DCTN2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	DCTN2
Entrez Gene ID	10540
Full Name	dynactin subunit 2
Synonyms	DCTN50,DYNAMITIN,HEL-S-77,RBP50
General protein information	Preferred Names dynactin subunit 2 Names dynactin subunit 2 50 kDa dynein-associated polypeptide dynactin 2 (p50) dynactin complex 50 kDa subunit epididymis secretory protein Li 77 p50 dynamitin
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 12 Map Location: 12q13.3
Summary	This gene encodes a 50-kD subunit of dynactin, a macromolecular complex consisting of 10-11 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. It is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit is present in 4-5 copies per dynactin molecule. It contains three short alpha-helical coiled-coil domains that may mediate association with self or other dynactin subunits. It may interact directly with the largest subunit (p150) of dynactin and may affix p150 in place. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, May 2012].
Disorder MIM:	607376

mRNA and Protein(s)

mRNA	Protein	Name
NM_001348067.1	NP_001334996.1	dynactin subunit 2 isoform 6
NM_001261413.1	NP_001248342.1	dynactin subunit 2 isoform 3
NM_006400.4	NP_006391.1	dynactin subunit 2 isoform 1
NM_001348068.1	NP_001334997.1	dynactin subunit 2 isoform 6
NM_001348066.1	NP_001334995.1	dynactin subunit 2 isoform 5
NM_001348065.1	NP_001334994.1	dynactin subunit 2 isoform 4
NM_001261412.1	NP_001248341.1	dynactin subunit 2 isoform 2

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	DCTN2	NP_990133.1
	DCTN2	XP_001116130.1
Xenopus tropicalis (tropical clawed frog)	dctn2	NP_001015706.1
Rattus norvegicus (Norway rat)	Dctn2	NP_001004239.1
Danio rerio (zebrafish)	dctn2	NP_957460.1
Canis lupus familiaris (dog)	DCTN2	XP_005625602.1
Bos taurus (cattle)	DCTN2	NP_001029730.1
Mus musculus (house mouse)	Dctn2	NP_001177383.1
Drosophila melanogaster (fruit fly)	Dmn	NP_524690.1
Anopheles gambiae (African malaria mosquito)	DCTN2_ANOGA	XP_320820.3
Caenorhabditis elegans (roundworm)	dnc-2	NP_498286.1
Homo sapiens (human)	DCTN2	NP_001248341.1
Pan troglodytes (chimpanzee)	DCTN2	XP_003952191.1

Gene Ontology
Bibliography

Related articles in PubMed

Variants in the genes DCTN2, DNAH10, LRIG3, and MYO1A are associated with intermediate Charcot-Marie-Tooth disease in a Norwegian family.
Braathen GJ, H?yer H, Busk ?L, Tveten K, Skjelbred CF, Russell MB
Acta neurologica Scandinavica134(1)67-75(2016 Jul)

An organelle-specific protein landscape identifies novel diseases and molecular mechanisms.
Boldt K, van Reeuwijk J, Lu Q, Koutroumpas K, Nguyen TM, Texier Y, van Beersum SE, Horn N, Willer JR, Mans DA, Dougherty G, Lamers IJ, Coene KL, Arts HH, Betts MJ, Beyer T, Bolat E, Gloeckner CJ, Haidari K, Hetterschijt L, Iaconis D, Jenkins D, Klose F, Knapp B, Latour B, Letteboer SJ, Marcelis CL, Mitic D, Morleo M, Oud MM, Riemersma M, Rix S, Terhal PA, Toedt G, van Dam TJ, de Vrieze E, Wissinger Y, Wu KM, Apic G, Beales PL, Blacque OE, Gibson TJ, Huynen MA, Katsanis N, Kremer H, Omran H, van Wijk E, Wolfrum U, Kepes F, Davis EE, Franco B, Giles RH, Ueffing M, Russell RB, Roepman R,
Nature communications711491(2016 May)

A molecular mechanism to regulate lysosome motility for lysosome positioning and tubulation.
Li X, Rydzewski N, Hider A, Zhang X, Yang J, Wang W, Gao Q, Cheng X, Xu H
Nature cell biology18(4)404-17(2016 Apr)

Pooled-matrix protein interaction screens using Barcode Fusion Genetics.
Yachie N, Petsalaki E, Mellor JC, Weile J, Jacob Y, Verby M, Ozturk SB, Li S, Cote AG, Mosca R, Knapp JJ, Ko M, Yu A, Gebbia M, Sahni N, Yi S, Tyagi T, Sheykhkarimli D, Roth JF, Wong C, Musa L, Snider J, Liu YC, Yu H, Braun P, Stagljar I, Hao T, Calderwood MA, Pelletier L, Aloy P, Hill DE, Vidal M, Roth FP
Molecular systems biology12(4)863(2016 Apr)

A Dynamic Protein Interaction Landscape of the Human Centrosome-Cilium Interface.
Gupta GD, Coyaud ?, Gon?alves J, Mojarad BA, Liu Y, Wu Q, Gheiratmand L, Comartin D, Tkach JM, Cheung SW, Bashkurov M, Hasegan M, Knight JD, Lin ZY, Schueler M, Hildebrandt F, Moffat J, Gingras AC, Raught B, Pelletier L
Cell163(6)1484-99(2015 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This is the first time a haplotype on chromosome 12 containing sequence variants in the genes DCTN2, DNAH10, LRIG3, and MYO1A has been linked to an inherited neuropathy in humans.
Title: Variants in the genes DCTN2, DNAH10, LRIG3, and MYO1A are associated with intermediate Charcot-Marie-Tooth disease in a Norwegian family.

dynamitin is involved in the regulation of Nav1.5 cell-surface density
Title: Dynamitin affects cell-surface expression of voltage-gated sodium channel Nav1.5.

the determinants of p50/DM required for self-oligomerization of the protein and for interactions with other subunits of the dynactin complex
Title: Molecular and functional basis for the scaffolding role of the p50/dynamitin subunit of the microtubule-associated dynactin complex.

The interaction between ZW10 and dynamitin showed that the N-terminal region of ZW10 is the major binding site for dynamitin and, like full-length ZW10, could move along microtubules to the centrosomal area in a dynein-dynactin-dependent manner.
Title: N-terminal region of ZW10 serves not only as a determinant for localization but also as a link with dynein function.

Data show how dynamitin's different structural domains contribute to its ability to self-associate, interact with dynactin and assemble into a complex with its close binding partner, p24.
Title: Dynamitin mutagenesis reveals protein-protein interactions important for dynactin structure.

