGGAGGTGTC CTAAGTGTTT GGCTCAGGAA 
TGTAGTAAGC CACAAGAAGC ATTTGGCTTT GAACAAGCAG CCAGGGACTA TACCCTCCGT 
ACTTTTGGGG AAATGGCAGA TGCGTTCAAA TCTGATTACT TCAACATGCC AGTCCATATG 
GTCCCCACAG AGCTTGTTGA GAAAGAATTT TGGAGACTAG TAAGCACTAT TGAGGAGGAT 
GTCACAGTGG AATATGGAGC TGACATTGCC TCAAAGGAAT TTGGCAGTGG CTTTCCTGTC 
CGAGATGGGA AAATCAAACT CTCACCTGAG GAAGAGGAGT ATCTTGATAG TGGCTGGAAT 
TTGAACAACA TGCCAGTGAT GGAGCAGTCT GTCCTTGCAC ATATTACTGC TGATATATGT 
GGCATGAAAC TTCCTTGGTT GTATGTGGGA ATGTGCTTTT CTTCATTCTG TTGGCACATT 
GAAGACCACT GGAGCTATTC AATTAACTAC TTGCACTGGG GTGAGCCAAA AACCTGGTAT 
GGAGTCCCAG GGTATGCTGC TGAGCAGCTA GAAAATGTAA TGAAGAAACT AGCTCCAGAA 
CTCTTTGTGT CCCAGCCGGA TCTCCTCCAT CAGCTTGTGA CCATCATGAA CCCCAATACC 
CTGATGACTC ATGAAGTGCC TGTTTACCGA ACTAATCAGT GTGCTGGGGA GTTTGTGATT 
ACATTTCCAA GAGCCTACCA CAGTGGTTTT AACCAGGGTT TTAATTTTGC TGAGGCTGTT 
AACTTCTGCA CTGTTGATTG GCTGCCATTA GGCCGACAGT GTGTGGAGCA TTATCGCTTG 
CTTCATCGAT ATTGTGTGTT TTCCCACGAT GAGATGATCT GCAAGATGGC TTCCAAGGCT 
GATGTATTAG ATGTTGTAGT GGCTTCAACT GTTCAGAAAG ACATGGCCAT TATGATTGAG 
GATGAGAAAG CTTTAAGAGA AACTGTCCGT AAATTGGGAG TGATTGATTC GGAAAGAATG 
GATTTTGAGC TGTTGCCAGA TGATGAACGT CAGTGTGTAA AATGCAAAAC TACATGCTTC 
ATGTCTGCCA TCTCCTGTTC TTGTAAACCT GGCCTTCTTG TTTGCCTGCA TCATGTAAAA 
GAATTGTGTT CCTGTCCTCC TTACAAATAT AAATTGCGGT ATAGGTACAC GCTGGATGAT 
CTCTACCCTA TGATGAATGC ATTGAAGCTT CGAGCAGAAT CTTACAACGA ATGGGCCTTG 
AATGTGAATG AAGCTTTGGA GGCAAAGATC AACAAGAAGA AAAGCCTTGT CAGCTTCAAG 
GCTTTAATTG AAGAATCTGA AATGAAGAAA TTCCCAGACA ATGATCTTTT GCGACACCTT 
CGCCTAGTCA CACAGGATGC AGAGAAGTGT GCCTCTGTTG CGCAGCAGTT GCTTAATGGC 
AAAAGGCAAA CTAGATATCG ATCTGGTGGA GGGAAATCCC AAAATCAGTT GACAGTGAAT 
GAGCTCCGGC AGTTTGTAAC ACAGCTGTAT GCTCTTCCAT GTGTCCTCAG TCAGACACCA 
TTACTAAAGG ATCTCTTGAA TCGTGTAGAA GATTTTCAAC AGCATAGTCA GAAACTACTC 
TCTGAGGAAA CGCCTAGTGC TGCGGAGCTG CAGGACTTGC TAGATGTCAG CTTTGAATTT 
GATGTTGAAC TTCCACAGCT TGCTGAGATG CGTATCCGTT TGGAACAAGC CCGTTGGCTA 
GAAGAGGTGC AGCAAGCTTG CCTAGACCCC AGCTCCCTTA CTTTAGATGA TATGAGACGT 
CTCATAGACC TAGGGGTAGG GCTGGCCCCG TATTCAGCAG TGGAGAAAGC TATGGCCCGG 
CTGCAGGAAC TGCTCACAGT GTCAGAGCAC TGGGACGACA AAGCCAAGAG TCTCCTCAAG 
GCCAGGCCAC GACATTCATT GAATAGCCTT GCTACGGCAG TAAAGGAAAT CGAAGAGATC 
CCTGCATATC TGCCCAATGG TGCGGCTCTG AAAGACTCAG TGCAGAGAGC CAGAGACTGG 
CTTCAGGATG TAGAGGGCCT GCAGGCTGGA GGACGTGTGC CAGTGTTAGA CACACTCATA 
GAACTTGTTA CACGAGGCCG ATCTATCCCC GTACATCTGA ATTCTTTGCC AAGACTGGAA 
ACCCTAGTAG CTGAGGTTCA GGCTTGGAAA GAATGTGCTG TTAATACATT CTTGACTGAG 
AATTCTCCAT ATTCTCTCTT AGAGGTGCTG TGTCCTCGAT GTGATATTGG CCTTTTGGGA 
TTGAAAAGGA AGCAGAGAAA GTTAAAGGAG CCCTTGCCAA ATGGAAAGAA AAAAAGCACC 
AAATTAGAGA GTCTGAGTGA CCTGGAGAGA GCTTTAACTG AAAGCAAGGA GACTGCTTCA 
GCTATGGCAA CTCTTGGGGA AGCTCGCCTA AGGGAAATGG AAGCCTTGCA GTCTCTCAGA 
CTCGCCAATG AAGGGAAATT GCTGTCGCCT CTCCAAGATG TGGATATAAA AATCTGCCTA 
TGTCAGAAGG CCCCAGCTGC CCCTATGATT CAATGTGAAC TCTGCAGGGA TGCTTTCCAC 
ACCAGTTGTG TGGCGGTACC CAGTATTTCA CAGGGCCTGC GAATCTGGCT TTGTCCCCAT 
TGTCGGAGGT CAGAGAAACC TCCATTAGAG AAAATTCTGC CCCTGCTCGC CTCCCTTCAG 
CGTATCCGAG TTCGCCTTCC TGAGGGAGAT GCACTTCGAT ATATGATTGA AAGAACCGTG 
AACTGGCAGC ACAGAGCCCA GCAACTGCTT TCGTCAGGGA ATCTTAAATT TGTGCAAGAT 
CGAGTGGGCT CAGGACTGTT ATATAGCAGA TGGCAAGCCT CAGCAGGACA GGTGTCAGAC 
ACAAACAAGG TATCTCAACC TCCTGGCACA ACATCATTTT CTTTGCCTGA TGACTGGGAC 
AACAGAACCT CATATTTGCA CTCCCCCTTC TCAACTGGAC GAAGTTGTAT CCCCCTCCAT 
GGTGTTAGTC CAGAAGTGAA TGAACTATTG ATGGAAGCCC AGCTGCTCCA GGTATCCCTT 
CCTGAAATTC AGGAACTTTA CCAGACTTTA CTTGCAAAGC CAAGCCCTGC TCAGCAGACT 
GACCGAAGCT CACCAGTGAG ACCCAGCAGT GAGAAGAATG ACTGTTGCCG AGGGAAGCGA 
GATGGAATTA ACAGTCTTGA GAGAAAACTG AAGAGACGCC TGGAAAGAGA GGGCCTCTCC 
AGTGAGCGGT GGGAACGAGT TAAGAAAATG CGGACCCCCA AAAAGAAGAA AATCAAACTG 
AGCCACCCCA AGGACATGAA CAATTTCAAG TTAGAGAGAG AGCGTAGCTA TGAATTAGTT 
CGTTCTGCTG AAACTCATTC CCTGCCCTCA GACACATCCT ATTCCGAACA GGAAGACTCT 
GAGGATGAAG ATGCCATCTG CCCAGCTGTG AGCTGCCTGC AGCCAGAAGG AGATGAGGTG 
GACTGGGTCC AGTGTGATGG CAGCTGCAAT CAGTGGTTTC ATCAGGTCTG TGTTGGTGTC 
TCCCCAGAGA TGGCAGAGAA AGAAGACTAC ATCTGTGTGC GCTGTACTGT GAAGGACGCA 
CCAAGCCGAA AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_006609.3
CDS	117..4751
Translation	MEAATTLHPGPRPALPLGGPGPLGEFLPPPECPVFEPSWEEFADPFAFIHKIRPIAEQTGICKVRPPPDWQPPFACDVDKLHFTPRIQRLNELEAQTRVKLNFLDQIAKYWELQGSTLKIPHVERKILDLFQLNKLVAEEGGFAVVCKDRKWTKIATKMGFAPGKAVGSHIRGHYERILNPYNLFLSGDSLRCLQKPNLTTDTKDKEYKPHDIPQRQSVQPSETCPPARRAKRMRAEAMNIKIEPEETTEARTHNLRRRMGCPTPKCENEKEMKSSIKQEPIERKDYIVENEKEKPKSRSKKATNAVDLYVCLLCGSGNDEDRLLLCDGCDDSYHTFCLIPPLHDVPKGDWRCPKCLAQECSKPQEAFGFEQAARDYTLRTFGEMADAFKSDYFNMPVHMVPTELVEKEFWRLVSTIEEDVTVEYGADIASKEFGSGFPVRDGKIKLSPEEEEYLDSGWNLNNMPVMEQSVLAHITADICGMKLPWLYVGMCFSSFCWHIEDHWSYSINYLHWGEPKTWYGVPGYAAEQLENVMKKLAPELFVSQPDLLHQLVTIMNPNTLMTHEVPVYRTNQCAGEFVITFPRAYHSGFNQGFNFAEAVNFCTVDWLPLGRQCVEHYRLLHRYCVFSHDEMICKMASKADVLDVVVASTVQKDMAIMIEDEKALRETVRKLGVIDSERMDFELLPDDERQCVKCKTTCFMSAISCSCKPGLLVCLHHVKELCSCPPYKYKLRYRYTLDDLYPMMNALKLRAESYNEWALNVNEALEAKINKKKSLVSFKALIEESEMKKFPDNDLLRHLRLVTQDAEKCASVAQQLLNGKRQTRYRSGGGKSQNQLTVNELRQFVTQLYALPCVLSQTPLLKDLLNRVEDFQQHSQKLLSEETPSAAELQDLLDVSFEFDVELPQLAEMRIRLEQARWLEEVQQACLDPSSLTLDDMRRLIDLGVGLAPYSAVEKAMARLQELLTVSEHWDDKAKSLLKARPRHSLNSLATAVKEIEEIPAYLPNGAALKDSVQRARDWLQDVEGLQAGGRVPVLDTLIELVTRGRSIPVHLNSLPRLETLVAEVQAWKECAVNTFLTENSPYSLLEVLCPRCDIGLLGLKRKQRKLKEPLPNGKKKSTKLESLSDLERALTESKETASAMATLGEARLREMEALQSLRLANEGKLLSPLQDVDIKICLCQKAPAAPMIQCELCRDAFHTSCVAVPSISQGLRIWLCPHCRRSEKPPLEKILPLLASLQRIRVRLPEGDALRYMIERTVNWQHRAQQLLSSGNLKFVQDRVGSGLLYSRWQASAGQVSDTNKVSQPPGTTSFSLPDDWDNRTSYLHSPFSTGRSCIPLHGVSPEVNELLMEAQLLQVSLPEIQELYQTLLAKPSPAQQTDRSSPVRPSSEKNDCCRGKRDGINSLERKLKRRLEREGLSSERWERVKKMRTPKKKKIKLSHPKDMNNFKLERERSYELVRSAETHSLPSDTSYSEQEDSEDEDAICPAVSCLQPEGDEVDWVQCDGSCNQWFHQVCVGVSPEMAEKEDYICVRCTVKDAPSRK

Target ORF information:

RefSeq Version	NM_006618.5
Organism	Homo sapiens(human)
Definition	Homo sapiens lysine demethylase 5B (KDM5B), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006618.5

ORF Insert Sequence:

ATGGAGGCGG CCACCACACT GCACCCAGGC CCGCGCCCGG CGCTGCCCCT CGGGGGCCCG 
GGCCCGCTGG GCGAGTTCCT GCCTCCACCC GAGTGCCCGG TCTTCGAACC CAGCTGGGAA 
GAGTTCGCGG ACCCCTTCGC TTTCATCCAC AAGATCCGGC CCATAGCCGA GCAGACTGGC 
ATCTGTAAGG TGCGGCCGCC GCCGGATTGG CAGCCACCAT TTGCATGTGA TGTTGATAAA 
CTTCATTTTA CGCCACGTAT CCAGAGACTG AATGAATTGG AGGCCCAAAC TCGTGTAAAA 
TTGAATTTCT TGGACCAGAT TGCAAAGTAC TGGGAGTTAC AGGGAAGTAC TCTGAAAATT 
CCACATGTGG AGAGGAAGAT CTTGGACTTA TTTCAGCTTA ATAAGTTAGT TGCAGAAGAA 
GGTGGATTTG CAGTTGTTTG CAAGGATAGA AAATGGACCA AAATTGCTAC CAAGATGGGG 
TTTGCTCCTG GCAAAGCAGT GGGCTCACAT ATCAGAGGGC ATTATGAACG AATTCTCAAC 
CCCTACAACT TATTCCTGTC CGGAGACAGC CTAAGGTGTT TGCAGAAGCC AAACCTGACC 
ACAGACACTA AGGACAAGGA GTACAAACCC CATGATATTC CCCAGAGGCA GTCTGTGCAG 
CCTTCGGAAA CGTGCCCCCC AGCCCGACGA GCAAAACGCA TGAGAGCAGA GGCCATGAAT 
ATTAAAATAG AACCCGAGGA GACAACGGAA GCCAGAACTC ATAATCTGAG ACGTCGAATG 
GGTTGTCCAA CTCCAAAATG TGAAAATGAG AAAGAAATGA AGAGTAGCAT CAAGCAAGAA 
CCTATTGAGA GGAAAGATTA TATTGTAGAA AATGAGAAGG AAAAGCCCAA GAGTCGATCT 
AAAAAAGCCA CCAATGCTGT GGACCTGTAT GTCTGTCTTT TATGTGGCAG TGGCAATGAT 
GAAGACCGGC TACTGTTGTG TGATGGCTGT GATGACAGTT ACCATACCTT TTGCTTGATC 
CCACCTCTCC ATGATGTTCC CAAGGGAGAC TGGAGGTGTC CTAAGTGTTT GGCTCAGGAA 
TGTAGTAAGC CACAAGAAGC ATTTGGCTTT GAACAAGCAG CCAGGGACTA TACCCTCCGT 
ACTTTTGGGG AAATGGCAGA TGCGTTCAAA TCTGATTACT TCAACATGCC AGTCCATATG 
GTCCCCACAG AGCTTGTTGA GAAAGAATTT TGGAGACTAG TAAGCACTAT TGAGGAGGAT 
GTCACAGTGG AATATGGAGC TGACATTGCC TCAAAGGAAT TTGGCAGTGG CTTTCCTGTC 
CGAGATGGGA AAATCAAACT CTCACCTGAG GAAGAGGAGT ATCTTGATAG TGGCTGGAAT 
TTGAACAACA TGCCAGTGAT GGAGCAGTCT GTCCTTGCAC ATATTACTGC TGATATATGT 
GGCATGAAAC TTCCTTGGTT GTATGTGGGA ATGTGCTTTT CTTCATTCTG TTGGCACATT 
GAAGACCACT GGAGCTATTC AATTAACTAC TTGCACTGGG GTGAGCCAAA AACCTGGTAT 
GGAGTCCCAG GGTATGCTGC TGAGCAGCTA GAAAATGTAA TGAAGAAACT AGCTCCAGAA 
CTCTTTGTGT CCCAGCCGGA TCTCCTCCAT CAGCTTGTGA CCATCATGAA CCCCAATACC 
CTGATGACTC ATGAAGTGCC TGTTTACCGA ACTAATCAGT GTGCTGGGGA GTTTGTGATT 
ACATTTCCAA GAGCCTACCA CAGTGGTTTT AACCAGGGTT TTAATTTTGC TGAGGCTGTT 
AACTTCTGCA CTGTTGATTG GCTGCCATTA GGCCGACAGT GTGTGGAGCA TTATCGCTTG 
CTTCATCGAT ATTGTGTGTT TTCCCACGAT GAGATGATCT GCAAGATGGC TTCCAAGGCT 
GATGTATTAG ATGTTGTAGT GGCTTCAACT GTTCAGAAAG ACATGGCCAT TATGATTGAG 
GATGAGAAAG CTTTAAGAGA AACTGTCCGT AAATTGGGAG TGATTGATTC GGAAAGAATG 
GATTTTGAGC TGTTGCCAGA TGATGAACGT CAGTGTGTAA AATGCAAAAC TACATGCTTC 
ATGTCTGCCA TCTCCTGTTC TTGTAAACCT GGCCTTCTTG TTTGCCTGCA TCATGTAAAA 
GAATTGTGTT CCTGTCCTCC TTACAAATAT AAATTGCGGT ATAGGTACAC GCTGGATGAT 
CTCTACCCTA TGATGAATGC ATTGAAGCTT CGAGCAGAAT CTTACAACGA ATGGGCCTTG 
AATGTGAATG AAGCTTTGGA GGCAAAGATC AACAAGAAGA AAAGCCTTGT CAGCTTCAAG 
GCTTTAATTG AAGAATCTGA AATGAAGAAA TTCCCAGACA ATGATCTTTT GCGACACCTT 
CGCCTAGTCA CACAGGATGC AGAGAAGTGT GCCTCTGTTG CGCAGCAGTT GCTTAATGGC 
AAAAGGCAAA CTAGATATCG ATCTGGTGGA GGGAAATCCC AAAATCAGTT GACAGTGAAT 
GAGCTCCGGC AGTTTGTAAC ACAGCTGTAT GCTCTTCCAT GTGTCCTCAG TCAGACACCA 
TTACTAAAGG ATCTCTTGAA TCGTGTAGAA GATTTTCAAC AGCATAGTCA GAAACTACTC 
TCTGAGGAAA CGCCTAGTGC TGCGGAGCTG CAGGACTTGC TAGATGTCAG CTTTGAATTT 
GATGTTGAAC TTCCACAGCT TGCTGAGATG CGTATCCGTT TGGAACAAGC CCGTTGGCTA 
GAAGAGGTGC AGCAAGCTTG CCTAGACCCC AGCTCCCTTA CTTTAGATGA TATGAGACGT 
CTCATAGACC TAGGGGTAGG GCTGGCCCCG TATTCAGCAG TGGAGAAAGC TATGGCCCGG 
CTGCAGGAAC TGCTCACAGT GTCAGAGCAC TGGGACGACA AAGCCAAGAG TCTCCTCAAG 
GCCAGGCCAC GACATTCATT GAATAGCCTT GCTACGGCAG TAAAGGAAAT CGAAGAGATC 
CCTGCATATC TGCCCAATGG TGCGGCTCTG AAAGACTCAG TGCAGAGAGC CAGAGACTGG 
CTTCAGGATG TAGAGGGCCT GCAGGCTGGA GGACGTGTGC CAGTGTTAGA CACACTCATA 
GAACTTGTTA CACGAGGCCG ATCTATCCCC GTACATCTGA ATTCTTTGCC AAGACTGGAA 
ACCCTAGTAG CTGAGGTTCA GGCTTGGAAA GAATGTGCTG TTAATACATT CTTGACTGAG 
AATTCTCCAT ATTCTCTCTT AGAGGTGCTG TGTCCTCGAT GTGATATTGG CCTTTTGGGA 
TTGAAAAGGA AGCAGAGAAA GTTAAAGGAG CCCTTGCCAA ATGGAAAGAA AAAAAGCACC 
AAATTAGAGA GTCTGAGTGA CCTGGAGAGA GCTTTAACTG AAAGCAAGGA GACTGCTTCA 
GCTATGGCAA CTCTTGGGGA AGCTCGCCTA AGGGAAATGG AAGCCTTGCA GTCTCTCAGA 
CTCGCCAATG AAGGGAAATT GCTGTCGCCT CTCCAAGATG TGGATATAAA AATCTGCCTA 
TGTCAGAAGG CCCCAGCTGC CCCTATGATT CAATGTGAAC TCTGCAGGGA TGCTTTCCAC 
ACCAGTTGTG TGGCGGTACC CAGTATTTCA CAGGGCCTGC GAATCTGGCT TTGTCCCCAT 
TGTCGGAGGT CAGAGAAACC TCCATTAGAG AAAATTCTGC CCCTGCTCGC CTCCCTTCAG 
CGTATCCGAG TTCGCCTTCC TGAGGGAGAT GCACTTCGAT ATATGATTGA AAGAACCGTG 
AACTGGCAGC ACAGAGCCCA GCAACTGCTT TCGTCAGGGA ATCTTAAATT TGTGCAAGAT 
CGAGTGGGCT CAGGACTGTT ATATAGCAGA TGGCAAGCCT CAGCAGGACA GGTGTCAGAC 
ACAAACAAGG TATCTCAACC TCCTGGCACA ACATCATTTT CTTTGCCTGA TGACTGGGAC 
AACAGAACCT CATATTTGCA CTCCCCCTTC TCAACTGGAC GAAGTTGTAT CCCCCTCCAT 
GGTGTTAGTC CAGAAGTGAA TGAACTATTG ATGGAAGCCC AGCTGCTCCA GGTATCCCTT 
CCTGAAATTC AGGAACTTTA CCAGACTTTA CTTGCAAAGC CAAGCCCTGC TCAGCAGACT 
GACCGAAGCT CACCAGTGAG ACCCAGCAGT GAGAAGAATG ACTGTTGCCG AGGGAAGCGA 
GATGGAATTA ACAGTCTTGA GAGAAAACTG AAGAGACGCC TGGAAAGAGA GGGCCTCTCC 
AGTGAGCGGT GGGAACGAGT TAAGAAAATG CGGACCCCCA AAAAGAAGAA AATCAAACTG 
AGCCACCCCA AGGACATGAA CAATTTCAAG TTAGAGAGAG AGCGTAGCTA TGAATTAGTT 
CGTTCTGCTG AAACTCATTC CCTGCCCTCA GACACATCCT ATTCCGAACA GGAAGACTCT 
GAGGATGAAG ATGCCATCTG CCCAGCTGTG AGCTGCCTGC AGCCAGAAGG AGATGAGGTG 
GACTGGGTCC AGTGTGATGG CAGCTGCAAT CAGTGGTTTC ATCAGGTCTG TGTTGGTGTC 
TCCCCAGAGA TGGCAGAGAA AGAAGACTAC ATCTGTGTGC GCTGTACTGT GAAGGACGCA 
CCAAGCCGAA AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM5B ( NM_006618.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_006609.3 Homo sapiens lysine (K)-specific demethylase 5B (KDM5B), mRNA.

