AHSA1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	AHSA1
Entrez Gene ID	10598
Full Name	activator of HSP90 ATPase activity 1
Synonyms	AHA1,C14orf3,hAha1,p38
General protein information	Preferred Names activator of HSP90 ATPase activity 1 Names activator of 90 kDa heat shock protein ATPase homolog 1 AHA1, activator of heat shock 90kDa protein ATPase homolog 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 14 Map Location: 14q24.3
Disorder MIM:	608466

mRNA and Protein(s)

mRNA	Protein	Name
NM_012111.2	NP_036243.1	activator of 90 kDa heat shock protein ATPase homolog 1 isoform 1
NM_001321441.1	NP_001308370.1	activator of 90 kDa heat shock protein ATPase homolog 1 isoform 2
NM_001321441.2	NP_001308370.1	activator of 90 kDa heat shock protein ATPase homolog 1 isoform 2
NM_012111.3	NP_036243.1	activator of 90 kDa heat shock protein ATPase homolog 1 isoform 1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	AHSA1	NP_001029838.1
Mus musculus (house mouse)	Ahsa1	NP_666148.1
Danio rerio (zebrafish)	ahsa1l	NP_997767.1
Oryza sativa (rice)	Os08g0464000	NP_001061992.1
Homo sapiens (human)	AHSA1	NP_036243.1
Pan troglodytes (chimpanzee)	AHSA1	XP_001165345.1
Gallus gallus (chicken)	AHSA1	XP_421292.1
Caenorhabditis elegans (roundworm)	CELE_C01G10.8	NP_506715.1
Schizosaccharomyces pombe (fission yeast)	SPBC1711.08	NP_595881.1
Neurospora crassa	NCU04087	XP_956083.1
Xenopus tropicalis (tropical clawed frog)	ahsa1	NP_001008135.2
Eremothecium gossypii	AGOS_AGL090W	NP_986576.1
Drosophila melanogaster (fruit fly)	CG1416	NP_610121.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010514	XP_314487.3
Saccharomyces cerevisiae (baker's yeast)	AHA1	NP_010500.3
Magnaporthe oryzae (rice blast fungus)	MGG_00814	XP_003718169.1
	AHSA1	XP_001103794.1
Canis lupus familiaris (dog)	AHSA1	XP_537523.2
Rattus norvegicus (Norway rat)	Ahsa1	NP_001108506.1
Kluyveromyces lactis	KLLA0A04147g	XP_451183.1
Arabidopsis thaliana (thale cress)	AT3G12050	NP_566410.1

Gene Ontology
Bibliography

Related articles in PubMed

Tumor suppressor Tsc1 is a new Hsp90 co-chaperone that facilitates folding of kinase and non-kinase clients.
Woodford MR, Sager RA, Marris E, Dunn DM, Blanden AR, Murphy RL, Rensing N, Shapiro O, Panaretou B, Prodromou C, Loh SN, Gutmann DH, Bourboulia D, Bratslavsky G, Wong M, Mollapour M
The EMBO journal36(24)3650-3665(2017 12)

Hsp90 activator Aha1 drives production of pathological tau aggregates.
Shelton LB, Baker JD, Zheng D, Sullivan LE, Solanki PK, Webster JM, Sun Z, Sabbagh JJ, Nordhues BA, Koren J 3rd, Ghosh S, Blagg BSJ, Blair LJ, Dickey CA
Proceedings of the National Academy of Sciences of the United States of America114(36)9707-9712(2017 Sep)

The FNIP co-chaperones decelerate the Hsp90 chaperone cycle and enhance drug binding.
Woodford MR, Dunn DM, Blanden AR, Capriotti D, Loiselle D, Prodromou C, Panaretou B, Hughes PF, Smith A, Ackerman W, Haystead TA, Loh SN, Bourboulia D, Schmidt LS, Marston Linehan W, Bratslavsky G, Mollapour M
Nature communications712037(2016 Jun)

AHSA1 regulates proliferation, apoptosis, migration, and invasion of osteosarcoma.
Shao J, Wang L, Zhong C, Qi R, Li Y
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie7745-51(2016 Feb)

Middle domain of human Hsp90 isoforms differentially binds Aha1 in human cells and alters Hsp90 activity in yeast.
Synoradzki K, Bieganowski P
Biochimica et biophysica acta1853(2)445-52(2015 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These results suggest that differences in the middle domain of Hsp90alpha and Hsp90beta may be responsible for the isoform-specific interactions with selected proteins.
Title: Middle domain of human Hsp90 isoforms differentially binds Aha1 in human cells and alters Hsp90 activity in yeast.

