CENPF cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	CENPF
Entrez Gene ID	1063
Full Name	centromere protein F
Synonyms	CENF,CILD31,PRO1779,STROMS,hcp-1
General protein information	Preferred Names centromere protein F Names centromere protein F AH antigen CENP-F kinetochore protein cell-cycle-dependent 350K nuclear protein centromere protein F, 350/400kDa kinetochore protein CENPF mitosin
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1q41
Summary	This gene encodes a protein that associates with the centromere-kinetochore complex. The protein is a component of the nuclear matrix during the G2 phase of interphase. In late G2 the protein associates with the kinetochore and maintains this association through early anaphase. It localizes to the spindle midzone and the intracellular bridge in late anaphase and telophase, respectively, and is thought to be subsequently degraded. The localization of this protein suggests that it may play a role in chromosome segregation during mitotis. It is thought to form either a homodimer or heterodimer. Autoantibodies against this protein have been found in patients with cancer or graft versus host disease. [provided by RefSeq, Jul 2008].
Disorder MIM:	600236
Disorder Html:	Stromme syndrome, 243605 (3)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017000086.1	XP_016855575.1	centromere protein F isoform X1
NM_016343.4	NP_057427.3	centromere protein F
NM_016343.3	NP_057427.3	centromere protein F
XM_017000086.2	XP_016855575.1	centromere protein F isoform X1
XM_011509082.3	XP_011507384.1	centromere protein F isoform X2
XM_011509082.2	XP_011507384.1	centromere protein F isoform X2

Pathways from BioSystems

Homologies

	CENPF	XP_001105956.2
Xenopus tropicalis (tropical clawed frog)	cenpf	XP_002934738.2
Homo sapiens (human)	CENPF	NP_057427.3
Pan troglodytes (chimpanzee)	CENPF	XP_001171564.1
Canis lupus familiaris (dog)	CENPF	XP_003639228.1
Mus musculus (house mouse)	Cenpf	NP_001074832.2
Bos taurus (cattle)	CENPF	NP_001243515.1
Gallus gallus (chicken)	CENPF	NP_989976.2
Rattus norvegicus (Norway rat)	Cenpf	NP_001094297.1

Gene Ontology

The following CENPF gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CENPF cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu14151	NM_016343.4 Latest version!	Homo sapiens centromere protein F (CENPF), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHu14151	XM_017000086.2 Latest version!	Homo sapiens centromere protein F (CENPF), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHu56821	XM_011509082.3 Latest version!	Homo sapiens centromere protein F (CENPF), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
View Old Accession Versions >>
OHu14151	NM_016343.3	Homo sapiens centromere protein F (CENPF), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHu14151	XM_017000086.1	Homo sapiens centromere protein F (CENPF), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHu56821	XM_011509082.2	Homo sapiens centromere protein F (CENPF), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CENPF ( NM_016343.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_057427.3 Homo sapiens centromere protein F (CENPF), mRNA.

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CloneID	OHu14151
Clone ID Related Accession (Same CDS sequence)	XM_017000086.1 , NM_016343.4 , NM_016343.3 , XM_017000086.2
Accession Version	NM_016343.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9345bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-19
Organism	Homo sapiens(human)
Product	centromere protein F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BU662228.1, U30872.1, BU155312.1, CD654009.1, BG680819.1 and BQ774943.1. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_016343.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U30872.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000366955.8/ ENSP00000355922.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGAGCTGGG CTTTGGAAGA ATGGAAAGAA GGGCTGCCTA CAAGAGCTCT TCAGAAAATT 
CAAGAGCTTG AAGGACAGCT TGACAAACTG AAGAAGGAAA AGCAGCAAAG GCAGTTTCAG 
CTTGACAGTC TCGAGGCTGC GCTGCAGAAG CAAAAACAGA AGGTTGAAAA TGAAAAAACC 
GAGGGTACAA ACCTGAAAAG GGAGAATCAA AGATTGATGG AAATATGTGA AAGTCTGGAG 
AAAACTAAGC AGAAGATTTC TCATGAACTT CAAGTCAAGG AGTCACAAGT GAATTTCCAG 
GAAGGACAAC TGAATTCAGG CAAAAAACAA ATAGAAAAAC TGGAACAGGA ACTTAAAAGG 
TGTAAATCTG AGCTTGAAAG AAGCCAACAA GCTGCGCAGT CTGCAGATGT CTCTCTGAAT 
CCATGCAATA CACCACAAAA AATTTTTACA ACTCCACTAA CACCAAGTCA ATATTATAGT 
GGTTCCAAGT ATGAAGATCT AAAAGAAAAA TATAATAAAG AGGTTGAAGA ACGAAAAAGA 
TTAGAGGCAG AGGTTAAAGC CTTGCAGGCT AAAAAAGCAA GCCAGACTCT TCCACAAGCC 
ACCATGAATC ACCGCGACAT TGCCCGGCAT CAGGCTTCAT CATCTGTGTT CTCATGGCAG 
CAAGAGAAGA CCCCAAGTCA TCTTTCATCT AATTCTCAAA GAACTCCAAT TAGGAGAGAT 
TTCTCTGCAT CTTACTTTTC TGGGGAACAA GAGGTGACTC CAAGTCGATC AACTTTGCAA 
ATAGGGAAAA GAGATGCTAA TAGCAGTTTC TTTGACAATT CTAGCAGTCC TCATCTTTTG 
GATCAATTAA AAGCGCAGAA TCAAGAGCTA AGAAACAAGA TTAATGAGTT GGAACTACGC 
CTGCAAGGAC ATGAAAAAGA AATGAAAGGC CAAGTGAATA AGTTTCAAGA ACTCCAACTC 
CAACTGGAGA AAGCAAAAGT GGAATTAATT GAAAAAGAGA AAGTTTTGAA CAAATGTAGG 
GATGAACTAG TGAGAACAAC AGCACAATAC GACCAGGCGT CAACCAAGTA TACTGCATTG 
GAACAAAAAC TGAAAAAATT GACGGAAGAT TTGAGTTGTC AGCGACAAAA TGCAGAAAGT 
GCCAGATGTT CTCTGGAACA GAAAATTAAG GAAAAAGAAA AGGAGTTTCA AGAGGAGCTC 
TCCCGTCAAC AGCGTTCTTT CCAAACACTG GACCAGGAGT GCATCCAGAT GAAGGCCAGA 
CTCACCCAGG AGTTACAGCA AGCCAAGAAT ATGCACAACG TCCTGCAGGC TGAACTGGAT 
AAACTCACAT CAGTAAAGCA ACAGCTAGAA AACAATTTGG AAGAGTTTAA GCAAAAGTTG 
TGCAGAGCTG AACAGGCGTT CCAGGCGAGT CAGATCAAGG AGAATGAGCT GAGGAGAAGC 
ATGGAGGAAA TGAAGAAGGA AAACAACCTC CTTAAGAGTC ACTCTGAGCA AAAGGCCAGA 
GAAGTCTGCC ACCTGGAGGC AGAACTCAAG AACATCAAAC AGTGTTTAAA TCAGAGCCAG 
AATTTTGCAG AAGAAATGAA AGCGAAGAAT ACCTCTCAGG AAACCATGTT AAGAGATCTT 
CAAGAAAAAA TAAATCAGCA AGAAAACTCC TTGACTTTAG AAAAACTGAA GCTTGCTGTG 
GCTGATCTGG AAAAGCAGCG AGATTGTTCT CAAGACCTTT TGAAGAAAAG AGAACATCAC 
ATTGAACAAC TTAATGATAA GTTAAGCAAG ACAGAGAAAG AGTCCAAAGC CTTGCTGAGT 
GCTTTAGAGT TAAAAAAGAA AGAATATGAA GAATTGAAAG AAGAGAAAAC TCTGTTTTCT 
TGTTGGAAAA GTGAAAACGA AAAACTTTTA ACTCAGATGG AATCAGAAAA GGAAAACTTG 
CAGAGTAAAA TTAATCACTT GGAAACTTGT CTGAAGACAC AGCAAATAAA AAGTCATGAA 
TACAACGAGA GAGTAAGAAC GCTGGAGATG GACAGAGAAA ACCTAAGTGT CGAGATCAGA 
AACCTTCACA ACGTGTTAGA CAGTAAGTCA GTGGAGGTAG AGACCCAGAA ACTAGCTTAT 
ATGGAGCTAC AGCAGAAAGC TGAGTTCTCA GATCAGAAAC ATCAGAAGGA AATAGAAAAT 
ATGTGTTTGA AGACTTCTCA GCTTACTGGG CAAGTTGAAG ATCTAGAACA CAAGCTTCAG 
TTACTGTCAA ATGAAATAAT GGACAAAGAC CGGTGTTACC AAGACTTGCA TGCCGAATAT 
GAGAGCCTCA GGGATCTGCT AAAATCCAAA GATGCTTCTC TGGTGACAAA TGAAGATCAT 
CAGAGAAGTC TTTTGGCTTT TGATCAGCAG CCTGCCATGC ATCATTCCTT TGCAAATATA 
ATTGGAGAAC AAGGAAGCAT GCCTTCAGAG AGGAGTGAAT GTCGTTTAGA AGCAGACCAA 
AGTCCGAAAA ATTCTGCCAT CCTACAAAAT AGAGTTGATT CACTTGAATT TTCATTAGAG 
TCTCAAAAAC AGATGAACTC AGACCTGCAA AAGCAGTGTG AAGAGTTGGT GCAAATCAAA 
GGAGAAATAG AAGAAAATCT CATGAAAGCA GAACAGATGC ATCAAAGTTT TGTGGCTGAA 
ACAAGTCAGC GCATTAGTAA GTTACAGGAA GACACTTCTG CTCACCAGAA TGTTGTTGCT 
GAAACCTTAA GTGCCCTTGA GAACAAGGAA AAAGAGCTGC AACTTTTAAA TGATAAGGTA 
GAAACTGAGC AGGCAGAGAT TCAAGAATTA AAAAAGAGCA ACCATCTACT TGAAGACTCT 
CTAAAGGAGC TACAACTTTT ATCCGAAACC CTAAGCTTGG AGAAGAAAGA AATGAGTTCC 
ATCATTTCTC TAAATAAAAG GGAAATTGAA GAGCTGACCC AAGAGAATGG GACTCTTAAG 
GAAATTAATG CATCCTTAAA TCAAGAGAAG ATGAACTTAA TCCAGAAAAG TGAGAGTTTT 
GCAAACTATA TAGATGAAAG GGAGAAAAGC ATTTCAGAGT TATCTGATCA GTACAAGCAA 
GAAAAACTTA TTTTACTACA AAGATGTGAA GAAACCGGAA ATGCATATGA GGATCTTAGT 
CAAAAATACA AAGCAGCACA GGAAAAGAAT TCTAAATTAG AATGCTTGCT AAATGAATGC 
ACTAGTCTTT GTGAAAATAG GAAAAATGAG TTGGAACAGC TAAAGGAAGC ATTTGCAAAG 
GAACACCAAG AATTCTTAAC AAAATTAGCA TTTGCTGAAG AAAGAAATCA GAATCTGATG 
CTAGAGTTGG AGACAGTGCA GCAAGCTCTG AGATCTGAGA TGACAGATAA CCAAAACAAT 
TCTAAGAGCG AGGCTGGTGG TTTAAAGCAA GAAATCATGA CTTTAAAGGA AGAACAAAAC 
AAAATGCAAA AGGAAGTTAA TGACTTATTA CAAGAGAATG AACAGCTGAT GAAGGTAATG 
AAGACTAAAC ATGAATGTCA AAATCTAGAA TCAGAACCAA TTAGGAACTC TGTGAAAGAA 
AGAGAGAGTG AGAGAAATCA ATGTAATTTT AAACCTCAGA TGGATCTTGA AGTTAAAGAA 
ATTTCTCTAG ATAGTTATAA TGCGCAGTTG GTGCAATTAG AAGCTATGCT AAGAAATAAG 
GAATTAAAAC TTCAGGAAAG TGAGAAGGAG AAGGAGTGCC TGCAGCATGA ATTACAGACA 
ATTAGAGGAG ATCTTGAAAC CAGCAATTTG CAAGACATGC AGTCACAAGA AATTAGTGGC 
CTTAAAGACT GTGAAATAGA TGCGGAAGAA AAGTATATTT CAGGGCCTCA TGAGTTGTCA 
ACAAGTCAAA ACGACAATGC ACACCTTCAG TGCTCTCTGC AAACAACAAT GAACAAGCTG 
AATGAGCTAG AGAAAATATG TGAAATACTG CAGGCTGAAA AGTATGAACT CGTAACTGAG 
CTGAATGATT CAAGGTCAGA ATGTATCACA GCAACTAGGA AAATGGCAGA AGAGGTAGGG 
AAACTACTAA ATGAAGTTAA AATATTAAAT GATGACAGTG GTCTTCTCCA TGGTGAGTTA 
GTGGAAGACA TACCAGGAGG TGAATTTGGT GAACAACCAA ATGAACAGCA CCCTGTGTCT 
TTGGCTCCAT TGGACGAGAG TAATTCCTAC GAGCACTTGA CATTGTCAGA CAAAGAAGTT 
CAAATGCACT TTGCCGAATT GCAAGAGAAA TTCTTATCTT TACAAAGTGA ACACAAAATT 
TTACATGATC AGCACTGTCA GATGAGCTCT AAAATGTCAG AGCTGCAGAC CTATGTTGAC 
TCATTAAAGG CCGAAAATTT GGTCTTGTCA ACGAATCTGA GAAACTTTCA AGGTGACTTG 
GTGAAGGAGA TGCAGCTGGG CTTGGAGGAG GGGCTCGTTC CATCCCTGTC ATCCTCTTGT 
GTGCCTGACA GCTCTAGTCT TAGCAGTTTG GGAGACTCCT CCTTTTACAG AGCTCTTTTA 
GAACAGACAG GAGATATGTC TCTTTTGAGT AATTTAGAAG GGGCTGTTTC AGCAAACCAG 
TGCAGTGTAG ATGAAGTATT TTGCAGCAGT CTGCAGGAGG AGAATCTGAC CAGGAAAGAA 
ACCCCTTCGG CCCCAGCGAA GGGTGTTGAA GAGCTTGAGT CCCTCTGTGA GGTGTACCGG 
CAGTCCCTCG AGAAGCTAGA AGAGAAAATG GAAAGTCAAG GGATTATGAA AAATAAGGAA 
ATTCAAGAGC TCGAGCAGTT ATTAAGTTCT GAAAGGCAAG AGCTTGACTG CCTTAGGAAG 
CAGTATTTGT CAGAAAATGA ACAGTGGCAA CAGAAGCTGA CAAGCGTGAC TCTGGAGATG 
GAGTCCAAGT TGGCGGCAGA AAAGAAACAG ACGGAACAAC TGTCACTTGA GCTGGAAGTA 
GCACGACTCC AGCTACAAGG TCTGGACTTA AGTTCTCGGT CTTTGCTTGG CATCGACACA 
GAAGATGCTA TTCAAGGCCG AAATGAGAGC TGTGACATAT CAAAAGAACA TACTTCAGAA 
ACTACAGAAA GAACACCAAA GCATGATGTT CATCAGATTT GTGATAAAGA TGCTCAGCAG 
GACCTCAATC TAGACATTGA GAAAATAACT GAGACTGGTG CAGTGAAACC CACAGGAGAG 
TGCTCTGGGG AACAGTCCCC AGATACCAAT TATGAGCCTC CAGGGGAAGA TAAAACCCAG 
GGCTCTTCAG AATGCATTTC TGAATTGTCA TTTTCTGGTC CTAATGCTTT GGTACCTATG 
GATTTCCTGG GGAATCAGGA AGATATCCAT AATCTTCAAC TGCGGGTAAA AGAGACATCA 
AATGAGAATT TGAGATTACT TCATGTGATA GAGGACCGTG ACAGAAAAGT TGAAAGTTTG 
CTAAATGAAA TGAAAGAATT AGACTCAAAA CTCCATTTAC AGGAGGTACA ACTAATGACC 
AAAATTGAAG CATGCATAGA ATTGGAAAAA ATAGTTGGGG AACTTAAGAA AGAAAACTCA 
GATTTAAGTG AAAAATTGGA ATATTTTTCT TGTGATCACC AGGAGTTACT CCAGAGAGTA 
GAAACTTCTG AAGGCCTCAA TTCTGATTTA GAAATGCATG CAGATAAATC ATCACGTGAA 
GATATTGGAG ATAATGTGGC CAAGGTGAAT GACAGCTGGA AGGAGAGATT TCTTGATGTG 
GAAAATGAGC TGAGTAGGAT CAGATCGGAG AAAGCTAGCA TTGAGCATGA AGCCCTCTAC 
CTGGAGGCTG ACTTAGAGGT AGTTCAAACA GAGAAGCTAT GTTTAGAAAA AGACAATGAA 
AATAAGCAGA AGGTTATTGT CTGCCTTGAA GAAGAACTCT CAGTGGTCAC AAGTGAGAGA 
AACCAGCTTC GTGGAGAATT AGATACTATG TCAAAAAAAA CCACGGCACT GGATCAGTTG 
TCTGAAAAAA TGAAGGAGAA AACACAAGAG CTTGAGTCTC ATCAAAGTGA GTGTCTCCAT 
TGCATTCAGG TGGCAGAGGC AGAGGTGAAG GAAAAGACGG AACTCCTTCA GACTTTGTCC 
TCTGATGTGA GTGAGCTGTT AAAAGACAAA ACTCATCTCC AGGAAAAGCT GCAGAGTTTG 
GAAAAGGACT CACAGGCACT GTCTTTGACA AAATGTGAGC TGGAAAACCA AATTGCACAA 
CTGAATAAAG AGAAAGAATT GCTTGTCAAG GAATCTGAAA GCCTGCAGGC CAGACTGAGT 
GAATCAGATT ATGAAAAGCT GAATGTCTCC AAGGCCTTGG AGGCCGCACT GGTGGAGAAA 
GGTGAGTTCG CATTGAGGCT GAGCTCAACA CAGGAGGAAG TGCATCAGCT GAGAAGAGGC 
ATCGAGAAAC TGAGAGTTCG CATTGAGGCC GATGAAAAGA AGCAGCTGCA CATCGCAGAG 
AAACTGAAAG AACGCGAGCG GGAGAATGAT TCACTTAAGG ATAAAGTTGA GAACCTTGAA 
AGGGAATTGC AGATGTCAGA AGAAAACCAG GAGCTAGTGA TTCTTGATGC CGAGAATTCC 
AAAGCAGAAG TAGAGACTCT AAAAACACAA ATAGAAGAGA TGGCCAGAAG CCTGAAAGTT 
TTTGAATTAG ACCTTGTCAC GTTAAGGTCT GAAAAAGAAA ATCTGACAAA ACAAATACAA 
GAAAAACAAG GTCAGTTGTC AGAACTAGAC AAGTTACTCT CTTCATTTAA AAGTCTGTTA 
GAAGAAAAGG AGCAAGCAGA GATACAGATC AAAGAAGAAT CTAAAACTGC AGTGGAGATG 
CTTCAGAATC AGTTAAAGGA GCTAAATGAG GCAGTAGCAG CCTTGTGTGG TGACCAAGAA 
ATTATGAAGG CCACAGAACA GAGTCTAGAC CCACCAATAG AGGAAGAGCA TCAGCTGAGA 
AATAGCATTG AAAAGCTGAG AGCCCGCCTA GAAGCTGATG AAAAGAAGCA GCTCTGTGTC 
TTACAACAAC TGAAGGAAAG TGAGCATCAT GCAGATTTAC TTAAGGGTAG AGTGGAGAAC 
CTTGAAAGAG AGCTAGAGAT AGCCAGGACA AACCAAGAGC ATGCAGCTCT TGAGGCAGAG 
AATTCCAAAG GAGAGGTAGA GACCCTAAAA GCAAAAATAG AAGGGATGAC CCAAAGTCTG 
AGAGGTCTGG AATTAGATGT TGTTACTATA AGGTCAGAAA AAGAAAATCT GACAAATGAA 
TTACAAAAAG AGCAAGAGCG AATATCTGAA TTAGAAATAA TAAATTCATC ATTTGAAAAT 
ATTTTGCAAG AAAAAGAGCA AGAGAAAGTA CAGATGAAAG AAAAATCAAG CACTGCCATG 
GAGATGCTTC AAACACAATT AAAAGAGCTC AATGAGAGAG TGGCAGCCCT GCATAATGAC 
CAAGAAGCCT GTAAGGCCAA AGAGCAGAAT CTTAGTAGTC AAGTAGAGTG TCTTGAACTT 
GAGAAGGCTC AGTTGCTACA AGGCCTTGAT GAGGCCAAAA ATAATTATAT TGTTTTGCAA 
TCTTCAGTGA ATGGCCTCAT TCAAGAAGTA GAAGATGGCA AGCAGAAACT GGAGAAGAAG 
GATGAAGAAA TCAGTAGACT GAAAAATCAA ATTCAAGACC AAGAGCAGCT TGTCTCTAAA 
CTGTCCCAGG TGGAAGGAGA GCACCAACTT TGGAAGGAGC AAAACTTAGA ACTGAGAAAT 
CTGACAGTGG AATTGGAGCA GAAGATCCAA GTGCTACAAT CCAAAAATGC CTCTTTGCAG 
GACACATTAG AAGTGCTGCA GAGTTCTTAC AAGAATCTAG AGAATGAGCT TGAATTGACA 
AAAATGGACA AAATGTCCTT TGTTGAAAAA GTAAACAAAA TGACTGCAAA GGAAACTGAG 
CTGCAGAGGG AAATGCATGA GATGGCACAG AAAACAGCAG AGCTGCAAGA AGAACTCAGT 
GGAGAGAAAA ATAGGCTAGC TGGAGAGTTG CAGTTACTGT TGGAAGAAAT AAAGAGCAGC 
AAAGATCAAT TGAAGGAGCT CACACTAGAA AATAGTGAAT TGAAGAAGAG CCTAGATTGC 
ATGCACAAAG ACCAGGTGGA AAAGGAAGGG AAAGTGAGAG AGGAAATAGC TGAATATCAG 
CTACGGCTTC ATGAAGCTGA AAAGAAACAC CAGGCTTTGC TTTTGGACAC AAACAAACAG 
TATGAAGTAG AAATCCAGAC ATACCGAGAG AAATTGACTT CTAAAGAAGA ATGTCTCAGT 
TCACAGAAGC TGGAGATAGA CCTTTTAAAG TCTAGTAAAG AAGAGCTCAA TAATTCATTG 
AAAGCTACTA CTCAGATTTT GGAAGAATTG AAGAAAACCA AGATGGACAA TCTAAAATAT 
GTAAATCAGT TGAAGAAGGA AAATGAACGT GCCCAGGGGA AAATGAAGTT GTTGATCAAA 
TCCTGTAAAC AGCTGGAAGA GGAAAAGGAG ATACTGCAGA AAGAACTCTC TCAACTTCAA 
GCTGCACAGG AGAAGCAGAA AACAGGTACT GTTATGGATA CCAAGGTCGA TGAATTAACA 
ACTGAGATCA AAGAACTGAA AGAAACTCTT GAAGAAAAAA CCAAGGAGGC AGATGAATAC 
TTGGATAAGT ACTGTTCCTT GCTTATAAGC CATGAAAAGT TAGAGAAAGC TAAAGAGATG 
TTAGAGACAC AAGTGGCCCA TCTGTGTTCA CAGCAATCTA AACAAGATTC CCGAGGGTCT 
CCTTTGCTAG GTCCAGTTGT TCCAGGACCA TCTCCAATCC CTTCTGTTAC TGAAAAGAGG 
TTATCATCTG GCCAAAATAA AGCTTCAGGC AAGAGGCAAA GATCCAGTGG AATATGGGAG 
AATGGTAGAG GACCAACACC TGCTACCCCA GAGAGCTTTT CTAAAAAAAG CAAGAAAGCA 
GTCATGAGTG GTATTCACCC TGCAGAAGAC ACGGAAGGTA CTGAGTTTGA GCCAGAGGGA 
CTTCCAGAAG TTGTAAAGAA AGGGTTTGCT GACATCCCGA CAGGAAAGAC TAGCCCATAT 
ATCCTGCGAA GAACAACCAT GGCAACTCGG ACCAGCCCCC GCCTGGCTGC ACAGAAGTTA 
GCGCTATCCC CACTGAGTCT CGGCAAAGAA AATCTTGCAG AGTCCTCCAA ACCAACAGCT 
GGTGGCAGCA GATCACAAAA GGTCAAAGTT GCTCAGCGGA GCCCAGTAGA TTCAGGCACC 
ATCCTCCGAG AACCCACCAC GAAATCCGTC CCAGTCAATA ATCTTCCTGA GAGAAGTCCG 
ACTGACAGCC CCAGAGAGGG CCTGAGGGTC AAGCGAGGCC GACTTGTCCC CAGCCCCAAA 
GCTGGACTGG AGTCCAACGG CAGTGAGAAC TGTAAGGTCC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_057427.3
CDS	169..9513
Translation	MSWALEEWKEGLPTRALQKIQELEGQLDKLKKEKQQRQFQLDSLEAALQKQKQKVENEKTEGTNLKRENQRLMEICESLEKTKQKISHELQVKESQVNFQEGQLNSGKKQIEKLEQELKRCKSELERSQQAAQSADVSLNPCNTPQKIFTTPLTPSQYYSGSKYEDLKEKYNKEVEERKRLEAEVKALQAKKASQTLPQATMNHRDIARHQASSSVFSWQQEKTPSHLSSNSQRTPIRRDFSASYFSGEQEVTPSRSTLQIGKRDANSSFFDNSSSPHLLDQLKAQNQELRNKINELELRLQGHEKEMKGQVNKFQELQLQLEKAKVELIEKEKVLNKCRDELVRTTAQYDQASTKYTALEQKLKKLTEDLSCQRQNAESARCSLEQKIKEKEKEFQEELSRQQRSFQTLDQECIQMKARLTQELQQAKNMHNVLQAELDKLTSVKQQLENNLEEFKQKLCRAEQAFQASQIKENELRRSMEEMKKENNLLKSHSEQKAREVCHLEAELKNIKQCLNQSQNFAEEMKAKNTSQETMLRDLQEKINQQENSLTLEKLKLAVADLEKQRDCSQDLLKKREHHIEQLNDKLSKTEKESKALLSALELKKKEYEELKEEKTLFSCWKSENEKLLTQMESEKENLQSKINHLETCLKTQQIKSHEYNERVRTLEMDRENLSVEIRNLHNVLDSKSVEVETQKLAYMELQQKAEFSDQKHQKEIENMCLKTSQLTGQVEDLEHKLQLLSNEIMDKDRCYQDLHAEYESLRDLLKSKDASLVTNEDHQRSLLAFDQQPAMHHSFANIIGEQGSMPSERSECRLEADQSPKNSAILQNRVDSLEFSLESQKQMNSDLQKQCEELVQIKGEIEENLMKAEQMHQSFVAETSQRISKLQEDTSAHQNVVAETLSALENKEKELQLLNDKVETEQAEIQELKKSNHLLEDSLKELQLLSETLSLEKKEMSSIISLNKREIEELTQENGTLKEINASLNQEKMNLIQKSESFANYIDEREKSISELSDQYKQEKLILLQRCEETGNAYEDLSQKYKAAQEKNSKLECLLNECTSLCENRKNELEQLKEAFAKEHQEFLTKLAFAEERNQNLMLELETVQQALRSEMTDNQNNSKSEAGGLKQEIMTLKEEQNKMQKEVNDLLQENEQLMKVMKTKHECQNLESEPIRNSVKERESERNQCNFKPQMDLEVKEISLDSYNAQLVQLEAMLRNKELKLQESEKEKECLQHELQTIRGDLETSNLQDMQSQEISGLKDCEIDAEEKYISGPHELSTSQNDNAHLQCSLQTTMNKLNELEKICEILQAEKYELVTELNDSRSECITATRKMAEEVGKLLNEVKILNDDSGLLHGELVEDIPGGEFGEQPNEQHPVSLAPLDESNSYEHLTLSDKEVQMHFAELQEKFLSLQSEHKILHDQHCQMSSKMSELQTYVDSLKAENLVLSTNLRNFQGDLVKEMQLGLEEGLVPSLSSSCVPDSSSLSSLGDSSFYRALLEQTGDMSLLSNLEGAVSANQCSVDEVFCSSLQEENLTRKETPSAPAKGVEELESLCEVYRQSLEKLEEKMESQGIMKNKEIQELEQLLSSERQELDCLRKQYLSENEQWQQKLTSVTLEMESKLAAEKKQTEQLSLELEVARLQLQGLDLSSRSLLGIDTEDAIQGRNESCDISKEHTSETTERTPKHDVHQICDKDAQQDLNLDIEKITETGAVKPTGECSGEQSPDTNYEPPGEDKTQGSSECISELSFSGPNALVPMDFLGNQEDIHNLQLRVKETSNENLRLLHVIEDRDRKVESLLNEMKELDSKLHLQEVQLMTKIEACIELEKIVGELKKENSDLSEKLEYFSCDHQELLQRVETSEGLNSDLEMHADKSSREDIGDNVAKVNDSWKERFLDVENELSRIRSEKASIEHEALYLEADLEVVQTEKLCLEKDNENKQKVIVCLEEELSVVTSERNQLRGELDTMSKKTTALDQLSEKMKEKTQELESHQSECLHCIQVAEAEVKEKTELLQTLSSDVSELLKDKTHLQEKLQSLEKDSQALSLTKCELENQIAQLNKEKELLVKESESLQARLSESDYEKLNVSKALEAALVEKGEFALRLSSTQEEVHQLRRGIEKLRVRIEADEKKQLHIAEKLKERERENDSLKDKVENLERELQMSEENQELVILDAENSKAEVETLKTQIEEMARSLKVFELDLVTLRSEKENLTKQIQEKQGQLSELDKLLSSFKSLLEEKEQAEIQIKEESKTAVEMLQNQLKELNEAVAALCGDQEIMKATEQSLDPPIEEEHQLRNSIEKLRARLEADEKKQLCVLQQLKESEHHADLLKGRVENLERELEIARTNQEHAALEAENSKGEVETLKAKIEGMTQSLRGLELDVVTIRSEKENLTNELQKEQERISELEIINSSFENILQEKEQEKVQMKEKSSTAMEMLQTQLKELNERVAALHNDQEACKAKEQNLSSQVECLELEKAQLLQGLDEAKNNYIVLQSSVNGLIQEVEDGKQKLEKKDEEISRLKNQIQDQEQLVSKLSQVEGEHQLWKEQNLELRNLTVELEQKIQVLQSKNASLQDTLEVLQSSYKNLENELELTKMDKMSFVEKVNKMTAKETELQREMHEMAQKTAELQEELSGEKNRLAGELQLLLEEIKSSKDQLKELTLENSELKKSLDCMHKDQVEKEGKVREEIAEYQLRLHEAEKKHQALLLDTNKQYEVEIQTYREKLTSKEECLSSQKLEIDLLKSSKEELNNSLKATTQILEELKKTKMDNLKYVNQLKKENERAQGKMKLLIKSCKQLEEEKEILQKELSQLQAAQEKQKTGTVMDTKVDELTTEIKELKETLEEKTKEADEYLDKYCSLLISHEKLEKAKEMLETQVAHLCSQQSKQDSRGSPLLGPVVPGPSPIPSVTEKRLSSGQNKASGKRQRSSGIWENGRGPTPATPESFSKKSKKAVMSGIHPAEDTEGTEFEPEGLPEVVKKGFADIPTGKTSPYILRRTTMATRTSPRLAAQKLALSPLSLGKENLAESSKPTAGGSRSQKVKVAQRSPVDSGTILREPTTKSVPVNNLPERSPTDSPREGLRVKRGRLVPSPKAGLESNGSENCKVQ

Target ORF information:

RefSeq Version	NM_016343.4
Organism	Homo sapiens(human)
Definition	Homo sapiens centromere protein F (CENPF), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_016343.4

ORF Insert Sequence:

ATGAGCTGGG CTTTGGAAGA ATGGAAAGAA GGGCTGCCTA CAAGAGCTCT TCAGAAAATT 
CAAGAGCTTG AAGGACAGCT TGACAAACTG AAGAAGGAAA AGCAGCAAAG GCAGTTTCAG 
CTTGACAGTC TCGAGGCTGC GCTGCAGAAG CAAAAACAGA AGGTTGAAAA TGAAAAAACC 
GAGGGTACAA ACCTGAAAAG GGAGAATCAA AGATTGATGG AAATATGTGA AAGTCTGGAG 
AAAACTAAGC AGAAGATTTC TCATGAACTT CAAGTCAAGG AGTCACAAGT GAATTTCCAG 
GAAGGACAAC TGAATTCAGG CAAAAAACAA ATAGAAAAAC TGGAACAGGA ACTTAAAAGG 
TGTAAATCTG AGCTTGAAAG AAGCCAACAA GCTGCGCAGT CTGCAGATGT CTCTCTGAAT 
CCATGCAATA CACCACAAAA AATTTTTACA ACTCCACTAA CACCAAGTCA ATATTATAGT 
GGTTCCAAGT ATGAAGATCT AAAAGAAAAA TATAATAAAG AGGTTGAAGA ACGAAAAAGA 
TTAGAGGCAG AGGTTAAAGC CTTGCAGGCT AAAAAAGCAA GCCAGACTCT TCCACAAGCC 
ACCATGAATC ACCGCGACAT TGCCCGGCAT CAGGCTTCAT CATCTGTGTT CTCATGGCAG 
CAAGAGAAGA CCCCAAGTCA TCTTTCATCT AATTCTCAAA GAACTCCAAT TAGGAGAGAT 
TTCTCTGCAT CTTACTTTTC TGGGGAACAA GAGGTGACTC CAAGTCGATC AACTTTGCAA 
ATAGGGAAAA GAGATGCTAA TAGCAGTTTC TTTGACAATT CTAGCAGTCC TCATCTTTTG 
GATCAATTAA AAGCGCAGAA TCAAGAGCTA AGAAACAAGA TTAATGAGTT GGAACTACGC 
CTGCAAGGAC ATGAAAAAGA AATGAAAGGC CAAGTGAATA AGTTTCAAGA ACTCCAACTC 
CAACTGGAGA AAGCAAAAGT GGAATTAATT GAAAAAGAGA AAGTTTTGAA CAAATGTAGG 
GATGAACTAG TGAGAACAAC AGCACAATAC GACCAGGCGT CAACCAAGTA TACTGCATTG 
GAACAAAAAC TGAAAAAATT GACGGAAGAT TTGAGTTGTC AGCGACAAAA TGCAGAAAGT 
GCCAGATGTT CTCTGGAACA GAAAATTAAG GAAAAAGAAA AGGAGTTTCA AGAGGAGCTC 
TCCCGTCAAC AGCGTTCTTT CCAAACACTG GACCAGGAGT GCATCCAGAT GAAGGCCAGA 
CTCACCCAGG AGTTACAGCA AGCCAAGAAT ATGCACAACG TCCTGCAGGC TGAACTGGAT 
AAACTCACAT CAGTAAAGCA ACAGCTAGAA AACAATTTGG AAGAGTTTAA GCAAAAGTTG 
TGCAGAGCTG AACAGGCGTT CCAGGCGAGT CAGATCAAGG AGAATGAGCT GAGGAGAAGC 
ATGGAGGAAA TGAAGAAGGA AAACAACCTC CTTAAGAGTC ACTCTGAGCA AAAGGCCAGA 
GAAGTCTGCC ACCTGGAGGC AGAACTCAAG AACATCAAAC AGTGTTTAAA TCAGAGCCAG 
AATTTTGCAG AAGAAATGAA AGCGAAGAAT ACCTCTCAGG AAACCATGTT AAGAGATCTT 
CAAGAAAAAA TAAATCAGCA AGAAAACTCC TTGACTTTAG AAAAACTGAA GCTTGCTGTG 
GCTGATCTGG AAAAGCAGCG AGATTGTTCT CAAGACCTTT TGAAGAAAAG AGAACATCAC 
ATTGAACAAC TTAATGATAA GTTAAGCAAG ACAGAGAAAG AGTCCAAAGC CTTGCTGAGT 
GCTTTAGAGT TAAAAAAGAA AGAATATGAA GAATTGAAAG AAGAGAAAAC TCTGTTTTCT 
TGTTGGAAAA GTGAAAACGA AAAACTTTTA ACTCAGATGG AATCAGAAAA GGAAAACTTG 
CAGAGTAAAA TTAATCACTT GGAAACTTGT CTGAAGACAC AGCAAATAAA AAGTCATGAA 
TACAACGAGA GAGTAAGAAC GCTGGAGATG GACAGAGAAA ACCTAAGTGT CGAGATCAGA 
AACCTTCACA ACGTGTTAGA CAGTAAGTCA GTGGAGGTAG AGACCCAGAA ACTAGCTTAT 
ATGGAGCTAC AGCAGAAAGC TGAGTTCTCA GATCAGAAAC ATCAGAAGGA AATAGAAAAT 
ATGTGTTTGA AGACTTCTCA GCTTACTGGG CAAGTTGAAG ATCTAGAACA CAAGCTTCAG 
TTACTGTCAA ATGAAATAAT GGACAAAGAC CGGTGTTACC AAGACTTGCA TGCCGAATAT 
GAGAGCCTCA GGGATCTGCT AAAATCCAAA GATGCTTCTC TGGTGACAAA TGAAGATCAT 
CAGAGAAGTC TTTTGGCTTT TGATCAGCAG CCTGCCATGC ATCATTCCTT TGCAAATATA 
ATTGGAGAAC AAGGAAGCAT GCCTTCAGAG AGGAGTGAAT GTCGTTTAGA AGCAGACCAA 
AGTCCGAAAA ATTCTGCCAT CCTACAAAAT AGAGTTGATT CACTTGAATT TTCATTAGAG 
TCTCAAAAAC AGATGAACTC AGACCTGCAA AAGCAGTGTG AAGAGTTGGT GCAAATCAAA 
GGAGAAATAG AAGAAAATCT CATGAAAGCA GAACAGATGC ATCAAAGTTT TGTGGCTGAA 
ACAAGTCAGC GCATTAGTAA GTTACAGGAA GACACTTCTG CTCACCAGAA TGTTGTTGCT 
GAAACCTTAA GTGCCCTTGA GAACAAGGAA AAAGAGCTGC AACTTTTAAA TGATAAGGTA 
GAAACTGAGC AGGCAGAGAT TCAAGAATTA AAAAAGAGCA ACCATCTACT TGAAGACTCT 
CTAAAGGAGC TACAACTTTT ATCCGAAACC CTAAGCTTGG AGAAGAAAGA AATGAGTTCC 
ATCATTTCTC TAAATAAAAG GGAAATTGAA GAGCTGACCC AAGAGAATGG GACTCTTAAG 
GAAATTAATG CATCCTTAAA TCAAGAGAAG ATGAACTTAA TCCAGAAAAG TGAGAGTTTT 
GCAAACTATA TAGATGAAAG GGAGAAAAGC ATTTCAGAGT TATCTGATCA GTACAAGCAA 
GAAAAACTTA TTTTACTACA AAGATGTGAA GAAACCGGAA ATGCATATGA GGATCTTAGT 
CAAAAATACA AAGCAGCACA GGAAAAGAAT TCTAAATTAG AATGCTTGCT AAATGAATGC 
ACTAGTCTTT GTGAAAATAG GAAAAATGAG TTGGAACAGC TAAAGGAAGC ATTTGCAAAG 
GAACACCAAG AATTCTTAAC AAAATTAGCA TTTGCTGAAG AAAGAAATCA GAATCTGATG 
CTAGAGTTGG AGACAGTGCA GCAAGCTCTG AGATCTGAGA TGACAGATAA CCAAAACAAT 
TCTAAGAGCG AGGCTGGTGG TTTAAAGCAA GAAATCATGA CTTTAAAGGA AGAACAAAAC 
AAAATGCAAA AGGAAGTTAA TGACTTATTA CAAGAGAATG AACAGCTGAT GAAGGTAATG 
AAGACTAAAC ATGAATGTCA AAATCTAGAA TCAGAACCAA TTAGGAACTC TGTGAAAGAA 
AGAGAGAGTG AGAGAAATCA ATGTAATTTT AAACCTCAGA TGGATCTTGA AGTTAAAGAA 
ATTTCTCTAG ATAGTTATAA TGCGCAGTTG GTGCAATTAG AAGCTATGCT AAGAAATAAG 
GAATTAAAAC TTCAGGAAAG TGAGAAGGAG AAGGAGTGCC TGCAGCATGA ATTACAGACA 
ATTAGAGGAG ATCTTGAAAC CAGCAATTTG CAAGACATGC AGTCACAAGA AATTAGTGGC 
CTTAAAGACT GTGAAATAGA TGCGGAAGAA AAGTATATTT CAGGGCCTCA TGAGTTGTCA 
ACAAGTCAAA ACGACAATGC ACACCTTCAG TGCTCTCTGC AAACAACAAT GAACAAGCTG 
AATGAGCTAG AGAAAATATG TGAAATACTG CAGGCTGAAA AGTATGAACT CGTAACTGAG 
CTGAATGATT CAAGGTCAGA ATGTATCACA GCAACTAGGA AAATGGCAGA AGAGGTAGGG 
AAACTACTAA ATGAAGTTAA AATATTAAAT GATGACAGTG GTCTTCTCCA TGGTGAGTTA 
GTGGAAGACA TACCAGGAGG TGAATTTGGT GAACAACCAA ATGAACAGCA CCCTGTGTCT 
TTGGCTCCAT TGGACGAGAG TAATTCCTAC GAGCACTTGA CATTGTCAGA CAAAGAAGTT 
CAAATGCACT TTGCCGAATT GCAAGAGAAA TTCTTATCTT TACAAAGTGA ACACAAAATT 
TTACATGATC AGCACTGTCA GATGAGCTCT AAAATGTCAG AGCTGCAGAC CTATGTTGAC 
TCATTAAAGG CCGAAAATTT GGTCTTGTCA ACGAATCTGA GAAACTTTCA AGGTGACTTG 
GTGAAGGAGA TGCAGCTGGG CTTGGAGGAG GGGCTCGTTC CATCCCTGTC ATCCTCTTGT 
GTGCCTGACA GCTCTAGTCT TAGCAGTTTG GGAGACTCCT CCTTTTACAG AGCTCTTTTA 
GAACAGACAG GAGATATGTC TCTTTTGAGT AATTTAGAAG GGGCTGTTTC AGCAAACCAG 
TGCAGTGTAG ATGAAGTATT TTGCAGCAGT CTGCAGGAGG AGAATCTGAC CAGGAAAGAA 
ACCCCTTCGG CCCCAGCGAA GGGTGTTGAA GAGCTTGAGT CCCTCTGTGA GGTGTACCGG 
CAGTCCCTCG AGAAGCTAGA AGAGAAAATG GAAAGTCAAG GGATTATGAA AAATAAGGAA 
ATTCAAGAGC TCGAGCAGTT ATTAAGTTCT GAAAGGCAAG AGCTTGACTG CCTTAGGAAG 
CAGTATTTGT CAGAAAATGA ACAGTGGCAA CAGAAGCTGA CAAGCGTGAC TCTGGAGATG 
GAGTCCAAGT TGGCGGCAGA AAAGAAACAG ACGGAACAAC TGTCACTTGA GCTGGAAGTA 
GCACGACTCC AGCTACAAGG TCTGGACTTA AGTTCTCGGT CTTTGCTTGG CATCGACACA 
GAAGATGCTA TTCAAGGCCG AAATGAGAGC TGTGACATAT CAAAAGAACA TACTTCAGAA 
ACTACAGAAA GAACACCAAA GCATGATGTT CATCAGATTT GTGATAAAGA TGCTCAGCAG 
GACCTCAATC TAGACATTGA GAAAATAACT GAGACTGGTG CAGTGAAACC CACAGGAGAG 
TGCTCTGGGG AACAGTCCCC AGATACCAAT TATGAGCCTC CAGGGGAAGA TAAAACCCAG 
GGCTCTTCAG AATGCATTTC TGAATTGTCA TTTTCTGGTC CTAATGCTTT GGTACCTATG 
GATTTCCTGG GGAATCAGGA AGATATCCAT AATCTTCAAC TGCGGGTAAA AGAGACATCA 
AATGAGAATT TGAGATTACT TCATGTGATA GAGGACCGTG ACAGAAAAGT TGAAAGTTTG 
CTAAATGAAA TGAAAGAATT AGACTCAAAA CTCCATTTAC AGGAGGTACA ACTAATGACC 
AAAATTGAAG CATGCATAGA ATTGGAAAAA ATAGTTGGGG AACTTAAGAA AGAAAACTCA 
GATTTAAGTG AAAAATTGGA ATATTTTTCT TGTGATCACC AGGAGTTACT CCAGAGAGTA 
GAAACTTCTG AAGGCCTCAA TTCTGATTTA GAAATGCATG CAGATAAATC ATCACGTGAA 
GATATTGGAG ATAATGTGGC CAAGGTGAAT GACAGCTGGA AGGAGAGATT TCTTGATGTG 
GAAAATGAGC TGAGTAGGAT CAGATCGGAG AAAGCTAGCA TTGAGCATGA AGCCCTCTAC 
CTGGAGGCTG ACTTAGAGGT AGTTCAAACA GAGAAGCTAT GTTTAGAAAA AGACAATGAA 
AATAAGCAGA AGGTTATTGT CTGCCTTGAA GAAGAACTCT CAGTGGTCAC AAGTGAGAGA 
AACCAGCTTC GTGGAGAATT AGATACTATG TCAAAAAAAA CCACGGCACT GGATCAGTTG 
TCTGAAAAAA TGAAGGAGAA AACACAAGAG CTTGAGTCTC ATCAAAGTGA GTGTCTCCAT 
TGCATTCAGG TGGCAGAGGC AGAGGTGAAG GAAAAGACGG AACTCCTTCA GACTTTGTCC 
TCTGATGTGA GTGAGCTGTT AAAAGACAAA ACTCATCTCC AGGAAAAGCT GCAGAGTTTG 
GAAAAGGACT CACAGGCACT GTCTTTGACA AAATGTGAGC TGGAAAACCA AATTGCACAA 
CTGAATAAAG AGAAAGAATT GCTTGTCAAG GAATCTGAAA GCCTGCAGGC CAGACTGAGT 
GAATCAGATT ATGAAAAGCT GAATGTCTCC AAGGCCTTGG AGGCCGCACT GGTGGAGAAA 
GGTGAGTTCG CATTGAGGCT GAGCTCAACA CAGGAGGAAG TGCATCAGCT GAGAAGAGGC 
ATCGAGAAAC TGAGAGTTCG CATTGAGGCC GATGAAAAGA AGCAGCTGCA CATCGCAGAG 
AAACTGAAAG AACGCGAGCG GGAGAATGAT TCACTTAAGG ATAAAGTTGA GAACCTTGAA 
AGGGAATTGC AGATGTCAGA AGAAAACCAG GAGCTAGTGA TTCTTGATGC CGAGAATTCC 
AAAGCAGAAG TAGAGACTCT AAAAACACAA ATAGAAGAGA TGGCCAGAAG CCTGAAAGTT 
TTTGAATTAG ACCTTGTCAC GTTAAGGTCT GAAAAAGAAA ATCTGACAAA ACAAATACAA 
GAAAAACAAG GTCAGTTGTC AGAACTAGAC AAGTTACTCT CTTCATTTAA AAGTCTGTTA 
GAAGAAAAGG AGCAAGCAGA GATACAGATC AAAGAAGAAT CTAAAACTGC AGTGGAGATG 
CTTCAGAATC AGTTAAAGGA GCTAAATGAG GCAGTAGCAG CCTTGTGTGG TGACCAAGAA 
ATTATGAAGG CCACAGAACA GAGTCTAGAC CCACCAATAG AGGAAGAGCA TCAGCTGAGA 
AATAGCATTG AAAAGCTGAG AGCCCGCCTA GAAGCTGATG AAAAGAAGCA GCTCTGTGTC 
TTACAACAAC TGAAGGAAAG TGAGCATCAT GCAGATTTAC TTAAGGGTAG AGTGGAGAAC 
CTTGAAAGAG AGCTAGAGAT AGCCAGGACA AACCAAGAGC ATGCAGCTCT TGAGGCAGAG 
AATTCCAAAG GAGAGGTAGA GACCCTAAAA GCAAAAATAG AAGGGATGAC CCAAAGTCTG 
AGAGGTCTGG AATTAGATGT TGTTACTATA AGGTCAGAAA AAGAAAATCT GACAAATGAA 
TTACAAAAAG AGCAAGAGCG AATATCTGAA TTAGAAATAA TAAATTCATC ATTTGAAAAT 
ATTTTGCAAG AAAAAGAGCA AGAGAAAGTA CAGATGAAAG AAAAATCAAG CACTGCCATG 
GAGATGCTTC AAACACAATT AAAAGAGCTC AATGAGAGAG TGGCAGCCCT GCATAATGAC 
CAAGAAGCCT GTAAGGCCAA AGAGCAGAAT CTTAGTAGTC AAGTAGAGTG TCTTGAACTT 
GAGAAGGCTC AGTTGCTACA AGGCCTTGAT GAGGCCAAAA ATAATTATAT TGTTTTGCAA 
TCTTCAGTGA ATGGCCTCAT TCAAGAAGTA GAAGATGGCA AGCAGAAACT GGAGAAGAAG 
GATGAAGAAA TCAGTAGACT GAAAAATCAA ATTCAAGACC AAGAGCAGCT TGTCTCTAAA 
CTGTCCCAGG TGGAAGGAGA GCACCAACTT TGGAAGGAGC AAAACTTAGA ACTGAGAAAT 
CTGACAGTGG AATTGGAGCA GAAGATCCAA GTGCTACAAT CCAAAAATGC CTCTTTGCAG 
GACACATTAG AAGTGCTGCA GAGTTCTTAC AAGAATCTAG AGAATGAGCT TGAATTGACA 
AAAATGGACA AAATGTCCTT TGTTGAAAAA GTAAACAAAA TGACTGCAAA GGAAACTGAG 
CTGCAGAGGG AAATGCATGA GATGGCACAG AAAACAGCAG AGCTGCAAGA AGAACTCAGT 
GGAGAGAAAA ATAGGCTAGC TGGAGAGTTG CAGTTACTGT TGGAAGAAAT AAAGAGCAGC 
AAAGATCAAT TGAAGGAGCT CACACTAGAA AATAGTGAAT TGAAGAAGAG CCTAGATTGC 
ATGCACAAAG ACCAGGTGGA AAAGGAAGGG AAAGTGAGAG AGGAAATAGC TGAATATCAG 
CTACGGCTTC ATGAAGCTGA AAAGAAACAC CAGGCTTTGC TTTTGGACAC AAACAAACAG 
TATGAAGTAG AAATCCAGAC ATACCGAGAG AAATTGACTT CTAAAGAAGA ATGTCTCAGT 
TCACAGAAGC TGGAGATAGA CCTTTTAAAG TCTAGTAAAG AAGAGCTCAA TAATTCATTG 
AAAGCTACTA CTCAGATTTT GGAAGAATTG AAGAAAACCA AGATGGACAA TCTAAAATAT 
GTAAATCAGT TGAAGAAGGA AAATGAACGT GCCCAGGGGA AAATGAAGTT GTTGATCAAA 
TCCTGTAAAC AGCTGGAAGA GGAAAAGGAG ATACTGCAGA AAGAACTCTC TCAACTTCAA 
GCTGCACAGG AGAAGCAGAA AACAGGTACT GTTATGGATA CCAAGGTCGA TGAATTAACA 
ACTGAGATCA AAGAACTGAA AGAAACTCTT GAAGAAAAAA CCAAGGAGGC AGATGAATAC 
TTGGATAAGT ACTGTTCCTT GCTTATAAGC CATGAAAAGT TAGAGAAAGC TAAAGAGATG 
TTAGAGACAC AAGTGGCCCA TCTGTGTTCA CAGCAATCTA AACAAGATTC CCGAGGGTCT 
CCTTTGCTAG GTCCAGTTGT TCCAGGACCA TCTCCAATCC CTTCTGTTAC TGAAAAGAGG 
TTATCATCTG GCCAAAATAA AGCTTCAGGC AAGAGGCAAA GATCCAGTGG AATATGGGAG 
AATGGTAGAG GACCAACACC TGCTACCCCA GAGAGCTTTT CTAAAAAAAG CAAGAAAGCA 
GTCATGAGTG GTATTCACCC TGCAGAAGAC ACGGAAGGTA CTGAGTTTGA GCCAGAGGGA 
CTTCCAGAAG TTGTAAAGAA AGGGTTTGCT GACATCCCGA CAGGAAAGAC TAGCCCATAT 
ATCCTGCGAA GAACAACCAT GGCAACTCGG ACCAGCCCCC GCCTGGCTGC ACAGAAGTTA 
GCGCTATCCC CACTGAGTCT CGGCAAAGAA AATCTTGCAG AGTCCTCCAA ACCAACAGCT 
GGTGGCAGCA GATCACAAAA GGTCAAAGTT GCTCAGCGGA GCCCAGTAGA TTCAGGCACC 
ATCCTCCGAG AACCCACCAC GAAATCCGTC CCAGTCAATA ATCTTCCTGA GAGAAGTCCG 
ACTGACAGCC CCAGAGAGGG CCTGAGGGTC AAGCGAGGCC GACTTGTCCC CAGCCCCAAA 
GCTGGACTGG AGTCCAACGG CAGTGAGAAC TGTAAGGTCC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CENPF ( NM_016343.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_057427.3 Homo sapiens centromere protein F (CENPF), mRNA.

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CloneID	OHu14151
Clone ID Related Accession (Same CDS sequence)	XM_017000086.1 , NM_016343.4 , NM_016343.3 , XM_017000086.2
Accession Version	NM_016343.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9345bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-05
Organism	Homo sapiens(human)
Product	centromere protein F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BU662228.1, U30872.1, BU155312.1, CD654009.1, BG680819.1 and BQ774943.1. This sequence is a reference standard in the RefSeqGene project. On or before Nov 15, 2004 this sequence version replaced NM_005196.1, NM_016343.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U30872.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGCTGGG CTTTGGAAGA ATGGAAAGAA GGGCTGCCTA CAAGAGCTCT TCAGAAAATT 
CAAGAGCTTG AAGGACAGCT TGACAAACTG AAGAAGGAAA AGCAGCAAAG GCAGTTTCAG 
CTTGACAGTC TCGAGGCTGC GCTGCAGAAG CAAAAACAGA AGGTTGAAAA TGAAAAAACC 
GAGGGTACAA ACCTGAAAAG GGAGAATCAA AGATTGATGG AAATATGTGA AAGTCTGGAG 
AAAACTAAGC AGAAGATTTC TCATGAACTT CAAGTCAAGG AGTCACAAGT GAATTTCCAG 
GAAGGACAAC TGAATTCAGG CAAAAAACAA ATAGAAAAAC TGGAACAGGA ACTTAAAAGG 
TGTAAATCTG AGCTTGAAAG AAGCCAACAA GCTGCGCAGT CTGCAGATGT CTCTCTGAAT 
CCATGCAATA CACCACAAAA AATTTTTACA ACTCCACTAA CACCAAGTCA ATATTATAGT 
GGTTCCAAGT ATGAAGATCT AAAAGAAAAA TATAATAAAG AGGTTGAAGA ACGAAAAAGA 
TTAGAGGCAG AGGTTAAAGC CTTGCAGGCT AAAAAAGCAA GCCAGACTCT TCCACAAGCC 
ACCATGAATC ACCGCGACAT TGCCCGGCAT CAGGCTTCAT CATCTGTGTT CTCATGGCAG 
CAAGAGAAGA CCCCAAGTCA TCTTTCATCT AATTCTCAAA GAACTCCAAT TAGGAGAGAT 
TTCTCTGCAT CTTACTTTTC TGGGGAACAA GAGGTGACTC CAAGTCGATC AACTTTGCAA 
ATAGGGAAAA GAGATGCTAA TAGCAGTTTC TTTGACAATT CTAGCAGTCC TCATCTTTTG 
GATCAATTAA AAGCGCAGAA TCAAGAGCTA AGAAACAAGA TTAATGAGTT GGAACTACGC 
CTGCAAGGAC ATGAAAAAGA AATGAAAGGC CAAGTGAATA AGTTTCAAGA ACTCCAACTC 
CAACTGGAGA AAGCAAAAGT GGAATTAATT GAAAAAGAGA AAGTTTTGAA CAAATGTAGG 
GATGAACTAG TGAGAACAAC AGCACAATAC GACCAGGCGT CAACCAAGTA TACTGCATTG 
GAACAAAAAC TGAAAAAATT GACGGAAGAT TTGAGTTGTC AGCGACAAAA TGCAGAAAGT 
GCCAGATGTT CTCTGGAACA GAAAATTAAG GAAAAAGAAA AGGAGTTTCA AGAGGAGCTC 
TCCCGTCAAC AGCGTTCTTT CCAAACACTG GACCAGGAGT GCATCCAGAT GAAGGCCAGA 
CTCACCCAGG AGTTACAGCA AGCCAAGAAT ATGCACAACG TCCTGCAGGC TGAACTGGAT 
AAACTCACAT CAGTAAAGCA ACAGCTAGAA AACAATTTGG AAGAGTTTAA GCAAAAGTTG 
TGCAGAGCTG AACAGGCGTT CCAGGCGAGT CAGATCAAGG AGAATGAGCT GAGGAGAAGC 
ATGGAGGAAA TGAAGAAGGA AAACAACCTC CTTAAGAGTC ACTCTGAGCA AAAGGCCAGA 
GAAGTCTGCC ACCTGGAGGC AGAACTCAAG AACATCAAAC AGTGTTTAAA TCAGAGCCAG 
AATTTTGCAG AAGAAATGAA AGCGAAGAAT ACCTCTCAGG AAACCATGTT AAGAGATCTT 
CAAGAAAAAA TAAATCAGCA AGAAAACTCC TTGACTTTAG AAAAACTGAA GCTTGCTGTG 
GCTGATCTGG AAAAGCAGCG AGATTGTTCT CAAGACCTTT TGAAGAAAAG AGAACATCAC 
ATTGAACAAC TTAATGATAA GTTAAGCAAG ACAGAGAAAG AGTCCAAAGC CTTGCTGAGT 
GCTTTAGAGT TAAAAAAGAA AGAATATGAA GAATTGAAAG AAGAGAAAAC TCTGTTTTCT 
TGTTGGAAAA GTGAAAACGA AAAACTTTTA ACTCAGATGG AATCAGAAAA GGAAAACTTG 
CAGAGTAAAA TTAATCACTT GGAAACTTGT CTGAAGACAC AGCAAATAAA AAGTCATGAA 
TACAACGAGA GAGTAAGAAC GCTGGAGATG GACAGAGAAA ACCTAAGTGT CGAGATCAGA 
AACCTTCACA ACGTGTTAGA CAGTAAGTCA GTGGAGGTAG AGACCCAGAA ACTAGCTTAT 
ATGGAGCTAC AGCAGAAAGC TGAGTTCTCA GATCAGAAAC ATCAGAAGGA AATAGAAAAT 
ATGTGTTTGA AGACTTCTCA GCTTACTGGG CAAGTTGAAG ATCTAGAACA CAAGCTTCAG 
TTACTGTCAA ATGAAATAAT GGACAAAGAC CGGTGTTACC AAGACTTGCA TGCCGAATAT 
GAGAGCCTCA GGGATCTGCT AAAATCCAAA GATGCTTCTC TGGTGACAAA TGAAGATCAT 
CAGAGAAGTC TTTTGGCTTT TGATCAGCAG CCTGCCATGC ATCATTCCTT TGCAAATATA 
ATTGGAGAAC AAGGAAGCAT GCCTTCAGAG AGGAGTGAAT GTCGTTTAGA AGCAGACCAA 
AGTCCGAAAA ATTCTGCCAT CCTACAAAAT AGAGTTGATT CACTTGAATT TTCATTAGAG 
TCTCAAAAAC AGATGAACTC AGACCTGCAA AAGCAGTGTG AAGAGTTGGT GCAAATCAAA 
GGAGAAATAG AAGAAAATCT CATGAAAGCA GAACAGATGC ATCAAAGTTT TGTGGCTGAA 
ACAAGTCAGC GCATTAGTAA GTTACAGGAA GACACTTCTG CTCACCAGAA TGTTGTTGCT 
GAAACCTTAA GTGCCCTTGA GAACAAGGAA AAAGAGCTGC AACTTTTAAA TGATAAGGTA 
GAAACTGAGC AGGCAGAGAT TCAAGAATTA AAAAAGAGCA ACCATCTACT TGAAGACTCT 
CTAAAGGAGC TACAACTTTT ATCCGAAACC CTAAGCTTGG AGAAGAAAGA AATGAGTTCC 
ATCATTTCTC TAAATAAAAG GGAAATTGAA GAGCTGACCC AAGAGAATGG GACTCTTAAG 
GAAATTAATG CATCCTTAAA TCAAGAGAAG ATGAACTTAA TCCAGAAAAG TGAGAGTTTT 
GCAAACTATA TAGATGAAAG GGAGAAAAGC ATTTCAGAGT TATCTGATCA GTACAAGCAA 
GAAAAACTTA TTTTACTACA AAGATGTGAA GAAACCGGAA ATGCATATGA GGATCTTAGT 
CAAAAATACA AAGCAGCACA GGAAAAGAAT TCTAAATTAG AATGCTTGCT AAATGAATGC 
ACTAGTCTTT GTGAAAATAG GAAAAATGAG TTGGAACAGC TAAAGGAAGC ATTTGCAAAG 
GAACACCAAG AATTCTTAAC AAAATTAGCA TTTGCTGAAG AAAGAAATCA GAATCTGATG 
CTAGAGTTGG AGACAGTGCA GCAAGCTCTG AGATCTGAGA TGACAGATAA CCAAAACAAT 
TCTAAGAGCG AGGCTGGTGG TTTAAAGCAA GAAATCATGA CTTTAAAGGA AGAACAAAAC 
AAAATGCAAA AGGAAGTTAA TGACTTATTA CAAGAGAATG AACAGCTGAT GAAGGTAATG 
AAGACTAAAC ATGAATGTCA AAATCTAGAA TCAGAACCAA TTAGGAACTC TGTGAAAGAA 
AGAGAGAGTG AGAGAAATCA ATGTAATTTT AAACCTCAGA TGGATCTTGA AGTTAAAGAA 
ATTTCTCTAG ATAGTTATAA TGCGCAGTTG GTGCAATTAG AAGCTATGCT AAGAAATAAG 
GAATTAAAAC TTCAGGAAAG TGAGAAGGAG AAGGAGTGCC TGCAGCATGA ATTACAGACA 
ATTAGAGGAG ATCTTGAAAC CAGCAATTTG CAAGACATGC AGTCACAAGA AATTAGTGGC 
CTTAAAGACT GTGAAATAGA TGCGGAAGAA AAGTATATTT CAGGGCCTCA TGAGTTGTCA 
ACAAGTCAAA ACGACAATGC ACACCTTCAG TGCTCTCTGC AAACAACAAT GAACAAGCTG 
AATGAGCTAG AGAAAATATG TGAAATACTG CAGGCTGAAA AGTATGAACT CGTAACTGAG 
CTGAATGATT CAAGGTCAGA ATGTATCACA GCAACTAGGA AAATGGCAGA AGAGGTAGGG 
AAACTACTAA ATGAAGTTAA AATATTAAAT GATGACAGTG GTCTTCTCCA TGGTGAGTTA 
GTGGAAGACA TACCAGGAGG TGAATTTGGT GAACAACCAA ATGAACAGCA CCCTGTGTCT 
TTGGCTCCAT TGGACGAGAG TAATTCCTAC GAGCACTTGA CATTGTCAGA CAAAGAAGTT 
CAAATGCACT TTGCCGAATT GCAAGAGAAA TTCTTATCTT TACAAAGTGA ACACAAAATT 
TTACATGATC AGCACTGTCA GATGAGCTCT AAAATGTCAG AGCTGCAGAC CTATGTTGAC 
TCATTAAAGG CCGAAAATTT GGTCTTGTCA ACGAATCTGA GAAACTTTCA AGGTGACTTG 
GTGAAGGAGA TGCAGCTGGG CTTGGAGGAG GGGCTCGTTC CATCCCTGTC ATCCTCTTGT 
GTGCCTGACA GCTCTAGTCT TAGCAGTTTG GGAGACTCCT CCTTTTACAG AGCTCTTTTA 
GAACAGACAG GAGATATGTC TCTTTTGAGT AATTTAGAAG GGGCTGTTTC AGCAAACCAG 
TGCAGTGTAG ATGAAGTATT TTGCAGCAGT CTGCAGGAGG AGAATCTGAC CAGGAAAGAA 
ACCCCTTCGG CCCCAGCGAA GGGTGTTGAA GAGCTTGAGT CCCTCTGTGA GGTGTACCGG 
CAGTCCCTCG AGAAGCTAGA AGAGAAAATG GAAAGTCAAG GGATTATGAA AAATAAGGAA 
ATTCAAGAGC TCGAGCAGTT ATTAAGTTCT GAAAGGCAAG AGCTTGACTG CCTTAGGAAG 
CAGTATTTGT CAGAAAATGA ACAGTGGCAA CAGAAGCTGA CAAGCGTGAC TCTGGAGATG 
GAGTCCAAGT TGGCGGCAGA AAAGAAACAG ACGGAACAAC TGTCACTTGA GCTGGAAGTA 
GCACGACTCC AGCTACAAGG TCTGGACTTA AGTTCTCGGT CTTTGCTTGG CATCGACACA 
GAAGATGCTA TTCAAGGCCG AAATGAGAGC TGTGACATAT CAAAAGAACA TACTTCAGAA 
ACTACAGAAA GAACACCAAA GCATGATGTT CATCAGATTT GTGATAAAGA TGCTCAGCAG 
GACCTCAATC TAGACATTGA GAAAATAACT GAGACTGGTG CAGTGAAACC CACAGGAGAG 
TGCTCTGGGG AACAGTCCCC AGATACCAAT TATGAGCCTC CAGGGGAAGA TAAAACCCAG 
GGCTCTTCAG AATGCATTTC TGAATTGTCA TTTTCTGGTC CTAATGCTTT GGTACCTATG 
GATTTCCTGG GGAATCAGGA AGATATCCAT AATCTTCAAC TGCGGGTAAA AGAGACATCA 
AATGAGAATT TGAGATTACT TCATGTGATA GAGGACCGTG ACAGAAAAGT TGAAAGTTTG 
CTAAATGAAA TGAAAGAATT AGACTCAAAA CTCCATTTAC AGGAGGTACA ACTAATGACC 
AAAATTGAAG CATGCATAGA ATTGGAAAAA ATAGTTGGGG AACTTAAGAA AGAAAACTCA 
GATTTAAGTG AAAAATTGGA ATATTTTTCT TGTGATCACC AGGAGTTACT CCAGAGAGTA 
GAAACTTCTG AAGGCCTCAA TTCTGATTTA GAAATGCATG CAGATAAATC ATCACGTGAA 
GATATTGGAG ATAATGTGGC CAAGGTGAAT GACAGCTGGA AGGAGAGATT TCTTGATGTG 
GAAAATGAGC TGAGTAGGAT CAGATCGGAG AAAGCTAGCA TTGAGCATGA AGCCCTCTAC 
CTGGAGGCTG ACTTAGAGGT AGTTCAAACA GAGAAGCTAT GTTTAGAAAA AGACAATGAA 
AATAAGCAGA AGGTTATTGT CTGCCTTGAA GAAGAACTCT CAGTGGTCAC AAGTGAGAGA 
AACCAGCTTC GTGGAGAATT AGATACTATG TCAAAAAAAA CCACGGCACT GGATCAGTTG 
TCTGAAAAAA TGAAGGAGAA AACACAAGAG CTTGAGTCTC ATCAAAGTGA GTGTCTCCAT 
TGCATTCAGG TGGCAGAGGC AGAGGTGAAG GAAAAGACGG AACTCCTTCA GACTTTGTCC 
TCTGATGTGA GTGAGCTGTT AAAAGACAAA ACTCATCTCC AGGAAAAGCT GCAGAGTTTG 
GAAAAGGACT CACAGGCACT GTCTTTGACA AAATGTGAGC TGGAAAACCA AATTGCACAA 
CTGAATAAAG AGAAAGAATT GCTTGTCAAG GAATCTGAAA GCCTGCAGGC CAGACTGAGT 
GAATCAGATT ATGAAAAGCT GAATGTCTCC AAGGCCTTGG AGGCCGCACT GGTGGAGAAA 
GGTGAGTTCG CATTGAGGCT GAGCTCAACA CAGGAGGAAG TGCATCAGCT GAGAAGAGGC 
ATCGAGAAAC TGAGAGTTCG CATTGAGGCC GATGAAAAGA AGCAGCTGCA CATCGCAGAG 
AAACTGAAAG AACGCGAGCG GGAGAATGAT TCACTTAAGG ATAAAGTTGA GAACCTTGAA 
AGGGAATTGC AGATGTCAGA AGAAAACCAG GAGCTAGTGA TTCTTGATGC CGAGAATTCC 
AAAGCAGAAG TAGAGACTCT AAAAACACAA ATAGAAGAGA TGGCCAGAAG CCTGAAAGTT 
TTTGAATTAG ACCTTGTCAC GTTAAGGTCT GAAAAAGAAA ATCTGACAAA ACAAATACAA 
GAAAAACAAG GTCAGTTGTC AGAACTAGAC AAGTTACTCT CTTCATTTAA AAGTCTGTTA 
GAAGAAAAGG AGCAAGCAGA GATACAGATC AAAGAAGAAT CTAAAACTGC AGTGGAGATG 
CTTCAGAATC AGTTAAAGGA GCTAAATGAG GCAGTAGCAG CCTTGTGTGG TGACCAAGAA 
ATTATGAAGG CCACAGAACA GAGTCTAGAC CCACCAATAG AGGAAGAGCA TCAGCTGAGA 
AATAGCATTG AAAAGCTGAG AGCCCGCCTA GAAGCTGATG AAAAGAAGCA GCTCTGTGTC 
TTACAACAAC TGAAGGAAAG TGAGCATCAT GCAGATTTAC TTAAGGGTAG AGTGGAGAAC 
CTTGAAAGAG AGCTAGAGAT AGCCAGGACA AACCAAGAGC ATGCAGCTCT TGAGGCAGAG 
AATTCCAAAG GAGAGGTAGA GACCCTAAAA GCAAAAATAG AAGGGATGAC CCAAAGTCTG 
AGAGGTCTGG AATTAGATGT TGTTACTATA AGGTCAGAAA AAGAAAATCT GACAAATGAA 
TTACAAAAAG AGCAAGAGCG AATATCTGAA TTAGAAATAA TAAATTCATC ATTTGAAAAT 
ATTTTGCAAG AAAAAGAGCA AGAGAAAGTA CAGATGAAAG AAAAATCAAG CACTGCCATG 
GAGATGCTTC AAACACAATT AAAAGAGCTC AATGAGAGAG TGGCAGCCCT GCATAATGAC 
CAAGAAGCCT GTAAGGCCAA AGAGCAGAAT CTTAGTAGTC AAGTAGAGTG TCTTGAACTT 
GAGAAGGCTC AGTTGCTACA AGGCCTTGAT GAGGCCAAAA ATAATTATAT TGTTTTGCAA 
TCTTCAGTGA ATGGCCTCAT TCAAGAAGTA GAAGATGGCA AGCAGAAACT GGAGAAGAAG 
GATGAAGAAA TCAGTAGACT GAAAAATCAA ATTCAAGACC AAGAGCAGCT TGTCTCTAAA 
CTGTCCCAGG TGGAAGGAGA GCACCAACTT TGGAAGGAGC AAAACTTAGA ACTGAGAAAT 
CTGACAGTGG AATTGGAGCA GAAGATCCAA GTGCTACAAT CCAAAAATGC CTCTTTGCAG 
GACACATTAG AAGTGCTGCA GAGTTCTTAC AAGAATCTAG AGAATGAGCT TGAATTGACA 
AAAATGGACA AAATGTCCTT TGTTGAAAAA GTAAACAAAA TGACTGCAAA GGAAACTGAG 
CTGCAGAGGG AAATGCATGA GATGGCACAG AAAACAGCAG AGCTGCAAGA AGAACTCAGT 
GGAGAGAAAA ATAGGCTAGC TGGAGAGTTG CAGTTACTGT TGGAAGAAAT AAAGAGCAGC 
AAAGATCAAT TGAAGGAGCT CACACTAGAA AATAGTGAAT TGAAGAAGAG CCTAGATTGC 
ATGCACAAAG ACCAGGTGGA AAAGGAAGGG AAAGTGAGAG AGGAAATAGC TGAATATCAG 
CTACGGCTTC ATGAAGCTGA AAAGAAACAC CAGGCTTTGC TTTTGGACAC AAACAAACAG 
TATGAAGTAG AAATCCAGAC ATACCGAGAG AAATTGACTT CTAAAGAAGA ATGTCTCAGT 
TCACAGAAGC TGGAGATAGA CCTTTTAAAG TCTAGTAAAG AAGAGCTCAA TAATTCATTG 
AAAGCTACTA CTCAGATTTT GGAAGAATTG AAGAAAACCA AGATGGACAA TCTAAAATAT 
GTAAATCAGT TGAAGAAGGA AAATGAACGT GCCCAGGGGA AAATGAAGTT GTTGATCAAA 
TCCTGTAAAC AGCTGGAAGA GGAAAAGGAG ATACTGCAGA AAGAACTCTC TCAACTTCAA 
GCTGCACAGG AGAAGCAGAA AACAGGTACT GTTATGGATA CCAAGGTCGA TGAATTAACA 
ACTGAGATCA AAGAACTGAA AGAAACTCTT GAAGAAAAAA CCAAGGAGGC AGATGAATAC 
TTGGATAAGT ACTGTTCCTT GCTTATAAGC CATGAAAAGT TAGAGAAAGC TAAAGAGATG 
TTAGAGACAC AAGTGGCCCA TCTGTGTTCA CAGCAATCTA AACAAGATTC CCGAGGGTCT 
CCTTTGCTAG GTCCAGTTGT TCCAGGACCA TCTCCAATCC CTTCTGTTAC TGAAAAGAGG 
TTATCATCTG GCCAAAATAA AGCTTCAGGC AAGAGGCAAA GATCCAGTGG AATATGGGAG 
AATGGTAGAG GACCAACACC TGCTACCCCA GAGAGCTTTT CTAAAAAAAG CAAGAAAGCA 
GTCATGAGTG GTATTCACCC TGCAGAAGAC ACGGAAGGTA CTGAGTTTGA GCCAGAGGGA 
CTTCCAGAAG TTGTAAAGAA AGGGTTTGCT GACATCCCGA CAGGAAAGAC TAGCCCATAT 
ATCCTGCGAA GAACAACCAT GGCAACTCGG ACCAGCCCCC GCCTGGCTGC ACAGAAGTTA 
GCGCTATCCC CACTGAGTCT CGGCAAAGAA AATCTTGCAG AGTCCTCCAA ACCAACAGCT 
GGTGGCAGCA GATCACAAAA GGTCAAAGTT GCTCAGCGGA GCCCAGTAGA TTCAGGCACC 
ATCCTCCGAG AACCCACCAC GAAATCCGTC CCAGTCAATA ATCTTCCTGA GAGAAGTCCG 
ACTGACAGCC CCAGAGAGGG CCTGAGGGTC AAGCGAGGCC GACTTGTCCC CAGCCCCAAA 
GCTGGACTGG AGTCCAACGG CAGTGAGAAC TGTAAGGTCC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_057427.3
CDS	175..9519
Translation	MSWALEEWKEGLPTRALQKIQELEGQLDKLKKEKQQRQFQLDSLEAALQKQKQKVENEKTEGTNLKRENQRLMEICESLEKTKQKISHELQVKESQVNFQEGQLNSGKKQIEKLEQELKRCKSELERSQQAAQSADVSLNPCNTPQKIFTTPLTPSQYYSGSKYEDLKEKYNKEVEERKRLEAEVKALQAKKASQTLPQATMNHRDIARHQASSSVFSWQQEKTPSHLSSNSQRTPIRRDFSASYFSGEQEVTPSRSTLQIGKRDANSSFFDNSSSPHLLDQLKAQNQELRNKINELELRLQGHEKEMKGQVNKFQELQLQLEKAKVELIEKEKVLNKCRDELVRTTAQYDQASTKYTALEQKLKKLTEDLSCQRQNAESARCSLEQKIKEKEKEFQEELSRQQRSFQTLDQECIQMKARLTQELQQAKNMHNVLQAELDKLTSVKQQLENNLEEFKQKLCRAEQAFQASQIKENELRRSMEEMKKENNLLKSHSEQKAREVCHLEAELKNIKQCLNQSQNFAEEMKAKNTSQETMLRDLQEKINQQENSLTLEKLKLAVADLEKQRDCSQDLLKKREHHIEQLNDKLSKTEKESKALLSALELKKKEYEELKEEKTLFSCWKSENEKLLTQMESEKENLQSKINHLETCLKTQQIKSHEYNERVRTLEMDRENLSVEIRNLHNVLDSKSVEVETQKLAYMELQQKAEFSDQKHQKEIENMCLKTSQLTGQVEDLEHKLQLLSNEIMDKDRCYQDLHAEYESLRDLLKSKDASLVTNEDHQRSLLAFDQQPAMHHSFANIIGEQGSMPSERSECRLEADQSPKNSAILQNRVDSLEFSLESQKQMNSDLQKQCEELVQIKGEIEENLMKAEQMHQSFVAETSQRISKLQEDTSAHQNVVAETLSALENKEKELQLLNDKVETEQAEIQELKKSNHLLEDSLKELQLLSETLSLEKKEMSSIISLNKREIEELTQENGTLKEINASLNQEKMNLIQKSESFANYIDEREKSISELSDQYKQEKLILLQRCEETGNAYEDLSQKYKAAQEKNSKLECLLNECTSLCENRKNELEQLKEAFAKEHQEFLTKLAFAEERNQNLMLELETVQQALRSEMTDNQNNSKSEAGGLKQEIMTLKEEQNKMQKEVNDLLQENEQLMKVMKTKHECQNLESEPIRNSVKERESERNQCNFKPQMDLEVKEISLDSYNAQLVQLEAMLRNKELKLQESEKEKECLQHELQTIRGDLETSNLQDMQSQEISGLKDCEIDAEEKYISGPHELSTSQNDNAHLQCSLQTTMNKLNELEKICEILQAEKYELVTELNDSRSECITATRKMAEEVGKLLNEVKILNDDSGLLHGELVEDIPGGEFGEQPNEQHPVSLAPLDESNSYEHLTLSDKEVQMHFAELQEKFLSLQSEHKILHDQHCQMSSKMSELQTYVDSLKAENLVLSTNLRNFQGDLVKEMQLGLEEGLVPSLSSSCVPDSSSLSSLGDSSFYRALLEQTGDMSLLSNLEGAVSANQCSVDEVFCSSLQEENLTRKETPSAPAKGVEELESLCEVYRQSLEKLEEKMESQGIMKNKEIQELEQLLSSERQELDCLRKQYLSENEQWQQKLTSVTLEMESKLAAEKKQTEQLSLELEVARLQLQGLDLSSRSLLGIDTEDAIQGRNESCDISKEHTSETTERTPKHDVHQICDKDAQQDLNLDIEKITETGAVKPTGECSGEQSPDTNYEPPGEDKTQGSSECISELSFSGPNALVPMDFLGNQEDIHNLQLRVKETSNENLRLLHVIEDRDRKVESLLNEMKELDSKLHLQEVQLMTKIEACIELEKIVGELKKENSDLSEKLEYFSCDHQELLQRVETSEGLNSDLEMHADKSSREDIGDNVAKVNDSWKERFLDVENELSRIRSEKASIEHEALYLEADLEVVQTEKLCLEKDNENKQKVIVCLEEELSVVTSERNQLRGELDTMSKKTTALDQLSEKMKEKTQELESHQSECLHCIQVAEAEVKEKTELLQTLSSDVSELLKDKTHLQEKLQSLEKDSQALSLTKCELENQIAQLNKEKELLVKESESLQARLSESDYEKLNVSKALEAALVEKGEFALRLSSTQEEVHQLRRGIEKLRVRIEADEKKQLHIAEKLKERERENDSLKDKVENLERELQMSEENQELVILDAENSKAEVETLKTQIEEMARSLKVFELDLVTLRSEKENLTKQIQEKQGQLSELDKLLSSFKSLLEEKEQAEIQIKEESKTAVEMLQNQLKELNEAVAALCGDQEIMKATEQSLDPPIEEEHQLRNSIEKLRARLEADEKKQLCVLQQLKESEHHADLLKGRVENLERELEIARTNQEHAALEAENSKGEVETLKAKIEGMTQSLRGLELDVVTIRSEKENLTNELQKEQERISELEIINSSFENILQEKEQEKVQMKEKSSTAMEMLQTQLKELNERVAALHNDQEACKAKEQNLSSQVECLELEKAQLLQGLDEAKNNYIVLQSSVNGLIQEVEDGKQKLEKKDEEISRLKNQIQDQEQLVSKLSQVEGEHQLWKEQNLELRNLTVELEQKIQVLQSKNASLQDTLEVLQSSYKNLENELELTKMDKMSFVEKVNKMTAKETELQREMHEMAQKTAELQEELSGEKNRLAGELQLLLEEIKSSKDQLKELTLENSELKKSLDCMHKDQVEKEGKVREEIAEYQLRLHEAEKKHQALLLDTNKQYEVEIQTYREKLTSKEECLSSQKLEIDLLKSSKEELNNSLKATTQILEELKKTKMDNLKYVNQLKKENERAQGKMKLLIKSCKQLEEEKEILQKELSQLQAAQEKQKTGTVMDTKVDELTTEIKELKETLEEKTKEADEYLDKYCSLLISHEKLEKAKEMLETQVAHLCSQQSKQDSRGSPLLGPVVPGPSPIPSVTEKRLSSGQNKASGKRQRSSGIWENGRGPTPATPESFSKKSKKAVMSGIHPAEDTEGTEFEPEGLPEVVKKGFADIPTGKTSPYILRRTTMATRTSPRLAAQKLALSPLSLGKENLAESSKPTAGGSRSQKVKVAQRSPVDSGTILREPTTKSVPVNNLPERSPTDSPREGLRVKRGRLVPSPKAGLESNGSENCKVQ