The following DCTN2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DCTN2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu09525	NM_001261412.1 Latest version!	Homo sapiens dynactin subunit 2 (DCTN2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu09733	NM_006400.4 Latest version!	Homo sapiens dynactin subunit 2 (DCTN2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu10144	NM_001261413.1 Latest version!	Homo sapiens dynactin subunit 2 (DCTN2), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu64112	NM_001348067.1 Latest version!	Homo sapiens dynactin subunit 2 (DCTN2), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
OHu64112	NM_001348068.1 Latest version!	Homo sapiens dynactin subunit 2 (DCTN2), transcript variant 7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
OHu102177	NM_001348065.1 Latest version!	Homo sapiens dynactin subunit 2 (DCTN2), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu102293	NM_001348066.1 Latest version!	Homo sapiens dynactin subunit 2 (DCTN2), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DCTN2 ( NM_001261412.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001248341.1 Homo sapiens dynactin subunit 2 (DCTN2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu09525
Clone ID Related Accession (Same CDS sequence)	NM_001261412.1
Accession Version	NM_001261412.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1212bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Homo sapiens(human)
Product	dynactin subunit 2 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB027733.1, AK098794.1 and DB569305.1. Transcript Variant: This variant (2) lacks an in-frame exon in the 5' coding region, compared to variant 1. The resulting isoform (2) lacks an internal segment, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.250102.1, SRR1660805.122319.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGACC CTAAATACGC CGACCTTCCC GGCATTGCCA GGAATGAGCC AGATGTTTAT 
GAAACTAGCG ACCTACCTGA GGATGATCAA GCGGAGTTCG ATGCGGAGCT GGAGGAGCTG 
ACAAGCACAA GTGTGGAACA CATCATTGTC AATCCTAATG CTGCCTATGA CAAGTTCAAG 
GACAAGAGAG TGGGGACAAA GGGACTTGAT TTCTCAGATC GTATTGGAAA AACCAAGAGG 
ACAGGATATG AATCTGGAGA ATATGAGATG CTTGGAGAGG GTCTGGGAGT GAAGGAGACA 
CCCCAGCAAA AGTACCAGCG CCTACTGCAT GAGGTCCAAG AGCTGACAAC TGAAGTTGAA 
AAAATCAAGA CGACAGTGAA GGAGTCAGCC ACAGAGGAGA AGCTGACCCC TGTGTTGCTG 
GCTAAACAGC TGGCAGCCCT GAAGCAGCAG CTGGTTGCTT CCCACCTGGA GAAGCTGCTG 
GGACCAGATG CTGCAATCAA CCTTACCGAC CCCGATGGCG CCCTGGCTAA GCGCCTACTA 
CTGCAGCTGG AAGCAACAAA GAACAGCAAA GGGGGATCAG GGGGAAAAAC CACTGGGACC 
CCCCCAGATA GCAGCCTTGT CACTTATGAA CTACATTCTC GGCCTGAGCA GGACAAGTTC 
TCTCAAGCTG CCAAAGTCGC AGAACTTGAA AAGCGCCTGA CAGAGCTGGA GACAGCTGTA 
CGTTGTGATC AGGATGCTCA GAATCCCCTT TCTGCAGGTC TACAGGGAGC CTGTCTCATG 
GAGACTGTAG AGCTGTTGCA AGCAAAGGTG AGCGCCCTAG ACCTTGCAGT TTTGGATCAA 
GTGGAGGCTC GGCTACAGAG TGTCCTGGGA AAGGTGAACG AGATTGCCAA GCATAAAGCC 
TCTGTAGAAG ATGCAGATAC ACAAAGCAAG GTGCACCAGC TATATGAAAC TATACAGCGC 
TGGAGCCCCA TTGCCTCCAC CCTCCCTGAG CTGGTGCAGA GACTTGTCAC CATCAAGCAG 
CTGCACGAGC AAGCCATGCA GTTTGGTCAG CTCCTGACAC ACTTGGATAC CACCCAGCAG 
ATGATTGCTA ATTCCTTGAA GGACAATACC ACCCTCTTGA CCCAGGTGCA GACAACCATG 
CGTGAAAACC TGGCCACAGT TGAGGGGAAC TTTGCCAGCA TTGATGAACG GATGAAGAAG 
CTGGGAAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001248341.1
CDS	269..1480
Translation	MADPKYADLPGIARNEPDVYETSDLPEDDQAEFDAELEELTSTSVEHIIVNPNAAYDKFKDKRVGTKGLDFSDRIGKTKRTGYESGEYEMLGEGLGVKETPQQKYQRLLHEVQELTTEVEKIKTTVKESATEEKLTPVLLAKQLAALKQQLVASHLEKLLGPDAAINLTDPDGALAKRLLLQLEATKNSKGGSGGKTTGTPPDSSLVTYELHSRPEQDKFSQAAKVAELEKRLTELETAVRCDQDAQNPLSAGLQGACLMETVELLQAKVSALDLAVLDQVEARLQSVLGKVNEIAKHKASVEDADTQSKVHQLYETIQRWSPIASTLPELVQRLVTIKQLHEQAMQFGQLLTHLDTTQQMIANSLKDNTTLLTQVQTTMRENLATVEGNFASIDERMKKLGK

Target ORF information:

RefSeq Version	NM_001261412.1
Organism	Homo sapiens(human)
Definition	Homo sapiens dynactin subunit 2 (DCTN2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001261412.1

ORF Insert Sequence:

ATGGCGGACC CTAAATACGC CGACCTTCCC GGCATTGCCA GGAATGAGCC AGATGTTTAT 
GAAACTAGCG ACCTACCTGA GGATGATCAA GCGGAGTTCG ATGCGGAGCT GGAGGAGCTG 
ACAAGCACAA GTGTGGAACA CATCATTGTC AATCCTAATG CTGCCTATGA CAAGTTCAAG 
GACAAGAGAG TGGGGACAAA GGGACTTGAT TTCTCAGATC GTATTGGAAA AACCAAGAGG 
ACAGGATATG AATCTGGAGA ATATGAGATG CTTGGAGAGG GTCTGGGAGT GAAGGAGACA 
CCCCAGCAAA AGTACCAGCG CCTACTGCAT GAGGTCCAAG AGCTGACAAC TGAAGTTGAA 
AAAATCAAGA CGACAGTGAA GGAGTCAGCC ACAGAGGAGA AGCTGACCCC TGTGTTGCTG 
GCTAAACAGC TGGCAGCCCT GAAGCAGCAG CTGGTTGCTT CCCACCTGGA GAAGCTGCTG 
GGACCAGATG CTGCAATCAA CCTTACCGAC CCCGATGGCG CCCTGGCTAA GCGCCTACTA 
CTGCAGCTGG AAGCAACAAA GAACAGCAAA GGGGGATCAG GGGGAAAAAC CACTGGGACC 
CCCCCAGATA GCAGCCTTGT CACTTATGAA CTACATTCTC GGCCTGAGCA GGACAAGTTC 
TCTCAAGCTG CCAAAGTCGC AGAACTTGAA AAGCGCCTGA CAGAGCTGGA GACAGCTGTA 
CGTTGTGATC AGGATGCTCA GAATCCCCTT TCTGCAGGTC TACAGGGAGC CTGTCTCATG 
GAGACTGTAG AGCTGTTGCA AGCAAAGGTG AGCGCCCTAG ACCTTGCAGT TTTGGATCAA 
GTGGAGGCTC GGCTACAGAG TGTCCTGGGA AAGGTGAACG AGATTGCCAA GCATAAAGCC 
TCTGTAGAAG ATGCAGATAC ACAAAGCAAG GTGCACCAGC TATATGAAAC TATACAGCGC 
TGGAGCCCCA TTGCCTCCAC CCTCCCTGAG CTGGTGCAGA GACTTGTCAC CATCAAGCAG 
CTGCACGAGC AAGCCATGCA GTTTGGTCAG CTCCTGACAC ACTTGGATAC CACCCAGCAG 
ATGATTGCTA ATTCCTTGAA GGACAATACC ACCCTCTTGA CCCAGGTGCA GACAACCATG 
CGTGAAAACC TGGCCACAGT TGAGGGGAAC TTTGCCAGCA TTGATGAACG GATGAAGAAG 
CTGGGAAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DCTN2 ( NM_006400.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_006391.1 Homo sapiens dynactin subunit 2 (DCTN2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu09733
Clone ID Related Accession (Same CDS sequence)	NM_006400.4
Accession Version	NM_006400.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1221bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Homo sapiens(human)
Product	dynactin subunit 2 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC022506.38. This sequence is a reference standard in the RefSeqGene project. On May 12, 2012 this sequence version replaced NM_006400.3. Transcript Variant: This variant (1) encodes isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.5626.1, SRR1660805.151197.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGACC CTAAATACGC CGACCTTCCC GGCATTGCCA GGAATGAGCC AGATGTTTAT 
GAAACTAGCG ACCTACCTGA GGATGATCAA GCGGAGTTCG ATGCGTTTGC ACAAGAGCTG 
GAGGAGCTGA CAAGCACAAG TGTGGAACAC ATCATTGTCA ATCCTAATGC TGCCTATGAC 
AAGTTCAAGG ACAAGAGAGT GGGGACAAAG GGACTTGATT TCTCAGATCG TATTGGAAAA 
ACCAAGAGGA CAGGATATGA ATCTGGAGAA TATGAGATGC TTGGAGAGGG TCTGGGAGTG 
AAGGAGACAC CCCAGCAAAA GTACCAGCGC CTACTGCATG AGGTCCAAGA GCTGACAACT 
GAAGTTGAAA AAATCAAGAC GACAGTGAAG GAGTCAGCCA CAGAGGAGAA GCTGACCCCT 
GTGTTGCTGG CTAAACAGCT GGCAGCCCTG AAGCAGCAGC TGGTTGCTTC CCACCTGGAG 
AAGCTGCTGG GACCAGATGC TGCAATCAAC CTTACCGACC CCGATGGCGC CCTGGCTAAG 
CGCCTACTAC TGCAGCTGGA AGCAACAAAG AACAGCAAAG GGGGATCAGG GGGAAAAACC 
ACTGGGACCC CCCCAGATAG CAGCCTTGTC ACTTATGAAC TACATTCTCG GCCTGAGCAG 
GACAAGTTCT CTCAAGCTGC CAAAGTCGCA GAACTTGAAA AGCGCCTGAC AGAGCTGGAG 
ACAGCTGTAC GTTGTGATCA GGATGCTCAG AATCCCCTTT CTGCAGGTCT ACAGGGAGCC 
TGTCTCATGG AGACTGTAGA GCTGTTGCAA GCAAAGGTGA GCGCCCTAGA CCTTGCAGTT 
TTGGATCAAG TGGAGGCTCG GCTACAGAGT GTCCTGGGAA AGGTGAACGA GATTGCCAAG 
CATAAAGCCT CTGTAGAAGA TGCAGATACA CAAAGCAAGG TGCACCAGCT ATATGAAACT 
ATACAGCGCT GGAGCCCCAT TGCCTCCACC CTCCCTGAGC TGGTGCAGAG ACTTGTCACC 
ATCAAGCAGC TGCACGAGCA AGCCATGCAG TTTGGTCAGC TCCTGACACA CTTGGATACC 
ACCCAGCAGA TGATTGCTAA TTCCTTGAAG GACAATACCA CCCTCTTGAC CCAGGTGCAG 
ACAACCATGC GTGAAAACCT GGCCACAGTT GAGGGGAACT TTGCCAGCAT TGATGAACGG 
ATGAAGAAGC TGGGAAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_006391.1
CDS	269..1489
Translation	MADPKYADLPGIARNEPDVYETSDLPEDDQAEFDAFAQELEELTSTSVEHIIVNPNAAYDKFKDKRVGTKGLDFSDRIGKTKRTGYESGEYEMLGEGLGVKETPQQKYQRLLHEVQELTTEVEKIKTTVKESATEEKLTPVLLAKQLAALKQQLVASHLEKLLGPDAAINLTDPDGALAKRLLLQLEATKNSKGGSGGKTTGTPPDSSLVTYELHSRPEQDKFSQAAKVAELEKRLTELETAVRCDQDAQNPLSAGLQGACLMETVELLQAKVSALDLAVLDQVEARLQSVLGKVNEIAKHKASVEDADTQSKVHQLYETIQRWSPIASTLPELVQRLVTIKQLHEQAMQFGQLLTHLDTTQQMIANSLKDNTTLLTQVQTTMRENLATVEGNFASIDERMKKLGK

Target ORF information:

RefSeq Version	NM_006400.4
Organism	Homo sapiens(human)
Definition	Homo sapiens dynactin subunit 2 (DCTN2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006400.4

ORF Insert Sequence:

ATGGCGGACC CTAAATACGC CGACCTTCCC GGCATTGCCA GGAATGAGCC AGATGTTTAT 
GAAACTAGCG ACCTACCTGA GGATGATCAA GCGGAGTTCG ATGCGTTTGC ACAAGAGCTG 
GAGGAGCTGA CAAGCACAAG TGTGGAACAC ATCATTGTCA ATCCTAATGC TGCCTATGAC 
AAGTTCAAGG ACAAGAGAGT GGGGACAAAG GGACTTGATT TCTCAGATCG TATTGGAAAA 
ACCAAGAGGA CAGGATATGA ATCTGGAGAA TATGAGATGC TTGGAGAGGG TCTGGGAGTG 
AAGGAGACAC CCCAGCAAAA GTACCAGCGC CTACTGCATG AGGTCCAAGA GCTGACAACT 
GAAGTTGAAA AAATCAAGAC GACAGTGAAG GAGTCAGCCA CAGAGGAGAA GCTGACCCCT 
GTGTTGCTGG CTAAACAGCT GGCAGCCCTG AAGCAGCAGC TGGTTGCTTC CCACCTGGAG 
AAGCTGCTGG GACCAGATGC TGCAATCAAC CTTACCGACC CCGATGGCGC CCTGGCTAAG 
CGCCTACTAC TGCAGCTGGA AGCAACAAAG AACAGCAAAG GGGGATCAGG GGGAAAAACC 
ACTGGGACCC CCCCAGATAG CAGCCTTGTC ACTTATGAAC TACATTCTCG GCCTGAGCAG 
GACAAGTTCT CTCAAGCTGC CAAAGTCGCA GAACTTGAAA AGCGCCTGAC AGAGCTGGAG 
ACAGCTGTAC GTTGTGATCA GGATGCTCAG AATCCCCTTT CTGCAGGTCT ACAGGGAGCC 
TGTCTCATGG AGACTGTAGA GCTGTTGCAA GCAAAGGTGA GCGCCCTAGA CCTTGCAGTT 
TTGGATCAAG TGGAGGCTCG GCTACAGAGT GTCCTGGGAA AGGTGAACGA GATTGCCAAG 
CATAAAGCCT CTGTAGAAGA TGCAGATACA CAAAGCAAGG TGCACCAGCT ATATGAAACT 
ATACAGCGCT GGAGCCCCAT TGCCTCCACC CTCCCTGAGC TGGTGCAGAG ACTTGTCACC 
ATCAAGCAGC TGCACGAGCA AGCCATGCAG TTTGGTCAGC TCCTGACACA CTTGGATACC 
ACCCAGCAGA TGATTGCTAA TTCCTTGAAG GACAATACCA CCCTCTTGAC CCAGGTGCAG 
ACAACCATGC GTGAAAACCT GGCCACAGTT GAGGGGAACT TTGCCAGCAT TGATGAACGG 
ATGAAGAAGC TGGGAAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DCTN2 ( NM_001261413.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001248342.1 Homo sapiens dynactin subunit 2 (DCTN2), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu10144
Clone ID Related Accession (Same CDS sequence)	NM_001261413.1
Accession Version	NM_001261413.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1206bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Homo sapiens(human)
Product	dynactin subunit 2 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB027733.1, BC009468.1 and DB569305.1. Transcript Variant: This variant (3) lacks two exons in the 5' coding region but maintains the reading frame, compared to variant 1. The resulting isoform (3) lacks an internal segment, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.174070.1, SRR1163657.468951.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGGACC CTAAATACGC CGACCTTCCC GGCATTGCCA GGAATGAGCC AGATGTTTAT 
GAAACTAGCG ACCTACCTGA GGATGATCAA GCGGAGTTCG ATGCGGAGGA GCTGACAAGC 
ACAAGTGTGG AACACATCAT TGTCAATCCT AATGCTGCCT ATGACAAGTT CAAGGACAAG 
AGAGTGGGGA CAAAGGGACT TGATTTCTCA GATCGTATTG GAAAAACCAA GAGGACAGGA 
TATGAATCTG GAGAATATGA GATGCTTGGA GAGGGTCTGG GAGTGAAGGA GACACCCCAG 
CAAAAGTACC AGCGCCTACT GCATGAGGTC CAAGAGCTGA CAACTGAAGT TGAAAAAATC 
AAGACGACAG TGAAGGAGTC AGCCACAGAG GAGAAGCTGA CCCCTGTGTT GCTGGCTAAA 
CAGCTGGCAG CCCTGAAGCA GCAGCTGGTT GCTTCCCACC TGGAGAAGCT GCTGGGACCA 
GATGCTGCAA TCAACCTTAC CGACCCCGAT GGCGCCCTGG CTAAGCGCCT ACTACTGCAG 
CTGGAAGCAA CAAAGAACAG CAAAGGGGGA TCAGGGGGAA AAACCACTGG GACCCCCCCA 
GATAGCAGCC TTGTCACTTA TGAACTACAT TCTCGGCCTG AGCAGGACAA GTTCTCTCAA 
GCTGCCAAAG TCGCAGAACT TGAAAAGCGC CTGACAGAGC TGGAGACAGC TGTACGTTGT 
GATCAGGATG CTCAGAATCC CCTTTCTGCA GGTCTACAGG GAGCCTGTCT CATGGAGACT 
GTAGAGCTGT TGCAAGCAAA GGTGAGCGCC CTAGACCTTG CAGTTTTGGA TCAAGTGGAG 
GCTCGGCTAC AGAGTGTCCT GGGAAAGGTG AACGAGATTG CCAAGCATAA AGCCTCTGTA 
GAAGATGCAG ATACACAAAG CAAGGTGCAC CAGCTATATG AAACTATACA GCGCTGGAGC 
CCCATTGCCT CCACCCTCCC TGAGCTGGTG CAGAGACTTG TCACCATCAA GCAGCTGCAC 
GAGCAAGCCA TGCAGTTTGG TCAGCTCCTG ACACACTTGG ATACCACCCA GCAGATGATT 
GCTAATTCCT TGAAGGACAA TACCACCCTC TTGACCCAGG TGCAGACAAC CATGCGTGAA 
AACCTGGCCA CAGTTGAGGG GAACTTTGCC AGCATTGATG AACGGATGAA GAAGCTGGGA 
AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001248342.1
CDS	269..1474
Translation	MADPKYADLPGIARNEPDVYETSDLPEDDQAEFDAEELTSTSVEHIIVNPNAAYDKFKDKRVGTKGLDFSDRIGKTKRTGYESGEYEMLGEGLGVKETPQQKYQRLLHEVQELTTEVEKIKTTVKESATEEKLTPVLLAKQLAALKQQLVASHLEKLLGPDAAINLTDPDGALAKRLLLQLEATKNSKGGSGGKTTGTPPDSSLVTYELHSRPEQDKFSQAAKVAELEKRLTELETAVRCDQDAQNPLSAGLQGACLMETVELLQAKVSALDLAVLDQVEARLQSVLGKVNEIAKHKASVEDADTQSKVHQLYETIQRWSPIASTLPELVQRLVTIKQLHEQAMQFGQLLTHLDTTQQMIANSLKDNTTLLTQVQTTMRENLATVEGNFASIDERMKKLGK

Target ORF information:

RefSeq Version	NM_001261413.1
Organism	Homo sapiens(human)
Definition	Homo sapiens dynactin subunit 2 (DCTN2), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001261413.1

ORF Insert Sequence:

ATGGCGGACC CTAAATACGC CGACCTTCCC GGCATTGCCA GGAATGAGCC AGATGTTTAT 
GAAACTAGCG ACCTACCTGA GGATGATCAA GCGGAGTTCG ATGCGGAGGA GCTGACAAGC 
ACAAGTGTGG AACACATCAT TGTCAATCCT AATGCTGCCT ATGACAAGTT CAAGGACAAG 
AGAGTGGGGA CAAAGGGACT TGATTTCTCA GATCGTATTG GAAAAACCAA GAGGACAGGA 
TATGAATCTG GAGAATATGA GATGCTTGGA GAGGGTCTGG GAGTGAAGGA GACACCCCAG 
CAAAAGTACC AGCGCCTACT GCATGAGGTC CAAGAGCTGA CAACTGAAGT TGAAAAAATC 
AAGACGACAG TGAAGGAGTC AGCCACAGAG GAGAAGCTGA CCCCTGTGTT GCTGGCTAAA 
CAGCTGGCAG CCCTGAAGCA GCAGCTGGTT GCTTCCCACC TGGAGAAGCT GCTGGGACCA 
GATGCTGCAA TCAACCTTAC CGACCCCGAT GGCGCCCTGG CTAAGCGCCT ACTACTGCAG 
CTGGAAGCAA CAAAGAACAG CAAAGGGGGA TCAGGGGGAA AAACCACTGG GACCCCCCCA 
GATAGCAGCC TTGTCACTTA TGAACTACAT TCTCGGCCTG AGCAGGACAA GTTCTCTCAA 
GCTGCCAAAG TCGCAGAACT TGAAAAGCGC CTGACAGAGC TGGAGACAGC TGTACGTTGT 
GATCAGGATG CTCAGAATCC CCTTTCTGCA GGTCTACAGG GAGCCTGTCT CATGGAGACT 
GTAGAGCTGT TGCAAGCAAA GGTGAGCGCC CTAGACCTTG CAGTTTTGGA TCAAGTGGAG 
GCTCGGCTAC AGAGTGTCCT GGGAAAGGTG AACGAGATTG CCAAGCATAA AGCCTCTGTA 
GAAGATGCAG ATACACAAAG CAAGGTGCAC CAGCTATATG AAACTATACA GCGCTGGAGC 
CCCATTGCCT CCACCCTCCC TGAGCTGGTG CAGAGACTTG TCACCATCAA GCAGCTGCAC 
GAGCAAGCCA TGCAGTTTGG TCAGCTCCTG ACACACTTGG ATACCACCCA GCAGATGATT 
GCTAATTCCT TGAAGGACAA TACCACCCTC TTGACCCAGG TGCAGACAAC CATGCGTGAA 
AACCTGGCCA CAGTTGAGGG GAACTTTGCC AGCATTGATG AACGGATGAA GAAGCTGGGA 
AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DCTN2 ( NM_001348067.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001334996.1 Homo sapiens dynactin subunit 2 (DCTN2), transcript variant 6, mRNA.