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CloneID	OHu27995
Clone ID Related Accession (Same CDS sequence)	NM_006618.4 , NM_006618.5 , NM_006618.3
Accession Version	NM_006618.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4635bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-14
Organism	Homo sapiens(Human)
Product	lysine-specific demethylase 5B
Comment

ATGGAGGCGG CCACCACACT GCACCCAGGC CCGCGCCCGG CGCTGCCCCT CGGGGGCCCG 
GGCCCGCTGG GCGAGTTCCT GCCTCCACCC GAGTGCCCGG TCTTCGAACC CAGCTGGGAA 
GAGTTCGCGG ACCCCTTCGC TTTCATCCAC AAGATCCGGC CCATAGCCGA GCAGACTGGC 
ATCTGTAAGG TGCGGCCGCC GCCGGATTGG CAGCCACCAT TTGCATGTGA TGTTGATAAA 
CTTCATTTTA CGCCACGTAT CCAGAGACTG AATGAATTGG AGGCCCAAAC TCGTGTAAAA 
TTGAATTTCT TGGACCAGAT TGCAAAGTAC TGGGAGTTAC AGGGAAGTAC TCTGAAAATT 
CCACATGTGG AGAGGAAGAT CTTGGACTTA TTTCAGCTTA ATAAGTTAGT TGCAGAAGAA 
GGTGGATTTG CAGTTGTTTG CAAGGATAGA AAATGGACCA AAATTGCTAC CAAGATGGGG 
TTTGCTCCTG GCAAAGCAGT GGGCTCACAT ATCAGAGGGC ATTATGAACG AATTCTCAAC 
CCCTACAACT TATTCCTGTC CGGAGACAGC CTAAGGTGTT TGCAGAAGCC AAACCTGACC 
ACAGACACTA AGGACAAGGA GTACAAACCC CATGATATTC CCCAGAGGCA GTCTGTGCAG 
CCTTCGGAAA CGTGCCCCCC AGCCCGACGA GCAAAACGCA TGAGAGCAGA GGCCATGAAT 
ATTAAAATAG AACCCGAGGA GACAACGGAA GCCAGAACTC ATAATCTGAG ACGTCGAATG 
GGTTGTCCAA CTCCAAAATG TGAAAATGAG AAAGAAATGA AGAGTAGCAT CAAGCAAGAA 
CCTATTGAGA GGAAAGATTA TATTGTAGAA AATGAGAAGG AAAAGCCCAA GAGTCGATCT 
AAAAAAGCCA CCAATGCTGT GGACCTGTAT GTCTGTCTTT TATGTGGCAG TGGCAATGAT 
GAAGACCGGC TACTGTTGTG TGATGGCTGT GATGACAGTT ACCATACCTT TTGCTTGATC 
CCACCTCTCC ATGATGTTCC CAAGGGAGAC TGGAGGTGTC CTAAGTGTTT GGCTCAGGAA 
TGTAGTAAGC CACAAGAAGC ATTTGGCTTT GAACAAGCAG CCAGGGACTA TACCCTCCGT 
ACTTTTGGGG AAATGGCAGA TGCGTTCAAA TCTGATTACT TCAACATGCC AGTCCATATG 
GTCCCCACAG AGCTTGTTGA GAAAGAATTT TGGAGACTAG TAAGCACTAT TGAGGAGGAT 
GTCACAGTGG AATATGGAGC TGACATTGCC TCAAAGGAAT TTGGCAGTGG CTTTCCTGTC 
CGAGATGGGA AAATCAAACT CTCACCTGAG GAAGAGGAGT ATCTTGATAG TGGCTGGAAT 
TTGAACAACA TGCCAGTGAT GGAGCAGTCT GTCCTTGCAC ATATTACTGC TGATATATGT 
GGCATGAAAC TTCCTTGGTT GTATGTGGGA ATGTGCTTTT CTTCATTCTG TTGGCACATT 
GAAGACCACT GGAGCTATTC AATTAACTAC TTGCACTGGG GTGAGCCAAA AACCTGGTAT 
GGAGTCCCAG GGTATGCTGC TGAGCAGCTA GAAAATGTAA TGAAGAAACT AGCTCCAGAA 
CTCTTTGTGT CCCAGCCGGA TCTCCTCCAT CAGCTTGTGA CCATCATGAA CCCCAATACC 
CTGATGACTC ATGAAGTGCC TGTTTACCGA ACTAATCAGT GTGCTGGGGA GTTTGTGATT 
ACATTTCCAA GAGCCTACCA CAGTGGTTTT AACCAGGGTT TTAATTTTGC TGAGGCTGTT 
AACTTCTGCA CTGTTGATTG GCTGCCATTA GGCCGACAGT GTGTGGAGCA TTATCGCTTG 
CTTCATCGAT ATTGTGTGTT TTCCCACGAT GAGATGATCT GCAAGATGGC TTCCAAGGCT 
GATGTATTAG ATGTTGTAGT GGCTTCAACT GTTCAGAAAG ACATGGCCAT TATGATTGAG 
GATGAGAAAG CTTTAAGAGA AACTGTCCGT AAATTGGGAG TGATTGATTC GGAAAGAATG 
GATTTTGAGC TGTTGCCAGA TGATGAACGT CAGTGTGTAA AATGCAAAAC TACATGCTTC 
ATGTCTGCCA TCTCCTGTTC TTGTAAACCT GGCCTTCTTG TTTGCCTGCA TCATGTAAAA 
GAATTGTGTT CCTGTCCTCC TTACAAATAT AAATTGCGGT ATAGGTACAC GCTGGATGAT 
CTCTACCCTA TGATGAATGC ATTGAAGCTT CGAGCAGAAT CTTACAACGA ATGGGCCTTG 
AATGTGAATG AAGCTTTGGA GGCAAAGATC AACAAGAAGA AAAGCCTTGT CAGCTTCAAG 
GCTTTAATTG AAGAATCTGA AATGAAGAAA TTCCCAGACA ATGATCTTTT GCGACACCTT 
CGCCTAGTCA CACAGGATGC AGAGAAGTGT GCCTCTGTTG CGCAGCAGTT GCTTAATGGC 
AAAAGGCAAA CTAGATATCG ATCTGGTGGA GGGAAATCCC AAAATCAGTT GACAGTGAAT 
GAGCTCCGGC AGTTTGTAAC ACAGCTGTAT GCTCTTCCAT GTGTCCTCAG TCAGACACCA 
TTACTAAAGG ATCTCTTGAA TCGTGTAGAA GATTTTCAAC AGCATAGTCA GAAACTACTC 
TCTGAGGAAA CGCCTAGTGC TGCGGAGCTG CAGGACTTGC TAGATGTCAG CTTTGAATTT 
GATGTTGAAC TTCCACAGCT TGCTGAGATG CGTATCCGTT TGGAACAAGC CCGTTGGCTA 
GAAGAGGTGC AGCAAGCTTG CCTAGACCCC AGCTCCCTTA CTTTAGATGA TATGAGACGT 
CTCATAGACC TAGGGGTAGG GCTGGCCCCG TATTCAGCAG TGGAGAAAGC TATGGCCCGG 
CTGCAGGAAC TGCTCACAGT GTCAGAGCAC TGGGACGACA AAGCCAAGAG TCTCCTCAAG 
GCCAGGCCAC GACATTCATT GAATAGCCTT GCTACGGCAG TAAAGGAAAT CGAAGAGATC 
CCTGCATATC TGCCCAATGG TGCGGCTCTG AAAGACTCAG TGCAGAGAGC CAGAGACTGG 
CTTCAGGATG TAGAGGGCCT GCAGGCTGGA GGACGTGTGC CAGTGTTAGA CACACTCATA 
GAACTTGTTA CACGAGGCCG ATCTATCCCC GTACATCTGA ATTCTTTGCC AAGACTGGAA 
ACCCTAGTAG CTGAGGTTCA GGCTTGGAAA GAATGTGCTG TTAATACATT CTTGACTGAG 
AATTCTCCAT ATTCTCTCTT AGAGGTGCTG TGTCCTCGAT GTGATATTGG CCTTTTGGGA 
TTGAAAAGGA AGCAGAGAAA GTTAAAGGAG CCCTTGCCAA ATGGAAAGAA AAAAAGCACC 
AAATTAGAGA GTCTGAGTGA CCTGGAGAGA GCTTTAACTG AAAGCAAGGA GACTGCTTCA 
GCTATGGCAA CTCTTGGGGA AGCTCGCCTA AGGGAAATGG AAGCCTTGCA GTCTCTCAGA 
CTCGCCAATG AAGGGAAATT GCTGTCGCCT CTCCAAGATG TGGATATAAA AATCTGCCTA 
TGTCAGAAGG CCCCAGCTGC CCCTATGATT CAATGTGAAC TCTGCAGGGA TGCTTTCCAC 
ACCAGTTGTG TGGCGGTACC CAGTATTTCA CAGGGCCTGC GAATCTGGCT TTGTCCCCAT 
TGTCGGAGGT CAGAGAAACC TCCATTAGAG AAAATTCTGC CCCTGCTCGC CTCCCTTCAG 
CGTATCCGAG TTCGCCTTCC TGAGGGAGAT GCACTTCGAT ATATGATTGA AAGAACCGTG 
AACTGGCAGC ACAGAGCCCA GCAACTGCTT TCGTCAGGGA ATCTTAAATT TGTGCAAGAT 
CGAGTGGGCT CAGGACTGTT ATATAGCAGA TGGCAAGCCT CAGCAGGACA GGTGTCAGAC 
ACAAACAAGG TATCTCAACC TCCTGGCACA ACATCATTTT CTTTGCCTGA TGACTGGGAC 
AACAGAACCT CATATTTGCA CTCCCCCTTC TCAACTGGAC GAAGTTGTAT CCCCCTCCAT 
GGTGTTAGTC CAGAAGTGAA TGAACTATTG ATGGAAGCCC AGCTGCTCCA GGTATCCCTT 
CCTGAAATTC AGGAACTTTA CCAGACTTTA CTTGCAAAGC CAAGCCCTGC TCAGCAGACT 
GACCGAAGCT CACCAGTGAG ACCCAGCAGT GAGAAGAATG ACTGTTGCCG AGGGAAGCGA 
GATGGAATTA ACAGTCTTGA GAGAAAACTG AAGAGACGCC TGGAAAGAGA GGGCCTCTCC 
AGTGAGCGGT GGGAACGAGT TAAGAAAATG CGGACCCCCA AAAAGAAGAA AATCAAACTG 
AGCCACCCCA AGGACATGAA CAATTTCAAG TTAGAGAGAG AGCGTAGCTA TGAATTAGTT 
CGTTCTGCTG AAACTCATTC CCTGCCCTCA GACACATCCT ATTCCGAACA GGAAGACTCT 
GAGGATGAAG ATGCCATCTG CCCAGCTGTG AGCTGCCTGC AGCCAGAAGG AGATGAGGTG 
GACTGGGTCC AGTGTGATGG CAGCTGCAAT CAGTGGTTTC ATCAGGTCTG TGTTGGTGTC 
TCCCCAGAGA TGGCAGAGAA AGAAGACTAC ATCTGTGTGC GCTGTACTGT GAAGGACGCA 
CCAAGCCGAA AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

NP_006609.3

Translation

MEAATTLHPGPRPALPLGGPGPLGEFLPPPECPVFEPSWEEFADPFAFIHKIRPIAEQTGICKVRPPPDWQPPFACDVDKLHFTPRIQRLNELEAQTRVKLNFLDQIAKYWELQGSTLKIPHVERKILDLFQLNKLVAEEGGFAVVCKDRKWTKIATKMGFAPGKAVGSHIRGHYERILNPYNLFLSGDSLRCLQKPNLTTDTKDKEYKPHDIPQRQSVQPSETCPPARRAKRMRAEAMNIKIEPEETTEARTHNLRRRMGCPTPKCENEKEMKSSIKQEPIERKDYIVENEKEKPKSRSKKATNAVDLYVCLLCGSGNDEDRLLLCDGCDDSYHTFCLIPPLHDVPKGDWRCPKCLAQECSKPQEAFGFEQAARDYTLRTFGEMADAFKSDYFNMPVHMVPTELVEKEFWRLVSTIEEDVTVEYGADIASKEFGSGFPVRDGKIKLSPEEEEYLDSGWNLNNMPVMEQSVLAHITADICGMKLPWLYVGMCFSSFCWHIEDHWSYSINYLHWGEPKTWYGVPGYAAEQLENVMKKLAPELFVSQPDLLHQLVTIMNPNTLMTHEVPVYRTNQCAGEFVITFPRAYHSGFNQGFNFAEAVNFCTVDWLPLGRQCVEHYRLLHRYCVFSHDEMICKMASKADVLDVVVASTVQKDMAIMIEDEKALRETVRKLGVIDSERMDFELLPDDERQCVKCKTTCFMSAISCSCKPGLLVCLHHVKELCSCPPYKYKLRYRYTLDDLYPMMNALKLRAESYNEWALNVNEALEAKINKKKSLVSFKALIEESEMKKFPDNDLLRHLRLVTQDAEKCASVAQQLLNGKRQTRYRSGGGKSQNQLTVNELRQFVTQLYALPCVLSQTPLLKDLLNRVEDFQQHSQKLLSEETPSAAELQDLLDVSFEFDVELPQLAEMRIRLEQARWLEEVQQACLDPSSLTLDDMRRLIDLGVGLAPYSAVEKAMARLQELLTVSEHWDDKAKSLLKARPRHSLNSLATAVKEIEEIPAYLPNGAALKDSVQRARDWLQDVEGLQAGGRVPVLDTLIELVTRGRSIPVHLNSLPRLETLVAEVQAWKECAVNTFLTENSPYSLLEVLCPRCDIGLLGLKRKQRKLKEPLPNGKKKSTKLESLSDLERALTESKETASAMATLGEARLREMEALQSLRLANEGKLLSPLQDVDIKICLCQKAPAAPMIQCELCRDAFHTSCVAVPSISQGLRIWLCPHCRRSEKPPLEKILPLLASLQRIRVRLPEGDALRYMIERTVNWQHRAQQLLSSGNLKFVQDRVGSGLLYSRWQASAGQVSDTNKVSQPPGTTSFSLPDDWDNRTSYLHSPFSTGRSCIPLHGVSPEVNELLMEAQLLQVSLPEIQELYQTLLAKPSPAQQTDRSSPVRPSSEKNDCCRGKRDGINSLERKLKRRLEREGLSSERWERVKKMRTPKKKKIKLSHPKDMNNFKLERERSYELVRSAETHSLPSDTSYSEQEDSEDEDAICPAVSCLQPEGDEVDWVQCDGSCNQWFHQVCVGVSPEMAEKEDYICVRCTVKDAPSRK

Target ORF information:

RefSeq Version	NM_006618.3
Organism	Homo sapiens(Human)
Definition	Homo sapiens lysine (K)-specific demethylase 5B (KDM5B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006618.3

ORF Insert Sequence:

ATGGAGGCGG CCACCACACT GCACCCAGGC CCGCGCCCGG CGCTGCCCCT CGGGGGCCCG 
GGCCCGCTGG GCGAGTTCCT GCCTCCACCC GAGTGCCCGG TCTTCGAACC CAGCTGGGAA 
GAGTTCGCGG ACCCCTTCGC TTTCATCCAC AAGATCCGGC CCATAGCCGA GCAGACTGGC 
ATCTGTAAGG TGCGGCCGCC GCCGGATTGG CAGCCACCAT TTGCATGTGA TGTTGATAAA 
CTTCATTTTA CGCCACGTAT CCAGAGACTG AATGAATTGG AGGCCCAAAC TCGTGTAAAA 
TTGAATTTCT TGGACCAGAT TGCAAAGTAC TGGGAGTTAC AGGGAAGTAC TCTGAAAATT 
CCACATGTGG AGAGGAAGAT CTTGGACTTA TTTCAGCTTA ATAAGTTAGT TGCAGAAGAA 
GGTGGATTTG CAGTTGTTTG CAAGGATAGA AAATGGACCA AAATTGCTAC CAAGATGGGG 
TTTGCTCCTG GCAAAGCAGT GGGCTCACAT ATCAGAGGGC ATTATGAACG AATTCTCAAC 
CCCTACAACT TATTCCTGTC CGGAGACAGC CTAAGGTGTT TGCAGAAGCC AAACCTGACC 
ACAGACACTA AGGACAAGGA GTACAAACCC CATGATATTC CCCAGAGGCA GTCTGTGCAG 
CCTTCGGAAA CGTGCCCCCC AGCCCGACGA GCAAAACGCA TGAGAGCAGA GGCCATGAAT 
ATTAAAATAG AACCCGAGGA GACAACGGAA GCCAGAACTC ATAATCTGAG ACGTCGAATG 
GGTTGTCCAA CTCCAAAATG TGAAAATGAG AAAGAAATGA AGAGTAGCAT CAAGCAAGAA 
CCTATTGAGA GGAAAGATTA TATTGTAGAA AATGAGAAGG AAAAGCCCAA GAGTCGATCT 
AAAAAAGCCA CCAATGCTGT GGACCTGTAT GTCTGTCTTT TATGTGGCAG TGGCAATGAT 
GAAGACCGGC TACTGTTGTG TGATGGCTGT GATGACAGTT ACCATACCTT TTGCTTGATC 
CCACCTCTCC ATGATGTTCC CAAGGGAGAC TGGAGGTGTC CTAAGTGTTT GGCTCAGGAA 
TGTAGTAAGC CACAAGAAGC ATTTGGCTTT GAACAAGCAG CCAGGGACTA TACCCTCCGT 
ACTTTTGGGG AAATGGCAGA TGCGTTCAAA TCTGATTACT TCAACATGCC AGTCCATATG 
GTCCCCACAG AGCTTGTTGA GAAAGAATTT TGGAGACTAG TAAGCACTAT TGAGGAGGAT 
GTCACAGTGG AATATGGAGC TGACATTGCC TCAAAGGAAT TTGGCAGTGG CTTTCCTGTC 
CGAGATGGGA AAATCAAACT CTCACCTGAG GAAGAGGAGT ATCTTGATAG TGGCTGGAAT 
TTGAACAACA TGCCAGTGAT GGAGCAGTCT GTCCTTGCAC ATATTACTGC TGATATATGT 
GGCATGAAAC TTCCTTGGTT GTATGTGGGA ATGTGCTTTT CTTCATTCTG TTGGCACATT 
GAAGACCACT GGAGCTATTC AATTAACTAC TTGCACTGGG GTGAGCCAAA AACCTGGTAT 
GGAGTCCCAG GGTATGCTGC TGAGCAGCTA GAAAATGTAA TGAAGAAACT AGCTCCAGAA 
CTCTTTGTGT CCCAGCCGGA TCTCCTCCAT CAGCTTGTGA CCATCATGAA CCCCAATACC 
CTGATGACTC ATGAAGTGCC TGTTTACCGA ACTAATCAGT GTGCTGGGGA GTTTGTGATT 
ACATTTCCAA GAGCCTACCA CAGTGGTTTT AACCAGGGTT TTAATTTTGC TGAGGCTGTT 
AACTTCTGCA CTGTTGATTG GCTGCCATTA GGCCGACAGT GTGTGGAGCA TTATCGCTTG 
CTTCATCGAT ATTGTGTGTT TTCCCACGAT GAGATGATCT GCAAGATGGC TTCCAAGGCT 
GATGTATTAG ATGTTGTAGT GGCTTCAACT GTTCAGAAAG ACATGGCCAT TATGATTGAG 
GATGAGAAAG CTTTAAGAGA AACTGTCCGT AAATTGGGAG TGATTGATTC GGAAAGAATG 
GATTTTGAGC TGTTGCCAGA TGATGAACGT CAGTGTGTAA AATGCAAAAC TACATGCTTC 
ATGTCTGCCA TCTCCTGTTC TTGTAAACCT GGCCTTCTTG TTTGCCTGCA TCATGTAAAA 
GAATTGTGTT CCTGTCCTCC TTACAAATAT AAATTGCGGT ATAGGTACAC GCTGGATGAT 
CTCTACCCTA TGATGAATGC ATTGAAGCTT CGAGCAGAAT CTTACAACGA ATGGGCCTTG 
AATGTGAATG AAGCTTTGGA GGCAAAGATC AACAAGAAGA AAAGCCTTGT CAGCTTCAAG 
GCTTTAATTG AAGAATCTGA AATGAAGAAA TTCCCAGACA ATGATCTTTT GCGACACCTT 
CGCCTAGTCA CACAGGATGC AGAGAAGTGT GCCTCTGTTG CGCAGCAGTT GCTTAATGGC 
AAAAGGCAAA CTAGATATCG ATCTGGTGGA GGGAAATCCC AAAATCAGTT GACAGTGAAT 
GAGCTCCGGC AGTTTGTAAC ACAGCTGTAT GCTCTTCCAT GTGTCCTCAG TCAGACACCA 
TTACTAAAGG ATCTCTTGAA TCGTGTAGAA GATTTTCAAC AGCATAGTCA GAAACTACTC 
TCTGAGGAAA CGCCTAGTGC TGCGGAGCTG CAGGACTTGC TAGATGTCAG CTTTGAATTT 
GATGTTGAAC TTCCACAGCT TGCTGAGATG CGTATCCGTT TGGAACAAGC CCGTTGGCTA 
GAAGAGGTGC AGCAAGCTTG CCTAGACCCC AGCTCCCTTA CTTTAGATGA TATGAGACGT 
CTCATAGACC TAGGGGTAGG GCTGGCCCCG TATTCAGCAG TGGAGAAAGC TATGGCCCGG 
CTGCAGGAAC TGCTCACAGT GTCAGAGCAC TGGGACGACA AAGCCAAGAG TCTCCTCAAG 
GCCAGGCCAC GACATTCATT GAATAGCCTT GCTACGGCAG TAAAGGAAAT CGAAGAGATC 
CCTGCATATC TGCCCAATGG TGCGGCTCTG AAAGACTCAG TGCAGAGAGC CAGAGACTGG 
CTTCAGGATG TAGAGGGCCT GCAGGCTGGA GGACGTGTGC CAGTGTTAGA CACACTCATA 
GAACTTGTTA CACGAGGCCG ATCTATCCCC GTACATCTGA ATTCTTTGCC AAGACTGGAA 
ACCCTAGTAG CTGAGGTTCA GGCTTGGAAA GAATGTGCTG TTAATACATT CTTGACTGAG 
AATTCTCCAT ATTCTCTCTT AGAGGTGCTG TGTCCTCGAT GTGATATTGG CCTTTTGGGA 
TTGAAAAGGA AGCAGAGAAA GTTAAAGGAG CCCTTGCCAA ATGGAAAGAA AAAAAGCACC 
AAATTAGAGA GTCTGAGTGA CCTGGAGAGA GCTTTAACTG AAAGCAAGGA GACTGCTTCA 
GCTATGGCAA CTCTTGGGGA AGCTCGCCTA AGGGAAATGG AAGCCTTGCA GTCTCTCAGA 
CTCGCCAATG AAGGGAAATT GCTGTCGCCT CTCCAAGATG TGGATATAAA AATCTGCCTA 
TGTCAGAAGG CCCCAGCTGC CCCTATGATT CAATGTGAAC TCTGCAGGGA TGCTTTCCAC 
ACCAGTTGTG TGGCGGTACC CAGTATTTCA CAGGGCCTGC GAATCTGGCT TTGTCCCCAT 
TGTCGGAGGT CAGAGAAACC TCCATTAGAG AAAATTCTGC CCCTGCTCGC CTCCCTTCAG 
CGTATCCGAG TTCGCCTTCC TGAGGGAGAT GCACTTCGAT ATATGATTGA AAGAACCGTG 
AACTGGCAGC ACAGAGCCCA GCAACTGCTT TCGTCAGGGA ATCTTAAATT TGTGCAAGAT 
CGAGTGGGCT CAGGACTGTT ATATAGCAGA TGGCAAGCCT CAGCAGGACA GGTGTCAGAC 
ACAAACAAGG TATCTCAACC TCCTGGCACA ACATCATTTT CTTTGCCTGA TGACTGGGAC 
AACAGAACCT CATATTTGCA CTCCCCCTTC TCAACTGGAC GAAGTTGTAT CCCCCTCCAT 
GGTGTTAGTC CAGAAGTGAA TGAACTATTG ATGGAAGCCC AGCTGCTCCA GGTATCCCTT 
CCTGAAATTC AGGAACTTTA CCAGACTTTA CTTGCAAAGC CAAGCCCTGC TCAGCAGACT 
GACCGAAGCT CACCAGTGAG ACCCAGCAGT GAGAAGAATG ACTGTTGCCG AGGGAAGCGA 
GATGGAATTA ACAGTCTTGA GAGAAAACTG AAGAGACGCC TGGAAAGAGA GGGCCTCTCC 
AGTGAGCGGT GGGAACGAGT TAAGAAAATG CGGACCCCCA AAAAGAAGAA AATCAAACTG 
AGCCACCCCA AGGACATGAA CAATTTCAAG TTAGAGAGAG AGCGTAGCTA TGAATTAGTT 
CGTTCTGCTG AAACTCATTC CCTGCCCTCA GACACATCCT ATTCCGAACA GGAAGACTCT 
GAGGATGAAG ATGCCATCTG CCCAGCTGTG AGCTGCCTGC AGCCAGAAGG AGATGAGGTG 
GACTGGGTCC AGTGTGATGG CAGCTGCAAT CAGTGGTTTC ATCAGGTCTG TGTTGGTGTC 
TCCCCAGAGA TGGCAGAGAA AGAAGACTAC ATCTGTGTGC GCTGTACTGT GAAGGACGCA 
CCAAGCCGAA AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM5B ( NM_001314042.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001300971.1 Homo sapiens lysine demethylase 5B (KDM5B), transcript variant 1, mRNA.