Target ORF information:

RefSeq Version	NM_016343.3
Organism	Homo sapiens(human)
Definition	Homo sapiens centromere protein F (CENPF), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_016343.3

ORF Insert Sequence:

ATGAGCTGGG CTTTGGAAGA ATGGAAAGAA GGGCTGCCTA CAAGAGCTCT TCAGAAAATT 
CAAGAGCTTG AAGGACAGCT TGACAAACTG AAGAAGGAAA AGCAGCAAAG GCAGTTTCAG 
CTTGACAGTC TCGAGGCTGC GCTGCAGAAG CAAAAACAGA AGGTTGAAAA TGAAAAAACC 
GAGGGTACAA ACCTGAAAAG GGAGAATCAA AGATTGATGG AAATATGTGA AAGTCTGGAG 
AAAACTAAGC AGAAGATTTC TCATGAACTT CAAGTCAAGG AGTCACAAGT GAATTTCCAG 
GAAGGACAAC TGAATTCAGG CAAAAAACAA ATAGAAAAAC TGGAACAGGA ACTTAAAAGG 
TGTAAATCTG AGCTTGAAAG AAGCCAACAA GCTGCGCAGT CTGCAGATGT CTCTCTGAAT 
CCATGCAATA CACCACAAAA AATTTTTACA ACTCCACTAA CACCAAGTCA ATATTATAGT 
GGTTCCAAGT ATGAAGATCT AAAAGAAAAA TATAATAAAG AGGTTGAAGA ACGAAAAAGA 
TTAGAGGCAG AGGTTAAAGC CTTGCAGGCT AAAAAAGCAA GCCAGACTCT TCCACAAGCC 
ACCATGAATC ACCGCGACAT TGCCCGGCAT CAGGCTTCAT CATCTGTGTT CTCATGGCAG 
CAAGAGAAGA CCCCAAGTCA TCTTTCATCT AATTCTCAAA GAACTCCAAT TAGGAGAGAT 
TTCTCTGCAT CTTACTTTTC TGGGGAACAA GAGGTGACTC CAAGTCGATC AACTTTGCAA 
ATAGGGAAAA GAGATGCTAA TAGCAGTTTC TTTGACAATT CTAGCAGTCC TCATCTTTTG 
GATCAATTAA AAGCGCAGAA TCAAGAGCTA AGAAACAAGA TTAATGAGTT GGAACTACGC 
CTGCAAGGAC ATGAAAAAGA AATGAAAGGC CAAGTGAATA AGTTTCAAGA ACTCCAACTC 
CAACTGGAGA AAGCAAAAGT GGAATTAATT GAAAAAGAGA AAGTTTTGAA CAAATGTAGG 
GATGAACTAG TGAGAACAAC AGCACAATAC GACCAGGCGT CAACCAAGTA TACTGCATTG 
GAACAAAAAC TGAAAAAATT GACGGAAGAT TTGAGTTGTC AGCGACAAAA TGCAGAAAGT 
GCCAGATGTT CTCTGGAACA GAAAATTAAG GAAAAAGAAA AGGAGTTTCA AGAGGAGCTC 
TCCCGTCAAC AGCGTTCTTT CCAAACACTG GACCAGGAGT GCATCCAGAT GAAGGCCAGA 
CTCACCCAGG AGTTACAGCA AGCCAAGAAT ATGCACAACG TCCTGCAGGC TGAACTGGAT 
AAACTCACAT CAGTAAAGCA ACAGCTAGAA AACAATTTGG AAGAGTTTAA GCAAAAGTTG 
TGCAGAGCTG AACAGGCGTT CCAGGCGAGT CAGATCAAGG AGAATGAGCT GAGGAGAAGC 
ATGGAGGAAA TGAAGAAGGA AAACAACCTC CTTAAGAGTC ACTCTGAGCA AAAGGCCAGA 
GAAGTCTGCC ACCTGGAGGC AGAACTCAAG AACATCAAAC AGTGTTTAAA TCAGAGCCAG 
AATTTTGCAG AAGAAATGAA AGCGAAGAAT ACCTCTCAGG AAACCATGTT AAGAGATCTT 
CAAGAAAAAA TAAATCAGCA AGAAAACTCC TTGACTTTAG AAAAACTGAA GCTTGCTGTG 
GCTGATCTGG AAAAGCAGCG AGATTGTTCT CAAGACCTTT TGAAGAAAAG AGAACATCAC 
ATTGAACAAC TTAATGATAA GTTAAGCAAG ACAGAGAAAG AGTCCAAAGC CTTGCTGAGT 
GCTTTAGAGT TAAAAAAGAA AGAATATGAA GAATTGAAAG AAGAGAAAAC TCTGTTTTCT 
TGTTGGAAAA GTGAAAACGA AAAACTTTTA ACTCAGATGG AATCAGAAAA GGAAAACTTG 
CAGAGTAAAA TTAATCACTT GGAAACTTGT CTGAAGACAC AGCAAATAAA AAGTCATGAA 
TACAACGAGA GAGTAAGAAC GCTGGAGATG GACAGAGAAA ACCTAAGTGT CGAGATCAGA 
AACCTTCACA ACGTGTTAGA CAGTAAGTCA GTGGAGGTAG AGACCCAGAA ACTAGCTTAT 
ATGGAGCTAC AGCAGAAAGC TGAGTTCTCA GATCAGAAAC ATCAGAAGGA AATAGAAAAT 
ATGTGTTTGA AGACTTCTCA GCTTACTGGG CAAGTTGAAG ATCTAGAACA CAAGCTTCAG 
TTACTGTCAA ATGAAATAAT GGACAAAGAC CGGTGTTACC AAGACTTGCA TGCCGAATAT 
GAGAGCCTCA GGGATCTGCT AAAATCCAAA GATGCTTCTC TGGTGACAAA TGAAGATCAT 
CAGAGAAGTC TTTTGGCTTT TGATCAGCAG CCTGCCATGC ATCATTCCTT TGCAAATATA 
ATTGGAGAAC AAGGAAGCAT GCCTTCAGAG AGGAGTGAAT GTCGTTTAGA AGCAGACCAA 
AGTCCGAAAA ATTCTGCCAT CCTACAAAAT AGAGTTGATT CACTTGAATT TTCATTAGAG 
TCTCAAAAAC AGATGAACTC AGACCTGCAA AAGCAGTGTG AAGAGTTGGT GCAAATCAAA 
GGAGAAATAG AAGAAAATCT CATGAAAGCA GAACAGATGC ATCAAAGTTT TGTGGCTGAA 
ACAAGTCAGC GCATTAGTAA GTTACAGGAA GACACTTCTG CTCACCAGAA TGTTGTTGCT 
GAAACCTTAA GTGCCCTTGA GAACAAGGAA AAAGAGCTGC AACTTTTAAA TGATAAGGTA 
GAAACTGAGC AGGCAGAGAT TCAAGAATTA AAAAAGAGCA ACCATCTACT TGAAGACTCT 
CTAAAGGAGC TACAACTTTT ATCCGAAACC CTAAGCTTGG AGAAGAAAGA AATGAGTTCC 
ATCATTTCTC TAAATAAAAG GGAAATTGAA GAGCTGACCC AAGAGAATGG GACTCTTAAG 
GAAATTAATG CATCCTTAAA TCAAGAGAAG ATGAACTTAA TCCAGAAAAG TGAGAGTTTT 
GCAAACTATA TAGATGAAAG GGAGAAAAGC ATTTCAGAGT TATCTGATCA GTACAAGCAA 
GAAAAACTTA TTTTACTACA AAGATGTGAA GAAACCGGAA ATGCATATGA GGATCTTAGT 
CAAAAATACA AAGCAGCACA GGAAAAGAAT TCTAAATTAG AATGCTTGCT AAATGAATGC 
ACTAGTCTTT GTGAAAATAG GAAAAATGAG TTGGAACAGC TAAAGGAAGC ATTTGCAAAG 
GAACACCAAG AATTCTTAAC AAAATTAGCA TTTGCTGAAG AAAGAAATCA GAATCTGATG 
CTAGAGTTGG AGACAGTGCA GCAAGCTCTG AGATCTGAGA TGACAGATAA CCAAAACAAT 
TCTAAGAGCG AGGCTGGTGG TTTAAAGCAA GAAATCATGA CTTTAAAGGA AGAACAAAAC 
AAAATGCAAA AGGAAGTTAA TGACTTATTA CAAGAGAATG AACAGCTGAT GAAGGTAATG 
AAGACTAAAC ATGAATGTCA AAATCTAGAA TCAGAACCAA TTAGGAACTC TGTGAAAGAA 
AGAGAGAGTG AGAGAAATCA ATGTAATTTT AAACCTCAGA TGGATCTTGA AGTTAAAGAA 
ATTTCTCTAG ATAGTTATAA TGCGCAGTTG GTGCAATTAG AAGCTATGCT AAGAAATAAG 
GAATTAAAAC TTCAGGAAAG TGAGAAGGAG AAGGAGTGCC TGCAGCATGA ATTACAGACA 
ATTAGAGGAG ATCTTGAAAC CAGCAATTTG CAAGACATGC AGTCACAAGA AATTAGTGGC 
CTTAAAGACT GTGAAATAGA TGCGGAAGAA AAGTATATTT CAGGGCCTCA TGAGTTGTCA 
ACAAGTCAAA ACGACAATGC ACACCTTCAG TGCTCTCTGC AAACAACAAT GAACAAGCTG 
AATGAGCTAG AGAAAATATG TGAAATACTG CAGGCTGAAA AGTATGAACT CGTAACTGAG 
CTGAATGATT CAAGGTCAGA ATGTATCACA GCAACTAGGA AAATGGCAGA AGAGGTAGGG 
AAACTACTAA ATGAAGTTAA AATATTAAAT GATGACAGTG GTCTTCTCCA TGGTGAGTTA 
GTGGAAGACA TACCAGGAGG TGAATTTGGT GAACAACCAA ATGAACAGCA CCCTGTGTCT 
TTGGCTCCAT TGGACGAGAG TAATTCCTAC GAGCACTTGA CATTGTCAGA CAAAGAAGTT 
CAAATGCACT TTGCCGAATT GCAAGAGAAA TTCTTATCTT TACAAAGTGA ACACAAAATT 
TTACATGATC AGCACTGTCA GATGAGCTCT AAAATGTCAG AGCTGCAGAC CTATGTTGAC 
TCATTAAAGG CCGAAAATTT GGTCTTGTCA ACGAATCTGA GAAACTTTCA AGGTGACTTG 
GTGAAGGAGA TGCAGCTGGG CTTGGAGGAG GGGCTCGTTC CATCCCTGTC ATCCTCTTGT 
GTGCCTGACA GCTCTAGTCT TAGCAGTTTG GGAGACTCCT CCTTTTACAG AGCTCTTTTA 
GAACAGACAG GAGATATGTC TCTTTTGAGT AATTTAGAAG GGGCTGTTTC AGCAAACCAG 
TGCAGTGTAG ATGAAGTATT TTGCAGCAGT CTGCAGGAGG AGAATCTGAC CAGGAAAGAA 
ACCCCTTCGG CCCCAGCGAA GGGTGTTGAA GAGCTTGAGT CCCTCTGTGA GGTGTACCGG 
CAGTCCCTCG AGAAGCTAGA AGAGAAAATG GAAAGTCAAG GGATTATGAA AAATAAGGAA 
ATTCAAGAGC TCGAGCAGTT ATTAAGTTCT GAAAGGCAAG AGCTTGACTG CCTTAGGAAG 
CAGTATTTGT CAGAAAATGA ACAGTGGCAA CAGAAGCTGA CAAGCGTGAC TCTGGAGATG 
GAGTCCAAGT TGGCGGCAGA AAAGAAACAG ACGGAACAAC TGTCACTTGA GCTGGAAGTA 
GCACGACTCC AGCTACAAGG TCTGGACTTA AGTTCTCGGT CTTTGCTTGG CATCGACACA 
GAAGATGCTA TTCAAGGCCG AAATGAGAGC TGTGACATAT CAAAAGAACA TACTTCAGAA 
ACTACAGAAA GAACACCAAA GCATGATGTT CATCAGATTT GTGATAAAGA TGCTCAGCAG 
GACCTCAATC TAGACATTGA GAAAATAACT GAGACTGGTG CAGTGAAACC CACAGGAGAG 
TGCTCTGGGG AACAGTCCCC AGATACCAAT TATGAGCCTC CAGGGGAAGA TAAAACCCAG 
GGCTCTTCAG AATGCATTTC TGAATTGTCA TTTTCTGGTC CTAATGCTTT GGTACCTATG 
GATTTCCTGG GGAATCAGGA AGATATCCAT AATCTTCAAC TGCGGGTAAA AGAGACATCA 
AATGAGAATT TGAGATTACT TCATGTGATA GAGGACCGTG ACAGAAAAGT TGAAAGTTTG 
CTAAATGAAA TGAAAGAATT AGACTCAAAA CTCCATTTAC AGGAGGTACA ACTAATGACC 
AAAATTGAAG CATGCATAGA ATTGGAAAAA ATAGTTGGGG AACTTAAGAA AGAAAACTCA 
GATTTAAGTG AAAAATTGGA ATATTTTTCT TGTGATCACC AGGAGTTACT CCAGAGAGTA 
GAAACTTCTG AAGGCCTCAA TTCTGATTTA GAAATGCATG CAGATAAATC ATCACGTGAA 
GATATTGGAG ATAATGTGGC CAAGGTGAAT GACAGCTGGA AGGAGAGATT TCTTGATGTG 
GAAAATGAGC TGAGTAGGAT CAGATCGGAG AAAGCTAGCA TTGAGCATGA AGCCCTCTAC 
CTGGAGGCTG ACTTAGAGGT AGTTCAAACA GAGAAGCTAT GTTTAGAAAA AGACAATGAA 
AATAAGCAGA AGGTTATTGT CTGCCTTGAA GAAGAACTCT CAGTGGTCAC AAGTGAGAGA 
AACCAGCTTC GTGGAGAATT AGATACTATG TCAAAAAAAA CCACGGCACT GGATCAGTTG 
TCTGAAAAAA TGAAGGAGAA AACACAAGAG CTTGAGTCTC ATCAAAGTGA GTGTCTCCAT 
TGCATTCAGG TGGCAGAGGC AGAGGTGAAG GAAAAGACGG AACTCCTTCA GACTTTGTCC 
TCTGATGTGA GTGAGCTGTT AAAAGACAAA ACTCATCTCC AGGAAAAGCT GCAGAGTTTG 
GAAAAGGACT CACAGGCACT GTCTTTGACA AAATGTGAGC TGGAAAACCA AATTGCACAA 
CTGAATAAAG AGAAAGAATT GCTTGTCAAG GAATCTGAAA GCCTGCAGGC CAGACTGAGT 
GAATCAGATT ATGAAAAGCT GAATGTCTCC AAGGCCTTGG AGGCCGCACT GGTGGAGAAA 
GGTGAGTTCG CATTGAGGCT GAGCTCAACA CAGGAGGAAG TGCATCAGCT GAGAAGAGGC 
ATCGAGAAAC TGAGAGTTCG CATTGAGGCC GATGAAAAGA AGCAGCTGCA CATCGCAGAG 
AAACTGAAAG AACGCGAGCG GGAGAATGAT TCACTTAAGG ATAAAGTTGA GAACCTTGAA 
AGGGAATTGC AGATGTCAGA AGAAAACCAG GAGCTAGTGA TTCTTGATGC CGAGAATTCC 
AAAGCAGAAG TAGAGACTCT AAAAACACAA ATAGAAGAGA TGGCCAGAAG CCTGAAAGTT 
TTTGAATTAG ACCTTGTCAC GTTAAGGTCT GAAAAAGAAA ATCTGACAAA ACAAATACAA 
GAAAAACAAG GTCAGTTGTC AGAACTAGAC AAGTTACTCT CTTCATTTAA AAGTCTGTTA 
GAAGAAAAGG AGCAAGCAGA GATACAGATC AAAGAAGAAT CTAAAACTGC AGTGGAGATG 
CTTCAGAATC AGTTAAAGGA GCTAAATGAG GCAGTAGCAG CCTTGTGTGG TGACCAAGAA 
ATTATGAAGG CCACAGAACA GAGTCTAGAC CCACCAATAG AGGAAGAGCA TCAGCTGAGA 
AATAGCATTG AAAAGCTGAG AGCCCGCCTA GAAGCTGATG AAAAGAAGCA GCTCTGTGTC 
TTACAACAAC TGAAGGAAAG TGAGCATCAT GCAGATTTAC TTAAGGGTAG AGTGGAGAAC 
CTTGAAAGAG AGCTAGAGAT AGCCAGGACA AACCAAGAGC ATGCAGCTCT TGAGGCAGAG 
AATTCCAAAG GAGAGGTAGA GACCCTAAAA GCAAAAATAG AAGGGATGAC CCAAAGTCTG 
AGAGGTCTGG AATTAGATGT TGTTACTATA AGGTCAGAAA AAGAAAATCT GACAAATGAA 
TTACAAAAAG AGCAAGAGCG AATATCTGAA TTAGAAATAA TAAATTCATC ATTTGAAAAT 
ATTTTGCAAG AAAAAGAGCA AGAGAAAGTA CAGATGAAAG AAAAATCAAG CACTGCCATG 
GAGATGCTTC AAACACAATT AAAAGAGCTC AATGAGAGAG TGGCAGCCCT GCATAATGAC 
CAAGAAGCCT GTAAGGCCAA AGAGCAGAAT CTTAGTAGTC AAGTAGAGTG TCTTGAACTT 
GAGAAGGCTC AGTTGCTACA AGGCCTTGAT GAGGCCAAAA ATAATTATAT TGTTTTGCAA 
TCTTCAGTGA ATGGCCTCAT TCAAGAAGTA GAAGATGGCA AGCAGAAACT GGAGAAGAAG 
GATGAAGAAA TCAGTAGACT GAAAAATCAA ATTCAAGACC AAGAGCAGCT TGTCTCTAAA 
CTGTCCCAGG TGGAAGGAGA GCACCAACTT TGGAAGGAGC AAAACTTAGA ACTGAGAAAT 
CTGACAGTGG AATTGGAGCA GAAGATCCAA GTGCTACAAT CCAAAAATGC CTCTTTGCAG 
GACACATTAG AAGTGCTGCA GAGTTCTTAC AAGAATCTAG AGAATGAGCT TGAATTGACA 
AAAATGGACA AAATGTCCTT TGTTGAAAAA GTAAACAAAA TGACTGCAAA GGAAACTGAG 
CTGCAGAGGG AAATGCATGA GATGGCACAG AAAACAGCAG AGCTGCAAGA AGAACTCAGT 
GGAGAGAAAA ATAGGCTAGC TGGAGAGTTG CAGTTACTGT TGGAAGAAAT AAAGAGCAGC 
AAAGATCAAT TGAAGGAGCT CACACTAGAA AATAGTGAAT TGAAGAAGAG CCTAGATTGC 
ATGCACAAAG ACCAGGTGGA AAAGGAAGGG AAAGTGAGAG AGGAAATAGC TGAATATCAG 
CTACGGCTTC ATGAAGCTGA AAAGAAACAC CAGGCTTTGC TTTTGGACAC AAACAAACAG 
TATGAAGTAG AAATCCAGAC ATACCGAGAG AAATTGACTT CTAAAGAAGA ATGTCTCAGT 
TCACAGAAGC TGGAGATAGA CCTTTTAAAG TCTAGTAAAG AAGAGCTCAA TAATTCATTG 
AAAGCTACTA CTCAGATTTT GGAAGAATTG AAGAAAACCA AGATGGACAA TCTAAAATAT 
GTAAATCAGT TGAAGAAGGA AAATGAACGT GCCCAGGGGA AAATGAAGTT GTTGATCAAA 
TCCTGTAAAC AGCTGGAAGA GGAAAAGGAG ATACTGCAGA AAGAACTCTC TCAACTTCAA 
GCTGCACAGG AGAAGCAGAA AACAGGTACT GTTATGGATA CCAAGGTCGA TGAATTAACA 
ACTGAGATCA AAGAACTGAA AGAAACTCTT GAAGAAAAAA CCAAGGAGGC AGATGAATAC 
TTGGATAAGT ACTGTTCCTT GCTTATAAGC CATGAAAAGT TAGAGAAAGC TAAAGAGATG 
TTAGAGACAC AAGTGGCCCA TCTGTGTTCA CAGCAATCTA AACAAGATTC CCGAGGGTCT 
CCTTTGCTAG GTCCAGTTGT TCCAGGACCA TCTCCAATCC CTTCTGTTAC TGAAAAGAGG 
TTATCATCTG GCCAAAATAA AGCTTCAGGC AAGAGGCAAA GATCCAGTGG AATATGGGAG 
AATGGTAGAG GACCAACACC TGCTACCCCA GAGAGCTTTT CTAAAAAAAG CAAGAAAGCA 
GTCATGAGTG GTATTCACCC TGCAGAAGAC ACGGAAGGTA CTGAGTTTGA GCCAGAGGGA 
CTTCCAGAAG TTGTAAAGAA AGGGTTTGCT GACATCCCGA CAGGAAAGAC TAGCCCATAT 
ATCCTGCGAA GAACAACCAT GGCAACTCGG ACCAGCCCCC GCCTGGCTGC ACAGAAGTTA 
GCGCTATCCC CACTGAGTCT CGGCAAAGAA AATCTTGCAG AGTCCTCCAA ACCAACAGCT 
GGTGGCAGCA GATCACAAAA GGTCAAAGTT GCTCAGCGGA GCCCAGTAGA TTCAGGCACC 
ATCCTCCGAG AACCCACCAC GAAATCCGTC CCAGTCAATA ATCTTCCTGA GAGAAGTCCG 
ACTGACAGCC CCAGAGAGGG CCTGAGGGTC AAGCGAGGCC GACTTGTCCC CAGCCCCAAA 
GCTGGACTGG AGTCCAACGG CAGTGAGAAC TGTAAGGTCC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CENPF ( XM_017000086.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016855575.1 Homo sapiens centromere protein F (CENPF), transcript variant X1, mRNA.