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CloneID	OHu64112
Clone ID Related Accession (Same CDS sequence)	NM_001348067.1 , NM_001348068.1
Accession Version	NM_001348067.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 945bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-05
Organism	Homo sapiens(human)
Product	dynactin subunit 2 isoform 6
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC022506.38 and AK292364.1. Transcript Variant: This variant (6), as well as variant 7, encodes isoform 6. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK292364.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTTGGAG AGGGTCTGGG AGTGAAGGAG ACACCCCAGC AAAAGTACCA GCGCCTACTG 
CATGAGGTCC AAGAGCTGAC AACTGAAGTT GAAAAAATCA AGACGACAGT GAAGGAGTCA 
GCCACAGAGG AGAAGCTGAC CCCTGTGTTG CTGGCTAAAC AGCTGGCAGC CCTGAAGCAG 
CAGCTGGTTG CTTCCCACCT GGAGAAGCTG CTGGGACCAG ATGCTGCAAT CAACCTTACC 
GACCCCGATG GCGCCCTGGC TAAGCGCCTA CTACTGCAGC TGGAAGCAAC AAAGAACAGC 
AAAGGGGGAT CAGGGGGAAA AACCACTGGG ACCCCCCCAG ATAGCAGCCT TGTCACTTAT 
GAACTACATT CTCGGCCTGA GCAGGACAAG TTCTCTCAAG CTGCCAAAGT CGCAGAACTT 
GAAAAGCGCC TGACAGAGCT GGAGACAGCT GTACGTTGTG ATCAGGATGC TCAGAATCCC 
CTTTCTGCAG GTCTACAGGG AGCCTGTCTC ATGGAGACTG TAGAGCTGTT GCAAGCAAAG 
GTGAGCGCCC TAGACCTTGC AGTTTTGGAT CAAGTGGAGG CTCGGCTACA GAGTGTCCTG 
GGAAAGGTGA ACGAGATTGC CAAGCATAAA GCCTCTGTAG AAGATGCAGA TACACAAAGC 
AAGGTGCACC AGCTATATGA AACTATACAG CGCTGGAGCC CCATTGCCTC CACCCTCCCT 
GAGCTGGTGC AGAGACTTGT CACCATCAAG CAGCTGCACG AGCAAGCCAT GCAGTTTGGT 
CAGCTCCTGA CACACTTGGA TACCACCCAG CAGATGATTG CTAATTCCTT GAAGGACAAT 
ACCACCCTCT TGACCCAGGT GCAGACAACC ATGCGTGAAA ACCTGGCCAC AGTTGAGGGG 
AACTTTGCCA GCATTGATGA ACGGATGAAG AAGCTGGGAA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001334996.1
CDS	634..1578
Translation	MLGEGLGVKETPQQKYQRLLHEVQELTTEVEKIKTTVKESATEEKLTPVLLAKQLAALKQQLVASHLEKLLGPDAAINLTDPDGALAKRLLLQLEATKNSKGGSGGKTTGTPPDSSLVTYELHSRPEQDKFSQAAKVAELEKRLTELETAVRCDQDAQNPLSAGLQGACLMETVELLQAKVSALDLAVLDQVEARLQSVLGKVNEIAKHKASVEDADTQSKVHQLYETIQRWSPIASTLPELVQRLVTIKQLHEQAMQFGQLLTHLDTTQQMIANSLKDNTTLLTQVQTTMRENLATVEGNFASIDERMKKLGK

Target ORF information:

RefSeq Version	NM_001348067.1
Organism	Homo sapiens(human)
Definition	Homo sapiens dynactin subunit 2 (DCTN2), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001348067.1

ORF Insert Sequence:

ATGCTTGGAG AGGGTCTGGG AGTGAAGGAG ACACCCCAGC AAAAGTACCA GCGCCTACTG 
CATGAGGTCC AAGAGCTGAC AACTGAAGTT GAAAAAATCA AGACGACAGT GAAGGAGTCA 
GCCACAGAGG AGAAGCTGAC CCCTGTGTTG CTGGCTAAAC AGCTGGCAGC CCTGAAGCAG 
CAGCTGGTTG CTTCCCACCT GGAGAAGCTG CTGGGACCAG ATGCTGCAAT CAACCTTACC 
GACCCCGATG GCGCCCTGGC TAAGCGCCTA CTACTGCAGC TGGAAGCAAC AAAGAACAGC 
AAAGGGGGAT CAGGGGGAAA AACCACTGGG ACCCCCCCAG ATAGCAGCCT TGTCACTTAT 
GAACTACATT CTCGGCCTGA GCAGGACAAG TTCTCTCAAG CTGCCAAAGT CGCAGAACTT 
GAAAAGCGCC TGACAGAGCT GGAGACAGCT GTACGTTGTG ATCAGGATGC TCAGAATCCC 
CTTTCTGCAG GTCTACAGGG AGCCTGTCTC ATGGAGACTG TAGAGCTGTT GCAAGCAAAG 
GTGAGCGCCC TAGACCTTGC AGTTTTGGAT CAAGTGGAGG CTCGGCTACA GAGTGTCCTG 
GGAAAGGTGA ACGAGATTGC CAAGCATAAA GCCTCTGTAG AAGATGCAGA TACACAAAGC 
AAGGTGCACC AGCTATATGA AACTATACAG CGCTGGAGCC CCATTGCCTC CACCCTCCCT 
GAGCTGGTGC AGAGACTTGT CACCATCAAG CAGCTGCACG AGCAAGCCAT GCAGTTTGGT 
CAGCTCCTGA CACACTTGGA TACCACCCAG CAGATGATTG CTAATTCCTT GAAGGACAAT 
ACCACCCTCT TGACCCAGGT GCAGACAACC ATGCGTGAAA ACCTGGCCAC AGTTGAGGGG 
AACTTTGCCA GCATTGATGA ACGGATGAAG AAGCTGGGAA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DCTN2 ( NM_001348068.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001334997.1 Homo sapiens dynactin subunit 2 (DCTN2), transcript variant 7, mRNA.