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CloneID	OHu64356
Clone ID Related Accession (Same CDS sequence)	NM_001314042.1
Accession Version	NM_001314042.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4743bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-09
Organism	Homo sapiens(human)
Product	lysine-specific demethylase 5B isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC098934.3, AF087481.1, AC104463.3 and AI262935.1. This sequence is a reference standard in the RefSeqGene project. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.14373.1, AF087481.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: upstream AUG and CDS extension is not conserved ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGAGGCGG CCACCACACT GCACCCAGGC CCGCGCCCGG CGCTGCCCCT CGGGGGCCCG 
GGCCCGCTGG GCGAGTTCCT GCCTCCACCC GAGTGCCCGG TCTTCGAACC CAGCTGGGAA 
GAGTTCGCGG ACCCCTTCGC TTTCATCCAC AAGATCCGGC CCATAGCCGA GCAGACTGGC 
ATCTGTAAGG TGCGGCCGCC GCCGGATTGG CAGCCACCAT TTGCATGTGA TGTTGATAAA 
CTTCATTTTA CGCCACGTAT CCAGAGACTG AATGAATTGG AGGCCCAAAC TCGTGTAAAA 
TTGAATTTCT TGGACCAGAT TGCAAAGTAC TGGGAGTTAC AGGGAAGTAC TCTGAAAATT 
CCACATGTGG AGAGGAAGAT CTTGGACTTA TTTCAGCTTA ATAAGTTAGT TGCAGAAGAA 
GGTGGATTTG CAGTTGTTTG CAAGGATAGA AAATGGACCA AAATTGCTAC CAAGATGGGG 
TTTGCTCCTG GCAAAGCAGT GGGCTCACAT ATCAGAGGGC ATTATGAACG AATTCTCAAC 
CCCTACAACT TATTCCTGTC CGGAGACAGC CTAAGGTGTT TGCAGAAGCC AAACCTGACC 
ACAGACACTA AGGACAAGGA GTACAAACCC CATGATATTC CCCAGAGGCA GTCTGTGCAG 
CCTTCGGAAA CGTGCCCCCC AGCCCGACGA GCAAAACGCA TGAGAGCAGA GAGACAGAGT 
CTCGCTGTGT TGCCCAGGCT GGAGTGCAGT GGCGCGATCT TGGCTCACTG CAACCTCCGC 
CTCCTAGATT CCAGCAATTC TTCTGCCTCA GCCTCCCAAG CCATGAATAT TAAAATAGAA 
CCCGAGGAGA CAACGGAAGC CAGAACTCAT AATCTGAGAC GTCGAATGGG TTGTCCAACT 
CCAAAATGTG AAAATGAGAA AGAAATGAAG AGTAGCATCA AGCAAGAACC TATTGAGAGG 
AAAGATTATA TTGTAGAAAA TGAGAAGGAA AAGCCCAAGA GTCGATCTAA AAAAGCCACC 
AATGCTGTGG ACCTGTATGT CTGTCTTTTA TGTGGCAGTG GCAATGATGA AGACCGGCTA 
CTGTTGTGTG ATGGCTGTGA TGACAGTTAC CATACCTTTT GCTTGATCCC ACCTCTCCAT 
GATGTTCCCA AGGGAGACTG GAGGTGTCCT AAGTGTTTGG CTCAGGAATG TAGTAAGCCA 
CAAGAAGCAT TTGGCTTTGA ACAAGCAGCC AGGGACTATA CCCTCCGTAC TTTTGGGGAA 
ATGGCAGATG CGTTCAAATC TGATTACTTC AACATGCCAG TCCATATGGT CCCCACAGAG 
CTTGTTGAGA AAGAATTTTG GAGACTAGTA AGCACTATTG AGGAGGATGT CACAGTGGAA 
TATGGAGCTG ACATTGCCTC AAAGGAATTT GGCAGTGGCT TTCCTGTCCG AGATGGGAAA 
ATCAAACTCT CACCTGAGGA AGAGGAGTAT CTTGATAGTG GCTGGAATTT GAACAACATG 
CCAGTGATGG AGCAGTCTGT CCTTGCACAT ATTACTGCTG ATATATGTGG CATGAAACTT 
CCTTGGTTGT ATGTGGGAAT GTGCTTTTCT TCATTCTGTT GGCACATTGA AGACCACTGG 
AGCTATTCAA TTAACTACTT GCACTGGGGT GAGCCAAAAA CCTGGTATGG AGTCCCAGGG 
TATGCTGCTG AGCAGCTAGA AAATGTAATG AAGAAACTAG CTCCAGAACT CTTTGTGTCC 
CAGCCGGATC TCCTCCATCA GCTTGTGACC ATCATGAACC CCAATACCCT GATGACTCAT 
GAAGTGCCTG TTTACCGAAC TAATCAGTGT GCTGGGGAGT TTGTGATTAC ATTTCCAAGA 
GCCTACCACA GTGGTTTTAA CCAGGGTTTT AATTTTGCTG AGGCTGTTAA CTTCTGCACT 
GTTGATTGGC TGCCATTAGG CCGACAGTGT GTGGAGCATT ATCGCTTGCT TCATCGATAT 
TGTGTGTTTT CCCACGATGA GATGATCTGC AAGATGGCTT CCAAGGCTGA TGTATTAGAT 
GTTGTAGTGG CTTCAACTGT TCAGAAAGAC ATGGCCATTA TGATTGAGGA TGAGAAAGCT 
TTAAGAGAAA CTGTCCGTAA ATTGGGAGTG ATTGATTCGG AAAGAATGGA TTTTGAGCTG 
TTGCCAGATG ATGAACGTCA GTGTGTAAAA TGCAAAACTA CATGCTTCAT GTCTGCCATC 
TCCTGTTCTT GTAAACCTGG CCTTCTTGTT TGCCTGCATC ATGTAAAAGA ATTGTGTTCC 
TGTCCTCCTT ACAAATATAA ATTGCGGTAT AGGTACACGC TGGATGATCT CTACCCTATG 
ATGAATGCAT TGAAGCTTCG AGCAGAATCT TACAACGAAT GGGCCTTGAA TGTGAATGAA 
GCTTTGGAGG CAAAGATCAA CAAGAAGAAA AGCCTTGTCA GCTTCAAGGC TTTAATTGAA 
GAATCTGAAA TGAAGAAATT CCCAGACAAT GATCTTTTGC GACACCTTCG CCTAGTCACA 
CAGGATGCAG AGAAGTGTGC CTCTGTTGCG CAGCAGTTGC TTAATGGCAA AAGGCAAACT 
AGATATCGAT CTGGTGGAGG GAAATCCCAA AATCAGTTGA CAGTGAATGA GCTCCGGCAG 
TTTGTAACAC AGCTGTATGC TCTTCCATGT GTCCTCAGTC AGACACCATT ACTAAAGGAT 
CTCTTGAATC GTGTAGAAGA TTTTCAACAG CATAGTCAGA AACTACTCTC TGAGGAAACG 
CCTAGTGCTG CGGAGCTGCA GGACTTGCTA GATGTCAGCT TTGAATTTGA TGTTGAACTT 
CCACAGCTTG CTGAGATGCG TATCCGTTTG GAACAAGCCC GTTGGCTAGA AGAGGTGCAG 
CAAGCTTGCC TAGACCCCAG CTCCCTTACT TTAGATGATA TGAGACGTCT CATAGACCTA 
GGGGTAGGGC TGGCCCCGTA TTCAGCAGTG GAGAAAGCTA TGGCCCGGCT GCAGGAACTG 
CTCACAGTGT CAGAGCACTG GGACGACAAA GCCAAGAGTC TCCTCAAGGC CAGGCCACGA 
CATTCATTGA ATAGCCTTGC TACGGCAGTA AAGGAAATCG AAGAGATCCC TGCATATCTG 
CCCAATGGTG CGGCTCTGAA AGACTCAGTG CAGAGAGCCA GAGACTGGCT TCAGGATGTA 
GAGGGCCTGC AGGCTGGAGG ACGTGTGCCA GTGTTAGACA CACTCATAGA ACTTGTTACA 
CGAGGCCGAT CTATCCCCGT ACATCTGAAT TCTTTGCCAA GACTGGAAAC CCTAGTAGCT 
GAGGTTCAGG CTTGGAAAGA ATGTGCTGTT AATACATTCT TGACTGAGAA TTCTCCATAT 
TCTCTCTTAG AGGTGCTGTG TCCTCGATGT GATATTGGCC TTTTGGGATT GAAAAGGAAG 
CAGAGAAAGT TAAAGGAGCC CTTGCCAAAT GGAAAGAAAA AAAGCACCAA ATTAGAGAGT 
CTGAGTGACC TGGAGAGAGC TTTAACTGAA AGCAAGGAGA CTGCTTCAGC TATGGCAACT 
CTTGGGGAAG CTCGCCTAAG GGAAATGGAA GCCTTGCAGT CTCTCAGACT CGCCAATGAA 
GGGAAATTGC TGTCGCCTCT CCAAGATGTG GATATAAAAA TCTGCCTATG TCAGAAGGCC 
CCAGCTGCCC CTATGATTCA ATGTGAACTC TGCAGGGATG CTTTCCACAC CAGTTGTGTG 
GCGGTACCCA GTATTTCACA GGGCCTGCGA ATCTGGCTTT GTCCCCATTG TCGGAGGTCA 
GAGAAACCTC CATTAGAGAA AATTCTGCCC CTGCTCGCCT CCCTTCAGCG TATCCGAGTT 
CGCCTTCCTG AGGGAGATGC ACTTCGATAT ATGATTGAAA GAACCGTGAA CTGGCAGCAC 
AGAGCCCAGC AACTGCTTTC GTCAGGGAAT CTTAAATTTG TGCAAGATCG AGTGGGCTCA 
GGACTGTTAT ATAGCAGATG GCAAGCCTCA GCAGGACAGG TGTCAGACAC AAACAAGGTA 
TCTCAACCTC CTGGCACAAC ATCATTTTCT TTGCCTGATG ACTGGGACAA CAGAACCTCA 
TATTTGCACT CCCCCTTCTC AACTGGACGA AGTTGTATCC CCCTCCATGG TGTTAGTCCA 
GAAGTGAATG AACTATTGAT GGAAGCCCAG CTGCTCCAGG TATCCCTTCC TGAAATTCAG 
GAACTTTACC AGACTTTACT TGCAAAGCCA AGCCCTGCTC AGCAGACTGA CCGAAGCTCA 
CCAGTGAGAC CCAGCAGTGA GAAGAATGAC TGTTGCCGAG GGAAGCGAGA TGGAATTAAC 
AGTCTTGAGA GAAAACTGAA GAGACGCCTG GAAAGAGAGG GCCTCTCCAG TGAGCGGTGG 
GAACGAGTTA AGAAAATGCG GACCCCCAAA AAGAAGAAAA TCAAACTGAG CCACCCCAAG 
GACATGAACA ATTTCAAGTT AGAGAGAGAG CGTAGCTATG AATTAGTTCG TTCTGCTGAA 
ACTCATTCCC TGCCCTCAGA CACATCCTAT TCCGAACAGG AAGACTCTGA GGATGAAGAT 
GCCATCTGCC CAGCTGTGAG CTGCCTGCAG CCAGAAGGAG ATGAGGTGGA CTGGGTCCAG 
TGTGATGGCA GCTGCAATCA GTGGTTTCAT CAGGTCTGTG TTGGTGTCTC CCCAGAGATG 
GCAGAGAAAG AAGACTACAT CTGTGTGCGC TGTACTGTGA AGGACGCACC AAGCCGAAAG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001300971.1
CDS	1166..5908
Translation	MEAATTLHPGPRPALPLGGPGPLGEFLPPPECPVFEPSWEEFADPFAFIHKIRPIAEQTGICKVRPPPDWQPPFACDVDKLHFTPRIQRLNELEAQTRVKLNFLDQIAKYWELQGSTLKIPHVERKILDLFQLNKLVAEEGGFAVVCKDRKWTKIATKMGFAPGKAVGSHIRGHYERILNPYNLFLSGDSLRCLQKPNLTTDTKDKEYKPHDIPQRQSVQPSETCPPARRAKRMRAERQSLAVLPRLECSGAILAHCNLRLLDSSNSSASASQAMNIKIEPEETTEARTHNLRRRMGCPTPKCENEKEMKSSIKQEPIERKDYIVENEKEKPKSRSKKATNAVDLYVCLLCGSGNDEDRLLLCDGCDDSYHTFCLIPPLHDVPKGDWRCPKCLAQECSKPQEAFGFEQAARDYTLRTFGEMADAFKSDYFNMPVHMVPTELVEKEFWRLVSTIEEDVTVEYGADIASKEFGSGFPVRDGKIKLSPEEEEYLDSGWNLNNMPVMEQSVLAHITADICGMKLPWLYVGMCFSSFCWHIEDHWSYSINYLHWGEPKTWYGVPGYAAEQLENVMKKLAPELFVSQPDLLHQLVTIMNPNTLMTHEVPVYRTNQCAGEFVITFPRAYHSGFNQGFNFAEAVNFCTVDWLPLGRQCVEHYRLLHRYCVFSHDEMICKMASKADVLDVVVASTVQKDMAIMIEDEKALRETVRKLGVIDSERMDFELLPDDERQCVKCKTTCFMSAISCSCKPGLLVCLHHVKELCSCPPYKYKLRYRYTLDDLYPMMNALKLRAESYNEWALNVNEALEAKINKKKSLVSFKALIEESEMKKFPDNDLLRHLRLVTQDAEKCASVAQQLLNGKRQTRYRSGGGKSQNQLTVNELRQFVTQLYALPCVLSQTPLLKDLLNRVEDFQQHSQKLLSEETPSAAELQDLLDVSFEFDVELPQLAEMRIRLEQARWLEEVQQACLDPSSLTLDDMRRLIDLGVGLAPYSAVEKAMARLQELLTVSEHWDDKAKSLLKARPRHSLNSLATAVKEIEEIPAYLPNGAALKDSVQRARDWLQDVEGLQAGGRVPVLDTLIELVTRGRSIPVHLNSLPRLETLVAEVQAWKECAVNTFLTENSPYSLLEVLCPRCDIGLLGLKRKQRKLKEPLPNGKKKSTKLESLSDLERALTESKETASAMATLGEARLREMEALQSLRLANEGKLLSPLQDVDIKICLCQKAPAAPMIQCELCRDAFHTSCVAVPSISQGLRIWLCPHCRRSEKPPLEKILPLLASLQRIRVRLPEGDALRYMIERTVNWQHRAQQLLSSGNLKFVQDRVGSGLLYSRWQASAGQVSDTNKVSQPPGTTSFSLPDDWDNRTSYLHSPFSTGRSCIPLHGVSPEVNELLMEAQLLQVSLPEIQELYQTLLAKPSPAQQTDRSSPVRPSSEKNDCCRGKRDGINSLERKLKRRLEREGLSSERWERVKKMRTPKKKKIKLSHPKDMNNFKLERERSYELVRSAETHSLPSDTSYSEQEDSEDEDAICPAVSCLQPEGDEVDWVQCDGSCNQWFHQVCVGVSPEMAEKEDYICVRCTVKDAPSRK

Target ORF information:

RefSeq Version	NM_001314042.1
Organism	Homo sapiens(human)
Definition	Homo sapiens lysine demethylase 5B (KDM5B), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001314042.1

ORF Insert Sequence:

ATGGAGGCGG CCACCACACT GCACCCAGGC CCGCGCCCGG CGCTGCCCCT CGGGGGCCCG 
GGCCCGCTGG GCGAGTTCCT GCCTCCACCC GAGTGCCCGG TCTTCGAACC CAGCTGGGAA 
GAGTTCGCGG ACCCCTTCGC TTTCATCCAC AAGATCCGGC CCATAGCCGA GCAGACTGGC 
ATCTGTAAGG TGCGGCCGCC GCCGGATTGG CAGCCACCAT TTGCATGTGA TGTTGATAAA 
CTTCATTTTA CGCCACGTAT CCAGAGACTG AATGAATTGG AGGCCCAAAC TCGTGTAAAA 
TTGAATTTCT TGGACCAGAT TGCAAAGTAC TGGGAGTTAC AGGGAAGTAC TCTGAAAATT 
CCACATGTGG AGAGGAAGAT CTTGGACTTA TTTCAGCTTA ATAAGTTAGT TGCAGAAGAA 
GGTGGATTTG CAGTTGTTTG CAAGGATAGA AAATGGACCA AAATTGCTAC CAAGATGGGG 
TTTGCTCCTG GCAAAGCAGT GGGCTCACAT ATCAGAGGGC ATTATGAACG AATTCTCAAC 
CCCTACAACT TATTCCTGTC CGGAGACAGC CTAAGGTGTT TGCAGAAGCC AAACCTGACC 
ACAGACACTA AGGACAAGGA GTACAAACCC CATGATATTC CCCAGAGGCA GTCTGTGCAG 
CCTTCGGAAA CGTGCCCCCC AGCCCGACGA GCAAAACGCA TGAGAGCAGA GAGACAGAGT 
CTCGCTGTGT TGCCCAGGCT GGAGTGCAGT GGCGCGATCT TGGCTCACTG CAACCTCCGC 
CTCCTAGATT CCAGCAATTC TTCTGCCTCA GCCTCCCAAG CCATGAATAT TAAAATAGAA 
CCCGAGGAGA CAACGGAAGC CAGAACTCAT AATCTGAGAC GTCGAATGGG TTGTCCAACT 
CCAAAATGTG AAAATGAGAA AGAAATGAAG AGTAGCATCA AGCAAGAACC TATTGAGAGG 
AAAGATTATA TTGTAGAAAA TGAGAAGGAA AAGCCCAAGA GTCGATCTAA AAAAGCCACC 
AATGCTGTGG ACCTGTATGT CTGTCTTTTA TGTGGCAGTG GCAATGATGA AGACCGGCTA 
CTGTTGTGTG ATGGCTGTGA TGACAGTTAC CATACCTTTT GCTTGATCCC ACCTCTCCAT 
GATGTTCCCA AGGGAGACTG GAGGTGTCCT AAGTGTTTGG CTCAGGAATG TAGTAAGCCA 
CAAGAAGCAT TTGGCTTTGA ACAAGCAGCC AGGGACTATA CCCTCCGTAC TTTTGGGGAA 
ATGGCAGATG CGTTCAAATC TGATTACTTC AACATGCCAG TCCATATGGT CCCCACAGAG 
CTTGTTGAGA AAGAATTTTG GAGACTAGTA AGCACTATTG AGGAGGATGT CACAGTGGAA 
TATGGAGCTG ACATTGCCTC AAAGGAATTT GGCAGTGGCT TTCCTGTCCG AGATGGGAAA 
ATCAAACTCT CACCTGAGGA AGAGGAGTAT CTTGATAGTG GCTGGAATTT GAACAACATG 
CCAGTGATGG AGCAGTCTGT CCTTGCACAT ATTACTGCTG ATATATGTGG CATGAAACTT 
CCTTGGTTGT ATGTGGGAAT GTGCTTTTCT TCATTCTGTT GGCACATTGA AGACCACTGG 
AGCTATTCAA TTAACTACTT GCACTGGGGT GAGCCAAAAA CCTGGTATGG AGTCCCAGGG 
TATGCTGCTG AGCAGCTAGA AAATGTAATG AAGAAACTAG CTCCAGAACT CTTTGTGTCC 
CAGCCGGATC TCCTCCATCA GCTTGTGACC ATCATGAACC CCAATACCCT GATGACTCAT 
GAAGTGCCTG TTTACCGAAC TAATCAGTGT GCTGGGGAGT TTGTGATTAC ATTTCCAAGA 
GCCTACCACA GTGGTTTTAA CCAGGGTTTT AATTTTGCTG AGGCTGTTAA CTTCTGCACT 
GTTGATTGGC TGCCATTAGG CCGACAGTGT GTGGAGCATT ATCGCTTGCT TCATCGATAT 
TGTGTGTTTT CCCACGATGA GATGATCTGC AAGATGGCTT CCAAGGCTGA TGTATTAGAT 
GTTGTAGTGG CTTCAACTGT TCAGAAAGAC ATGGCCATTA TGATTGAGGA TGAGAAAGCT 
TTAAGAGAAA CTGTCCGTAA ATTGGGAGTG ATTGATTCGG AAAGAATGGA TTTTGAGCTG 
TTGCCAGATG ATGAACGTCA GTGTGTAAAA TGCAAAACTA CATGCTTCAT GTCTGCCATC 
TCCTGTTCTT GTAAACCTGG CCTTCTTGTT TGCCTGCATC ATGTAAAAGA ATTGTGTTCC 
TGTCCTCCTT ACAAATATAA ATTGCGGTAT AGGTACACGC TGGATGATCT CTACCCTATG 
ATGAATGCAT TGAAGCTTCG AGCAGAATCT TACAACGAAT GGGCCTTGAA TGTGAATGAA 
GCTTTGGAGG CAAAGATCAA CAAGAAGAAA AGCCTTGTCA GCTTCAAGGC TTTAATTGAA 
GAATCTGAAA TGAAGAAATT CCCAGACAAT GATCTTTTGC GACACCTTCG CCTAGTCACA 
CAGGATGCAG AGAAGTGTGC CTCTGTTGCG CAGCAGTTGC TTAATGGCAA AAGGCAAACT 
AGATATCGAT CTGGTGGAGG GAAATCCCAA AATCAGTTGA CAGTGAATGA GCTCCGGCAG 
TTTGTAACAC AGCTGTATGC TCTTCCATGT GTCCTCAGTC AGACACCATT ACTAAAGGAT 
CTCTTGAATC GTGTAGAAGA TTTTCAACAG CATAGTCAGA AACTACTCTC TGAGGAAACG 
CCTAGTGCTG CGGAGCTGCA GGACTTGCTA GATGTCAGCT TTGAATTTGA TGTTGAACTT 
CCACAGCTTG CTGAGATGCG TATCCGTTTG GAACAAGCCC GTTGGCTAGA AGAGGTGCAG 
CAAGCTTGCC TAGACCCCAG CTCCCTTACT TTAGATGATA TGAGACGTCT CATAGACCTA 
GGGGTAGGGC TGGCCCCGTA TTCAGCAGTG GAGAAAGCTA TGGCCCGGCT GCAGGAACTG 
CTCACAGTGT CAGAGCACTG GGACGACAAA GCCAAGAGTC TCCTCAAGGC CAGGCCACGA 
CATTCATTGA ATAGCCTTGC TACGGCAGTA AAGGAAATCG AAGAGATCCC TGCATATCTG 
CCCAATGGTG CGGCTCTGAA AGACTCAGTG CAGAGAGCCA GAGACTGGCT TCAGGATGTA 
GAGGGCCTGC AGGCTGGAGG ACGTGTGCCA GTGTTAGACA CACTCATAGA ACTTGTTACA 
CGAGGCCGAT CTATCCCCGT ACATCTGAAT TCTTTGCCAA GACTGGAAAC CCTAGTAGCT 
GAGGTTCAGG CTTGGAAAGA ATGTGCTGTT AATACATTCT TGACTGAGAA TTCTCCATAT 
TCTCTCTTAG AGGTGCTGTG TCCTCGATGT GATATTGGCC TTTTGGGATT GAAAAGGAAG 
CAGAGAAAGT TAAAGGAGCC CTTGCCAAAT GGAAAGAAAA AAAGCACCAA ATTAGAGAGT 
CTGAGTGACC TGGAGAGAGC TTTAACTGAA AGCAAGGAGA CTGCTTCAGC TATGGCAACT 
CTTGGGGAAG CTCGCCTAAG GGAAATGGAA GCCTTGCAGT CTCTCAGACT CGCCAATGAA 
GGGAAATTGC TGTCGCCTCT CCAAGATGTG GATATAAAAA TCTGCCTATG TCAGAAGGCC 
CCAGCTGCCC CTATGATTCA ATGTGAACTC TGCAGGGATG CTTTCCACAC CAGTTGTGTG 
GCGGTACCCA GTATTTCACA GGGCCTGCGA ATCTGGCTTT GTCCCCATTG TCGGAGGTCA 
GAGAAACCTC CATTAGAGAA AATTCTGCCC CTGCTCGCCT CCCTTCAGCG TATCCGAGTT 
CGCCTTCCTG AGGGAGATGC ACTTCGATAT ATGATTGAAA GAACCGTGAA CTGGCAGCAC 
AGAGCCCAGC AACTGCTTTC GTCAGGGAAT CTTAAATTTG TGCAAGATCG AGTGGGCTCA 
GGACTGTTAT ATAGCAGATG GCAAGCCTCA GCAGGACAGG TGTCAGACAC AAACAAGGTA 
TCTCAACCTC CTGGCACAAC ATCATTTTCT TTGCCTGATG ACTGGGACAA CAGAACCTCA 
TATTTGCACT CCCCCTTCTC AACTGGACGA AGTTGTATCC CCCTCCATGG TGTTAGTCCA 
GAAGTGAATG AACTATTGAT GGAAGCCCAG CTGCTCCAGG TATCCCTTCC TGAAATTCAG 
GAACTTTACC AGACTTTACT TGCAAAGCCA AGCCCTGCTC AGCAGACTGA CCGAAGCTCA 
CCAGTGAGAC CCAGCAGTGA GAAGAATGAC TGTTGCCGAG GGAAGCGAGA TGGAATTAAC 
AGTCTTGAGA GAAAACTGAA GAGACGCCTG GAAAGAGAGG GCCTCTCCAG TGAGCGGTGG 
GAACGAGTTA AGAAAATGCG GACCCCCAAA AAGAAGAAAA TCAAACTGAG CCACCCCAAG 
GACATGAACA ATTTCAAGTT AGAGAGAGAG CGTAGCTATG AATTAGTTCG TTCTGCTGAA 
ACTCATTCCC TGCCCTCAGA CACATCCTAT TCCGAACAGG AAGACTCTGA GGATGAAGAT 
GCCATCTGCC CAGCTGTGAG CTGCCTGCAG CCAGAAGGAG ATGAGGTGGA CTGGGTCCAG 
TGTGATGGCA GCTGCAATCA GTGGTTTCAT CAGGTCTGTG TTGGTGTCTC CCCAGAGATG 
GCAGAGAAAG AAGACTACAT CTGTGTGCGC TGTACTGTGA AGGACGCACC AAGCCGAAAG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM5B ( XM_011509090.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011507392.1 Homo sapiens lysine demethylase 5B (KDM5B), transcript variant X2, mRNA.

