DRAP1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	DRAP1
Entrez Gene ID	10589
Full Name	DR1 associated protein 1
Synonyms	NC2-alpha
General protein information	Preferred Names DR1 associated protein 1 Names dr1-associated corepressor negative co-factor 2-alpha negative cofactor 2 alpha
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 11 Map Location: 11q13.1
Summary	Transcriptional repression is a general mechanism for regulating transcriptional initiation in organisms ranging from yeast to humans. Accurate initiation of transcription from eukaryotic protein-encoding genes requires the assembly of a large multiprotein complex consisting of RNA polymerase II and general transcription factors such as TFIIA, TFIIB, and TFIID. DR1 is a repressor that interacts with the TATA-binding protein (TBP) of TFIID and prevents the formation of an active transcription complex by precluding the entry of TFIIA and/or TFIIB into the preinitiation complex. The protein encoded by this gene is a corepressor of transcription that interacts with DR1 to enhance DR1-mediated repression. The interaction between this corepressor and DR1 is required for corepressor function and appears to stabilize the TBP-DR1-DNA complex. [provided by RefSeq, Jul 2008].
Disorder MIM:	602289

mRNA and Protein(s)

mRNA	Protein	Name
NM_006442.3	NP_006433.2	dr1-associated corepressor
NM_006442.4	NP_006433.2	dr1-associated corepressor

Pathways from BioSystems

Homologies

Homo sapiens (human)	DRAP1	NP_006433.2
Pan troglodytes (chimpanzee)	DRAP1	XP_003313186.2
Mus musculus (house mouse)	Drap1	NP_077138.1
Danio rerio (zebrafish)	drap1	NP_991104.1
Xenopus tropicalis (tropical clawed frog)	drap1	NP_989272.1
Canis lupus familiaris (dog)	DRAP1	XP_533227.3
Bos taurus (cattle)	DRAP1	NP_001069682.1
Rattus norvegicus (Norway rat)	Drap1	NP_001071136.1
	DRAP1	XP_001112037.1

Gene Ontology
Bibliography

Related articles in PubMed

Pooled-matrix protein interaction screens using Barcode Fusion Genetics.
Yachie N, Petsalaki E, Mellor JC, Weile J, Jacob Y, Verby M, Ozturk SB, Li S, Cote AG, Mosca R, Knapp JJ, Ko M, Yu A, Gebbia M, Sahni N, Yi S, Tyagi T, Sheykhkarimli D, Roth JF, Wong C, Musa L, Snider J, Liu YC, Yu H, Braun P, Stagljar I, Hao T, Calderwood MA, Pelletier L, Aloy P, Hill DE, Vidal M, Roth FP
Molecular systems biology12(4)863(2016 Apr)

Genome-wide association study identifies three novel susceptibility loci for severe Acne vulgaris.
Navarini AA, Simpson MA, Weale M, Knight J, Carlavan I, Reiniche P, Burden DA, Layton A, Bataille V, Allen M, Pleass R, Pink A, Creamer D, English J, Munn S, Walton S, , Willis C, D?ret S, Voegel JJ, Spector T, Smith CH, Trembath RC, Barker JN
Nature communications54020(2014 Jun)

Next-generation sequencing to generate interactome datasets.
Yu H, Tardivo L, Tam S, Weiner E, Gebreab F, Fan C, Svrzikapa N, Hirozane-Kishikawa T, Rietman E, Yang X, Sahalie J, Salehi-Ashtiani K, Hao T, Cusick ME, Hill DE, Roth FP, Braun P, Vidal M
Nature methods8(6)478-80(2011 Jun)

A census of human transcription factors: function, expression and evolution.
Vaquerizas JM, Kummerfeld SK, Teichmann SA, Luscombe NM
Nature reviews. Genetics10(4)252-63(2009 04)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

heterodimerization with NC2alpha masks the nuclear localization signal in NC2beta, which prevents nuclear export of the NC2 complex
Title: Regulation of nuclear import and export of negative cofactor 2.

provide evidence that negative cofactor-2 (NC2) induces dynamic conformational changes in the TBP-DNA complex that allow it to escape and return to TATA-binding mode
Title: NC2 mobilizes TBP on core promoter TATA boxes.

The global distribution of DRAP1 on promoters was determined.
Title: Global distribution of negative cofactor 2 subunit-alpha on human promoters.

physical cooperation between BTAF1 and NC2alpha in TBP regulation
Title: NC2alpha interacts with BTAF1 and stimulates its ATP-dependent association with TATA-binding protein.

Hypoxia actively represses transcription by inducing this protein and blocking preinitiation complex assembly.
Title: Hypoxia actively represses transcription by inducing negative cofactor 2 (Dr1/DrAP1) and blocking preinitiation complex assembly.

The following DRAP1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DRAP1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu55638	NM_006442.4 Latest version!	Homo sapiens DR1 associated protein 1 (DRAP1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
View Old Accession Versions >>
OHu55638	NM_006442.3	Homo sapiens DR1 associated protein 1 (DRAP1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DRAP1 ( NM_006442.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_006433.2 Homo sapiens DR1 associated protein 1 (DRAP1), mRNA.

