SPAG5 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	SPAG5
Entrez Gene ID	10615
Full Name	sperm associated antigen 5
Synonyms	DEEPEST,MAP126,hMAP126
General protein information	Preferred Names sperm associated antigen 5 Names sperm-associated antigen 5 astrin mitotic spindle associated protein p126 mitotic spindle coiled-coil related protein
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 17 Map Location: 17q11.2
Summary	This gene encodes a protein associated with the mitotic spindle apparatus. The encoded protein may be involved in the functional and dynamic regulation of mitotic spindles. [provided by RefSeq, Jul 2008].
Disorder MIM:	615562

mRNA and Protein(s)

mRNA	Protein	Name
NM_006461.3	NP_006452.3	sperm-associated antigen 5
NM_006461.4	NP_006452.3	sperm-associated antigen 5

Pathways from BioSystems

Homologies

	SPAG5	XP_001108753.2
Canis lupus familiaris (dog)	SPAG5	XP_537743.4
Homo sapiens (human)	SPAG5	NP_006452.3
Bos taurus (cattle)	SPAG5	NP_001192478.1
Mus musculus (house mouse)	Spag5	NP_059103.1
Rattus norvegicus (Norway rat)	Spag5	NP_001037689.1
Gallus gallus (chicken)	SPAG5	XP_004946625.1

Gene Ontology

The following SPAG5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPAG5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu13103	NM_006461.4 Latest version!	Homo sapiens sperm associated antigen 5 (SPAG5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu13103	NM_006461.3	Homo sapiens sperm associated antigen 5 (SPAG5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SPAG5 ( NM_006461.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_006452.3 Homo sapiens sperm associated antigen 5 (SPAG5), mRNA.