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CloneID	OHu64359
Clone ID Related Accession (Same CDS sequence)	XM_011509090.2 , XM_011509091.2 , XM_011509090.3
Accession Version	XM_011509090.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4269bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	lysine-specific demethylase 5B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011509090.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGTTTG CTCCTGGCAA AGCAGTGGGC TCACATATCA GAGGGCATTA TGAACGAATT 
CTCAACCCCT ACAACTTATT CCTGTCCGGA GACAGCCTAA GGTGTTTGCA GAAGCCAAAC 
CTGACCACAG ACACTAAGGA CAAGGAGTAC AAACCCCATG ATATTCCCCA GAGGCAGTCT 
GTGCAGCCTT CGGAAACGTG CCCCCCAGCC CGACGAGCAA AACGCATGAG AGCAGAGAGA 
CAGAGTCTCG CTGTGTTGCC CAGGCTGGAG TGCAGTGGCG CGATCTTGGC TCACTGCAAC 
CTCCGCCTCC TAGATTCCAG CAATTCTTCT GCCTCAGCCT CCCAAGCCAT GAATATTAAA 
ATAGAACCCG AGGAGACAAC GGAAGCCAGA ACTCATAATC TGAGACGTCG AATGGGTTGT 
CCAACTCCAA AATGTGAAAA TGAGAAAGAA ATGAAGAGTA GCATCAAGCA AGAACCTATT 
GAGAGGAAAG ATTATATTGT AGAAAATGAG AAGGAAAAGC CCAAGAGTCG ATCTAAAAAA 
GCCACCAATG CTGTGGACCT GTATGTCTGT CTTTTATGTG GCAGTGGCAA TGATGAAGAC 
CGGCTACTGT TGTGTGATGG CTGTGATGAC AGTTACCATA CCTTTTGCTT GATCCCACCT 
CTCCATGATG TTCCCAAGGG AGACTGGAGG TGTCCTAAGT GTTTGGCTCA GGAATGTAGT 
AAGCCACAAG AAGCATTTGG CTTTGAACAA GCAGCCAGGG ACTATACCCT CCGTACTTTT 
GGGGAAATGG CAGATGCGTT CAAATCTGAT TACTTCAACA TGCCAGTCCA TATGGTCCCC 
ACAGAGCTTG TTGAGAAAGA ATTTTGGAGA CTAGTAAGCA CTATTGAGGA GGATGTCACA 
GTGGAATATG GAGCTGACAT TGCCTCAAAG GAATTTGGCA GTGGCTTTCC TGTCCGAGAT 
GGGAAAATCA AACTCTCACC TGAGGAAGAG GAGTATCTTG ATAGTGGCTG GAATTTGAAC 
AACATGCCAG TGATGGAGCA GTCTGTCCTT GCACATATTA CTGCTGATAT ATGTGGCATG 
AAACTTCCTT GGTTGTATGT GGGAATGTGC TTTTCTTCAT TCTGTTGGCA CATTGAAGAC 
CACTGGAGCT ATTCAATTAA CTACTTGCAC TGGGGTGAGC CAAAAACCTG GTATGGAGTC 
CCAGGGTATG CTGCTGAGCA GCTAGAAAAT GTAATGAAGA AACTAGCTCC AGAACTCTTT 
GTGTCCCAGC CGGATCTCCT CCATCAGCTT GTGACCATCA TGAACCCCAA TACCCTGATG 
ACTCATGAAG TGCCTGTTTA CCGAACTAAT CAGTGTGCTG GGGAGTTTGT GATTACATTT 
CCAAGAGCCT ACCACAGTGG TTTTAACCAG GGTTTTAATT TTGCTGAGGC TGTTAACTTC 
TGCACTGTTG ATTGGCTGCC ATTAGGCCGA CAGTGTGTGG AGCATTATCG CTTGCTTCAT 
CGATATTGTG TGTTTTCCCA CGATGAGATG ATCTGCAAGA TGGCTTCCAA GGCTGATGTA 
TTAGATGTTG TAGTGGCTTC AACTGTTCAG AAAGACATGG CCATTATGAT TGAGGATGAG 
AAAGCTTTAA GAGAAACTGT CCGTAAATTG GGAGTGATTG ATTCGGAAAG AATGGATTTT 
GAGCTGTTGC CAGATGATGA ACGTCAGTGT GTAAAATGCA AAACTACATG CTTCATGTCT 
GCCATCTCCT GTTCTTGTAA ACCTGGCCTT CTTGTTTGCC TGCATCATGT AAAAGAATTG 
TGTTCCTGTC CTCCTTACAA ATATAAATTG CGGTATAGGT ACACGCTGGA TGATCTCTAC 
CCTATGATGA ATGCATTGAA GCTTCGAGCA GAATCTTACA ACGAATGGGC CTTGAATGTG 
AATGAAGCTT TGGAGGCAAA GATCAACAAG AAGAAAAGCC TTGTCAGCTT CAAGGCTTTA 
ATTGAAGAAT CTGAAATGAA GAAATTCCCA GACAATGATC TTTTGCGACA CCTTCGCCTA 
GTCACACAGG ATGCAGAGAA GTGTGCCTCT GTTGCGCAGC AGTTGCTTAA TGGCAAAAGG 
CAAACTAGAT ATCGATCTGG TGGAGGGAAA TCCCAAAATC AGTTGACAGT GAATGAGCTC 
CGGCAGTTTG TAACACAGCT GTATGCTCTT CCATGTGTCC TCAGTCAGAC ACCATTACTA 
AAGGATCTCT TGAATCGTGT AGAAGATTTT CAACAGCATA GTCAGAAACT ACTCTCTGAG 
GAAACGCCTA GTGCTGCGGA GCTGCAGGAC TTGCTAGATG TCAGCTTTGA ATTTGATGTT 
GAACTTCCAC AGCTTGCTGA GATGCGTATC CGTTTGGAAC AAGCCCGTTG GCTAGAAGAG 
GTGCAGCAAG CTTGCCTAGA CCCCAGCTCC CTTACTTTAG ATGATATGAG ACGTCTCATA 
GACCTAGGGG TAGGGCTGGC CCCGTATTCA GCAGTGGAGA AAGCTATGGC CCGGCTGCAG 
GAACTGCTCA CAGTGTCAGA GCACTGGGAC GACAAAGCCA AGAGTCTCCT CAAGGCCAGG 
CCACGACATT CATTGAATAG CCTTGCTACG GCAGTAAAGG AAATCGAAGA GATCCCTGCA 
TATCTGCCCA ATGGTGCGGC TCTGAAAGAC TCAGTGCAGA GAGCCAGAGA CTGGCTTCAG 
GATGTAGAGG GCCTGCAGGC TGGAGGACGT GTGCCAGTGT TAGACACACT CATAGAACTT 
GTTACACGAG GCCGATCTAT CCCCGTACAT CTGAATTCTT TGCCAAGACT GGAAACCCTA 
GTAGCTGAGG TTCAGGCTTG GAAAGAATGT GCTGTTAATA CATTCTTGAC TGAGAATTCT 
CCATATTCTC TCTTAGAGGT GCTGTGTCCT CGATGTGATA TTGGCCTTTT GGGATTGAAA 
AGGAAGCAGA GAAAGTTAAA GGAGCCCTTG CCAAATGGAA AGAAAAAAAG CACCAAATTA 
GAGAGTCTGA GTGACCTGGA GAGAGCTTTA ACTGAAAGCA AGGAGACTGC TTCAGCTATG 
GCAACTCTTG GGGAAGCTCG CCTAAGGGAA ATGGAAGCCT TGCAGTCTCT CAGACTCGCC 
AATGAAGGGA AATTGCTGTC GCCTCTCCAA GATGTGGATA TAAAAATCTG CCTATGTCAG 
AAGGCCCCAG CTGCCCCTAT GATTCAATGT GAACTCTGCA GGGATGCTTT CCACACCAGT 
TGTGTGGCGG TACCCAGTAT TTCACAGGGC CTGCGAATCT GGCTTTGTCC CCATTGTCGG 
AGGTCAGAGA AACCTCCATT AGAGAAAATT CTGCCCCTGC TCGCCTCCCT TCAGCGTATC 
CGAGTTCGCC TTCCTGAGGG AGATGCACTT CGATATATGA TTGAAAGAAC CGTGAACTGG 
CAGCACAGAG CCCAGCAACT GCTTTCGTCA GGGAATCTTA AATTTGTGCA AGATCGAGTG 
GGCTCAGGAC TGTTATATAG CAGATGGCAA GCCTCAGCAG GACAGGTGTC AGACACAAAC 
AAGGTATCTC AACCTCCTGG CACAACATCA TTTTCTTTGC CTGATGACTG GGACAACAGA 
ACCTCATATT TGCACTCCCC CTTCTCAACT GGACGAAGTT GTATCCCCCT CCATGGTGTT 
AGTCCAGAAG TGAATGAACT ATTGATGGAA GCCCAGCTGC TCCAGGTATC CCTTCCTGAA 
ATTCAGGAAC TTTACCAGAC TTTACTTGCA AAGCCAAGCC CTGCTCAGCA GACTGACCGA 
AGCTCACCAG TGAGACCCAG CAGTGAGAAG AATGACTGTT GCCGAGGGAA GCGAGATGGA 
ATTAACAGTC TTGAGAGAAA ACTGAAGAGA CGCCTGGAAA GAGAGGGCCT CTCCAGTGAG 
CGGTGGGAAC GAGTTAAGAA AATGCGGACC CCCAAAAAGA AGAAAATCAA ACTGAGCCAC 
CCCAAGGACA TGAACAATTT CAAGTTAGAG AGAGAGCGTA GCTATGAATT AGTTCGTTCT 
GCTGAAACTC ATTCCCTGCC CTCAGACACA TCCTATTCCG AACAGGAAGA CTCTGAGGAT 
GAAGATGCCA TCTGCCCAGC TGTGAGCTGC CTGCAGCCAG AAGGAGATGA GGTGGACTGG 
GTCCAGTGTG ATGGCAGCTG CAATCAGTGG TTTCATCAGG TCTGTGTTGG TGTCTCCCCA 
GAGATGGCAG AGAAAGAAGA CTACATCTGT GTGCGCTGTA CTGTGAAGGA CGCACCAAGC 
CGAAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011507392.1
CDS	621..4889
Translation	MGFAPGKAVGSHIRGHYERILNPYNLFLSGDSLRCLQKPNLTTDTKDKEYKPHDIPQRQSVQPSETCPPARRAKRMRAERQSLAVLPRLECSGAILAHCNLRLLDSSNSSASASQAMNIKIEPEETTEARTHNLRRRMGCPTPKCENEKEMKSSIKQEPIERKDYIVENEKEKPKSRSKKATNAVDLYVCLLCGSGNDEDRLLLCDGCDDSYHTFCLIPPLHDVPKGDWRCPKCLAQECSKPQEAFGFEQAARDYTLRTFGEMADAFKSDYFNMPVHMVPTELVEKEFWRLVSTIEEDVTVEYGADIASKEFGSGFPVRDGKIKLSPEEEEYLDSGWNLNNMPVMEQSVLAHITADICGMKLPWLYVGMCFSSFCWHIEDHWSYSINYLHWGEPKTWYGVPGYAAEQLENVMKKLAPELFVSQPDLLHQLVTIMNPNTLMTHEVPVYRTNQCAGEFVITFPRAYHSGFNQGFNFAEAVNFCTVDWLPLGRQCVEHYRLLHRYCVFSHDEMICKMASKADVLDVVVASTVQKDMAIMIEDEKALRETVRKLGVIDSERMDFELLPDDERQCVKCKTTCFMSAISCSCKPGLLVCLHHVKELCSCPPYKYKLRYRYTLDDLYPMMNALKLRAESYNEWALNVNEALEAKINKKKSLVSFKALIEESEMKKFPDNDLLRHLRLVTQDAEKCASVAQQLLNGKRQTRYRSGGGKSQNQLTVNELRQFVTQLYALPCVLSQTPLLKDLLNRVEDFQQHSQKLLSEETPSAAELQDLLDVSFEFDVELPQLAEMRIRLEQARWLEEVQQACLDPSSLTLDDMRRLIDLGVGLAPYSAVEKAMARLQELLTVSEHWDDKAKSLLKARPRHSLNSLATAVKEIEEIPAYLPNGAALKDSVQRARDWLQDVEGLQAGGRVPVLDTLIELVTRGRSIPVHLNSLPRLETLVAEVQAWKECAVNTFLTENSPYSLLEVLCPRCDIGLLGLKRKQRKLKEPLPNGKKKSTKLESLSDLERALTESKETASAMATLGEARLREMEALQSLRLANEGKLLSPLQDVDIKICLCQKAPAAPMIQCELCRDAFHTSCVAVPSISQGLRIWLCPHCRRSEKPPLEKILPLLASLQRIRVRLPEGDALRYMIERTVNWQHRAQQLLSSGNLKFVQDRVGSGLLYSRWQASAGQVSDTNKVSQPPGTTSFSLPDDWDNRTSYLHSPFSTGRSCIPLHGVSPEVNELLMEAQLLQVSLPEIQELYQTLLAKPSPAQQTDRSSPVRPSSEKNDCCRGKRDGINSLERKLKRRLEREGLSSERWERVKKMRTPKKKKIKLSHPKDMNNFKLERERSYELVRSAETHSLPSDTSYSEQEDSEDEDAICPAVSCLQPEGDEVDWVQCDGSCNQWFHQVCVGVSPEMAEKEDYICVRCTVKDAPSRK

Target ORF information:

RefSeq Version	XM_011509090.2
Organism	Homo sapiens(human)
Definition	Homo sapiens lysine demethylase 5B (KDM5B), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011509090.2

ORF Insert Sequence:

ATGGGGTTTG CTCCTGGCAA AGCAGTGGGC TCACATATCA GAGGGCATTA TGAACGAATT 
CTCAACCCCT ACAACTTATT CCTGTCCGGA GACAGCCTAA GGTGTTTGCA GAAGCCAAAC 
CTGACCACAG ACACTAAGGA CAAGGAGTAC AAACCCCATG ATATTCCCCA GAGGCAGTCT 
GTGCAGCCTT CGGAAACGTG CCCCCCAGCC CGACGAGCAA AACGCATGAG AGCAGAGAGA 
CAGAGTCTCG CTGTGTTGCC CAGGCTGGAG TGCAGTGGCG CGATCTTGGC TCACTGCAAC 
CTCCGCCTCC TAGATTCCAG CAATTCTTCT GCCTCAGCCT CCCAAGCCAT GAATATTAAA 
ATAGAACCCG AGGAGACAAC GGAAGCCAGA ACTCATAATC TGAGACGTCG AATGGGTTGT 
CCAACTCCAA AATGTGAAAA TGAGAAAGAA ATGAAGAGTA GCATCAAGCA AGAACCTATT 
GAGAGGAAAG ATTATATTGT AGAAAATGAG AAGGAAAAGC CCAAGAGTCG ATCTAAAAAA 
GCCACCAATG CTGTGGACCT GTATGTCTGT CTTTTATGTG GCAGTGGCAA TGATGAAGAC 
CGGCTACTGT TGTGTGATGG CTGTGATGAC AGTTACCATA CCTTTTGCTT GATCCCACCT 
CTCCATGATG TTCCCAAGGG AGACTGGAGG TGTCCTAAGT GTTTGGCTCA GGAATGTAGT 
AAGCCACAAG AAGCATTTGG CTTTGAACAA GCAGCCAGGG ACTATACCCT CCGTACTTTT 
GGGGAAATGG CAGATGCGTT CAAATCTGAT TACTTCAACA TGCCAGTCCA TATGGTCCCC 
ACAGAGCTTG TTGAGAAAGA ATTTTGGAGA CTAGTAAGCA CTATTGAGGA GGATGTCACA 
GTGGAATATG GAGCTGACAT TGCCTCAAAG GAATTTGGCA GTGGCTTTCC TGTCCGAGAT 
GGGAAAATCA AACTCTCACC TGAGGAAGAG GAGTATCTTG ATAGTGGCTG GAATTTGAAC 
AACATGCCAG TGATGGAGCA GTCTGTCCTT GCACATATTA CTGCTGATAT ATGTGGCATG 
AAACTTCCTT GGTTGTATGT GGGAATGTGC TTTTCTTCAT TCTGTTGGCA CATTGAAGAC 
CACTGGAGCT ATTCAATTAA CTACTTGCAC TGGGGTGAGC CAAAAACCTG GTATGGAGTC 
CCAGGGTATG CTGCTGAGCA GCTAGAAAAT GTAATGAAGA AACTAGCTCC AGAACTCTTT 
GTGTCCCAGC CGGATCTCCT CCATCAGCTT GTGACCATCA TGAACCCCAA TACCCTGATG 
ACTCATGAAG TGCCTGTTTA CCGAACTAAT CAGTGTGCTG GGGAGTTTGT GATTACATTT 
CCAAGAGCCT ACCACAGTGG TTTTAACCAG GGTTTTAATT TTGCTGAGGC TGTTAACTTC 
TGCACTGTTG ATTGGCTGCC ATTAGGCCGA CAGTGTGTGG AGCATTATCG CTTGCTTCAT 
CGATATTGTG TGTTTTCCCA CGATGAGATG ATCTGCAAGA TGGCTTCCAA GGCTGATGTA 
TTAGATGTTG TAGTGGCTTC AACTGTTCAG AAAGACATGG CCATTATGAT TGAGGATGAG 
AAAGCTTTAA GAGAAACTGT CCGTAAATTG GGAGTGATTG ATTCGGAAAG AATGGATTTT 
GAGCTGTTGC CAGATGATGA ACGTCAGTGT GTAAAATGCA AAACTACATG CTTCATGTCT 
GCCATCTCCT GTTCTTGTAA ACCTGGCCTT CTTGTTTGCC TGCATCATGT AAAAGAATTG 
TGTTCCTGTC CTCCTTACAA ATATAAATTG CGGTATAGGT ACACGCTGGA TGATCTCTAC 
CCTATGATGA ATGCATTGAA GCTTCGAGCA GAATCTTACA ACGAATGGGC CTTGAATGTG 
AATGAAGCTT TGGAGGCAAA GATCAACAAG AAGAAAAGCC TTGTCAGCTT CAAGGCTTTA 
ATTGAAGAAT CTGAAATGAA GAAATTCCCA GACAATGATC TTTTGCGACA CCTTCGCCTA 
GTCACACAGG ATGCAGAGAA GTGTGCCTCT GTTGCGCAGC AGTTGCTTAA TGGCAAAAGG 
CAAACTAGAT ATCGATCTGG TGGAGGGAAA TCCCAAAATC AGTTGACAGT GAATGAGCTC 
CGGCAGTTTG TAACACAGCT GTATGCTCTT CCATGTGTCC TCAGTCAGAC ACCATTACTA 
AAGGATCTCT TGAATCGTGT AGAAGATTTT CAACAGCATA GTCAGAAACT ACTCTCTGAG 
GAAACGCCTA GTGCTGCGGA GCTGCAGGAC TTGCTAGATG TCAGCTTTGA ATTTGATGTT 
GAACTTCCAC AGCTTGCTGA GATGCGTATC CGTTTGGAAC AAGCCCGTTG GCTAGAAGAG 
GTGCAGCAAG CTTGCCTAGA CCCCAGCTCC CTTACTTTAG ATGATATGAG ACGTCTCATA 
GACCTAGGGG TAGGGCTGGC CCCGTATTCA GCAGTGGAGA AAGCTATGGC CCGGCTGCAG 
GAACTGCTCA CAGTGTCAGA GCACTGGGAC GACAAAGCCA AGAGTCTCCT CAAGGCCAGG 
CCACGACATT CATTGAATAG CCTTGCTACG GCAGTAAAGG AAATCGAAGA GATCCCTGCA 
TATCTGCCCA ATGGTGCGGC TCTGAAAGAC TCAGTGCAGA GAGCCAGAGA CTGGCTTCAG 
GATGTAGAGG GCCTGCAGGC TGGAGGACGT GTGCCAGTGT TAGACACACT CATAGAACTT 
GTTACACGAG GCCGATCTAT CCCCGTACAT CTGAATTCTT TGCCAAGACT GGAAACCCTA 
GTAGCTGAGG TTCAGGCTTG GAAAGAATGT GCTGTTAATA CATTCTTGAC TGAGAATTCT 
CCATATTCTC TCTTAGAGGT GCTGTGTCCT CGATGTGATA TTGGCCTTTT GGGATTGAAA 
AGGAAGCAGA GAAAGTTAAA GGAGCCCTTG CCAAATGGAA AGAAAAAAAG CACCAAATTA 
GAGAGTCTGA GTGACCTGGA GAGAGCTTTA ACTGAAAGCA AGGAGACTGC TTCAGCTATG 
GCAACTCTTG GGGAAGCTCG CCTAAGGGAA ATGGAAGCCT TGCAGTCTCT CAGACTCGCC 
AATGAAGGGA AATTGCTGTC GCCTCTCCAA GATGTGGATA TAAAAATCTG CCTATGTCAG 
AAGGCCCCAG CTGCCCCTAT GATTCAATGT GAACTCTGCA GGGATGCTTT CCACACCAGT 
TGTGTGGCGG TACCCAGTAT TTCACAGGGC CTGCGAATCT GGCTTTGTCC CCATTGTCGG 
AGGTCAGAGA AACCTCCATT AGAGAAAATT CTGCCCCTGC TCGCCTCCCT TCAGCGTATC 
CGAGTTCGCC TTCCTGAGGG AGATGCACTT CGATATATGA TTGAAAGAAC CGTGAACTGG 
CAGCACAGAG CCCAGCAACT GCTTTCGTCA GGGAATCTTA AATTTGTGCA AGATCGAGTG 
GGCTCAGGAC TGTTATATAG CAGATGGCAA GCCTCAGCAG GACAGGTGTC AGACACAAAC 
AAGGTATCTC AACCTCCTGG CACAACATCA TTTTCTTTGC CTGATGACTG GGACAACAGA 
ACCTCATATT TGCACTCCCC CTTCTCAACT GGACGAAGTT GTATCCCCCT CCATGGTGTT 
AGTCCAGAAG TGAATGAACT ATTGATGGAA GCCCAGCTGC TCCAGGTATC CCTTCCTGAA 
ATTCAGGAAC TTTACCAGAC TTTACTTGCA AAGCCAAGCC CTGCTCAGCA GACTGACCGA 
AGCTCACCAG TGAGACCCAG CAGTGAGAAG AATGACTGTT GCCGAGGGAA GCGAGATGGA 
ATTAACAGTC TTGAGAGAAA ACTGAAGAGA CGCCTGGAAA GAGAGGGCCT CTCCAGTGAG 
CGGTGGGAAC GAGTTAAGAA AATGCGGACC CCCAAAAAGA AGAAAATCAA ACTGAGCCAC 
CCCAAGGACA TGAACAATTT CAAGTTAGAG AGAGAGCGTA GCTATGAATT AGTTCGTTCT 
GCTGAAACTC ATTCCCTGCC CTCAGACACA TCCTATTCCG AACAGGAAGA CTCTGAGGAT 
GAAGATGCCA TCTGCCCAGC TGTGAGCTGC CTGCAGCCAG AAGGAGATGA GGTGGACTGG 
GTCCAGTGTG ATGGCAGCTG CAATCAGTGG TTTCATCAGG TCTGTGTTGG TGTCTCCCCA 
GAGATGGCAG AGAAAGAAGA CTACATCTGT GTGCGCTGTA CTGTGAAGGA CGCACCAAGC 
CGAAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM5B ( XM_011509091.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011507393.1 Homo sapiens lysine demethylase 5B (KDM5B), transcript variant X2, mRNA.

