LEFTY1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	LEFTY1
Entrez Gene ID	10637
Full Name	left-right determination factor 1
Synonyms	LEFTB,LEFTYB
General protein information	Preferred Names left-right determination factor 1 Names left-right determination factor 1 left-right determination factor B protein lefty-1 protein lefty-B
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1q42.12
Summary	This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate the mature protein, which plays a role in left-right asymmetry determination of organ systems during development. This gene is closely linked to both a related family member and a related pseudogene. [provided by RefSeq, Aug 2016].
Disorder MIM:	603037

mRNA and Protein(s)

mRNA	Protein	Name
NM_020997.3	NP_066277.1	left-right determination factor 1 preproprotein
NM_020997.4	NP_066277.1	left-right determination factor 1 preproprotein

Pathways from BioSystems

Homologies

Homo sapiens (human)	LEFTY1	NP_066277.1
	LEFTY1	XP_001092988.1
Mus musculus (house mouse)	Lefty1	NP_034224.1
Rattus norvegicus (Norway rat)	Lefty1	NP_001102550.1
Pan troglodytes (chimpanzee)	LEFTY1	XP_001138066.1

Gene Ontology
Bibliography

Related articles in PubMed

A functional role of LEFTY during progesterone therapy for endometrial carcinoma.
Fei W, Kijima D, Hashimoto M, Hashimura M, Oguri Y, Kajita S, Matsumoto T, Yokoi A, Saegusa M
Cell communication and signaling : CCS15(1)56(2017 Dec)

Lefty inhibits in vitro decidualization by regulating P57 and cyclin D1 expressions.
Li H, Li H, Bai L, Yu H
Cell biochemistry and function32(8)657-64(2014 Dec)

Wnt3a-dependent and -independent protein interaction networks of chromatin-bound ?-catenin in mouse embryonic stem cells.
Yakulov T, Raggioli A, Franz H, Kemler R
Molecular & cellular proteomics : MCP12(7)1980-94(2013 Jul)

Suppression of Lefty expression in induced pluripotent cancer cells.
Saito A, Ochiai H, Okada S, Miyata N, Azuma T
FASEB journal : official publication of the Federation of American Societies for Experimental Biology27(6)2165-74(2013 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Lefty1 could regulates the cell cycle via modulating the expressions of P57 and cyclin D1 and then inhibit the decidualization in vitro.
Title: Lefty inhibits in vitro decidualization by regulating P57 and cyclin D1 expressions.

The mRNA levels of LSD1 and beta-catenin are inversely correlated with the levels of Lefty1 in pancreas and breast tumors.
Title: Wnt3a-dependent and -independent protein interaction networks of chromatin-bound ?-catenin in mouse embryonic stem cells.

Reprogrammed cancer cells share the mechanism for expression of Lefty with induced pluripotent cancer cells (iPS), thus contributing to the cancerous transformation of iPS cells.
Title: Suppression of Lefty expression in induced pluripotent cancer cells.

Data suggest that Lefty is a novel TGF-beta target molecule that mediates growth inhibition of pancreatic cancer cells.
Title: Transforming growth factor ? and Ras/MEK/ERK signaling regulate the expression level of a novel tumor suppressor Lefty.

Findings show that during embryonic development, EBAF/LEFTY B plays important roles in decidualization and embryo implantation.
Title: Isolation, characterization, and function of EBAF/LEFTY B: role in infertility.

The following LEFTY1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LEFTY1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu03639	NM_020997.4 Latest version!	Homo sapiens left-right determination factor 1 (LEFTY1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
View Old Accession Versions >>
OHu03639	NM_020997.3	Homo sapiens left-right determination factor 1 (LEFTY1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LEFTY1 ( NM_020997.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_066277.1 Homo sapiens left-right determination factor 1 (LEFTY1), mRNA.