Price & Availability↑

CloneID	OHu13103
Clone ID Related Accession (Same CDS sequence)	NM_006461.3 , NM_006461.4
Accession Version	NM_006461.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3582bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-11-19
Organism	Homo sapiens(human)
Product	sperm-associated antigen 5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BI459711.1, BC000322.2 and BQ773438.1. On Aug 19, 2005 this sequence version replaced NM_006461.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF063308.1, AF399910.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTGGCGAG TGAAAAAACT GAGCCTCAGC CTGTCGCCTT CGCCCCAGAC GGGAAAACCA 
TCTATGAGAA CTCCTCTCCG TGAACTTACC CTGCAGCCCG GTGCCCTCAC CAACTCTGGA 
AAAAGATCCC CCGCTTGCTC CTCGCTGACC CCATCACTGT GCAAGCTGGG GCTGCAGGAA 
GGCAGCAACA ACTCATCTCC AGTGGATTTT GTAAATAACA AGAGGACAGA CTTATCTTCA 
GAACATTTCA GTCATTCCTC AAAGTGGCTA GAAACTTGTC AGCATGAATC AGATGAGCAG 
CCTCTAGATC CAATTCCCCA AATTAGCTCT ACTCCTAAAA CGTCTGAGGA AGCAGTAGAC 
CCACTGGGCA ATTATATGGT TAAAACCATC GTCCTTGTAC CATCTCCACT GGGGCAGCAA 
CAAGACATGA TATTTGAGGC CCGTTTAGAT ACCATGGCAG AGACAAACAG CATATCTTTA 
AATGGACCTT TGAGAACAGA CGATCTGGTG AGAGAGGAGG TGGCACCCTG CATGGGAGAC 
AGGTTTTCAG AAGTTGCTGC TGTATCTGAG AAACCTATCT TTCAGGAATC TCCGTCCCAT 
CTCTTAGAGG AGTCTCCACC AAATCCCTGT TCTGAACAAC TACATTGCTC CAAGGAAAGC 
CTGAGCAGTA GAACTGAGGC TGTGCGTGAG GACTTAGTAC CTTCTGAAAG TAACGCCTTC 
TTGCCTTCCT CTGTTCTCTG GCTTTCCCCT TCAACTGCCT TGGCAGCAGA TTTCCGTGTC 
AATCATGTGG ACCCAGAGGA GGAAATTGTA GAGCATGGAG CTATGGAGGA AAGAGAAATG 
AGGTTTCCCA CACATCCTAA GGAGTCTGAA ACAGAAGATC AAGCACTTGT CTCAAGTGTG 
GAAGATATTC TGTCCACATG CCTGACACCA AATCTAGTAG AAATGGAATC CCAAGAAGCT 
CCAGGCCCAG CAGTAGAAGA TGTTGGTAGG ATTCTTGGCT CTGATACAGA GTCTTGGATG 
TCCCCACTGG CCTGGCTGGA AAAAGGTGTA AATACCTCCG TCATGCTGGA AAATCTCCGC 
CAAAGCTTAT CCCTTCCCTC GATGCTTCGG GATGCTGCAA TTGGCACTAC CCCTTTCTCT 
ACTTGCTCGG TGGGGACTTG GTTTACTCCT TCAGCACCAC AGGAAAAGAG TACAAACACA 
TCCCAGACAG GCCTGGTTGG CACCAAGCAC AGTACTTCTG AGACAGAGCA GCTCCTGTGT 
GGCCGGCCTC CAGATCTGAC TGCCTTGTCT CGACATGACT TGGAAGATAA CCTGCTGAGC 
TCTCTTGTCA TTCTGGAGGT TCTCTCCCGC CAGCTTCGGG ACTGGAAGAG CCAGCTGGCT 
GTCCCTCACC CAGAAACCCA GGACAGTAGC ACACAGACTG ACACATCTCA CAGTGGGATA 
ACTAATAAAC TTCAGCATCT TAAGGAGAGC CATGAGATGG GACAGGCCCT ACAGCAGGCC 
AGAAATGTCA TGCAATCATG GGTGCTTATC TCTAAAGAGC TGATATCCTT GCTTCACCTA 
TCCCTGTTGC ATTTAGAAGA AGATAAGACT ACTGTGAGTC AGGAGTCTCG GCGTGCAGAA 
ACATTGGTCT GTTGCTGTTT TGATTTGCTG AAGAAATTGA GGGCAAAGCT CCAGAGCCTC 
AAAGCAGAAA GGGAGGAGGC AAGGCACAGA GAGGAAATGG CTCTCAGAGG CAAGGATGCG 
GCAGAGATAG TGTTGGAGGC TTTCTGTGCA CACGCCAGCC AGCGCATCAG CCAGCTGGAA 
CAGGACCTAG CATCCATGCG GGAATTCAGA GGCCTTCTGA AGGATGCCCA GACCCAACTG 
GTAGGGCTTC ATGCCAAGCA AGAAGAGCTG GTTCAGCAGA CAGTGAGTCT TACTTCTACC 
TTGCAACAAG ACTGGAGGTC CATGCAACTG GATTATACAA CATGGACAGC TTTGCTGAGT 
CGGTCCCGAC AACTCACAGA GAAACTCACA GTCAAGAGCC AGCAAGCCCT GCAGGAACGT 
GATGTGGCAA TTGAGGAAAA GCAGGAGGTT TCTAGGGTGC TGGAACAAGT CTCTGCCCAG 