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CloneID	OHu64359
Clone ID Related Accession (Same CDS sequence)	XM_011509090.2 , XM_011509091.2 , XM_011509090.3
Accession Version	XM_011509091.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4269bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	lysine-specific demethylase 5B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011509091.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGTTTG CTCCTGGCAA AGCAGTGGGC TCACATATCA GAGGGCATTA TGAACGAATT 
CTCAACCCCT ACAACTTATT CCTGTCCGGA GACAGCCTAA GGTGTTTGCA GAAGCCAAAC 
CTGACCACAG ACACTAAGGA CAAGGAGTAC AAACCCCATG ATATTCCCCA GAGGCAGTCT 
GTGCAGCCTT CGGAAACGTG CCCCCCAGCC CGACGAGCAA AACGCATGAG AGCAGAGAGA 
CAGAGTCTCG CTGTGTTGCC CAGGCTGGAG TGCAGTGGCG CGATCTTGGC TCACTGCAAC 
CTCCGCCTCC TAGATTCCAG CAATTCTTCT GCCTCAGCCT CCCAAGCCAT GAATATTAAA 
ATAGAACCCG AGGAGACAAC GGAAGCCAGA ACTCATAATC TGAGACGTCG AATGGGTTGT 
CCAACTCCAA AATGTGAAAA TGAGAAAGAA ATGAAGAGTA GCATCAAGCA AGAACCTATT 
GAGAGGAAAG ATTATATTGT AGAAAATGAG AAGGAAAAGC CCAAGAGTCG ATCTAAAAAA 
GCCACCAATG CTGTGGACCT GTATGTCTGT CTTTTATGTG GCAGTGGCAA TGATGAAGAC 
CGGCTACTGT TGTGTGATGG CTGTGATGAC AGTTACCATA CCTTTTGCTT GATCCCACCT 
CTCCATGATG TTCCCAAGGG AGACTGGAGG TGTCCTAAGT GTTTGGCTCA GGAATGTAGT 
AAGCCACAAG AAGCATTTGG CTTTGAACAA GCAGCCAGGG ACTATACCCT CCGTACTTTT 
GGGGAAATGG CAGATGCGTT CAAATCTGAT TACTTCAACA TGCCAGTCCA TATGGTCCCC 
ACAGAGCTTG TTGAGAAAGA ATTTTGGAGA CTAGTAAGCA CTATTGAGGA GGATGTCACA 
GTGGAATATG GAGCTGACAT TGCCTCAAAG GAATTTGGCA GTGGCTTTCC TGTCCGAGAT 
GGGAAAATCA AACTCTCACC TGAGGAAGAG GAGTATCTTG ATAGTGGCTG GAATTTGAAC 
AACATGCCAG TGATGGAGCA GTCTGTCCTT GCACATATTA CTGCTGATAT ATGTGGCATG 
AAACTTCCTT GGTTGTATGT GGGAATGTGC TTTTCTTCAT TCTGTTGGCA CATTGAAGAC 
CACTGGAGCT ATTCAATTAA CTACTTGCAC TGGGGTGAGC CAAAAACCTG GTATGGAGTC 
CCAGGGTATG CTGCTGAGCA GCTAGAAAAT GTAATGAAGA AACTAGCTCC AGAACTCTTT 
GTGTCCCAGC CGGATCTCCT CCATCAGCTT GTGACCATCA TGAACCCCAA TACCCTGATG 
ACTCATGAAG TGCCTGTTTA CCGAACTAAT CAGTGTGCTG GGGAGTTTGT GATTACATTT 
CCAAGAGCCT ACCACAGTGG TTTTAACCAG GGTTTTAATT TTGCTGAGGC TGTTAACTTC 
TGCACTGTTG ATTGGCTGCC ATTAGGCCGA CAGTGTGTGG AGCATTATCG CTTGCTTCAT 
CGATATTGTG TGTTTTCCCA CGATGAGATG ATCTGCAAGA TGGCTTCCAA GGCTGATGTA 
TTAGATGTTG TAGTGGCTTC AACTGTTCAG AAAGACATGG CCATTATGAT TGAGGATGAG 
AAAGCTTTAA GAGAAACTGT CCGTAAATTG GGAGTGATTG ATTCGGAAAG AATGGATTTT 
GAGCTGTTGC CAGATGATGA ACGTCAGTGT GTAAAATGCA AAACTACATG CTTCATGTCT 
GCCATCTCCT GTTCTTGTAA ACCTGGCCTT CTTGTTTGCC TGCATCATGT AAAAGAATTG 
TGTTCCTGTC CTCCTTACAA ATATAAATTG CGGTATAGGT ACACGCTGGA TGATCTCTAC 
CCTATGATGA ATGCATTGAA GCTTCGAGCA GAATCTTACA ACGAATGGGC CTTGAATGTG 
AATGAAGCTT TGGAGGCAAA GATCAACAAG AAGAAAAGCC TTGTCAGCTT CAAGGCTTTA 
ATTGAAGAAT CTGAAATGAA GAAATTCCCA GACAATGATC TTTTGCGACA CCTTCGCCTA 
GTCACACAGG ATGCAGAGAA GTGTGCCTCT GTTGCGCAGC AGTTGCTTAA TGGCAAAAGG 
CAAACTAGAT ATCGATCTGG TGGAGGGAAA TCCCAAAATC AGTTGACAGT GAATGAGCTC 
CGGCAGTTTG TAACACAGCT GTATGCTCTT CCATGTGTCC TCAGTCAGAC ACCATTACTA 
AAGGATCTCT TGAATCGTGT AGAAGATTTT CAACAGCATA GTCAGAAACT ACTCTCTGAG 
GAAACGCCTA GTGCTGCGGA GCTGCAGGAC TTGCTAGATG TCAGCTTTGA ATTTGATGTT 
GAACTTCCAC AGCTTGCTGA GATGCGTATC CGTTTGGAAC AAGCCCGTTG GCTAGAAGAG 
GTGCAGCAAG CTTGCCTAGA CCCCAGCTCC CTTACTTTAG ATGATATGAG ACGTCTCATA 
GACCTAGGGG TAGGGCTGGC CCCGTATTCA GCAGTGGAGA AAGCTATGGC CCGGCTGCAG 
GAACTGCTCA CAGTGTCAGA GCACTGGGAC GACAAAGCCA AGAGTCTCCT CAAGGCCAGG 
CCACGACATT CATTGAATAG CCTTGCTACG GCAGTAAAGG AAATCGAAGA GATCCCTGCA 
TATCTGCCCA ATGGTGCGGC TCTGAAAGAC TCAGTGCAGA GAGCCAGAGA CTGGCTTCAG 
GATGTAGAGG GCCTGCAGGC TGGAGGACGT GTGCCAGTGT TAGACACACT CATAGAACTT 
GTTACACGAG GCCGATCTAT CCCCGTACAT CTGAATTCTT TGCCAAGACT GGAAACCCTA 
GTAGCTGAGG TTCAGGCTTG GAAAGAATGT GCTGTTAATA CATTCTTGAC TGAGAATTCT 
CCATATTCTC TCTTAGAGGT GCTGTGTCCT CGATGTGATA TTGGCCTTTT GGGATTGAAA 
AGGAAGCAGA GAAAGTTAAA GGAGCCCTTG CCAAATGGAA AGAAAAAAAG CACCAAATTA 
GAGAGTCTGA GTGACCTGGA GAGAGCTTTA ACTGAAAGCA AGGAGACTGC TTCAGCTATG 
GCAACTCTTG GGGAAGCTCG CCTAAGGGAA ATGGAAGCCT TGCAGTCTCT CAGACTCGCC 
AATGAAGGGA AATTGCTGTC GCCTCTCCAA GATGTGGATA TAAAAATCTG CCTATGTCAG 
AAGGCCCCAG CTGCCCCTAT GATTCAATGT GAACTCTGCA GGGATGCTTT CCACACCAGT 
TGTGTGGCGG TACCCAGTAT TTCACAGGGC CTGCGAATCT GGCTTTGTCC CCATTGTCGG 
AGGTCAGAGA AACCTCCATT AGAGAAAATT CTGCCCCTGC TCGCCTCCCT TCAGCGTATC 
CGAGTTCGCC TTCCTGAGGG AGATGCACTT CGATATATGA TTGAAAGAAC CGTGAACTGG 
CAGCACAGAG CCCAGCAACT GCTTTCGTCA GGGAATCTTA AATTTGTGCA AGATCGAGTG 
GGCTCAGGAC TGTTATATAG CAGATGGCAA GCCTCAGCAG GACAGGTGTC AGACACAAAC 
AAGGTATCTC AACCTCCTGG CACAACATCA TTTTCTTTGC CTGATGACTG GGACAACAGA 
ACCTCATATT TGCACTCCCC CTTCTCAACT GGACGAAGTT GTATCCCCCT CCATGGTGTT 
AGTCCAGAAG TGAATGAACT ATTGATGGAA GCCCAGCTGC TCCAGGTATC CCTTCCTGAA 
ATTCAGGAAC TTTACCAGAC TTTACTTGCA AAGCCAAGCC CTGCTCAGCA GACTGACCGA 
AGCTCACCAG TGAGACCCAG CAGTGAGAAG AATGACTGTT GCCGAGGGAA GCGAGATGGA 
ATTAACAGTC TTGAGAGAAA ACTGAAGAGA CGCCTGGAAA GAGAGGGCCT CTCCAGTGAG 
CGGTGGGAAC GAGTTAAGAA AATGCGGACC CCCAAAAAGA AGAAAATCAA ACTGAGCCAC 
CCCAAGGACA TGAACAATTT CAAGTTAGAG AGAGAGCGTA GCTATGAATT AGTTCGTTCT 
GCTGAAACTC ATTCCCTGCC CTCAGACACA TCCTATTCCG AACAGGAAGA CTCTGAGGAT 
GAAGATGCCA TCTGCCCAGC TGTGAGCTGC CTGCAGCCAG AAGGAGATGA GGTGGACTGG 
GTCCAGTGTG ATGGCAGCTG CAATCAGTGG TTTCATCAGG TCTGTGTTGG TGTCTCCCCA 
GAGATGGCAG AGAAAGAAGA CTACATCTGT GTGCGCTGTA CTGTGAAGGA CGCACCAAGC 
CGAAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011507393.1
CDS	358..4626
Translation	MGFAPGKAVGSHIRGHYERILNPYNLFLSGDSLRCLQKPNLTTDTKDKEYKPHDIPQRQSVQPSETCPPARRAKRMRAERQSLAVLPRLECSGAILAHCNLRLLDSSNSSASASQAMNIKIEPEETTEARTHNLRRRMGCPTPKCENEKEMKSSIKQEPIERKDYIVENEKEKPKSRSKKATNAVDLYVCLLCGSGNDEDRLLLCDGCDDSYHTFCLIPPLHDVPKGDWRCPKCLAQECSKPQEAFGFEQAARDYTLRTFGEMADAFKSDYFNMPVHMVPTELVEKEFWRLVSTIEEDVTVEYGADIASKEFGSGFPVRDGKIKLSPEEEEYLDSGWNLNNMPVMEQSVLAHITADICGMKLPWLYVGMCFSSFCWHIEDHWSYSINYLHWGEPKTWYGVPGYAAEQLENVMKKLAPELFVSQPDLLHQLVTIMNPNTLMTHEVPVYRTNQCAGEFVITFPRAYHSGFNQGFNFAEAVNFCTVDWLPLGRQCVEHYRLLHRYCVFSHDEMICKMASKADVLDVVVASTVQKDMAIMIEDEKALRETVRKLGVIDSERMDFELLPDDERQCVKCKTTCFMSAISCSCKPGLLVCLHHVKELCSCPPYKYKLRYRYTLDDLYPMMNALKLRAESYNEWALNVNEALEAKINKKKSLVSFKALIEESEMKKFPDNDLLRHLRLVTQDAEKCASVAQQLLNGKRQTRYRSGGGKSQNQLTVNELRQFVTQLYALPCVLSQTPLLKDLLNRVEDFQQHSQKLLSEETPSAAELQDLLDVSFEFDVELPQLAEMRIRLEQARWLEEVQQACLDPSSLTLDDMRRLIDLGVGLAPYSAVEKAMARLQELLTVSEHWDDKAKSLLKARPRHSLNSLATAVKEIEEIPAYLPNGAALKDSVQRARDWLQDVEGLQAGGRVPVLDTLIELVTRGRSIPVHLNSLPRLETLVAEVQAWKECAVNTFLTENSPYSLLEVLCPRCDIGLLGLKRKQRKLKEPLPNGKKKSTKLESLSDLERALTESKETASAMATLGEARLREMEALQSLRLANEGKLLSPLQDVDIKICLCQKAPAAPMIQCELCRDAFHTSCVAVPSISQGLRIWLCPHCRRSEKPPLEKILPLLASLQRIRVRLPEGDALRYMIERTVNWQHRAQQLLSSGNLKFVQDRVGSGLLYSRWQASAGQVSDTNKVSQPPGTTSFSLPDDWDNRTSYLHSPFSTGRSCIPLHGVSPEVNELLMEAQLLQVSLPEIQELYQTLLAKPSPAQQTDRSSPVRPSSEKNDCCRGKRDGINSLERKLKRRLEREGLSSERWERVKKMRTPKKKKIKLSHPKDMNNFKLERERSYELVRSAETHSLPSDTSYSEQEDSEDEDAICPAVSCLQPEGDEVDWVQCDGSCNQWFHQVCVGVSPEMAEKEDYICVRCTVKDAPSRK

Target ORF information:

RefSeq Version	XM_011509091.2
Organism	Homo sapiens(human)
Definition	Homo sapiens lysine demethylase 5B (KDM5B), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011509091.2

ORF Insert Sequence:

ATGGGGTTTG CTCCTGGCAA AGCAGTGGGC TCACATATCA GAGGGCATTA TGAACGAATT 
CTCAACCCCT ACAACTTATT CCTGTCCGGA GACAGCCTAA GGTGTTTGCA GAAGCCAAAC 
CTGACCACAG ACACTAAGGA CAAGGAGTAC AAACCCCATG ATATTCCCCA GAGGCAGTCT 
GTGCAGCCTT CGGAAACGTG CCCCCCAGCC CGACGAGCAA AACGCATGAG AGCAGAGAGA 
CAGAGTCTCG CTGTGTTGCC CAGGCTGGAG TGCAGTGGCG CGATCTTGGC TCACTGCAAC 
CTCCGCCTCC TAGATTCCAG CAATTCTTCT GCCTCAGCCT CCCAAGCCAT GAATATTAAA 
ATAGAACCCG AGGAGACAAC GGAAGCCAGA ACTCATAATC TGAGACGTCG AATGGGTTGT 
CCAACTCCAA AATGTGAAAA TGAGAAAGAA ATGAAGAGTA GCATCAAGCA AGAACCTATT 
GAGAGGAAAG ATTATATTGT AGAAAATGAG AAGGAAAAGC CCAAGAGTCG ATCTAAAAAA 
GCCACCAATG CTGTGGACCT GTATGTCTGT CTTTTATGTG GCAGTGGCAA TGATGAAGAC 
CGGCTACTGT TGTGTGATGG CTGTGATGAC AGTTACCATA CCTTTTGCTT GATCCCACCT 
CTCCATGATG TTCCCAAGGG AGACTGGAGG TGTCCTAAGT GTTTGGCTCA GGAATGTAGT 
AAGCCACAAG AAGCATTTGG CTTTGAACAA GCAGCCAGGG ACTATACCCT CCGTACTTTT 
GGGGAAATGG CAGATGCGTT CAAATCTGAT TACTTCAACA TGCCAGTCCA TATGGTCCCC 
ACAGAGCTTG TTGAGAAAGA ATTTTGGAGA CTAGTAAGCA CTATTGAGGA GGATGTCACA 
GTGGAATATG GAGCTGACAT TGCCTCAAAG GAATTTGGCA GTGGCTTTCC TGTCCGAGAT 
GGGAAAATCA AACTCTCACC TGAGGAAGAG GAGTATCTTG ATAGTGGCTG GAATTTGAAC 
AACATGCCAG TGATGGAGCA GTCTGTCCTT GCACATATTA CTGCTGATAT ATGTGGCATG 
AAACTTCCTT GGTTGTATGT GGGAATGTGC TTTTCTTCAT TCTGTTGGCA CATTGAAGAC 
CACTGGAGCT ATTCAATTAA CTACTTGCAC TGGGGTGAGC CAAAAACCTG GTATGGAGTC 
CCAGGGTATG CTGCTGAGCA GCTAGAAAAT GTAATGAAGA AACTAGCTCC AGAACTCTTT 
GTGTCCCAGC CGGATCTCCT CCATCAGCTT GTGACCATCA TGAACCCCAA TACCCTGATG 
ACTCATGAAG TGCCTGTTTA CCGAACTAAT CAGTGTGCTG GGGAGTTTGT GATTACATTT 
CCAAGAGCCT ACCACAGTGG TTTTAACCAG GGTTTTAATT TTGCTGAGGC TGTTAACTTC 
TGCACTGTTG ATTGGCTGCC ATTAGGCCGA CAGTGTGTGG AGCATTATCG CTTGCTTCAT 
CGATATTGTG TGTTTTCCCA CGATGAGATG ATCTGCAAGA TGGCTTCCAA GGCTGATGTA 
TTAGATGTTG TAGTGGCTTC AACTGTTCAG AAAGACATGG CCATTATGAT TGAGGATGAG 
AAAGCTTTAA GAGAAACTGT CCGTAAATTG GGAGTGATTG ATTCGGAAAG AATGGATTTT 
GAGCTGTTGC CAGATGATGA ACGTCAGTGT GTAAAATGCA AAACTACATG CTTCATGTCT 
GCCATCTCCT GTTCTTGTAA ACCTGGCCTT CTTGTTTGCC TGCATCATGT AAAAGAATTG 
TGTTCCTGTC CTCCTTACAA ATATAAATTG CGGTATAGGT ACACGCTGGA TGATCTCTAC 
CCTATGATGA ATGCATTGAA GCTTCGAGCA GAATCTTACA ACGAATGGGC CTTGAATGTG 
AATGAAGCTT TGGAGGCAAA GATCAACAAG AAGAAAAGCC TTGTCAGCTT CAAGGCTTTA 
ATTGAAGAAT CTGAAATGAA GAAATTCCCA GACAATGATC TTTTGCGACA CCTTCGCCTA 
GTCACACAGG ATGCAGAGAA GTGTGCCTCT GTTGCGCAGC AGTTGCTTAA TGGCAAAAGG 
CAAACTAGAT ATCGATCTGG TGGAGGGAAA TCCCAAAATC AGTTGACAGT GAATGAGCTC 
CGGCAGTTTG TAACACAGCT GTATGCTCTT CCATGTGTCC TCAGTCAGAC ACCATTACTA 
AAGGATCTCT TGAATCGTGT AGAAGATTTT CAACAGCATA GTCAGAAACT ACTCTCTGAG 
GAAACGCCTA GTGCTGCGGA GCTGCAGGAC TTGCTAGATG TCAGCTTTGA ATTTGATGTT 
GAACTTCCAC AGCTTGCTGA GATGCGTATC CGTTTGGAAC AAGCCCGTTG GCTAGAAGAG 
GTGCAGCAAG CTTGCCTAGA CCCCAGCTCC CTTACTTTAG ATGATATGAG ACGTCTCATA 
GACCTAGGGG TAGGGCTGGC CCCGTATTCA GCAGTGGAGA AAGCTATGGC CCGGCTGCAG 
GAACTGCTCA CAGTGTCAGA GCACTGGGAC GACAAAGCCA AGAGTCTCCT CAAGGCCAGG 
CCACGACATT CATTGAATAG CCTTGCTACG GCAGTAAAGG AAATCGAAGA GATCCCTGCA 
TATCTGCCCA ATGGTGCGGC TCTGAAAGAC TCAGTGCAGA GAGCCAGAGA CTGGCTTCAG 
GATGTAGAGG GCCTGCAGGC TGGAGGACGT GTGCCAGTGT TAGACACACT CATAGAACTT 
GTTACACGAG GCCGATCTAT CCCCGTACAT CTGAATTCTT TGCCAAGACT GGAAACCCTA 
GTAGCTGAGG TTCAGGCTTG GAAAGAATGT GCTGTTAATA CATTCTTGAC TGAGAATTCT 
CCATATTCTC TCTTAGAGGT GCTGTGTCCT CGATGTGATA TTGGCCTTTT GGGATTGAAA 
AGGAAGCAGA GAAAGTTAAA GGAGCCCTTG CCAAATGGAA AGAAAAAAAG CACCAAATTA 
GAGAGTCTGA GTGACCTGGA GAGAGCTTTA ACTGAAAGCA AGGAGACTGC TTCAGCTATG 
GCAACTCTTG GGGAAGCTCG CCTAAGGGAA ATGGAAGCCT TGCAGTCTCT CAGACTCGCC 
AATGAAGGGA AATTGCTGTC GCCTCTCCAA GATGTGGATA TAAAAATCTG CCTATGTCAG 
AAGGCCCCAG CTGCCCCTAT GATTCAATGT GAACTCTGCA GGGATGCTTT CCACACCAGT 
TGTGTGGCGG TACCCAGTAT TTCACAGGGC CTGCGAATCT GGCTTTGTCC CCATTGTCGG 
AGGTCAGAGA AACCTCCATT AGAGAAAATT CTGCCCCTGC TCGCCTCCCT TCAGCGTATC 
CGAGTTCGCC TTCCTGAGGG AGATGCACTT CGATATATGA TTGAAAGAAC CGTGAACTGG 
CAGCACAGAG CCCAGCAACT GCTTTCGTCA GGGAATCTTA AATTTGTGCA AGATCGAGTG 
GGCTCAGGAC TGTTATATAG CAGATGGCAA GCCTCAGCAG GACAGGTGTC AGACACAAAC 
AAGGTATCTC AACCTCCTGG CACAACATCA TTTTCTTTGC CTGATGACTG GGACAACAGA 
ACCTCATATT TGCACTCCCC CTTCTCAACT GGACGAAGTT GTATCCCCCT CCATGGTGTT 
AGTCCAGAAG TGAATGAACT ATTGATGGAA GCCCAGCTGC TCCAGGTATC CCTTCCTGAA 
ATTCAGGAAC TTTACCAGAC TTTACTTGCA AAGCCAAGCC CTGCTCAGCA GACTGACCGA 
AGCTCACCAG TGAGACCCAG CAGTGAGAAG AATGACTGTT GCCGAGGGAA GCGAGATGGA 
ATTAACAGTC TTGAGAGAAA ACTGAAGAGA CGCCTGGAAA GAGAGGGCCT CTCCAGTGAG 
CGGTGGGAAC GAGTTAAGAA AATGCGGACC CCCAAAAAGA AGAAAATCAA ACTGAGCCAC 
CCCAAGGACA TGAACAATTT CAAGTTAGAG AGAGAGCGTA GCTATGAATT AGTTCGTTCT 
GCTGAAACTC ATTCCCTGCC CTCAGACACA TCCTATTCCG AACAGGAAGA CTCTGAGGAT 
GAAGATGCCA TCTGCCCAGC TGTGAGCTGC CTGCAGCCAG AAGGAGATGA GGTGGACTGG 
GTCCAGTGTG ATGGCAGCTG CAATCAGTGG TTTCATCAGG TCTGTGTTGG TGTCTCCCCA 
GAGATGGCAG AGAAAGAAGA CTACATCTGT GTGCGCTGTA CTGTGAAGGA CGCACCAAGC 
CGAAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM5B ( XM_011509090.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011507392.1 Homo sapiens lysine demethylase 5B (KDM5B), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu64359
Clone ID Related Accession (Same CDS sequence)	XM_011509090.2 , XM_011509091.2 , XM_011509090.3
Accession Version	XM_011509090.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4269bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	lysine-specific demethylase 5B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011509090.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGTTTG CTCCTGGCAA AGCAGTGGGC TCACATATCA GAGGGCATTA TGAACGAATT 
CTCAACCCCT ACAACTTATT CCTGTCCGGA GACAGCCTAA GGTGTTTGCA GAAGCCAAAC 
CTGACCACAG ACACTAAGGA CAAGGAGTAC AAACCCCATG ATATTCCCCA GAGGCAGTCT 
GTGCAGCCTT CGGAAACGTG CCCCCCAGCC CGACGAGCAA AACGCATGAG AGCAGAGAGA 
CAGAGTCTCG CTGTGTTGCC CAGGCTGGAG TGCAGTGGCG CGATCTTGGC TCACTGCAAC 
CTCCGCCTCC TAGATTCCAG CAATTCTTCT GCCTCAGCCT CCCAAGCCAT GAATATTAAA 
ATAGAACCCG AGGAGACAAC GGAAGCCAGA ACTCATAATC TGAGACGTCG AATGGGTTGT 
CCAACTCCAA AATGTGAAAA TGAGAAAGAA ATGAAGAGTA GCATCAAGCA AGAACCTATT 
GAGAGGAAAG ATTATATTGT AGAAAATGAG AAGGAAAAGC CCAAGAGTCG ATCTAAAAAA 
GCCACCAATG CTGTGGACCT GTATGTCTGT CTTTTATGTG GCAGTGGCAA TGATGAAGAC 
CGGCTACTGT TGTGTGATGG CTGTGATGAC AGTTACCATA CCTTTTGCTT GATCCCACCT 
CTCCATGATG TTCCCAAGGG AGACTGGAGG TGTCCTAAGT GTTTGGCTCA GGAATGTAGT 
AAGCCACAAG AAGCATTTGG CTTTGAACAA GCAGCCAGGG ACTATACCCT CCGTACTTTT 
GGGGAAATGG CAGATGCGTT CAAATCTGAT TACTTCAACA TGCCAGTCCA TATGGTCCCC 
ACAGAGCTTG TTGAGAAAGA ATTTTGGAGA CTAGTAAGCA CTATTGAGGA GGATGTCACA 
GTGGAATATG GAGCTGACAT TGCCTCAAAG GAATTTGGCA GTGGCTTTCC TGTCCGAGAT 
GGGAAAATCA AACTCTCACC TGAGGAAGAG GAGTATCTTG ATAGTGGCTG GAATTTGAAC 
AACATGCCAG TGATGGAGCA GTCTGTCCTT GCACATATTA CTGCTGATAT ATGTGGCATG 
AAACTTCCTT GGTTGTATGT GGGAATGTGC TTTTCTTCAT TCTGTTGGCA CATTGAAGAC 
CACTGGAGCT ATTCAATTAA CTACTTGCAC TGGGGTGAGC CAAAAACCTG GTATGGAGTC 
CCAGGGTATG CTGCTGAGCA GCTAGAAAAT GTAATGAAGA AACTAGCTCC AGAACTCTTT 
GTGTCCCAGC CGGATCTCCT CCATCAGCTT GTGACCATCA TGAACCCCAA TACCCTGATG 
ACTCATGAAG TGCCTGTTTA CCGAACTAAT CAGTGTGCTG GGGAGTTTGT GATTACATTT 
CCAAGAGCCT ACCACAGTGG TTTTAACCAG GGTTTTAATT TTGCTGAGGC TGTTAACTTC 
TGCACTGTTG ATTGGCTGCC ATTAGGCCGA CAGTGTGTGG AGCATTATCG CTTGCTTCAT 
CGATATTGTG TGTTTTCCCA CGATGAGATG ATCTGCAAGA TGGCTTCCAA GGCTGATGTA 
TTAGATGTTG TAGTGGCTTC AACTGTTCAG AAAGACATGG CCATTATGAT TGAGGATGAG 
AAAGCTTTAA GAGAAACTGT CCGTAAATTG GGAGTGATTG ATTCGGAAAG AATGGATTTT 
GAGCTGTTGC CAGATGATGA ACGTCAGTGT GTAAAATGCA AAACTACATG CTTCATGTCT 
GCCATCTCCT GTTCTTGTAA ACCTGGCCTT CTTGTTTGCC TGCATCATGT AAAAGAATTG 
TGTTCCTGTC CTCCTTACAA ATATAAATTG CGGTATAGGT ACACGCTGGA TGATCTCTAC 
CCTATGATGA ATGCATTGAA GCTTCGAGCA GAATCTTACA ACGAATGGGC CTTGAATGTG 
AATGAAGCTT TGGAGGCAAA GATCAACAAG AAGAAAAGCC TTGTCAGCTT CAAGGCTTTA 
ATTGAAGAAT CTGAAATGAA GAAATTCCCA GACAATGATC TTTTGCGACA CCTTCGCCTA 
GTCACACAGG ATGCAGAGAA GTGTGCCTCT GTTGCGCAGC AGTTGCTTAA TGGCAAAAGG 
CAAACTAGAT ATCGATCTGG TGGAGGGAAA TCCCAAAATC AGTTGACAGT GAATGAGCTC 
CGGCAGTTTG TAACACAGCT GTATGCTCTT CCATGTGTCC TCAGTCAGAC ACCATTACTA 
AAGGATCTCT TGAATCGTGT AGAAGATTTT CAACAGCATA GTCAGAAACT ACTCTCTGAG 
GAAACGCCTA GTGCTGCGGA GCTGCAGGAC TTGCTAGATG TCAGCTTTGA ATTTGATGTT 
GAACTTCCAC AGCTTGCTGA GATGCGTATC CGTTTGGAAC AAGCCCGTTG GCTAGAAGAG 
GTGCAGCAAG CTTGCCTAGA CCCCAGCTCC CTTACTTTAG ATGATATGAG ACGTCTCATA 
GACCTAGGGG TAGGGCTGGC CCCGTATTCA GCAGTGGAGA AAGCTATGGC CCGGCTGCAG 
GAACTGCTCA CAGTGTCAGA GCACTGGGAC GACAAAGCCA AGAGTCTCCT CAAGGCCAGG 
CCACGACATT CATTGAATAG CCTTGCTACG GCAGTAAAGG AAATCGAAGA GATCCCTGCA 
TATCTGCCCA ATGGTGCGGC TCTGAAAGAC TCAGTGCAGA GAGCCAGAGA CTGGCTTCAG 
GATGTAGAGG GCCTGCAGGC TGGAGGACGT GTGCCAGTGT TAGACACACT CATAGAACTT 
GTTACACGAG GCCGATCTAT CCCCGTACAT CTGAATTCTT TGCCAAGACT GGAAACCCTA 
GTAGCTGAGG TTCAGGCTTG GAAAGAATGT GCTGTTAATA CATTCTTGAC TGAGAATTCT 
CCATATTCTC TCTTAGAGGT GCTGTGTCCT CGATGTGATA TTGGCCTTTT GGGATTGAAA 
AGGAAGCAGA GAAAGTTAAA GGAGCCCTTG CCAAATGGAA AGAAAAAAAG CACCAAATTA 
GAGAGTCTGA GTGACCTGGA GAGAGCTTTA ACTGAAAGCA AGGAGACTGC TTCAGCTATG 
GCAACTCTTG GGGAAGCTCG CCTAAGGGAA ATGGAAGCCT TGCAGTCTCT CAGACTCGCC 
AATGAAGGGA AATTGCTGTC GCCTCTCCAA GATGTGGATA TAAAAATCTG CCTATGTCAG 
AAGGCCCCAG CTGCCCCTAT GATTCAATGT GAACTCTGCA GGGATGCTTT CCACACCAGT 
TGTGTGGCGG TACCCAGTAT TTCACAGGGC CTGCGAATCT GGCTTTGTCC CCATTGTCGG 
AGGTCAGAGA AACCTCCATT AGAGAAAATT CTGCCCCTGC TCGCCTCCCT TCAGCGTATC 
CGAGTTCGCC TTCCTGAGGG AGATGCACTT CGATATATGA TTGAAAGAAC CGTGAACTGG 
CAGCACAGAG CCCAGCAACT GCTTTCGTCA GGGAATCTTA AATTTGTGCA AGATCGAGTG 
GGCTCAGGAC TGTTATATAG CAGATGGCAA GCCTCAGCAG GACAGGTGTC AGACACAAAC 
AAGGTATCTC AACCTCCTGG CACAACATCA TTTTCTTTGC CTGATGACTG GGACAACAGA 
ACCTCATATT TGCACTCCCC CTTCTCAACT GGACGAAGTT GTATCCCCCT CCATGGTGTT 
AGTCCAGAAG TGAATGAACT ATTGATGGAA GCCCAGCTGC TCCAGGTATC CCTTCCTGAA 
ATTCAGGAAC TTTACCAGAC TTTACTTGCA AAGCCAAGCC CTGCTCAGCA GACTGACCGA 
AGCTCACCAG TGAGACCCAG CAGTGAGAAG AATGACTGTT GCCGAGGGAA GCGAGATGGA 
ATTAACAGTC TTGAGAGAAA ACTGAAGAGA CGCCTGGAAA GAGAGGGCCT CTCCAGTGAG 
CGGTGGGAAC GAGTTAAGAA AATGCGGACC CCCAAAAAGA AGAAAATCAA ACTGAGCCAC 
CCCAAGGACA TGAACAATTT CAAGTTAGAG AGAGAGCGTA GCTATGAATT AGTTCGTTCT 
GCTGAAACTC ATTCCCTGCC CTCAGACACA TCCTATTCCG AACAGGAAGA CTCTGAGGAT 
GAAGATGCCA TCTGCCCAGC TGTGAGCTGC CTGCAGCCAG AAGGAGATGA GGTGGACTGG 
GTCCAGTGTG ATGGCAGCTG CAATCAGTGG TTTCATCAGG TCTGTGTTGG TGTCTCCCCA 
GAGATGGCAG AGAAAGAAGA CTACATCTGT GTGCGCTGTA CTGTGAAGGA CGCACCAAGC 
CGAAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011507392.1
CDS	643..4911
Translation	MGFAPGKAVGSHIRGHYERILNPYNLFLSGDSLRCLQKPNLTTDTKDKEYKPHDIPQRQSVQPSETCPPARRAKRMRAERQSLAVLPRLECSGAILAHCNLRLLDSSNSSASASQAMNIKIEPEETTEARTHNLRRRMGCPTPKCENEKEMKSSIKQEPIERKDYIVENEKEKPKSRSKKATNAVDLYVCLLCGSGNDEDRLLLCDGCDDSYHTFCLIPPLHDVPKGDWRCPKCLAQECSKPQEAFGFEQAARDYTLRTFGEMADAFKSDYFNMPVHMVPTELVEKEFWRLVSTIEEDVTVEYGADIASKEFGSGFPVRDGKIKLSPEEEEYLDSGWNLNNMPVMEQSVLAHITADICGMKLPWLYVGMCFSSFCWHIEDHWSYSINYLHWGEPKTWYGVPGYAAEQLENVMKKLAPELFVSQPDLLHQLVTIMNPNTLMTHEVPVYRTNQCAGEFVITFPRAYHSGFNQGFNFAEAVNFCTVDWLPLGRQCVEHYRLLHRYCVFSHDEMICKMASKADVLDVVVASTVQKDMAIMIEDEKALRETVRKLGVIDSERMDFELLPDDERQCVKCKTTCFMSAISCSCKPGLLVCLHHVKELCSCPPYKYKLRYRYTLDDLYPMMNALKLRAESYNEWALNVNEALEAKINKKKSLVSFKALIEESEMKKFPDNDLLRHLRLVTQDAEKCASVAQQLLNGKRQTRYRSGGGKSQNQLTVNELRQFVTQLYALPCVLSQTPLLKDLLNRVEDFQQHSQKLLSEETPSAAELQDLLDVSFEFDVELPQLAEMRIRLEQARWLEEVQQACLDPSSLTLDDMRRLIDLGVGLAPYSAVEKAMARLQELLTVSEHWDDKAKSLLKARPRHSLNSLATAVKEIEEIPAYLPNGAALKDSVQRARDWLQDVEGLQAGGRVPVLDTLIELVTRGRSIPVHLNSLPRLETLVAEVQAWKECAVNTFLTENSPYSLLEVLCPRCDIGLLGLKRKQRKLKEPLPNGKKKSTKLESLSDLERALTESKETASAMATLGEARLREMEALQSLRLANEGKLLSPLQDVDIKICLCQKAPAAPMIQCELCRDAFHTSCVAVPSISQGLRIWLCPHCRRSEKPPLEKILPLLASLQRIRVRLPEGDALRYMIERTVNWQHRAQQLLSSGNLKFVQDRVGSGLLYSRWQASAGQVSDTNKVSQPPGTTSFSLPDDWDNRTSYLHSPFSTGRSCIPLHGVSPEVNELLMEAQLLQVSLPEIQELYQTLLAKPSPAQQTDRSSPVRPSSEKNDCCRGKRDGINSLERKLKRRLEREGLSSERWERVKKMRTPKKKKIKLSHPKDMNNFKLERERSYELVRSAETHSLPSDTSYSEQEDSEDEDAICPAVSCLQPEGDEVDWVQCDGSCNQWFHQVCVGVSPEMAEKEDYICVRCTVKDAPSRK

Target ORF information:

RefSeq Version	XM_011509090.3
Organism	Homo sapiens(human)
Definition	Homo sapiens lysine demethylase 5B (KDM5B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011509090.3

ORF Insert Sequence:

ATGGGGTTTG CTCCTGGCAA AGCAGTGGGC TCACATATCA GAGGGCATTA TGAACGAATT 
CTCAACCCCT ACAACTTATT CCTGTCCGGA GACAGCCTAA GGTGTTTGCA GAAGCCAAAC 
CTGACCACAG ACACTAAGGA CAAGGAGTAC AAACCCCATG ATATTCCCCA GAGGCAGTCT 
GTGCAGCCTT CGGAAACGTG CCCCCCAGCC CGACGAGCAA AACGCATGAG AGCAGAGAGA 
CAGAGTCTCG CTGTGTTGCC CAGGCTGGAG TGCAGTGGCG CGATCTTGGC TCACTGCAAC 
CTCCGCCTCC TAGATTCCAG CAATTCTTCT GCCTCAGCCT CCCAAGCCAT GAATATTAAA 
ATAGAACCCG AGGAGACAAC GGAAGCCAGA ACTCATAATC TGAGACGTCG AATGGGTTGT 
CCAACTCCAA AATGTGAAAA TGAGAAAGAA ATGAAGAGTA GCATCAAGCA AGAACCTATT 
GAGAGGAAAG ATTATATTGT AGAAAATGAG AAGGAAAAGC CCAAGAGTCG ATCTAAAAAA 
GCCACCAATG CTGTGGACCT GTATGTCTGT CTTTTATGTG GCAGTGGCAA TGATGAAGAC 
CGGCTACTGT TGTGTGATGG CTGTGATGAC AGTTACCATA CCTTTTGCTT GATCCCACCT 
CTCCATGATG TTCCCAAGGG AGACTGGAGG TGTCCTAAGT GTTTGGCTCA GGAATGTAGT 
AAGCCACAAG AAGCATTTGG CTTTGAACAA GCAGCCAGGG ACTATACCCT CCGTACTTTT 
GGGGAAATGG CAGATGCGTT CAAATCTGAT TACTTCAACA TGCCAGTCCA TATGGTCCCC 
ACAGAGCTTG TTGAGAAAGA ATTTTGGAGA CTAGTAAGCA CTATTGAGGA GGATGTCACA 
GTGGAATATG GAGCTGACAT TGCCTCAAAG GAATTTGGCA GTGGCTTTCC TGTCCGAGAT 
GGGAAAATCA AACTCTCACC TGAGGAAGAG GAGTATCTTG ATAGTGGCTG GAATTTGAAC 
AACATGCCAG TGATGGAGCA GTCTGTCCTT GCACATATTA CTGCTGATAT ATGTGGCATG 
AAACTTCCTT GGTTGTATGT GGGAATGTGC TTTTCTTCAT TCTGTTGGCA CATTGAAGAC 
CACTGGAGCT ATTCAATTAA CTACTTGCAC TGGGGTGAGC CAAAAACCTG GTATGGAGTC 
CCAGGGTATG CTGCTGAGCA GCTAGAAAAT GTAATGAAGA AACTAGCTCC AGAACTCTTT 
GTGTCCCAGC CGGATCTCCT CCATCAGCTT GTGACCATCA TGAACCCCAA TACCCTGATG 
ACTCATGAAG TGCCTGTTTA CCGAACTAAT CAGTGTGCTG GGGAGTTTGT GATTACATTT 
CCAAGAGCCT ACCACAGTGG TTTTAACCAG GGTTTTAATT TTGCTGAGGC TGTTAACTTC 
TGCACTGTTG ATTGGCTGCC ATTAGGCCGA CAGTGTGTGG AGCATTATCG CTTGCTTCAT 
CGATATTGTG TGTTTTCCCA CGATGAGATG ATCTGCAAGA TGGCTTCCAA GGCTGATGTA 
TTAGATGTTG TAGTGGCTTC AACTGTTCAG AAAGACATGG CCATTATGAT TGAGGATGAG 
AAAGCTTTAA GAGAAACTGT CCGTAAATTG GGAGTGATTG ATTCGGAAAG AATGGATTTT 
GAGCTGTTGC CAGATGATGA ACGTCAGTGT GTAAAATGCA AAACTACATG CTTCATGTCT 
GCCATCTCCT GTTCTTGTAA ACCTGGCCTT CTTGTTTGCC TGCATCATGT AAAAGAATTG 
TGTTCCTGTC CTCCTTACAA ATATAAATTG CGGTATAGGT ACACGCTGGA TGATCTCTAC 
CCTATGATGA ATGCATTGAA GCTTCGAGCA GAATCTTACA ACGAATGGGC CTTGAATGTG 
AATGAAGCTT TGGAGGCAAA GATCAACAAG AAGAAAAGCC TTGTCAGCTT CAAGGCTTTA 
ATTGAAGAAT CTGAAATGAA GAAATTCCCA GACAATGATC TTTTGCGACA CCTTCGCCTA 
GTCACACAGG ATGCAGAGAA GTGTGCCTCT GTTGCGCAGC AGTTGCTTAA TGGCAAAAGG 
CAAACTAGAT ATCGATCTGG TGGAGGGAAA TCCCAAAATC AGTTGACAGT GAATGAGCTC 
CGGCAGTTTG TAACACAGCT GTATGCTCTT CCATGTGTCC TCAGTCAGAC ACCATTACTA 
AAGGATCTCT TGAATCGTGT AGAAGATTTT CAACAGCATA GTCAGAAACT ACTCTCTGAG 
GAAACGCCTA GTGCTGCGGA GCTGCAGGAC TTGCTAGATG TCAGCTTTGA ATTTGATGTT 
GAACTTCCAC AGCTTGCTGA GATGCGTATC CGTTTGGAAC AAGCCCGTTG GCTAGAAGAG 
GTGCAGCAAG CTTGCCTAGA CCCCAGCTCC CTTACTTTAG ATGATATGAG ACGTCTCATA 
GACCTAGGGG TAGGGCTGGC CCCGTATTCA GCAGTGGAGA AAGCTATGGC CCGGCTGCAG 
GAACTGCTCA CAGTGTCAGA GCACTGGGAC GACAAAGCCA AGAGTCTCCT CAAGGCCAGG 
CCACGACATT CATTGAATAG CCTTGCTACG GCAGTAAAGG AAATCGAAGA GATCCCTGCA 
TATCTGCCCA ATGGTGCGGC TCTGAAAGAC TCAGTGCAGA GAGCCAGAGA CTGGCTTCAG 
GATGTAGAGG GCCTGCAGGC TGGAGGACGT GTGCCAGTGT TAGACACACT CATAGAACTT 
GTTACACGAG GCCGATCTAT CCCCGTACAT CTGAATTCTT TGCCAAGACT GGAAACCCTA 
GTAGCTGAGG TTCAGGCTTG GAAAGAATGT GCTGTTAATA CATTCTTGAC TGAGAATTCT 
CCATATTCTC TCTTAGAGGT GCTGTGTCCT CGATGTGATA TTGGCCTTTT GGGATTGAAA 
AGGAAGCAGA GAAAGTTAAA GGAGCCCTTG CCAAATGGAA AGAAAAAAAG CACCAAATTA 
GAGAGTCTGA GTGACCTGGA GAGAGCTTTA ACTGAAAGCA AGGAGACTGC TTCAGCTATG 
GCAACTCTTG GGGAAGCTCG CCTAAGGGAA ATGGAAGCCT TGCAGTCTCT CAGACTCGCC 
AATGAAGGGA AATTGCTGTC GCCTCTCCAA GATGTGGATA TAAAAATCTG CCTATGTCAG 
AAGGCCCCAG CTGCCCCTAT GATTCAATGT GAACTCTGCA GGGATGCTTT CCACACCAGT 
TGTGTGGCGG TACCCAGTAT TTCACAGGGC CTGCGAATCT GGCTTTGTCC CCATTGTCGG 
AGGTCAGAGA AACCTCCATT AGAGAAAATT CTGCCCCTGC TCGCCTCCCT TCAGCGTATC 
CGAGTTCGCC TTCCTGAGGG AGATGCACTT CGATATATGA TTGAAAGAAC CGTGAACTGG 
CAGCACAGAG CCCAGCAACT GCTTTCGTCA GGGAATCTTA AATTTGTGCA AGATCGAGTG 
GGCTCAGGAC TGTTATATAG CAGATGGCAA GCCTCAGCAG GACAGGTGTC AGACACAAAC 
AAGGTATCTC AACCTCCTGG CACAACATCA TTTTCTTTGC CTGATGACTG GGACAACAGA 
ACCTCATATT TGCACTCCCC CTTCTCAACT GGACGAAGTT GTATCCCCCT CCATGGTGTT 
AGTCCAGAAG TGAATGAACT ATTGATGGAA GCCCAGCTGC TCCAGGTATC CCTTCCTGAA 
ATTCAGGAAC TTTACCAGAC TTTACTTGCA AAGCCAAGCC CTGCTCAGCA GACTGACCGA 
AGCTCACCAG TGAGACCCAG CAGTGAGAAG AATGACTGTT GCCGAGGGAA GCGAGATGGA 
ATTAACAGTC TTGAGAGAAA ACTGAAGAGA CGCCTGGAAA GAGAGGGCCT CTCCAGTGAG 
CGGTGGGAAC GAGTTAAGAA AATGCGGACC CCCAAAAAGA AGAAAATCAA ACTGAGCCAC 
CCCAAGGACA TGAACAATTT CAAGTTAGAG AGAGAGCGTA GCTATGAATT AGTTCGTTCT 
GCTGAAACTC ATTCCCTGCC CTCAGACACA TCCTATTCCG AACAGGAAGA CTCTGAGGAT 
GAAGATGCCA TCTGCCCAGC TGTGAGCTGC CTGCAGCCAG AAGGAGATGA GGTGGACTGG 
GTCCAGTGTG ATGGCAGCTG CAATCAGTGG TTTCATCAGG TCTGTGTTGG TGTCTCCCCA 
GAGATGGCAG AGAAAGAAGA CTACATCTGT GTGCGCTGTA CTGTGAAGGA CGCACCAAGC 
CGAAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM5B ( XM_011509092.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011507394.1 Homo sapiens lysine demethylase 5B (KDM5B), transcript variant X3, mRNA.