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CloneID	OHu14151
Clone ID Related Accession (Same CDS sequence)	XM_017000086.1 , NM_016343.4 , NM_016343.3 , XM_017000086.2
Accession Version	XM_017000086.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9345bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	centromere protein F isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTGGG CTTTGGAAGA ATGGAAAGAA GGGCTGCCTA CAAGAGCTCT TCAGAAAATT 
CAAGAGCTTG AAGGACAGCT TGACAAACTG AAGAAGGAAA AGCAGCAAAG GCAGTTTCAG 
CTTGACAGTC TCGAGGCTGC GCTGCAGAAG CAAAAACAGA AGGTTGAAAA TGAAAAAACC 
GAGGGTACAA ACCTGAAAAG GGAGAATCAA AGATTGATGG AAATATGTGA AAGTCTGGAG 
AAAACTAAGC AGAAGATTTC TCATGAACTT CAAGTCAAGG AGTCACAAGT GAATTTCCAG 
GAAGGACAAC TGAATTCAGG CAAAAAACAA ATAGAAAAAC TGGAACAGGA ACTTAAAAGG 
TGTAAATCTG AGCTTGAAAG AAGCCAACAA GCTGCGCAGT CTGCAGATGT CTCTCTGAAT 
CCATGCAATA CACCACAAAA AATTTTTACA ACTCCACTAA CACCAAGTCA ATATTATAGT 
GGTTCCAAGT ATGAAGATCT AAAAGAAAAA TATAATAAAG AGGTTGAAGA ACGAAAAAGA 
TTAGAGGCAG AGGTTAAAGC CTTGCAGGCT AAAAAAGCAA GCCAGACTCT TCCACAAGCC 
ACCATGAATC ACCGCGACAT TGCCCGGCAT CAGGCTTCAT CATCTGTGTT CTCATGGCAG 
CAAGAGAAGA CCCCAAGTCA TCTTTCATCT AATTCTCAAA GAACTCCAAT TAGGAGAGAT 
TTCTCTGCAT CTTACTTTTC TGGGGAACAA GAGGTGACTC CAAGTCGATC AACTTTGCAA 
ATAGGGAAAA GAGATGCTAA TAGCAGTTTC TTTGACAATT CTAGCAGTCC TCATCTTTTG 
GATCAATTAA AAGCGCAGAA TCAAGAGCTA AGAAACAAGA TTAATGAGTT GGAACTACGC 
CTGCAAGGAC ATGAAAAAGA AATGAAAGGC CAAGTGAATA AGTTTCAAGA ACTCCAACTC 
CAACTGGAGA AAGCAAAAGT GGAATTAATT GAAAAAGAGA AAGTTTTGAA CAAATGTAGG 
GATGAACTAG TGAGAACAAC AGCACAATAC GACCAGGCGT CAACCAAGTA TACTGCATTG 
GAACAAAAAC TGAAAAAATT GACGGAAGAT TTGAGTTGTC AGCGACAAAA TGCAGAAAGT 
GCCAGATGTT CTCTGGAACA GAAAATTAAG GAAAAAGAAA AGGAGTTTCA AGAGGAGCTC 
TCCCGTCAAC AGCGTTCTTT CCAAACACTG GACCAGGAGT GCATCCAGAT GAAGGCCAGA 
CTCACCCAGG AGTTACAGCA AGCCAAGAAT ATGCACAACG TCCTGCAGGC TGAACTGGAT 
AAACTCACAT CAGTAAAGCA ACAGCTAGAA AACAATTTGG AAGAGTTTAA GCAAAAGTTG 
TGCAGAGCTG AACAGGCGTT CCAGGCGAGT CAGATCAAGG AGAATGAGCT GAGGAGAAGC 
ATGGAGGAAA TGAAGAAGGA AAACAACCTC CTTAAGAGTC ACTCTGAGCA AAAGGCCAGA 
GAAGTCTGCC ACCTGGAGGC AGAACTCAAG AACATCAAAC AGTGTTTAAA TCAGAGCCAG 
AATTTTGCAG AAGAAATGAA AGCGAAGAAT ACCTCTCAGG AAACCATGTT AAGAGATCTT 
CAAGAAAAAA TAAATCAGCA AGAAAACTCC TTGACTTTAG AAAAACTGAA GCTTGCTGTG 
GCTGATCTGG AAAAGCAGCG AGATTGTTCT CAAGACCTTT TGAAGAAAAG AGAACATCAC 
ATTGAACAAC TTAATGATAA GTTAAGCAAG ACAGAGAAAG AGTCCAAAGC CTTGCTGAGT 
GCTTTAGAGT TAAAAAAGAA AGAATATGAA GAATTGAAAG AAGAGAAAAC TCTGTTTTCT 
TGTTGGAAAA GTGAAAACGA AAAACTTTTA ACTCAGATGG AATCAGAAAA GGAAAACTTG 
CAGAGTAAAA TTAATCACTT GGAAACTTGT CTGAAGACAC AGCAAATAAA AAGTCATGAA 
TACAACGAGA GAGTAAGAAC GCTGGAGATG GACAGAGAAA ACCTAAGTGT CGAGATCAGA 
AACCTTCACA ACGTGTTAGA CAGTAAGTCA GTGGAGGTAG AGACCCAGAA ACTAGCTTAT 
ATGGAGCTAC AGCAGAAAGC TGAGTTCTCA GATCAGAAAC ATCAGAAGGA AATAGAAAAT 
ATGTGTTTGA AGACTTCTCA GCTTACTGGG CAAGTTGAAG ATCTAGAACA CAAGCTTCAG 
TTACTGTCAA ATGAAATAAT GGACAAAGAC CGGTGTTACC AAGACTTGCA TGCCGAATAT 
GAGAGCCTCA GGGATCTGCT AAAATCCAAA GATGCTTCTC TGGTGACAAA TGAAGATCAT 
CAGAGAAGTC TTTTGGCTTT TGATCAGCAG CCTGCCATGC ATCATTCCTT TGCAAATATA 
ATTGGAGAAC AAGGAAGCAT GCCTTCAGAG AGGAGTGAAT GTCGTTTAGA AGCAGACCAA 
AGTCCGAAAA ATTCTGCCAT CCTACAAAAT AGAGTTGATT CACTTGAATT TTCATTAGAG 
TCTCAAAAAC AGATGAACTC AGACCTGCAA AAGCAGTGTG AAGAGTTGGT GCAAATCAAA 
GGAGAAATAG AAGAAAATCT CATGAAAGCA GAACAGATGC ATCAAAGTTT TGTGGCTGAA 
ACAAGTCAGC GCATTAGTAA GTTACAGGAA GACACTTCTG CTCACCAGAA TGTTGTTGCT 
GAAACCTTAA GTGCCCTTGA GAACAAGGAA AAAGAGCTGC AACTTTTAAA TGATAAGGTA 
GAAACTGAGC AGGCAGAGAT TCAAGAATTA AAAAAGAGCA ACCATCTACT TGAAGACTCT 
CTAAAGGAGC TACAACTTTT ATCCGAAACC CTAAGCTTGG AGAAGAAAGA AATGAGTTCC 
ATCATTTCTC TAAATAAAAG GGAAATTGAA GAGCTGACCC AAGAGAATGG GACTCTTAAG 
GAAATTAATG CATCCTTAAA TCAAGAGAAG ATGAACTTAA TCCAGAAAAG TGAGAGTTTT 
GCAAACTATA TAGATGAAAG GGAGAAAAGC ATTTCAGAGT TATCTGATCA GTACAAGCAA 
GAAAAACTTA TTTTACTACA AAGATGTGAA GAAACCGGAA ATGCATATGA GGATCTTAGT 
CAAAAATACA AAGCAGCACA GGAAAAGAAT TCTAAATTAG AATGCTTGCT AAATGAATGC 
ACTAGTCTTT GTGAAAATAG GAAAAATGAG TTGGAACAGC TAAAGGAAGC ATTTGCAAAG 
GAACACCAAG AATTCTTAAC AAAATTAGCA TTTGCTGAAG AAAGAAATCA GAATCTGATG 
CTAGAGTTGG AGACAGTGCA GCAAGCTCTG AGATCTGAGA TGACAGATAA CCAAAACAAT 
TCTAAGAGCG AGGCTGGTGG TTTAAAGCAA GAAATCATGA CTTTAAAGGA AGAACAAAAC 
AAAATGCAAA AGGAAGTTAA TGACTTATTA CAAGAGAATG AACAGCTGAT GAAGGTAATG 
AAGACTAAAC ATGAATGTCA AAATCTAGAA TCAGAACCAA TTAGGAACTC TGTGAAAGAA 
AGAGAGAGTG AGAGAAATCA ATGTAATTTT AAACCTCAGA TGGATCTTGA AGTTAAAGAA 
ATTTCTCTAG ATAGTTATAA TGCGCAGTTG GTGCAATTAG AAGCTATGCT AAGAAATAAG 
GAATTAAAAC TTCAGGAAAG TGAGAAGGAG AAGGAGTGCC TGCAGCATGA ATTACAGACA 
ATTAGAGGAG ATCTTGAAAC CAGCAATTTG CAAGACATGC AGTCACAAGA AATTAGTGGC 
CTTAAAGACT GTGAAATAGA TGCGGAAGAA AAGTATATTT CAGGGCCTCA TGAGTTGTCA 
ACAAGTCAAA ACGACAATGC ACACCTTCAG TGCTCTCTGC AAACAACAAT GAACAAGCTG 
AATGAGCTAG AGAAAATATG TGAAATACTG CAGGCTGAAA AGTATGAACT CGTAACTGAG 
CTGAATGATT CAAGGTCAGA ATGTATCACA GCAACTAGGA AAATGGCAGA AGAGGTAGGG 
AAACTACTAA ATGAAGTTAA AATATTAAAT GATGACAGTG GTCTTCTCCA TGGTGAGTTA 
GTGGAAGACA TACCAGGAGG TGAATTTGGT GAACAACCAA ATGAACAGCA CCCTGTGTCT 
TTGGCTCCAT TGGACGAGAG TAATTCCTAC GAGCACTTGA CATTGTCAGA CAAAGAAGTT 
CAAATGCACT TTGCCGAATT GCAAGAGAAA TTCTTATCTT TACAAAGTGA ACACAAAATT 
TTACATGATC AGCACTGTCA GATGAGCTCT AAAATGTCAG AGCTGCAGAC CTATGTTGAC 
TCATTAAAGG CCGAAAATTT GGTCTTGTCA ACGAATCTGA GAAACTTTCA AGGTGACTTG 
GTGAAGGAGA TGCAGCTGGG CTTGGAGGAG GGGCTCGTTC CATCCCTGTC ATCCTCTTGT 
GTGCCTGACA GCTCTAGTCT TAGCAGTTTG GGAGACTCCT CCTTTTACAG AGCTCTTTTA 
GAACAGACAG GAGATATGTC TCTTTTGAGT AATTTAGAAG GGGCTGTTTC AGCAAACCAG 
TGCAGTGTAG ATGAAGTATT TTGCAGCAGT CTGCAGGAGG AGAATCTGAC CAGGAAAGAA 
ACCCCTTCGG CCCCAGCGAA GGGTGTTGAA GAGCTTGAGT CCCTCTGTGA GGTGTACCGG 
CAGTCCCTCG AGAAGCTAGA AGAGAAAATG GAAAGTCAAG GGATTATGAA AAATAAGGAA 
ATTCAAGAGC TCGAGCAGTT ATTAAGTTCT GAAAGGCAAG AGCTTGACTG CCTTAGGAAG 
CAGTATTTGT CAGAAAATGA ACAGTGGCAA CAGAAGCTGA CAAGCGTGAC TCTGGAGATG 
GAGTCCAAGT TGGCGGCAGA AAAGAAACAG ACGGAACAAC TGTCACTTGA GCTGGAAGTA 
GCACGACTCC AGCTACAAGG TCTGGACTTA AGTTCTCGGT CTTTGCTTGG CATCGACACA 
GAAGATGCTA TTCAAGGCCG AAATGAGAGC TGTGACATAT CAAAAGAACA TACTTCAGAA 
ACTACAGAAA GAACACCAAA GCATGATGTT CATCAGATTT GTGATAAAGA TGCTCAGCAG 
GACCTCAATC TAGACATTGA GAAAATAACT GAGACTGGTG CAGTGAAACC CACAGGAGAG 
TGCTCTGGGG AACAGTCCCC AGATACCAAT TATGAGCCTC CAGGGGAAGA TAAAACCCAG 
GGCTCTTCAG AATGCATTTC TGAATTGTCA TTTTCTGGTC CTAATGCTTT GGTACCTATG 
GATTTCCTGG GGAATCAGGA AGATATCCAT AATCTTCAAC TGCGGGTAAA AGAGACATCA 
AATGAGAATT TGAGATTACT TCATGTGATA GAGGACCGTG ACAGAAAAGT TGAAAGTTTG 
CTAAATGAAA TGAAAGAATT AGACTCAAAA CTCCATTTAC AGGAGGTACA ACTAATGACC 
AAAATTGAAG CATGCATAGA ATTGGAAAAA ATAGTTGGGG AACTTAAGAA AGAAAACTCA 
GATTTAAGTG AAAAATTGGA ATATTTTTCT TGTGATCACC AGGAGTTACT CCAGAGAGTA 
GAAACTTCTG AAGGCCTCAA TTCTGATTTA GAAATGCATG CAGATAAATC ATCACGTGAA 
GATATTGGAG ATAATGTGGC CAAGGTGAAT GACAGCTGGA AGGAGAGATT TCTTGATGTG 
GAAAATGAGC TGAGTAGGAT CAGATCGGAG AAAGCTAGCA TTGAGCATGA AGCCCTCTAC 
CTGGAGGCTG ACTTAGAGGT AGTTCAAACA GAGAAGCTAT GTTTAGAAAA AGACAATGAA 
AATAAGCAGA AGGTTATTGT CTGCCTTGAA GAAGAACTCT CAGTGGTCAC AAGTGAGAGA 
AACCAGCTTC GTGGAGAATT AGATACTATG TCAAAAAAAA CCACGGCACT GGATCAGTTG 
TCTGAAAAAA TGAAGGAGAA AACACAAGAG CTTGAGTCTC ATCAAAGTGA GTGTCTCCAT 
TGCATTCAGG TGGCAGAGGC AGAGGTGAAG GAAAAGACGG AACTCCTTCA GACTTTGTCC 
TCTGATGTGA GTGAGCTGTT AAAAGACAAA ACTCATCTCC AGGAAAAGCT GCAGAGTTTG 
GAAAAGGACT CACAGGCACT GTCTTTGACA AAATGTGAGC TGGAAAACCA AATTGCACAA 
CTGAATAAAG AGAAAGAATT GCTTGTCAAG GAATCTGAAA GCCTGCAGGC CAGACTGAGT 
GAATCAGATT ATGAAAAGCT GAATGTCTCC AAGGCCTTGG AGGCCGCACT GGTGGAGAAA 
GGTGAGTTCG CATTGAGGCT GAGCTCAACA CAGGAGGAAG TGCATCAGCT GAGAAGAGGC 
ATCGAGAAAC TGAGAGTTCG CATTGAGGCC GATGAAAAGA AGCAGCTGCA CATCGCAGAG 
AAACTGAAAG AACGCGAGCG GGAGAATGAT TCACTTAAGG ATAAAGTTGA GAACCTTGAA 
AGGGAATTGC AGATGTCAGA AGAAAACCAG GAGCTAGTGA TTCTTGATGC CGAGAATTCC 
AAAGCAGAAG TAGAGACTCT AAAAACACAA ATAGAAGAGA TGGCCAGAAG CCTGAAAGTT 
TTTGAATTAG ACCTTGTCAC GTTAAGGTCT GAAAAAGAAA ATCTGACAAA ACAAATACAA 
GAAAAACAAG GTCAGTTGTC AGAACTAGAC AAGTTACTCT CTTCATTTAA AAGTCTGTTA 
GAAGAAAAGG AGCAAGCAGA GATACAGATC AAAGAAGAAT CTAAAACTGC AGTGGAGATG 
CTTCAGAATC AGTTAAAGGA GCTAAATGAG GCAGTAGCAG CCTTGTGTGG TGACCAAGAA 
ATTATGAAGG CCACAGAACA GAGTCTAGAC CCACCAATAG AGGAAGAGCA TCAGCTGAGA 
AATAGCATTG AAAAGCTGAG AGCCCGCCTA GAAGCTGATG AAAAGAAGCA GCTCTGTGTC 
TTACAACAAC TGAAGGAAAG TGAGCATCAT GCAGATTTAC TTAAGGGTAG AGTGGAGAAC 
CTTGAAAGAG AGCTAGAGAT AGCCAGGACA AACCAAGAGC ATGCAGCTCT TGAGGCAGAG 
AATTCCAAAG GAGAGGTAGA GACCCTAAAA GCAAAAATAG AAGGGATGAC CCAAAGTCTG 
AGAGGTCTGG AATTAGATGT TGTTACTATA AGGTCAGAAA AAGAAAATCT GACAAATGAA 
TTACAAAAAG AGCAAGAGCG AATATCTGAA TTAGAAATAA TAAATTCATC ATTTGAAAAT 
ATTTTGCAAG AAAAAGAGCA AGAGAAAGTA CAGATGAAAG AAAAATCAAG CACTGCCATG 
GAGATGCTTC AAACACAATT AAAAGAGCTC AATGAGAGAG TGGCAGCCCT GCATAATGAC 
CAAGAAGCCT GTAAGGCCAA AGAGCAGAAT CTTAGTAGTC AAGTAGAGTG TCTTGAACTT 
GAGAAGGCTC AGTTGCTACA AGGCCTTGAT GAGGCCAAAA ATAATTATAT TGTTTTGCAA 
TCTTCAGTGA ATGGCCTCAT TCAAGAAGTA GAAGATGGCA AGCAGAAACT GGAGAAGAAG 
GATGAAGAAA TCAGTAGACT GAAAAATCAA ATTCAAGACC AAGAGCAGCT TGTCTCTAAA 
CTGTCCCAGG TGGAAGGAGA GCACCAACTT TGGAAGGAGC AAAACTTAGA ACTGAGAAAT 
CTGACAGTGG AATTGGAGCA GAAGATCCAA GTGCTACAAT CCAAAAATGC CTCTTTGCAG 
GACACATTAG AAGTGCTGCA GAGTTCTTAC AAGAATCTAG AGAATGAGCT TGAATTGACA 
AAAATGGACA AAATGTCCTT TGTTGAAAAA GTAAACAAAA TGACTGCAAA GGAAACTGAG 
CTGCAGAGGG AAATGCATGA GATGGCACAG AAAACAGCAG AGCTGCAAGA AGAACTCAGT 
GGAGAGAAAA ATAGGCTAGC TGGAGAGTTG CAGTTACTGT TGGAAGAAAT AAAGAGCAGC 
AAAGATCAAT TGAAGGAGCT CACACTAGAA AATAGTGAAT TGAAGAAGAG CCTAGATTGC 
ATGCACAAAG ACCAGGTGGA AAAGGAAGGG AAAGTGAGAG AGGAAATAGC TGAATATCAG 
CTACGGCTTC ATGAAGCTGA AAAGAAACAC CAGGCTTTGC TTTTGGACAC AAACAAACAG 
TATGAAGTAG AAATCCAGAC ATACCGAGAG AAATTGACTT CTAAAGAAGA ATGTCTCAGT 
TCACAGAAGC TGGAGATAGA CCTTTTAAAG TCTAGTAAAG AAGAGCTCAA TAATTCATTG 
AAAGCTACTA CTCAGATTTT GGAAGAATTG AAGAAAACCA AGATGGACAA TCTAAAATAT 
GTAAATCAGT TGAAGAAGGA AAATGAACGT GCCCAGGGGA AAATGAAGTT GTTGATCAAA 
TCCTGTAAAC AGCTGGAAGA GGAAAAGGAG ATACTGCAGA AAGAACTCTC TCAACTTCAA 
GCTGCACAGG AGAAGCAGAA AACAGGTACT GTTATGGATA CCAAGGTCGA TGAATTAACA 
ACTGAGATCA AAGAACTGAA AGAAACTCTT GAAGAAAAAA CCAAGGAGGC AGATGAATAC 
TTGGATAAGT ACTGTTCCTT GCTTATAAGC CATGAAAAGT TAGAGAAAGC TAAAGAGATG 
TTAGAGACAC AAGTGGCCCA TCTGTGTTCA CAGCAATCTA AACAAGATTC CCGAGGGTCT 
CCTTTGCTAG GTCCAGTTGT TCCAGGACCA TCTCCAATCC CTTCTGTTAC TGAAAAGAGG 
TTATCATCTG GCCAAAATAA AGCTTCAGGC AAGAGGCAAA GATCCAGTGG AATATGGGAG 
AATGGTAGAG GACCAACACC TGCTACCCCA GAGAGCTTTT CTAAAAAAAG CAAGAAAGCA 
GTCATGAGTG GTATTCACCC TGCAGAAGAC ACGGAAGGTA CTGAGTTTGA GCCAGAGGGA 
CTTCCAGAAG TTGTAAAGAA AGGGTTTGCT GACATCCCGA CAGGAAAGAC TAGCCCATAT 
ATCCTGCGAA GAACAACCAT GGCAACTCGG ACCAGCCCCC GCCTGGCTGC ACAGAAGTTA 
GCGCTATCCC CACTGAGTCT CGGCAAAGAA AATCTTGCAG AGTCCTCCAA ACCAACAGCT 
GGTGGCAGCA GATCACAAAA GGTCAAAGTT GCTCAGCGGA GCCCAGTAGA TTCAGGCACC 
ATCCTCCGAG AACCCACCAC GAAATCCGTC CCAGTCAATA ATCTTCCTGA GAGAAGTCCG 
ACTGACAGCC CCAGAGAGGG CCTGAGGGTC AAGCGAGGCC GACTTGTCCC CAGCCCCAAA 
GCTGGACTGG AGTCCAACGG CAGTGAGAAC TGTAAGGTCC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016855575.1
CDS	162..9506
Translation	MSWALEEWKEGLPTRALQKIQELEGQLDKLKKEKQQRQFQLDSLEAALQKQKQKVENEKTEGTNLKRENQRLMEICESLEKTKQKISHELQVKESQVNFQEGQLNSGKKQIEKLEQELKRCKSELERSQQAAQSADVSLNPCNTPQKIFTTPLTPSQYYSGSKYEDLKEKYNKEVEERKRLEAEVKALQAKKASQTLPQATMNHRDIARHQASSSVFSWQQEKTPSHLSSNSQRTPIRRDFSASYFSGEQEVTPSRSTLQIGKRDANSSFFDNSSSPHLLDQLKAQNQELRNKINELELRLQGHEKEMKGQVNKFQELQLQLEKAKVELIEKEKVLNKCRDELVRTTAQYDQASTKYTALEQKLKKLTEDLSCQRQNAESARCSLEQKIKEKEKEFQEELSRQQRSFQTLDQECIQMKARLTQELQQAKNMHNVLQAELDKLTSVKQQLENNLEEFKQKLCRAEQAFQASQIKENELRRSMEEMKKENNLLKSHSEQKAREVCHLEAELKNIKQCLNQSQNFAEEMKAKNTSQETMLRDLQEKINQQENSLTLEKLKLAVADLEKQRDCSQDLLKKREHHIEQLNDKLSKTEKESKALLSALELKKKEYEELKEEKTLFSCWKSENEKLLTQMESEKENLQSKINHLETCLKTQQIKSHEYNERVRTLEMDRENLSVEIRNLHNVLDSKSVEVETQKLAYMELQQKAEFSDQKHQKEIENMCLKTSQLTGQVEDLEHKLQLLSNEIMDKDRCYQDLHAEYESLRDLLKSKDASLVTNEDHQRSLLAFDQQPAMHHSFANIIGEQGSMPSERSECRLEADQSPKNSAILQNRVDSLEFSLESQKQMNSDLQKQCEELVQIKGEIEENLMKAEQMHQSFVAETSQRISKLQEDTSAHQNVVAETLSALENKEKELQLLNDKVETEQAEIQELKKSNHLLEDSLKELQLLSETLSLEKKEMSSIISLNKREIEELTQENGTLKEINASLNQEKMNLIQKSESFANYIDEREKSISELSDQYKQEKLILLQRCEETGNAYEDLSQKYKAAQEKNSKLECLLNECTSLCENRKNELEQLKEAFAKEHQEFLTKLAFAEERNQNLMLELETVQQALRSEMTDNQNNSKSEAGGLKQEIMTLKEEQNKMQKEVNDLLQENEQLMKVMKTKHECQNLESEPIRNSVKERESERNQCNFKPQMDLEVKEISLDSYNAQLVQLEAMLRNKELKLQESEKEKECLQHELQTIRGDLETSNLQDMQSQEISGLKDCEIDAEEKYISGPHELSTSQNDNAHLQCSLQTTMNKLNELEKICEILQAEKYELVTELNDSRSECITATRKMAEEVGKLLNEVKILNDDSGLLHGELVEDIPGGEFGEQPNEQHPVSLAPLDESNSYEHLTLSDKEVQMHFAELQEKFLSLQSEHKILHDQHCQMSSKMSELQTYVDSLKAENLVLSTNLRNFQGDLVKEMQLGLEEGLVPSLSSSCVPDSSSLSSLGDSSFYRALLEQTGDMSLLSNLEGAVSANQCSVDEVFCSSLQEENLTRKETPSAPAKGVEELESLCEVYRQSLEKLEEKMESQGIMKNKEIQELEQLLSSERQELDCLRKQYLSENEQWQQKLTSVTLEMESKLAAEKKQTEQLSLELEVARLQLQGLDLSSRSLLGIDTEDAIQGRNESCDISKEHTSETTERTPKHDVHQICDKDAQQDLNLDIEKITETGAVKPTGECSGEQSPDTNYEPPGEDKTQGSSECISELSFSGPNALVPMDFLGNQEDIHNLQLRVKETSNENLRLLHVIEDRDRKVESLLNEMKELDSKLHLQEVQLMTKIEACIELEKIVGELKKENSDLSEKLEYFSCDHQELLQRVETSEGLNSDLEMHADKSSREDIGDNVAKVNDSWKERFLDVENELSRIRSEKASIEHEALYLEADLEVVQTEKLCLEKDNENKQKVIVCLEEELSVVTSERNQLRGELDTMSKKTTALDQLSEKMKEKTQELESHQSECLHCIQVAEAEVKEKTELLQTLSSDVSELLKDKTHLQEKLQSLEKDSQALSLTKCELENQIAQLNKEKELLVKESESLQARLSESDYEKLNVSKALEAALVEKGEFALRLSSTQEEVHQLRRGIEKLRVRIEADEKKQLHIAEKLKERERENDSLKDKVENLERELQMSEENQELVILDAENSKAEVETLKTQIEEMARSLKVFELDLVTLRSEKENLTKQIQEKQGQLSELDKLLSSFKSLLEEKEQAEIQIKEESKTAVEMLQNQLKELNEAVAALCGDQEIMKATEQSLDPPIEEEHQLRNSIEKLRARLEADEKKQLCVLQQLKESEHHADLLKGRVENLERELEIARTNQEHAALEAENSKGEVETLKAKIEGMTQSLRGLELDVVTIRSEKENLTNELQKEQERISELEIINSSFENILQEKEQEKVQMKEKSSTAMEMLQTQLKELNERVAALHNDQEACKAKEQNLSSQVECLELEKAQLLQGLDEAKNNYIVLQSSVNGLIQEVEDGKQKLEKKDEEISRLKNQIQDQEQLVSKLSQVEGEHQLWKEQNLELRNLTVELEQKIQVLQSKNASLQDTLEVLQSSYKNLENELELTKMDKMSFVEKVNKMTAKETELQREMHEMAQKTAELQEELSGEKNRLAGELQLLLEEIKSSKDQLKELTLENSELKKSLDCMHKDQVEKEGKVREEIAEYQLRLHEAEKKHQALLLDTNKQYEVEIQTYREKLTSKEECLSSQKLEIDLLKSSKEELNNSLKATTQILEELKKTKMDNLKYVNQLKKENERAQGKMKLLIKSCKQLEEEKEILQKELSQLQAAQEKQKTGTVMDTKVDELTTEIKELKETLEEKTKEADEYLDKYCSLLISHEKLEKAKEMLETQVAHLCSQQSKQDSRGSPLLGPVVPGPSPIPSVTEKRLSSGQNKASGKRQRSSGIWENGRGPTPATPESFSKKSKKAVMSGIHPAEDTEGTEFEPEGLPEVVKKGFADIPTGKTSPYILRRTTMATRTSPRLAAQKLALSPLSLGKENLAESSKPTAGGSRSQKVKVAQRSPVDSGTILREPTTKSVPVNNLPERSPTDSPREGLRVKRGRLVPSPKAGLESNGSENCKVQ

Target ORF information:

RefSeq Version	XM_017000086.1
Organism	Homo sapiens(human)
Definition	Homo sapiens centromere protein F (CENPF), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017000086.1

ORF Insert Sequence:

ATGAGCTGGG CTTTGGAAGA ATGGAAAGAA GGGCTGCCTA CAAGAGCTCT TCAGAAAATT 
CAAGAGCTTG AAGGACAGCT TGACAAACTG AAGAAGGAAA AGCAGCAAAG GCAGTTTCAG 
CTTGACAGTC TCGAGGCTGC GCTGCAGAAG CAAAAACAGA AGGTTGAAAA TGAAAAAACC 
GAGGGTACAA ACCTGAAAAG GGAGAATCAA AGATTGATGG AAATATGTGA AAGTCTGGAG 
AAAACTAAGC AGAAGATTTC TCATGAACTT CAAGTCAAGG AGTCACAAGT GAATTTCCAG 
GAAGGACAAC TGAATTCAGG CAAAAAACAA ATAGAAAAAC TGGAACAGGA ACTTAAAAGG 
TGTAAATCTG AGCTTGAAAG AAGCCAACAA GCTGCGCAGT CTGCAGATGT CTCTCTGAAT 
CCATGCAATA CACCACAAAA AATTTTTACA ACTCCACTAA CACCAAGTCA ATATTATAGT 
GGTTCCAAGT ATGAAGATCT AAAAGAAAAA TATAATAAAG AGGTTGAAGA ACGAAAAAGA 
TTAGAGGCAG AGGTTAAAGC CTTGCAGGCT AAAAAAGCAA GCCAGACTCT TCCACAAGCC 
ACCATGAATC ACCGCGACAT TGCCCGGCAT CAGGCTTCAT CATCTGTGTT CTCATGGCAG 
CAAGAGAAGA CCCCAAGTCA TCTTTCATCT AATTCTCAAA GAACTCCAAT TAGGAGAGAT 
TTCTCTGCAT CTTACTTTTC TGGGGAACAA GAGGTGACTC CAAGTCGATC AACTTTGCAA 
ATAGGGAAAA GAGATGCTAA TAGCAGTTTC TTTGACAATT CTAGCAGTCC TCATCTTTTG 
GATCAATTAA AAGCGCAGAA TCAAGAGCTA AGAAACAAGA TTAATGAGTT GGAACTACGC 
CTGCAAGGAC ATGAAAAAGA AATGAAAGGC CAAGTGAATA AGTTTCAAGA ACTCCAACTC 
CAACTGGAGA AAGCAAAAGT GGAATTAATT GAAAAAGAGA AAGTTTTGAA CAAATGTAGG 
GATGAACTAG TGAGAACAAC AGCACAATAC GACCAGGCGT CAACCAAGTA TACTGCATTG 
GAACAAAAAC TGAAAAAATT GACGGAAGAT TTGAGTTGTC AGCGACAAAA TGCAGAAAGT 
GCCAGATGTT CTCTGGAACA GAAAATTAAG GAAAAAGAAA AGGAGTTTCA AGAGGAGCTC 
TCCCGTCAAC AGCGTTCTTT CCAAACACTG GACCAGGAGT GCATCCAGAT GAAGGCCAGA 
CTCACCCAGG AGTTACAGCA AGCCAAGAAT ATGCACAACG TCCTGCAGGC TGAACTGGAT 
AAACTCACAT CAGTAAAGCA ACAGCTAGAA AACAATTTGG AAGAGTTTAA GCAAAAGTTG 
TGCAGAGCTG AACAGGCGTT CCAGGCGAGT CAGATCAAGG AGAATGAGCT GAGGAGAAGC 
ATGGAGGAAA TGAAGAAGGA AAACAACCTC CTTAAGAGTC ACTCTGAGCA AAAGGCCAGA 
GAAGTCTGCC ACCTGGAGGC AGAACTCAAG AACATCAAAC AGTGTTTAAA TCAGAGCCAG 
AATTTTGCAG AAGAAATGAA AGCGAAGAAT ACCTCTCAGG AAACCATGTT AAGAGATCTT 
CAAGAAAAAA TAAATCAGCA AGAAAACTCC TTGACTTTAG AAAAACTGAA GCTTGCTGTG 
GCTGATCTGG AAAAGCAGCG AGATTGTTCT CAAGACCTTT TGAAGAAAAG AGAACATCAC 
ATTGAACAAC TTAATGATAA GTTAAGCAAG ACAGAGAAAG AGTCCAAAGC CTTGCTGAGT 
GCTTTAGAGT TAAAAAAGAA AGAATATGAA GAATTGAAAG AAGAGAAAAC TCTGTTTTCT 
TGTTGGAAAA GTGAAAACGA AAAACTTTTA ACTCAGATGG AATCAGAAAA GGAAAACTTG 
CAGAGTAAAA TTAATCACTT GGAAACTTGT CTGAAGACAC AGCAAATAAA AAGTCATGAA 
TACAACGAGA GAGTAAGAAC GCTGGAGATG GACAGAGAAA ACCTAAGTGT CGAGATCAGA 
AACCTTCACA ACGTGTTAGA CAGTAAGTCA GTGGAGGTAG AGACCCAGAA ACTAGCTTAT 
ATGGAGCTAC AGCAGAAAGC TGAGTTCTCA GATCAGAAAC ATCAGAAGGA AATAGAAAAT 
ATGTGTTTGA AGACTTCTCA GCTTACTGGG CAAGTTGAAG ATCTAGAACA CAAGCTTCAG 
TTACTGTCAA ATGAAATAAT GGACAAAGAC CGGTGTTACC AAGACTTGCA TGCCGAATAT 
GAGAGCCTCA GGGATCTGCT AAAATCCAAA GATGCTTCTC TGGTGACAAA TGAAGATCAT 
CAGAGAAGTC TTTTGGCTTT TGATCAGCAG CCTGCCATGC ATCATTCCTT TGCAAATATA 
ATTGGAGAAC AAGGAAGCAT GCCTTCAGAG AGGAGTGAAT GTCGTTTAGA AGCAGACCAA 
AGTCCGAAAA ATTCTGCCAT CCTACAAAAT AGAGTTGATT CACTTGAATT TTCATTAGAG 
TCTCAAAAAC AGATGAACTC AGACCTGCAA AAGCAGTGTG AAGAGTTGGT GCAAATCAAA 
GGAGAAATAG AAGAAAATCT CATGAAAGCA GAACAGATGC ATCAAAGTTT TGTGGCTGAA 
ACAAGTCAGC GCATTAGTAA GTTACAGGAA GACACTTCTG CTCACCAGAA TGTTGTTGCT 
GAAACCTTAA GTGCCCTTGA GAACAAGGAA AAAGAGCTGC AACTTTTAAA TGATAAGGTA 
GAAACTGAGC AGGCAGAGAT TCAAGAATTA AAAAAGAGCA ACCATCTACT TGAAGACTCT 
CTAAAGGAGC TACAACTTTT ATCCGAAACC CTAAGCTTGG AGAAGAAAGA AATGAGTTCC 
ATCATTTCTC TAAATAAAAG GGAAATTGAA GAGCTGACCC AAGAGAATGG GACTCTTAAG 
GAAATTAATG CATCCTTAAA TCAAGAGAAG ATGAACTTAA TCCAGAAAAG TGAGAGTTTT 
GCAAACTATA TAGATGAAAG GGAGAAAAGC ATTTCAGAGT TATCTGATCA GTACAAGCAA 
GAAAAACTTA TTTTACTACA AAGATGTGAA GAAACCGGAA ATGCATATGA GGATCTTAGT 
CAAAAATACA AAGCAGCACA GGAAAAGAAT TCTAAATTAG AATGCTTGCT AAATGAATGC 
ACTAGTCTTT GTGAAAATAG GAAAAATGAG TTGGAACAGC TAAAGGAAGC ATTTGCAAAG 
GAACACCAAG AATTCTTAAC AAAATTAGCA TTTGCTGAAG AAAGAAATCA GAATCTGATG 
CTAGAGTTGG AGACAGTGCA GCAAGCTCTG AGATCTGAGA TGACAGATAA CCAAAACAAT 
TCTAAGAGCG AGGCTGGTGG TTTAAAGCAA GAAATCATGA CTTTAAAGGA AGAACAAAAC 
AAAATGCAAA AGGAAGTTAA TGACTTATTA CAAGAGAATG AACAGCTGAT GAAGGTAATG 
AAGACTAAAC ATGAATGTCA AAATCTAGAA TCAGAACCAA TTAGGAACTC TGTGAAAGAA 
AGAGAGAGTG AGAGAAATCA ATGTAATTTT AAACCTCAGA TGGATCTTGA AGTTAAAGAA 
ATTTCTCTAG ATAGTTATAA TGCGCAGTTG GTGCAATTAG AAGCTATGCT AAGAAATAAG 
GAATTAAAAC TTCAGGAAAG TGAGAAGGAG AAGGAGTGCC TGCAGCATGA ATTACAGACA 
ATTAGAGGAG ATCTTGAAAC CAGCAATTTG CAAGACATGC AGTCACAAGA AATTAGTGGC 
CTTAAAGACT GTGAAATAGA TGCGGAAGAA AAGTATATTT CAGGGCCTCA TGAGTTGTCA 
ACAAGTCAAA ACGACAATGC ACACCTTCAG TGCTCTCTGC AAACAACAAT GAACAAGCTG 
AATGAGCTAG AGAAAATATG TGAAATACTG CAGGCTGAAA AGTATGAACT CGTAACTGAG 
CTGAATGATT CAAGGTCAGA ATGTATCACA GCAACTAGGA AAATGGCAGA AGAGGTAGGG 
AAACTACTAA ATGAAGTTAA AATATTAAAT GATGACAGTG GTCTTCTCCA TGGTGAGTTA 
GTGGAAGACA TACCAGGAGG TGAATTTGGT GAACAACCAA ATGAACAGCA CCCTGTGTCT 
TTGGCTCCAT TGGACGAGAG TAATTCCTAC GAGCACTTGA CATTGTCAGA CAAAGAAGTT 
CAAATGCACT TTGCCGAATT GCAAGAGAAA TTCTTATCTT TACAAAGTGA ACACAAAATT 
TTACATGATC AGCACTGTCA GATGAGCTCT AAAATGTCAG AGCTGCAGAC CTATGTTGAC 
TCATTAAAGG CCGAAAATTT GGTCTTGTCA ACGAATCTGA GAAACTTTCA AGGTGACTTG 
GTGAAGGAGA TGCAGCTGGG CTTGGAGGAG GGGCTCGTTC CATCCCTGTC ATCCTCTTGT 
GTGCCTGACA GCTCTAGTCT TAGCAGTTTG GGAGACTCCT CCTTTTACAG AGCTCTTTTA 
GAACAGACAG GAGATATGTC TCTTTTGAGT AATTTAGAAG GGGCTGTTTC AGCAAACCAG 
TGCAGTGTAG ATGAAGTATT TTGCAGCAGT CTGCAGGAGG AGAATCTGAC CAGGAAAGAA 
ACCCCTTCGG CCCCAGCGAA GGGTGTTGAA GAGCTTGAGT CCCTCTGTGA GGTGTACCGG 
CAGTCCCTCG AGAAGCTAGA AGAGAAAATG GAAAGTCAAG GGATTATGAA AAATAAGGAA 
ATTCAAGAGC TCGAGCAGTT ATTAAGTTCT GAAAGGCAAG AGCTTGACTG CCTTAGGAAG 
CAGTATTTGT CAGAAAATGA ACAGTGGCAA CAGAAGCTGA CAAGCGTGAC TCTGGAGATG 
GAGTCCAAGT TGGCGGCAGA AAAGAAACAG ACGGAACAAC TGTCACTTGA GCTGGAAGTA 
GCACGACTCC AGCTACAAGG TCTGGACTTA AGTTCTCGGT CTTTGCTTGG CATCGACACA 
GAAGATGCTA TTCAAGGCCG AAATGAGAGC TGTGACATAT CAAAAGAACA TACTTCAGAA 
ACTACAGAAA GAACACCAAA GCATGATGTT CATCAGATTT GTGATAAAGA TGCTCAGCAG 
GACCTCAATC TAGACATTGA GAAAATAACT GAGACTGGTG CAGTGAAACC CACAGGAGAG 
TGCTCTGGGG AACAGTCCCC AGATACCAAT TATGAGCCTC CAGGGGAAGA TAAAACCCAG 
GGCTCTTCAG AATGCATTTC TGAATTGTCA TTTTCTGGTC CTAATGCTTT GGTACCTATG 
GATTTCCTGG GGAATCAGGA AGATATCCAT AATCTTCAAC TGCGGGTAAA AGAGACATCA 
AATGAGAATT TGAGATTACT TCATGTGATA GAGGACCGTG ACAGAAAAGT TGAAAGTTTG 
CTAAATGAAA TGAAAGAATT AGACTCAAAA CTCCATTTAC AGGAGGTACA ACTAATGACC 
AAAATTGAAG CATGCATAGA ATTGGAAAAA ATAGTTGGGG AACTTAAGAA AGAAAACTCA 
GATTTAAGTG AAAAATTGGA ATATTTTTCT TGTGATCACC AGGAGTTACT CCAGAGAGTA 
GAAACTTCTG AAGGCCTCAA TTCTGATTTA GAAATGCATG CAGATAAATC ATCACGTGAA 
GATATTGGAG ATAATGTGGC CAAGGTGAAT GACAGCTGGA AGGAGAGATT TCTTGATGTG 
GAAAATGAGC TGAGTAGGAT CAGATCGGAG AAAGCTAGCA TTGAGCATGA AGCCCTCTAC 
CTGGAGGCTG ACTTAGAGGT AGTTCAAACA GAGAAGCTAT GTTTAGAAAA AGACAATGAA 
AATAAGCAGA AGGTTATTGT CTGCCTTGAA GAAGAACTCT CAGTGGTCAC AAGTGAGAGA 
AACCAGCTTC GTGGAGAATT AGATACTATG TCAAAAAAAA CCACGGCACT GGATCAGTTG 
TCTGAAAAAA TGAAGGAGAA AACACAAGAG CTTGAGTCTC ATCAAAGTGA GTGTCTCCAT 
TGCATTCAGG TGGCAGAGGC AGAGGTGAAG GAAAAGACGG AACTCCTTCA GACTTTGTCC 
TCTGATGTGA GTGAGCTGTT AAAAGACAAA ACTCATCTCC AGGAAAAGCT GCAGAGTTTG 
GAAAAGGACT CACAGGCACT GTCTTTGACA AAATGTGAGC TGGAAAACCA AATTGCACAA 
CTGAATAAAG AGAAAGAATT GCTTGTCAAG GAATCTGAAA GCCTGCAGGC CAGACTGAGT 
GAATCAGATT ATGAAAAGCT GAATGTCTCC AAGGCCTTGG AGGCCGCACT GGTGGAGAAA 
GGTGAGTTCG CATTGAGGCT GAGCTCAACA CAGGAGGAAG TGCATCAGCT GAGAAGAGGC 
ATCGAGAAAC TGAGAGTTCG CATTGAGGCC GATGAAAAGA AGCAGCTGCA CATCGCAGAG 
AAACTGAAAG AACGCGAGCG GGAGAATGAT TCACTTAAGG ATAAAGTTGA GAACCTTGAA 
AGGGAATTGC AGATGTCAGA AGAAAACCAG GAGCTAGTGA TTCTTGATGC CGAGAATTCC 
AAAGCAGAAG TAGAGACTCT AAAAACACAA ATAGAAGAGA TGGCCAGAAG CCTGAAAGTT 
TTTGAATTAG ACCTTGTCAC GTTAAGGTCT GAAAAAGAAA ATCTGACAAA ACAAATACAA 
GAAAAACAAG GTCAGTTGTC AGAACTAGAC AAGTTACTCT CTTCATTTAA AAGTCTGTTA 
GAAGAAAAGG AGCAAGCAGA GATACAGATC AAAGAAGAAT CTAAAACTGC AGTGGAGATG 
CTTCAGAATC AGTTAAAGGA GCTAAATGAG GCAGTAGCAG CCTTGTGTGG TGACCAAGAA 
ATTATGAAGG CCACAGAACA GAGTCTAGAC CCACCAATAG AGGAAGAGCA TCAGCTGAGA 
AATAGCATTG AAAAGCTGAG AGCCCGCCTA GAAGCTGATG AAAAGAAGCA GCTCTGTGTC 
TTACAACAAC TGAAGGAAAG TGAGCATCAT GCAGATTTAC TTAAGGGTAG AGTGGAGAAC 
CTTGAAAGAG AGCTAGAGAT AGCCAGGACA AACCAAGAGC ATGCAGCTCT TGAGGCAGAG 
AATTCCAAAG GAGAGGTAGA GACCCTAAAA GCAAAAATAG AAGGGATGAC CCAAAGTCTG 
AGAGGTCTGG AATTAGATGT TGTTACTATA AGGTCAGAAA AAGAAAATCT GACAAATGAA 
TTACAAAAAG AGCAAGAGCG AATATCTGAA TTAGAAATAA TAAATTCATC ATTTGAAAAT 
ATTTTGCAAG AAAAAGAGCA AGAGAAAGTA CAGATGAAAG AAAAATCAAG CACTGCCATG 
GAGATGCTTC AAACACAATT AAAAGAGCTC AATGAGAGAG TGGCAGCCCT GCATAATGAC 
CAAGAAGCCT GTAAGGCCAA AGAGCAGAAT CTTAGTAGTC AAGTAGAGTG TCTTGAACTT 
GAGAAGGCTC AGTTGCTACA AGGCCTTGAT GAGGCCAAAA ATAATTATAT TGTTTTGCAA 
TCTTCAGTGA ATGGCCTCAT TCAAGAAGTA GAAGATGGCA AGCAGAAACT GGAGAAGAAG 
GATGAAGAAA TCAGTAGACT GAAAAATCAA ATTCAAGACC AAGAGCAGCT TGTCTCTAAA 
CTGTCCCAGG TGGAAGGAGA GCACCAACTT TGGAAGGAGC AAAACTTAGA ACTGAGAAAT 
CTGACAGTGG AATTGGAGCA GAAGATCCAA GTGCTACAAT CCAAAAATGC CTCTTTGCAG 
GACACATTAG AAGTGCTGCA GAGTTCTTAC AAGAATCTAG AGAATGAGCT TGAATTGACA 
AAAATGGACA AAATGTCCTT TGTTGAAAAA GTAAACAAAA TGACTGCAAA GGAAACTGAG 
CTGCAGAGGG AAATGCATGA GATGGCACAG AAAACAGCAG AGCTGCAAGA AGAACTCAGT 
GGAGAGAAAA ATAGGCTAGC TGGAGAGTTG CAGTTACTGT TGGAAGAAAT AAAGAGCAGC 
AAAGATCAAT TGAAGGAGCT CACACTAGAA AATAGTGAAT TGAAGAAGAG CCTAGATTGC 
ATGCACAAAG ACCAGGTGGA AAAGGAAGGG AAAGTGAGAG AGGAAATAGC TGAATATCAG 
CTACGGCTTC ATGAAGCTGA AAAGAAACAC CAGGCTTTGC TTTTGGACAC AAACAAACAG 
TATGAAGTAG AAATCCAGAC ATACCGAGAG AAATTGACTT CTAAAGAAGA ATGTCTCAGT 
TCACAGAAGC TGGAGATAGA CCTTTTAAAG TCTAGTAAAG AAGAGCTCAA TAATTCATTG 
AAAGCTACTA CTCAGATTTT GGAAGAATTG AAGAAAACCA AGATGGACAA TCTAAAATAT 
GTAAATCAGT TGAAGAAGGA AAATGAACGT GCCCAGGGGA AAATGAAGTT GTTGATCAAA 
TCCTGTAAAC AGCTGGAAGA GGAAAAGGAG ATACTGCAGA AAGAACTCTC TCAACTTCAA 
GCTGCACAGG AGAAGCAGAA AACAGGTACT GTTATGGATA CCAAGGTCGA TGAATTAACA 
ACTGAGATCA AAGAACTGAA AGAAACTCTT GAAGAAAAAA CCAAGGAGGC AGATGAATAC 
TTGGATAAGT ACTGTTCCTT GCTTATAAGC CATGAAAAGT TAGAGAAAGC TAAAGAGATG 
TTAGAGACAC AAGTGGCCCA TCTGTGTTCA CAGCAATCTA AACAAGATTC CCGAGGGTCT 
CCTTTGCTAG GTCCAGTTGT TCCAGGACCA TCTCCAATCC CTTCTGTTAC TGAAAAGAGG 
TTATCATCTG GCCAAAATAA AGCTTCAGGC AAGAGGCAAA GATCCAGTGG AATATGGGAG 
AATGGTAGAG GACCAACACC TGCTACCCCA GAGAGCTTTT CTAAAAAAAG CAAGAAAGCA 
GTCATGAGTG GTATTCACCC TGCAGAAGAC ACGGAAGGTA CTGAGTTTGA GCCAGAGGGA 
CTTCCAGAAG TTGTAAAGAA AGGGTTTGCT GACATCCCGA CAGGAAAGAC TAGCCCATAT 
ATCCTGCGAA GAACAACCAT GGCAACTCGG ACCAGCCCCC GCCTGGCTGC ACAGAAGTTA 
GCGCTATCCC CACTGAGTCT CGGCAAAGAA AATCTTGCAG AGTCCTCCAA ACCAACAGCT 
GGTGGCAGCA GATCACAAAA GGTCAAAGTT GCTCAGCGGA GCCCAGTAGA TTCAGGCACC 
ATCCTCCGAG AACCCACCAC GAAATCCGTC CCAGTCAATA ATCTTCCTGA GAGAAGTCCG 
ACTGACAGCC CCAGAGAGGG CCTGAGGGTC AAGCGAGGCC GACTTGTCCC CAGCCCCAAA 
GCTGGACTGG AGTCCAACGG CAGTGAGAAC TGTAAGGTCC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CENPF ( XM_017000086.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016855575.1 Homo sapiens centromere protein F (CENPF), transcript variant X1, mRNA.