Aha1 may promote disposal of folding defective proteins by the cellular protein quality control.
Title: Aha1 can act as an autonomous chaperone to prevent aggregation of stressed proteins.

a monoallelic mutation of p53 was sufficient to activate the Aha1/Hsp90 ATPase axis leading to stimulation of Wnt signaling and increased expression of Wnt target genes.
Title: p53 protein regulates Hsp90 ATPase activity and thereby Wnt signaling by modulating Aha1 expression.

Modulation of Hsp90 activity by AHA1 regulates VEGF signaling to eNOS and angiogenesis.
Title: Modulation of the cochaperone AHA1 regulates heat-shock protein 90 and endothelial NO synthase activation by vascular endothelial growth factor.

Hsp90 phosphorylation on tyrosine313 promotes recruitment of AHA1, which stimulates Hsp90 ATPase activity, furthering the chaperoning process.
Title: Dynamic tyrosine phosphorylation modulates cycling of the HSP90-P50(CDC37)-AHA1 chaperone machine.

The following AHSA1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AHSA1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu09158	NM_012111.3 Latest version!	Homo sapiens activator of HSP90 ATPase activity 1 (AHSA1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu64178	NM_001321441.2 Latest version!	Homo sapiens activator of HSP90 ATPase activity 1 (AHSA1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
View Old Accession Versions >>
OHu64178	NM_001321441.1	Homo sapiens activator of HSP90 ATPase activity 1 (AHSA1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
OHu09158	NM_012111.2	Homo sapiens activator of HSP90 ATPase activity 1 (AHSA1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AHSA1 ( NM_012111.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_036243.1 Homo sapiens activator of HSP90 ATPase activity 1 (AHSA1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu09158
Clone ID Related Accession (Same CDS sequence)	NM_012111.2 , NM_012111.3
Accession Version	NM_012111.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1017bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	activator of 90 kDa heat shock protein ATPase homolog 1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BG432594.1, BC000321.2 and AF161440.1. On Feb 28, 2009 this sequence version replaced NM_012111.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163658.122003.1, SRR1163657.262141.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCAAGT GGGGTGAGGG AGACCCACGC TGGATCGTGG AGGAGCGGGC GGACGCCACC 
AACGTCAACA ACTGGCACTG GACGGAGAGA GATGCTTCAA ATTGGTCCAC GGATAAGCTG 
AAAACACTGT TCCTGGCAGT GCAGGTTCAA AATGAAGAAG GCAAGTGTGA GGTGACGGAA 
GTGAGTAAGC TTGATGGAGA GGCATCCATT AACAATCGCA AAGGGAAACT TATCTTCTTT 
TATGAATGGA GCGTCAAACT AAACTGGACA GGTACTTCTA AGTCAGGAGT ACAATACAAA 
GGACATGTGG AGATCCCCAA TTTGTCTGAT GAAAACAGCG TGGATGAAGT GGAGATTAGT 
GTGAGCCTTG CCAAAGATGA GCCTGACACA AATCTCGTGG CCTTAATGAA GGAAGAAGGG 
GTGAAACTTC TAAGAGAAGC AATGGGAATT TACATCAGCA CCCTCAAAAC AGAGTTCACC 
CAGGGCATGA TCTTACCTAC AATGAATGGA GAGTCAGTAG ACCCAGTGGG GCAGCCAGCA 
CTGAAAACTG AGGAGCGCAA GGCTAAGCCT GCTCCTTCAA AAACCCAGGC CAGACCTGTT 
GGAGTCAAAA TCCCCACTTG TAAGATCACT CTTAAGGAAA CCTTCCTGAC GTCACCAGAG 
GAGCTCTATA GAGTGTTTAC CACCCAAGAG CTGGTGCAGG CCTTTACCCA TGCTCCTGCA 
ACATTAGAAG CAGACAGAGG TGGAAAGTTC CACATGGTAG ATGGCAACGT CTCTGGGGAA 
TTTACTGATC TGGTCCCTGA GAAACATATT GTGATGAAGT GGAGGTTTAA ATCTTGGCCA 
GAGGGACACT TTGCCACCAT CACCTTGACC TTCATCGACA AGAACGGAGA GACTGAGCTG 
TGCATGGAAG GTCGAGGCAT CCCTGCTCCT GAGGAAGAGC GGACGCGACA GGGCTGGCAG 
CGGTACTACT TTGAGGGCAT TAAACAGACC TTTGGCTATG GCGCACGCTT ATTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036243.1
CDS	161..1177
Translation	MAKWGEGDPRWIVEERADATNVNNWHWTERDASNWSTDKLKTLFLAVQVQNEEGKCEVTEVSKLDGEASINNRKGKLIFFYEWSVKLNWTGTSKSGVQYKGHVEIPNLSDENSVDEVEISVSLAKDEPDTNLVALMKEEGVKLLREAMGIYISTLKTEFTQGMILPTMNGESVDPVGQPALKTEERKAKPAPSKTQARPVGVKIPTCKITLKETFLTSPEELYRVFTTQELVQAFTHAPATLEADRGGKFHMVDGNVSGEFTDLVPEKHIVMKWRFKSWPEGHFATITLTFIDKNGETELCMEGRGIPAPEEERTRQGWQRYYFEGIKQTFGYGARLF