Price & Availability↑

CloneID	OHu64112
Clone ID Related Accession (Same CDS sequence)	NM_001348067.1 , NM_001348068.1
Accession Version	NM_001348068.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 945bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-05
Organism	Homo sapiens(human)
Product	dynactin subunit 2 isoform 6
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC022506.38. On Jan 7, 2017 this sequence version replaced XM_011537761.2. Transcript Variant: This variant (7), as well as variant 6, encodes isoform 6. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: ERR279848.3868.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTTGGAG AGGGTCTGGG AGTGAAGGAG ACACCCCAGC AAAAGTACCA GCGCCTACTG 
CATGAGGTCC AAGAGCTGAC AACTGAAGTT GAAAAAATCA AGACGACAGT GAAGGAGTCA 
GCCACAGAGG AGAAGCTGAC CCCTGTGTTG CTGGCTAAAC AGCTGGCAGC CCTGAAGCAG 
CAGCTGGTTG CTTCCCACCT GGAGAAGCTG CTGGGACCAG ATGCTGCAAT CAACCTTACC 
GACCCCGATG GCGCCCTGGC TAAGCGCCTA CTACTGCAGC TGGAAGCAAC AAAGAACAGC 
AAAGGGGGAT CAGGGGGAAA AACCACTGGG ACCCCCCCAG ATAGCAGCCT TGTCACTTAT 
GAACTACATT CTCGGCCTGA GCAGGACAAG TTCTCTCAAG CTGCCAAAGT CGCAGAACTT 
GAAAAGCGCC TGACAGAGCT GGAGACAGCT GTACGTTGTG ATCAGGATGC TCAGAATCCC 
CTTTCTGCAG GTCTACAGGG AGCCTGTCTC ATGGAGACTG TAGAGCTGTT GCAAGCAAAG 
GTGAGCGCCC TAGACCTTGC AGTTTTGGAT CAAGTGGAGG CTCGGCTACA GAGTGTCCTG 
GGAAAGGTGA ACGAGATTGC CAAGCATAAA GCCTCTGTAG AAGATGCAGA TACACAAAGC 
AAGGTGCACC AGCTATATGA AACTATACAG CGCTGGAGCC CCATTGCCTC CACCCTCCCT 
GAGCTGGTGC AGAGACTTGT CACCATCAAG CAGCTGCACG AGCAAGCCAT GCAGTTTGGT 
CAGCTCCTGA CACACTTGGA TACCACCCAG CAGATGATTG CTAATTCCTT GAAGGACAAT 
ACCACCCTCT TGACCCAGGT GCAGACAACC ATGCGTGAAA ACCTGGCCAC AGTTGAGGGG 
AACTTTGCCA GCATTGATGA ACGGATGAAG AAGCTGGGAA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001334997.1
CDS	625..1569
Translation	MLGEGLGVKETPQQKYQRLLHEVQELTTEVEKIKTTVKESATEEKLTPVLLAKQLAALKQQLVASHLEKLLGPDAAINLTDPDGALAKRLLLQLEATKNSKGGSGGKTTGTPPDSSLVTYELHSRPEQDKFSQAAKVAELEKRLTELETAVRCDQDAQNPLSAGLQGACLMETVELLQAKVSALDLAVLDQVEARLQSVLGKVNEIAKHKASVEDADTQSKVHQLYETIQRWSPIASTLPELVQRLVTIKQLHEQAMQFGQLLTHLDTTQQMIANSLKDNTTLLTQVQTTMRENLATVEGNFASIDERMKKLGK

Target ORF information:

RefSeq Version	NM_001348068.1
Organism	Homo sapiens(human)
Definition	Homo sapiens dynactin subunit 2 (DCTN2), transcript variant 7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001348068.1

ORF Insert Sequence:

ATGCTTGGAG AGGGTCTGGG AGTGAAGGAG ACACCCCAGC AAAAGTACCA GCGCCTACTG 
CATGAGGTCC AAGAGCTGAC AACTGAAGTT GAAAAAATCA AGACGACAGT GAAGGAGTCA 
GCCACAGAGG AGAAGCTGAC CCCTGTGTTG CTGGCTAAAC AGCTGGCAGC CCTGAAGCAG 
CAGCTGGTTG CTTCCCACCT GGAGAAGCTG CTGGGACCAG ATGCTGCAAT CAACCTTACC 
GACCCCGATG GCGCCCTGGC TAAGCGCCTA CTACTGCAGC TGGAAGCAAC AAAGAACAGC 
AAAGGGGGAT CAGGGGGAAA AACCACTGGG ACCCCCCCAG ATAGCAGCCT TGTCACTTAT 
GAACTACATT CTCGGCCTGA GCAGGACAAG TTCTCTCAAG CTGCCAAAGT CGCAGAACTT 
GAAAAGCGCC TGACAGAGCT GGAGACAGCT GTACGTTGTG ATCAGGATGC TCAGAATCCC 
CTTTCTGCAG GTCTACAGGG AGCCTGTCTC ATGGAGACTG TAGAGCTGTT GCAAGCAAAG 
GTGAGCGCCC TAGACCTTGC AGTTTTGGAT CAAGTGGAGG CTCGGCTACA GAGTGTCCTG 
GGAAAGGTGA ACGAGATTGC CAAGCATAAA GCCTCTGTAG AAGATGCAGA TACACAAAGC 
AAGGTGCACC AGCTATATGA AACTATACAG CGCTGGAGCC CCATTGCCTC CACCCTCCCT 
GAGCTGGTGC AGAGACTTGT CACCATCAAG CAGCTGCACG AGCAAGCCAT GCAGTTTGGT 
CAGCTCCTGA CACACTTGGA TACCACCCAG CAGATGATTG CTAATTCCTT GAAGGACAAT 
ACCACCCTCT TGACCCAGGT GCAGACAACC ATGCGTGAAA ACCTGGCCAC AGTTGAGGGG 
AACTTTGCCA GCATTGATGA ACGGATGAAG AAGCTGGGAA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DCTN2 ( NM_001348065.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001334994.1 Homo sapiens dynactin subunit 2 (DCTN2), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu102177
Clone ID Related Accession (Same CDS sequence)	NM_001348065.1
Accession Version	NM_001348065.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1341bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-05
Organism	Homo sapiens(human)
Product	dynactin subunit 2 isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC022506.38. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.136477.1, SRR1660803.92494.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCTTCA TCCTGCCAAG GAAGAGGTTC TGCCATCTGG CAGAACCCTC TTGTGCCAGA 
TGGGGAGCTC TATCCTTGAG GGAACCTATG CCCTCCCCCA GCCCCCACTC CCATGACAAT 
CCTGGCGTGT ACCTTCCTTT TGTGCTGCCC TGCCTGCTTC TCAAGGCCAG GAATGAGCCA 
GATGTTTATG AAACTAGCGA CCTACCTGAG GATGATCAAG CGGAGTTCGA TGCGGAGCTG 
GAGGAGCTGA CAAGCACAAG TGTGGAACAC ATCATTGTCA ATCCTAATGC TGCCTATGAC 
AAGTTCAAGG ACAAGAGAGT GGGGACAAAG GGACTTGATT TCTCAGATCG TATTGGAAAA 
ACCAAGAGGA CAGGATATGA ATCTGGAGAA TATGAGATGC TTGGAGAGGG TCTGGGAGTG 
AAGGAGACAC CCCAGCAAAA GTACCAGCGC CTACTGCATG AGGTCCAAGA GCTGACAACT 
GAAGTTGAAA AAATCAAGAC GACAGTGAAG GAGTCAGCCA CAGAGGAGAA GCTGACCCCT 
GTGTTGCTGG CTAAACAGCT GGCAGCCCTG AAGCAGCAGC TGGTTGCTTC CCACCTGGAG 
AAGCTGCTGG GACCAGATGC TGCAATCAAC CTTACCGACC CCGATGGCGC CCTGGCTAAG 
CGCCTACTAC TGCAGCTGGA AGCAACAAAG AACAGCAAAG GGGGATCAGG GGGAAAAACC 
ACTGGGACCC CCCCAGATAG CAGCCTTGTC ACTTATGAAC TACATTCTCG GCCTGAGCAG 
GACAAGTTCT CTCAAGCTGC CAAAGTCGCA GAACTTGAAA AGCGCCTGAC AGAGCTGGAG 
ACAGCTGTAC GTTGTGATCA GGATGCTCAG AATCCCCTTT CTGCAGGTCT ACAGGGAGCC 
TGTCTCATGG AGACTGTAGA GCTGTTGCAA GCAAAGGTGA GCGCCCTAGA CCTTGCAGTT 
TTGGATCAAG TGGAGGCTCG GCTACAGAGT GTCCTGGGAA AGGTGAACGA GATTGCCAAG 
CATAAAGCCT CTGTAGAAGA TGCAGATACA CAAAGCAAGG TGCACCAGCT ATATGAAACT 
ATACAGCGCT GGAGCCCCAT TGCCTCCACC CTCCCTGAGC TGGTGCAGAG ACTTGTCACC 
ATCAAGCAGC TGCACGAGCA AGCCATGCAG TTTGGTCAGC TCCTGACACA CTTGGATACC 
ACCCAGCAGA TGATTGCTAA TTCCTTGAAG GACAATACCA CCCTCTTGAC CCAGGTGCAG 
ACAACCATGC GTGAAAACCT GGCCACAGTT GAGGGGAACT TTGCCAGCAT TGATGAACGG 
ATGAAGAAGC TGGGAAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001334994.1
CDS	200..1540
Translation	MAFILPRKRFCHLAEPSCARWGALSLREPMPSPSPHSHDNPGVYLPFVLPCLLLKARNEPDVYETSDLPEDDQAEFDAELEELTSTSVEHIIVNPNAAYDKFKDKRVGTKGLDFSDRIGKTKRTGYESGEYEMLGEGLGVKETPQQKYQRLLHEVQELTTEVEKIKTTVKESATEEKLTPVLLAKQLAALKQQLVASHLEKLLGPDAAINLTDPDGALAKRLLLQLEATKNSKGGSGGKTTGTPPDSSLVTYELHSRPEQDKFSQAAKVAELEKRLTELETAVRCDQDAQNPLSAGLQGACLMETVELLQAKVSALDLAVLDQVEARLQSVLGKVNEIAKHKASVEDADTQSKVHQLYETIQRWSPIASTLPELVQRLVTIKQLHEQAMQFGQLLTHLDTTQQMIANSLKDNTTLLTQVQTTMRENLATVEGNFASIDERMKKLGK