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CloneID	OHu14151
Clone ID Related Accession (Same CDS sequence)	XM_017000086.1 , NM_016343.4 , NM_016343.3 , XM_017000086.2
Accession Version	XM_017000086.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9345bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	centromere protein F isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017000086.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTGGG CTTTGGAAGA ATGGAAAGAA GGGCTGCCTA CAAGAGCTCT TCAGAAAATT 
CAAGAGCTTG AAGGACAGCT TGACAAACTG AAGAAGGAAA AGCAGCAAAG GCAGTTTCAG 
CTTGACAGTC TCGAGGCTGC GCTGCAGAAG CAAAAACAGA AGGTTGAAAA TGAAAAAACC 
GAGGGTACAA ACCTGAAAAG GGAGAATCAA AGATTGATGG AAATATGTGA AAGTCTGGAG 
AAAACTAAGC AGAAGATTTC TCATGAACTT CAAGTCAAGG AGTCACAAGT GAATTTCCAG 
GAAGGACAAC TGAATTCAGG CAAAAAACAA ATAGAAAAAC TGGAACAGGA ACTTAAAAGG 
TGTAAATCTG AGCTTGAAAG AAGCCAACAA GCTGCGCAGT CTGCAGATGT CTCTCTGAAT 
CCATGCAATA CACCACAAAA AATTTTTACA ACTCCACTAA CACCAAGTCA ATATTATAGT 
GGTTCCAAGT ATGAAGATCT AAAAGAAAAA TATAATAAAG AGGTTGAAGA ACGAAAAAGA 
TTAGAGGCAG AGGTTAAAGC CTTGCAGGCT AAAAAAGCAA GCCAGACTCT TCCACAAGCC 
ACCATGAATC ACCGCGACAT TGCCCGGCAT CAGGCTTCAT CATCTGTGTT CTCATGGCAG 
CAAGAGAAGA CCCCAAGTCA TCTTTCATCT AATTCTCAAA GAACTCCAAT TAGGAGAGAT 
TTCTCTGCAT CTTACTTTTC TGGGGAACAA GAGGTGACTC CAAGTCGATC AACTTTGCAA 
ATAGGGAAAA GAGATGCTAA TAGCAGTTTC TTTGACAATT CTAGCAGTCC TCATCTTTTG 
GATCAATTAA AAGCGCAGAA TCAAGAGCTA AGAAACAAGA TTAATGAGTT GGAACTACGC 
CTGCAAGGAC ATGAAAAAGA AATGAAAGGC CAAGTGAATA AGTTTCAAGA ACTCCAACTC 
CAACTGGAGA AAGCAAAAGT GGAATTAATT GAAAAAGAGA AAGTTTTGAA CAAATGTAGG 
GATGAACTAG TGAGAACAAC AGCACAATAC GACCAGGCGT CAACCAAGTA TACTGCATTG 
GAACAAAAAC TGAAAAAATT GACGGAAGAT TTGAGTTGTC AGCGACAAAA TGCAGAAAGT 
GCCAGATGTT CTCTGGAACA GAAAATTAAG GAAAAAGAAA AGGAGTTTCA AGAGGAGCTC 
TCCCGTCAAC AGCGTTCTTT CCAAACACTG GACCAGGAGT GCATCCAGAT GAAGGCCAGA 
CTCACCCAGG AGTTACAGCA AGCCAAGAAT ATGCACAACG TCCTGCAGGC TGAACTGGAT 
AAACTCACAT CAGTAAAGCA ACAGCTAGAA AACAATTTGG AAGAGTTTAA GCAAAAGTTG 
TGCAGAGCTG AACAGGCGTT CCAGGCGAGT CAGATCAAGG AGAATGAGCT GAGGAGAAGC 
ATGGAGGAAA TGAAGAAGGA AAACAACCTC CTTAAGAGTC ACTCTGAGCA AAAGGCCAGA 
GAAGTCTGCC ACCTGGAGGC AGAACTCAAG AACATCAAAC AGTGTTTAAA TCAGAGCCAG 
AATTTTGCAG AAGAAATGAA AGCGAAGAAT ACCTCTCAGG AAACCATGTT AAGAGATCTT 
CAAGAAAAAA TAAATCAGCA AGAAAACTCC TTGACTTTAG AAAAACTGAA GCTTGCTGTG 
GCTGATCTGG AAAAGCAGCG AGATTGTTCT CAAGACCTTT TGAAGAAAAG AGAACATCAC 
ATTGAACAAC TTAATGATAA GTTAAGCAAG ACAGAGAAAG AGTCCAAAGC CTTGCTGAGT 
GCTTTAGAGT TAAAAAAGAA AGAATATGAA GAATTGAAAG AAGAGAAAAC TCTGTTTTCT 
TGTTGGAAAA GTGAAAACGA AAAACTTTTA ACTCAGATGG AATCAGAAAA GGAAAACTTG 
CAGAGTAAAA TTAATCACTT GGAAACTTGT CTGAAGACAC AGCAAATAAA AAGTCATGAA 
TACAACGAGA GAGTAAGAAC GCTGGAGATG GACAGAGAAA ACCTAAGTGT CGAGATCAGA 
AACCTTCACA ACGTGTTAGA CAGTAAGTCA GTGGAGGTAG AGACCCAGAA ACTAGCTTAT 
ATGGAGCTAC AGCAGAAAGC TGAGTTCTCA GATCAGAAAC ATCAGAAGGA AATAGAAAAT 
ATGTGTTTGA AGACTTCTCA GCTTACTGGG CAAGTTGAAG ATCTAGAACA CAAGCTTCAG 
TTACTGTCAA ATGAAATAAT GGACAAAGAC CGGTGTTACC AAGACTTGCA TGCCGAATAT 
GAGAGCCTCA GGGATCTGCT AAAATCCAAA GATGCTTCTC TGGTGACAAA TGAAGATCAT 
CAGAGAAGTC TTTTGGCTTT TGATCAGCAG CCTGCCATGC ATCATTCCTT TGCAAATATA 
ATTGGAGAAC AAGGAAGCAT GCCTTCAGAG AGGAGTGAAT GTCGTTTAGA AGCAGACCAA 
AGTCCGAAAA ATTCTGCCAT CCTACAAAAT AGAGTTGATT CACTTGAATT TTCATTAGAG 
TCTCAAAAAC AGATGAACTC AGACCTGCAA AAGCAGTGTG AAGAGTTGGT GCAAATCAAA 
GGAGAAATAG AAGAAAATCT CATGAAAGCA GAACAGATGC ATCAAAGTTT TGTGGCTGAA 
ACAAGTCAGC GCATTAGTAA GTTACAGGAA GACACTTCTG CTCACCAGAA TGTTGTTGCT 
GAAACCTTAA GTGCCCTTGA GAACAAGGAA AAAGAGCTGC AACTTTTAAA TGATAAGGTA 
GAAACTGAGC AGGCAGAGAT TCAAGAATTA AAAAAGAGCA ACCATCTACT TGAAGACTCT 
CTAAAGGAGC TACAACTTTT ATCCGAAACC CTAAGCTTGG AGAAGAAAGA AATGAGTTCC 
ATCATTTCTC TAAATAAAAG GGAAATTGAA GAGCTGACCC AAGAGAATGG GACTCTTAAG 
GAAATTAATG CATCCTTAAA TCAAGAGAAG ATGAACTTAA TCCAGAAAAG TGAGAGTTTT 
GCAAACTATA TAGATGAAAG GGAGAAAAGC ATTTCAGAGT TATCTGATCA GTACAAGCAA 
GAAAAACTTA TTTTACTACA AAGATGTGAA GAAACCGGAA ATGCATATGA GGATCTTAGT 
CAAAAATACA AAGCAGCACA GGAAAAGAAT TCTAAATTAG AATGCTTGCT AAATGAATGC 
ACTAGTCTTT GTGAAAATAG GAAAAATGAG TTGGAACAGC TAAAGGAAGC ATTTGCAAAG 
GAACACCAAG AATTCTTAAC AAAATTAGCA TTTGCTGAAG AAAGAAATCA GAATCTGATG 
CTAGAGTTGG AGACAGTGCA GCAAGCTCTG AGATCTGAGA TGACAGATAA CCAAAACAAT 
TCTAAGAGCG AGGCTGGTGG TTTAAAGCAA GAAATCATGA CTTTAAAGGA AGAACAAAAC 
AAAATGCAAA AGGAAGTTAA TGACTTATTA CAAGAGAATG AACAGCTGAT GAAGGTAATG 
AAGACTAAAC ATGAATGTCA AAATCTAGAA TCAGAACCAA TTAGGAACTC TGTGAAAGAA 
AGAGAGAGTG AGAGAAATCA ATGTAATTTT AAACCTCAGA TGGATCTTGA AGTTAAAGAA 
ATTTCTCTAG ATAGTTATAA TGCGCAGTTG GTGCAATTAG AAGCTATGCT AAGAAATAAG 
GAATTAAAAC TTCAGGAAAG TGAGAAGGAG AAGGAGTGCC TGCAGCATGA ATTACAGACA 
ATTAGAGGAG ATCTTGAAAC CAGCAATTTG CAAGACATGC AGTCACAAGA AATTAGTGGC 
CTTAAAGACT GTGAAATAGA TGCGGAAGAA AAGTATATTT CAGGGCCTCA TGAGTTGTCA 
ACAAGTCAAA ACGACAATGC ACACCTTCAG TGCTCTCTGC AAACAACAAT GAACAAGCTG 
AATGAGCTAG AGAAAATATG TGAAATACTG CAGGCTGAAA AGTATGAACT CGTAACTGAG 
CTGAATGATT CAAGGTCAGA ATGTATCACA GCAACTAGGA AAATGGCAGA AGAGGTAGGG 
AAACTACTAA ATGAAGTTAA AATATTAAAT GATGACAGTG GTCTTCTCCA TGGTGAGTTA 
GTGGAAGACA TACCAGGAGG TGAATTTGGT GAACAACCAA ATGAACAGCA CCCTGTGTCT 
TTGGCTCCAT TGGACGAGAG TAATTCCTAC GAGCACTTGA CATTGTCAGA CAAAGAAGTT 
CAAATGCACT TTGCCGAATT GCAAGAGAAA TTCTTATCTT TACAAAGTGA ACACAAAATT 
TTACATGATC AGCACTGTCA GATGAGCTCT AAAATGTCAG AGCTGCAGAC CTATGTTGAC 
TCATTAAAGG CCGAAAATTT GGTCTTGTCA ACGAATCTGA GAAACTTTCA AGGTGACTTG 
GTGAAGGAGA TGCAGCTGGG CTTGGAGGAG GGGCTCGTTC CATCCCTGTC ATCCTCTTGT 
GTGCCTGACA GCTCTAGTCT TAGCAGTTTG GGAGACTCCT CCTTTTACAG AGCTCTTTTA 
GAACAGACAG GAGATATGTC TCTTTTGAGT AATTTAGAAG GGGCTGTTTC AGCAAACCAG 
TGCAGTGTAG ATGAAGTATT TTGCAGCAGT CTGCAGGAGG AGAATCTGAC CAGGAAAGAA 
ACCCCTTCGG CCCCAGCGAA GGGTGTTGAA GAGCTTGAGT CCCTCTGTGA GGTGTACCGG 
CAGTCCCTCG AGAAGCTAGA AGAGAAAATG GAAAGTCAAG GGATTATGAA AAATAAGGAA 
ATTCAAGAGC TCGAGCAGTT ATTAAGTTCT GAAAGGCAAG AGCTTGACTG CCTTAGGAAG 
CAGTATTTGT CAGAAAATGA ACAGTGGCAA CAGAAGCTGA CAAGCGTGAC TCTGGAGATG 
GAGTCCAAGT TGGCGGCAGA AAAGAAACAG ACGGAACAAC TGTCACTTGA GCTGGAAGTA 
GCACGACTCC AGCTACAAGG TCTGGACTTA AGTTCTCGGT CTTTGCTTGG CATCGACACA 
GAAGATGCTA TTCAAGGCCG AAATGAGAGC TGTGACATAT CAAAAGAACA TACTTCAGAA 
ACTACAGAAA GAACACCAAA GCATGATGTT CATCAGATTT GTGATAAAGA TGCTCAGCAG 
GACCTCAATC TAGACATTGA GAAAATAACT GAGACTGGTG CAGTGAAACC CACAGGAGAG 
TGCTCTGGGG AACAGTCCCC AGATACCAAT TATGAGCCTC CAGGGGAAGA TAAAACCCAG 
GGCTCTTCAG AATGCATTTC TGAATTGTCA TTTTCTGGTC CTAATGCTTT GGTACCTATG 
GATTTCCTGG GGAATCAGGA AGATATCCAT AATCTTCAAC TGCGGGTAAA AGAGACATCA 
AATGAGAATT TGAGATTACT TCATGTGATA GAGGACCGTG ACAGAAAAGT TGAAAGTTTG 
CTAAATGAAA TGAAAGAATT AGACTCAAAA CTCCATTTAC AGGAGGTACA ACTAATGACC 
AAAATTGAAG CATGCATAGA ATTGGAAAAA ATAGTTGGGG AACTTAAGAA AGAAAACTCA 
GATTTAAGTG AAAAATTGGA ATATTTTTCT TGTGATCACC AGGAGTTACT CCAGAGAGTA 
GAAACTTCTG AAGGCCTCAA TTCTGATTTA GAAATGCATG CAGATAAATC ATCACGTGAA 
GATATTGGAG ATAATGTGGC CAAGGTGAAT GACAGCTGGA AGGAGAGATT TCTTGATGTG 
GAAAATGAGC TGAGTAGGAT CAGATCGGAG AAAGCTAGCA TTGAGCATGA AGCCCTCTAC 
CTGGAGGCTG ACTTAGAGGT AGTTCAAACA GAGAAGCTAT GTTTAGAAAA AGACAATGAA 
AATAAGCAGA AGGTTATTGT CTGCCTTGAA GAAGAACTCT CAGTGGTCAC AAGTGAGAGA 
AACCAGCTTC GTGGAGAATT AGATACTATG TCAAAAAAAA CCACGGCACT GGATCAGTTG 
TCTGAAAAAA TGAAGGAGAA AACACAAGAG CTTGAGTCTC ATCAAAGTGA GTGTCTCCAT 
TGCATTCAGG TGGCAGAGGC AGAGGTGAAG GAAAAGACGG AACTCCTTCA GACTTTGTCC 
TCTGATGTGA GTGAGCTGTT AAAAGACAAA ACTCATCTCC AGGAAAAGCT GCAGAGTTTG 
GAAAAGGACT CACAGGCACT GTCTTTGACA AAATGTGAGC TGGAAAACCA AATTGCACAA 
CTGAATAAAG AGAAAGAATT GCTTGTCAAG GAATCTGAAA GCCTGCAGGC CAGACTGAGT 
GAATCAGATT ATGAAAAGCT GAATGTCTCC AAGGCCTTGG AGGCCGCACT GGTGGAGAAA 
GGTGAGTTCG CATTGAGGCT GAGCTCAACA CAGGAGGAAG TGCATCAGCT GAGAAGAGGC 
ATCGAGAAAC TGAGAGTTCG CATTGAGGCC GATGAAAAGA AGCAGCTGCA CATCGCAGAG 
AAACTGAAAG AACGCGAGCG GGAGAATGAT TCACTTAAGG ATAAAGTTGA GAACCTTGAA 
AGGGAATTGC AGATGTCAGA AGAAAACCAG GAGCTAGTGA TTCTTGATGC CGAGAATTCC 
AAAGCAGAAG TAGAGACTCT AAAAACACAA ATAGAAGAGA TGGCCAGAAG CCTGAAAGTT 
TTTGAATTAG ACCTTGTCAC GTTAAGGTCT GAAAAAGAAA ATCTGACAAA ACAAATACAA 
GAAAAACAAG GTCAGTTGTC AGAACTAGAC AAGTTACTCT CTTCATTTAA AAGTCTGTTA 
GAAGAAAAGG AGCAAGCAGA GATACAGATC AAAGAAGAAT CTAAAACTGC AGTGGAGATG 
CTTCAGAATC AGTTAAAGGA GCTAAATGAG GCAGTAGCAG CCTTGTGTGG TGACCAAGAA 
ATTATGAAGG CCACAGAACA GAGTCTAGAC CCACCAATAG AGGAAGAGCA TCAGCTGAGA 
AATAGCATTG AAAAGCTGAG AGCCCGCCTA GAAGCTGATG AAAAGAAGCA GCTCTGTGTC 
TTACAACAAC TGAAGGAAAG TGAGCATCAT GCAGATTTAC TTAAGGGTAG AGTGGAGAAC 
CTTGAAAGAG AGCTAGAGAT AGCCAGGACA AACCAAGAGC ATGCAGCTCT TGAGGCAGAG 
AATTCCAAAG GAGAGGTAGA GACCCTAAAA GCAAAAATAG AAGGGATGAC CCAAAGTCTG 
AGAGGTCTGG AATTAGATGT TGTTACTATA AGGTCAGAAA AAGAAAATCT GACAAATGAA 
TTACAAAAAG AGCAAGAGCG AATATCTGAA TTAGAAATAA TAAATTCATC ATTTGAAAAT 
ATTTTGCAAG AAAAAGAGCA AGAGAAAGTA CAGATGAAAG AAAAATCAAG CACTGCCATG 
GAGATGCTTC AAACACAATT AAAAGAGCTC AATGAGAGAG TGGCAGCCCT GCATAATGAC 
CAAGAAGCCT GTAAGGCCAA AGAGCAGAAT CTTAGTAGTC AAGTAGAGTG TCTTGAACTT 
GAGAAGGCTC AGTTGCTACA AGGCCTTGAT GAGGCCAAAA ATAATTATAT TGTTTTGCAA 
TCTTCAGTGA ATGGCCTCAT TCAAGAAGTA GAAGATGGCA AGCAGAAACT GGAGAAGAAG 
GATGAAGAAA TCAGTAGACT GAAAAATCAA ATTCAAGACC AAGAGCAGCT TGTCTCTAAA 
CTGTCCCAGG TGGAAGGAGA GCACCAACTT TGGAAGGAGC AAAACTTAGA ACTGAGAAAT 
CTGACAGTGG AATTGGAGCA GAAGATCCAA GTGCTACAAT CCAAAAATGC CTCTTTGCAG 
GACACATTAG AAGTGCTGCA GAGTTCTTAC AAGAATCTAG AGAATGAGCT TGAATTGACA 
AAAATGGACA AAATGTCCTT TGTTGAAAAA GTAAACAAAA TGACTGCAAA GGAAACTGAG 
CTGCAGAGGG AAATGCATGA GATGGCACAG AAAACAGCAG AGCTGCAAGA AGAACTCAGT 
GGAGAGAAAA ATAGGCTAGC TGGAGAGTTG CAGTTACTGT TGGAAGAAAT AAAGAGCAGC 
AAAGATCAAT TGAAGGAGCT CACACTAGAA AATAGTGAAT TGAAGAAGAG CCTAGATTGC 
ATGCACAAAG ACCAGGTGGA AAAGGAAGGG AAAGTGAGAG AGGAAATAGC TGAATATCAG 
CTACGGCTTC ATGAAGCTGA AAAGAAACAC CAGGCTTTGC TTTTGGACAC AAACAAACAG 
TATGAAGTAG AAATCCAGAC ATACCGAGAG AAATTGACTT CTAAAGAAGA ATGTCTCAGT 
TCACAGAAGC TGGAGATAGA CCTTTTAAAG TCTAGTAAAG AAGAGCTCAA TAATTCATTG 
AAAGCTACTA CTCAGATTTT GGAAGAATTG AAGAAAACCA AGATGGACAA TCTAAAATAT 
GTAAATCAGT TGAAGAAGGA AAATGAACGT GCCCAGGGGA AAATGAAGTT GTTGATCAAA 
TCCTGTAAAC AGCTGGAAGA GGAAAAGGAG ATACTGCAGA AAGAACTCTC TCAACTTCAA 
GCTGCACAGG AGAAGCAGAA AACAGGTACT GTTATGGATA CCAAGGTCGA TGAATTAACA 
ACTGAGATCA AAGAACTGAA AGAAACTCTT GAAGAAAAAA CCAAGGAGGC AGATGAATAC 
TTGGATAAGT ACTGTTCCTT GCTTATAAGC CATGAAAAGT TAGAGAAAGC TAAAGAGATG 
TTAGAGACAC AAGTGGCCCA TCTGTGTTCA CAGCAATCTA AACAAGATTC CCGAGGGTCT 
CCTTTGCTAG GTCCAGTTGT TCCAGGACCA TCTCCAATCC CTTCTGTTAC TGAAAAGAGG 
TTATCATCTG GCCAAAATAA AGCTTCAGGC AAGAGGCAAA GATCCAGTGG AATATGGGAG 
AATGGTAGAG GACCAACACC TGCTACCCCA GAGAGCTTTT CTAAAAAAAG CAAGAAAGCA 
GTCATGAGTG GTATTCACCC TGCAGAAGAC ACGGAAGGTA CTGAGTTTGA GCCAGAGGGA 
CTTCCAGAAG TTGTAAAGAA AGGGTTTGCT GACATCCCGA CAGGAAAGAC TAGCCCATAT 
ATCCTGCGAA GAACAACCAT GGCAACTCGG ACCAGCCCCC GCCTGGCTGC ACAGAAGTTA 
GCGCTATCCC CACTGAGTCT CGGCAAAGAA AATCTTGCAG AGTCCTCCAA ACCAACAGCT 
GGTGGCAGCA GATCACAAAA GGTCAAAGTT GCTCAGCGGA GCCCAGTAGA TTCAGGCACC 
ATCCTCCGAG AACCCACCAC GAAATCCGTC CCAGTCAATA ATCTTCCTGA GAGAAGTCCG 
ACTGACAGCC CCAGAGAGGG CCTGAGGGTC AAGCGAGGCC GACTTGTCCC CAGCCCCAAA 
GCTGGACTGG AGTCCAACGG CAGTGAGAAC TGTAAGGTCC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016855575.1
CDS	162..9506
Translation	MSWALEEWKEGLPTRALQKIQELEGQLDKLKKEKQQRQFQLDSLEAALQKQKQKVENEKTEGTNLKRENQRLMEICESLEKTKQKISHELQVKESQVNFQEGQLNSGKKQIEKLEQELKRCKSELERSQQAAQSADVSLNPCNTPQKIFTTPLTPSQYYSGSKYEDLKEKYNKEVEERKRLEAEVKALQAKKASQTLPQATMNHRDIARHQASSSVFSWQQEKTPSHLSSNSQRTPIRRDFSASYFSGEQEVTPSRSTLQIGKRDANSSFFDNSSSPHLLDQLKAQNQELRNKINELELRLQGHEKEMKGQVNKFQELQLQLEKAKVELIEKEKVLNKCRDELVRTTAQYDQASTKYTALEQKLKKLTEDLSCQRQNAESARCSLEQKIKEKEKEFQEELSRQQRSFQTLDQECIQMKARLTQELQQAKNMHNVLQAELDKLTSVKQQLENNLEEFKQKLCRAEQAFQASQIKENELRRSMEEMKKENNLLKSHSEQKAREVCHLEAELKNIKQCLNQSQNFAEEMKAKNTSQETMLRDLQEKINQQENSLTLEKLKLAVADLEKQRDCSQDLLKKREHHIEQLNDKLSKTEKESKALLSALELKKKEYEELKEEKTLFSCWKSENEKLLTQMESEKENLQSKINHLETCLKTQQIKSHEYNERVRTLEMDRENLSVEIRNLHNVLDSKSVEVETQKLAYMELQQKAEFSDQKHQKEIENMCLKTSQLTGQVEDLEHKLQLLSNEIMDKDRCYQDLHAEYESLRDLLKSKDASLVTNEDHQRSLLAFDQQPAMHHSFANIIGEQGSMPSERSECRLEADQSPKNSAILQNRVDSLEFSLESQKQMNSDLQKQCEELVQIKGEIEENLMKAEQMHQSFVAETSQRISKLQEDTSAHQNVVAETLSALENKEKELQLLNDKVETEQAEIQELKKSNHLLEDSLKELQLLSETLSLEKKEMSSIISLNKREIEELTQENGTLKEINASLNQEKMNLIQKSESFANYIDEREKSISELSDQYKQEKLILLQRCEETGNAYEDLSQKYKAAQEKNSKLECLLNECTSLCENRKNELEQLKEAFAKEHQEFLTKLAFAEERNQNLMLELETVQQALRSEMTDNQNNSKSEAGGLKQEIMTLKEEQNKMQKEVNDLLQENEQLMKVMKTKHECQNLESEPIRNSVKERESERNQCNFKPQMDLEVKEISLDSYNAQLVQLEAMLRNKELKLQESEKEKECLQHELQTIRGDLETSNLQDMQSQEISGLKDCEIDAEEKYISGPHELSTSQNDNAHLQCSLQTTMNKLNELEKICEILQAEKYELVTELNDSRSECITATRKMAEEVGKLLNEVKILNDDSGLLHGELVEDIPGGEFGEQPNEQHPVSLAPLDESNSYEHLTLSDKEVQMHFAELQEKFLSLQSEHKILHDQHCQMSSKMSELQTYVDSLKAENLVLSTNLRNFQGDLVKEMQLGLEEGLVPSLSSSCVPDSSSLSSLGDSSFYRALLEQTGDMSLLSNLEGAVSANQCSVDEVFCSSLQEENLTRKETPSAPAKGVEELESLCEVYRQSLEKLEEKMESQGIMKNKEIQELEQLLSSERQELDCLRKQYLSENEQWQQKLTSVTLEMESKLAAEKKQTEQLSLELEVARLQLQGLDLSSRSLLGIDTEDAIQGRNESCDISKEHTSETTERTPKHDVHQICDKDAQQDLNLDIEKITETGAVKPTGECSGEQSPDTNYEPPGEDKTQGSSECISELSFSGPNALVPMDFLGNQEDIHNLQLRVKETSNENLRLLHVIEDRDRKVESLLNEMKELDSKLHLQEVQLMTKIEACIELEKIVGELKKENSDLSEKLEYFSCDHQELLQRVETSEGLNSDLEMHADKSSREDIGDNVAKVNDSWKERFLDVENELSRIRSEKASIEHEALYLEADLEVVQTEKLCLEKDNENKQKVIVCLEEELSVVTSERNQLRGELDTMSKKTTALDQLSEKMKEKTQELESHQSECLHCIQVAEAEVKEKTELLQTLSSDVSELLKDKTHLQEKLQSLEKDSQALSLTKCELENQIAQLNKEKELLVKESESLQARLSESDYEKLNVSKALEAALVEKGEFALRLSSTQEEVHQLRRGIEKLRVRIEADEKKQLHIAEKLKERERENDSLKDKVENLERELQMSEENQELVILDAENSKAEVETLKTQIEEMARSLKVFELDLVTLRSEKENLTKQIQEKQGQLSELDKLLSSFKSLLEEKEQAEIQIKEESKTAVEMLQNQLKELNEAVAALCGDQEIMKATEQSLDPPIEEEHQLRNSIEKLRARLEADEKKQLCVLQQLKESEHHADLLKGRVENLERELEIARTNQEHAALEAENSKGEVETLKAKIEGMTQSLRGLELDVVTIRSEKENLTNELQKEQERISELEIINSSFENILQEKEQEKVQMKEKSSTAMEMLQTQLKELNERVAALHNDQEACKAKEQNLSSQVECLELEKAQLLQGLDEAKNNYIVLQSSVNGLIQEVEDGKQKLEKKDEEISRLKNQIQDQEQLVSKLSQVEGEHQLWKEQNLELRNLTVELEQKIQVLQSKNASLQDTLEVLQSSYKNLENELELTKMDKMSFVEKVNKMTAKETELQREMHEMAQKTAELQEELSGEKNRLAGELQLLLEEIKSSKDQLKELTLENSELKKSLDCMHKDQVEKEGKVREEIAEYQLRLHEAEKKHQALLLDTNKQYEVEIQTYREKLTSKEECLSSQKLEIDLLKSSKEELNNSLKATTQILEELKKTKMDNLKYVNQLKKENERAQGKMKLLIKSCKQLEEEKEILQKELSQLQAAQEKQKTGTVMDTKVDELTTEIKELKETLEEKTKEADEYLDKYCSLLISHEKLEKAKEMLETQVAHLCSQQSKQDSRGSPLLGPVVPGPSPIPSVTEKRLSSGQNKASGKRQRSSGIWENGRGPTPATPESFSKKSKKAVMSGIHPAEDTEGTEFEPEGLPEVVKKGFADIPTGKTSPYILRRTTMATRTSPRLAAQKLALSPLSLGKENLAESSKPTAGGSRSQKVKVAQRSPVDSGTILREPTTKSVPVNNLPERSPTDSPREGLRVKRGRLVPSPKAGLESNGSENCKVQ

Target ORF information:

RefSeq Version	XM_017000086.2
Organism	Homo sapiens(human)
Definition	Homo sapiens centromere protein F (CENPF), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017000086.2

ORF Insert Sequence:

ATGAGCTGGG CTTTGGAAGA ATGGAAAGAA GGGCTGCCTA CAAGAGCTCT TCAGAAAATT 
CAAGAGCTTG AAGGACAGCT TGACAAACTG AAGAAGGAAA AGCAGCAAAG GCAGTTTCAG 
CTTGACAGTC TCGAGGCTGC GCTGCAGAAG CAAAAACAGA AGGTTGAAAA TGAAAAAACC 
GAGGGTACAA ACCTGAAAAG GGAGAATCAA AGATTGATGG AAATATGTGA AAGTCTGGAG 
AAAACTAAGC AGAAGATTTC TCATGAACTT CAAGTCAAGG AGTCACAAGT GAATTTCCAG 
GAAGGACAAC TGAATTCAGG CAAAAAACAA ATAGAAAAAC TGGAACAGGA ACTTAAAAGG 
TGTAAATCTG AGCTTGAAAG AAGCCAACAA GCTGCGCAGT CTGCAGATGT CTCTCTGAAT 
CCATGCAATA CACCACAAAA AATTTTTACA ACTCCACTAA CACCAAGTCA ATATTATAGT 
GGTTCCAAGT ATGAAGATCT AAAAGAAAAA TATAATAAAG AGGTTGAAGA ACGAAAAAGA 
TTAGAGGCAG AGGTTAAAGC CTTGCAGGCT AAAAAAGCAA GCCAGACTCT TCCACAAGCC 
ACCATGAATC ACCGCGACAT TGCCCGGCAT CAGGCTTCAT CATCTGTGTT CTCATGGCAG 
CAAGAGAAGA CCCCAAGTCA TCTTTCATCT AATTCTCAAA GAACTCCAAT TAGGAGAGAT 
TTCTCTGCAT CTTACTTTTC TGGGGAACAA GAGGTGACTC CAAGTCGATC AACTTTGCAA 
ATAGGGAAAA GAGATGCTAA TAGCAGTTTC TTTGACAATT CTAGCAGTCC TCATCTTTTG 
GATCAATTAA AAGCGCAGAA TCAAGAGCTA AGAAACAAGA TTAATGAGTT GGAACTACGC 
CTGCAAGGAC ATGAAAAAGA AATGAAAGGC CAAGTGAATA AGTTTCAAGA ACTCCAACTC 
CAACTGGAGA AAGCAAAAGT GGAATTAATT GAAAAAGAGA AAGTTTTGAA CAAATGTAGG 
GATGAACTAG TGAGAACAAC AGCACAATAC GACCAGGCGT CAACCAAGTA TACTGCATTG 
GAACAAAAAC TGAAAAAATT GACGGAAGAT TTGAGTTGTC AGCGACAAAA TGCAGAAAGT 
GCCAGATGTT CTCTGGAACA GAAAATTAAG GAAAAAGAAA AGGAGTTTCA AGAGGAGCTC 
TCCCGTCAAC AGCGTTCTTT CCAAACACTG GACCAGGAGT GCATCCAGAT GAAGGCCAGA 
CTCACCCAGG AGTTACAGCA AGCCAAGAAT ATGCACAACG TCCTGCAGGC TGAACTGGAT 
AAACTCACAT CAGTAAAGCA ACAGCTAGAA AACAATTTGG AAGAGTTTAA GCAAAAGTTG 
TGCAGAGCTG AACAGGCGTT CCAGGCGAGT CAGATCAAGG AGAATGAGCT GAGGAGAAGC 
ATGGAGGAAA TGAAGAAGGA AAACAACCTC CTTAAGAGTC ACTCTGAGCA AAAGGCCAGA 
GAAGTCTGCC ACCTGGAGGC AGAACTCAAG AACATCAAAC AGTGTTTAAA TCAGAGCCAG 
AATTTTGCAG AAGAAATGAA AGCGAAGAAT ACCTCTCAGG AAACCATGTT AAGAGATCTT 
CAAGAAAAAA TAAATCAGCA AGAAAACTCC TTGACTTTAG AAAAACTGAA GCTTGCTGTG 
GCTGATCTGG AAAAGCAGCG AGATTGTTCT CAAGACCTTT TGAAGAAAAG AGAACATCAC 
ATTGAACAAC TTAATGATAA GTTAAGCAAG ACAGAGAAAG AGTCCAAAGC CTTGCTGAGT 
GCTTTAGAGT TAAAAAAGAA AGAATATGAA GAATTGAAAG AAGAGAAAAC TCTGTTTTCT 
TGTTGGAAAA GTGAAAACGA AAAACTTTTA ACTCAGATGG AATCAGAAAA GGAAAACTTG 
CAGAGTAAAA TTAATCACTT GGAAACTTGT CTGAAGACAC AGCAAATAAA AAGTCATGAA 
TACAACGAGA GAGTAAGAAC GCTGGAGATG GACAGAGAAA ACCTAAGTGT CGAGATCAGA 
AACCTTCACA ACGTGTTAGA CAGTAAGTCA GTGGAGGTAG AGACCCAGAA ACTAGCTTAT 
ATGGAGCTAC AGCAGAAAGC TGAGTTCTCA GATCAGAAAC ATCAGAAGGA AATAGAAAAT 
ATGTGTTTGA AGACTTCTCA GCTTACTGGG CAAGTTGAAG ATCTAGAACA CAAGCTTCAG 
TTACTGTCAA ATGAAATAAT GGACAAAGAC CGGTGTTACC AAGACTTGCA TGCCGAATAT 
GAGAGCCTCA GGGATCTGCT AAAATCCAAA GATGCTTCTC TGGTGACAAA TGAAGATCAT 
CAGAGAAGTC TTTTGGCTTT TGATCAGCAG CCTGCCATGC ATCATTCCTT TGCAAATATA 
ATTGGAGAAC AAGGAAGCAT GCCTTCAGAG AGGAGTGAAT GTCGTTTAGA AGCAGACCAA 
AGTCCGAAAA ATTCTGCCAT CCTACAAAAT AGAGTTGATT CACTTGAATT TTCATTAGAG 
TCTCAAAAAC AGATGAACTC AGACCTGCAA AAGCAGTGTG AAGAGTTGGT GCAAATCAAA 
GGAGAAATAG AAGAAAATCT CATGAAAGCA GAACAGATGC ATCAAAGTTT TGTGGCTGAA 
ACAAGTCAGC GCATTAGTAA GTTACAGGAA GACACTTCTG CTCACCAGAA TGTTGTTGCT 
GAAACCTTAA GTGCCCTTGA GAACAAGGAA AAAGAGCTGC AACTTTTAAA TGATAAGGTA 
GAAACTGAGC AGGCAGAGAT TCAAGAATTA AAAAAGAGCA ACCATCTACT TGAAGACTCT 
CTAAAGGAGC TACAACTTTT ATCCGAAACC CTAAGCTTGG AGAAGAAAGA AATGAGTTCC 
ATCATTTCTC TAAATAAAAG GGAAATTGAA GAGCTGACCC AAGAGAATGG GACTCTTAAG 
GAAATTAATG CATCCTTAAA TCAAGAGAAG ATGAACTTAA TCCAGAAAAG TGAGAGTTTT 
GCAAACTATA TAGATGAAAG GGAGAAAAGC ATTTCAGAGT TATCTGATCA GTACAAGCAA 
GAAAAACTTA TTTTACTACA AAGATGTGAA GAAACCGGAA ATGCATATGA GGATCTTAGT 
CAAAAATACA AAGCAGCACA GGAAAAGAAT TCTAAATTAG AATGCTTGCT AAATGAATGC 
ACTAGTCTTT GTGAAAATAG GAAAAATGAG TTGGAACAGC TAAAGGAAGC ATTTGCAAAG 
GAACACCAAG AATTCTTAAC AAAATTAGCA TTTGCTGAAG AAAGAAATCA GAATCTGATG 
CTAGAGTTGG AGACAGTGCA GCAAGCTCTG AGATCTGAGA TGACAGATAA CCAAAACAAT 
TCTAAGAGCG AGGCTGGTGG TTTAAAGCAA GAAATCATGA CTTTAAAGGA AGAACAAAAC 
AAAATGCAAA AGGAAGTTAA TGACTTATTA CAAGAGAATG AACAGCTGAT GAAGGTAATG 
AAGACTAAAC ATGAATGTCA AAATCTAGAA TCAGAACCAA TTAGGAACTC TGTGAAAGAA 
AGAGAGAGTG AGAGAAATCA ATGTAATTTT AAACCTCAGA TGGATCTTGA AGTTAAAGAA 
ATTTCTCTAG ATAGTTATAA TGCGCAGTTG GTGCAATTAG AAGCTATGCT AAGAAATAAG 
GAATTAAAAC TTCAGGAAAG TGAGAAGGAG AAGGAGTGCC TGCAGCATGA ATTACAGACA 
ATTAGAGGAG ATCTTGAAAC CAGCAATTTG CAAGACATGC AGTCACAAGA AATTAGTGGC 
CTTAAAGACT GTGAAATAGA TGCGGAAGAA AAGTATATTT CAGGGCCTCA TGAGTTGTCA 
ACAAGTCAAA ACGACAATGC ACACCTTCAG TGCTCTCTGC AAACAACAAT GAACAAGCTG 
AATGAGCTAG AGAAAATATG TGAAATACTG CAGGCTGAAA AGTATGAACT CGTAACTGAG 
CTGAATGATT CAAGGTCAGA ATGTATCACA GCAACTAGGA AAATGGCAGA AGAGGTAGGG 
AAACTACTAA ATGAAGTTAA AATATTAAAT GATGACAGTG GTCTTCTCCA TGGTGAGTTA 
GTGGAAGACA TACCAGGAGG TGAATTTGGT GAACAACCAA ATGAACAGCA CCCTGTGTCT 
TTGGCTCCAT TGGACGAGAG TAATTCCTAC GAGCACTTGA CATTGTCAGA CAAAGAAGTT 
CAAATGCACT TTGCCGAATT GCAAGAGAAA TTCTTATCTT TACAAAGTGA ACACAAAATT 
TTACATGATC AGCACTGTCA GATGAGCTCT AAAATGTCAG AGCTGCAGAC CTATGTTGAC 
TCATTAAAGG CCGAAAATTT GGTCTTGTCA ACGAATCTGA GAAACTTTCA AGGTGACTTG 
GTGAAGGAGA TGCAGCTGGG CTTGGAGGAG GGGCTCGTTC CATCCCTGTC ATCCTCTTGT 
GTGCCTGACA GCTCTAGTCT TAGCAGTTTG GGAGACTCCT CCTTTTACAG AGCTCTTTTA 
GAACAGACAG GAGATATGTC TCTTTTGAGT AATTTAGAAG GGGCTGTTTC AGCAAACCAG 
TGCAGTGTAG ATGAAGTATT TTGCAGCAGT CTGCAGGAGG AGAATCTGAC CAGGAAAGAA 
ACCCCTTCGG CCCCAGCGAA GGGTGTTGAA GAGCTTGAGT CCCTCTGTGA GGTGTACCGG 
CAGTCCCTCG AGAAGCTAGA AGAGAAAATG GAAAGTCAAG GGATTATGAA AAATAAGGAA 
ATTCAAGAGC TCGAGCAGTT ATTAAGTTCT GAAAGGCAAG AGCTTGACTG CCTTAGGAAG 
CAGTATTTGT CAGAAAATGA ACAGTGGCAA CAGAAGCTGA CAAGCGTGAC TCTGGAGATG 
GAGTCCAAGT TGGCGGCAGA AAAGAAACAG ACGGAACAAC TGTCACTTGA GCTGGAAGTA 
GCACGACTCC AGCTACAAGG TCTGGACTTA AGTTCTCGGT CTTTGCTTGG CATCGACACA 
GAAGATGCTA TTCAAGGCCG AAATGAGAGC TGTGACATAT CAAAAGAACA TACTTCAGAA 
ACTACAGAAA GAACACCAAA GCATGATGTT CATCAGATTT GTGATAAAGA TGCTCAGCAG 
GACCTCAATC TAGACATTGA GAAAATAACT GAGACTGGTG CAGTGAAACC CACAGGAGAG 
TGCTCTGGGG AACAGTCCCC AGATACCAAT TATGAGCCTC CAGGGGAAGA TAAAACCCAG 
GGCTCTTCAG AATGCATTTC TGAATTGTCA TTTTCTGGTC CTAATGCTTT GGTACCTATG 
GATTTCCTGG GGAATCAGGA AGATATCCAT AATCTTCAAC TGCGGGTAAA AGAGACATCA 
AATGAGAATT TGAGATTACT TCATGTGATA GAGGACCGTG ACAGAAAAGT TGAAAGTTTG 
CTAAATGAAA TGAAAGAATT AGACTCAAAA CTCCATTTAC AGGAGGTACA ACTAATGACC 
AAAATTGAAG CATGCATAGA ATTGGAAAAA ATAGTTGGGG AACTTAAGAA AGAAAACTCA 
GATTTAAGTG AAAAATTGGA ATATTTTTCT TGTGATCACC AGGAGTTACT CCAGAGAGTA 
GAAACTTCTG AAGGCCTCAA TTCTGATTTA GAAATGCATG CAGATAAATC ATCACGTGAA 
GATATTGGAG ATAATGTGGC CAAGGTGAAT GACAGCTGGA AGGAGAGATT TCTTGATGTG 
GAAAATGAGC TGAGTAGGAT CAGATCGGAG AAAGCTAGCA TTGAGCATGA AGCCCTCTAC 
CTGGAGGCTG ACTTAGAGGT AGTTCAAACA GAGAAGCTAT GTTTAGAAAA AGACAATGAA 
AATAAGCAGA AGGTTATTGT CTGCCTTGAA GAAGAACTCT CAGTGGTCAC AAGTGAGAGA 
AACCAGCTTC GTGGAGAATT AGATACTATG TCAAAAAAAA CCACGGCACT GGATCAGTTG 
TCTGAAAAAA TGAAGGAGAA AACACAAGAG CTTGAGTCTC ATCAAAGTGA GTGTCTCCAT 
TGCATTCAGG TGGCAGAGGC AGAGGTGAAG GAAAAGACGG AACTCCTTCA GACTTTGTCC 
TCTGATGTGA GTGAGCTGTT AAAAGACAAA ACTCATCTCC AGGAAAAGCT GCAGAGTTTG 
GAAAAGGACT CACAGGCACT GTCTTTGACA AAATGTGAGC TGGAAAACCA AATTGCACAA 
CTGAATAAAG AGAAAGAATT GCTTGTCAAG GAATCTGAAA GCCTGCAGGC CAGACTGAGT 
GAATCAGATT ATGAAAAGCT GAATGTCTCC AAGGCCTTGG AGGCCGCACT GGTGGAGAAA 
GGTGAGTTCG CATTGAGGCT GAGCTCAACA CAGGAGGAAG TGCATCAGCT GAGAAGAGGC 
ATCGAGAAAC TGAGAGTTCG CATTGAGGCC GATGAAAAGA AGCAGCTGCA CATCGCAGAG 
AAACTGAAAG AACGCGAGCG GGAGAATGAT TCACTTAAGG ATAAAGTTGA GAACCTTGAA 
AGGGAATTGC AGATGTCAGA AGAAAACCAG GAGCTAGTGA TTCTTGATGC CGAGAATTCC 
AAAGCAGAAG TAGAGACTCT AAAAACACAA ATAGAAGAGA TGGCCAGAAG CCTGAAAGTT 
TTTGAATTAG ACCTTGTCAC GTTAAGGTCT GAAAAAGAAA ATCTGACAAA ACAAATACAA 
GAAAAACAAG GTCAGTTGTC AGAACTAGAC AAGTTACTCT CTTCATTTAA AAGTCTGTTA 
GAAGAAAAGG AGCAAGCAGA GATACAGATC AAAGAAGAAT CTAAAACTGC AGTGGAGATG 
CTTCAGAATC AGTTAAAGGA GCTAAATGAG GCAGTAGCAG CCTTGTGTGG TGACCAAGAA 
ATTATGAAGG CCACAGAACA GAGTCTAGAC CCACCAATAG AGGAAGAGCA TCAGCTGAGA 
AATAGCATTG AAAAGCTGAG AGCCCGCCTA GAAGCTGATG AAAAGAAGCA GCTCTGTGTC 
TTACAACAAC TGAAGGAAAG TGAGCATCAT GCAGATTTAC TTAAGGGTAG AGTGGAGAAC 
CTTGAAAGAG AGCTAGAGAT AGCCAGGACA AACCAAGAGC ATGCAGCTCT TGAGGCAGAG 
AATTCCAAAG GAGAGGTAGA GACCCTAAAA GCAAAAATAG AAGGGATGAC CCAAAGTCTG 
AGAGGTCTGG AATTAGATGT TGTTACTATA AGGTCAGAAA AAGAAAATCT GACAAATGAA 
TTACAAAAAG AGCAAGAGCG AATATCTGAA TTAGAAATAA TAAATTCATC ATTTGAAAAT 
ATTTTGCAAG AAAAAGAGCA AGAGAAAGTA CAGATGAAAG AAAAATCAAG CACTGCCATG 
GAGATGCTTC AAACACAATT AAAAGAGCTC AATGAGAGAG TGGCAGCCCT GCATAATGAC 
CAAGAAGCCT GTAAGGCCAA AGAGCAGAAT CTTAGTAGTC AAGTAGAGTG TCTTGAACTT 
GAGAAGGCTC AGTTGCTACA AGGCCTTGAT GAGGCCAAAA ATAATTATAT TGTTTTGCAA 
TCTTCAGTGA ATGGCCTCAT TCAAGAAGTA GAAGATGGCA AGCAGAAACT GGAGAAGAAG 
GATGAAGAAA TCAGTAGACT GAAAAATCAA ATTCAAGACC AAGAGCAGCT TGTCTCTAAA 
CTGTCCCAGG TGGAAGGAGA GCACCAACTT TGGAAGGAGC AAAACTTAGA ACTGAGAAAT 
CTGACAGTGG AATTGGAGCA GAAGATCCAA GTGCTACAAT CCAAAAATGC CTCTTTGCAG 
GACACATTAG AAGTGCTGCA GAGTTCTTAC AAGAATCTAG AGAATGAGCT TGAATTGACA 
AAAATGGACA AAATGTCCTT TGTTGAAAAA GTAAACAAAA TGACTGCAAA GGAAACTGAG 
CTGCAGAGGG AAATGCATGA GATGGCACAG AAAACAGCAG AGCTGCAAGA AGAACTCAGT 
GGAGAGAAAA ATAGGCTAGC TGGAGAGTTG CAGTTACTGT TGGAAGAAAT AAAGAGCAGC 
AAAGATCAAT TGAAGGAGCT CACACTAGAA AATAGTGAAT TGAAGAAGAG CCTAGATTGC 
ATGCACAAAG ACCAGGTGGA AAAGGAAGGG AAAGTGAGAG AGGAAATAGC TGAATATCAG 
CTACGGCTTC ATGAAGCTGA AAAGAAACAC CAGGCTTTGC TTTTGGACAC AAACAAACAG 
TATGAAGTAG AAATCCAGAC ATACCGAGAG AAATTGACTT CTAAAGAAGA ATGTCTCAGT 
TCACAGAAGC TGGAGATAGA CCTTTTAAAG TCTAGTAAAG AAGAGCTCAA TAATTCATTG 
AAAGCTACTA CTCAGATTTT GGAAGAATTG AAGAAAACCA AGATGGACAA TCTAAAATAT 
GTAAATCAGT TGAAGAAGGA AAATGAACGT GCCCAGGGGA AAATGAAGTT GTTGATCAAA 
TCCTGTAAAC AGCTGGAAGA GGAAAAGGAG ATACTGCAGA AAGAACTCTC TCAACTTCAA 
GCTGCACAGG AGAAGCAGAA AACAGGTACT GTTATGGATA CCAAGGTCGA TGAATTAACA 
ACTGAGATCA AAGAACTGAA AGAAACTCTT GAAGAAAAAA CCAAGGAGGC AGATGAATAC 
TTGGATAAGT ACTGTTCCTT GCTTATAAGC CATGAAAAGT TAGAGAAAGC TAAAGAGATG 
TTAGAGACAC AAGTGGCCCA TCTGTGTTCA CAGCAATCTA AACAAGATTC CCGAGGGTCT 
CCTTTGCTAG GTCCAGTTGT TCCAGGACCA TCTCCAATCC CTTCTGTTAC TGAAAAGAGG 
TTATCATCTG GCCAAAATAA AGCTTCAGGC AAGAGGCAAA GATCCAGTGG AATATGGGAG 
AATGGTAGAG GACCAACACC TGCTACCCCA GAGAGCTTTT CTAAAAAAAG CAAGAAAGCA 
GTCATGAGTG GTATTCACCC TGCAGAAGAC ACGGAAGGTA CTGAGTTTGA GCCAGAGGGA 
CTTCCAGAAG TTGTAAAGAA AGGGTTTGCT GACATCCCGA CAGGAAAGAC TAGCCCATAT 
ATCCTGCGAA GAACAACCAT GGCAACTCGG ACCAGCCCCC GCCTGGCTGC ACAGAAGTTA 
GCGCTATCCC CACTGAGTCT CGGCAAAGAA AATCTTGCAG AGTCCTCCAA ACCAACAGCT 
GGTGGCAGCA GATCACAAAA GGTCAAAGTT GCTCAGCGGA GCCCAGTAGA TTCAGGCACC 
ATCCTCCGAG AACCCACCAC GAAATCCGTC CCAGTCAATA ATCTTCCTGA GAGAAGTCCG 
ACTGACAGCC CCAGAGAGGG CCTGAGGGTC AAGCGAGGCC GACTTGTCCC CAGCCCCAAA 
GCTGGACTGG AGTCCAACGG CAGTGAGAAC TGTAAGGTCC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CENPF ( XM_011509082.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011507384.1 Homo sapiens centromere protein F (CENPF), transcript variant X2, mRNA.

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CloneID	OHu56821
Clone ID Related Accession (Same CDS sequence)	XM_011509082.3 , XM_011509082.2
Accession Version	XM_011509082.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9168bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	centromere protein F isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011509082.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTGGG CTTTGGAAGA ATGGAAAGAA GGGCTGCCTA CAAGAGCTCT TCAGAAAATT 
CAAGAGCTTG AAGGACAGCT TGACAAACTG AAGAAGGAAA AGCAGCAAAG GCAGTTTCAG 
CTTGACAGTC TCGAGGCTGC GCTGCAGAAG CAAAAACAGA AGGTTGAAAA TGAAAAAACC 
GAGGGTACAA ACCTGAAAAG GGAGAATCAA AGATTGATGG AAATATGTGA AAGTCTGGAG 
AAAACTAAGC AGAAGATTTC TCATGAACTT CAAGTCAAGG AGTCACAAGT GAATTTCCAG 
GAAGGACAAC TGAATTCAGG CAAAAAACAA ATAGAAAAAC TGGAACAGGA ACTTAAAAGG 
TGTAAATCTG AGCTTGAAAG AAGCCAACAA GCTGCGCAGT CTGCAGATGT CTCTCTGAAT 
CCATGCAATA CACCACAAAA AATTTTTACA ACTCCACTAA CACCAAGTCA ATATTATAGT 
GGTTCCAAGT ATGAAGATCT AAAAGAAAAA TATAATAAAG AGGTTGAAGA ACGAAAAAGA 
TTAGAGGCAG AGGTTAAAGC CTTGCAGGCT AAAAAAGCAA GCCAGACTCT TCCACAAGCC 
ACCATGAATC ACCGCGACAT TGCCCGGCAT CAGGCTTCAT CATCTGTGTT CTCATGGCAG 
CAAGAGAAGA CCCCAAGTCA TCTTTCATCT AATTCTCAAA GAACTCCAAT TAGGAGAGAT 
TTCTCTGCAT CTTACTTTTC TGGGGAACAA GAGGTGACTC CAAGTCGATC AACTTTGCAA 
ATAGGGAAAA GAGATGCTAA TAGCAGTTTC TTTGACAATT CTAGCAGTCC TCATCTTTTG 
GATCAATTAA AAGCGCAGAA TCAAGAGCTA AGAAACAAGA TTAATGAGTT GGAACTACGC 
CTGCAAGGAC ATGAAAAAGA AATGAAAGGC CAAGTGAATA AGTTTCAAGA ACTCCAACTC 
CAACTGGAGA AAGCAAAAGT GGAATTAATT GAAAAAGAGA AAGTTTTGAA CAAATGTAGG 
GATGAACTAG TGAGAACAAC AGCACAATAC GACCAGGCGT CAACCAAGTA TACTGCATTG 
GAACAAAAAC TGAAAAAATT GACGGAAGAT TTGAGTTGTC AGCGACAAAA TGCAGAAAGT 
GCCAGATGTT CTCTGGAACA GAAAATTAAG GAAAAAGAAA AGGAGTTTCA AGAGGAGCTC 
TCCCGTCAAC AGCGTTCTTT CCAAACACTG GACCAGGAGT GCATCCAGAT GAAGGCCAGA 
CTCACCCAGG AGTTACAGCA AGCCAAGAAT ATGCACAACG TCCTGCAGGC TGAACTGGAT 
AAACTCACAT CAGTAAAGCA ACAGCTAGAA AACAATTTGG AAGAGTTTAA GCAAAAGTTG 
TGCAGAGCTG AACAGGCGTT CCAGGCGAGT CAGATCAAGG AGAATGAGCT GAGGAGAAGC 
ATGGAGGAAA TGAAGAAGGA AAACAACCTC CTTAAGAGTC ACTCTGAGCA AAAGGCCAGA 
GAAGTCTGCC ACCTGGAGGC AGAACTCAAG AACATCAAAC AGTGTTTAAA TCAGAGCCAG 
AATTTTGCAG AAGAAATGAA AGCGAAGAAT ACCTCTCAGG AAACCATGTT AAGAGATCTT 
CAAGAAAAAA TAAATCAGCA AGAAAACTCC TTGACTTTAG AAAAACTGAA GCTTGCTGTG 
GCTGATCTGG AAAAGCAGCG AGATTGTTCT CAAGACCTTT TGAAGAAAAG AGAACATCAC 
ATTGAACAAC TTAATGATAA GTTAAGCAAG ACAGAGAAAG AGTCCAAAGC CTTGCTGAGT 
GCTTTAGAGT TAAAAAAGAA AGAATATGAA GAATTGAAAG AAGAGAAAAC TCTGTTTTCT 
TGTTGGAAAA GTGAAAACGA AAAACTTTTA ACTCAGATGG AATCAGAAAA GGAAAACTTG 
CAGAGTAAAA TTAATCACTT GGAAACTTGT CTGAAGACAC AGCAAATAAA AAGTCATGAA 
TACAACGAGA GAGTAAGAAC GCTGGAGATG GACAGAGAAA ACCTAAGTGT CGAGATCAGA 
AACCTTCACA ACGTGTTAGA CAGTAAGTCA GTGGAGGTAG AGACCCAGAA ACTAGCTTAT 
ATGGAGCTAC AGCAGAAAGC TGAGTTCTCA GATCAGAAAC ATCAGAAGGA AATAGAAAAT 
ATGTGTTTGA AGACTTCTCA GCTTACTGGG CAAGTTGAAG ATCTAGAACA CAAGCTTCAG 
TTACTGTCAA ATGAAATAAT GGACAAAGAC CGGTGTTACC AAGACTTGCA TGCCGAATAT 
GAGAGCCTCA GGGATCTGCT AAAATCCAAA GATGCTTCTC TGGTGACAAA TGAAGATCAT 
CAGAGAAGTC TTTTGGCTTT TGATCAGCAG CCTGCCATGC ATCATTCCTT TGCAAATATA 
ATTGGAGAAC AAGGAAGCAT GCCTTCAGAG AGGAGTGAAT GTCGTTTAGA AGCAGACCAA 
AGTCCGAAAA ATTCTGCCAT CCTACAAAAT AGAGTTGATT CACTTGAATT TTCATTAGAG 
TCTCAAAAAC AGATGAACTC AGACCTGCAA AAGCAGTGTG AAGAGTTGGT GCAAATCAAA 
GGAGAAATAG AAGAAAATCT CATGAAAGCA GAACAGATGC ATCAAAGTTT TGTGGCTGAA 
ACAAGTCAGC GCATTAGTAA GTTACAGGAA GACACTTCTG CTCACCAGAA TGTTGTTGCT 
GAAACCTTAA GTGCCCTTGA GAACAAGGAA AAAGAGCTGC AACTTTTAAA TGATAAGGTA 
GAAACTGAGC AGGCAGAGAT TCAAGAATTA AAAAAGAGCA ACCATCTACT TGAAGACTCT 
CTAAAGGAGC TACAACTTTT ATCCGAAACC CTAAGCTTGG AGAAGAAAGA AATGAGTTCC 
ATCATTTCTC TAAATAAAAG GGAAATTGAA GAGCTGACCC AAGAGAATGG GACTCTTAAG 
GAAATTAATG CATCCTTAAA TCAAGAGAAG ATGAACTTAA TCCAGAAAAG TGAGAGTTTT 
GCAAACTATA TAGATGAAAG GGAGAAAAGC ATTTCAGAGT TATCTGATCA GTACAAGCAA 
GAAAAACTTA TTTTACTACA AAGATGTGAA GAAACCGGAA ATGCATATGA GGATCTTAGT 
CAAAAATACA AAGCAGCACA GGAAAAGAAT TCTAAATTAG AATGCTTGCT AAATGAATGC 
ACTAGTCTTT GTGAAAATAG GAAAAATGAG TTGGAACAGC TAAAGGAAGC ATTTGCAAAG 
GAACACCAAG AATTCTTAAC AAAATTAGCA TTTGCTGAAG AAAGAAATCA GAATCTGATG 
CTAGAGTTGG AGACAGTGCA GCAAGCTCTG AGATCTGAGA TGACAGATAA CCAAAACAAT 
TCTAAGAGCG AGGCTGGTGG TTTAAAGCAA GAAATCATGA CTTTAAAGGA AGAACAAAAC 
AAAATGCAAA AGGAAGTTAA TGACTTATTA CAAGAGAATG AACAGCTGAT GAAGGTAATG 
AAGACTAAAC ATGAATGTCA AAATCTAGAA TCAGAACCAA TTAGGAACTC TGTGAAAGAA 
AGAGAGAGTG AGAGAAATCA ATGTAATTTT AAACCTCAGA TGGATCTTGA AGTTAAAGAA 
ATTTCTCTAG ATAGTTATAA TGCGCAGTTG GTGCAATTAG AAGCTATGCT AAGAAATAAG 
GAATTAAAAC TTCAGGAAAG TGAGAAGGAG AAGGAGTGCC TGCAGCATGA ATTACAGACA 
ATTAGAGGAG ATCTTGAAAC CAGCAATTTG CAAGACATGC AGTCACAAGA AATTAGTGGC 
CTTAAAGACT GTGAAATAGA TGCGGAAGAA AAGTATATTT CAGGGCCTCA TGAGTTGTCA 
ACAAGTCAAA ACGACAATGC ACACCTTCAG TGCTCTCTGC AAACAACAAT GAACAAGCTG 
AATGAGCTAG AGAAAATATG TGAAATACTG CAGGCTGAAA AGTATGAACT CGTAACTGAG 
CTGAATGATT CAAGGTCAGA ATGTATCACA GCAACTAGGA AAATGGCAGA AGAGGTAGGG 
AAACTACTAA ATGAAGTTAA AATATTAAAT GATGACAGTG GTCTTCTCCA TGGTGAGTTA 
GTGGAAGACA TACCAGGAGG TGAATTTGGT GAACAACCAA ATGAACAGCA CCCTGTGTCT 
TTGGCTCCAT TGGACGAGAG TAATTCCTAC GAGCACTTGA CATTGTCAGA CAAAGAAGTT 
CAAATGCACT TTGCCGAATT GCAAGAGAAA TTCTTATCTT TACAAAGTGA ACACAAAATT 
TTACATGATC AGCACTGTCA GATGAGCTCT AAAATGTCAG AGCTGCAGAC CTATGTTGAC 
TCATTAAAGG CCGAAAATTT GGTCTTGTCA ACGAATCTGA GAAACTTTCA AGGTGACTTG 
GTGAAGGAGA TGCAGCTGGG CTTGGAGGAG GGGCTCGTTC CATCCCTGTC ATCCTCTTGT 
GTGCCTGACA GCTCTAGTCT TAGCAGTTTG GGAGACTCCT CCTTTTACAG AGCTCTTTTA 
GAACAGACAG GAGATATGTC TCTTTTGAGT AATTTAGAAG GGGCTGTTTC AGCAAACCAG 
TGCAGTGTAG ATGAAGTATT TTGCAGCAGT CTGCAGGAGG AGAATCTGAC CAGGAAAGAA 
ACCCCTTCGG CCCCAGCGAA GGGTGTTGAA GAGCTTGAGT CCCTCTGTGA GGTGTACCGG 
CAGTCCCTCG AGAAGCTAGA AGAGAAAATG GAAAGTCAAG GGATTATGAA AAATAAGGAA 
ATTCAAGAGC TCGAGCAGTT ATTAAGTTCT GAAAGGCAAG AGCTTGACTG CCTTAGGAAG 
CAGTATTTGT CAGAAAATGA ACAGTGGCAA CAGAAGCTGA CAAGCGTGAC TCTGGAGATG 
GAGTCCAAGT TGGCGGCAGA AAAGAAACAG ACGGAACAAC TGTCACTTGA GCTGGAAGTA 
GCACGACTCC AGCTACAAGG TCTGGACTTA AGTTCTCGGT CTTTGCTTGG CATCGACACA 
GAAGATGCTA TTCAAGGCCG AAATGAGAGC TGTGACATAT CAAAAGAACA TACTTCAGAA 
ACTACAGAAA GAACACCAAA GCATGATGTT CATCAGATTT GTGATAAAGA TGCTCAGCAG 
GACCTCAATC TAGACATTGA GAAAATAACT GAGACTGGTG CAGTGAAACC CACAGGAGAG 
TGCTCTGGGG AACAGTCCCC AGATACCAAT TATGAGCCTC CAGGGGAAGA TAAAACCCAG 
GGCTCTTCAG AATGCATTTC TGAATTGTCA TTTTCTGGTC CTAATGCTTT GGTACCTATG 
GATTTCCTGG GGAATCAGGA AGATATCCAT AATCTTCAAC TGCGGGTAAA AGAGACATCA 
AATGAGAATT TGAGATTACT TCATGTGATA GAGGACCGTG ACAGAAAAGT TGAAAGTTTG 
CTAAATGAAA TGAAAGAATT AGACTCAAAA CTCCATTTAC AGGAGGTACA ACTAATGACC 
AAAATTGAAG CATGCATAGA ATTGGAAAAA ATAGTTGGGG AACTTAAGAA AGAAAACTCA 
GATTTAAGTG AAAAATTGGA ATATTTTTCT TGTGATCACC AGGAGTTACT CCAGAGAGTA 
GAAACTTCTG AAGGCCTCAA TTCTGATTTA GAAATGCATG CAGATAAATC ATCACGTGAA 
GATATTGGAG ATAATGTGGC CAAGGTGAAT GACAGCTGGA AGGAGAGATT TCTTGATGTG 
GAAAATGAGC TGAGTAGGAT CAGATCGGAG AAAGCTAGCA TTGAGCATGA AGCCCTCTAC 
CTGGAGGCTG ACTTAGAGGT AGTTCAAACA GAGAAGCTAT GTTTAGAAAA AGACAATGAA 
AATAAGCAGA AGGTTATTGT CTGCCTTGAA GAAGAACTCT CAGTGGTCAC AAGTGAGAGA 
AACCAGCTTC GTGGAGAATT AGATACTATG TCAAAAAAAA CCACGGCACT GGATCAGTTG 
TCTGAAAAAA TGAAGGAGAA AACACAAGAG CTTGAGTCTC ATCAAAGTGA GTGTCTCCAT 
TGCATTCAGG TGGCAGAGGC AGAGGTGAAG GAAAAGACGG AACTCCTTCA GACTTTGTCC 
TCTGATGTGA GTGAGCTGTT AAAAGACAAA ACTCATCTCC AGGAAAAGCT GCAGAGTTTG 
GAAAAGGACT CACAGGCACT GTCTTTGACA AAATGTGAGC TGGAAAACCA AATTGCACAA 
CTGAATAAAG AGAAAGAATT GCTTGTCAAG GAATCTGAAA GCCTGCAGGC CAGACTGAGT 
GAATCAGATT ATGAAAAGCT GAATGTCTCC AAGGCCTTGG AGGCCGCACT GGTGGAGAAA 
GGTGAGTTCG CATTGAGGCT GAGCTCAACA CAGGAGGAAG TGCATCAGCT GAGAAGAGGC 
ATCGAGAAAC TGAGAGTTCG CATTGAGGCC GATGAAAAGA AGCAGCTGCA CATCGCAGAG 
AAACTGAAAG AACGCGAGCG GGAGAATGAT TCACTTAAGG ATAAAGTTGA GAACCTTGAA 
AGGGAATTGC AGATGTCAGA AGAAAACCAG GAGCTAGTGA TTCTTGATGC CGAGAATTCC 
AAAGCAGAAG TAGAGACTCT AAAAACACAA ATAGAAGAGA TGGCCAGAAG CCTGAAAGTT 
TTTGAATTAG ACCTTGTCAC GTTAAGGTCT GAAAAAGAAA ATCTGACAAA ACAAATACAA 
GAAAAACAAG GTCAGTTGTC AGAACTAGAC AAGTTACTCT CTTCATTTAA AAGTCTGTTA 
GAAGAAAAGG AGCAAGCAGA GATACAGATC AAAGAAGAAT CTAAAACTGC AGTGGAGATG 
CTTCAGAATC AGTTAAAGGA GCTAAATGAG GCAGTAGCAG CCTTGTGTGG TGACCAAGAA 
ATTATGAAGG CCACAGAACA GAGTCTAGAC CCACCAATAG AGGAAGAGCA TCAGCTGAGA 
AATAGCATTG AAAAGCTGAG AGCCCGCCTA GAAGCTGATG AAAAGAAGCA GCTCTGTGTC 
TTACAACAAC TGAAGGAAAG TGAGCATCAT GCAGATTTAC TTAAGGGTAG AGTGGAGAAC 
CTTGAAAGAG AGCTAGAGAT AGCCAGGACA AACCAAGAGC ATGCAGCTCT TGAGGCAGAG 
AATTCCAAAG GAGAGGTAGA GACCCTAAAA GCAAAAATAG AAGGGATGAC CCAAAGTCTG 
AGAGGTCTGG AATTAGATGT TGTTACTATA AGGTCAGAAA AAGAAAATCT GACAAATGAA 
TTACAAAAAG AGCAAGAGCG AATATCTGAA TTAGAAATAA TAAATTCATC ATTTGAAAAT 
ATTTTGCAAG AAAAAGAGCA AGAGAAAGTA CAGATGAAAG AAAAATCAAG CACTGCCATG 
GAGATGCTTC AAACACAATT AAAAGAGCTC AATGAGAGAG TGGCAGCCCT GCATAATGAC 
CAAGAAGCCT GTAAGGCCAA AGAGCAGAAT CTTAGTAGTC AAGTAGAGTG TCTTGAACTT 
GAGAAGGCTC AGTTGCTACA AGGCCTTGAT GAGGCCAAAA ATAATTATAT TGTTTTGCAA 
TCTTCAGTGA ATGGCCTCAT TCAAGAAGTA GAAGATGGCA AGCAGAAACT GGAGAAGAAG 
GATGAAGAAA TCAGTAGACT GAAAAATCAA ATTCAAGACC AAGAGCAGCT TGTCTCTAAA 
CTGTCCCAGG TGGAAGGAGA GCACCAACTT TGGAAGGAGC AAAACTTAGA ACTGAGAAAT 
CTGACAGTGG AATTGGAGCA GAAGATCCAA GTGCTACAAT CCAAAAATGC CTCTTTGCAG 
GACACATTAG AAGTGCTGCA GAGTTCTTAC AAGAATCTAG AGAATGAGCT TGAATTGACA 
AAAATGGACA AAATGTCCTT TGTTGAAAAA GTAAACAAAA TGACTGCAAA GGAAACTGAG 
CTGCAGAGGG AAATGCATGA GATGGCACAG AAAACAGCAG AGCTGCAAGA AGAACTCAGT 
GGAGAGAAAA ATAGGCTAGC TGGAGAGTTG CAGTTACTGT TGGAAGAAAT AAAGAGCAGC 
AAATATGAAG TAGAAATCCA GACATACCGA GAGAAATTGA CTTCTAAAGA AGAATGTCTC 
AGTTCACAGA AGCTGGAGAT AGACCTTTTA AAGTCTAGTA AAGAAGAGCT CAATAATTCA 
TTGAAAGCTA CTACTCAGAT TTTGGAAGAA TTGAAGAAAA CCAAGATGGA CAATCTAAAA 
TATGTAAATC AGTTGAAGAA GGAAAATGAA CGTGCCCAGG GGAAAATGAA GTTGTTGATC 
AAATCCTGTA AACAGCTGGA AGAGGAAAAG GAGATACTGC AGAAAGAACT CTCTCAACTT 
CAAGCTGCAC AGGAGAAGCA GAAAACAGGT ACTGTTATGG ATACCAAGGT CGATGAATTA 
ACAACTGAGA TCAAAGAACT GAAAGAAACT CTTGAAGAAA AAACCAAGGA GGCAGATGAA 
TACTTGGATA AGTACTGTTC CTTGCTTATA AGCCATGAAA AGTTAGAGAA AGCTAAAGAG 
ATGTTAGAGA CACAAGTGGC CCATCTGTGT TCACAGCAAT CTAAACAAGA TTCCCGAGGG 
TCTCCTTTGC TAGGTCCAGT TGTTCCAGGA CCATCTCCAA TCCCTTCTGT TACTGAAAAG 
AGGTTATCAT CTGGCCAAAA TAAAGCTTCA GGCAAGAGGC AAAGATCCAG TGGAATATGG 
GAGAATGGTA GAGGACCAAC ACCTGCTACC CCAGAGAGCT TTTCTAAAAA AAGCAAGAAA 
GCAGTCATGA GTGGTATTCA CCCTGCAGAA GACACGGAAG GTACTGAGTT TGAGCCAGAG 
GGACTTCCAG AAGTTGTAAA GAAAGGGTTT GCTGACATCC CGACAGGAAA GACTAGCCCA 
TATATCCTGC GAAGAACAAC CATGGCAACT CGGACCAGCC CCCGCCTGGC TGCACAGAAG 
TTAGCGCTAT CCCCACTGAG TCTCGGCAAA GAAAATCTTG CAGAGTCCTC CAAACCAACA 
GCTGGTGGCA GCAGATCACA AAAGGTCAAA GTTGCTCAGC GGAGCCCAGT AGATTCAGGC 
ACCATCCTCC GAGAACCCAC CACGAAATCC GTCCCAGTCA ATAATCTTCC TGAGAGAAGT 
CCGACTGACA GCCCCAGAGA GGGCCTGAGG GTCAAGCGAG GCCGACTTGT CCCCAGCCCC 
AAAGCTGGAC TGGAGTCCAA CGGCAGTGAG AACTGTAAGG TCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011507384.1
CDS	169..9336
Translation	MSWALEEWKEGLPTRALQKIQELEGQLDKLKKEKQQRQFQLDSLEAALQKQKQKVENEKTEGTNLKRENQRLMEICESLEKTKQKISHELQVKESQVNFQEGQLNSGKKQIEKLEQELKRCKSELERSQQAAQSADVSLNPCNTPQKIFTTPLTPSQYYSGSKYEDLKEKYNKEVEERKRLEAEVKALQAKKASQTLPQATMNHRDIARHQASSSVFSWQQEKTPSHLSSNSQRTPIRRDFSASYFSGEQEVTPSRSTLQIGKRDANSSFFDNSSSPHLLDQLKAQNQELRNKINELELRLQGHEKEMKGQVNKFQELQLQLEKAKVELIEKEKVLNKCRDELVRTTAQYDQASTKYTALEQKLKKLTEDLSCQRQNAESARCSLEQKIKEKEKEFQEELSRQQRSFQTLDQECIQMKARLTQELQQAKNMHNVLQAELDKLTSVKQQLENNLEEFKQKLCRAEQAFQASQIKENELRRSMEEMKKENNLLKSHSEQKAREVCHLEAELKNIKQCLNQSQNFAEEMKAKNTSQETMLRDLQEKINQQENSLTLEKLKLAVADLEKQRDCSQDLLKKREHHIEQLNDKLSKTEKESKALLSALELKKKEYEELKEEKTLFSCWKSENEKLLTQMESEKENLQSKINHLETCLKTQQIKSHEYNERVRTLEMDRENLSVEIRNLHNVLDSKSVEVETQKLAYMELQQKAEFSDQKHQKEIENMCLKTSQLTGQVEDLEHKLQLLSNEIMDKDRCYQDLHAEYESLRDLLKSKDASLVTNEDHQRSLLAFDQQPAMHHSFANIIGEQGSMPSERSECRLEADQSPKNSAILQNRVDSLEFSLESQKQMNSDLQKQCEELVQIKGEIEENLMKAEQMHQSFVAETSQRISKLQEDTSAHQNVVAETLSALENKEKELQLLNDKVETEQAEIQELKKSNHLLEDSLKELQLLSETLSLEKKEMSSIISLNKREIEELTQENGTLKEINASLNQEKMNLIQKSESFANYIDEREKSISELSDQYKQEKLILLQRCEETGNAYEDLSQKYKAAQEKNSKLECLLNECTSLCENRKNELEQLKEAFAKEHQEFLTKLAFAEERNQNLMLELETVQQALRSEMTDNQNNSKSEAGGLKQEIMTLKEEQNKMQKEVNDLLQENEQLMKVMKTKHECQNLESEPIRNSVKERESERNQCNFKPQMDLEVKEISLDSYNAQLVQLEAMLRNKELKLQESEKEKECLQHELQTIRGDLETSNLQDMQSQEISGLKDCEIDAEEKYISGPHELSTSQNDNAHLQCSLQTTMNKLNELEKICEILQAEKYELVTELNDSRSECITATRKMAEEVGKLLNEVKILNDDSGLLHGELVEDIPGGEFGEQPNEQHPVSLAPLDESNSYEHLTLSDKEVQMHFAELQEKFLSLQSEHKILHDQHCQMSSKMSELQTYVDSLKAENLVLSTNLRNFQGDLVKEMQLGLEEGLVPSLSSSCVPDSSSLSSLGDSSFYRALLEQTGDMSLLSNLEGAVSANQCSVDEVFCSSLQEENLTRKETPSAPAKGVEELESLCEVYRQSLEKLEEKMESQGIMKNKEIQELEQLLSSERQELDCLRKQYLSENEQWQQKLTSVTLEMESKLAAEKKQTEQLSLELEVARLQLQGLDLSSRSLLGIDTEDAIQGRNESCDISKEHTSETTERTPKHDVHQICDKDAQQDLNLDIEKITETGAVKPTGECSGEQSPDTNYEPPGEDKTQGSSECISELSFSGPNALVPMDFLGNQEDIHNLQLRVKETSNENLRLLHVIEDRDRKVESLLNEMKELDSKLHLQEVQLMTKIEACIELEKIVGELKKENSDLSEKLEYFSCDHQELLQRVETSEGLNSDLEMHADKSSREDIGDNVAKVNDSWKERFLDVENELSRIRSEKASIEHEALYLEADLEVVQTEKLCLEKDNENKQKVIVCLEEELSVVTSERNQLRGELDTMSKKTTALDQLSEKMKEKTQELESHQSECLHCIQVAEAEVKEKTELLQTLSSDVSELLKDKTHLQEKLQSLEKDSQALSLTKCELENQIAQLNKEKELLVKESESLQARLSESDYEKLNVSKALEAALVEKGEFALRLSSTQEEVHQLRRGIEKLRVRIEADEKKQLHIAEKLKERERENDSLKDKVENLERELQMSEENQELVILDAENSKAEVETLKTQIEEMARSLKVFELDLVTLRSEKENLTKQIQEKQGQLSELDKLLSSFKSLLEEKEQAEIQIKEESKTAVEMLQNQLKELNEAVAALCGDQEIMKATEQSLDPPIEEEHQLRNSIEKLRARLEADEKKQLCVLQQLKESEHHADLLKGRVENLERELEIARTNQEHAALEAENSKGEVETLKAKIEGMTQSLRGLELDVVTIRSEKENLTNELQKEQERISELEIINSSFENILQEKEQEKVQMKEKSSTAMEMLQTQLKELNERVAALHNDQEACKAKEQNLSSQVECLELEKAQLLQGLDEAKNNYIVLQSSVNGLIQEVEDGKQKLEKKDEEISRLKNQIQDQEQLVSKLSQVEGEHQLWKEQNLELRNLTVELEQKIQVLQSKNASLQDTLEVLQSSYKNLENELELTKMDKMSFVEKVNKMTAKETELQREMHEMAQKTAELQEELSGEKNRLAGELQLLLEEIKSSKYEVEIQTYREKLTSKEECLSSQKLEIDLLKSSKEELNNSLKATTQILEELKKTKMDNLKYVNQLKKENERAQGKMKLLIKSCKQLEEEKEILQKELSQLQAAQEKQKTGTVMDTKVDELTTEIKELKETLEEKTKEADEYLDKYCSLLISHEKLEKAKEMLETQVAHLCSQQSKQDSRGSPLLGPVVPGPSPIPSVTEKRLSSGQNKASGKRQRSSGIWENGRGPTPATPESFSKKSKKAVMSGIHPAEDTEGTEFEPEGLPEVVKKGFADIPTGKTSPYILRRTTMATRTSPRLAAQKLALSPLSLGKENLAESSKPTAGGSRSQKVKVAQRSPVDSGTILREPTTKSVPVNNLPERSPTDSPREGLRVKRGRLVPSPKAGLESNGSENCKVQ