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CloneID	OHu64360
Clone ID Related Accession (Same CDS sequence)	XM_011509092.2
Accession Version	XM_011509092.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4161bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	lysine-specific demethylase 5B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011509092.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGTTTG CTCCTGGCAA AGCAGTGGGC TCACATATCA GAGGGCATTA TGAACGAATT 
CTCAACCCCT ACAACTTATT CCTGTCCGGA GACAGCCTAA GGTGTTTGCA GAAGCCAAAC 
CTGACCACAG ACACTAAGGA CAAGGAGTAC AAACCCCATG ATATTCCCCA GAGGCAGTCT 
GTGCAGCCTT CGGAAACGTG CCCCCCAGCC CGACGAGCAA AACGCATGAG AGCAGAGGCC 
ATGAATATTA AAATAGAACC CGAGGAGACA ACGGAAGCCA GAACTCATAA TCTGAGACGT 
CGAATGGGTT GTCCAACTCC AAAATGTGAA AATGAGAAAG AAATGAAGAG TAGCATCAAG 
CAAGAACCTA TTGAGAGGAA AGATTATATT GTAGAAAATG AGAAGGAAAA GCCCAAGAGT 
CGATCTAAAA AAGCCACCAA TGCTGTGGAC CTGTATGTCT GTCTTTTATG TGGCAGTGGC 
AATGATGAAG ACCGGCTACT GTTGTGTGAT GGCTGTGATG ACAGTTACCA TACCTTTTGC 
TTGATCCCAC CTCTCCATGA TGTTCCCAAG GGAGACTGGA GGTGTCCTAA GTGTTTGGCT 
CAGGAATGTA GTAAGCCACA AGAAGCATTT GGCTTTGAAC AAGCAGCCAG GGACTATACC 
CTCCGTACTT TTGGGGAAAT GGCAGATGCG TTCAAATCTG ATTACTTCAA CATGCCAGTC 
CATATGGTCC CCACAGAGCT TGTTGAGAAA GAATTTTGGA GACTAGTAAG CACTATTGAG 
GAGGATGTCA CAGTGGAATA TGGAGCTGAC ATTGCCTCAA AGGAATTTGG CAGTGGCTTT 
CCTGTCCGAG ATGGGAAAAT CAAACTCTCA CCTGAGGAAG AGGAGTATCT TGATAGTGGC 
TGGAATTTGA ACAACATGCC AGTGATGGAG CAGTCTGTCC TTGCACATAT TACTGCTGAT 
ATATGTGGCA TGAAACTTCC TTGGTTGTAT GTGGGAATGT GCTTTTCTTC ATTCTGTTGG 
CACATTGAAG ACCACTGGAG CTATTCAATT AACTACTTGC ACTGGGGTGA GCCAAAAACC 
TGGTATGGAG TCCCAGGGTA TGCTGCTGAG CAGCTAGAAA ATGTAATGAA GAAACTAGCT 
CCAGAACTCT TTGTGTCCCA GCCGGATCTC CTCCATCAGC TTGTGACCAT CATGAACCCC 
AATACCCTGA TGACTCATGA AGTGCCTGTT TACCGAACTA ATCAGTGTGC TGGGGAGTTT 
GTGATTACAT TTCCAAGAGC CTACCACAGT GGTTTTAACC AGGGTTTTAA TTTTGCTGAG 
GCTGTTAACT TCTGCACTGT TGATTGGCTG CCATTAGGCC GACAGTGTGT GGAGCATTAT 
CGCTTGCTTC ATCGATATTG TGTGTTTTCC CACGATGAGA TGATCTGCAA GATGGCTTCC 
AAGGCTGATG TATTAGATGT TGTAGTGGCT TCAACTGTTC AGAAAGACAT GGCCATTATG 
ATTGAGGATG AGAAAGCTTT AAGAGAAACT GTCCGTAAAT TGGGAGTGAT TGATTCGGAA 
AGAATGGATT TTGAGCTGTT GCCAGATGAT GAACGTCAGT GTGTAAAATG CAAAACTACA 
TGCTTCATGT CTGCCATCTC CTGTTCTTGT AAACCTGGCC TTCTTGTTTG CCTGCATCAT 
GTAAAAGAAT TGTGTTCCTG TCCTCCTTAC AAATATAAAT TGCGGTATAG GTACACGCTG 
GATGATCTCT ACCCTATGAT GAATGCATTG AAGCTTCGAG CAGAATCTTA CAACGAATGG 
GCCTTGAATG TGAATGAAGC TTTGGAGGCA AAGATCAACA AGAAGAAAAG CCTTGTCAGC 
TTCAAGGCTT TAATTGAAGA ATCTGAAATG AAGAAATTCC CAGACAATGA TCTTTTGCGA 
CACCTTCGCC TAGTCACACA GGATGCAGAG AAGTGTGCCT CTGTTGCGCA GCAGTTGCTT 
AATGGCAAAA GGCAAACTAG ATATCGATCT GGTGGAGGGA AATCCCAAAA TCAGTTGACA 
GTGAATGAGC TCCGGCAGTT TGTAACACAG CTGTATGCTC TTCCATGTGT CCTCAGTCAG 
ACACCATTAC TAAAGGATCT CTTGAATCGT GTAGAAGATT TTCAACAGCA TAGTCAGAAA 
CTACTCTCTG AGGAAACGCC TAGTGCTGCG GAGCTGCAGG ACTTGCTAGA TGTCAGCTTT 
GAATTTGATG TTGAACTTCC ACAGCTTGCT GAGATGCGTA TCCGTTTGGA ACAAGCCCGT 
TGGCTAGAAG AGGTGCAGCA AGCTTGCCTA GACCCCAGCT CCCTTACTTT AGATGATATG 
AGACGTCTCA TAGACCTAGG GGTAGGGCTG GCCCCGTATT CAGCAGTGGA GAAAGCTATG 
GCCCGGCTGC AGGAACTGCT CACAGTGTCA GAGCACTGGG ACGACAAAGC CAAGAGTCTC 
CTCAAGGCCA GGCCACGACA TTCATTGAAT AGCCTTGCTA CGGCAGTAAA GGAAATCGAA 
GAGATCCCTG CATATCTGCC CAATGGTGCG GCTCTGAAAG ACTCAGTGCA GAGAGCCAGA 
GACTGGCTTC AGGATGTAGA GGGCCTGCAG GCTGGAGGAC GTGTGCCAGT GTTAGACACA 
CTCATAGAAC TTGTTACACG AGGCCGATCT ATCCCCGTAC ATCTGAATTC TTTGCCAAGA 
CTGGAAACCC TAGTAGCTGA GGTTCAGGCT TGGAAAGAAT GTGCTGTTAA TACATTCTTG 
ACTGAGAATT CTCCATATTC TCTCTTAGAG GTGCTGTGTC CTCGATGTGA TATTGGCCTT 
TTGGGATTGA AAAGGAAGCA GAGAAAGTTA AAGGAGCCCT TGCCAAATGG AAAGAAAAAA 
AGCACCAAAT TAGAGAGTCT GAGTGACCTG GAGAGAGCTT TAACTGAAAG CAAGGAGACT 
GCTTCAGCTA TGGCAACTCT TGGGGAAGCT CGCCTAAGGG AAATGGAAGC CTTGCAGTCT 
CTCAGACTCG CCAATGAAGG GAAATTGCTG TCGCCTCTCC AAGATGTGGA TATAAAAATC 
TGCCTATGTC AGAAGGCCCC AGCTGCCCCT ATGATTCAAT GTGAACTCTG CAGGGATGCT 
TTCCACACCA GTTGTGTGGC GGTACCCAGT ATTTCACAGG GCCTGCGAAT CTGGCTTTGT 
CCCCATTGTC GGAGGTCAGA GAAACCTCCA TTAGAGAAAA TTCTGCCCCT GCTCGCCTCC 
CTTCAGCGTA TCCGAGTTCG CCTTCCTGAG GGAGATGCAC TTCGATATAT GATTGAAAGA 
ACCGTGAACT GGCAGCACAG AGCCCAGCAA CTGCTTTCGT CAGGGAATCT TAAATTTGTG 
CAAGATCGAG TGGGCTCAGG ACTGTTATAT AGCAGATGGC AAGCCTCAGC AGGACAGGTG 
TCAGACACAA ACAAGGTATC TCAACCTCCT GGCACAACAT CATTTTCTTT GCCTGATGAC 
TGGGACAACA GAACCTCATA TTTGCACTCC CCCTTCTCAA CTGGACGAAG TTGTATCCCC 
CTCCATGGTG TTAGTCCAGA AGTGAATGAA CTATTGATGG AAGCCCAGCT GCTCCAGGTA 
TCCCTTCCTG AAATTCAGGA ACTTTACCAG ACTTTACTTG CAAAGCCAAG CCCTGCTCAG 
CAGACTGACC GAAGCTCACC AGTGAGACCC AGCAGTGAGA AGAATGACTG TTGCCGAGGG 
AAGCGAGATG GAATTAACAG TCTTGAGAGA AAACTGAAGA GACGCCTGGA AAGAGAGGGC 
CTCTCCAGTG AGCGGTGGGA ACGAGTTAAG AAAATGCGGA CCCCCAAAAA GAAGAAAATC 
AAACTGAGCC ACCCCAAGGA CATGAACAAT TTCAAGTTAG AGAGAGAGCG TAGCTATGAA 
TTAGTTCGTT CTGCTGAAAC TCATTCCCTG CCCTCAGACA CATCCTATTC CGAACAGGAA 
GACTCTGAGG ATGAAGATGC CATCTGCCCA GCTGTGAGCT GCCTGCAGCC AGAAGGAGAT 
GAGGTGGACT GGGTCCAGTG TGATGGCAGC TGCAATCAGT GGTTTCATCA GGTCTGTGTT 
GGTGTCTCCC CAGAGATGGC AGAGAAAGAA GACTACATCT GTGTGCGCTG TACTGTGAAG 
GACGCACCAA GCCGAAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011507394.1
CDS	583..4743
Translation	MGFAPGKAVGSHIRGHYERILNPYNLFLSGDSLRCLQKPNLTTDTKDKEYKPHDIPQRQSVQPSETCPPARRAKRMRAEAMNIKIEPEETTEARTHNLRRRMGCPTPKCENEKEMKSSIKQEPIERKDYIVENEKEKPKSRSKKATNAVDLYVCLLCGSGNDEDRLLLCDGCDDSYHTFCLIPPLHDVPKGDWRCPKCLAQECSKPQEAFGFEQAARDYTLRTFGEMADAFKSDYFNMPVHMVPTELVEKEFWRLVSTIEEDVTVEYGADIASKEFGSGFPVRDGKIKLSPEEEEYLDSGWNLNNMPVMEQSVLAHITADICGMKLPWLYVGMCFSSFCWHIEDHWSYSINYLHWGEPKTWYGVPGYAAEQLENVMKKLAPELFVSQPDLLHQLVTIMNPNTLMTHEVPVYRTNQCAGEFVITFPRAYHSGFNQGFNFAEAVNFCTVDWLPLGRQCVEHYRLLHRYCVFSHDEMICKMASKADVLDVVVASTVQKDMAIMIEDEKALRETVRKLGVIDSERMDFELLPDDERQCVKCKTTCFMSAISCSCKPGLLVCLHHVKELCSCPPYKYKLRYRYTLDDLYPMMNALKLRAESYNEWALNVNEALEAKINKKKSLVSFKALIEESEMKKFPDNDLLRHLRLVTQDAEKCASVAQQLLNGKRQTRYRSGGGKSQNQLTVNELRQFVTQLYALPCVLSQTPLLKDLLNRVEDFQQHSQKLLSEETPSAAELQDLLDVSFEFDVELPQLAEMRIRLEQARWLEEVQQACLDPSSLTLDDMRRLIDLGVGLAPYSAVEKAMARLQELLTVSEHWDDKAKSLLKARPRHSLNSLATAVKEIEEIPAYLPNGAALKDSVQRARDWLQDVEGLQAGGRVPVLDTLIELVTRGRSIPVHLNSLPRLETLVAEVQAWKECAVNTFLTENSPYSLLEVLCPRCDIGLLGLKRKQRKLKEPLPNGKKKSTKLESLSDLERALTESKETASAMATLGEARLREMEALQSLRLANEGKLLSPLQDVDIKICLCQKAPAAPMIQCELCRDAFHTSCVAVPSISQGLRIWLCPHCRRSEKPPLEKILPLLASLQRIRVRLPEGDALRYMIERTVNWQHRAQQLLSSGNLKFVQDRVGSGLLYSRWQASAGQVSDTNKVSQPPGTTSFSLPDDWDNRTSYLHSPFSTGRSCIPLHGVSPEVNELLMEAQLLQVSLPEIQELYQTLLAKPSPAQQTDRSSPVRPSSEKNDCCRGKRDGINSLERKLKRRLEREGLSSERWERVKKMRTPKKKKIKLSHPKDMNNFKLERERSYELVRSAETHSLPSDTSYSEQEDSEDEDAICPAVSCLQPEGDEVDWVQCDGSCNQWFHQVCVGVSPEMAEKEDYICVRCTVKDAPSRK

Target ORF information:

RefSeq Version	XM_011509092.2
Organism	Homo sapiens(human)
Definition	Homo sapiens lysine demethylase 5B (KDM5B), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011509092.2

ORF Insert Sequence:

ATGGGGTTTG CTCCTGGCAA AGCAGTGGGC TCACATATCA GAGGGCATTA TGAACGAATT 
CTCAACCCCT ACAACTTATT CCTGTCCGGA GACAGCCTAA GGTGTTTGCA GAAGCCAAAC 
CTGACCACAG ACACTAAGGA CAAGGAGTAC AAACCCCATG ATATTCCCCA GAGGCAGTCT 
GTGCAGCCTT CGGAAACGTG CCCCCCAGCC CGACGAGCAA AACGCATGAG AGCAGAGGCC 
ATGAATATTA AAATAGAACC CGAGGAGACA ACGGAAGCCA GAACTCATAA TCTGAGACGT 
CGAATGGGTT GTCCAACTCC AAAATGTGAA AATGAGAAAG AAATGAAGAG TAGCATCAAG 
CAAGAACCTA TTGAGAGGAA AGATTATATT GTAGAAAATG AGAAGGAAAA GCCCAAGAGT 
CGATCTAAAA AAGCCACCAA TGCTGTGGAC CTGTATGTCT GTCTTTTATG TGGCAGTGGC 
AATGATGAAG ACCGGCTACT GTTGTGTGAT GGCTGTGATG ACAGTTACCA TACCTTTTGC 
TTGATCCCAC CTCTCCATGA TGTTCCCAAG GGAGACTGGA GGTGTCCTAA GTGTTTGGCT 
CAGGAATGTA GTAAGCCACA AGAAGCATTT GGCTTTGAAC AAGCAGCCAG GGACTATACC 
CTCCGTACTT TTGGGGAAAT GGCAGATGCG TTCAAATCTG ATTACTTCAA CATGCCAGTC 
CATATGGTCC CCACAGAGCT TGTTGAGAAA GAATTTTGGA GACTAGTAAG CACTATTGAG 
GAGGATGTCA CAGTGGAATA TGGAGCTGAC ATTGCCTCAA AGGAATTTGG CAGTGGCTTT 
CCTGTCCGAG ATGGGAAAAT CAAACTCTCA CCTGAGGAAG AGGAGTATCT TGATAGTGGC 
TGGAATTTGA ACAACATGCC AGTGATGGAG CAGTCTGTCC TTGCACATAT TACTGCTGAT 
ATATGTGGCA TGAAACTTCC TTGGTTGTAT GTGGGAATGT GCTTTTCTTC ATTCTGTTGG 
CACATTGAAG ACCACTGGAG CTATTCAATT AACTACTTGC ACTGGGGTGA GCCAAAAACC 
TGGTATGGAG TCCCAGGGTA TGCTGCTGAG CAGCTAGAAA ATGTAATGAA GAAACTAGCT 
CCAGAACTCT TTGTGTCCCA GCCGGATCTC CTCCATCAGC TTGTGACCAT CATGAACCCC 
AATACCCTGA TGACTCATGA AGTGCCTGTT TACCGAACTA ATCAGTGTGC TGGGGAGTTT 
GTGATTACAT TTCCAAGAGC CTACCACAGT GGTTTTAACC AGGGTTTTAA TTTTGCTGAG 
GCTGTTAACT TCTGCACTGT TGATTGGCTG CCATTAGGCC GACAGTGTGT GGAGCATTAT 
CGCTTGCTTC ATCGATATTG TGTGTTTTCC CACGATGAGA TGATCTGCAA GATGGCTTCC 
AAGGCTGATG TATTAGATGT TGTAGTGGCT TCAACTGTTC AGAAAGACAT GGCCATTATG 
ATTGAGGATG AGAAAGCTTT AAGAGAAACT GTCCGTAAAT TGGGAGTGAT TGATTCGGAA 
AGAATGGATT TTGAGCTGTT GCCAGATGAT GAACGTCAGT GTGTAAAATG CAAAACTACA 
TGCTTCATGT CTGCCATCTC CTGTTCTTGT AAACCTGGCC TTCTTGTTTG CCTGCATCAT 
GTAAAAGAAT TGTGTTCCTG TCCTCCTTAC AAATATAAAT TGCGGTATAG GTACACGCTG 
GATGATCTCT ACCCTATGAT GAATGCATTG AAGCTTCGAG CAGAATCTTA CAACGAATGG 
GCCTTGAATG TGAATGAAGC TTTGGAGGCA AAGATCAACA AGAAGAAAAG CCTTGTCAGC 
TTCAAGGCTT TAATTGAAGA ATCTGAAATG AAGAAATTCC CAGACAATGA TCTTTTGCGA 
CACCTTCGCC TAGTCACACA GGATGCAGAG AAGTGTGCCT CTGTTGCGCA GCAGTTGCTT 
AATGGCAAAA GGCAAACTAG ATATCGATCT GGTGGAGGGA AATCCCAAAA TCAGTTGACA 
GTGAATGAGC TCCGGCAGTT TGTAACACAG CTGTATGCTC TTCCATGTGT CCTCAGTCAG 
ACACCATTAC TAAAGGATCT CTTGAATCGT GTAGAAGATT TTCAACAGCA TAGTCAGAAA 
CTACTCTCTG AGGAAACGCC TAGTGCTGCG GAGCTGCAGG ACTTGCTAGA TGTCAGCTTT 
GAATTTGATG TTGAACTTCC ACAGCTTGCT GAGATGCGTA TCCGTTTGGA ACAAGCCCGT 
TGGCTAGAAG AGGTGCAGCA AGCTTGCCTA GACCCCAGCT CCCTTACTTT AGATGATATG 
AGACGTCTCA TAGACCTAGG GGTAGGGCTG GCCCCGTATT CAGCAGTGGA GAAAGCTATG 
GCCCGGCTGC AGGAACTGCT CACAGTGTCA GAGCACTGGG ACGACAAAGC CAAGAGTCTC 
CTCAAGGCCA GGCCACGACA TTCATTGAAT AGCCTTGCTA CGGCAGTAAA GGAAATCGAA 
GAGATCCCTG CATATCTGCC CAATGGTGCG GCTCTGAAAG ACTCAGTGCA GAGAGCCAGA 
GACTGGCTTC AGGATGTAGA GGGCCTGCAG GCTGGAGGAC GTGTGCCAGT GTTAGACACA 
CTCATAGAAC TTGTTACACG AGGCCGATCT ATCCCCGTAC ATCTGAATTC TTTGCCAAGA 
CTGGAAACCC TAGTAGCTGA GGTTCAGGCT TGGAAAGAAT GTGCTGTTAA TACATTCTTG 
ACTGAGAATT CTCCATATTC TCTCTTAGAG GTGCTGTGTC CTCGATGTGA TATTGGCCTT 
TTGGGATTGA AAAGGAAGCA GAGAAAGTTA AAGGAGCCCT TGCCAAATGG AAAGAAAAAA 
AGCACCAAAT TAGAGAGTCT GAGTGACCTG GAGAGAGCTT TAACTGAAAG CAAGGAGACT 
GCTTCAGCTA TGGCAACTCT TGGGGAAGCT CGCCTAAGGG AAATGGAAGC CTTGCAGTCT 
CTCAGACTCG CCAATGAAGG GAAATTGCTG TCGCCTCTCC AAGATGTGGA TATAAAAATC 
TGCCTATGTC AGAAGGCCCC AGCTGCCCCT ATGATTCAAT GTGAACTCTG CAGGGATGCT 
TTCCACACCA GTTGTGTGGC GGTACCCAGT ATTTCACAGG GCCTGCGAAT CTGGCTTTGT 
CCCCATTGTC GGAGGTCAGA GAAACCTCCA TTAGAGAAAA TTCTGCCCCT GCTCGCCTCC 
CTTCAGCGTA TCCGAGTTCG CCTTCCTGAG GGAGATGCAC TTCGATATAT GATTGAAAGA 
ACCGTGAACT GGCAGCACAG AGCCCAGCAA CTGCTTTCGT CAGGGAATCT TAAATTTGTG 
CAAGATCGAG TGGGCTCAGG ACTGTTATAT AGCAGATGGC AAGCCTCAGC AGGACAGGTG 
TCAGACACAA ACAAGGTATC TCAACCTCCT GGCACAACAT CATTTTCTTT GCCTGATGAC 
TGGGACAACA GAACCTCATA TTTGCACTCC CCCTTCTCAA CTGGACGAAG TTGTATCCCC 
CTCCATGGTG TTAGTCCAGA AGTGAATGAA CTATTGATGG AAGCCCAGCT GCTCCAGGTA 
TCCCTTCCTG AAATTCAGGA ACTTTACCAG ACTTTACTTG CAAAGCCAAG CCCTGCTCAG 
CAGACTGACC GAAGCTCACC AGTGAGACCC AGCAGTGAGA AGAATGACTG TTGCCGAGGG 
AAGCGAGATG GAATTAACAG TCTTGAGAGA AAACTGAAGA GACGCCTGGA AAGAGAGGGC 
CTCTCCAGTG AGCGGTGGGA ACGAGTTAAG AAAATGCGGA CCCCCAAAAA GAAGAAAATC 
AAACTGAGCC ACCCCAAGGA CATGAACAAT TTCAAGTTAG AGAGAGAGCG TAGCTATGAA 
TTAGTTCGTT CTGCTGAAAC TCATTCCCTG CCCTCAGACA CATCCTATTC CGAACAGGAA 
GACTCTGAGG ATGAAGATGC CATCTGCCCA GCTGTGAGCT GCCTGCAGCC AGAAGGAGAT 
GAGGTGGACT GGGTCCAGTG TGATGGCAGC TGCAATCAGT GGTTTCATCA GGTCTGTGTT 
GGTGTCTCCC CAGAGATGGC AGAGAAAGAA GACTACATCT GTGTGCGCTG TACTGTGAAG 
GACGCACCAA GCCGAAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM5B ( NM_001347591.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001334520.1 Homo sapiens lysine demethylase 5B (KDM5B), transcript variant 3, mRNA.