Target ORF information:

RefSeq Version	NM_001348065.1
Organism	Homo sapiens(human)
Definition	Homo sapiens dynactin subunit 2 (DCTN2), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001348065.1

ORF Insert Sequence:

ATGGCCTTCA TCCTGCCAAG GAAGAGGTTC TGCCATCTGG CAGAACCCTC TTGTGCCAGA 
TGGGGAGCTC TATCCTTGAG GGAACCTATG CCCTCCCCCA GCCCCCACTC CCATGACAAT 
CCTGGCGTGT ACCTTCCTTT TGTGCTGCCC TGCCTGCTTC TCAAGGCCAG GAATGAGCCA 
GATGTTTATG AAACTAGCGA CCTACCTGAG GATGATCAAG CGGAGTTCGA TGCGGAGCTG 
GAGGAGCTGA CAAGCACAAG TGTGGAACAC ATCATTGTCA ATCCTAATGC TGCCTATGAC 
AAGTTCAAGG ACAAGAGAGT GGGGACAAAG GGACTTGATT TCTCAGATCG TATTGGAAAA 
ACCAAGAGGA CAGGATATGA ATCTGGAGAA TATGAGATGC TTGGAGAGGG TCTGGGAGTG 
AAGGAGACAC CCCAGCAAAA GTACCAGCGC CTACTGCATG AGGTCCAAGA GCTGACAACT 
GAAGTTGAAA AAATCAAGAC GACAGTGAAG GAGTCAGCCA CAGAGGAGAA GCTGACCCCT 
GTGTTGCTGG CTAAACAGCT GGCAGCCCTG AAGCAGCAGC TGGTTGCTTC CCACCTGGAG 
AAGCTGCTGG GACCAGATGC TGCAATCAAC CTTACCGACC CCGATGGCGC CCTGGCTAAG 
CGCCTACTAC TGCAGCTGGA AGCAACAAAG AACAGCAAAG GGGGATCAGG GGGAAAAACC 
ACTGGGACCC CCCCAGATAG CAGCCTTGTC ACTTATGAAC TACATTCTCG GCCTGAGCAG 
GACAAGTTCT CTCAAGCTGC CAAAGTCGCA GAACTTGAAA AGCGCCTGAC AGAGCTGGAG 
ACAGCTGTAC GTTGTGATCA GGATGCTCAG AATCCCCTTT CTGCAGGTCT ACAGGGAGCC 
TGTCTCATGG AGACTGTAGA GCTGTTGCAA GCAAAGGTGA GCGCCCTAGA CCTTGCAGTT 
TTGGATCAAG TGGAGGCTCG GCTACAGAGT GTCCTGGGAA AGGTGAACGA GATTGCCAAG 
CATAAAGCCT CTGTAGAAGA TGCAGATACA CAAAGCAAGG TGCACCAGCT ATATGAAACT 
ATACAGCGCT GGAGCCCCAT TGCCTCCACC CTCCCTGAGC TGGTGCAGAG ACTTGTCACC 
ATCAAGCAGC TGCACGAGCA AGCCATGCAG TTTGGTCAGC TCCTGACACA CTTGGATACC 
ACCCAGCAGA TGATTGCTAA TTCCTTGAAG GACAATACCA CCCTCTTGAC CCAGGTGCAG 
ACAACCATGC GTGAAAACCT GGCCACAGTT GAGGGGAACT TTGCCAGCAT TGATGAACGG 
ATGAAGAAGC TGGGAAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DCTN2 ( NM_001348066.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001334995.1 Homo sapiens dynactin subunit 2 (DCTN2), transcript variant 5, mRNA.

Price & Availability↑

CloneID	OHu102293
Clone ID Related Accession (Same CDS sequence)	NM_001348066.1
Accession Version	NM_001348066.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1137bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-04
Organism	Homo sapiens(human)
Product	dynactin subunit 2 isoform 5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC022506.38 and AK096242.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK096242.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTATGCAC AGGGAACAGA GGATTCAAGG GAGCTGGAGG AGCTGACAAG CACAAGTGTG 
GAACACATCA TTGTCAATCC TAATGCTGCC TATGACAAGT TCAAGGACAA GAGAGTGGGG 
ACAAAGGGAC TTGATTTCTC AGATCGTATT GGAAAAACCA AGAGGACAGG ATATGAATCT 
GGAGAATATG AGATGCTTGG AGAGGGTCTG GGAGTGAAGG AGACACCCCA GCAAAAGTAC 
CAGCGCCTAC TGCATGAGGT CCAAGAGCTG ACAACTGAAG TTGAAAAAAT CAAGACGACA 
GTGAAGGAGT CAGCCACAGA GGAGAAGCTG ACCCCTGTGT TGCTGGCTAA ACAGCTGGCA 
GCCCTGAAGC AGCAGCTGGT TGCTTCCCAC CTGGAGAAGC TGCTGGGACC AGATGCTGCA 
ATCAACCTTA CCGACCCCGA TGGCGCCCTG GCTAAGCGCC TACTACTGCA GCTGGAAGCA 
ACAAAGAACA GCAAAGGGGG ATCAGGGGGA AAAACCACTG GGACCCCCCC AGATAGCAGC 
CTTGTCACTT ATGAACTACA TTCTCGGCCT GAGCAGGACA AGTTCTCTCA AGCTGCCAAA 
GTCGCAGAAC TTGAAAAGCG CCTGACAGAG CTGGAGACAG CTGTACGTTG TGATCAGGAT 
GCTCAGAATC CCCTTTCTGC AGGTCTACAG GGAGCCTGTC TCATGGAGAC TGTAGAGCTG 
TTGCAAGCAA AGGTGAGCGC CCTAGACCTT GCAGTTTTGG ATCAAGTGGA GGCTCGGCTA 
CAGAGTGTCC TGGGAAAGGT GAACGAGATT GCCAAGCATA AAGCCTCTGT AGAAGATGCA 
GATACACAAA GCAAGGTGCA CCAGCTATAT GAAACTATAC AGCGCTGGAG CCCCATTGCC 
TCCACCCTCC CTGAGCTGGT GCAGAGACTT GTCACCATCA AGCAGCTGCA CGAGCAAGCC 
ATGCAGTTTG GTCAGCTCCT GACACACTTG GATACCACCC AGCAGATGAT TGCTAATTCC 
TTGAAGGACA ATACCACCCT CTTGACCCAG GTGCAGACAA CCATGCGTGA AAACCTGGCC 
ACAGTTGAGG GGAACTTTGC CAGCATTGAT GAACGGATGA AGAAGCTGGG AAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001334995.1
CDS	389..1525
Translation	MYAQGTEDSRELEELTSTSVEHIIVNPNAAYDKFKDKRVGTKGLDFSDRIGKTKRTGYESGEYEMLGEGLGVKETPQQKYQRLLHEVQELTTEVEKIKTTVKESATEEKLTPVLLAKQLAALKQQLVASHLEKLLGPDAAINLTDPDGALAKRLLLQLEATKNSKGGSGGKTTGTPPDSSLVTYELHSRPEQDKFSQAAKVAELEKRLTELETAVRCDQDAQNPLSAGLQGACLMETVELLQAKVSALDLAVLDQVEARLQSVLGKVNEIAKHKASVEDADTQSKVHQLYETIQRWSPIASTLPELVQRLVTIKQLHEQAMQFGQLLTHLDTTQQMIANSLKDNTTLLTQVQTTMRENLATVEGNFASIDERMKKLGK