TTAGAGGAGT GCAAAGGCCA AACAGAACAA CTGGAGTTGG AAAACAGTCG TCTAGCAACA 
GATCTCCGGG CTCAGTTGCA GATTCTGGCC AACATGGACA GCCAGCTAAA AGAGCTACAG 
AGTCAGCATA CCCATTGTGC CCAGGACCTG GCTATGAAGG ATGAGTTACT CTGCCAGCTT 
ACCCAGAGCA ATGAGGAGCA GGCTGCTCAA TGGCAAAAGG AAGAGATGGC ACTAAAACAC 
ATGCAGGCAG AACTGCAGCA GCAACAAGCT GTCCTGGCCA AAGAGGTGCG GGACCTGAAA 
GAGACCTTGG AGTTTGCAGA CCAGGAGAAT CAGGTTGCTC ACCTGGAGCT GGGTCAGGTT 
GAGTGTCAAT TGAAAACCAC ACTGGAAGTG CTCCGGGAGC GCAGCTTGCA GTGTGAGAAC 
CTCAAGGACA CTGTAGAGAA CCTAACGGCT AAACTGGCCA GCACCATAGC AGATAACCAG 
GAGCAAGATC TGGAGAAAAC ACGGCAGTAC TCTCAAAAGC TAGGGCTGCT GACTGAGCAA 
CTACAGAGCC TGACTCTCTT TCTACAGACA AAACTAAAGG AGAAGACTGA ACAAGAGACC 
CTTCTGCTGA GTACAGCCTG TCCTCCCACC CAGGAACACC CTCTGCCTAA TGACAGGACC 
TTCCTGGGAA GCATCTTGAC AGCAGTGGCA GATGAAGAGC CAGAATCAAC TCCTGTGCCC 
TTGCTTGGAA GTGACAAGAG TGCTTTCACC CGAGTAGCAT CAATGGTTTC CCTTCAGCCC 
GCAGAGACCC CAGGCATGGA GGAGAGCCTG GCAGAAATGA GTATTATGAC TACTGAGCTT 
CAGAGTCTTT GTTCCCTGCT ACAAGAGTCT AAAGAAGAAG CCATCAGGAC TCTGCAGCGA 
AAAATTTGTG AGCTGCAAGC TAGGCTGCAG GCCCAGGAAG AACAGCATCA GGAAGTCCAG 
AAGGCAAAAG AAGCAGACAT AGAGAAGCTG AACCAGGCCT TGTGCTTGCG CTACAAGAAT 
GAAAAGGAGC TCCAGGAAGT GATACAGCAG CAGAATGAGA AGATCCTAGA ACAGATAGAC 
AAGAGTGGCG AGCTCATAAG CCTTAGAGAG GAGGTGACCC ACCTTACCCG CTCACTTCGG 
CGTGCGGAGA CAGAGACCAA AGTGCTCCAG GAGGCCCTGG CAGGCCAGCT GGACTCCAAC 
TGCCAGCCTA TGGCCACCAA TTGGATCCAG GAGAAAGTGT GGCTCTCTCA GGAGGTGGAC 
AAACTGAGAG TGATGTTCCT GGAGATGAAA AATGAGAAGG AAAAACTCAT GATCAAGTTC 
CAGAGCCATA GAAATATCCT AGAGGAGAAC CTTCGGCGCT CTGACAAGGA GTTAGAAAAA 
CTAGATGACA TTGTTCAGCA TATTTATAAG ACCCTGCTCT CTATTCCAGA GGTGGTGAGG 
GGATGCAAAG AACTACAGGG ATTGCTGGAA TTTCTGAGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_006452.3
CDS	93..3674
Translation	MWRVKKLSLSLSPSPQTGKPSMRTPLRELTLQPGALTNSGKRSPACSSLTPSLCKLGLQEGSNNSSPVDFVNNKRTDLSSEHFSHSSKWLETCQHESDEQPLDPIPQISSTPKTSEEAVDPLGNYMVKTIVLVPSPLGQQQDMIFEARLDTMAETNSISLNGPLRTDDLVREEVAPCMGDRFSEVAAVSEKPIFQESPSHLLEESPPNPCSEQLHCSKESLSSRTEAVREDLVPSESNAFLPSSVLWLSPSTALAADFRVNHVDPEEEIVEHGAMEEREMRFPTHPKESETEDQALVSSVEDILSTCLTPNLVEMESQEAPGPAVEDVGRILGSDTESWMSPLAWLEKGVNTSVMLENLRQSLSLPSMLRDAAIGTTPFSTCSVGTWFTPSAPQEKSTNTSQTGLVGTKHSTSETEQLLCGRPPDLTALSRHDLEDNLLSSLVILEVLSRQLRDWKSQLAVPHPETQDSSTQTDTSHSGITNKLQHLKESHEMGQALQQARNVMQSWVLISKELISLLHLSLLHLEEDKTTVSQESRRAETLVCCCFDLLKKLRAKLQSLKAEREEARHREEMALRGKDAAEIVLEAFCAHASQRISQLEQDLASMREFRGLLKDAQTQLVGLHAKQEELVQQTVSLTSTLQQDWRSMQLDYTTWTALLSRSRQLTEKLTVKSQQALQERDVAIEEKQEVSRVLEQVSAQLEECKGQTEQLELENSRLATDLRAQLQILANMDSQLKELQSQHTHCAQDLAMKDELLCQLTQSNEEQAAQWQKEEMALKHMQAELQQQQAVLAKEVRDLKETLEFADQENQVAHLELGQVECQLKTTLEVLRERSLQCENLKDTVENLTAKLASTIADNQEQDLEKTRQYSQKLGLLTEQLQSLTLFLQTKLKEKTEQETLLLSTACPPTQEHPLPNDRTFLGSILTAVADEEPESTPVPLLGSDKSAFTRVASMVSLQPAETPGMEESLAEMSIMTTELQSLCSLLQESKEEAIRTLQRKICELQARLQAQEEQHQEVQKAKEADIEKLNQALCLRYKNEKELQEVIQQQNEKILEQIDKSGELISLREEVTHLTRSLRRAETETKVLQEALAGQLDSNCQPMATNWIQEKVWLSQEVDKLRVMFLEMKNEKEKLMIKFQSHRNILEENLRRSDKELEKLDDIVQHIYKTLLSIPEVVRGCKELQGLLEFLS