Price & Availability↑

CloneID	OHu03639
Clone ID Related Accession (Same CDS sequence)	NM_020997.3 , NM_020997.4
Accession Version	NM_020997.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1101bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	left-right determination factor 1 preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK222714.1 and AF081512.1. On Mar 10, 2011 this sequence version replaced NM_020997.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK222714.1, AY358873.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1968189, SAMEA1968540 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCAGCCCC TGTGGCTCTG CTGGGCACTC TGGGTGTTGC CCCTGGCCAG CCCCGGGGCC 
GCCCTGACCG GGGAGCAGCT CCTGGGCAGC CTGCTGCGGC AGCTGCAGCT CAAAGAGGTG 
CCCACCCTGG ACAGGGCCGA CATGGAGGAG CTGGTCATCC CCACCCACGT GAGGGCCCAG 
TACGTGGCCC TGCTGCAGCG CAGCCACGGG GACCGCTCCC GCGGAAAGAG GTTCAGCCAG 
AGCTTCCGAG AGGTGGCCGG CAGGTTCCTG GCGTTGGAGG CCAGCACACA CCTGCTGGTG 
TTCGGCATGG AGCAGCGGCT GCCGCCCAAC AGCGAGCTGG TGCAGGCCGT GCTGCGGCTC 
TTCCAGGAGC CGGTCCCCAA GGCCGCGCTG CACAGGCACG GGCGGCTGTC CCCGCGCAGC 
GCCCGGGCCC GGGTGACCGT CGAGTGGCTG CGCGTCCGCG ACGACGGCTC CAACCGCACC 
TCCCTCATCG ACTCCAGGCT GGTGTCCGTC CACGAGAGCG GCTGGAAGGC CTTCGACGTG 
ACCGAGGCCG TGAACTTCTG GCAGCAGCTG AGCCGGCCCC GGCAGCCGCT GCTGCTACAG 
GTGTCGGTGC AGAGGGAGCA TCTGGGCCCG CTGGCGTCCG GCGCCCACAA GCTGGTCCGC 
TTTGCCTCGC AGGGGGCGCC AGCCGGGCTT GGGGAGCCCC AGCTGGAGCT GCACACCCTG 
GACCTTGGGG ACTATGGAGC TCAGGGCGAC TGTGACCCTG AAGCACCAAT GACCGAGGGC 
ACCCGCTGCT GCCGCCAGGA GATGTACATT GACCTGCAGG GGATGAAGTG GGCCGAGAAC 
TGGGTGCTGG AGCCCCCGGG CTTCCTGGCT TATGAGTGTG TGGGCACCTG CCGGCAGCCC 
CCGGAGGCCC TGGCCTTCAA GTGGCCGTTT CTGGGGCCTC GACAGTGCAT CGCCTCGGAG 
ACTGACTCGC TGCCCATGAT CGTCAGCATC AAGGAGGGAG GCAGGACCAG GCCCCAGGTG 
GTCAGCCTGC CCAACATGAG GGTGCAGAAG TGCAGCTGTG CCTCGGATGG TGCGCTCGTG 
CCAAGGAGGC TCCAGCCATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_066277.1
CDS	81..1181
Translation	MQPLWLCWALWVLPLASPGAALTGEQLLGSLLRQLQLKEVPTLDRADMEELVIPTHVRAQYVALLQRSHGDRSRGKRFSQSFREVAGRFLALEASTHLLVFGMEQRLPPNSELVQAVLRLFQEPVPKAALHRHGRLSPRSARARVTVEWLRVRDDGSNRTSLIDSRLVSVHESGWKAFDVTEAVNFWQQLSRPRQPLLLQVSVQREHLGPLASGAHKLVRFASQGAPAGLGEPQLELHTLDLGDYGAQGDCDPEAPMTEGTRCCRQEMYIDLQGMKWAENWVLEPPGFLAYECVGTCRQPPEALAFKWPFLGPRQCIASETDSLPMIVSIKEGGRTRPQVVSLPNMRVQKCSCASDGALVPRRLQP

Target ORF information:

RefSeq Version	NM_020997.3
Organism	Homo sapiens(human)
Definition	Homo sapiens left-right determination factor 1 (LEFTY1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_020997.3

ORF Insert Sequence:

ATGCAGCCCC TGTGGCTCTG CTGGGCACTC TGGGTGTTGC CCCTGGCCAG CCCCGGGGCC 
GCCCTGACCG GGGAGCAGCT CCTGGGCAGC CTGCTGCGGC AGCTGCAGCT CAAAGAGGTG 
CCCACCCTGG ACAGGGCCGA CATGGAGGAG CTGGTCATCC CCACCCACGT GAGGGCCCAG 
TACGTGGCCC TGCTGCAGCG CAGCCACGGG GACCGCTCCC GCGGAAAGAG GTTCAGCCAG 
AGCTTCCGAG AGGTGGCCGG CAGGTTCCTG GCGTTGGAGG CCAGCACACA CCTGCTGGTG 
TTCGGCATGG AGCAGCGGCT GCCGCCCAAC AGCGAGCTGG TGCAGGCCGT GCTGCGGCTC 
TTCCAGGAGC CGGTCCCCAA GGCCGCGCTG CACAGGCACG GGCGGCTGTC CCCGCGCAGC 
GCCCGGGCCC GGGTGACCGT CGAGTGGCTG CGCGTCCGCG ACGACGGCTC CAACCGCACC 
TCCCTCATCG ACTCCAGGCT GGTGTCCGTC CACGAGAGCG GCTGGAAGGC CTTCGACGTG 
ACCGAGGCCG TGAACTTCTG GCAGCAGCTG AGCCGGCCCC GGCAGCCGCT GCTGCTACAG 
GTGTCGGTGC AGAGGGAGCA TCTGGGCCCG CTGGCGTCCG GCGCCCACAA GCTGGTCCGC 
TTTGCCTCGC AGGGGGCGCC AGCCGGGCTT GGGGAGCCCC AGCTGGAGCT GCACACCCTG 
GACCTTGGGG ACTATGGAGC TCAGGGCGAC TGTGACCCTG AAGCACCAAT GACCGAGGGC 
ACCCGCTGCT GCCGCCAGGA GATGTACATT GACCTGCAGG GGATGAAGTG GGCCGAGAAC 
TGGGTGCTGG AGCCCCCGGG CTTCCTGGCT TATGAGTGTG TGGGCACCTG CCGGCAGCCC 
CCGGAGGCCC TGGCCTTCAA GTGGCCGTTT CTGGGGCCTC GACAGTGCAT CGCCTCGGAG 
ACTGACTCGC TGCCCATGAT CGTCAGCATC AAGGAGGGAG GCAGGACCAG GCCCCAGGTG 
GTCAGCCTGC CCAACATGAG GGTGCAGAAG TGCAGCTGTG CCTCGGATGG TGCGCTCGTG 
CCAAGGAGGC TCCAGCCATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LEFTY1 ( NM_020997.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_066277.1 Homo sapiens left-right determination factor 1 (LEFTY1), mRNA.

Price & Availability↑

CloneID	OHu03639
Clone ID Related Accession (Same CDS sequence)	NM_020997.3 , NM_020997.4
Accession Version	NM_020997.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1101bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	left-right determination factor 1 preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK222714.1 and AF081512.1. On Nov 23, 2018 this sequence version replaced NM_020997.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK222714.1, AY358873.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1968189, SAMEA1968540 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000272134.5/ ENSP00000272134.5 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCAGCCCC TGTGGCTCTG CTGGGCACTC TGGGTGTTGC CCCTGGCCAG CCCCGGGGCC 
GCCCTGACCG GGGAGCAGCT CCTGGGCAGC CTGCTGCGGC AGCTGCAGCT CAAAGAGGTG 
CCCACCCTGG ACAGGGCCGA CATGGAGGAG CTGGTCATCC CCACCCACGT GAGGGCCCAG 
TACGTGGCCC TGCTGCAGCG CAGCCACGGG GACCGCTCCC GCGGAAAGAG GTTCAGCCAG 
AGCTTCCGAG AGGTGGCCGG CAGGTTCCTG GCGTTGGAGG CCAGCACACA CCTGCTGGTG 
TTCGGCATGG AGCAGCGGCT GCCGCCCAAC AGCGAGCTGG TGCAGGCCGT GCTGCGGCTC 
TTCCAGGAGC CGGTCCCCAA GGCCGCGCTG CACAGGCACG GGCGGCTGTC CCCGCGCAGC 
GCCCGGGCCC GGGTGACCGT CGAGTGGCTG CGCGTCCGCG ACGACGGCTC CAACCGCACC 
TCCCTCATCG ACTCCAGGCT GGTGTCCGTC CACGAGAGCG GCTGGAAGGC CTTCGACGTG 
ACCGAGGCCG TGAACTTCTG GCAGCAGCTG AGCCGGCCCC GGCAGCCGCT GCTGCTACAG 
GTGTCGGTGC AGAGGGAGCA TCTGGGCCCG CTGGCGTCCG GCGCCCACAA GCTGGTCCGC 
TTTGCCTCGC AGGGGGCGCC AGCCGGGCTT GGGGAGCCCC AGCTGGAGCT GCACACCCTG 
GACCTTGGGG ACTATGGAGC TCAGGGCGAC TGTGACCCTG AAGCACCAAT GACCGAGGGC 
ACCCGCTGCT GCCGCCAGGA GATGTACATT GACCTGCAGG GGATGAAGTG GGCCGAGAAC 
TGGGTGCTGG AGCCCCCGGG CTTCCTGGCT TATGAGTGTG TGGGCACCTG CCGGCAGCCC 
CCGGAGGCCC TGGCCTTCAA GTGGCCGTTT CTGGGGCCTC GACAGTGCAT CGCCTCGGAG 
ACTGACTCGC TGCCCATGAT CGTCAGCATC AAGGAGGGAG GCAGGACCAG GCCCCAGGTG 
GTCAGCCTGC CCAACATGAG GGTGCAGAAG TGCAGCTGTG CCTCGGATGG TGCGCTCGTG 
CCAAGGAGGC TCCAGCCATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_066277.1
CDS	81..1181
Translation	MQPLWLCWALWVLPLASPGAALTGEQLLGSLLRQLQLKEVPTLDRADMEELVIPTHVRAQYVALLQRSHGDRSRGKRFSQSFREVAGRFLALEASTHLLVFGMEQRLPPNSELVQAVLRLFQEPVPKAALHRHGRLSPRSARARVTVEWLRVRDDGSNRTSLIDSRLVSVHESGWKAFDVTEAVNFWQQLSRPRQPLLLQVSVQREHLGPLASGAHKLVRFASQGAPAGLGEPQLELHTLDLGDYGAQGDCDPEAPMTEGTRCCRQEMYIDLQGMKWAENWVLEPPGFLAYECVGTCRQPPEALAFKWPFLGPRQCIASETDSLPMIVSIKEGGRTRPQVVSLPNMRVQKCSCASDGALVPRRLQP

