ATG7 cDNA ORF clone, Homo sapiens(human)

The following ATG7 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ATG7 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu25208 NM_001136031.3
Latest version!
Homo sapiens autophagy related 7 (ATG7), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$279.50-$391.30
$559.00
OHu25286 NM_001144912.2
Latest version!
Homo sapiens autophagy related 7 (ATG7), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$49.50-$69.30
$99.00
OHu25208 XM_011533286.2
Latest version!
Homo sapiens autophagy related 7 (ATG7), transcript variant X21, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$279.50-$391.30
$559.00
OHu102391 NM_001349238.2
Latest version!
Homo sapiens autophagy related 7 (ATG7), transcript variant 10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00
OHu105298 XM_024453313.1
Latest version!
Homo sapiens autophagy related 7 (ATG7), transcript variant X23, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00
OHu105298 XM_024453314.1
Latest version!
Homo sapiens autophagy related 7 (ATG7), transcript variant X24, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00
OHu93737 XM_024453315.1
Latest version!
Homo sapiens autophagy related 7 (ATG7), transcript variant X25, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00
OHu93737 XM_017005553.1
Latest version!
Homo sapiens autophagy related 7 (ATG7), transcript variant X27, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00
OHu25185 NM_006395.3
Latest version!
Homo sapiens autophagy related 7 (ATG7), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu25185 NM_001349235.2
Latest version!
Homo sapiens autophagy related 7 (ATG7), transcript variant 7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu25185 NM_001349233.2
Latest version!
Homo sapiens autophagy related 7 (ATG7), transcript variant 5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu25185 NM_001349234.2
Latest version!
Homo sapiens autophagy related 7 (ATG7), transcript variant 6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu25185 NM_001349232.2
Latest version!
Homo sapiens autophagy related 7 (ATG7), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu36913 XM_017005542.1
Latest version!
Homo sapiens autophagy related 7 (ATG7), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu36913 XM_006712931.4
Latest version!
Homo sapiens autophagy related 7 (ATG7), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu36913 XM_011533277.3
Latest version!
Homo sapiens autophagy related 7 (ATG7), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu36913 XM_006712933.3
Latest version!
Homo sapiens autophagy related 7 (ATG7), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu36913 XM_011533278.2
Latest version!
Homo sapiens autophagy related 7 (ATG7), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu36913 XM_006712932.4
Latest version!
Homo sapiens autophagy related 7 (ATG7), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu36915 XM_011533283.2
Latest version!
Homo sapiens autophagy related 7 (ATG7), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu36915 XM_017005548.1
Latest version!
Homo sapiens autophagy related 7 (ATG7), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu36917 XM_011533285.3
Latest version!
Homo sapiens autophagy related 7 (ATG7), transcript variant X18, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu36917 XM_017005549.2
Latest version!
Homo sapiens autophagy related 7 (ATG7), transcript variant X19, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu64106 XM_024453312.1
Latest version!
Homo sapiens autophagy related 7 (ATG7), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu64106 XM_011533279.2
Latest version!
Homo sapiens autophagy related 7 (ATG7), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu64107 XM_011533280.2
Latest version!
Homo sapiens autophagy related 7 (ATG7), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu64107 XM_017005543.1
Latest version!
Homo sapiens autophagy related 7 (ATG7), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu64108 XM_011533281.2
Latest version!
Homo sapiens autophagy related 7 (ATG7), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu64109 XM_011533284.2
Latest version!
Homo sapiens autophagy related 7 (ATG7), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu102116 NM_001349236.2
Latest version!
Homo sapiens autophagy related 7 (ATG7), transcript variant 8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
OHu102196 NM_001349237.2
Latest version!
Homo sapiens autophagy related 7 (ATG7), transcript variant 9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
OHu93734 XM_017005547.2
Latest version!
Homo sapiens autophagy related 7 (ATG7), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OHu93735 XM_017005550.1
Latest version!
Homo sapiens autophagy related 7 (ATG7), transcript variant X20, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OHu93736 XM_017005551.1
Latest version!
Homo sapiens autophagy related 7 (ATG7), transcript variant X22, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
View Old Accession Versions >>
OHu36917 XM_011533285.2 Homo sapiens autophagy related 7 (ATG7), transcript variant X21, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu25208 XM_011533286.1 Homo sapiens autophagy related 7 (ATG7), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$279.50-$391.30
$559.00
OHu25208 NM_001136031.2 Homo sapiens autophagy related 7 (ATG7), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$279.50-$391.30
$559.00
OHu36913 XM_006712932.3 Homo sapiens autophagy related 7 (ATG7), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu25185 NM_001349232.1 Homo sapiens autophagy related 7 (ATG7), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu102391 NM_001349238.1 Homo sapiens autophagy related 7 (ATG7), transcript variant 10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00
OHu102196 NM_001349237.1 Homo sapiens autophagy related 7 (ATG7), transcript variant 9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
OHu25286 NM_001144912.1 Homo sapiens autophagy related 7 (ATG7), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$49.50-$69.30
$99.00
OHu36913 XM_006712931.3 Homo sapiens autophagy related 7 (ATG7), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu36917 XM_017005549.1 Homo sapiens autophagy related 7 (ATG7), transcript variant X22, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu25185 NM_001349233.1 Homo sapiens autophagy related 7 (ATG7), transcript variant 5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu25185 NM_001349235.1 Homo sapiens autophagy related 7 (ATG7), transcript variant 7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu25185 NM_001349234.1 Homo sapiens autophagy related 7 (ATG7), transcript variant 6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu36913 XM_011533277.2 Homo sapiens autophagy related 7 (ATG7), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu93734 XM_017005547.1 Homo sapiens autophagy related 7 (ATG7), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OHu102116 NM_001349236.1 Homo sapiens autophagy related 7 (ATG7), transcript variant 8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
OHu25185 NM_006395.2 Homo sapiens autophagy related 7 (ATG7), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu25208
    Clone ID Related Accession (Same CDS sequence) XM_011533286.1 , NM_001136031.3 , XM_011533286.2 , NM_001136031.2
    Accession Version NM_001136031.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2031bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-06-22
    Organism Homo sapiens(human)
    Product ubiquitin-like modifier-activating enzyme ATG7 isoform b
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA177126.1, BC000091.2, AL122075.1 and AC022001.5. On Jun 2, 2019 this sequence version replaced NM_001136031.2. Transcript Variant: This variant (2) encodes isoform b. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.194516.1, SRR1803613.36331.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGCGGCAG CTACGGGGGA TCCTGGACTC TCTAAACTGC AGTTTGCCCC TTTTAGTAGT 
    GCCTTGGATG TTGGGTTTTG GCATGAGTTG ACCCAGAAGA AGCTGAACGA GTATCGGCTG
    GATGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAATG GTGACTCTGC TGGGCTGCCA
    GCTCGCTTAA CATTGGAGTT CAGTGCTTTT GACATGAGTG CTCCCACCCC AGCCCGTTGC
    TGCCCAGCTA TTGGAACACT GTATAACACC AACACACTCG AGTCTTTCAA GACTGCAGAT
    AAGAAGCTCC TTTTGGAACA AGCAGCAAAT GAGATATGGG AATCCATAAA ATCAGGCACT
    GCTCTTGAAA ACCCTGTACT CCTCAACAAG TTCCTCCTCT TGACATTTGC AGATCTAAAG
    AAGTACCACT TCTACTATTG GTTTTGCTAT CCTGCCCTCT GTCTTCCAGA GAGTTTACCT
    CTCATTCAGG GGCCAGTGGG TTTGGATCAA AGGTTTTCAC TAAAACAGAT TGAAGCACTA
    GAGTGTGCAT ATGATAATCT TTGTCAAACA GAAGGAGTCA CAGCTCTTCC TTACTTCTTA
    ATCAAGTATG ATGAGAACAT GGTGCTGGTT TCCTTGCTTA AACACTACAG TGATTTCTTC
    CAAGGTCAAA GGACGAAGAT AACAATTGGT GTATATGATC CCTGTAACTT AGCCCAGTAC
    CCTGGATGGC CTTTGAGGAA TTTTTTGGTC CTAGCAGCCC ACAGATGGAG TAGCAGTTTC
    CAGTCTGTTG AAGTTGTTTG CTTCCGTGAC CGTACCATGC AGGGGGCGAG AGACGTTGCC
    CACAGCATCA TCTTCGAAGT GAAGCTTCCA GAAATGGCAT TTAGCCCAGA TTGTCCTAAA
    GCAGTTGGAT GGGAAAAGAA CCAGAAAGGA GGCATGGGAC CAAGGATGGT GAACCTCAGT
    GAATGTATGG ACCCTAAAAG GTTAGCTGAG TCATCAGTGG ATCTAAATCT CAAACTGATG
    TGTTGGAGAT TGGTTCCTAC TTTAGACTTG GACAAGGTTG TGTCTGTCAA ATGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGCAATGTA GCTAGGACGT TGATGGGTTG GGGCGTGAGA
    CACATCACAT TTGTGGACAA TGCCAAGATC TCCTACTCCA ATCCTGTGAG GCAGCCTCTC
    TATGAGTTTG AAGATTGCCT AGGGGGTGGT AAGCCCAAGG CTCTGGCAGC AGCGGACCGG
    CTCCAGAAAA TATTCCCCGG TGTGAATGCC AGAGGATTCA ACATGAGCAT ACCTATGCCT
    GGGCATCCAG TGAACTTCTC CAGTGTCACT CTGGAGCAAG CCCGCAGAGA TGTGGAGCAA
    CTGGAGCAGC TCATCGAAAG CCATGATGTC GTCTTCCTAT TGATGGACAC CAGGGAGAGC
    CGGTGGCTTC CTGCCGTCAT TGCTGCAAGC AAGAGAAAGC TGGTCATCAA TGCTGCTTTG
    GGATTTGACA CATTTGTTGT CATGAGACAT GGTCTGAAGA AACCAAAGCA GCAAGGAGCT
    GGGGACTTGT GTCCAAACCA CCCTGTGGCA TCTGCTGACC TCCTGGGCTC ATCGCTTTTT
    GCCAACATCC CTGGTTACAA GCTTGGCTGC TACTTCTGCA ATGATGTGGT GGCCCCAGGA
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGTCGTCC AGGACTGGCC
    GTGATTGCAG GAGCCCTGGC CGTGGAATTG ATGGTATCTG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAT CGGATGAATG AGCCTCCAAC CTCTCTTGGG
    CTTGTGCCTC ACCAGGTTCT TGATCAATAT GAACGAGAAG GATTTAACTT CCTAGCCAAG
    GTGTTTAATT CTTCACATTC CTTCTTAGAA GACTTGACTG GTCTTACATT GCTGCATCAA
    GAAACCCAAG CTGCTGAGAT CTGGGACATG AGCGATGATG AGACCATCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001129503.2
    CDS45..2075
    Translation

    Target ORF information:

    RefSeq Version NM_001136031.3
    Organism Homo sapiens(human)
    Definition Homo sapiens autophagy related 7 (ATG7), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001136031.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGCGGCAG CTACGGGGGA TCCTGGACTC TCTAAACTGC AGTTTGCCCC TTTTAGTAGT 
    GCCTTGGATG TTGGGTTTTG GCATGAGTTG ACCCAGAAGA AGCTGAACGA GTATCGGCTG
    GATGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAATG GTGACTCTGC TGGGCTGCCA
    GCTCGCTTAA CATTGGAGTT CAGTGCTTTT GACATGAGTG CTCCCACCCC AGCCCGTTGC
    TGCCCAGCTA TTGGAACACT GTATAACACC AACACACTCG AGTCTTTCAA GACTGCAGAT
    AAGAAGCTCC TTTTGGAACA AGCAGCAAAT GAGATATGGG AATCCATAAA ATCAGGCACT
    GCTCTTGAAA ACCCTGTACT CCTCAACAAG TTCCTCCTCT TGACATTTGC AGATCTAAAG
    AAGTACCACT TCTACTATTG GTTTTGCTAT CCTGCCCTCT GTCTTCCAGA GAGTTTACCT
    CTCATTCAGG GGCCAGTGGG TTTGGATCAA AGGTTTTCAC TAAAACAGAT TGAAGCACTA
    GAGTGTGCAT ATGATAATCT TTGTCAAACA GAAGGAGTCA CAGCTCTTCC TTACTTCTTA
    ATCAAGTATG ATGAGAACAT GGTGCTGGTT TCCTTGCTTA AACACTACAG TGATTTCTTC
    CAAGGTCAAA GGACGAAGAT AACAATTGGT GTATATGATC CCTGTAACTT AGCCCAGTAC
    CCTGGATGGC CTTTGAGGAA TTTTTTGGTC CTAGCAGCCC ACAGATGGAG TAGCAGTTTC
    CAGTCTGTTG AAGTTGTTTG CTTCCGTGAC CGTACCATGC AGGGGGCGAG AGACGTTGCC
    CACAGCATCA TCTTCGAAGT GAAGCTTCCA GAAATGGCAT TTAGCCCAGA TTGTCCTAAA
    GCAGTTGGAT GGGAAAAGAA CCAGAAAGGA GGCATGGGAC CAAGGATGGT GAACCTCAGT
    GAATGTATGG ACCCTAAAAG GTTAGCTGAG TCATCAGTGG ATCTAAATCT CAAACTGATG
    TGTTGGAGAT TGGTTCCTAC TTTAGACTTG GACAAGGTTG TGTCTGTCAA ATGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGCAATGTA GCTAGGACGT TGATGGGTTG GGGCGTGAGA
    CACATCACAT TTGTGGACAA TGCCAAGATC TCCTACTCCA ATCCTGTGAG GCAGCCTCTC
    TATGAGTTTG AAGATTGCCT AGGGGGTGGT AAGCCCAAGG CTCTGGCAGC AGCGGACCGG
    CTCCAGAAAA TATTCCCCGG TGTGAATGCC AGAGGATTCA ACATGAGCAT ACCTATGCCT
    GGGCATCCAG TGAACTTCTC CAGTGTCACT CTGGAGCAAG CCCGCAGAGA TGTGGAGCAA
    CTGGAGCAGC TCATCGAAAG CCATGATGTC GTCTTCCTAT TGATGGACAC CAGGGAGAGC
    CGGTGGCTTC CTGCCGTCAT TGCTGCAAGC AAGAGAAAGC TGGTCATCAA TGCTGCTTTG
    GGATTTGACA CATTTGTTGT CATGAGACAT GGTCTGAAGA AACCAAAGCA GCAAGGAGCT
    GGGGACTTGT GTCCAAACCA CCCTGTGGCA TCTGCTGACC TCCTGGGCTC ATCGCTTTTT
    GCCAACATCC CTGGTTACAA GCTTGGCTGC TACTTCTGCA ATGATGTGGT GGCCCCAGGA
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGTCGTCC AGGACTGGCC
    GTGATTGCAG GAGCCCTGGC CGTGGAATTG ATGGTATCTG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAT CGGATGAATG AGCCTCCAAC CTCTCTTGGG
    CTTGTGCCTC ACCAGGTTCT TGATCAATAT GAACGAGAAG GATTTAACTT CCTAGCCAAG
    GTGTTTAATT CTTCACATTC CTTCTTAGAA GACTTGACTG GTCTTACATT GCTGCATCAA
    GAAACCCAAG CTGCTGAGAT CTGGGACATG AGCGATGATG AGACCATCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu25208
    Clone ID Related Accession (Same CDS sequence) XM_011533286.1 , NM_001136031.3 , XM_011533286.2 , NM_001136031.2
    Accession Version NM_001136031.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2031bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-02
    Organism Homo sapiens(human)
    Product ubiquitin-like modifier-activating enzyme ATG7 isoform b
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA177126.1, AC083855.3, BC000091.2, AL122075.1 and AC022001.5. On Jan 30, 2009 this sequence version replaced NM_001136031.1. Transcript Variant: This variant (2) encodes isoform b. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.194516.1, SRR1803616.261973.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGCGGCAG CTACGGGGGA TCCTGGACTC TCTAAACTGC AGTTTGCCCC TTTTAGTAGT 
    GCCTTGGATG TTGGGTTTTG GCATGAGTTG ACCCAGAAGA AGCTGAACGA GTATCGGCTG
    GATGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAATG GTGACTCTGC TGGGCTGCCA
    GCTCGCTTAA CATTGGAGTT CAGTGCTTTT GACATGAGTG CTCCCACCCC AGCCCGTTGC
    TGCCCAGCTA TTGGAACACT GTATAACACC AACACACTCG AGTCTTTCAA GACTGCAGAT
    AAGAAGCTCC TTTTGGAACA AGCAGCAAAT GAGATATGGG AATCCATAAA ATCAGGCACT
    GCTCTTGAAA ACCCTGTACT CCTCAACAAG TTCCTCCTCT TGACATTTGC AGATCTAAAG
    AAGTACCACT TCTACTATTG GTTTTGCTAT CCTGCCCTCT GTCTTCCAGA GAGTTTACCT
    CTCATTCAGG GGCCAGTGGG TTTGGATCAA AGGTTTTCAC TAAAACAGAT TGAAGCACTA
    GAGTGTGCAT ATGATAATCT TTGTCAAACA GAAGGAGTCA CAGCTCTTCC TTACTTCTTA
    ATCAAGTATG ATGAGAACAT GGTGCTGGTT TCCTTGCTTA AACACTACAG TGATTTCTTC
    CAAGGTCAAA GGACGAAGAT AACAATTGGT GTATATGATC CCTGTAACTT AGCCCAGTAC
    CCTGGATGGC CTTTGAGGAA TTTTTTGGTC CTAGCAGCCC ACAGATGGAG TAGCAGTTTC
    CAGTCTGTTG AAGTTGTTTG CTTCCGTGAC CGTACCATGC AGGGGGCGAG AGACGTTGCC
    CACAGCATCA TCTTCGAAGT GAAGCTTCCA GAAATGGCAT TTAGCCCAGA TTGTCCTAAA
    GCAGTTGGAT GGGAAAAGAA CCAGAAAGGA GGCATGGGAC CAAGGATGGT GAACCTCAGT
    GAATGTATGG ACCCTAAAAG GTTAGCTGAG TCATCAGTGG ATCTAAATCT CAAACTGATG
    TGTTGGAGAT TGGTTCCTAC TTTAGACTTG GACAAGGTTG TGTCTGTCAA ATGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGCAATGTA GCTAGGACGT TGATGGGTTG GGGCGTGAGA
    CACATCACAT TTGTGGACAA TGCCAAGATC TCCTACTCCA ATCCTGTGAG GCAGCCTCTC
    TATGAGTTTG AAGATTGCCT AGGGGGTGGT AAGCCCAAGG CTCTGGCAGC AGCGGACCGG
    CTCCAGAAAA TATTCCCCGG TGTGAATGCC AGAGGATTCA ACATGAGCAT ACCTATGCCT
    GGGCATCCAG TGAACTTCTC CAGTGTCACT CTGGAGCAAG CCCGCAGAGA TGTGGAGCAA
    CTGGAGCAGC TCATCGAAAG CCATGATGTC GTCTTCCTAT TGATGGACAC CAGGGAGAGC
    CGGTGGCTTC CTGCCGTCAT TGCTGCAAGC AAGAGAAAGC TGGTCATCAA TGCTGCTTTG
    GGATTTGACA CATTTGTTGT CATGAGACAT GGTCTGAAGA AACCAAAGCA GCAAGGAGCT
    GGGGACTTGT GTCCAAACCA CCCTGTGGCA TCTGCTGACC TCCTGGGCTC ATCGCTTTTT
    GCCAACATCC CTGGTTACAA GCTTGGCTGC TACTTCTGCA ATGATGTGGT GGCCCCAGGA
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGTCGTCC AGGACTGGCC
    GTGATTGCAG GAGCCCTGGC CGTGGAATTG ATGGTATCTG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAT CGGATGAATG AGCCTCCAAC CTCTCTTGGG
    CTTGTGCCTC ACCAGGTTCT TGATCAATAT GAACGAGAAG GATTTAACTT CCTAGCCAAG
    GTGTTTAATT CTTCACATTC CTTCTTAGAA GACTTGACTG GTCTTACATT GCTGCATCAA
    GAAACCCAAG CTGCTGAGAT CTGGGACATG AGCGATGATG AGACCATCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001129503.2
    CDS118..2148
    Translation

    Target ORF information:

    RefSeq Version NM_001136031.2
    Organism Homo sapiens(human)
    Definition Homo sapiens autophagy related 7 (ATG7), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001136031.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGCGGCAG CTACGGGGGA TCCTGGACTC TCTAAACTGC AGTTTGCCCC TTTTAGTAGT 
    GCCTTGGATG TTGGGTTTTG GCATGAGTTG ACCCAGAAGA AGCTGAACGA GTATCGGCTG
    GATGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAATG GTGACTCTGC TGGGCTGCCA
    GCTCGCTTAA CATTGGAGTT CAGTGCTTTT GACATGAGTG CTCCCACCCC AGCCCGTTGC
    TGCCCAGCTA TTGGAACACT GTATAACACC AACACACTCG AGTCTTTCAA GACTGCAGAT
    AAGAAGCTCC TTTTGGAACA AGCAGCAAAT GAGATATGGG AATCCATAAA ATCAGGCACT
    GCTCTTGAAA ACCCTGTACT CCTCAACAAG TTCCTCCTCT TGACATTTGC AGATCTAAAG
    AAGTACCACT TCTACTATTG GTTTTGCTAT CCTGCCCTCT GTCTTCCAGA GAGTTTACCT
    CTCATTCAGG GGCCAGTGGG TTTGGATCAA AGGTTTTCAC TAAAACAGAT TGAAGCACTA
    GAGTGTGCAT ATGATAATCT TTGTCAAACA GAAGGAGTCA CAGCTCTTCC TTACTTCTTA
    ATCAAGTATG ATGAGAACAT GGTGCTGGTT TCCTTGCTTA AACACTACAG TGATTTCTTC
    CAAGGTCAAA GGACGAAGAT AACAATTGGT GTATATGATC CCTGTAACTT AGCCCAGTAC
    CCTGGATGGC CTTTGAGGAA TTTTTTGGTC CTAGCAGCCC ACAGATGGAG TAGCAGTTTC
    CAGTCTGTTG AAGTTGTTTG CTTCCGTGAC CGTACCATGC AGGGGGCGAG AGACGTTGCC
    CACAGCATCA TCTTCGAAGT GAAGCTTCCA GAAATGGCAT TTAGCCCAGA TTGTCCTAAA
    GCAGTTGGAT GGGAAAAGAA CCAGAAAGGA GGCATGGGAC CAAGGATGGT GAACCTCAGT
    GAATGTATGG ACCCTAAAAG GTTAGCTGAG TCATCAGTGG ATCTAAATCT CAAACTGATG
    TGTTGGAGAT TGGTTCCTAC TTTAGACTTG GACAAGGTTG TGTCTGTCAA ATGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGCAATGTA GCTAGGACGT TGATGGGTTG GGGCGTGAGA
    CACATCACAT TTGTGGACAA TGCCAAGATC TCCTACTCCA ATCCTGTGAG GCAGCCTCTC
    TATGAGTTTG AAGATTGCCT AGGGGGTGGT AAGCCCAAGG CTCTGGCAGC AGCGGACCGG
    CTCCAGAAAA TATTCCCCGG TGTGAATGCC AGAGGATTCA ACATGAGCAT ACCTATGCCT
    GGGCATCCAG TGAACTTCTC CAGTGTCACT CTGGAGCAAG CCCGCAGAGA TGTGGAGCAA
    CTGGAGCAGC TCATCGAAAG CCATGATGTC GTCTTCCTAT TGATGGACAC CAGGGAGAGC
    CGGTGGCTTC CTGCCGTCAT TGCTGCAAGC AAGAGAAAGC TGGTCATCAA TGCTGCTTTG
    GGATTTGACA CATTTGTTGT CATGAGACAT GGTCTGAAGA AACCAAAGCA GCAAGGAGCT
    GGGGACTTGT GTCCAAACCA CCCTGTGGCA TCTGCTGACC TCCTGGGCTC ATCGCTTTTT
    GCCAACATCC CTGGTTACAA GCTTGGCTGC TACTTCTGCA ATGATGTGGT GGCCCCAGGA
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGTCGTCC AGGACTGGCC
    GTGATTGCAG GAGCCCTGGC CGTGGAATTG ATGGTATCTG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAT CGGATGAATG AGCCTCCAAC CTCTCTTGGG
    CTTGTGCCTC ACCAGGTTCT TGATCAATAT GAACGAGAAG GATTTAACTT CCTAGCCAAG
    GTGTTTAATT CTTCACATTC CTTCTTAGAA GACTTGACTG GTCTTACATT GCTGCATCAA
    GAAACCCAAG CTGCTGAGAT CTGGGACATG AGCGATGATG AGACCATCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu25286
    Clone ID Related Accession (Same CDS sequence) NM_001144912.2 , NM_001144912.1
    Accession Version NM_001144912.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1872bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-06-22
    Organism Homo sapiens(human)
    Product ubiquitin-like modifier-activating enzyme ATG7 isoform c
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA177126.1, BC000091.2, AK303694.1, AL122075.1 and AC022001.5. On Jun 2, 2019 this sequence version replaced NM_001144912.1. Transcript Variant: This variant (3) encodes isoform c. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK303694.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03465404 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGCGGCAG CTACGGGGGA TCCTGGACTC TCTAAACTGC AGTTTGCCCC TTTTAGTAGT 
    GCCTTGGATG TTGGGTTTTG GCATGAGTTG ACCCAGAAGA AGCTGAACGA GTATCGGCTG
    GATGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAATG GTGACTCTGC TGGGCTGCCA
    GCTCGCTTAA CATTGGAGTT CAGTGCTTTT GACATGAGTG CTCCCACCCC AGCCCGTTGC
    TGCCCAGCTA TTGGAACACT GTATAACACC AACACACTCG AGTCTTTCAA GACTGCAGAT
    AAGAAGCTCC TTTTGGAACA AGCAGCAAAT GAGATATGGG AATCCATAAA ATCAGGCACT
    GCTCTTGAAA ACCCTGTACT CCTCAACAAG TTCCTCCTCT TGACATTTGC AATTGAAGCA
    CTAGAGTGTG CATATGATAA TCTTTGTCAA ACAGAAGGAG TCACAGCTCT TCCTTACTTC
    TTAATCAAGT ATGATGAGAA CATGGTGCTG GTTTCCTTGC TTAAACACTA CAGTGATTTC
    TTCCAAGGTC AAAGGACGAA GATAACAATT GGTGTATATG ATCCCTGTAA CTTAGCCCAG
    TACCCTGGAT GGCCTTTGAG GAATTTTTTG GTCCTAGCAG CCCACAGATG GAGTAGCAGT
    TTCCAGTCTG TTGAAGTTGT TTGCTTCCGT GACCGTACCA TGCAGGGGGC GAGAGACGTT
    GCCCACAGCA TCATCTTCGA AGTGAAGCTT CCAGAAATGG CATTTAGCCC AGATTGTCCT
    AAAGCAGTTG GATGGGAAAA GAACCAGAAA GGAGGCATGG GACCAAGGAT GGTGAACCTC
    AGTGAATGTA TGGACCCTAA AAGGTTAGCT GAGTCATCAG TGGATCTAAA TCTCAAACTG
    ATGTGTTGGA GATTGGTTCC TACTTTAGAC TTGGACAAGG TTGTGTCTGT CAAATGTCTG
    CTGCTTGGAG CCGGCACCTT GGGTTGCAAT GTAGCTAGGA CGTTGATGGG TTGGGGCGTG
    AGACACATCA CATTTGTGGA CAATGCCAAG ATCTCCTACT CCAATCCTGT GAGGCAGCCT
    CTCTATGAGT TTGAAGATTG CCTAGGGGGT GGTAAGCCCA AGGCTCTGGC AGCAGCGGAC
    CGGCTCCAGA AAATATTCCC CGGTGTGAAT GCCAGAGGAT TCAACATGAG CATACCTATG
    CCTGGGCATC CAGTGAACTT CTCCAGTGTC ACTCTGGAGC AAGCCCGCAG AGATGTGGAG
    CAACTGGAGC AGCTCATCGA AAGCCATGAT GTCGTCTTCC TATTGATGGA CACCAGGGAG
    AGCCGGTGGC TTCCTGCCGT CATTGCTGCA AGCAAGAGAA AGCTGGTCAT CAATGCTGCT
    TTGGGATTTG ACACATTTGT TGTCATGAGA CATGGTCTGA AGAAACCAAA GCAGCAAGGA
    GCTGGGGACT TGTGTCCAAA CCACCCTGTG GCATCTGCTG ACCTCCTGGG CTCATCGCTT
    TTTGCCAACA TCCCTGGTTA CAAGCTTGGC TGCTACTTCT GCAATGATGT GGTGGCCCCA
    GGAGATTCAA CCAGAGACCG GACCTTGGAC CAGCAGTGCA CTGTGAGTCG TCCAGGACTG
    GCCGTGATTG CAGGAGCCCT GGCCGTGGAA TTGATGGTAT CTGTTTTGCA GCATCCAGAA
    GGGGGCTATG CCATTGCCAG CAGCAGTGAC GATCGGATGA ATGAGCCTCC AACCTCTCTT
    GGGCTTGTGC CTCACCAGAT CCGGGGATTT CTTTCACGGT TTGATAATGT CCTTCCCGTC
    AGCCTGGCAT TTGACAAATG TACAGCTTGT TCTTCCAAAA TCTGGGACAT GAGCGATGAT
    GAGACCATCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001138384.1
    CDS45..1916
    Translation