Target ORF information:

RefSeq Version	NM_001348066.1
Organism	Homo sapiens(human)
Definition	Homo sapiens dynactin subunit 2 (DCTN2), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001348066.1

ORF Insert Sequence:

ATGTATGCAC AGGGAACAGA GGATTCAAGG GAGCTGGAGG AGCTGACAAG CACAAGTGTG 
GAACACATCA TTGTCAATCC TAATGCTGCC TATGACAAGT TCAAGGACAA GAGAGTGGGG 
ACAAAGGGAC TTGATTTCTC AGATCGTATT GGAAAAACCA AGAGGACAGG ATATGAATCT 
GGAGAATATG AGATGCTTGG AGAGGGTCTG GGAGTGAAGG AGACACCCCA GCAAAAGTAC 
CAGCGCCTAC TGCATGAGGT CCAAGAGCTG ACAACTGAAG TTGAAAAAAT CAAGACGACA 
GTGAAGGAGT CAGCCACAGA GGAGAAGCTG ACCCCTGTGT TGCTGGCTAA ACAGCTGGCA 
GCCCTGAAGC AGCAGCTGGT TGCTTCCCAC CTGGAGAAGC TGCTGGGACC AGATGCTGCA 
ATCAACCTTA CCGACCCCGA TGGCGCCCTG GCTAAGCGCC TACTACTGCA GCTGGAAGCA 
ACAAAGAACA GCAAAGGGGG ATCAGGGGGA AAAACCACTG GGACCCCCCC AGATAGCAGC 
CTTGTCACTT ATGAACTACA TTCTCGGCCT GAGCAGGACA AGTTCTCTCA AGCTGCCAAA 
GTCGCAGAAC TTGAAAAGCG CCTGACAGAG CTGGAGACAG CTGTACGTTG TGATCAGGAT 
GCTCAGAATC CCCTTTCTGC AGGTCTACAG GGAGCCTGTC TCATGGAGAC TGTAGAGCTG 
TTGCAAGCAA AGGTGAGCGC CCTAGACCTT GCAGTTTTGG ATCAAGTGGA GGCTCGGCTA 
CAGAGTGTCC TGGGAAAGGT GAACGAGATT GCCAAGCATA AAGCCTCTGT AGAAGATGCA 
GATACACAAA GCAAGGTGCA CCAGCTATAT GAAACTATAC AGCGCTGGAG CCCCATTGCC 
TCCACCCTCC CTGAGCTGGT GCAGAGACTT GTCACCATCA AGCAGCTGCA CGAGCAAGCC 
ATGCAGTTTG GTCAGCTCCT GACACACTTG GATACCACCC AGCAGATGAT TGCTAATTCC 
TTGAAGGACA ATACCACCCT CTTGACCCAG GTGCAGACAA CCATGCGTGA AAACCTGGCC 
ACAGTTGAGG GGAACTTTGC CAGCATTGAT GAACGGATGA AGAAGCTGGG AAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Variants in the genes DCTN2, DNAH10, LRIG3, and MYO1A are associated with intermediate Charcot-Marie-Tooth disease in a Norwegian family.
Acta neurologica Scandinavica134(1)67-75(2016 Jul)
Braathen GJ,H?yer H,Busk ?L,Tveten K,Skjelbred CF,Russell MB

An organelle-specific protein landscape identifies novel diseases and molecular mechanisms.
Nature communications711491(2016 May)
Boldt K,van Reeuwijk J,Lu Q,Koutroumpas K,Nguyen TM,Texier Y,van Beersum SE,Horn N,Willer JR,Mans DA,Dougherty G,Lamers IJ,Coene KL,Arts HH,Betts MJ,Beyer T,Bolat E,Gloeckner CJ,Haidari K,Hetterschijt L,Iaconis D,Jenkins D,Klose F,Knapp B,Latour B,Letteboer SJ,Marcelis CL,Mitic D,Morleo M,Oud MM,Riemersma M,Rix S,Terhal PA,Toedt G,van Dam TJ,de Vrieze E,Wissinger Y,Wu KM,Apic G,Beales PL,Blacque OE,Gibson TJ,Huynen MA,Katsanis N,Kremer H,Omran H,van Wijk E,Wolfrum U,Kepes F,Davis EE,Franco B,Giles RH,Ueffing M,Russell RB,Roepman R,

A molecular mechanism to regulate lysosome motility for lysosome positioning and tubulation.
Nature cell biology18(4)404-17(2016 Apr)
Li X,Rydzewski N,Hider A,Zhang X,Yang J,Wang W,Gao Q,Cheng X,Xu H

Pooled-matrix protein interaction screens using Barcode Fusion Genetics.
Molecular systems biology12(4)863(2016 Apr)
Yachie N,Petsalaki E,Mellor JC,Weile J,Jacob Y,Verby M,Ozturk SB,Li S,Cote AG,Mosca R,Knapp JJ,Ko M,Yu A,Gebbia M,Sahni N,Yi S,Tyagi T,Sheykhkarimli D,Roth JF,Wong C,Musa L,Snider J,Liu YC,Yu H,Braun P,Stagljar I,Hao T,Calderwood MA,Pelletier L,Aloy P,Hill DE,Vidal M,Roth FP

A Dynamic Protein Interaction Landscape of the Human Centrosome-Cilium Interface.
Cell163(6)1484-99(2015 Dec)
Gupta GD,Coyaud ?,Gon?alves J,Mojarad BA,Liu Y,Wu Q,Gheiratmand L,Comartin D,Tkach JM,Cheung SW,Bashkurov M,Hasegan M,Knight JD,Lin ZY,Schueler M,Hildebrandt F,Moffat J,Gingras AC,Raught B,Pelletier L

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DCTN2 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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