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CloneID	OHu64357
Clone ID Related Accession (Same CDS sequence)	NM_001347591.1
Accession Version	NM_001347591.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4500bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-09
Organism	Homo sapiens(human)
Product	lysine-specific demethylase 5B isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC098934.3 and AC104463.3. On Dec 1, 2016 this sequence version replaced XM_011509088.2. Transcript Variant: This variant (3) lacks two exons in the 5' coding region compared to variant 1. It encodes isoform 3, which is shorter than isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.81627.1, SRR1660805.58809.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: upstream AUG and CDS extension is not conserved ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGAGGCGG CCACCACACT GCACCCAGGC CCGCGCCCGG CGCTGCCCCT CGGGGGCCCG 
GGCCCGCTGG GCGAGTTCCT GCCTCCACCC GAGTGCCCGG TCTTCGAACC CAGCTGGGAA 
GAGTTCGCGG ACCCCTTCGC TTTCATCCAC AAGATCCGGC CCATAGCCGA GCAGACTGGC 
ATCTGTAAGG TGCGGCCGCC GCCGGATTGG CAGCCACCAT TTGCATGTGA TGTTGATAAA 
CTTCATTTTA CGCCACGTAT CCAGAGACTG AATGAATTGG AGGCCCAAAC TCGTGTAAAA 
TTGAATTTCT TGGACCAGAT TGCAAAGTAC TGGGAGTTAC AGGGAAGTAC TCTGAAAATT 
CCACATGTGG AGAGGAAGAT CTTGGACTTA TTTCAGCTTA ATAAGTTAGT TGCAGAAGAA 
GGTGGATTTG CAGTTGTTTG CAAGGATAGA AAATGGACCA AAATTGCTAC CAAGATGGGG 
TTTGCTCCTG GCAAAGCAGT GGGCTCACAT ATCAGAGGGC ATTATGAACG AATTCTCAAC 
CCCTACAACT TATTCCTGTC CGGAGACAGC CTAAGGGCCA TGAATATTAA AATAGAACCC 
GAGGAGACAA CGGAAGCCAG AACTCATAAT CTGAGACGTC GAATGGGTTG TCCAACTCCA 
AAATGTGAAA ATGAGAAAGA AATGAAGAGT AGCATCAAGC AAGAACCTAT TGAGAGGAAA 
GATTATATTG TAGAAAATGA GAAGGAAAAG CCCAAGAGTC GATCTAAAAA AGCCACCAAT 
GCTGTGGACC TGTATGTCTG TCTTTTATGT GGCAGTGGCA ATGATGAAGA CCGGCTACTG 
TTGTGTGATG GCTGTGATGA CAGTTACCAT ACCTTTTGCT TGATCCCACC TCTCCATGAT 
GTTCCCAAGG GAGACTGGAG GTGTCCTAAG TGTTTGGCTC AGGAATGTAG TAAGCCACAA 
GAAGCATTTG GCTTTGAACA AGCAGCCAGG GACTATACCC TCCGTACTTT TGGGGAAATG 
GCAGATGCGT TCAAATCTGA TTACTTCAAC ATGCCAGTCC ATATGGTCCC CACAGAGCTT 
GTTGAGAAAG AATTTTGGAG ACTAGTAAGC ACTATTGAGG AGGATGTCAC AGTGGAATAT 
GGAGCTGACA TTGCCTCAAA GGAATTTGGC AGTGGCTTTC CTGTCCGAGA TGGGAAAATC 
AAACTCTCAC CTGAGGAAGA GGAGTATCTT GATAGTGGCT GGAATTTGAA CAACATGCCA 
GTGATGGAGC AGTCTGTCCT TGCACATATT ACTGCTGATA TATGTGGCAT GAAACTTCCT 
TGGTTGTATG TGGGAATGTG CTTTTCTTCA TTCTGTTGGC ACATTGAAGA CCACTGGAGC 
TATTCAATTA ACTACTTGCA CTGGGGTGAG CCAAAAACCT GGTATGGAGT CCCAGGGTAT 
GCTGCTGAGC AGCTAGAAAA TGTAATGAAG AAACTAGCTC CAGAACTCTT TGTGTCCCAG 
CCGGATCTCC TCCATCAGCT TGTGACCATC ATGAACCCCA ATACCCTGAT GACTCATGAA 
GTGCCTGTTT ACCGAACTAA TCAGTGTGCT GGGGAGTTTG TGATTACATT TCCAAGAGCC 
TACCACAGTG GTTTTAACCA GGGTTTTAAT TTTGCTGAGG CTGTTAACTT CTGCACTGTT 
GATTGGCTGC CATTAGGCCG ACAGTGTGTG GAGCATTATC GCTTGCTTCA TCGATATTGT 
GTGTTTTCCC ACGATGAGAT GATCTGCAAG ATGGCTTCCA AGGCTGATGT ATTAGATGTT 
GTAGTGGCTT CAACTGTTCA GAAAGACATG GCCATTATGA TTGAGGATGA GAAAGCTTTA 
AGAGAAACTG TCCGTAAATT GGGAGTGATT GATTCGGAAA GAATGGATTT TGAGCTGTTG 
CCAGATGATG AACGTCAGTG TGTAAAATGC AAAACTACAT GCTTCATGTC TGCCATCTCC 
TGTTCTTGTA AACCTGGCCT TCTTGTTTGC CTGCATCATG TAAAAGAATT GTGTTCCTGT 
CCTCCTTACA AATATAAATT GCGGTATAGG TACACGCTGG ATGATCTCTA CCCTATGATG 
AATGCATTGA AGCTTCGAGC AGAATCTTAC AACGAATGGG CCTTGAATGT GAATGAAGCT 
TTGGAGGCAA AGATCAACAA GAAGAAAAGC CTTGTCAGCT TCAAGGCTTT AATTGAAGAA 
TCTGAAATGA AGAAATTCCC AGACAATGAT CTTTTGCGAC ACCTTCGCCT AGTCACACAG 
GATGCAGAGA AGTGTGCCTC TGTTGCGCAG CAGTTGCTTA ATGGCAAAAG GCAAACTAGA 
TATCGATCTG GTGGAGGGAA ATCCCAAAAT CAGTTGACAG TGAATGAGCT CCGGCAGTTT 
GTAACACAGC TGTATGCTCT TCCATGTGTC CTCAGTCAGA CACCATTACT AAAGGATCTC 
TTGAATCGTG TAGAAGATTT TCAACAGCAT AGTCAGAAAC TACTCTCTGA GGAAACGCCT 
AGTGCTGCGG AGCTGCAGGA CTTGCTAGAT GTCAGCTTTG AATTTGATGT TGAACTTCCA 
CAGCTTGCTG AGATGCGTAT CCGTTTGGAA CAAGCCCGTT GGCTAGAAGA GGTGCAGCAA 
GCTTGCCTAG ACCCCAGCTC CCTTACTTTA GATGATATGA GACGTCTCAT AGACCTAGGG 
GTAGGGCTGG CCCCGTATTC AGCAGTGGAG AAAGCTATGG CCCGGCTGCA GGAACTGCTC 
ACAGTGTCAG AGCACTGGGA CGACAAAGCC AAGAGTCTCC TCAAGGCCAG GCCACGACAT 
TCATTGAATA GCCTTGCTAC GGCAGTAAAG GAAATCGAAG AGATCCCTGC ATATCTGCCC 
AATGGTGCGG CTCTGAAAGA CTCAGTGCAG AGAGCCAGAG ACTGGCTTCA GGATGTAGAG 
GGCCTGCAGG CTGGAGGACG TGTGCCAGTG TTAGACACAC TCATAGAACT TGTTACACGA 
GGCCGATCTA TCCCCGTACA TCTGAATTCT TTGCCAAGAC TGGAAACCCT AGTAGCTGAG 
GTTCAGGCTT GGAAAGAATG TGCTGTTAAT ACATTCTTGA CTGAGAATTC TCCATATTCT 
CTCTTAGAGG TGCTGTGTCC TCGATGTGAT ATTGGCCTTT TGGGATTGAA AAGGAAGCAG 
AGAAAGTTAA AGGAGCCCTT GCCAAATGGA AAGAAAAAAA GCACCAAATT AGAGAGTCTG 
AGTGACCTGG AGAGAGCTTT AACTGAAAGC AAGGAGACTG CTTCAGCTAT GGCAACTCTT 
GGGGAAGCTC GCCTAAGGGA AATGGAAGCC TTGCAGTCTC TCAGACTCGC CAATGAAGGG 
AAATTGCTGT CGCCTCTCCA AGATGTGGAT ATAAAAATCT GCCTATGTCA GAAGGCCCCA 
GCTGCCCCTA TGATTCAATG TGAACTCTGC AGGGATGCTT TCCACACCAG TTGTGTGGCG 
GTACCCAGTA TTTCACAGGG CCTGCGAATC TGGCTTTGTC CCCATTGTCG GAGGTCAGAG 
AAACCTCCAT TAGAGAAAAT TCTGCCCCTG CTCGCCTCCC TTCAGCGTAT CCGAGTTCGC 
CTTCCTGAGG GAGATGCACT TCGATATATG ATTGAAAGAA CCGTGAACTG GCAGCACAGA 
GCCCAGCAAC TGCTTTCGTC AGGGAATCTT AAATTTGTGC AAGATCGAGT GGGCTCAGGA 
CTGTTATATA GCAGATGGCA AGCCTCAGCA GGACAGGTGT CAGACACAAA CAAGGTATCT 
CAACCTCCTG GCACAACATC ATTTTCTTTG CCTGATGACT GGGACAACAG AACCTCATAT 
TTGCACTCCC CCTTCTCAAC TGGACGAAGT TGTATCCCCC TCCATGGTGT TAGTCCAGAA 
GTGAATGAAC TATTGATGGA AGCCCAGCTG CTCCAGGTAT CCCTTCCTGA AATTCAGGAA 
CTTTACCAGA CTTTACTTGC AAAGCCAAGC CCTGCTCAGC AGACTGACCG AAGCTCACCA 
GTGAGACCCA GCAGTGAGAA GAATGACTGT TGCCGAGGGA AGCGAGATGG AATTAACAGT 
CTTGAGAGAA AACTGAAGAG ACGCCTGGAA AGAGAGGGCC TCTCCAGTGA GCGGTGGGAA 
CGAGTTAAGA AAATGCGGAC CCCCAAAAAG AAGAAAATCA AACTGAGCCA CCCCAAGGAC 
ATGAACAATT TCAAGTTAGA GAGAGAGCGT AGCTATGAAT TAGTTCGTTC TGCTGAAACT 
CATTCCCTGC CCTCAGACAC ATCCTATTCC GAACAGGAAG ACTCTGAGGA TGAAGATGCC 
ATCTGCCCAG CTGTGAGCTG CCTGCAGCCA GAAGGAGATG AGGTGGACTG GGTCCAGTGT 
GATGGCAGCT GCAATCAGTG GTTTCATCAG GTCTGTGTTG GTGTCTCCCC AGAGATGGCA 
GAGAAAGAAG ACTACATCTG TGTGCGCTGT ACTGTGAAGG ACGCACCAAG CCGAAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001334520.1
CDS	1166..5665
Translation	MEAATTLHPGPRPALPLGGPGPLGEFLPPPECPVFEPSWEEFADPFAFIHKIRPIAEQTGICKVRPPPDWQPPFACDVDKLHFTPRIQRLNELEAQTRVKLNFLDQIAKYWELQGSTLKIPHVERKILDLFQLNKLVAEEGGFAVVCKDRKWTKIATKMGFAPGKAVGSHIRGHYERILNPYNLFLSGDSLRAMNIKIEPEETTEARTHNLRRRMGCPTPKCENEKEMKSSIKQEPIERKDYIVENEKEKPKSRSKKATNAVDLYVCLLCGSGNDEDRLLLCDGCDDSYHTFCLIPPLHDVPKGDWRCPKCLAQECSKPQEAFGFEQAARDYTLRTFGEMADAFKSDYFNMPVHMVPTELVEKEFWRLVSTIEEDVTVEYGADIASKEFGSGFPVRDGKIKLSPEEEEYLDSGWNLNNMPVMEQSVLAHITADICGMKLPWLYVGMCFSSFCWHIEDHWSYSINYLHWGEPKTWYGVPGYAAEQLENVMKKLAPELFVSQPDLLHQLVTIMNPNTLMTHEVPVYRTNQCAGEFVITFPRAYHSGFNQGFNFAEAVNFCTVDWLPLGRQCVEHYRLLHRYCVFSHDEMICKMASKADVLDVVVASTVQKDMAIMIEDEKALRETVRKLGVIDSERMDFELLPDDERQCVKCKTTCFMSAISCSCKPGLLVCLHHVKELCSCPPYKYKLRYRYTLDDLYPMMNALKLRAESYNEWALNVNEALEAKINKKKSLVSFKALIEESEMKKFPDNDLLRHLRLVTQDAEKCASVAQQLLNGKRQTRYRSGGGKSQNQLTVNELRQFVTQLYALPCVLSQTPLLKDLLNRVEDFQQHSQKLLSEETPSAAELQDLLDVSFEFDVELPQLAEMRIRLEQARWLEEVQQACLDPSSLTLDDMRRLIDLGVGLAPYSAVEKAMARLQELLTVSEHWDDKAKSLLKARPRHSLNSLATAVKEIEEIPAYLPNGAALKDSVQRARDWLQDVEGLQAGGRVPVLDTLIELVTRGRSIPVHLNSLPRLETLVAEVQAWKECAVNTFLTENSPYSLLEVLCPRCDIGLLGLKRKQRKLKEPLPNGKKKSTKLESLSDLERALTESKETASAMATLGEARLREMEALQSLRLANEGKLLSPLQDVDIKICLCQKAPAAPMIQCELCRDAFHTSCVAVPSISQGLRIWLCPHCRRSEKPPLEKILPLLASLQRIRVRLPEGDALRYMIERTVNWQHRAQQLLSSGNLKFVQDRVGSGLLYSRWQASAGQVSDTNKVSQPPGTTSFSLPDDWDNRTSYLHSPFSTGRSCIPLHGVSPEVNELLMEAQLLQVSLPEIQELYQTLLAKPSPAQQTDRSSPVRPSSEKNDCCRGKRDGINSLERKLKRRLEREGLSSERWERVKKMRTPKKKKIKLSHPKDMNNFKLERERSYELVRSAETHSLPSDTSYSEQEDSEDEDAICPAVSCLQPEGDEVDWVQCDGSCNQWFHQVCVGVSPEMAEKEDYICVRCTVKDAPSRK

Target ORF information:

RefSeq Version	NM_001347591.1
Organism	Homo sapiens(human)
Definition	Homo sapiens lysine demethylase 5B (KDM5B), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001347591.1

ORF Insert Sequence:

ATGGAGGCGG CCACCACACT GCACCCAGGC CCGCGCCCGG CGCTGCCCCT CGGGGGCCCG 
GGCCCGCTGG GCGAGTTCCT GCCTCCACCC GAGTGCCCGG TCTTCGAACC CAGCTGGGAA 
GAGTTCGCGG ACCCCTTCGC TTTCATCCAC AAGATCCGGC CCATAGCCGA GCAGACTGGC 
ATCTGTAAGG TGCGGCCGCC GCCGGATTGG CAGCCACCAT TTGCATGTGA TGTTGATAAA 
CTTCATTTTA CGCCACGTAT CCAGAGACTG AATGAATTGG AGGCCCAAAC TCGTGTAAAA 
TTGAATTTCT TGGACCAGAT TGCAAAGTAC TGGGAGTTAC AGGGAAGTAC TCTGAAAATT 
CCACATGTGG AGAGGAAGAT CTTGGACTTA TTTCAGCTTA ATAAGTTAGT TGCAGAAGAA 
GGTGGATTTG CAGTTGTTTG CAAGGATAGA AAATGGACCA AAATTGCTAC CAAGATGGGG 
TTTGCTCCTG GCAAAGCAGT GGGCTCACAT ATCAGAGGGC ATTATGAACG AATTCTCAAC 
CCCTACAACT TATTCCTGTC CGGAGACAGC CTAAGGGCCA TGAATATTAA AATAGAACCC 
GAGGAGACAA CGGAAGCCAG AACTCATAAT CTGAGACGTC GAATGGGTTG TCCAACTCCA 
AAATGTGAAA ATGAGAAAGA AATGAAGAGT AGCATCAAGC AAGAACCTAT TGAGAGGAAA 
GATTATATTG TAGAAAATGA GAAGGAAAAG CCCAAGAGTC GATCTAAAAA AGCCACCAAT 
GCTGTGGACC TGTATGTCTG TCTTTTATGT GGCAGTGGCA ATGATGAAGA CCGGCTACTG 
TTGTGTGATG GCTGTGATGA CAGTTACCAT ACCTTTTGCT TGATCCCACC TCTCCATGAT 
GTTCCCAAGG GAGACTGGAG GTGTCCTAAG TGTTTGGCTC AGGAATGTAG TAAGCCACAA 
GAAGCATTTG GCTTTGAACA AGCAGCCAGG GACTATACCC TCCGTACTTT TGGGGAAATG 
GCAGATGCGT TCAAATCTGA TTACTTCAAC ATGCCAGTCC ATATGGTCCC CACAGAGCTT 
GTTGAGAAAG AATTTTGGAG ACTAGTAAGC ACTATTGAGG AGGATGTCAC AGTGGAATAT 
GGAGCTGACA TTGCCTCAAA GGAATTTGGC AGTGGCTTTC CTGTCCGAGA TGGGAAAATC 
AAACTCTCAC CTGAGGAAGA GGAGTATCTT GATAGTGGCT GGAATTTGAA CAACATGCCA 
GTGATGGAGC AGTCTGTCCT TGCACATATT ACTGCTGATA TATGTGGCAT GAAACTTCCT 
TGGTTGTATG TGGGAATGTG CTTTTCTTCA TTCTGTTGGC ACATTGAAGA CCACTGGAGC 
TATTCAATTA ACTACTTGCA CTGGGGTGAG CCAAAAACCT GGTATGGAGT CCCAGGGTAT 
GCTGCTGAGC AGCTAGAAAA TGTAATGAAG AAACTAGCTC CAGAACTCTT TGTGTCCCAG 
CCGGATCTCC TCCATCAGCT TGTGACCATC ATGAACCCCA ATACCCTGAT GACTCATGAA 
GTGCCTGTTT ACCGAACTAA TCAGTGTGCT GGGGAGTTTG TGATTACATT TCCAAGAGCC 
TACCACAGTG GTTTTAACCA GGGTTTTAAT TTTGCTGAGG CTGTTAACTT CTGCACTGTT 
GATTGGCTGC CATTAGGCCG ACAGTGTGTG GAGCATTATC GCTTGCTTCA TCGATATTGT 
GTGTTTTCCC ACGATGAGAT GATCTGCAAG ATGGCTTCCA AGGCTGATGT ATTAGATGTT 
GTAGTGGCTT CAACTGTTCA GAAAGACATG GCCATTATGA TTGAGGATGA GAAAGCTTTA 
AGAGAAACTG TCCGTAAATT GGGAGTGATT GATTCGGAAA GAATGGATTT TGAGCTGTTG 
CCAGATGATG AACGTCAGTG TGTAAAATGC AAAACTACAT GCTTCATGTC TGCCATCTCC 
TGTTCTTGTA AACCTGGCCT TCTTGTTTGC CTGCATCATG TAAAAGAATT GTGTTCCTGT 
CCTCCTTACA AATATAAATT GCGGTATAGG TACACGCTGG ATGATCTCTA CCCTATGATG 
AATGCATTGA AGCTTCGAGC AGAATCTTAC AACGAATGGG CCTTGAATGT GAATGAAGCT 
TTGGAGGCAA AGATCAACAA GAAGAAAAGC CTTGTCAGCT TCAAGGCTTT AATTGAAGAA 
TCTGAAATGA AGAAATTCCC AGACAATGAT CTTTTGCGAC ACCTTCGCCT AGTCACACAG 
GATGCAGAGA AGTGTGCCTC TGTTGCGCAG CAGTTGCTTA ATGGCAAAAG GCAAACTAGA 
TATCGATCTG GTGGAGGGAA ATCCCAAAAT CAGTTGACAG TGAATGAGCT CCGGCAGTTT 
GTAACACAGC TGTATGCTCT TCCATGTGTC CTCAGTCAGA CACCATTACT AAAGGATCTC 
TTGAATCGTG TAGAAGATTT TCAACAGCAT AGTCAGAAAC TACTCTCTGA GGAAACGCCT 
AGTGCTGCGG AGCTGCAGGA CTTGCTAGAT GTCAGCTTTG AATTTGATGT TGAACTTCCA 
CAGCTTGCTG AGATGCGTAT CCGTTTGGAA CAAGCCCGTT GGCTAGAAGA GGTGCAGCAA 
GCTTGCCTAG ACCCCAGCTC CCTTACTTTA GATGATATGA GACGTCTCAT AGACCTAGGG 
GTAGGGCTGG CCCCGTATTC AGCAGTGGAG AAAGCTATGG CCCGGCTGCA GGAACTGCTC 
ACAGTGTCAG AGCACTGGGA CGACAAAGCC AAGAGTCTCC TCAAGGCCAG GCCACGACAT 
TCATTGAATA GCCTTGCTAC GGCAGTAAAG GAAATCGAAG AGATCCCTGC ATATCTGCCC 
AATGGTGCGG CTCTGAAAGA CTCAGTGCAG AGAGCCAGAG ACTGGCTTCA GGATGTAGAG 
GGCCTGCAGG CTGGAGGACG TGTGCCAGTG TTAGACACAC TCATAGAACT TGTTACACGA 
GGCCGATCTA TCCCCGTACA TCTGAATTCT TTGCCAAGAC TGGAAACCCT AGTAGCTGAG 
GTTCAGGCTT GGAAAGAATG TGCTGTTAAT ACATTCTTGA CTGAGAATTC TCCATATTCT 
CTCTTAGAGG TGCTGTGTCC TCGATGTGAT ATTGGCCTTT TGGGATTGAA AAGGAAGCAG 
AGAAAGTTAA AGGAGCCCTT GCCAAATGGA AAGAAAAAAA GCACCAAATT AGAGAGTCTG 
AGTGACCTGG AGAGAGCTTT AACTGAAAGC AAGGAGACTG CTTCAGCTAT GGCAACTCTT 
GGGGAAGCTC GCCTAAGGGA AATGGAAGCC TTGCAGTCTC TCAGACTCGC CAATGAAGGG 
AAATTGCTGT CGCCTCTCCA AGATGTGGAT ATAAAAATCT GCCTATGTCA GAAGGCCCCA 
GCTGCCCCTA TGATTCAATG TGAACTCTGC AGGGATGCTT TCCACACCAG TTGTGTGGCG 
GTACCCAGTA TTTCACAGGG CCTGCGAATC TGGCTTTGTC CCCATTGTCG GAGGTCAGAG 
AAACCTCCAT TAGAGAAAAT TCTGCCCCTG CTCGCCTCCC TTCAGCGTAT CCGAGTTCGC 
CTTCCTGAGG GAGATGCACT TCGATATATG ATTGAAAGAA CCGTGAACTG GCAGCACAGA 
GCCCAGCAAC TGCTTTCGTC AGGGAATCTT AAATTTGTGC AAGATCGAGT GGGCTCAGGA 
CTGTTATATA GCAGATGGCA AGCCTCAGCA GGACAGGTGT CAGACACAAA CAAGGTATCT 
CAACCTCCTG GCACAACATC ATTTTCTTTG CCTGATGACT GGGACAACAG AACCTCATAT 
TTGCACTCCC CCTTCTCAAC TGGACGAAGT TGTATCCCCC TCCATGGTGT TAGTCCAGAA 
GTGAATGAAC TATTGATGGA AGCCCAGCTG CTCCAGGTAT CCCTTCCTGA AATTCAGGAA 
CTTTACCAGA CTTTACTTGC AAAGCCAAGC CCTGCTCAGC AGACTGACCG AAGCTCACCA 
GTGAGACCCA GCAGTGAGAA GAATGACTGT TGCCGAGGGA AGCGAGATGG AATTAACAGT 
CTTGAGAGAA AACTGAAGAG ACGCCTGGAA AGAGAGGGCC TCTCCAGTGA GCGGTGGGAA 
CGAGTTAAGA AAATGCGGAC CCCCAAAAAG AAGAAAATCA AACTGAGCCA CCCCAAGGAC 
ATGAACAATT TCAAGTTAGA GAGAGAGCGT AGCTATGAAT TAGTTCGTTC TGCTGAAACT 
CATTCCCTGC CCTCAGACAC ATCCTATTCC GAACAGGAAG ACTCTGAGGA TGAAGATGCC 
ATCTGCCCAG CTGTGAGCTG CCTGCAGCCA GAAGGAGATG AGGTGGACTG GGTCCAGTGT 
GATGGCAGCT GCAATCAGTG GTTTCATCAG GTCTGTGTTG GTGTCTCCCC AGAGATGGCA 
GAGAAAGAAG ACTACATCTG TGTGCGCTGT ACTGTGAAGG ACGCACCAAG CCGAAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	KDM5B
Entrez Gene ID	10765
Full Name	lysine demethylase 5B
Synonyms	CT31,JARID1B,PLU-1,PLU1,PPP1R98,PUT1,RBBP2H1A,RBP2-H1
General protein information	Preferred Names lysine demethylase 5B Names lysine-specific demethylase 5B cancer/testis antigen 31 histone demethylase JARID1B jumonji, AT rich interactive domain 1B jumonji/ARID domain-containing protein 1B lysine (K)-specific demethylase 5B protein phosphatase 1, regulatory subunit 98 putative DNA/chromatin binding motif retinoblastoma-binding protein 2 homolog 1 retinoblastoma-binding protein 2, homolog 1A
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1q32.1
Summary	This gene encodes a lysine-specific histone demethylase that belongs to the jumonji/ARID domain-containing family of histone demethylases. The encoded protein is capable of demethylating tri-, di- and monomethylated lysine 4 of histone H3. This protein plays a role in the transcriptional repression or certain tumor suppressor genes and is upregulated in certain cancer cells. This protein may also play a role in genome stability and DNA repair. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Nov 2016].
Disorder MIM:	605393

Bos taurus (cattle)	KDM5B	XP_002694248.2
Rattus norvegicus (Norway rat)	Kdm5b	NP_001100647.1
Canis lupus familiaris (dog)	KDM5B	XP_005622266.1
Gallus gallus (chicken)	KDM5B	NP_001026200.1
Danio rerio (zebrafish)	kdm5bb	NP_001002166.1
Xenopus tropicalis (tropical clawed frog)	kdm5b	XP_004910700.1
Mus musculus (house mouse)	Kdm5b	NP_690855.2
Caenorhabditis elegans (roundworm)	rbr-2	NP_502032.2
Pan troglodytes (chimpanzee)	KDM5B	XP_514104.3
Homo sapiens (human)	KDM5B	NP_006609.3
	LOC706437	XP_002808303.1

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KDM5B cDNA ORF clone, Homo sapiens(human)

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