Target ORF information:

RefSeq Version	NM_006461.3
Organism	Homo sapiens(human)
Definition	Homo sapiens sperm associated antigen 5 (SPAG5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006461.3

ORF Insert Sequence:

ATGTGGCGAG TGAAAAAACT GAGCCTCAGC CTGTCGCCTT CGCCCCAGAC GGGAAAACCA 
TCTATGAGAA CTCCTCTCCG TGAACTTACC CTGCAGCCCG GTGCCCTCAC CAACTCTGGA 
AAAAGATCCC CCGCTTGCTC CTCGCTGACC CCATCACTGT GCAAGCTGGG GCTGCAGGAA 
GGCAGCAACA ACTCATCTCC AGTGGATTTT GTAAATAACA AGAGGACAGA CTTATCTTCA 
GAACATTTCA GTCATTCCTC AAAGTGGCTA GAAACTTGTC AGCATGAATC AGATGAGCAG 
CCTCTAGATC CAATTCCCCA AATTAGCTCT ACTCCTAAAA CGTCTGAGGA AGCAGTAGAC 
CCACTGGGCA ATTATATGGT TAAAACCATC GTCCTTGTAC CATCTCCACT GGGGCAGCAA 
CAAGACATGA TATTTGAGGC CCGTTTAGAT ACCATGGCAG AGACAAACAG CATATCTTTA 
AATGGACCTT TGAGAACAGA CGATCTGGTG AGAGAGGAGG TGGCACCCTG CATGGGAGAC 
AGGTTTTCAG AAGTTGCTGC TGTATCTGAG AAACCTATCT TTCAGGAATC TCCGTCCCAT 
CTCTTAGAGG AGTCTCCACC AAATCCCTGT TCTGAACAAC TACATTGCTC CAAGGAAAGC 
CTGAGCAGTA GAACTGAGGC TGTGCGTGAG GACTTAGTAC CTTCTGAAAG TAACGCCTTC 
TTGCCTTCCT CTGTTCTCTG GCTTTCCCCT TCAACTGCCT TGGCAGCAGA TTTCCGTGTC 
AATCATGTGG ACCCAGAGGA GGAAATTGTA GAGCATGGAG CTATGGAGGA AAGAGAAATG 
AGGTTTCCCA CACATCCTAA GGAGTCTGAA ACAGAAGATC AAGCACTTGT CTCAAGTGTG 
GAAGATATTC TGTCCACATG CCTGACACCA AATCTAGTAG AAATGGAATC CCAAGAAGCT 
CCAGGCCCAG CAGTAGAAGA TGTTGGTAGG ATTCTTGGCT CTGATACAGA GTCTTGGATG 
TCCCCACTGG CCTGGCTGGA AAAAGGTGTA AATACCTCCG TCATGCTGGA AAATCTCCGC 
CAAAGCTTAT CCCTTCCCTC GATGCTTCGG GATGCTGCAA TTGGCACTAC CCCTTTCTCT 
ACTTGCTCGG TGGGGACTTG GTTTACTCCT TCAGCACCAC AGGAAAAGAG TACAAACACA 
TCCCAGACAG GCCTGGTTGG CACCAAGCAC AGTACTTCTG AGACAGAGCA GCTCCTGTGT 
GGCCGGCCTC CAGATCTGAC TGCCTTGTCT CGACATGACT TGGAAGATAA CCTGCTGAGC 
TCTCTTGTCA TTCTGGAGGT TCTCTCCCGC CAGCTTCGGG ACTGGAAGAG CCAGCTGGCT 
GTCCCTCACC CAGAAACCCA GGACAGTAGC ACACAGACTG ACACATCTCA CAGTGGGATA 
ACTAATAAAC TTCAGCATCT TAAGGAGAGC CATGAGATGG GACAGGCCCT ACAGCAGGCC 
AGAAATGTCA TGCAATCATG GGTGCTTATC TCTAAAGAGC TGATATCCTT GCTTCACCTA 
TCCCTGTTGC ATTTAGAAGA AGATAAGACT ACTGTGAGTC AGGAGTCTCG GCGTGCAGAA 
ACATTGGTCT GTTGCTGTTT TGATTTGCTG AAGAAATTGA GGGCAAAGCT CCAGAGCCTC 
AAAGCAGAAA GGGAGGAGGC AAGGCACAGA GAGGAAATGG CTCTCAGAGG CAAGGATGCG 
GCAGAGATAG TGTTGGAGGC TTTCTGTGCA CACGCCAGCC AGCGCATCAG CCAGCTGGAA 
