MYL12A cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	MYL12A
Entrez Gene ID	10627
Full Name	myosin light chain 12A
Synonyms	HEL-S-24,MLC-2B,MLCB,MRCL3,MRLC3,MYL2B
General protein information	Preferred Names myosin light chain 12A Names myosin regulatory light chain 12A epididymis secretory protein Li 24 myosin RLC myosin regulatory light chain 2, nonsarcomeric myosin regulatory light chain 3 myosin regulatory light chain MRLC3 myosin, light chain 12A, regulatory, non-sarcomeric myosin, light polypeptide, regulatory, non-sarcomeric (20kD)
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 18 Map Location: 18p11.31
Summary	This gene encodes a nonsarcomeric myosin regulatory light chain. This protein is activated by phosphorylation and regulates smooth muscle and non-muscle cell contraction. This protein may also be involved in DNA damage repair by sequestering the transcriptional regulator apoptosis-antagonizing transcription factor (AATF)/Che-1 which functions as a repressor of p53-driven apoptosis. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 8.[provided by RefSeq, Dec 2014].

mRNA and Protein(s)

mRNA	Protein	Name
NM_006471.3	NP_006462.1	myosin regulatory light chain 12A isoform 1
NM_001303048.1	NP_001289977.1	myosin regulatory light chain 12A isoform 1
XM_005258078.1	XP_005258135.1	myosin regulatory light chain 12A isoform X1
NM_001303047.1	NP_001289976.1	myosin regulatory light chain 12A isoform 1
NM_001303049.1	NP_001289978.1	myosin regulatory light chain 12A isoform 2

Pathways from BioSystems

Homologies

Bos taurus (cattle)	MYL12B	NP_001077233.1
Mus musculus (house mouse)	Myl12b	NP_075891.1
Danio rerio (zebrafish)	myl12.1	NP_999864.1
Homo sapiens (human)	MYL12A	NP_006462.1
Canis lupus familiaris (dog)	MYL12B	XP_537316.1
Rattus norvegicus (Norway rat)	Myl12b	NP_059039.2
Pan troglodytes (chimpanzee)	MYL12A	XP_001144839.1
	MYL12A	XP_001084759.2

Gene Ontology
Bibliography

Related articles in PubMed

Platelet proteome analysis reveals integrin-dependent aggregation defects in patients with myelodysplastic syndromes.
Fr?bel J, Cadeddu RP, Hartwig S, Bruns I, Wilk CM, K?ndgen A, Fischer JC, Schroeder T, Steidl UG, Germing U, Lehr S, Haas R, Czibere A
Molecular & cellular proteomics : MCP12(5)1272-80(2013 May)

AATF/Che-1 acts as a phosphorylation-dependent molecular modulator to repress p53-driven apoptosis.
H?pker K, Hagmann H, Khurshid S, Chen S, Hasskamp P, Seeger-Nukpezah T, Schilberg K, Heukamp L, Lamkemeyer T, Sos ML, Thomas RK, Lowery D, Roels F, Fischer M, Liebau MC, Resch U, Kisner T, R?ther F, Bartram MP, M?ller RU, Fabretti F, Kurschat P, Schumacher B, Gaestel M, Medema RH, Yaffe MB, Schermer B, Reinhardt HC, Benzing T
The EMBO journal31(20)3961-75(2012 Oct)

Chelerythrine perturbs lamellar actomyosin filaments by selective inhibition of myotonic dystrophy kinase-related Cdc42-binding kinase.
Tan I, Lai J, Yong J, Li SF, Leung T
FEBS letters585(9)1260-8(2011 May)

MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis.
Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W
Immunology and cell biology88(8)851-6(2010 Nov-Dec)