Price & Availability↑

CloneID	OHu55638
Clone ID Related Accession (Same CDS sequence)	NM_006442.3 , NM_006442.4
Accession Version	NM_006442.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 618bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	dr1-associated corepressor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BG719065.1, BG036132.1, BC010025.2 and BQ773919.1. On Nov 6, 2009 this sequence version replaced NM_006442.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163655.676037.1, SRR1163657.265844.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCGAGCA AGAAGAAGAA GTACAACGCG CGGTTCCCGC CGGCGCGGAT CAAGAAGATC 
ATGCAGACGG ACGAAGAGAT TGGGAAGGTG GCGGCGGCGG TGCCTGTCAT CATCTCCCGG 
GCGCTCGAGC TCTTCCTAGA GTCGCTGTTG AAGAAGGCCT GCCAGGTGAC CCAGTCGCGG 
AACGCGAAGA CCATGACCAC ATCCCACCTG AAGCAGTGCA TCGAGCTGGA GCAGCAGTTT 
GACTTCTTGA AGGACCTGGT GGCATCTGTT CCCGACATGC AGGGGGACGG GGAAGACAAC 
CACATGGATG GGGACAAGGG CGCCCGCAGG GGCCGGAAGC CAGGCAGCGG CGGCCGGAAG 
AACGGTGGGA TGGGAACGAA AAGCAAGGAC AAGAAGCTGT CCGGGACAGA CTCGGAGCAG 
GAGGATGAAT CTGAGGACAC AGATACTGAT GGGGAAGAGG AGACATCACA ACCCCCACCC 
CAGGCCAGCC ACCCCTCTGC CCACTTTCAG AGCCCCCCGA CACCCTTCCT GCCCTTCGCC 
TCTACTCTGC CTTTGCCCCC AGCGCCCCCG GGCCCCTCAG CACCTGATGA AGAGGACGAA 
GAAGATTACG ACTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_006433.2
CDS	246..863
Translation	MPSKKKKYNARFPPARIKKIMQTDEEIGKVAAAVPVIISRALELFLESLLKKACQVTQSRNAKTMTTSHLKQCIELEQQFDFLKDLVASVPDMQGDGEDNHMDGDKGARRGRKPGSGGRKNGGMGTKSKDKKLSGTDSEQEDESEDTDTDGEEETSQPPPQASHPSAHFQSPPTPFLPFASTLPLPPAPPGPSAPDEEDEEDYDS

Target ORF information:

RefSeq Version	NM_006442.3
Organism	Homo sapiens(human)
Definition	Homo sapiens DR1 associated protein 1 (DRAP1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006442.3

ORF Insert Sequence:

ATGCCGAGCA AGAAGAAGAA GTACAACGCG CGGTTCCCGC CGGCGCGGAT CAAGAAGATC 
ATGCAGACGG ACGAAGAGAT TGGGAAGGTG GCGGCGGCGG TGCCTGTCAT CATCTCCCGG 
GCGCTCGAGC TCTTCCTAGA GTCGCTGTTG AAGAAGGCCT GCCAGGTGAC CCAGTCGCGG 
AACGCGAAGA CCATGACCAC ATCCCACCTG AAGCAGTGCA TCGAGCTGGA GCAGCAGTTT 
GACTTCTTGA AGGACCTGGT GGCATCTGTT CCCGACATGC AGGGGGACGG GGAAGACAAC 
CACATGGATG GGGACAAGGG CGCCCGCAGG GGCCGGAAGC CAGGCAGCGG CGGCCGGAAG 
AACGGTGGGA TGGGAACGAA AAGCAAGGAC AAGAAGCTGT CCGGGACAGA CTCGGAGCAG 
GAGGATGAAT CTGAGGACAC AGATACTGAT GGGGAAGAGG AGACATCACA ACCCCCACCC 
CAGGCCAGCC ACCCCTCTGC CCACTTTCAG AGCCCCCCGA CACCCTTCCT GCCCTTCGCC 
TCTACTCTGC CTTTGCCCCC AGCGCCCCCG GGCCCCTCAG CACCTGATGA AGAGGACGAA 
GAAGATTACG ACTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DRAP1 ( NM_006442.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_006433.2 Homo sapiens DR1 associated protein 1 (DRAP1), mRNA.