CAGGACCTAG CATCCATGCG GGAATTCAGA GGCCTTCTGA AGGATGCCCA GACCCAACTG 
GTAGGGCTTC ATGCCAAGCA AGAAGAGCTG GTTCAGCAGA CAGTGAGTCT TACTTCTACC 
TTGCAACAAG ACTGGAGGTC CATGCAACTG GATTATACAA CATGGACAGC TTTGCTGAGT 
CGGTCCCGAC AACTCACAGA GAAACTCACA GTCAAGAGCC AGCAAGCCCT GCAGGAACGT 
GATGTGGCAA TTGAGGAAAA GCAGGAGGTT TCTAGGGTGC TGGAACAAGT CTCTGCCCAG 
TTAGAGGAGT GCAAAGGCCA AACAGAACAA CTGGAGTTGG AAAACAGTCG TCTAGCAACA 
GATCTCCGGG CTCAGTTGCA GATTCTGGCC AACATGGACA GCCAGCTAAA AGAGCTACAG 
AGTCAGCATA CCCATTGTGC CCAGGACCTG GCTATGAAGG ATGAGTTACT CTGCCAGCTT 
ACCCAGAGCA ATGAGGAGCA GGCTGCTCAA TGGCAAAAGG AAGAGATGGC ACTAAAACAC 
ATGCAGGCAG AACTGCAGCA GCAACAAGCT GTCCTGGCCA AAGAGGTGCG GGACCTGAAA 
GAGACCTTGG AGTTTGCAGA CCAGGAGAAT CAGGTTGCTC ACCTGGAGCT GGGTCAGGTT 
GAGTGTCAAT TGAAAACCAC ACTGGAAGTG CTCCGGGAGC GCAGCTTGCA GTGTGAGAAC 
CTCAAGGACA CTGTAGAGAA CCTAACGGCT AAACTGGCCA GCACCATAGC AGATAACCAG 
GAGCAAGATC TGGAGAAAAC ACGGCAGTAC TCTCAAAAGC TAGGGCTGCT GACTGAGCAA 
CTACAGAGCC TGACTCTCTT TCTACAGACA AAACTAAAGG AGAAGACTGA ACAAGAGACC 
CTTCTGCTGA GTACAGCCTG TCCTCCCACC CAGGAACACC CTCTGCCTAA TGACAGGACC 
TTCCTGGGAA GCATCTTGAC AGCAGTGGCA GATGAAGAGC CAGAATCAAC TCCTGTGCCC 
TTGCTTGGAA GTGACAAGAG TGCTTTCACC CGAGTAGCAT CAATGGTTTC CCTTCAGCCC 
GCAGAGACCC CAGGCATGGA GGAGAGCCTG GCAGAAATGA GTATTATGAC TACTGAGCTT 
CAGAGTCTTT GTTCCCTGCT ACAAGAGTCT AAAGAAGAAG CCATCAGGAC TCTGCAGCGA 
AAAATTTGTG AGCTGCAAGC TAGGCTGCAG GCCCAGGAAG AACAGCATCA GGAAGTCCAG 
AAGGCAAAAG AAGCAGACAT AGAGAAGCTG AACCAGGCCT TGTGCTTGCG CTACAAGAAT 
GAAAAGGAGC TCCAGGAAGT GATACAGCAG CAGAATGAGA AGATCCTAGA ACAGATAGAC 
AAGAGTGGCG AGCTCATAAG CCTTAGAGAG GAGGTGACCC ACCTTACCCG CTCACTTCGG 
CGTGCGGAGA CAGAGACCAA AGTGCTCCAG GAGGCCCTGG CAGGCCAGCT GGACTCCAAC 
TGCCAGCCTA TGGCCACCAA TTGGATCCAG GAGAAAGTGT GGCTCTCTCA GGAGGTGGAC 
AAACTGAGAG TGATGTTCCT GGAGATGAAA AATGAGAAGG AAAAACTCAT GATCAAGTTC 
CAGAGCCATA GAAATATCCT AGAGGAGAAC CTTCGGCGCT CTGACAAGGA GTTAGAAAAA 
CTAGATGACA TTGTTCAGCA TATTTATAAG ACCCTGCTCT CTATTCCAGA GGTGGTGAGG 
GGATGCAAAG AACTACAGGG ATTGCTGGAA TTTCTGAGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPAG5 ( NM_006461.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_006452.3 Homo sapiens sperm associated antigen 5 (SPAG5), mRNA.