    Target ORF information:

    RefSeq Version NM_001144912.2
    Organism Homo sapiens(human)
    Definition Homo sapiens autophagy related 7 (ATG7), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001144912.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGCGGCAG CTACGGGGGA TCCTGGACTC TCTAAACTGC AGTTTGCCCC TTTTAGTAGT 
    GCCTTGGATG TTGGGTTTTG GCATGAGTTG ACCCAGAAGA AGCTGAACGA GTATCGGCTG
    GATGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAATG GTGACTCTGC TGGGCTGCCA
    GCTCGCTTAA CATTGGAGTT CAGTGCTTTT GACATGAGTG CTCCCACCCC AGCCCGTTGC
    TGCCCAGCTA TTGGAACACT GTATAACACC AACACACTCG AGTCTTTCAA GACTGCAGAT
    AAGAAGCTCC TTTTGGAACA AGCAGCAAAT GAGATATGGG AATCCATAAA ATCAGGCACT
    GCTCTTGAAA ACCCTGTACT CCTCAACAAG TTCCTCCTCT TGACATTTGC AATTGAAGCA
    CTAGAGTGTG CATATGATAA TCTTTGTCAA ACAGAAGGAG TCACAGCTCT TCCTTACTTC
    TTAATCAAGT ATGATGAGAA CATGGTGCTG GTTTCCTTGC TTAAACACTA CAGTGATTTC
    TTCCAAGGTC AAAGGACGAA GATAACAATT GGTGTATATG ATCCCTGTAA CTTAGCCCAG
    TACCCTGGAT GGCCTTTGAG GAATTTTTTG GTCCTAGCAG CCCACAGATG GAGTAGCAGT
    TTCCAGTCTG TTGAAGTTGT TTGCTTCCGT GACCGTACCA TGCAGGGGGC GAGAGACGTT
    GCCCACAGCA TCATCTTCGA AGTGAAGCTT CCAGAAATGG CATTTAGCCC AGATTGTCCT
    AAAGCAGTTG GATGGGAAAA GAACCAGAAA GGAGGCATGG GACCAAGGAT GGTGAACCTC
    AGTGAATGTA TGGACCCTAA AAGGTTAGCT GAGTCATCAG TGGATCTAAA TCTCAAACTG
    ATGTGTTGGA GATTGGTTCC TACTTTAGAC TTGGACAAGG TTGTGTCTGT CAAATGTCTG
    CTGCTTGGAG CCGGCACCTT GGGTTGCAAT GTAGCTAGGA CGTTGATGGG TTGGGGCGTG
    AGACACATCA CATTTGTGGA CAATGCCAAG ATCTCCTACT CCAATCCTGT GAGGCAGCCT
    CTCTATGAGT TTGAAGATTG CCTAGGGGGT GGTAAGCCCA AGGCTCTGGC AGCAGCGGAC
    CGGCTCCAGA AAATATTCCC CGGTGTGAAT GCCAGAGGAT TCAACATGAG CATACCTATG
    CCTGGGCATC CAGTGAACTT CTCCAGTGTC ACTCTGGAGC AAGCCCGCAG AGATGTGGAG
    CAACTGGAGC AGCTCATCGA AAGCCATGAT GTCGTCTTCC TATTGATGGA CACCAGGGAG
    AGCCGGTGGC TTCCTGCCGT CATTGCTGCA AGCAAGAGAA AGCTGGTCAT CAATGCTGCT
    TTGGGATTTG ACACATTTGT TGTCATGAGA CATGGTCTGA AGAAACCAAA GCAGCAAGGA
    GCTGGGGACT TGTGTCCAAA CCACCCTGTG GCATCTGCTG ACCTCCTGGG CTCATCGCTT
    TTTGCCAACA TCCCTGGTTA CAAGCTTGGC TGCTACTTCT GCAATGATGT GGTGGCCCCA
    GGAGATTCAA CCAGAGACCG GACCTTGGAC CAGCAGTGCA CTGTGAGTCG TCCAGGACTG
    GCCGTGATTG CAGGAGCCCT GGCCGTGGAA TTGATGGTAT CTGTTTTGCA GCATCCAGAA
    GGGGGCTATG CCATTGCCAG CAGCAGTGAC GATCGGATGA ATGAGCCTCC AACCTCTCTT
    GGGCTTGTGC CTCACCAGAT CCGGGGATTT CTTTCACGGT TTGATAATGT CCTTCCCGTC
    AGCCTGGCAT TTGACAAATG TACAGCTTGT TCTTCCAAAA TCTGGGACAT GAGCGATGAT
    GAGACCATCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu25286
    Clone ID Related Accession (Same CDS sequence) NM_001144912.2 , NM_001144912.1
    Accession Version NM_001144912.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1872bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-02
    Organism Homo sapiens(human)
    Product ubiquitin-like modifier-activating enzyme ATG7 isoform c
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA177126.1, AC083855.3, BC000091.2, AK303694.1, AL122075.1 and AC022001.5. Transcript Variant: This variant (3) encodes isoform c. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK303694.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03465404 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGCGGCAG CTACGGGGGA TCCTGGACTC TCTAAACTGC AGTTTGCCCC TTTTAGTAGT 
    GCCTTGGATG TTGGGTTTTG GCATGAGTTG ACCCAGAAGA AGCTGAACGA GTATCGGCTG
    GATGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAATG GTGACTCTGC TGGGCTGCCA
    GCTCGCTTAA CATTGGAGTT CAGTGCTTTT GACATGAGTG CTCCCACCCC AGCCCGTTGC
    TGCCCAGCTA TTGGAACACT GTATAACACC AACACACTCG AGTCTTTCAA GACTGCAGAT
    AAGAAGCTCC TTTTGGAACA AGCAGCAAAT GAGATATGGG AATCCATAAA ATCAGGCACT
    GCTCTTGAAA ACCCTGTACT CCTCAACAAG TTCCTCCTCT TGACATTTGC AATTGAAGCA
    CTAGAGTGTG CATATGATAA TCTTTGTCAA ACAGAAGGAG TCACAGCTCT TCCTTACTTC
    TTAATCAAGT ATGATGAGAA CATGGTGCTG GTTTCCTTGC TTAAACACTA CAGTGATTTC
    TTCCAAGGTC AAAGGACGAA GATAACAATT GGTGTATATG ATCCCTGTAA CTTAGCCCAG
    TACCCTGGAT GGCCTTTGAG GAATTTTTTG GTCCTAGCAG CCCACAGATG GAGTAGCAGT
    TTCCAGTCTG TTGAAGTTGT TTGCTTCCGT GACCGTACCA TGCAGGGGGC GAGAGACGTT
    GCCCACAGCA TCATCTTCGA AGTGAAGCTT CCAGAAATGG CATTTAGCCC AGATTGTCCT
    AAAGCAGTTG GATGGGAAAA GAACCAGAAA GGAGGCATGG GACCAAGGAT GGTGAACCTC
    AGTGAATGTA TGGACCCTAA AAGGTTAGCT GAGTCATCAG TGGATCTAAA TCTCAAACTG
    ATGTGTTGGA GATTGGTTCC TACTTTAGAC TTGGACAAGG TTGTGTCTGT CAAATGTCTG
    CTGCTTGGAG CCGGCACCTT GGGTTGCAAT GTAGCTAGGA CGTTGATGGG TTGGGGCGTG
    AGACACATCA CATTTGTGGA CAATGCCAAG ATCTCCTACT CCAATCCTGT GAGGCAGCCT
    CTCTATGAGT TTGAAGATTG CCTAGGGGGT GGTAAGCCCA AGGCTCTGGC AGCAGCGGAC
    CGGCTCCAGA AAATATTCCC CGGTGTGAAT GCCAGAGGAT TCAACATGAG CATACCTATG
    CCTGGGCATC CAGTGAACTT CTCCAGTGTC ACTCTGGAGC AAGCCCGCAG AGATGTGGAG
    CAACTGGAGC AGCTCATCGA AAGCCATGAT GTCGTCTTCC TATTGATGGA CACCAGGGAG
    AGCCGGTGGC TTCCTGCCGT CATTGCTGCA AGCAAGAGAA AGCTGGTCAT CAATGCTGCT
    TTGGGATTTG ACACATTTGT TGTCATGAGA CATGGTCTGA AGAAACCAAA GCAGCAAGGA
    GCTGGGGACT TGTGTCCAAA CCACCCTGTG GCATCTGCTG ACCTCCTGGG CTCATCGCTT
    TTTGCCAACA TCCCTGGTTA CAAGCTTGGC TGCTACTTCT GCAATGATGT GGTGGCCCCA
    GGAGATTCAA CCAGAGACCG GACCTTGGAC CAGCAGTGCA CTGTGAGTCG TCCAGGACTG
    GCCGTGATTG CAGGAGCCCT GGCCGTGGAA TTGATGGTAT CTGTTTTGCA GCATCCAGAA
    GGGGGCTATG CCATTGCCAG CAGCAGTGAC GATCGGATGA ATGAGCCTCC AACCTCTCTT
    GGGCTTGTGC CTCACCAGAT CCGGGGATTT CTTTCACGGT TTGATAATGT CCTTCCCGTC
    AGCCTGGCAT TTGACAAATG TACAGCTTGT TCTTCCAAAA TCTGGGACAT GAGCGATGAT
    GAGACCATCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001138384.1
    CDS118..1989
    Translation

    Target ORF information:

    RefSeq Version NM_001144912.1
    Organism Homo sapiens(human)
    Definition Homo sapiens autophagy related 7 (ATG7), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001144912.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGCGGCAG CTACGGGGGA TCCTGGACTC TCTAAACTGC AGTTTGCCCC TTTTAGTAGT 
    GCCTTGGATG TTGGGTTTTG GCATGAGTTG ACCCAGAAGA AGCTGAACGA GTATCGGCTG
    GATGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAATG GTGACTCTGC TGGGCTGCCA
    GCTCGCTTAA CATTGGAGTT CAGTGCTTTT GACATGAGTG CTCCCACCCC AGCCCGTTGC
    TGCCCAGCTA TTGGAACACT GTATAACACC AACACACTCG AGTCTTTCAA GACTGCAGAT
    AAGAAGCTCC TTTTGGAACA AGCAGCAAAT GAGATATGGG AATCCATAAA ATCAGGCACT
    GCTCTTGAAA ACCCTGTACT CCTCAACAAG TTCCTCCTCT TGACATTTGC AATTGAAGCA
    CTAGAGTGTG CATATGATAA TCTTTGTCAA ACAGAAGGAG TCACAGCTCT TCCTTACTTC
    TTAATCAAGT ATGATGAGAA CATGGTGCTG GTTTCCTTGC TTAAACACTA CAGTGATTTC
    TTCCAAGGTC AAAGGACGAA GATAACAATT GGTGTATATG ATCCCTGTAA CTTAGCCCAG
    TACCCTGGAT GGCCTTTGAG GAATTTTTTG GTCCTAGCAG CCCACAGATG GAGTAGCAGT
    TTCCAGTCTG TTGAAGTTGT TTGCTTCCGT GACCGTACCA TGCAGGGGGC GAGAGACGTT
    GCCCACAGCA TCATCTTCGA AGTGAAGCTT CCAGAAATGG CATTTAGCCC AGATTGTCCT
    AAAGCAGTTG GATGGGAAAA GAACCAGAAA GGAGGCATGG GACCAAGGAT GGTGAACCTC
    AGTGAATGTA TGGACCCTAA AAGGTTAGCT GAGTCATCAG TGGATCTAAA TCTCAAACTG
    ATGTGTTGGA GATTGGTTCC TACTTTAGAC TTGGACAAGG TTGTGTCTGT CAAATGTCTG
    CTGCTTGGAG CCGGCACCTT GGGTTGCAAT GTAGCTAGGA CGTTGATGGG TTGGGGCGTG
    AGACACATCA CATTTGTGGA CAATGCCAAG ATCTCCTACT CCAATCCTGT GAGGCAGCCT
    CTCTATGAGT TTGAAGATTG CCTAGGGGGT GGTAAGCCCA AGGCTCTGGC AGCAGCGGAC
    CGGCTCCAGA AAATATTCCC CGGTGTGAAT GCCAGAGGAT TCAACATGAG CATACCTATG
    CCTGGGCATC CAGTGAACTT CTCCAGTGTC ACTCTGGAGC AAGCCCGCAG AGATGTGGAG
    CAACTGGAGC AGCTCATCGA AAGCCATGAT GTCGTCTTCC TATTGATGGA CACCAGGGAG
    AGCCGGTGGC TTCCTGCCGT CATTGCTGCA AGCAAGAGAA AGCTGGTCAT CAATGCTGCT
    TTGGGATTTG ACACATTTGT TGTCATGAGA CATGGTCTGA AGAAACCAAA GCAGCAAGGA
    GCTGGGGACT TGTGTCCAAA CCACCCTGTG GCATCTGCTG ACCTCCTGGG CTCATCGCTT
    TTTGCCAACA TCCCTGGTTA CAAGCTTGGC TGCTACTTCT GCAATGATGT GGTGGCCCCA
    GGAGATTCAA CCAGAGACCG GACCTTGGAC CAGCAGTGCA CTGTGAGTCG TCCAGGACTG
    GCCGTGATTG CAGGAGCCCT GGCCGTGGAA TTGATGGTAT CTGTTTTGCA GCATCCAGAA
    GGGGGCTATG CCATTGCCAG CAGCAGTGAC GATCGGATGA ATGAGCCTCC AACCTCTCTT
    GGGCTTGTGC CTCACCAGAT CCGGGGATTT CTTTCACGGT TTGATAATGT CCTTCCCGTC
    AGCCTGGCAT TTGACAAATG TACAGCTTGT TCTTCCAAAA TCTGGGACAT GAGCGATGAT
    GAGACCATCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu25208
    Clone ID Related Accession (Same CDS sequence) XM_011533286.1 , NM_001136031.3 , XM_011533286.2 , NM_001136031.2
    Accession Version XM_011533286.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2031bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-03-11
    Organism Homo sapiens(Human)
    Product ubiquitin-like modifier-activating enzyme ATG7 isoform X2
    Comment

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGCGGCAG CTACGGGGGA TCCTGGACTC TCTAAACTGC AGTTTGCCCC TTTTAGTAGT 
    GCCTTGGATG TTGGGTTTTG GCATGAGTTG ACCCAGAAGA AGCTGAACGA GTATCGGCTG
    GATGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAATG GTGACTCTGC TGGGCTGCCA
    GCTCGCTTAA CATTGGAGTT CAGTGCTTTT GACATGAGTG CTCCCACCCC AGCCCGTTGC
    TGCCCAGCTA TTGGAACACT GTATAACACC AACACACTCG AGTCTTTCAA GACTGCAGAT
    AAGAAGCTCC TTTTGGAACA AGCAGCAAAT GAGATATGGG AATCCATAAA ATCAGGCACT
    GCTCTTGAAA ACCCTGTACT CCTCAACAAG TTCCTCCTCT TGACATTTGC AGATCTAAAG
    AAGTACCACT TCTACTATTG GTTTTGCTAT CCTGCCCTCT GTCTTCCAGA GAGTTTACCT
    CTCATTCAGG GGCCAGTGGG TTTGGATCAA AGGTTTTCAC TAAAACAGAT TGAAGCACTA
    GAGTGTGCAT ATGATAATCT TTGTCAAACA GAAGGAGTCA CAGCTCTTCC TTACTTCTTA
    ATCAAGTATG ATGAGAACAT GGTGCTGGTT TCCTTGCTTA AACACTACAG TGATTTCTTC
    CAAGGTCAAA GGACGAAGAT AACAATTGGT GTATATGATC CCTGTAACTT AGCCCAGTAC
    CCTGGATGGC CTTTGAGGAA TTTTTTGGTC CTAGCAGCCC ACAGATGGAG TAGCAGTTTC
    CAGTCTGTTG AAGTTGTTTG CTTCCGTGAC CGTACCATGC AGGGGGCGAG AGACGTTGCC
    CACAGCATCA TCTTCGAAGT GAAGCTTCCA GAAATGGCAT TTAGCCCAGA TTGTCCTAAA
    GCAGTTGGAT GGGAAAAGAA CCAGAAAGGA GGCATGGGAC CAAGGATGGT GAACCTCAGT
    GAATGTATGG ACCCTAAAAG GTTAGCTGAG TCATCAGTGG ATCTAAATCT CAAACTGATG
    TGTTGGAGAT TGGTTCCTAC TTTAGACTTG GACAAGGTTG TGTCTGTCAA ATGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGCAATGTA GCTAGGACGT TGATGGGTTG GGGCGTGAGA
    CACATCACAT TTGTGGACAA TGCCAAGATC TCCTACTCCA ATCCTGTGAG GCAGCCTCTC
    TATGAGTTTG AAGATTGCCT AGGGGGTGGT AAGCCCAAGG CTCTGGCAGC AGCGGACCGG
    CTCCAGAAAA TATTCCCCGG TGTGAATGCC AGAGGATTCA ACATGAGCAT ACCTATGCCT
    GGGCATCCAG TGAACTTCTC CAGTGTCACT CTGGAGCAAG CCCGCAGAGA TGTGGAGCAA
    CTGGAGCAGC TCATCGAAAG CCATGATGTC GTCTTCCTAT TGATGGACAC CAGGGAGAGC
    CGGTGGCTTC CTGCCGTCAT TGCTGCAAGC AAGAGAAAGC TGGTCATCAA TGCTGCTTTG
    GGATTTGACA CATTTGTTGT CATGAGACAT GGTCTGAAGA AACCAAAGCA GCAAGGAGCT
    GGGGACTTGT GTCCAAACCA CCCTGTGGCA TCTGCTGACC TCCTGGGCTC ATCGCTTTTT
    GCCAACATCC CTGGTTACAA GCTTGGCTGC TACTTCTGCA ATGATGTGGT GGCCCCAGGA
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGTCGTCC AGGACTGGCC
    GTGATTGCAG GAGCCCTGGC CGTGGAATTG ATGGTATCTG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAT CGGATGAATG AGCCTCCAAC CTCTCTTGGG
    CTTGTGCCTC ACCAGGTTCT TGATCAATAT GAACGAGAAG GATTTAACTT CCTAGCCAAG
    GTGTTTAATT CTTCACATTC CTTCTTAGAA GACTTGACTG GTCTTACATT GCTGCATCAA
    GAAACCCAAG CTGCTGAGAT CTGGGACATG AGCGATGATG AGACCATCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011531588.1
    Translation

    Target ORF information:

    RefSeq Version XM_011533286.1
    Organism Homo sapiens(Human)
    Definition Homo sapiens autophagy related 7 (ATG7), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011533286.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGCGGCAG CTACGGGGGA TCCTGGACTC TCTAAACTGC AGTTTGCCCC TTTTAGTAGT 
    GCCTTGGATG TTGGGTTTTG GCATGAGTTG ACCCAGAAGA AGCTGAACGA GTATCGGCTG
    GATGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAATG GTGACTCTGC TGGGCTGCCA
    GCTCGCTTAA CATTGGAGTT CAGTGCTTTT GACATGAGTG CTCCCACCCC AGCCCGTTGC
    TGCCCAGCTA TTGGAACACT GTATAACACC AACACACTCG AGTCTTTCAA GACTGCAGAT
    AAGAAGCTCC TTTTGGAACA AGCAGCAAAT GAGATATGGG AATCCATAAA ATCAGGCACT
    GCTCTTGAAA ACCCTGTACT CCTCAACAAG TTCCTCCTCT TGACATTTGC AGATCTAAAG
    AAGTACCACT TCTACTATTG GTTTTGCTAT CCTGCCCTCT GTCTTCCAGA GAGTTTACCT
    CTCATTCAGG GGCCAGTGGG TTTGGATCAA AGGTTTTCAC TAAAACAGAT TGAAGCACTA
    GAGTGTGCAT ATGATAATCT TTGTCAAACA GAAGGAGTCA CAGCTCTTCC TTACTTCTTA
    ATCAAGTATG ATGAGAACAT GGTGCTGGTT TCCTTGCTTA AACACTACAG TGATTTCTTC
    CAAGGTCAAA GGACGAAGAT AACAATTGGT GTATATGATC CCTGTAACTT AGCCCAGTAC
    CCTGGATGGC CTTTGAGGAA TTTTTTGGTC CTAGCAGCCC ACAGATGGAG TAGCAGTTTC
    CAGTCTGTTG AAGTTGTTTG CTTCCGTGAC CGTACCATGC AGGGGGCGAG AGACGTTGCC
    CACAGCATCA TCTTCGAAGT GAAGCTTCCA GAAATGGCAT TTAGCCCAGA TTGTCCTAAA
    GCAGTTGGAT GGGAAAAGAA CCAGAAAGGA GGCATGGGAC CAAGGATGGT GAACCTCAGT
    GAATGTATGG ACCCTAAAAG GTTAGCTGAG TCATCAGTGG ATCTAAATCT CAAACTGATG
    TGTTGGAGAT TGGTTCCTAC TTTAGACTTG GACAAGGTTG TGTCTGTCAA ATGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGCAATGTA GCTAGGACGT TGATGGGTTG GGGCGTGAGA
    CACATCACAT TTGTGGACAA TGCCAAGATC TCCTACTCCA ATCCTGTGAG GCAGCCTCTC
    TATGAGTTTG AAGATTGCCT AGGGGGTGGT AAGCCCAAGG CTCTGGCAGC AGCGGACCGG
    CTCCAGAAAA TATTCCCCGG TGTGAATGCC AGAGGATTCA ACATGAGCAT ACCTATGCCT
    GGGCATCCAG TGAACTTCTC CAGTGTCACT CTGGAGCAAG CCCGCAGAGA TGTGGAGCAA
    CTGGAGCAGC TCATCGAAAG CCATGATGTC GTCTTCCTAT TGATGGACAC CAGGGAGAGC
    CGGTGGCTTC CTGCCGTCAT TGCTGCAAGC AAGAGAAAGC TGGTCATCAA TGCTGCTTTG
    GGATTTGACA CATTTGTTGT CATGAGACAT GGTCTGAAGA AACCAAAGCA GCAAGGAGCT
    GGGGACTTGT GTCCAAACCA CCCTGTGGCA TCTGCTGACC TCCTGGGCTC ATCGCTTTTT
    GCCAACATCC CTGGTTACAA GCTTGGCTGC TACTTCTGCA ATGATGTGGT GGCCCCAGGA
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGTCGTCC AGGACTGGCC
    GTGATTGCAG GAGCCCTGGC CGTGGAATTG ATGGTATCTG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAT CGGATGAATG AGCCTCCAAC CTCTCTTGGG
    CTTGTGCCTC ACCAGGTTCT TGATCAATAT GAACGAGAAG GATTTAACTT CCTAGCCAAG
    GTGTTTAATT CTTCACATTC CTTCTTAGAA GACTTGACTG GTCTTACATT GCTGCATCAA
    GAAACCCAAG CTGCTGAGAT CTGGGACATG AGCGATGATG AGACCATCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu25208
    Clone ID Related Accession (Same CDS sequence) XM_011533286.1 , NM_001136031.3 , XM_011533286.2 , NM_001136031.2
    Accession Version XM_011533286.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2031bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product ubiquitin-like modifier-activating enzyme ATG7 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011533286.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGCGGCAG CTACGGGGGA TCCTGGACTC TCTAAACTGC AGTTTGCCCC TTTTAGTAGT 
    GCCTTGGATG TTGGGTTTTG GCATGAGTTG ACCCAGAAGA AGCTGAACGA GTATCGGCTG
    GATGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAATG GTGACTCTGC TGGGCTGCCA
    GCTCGCTTAA CATTGGAGTT CAGTGCTTTT GACATGAGTG CTCCCACCCC AGCCCGTTGC
    TGCCCAGCTA TTGGAACACT GTATAACACC AACACACTCG AGTCTTTCAA GACTGCAGAT
    AAGAAGCTCC TTTTGGAACA AGCAGCAAAT GAGATATGGG AATCCATAAA ATCAGGCACT
    GCTCTTGAAA ACCCTGTACT CCTCAACAAG TTCCTCCTCT TGACATTTGC AGATCTAAAG
    AAGTACCACT TCTACTATTG GTTTTGCTAT CCTGCCCTCT GTCTTCCAGA GAGTTTACCT
    CTCATTCAGG GGCCAGTGGG TTTGGATCAA AGGTTTTCAC TAAAACAGAT TGAAGCACTA
    GAGTGTGCAT ATGATAATCT TTGTCAAACA GAAGGAGTCA CAGCTCTTCC TTACTTCTTA
    ATCAAGTATG ATGAGAACAT GGTGCTGGTT TCCTTGCTTA AACACTACAG TGATTTCTTC
    CAAGGTCAAA GGACGAAGAT AACAATTGGT GTATATGATC CCTGTAACTT AGCCCAGTAC
    CCTGGATGGC CTTTGAGGAA TTTTTTGGTC CTAGCAGCCC ACAGATGGAG TAGCAGTTTC
    CAGTCTGTTG AAGTTGTTTG CTTCCGTGAC CGTACCATGC AGGGGGCGAG AGACGTTGCC
    CACAGCATCA TCTTCGAAGT GAAGCTTCCA GAAATGGCAT TTAGCCCAGA TTGTCCTAAA
    GCAGTTGGAT GGGAAAAGAA CCAGAAAGGA GGCATGGGAC CAAGGATGGT GAACCTCAGT
    GAATGTATGG ACCCTAAAAG GTTAGCTGAG TCATCAGTGG ATCTAAATCT CAAACTGATG
    TGTTGGAGAT TGGTTCCTAC TTTAGACTTG GACAAGGTTG TGTCTGTCAA ATGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGCAATGTA GCTAGGACGT TGATGGGTTG GGGCGTGAGA
    CACATCACAT TTGTGGACAA TGCCAAGATC TCCTACTCCA ATCCTGTGAG GCAGCCTCTC
    TATGAGTTTG AAGATTGCCT AGGGGGTGGT AAGCCCAAGG CTCTGGCAGC AGCGGACCGG
    CTCCAGAAAA TATTCCCCGG TGTGAATGCC AGAGGATTCA ACATGAGCAT ACCTATGCCT
    GGGCATCCAG TGAACTTCTC CAGTGTCACT CTGGAGCAAG CCCGCAGAGA TGTGGAGCAA
    CTGGAGCAGC TCATCGAAAG CCATGATGTC GTCTTCCTAT TGATGGACAC CAGGGAGAGC
    CGGTGGCTTC CTGCCGTCAT TGCTGCAAGC AAGAGAAAGC TGGTCATCAA TGCTGCTTTG
    GGATTTGACA CATTTGTTGT CATGAGACAT GGTCTGAAGA AACCAAAGCA GCAAGGAGCT
    GGGGACTTGT GTCCAAACCA CCCTGTGGCA TCTGCTGACC TCCTGGGCTC ATCGCTTTTT
    GCCAACATCC CTGGTTACAA GCTTGGCTGC TACTTCTGCA ATGATGTGGT GGCCCCAGGA
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGTCGTCC AGGACTGGCC
    GTGATTGCAG GAGCCCTGGC CGTGGAATTG ATGGTATCTG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAT CGGATGAATG AGCCTCCAAC CTCTCTTGGG
    CTTGTGCCTC ACCAGGTTCT TGATCAATAT GAACGAGAAG GATTTAACTT CCTAGCCAAG
    GTGTTTAATT CTTCACATTC CTTCTTAGAA GACTTGACTG GTCTTACATT GCTGCATCAA
    GAAACCCAAG CTGCTGAGAT CTGGGACATG AGCGATGATG AGACCATCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011531588.1
    CDS449..2479
    Translation