Price & Availability↑

CloneID	OHu55638
Clone ID Related Accession (Same CDS sequence)	NM_006442.3 , NM_006442.4
Accession Version	NM_006442.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 618bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-05
Organism	Homo sapiens(human)
Product	dr1-associated corepressor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BG036132.1, BC010025.2 and BQ773919.1. On Nov 22, 2018 this sequence version replaced NM_006442.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163658.428529.1, SRR1163658.289450.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000312515.7/ ENSP00000307850.2 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCCGAGCA AGAAGAAGAA GTACAACGCG CGGTTCCCGC CGGCGCGGAT CAAGAAGATC 
ATGCAGACGG ACGAAGAGAT TGGGAAGGTG GCGGCGGCGG TGCCTGTCAT CATCTCCCGG 
GCGCTCGAGC TCTTCCTAGA GTCGCTGTTG AAGAAGGCCT GCCAGGTGAC CCAGTCGCGG 
AACGCGAAGA CCATGACCAC ATCCCACCTG AAGCAGTGCA TCGAGCTGGA GCAGCAGTTT 
GACTTCTTGA AGGACCTGGT GGCATCTGTT CCCGACATGC AGGGGGACGG GGAAGACAAC 
CACATGGATG GGGACAAGGG CGCCCGCAGG GGCCGGAAGC CAGGCAGCGG CGGCCGGAAG 
AACGGTGGGA TGGGAACGAA AAGCAAGGAC AAGAAGCTGT CCGGGACAGA CTCGGAGCAG 
GAGGATGAAT CTGAGGACAC AGATACTGAT GGGGAAGAGG AGACATCACA ACCCCCACCC 
CAGGCCAGCC ACCCCTCTGC CCACTTTCAG AGCCCCCCGA CACCCTTCCT GCCCTTCGCC 
TCTACTCTGC CTTTGCCCCC AGCGCCCCCG GGCCCCTCAG CACCTGATGA AGAGGACGAA 
GAAGATTACG ACTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_006433.2
CDS	77..694
Translation	MPSKKKKYNARFPPARIKKIMQTDEEIGKVAAAVPVIISRALELFLESLLKKACQVTQSRNAKTMTTSHLKQCIELEQQFDFLKDLVASVPDMQGDGEDNHMDGDKGARRGRKPGSGGRKNGGMGTKSKDKKLSGTDSEQEDESEDTDTDGEEETSQPPPQASHPSAHFQSPPTPFLPFASTLPLPPAPPGPSAPDEEDEEDYDS

Target ORF information:

RefSeq Version	NM_006442.4
Organism	Homo sapiens(human)
Definition	Homo sapiens DR1 associated protein 1 (DRAP1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006442.4

ORF Insert Sequence:

ATGCCGAGCA AGAAGAAGAA GTACAACGCG CGGTTCCCGC CGGCGCGGAT CAAGAAGATC 
ATGCAGACGG ACGAAGAGAT TGGGAAGGTG GCGGCGGCGG TGCCTGTCAT CATCTCCCGG 
GCGCTCGAGC TCTTCCTAGA GTCGCTGTTG AAGAAGGCCT GCCAGGTGAC CCAGTCGCGG 
AACGCGAAGA CCATGACCAC ATCCCACCTG AAGCAGTGCA TCGAGCTGGA GCAGCAGTTT 
GACTTCTTGA AGGACCTGGT GGCATCTGTT CCCGACATGC AGGGGGACGG GGAAGACAAC 
CACATGGATG GGGACAAGGG CGCCCGCAGG GGCCGGAAGC CAGGCAGCGG CGGCCGGAAG 
AACGGTGGGA TGGGAACGAA AAGCAAGGAC AAGAAGCTGT CCGGGACAGA CTCGGAGCAG 
GAGGATGAAT CTGAGGACAC AGATACTGAT GGGGAAGAGG AGACATCACA ACCCCCACCC 
CAGGCCAGCC ACCCCTCTGC CCACTTTCAG AGCCCCCCGA CACCCTTCCT GCCCTTCGCC 
TCTACTCTGC CTTTGCCCCC AGCGCCCCCG GGCCCCTCAG CACCTGATGA AGAGGACGAA 
GAAGATTACG ACTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Pooled-matrix protein interaction screens using Barcode Fusion Genetics.
Molecular systems biology12(4)863(2016 Apr)
Yachie N,Petsalaki E,Mellor JC,Weile J,Jacob Y,Verby M,Ozturk SB,Li S,Cote AG,Mosca R,Knapp JJ,Ko M,Yu A,Gebbia M,Sahni N,Yi S,Tyagi T,Sheykhkarimli D,Roth JF,Wong C,Musa L,Snider J,Liu YC,Yu H,Braun P,Stagljar I,Hao T,Calderwood MA,Pelletier L,Aloy P,Hill DE,Vidal M,Roth FP

Genome-wide association study identifies three novel susceptibility loci for severe Acne vulgaris.
Nature communications54020(2014 Jun)
Navarini AA,Simpson MA,Weale M,Knight J,Carlavan I,Reiniche P,Burden DA,Layton A,Bataille V,Allen M,Pleass R,Pink A,Creamer D,English J,Munn S,Walton S,,Willis C,D?ret S,Voegel JJ,Spector T,Smith CH,Trembath RC,Barker JN

Next-generation sequencing to generate interactome datasets.
Nature methods8(6)478-80(2011 Jun)
Yu H,Tardivo L,Tam S,Weiner E,Gebreab F,Fan C,Svrzikapa N,Hirozane-Kishikawa T,Rietman E,Yang X,Sahalie J,Salehi-Ashtiani K,Hao T,Cusick ME,Hill DE,Roth FP,Braun P,Vidal M

A census of human transcription factors: function, expression and evolution.
Nature reviews. Genetics10(4)252-63(2009 04)
Vaquerizas JM,Kummerfeld SK,Teichmann SA,Luscombe NM

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DRAP1 cDNA ORF clone, Homo sapiens(human)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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