Price & Availability↑

CloneID	OHu13103
Clone ID Related Accession (Same CDS sequence)	NM_006461.3 , NM_006461.4
Accession Version	NM_006461.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3582bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-07
Organism	Homo sapiens(human)
Product	sperm-associated antigen 5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BI459711.1, BC000322.2 and BQ773438.1. On Nov 23, 2018 this sequence version replaced NM_006461.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF063308.1, SRR1660807.158831.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000321765.10/ ENSP00000323300.5 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTGGCGAG TGAAAAAACT GAGCCTCAGC CTGTCGCCTT CGCCCCAGAC GGGAAAACCA 
TCTATGAGAA CTCCTCTCCG TGAACTTACC CTGCAGCCCG GTGCCCTCAC CAACTCTGGA 
AAAAGATCCC CCGCTTGCTC CTCGCTGACC CCATCACTGT GCAAGCTGGG GCTGCAGGAA 
GGCAGCAACA ACTCATCTCC AGTGGATTTT GTAAATAACA AGAGGACAGA CTTATCTTCA 
GAACATTTCA GTCATTCCTC AAAGTGGCTA GAAACTTGTC AGCATGAATC AGATGAGCAG 
CCTCTAGATC CAATTCCCCA AATTAGCTCT ACTCCTAAAA CGTCTGAGGA AGCAGTAGAC 
CCACTGGGCA ATTATATGGT TAAAACCATC GTCCTTGTAC CATCTCCACT GGGGCAGCAA 
CAAGACATGA TATTTGAGGC CCGTTTAGAT ACCATGGCAG AGACAAACAG CATATCTTTA 
AATGGACCTT TGAGAACAGA CGATCTGGTG AGAGAGGAGG TGGCACCCTG CATGGGAGAC 
AGGTTTTCAG AAGTTGCTGC TGTATCTGAG AAACCTATCT TTCAGGAATC TCCGTCCCAT 
CTCTTAGAGG AGTCTCCACC AAATCCCTGT TCTGAACAAC TACATTGCTC CAAGGAAAGC 
CTGAGCAGTA GAACTGAGGC TGTGCGTGAG GACTTAGTAC CTTCTGAAAG TAACGCCTTC 
TTGCCTTCCT CTGTTCTCTG GCTTTCCCCT TCAACTGCCT TGGCAGCAGA TTTCCGTGTC 
AATCATGTGG ACCCAGAGGA GGAAATTGTA GAGCATGGAG CTATGGAGGA AAGAGAAATG 
AGGTTTCCCA CACATCCTAA GGAGTCTGAA ACAGAAGATC AAGCACTTGT CTCAAGTGTG 
GAAGATATTC TGTCCACATG CCTGACACCA AATCTAGTAG AAATGGAATC CCAAGAAGCT 
CCAGGCCCAG CAGTAGAAGA TGTTGGTAGG ATTCTTGGCT CTGATACAGA GTCTTGGATG 
TCCCCACTGG CCTGGCTGGA AAAAGGTGTA AATACCTCCG TCATGCTGGA AAATCTCCGC 
CAAAGCTTAT CCCTTCCCTC GATGCTTCGG GATGCTGCAA TTGGCACTAC CCCTTTCTCT 
ACTTGCTCGG TGGGGACTTG GTTTACTCCT TCAGCACCAC AGGAAAAGAG TACAAACACA 
TCCCAGACAG GCCTGGTTGG CACCAAGCAC AGTACTTCTG AGACAGAGCA GCTCCTGTGT 
GGCCGGCCTC CAGATCTGAC TGCCTTGTCT CGACATGACT TGGAAGATAA CCTGCTGAGC 
TCTCTTGTCA TTCTGGAGGT TCTCTCCCGC CAGCTTCGGG ACTGGAAGAG CCAGCTGGCT 
GTCCCTCACC CAGAAACCCA GGACAGTAGC ACACAGACTG ACACATCTCA CAGTGGGATA 
ACTAATAAAC TTCAGCATCT TAAGGAGAGC CATGAGATGG GACAGGCCCT ACAGCAGGCC 
AGAAATGTCA TGCAATCATG GGTGCTTATC TCTAAAGAGC TGATATCCTT GCTTCACCTA 
TCCCTGTTGC ATTTAGAAGA AGATAAGACT ACTGTGAGTC AGGAGTCTCG GCGTGCAGAA 
ACATTGGTCT GTTGCTGTTT TGATTTGCTG AAGAAATTGA GGGCAAAGCT CCAGAGCCTC 
AAAGCAGAAA GGGAGGAGGC AAGGCACAGA GAGGAAATGG CTCTCAGAGG CAAGGATGCG 
GCAGAGATAG TGTTGGAGGC TTTCTGTGCA CACGCCAGCC AGCGCATCAG CCAGCTGGAA 
CAGGACCTAG CATCCATGCG GGAATTCAGA GGCCTTCTGA AGGATGCCCA GACCCAACTG 
GTAGGGCTTC ATGCCAAGCA AGAAGAGCTG GTTCAGCAGA CAGTGAGTCT TACTTCTACC 
TTGCAACAAG ACTGGAGGTC CATGCAACTG GATTATACAA CATGGACAGC TTTGCTGAGT 
CGGTCCCGAC AACTCACAGA GAAACTCACA GTCAAGAGCC AGCAAGCCCT GCAGGAACGT 