    Target ORF information:

    RefSeq Version XM_011533286.2
    Organism Homo sapiens(human)
    Definition Homo sapiens autophagy related 7 (ATG7), transcript variant X21, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011533286.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGCGGCAG CTACGGGGGA TCCTGGACTC TCTAAACTGC AGTTTGCCCC TTTTAGTAGT 
    GCCTTGGATG TTGGGTTTTG GCATGAGTTG ACCCAGAAGA AGCTGAACGA GTATCGGCTG
    GATGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAATG GTGACTCTGC TGGGCTGCCA
    GCTCGCTTAA CATTGGAGTT CAGTGCTTTT GACATGAGTG CTCCCACCCC AGCCCGTTGC
    TGCCCAGCTA TTGGAACACT GTATAACACC AACACACTCG AGTCTTTCAA GACTGCAGAT
    AAGAAGCTCC TTTTGGAACA AGCAGCAAAT GAGATATGGG AATCCATAAA ATCAGGCACT
    GCTCTTGAAA ACCCTGTACT CCTCAACAAG TTCCTCCTCT TGACATTTGC AGATCTAAAG
    AAGTACCACT TCTACTATTG GTTTTGCTAT CCTGCCCTCT GTCTTCCAGA GAGTTTACCT
    CTCATTCAGG GGCCAGTGGG TTTGGATCAA AGGTTTTCAC TAAAACAGAT TGAAGCACTA
    GAGTGTGCAT ATGATAATCT TTGTCAAACA GAAGGAGTCA CAGCTCTTCC TTACTTCTTA
    ATCAAGTATG ATGAGAACAT GGTGCTGGTT TCCTTGCTTA AACACTACAG TGATTTCTTC
    CAAGGTCAAA GGACGAAGAT AACAATTGGT GTATATGATC CCTGTAACTT AGCCCAGTAC
    CCTGGATGGC CTTTGAGGAA TTTTTTGGTC CTAGCAGCCC ACAGATGGAG TAGCAGTTTC
    CAGTCTGTTG AAGTTGTTTG CTTCCGTGAC CGTACCATGC AGGGGGCGAG AGACGTTGCC
    CACAGCATCA TCTTCGAAGT GAAGCTTCCA GAAATGGCAT TTAGCCCAGA TTGTCCTAAA
    GCAGTTGGAT GGGAAAAGAA CCAGAAAGGA GGCATGGGAC CAAGGATGGT GAACCTCAGT
    GAATGTATGG ACCCTAAAAG GTTAGCTGAG TCATCAGTGG ATCTAAATCT CAAACTGATG
    TGTTGGAGAT TGGTTCCTAC TTTAGACTTG GACAAGGTTG TGTCTGTCAA ATGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGCAATGTA GCTAGGACGT TGATGGGTTG GGGCGTGAGA
    CACATCACAT TTGTGGACAA TGCCAAGATC TCCTACTCCA ATCCTGTGAG GCAGCCTCTC
    TATGAGTTTG AAGATTGCCT AGGGGGTGGT AAGCCCAAGG CTCTGGCAGC AGCGGACCGG
    CTCCAGAAAA TATTCCCCGG TGTGAATGCC AGAGGATTCA ACATGAGCAT ACCTATGCCT
    GGGCATCCAG TGAACTTCTC CAGTGTCACT CTGGAGCAAG CCCGCAGAGA TGTGGAGCAA
    CTGGAGCAGC TCATCGAAAG CCATGATGTC GTCTTCCTAT TGATGGACAC CAGGGAGAGC
    CGGTGGCTTC CTGCCGTCAT TGCTGCAAGC AAGAGAAAGC TGGTCATCAA TGCTGCTTTG
    GGATTTGACA CATTTGTTGT CATGAGACAT GGTCTGAAGA AACCAAAGCA GCAAGGAGCT
    GGGGACTTGT GTCCAAACCA CCCTGTGGCA TCTGCTGACC TCCTGGGCTC ATCGCTTTTT
    GCCAACATCC CTGGTTACAA GCTTGGCTGC TACTTCTGCA ATGATGTGGT GGCCCCAGGA
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGTCGTCC AGGACTGGCC
    GTGATTGCAG GAGCCCTGGC CGTGGAATTG ATGGTATCTG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAT CGGATGAATG AGCCTCCAAC CTCTCTTGGG
    CTTGTGCCTC ACCAGGTTCT TGATCAATAT GAACGAGAAG GATTTAACTT CCTAGCCAAG
    GTGTTTAATT CTTCACATTC CTTCTTAGAA GACTTGACTG GTCTTACATT GCTGCATCAA
    GAAACCCAAG CTGCTGAGAT CTGGGACATG AGCGATGATG AGACCATCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu102391
    Clone ID Related Accession (Same CDS sequence) NM_001349238.2 , NM_001349238.1
    Accession Version NM_001349238.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1173bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-06-22
    Organism Homo sapiens(human)
    Product ubiquitin-like modifier-activating enzyme ATG7 isoform f
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC083855.3, AC020750.4, AC026185.4 and AC022001.5. On Jun 2, 2019 this sequence version replaced NM_001349238.1. Transcript Variant: This variant (10) encodes isoform f. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.242150.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2148874, SAMEA2162841 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGGGACCAA GGATGGTGAA CCTCAGTGAA TGTATGGACC CTAAAAGGTT AGCTGAGTCA 
    TCAGTGGATC TAAATCTCAA ACTGATGTGT TGGAGATTGG TTCCTACTTT AGACTTGGAC
    AAGGTTGTGT CTGTCAAATG TCTGCTGCTT GGAGCCGGCA CCTTGGGTTG CAATGTAGCT
    AGGACGTTGA TGGGTTGGGG CGTGAGACAC ATCACATTTG TGGACAATGC CAAGATCTCC
    TACTCCAATC CTGTGAGGCA GCCTCTCTAT GAGTTTGAAG ATTGCCTAGG GGGTGGTAAG
    CCCAAGGCTC TGGCAGCAGC GGACCGGCTC CAGAAAATAT TCCCCGGTGT GAATGCCAGA
    GGATTCAACA TGAGCATACC TATGCCTGGG CATCCAGTGA ACTTCTCCAG TGTCACTCTG
    GAGCAAGCCC GCAGAGATGT GGAGCAACTG GAGCAGCTCA TCGAAAGCCA TGATGTCGTC
    TTCCTATTGA TGGACACCAG GGAGAGCCGG TGGCTTCCTG CCGTCATTGC TGCAAGCAAG
    AGAAAGCTGG TCATCAATGC TGCTTTGGGA TTTGACACAT TTGTTGTCAT GAGACATGGT
    CTGAAGAAAC CAAAGCAGCA AGGAGCTGGG GACTTGTGTC CAAACCACCC TGTGGCATCT
    GCTGACCTCC TGGGCTCATC GCTTTTTGCC AACATCCCTG GTTACAAGCT TGGCTGCTAC
    TTCTGCAATG ATGTGGTGGC CCCAGGAGAT TCAACCAGAG ACCGGACCTT GGACCAGCAG
    TGCACTGTGA GTCGTCCAGG ACTGGCCGTG ATTGCAGGAG CCCTGGCCGT GGAATTGATG
    GTATCTGTTT TGCAGCATCC AGAAGGGGGC TATGCCATTG CCAGCAGCAG TGACGATCGG
    ATGAATGAGC CTCCAACCTC TCTTGGGCTT GTGCCTCACC AGATCCGGGG ATTTCTTTCA
    CGGTTTGATA ATGTCCTTCC CGTCAGCCTG GCATTTGACA AATGTACAGC TTGTTCTTCC
    AAAGTTCTTG ATCAATATGA ACGAGAAGGA TTTAACTTCC TAGCCAAGGT GTTTAATTCT
    TCACATTCCT TCTTAGAAGA CTTGACTGGT CTTACATTGC TGCATCAAGA AACCCAAGCT
    GCTGAGAAAA AAATCCCTAT GTACCAATCA TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001336167.1
    CDS856..2028
    Translation

    Target ORF information:

    RefSeq Version NM_001349238.2
    Organism Homo sapiens(human)
    Definition Homo sapiens autophagy related 7 (ATG7), transcript variant 10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001349238.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGGGACCAA GGATGGTGAA CCTCAGTGAA TGTATGGACC CTAAAAGGTT AGCTGAGTCA 
    TCAGTGGATC TAAATCTCAA ACTGATGTGT TGGAGATTGG TTCCTACTTT AGACTTGGAC
    AAGGTTGTGT CTGTCAAATG TCTGCTGCTT GGAGCCGGCA CCTTGGGTTG CAATGTAGCT
    AGGACGTTGA TGGGTTGGGG CGTGAGACAC ATCACATTTG TGGACAATGC CAAGATCTCC
    TACTCCAATC CTGTGAGGCA GCCTCTCTAT GAGTTTGAAG ATTGCCTAGG GGGTGGTAAG
    CCCAAGGCTC TGGCAGCAGC GGACCGGCTC CAGAAAATAT TCCCCGGTGT GAATGCCAGA
    GGATTCAACA TGAGCATACC TATGCCTGGG CATCCAGTGA ACTTCTCCAG TGTCACTCTG
    GAGCAAGCCC GCAGAGATGT GGAGCAACTG GAGCAGCTCA TCGAAAGCCA TGATGTCGTC
    TTCCTATTGA TGGACACCAG GGAGAGCCGG TGGCTTCCTG CCGTCATTGC TGCAAGCAAG
    AGAAAGCTGG TCATCAATGC TGCTTTGGGA TTTGACACAT TTGTTGTCAT GAGACATGGT
    CTGAAGAAAC CAAAGCAGCA AGGAGCTGGG GACTTGTGTC CAAACCACCC TGTGGCATCT
    GCTGACCTCC TGGGCTCATC GCTTTTTGCC AACATCCCTG GTTACAAGCT TGGCTGCTAC
    TTCTGCAATG ATGTGGTGGC CCCAGGAGAT TCAACCAGAG ACCGGACCTT GGACCAGCAG
    TGCACTGTGA GTCGTCCAGG ACTGGCCGTG ATTGCAGGAG CCCTGGCCGT GGAATTGATG
    GTATCTGTTT TGCAGCATCC AGAAGGGGGC TATGCCATTG CCAGCAGCAG TGACGATCGG
    ATGAATGAGC CTCCAACCTC TCTTGGGCTT GTGCCTCACC AGATCCGGGG ATTTCTTTCA
    CGGTTTGATA ATGTCCTTCC CGTCAGCCTG GCATTTGACA AATGTACAGC TTGTTCTTCC
    AAAGTTCTTG ATCAATATGA ACGAGAAGGA TTTAACTTCC TAGCCAAGGT GTTTAATTCT
    TCACATTCCT TCTTAGAAGA CTTGACTGGT CTTACATTGC TGCATCAAGA AACCCAAGCT
    GCTGAGAAAA AAATCCCTAT GTACCAATCA TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu102391
    Clone ID Related Accession (Same CDS sequence) NM_001349238.2 , NM_001349238.1
    Accession Version NM_001349238.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1173bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-02
    Organism Homo sapiens(human)
    Product ubiquitin-like modifier-activating enzyme ATG7 isoform f
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC083855.3, AC020750.4, AC026185.4 and AC022001.5. On Mar 9, 2017 this sequence version replaced XM_017005554.1. Transcript Variant: This variant (10) encodes isoform f. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.242150.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2148874, SAMEA2162841 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGGGACCAA GGATGGTGAA CCTCAGTGAA TGTATGGACC CTAAAAGGTT AGCTGAGTCA 
    TCAGTGGATC TAAATCTCAA ACTGATGTGT TGGAGATTGG TTCCTACTTT AGACTTGGAC
    AAGGTTGTGT CTGTCAAATG TCTGCTGCTT GGAGCCGGCA CCTTGGGTTG CAATGTAGCT
    AGGACGTTGA TGGGTTGGGG CGTGAGACAC ATCACATTTG TGGACAATGC CAAGATCTCC
    TACTCCAATC CTGTGAGGCA GCCTCTCTAT GAGTTTGAAG ATTGCCTAGG GGGTGGTAAG
    CCCAAGGCTC TGGCAGCAGC GGACCGGCTC CAGAAAATAT TCCCCGGTGT GAATGCCAGA
    GGATTCAACA TGAGCATACC TATGCCTGGG CATCCAGTGA ACTTCTCCAG TGTCACTCTG
    GAGCAAGCCC GCAGAGATGT GGAGCAACTG GAGCAGCTCA TCGAAAGCCA TGATGTCGTC
    TTCCTATTGA TGGACACCAG GGAGAGCCGG TGGCTTCCTG CCGTCATTGC TGCAAGCAAG
    AGAAAGCTGG TCATCAATGC TGCTTTGGGA TTTGACACAT TTGTTGTCAT GAGACATGGT
    CTGAAGAAAC CAAAGCAGCA AGGAGCTGGG GACTTGTGTC CAAACCACCC TGTGGCATCT
    GCTGACCTCC TGGGCTCATC GCTTTTTGCC AACATCCCTG GTTACAAGCT TGGCTGCTAC
    TTCTGCAATG ATGTGGTGGC CCCAGGAGAT TCAACCAGAG ACCGGACCTT GGACCAGCAG
    TGCACTGTGA GTCGTCCAGG ACTGGCCGTG ATTGCAGGAG CCCTGGCCGT GGAATTGATG
    GTATCTGTTT TGCAGCATCC AGAAGGGGGC TATGCCATTG CCAGCAGCAG TGACGATCGG
    ATGAATGAGC CTCCAACCTC TCTTGGGCTT GTGCCTCACC AGATCCGGGG ATTTCTTTCA
    CGGTTTGATA ATGTCCTTCC CGTCAGCCTG GCATTTGACA AATGTACAGC TTGTTCTTCC
    AAAGTTCTTG ATCAATATGA ACGAGAAGGA TTTAACTTCC TAGCCAAGGT GTTTAATTCT
    TCACATTCCT TCTTAGAAGA CTTGACTGGT CTTACATTGC TGCATCAAGA AACCCAAGCT
    GCTGAGAAAA AAATCCCTAT GTACCAATCA TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001336167.1
    CDS929..2101
    Translation

    Target ORF information:

    RefSeq Version NM_001349238.1
    Organism Homo sapiens(human)
    Definition Homo sapiens autophagy related 7 (ATG7), transcript variant 10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001349238.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGGGACCAA GGATGGTGAA CCTCAGTGAA TGTATGGACC CTAAAAGGTT AGCTGAGTCA 
    TCAGTGGATC TAAATCTCAA ACTGATGTGT TGGAGATTGG TTCCTACTTT AGACTTGGAC
    AAGGTTGTGT CTGTCAAATG TCTGCTGCTT GGAGCCGGCA CCTTGGGTTG CAATGTAGCT
    AGGACGTTGA TGGGTTGGGG CGTGAGACAC ATCACATTTG TGGACAATGC CAAGATCTCC
    TACTCCAATC CTGTGAGGCA GCCTCTCTAT GAGTTTGAAG ATTGCCTAGG GGGTGGTAAG
    CCCAAGGCTC TGGCAGCAGC GGACCGGCTC CAGAAAATAT TCCCCGGTGT GAATGCCAGA
    GGATTCAACA TGAGCATACC TATGCCTGGG CATCCAGTGA ACTTCTCCAG TGTCACTCTG
    GAGCAAGCCC GCAGAGATGT GGAGCAACTG GAGCAGCTCA TCGAAAGCCA TGATGTCGTC
    TTCCTATTGA TGGACACCAG GGAGAGCCGG TGGCTTCCTG CCGTCATTGC TGCAAGCAAG
    AGAAAGCTGG TCATCAATGC TGCTTTGGGA TTTGACACAT TTGTTGTCAT GAGACATGGT
    CTGAAGAAAC CAAAGCAGCA AGGAGCTGGG GACTTGTGTC CAAACCACCC TGTGGCATCT
    GCTGACCTCC TGGGCTCATC GCTTTTTGCC AACATCCCTG GTTACAAGCT TGGCTGCTAC
    TTCTGCAATG ATGTGGTGGC CCCAGGAGAT TCAACCAGAG ACCGGACCTT GGACCAGCAG
    TGCACTGTGA GTCGTCCAGG ACTGGCCGTG ATTGCAGGAG CCCTGGCCGT GGAATTGATG
    GTATCTGTTT TGCAGCATCC AGAAGGGGGC TATGCCATTG CCAGCAGCAG TGACGATCGG
    ATGAATGAGC CTCCAACCTC TCTTGGGCTT GTGCCTCACC AGATCCGGGG ATTTCTTTCA
    CGGTTTGATA ATGTCCTTCC CGTCAGCCTG GCATTTGACA AATGTACAGC TTGTTCTTCC
    AAAGTTCTTG ATCAATATGA ACGAGAAGGA TTTAACTTCC TAGCCAAGGT GTTTAATTCT
    TCACATTCCT TCTTAGAAGA CTTGACTGGT CTTACATTGC TGCATCAAGA AACCCAAGCT
    GCTGAGAAAA AAATCCCTAT GTACCAATCA TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu105298
    Clone ID Related Accession (Same CDS sequence) XM_024453314.1 , XM_024453313.1
    Accession Version XM_024453314.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1284bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product ubiquitin-like modifier-activating enzyme ATG7 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGGGACCAA GGATGGTGAA CCTCAGTGAA TGTATGGACC CTAAAAGGTT AGCTGAGTCA 
    TCAGTGGATC TAAATCTCAA ACTGATGTGT TGGAGATTGG TTCCTACTTT AGACTTGGAC
    AAGGTTGTGT CTGTCAAATG TCTGCTGCTT GGAGCCGGCA CCTTGGGTTG CAATGTAGCT
    AGGACGTTGA TGGGTTGGGG CGTGAGACAC ATCACATTTG TGGACAATGC CAAGATCTCC
    TACTCCAATC CTGTGAGGCA GCCTCTCTAT GAGTTTGAAG ATTGCCTAGG GGGTGGTAAG
    CCCAAGGCTC TGGCAGCAGC GGACCGGCTC CAGAAAATAT TCCCCGGTGT GAATGCCAGA
    GGATTCAACA TGAGCATACC TATGCCTGGG CATCCAGTGA ACTTCTCCAG TGTCACTCTG
    GAGCAAGCCC GCAGAGATGT GGAGCAACTG GAGCAGCTCA TCGAAAGCCA TGATGTCGTC
    TTCCTATTGA TGGACACCAG GGAGAGCCGG TGGCTTCCTG CCGTCATTGC TGCAAGCAAG
    AGAAAGCTGG TCATCAATGC TGCTTTGGGA TTTGACACAT TTGTTGTCAT GAGACATGGT
    CTGAAGAAAC CAAAGCAGCA AGGAGCTGGG GACTTGTGTC CAAACCACCC TGTGGCATCT
    GCTGACCTCC TGGGCTCATC GCTTTTTGCC AACATCCCTG GTTACAAGCT TGGCTGCTAC
    TTCTGCAATG ATGTGGTGGC CCCAGGAGAT TCAACCAGAG ACCGGACCTT GGACCAGCAG
    TGCACTGTGA GTCGTCCAGG ACTGGCCGTG ATTGCAGGAG CCCTGGCCGT GGAATTGATG
    GTATCTGTTT TGCAGCATCC AGAAGGGGGC TATGCCATTG CCAGCAGCAG TGACGATCGG
    ATGAATGAGC CTCCAACCTC TCTTGGGCTT GTGCCTCACC AGATCCGGGG ATTTCTTTCA
    CGGTTTGATA ATGTCCTTCC CGTCAGCCTG GCATTTGACA AATGTACAGC TTGTTCTTCC
    AAAGTTCTTG ATCAATATGA ACGAGAAGGA TTTAACTTCC TAGCCAAGGT GTTTAATTCT
    TCACATTCCT TCTTAGAAGA CTTGACTGGT CTTACATTGC TGCATCAAGA AACCCAAGCT
    GCTGAGGATC ATCTTTCTTT CATCCGGCCC AAGAATGGCA CCTTCCCCGC CCATCAGGCC
    ACCTGCACTC AGGAGTCGGC TGCCTGTCCT GAAATAGATC TCTACCAGCT CTCAAGCAGA
    ACAATTCCTT GCATTCATAC TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024309082.1
    CDS761..2044
    Translation

    Target ORF information:

    RefSeq Version XM_024453314.1
    Organism Homo sapiens(human)
    Definition Homo sapiens autophagy related 7 (ATG7), transcript variant X24, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024453314.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGGGACCAA GGATGGTGAA CCTCAGTGAA TGTATGGACC CTAAAAGGTT AGCTGAGTCA 
    TCAGTGGATC TAAATCTCAA ACTGATGTGT TGGAGATTGG TTCCTACTTT AGACTTGGAC
    AAGGTTGTGT CTGTCAAATG TCTGCTGCTT GGAGCCGGCA CCTTGGGTTG CAATGTAGCT
    AGGACGTTGA TGGGTTGGGG CGTGAGACAC ATCACATTTG TGGACAATGC CAAGATCTCC
    TACTCCAATC CTGTGAGGCA GCCTCTCTAT GAGTTTGAAG ATTGCCTAGG GGGTGGTAAG
    CCCAAGGCTC TGGCAGCAGC GGACCGGCTC CAGAAAATAT TCCCCGGTGT GAATGCCAGA
    GGATTCAACA TGAGCATACC TATGCCTGGG CATCCAGTGA ACTTCTCCAG TGTCACTCTG
    GAGCAAGCCC GCAGAGATGT GGAGCAACTG GAGCAGCTCA TCGAAAGCCA TGATGTCGTC
    TTCCTATTGA TGGACACCAG GGAGAGCCGG TGGCTTCCTG CCGTCATTGC TGCAAGCAAG
    AGAAAGCTGG TCATCAATGC TGCTTTGGGA TTTGACACAT TTGTTGTCAT GAGACATGGT
    CTGAAGAAAC CAAAGCAGCA AGGAGCTGGG GACTTGTGTC CAAACCACCC TGTGGCATCT
    GCTGACCTCC TGGGCTCATC GCTTTTTGCC AACATCCCTG GTTACAAGCT TGGCTGCTAC
    TTCTGCAATG ATGTGGTGGC CCCAGGAGAT TCAACCAGAG ACCGGACCTT GGACCAGCAG
    TGCACTGTGA GTCGTCCAGG ACTGGCCGTG ATTGCAGGAG CCCTGGCCGT GGAATTGATG
    GTATCTGTTT TGCAGCATCC AGAAGGGGGC TATGCCATTG CCAGCAGCAG TGACGATCGG
    ATGAATGAGC CTCCAACCTC TCTTGGGCTT GTGCCTCACC AGATCCGGGG ATTTCTTTCA
    CGGTTTGATA ATGTCCTTCC CGTCAGCCTG GCATTTGACA AATGTACAGC TTGTTCTTCC
    AAAGTTCTTG ATCAATATGA ACGAGAAGGA TTTAACTTCC TAGCCAAGGT GTTTAATTCT
    TCACATTCCT TCTTAGAAGA CTTGACTGGT CTTACATTGC TGCATCAAGA AACCCAAGCT
    GCTGAGGATC ATCTTTCTTT CATCCGGCCC AAGAATGGCA CCTTCCCCGC CCATCAGGCC
    ACCTGCACTC AGGAGTCGGC TGCCTGTCCT GAAATAGATC TCTACCAGCT CTCAAGCAGA
    ACAATTCCTT GCATTCATAC TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu105298
    Clone ID Related Accession (Same CDS sequence) XM_024453314.1 , XM_024453313.1
    Accession Version XM_024453313.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1284bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product ubiquitin-like modifier-activating enzyme ATG7 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGGGACCAA GGATGGTGAA CCTCAGTGAA TGTATGGACC CTAAAAGGTT AGCTGAGTCA 
    TCAGTGGATC TAAATCTCAA ACTGATGTGT TGGAGATTGG TTCCTACTTT AGACTTGGAC
    AAGGTTGTGT CTGTCAAATG TCTGCTGCTT GGAGCCGGCA CCTTGGGTTG CAATGTAGCT
    AGGACGTTGA TGGGTTGGGG CGTGAGACAC ATCACATTTG TGGACAATGC CAAGATCTCC
    TACTCCAATC CTGTGAGGCA GCCTCTCTAT GAGTTTGAAG ATTGCCTAGG GGGTGGTAAG
    CCCAAGGCTC TGGCAGCAGC GGACCGGCTC CAGAAAATAT TCCCCGGTGT GAATGCCAGA
    GGATTCAACA TGAGCATACC TATGCCTGGG CATCCAGTGA ACTTCTCCAG TGTCACTCTG
    GAGCAAGCCC GCAGAGATGT GGAGCAACTG GAGCAGCTCA TCGAAAGCCA TGATGTCGTC
    TTCCTATTGA TGGACACCAG GGAGAGCCGG TGGCTTCCTG CCGTCATTGC TGCAAGCAAG
    AGAAAGCTGG TCATCAATGC TGCTTTGGGA TTTGACACAT TTGTTGTCAT GAGACATGGT
    CTGAAGAAAC CAAAGCAGCA AGGAGCTGGG GACTTGTGTC CAAACCACCC TGTGGCATCT
    GCTGACCTCC TGGGCTCATC GCTTTTTGCC AACATCCCTG GTTACAAGCT TGGCTGCTAC
    TTCTGCAATG ATGTGGTGGC CCCAGGAGAT TCAACCAGAG ACCGGACCTT GGACCAGCAG
    TGCACTGTGA GTCGTCCAGG ACTGGCCGTG ATTGCAGGAG CCCTGGCCGT GGAATTGATG
    GTATCTGTTT TGCAGCATCC AGAAGGGGGC TATGCCATTG CCAGCAGCAG TGACGATCGG
    ATGAATGAGC CTCCAACCTC TCTTGGGCTT GTGCCTCACC AGATCCGGGG ATTTCTTTCA
    CGGTTTGATA ATGTCCTTCC CGTCAGCCTG GCATTTGACA AATGTACAGC TTGTTCTTCC
    AAAGTTCTTG ATCAATATGA ACGAGAAGGA TTTAACTTCC TAGCCAAGGT GTTTAATTCT
    TCACATTCCT TCTTAGAAGA CTTGACTGGT CTTACATTGC TGCATCAAGA AACCCAAGCT
    GCTGAGGATC ATCTTTCTTT CATCCGGCCC AAGAATGGCA CCTTCCCCGC CCATCAGGCC
    ACCTGCACTC AGGAGTCGGC TGCCTGTCCT GAAATAGATC TCTACCAGCT CTCAAGCAGA
    ACAATTCCTT GCATTCATAC TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024309081.1
    CDS624..1907
    Translation