Target ORF information:

RefSeq Version	NM_012111.2
Organism	Homo sapiens(human)
Definition	Homo sapiens activator of HSP90 ATPase activity 1 (AHSA1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012111.2

ORF Insert Sequence:

ATGGCCAAGT GGGGTGAGGG AGACCCACGC TGGATCGTGG AGGAGCGGGC GGACGCCACC 
AACGTCAACA ACTGGCACTG GACGGAGAGA GATGCTTCAA ATTGGTCCAC GGATAAGCTG 
AAAACACTGT TCCTGGCAGT GCAGGTTCAA AATGAAGAAG GCAAGTGTGA GGTGACGGAA 
GTGAGTAAGC TTGATGGAGA GGCATCCATT AACAATCGCA AAGGGAAACT TATCTTCTTT 
TATGAATGGA GCGTCAAACT AAACTGGACA GGTACTTCTA AGTCAGGAGT ACAATACAAA 
GGACATGTGG AGATCCCCAA TTTGTCTGAT GAAAACAGCG TGGATGAAGT GGAGATTAGT 
GTGAGCCTTG CCAAAGATGA GCCTGACACA AATCTCGTGG CCTTAATGAA GGAAGAAGGG 
GTGAAACTTC TAAGAGAAGC AATGGGAATT TACATCAGCA CCCTCAAAAC AGAGTTCACC 
CAGGGCATGA TCTTACCTAC AATGAATGGA GAGTCAGTAG ACCCAGTGGG GCAGCCAGCA 
CTGAAAACTG AGGAGCGCAA GGCTAAGCCT GCTCCTTCAA AAACCCAGGC CAGACCTGTT 
GGAGTCAAAA TCCCCACTTG TAAGATCACT CTTAAGGAAA CCTTCCTGAC GTCACCAGAG 
GAGCTCTATA GAGTGTTTAC CACCCAAGAG CTGGTGCAGG CCTTTACCCA TGCTCCTGCA 
ACATTAGAAG CAGACAGAGG TGGAAAGTTC CACATGGTAG ATGGCAACGT CTCTGGGGAA 
TTTACTGATC TGGTCCCTGA GAAACATATT GTGATGAAGT GGAGGTTTAA ATCTTGGCCA 
GAGGGACACT TTGCCACCAT CACCTTGACC TTCATCGACA AGAACGGAGA GACTGAGCTG 
TGCATGGAAG GTCGAGGCAT CCCTGCTCCT GAGGAAGAGC GGACGCGACA GGGCTGGCAG 
CGGTACTACT TTGAGGGCAT TAAACAGACC TTTGGCTATG GCGCACGCTT ATTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AHSA1 ( NM_012111.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_036243.1 Homo sapiens activator of HSP90 ATPase activity 1 (AHSA1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu09158
Clone ID Related Accession (Same CDS sequence)	NM_012111.2 , NM_012111.3
Accession Version	NM_012111.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1017bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-25
Organism	Homo sapiens(human)