A physical and functional map of the human TNF-alpha/NF-kappa B signal transduction pathway.
Bouwmeester T, Bauch A, Ruffner H, Angrand PO, Bergamini G, Croughton K, Cruciat C, Eberhard D, Gagneur J, Ghidelli S, Hopf C, Huhse B, Mangano R, Michon AM, Schirle M, Schlegl J, Schwab M, Stein MA, Bauer A, Casari G, Drewes G, Gavin AC, Jackson DB, Joberty G, Neubauer G, Rick J, Kuster B, Superti-Furga G
Nature cell biology6(2)97-105(2004 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following MYL12A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MYL12A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu50970	NM_001303047.1 Latest version!	Homo sapiens myosin light chain 12A (MYL12A), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu50970	NM_001303048.1 Latest version!	Homo sapiens myosin light chain 12A (MYL12A), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu50970	NM_006471.3 Latest version!	Homo sapiens myosin light chain 12A (MYL12A), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu50970	XM_005258078.1 Latest version!	Homo sapiens myosin, light chain 12A, regulatory, non-sarcomeric (MYL12A), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu50971	NM_001303049.1 Latest version!	Homo sapiens myosin light chain 12A (MYL12A), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MYL12A ( NM_006471.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_006462.1 Homo sapiens myosin light chain 12A (MYL12A), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu50970
Clone ID Related Accession (Same CDS sequence)	NM_006471.3 , NM_001303048.1 , XM_005258078.1 , NM_001303047.1
Accession Version	NM_006471.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 516bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Homo sapiens(human)
Product	myosin regulatory light chain 12A isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC031972.1, BC032748.1 and BU615599.1. On Dec 25, 2014 this sequence version replaced NM_006471.2. Transcript Variant: This variant (1) encodes the shorter isoform (1). Variants 1, 2 and 3 encode the same isoform (1). ##Evidence-Data-START## Transcript exon combination :: BC031972.1, SRR1163657.356416.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTCGAGCA AAAGAACAAA GACCAAGACC AAGAAGCGCC CTCAGCGTGC AACATCCAAT 
GTGTTTGCTA TGTTTGACCA GTCACAGATT CAGGAGTTCA AAGAGGCCTT CAACATGATT 
GATCAGAACA GAGATGGTTT CATCGACAAG GAAGATTTGC ATGATATGCT TGCTTCATTG 
GGGAAGAATC CAACTGATGA GTATCTAGAT GCCATGATGA ATGAGGCTCC AGGCCCCATC 
AATTTCACCA TGTTCCTCAC CATGTTTGGT GAGAAGTTAA ATGGCACAGA TCCTGAAGAT 
GTCATCAGAA ATGCCTTTGC TTGCTTTGAT GAAGAAGCAA CTGGCACCAT ACAGGAAGAT 
TACTTGAGAG AGCTGCTGAC AACCATGGGG GATCGGTTTA CAGATGAGGA AGTGGATGAG 
CTGTACAGAG AAGCACCTAT TGATAAAAAG GGGAATTTCA ATTACATCGA GTTCACACGC 
ATCCTGAAAC ATGGAGCCAA AGACAAAGAT GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_006462.1
CDS	396..911
Translation	MSSKRTKTKTKKRPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASLGKNPTDEYLDAMMNEAPGPINFTMFLTMFGEKLNGTDPEDVIRNAFACFDEEATGTIQEDYLRELLTTMGDRFTDEEVDELYREAPIDKKGNFNYIEFTRILKHGAKDKDD

Target ORF information:

RefSeq Version	NM_006471.3
Organism	Homo sapiens(human)
Definition	Homo sapiens myosin light chain 12A (MYL12A), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006471.3

ORF Insert Sequence:

ATGTCGAGCA AAAGAACAAA GACCAAGACC AAGAAGCGCC CTCAGCGTGC AACATCCAAT 
GTGTTTGCTA TGTTTGACCA GTCACAGATT CAGGAGTTCA AAGAGGCCTT CAACATGATT 
GATCAGAACA GAGATGGTTT CATCGACAAG GAAGATTTGC ATGATATGCT TGCTTCATTG 
GGGAAGAATC CAACTGATGA GTATCTAGAT GCCATGATGA ATGAGGCTCC AGGCCCCATC 
AATTTCACCA TGTTCCTCAC CATGTTTGGT GAGAAGTTAA ATGGCACAGA TCCTGAAGAT 
GTCATCAGAA ATGCCTTTGC TTGCTTTGAT GAAGAAGCAA CTGGCACCAT ACAGGAAGAT 
TACTTGAGAG AGCTGCTGAC AACCATGGGG GATCGGTTTA CAGATGAGGA AGTGGATGAG 
CTGTACAGAG AAGCACCTAT TGATAAAAAG GGGAATTTCA ATTACATCGA GTTCACACGC 
ATCCTGAAAC ATGGAGCCAA AGACAAAGAT GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYL12A ( NM_001303048.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001289977.1 Homo sapiens myosin light chain 12A (MYL12A), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu50970
Clone ID Related Accession (Same CDS sequence)	NM_006471.3 , NM_001303048.1 , XM_005258078.1 , NM_001303047.1
Accession Version	NM_001303048.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 516bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Homo sapiens(human)
Product	myosin regulatory light chain 12A isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AW958488.1, CD702288.1, BC032748.1 and CA309747.1. On Dec 25, 2014 this sequence version replaced XM_005258076.1. Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1. Variants 1, 2 and 3 encode the same isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: ERR279847.9535.1, SRR1163658.131019.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCGAGCA AAAGAACAAA GACCAAGACC AAGAAGCGCC CTCAGCGTGC AACATCCAAT 
GTGTTTGCTA TGTTTGACCA GTCACAGATT CAGGAGTTCA AAGAGGCCTT CAACATGATT 
GATCAGAACA GAGATGGTTT CATCGACAAG GAAGATTTGC ATGATATGCT TGCTTCATTG 
GGGAAGAATC CAACTGATGA GTATCTAGAT GCCATGATGA ATGAGGCTCC AGGCCCCATC 
AATTTCACCA TGTTCCTCAC CATGTTTGGT GAGAAGTTAA ATGGCACAGA TCCTGAAGAT 
GTCATCAGAA ATGCCTTTGC TTGCTTTGAT GAAGAAGCAA CTGGCACCAT ACAGGAAGAT 
TACTTGAGAG AGCTGCTGAC AACCATGGGG GATCGGTTTA CAGATGAGGA AGTGGATGAG 
CTGTACAGAG AAGCACCTAT TGATAAAAAG GGGAATTTCA ATTACATCGA GTTCACACGC 
ATCCTGAAAC ATGGAGCCAA AGACAAAGAT GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001289977.1
CDS	122..637
Translation	MSSKRTKTKTKKRPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASLGKNPTDEYLDAMMNEAPGPINFTMFLTMFGEKLNGTDPEDVIRNAFACFDEEATGTIQEDYLRELLTTMGDRFTDEEVDELYREAPIDKKGNFNYIEFTRILKHGAKDKDD

Target ORF information:

RefSeq Version	NM_001303048.1
Organism	Homo sapiens(human)
Definition	Homo sapiens myosin light chain 12A (MYL12A), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001303048.1

ORF Insert Sequence:

ATGTCGAGCA AAAGAACAAA GACCAAGACC AAGAAGCGCC CTCAGCGTGC AACATCCAAT 
GTGTTTGCTA TGTTTGACCA GTCACAGATT CAGGAGTTCA AAGAGGCCTT CAACATGATT 
GATCAGAACA GAGATGGTTT CATCGACAAG GAAGATTTGC ATGATATGCT TGCTTCATTG 
GGGAAGAATC CAACTGATGA GTATCTAGAT GCCATGATGA ATGAGGCTCC AGGCCCCATC 
AATTTCACCA TGTTCCTCAC CATGTTTGGT GAGAAGTTAA ATGGCACAGA TCCTGAAGAT 
GTCATCAGAA ATGCCTTTGC TTGCTTTGAT GAAGAAGCAA CTGGCACCAT ACAGGAAGAT 
TACTTGAGAG AGCTGCTGAC AACCATGGGG GATCGGTTTA CAGATGAGGA AGTGGATGAG 
CTGTACAGAG AAGCACCTAT TGATAAAAAG GGGAATTTCA ATTACATCGA GTTCACACGC 
ATCCTGAAAC ATGGAGCCAA AGACAAAGAT GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYL12A ( NM_001303047.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001289976.1 Homo sapiens myosin light chain 12A (MYL12A), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu50970
Clone ID Related Accession (Same CDS sequence)	NM_006471.3 , NM_001303048.1 , XM_005258078.1 , NM_001303047.1
Accession Version	NM_001303047.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 516bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Homo sapiens(human)
Product	myosin regulatory light chain 12A isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AP005329.3, BM561281.1, BC032748.1 and CA309747.1. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1, 2 and 3 encode the same isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1163655.196671.1, SRR1163657.573260.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCGAGCA AAAGAACAAA GACCAAGACC AAGAAGCGCC CTCAGCGTGC AACATCCAAT 
GTGTTTGCTA TGTTTGACCA GTCACAGATT CAGGAGTTCA AAGAGGCCTT CAACATGATT 
GATCAGAACA GAGATGGTTT CATCGACAAG GAAGATTTGC ATGATATGCT TGCTTCATTG 
GGGAAGAATC CAACTGATGA GTATCTAGAT GCCATGATGA ATGAGGCTCC AGGCCCCATC 
AATTTCACCA TGTTCCTCAC CATGTTTGGT GAGAAGTTAA ATGGCACAGA TCCTGAAGAT 
GTCATCAGAA ATGCCTTTGC TTGCTTTGAT GAAGAAGCAA CTGGCACCAT ACAGGAAGAT 
TACTTGAGAG AGCTGCTGAC AACCATGGGG GATCGGTTTA CAGATGAGGA AGTGGATGAG 
CTGTACAGAG AAGCACCTAT TGATAAAAAG GGGAATTTCA ATTACATCGA GTTCACACGC 
ATCCTGAAAC ATGGAGCCAA AGACAAAGAT GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001289976.1
CDS	479..994
Translation	MSSKRTKTKTKKRPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASLGKNPTDEYLDAMMNEAPGPINFTMFLTMFGEKLNGTDPEDVIRNAFACFDEEATGTIQEDYLRELLTTMGDRFTDEEVDELYREAPIDKKGNFNYIEFTRILKHGAKDKDD

Target ORF information:

RefSeq Version	NM_001303047.1
Organism	Homo sapiens(human)
Definition	Homo sapiens myosin light chain 12A (MYL12A), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001303047.1

ORF Insert Sequence:

ATGTCGAGCA AAAGAACAAA GACCAAGACC AAGAAGCGCC CTCAGCGTGC AACATCCAAT 
GTGTTTGCTA TGTTTGACCA GTCACAGATT CAGGAGTTCA AAGAGGCCTT CAACATGATT 
GATCAGAACA GAGATGGTTT CATCGACAAG GAAGATTTGC ATGATATGCT TGCTTCATTG 
GGGAAGAATC CAACTGATGA GTATCTAGAT GCCATGATGA ATGAGGCTCC AGGCCCCATC 
AATTTCACCA TGTTCCTCAC CATGTTTGGT GAGAAGTTAA ATGGCACAGA TCCTGAAGAT 
GTCATCAGAA ATGCCTTTGC TTGCTTTGAT GAAGAAGCAA CTGGCACCAT ACAGGAAGAT 
TACTTGAGAG AGCTGCTGAC AACCATGGGG GATCGGTTTA CAGATGAGGA AGTGGATGAG 
CTGTACAGAG AAGCACCTAT TGATAAAAAG GGGAATTTCA ATTACATCGA GTTCACACGC 
ATCCTGAAAC ATGGAGCCAA AGACAAAGAT GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYL12A ( XM_005258078.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_005258135.1 Homo sapiens myosin, light chain 12A, regulatory, non-sarcomeric (MYL12A), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu50970
Clone ID Related Accession (Same CDS sequence)	NM_006471.3 , NM_001303048.1 , XM_005258078.1 , NM_001303047.1
Accession Version	XM_005258078.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 516bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	myosin regulatory light chain 12A isoform X1
Comment