    Target ORF information:

    RefSeq Version XM_024453313.1
    Organism Homo sapiens(human)
    Definition Homo sapiens autophagy related 7 (ATG7), transcript variant X23, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024453313.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGGGACCAA GGATGGTGAA CCTCAGTGAA TGTATGGACC CTAAAAGGTT AGCTGAGTCA 
    TCAGTGGATC TAAATCTCAA ACTGATGTGT TGGAGATTGG TTCCTACTTT AGACTTGGAC
    AAGGTTGTGT CTGTCAAATG TCTGCTGCTT GGAGCCGGCA CCTTGGGTTG CAATGTAGCT
    AGGACGTTGA TGGGTTGGGG CGTGAGACAC ATCACATTTG TGGACAATGC CAAGATCTCC
    TACTCCAATC CTGTGAGGCA GCCTCTCTAT GAGTTTGAAG ATTGCCTAGG GGGTGGTAAG
    CCCAAGGCTC TGGCAGCAGC GGACCGGCTC CAGAAAATAT TCCCCGGTGT GAATGCCAGA
    GGATTCAACA TGAGCATACC TATGCCTGGG CATCCAGTGA ACTTCTCCAG TGTCACTCTG
    GAGCAAGCCC GCAGAGATGT GGAGCAACTG GAGCAGCTCA TCGAAAGCCA TGATGTCGTC
    TTCCTATTGA TGGACACCAG GGAGAGCCGG TGGCTTCCTG CCGTCATTGC TGCAAGCAAG
    AGAAAGCTGG TCATCAATGC TGCTTTGGGA TTTGACACAT TTGTTGTCAT GAGACATGGT
    CTGAAGAAAC CAAAGCAGCA AGGAGCTGGG GACTTGTGTC CAAACCACCC TGTGGCATCT
    GCTGACCTCC TGGGCTCATC GCTTTTTGCC AACATCCCTG GTTACAAGCT TGGCTGCTAC
    TTCTGCAATG ATGTGGTGGC CCCAGGAGAT TCAACCAGAG ACCGGACCTT GGACCAGCAG
    TGCACTGTGA GTCGTCCAGG ACTGGCCGTG ATTGCAGGAG CCCTGGCCGT GGAATTGATG
    GTATCTGTTT TGCAGCATCC AGAAGGGGGC TATGCCATTG CCAGCAGCAG TGACGATCGG
    ATGAATGAGC CTCCAACCTC TCTTGGGCTT GTGCCTCACC AGATCCGGGG ATTTCTTTCA
    CGGTTTGATA ATGTCCTTCC CGTCAGCCTG GCATTTGACA AATGTACAGC TTGTTCTTCC
    AAAGTTCTTG ATCAATATGA ACGAGAAGGA TTTAACTTCC TAGCCAAGGT GTTTAATTCT
    TCACATTCCT TCTTAGAAGA CTTGACTGGT CTTACATTGC TGCATCAAGA AACCCAAGCT
    GCTGAGGATC ATCTTTCTTT CATCCGGCCC AAGAATGGCA CCTTCCCCGC CCATCAGGCC
    ACCTGCACTC AGGAGTCGGC TGCCTGTCCT GAAATAGATC TCTACCAGCT CTCAAGCAGA
    ACAATTCCTT GCATTCATAC TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu93737
    Clone ID Related Accession (Same CDS sequence) XM_017005553.1 , XM_024453315.1
    Accession Version XM_017005553.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1179bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product ubiquitin-like modifier-activating enzyme ATG7 isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGGGACCAA GGATGGTGAA CCTCAGTGAA TGTATGGACC CTAAAAGGTT AGCTGAGTCA 
    TCAGTGGATC TAAATCTCAA ACTGATGTGT TGGAGATTGG TTCCTACTTT AGACTTGGAC
    AAGGTTGTGT CTGTCAAATG TCTGCTGCTT GGAGCCGGCA CCTTGGGTTG CAATGTAGCT
    AGGACGTTGA TGGGTTGGGG CGTGAGACAC ATCACATTTG TGGACAATGC CAAGATCTCC
    TACTCCAATC CTGTGAGGCA GCCTCTCTAT GAGTTTGAAG ATTGCCTAGG GGGTGGTAAG
    CCCAAGGCTC TGGCAGCAGC GGACCGGCTC CAGAAAATAT TCCCCGGTGT GAATGCCAGA
    GGATTCAACA TGAGCATACC TATGCCTGGG CATCCAGTGA ACTTCTCCAG TGTCACTCTG
    GAGCAAGCCC GCAGAGATGT GGAGCAACTG GAGCAGCTCA TCGAAAGCCA TGATGTCGTC
    TTCCTATTGA TGGACACCAG GGAGAGCCGG TGGCTTCCTG CCGTCATTGC TGCAAGCAAG
    AGAAAGCTGG TCATCAATGC TGCTTTGGGA TTTGACACAT TTGTTGTCAT GAGACATGGT
    CTGAAGAAAC CAAAGCAGCA AGGAGCTGGG GACTTGTGTC CAAACCACCC TGTGGCATCT
    GCTGACCTCC TGGGCTCATC GCTTTTTGCC AACATCCCTG GTTACAAGCT TGGCTGCTAC
    TTCTGCAATG ATGTGGTGGC CCCAGGAGAT TCAACCAGAG ACCGGACCTT GGACCAGCAG
    TGCACTGTGA GTCGTCCAGG ACTGGCCGTG ATTGCAGGAG CCCTGGCCGT GGAATTGATG
    GTATCTGTTT TGCAGCATCC AGAAGGGGGC TATGCCATTG CCAGCAGCAG TGACGATCGG
    ATGAATGAGC CTCCAACCTC TCTTGGGCTT GTGCCTCACC AGATCCGGGG ATTTCTTTCA
    CGGTTTGATA ATGTCCTTCC CGTCAGCCTG GCATTTGACA AATGTACAGC TTGTTCTTCC
    AAAGTTCTTG ATCAATATGA ACGAGAAGGA TTTAACTTCC TAGCCAAGGT GTTTAATTCT
    TCACATTCCT TCTTAGAAGA CTTGACTGGT CTTACATTGC TGCATCAAGA AACCCAAGCT
    GCTGAGATCT GGGACATGAG CGATGATGAG ACCATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016861042.1
    CDS624..1802
    Translation

    Target ORF information:

    RefSeq Version XM_017005553.1
    Organism Homo sapiens(human)
    Definition Homo sapiens autophagy related 7 (ATG7), transcript variant X27, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017005553.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGGGACCAA GGATGGTGAA CCTCAGTGAA TGTATGGACC CTAAAAGGTT AGCTGAGTCA 
    TCAGTGGATC TAAATCTCAA ACTGATGTGT TGGAGATTGG TTCCTACTTT AGACTTGGAC
    AAGGTTGTGT CTGTCAAATG TCTGCTGCTT GGAGCCGGCA CCTTGGGTTG CAATGTAGCT
    AGGACGTTGA TGGGTTGGGG CGTGAGACAC ATCACATTTG TGGACAATGC CAAGATCTCC
    TACTCCAATC CTGTGAGGCA GCCTCTCTAT GAGTTTGAAG ATTGCCTAGG GGGTGGTAAG
    CCCAAGGCTC TGGCAGCAGC GGACCGGCTC CAGAAAATAT TCCCCGGTGT GAATGCCAGA
    GGATTCAACA TGAGCATACC TATGCCTGGG CATCCAGTGA ACTTCTCCAG TGTCACTCTG
    GAGCAAGCCC GCAGAGATGT GGAGCAACTG GAGCAGCTCA TCGAAAGCCA TGATGTCGTC
    TTCCTATTGA TGGACACCAG GGAGAGCCGG TGGCTTCCTG CCGTCATTGC TGCAAGCAAG
    AGAAAGCTGG TCATCAATGC TGCTTTGGGA TTTGACACAT TTGTTGTCAT GAGACATGGT
    CTGAAGAAAC CAAAGCAGCA AGGAGCTGGG GACTTGTGTC CAAACCACCC TGTGGCATCT
    GCTGACCTCC TGGGCTCATC GCTTTTTGCC AACATCCCTG GTTACAAGCT TGGCTGCTAC
    TTCTGCAATG ATGTGGTGGC CCCAGGAGAT TCAACCAGAG ACCGGACCTT GGACCAGCAG
    TGCACTGTGA GTCGTCCAGG ACTGGCCGTG ATTGCAGGAG CCCTGGCCGT GGAATTGATG
    GTATCTGTTT TGCAGCATCC AGAAGGGGGC TATGCCATTG CCAGCAGCAG TGACGATCGG
    ATGAATGAGC CTCCAACCTC TCTTGGGCTT GTGCCTCACC AGATCCGGGG ATTTCTTTCA
    CGGTTTGATA ATGTCCTTCC CGTCAGCCTG GCATTTGACA AATGTACAGC TTGTTCTTCC
    AAAGTTCTTG ATCAATATGA ACGAGAAGGA TTTAACTTCC TAGCCAAGGT GTTTAATTCT
    TCACATTCCT TCTTAGAAGA CTTGACTGGT CTTACATTGC TGCATCAAGA AACCCAAGCT
    GCTGAGATCT GGGACATGAG CGATGATGAG ACCATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu93737
    Clone ID Related Accession (Same CDS sequence) XM_017005553.1 , XM_024453315.1
    Accession Version XM_024453315.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1179bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product ubiquitin-like modifier-activating enzyme ATG7 isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGGGACCAA GGATGGTGAA CCTCAGTGAA TGTATGGACC CTAAAAGGTT AGCTGAGTCA 
    TCAGTGGATC TAAATCTCAA ACTGATGTGT TGGAGATTGG TTCCTACTTT AGACTTGGAC
    AAGGTTGTGT CTGTCAAATG TCTGCTGCTT GGAGCCGGCA CCTTGGGTTG CAATGTAGCT
    AGGACGTTGA TGGGTTGGGG CGTGAGACAC ATCACATTTG TGGACAATGC CAAGATCTCC
    TACTCCAATC CTGTGAGGCA GCCTCTCTAT GAGTTTGAAG ATTGCCTAGG GGGTGGTAAG
    CCCAAGGCTC TGGCAGCAGC GGACCGGCTC CAGAAAATAT TCCCCGGTGT GAATGCCAGA
    GGATTCAACA TGAGCATACC TATGCCTGGG CATCCAGTGA ACTTCTCCAG TGTCACTCTG
    GAGCAAGCCC GCAGAGATGT GGAGCAACTG GAGCAGCTCA TCGAAAGCCA TGATGTCGTC
    TTCCTATTGA TGGACACCAG GGAGAGCCGG TGGCTTCCTG CCGTCATTGC TGCAAGCAAG
    AGAAAGCTGG TCATCAATGC TGCTTTGGGA TTTGACACAT TTGTTGTCAT GAGACATGGT
    CTGAAGAAAC CAAAGCAGCA AGGAGCTGGG GACTTGTGTC CAAACCACCC TGTGGCATCT
    GCTGACCTCC TGGGCTCATC GCTTTTTGCC AACATCCCTG GTTACAAGCT TGGCTGCTAC
    TTCTGCAATG ATGTGGTGGC CCCAGGAGAT TCAACCAGAG ACCGGACCTT GGACCAGCAG
    TGCACTGTGA GTCGTCCAGG ACTGGCCGTG ATTGCAGGAG CCCTGGCCGT GGAATTGATG
    GTATCTGTTT TGCAGCATCC AGAAGGGGGC TATGCCATTG CCAGCAGCAG TGACGATCGG
    ATGAATGAGC CTCCAACCTC TCTTGGGCTT GTGCCTCACC AGATCCGGGG ATTTCTTTCA
    CGGTTTGATA ATGTCCTTCC CGTCAGCCTG GCATTTGACA AATGTACAGC TTGTTCTTCC
    AAAGTTCTTG ATCAATATGA ACGAGAAGGA TTTAACTTCC TAGCCAAGGT GTTTAATTCT
    TCACATTCCT TCTTAGAAGA CTTGACTGGT CTTACATTGC TGCATCAAGA AACCCAAGCT
    GCTGAGATCT GGGACATGAG CGATGATGAG ACCATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024309083.1
    CDS850..2028
    Translation