Target ORF information:

RefSeq Version	NM_020997.4
Organism	Homo sapiens(human)
Definition	Homo sapiens left-right determination factor 1 (LEFTY1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_020997.4

ORF Insert Sequence:

ATGCAGCCCC TGTGGCTCTG CTGGGCACTC TGGGTGTTGC CCCTGGCCAG CCCCGGGGCC 
GCCCTGACCG GGGAGCAGCT CCTGGGCAGC CTGCTGCGGC AGCTGCAGCT CAAAGAGGTG 
CCCACCCTGG ACAGGGCCGA CATGGAGGAG CTGGTCATCC CCACCCACGT GAGGGCCCAG 
TACGTGGCCC TGCTGCAGCG CAGCCACGGG GACCGCTCCC GCGGAAAGAG GTTCAGCCAG 
AGCTTCCGAG AGGTGGCCGG CAGGTTCCTG GCGTTGGAGG CCAGCACACA CCTGCTGGTG 
TTCGGCATGG AGCAGCGGCT GCCGCCCAAC AGCGAGCTGG TGCAGGCCGT GCTGCGGCTC 
TTCCAGGAGC CGGTCCCCAA GGCCGCGCTG CACAGGCACG GGCGGCTGTC CCCGCGCAGC 
GCCCGGGCCC GGGTGACCGT CGAGTGGCTG CGCGTCCGCG ACGACGGCTC CAACCGCACC 
TCCCTCATCG ACTCCAGGCT GGTGTCCGTC CACGAGAGCG GCTGGAAGGC CTTCGACGTG 
ACCGAGGCCG TGAACTTCTG GCAGCAGCTG AGCCGGCCCC GGCAGCCGCT GCTGCTACAG 
GTGTCGGTGC AGAGGGAGCA TCTGGGCCCG CTGGCGTCCG GCGCCCACAA GCTGGTCCGC 
TTTGCCTCGC AGGGGGCGCC AGCCGGGCTT GGGGAGCCCC AGCTGGAGCT GCACACCCTG 
GACCTTGGGG ACTATGGAGC TCAGGGCGAC TGTGACCCTG AAGCACCAAT GACCGAGGGC 
ACCCGCTGCT GCCGCCAGGA GATGTACATT GACCTGCAGG GGATGAAGTG GGCCGAGAAC 
TGGGTGCTGG AGCCCCCGGG CTTCCTGGCT TATGAGTGTG TGGGCACCTG CCGGCAGCCC 
CCGGAGGCCC TGGCCTTCAA GTGGCCGTTT CTGGGGCCTC GACAGTGCAT CGCCTCGGAG 
ACTGACTCGC TGCCCATGAT CGTCAGCATC AAGGAGGGAG GCAGGACCAG GCCCCAGGTG 
GTCAGCCTGC CCAACATGAG GGTGCAGAAG TGCAGCTGTG CCTCGGATGG TGCGCTCGTG 
CCAAGGAGGC TCCAGCCATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A functional role of LEFTY during progesterone therapy for endometrial carcinoma.
Cell communication and signaling : CCS15(1)56(2017 Dec)
Fei W,Kijima D,Hashimoto M,Hashimura M,Oguri Y,Kajita S,Matsumoto T,Yokoi A,Saegusa M

Lefty inhibits in vitro decidualization by regulating P57 and cyclin D1 expressions.
Cell biochemistry and function32(8)657-64(2014 Dec)
Li H,Li H,Bai L,Yu H

Wnt3a-dependent and -independent protein interaction networks of chromatin-bound ?-catenin in mouse embryonic stem cells.
Molecular & cellular proteomics : MCP12(7)1980-94(2013 Jul)
Yakulov T,Raggioli A,Franz H,Kemler R

Suppression of Lefty expression in induced pluripotent cancer cells.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology27(6)2165-74(2013 Jun)
Saito A,Ochiai H,Okada S,Miyata N,Azuma T

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LEFTY1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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