Product	activator of 90 kDa heat shock protein ATPase homolog 1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC000321.2 and AF161440.1. On Nov 22, 2018 this sequence version replaced NM_012111.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AJ243310.1, SRR1163657.47594.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000216479.8/ ENSP00000216479.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCCAAGT GGGGTGAGGG AGACCCACGC TGGATCGTGG AGGAGCGGGC GGACGCCACC 
AACGTCAACA ACTGGCACTG GACGGAGAGA GATGCTTCAA ATTGGTCCAC GGATAAGCTG 
AAAACACTGT TCCTGGCAGT GCAGGTTCAA AATGAAGAAG GCAAGTGTGA GGTGACGGAA 
GTGAGTAAGC TTGATGGAGA GGCATCCATT AACAATCGCA AAGGGAAACT TATCTTCTTT 
TATGAATGGA GCGTCAAACT AAACTGGACA GGTACTTCTA AGTCAGGAGT ACAATACAAA 
GGACATGTGG AGATCCCCAA TTTGTCTGAT GAAAACAGCG TGGATGAAGT GGAGATTAGT 
GTGAGCCTTG CCAAAGATGA GCCTGACACA AATCTCGTGG CCTTAATGAA GGAAGAAGGG 
GTGAAACTTC TAAGAGAAGC AATGGGAATT TACATCAGCA CCCTCAAAAC AGAGTTCACC 
CAGGGCATGA TCTTACCTAC AATGAATGGA GAGTCAGTAG ACCCAGTGGG GCAGCCAGCA 
CTGAAAACTG AGGAGCGCAA GGCTAAGCCT GCTCCTTCAA AAACCCAGGC CAGACCTGTT 
GGAGTCAAAA TCCCCACTTG TAAGATCACT CTTAAGGAAA CCTTCCTGAC GTCACCAGAG 
GAGCTCTATA GAGTGTTTAC CACCCAAGAG CTGGTGCAGG CCTTTACCCA TGCTCCTGCA 
ACATTAGAAG CAGACAGAGG TGGAAAGTTC CACATGGTAG ATGGCAACGT CTCTGGGGAA 
TTTACTGATC TGGTCCCTGA GAAACATATT GTGATGAAGT GGAGGTTTAA ATCTTGGCCA 
GAGGGACACT TTGCCACCAT CACCTTGACC TTCATCGACA AGAACGGAGA GACTGAGCTG 
TGCATGGAAG GTCGAGGCAT CCCTGCTCCT GAGGAAGAGC GGACGCGACA GGGCTGGCAG 
CGGTACTACT TTGAGGGCAT TAAACAGACC TTTGGCTATG GCGCACGCTT ATTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036243.1
CDS	98..1114
Translation	MAKWGEGDPRWIVEERADATNVNNWHWTERDASNWSTDKLKTLFLAVQVQNEEGKCEVTEVSKLDGEASINNRKGKLIFFYEWSVKLNWTGTSKSGVQYKGHVEIPNLSDENSVDEVEISVSLAKDEPDTNLVALMKEEGVKLLREAMGIYISTLKTEFTQGMILPTMNGESVDPVGQPALKTEERKAKPAPSKTQARPVGVKIPTCKITLKETFLTSPEELYRVFTTQELVQAFTHAPATLEADRGGKFHMVDGNVSGEFTDLVPEKHIVMKWRFKSWPEGHFATITLTFIDKNGETELCMEGRGIPAPEEERTRQGWQRYYFEGIKQTFGYGARLF

Target ORF information:

RefSeq Version	NM_012111.3
Organism	Homo sapiens(human)
Definition	Homo sapiens activator of HSP90 ATPase activity 1 (AHSA1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012111.3

ORF Insert Sequence:

ATGGCCAAGT GGGGTGAGGG AGACCCACGC TGGATCGTGG AGGAGCGGGC GGACGCCACC 
AACGTCAACA ACTGGCACTG GACGGAGAGA GATGCTTCAA ATTGGTCCAC GGATAAGCTG 
AAAACACTGT TCCTGGCAGT GCAGGTTCAA AATGAAGAAG GCAAGTGTGA GGTGACGGAA 
GTGAGTAAGC TTGATGGAGA GGCATCCATT AACAATCGCA AAGGGAAACT TATCTTCTTT 
TATGAATGGA GCGTCAAACT AAACTGGACA GGTACTTCTA AGTCAGGAGT ACAATACAAA 
GGACATGTGG AGATCCCCAA TTTGTCTGAT GAAAACAGCG TGGATGAAGT GGAGATTAGT 
GTGAGCCTTG CCAAAGATGA GCCTGACACA AATCTCGTGG CCTTAATGAA GGAAGAAGGG 
GTGAAACTTC TAAGAGAAGC AATGGGAATT TACATCAGCA CCCTCAAAAC AGAGTTCACC 
CAGGGCATGA TCTTACCTAC AATGAATGGA GAGTCAGTAG ACCCAGTGGG GCAGCCAGCA 
CTGAAAACTG AGGAGCGCAA GGCTAAGCCT GCTCCTTCAA AAACCCAGGC CAGACCTGTT 
GGAGTCAAAA TCCCCACTTG TAAGATCACT CTTAAGGAAA CCTTCCTGAC GTCACCAGAG 
GAGCTCTATA GAGTGTTTAC CACCCAAGAG CTGGTGCAGG CCTTTACCCA TGCTCCTGCA 
ACATTAGAAG CAGACAGAGG TGGAAAGTTC CACATGGTAG ATGGCAACGT CTCTGGGGAA 
TTTACTGATC TGGTCCCTGA GAAACATATT GTGATGAAGT GGAGGTTTAA ATCTTGGCCA 
GAGGGACACT TTGCCACCAT CACCTTGACC TTCATCGACA AGAACGGAGA GACTGAGCTG 
TGCATGGAAG GTCGAGGCAT CCCTGCTCCT GAGGAAGAGC GGACGCGACA GGGCTGGCAG 
CGGTACTACT TTGAGGGCAT TAAACAGACC TTTGGCTATG GCGCACGCTT ATTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AHSA1 ( NM_001321441.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001308370.1 Homo sapiens activator of HSP90 ATPase activity 1 (AHSA1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu64178
Clone ID Related Accession (Same CDS sequence)	NM_001321441.1 , NM_001321441.2
Accession Version	NM_001321441.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 612bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-09-28
Organism	Homo sapiens(human)
Product	activator of 90 kDa heat shock protein ATPase homolog 1 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CX786861.1, BQ073652.1, BC000321.2 and AF161440.1. On Mar 25, 2016 this sequence version replaced XM_011536361.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163658.58633.1, SRR1803613.482192.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAGGAAG AAGGGGTGAA ACTTCTAAGA GAAGCAATGG GAATTTACAT CAGCACCCTC 
AAAACAGAGT TCACCCAGGG CATGATCTTA CCTACAATGA ATGGAGAGTC AGTAGACCCA 
GTGGGGCAGC CAGCACTGAA AACTGAGGAG CGCAAGGCTA AGCCTGCTCC TTCAAAAACC 
CAGGCCAGAC CTGTTGGAGT CAAAATCCCC ACTTGTAAGA TCACTCTTAA GGAAACCTTC 
CTGACGTCAC CAGAGGAGCT CTATAGAGTG TTTACCACCC AAGAGCTGGT GCAGGCCTTT 
ACCCATGCTC CTGCAACATT AGAAGCAGAC AGAGGTGGAA AGTTCCACAT GGTAGATGGC 
AACGTCTCTG GGGAATTTAC TGATCTGGTC CCTGAGAAAC ATATTGTGAT GAAGTGGAGG 
TTTAAATCTT GGCCAGAGGG ACACTTTGCC ACCATCACCT TGACCTTCAT CGACAAGAAC 
GGAGAGACTG AGCTGTGCAT GGAAGGTCGA GGCATCCCTG CTCCTGAGGA AGAGCGGACG 
CGACAGGGCT GGCAGCGGTA CTACTTTGAG GGCATTAAAC AGACCTTTGG CTATGGCGCA 
CGCTTATTTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001308370.1
CDS	432..1043
Translation	MKEEGVKLLREAMGIYISTLKTEFTQGMILPTMNGESVDPVGQPALKTEERKAKPAPSKTQARPVGVKIPTCKITLKETFLTSPEELYRVFTTQELVQAFTHAPATLEADRGGKFHMVDGNVSGEFTDLVPEKHIVMKWRFKSWPEGHFATITLTFIDKNGETELCMEGRGIPAPEEERTRQGWQRYYFEGIKQTFGYGARLF