ATGTCGAGCA AAAGAACAAA GACCAAGACC AAGAAGCGCC CTCAGCGTGC AACATCCAAT 
GTGTTTGCTA TGTTTGACCA GTCACAGATT CAGGAGTTCA AAGAGGCCTT CAACATGATT 
GATCAGAACA GAGATGGTTT CATCGACAAG GAAGATTTGC ATGATATGCT TGCTTCATTG 
GGGAAGAATC CAACTGATGA GTATCTAGAT GCCATGATGA ATGAGGCTCC AGGCCCCATC 
AATTTCACCA TGTTCCTCAC CATGTTTGGT GAGAAGTTAA ATGGCACAGA TCCTGAAGAT 
GTCATCAGAA ATGCCTTTGC TTGCTTTGAT GAAGAAGCAA CTGGCACCAT ACAGGAAGAT 
TACTTGAGAG AGCTGCTGAC AACCATGGGG GATCGGTTTA CAGATGAGGA AGTGGATGAG 
CTGTACAGAG AAGCACCTAT TGATAAAAAG GGGAATTTCA ATTACATCGA GTTCACACGC 
ATCCTGAAAC ATGGAGCCAA AGACAAAGAT GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005258135.1
Translation	MSSKRTKTKTKKRPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASLGKNPTDEYLDAMMNEAPGPINFTMFLTMFGEKLNGTDPEDVIRNAFACFDEEATGTIQEDYLRELLTTMGDRFTDEEVDELYREAPIDKKGNFNYIEFTRILKHGAKDKDD

Target ORF information:

RefSeq Version	XM_005258078.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens myosin, light chain 12A, regulatory, non-sarcomeric (MYL12A), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005258078.1

ORF Insert Sequence:

ATGTCGAGCA AAAGAACAAA GACCAAGACC AAGAAGCGCC CTCAGCGTGC AACATCCAAT 
GTGTTTGCTA TGTTTGACCA GTCACAGATT CAGGAGTTCA AAGAGGCCTT CAACATGATT 
GATCAGAACA GAGATGGTTT CATCGACAAG GAAGATTTGC ATGATATGCT TGCTTCATTG 
GGGAAGAATC CAACTGATGA GTATCTAGAT GCCATGATGA ATGAGGCTCC AGGCCCCATC 
AATTTCACCA TGTTCCTCAC CATGTTTGGT GAGAAGTTAA ATGGCACAGA TCCTGAAGAT 
GTCATCAGAA ATGCCTTTGC TTGCTTTGAT GAAGAAGCAA CTGGCACCAT ACAGGAAGAT 
TACTTGAGAG AGCTGCTGAC AACCATGGGG GATCGGTTTA CAGATGAGGA AGTGGATGAG 
CTGTACAGAG AAGCACCTAT TGATAAAAAG GGGAATTTCA ATTACATCGA GTTCACACGC 
ATCCTGAAAC ATGGAGCCAA AGACAAAGAT GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYL12A ( NM_001303049.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001289978.1 Homo sapiens myosin light chain 12A (MYL12A), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu50971
Clone ID Related Accession (Same CDS sequence)	NM_001303049.1
Accession Version	NM_001303049.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 534bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-19
Organism	Homo sapiens(human)
Product	myosin regulatory light chain 12A isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AP005329.3, BC016372.1, AB046614.1 and BU615599.1. On Dec 25, 2014 this sequence version replaced XM_005258077.2. Transcript Variant: This variant (4) uses an alternate exon in the 5' UTR and 5' coding region compared to variant 1. These differences result in the use of an alternate start codon compared to variant 1. The encoded isoform (2) has a longer N-terminus compare to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: ERR279838.10614.1, ERR279846.130.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA2142853 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACTTAA CCACCACCAT GTCGAGCAAA AGAACAAAGA CCAAGACCAA GAAGCGCCCT 
CAGCGTGCAA CATCCAATGT GTTTGCTATG TTTGACCAGT CACAGATTCA GGAGTTCAAA 
GAGGCCTTCA ACATGATTGA TCAGAACAGA GATGGTTTCA TCGACAAGGA AGATTTGCAT 
GATATGCTTG CTTCATTGGG GAAGAATCCA ACTGATGAGT ATCTAGATGC CATGATGAAT 
GAGGCTCCAG GCCCCATCAA TTTCACCATG TTCCTCACCA TGTTTGGTGA GAAGTTAAAT 
GGCACAGATC CTGAAGATGT CATCAGAAAT GCCTTTGCTT GCTTTGATGA AGAAGCAACT 
GGCACCATAC AGGAAGATTA CTTGAGAGAG CTGCTGACAA CCATGGGGGA TCGGTTTACA 
GATGAGGAAG TGGATGAGCT GTACAGAGAA GCACCTATTG ATAAAAAGGG GAATTTCAAT 
TACATCGAGT TCACACGCAT CCTGAAACAT GGAGCCAAAG ACAAAGATGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001289978.1
CDS	111..644
Translation	MDLTTTMSSKRTKTKTKKRPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASLGKNPTDEYLDAMMNEAPGPINFTMFLTMFGEKLNGTDPEDVIRNAFACFDEEATGTIQEDYLRELLTTMGDRFTDEEVDELYREAPIDKKGNFNYIEFTRILKHGAKDKDD