Target ORF information:

RefSeq Version	XM_011509082.3
Organism	Homo sapiens(human)
Definition	Homo sapiens centromere protein F (CENPF), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011509082.3

ORF Insert Sequence:

ATGAGCTGGG CTTTGGAAGA ATGGAAAGAA GGGCTGCCTA CAAGAGCTCT TCAGAAAATT 
CAAGAGCTTG AAGGACAGCT TGACAAACTG AAGAAGGAAA AGCAGCAAAG GCAGTTTCAG 
CTTGACAGTC TCGAGGCTGC GCTGCAGAAG CAAAAACAGA AGGTTGAAAA TGAAAAAACC 
GAGGGTACAA ACCTGAAAAG GGAGAATCAA AGATTGATGG AAATATGTGA AAGTCTGGAG 
AAAACTAAGC AGAAGATTTC TCATGAACTT CAAGTCAAGG AGTCACAAGT GAATTTCCAG 
GAAGGACAAC TGAATTCAGG CAAAAAACAA ATAGAAAAAC TGGAACAGGA ACTTAAAAGG 
TGTAAATCTG AGCTTGAAAG AAGCCAACAA GCTGCGCAGT CTGCAGATGT CTCTCTGAAT 
CCATGCAATA CACCACAAAA AATTTTTACA ACTCCACTAA CACCAAGTCA ATATTATAGT 
GGTTCCAAGT ATGAAGATCT AAAAGAAAAA TATAATAAAG AGGTTGAAGA ACGAAAAAGA 
TTAGAGGCAG AGGTTAAAGC CTTGCAGGCT AAAAAAGCAA GCCAGACTCT TCCACAAGCC 
ACCATGAATC ACCGCGACAT TGCCCGGCAT CAGGCTTCAT CATCTGTGTT CTCATGGCAG 
CAAGAGAAGA CCCCAAGTCA TCTTTCATCT AATTCTCAAA GAACTCCAAT TAGGAGAGAT 
TTCTCTGCAT CTTACTTTTC TGGGGAACAA GAGGTGACTC CAAGTCGATC AACTTTGCAA 
ATAGGGAAAA GAGATGCTAA TAGCAGTTTC TTTGACAATT CTAGCAGTCC TCATCTTTTG 
GATCAATTAA AAGCGCAGAA TCAAGAGCTA AGAAACAAGA TTAATGAGTT GGAACTACGC 
CTGCAAGGAC ATGAAAAAGA AATGAAAGGC CAAGTGAATA AGTTTCAAGA ACTCCAACTC 
CAACTGGAGA AAGCAAAAGT GGAATTAATT GAAAAAGAGA AAGTTTTGAA CAAATGTAGG 
GATGAACTAG TGAGAACAAC AGCACAATAC GACCAGGCGT CAACCAAGTA TACTGCATTG 
GAACAAAAAC TGAAAAAATT GACGGAAGAT TTGAGTTGTC AGCGACAAAA TGCAGAAAGT 
GCCAGATGTT CTCTGGAACA GAAAATTAAG GAAAAAGAAA AGGAGTTTCA AGAGGAGCTC 
TCCCGTCAAC AGCGTTCTTT CCAAACACTG GACCAGGAGT GCATCCAGAT GAAGGCCAGA 
CTCACCCAGG AGTTACAGCA AGCCAAGAAT ATGCACAACG TCCTGCAGGC TGAACTGGAT 
AAACTCACAT CAGTAAAGCA ACAGCTAGAA AACAATTTGG AAGAGTTTAA GCAAAAGTTG 
TGCAGAGCTG AACAGGCGTT CCAGGCGAGT CAGATCAAGG AGAATGAGCT GAGGAGAAGC 
ATGGAGGAAA TGAAGAAGGA AAACAACCTC CTTAAGAGTC ACTCTGAGCA AAAGGCCAGA 
GAAGTCTGCC ACCTGGAGGC AGAACTCAAG AACATCAAAC AGTGTTTAAA TCAGAGCCAG 
AATTTTGCAG AAGAAATGAA AGCGAAGAAT ACCTCTCAGG AAACCATGTT AAGAGATCTT 
CAAGAAAAAA TAAATCAGCA AGAAAACTCC TTGACTTTAG AAAAACTGAA GCTTGCTGTG 
GCTGATCTGG AAAAGCAGCG AGATTGTTCT CAAGACCTTT TGAAGAAAAG AGAACATCAC 
ATTGAACAAC TTAATGATAA GTTAAGCAAG ACAGAGAAAG AGTCCAAAGC CTTGCTGAGT 
GCTTTAGAGT TAAAAAAGAA AGAATATGAA GAATTGAAAG AAGAGAAAAC TCTGTTTTCT 
TGTTGGAAAA GTGAAAACGA AAAACTTTTA ACTCAGATGG AATCAGAAAA GGAAAACTTG 
CAGAGTAAAA TTAATCACTT GGAAACTTGT CTGAAGACAC AGCAAATAAA AAGTCATGAA 
TACAACGAGA GAGTAAGAAC GCTGGAGATG GACAGAGAAA ACCTAAGTGT CGAGATCAGA 
AACCTTCACA ACGTGTTAGA CAGTAAGTCA GTGGAGGTAG AGACCCAGAA ACTAGCTTAT 
ATGGAGCTAC AGCAGAAAGC TGAGTTCTCA GATCAGAAAC ATCAGAAGGA AATAGAAAAT 
ATGTGTTTGA AGACTTCTCA GCTTACTGGG CAAGTTGAAG ATCTAGAACA CAAGCTTCAG 
TTACTGTCAA ATGAAATAAT GGACAAAGAC CGGTGTTACC AAGACTTGCA TGCCGAATAT 
GAGAGCCTCA GGGATCTGCT AAAATCCAAA GATGCTTCTC TGGTGACAAA TGAAGATCAT 
CAGAGAAGTC TTTTGGCTTT TGATCAGCAG CCTGCCATGC ATCATTCCTT TGCAAATATA 
ATTGGAGAAC AAGGAAGCAT GCCTTCAGAG AGGAGTGAAT GTCGTTTAGA AGCAGACCAA 
AGTCCGAAAA ATTCTGCCAT CCTACAAAAT AGAGTTGATT CACTTGAATT TTCATTAGAG 
TCTCAAAAAC AGATGAACTC AGACCTGCAA AAGCAGTGTG AAGAGTTGGT GCAAATCAAA 
GGAGAAATAG AAGAAAATCT CATGAAAGCA GAACAGATGC ATCAAAGTTT TGTGGCTGAA 
ACAAGTCAGC GCATTAGTAA GTTACAGGAA GACACTTCTG CTCACCAGAA TGTTGTTGCT 
GAAACCTTAA GTGCCCTTGA GAACAAGGAA AAAGAGCTGC AACTTTTAAA TGATAAGGTA 
GAAACTGAGC AGGCAGAGAT TCAAGAATTA AAAAAGAGCA ACCATCTACT TGAAGACTCT 
CTAAAGGAGC TACAACTTTT ATCCGAAACC CTAAGCTTGG AGAAGAAAGA AATGAGTTCC 
ATCATTTCTC TAAATAAAAG GGAAATTGAA GAGCTGACCC AAGAGAATGG GACTCTTAAG 
GAAATTAATG CATCCTTAAA TCAAGAGAAG ATGAACTTAA TCCAGAAAAG TGAGAGTTTT 
GCAAACTATA TAGATGAAAG GGAGAAAAGC ATTTCAGAGT TATCTGATCA GTACAAGCAA 
GAAAAACTTA TTTTACTACA AAGATGTGAA GAAACCGGAA ATGCATATGA GGATCTTAGT 
CAAAAATACA AAGCAGCACA GGAAAAGAAT TCTAAATTAG AATGCTTGCT AAATGAATGC 
ACTAGTCTTT GTGAAAATAG GAAAAATGAG TTGGAACAGC TAAAGGAAGC ATTTGCAAAG 
GAACACCAAG AATTCTTAAC AAAATTAGCA TTTGCTGAAG AAAGAAATCA GAATCTGATG 
CTAGAGTTGG AGACAGTGCA GCAAGCTCTG AGATCTGAGA TGACAGATAA CCAAAACAAT 
TCTAAGAGCG AGGCTGGTGG TTTAAAGCAA GAAATCATGA CTTTAAAGGA AGAACAAAAC 
AAAATGCAAA AGGAAGTTAA TGACTTATTA CAAGAGAATG AACAGCTGAT GAAGGTAATG 
AAGACTAAAC ATGAATGTCA AAATCTAGAA TCAGAACCAA TTAGGAACTC TGTGAAAGAA 
AGAGAGAGTG AGAGAAATCA ATGTAATTTT AAACCTCAGA TGGATCTTGA AGTTAAAGAA 
ATTTCTCTAG ATAGTTATAA TGCGCAGTTG GTGCAATTAG AAGCTATGCT AAGAAATAAG 
GAATTAAAAC TTCAGGAAAG TGAGAAGGAG AAGGAGTGCC TGCAGCATGA ATTACAGACA 
ATTAGAGGAG ATCTTGAAAC CAGCAATTTG CAAGACATGC AGTCACAAGA AATTAGTGGC 
CTTAAAGACT GTGAAATAGA TGCGGAAGAA AAGTATATTT CAGGGCCTCA TGAGTTGTCA 
ACAAGTCAAA ACGACAATGC ACACCTTCAG TGCTCTCTGC AAACAACAAT GAACAAGCTG 
AATGAGCTAG AGAAAATATG TGAAATACTG CAGGCTGAAA AGTATGAACT CGTAACTGAG 
CTGAATGATT CAAGGTCAGA ATGTATCACA GCAACTAGGA AAATGGCAGA AGAGGTAGGG 
AAACTACTAA ATGAAGTTAA AATATTAAAT GATGACAGTG GTCTTCTCCA TGGTGAGTTA 
GTGGAAGACA TACCAGGAGG TGAATTTGGT GAACAACCAA ATGAACAGCA CCCTGTGTCT 
TTGGCTCCAT TGGACGAGAG TAATTCCTAC GAGCACTTGA CATTGTCAGA CAAAGAAGTT 
CAAATGCACT TTGCCGAATT GCAAGAGAAA TTCTTATCTT TACAAAGTGA ACACAAAATT 
TTACATGATC AGCACTGTCA GATGAGCTCT AAAATGTCAG AGCTGCAGAC CTATGTTGAC 
TCATTAAAGG CCGAAAATTT GGTCTTGTCA ACGAATCTGA GAAACTTTCA AGGTGACTTG 
GTGAAGGAGA TGCAGCTGGG CTTGGAGGAG GGGCTCGTTC CATCCCTGTC ATCCTCTTGT 
GTGCCTGACA GCTCTAGTCT TAGCAGTTTG GGAGACTCCT CCTTTTACAG AGCTCTTTTA 
GAACAGACAG GAGATATGTC TCTTTTGAGT AATTTAGAAG GGGCTGTTTC AGCAAACCAG 
TGCAGTGTAG ATGAAGTATT TTGCAGCAGT CTGCAGGAGG AGAATCTGAC CAGGAAAGAA 
ACCCCTTCGG CCCCAGCGAA GGGTGTTGAA GAGCTTGAGT CCCTCTGTGA GGTGTACCGG 
CAGTCCCTCG AGAAGCTAGA AGAGAAAATG GAAAGTCAAG GGATTATGAA AAATAAGGAA 
ATTCAAGAGC TCGAGCAGTT ATTAAGTTCT GAAAGGCAAG AGCTTGACTG CCTTAGGAAG 
CAGTATTTGT CAGAAAATGA ACAGTGGCAA CAGAAGCTGA CAAGCGTGAC TCTGGAGATG 
GAGTCCAAGT TGGCGGCAGA AAAGAAACAG ACGGAACAAC TGTCACTTGA GCTGGAAGTA 
GCACGACTCC AGCTACAAGG TCTGGACTTA AGTTCTCGGT CTTTGCTTGG CATCGACACA 
GAAGATGCTA TTCAAGGCCG AAATGAGAGC TGTGACATAT CAAAAGAACA TACTTCAGAA 
ACTACAGAAA GAACACCAAA GCATGATGTT CATCAGATTT GTGATAAAGA TGCTCAGCAG 
GACCTCAATC TAGACATTGA GAAAATAACT GAGACTGGTG CAGTGAAACC CACAGGAGAG 
TGCTCTGGGG AACAGTCCCC AGATACCAAT TATGAGCCTC CAGGGGAAGA TAAAACCCAG 
GGCTCTTCAG AATGCATTTC TGAATTGTCA TTTTCTGGTC CTAATGCTTT GGTACCTATG 
GATTTCCTGG GGAATCAGGA AGATATCCAT AATCTTCAAC TGCGGGTAAA AGAGACATCA 
AATGAGAATT TGAGATTACT TCATGTGATA GAGGACCGTG ACAGAAAAGT TGAAAGTTTG 
CTAAATGAAA TGAAAGAATT AGACTCAAAA CTCCATTTAC AGGAGGTACA ACTAATGACC 
AAAATTGAAG CATGCATAGA ATTGGAAAAA ATAGTTGGGG AACTTAAGAA AGAAAACTCA 
GATTTAAGTG AAAAATTGGA ATATTTTTCT TGTGATCACC AGGAGTTACT CCAGAGAGTA 
GAAACTTCTG AAGGCCTCAA TTCTGATTTA GAAATGCATG CAGATAAATC ATCACGTGAA 
GATATTGGAG ATAATGTGGC CAAGGTGAAT GACAGCTGGA AGGAGAGATT TCTTGATGTG 
GAAAATGAGC TGAGTAGGAT CAGATCGGAG AAAGCTAGCA TTGAGCATGA AGCCCTCTAC 
CTGGAGGCTG ACTTAGAGGT AGTTCAAACA GAGAAGCTAT GTTTAGAAAA AGACAATGAA 
AATAAGCAGA AGGTTATTGT CTGCCTTGAA GAAGAACTCT CAGTGGTCAC AAGTGAGAGA 
AACCAGCTTC GTGGAGAATT AGATACTATG TCAAAAAAAA CCACGGCACT GGATCAGTTG 
TCTGAAAAAA TGAAGGAGAA AACACAAGAG CTTGAGTCTC ATCAAAGTGA GTGTCTCCAT 
TGCATTCAGG TGGCAGAGGC AGAGGTGAAG GAAAAGACGG AACTCCTTCA GACTTTGTCC 
TCTGATGTGA GTGAGCTGTT AAAAGACAAA ACTCATCTCC AGGAAAAGCT GCAGAGTTTG 
GAAAAGGACT CACAGGCACT GTCTTTGACA AAATGTGAGC TGGAAAACCA AATTGCACAA 
CTGAATAAAG AGAAAGAATT GCTTGTCAAG GAATCTGAAA GCCTGCAGGC CAGACTGAGT 
GAATCAGATT ATGAAAAGCT GAATGTCTCC AAGGCCTTGG AGGCCGCACT GGTGGAGAAA 
GGTGAGTTCG CATTGAGGCT GAGCTCAACA CAGGAGGAAG TGCATCAGCT GAGAAGAGGC 
ATCGAGAAAC TGAGAGTTCG CATTGAGGCC GATGAAAAGA AGCAGCTGCA CATCGCAGAG 
AAACTGAAAG AACGCGAGCG GGAGAATGAT TCACTTAAGG ATAAAGTTGA GAACCTTGAA 
AGGGAATTGC AGATGTCAGA AGAAAACCAG GAGCTAGTGA TTCTTGATGC CGAGAATTCC 
AAAGCAGAAG TAGAGACTCT AAAAACACAA ATAGAAGAGA TGGCCAGAAG CCTGAAAGTT 
TTTGAATTAG ACCTTGTCAC GTTAAGGTCT GAAAAAGAAA ATCTGACAAA ACAAATACAA 
GAAAAACAAG GTCAGTTGTC AGAACTAGAC AAGTTACTCT CTTCATTTAA AAGTCTGTTA 
GAAGAAAAGG AGCAAGCAGA GATACAGATC AAAGAAGAAT CTAAAACTGC AGTGGAGATG 
CTTCAGAATC AGTTAAAGGA GCTAAATGAG GCAGTAGCAG CCTTGTGTGG TGACCAAGAA 
ATTATGAAGG CCACAGAACA GAGTCTAGAC CCACCAATAG AGGAAGAGCA TCAGCTGAGA 
AATAGCATTG AAAAGCTGAG AGCCCGCCTA GAAGCTGATG AAAAGAAGCA GCTCTGTGTC 
TTACAACAAC TGAAGGAAAG TGAGCATCAT GCAGATTTAC TTAAGGGTAG AGTGGAGAAC 
CTTGAAAGAG AGCTAGAGAT AGCCAGGACA AACCAAGAGC ATGCAGCTCT TGAGGCAGAG 
AATTCCAAAG GAGAGGTAGA GACCCTAAAA GCAAAAATAG AAGGGATGAC CCAAAGTCTG 
AGAGGTCTGG AATTAGATGT TGTTACTATA AGGTCAGAAA AAGAAAATCT GACAAATGAA 
TTACAAAAAG AGCAAGAGCG AATATCTGAA TTAGAAATAA TAAATTCATC ATTTGAAAAT 
ATTTTGCAAG AAAAAGAGCA AGAGAAAGTA CAGATGAAAG AAAAATCAAG CACTGCCATG 
GAGATGCTTC AAACACAATT AAAAGAGCTC AATGAGAGAG TGGCAGCCCT GCATAATGAC 
CAAGAAGCCT GTAAGGCCAA AGAGCAGAAT CTTAGTAGTC AAGTAGAGTG TCTTGAACTT 
GAGAAGGCTC AGTTGCTACA AGGCCTTGAT GAGGCCAAAA ATAATTATAT TGTTTTGCAA 
TCTTCAGTGA ATGGCCTCAT TCAAGAAGTA GAAGATGGCA AGCAGAAACT GGAGAAGAAG 
GATGAAGAAA TCAGTAGACT GAAAAATCAA ATTCAAGACC AAGAGCAGCT TGTCTCTAAA 
CTGTCCCAGG TGGAAGGAGA GCACCAACTT TGGAAGGAGC AAAACTTAGA ACTGAGAAAT 
CTGACAGTGG AATTGGAGCA GAAGATCCAA GTGCTACAAT CCAAAAATGC CTCTTTGCAG 
GACACATTAG AAGTGCTGCA GAGTTCTTAC AAGAATCTAG AGAATGAGCT TGAATTGACA 
AAAATGGACA AAATGTCCTT TGTTGAAAAA GTAAACAAAA TGACTGCAAA GGAAACTGAG 
CTGCAGAGGG AAATGCATGA GATGGCACAG AAAACAGCAG AGCTGCAAGA AGAACTCAGT 
GGAGAGAAAA ATAGGCTAGC TGGAGAGTTG CAGTTACTGT TGGAAGAAAT AAAGAGCAGC 
AAATATGAAG TAGAAATCCA GACATACCGA GAGAAATTGA CTTCTAAAGA AGAATGTCTC 
AGTTCACAGA AGCTGGAGAT AGACCTTTTA AAGTCTAGTA AAGAAGAGCT CAATAATTCA 
TTGAAAGCTA CTACTCAGAT TTTGGAAGAA TTGAAGAAAA CCAAGATGGA CAATCTAAAA 
TATGTAAATC AGTTGAAGAA GGAAAATGAA CGTGCCCAGG GGAAAATGAA GTTGTTGATC 
AAATCCTGTA AACAGCTGGA AGAGGAAAAG GAGATACTGC AGAAAGAACT CTCTCAACTT 
CAAGCTGCAC AGGAGAAGCA GAAAACAGGT ACTGTTATGG ATACCAAGGT CGATGAATTA 
ACAACTGAGA TCAAAGAACT GAAAGAAACT CTTGAAGAAA AAACCAAGGA GGCAGATGAA 
TACTTGGATA AGTACTGTTC CTTGCTTATA AGCCATGAAA AGTTAGAGAA AGCTAAAGAG 
ATGTTAGAGA CACAAGTGGC CCATCTGTGT TCACAGCAAT CTAAACAAGA TTCCCGAGGG 
TCTCCTTTGC TAGGTCCAGT TGTTCCAGGA CCATCTCCAA TCCCTTCTGT TACTGAAAAG 
AGGTTATCAT CTGGCCAAAA TAAAGCTTCA GGCAAGAGGC AAAGATCCAG TGGAATATGG 
GAGAATGGTA GAGGACCAAC ACCTGCTACC CCAGAGAGCT TTTCTAAAAA AAGCAAGAAA 
GCAGTCATGA GTGGTATTCA CCCTGCAGAA GACACGGAAG GTACTGAGTT TGAGCCAGAG 
GGACTTCCAG AAGTTGTAAA GAAAGGGTTT GCTGACATCC CGACAGGAAA GACTAGCCCA 
TATATCCTGC GAAGAACAAC CATGGCAACT CGGACCAGCC CCCGCCTGGC TGCACAGAAG 
TTAGCGCTAT CCCCACTGAG TCTCGGCAAA GAAAATCTTG CAGAGTCCTC CAAACCAACA 
GCTGGTGGCA GCAGATCACA AAAGGTCAAA GTTGCTCAGC GGAGCCCAGT AGATTCAGGC 
ACCATCCTCC GAGAACCCAC CACGAAATCC GTCCCAGTCA ATAATCTTCC TGAGAGAAGT 
CCGACTGACA GCCCCAGAGA GGGCCTGAGG GTCAAGCGAG GCCGACTTGT CCCCAGCCCC 
AAAGCTGGAC TGGAGTCCAA CGGCAGTGAG AACTGTAAGG TCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CENPF ( XM_011509082.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011507384.1 Homo sapiens centromere protein F (CENPF), transcript variant X2, mRNA.