Target ORF information:

RefSeq Version	NM_001321441.1
Organism	Homo sapiens(human)
Definition	Homo sapiens activator of HSP90 ATPase activity 1 (AHSA1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001321441.1

ORF Insert Sequence:

ATGAAGGAAG AAGGGGTGAA ACTTCTAAGA GAAGCAATGG GAATTTACAT CAGCACCCTC 
AAAACAGAGT TCACCCAGGG CATGATCTTA CCTACAATGA ATGGAGAGTC AGTAGACCCA 
GTGGGGCAGC CAGCACTGAA AACTGAGGAG CGCAAGGCTA AGCCTGCTCC TTCAAAAACC 
CAGGCCAGAC CTGTTGGAGT CAAAATCCCC ACTTGTAAGA TCACTCTTAA GGAAACCTTC 
CTGACGTCAC CAGAGGAGCT CTATAGAGTG TTTACCACCC AAGAGCTGGT GCAGGCCTTT 
ACCCATGCTC CTGCAACATT AGAAGCAGAC AGAGGTGGAA AGTTCCACAT GGTAGATGGC 
AACGTCTCTG GGGAATTTAC TGATCTGGTC CCTGAGAAAC ATATTGTGAT GAAGTGGAGG 
TTTAAATCTT GGCCAGAGGG ACACTTTGCC ACCATCACCT TGACCTTCAT CGACAAGAAC 
GGAGAGACTG AGCTGTGCAT GGAAGGTCGA GGCATCCCTG CTCCTGAGGA AGAGCGGACG 
CGACAGGGCT GGCAGCGGTA CTACTTTGAG GGCATTAAAC AGACCTTTGG CTATGGCGCA 
CGCTTATTTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AHSA1 ( NM_001321441.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001308370.1 Homo sapiens activator of HSP90 ATPase activity 1 (AHSA1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu64178
Clone ID Related Accession (Same CDS sequence)	NM_001321441.1 , NM_001321441.2
Accession Version	NM_001321441.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 612bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-27
Organism	Homo sapiens(human)
Product	activator of 90 kDa heat shock protein ATPase homolog 1 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AF111168.2, CX786861.1, BQ073652.1, BC000321.2 and AF161440.1. On Jun 1, 2019 this sequence version replaced NM_001321441.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.482192.1, SRR1163658.58633.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAGGAAG AAGGGGTGAA ACTTCTAAGA GAAGCAATGG GAATTTACAT CAGCACCCTC 
AAAACAGAGT TCACCCAGGG CATGATCTTA CCTACAATGA ATGGAGAGTC AGTAGACCCA 
GTGGGGCAGC CAGCACTGAA AACTGAGGAG CGCAAGGCTA AGCCTGCTCC TTCAAAAACC 
CAGGCCAGAC CTGTTGGAGT CAAAATCCCC ACTTGTAAGA TCACTCTTAA GGAAACCTTC 
CTGACGTCAC CAGAGGAGCT CTATAGAGTG TTTACCACCC AAGAGCTGGT GCAGGCCTTT 
ACCCATGCTC CTGCAACATT AGAAGCAGAC AGAGGTGGAA AGTTCCACAT GGTAGATGGC 
AACGTCTCTG GGGAATTTAC TGATCTGGTC CCTGAGAAAC ATATTGTGAT GAAGTGGAGG 
TTTAAATCTT GGCCAGAGGG ACACTTTGCC ACCATCACCT TGACCTTCAT CGACAAGAAC 
GGAGAGACTG AGCTGTGCAT GGAAGGTCGA GGCATCCCTG CTCCTGAGGA AGAGCGGACG 
CGACAGGGCT GGCAGCGGTA CTACTTTGAG GGCATTAAAC AGACCTTTGG CTATGGCGCA 
CGCTTATTTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001308370.1
CDS	442..1053
Translation	MKEEGVKLLREAMGIYISTLKTEFTQGMILPTMNGESVDPVGQPALKTEERKAKPAPSKTQARPVGVKIPTCKITLKETFLTSPEELYRVFTTQELVQAFTHAPATLEADRGGKFHMVDGNVSGEFTDLVPEKHIVMKWRFKSWPEGHFATITLTFIDKNGETELCMEGRGIPAPEEERTRQGWQRYYFEGIKQTFGYGARLF