Target ORF information:

RefSeq Version	NM_001303049.1
Organism	Homo sapiens(human)
Definition	Homo sapiens myosin light chain 12A (MYL12A), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001303049.1

ORF Insert Sequence:

ATGGACTTAA CCACCACCAT GTCGAGCAAA AGAACAAAGA CCAAGACCAA GAAGCGCCCT 
CAGCGTGCAA CATCCAATGT GTTTGCTATG TTTGACCAGT CACAGATTCA GGAGTTCAAA 
GAGGCCTTCA ACATGATTGA TCAGAACAGA GATGGTTTCA TCGACAAGGA AGATTTGCAT 
GATATGCTTG CTTCATTGGG GAAGAATCCA ACTGATGAGT ATCTAGATGC CATGATGAAT 
GAGGCTCCAG GCCCCATCAA TTTCACCATG TTCCTCACCA TGTTTGGTGA GAAGTTAAAT 
GGCACAGATC CTGAAGATGT CATCAGAAAT GCCTTTGCTT GCTTTGATGA AGAAGCAACT 
GGCACCATAC AGGAAGATTA CTTGAGAGAG CTGCTGACAA CCATGGGGGA TCGGTTTACA 
GATGAGGAAG TGGATGAGCT GTACAGAGAA GCACCTATTG ATAAAAAGGG GAATTTCAAT 
TACATCGAGT TCACACGCAT CCTGAAACAT GGAGCCAAAG ACAAAGATGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Platelet proteome analysis reveals integrin-dependent aggregation defects in patients with myelodysplastic syndromes.
Molecular & cellular proteomics : MCP12(5)1272-80(2013 May)
Fr?bel J,Cadeddu RP,Hartwig S,Bruns I,Wilk CM,K?ndgen A,Fischer JC,Schroeder T,Steidl UG,Germing U,Lehr S,Haas R,Czibere A

AATF/Che-1 acts as a phosphorylation-dependent molecular modulator to repress p53-driven apoptosis.
The EMBO journal31(20)3961-75(2012 Oct)
H?pker K,Hagmann H,Khurshid S,Chen S,Hasskamp P,Seeger-Nukpezah T,Schilberg K,Heukamp L,Lamkemeyer T,Sos ML,Thomas RK,Lowery D,Roels F,Fischer M,Liebau MC,Resch U,Kisner T,R?ther F,Bartram MP,M?ller RU,Fabretti F,Kurschat P,Schumacher B,Gaestel M,Medema RH,Yaffe MB,Schermer B,Reinhardt HC,Benzing T

Chelerythrine perturbs lamellar actomyosin filaments by selective inhibition of myotonic dystrophy kinase-related Cdc42-binding kinase.
FEBS letters585(9)1260-8(2011 May)
Tan I,Lai J,Yong J,Li SF,Leung T

MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis.
Immunology and cell biology88(8)851-6(2010 Nov-Dec)
Buschow SI,van Balkom BW,Aalberts M,Heck AJ,Wauben M,Stoorvogel W

A physical and functional map of the human TNF-alpha/NF-kappa B signal transduction pathway.
Nature cell biology6(2)97-105(2004 Feb)
Bouwmeester T,Bauch A,Ruffner H,Angrand PO,Bergamini G,Croughton K,Cruciat C,Eberhard D,Gagneur J,Ghidelli S,Hopf C,Huhse B,Mangano R,Michon AM,Schirle M,Schlegl J,Schwab M,Stein MA,Bauer A,Casari G,Drewes G,Gavin AC,Jackson DB,Joberty G,Neubauer G,Rick J,Kuster B,Superti-Furga G

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MYL12A cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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