GATGTGGCAA TTGAGGAAAA GCAGGAGGTT TCTAGGGTGC TGGAACAAGT CTCTGCCCAG 
TTAGAGGAGT GCAAAGGCCA AACAGAACAA CTGGAGTTGG AAAACAGTCG TCTAGCAACA 
GATCTCCGGG CTCAGTTGCA GATTCTGGCC AACATGGACA GCCAGCTAAA AGAGCTACAG 
AGTCAGCATA CCCATTGTGC CCAGGACCTG GCTATGAAGG ATGAGTTACT CTGCCAGCTT 
ACCCAGAGCA ATGAGGAGCA GGCTGCTCAA TGGCAAAAGG AAGAGATGGC ACTAAAACAC 
ATGCAGGCAG AACTGCAGCA GCAACAAGCT GTCCTGGCCA AAGAGGTGCG GGACCTGAAA 
GAGACCTTGG AGTTTGCAGA CCAGGAGAAT CAGGTTGCTC ACCTGGAGCT GGGTCAGGTT 
GAGTGTCAAT TGAAAACCAC ACTGGAAGTG CTCCGGGAGC GCAGCTTGCA GTGTGAGAAC 
CTCAAGGACA CTGTAGAGAA CCTAACGGCT AAACTGGCCA GCACCATAGC AGATAACCAG 
GAGCAAGATC TGGAGAAAAC ACGGCAGTAC TCTCAAAAGC TAGGGCTGCT GACTGAGCAA 
CTACAGAGCC TGACTCTCTT TCTACAGACA AAACTAAAGG AGAAGACTGA ACAAGAGACC 
CTTCTGCTGA GTACAGCCTG TCCTCCCACC CAGGAACACC CTCTGCCTAA TGACAGGACC 
TTCCTGGGAA GCATCTTGAC AGCAGTGGCA GATGAAGAGC CAGAATCAAC TCCTGTGCCC 
TTGCTTGGAA GTGACAAGAG TGCTTTCACC CGAGTAGCAT CAATGGTTTC CCTTCAGCCC 
GCAGAGACCC CAGGCATGGA GGAGAGCCTG GCAGAAATGA GTATTATGAC TACTGAGCTT 
CAGAGTCTTT GTTCCCTGCT ACAAGAGTCT AAAGAAGAAG CCATCAGGAC TCTGCAGCGA 
AAAATTTGTG AGCTGCAAGC TAGGCTGCAG GCCCAGGAAG AACAGCATCA GGAAGTCCAG 
AAGGCAAAAG AAGCAGACAT AGAGAAGCTG AACCAGGCCT TGTGCTTGCG CTACAAGAAT 
GAAAAGGAGC TCCAGGAAGT GATACAGCAG CAGAATGAGA AGATCCTAGA ACAGATAGAC 
AAGAGTGGCG AGCTCATAAG CCTTAGAGAG GAGGTGACCC ACCTTACCCG CTCACTTCGG 
CGTGCGGAGA CAGAGACCAA AGTGCTCCAG GAGGCCCTGG CAGGCCAGCT GGACTCCAAC 
TGCCAGCCTA TGGCCACCAA TTGGATCCAG GAGAAAGTGT GGCTCTCTCA GGAGGTGGAC 
AAACTGAGAG TGATGTTCCT GGAGATGAAA AATGAGAAGG AAAAACTCAT GATCAAGTTC 
CAGAGCCATA GAAATATCCT AGAGGAGAAC CTTCGGCGCT CTGACAAGGA GTTAGAAAAA 
CTAGATGACA TTGTTCAGCA TATTTATAAG ACCCTGCTCT CTATTCCAGA GGTGGTGAGG 
GGATGCAAAG AACTACAGGG ATTGCTGGAA TTTCTGAGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_006452.3
CDS	80..3661
Translation	MWRVKKLSLSLSPSPQTGKPSMRTPLRELTLQPGALTNSGKRSPACSSLTPSLCKLGLQEGSNNSSPVDFVNNKRTDLSSEHFSHSSKWLETCQHESDEQPLDPIPQISSTPKTSEEAVDPLGNYMVKTIVLVPSPLGQQQDMIFEARLDTMAETNSISLNGPLRTDDLVREEVAPCMGDRFSEVAAVSEKPIFQESPSHLLEESPPNPCSEQLHCSKESLSSRTEAVREDLVPSESNAFLPSSVLWLSPSTALAADFRVNHVDPEEEIVEHGAMEEREMRFPTHPKESETEDQALVSSVEDILSTCLTPNLVEMESQEAPGPAVEDVGRILGSDTESWMSPLAWLEKGVNTSVMLENLRQSLSLPSMLRDAAIGTTPFSTCSVGTWFTPSAPQEKSTNTSQTGLVGTKHSTSETEQLLCGRPPDLTALSRHDLEDNLLSSLVILEVLSRQLRDWKSQLAVPHPETQDSSTQTDTSHSGITNKLQHLKESHEMGQALQQARNVMQSWVLISKELISLLHLSLLHLEEDKTTVSQESRRAETLVCCCFDLLKKLRAKLQSLKAEREEARHREEMALRGKDAAEIVLEAFCAHASQRISQLEQDLASMREFRGLLKDAQTQLVGLHAKQEELVQQTVSLTSTLQQDWRSMQLDYTTWTALLSRSRQLTEKLTVKSQQALQERDVAIEEKQEVSRVLEQVSAQLEECKGQTEQLELENSRLATDLRAQLQILANMDSQLKELQSQHTHCAQDLAMKDELLCQLTQSNEEQAAQWQKEEMALKHMQAELQQQQAVLAKEVRDLKETLEFADQENQVAHLELGQVECQLKTTLEVLRERSLQCENLKDTVENLTAKLASTIADNQEQDLEKTRQYSQKLGLLTEQLQSLTLFLQTKLKEKTEQETLLLSTACPPTQEHPLPNDRTFLGSILTAVADEEPESTPVPLLGSDKSAFTRVASMVSLQPAETPGMEESLAEMSIMTTELQSLCSLLQESKEEAIRTLQRKICELQARLQAQEEQHQEVQKAKEADIEKLNQALCLRYKNEKELQEVIQQQNEKILEQIDKSGELISLREEVTHLTRSLRRAETETKVLQEALAGQLDSNCQPMATNWIQEKVWLSQEVDKLRVMFLEMKNEKEKLMIKFQSHRNILEENLRRSDKELEKLDDIVQHIYKTLLSIPEVVRGCKELQGLLEFLS