Target ORF information:

RefSeq Version	NM_001321441.2
Organism	Homo sapiens(human)
Definition	Homo sapiens activator of HSP90 ATPase activity 1 (AHSA1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001321441.2

ORF Insert Sequence:

ATGAAGGAAG AAGGGGTGAA ACTTCTAAGA GAAGCAATGG GAATTTACAT CAGCACCCTC 
AAAACAGAGT TCACCCAGGG CATGATCTTA CCTACAATGA ATGGAGAGTC AGTAGACCCA 
GTGGGGCAGC CAGCACTGAA AACTGAGGAG CGCAAGGCTA AGCCTGCTCC TTCAAAAACC 
CAGGCCAGAC CTGTTGGAGT CAAAATCCCC ACTTGTAAGA TCACTCTTAA GGAAACCTTC 
CTGACGTCAC CAGAGGAGCT CTATAGAGTG TTTACCACCC AAGAGCTGGT GCAGGCCTTT 
ACCCATGCTC CTGCAACATT AGAAGCAGAC AGAGGTGGAA AGTTCCACAT GGTAGATGGC 
AACGTCTCTG GGGAATTTAC TGATCTGGTC CCTGAGAAAC ATATTGTGAT GAAGTGGAGG 
TTTAAATCTT GGCCAGAGGG ACACTTTGCC ACCATCACCT TGACCTTCAT CGACAAGAAC 
GGAGAGACTG AGCTGTGCAT GGAAGGTCGA GGCATCCCTG CTCCTGAGGA AGAGCGGACG 
CGACAGGGCT GGCAGCGGTA CTACTTTGAG GGCATTAAAC AGACCTTTGG CTATGGCGCA 
CGCTTATTTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Tumor suppressor Tsc1 is a new Hsp90 co-chaperone that facilitates folding of kinase and non-kinase clients.
The EMBO journal36(24)3650-3665(2017 12)
Woodford MR,Sager RA,Marris E,Dunn DM,Blanden AR,Murphy RL,Rensing N,Shapiro O,Panaretou B,Prodromou C,Loh SN,Gutmann DH,Bourboulia D,Bratslavsky G,Wong M,Mollapour M

Hsp90 activator Aha1 drives production of pathological tau aggregates.
Proceedings of the National Academy of Sciences of the United States of America114(36)9707-9712(2017 Sep)
Shelton LB,Baker JD,Zheng D,Sullivan LE,Solanki PK,Webster JM,Sun Z,Sabbagh JJ,Nordhues BA,Koren J 3rd,Ghosh S,Blagg BSJ,Blair LJ,Dickey CA

The FNIP co-chaperones decelerate the Hsp90 chaperone cycle and enhance drug binding.
Nature communications712037(2016 Jun)
Woodford MR,Dunn DM,Blanden AR,Capriotti D,Loiselle D,Prodromou C,Panaretou B,Hughes PF,Smith A,Ackerman W,Haystead TA,Loh SN,Bourboulia D,Schmidt LS,Marston Linehan W,Bratslavsky G,Mollapour M

AHSA1 regulates proliferation, apoptosis, migration, and invasion of osteosarcoma.
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie7745-51(2016 Feb)
Shao J,Wang L,Zhong C,Qi R,Li Y

Middle domain of human Hsp90 isoforms differentially binds Aha1 in human cells and alters Hsp90 activity in yeast.
Biochimica et biophysica acta1853(2)445-52(2015 Feb)
Synoradzki K,Bieganowski P

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AHSA1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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