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CloneID	OHu56821
Clone ID Related Accession (Same CDS sequence)	XM_011509082.3 , XM_011509082.2
Accession Version	XM_011509082.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9168bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	centromere protein F isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011509082.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTGGG CTTTGGAAGA ATGGAAAGAA GGGCTGCCTA CAAGAGCTCT TCAGAAAATT 
CAAGAGCTTG AAGGACAGCT TGACAAACTG AAGAAGGAAA AGCAGCAAAG GCAGTTTCAG 
CTTGACAGTC TCGAGGCTGC GCTGCAGAAG CAAAAACAGA AGGTTGAAAA TGAAAAAACC 
GAGGGTACAA ACCTGAAAAG GGAGAATCAA AGATTGATGG AAATATGTGA AAGTCTGGAG 
AAAACTAAGC AGAAGATTTC TCATGAACTT CAAGTCAAGG AGTCACAAGT GAATTTCCAG 
GAAGGACAAC TGAATTCAGG CAAAAAACAA ATAGAAAAAC TGGAACAGGA ACTTAAAAGG 
TGTAAATCTG AGCTTGAAAG AAGCCAACAA GCTGCGCAGT CTGCAGATGT CTCTCTGAAT 
CCATGCAATA CACCACAAAA AATTTTTACA ACTCCACTAA CACCAAGTCA ATATTATAGT 
GGTTCCAAGT ATGAAGATCT AAAAGAAAAA TATAATAAAG AGGTTGAAGA ACGAAAAAGA 
TTAGAGGCAG AGGTTAAAGC CTTGCAGGCT AAAAAAGCAA GCCAGACTCT TCCACAAGCC 
ACCATGAATC ACCGCGACAT TGCCCGGCAT CAGGCTTCAT CATCTGTGTT CTCATGGCAG 
CAAGAGAAGA CCCCAAGTCA TCTTTCATCT AATTCTCAAA GAACTCCAAT TAGGAGAGAT 
TTCTCTGCAT CTTACTTTTC TGGGGAACAA GAGGTGACTC CAAGTCGATC AACTTTGCAA 
ATAGGGAAAA GAGATGCTAA TAGCAGTTTC TTTGACAATT CTAGCAGTCC TCATCTTTTG 
GATCAATTAA AAGCGCAGAA TCAAGAGCTA AGAAACAAGA TTAATGAGTT GGAACTACGC 
CTGCAAGGAC ATGAAAAAGA AATGAAAGGC CAAGTGAATA AGTTTCAAGA ACTCCAACTC 
CAACTGGAGA AAGCAAAAGT GGAATTAATT GAAAAAGAGA AAGTTTTGAA CAAATGTAGG 
GATGAACTAG TGAGAACAAC AGCACAATAC GACCAGGCGT CAACCAAGTA TACTGCATTG 
GAACAAAAAC TGAAAAAATT GACGGAAGAT TTGAGTTGTC AGCGACAAAA TGCAGAAAGT 
GCCAGATGTT CTCTGGAACA GAAAATTAAG GAAAAAGAAA AGGAGTTTCA AGAGGAGCTC 
TCCCGTCAAC AGCGTTCTTT CCAAACACTG GACCAGGAGT GCATCCAGAT GAAGGCCAGA 
CTCACCCAGG AGTTACAGCA AGCCAAGAAT ATGCACAACG TCCTGCAGGC TGAACTGGAT 
AAACTCACAT CAGTAAAGCA ACAGCTAGAA AACAATTTGG AAGAGTTTAA GCAAAAGTTG 
TGCAGAGCTG AACAGGCGTT CCAGGCGAGT CAGATCAAGG AGAATGAGCT GAGGAGAAGC 
ATGGAGGAAA TGAAGAAGGA AAACAACCTC CTTAAGAGTC ACTCTGAGCA AAAGGCCAGA 
GAAGTCTGCC ACCTGGAGGC AGAACTCAAG AACATCAAAC AGTGTTTAAA TCAGAGCCAG 
AATTTTGCAG AAGAAATGAA AGCGAAGAAT ACCTCTCAGG AAACCATGTT AAGAGATCTT 
CAAGAAAAAA TAAATCAGCA AGAAAACTCC TTGACTTTAG AAAAACTGAA GCTTGCTGTG 
GCTGATCTGG AAAAGCAGCG AGATTGTTCT CAAGACCTTT TGAAGAAAAG AGAACATCAC 
ATTGAACAAC TTAATGATAA GTTAAGCAAG ACAGAGAAAG AGTCCAAAGC CTTGCTGAGT 
GCTTTAGAGT TAAAAAAGAA AGAATATGAA GAATTGAAAG AAGAGAAAAC TCTGTTTTCT 
TGTTGGAAAA GTGAAAACGA AAAACTTTTA ACTCAGATGG AATCAGAAAA GGAAAACTTG 
CAGAGTAAAA TTAATCACTT GGAAACTTGT CTGAAGACAC AGCAAATAAA AAGTCATGAA 
TACAACGAGA GAGTAAGAAC GCTGGAGATG GACAGAGAAA ACCTAAGTGT CGAGATCAGA 
AACCTTCACA ACGTGTTAGA CAGTAAGTCA GTGGAGGTAG AGACCCAGAA ACTAGCTTAT 
ATGGAGCTAC AGCAGAAAGC TGAGTTCTCA GATCAGAAAC ATCAGAAGGA AATAGAAAAT 
ATGTGTTTGA AGACTTCTCA GCTTACTGGG CAAGTTGAAG ATCTAGAACA CAAGCTTCAG 
TTACTGTCAA ATGAAATAAT GGACAAAGAC CGGTGTTACC AAGACTTGCA TGCCGAATAT 
GAGAGCCTCA GGGATCTGCT AAAATCCAAA GATGCTTCTC TGGTGACAAA TGAAGATCAT 
CAGAGAAGTC TTTTGGCTTT TGATCAGCAG CCTGCCATGC ATCATTCCTT TGCAAATATA 
ATTGGAGAAC AAGGAAGCAT GCCTTCAGAG AGGAGTGAAT GTCGTTTAGA AGCAGACCAA 
AGTCCGAAAA ATTCTGCCAT CCTACAAAAT AGAGTTGATT CACTTGAATT TTCATTAGAG 
TCTCAAAAAC AGATGAACTC AGACCTGCAA AAGCAGTGTG AAGAGTTGGT GCAAATCAAA 
GGAGAAATAG AAGAAAATCT CATGAAAGCA GAACAGATGC ATCAAAGTTT TGTGGCTGAA 
ACAAGTCAGC GCATTAGTAA GTTACAGGAA GACACTTCTG CTCACCAGAA TGTTGTTGCT 
GAAACCTTAA GTGCCCTTGA GAACAAGGAA AAAGAGCTGC AACTTTTAAA TGATAAGGTA 
GAAACTGAGC AGGCAGAGAT TCAAGAATTA AAAAAGAGCA ACCATCTACT TGAAGACTCT 
CTAAAGGAGC TACAACTTTT ATCCGAAACC CTAAGCTTGG AGAAGAAAGA AATGAGTTCC 
ATCATTTCTC TAAATAAAAG GGAAATTGAA GAGCTGACCC AAGAGAATGG GACTCTTAAG 
GAAATTAATG CATCCTTAAA TCAAGAGAAG ATGAACTTAA TCCAGAAAAG TGAGAGTTTT 
GCAAACTATA TAGATGAAAG GGAGAAAAGC ATTTCAGAGT TATCTGATCA GTACAAGCAA 
GAAAAACTTA TTTTACTACA AAGATGTGAA GAAACCGGAA ATGCATATGA GGATCTTAGT 
CAAAAATACA AAGCAGCACA GGAAAAGAAT TCTAAATTAG AATGCTTGCT AAATGAATGC 
ACTAGTCTTT GTGAAAATAG GAAAAATGAG TTGGAACAGC TAAAGGAAGC ATTTGCAAAG 
GAACACCAAG AATTCTTAAC AAAATTAGCA TTTGCTGAAG AAAGAAATCA GAATCTGATG 
CTAGAGTTGG AGACAGTGCA GCAAGCTCTG AGATCTGAGA TGACAGATAA CCAAAACAAT 
TCTAAGAGCG AGGCTGGTGG TTTAAAGCAA GAAATCATGA CTTTAAAGGA AGAACAAAAC 
AAAATGCAAA AGGAAGTTAA TGACTTATTA CAAGAGAATG AACAGCTGAT GAAGGTAATG 
AAGACTAAAC ATGAATGTCA AAATCTAGAA TCAGAACCAA TTAGGAACTC TGTGAAAGAA 
AGAGAGAGTG AGAGAAATCA ATGTAATTTT AAACCTCAGA TGGATCTTGA AGTTAAAGAA 
ATTTCTCTAG ATAGTTATAA TGCGCAGTTG GTGCAATTAG AAGCTATGCT AAGAAATAAG 
GAATTAAAAC TTCAGGAAAG TGAGAAGGAG AAGGAGTGCC TGCAGCATGA ATTACAGACA 
ATTAGAGGAG ATCTTGAAAC CAGCAATTTG CAAGACATGC AGTCACAAGA AATTAGTGGC 
CTTAAAGACT GTGAAATAGA TGCGGAAGAA AAGTATATTT CAGGGCCTCA TGAGTTGTCA 
ACAAGTCAAA ACGACAATGC ACACCTTCAG TGCTCTCTGC AAACAACAAT GAACAAGCTG 
AATGAGCTAG AGAAAATATG TGAAATACTG CAGGCTGAAA AGTATGAACT CGTAACTGAG 
CTGAATGATT CAAGGTCAGA ATGTATCACA GCAACTAGGA AAATGGCAGA AGAGGTAGGG 
AAACTACTAA ATGAAGTTAA AATATTAAAT GATGACAGTG GTCTTCTCCA TGGTGAGTTA 
GTGGAAGACA TACCAGGAGG TGAATTTGGT GAACAACCAA ATGAACAGCA CCCTGTGTCT 
TTGGCTCCAT TGGACGAGAG TAATTCCTAC GAGCACTTGA CATTGTCAGA CAAAGAAGTT 
CAAATGCACT TTGCCGAATT GCAAGAGAAA TTCTTATCTT TACAAAGTGA ACACAAAATT 
TTACATGATC AGCACTGTCA GATGAGCTCT AAAATGTCAG AGCTGCAGAC CTATGTTGAC 
TCATTAAAGG CCGAAAATTT GGTCTTGTCA ACGAATCTGA GAAACTTTCA AGGTGACTTG 
GTGAAGGAGA TGCAGCTGGG CTTGGAGGAG GGGCTCGTTC CATCCCTGTC ATCCTCTTGT 
GTGCCTGACA GCTCTAGTCT TAGCAGTTTG GGAGACTCCT CCTTTTACAG AGCTCTTTTA 
GAACAGACAG GAGATATGTC TCTTTTGAGT AATTTAGAAG GGGCTGTTTC AGCAAACCAG 
TGCAGTGTAG ATGAAGTATT TTGCAGCAGT CTGCAGGAGG AGAATCTGAC CAGGAAAGAA 
ACCCCTTCGG CCCCAGCGAA GGGTGTTGAA GAGCTTGAGT CCCTCTGTGA GGTGTACCGG 
CAGTCCCTCG AGAAGCTAGA AGAGAAAATG GAAAGTCAAG GGATTATGAA AAATAAGGAA 
ATTCAAGAGC TCGAGCAGTT ATTAAGTTCT GAAAGGCAAG AGCTTGACTG CCTTAGGAAG 
CAGTATTTGT CAGAAAATGA ACAGTGGCAA CAGAAGCTGA CAAGCGTGAC TCTGGAGATG 
GAGTCCAAGT TGGCGGCAGA AAAGAAACAG ACGGAACAAC TGTCACTTGA GCTGGAAGTA 
GCACGACTCC AGCTACAAGG TCTGGACTTA AGTTCTCGGT CTTTGCTTGG CATCGACACA 
GAAGATGCTA TTCAAGGCCG AAATGAGAGC TGTGACATAT CAAAAGAACA TACTTCAGAA 
ACTACAGAAA GAACACCAAA GCATGATGTT CATCAGATTT GTGATAAAGA TGCTCAGCAG 
GACCTCAATC TAGACATTGA GAAAATAACT GAGACTGGTG CAGTGAAACC CACAGGAGAG 
TGCTCTGGGG AACAGTCCCC AGATACCAAT TATGAGCCTC CAGGGGAAGA TAAAACCCAG 
GGCTCTTCAG AATGCATTTC TGAATTGTCA TTTTCTGGTC CTAATGCTTT GGTACCTATG 
GATTTCCTGG GGAATCAGGA AGATATCCAT AATCTTCAAC TGCGGGTAAA AGAGACATCA 
AATGAGAATT TGAGATTACT TCATGTGATA GAGGACCGTG ACAGAAAAGT TGAAAGTTTG 
CTAAATGAAA TGAAAGAATT AGACTCAAAA CTCCATTTAC AGGAGGTACA ACTAATGACC 
AAAATTGAAG CATGCATAGA ATTGGAAAAA ATAGTTGGGG AACTTAAGAA AGAAAACTCA 
GATTTAAGTG AAAAATTGGA ATATTTTTCT TGTGATCACC AGGAGTTACT CCAGAGAGTA 
GAAACTTCTG AAGGCCTCAA TTCTGATTTA GAAATGCATG CAGATAAATC ATCACGTGAA 
GATATTGGAG ATAATGTGGC CAAGGTGAAT GACAGCTGGA AGGAGAGATT TCTTGATGTG 
GAAAATGAGC TGAGTAGGAT CAGATCGGAG AAAGCTAGCA TTGAGCATGA AGCCCTCTAC 
CTGGAGGCTG ACTTAGAGGT AGTTCAAACA GAGAAGCTAT GTTTAGAAAA AGACAATGAA 
AATAAGCAGA AGGTTATTGT CTGCCTTGAA GAAGAACTCT CAGTGGTCAC AAGTGAGAGA 
AACCAGCTTC GTGGAGAATT AGATACTATG TCAAAAAAAA CCACGGCACT GGATCAGTTG 
TCTGAAAAAA TGAAGGAGAA AACACAAGAG CTTGAGTCTC ATCAAAGTGA GTGTCTCCAT 
TGCATTCAGG TGGCAGAGGC AGAGGTGAAG GAAAAGACGG AACTCCTTCA GACTTTGTCC 
TCTGATGTGA GTGAGCTGTT AAAAGACAAA ACTCATCTCC AGGAAAAGCT GCAGAGTTTG 
GAAAAGGACT CACAGGCACT GTCTTTGACA AAATGTGAGC TGGAAAACCA AATTGCACAA 
CTGAATAAAG AGAAAGAATT GCTTGTCAAG GAATCTGAAA GCCTGCAGGC CAGACTGAGT 
GAATCAGATT ATGAAAAGCT GAATGTCTCC AAGGCCTTGG AGGCCGCACT GGTGGAGAAA 
GGTGAGTTCG CATTGAGGCT GAGCTCAACA CAGGAGGAAG TGCATCAGCT GAGAAGAGGC 
ATCGAGAAAC TGAGAGTTCG CATTGAGGCC GATGAAAAGA AGCAGCTGCA CATCGCAGAG 
AAACTGAAAG AACGCGAGCG GGAGAATGAT TCACTTAAGG ATAAAGTTGA GAACCTTGAA 
AGGGAATTGC AGATGTCAGA AGAAAACCAG GAGCTAGTGA TTCTTGATGC CGAGAATTCC 
AAAGCAGAAG TAGAGACTCT AAAAACACAA ATAGAAGAGA TGGCCAGAAG CCTGAAAGTT 
TTTGAATTAG ACCTTGTCAC GTTAAGGTCT GAAAAAGAAA ATCTGACAAA ACAAATACAA 
GAAAAACAAG GTCAGTTGTC AGAACTAGAC AAGTTACTCT CTTCATTTAA AAGTCTGTTA 
GAAGAAAAGG AGCAAGCAGA GATACAGATC AAAGAAGAAT CTAAAACTGC AGTGGAGATG 
CTTCAGAATC AGTTAAAGGA GCTAAATGAG GCAGTAGCAG CCTTGTGTGG TGACCAAGAA 
ATTATGAAGG CCACAGAACA GAGTCTAGAC CCACCAATAG AGGAAGAGCA TCAGCTGAGA 
AATAGCATTG AAAAGCTGAG AGCCCGCCTA GAAGCTGATG AAAAGAAGCA GCTCTGTGTC 
TTACAACAAC TGAAGGAAAG TGAGCATCAT GCAGATTTAC TTAAGGGTAG AGTGGAGAAC 
CTTGAAAGAG AGCTAGAGAT AGCCAGGACA AACCAAGAGC ATGCAGCTCT TGAGGCAGAG 
AATTCCAAAG GAGAGGTAGA GACCCTAAAA GCAAAAATAG AAGGGATGAC CCAAAGTCTG 
AGAGGTCTGG AATTAGATGT TGTTACTATA AGGTCAGAAA AAGAAAATCT GACAAATGAA 
TTACAAAAAG AGCAAGAGCG AATATCTGAA TTAGAAATAA TAAATTCATC ATTTGAAAAT 
ATTTTGCAAG AAAAAGAGCA AGAGAAAGTA CAGATGAAAG AAAAATCAAG CACTGCCATG 
GAGATGCTTC AAACACAATT AAAAGAGCTC AATGAGAGAG TGGCAGCCCT GCATAATGAC 
CAAGAAGCCT GTAAGGCCAA AGAGCAGAAT CTTAGTAGTC AAGTAGAGTG TCTTGAACTT 
GAGAAGGCTC AGTTGCTACA AGGCCTTGAT GAGGCCAAAA ATAATTATAT TGTTTTGCAA 
TCTTCAGTGA ATGGCCTCAT TCAAGAAGTA GAAGATGGCA AGCAGAAACT GGAGAAGAAG 
GATGAAGAAA TCAGTAGACT GAAAAATCAA ATTCAAGACC AAGAGCAGCT TGTCTCTAAA 
CTGTCCCAGG TGGAAGGAGA GCACCAACTT TGGAAGGAGC AAAACTTAGA ACTGAGAAAT 
CTGACAGTGG AATTGGAGCA GAAGATCCAA GTGCTACAAT CCAAAAATGC CTCTTTGCAG 
GACACATTAG AAGTGCTGCA GAGTTCTTAC AAGAATCTAG AGAATGAGCT TGAATTGACA 
AAAATGGACA AAATGTCCTT TGTTGAAAAA GTAAACAAAA TGACTGCAAA GGAAACTGAG 
CTGCAGAGGG AAATGCATGA GATGGCACAG AAAACAGCAG AGCTGCAAGA AGAACTCAGT 
GGAGAGAAAA ATAGGCTAGC TGGAGAGTTG CAGTTACTGT TGGAAGAAAT AAAGAGCAGC 
AAATATGAAG TAGAAATCCA GACATACCGA GAGAAATTGA CTTCTAAAGA AGAATGTCTC 
AGTTCACAGA AGCTGGAGAT AGACCTTTTA AAGTCTAGTA AAGAAGAGCT CAATAATTCA 
TTGAAAGCTA CTACTCAGAT TTTGGAAGAA TTGAAGAAAA CCAAGATGGA CAATCTAAAA 
TATGTAAATC AGTTGAAGAA GGAAAATGAA CGTGCCCAGG GGAAAATGAA GTTGTTGATC 
AAATCCTGTA AACAGCTGGA AGAGGAAAAG GAGATACTGC AGAAAGAACT CTCTCAACTT 
CAAGCTGCAC AGGAGAAGCA GAAAACAGGT ACTGTTATGG ATACCAAGGT CGATGAATTA 
ACAACTGAGA TCAAAGAACT GAAAGAAACT CTTGAAGAAA AAACCAAGGA GGCAGATGAA 
TACTTGGATA AGTACTGTTC CTTGCTTATA AGCCATGAAA AGTTAGAGAA AGCTAAAGAG 
ATGTTAGAGA CACAAGTGGC CCATCTGTGT TCACAGCAAT CTAAACAAGA TTCCCGAGGG 
TCTCCTTTGC TAGGTCCAGT TGTTCCAGGA CCATCTCCAA TCCCTTCTGT TACTGAAAAG 
AGGTTATCAT CTGGCCAAAA TAAAGCTTCA GGCAAGAGGC AAAGATCCAG TGGAATATGG 
GAGAATGGTA GAGGACCAAC ACCTGCTACC CCAGAGAGCT TTTCTAAAAA AAGCAAGAAA 
GCAGTCATGA GTGGTATTCA CCCTGCAGAA GACACGGAAG GTACTGAGTT TGAGCCAGAG 
GGACTTCCAG AAGTTGTAAA GAAAGGGTTT GCTGACATCC CGACAGGAAA GACTAGCCCA 
TATATCCTGC GAAGAACAAC CATGGCAACT CGGACCAGCC CCCGCCTGGC TGCACAGAAG 
TTAGCGCTAT CCCCACTGAG TCTCGGCAAA GAAAATCTTG CAGAGTCCTC CAAACCAACA 
GCTGGTGGCA GCAGATCACA AAAGGTCAAA GTTGCTCAGC GGAGCCCAGT AGATTCAGGC 
ACCATCCTCC GAGAACCCAC CACGAAATCC GTCCCAGTCA ATAATCTTCC TGAGAGAAGT 
CCGACTGACA GCCCCAGAGA GGGCCTGAGG GTCAAGCGAG GCCGACTTGT CCCCAGCCCC 
AAAGCTGGAC TGGAGTCCAA CGGCAGTGAG AACTGTAAGG TCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011507384.1
CDS	183..9350
Translation	MSWALEEWKEGLPTRALQKIQELEGQLDKLKKEKQQRQFQLDSLEAALQKQKQKVENEKTEGTNLKRENQRLMEICESLEKTKQKISHELQVKESQVNFQEGQLNSGKKQIEKLEQELKRCKSELERSQQAAQSADVSLNPCNTPQKIFTTPLTPSQYYSGSKYEDLKEKYNKEVEERKRLEAEVKALQAKKASQTLPQATMNHRDIARHQASSSVFSWQQEKTPSHLSSNSQRTPIRRDFSASYFSGEQEVTPSRSTLQIGKRDANSSFFDNSSSPHLLDQLKAQNQELRNKINELELRLQGHEKEMKGQVNKFQELQLQLEKAKVELIEKEKVLNKCRDELVRTTAQYDQASTKYTALEQKLKKLTEDLSCQRQNAESARCSLEQKIKEKEKEFQEELSRQQRSFQTLDQECIQMKARLTQELQQAKNMHNVLQAELDKLTSVKQQLENNLEEFKQKLCRAEQAFQASQIKENELRRSMEEMKKENNLLKSHSEQKAREVCHLEAELKNIKQCLNQSQNFAEEMKAKNTSQETMLRDLQEKINQQENSLTLEKLKLAVADLEKQRDCSQDLLKKREHHIEQLNDKLSKTEKESKALLSALELKKKEYEELKEEKTLFSCWKSENEKLLTQMESEKENLQSKINHLETCLKTQQIKSHEYNERVRTLEMDRENLSVEIRNLHNVLDSKSVEVETQKLAYMELQQKAEFSDQKHQKEIENMCLKTSQLTGQVEDLEHKLQLLSNEIMDKDRCYQDLHAEYESLRDLLKSKDASLVTNEDHQRSLLAFDQQPAMHHSFANIIGEQGSMPSERSECRLEADQSPKNSAILQNRVDSLEFSLESQKQMNSDLQKQCEELVQIKGEIEENLMKAEQMHQSFVAETSQRISKLQEDTSAHQNVVAETLSALENKEKELQLLNDKVETEQAEIQELKKSNHLLEDSLKELQLLSETLSLEKKEMSSIISLNKREIEELTQENGTLKEINASLNQEKMNLIQKSESFANYIDEREKSISELSDQYKQEKLILLQRCEETGNAYEDLSQKYKAAQEKNSKLECLLNECTSLCENRKNELEQLKEAFAKEHQEFLTKLAFAEERNQNLMLELETVQQALRSEMTDNQNNSKSEAGGLKQEIMTLKEEQNKMQKEVNDLLQENEQLMKVMKTKHECQNLESEPIRNSVKERESERNQCNFKPQMDLEVKEISLDSYNAQLVQLEAMLRNKELKLQESEKEKECLQHELQTIRGDLETSNLQDMQSQEISGLKDCEIDAEEKYISGPHELSTSQNDNAHLQCSLQTTMNKLNELEKICEILQAEKYELVTELNDSRSECITATRKMAEEVGKLLNEVKILNDDSGLLHGELVEDIPGGEFGEQPNEQHPVSLAPLDESNSYEHLTLSDKEVQMHFAELQEKFLSLQSEHKILHDQHCQMSSKMSELQTYVDSLKAENLVLSTNLRNFQGDLVKEMQLGLEEGLVPSLSSSCVPDSSSLSSLGDSSFYRALLEQTGDMSLLSNLEGAVSANQCSVDEVFCSSLQEENLTRKETPSAPAKGVEELESLCEVYRQSLEKLEEKMESQGIMKNKEIQELEQLLSSERQELDCLRKQYLSENEQWQQKLTSVTLEMESKLAAEKKQTEQLSLELEVARLQLQGLDLSSRSLLGIDTEDAIQGRNESCDISKEHTSETTERTPKHDVHQICDKDAQQDLNLDIEKITETGAVKPTGECSGEQSPDTNYEPPGEDKTQGSSECISELSFSGPNALVPMDFLGNQEDIHNLQLRVKETSNENLRLLHVIEDRDRKVESLLNEMKELDSKLHLQEVQLMTKIEACIELEKIVGELKKENSDLSEKLEYFSCDHQELLQRVETSEGLNSDLEMHADKSSREDIGDNVAKVNDSWKERFLDVENELSRIRSEKASIEHEALYLEADLEVVQTEKLCLEKDNENKQKVIVCLEEELSVVTSERNQLRGELDTMSKKTTALDQLSEKMKEKTQELESHQSECLHCIQVAEAEVKEKTELLQTLSSDVSELLKDKTHLQEKLQSLEKDSQALSLTKCELENQIAQLNKEKELLVKESESLQARLSESDYEKLNVSKALEAALVEKGEFALRLSSTQEEVHQLRRGIEKLRVRIEADEKKQLHIAEKLKERERENDSLKDKVENLERELQMSEENQELVILDAENSKAEVETLKTQIEEMARSLKVFELDLVTLRSEKENLTKQIQEKQGQLSELDKLLSSFKSLLEEKEQAEIQIKEESKTAVEMLQNQLKELNEAVAALCGDQEIMKATEQSLDPPIEEEHQLRNSIEKLRARLEADEKKQLCVLQQLKESEHHADLLKGRVENLERELEIARTNQEHAALEAENSKGEVETLKAKIEGMTQSLRGLELDVVTIRSEKENLTNELQKEQERISELEIINSSFENILQEKEQEKVQMKEKSSTAMEMLQTQLKELNERVAALHNDQEACKAKEQNLSSQVECLELEKAQLLQGLDEAKNNYIVLQSSVNGLIQEVEDGKQKLEKKDEEISRLKNQIQDQEQLVSKLSQVEGEHQLWKEQNLELRNLTVELEQKIQVLQSKNASLQDTLEVLQSSYKNLENELELTKMDKMSFVEKVNKMTAKETELQREMHEMAQKTAELQEELSGEKNRLAGELQLLLEEIKSSKYEVEIQTYREKLTSKEECLSSQKLEIDLLKSSKEELNNSLKATTQILEELKKTKMDNLKYVNQLKKENERAQGKMKLLIKSCKQLEEEKEILQKELSQLQAAQEKQKTGTVMDTKVDELTTEIKELKETLEEKTKEADEYLDKYCSLLISHEKLEKAKEMLETQVAHLCSQQSKQDSRGSPLLGPVVPGPSPIPSVTEKRLSSGQNKASGKRQRSSGIWENGRGPTPATPESFSKKSKKAVMSGIHPAEDTEGTEFEPEGLPEVVKKGFADIPTGKTSPYILRRTTMATRTSPRLAAQKLALSPLSLGKENLAESSKPTAGGSRSQKVKVAQRSPVDSGTILREPTTKSVPVNNLPERSPTDSPREGLRVKRGRLVPSPKAGLESNGSENCKVQ

Target ORF information:

RefSeq Version	XM_011509082.2
Organism	Homo sapiens(human)
Definition	Homo sapiens centromere protein F (CENPF), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011509082.2

ORF Insert Sequence:

ATGAGCTGGG CTTTGGAAGA ATGGAAAGAA GGGCTGCCTA CAAGAGCTCT TCAGAAAATT 
CAAGAGCTTG AAGGACAGCT TGACAAACTG AAGAAGGAAA AGCAGCAAAG GCAGTTTCAG 
CTTGACAGTC TCGAGGCTGC GCTGCAGAAG CAAAAACAGA AGGTTGAAAA TGAAAAAACC 
GAGGGTACAA ACCTGAAAAG GGAGAATCAA AGATTGATGG AAATATGTGA AAGTCTGGAG 
AAAACTAAGC AGAAGATTTC TCATGAACTT CAAGTCAAGG AGTCACAAGT GAATTTCCAG 
GAAGGACAAC TGAATTCAGG CAAAAAACAA ATAGAAAAAC TGGAACAGGA ACTTAAAAGG 
TGTAAATCTG AGCTTGAAAG AAGCCAACAA GCTGCGCAGT CTGCAGATGT CTCTCTGAAT 
CCATGCAATA CACCACAAAA AATTTTTACA ACTCCACTAA CACCAAGTCA ATATTATAGT 
GGTTCCAAGT ATGAAGATCT AAAAGAAAAA TATAATAAAG AGGTTGAAGA ACGAAAAAGA 
TTAGAGGCAG AGGTTAAAGC CTTGCAGGCT AAAAAAGCAA GCCAGACTCT TCCACAAGCC 
ACCATGAATC ACCGCGACAT TGCCCGGCAT CAGGCTTCAT CATCTGTGTT CTCATGGCAG 
CAAGAGAAGA CCCCAAGTCA TCTTTCATCT AATTCTCAAA GAACTCCAAT TAGGAGAGAT 
TTCTCTGCAT CTTACTTTTC TGGGGAACAA GAGGTGACTC CAAGTCGATC AACTTTGCAA 
ATAGGGAAAA GAGATGCTAA TAGCAGTTTC TTTGACAATT CTAGCAGTCC TCATCTTTTG 
GATCAATTAA AAGCGCAGAA TCAAGAGCTA AGAAACAAGA TTAATGAGTT GGAACTACGC 
CTGCAAGGAC ATGAAAAAGA AATGAAAGGC CAAGTGAATA AGTTTCAAGA ACTCCAACTC 
CAACTGGAGA AAGCAAAAGT GGAATTAATT GAAAAAGAGA AAGTTTTGAA CAAATGTAGG 
GATGAACTAG TGAGAACAAC AGCACAATAC GACCAGGCGT CAACCAAGTA TACTGCATTG 
GAACAAAAAC TGAAAAAATT GACGGAAGAT TTGAGTTGTC AGCGACAAAA TGCAGAAAGT 
GCCAGATGTT CTCTGGAACA GAAAATTAAG GAAAAAGAAA AGGAGTTTCA AGAGGAGCTC 
TCCCGTCAAC AGCGTTCTTT CCAAACACTG GACCAGGAGT GCATCCAGAT GAAGGCCAGA 
CTCACCCAGG AGTTACAGCA AGCCAAGAAT ATGCACAACG TCCTGCAGGC TGAACTGGAT 
AAACTCACAT CAGTAAAGCA ACAGCTAGAA AACAATTTGG AAGAGTTTAA GCAAAAGTTG 
TGCAGAGCTG AACAGGCGTT CCAGGCGAGT CAGATCAAGG AGAATGAGCT GAGGAGAAGC 
ATGGAGGAAA TGAAGAAGGA AAACAACCTC CTTAAGAGTC ACTCTGAGCA AAAGGCCAGA 
GAAGTCTGCC ACCTGGAGGC AGAACTCAAG AACATCAAAC AGTGTTTAAA TCAGAGCCAG 
AATTTTGCAG AAGAAATGAA AGCGAAGAAT ACCTCTCAGG AAACCATGTT AAGAGATCTT 
CAAGAAAAAA TAAATCAGCA AGAAAACTCC TTGACTTTAG AAAAACTGAA GCTTGCTGTG 
GCTGATCTGG AAAAGCAGCG AGATTGTTCT CAAGACCTTT TGAAGAAAAG AGAACATCAC 
ATTGAACAAC TTAATGATAA GTTAAGCAAG ACAGAGAAAG AGTCCAAAGC CTTGCTGAGT 
GCTTTAGAGT TAAAAAAGAA AGAATATGAA GAATTGAAAG AAGAGAAAAC TCTGTTTTCT 
TGTTGGAAAA GTGAAAACGA AAAACTTTTA ACTCAGATGG AATCAGAAAA GGAAAACTTG 
CAGAGTAAAA TTAATCACTT GGAAACTTGT CTGAAGACAC AGCAAATAAA AAGTCATGAA 
TACAACGAGA GAGTAAGAAC GCTGGAGATG GACAGAGAAA ACCTAAGTGT CGAGATCAGA 
AACCTTCACA ACGTGTTAGA CAGTAAGTCA GTGGAGGTAG AGACCCAGAA ACTAGCTTAT 
ATGGAGCTAC AGCAGAAAGC TGAGTTCTCA GATCAGAAAC ATCAGAAGGA AATAGAAAAT 
ATGTGTTTGA AGACTTCTCA GCTTACTGGG CAAGTTGAAG ATCTAGAACA CAAGCTTCAG 
TTACTGTCAA ATGAAATAAT GGACAAAGAC CGGTGTTACC AAGACTTGCA TGCCGAATAT 
GAGAGCCTCA GGGATCTGCT AAAATCCAAA GATGCTTCTC TGGTGACAAA TGAAGATCAT 
CAGAGAAGTC TTTTGGCTTT TGATCAGCAG CCTGCCATGC ATCATTCCTT TGCAAATATA 
ATTGGAGAAC AAGGAAGCAT GCCTTCAGAG AGGAGTGAAT GTCGTTTAGA AGCAGACCAA 
AGTCCGAAAA ATTCTGCCAT CCTACAAAAT AGAGTTGATT CACTTGAATT TTCATTAGAG 
TCTCAAAAAC AGATGAACTC AGACCTGCAA AAGCAGTGTG AAGAGTTGGT GCAAATCAAA 
GGAGAAATAG AAGAAAATCT CATGAAAGCA GAACAGATGC ATCAAAGTTT TGTGGCTGAA 
ACAAGTCAGC GCATTAGTAA GTTACAGGAA GACACTTCTG CTCACCAGAA TGTTGTTGCT 
GAAACCTTAA GTGCCCTTGA GAACAAGGAA AAAGAGCTGC AACTTTTAAA TGATAAGGTA 
GAAACTGAGC AGGCAGAGAT TCAAGAATTA AAAAAGAGCA ACCATCTACT TGAAGACTCT 
CTAAAGGAGC TACAACTTTT ATCCGAAACC CTAAGCTTGG AGAAGAAAGA AATGAGTTCC 
ATCATTTCTC TAAATAAAAG GGAAATTGAA GAGCTGACCC AAGAGAATGG GACTCTTAAG 
GAAATTAATG CATCCTTAAA TCAAGAGAAG ATGAACTTAA TCCAGAAAAG TGAGAGTTTT 
GCAAACTATA TAGATGAAAG GGAGAAAAGC ATTTCAGAGT TATCTGATCA GTACAAGCAA 
GAAAAACTTA TTTTACTACA AAGATGTGAA GAAACCGGAA ATGCATATGA GGATCTTAGT 
CAAAAATACA AAGCAGCACA GGAAAAGAAT TCTAAATTAG AATGCTTGCT AAATGAATGC 
ACTAGTCTTT GTGAAAATAG GAAAAATGAG TTGGAACAGC TAAAGGAAGC ATTTGCAAAG 
GAACACCAAG AATTCTTAAC AAAATTAGCA TTTGCTGAAG AAAGAAATCA GAATCTGATG 
CTAGAGTTGG AGACAGTGCA GCAAGCTCTG AGATCTGAGA TGACAGATAA CCAAAACAAT 
TCTAAGAGCG AGGCTGGTGG TTTAAAGCAA GAAATCATGA CTTTAAAGGA AGAACAAAAC 
AAAATGCAAA AGGAAGTTAA TGACTTATTA CAAGAGAATG AACAGCTGAT GAAGGTAATG 
AAGACTAAAC ATGAATGTCA AAATCTAGAA TCAGAACCAA TTAGGAACTC TGTGAAAGAA 
AGAGAGAGTG AGAGAAATCA ATGTAATTTT AAACCTCAGA TGGATCTTGA AGTTAAAGAA 
ATTTCTCTAG ATAGTTATAA TGCGCAGTTG GTGCAATTAG AAGCTATGCT AAGAAATAAG 
GAATTAAAAC TTCAGGAAAG TGAGAAGGAG AAGGAGTGCC TGCAGCATGA ATTACAGACA 
ATTAGAGGAG ATCTTGAAAC CAGCAATTTG CAAGACATGC AGTCACAAGA AATTAGTGGC 
CTTAAAGACT GTGAAATAGA TGCGGAAGAA AAGTATATTT CAGGGCCTCA TGAGTTGTCA 
ACAAGTCAAA ACGACAATGC ACACCTTCAG TGCTCTCTGC AAACAACAAT GAACAAGCTG 
AATGAGCTAG AGAAAATATG TGAAATACTG CAGGCTGAAA AGTATGAACT CGTAACTGAG 
CTGAATGATT CAAGGTCAGA ATGTATCACA GCAACTAGGA AAATGGCAGA AGAGGTAGGG 
AAACTACTAA ATGAAGTTAA AATATTAAAT GATGACAGTG GTCTTCTCCA TGGTGAGTTA 
GTGGAAGACA TACCAGGAGG TGAATTTGGT GAACAACCAA ATGAACAGCA CCCTGTGTCT 
TTGGCTCCAT TGGACGAGAG TAATTCCTAC GAGCACTTGA CATTGTCAGA CAAAGAAGTT 
CAAATGCACT TTGCCGAATT GCAAGAGAAA TTCTTATCTT TACAAAGTGA ACACAAAATT 
TTACATGATC AGCACTGTCA GATGAGCTCT AAAATGTCAG AGCTGCAGAC CTATGTTGAC 
TCATTAAAGG CCGAAAATTT GGTCTTGTCA ACGAATCTGA GAAACTTTCA AGGTGACTTG 
GTGAAGGAGA TGCAGCTGGG CTTGGAGGAG GGGCTCGTTC CATCCCTGTC ATCCTCTTGT 
GTGCCTGACA GCTCTAGTCT TAGCAGTTTG GGAGACTCCT CCTTTTACAG AGCTCTTTTA 
GAACAGACAG GAGATATGTC TCTTTTGAGT AATTTAGAAG GGGCTGTTTC AGCAAACCAG 
TGCAGTGTAG ATGAAGTATT TTGCAGCAGT CTGCAGGAGG AGAATCTGAC CAGGAAAGAA 
ACCCCTTCGG CCCCAGCGAA GGGTGTTGAA GAGCTTGAGT CCCTCTGTGA GGTGTACCGG 
CAGTCCCTCG AGAAGCTAGA AGAGAAAATG GAAAGTCAAG GGATTATGAA AAATAAGGAA 
ATTCAAGAGC TCGAGCAGTT ATTAAGTTCT GAAAGGCAAG AGCTTGACTG CCTTAGGAAG 
CAGTATTTGT CAGAAAATGA ACAGTGGCAA CAGAAGCTGA CAAGCGTGAC TCTGGAGATG 
GAGTCCAAGT TGGCGGCAGA AAAGAAACAG ACGGAACAAC TGTCACTTGA GCTGGAAGTA 
GCACGACTCC AGCTACAAGG TCTGGACTTA AGTTCTCGGT CTTTGCTTGG CATCGACACA 
GAAGATGCTA TTCAAGGCCG AAATGAGAGC TGTGACATAT CAAAAGAACA TACTTCAGAA 
ACTACAGAAA GAACACCAAA GCATGATGTT CATCAGATTT GTGATAAAGA TGCTCAGCAG 
GACCTCAATC TAGACATTGA GAAAATAACT GAGACTGGTG CAGTGAAACC CACAGGAGAG 
TGCTCTGGGG AACAGTCCCC AGATACCAAT TATGAGCCTC CAGGGGAAGA TAAAACCCAG 
GGCTCTTCAG AATGCATTTC TGAATTGTCA TTTTCTGGTC CTAATGCTTT GGTACCTATG 
GATTTCCTGG GGAATCAGGA AGATATCCAT AATCTTCAAC TGCGGGTAAA AGAGACATCA 
AATGAGAATT TGAGATTACT TCATGTGATA GAGGACCGTG ACAGAAAAGT TGAAAGTTTG 
CTAAATGAAA TGAAAGAATT AGACTCAAAA CTCCATTTAC AGGAGGTACA ACTAATGACC 
AAAATTGAAG CATGCATAGA ATTGGAAAAA ATAGTTGGGG AACTTAAGAA AGAAAACTCA 
GATTTAAGTG AAAAATTGGA ATATTTTTCT TGTGATCACC AGGAGTTACT CCAGAGAGTA 
GAAACTTCTG AAGGCCTCAA TTCTGATTTA GAAATGCATG CAGATAAATC ATCACGTGAA 
GATATTGGAG ATAATGTGGC CAAGGTGAAT GACAGCTGGA AGGAGAGATT TCTTGATGTG 
GAAAATGAGC TGAGTAGGAT CAGATCGGAG AAAGCTAGCA TTGAGCATGA AGCCCTCTAC 
CTGGAGGCTG ACTTAGAGGT AGTTCAAACA GAGAAGCTAT GTTTAGAAAA AGACAATGAA 
AATAAGCAGA AGGTTATTGT CTGCCTTGAA GAAGAACTCT CAGTGGTCAC AAGTGAGAGA 
AACCAGCTTC GTGGAGAATT AGATACTATG TCAAAAAAAA CCACGGCACT GGATCAGTTG 
TCTGAAAAAA TGAAGGAGAA AACACAAGAG CTTGAGTCTC ATCAAAGTGA GTGTCTCCAT 
TGCATTCAGG TGGCAGAGGC AGAGGTGAAG GAAAAGACGG AACTCCTTCA GACTTTGTCC 
TCTGATGTGA GTGAGCTGTT AAAAGACAAA ACTCATCTCC AGGAAAAGCT GCAGAGTTTG 
GAAAAGGACT CACAGGCACT GTCTTTGACA AAATGTGAGC TGGAAAACCA AATTGCACAA 
CTGAATAAAG AGAAAGAATT GCTTGTCAAG GAATCTGAAA GCCTGCAGGC CAGACTGAGT 
GAATCAGATT ATGAAAAGCT GAATGTCTCC AAGGCCTTGG AGGCCGCACT GGTGGAGAAA 
GGTGAGTTCG CATTGAGGCT GAGCTCAACA CAGGAGGAAG TGCATCAGCT GAGAAGAGGC 
ATCGAGAAAC TGAGAGTTCG CATTGAGGCC GATGAAAAGA AGCAGCTGCA CATCGCAGAG 
AAACTGAAAG AACGCGAGCG GGAGAATGAT TCACTTAAGG ATAAAGTTGA GAACCTTGAA 
AGGGAATTGC AGATGTCAGA AGAAAACCAG GAGCTAGTGA TTCTTGATGC CGAGAATTCC 
AAAGCAGAAG TAGAGACTCT AAAAACACAA ATAGAAGAGA TGGCCAGAAG CCTGAAAGTT 
TTTGAATTAG ACCTTGTCAC GTTAAGGTCT GAAAAAGAAA ATCTGACAAA ACAAATACAA 
GAAAAACAAG GTCAGTTGTC AGAACTAGAC AAGTTACTCT CTTCATTTAA AAGTCTGTTA 
GAAGAAAAGG AGCAAGCAGA GATACAGATC AAAGAAGAAT CTAAAACTGC AGTGGAGATG 
CTTCAGAATC AGTTAAAGGA GCTAAATGAG GCAGTAGCAG CCTTGTGTGG TGACCAAGAA 
ATTATGAAGG CCACAGAACA GAGTCTAGAC CCACCAATAG AGGAAGAGCA TCAGCTGAGA 
AATAGCATTG AAAAGCTGAG AGCCCGCCTA GAAGCTGATG AAAAGAAGCA GCTCTGTGTC 
TTACAACAAC TGAAGGAAAG TGAGCATCAT GCAGATTTAC TTAAGGGTAG AGTGGAGAAC 
CTTGAAAGAG AGCTAGAGAT AGCCAGGACA AACCAAGAGC ATGCAGCTCT TGAGGCAGAG 
AATTCCAAAG GAGAGGTAGA GACCCTAAAA GCAAAAATAG AAGGGATGAC CCAAAGTCTG 
AGAGGTCTGG AATTAGATGT TGTTACTATA AGGTCAGAAA AAGAAAATCT GACAAATGAA 
TTACAAAAAG AGCAAGAGCG AATATCTGAA TTAGAAATAA TAAATTCATC ATTTGAAAAT 
ATTTTGCAAG AAAAAGAGCA AGAGAAAGTA CAGATGAAAG AAAAATCAAG CACTGCCATG 
GAGATGCTTC AAACACAATT AAAAGAGCTC AATGAGAGAG TGGCAGCCCT GCATAATGAC 
CAAGAAGCCT GTAAGGCCAA AGAGCAGAAT CTTAGTAGTC AAGTAGAGTG TCTTGAACTT 
GAGAAGGCTC AGTTGCTACA AGGCCTTGAT GAGGCCAAAA ATAATTATAT TGTTTTGCAA 
TCTTCAGTGA ATGGCCTCAT TCAAGAAGTA GAAGATGGCA AGCAGAAACT GGAGAAGAAG 
GATGAAGAAA TCAGTAGACT GAAAAATCAA ATTCAAGACC AAGAGCAGCT TGTCTCTAAA 
CTGTCCCAGG TGGAAGGAGA GCACCAACTT TGGAAGGAGC AAAACTTAGA ACTGAGAAAT 
CTGACAGTGG AATTGGAGCA GAAGATCCAA GTGCTACAAT CCAAAAATGC CTCTTTGCAG 
GACACATTAG AAGTGCTGCA GAGTTCTTAC AAGAATCTAG AGAATGAGCT TGAATTGACA 
AAAATGGACA AAATGTCCTT TGTTGAAAAA GTAAACAAAA TGACTGCAAA GGAAACTGAG 
CTGCAGAGGG AAATGCATGA GATGGCACAG AAAACAGCAG AGCTGCAAGA AGAACTCAGT 
GGAGAGAAAA ATAGGCTAGC TGGAGAGTTG CAGTTACTGT TGGAAGAAAT AAAGAGCAGC 
AAATATGAAG TAGAAATCCA GACATACCGA GAGAAATTGA CTTCTAAAGA AGAATGTCTC 
AGTTCACAGA AGCTGGAGAT AGACCTTTTA AAGTCTAGTA AAGAAGAGCT CAATAATTCA 
TTGAAAGCTA CTACTCAGAT TTTGGAAGAA TTGAAGAAAA CCAAGATGGA CAATCTAAAA 
TATGTAAATC AGTTGAAGAA GGAAAATGAA CGTGCCCAGG GGAAAATGAA GTTGTTGATC 
AAATCCTGTA AACAGCTGGA AGAGGAAAAG GAGATACTGC AGAAAGAACT CTCTCAACTT 
CAAGCTGCAC AGGAGAAGCA GAAAACAGGT ACTGTTATGG ATACCAAGGT CGATGAATTA 
ACAACTGAGA TCAAAGAACT GAAAGAAACT CTTGAAGAAA AAACCAAGGA GGCAGATGAA 
TACTTGGATA AGTACTGTTC CTTGCTTATA AGCCATGAAA AGTTAGAGAA AGCTAAAGAG 
ATGTTAGAGA CACAAGTGGC CCATCTGTGT TCACAGCAAT CTAAACAAGA TTCCCGAGGG 
TCTCCTTTGC TAGGTCCAGT TGTTCCAGGA CCATCTCCAA TCCCTTCTGT TACTGAAAAG 
AGGTTATCAT CTGGCCAAAA TAAAGCTTCA GGCAAGAGGC AAAGATCCAG TGGAATATGG 
GAGAATGGTA GAGGACCAAC ACCTGCTACC CCAGAGAGCT TTTCTAAAAA AAGCAAGAAA 
GCAGTCATGA GTGGTATTCA CCCTGCAGAA GACACGGAAG GTACTGAGTT TGAGCCAGAG 
GGACTTCCAG AAGTTGTAAA GAAAGGGTTT GCTGACATCC CGACAGGAAA GACTAGCCCA 
TATATCCTGC GAAGAACAAC CATGGCAACT CGGACCAGCC CCCGCCTGGC TGCACAGAAG 
TTAGCGCTAT CCCCACTGAG TCTCGGCAAA GAAAATCTTG CAGAGTCCTC CAAACCAACA 
GCTGGTGGCA GCAGATCACA AAAGGTCAAA GTTGCTCAGC GGAGCCCAGT AGATTCAGGC 
ACCATCCTCC GAGAACCCAC CACGAAATCC GTCCCAGTCA ATAATCTTCC TGAGAGAAGT 
CCGACTGACA GCCCCAGAGA GGGCCTGAGG GTCAAGCGAG GCCGACTTGT CCCCAGCCCC 
AAAGCTGGAC TGGAGTCCAA CGGCAGTGAG AACTGTAAGG TCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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CENPF cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

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