Target ORF information:

RefSeq Version	NM_006461.4
Organism	Homo sapiens(human)
Definition	Homo sapiens sperm associated antigen 5 (SPAG5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006461.4

ORF Insert Sequence:

ATGTGGCGAG TGAAAAAACT GAGCCTCAGC CTGTCGCCTT CGCCCCAGAC GGGAAAACCA 
TCTATGAGAA CTCCTCTCCG TGAACTTACC CTGCAGCCCG GTGCCCTCAC CAACTCTGGA 
AAAAGATCCC CCGCTTGCTC CTCGCTGACC CCATCACTGT GCAAGCTGGG GCTGCAGGAA 
GGCAGCAACA ACTCATCTCC AGTGGATTTT GTAAATAACA AGAGGACAGA CTTATCTTCA 
GAACATTTCA GTCATTCCTC AAAGTGGCTA GAAACTTGTC AGCATGAATC AGATGAGCAG 
CCTCTAGATC CAATTCCCCA AATTAGCTCT ACTCCTAAAA CGTCTGAGGA AGCAGTAGAC 
CCACTGGGCA ATTATATGGT TAAAACCATC GTCCTTGTAC CATCTCCACT GGGGCAGCAA 
CAAGACATGA TATTTGAGGC CCGTTTAGAT ACCATGGCAG AGACAAACAG CATATCTTTA 
AATGGACCTT TGAGAACAGA CGATCTGGTG AGAGAGGAGG TGGCACCCTG CATGGGAGAC 
AGGTTTTCAG AAGTTGCTGC TGTATCTGAG AAACCTATCT TTCAGGAATC TCCGTCCCAT 
CTCTTAGAGG AGTCTCCACC AAATCCCTGT TCTGAACAAC TACATTGCTC CAAGGAAAGC 
CTGAGCAGTA GAACTGAGGC TGTGCGTGAG GACTTAGTAC CTTCTGAAAG TAACGCCTTC 
TTGCCTTCCT CTGTTCTCTG GCTTTCCCCT TCAACTGCCT TGGCAGCAGA TTTCCGTGTC 
AATCATGTGG ACCCAGAGGA GGAAATTGTA GAGCATGGAG CTATGGAGGA AAGAGAAATG 
AGGTTTCCCA CACATCCTAA GGAGTCTGAA ACAGAAGATC AAGCACTTGT CTCAAGTGTG 
GAAGATATTC TGTCCACATG CCTGACACCA AATCTAGTAG AAATGGAATC CCAAGAAGCT 
CCAGGCCCAG CAGTAGAAGA TGTTGGTAGG ATTCTTGGCT CTGATACAGA GTCTTGGATG 
TCCCCACTGG CCTGGCTGGA AAAAGGTGTA AATACCTCCG TCATGCTGGA AAATCTCCGC 
CAAAGCTTAT CCCTTCCCTC GATGCTTCGG GATGCTGCAA TTGGCACTAC CCCTTTCTCT 
ACTTGCTCGG TGGGGACTTG GTTTACTCCT TCAGCACCAC AGGAAAAGAG TACAAACACA 
TCCCAGACAG GCCTGGTTGG CACCAAGCAC AGTACTTCTG AGACAGAGCA GCTCCTGTGT 
GGCCGGCCTC CAGATCTGAC TGCCTTGTCT CGACATGACT TGGAAGATAA CCTGCTGAGC 
TCTCTTGTCA TTCTGGAGGT TCTCTCCCGC CAGCTTCGGG ACTGGAAGAG CCAGCTGGCT 
GTCCCTCACC CAGAAACCCA GGACAGTAGC ACACAGACTG ACACATCTCA CAGTGGGATA 
ACTAATAAAC TTCAGCATCT TAAGGAGAGC CATGAGATGG GACAGGCCCT ACAGCAGGCC 
AGAAATGTCA TGCAATCATG GGTGCTTATC TCTAAAGAGC TGATATCCTT GCTTCACCTA 
TCCCTGTTGC ATTTAGAAGA AGATAAGACT ACTGTGAGTC AGGAGTCTCG GCGTGCAGAA 
ACATTGGTCT GTTGCTGTTT TGATTTGCTG AAGAAATTGA GGGCAAAGCT CCAGAGCCTC 
AAAGCAGAAA GGGAGGAGGC AAGGCACAGA GAGGAAATGG CTCTCAGAGG CAAGGATGCG 
GCAGAGATAG TGTTGGAGGC TTTCTGTGCA CACGCCAGCC AGCGCATCAG CCAGCTGGAA 
CAGGACCTAG CATCCATGCG GGAATTCAGA GGCCTTCTGA AGGATGCCCA GACCCAACTG 
GTAGGGCTTC ATGCCAAGCA AGAAGAGCTG GTTCAGCAGA CAGTGAGTCT TACTTCTACC 
TTGCAACAAG ACTGGAGGTC CATGCAACTG GATTATACAA CATGGACAGC TTTGCTGAGT 
CGGTCCCGAC AACTCACAGA GAAACTCACA GTCAAGAGCC AGCAAGCCCT GCAGGAACGT 
GATGTGGCAA TTGAGGAAAA GCAGGAGGTT TCTAGGGTGC TGGAACAAGT CTCTGCCCAG 
TTAGAGGAGT GCAAAGGCCA AACAGAACAA CTGGAGTTGG AAAACAGTCG TCTAGCAACA 
GATCTCCGGG CTCAGTTGCA GATTCTGGCC AACATGGACA GCCAGCTAAA AGAGCTACAG 
AGTCAGCATA CCCATTGTGC CCAGGACCTG GCTATGAAGG ATGAGTTACT CTGCCAGCTT 
ACCCAGAGCA ATGAGGAGCA GGCTGCTCAA TGGCAAAAGG AAGAGATGGC ACTAAAACAC 
ATGCAGGCAG AACTGCAGCA GCAACAAGCT GTCCTGGCCA AAGAGGTGCG GGACCTGAAA 
GAGACCTTGG AGTTTGCAGA CCAGGAGAAT CAGGTTGCTC ACCTGGAGCT GGGTCAGGTT 
GAGTGTCAAT TGAAAACCAC ACTGGAAGTG CTCCGGGAGC GCAGCTTGCA GTGTGAGAAC 
CTCAAGGACA CTGTAGAGAA CCTAACGGCT AAACTGGCCA GCACCATAGC AGATAACCAG 
GAGCAAGATC TGGAGAAAAC ACGGCAGTAC TCTCAAAAGC TAGGGCTGCT GACTGAGCAA 
CTACAGAGCC TGACTCTCTT TCTACAGACA AAACTAAAGG AGAAGACTGA ACAAGAGACC 
CTTCTGCTGA GTACAGCCTG TCCTCCCACC CAGGAACACC CTCTGCCTAA TGACAGGACC 
TTCCTGGGAA GCATCTTGAC AGCAGTGGCA GATGAAGAGC CAGAATCAAC TCCTGTGCCC 
TTGCTTGGAA GTGACAAGAG TGCTTTCACC CGAGTAGCAT CAATGGTTTC CCTTCAGCCC 
GCAGAGACCC CAGGCATGGA GGAGAGCCTG GCAGAAATGA GTATTATGAC TACTGAGCTT 
CAGAGTCTTT GTTCCCTGCT ACAAGAGTCT AAAGAAGAAG CCATCAGGAC TCTGCAGCGA 
AAAATTTGTG AGCTGCAAGC TAGGCTGCAG GCCCAGGAAG AACAGCATCA GGAAGTCCAG 
AAGGCAAAAG AAGCAGACAT AGAGAAGCTG AACCAGGCCT TGTGCTTGCG CTACAAGAAT 
GAAAAGGAGC TCCAGGAAGT GATACAGCAG CAGAATGAGA AGATCCTAGA ACAGATAGAC 
AAGAGTGGCG AGCTCATAAG CCTTAGAGAG GAGGTGACCC ACCTTACCCG CTCACTTCGG 
CGTGCGGAGA CAGAGACCAA AGTGCTCCAG GAGGCCCTGG CAGGCCAGCT GGACTCCAAC 
TGCCAGCCTA TGGCCACCAA TTGGATCCAG GAGAAAGTGT GGCTCTCTCA GGAGGTGGAC 
AAACTGAGAG TGATGTTCCT GGAGATGAAA AATGAGAAGG AAAAACTCAT GATCAAGTTC 
CAGAGCCATA GAAATATCCT AGAGGAGAAC CTTCGGCGCT CTGACAAGGA GTTAGAAAAA 
CTAGATGACA TTGTTCAGCA TATTTATAAG ACCCTGCTCT CTATTCCAGA GGTGGTGAGG 
GGATGCAAAG AACTACAGGG ATTGCTGGAA TTTCTGAGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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SPAG5 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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