    Target ORF information:

    RefSeq Version XM_024453315.1
    Organism Homo sapiens(human)
    Definition Homo sapiens autophagy related 7 (ATG7), transcript variant X25, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024453315.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGGGACCAA GGATGGTGAA CCTCAGTGAA TGTATGGACC CTAAAAGGTT AGCTGAGTCA 
    TCAGTGGATC TAAATCTCAA ACTGATGTGT TGGAGATTGG TTCCTACTTT AGACTTGGAC
    AAGGTTGTGT CTGTCAAATG TCTGCTGCTT GGAGCCGGCA CCTTGGGTTG CAATGTAGCT
    AGGACGTTGA TGGGTTGGGG CGTGAGACAC ATCACATTTG TGGACAATGC CAAGATCTCC
    TACTCCAATC CTGTGAGGCA GCCTCTCTAT GAGTTTGAAG ATTGCCTAGG GGGTGGTAAG
    CCCAAGGCTC TGGCAGCAGC GGACCGGCTC CAGAAAATAT TCCCCGGTGT GAATGCCAGA
    GGATTCAACA TGAGCATACC TATGCCTGGG CATCCAGTGA ACTTCTCCAG TGTCACTCTG
    GAGCAAGCCC GCAGAGATGT GGAGCAACTG GAGCAGCTCA TCGAAAGCCA TGATGTCGTC
    TTCCTATTGA TGGACACCAG GGAGAGCCGG TGGCTTCCTG CCGTCATTGC TGCAAGCAAG
    AGAAAGCTGG TCATCAATGC TGCTTTGGGA TTTGACACAT TTGTTGTCAT GAGACATGGT
    CTGAAGAAAC CAAAGCAGCA AGGAGCTGGG GACTTGTGTC CAAACCACCC TGTGGCATCT
    GCTGACCTCC TGGGCTCATC GCTTTTTGCC AACATCCCTG GTTACAAGCT TGGCTGCTAC
    TTCTGCAATG ATGTGGTGGC CCCAGGAGAT TCAACCAGAG ACCGGACCTT GGACCAGCAG
    TGCACTGTGA GTCGTCCAGG ACTGGCCGTG ATTGCAGGAG CCCTGGCCGT GGAATTGATG
    GTATCTGTTT TGCAGCATCC AGAAGGGGGC TATGCCATTG CCAGCAGCAG TGACGATCGG
    ATGAATGAGC CTCCAACCTC TCTTGGGCTT GTGCCTCACC AGATCCGGGG ATTTCTTTCA
    CGGTTTGATA ATGTCCTTCC CGTCAGCCTG GCATTTGACA AATGTACAGC TTGTTCTTCC
    AAAGTTCTTG ATCAATATGA ACGAGAAGGA TTTAACTTCC TAGCCAAGGT GTTTAATTCT
    TCACATTCCT TCTTAGAAGA CTTGACTGGT CTTACATTGC TGCATCAAGA AACCCAAGCT
    GCTGAGATCT GGGACATGAG CGATGATGAG ACCATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu25185
    Clone ID Related Accession (Same CDS sequence) NM_001349234.1 , NM_001349235.1 , NM_006395.2 , NM_001349233.1 , NM_001349232.1 , NM_001349232.2 , NM_001349233.2 , NM_001349235.2 , NM_001349234.2 , NM_006395.3
    Accession Version NM_001349234.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2112bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-24
    Organism Homo sapiens(human)
    Product ubiquitin-like modifier-activating enzyme ATG7 isoform a
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC083855.3, AC020750.4, AC026185.4 and AC022001.5. On Mar 9, 2017 this sequence version replaced XM_017005545.1. Transcript Variant: This variant (6), as well as variants 1, 4, 5, and 7, encodes the longest isoform (a). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.32467.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01820689, SAMN01820691 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGCGGCAG CTACGGGGGA TCCTGGACTC TCTAAACTGC AGTTTGCCCC TTTTAGTAGT 
    GCCTTGGATG TTGGGTTTTG GCATGAGTTG ACCCAGAAGA AGCTGAACGA GTATCGGCTG
    GATGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAATG GTGACTCTGC TGGGCTGCCA
    GCTCGCTTAA CATTGGAGTT CAGTGCTTTT GACATGAGTG CTCCCACCCC AGCCCGTTGC
    TGCCCAGCTA TTGGAACACT GTATAACACC AACACACTCG AGTCTTTCAA GACTGCAGAT
    AAGAAGCTCC TTTTGGAACA AGCAGCAAAT GAGATATGGG AATCCATAAA ATCAGGCACT
    GCTCTTGAAA ACCCTGTACT CCTCAACAAG TTCCTCCTCT TGACATTTGC AGATCTAAAG
    AAGTACCACT TCTACTATTG GTTTTGCTAT CCTGCCCTCT GTCTTCCAGA GAGTTTACCT
    CTCATTCAGG GGCCAGTGGG TTTGGATCAA AGGTTTTCAC TAAAACAGAT TGAAGCACTA
    GAGTGTGCAT ATGATAATCT TTGTCAAACA GAAGGAGTCA CAGCTCTTCC TTACTTCTTA
    ATCAAGTATG ATGAGAACAT GGTGCTGGTT TCCTTGCTTA AACACTACAG TGATTTCTTC
    CAAGGTCAAA GGACGAAGAT AACAATTGGT GTATATGATC CCTGTAACTT AGCCCAGTAC
    CCTGGATGGC CTTTGAGGAA TTTTTTGGTC CTAGCAGCCC ACAGATGGAG TAGCAGTTTC
    CAGTCTGTTG AAGTTGTTTG CTTCCGTGAC CGTACCATGC AGGGGGCGAG AGACGTTGCC
    CACAGCATCA TCTTCGAAGT GAAGCTTCCA GAAATGGCAT TTAGCCCAGA TTGTCCTAAA
    GCAGTTGGAT GGGAAAAGAA CCAGAAAGGA GGCATGGGAC CAAGGATGGT GAACCTCAGT
    GAATGTATGG ACCCTAAAAG GTTAGCTGAG TCATCAGTGG ATCTAAATCT CAAACTGATG
    TGTTGGAGAT TGGTTCCTAC TTTAGACTTG GACAAGGTTG TGTCTGTCAA ATGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGCAATGTA GCTAGGACGT TGATGGGTTG GGGCGTGAGA
    CACATCACAT TTGTGGACAA TGCCAAGATC TCCTACTCCA ATCCTGTGAG GCAGCCTCTC
    TATGAGTTTG AAGATTGCCT AGGGGGTGGT AAGCCCAAGG CTCTGGCAGC AGCGGACCGG
    CTCCAGAAAA TATTCCCCGG TGTGAATGCC AGAGGATTCA ACATGAGCAT ACCTATGCCT
    GGGCATCCAG TGAACTTCTC CAGTGTCACT CTGGAGCAAG CCCGCAGAGA TGTGGAGCAA
    CTGGAGCAGC TCATCGAAAG CCATGATGTC GTCTTCCTAT TGATGGACAC CAGGGAGAGC
    CGGTGGCTTC CTGCCGTCAT TGCTGCAAGC AAGAGAAAGC TGGTCATCAA TGCTGCTTTG
    GGATTTGACA CATTTGTTGT CATGAGACAT GGTCTGAAGA AACCAAAGCA GCAAGGAGCT
    GGGGACTTGT GTCCAAACCA CCCTGTGGCA TCTGCTGACC TCCTGGGCTC ATCGCTTTTT
    GCCAACATCC CTGGTTACAA GCTTGGCTGC TACTTCTGCA ATGATGTGGT GGCCCCAGGA
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGTCGTCC AGGACTGGCC
    GTGATTGCAG GAGCCCTGGC CGTGGAATTG ATGGTATCTG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAT CGGATGAATG AGCCTCCAAC CTCTCTTGGG
    CTTGTGCCTC ACCAGATCCG GGGATTTCTT TCACGGTTTG ATAATGTCCT TCCCGTCAGC
    CTGGCATTTG ACAAATGTAC AGCTTGTTCT TCCAAAGTTC TTGATCAATA TGAACGAGAA
    GGATTTAACT TCCTAGCCAA GGTGTTTAAT TCTTCACATT CCTTCTTAGA AGACTTGACT
    GGTCTTACAT TGCTGCATCA AGAAACCCAA GCTGCTGAGA TCTGGGACAT GAGCGATGAT
    GAGACCATCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001336163.1
    CDS213..2324
    Translation

    Target ORF information:

    RefSeq Version NM_001349234.1
    Organism Homo sapiens(human)
    Definition Homo sapiens autophagy related 7 (ATG7), transcript variant 6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001349234.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGCGGCAG CTACGGGGGA TCCTGGACTC TCTAAACTGC AGTTTGCCCC TTTTAGTAGT 
    GCCTTGGATG TTGGGTTTTG GCATGAGTTG ACCCAGAAGA AGCTGAACGA GTATCGGCTG
    GATGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAATG GTGACTCTGC TGGGCTGCCA
    GCTCGCTTAA CATTGGAGTT CAGTGCTTTT GACATGAGTG CTCCCACCCC AGCCCGTTGC
    TGCCCAGCTA TTGGAACACT GTATAACACC AACACACTCG AGTCTTTCAA GACTGCAGAT
    AAGAAGCTCC TTTTGGAACA AGCAGCAAAT GAGATATGGG AATCCATAAA ATCAGGCACT
    GCTCTTGAAA ACCCTGTACT CCTCAACAAG TTCCTCCTCT TGACATTTGC AGATCTAAAG
    AAGTACCACT TCTACTATTG GTTTTGCTAT CCTGCCCTCT GTCTTCCAGA GAGTTTACCT
    CTCATTCAGG GGCCAGTGGG TTTGGATCAA AGGTTTTCAC TAAAACAGAT TGAAGCACTA
    GAGTGTGCAT ATGATAATCT TTGTCAAACA GAAGGAGTCA CAGCTCTTCC TTACTTCTTA
    ATCAAGTATG ATGAGAACAT GGTGCTGGTT TCCTTGCTTA AACACTACAG TGATTTCTTC
    CAAGGTCAAA GGACGAAGAT AACAATTGGT GTATATGATC CCTGTAACTT AGCCCAGTAC
    CCTGGATGGC CTTTGAGGAA TTTTTTGGTC CTAGCAGCCC ACAGATGGAG TAGCAGTTTC
    CAGTCTGTTG AAGTTGTTTG CTTCCGTGAC CGTACCATGC AGGGGGCGAG AGACGTTGCC
    CACAGCATCA TCTTCGAAGT GAAGCTTCCA GAAATGGCAT TTAGCCCAGA TTGTCCTAAA
    GCAGTTGGAT GGGAAAAGAA CCAGAAAGGA GGCATGGGAC CAAGGATGGT GAACCTCAGT
    GAATGTATGG ACCCTAAAAG GTTAGCTGAG TCATCAGTGG ATCTAAATCT CAAACTGATG
    TGTTGGAGAT TGGTTCCTAC TTTAGACTTG GACAAGGTTG TGTCTGTCAA ATGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGCAATGTA GCTAGGACGT TGATGGGTTG GGGCGTGAGA
    CACATCACAT TTGTGGACAA TGCCAAGATC TCCTACTCCA ATCCTGTGAG GCAGCCTCTC
    TATGAGTTTG AAGATTGCCT AGGGGGTGGT AAGCCCAAGG CTCTGGCAGC AGCGGACCGG
    CTCCAGAAAA TATTCCCCGG TGTGAATGCC AGAGGATTCA ACATGAGCAT ACCTATGCCT
    GGGCATCCAG TGAACTTCTC CAGTGTCACT CTGGAGCAAG CCCGCAGAGA TGTGGAGCAA
    CTGGAGCAGC TCATCGAAAG CCATGATGTC GTCTTCCTAT TGATGGACAC CAGGGAGAGC
    CGGTGGCTTC CTGCCGTCAT TGCTGCAAGC AAGAGAAAGC TGGTCATCAA TGCTGCTTTG
    GGATTTGACA CATTTGTTGT CATGAGACAT GGTCTGAAGA AACCAAAGCA GCAAGGAGCT
    GGGGACTTGT GTCCAAACCA CCCTGTGGCA TCTGCTGACC TCCTGGGCTC ATCGCTTTTT
    GCCAACATCC CTGGTTACAA GCTTGGCTGC TACTTCTGCA ATGATGTGGT GGCCCCAGGA
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGTCGTCC AGGACTGGCC
    GTGATTGCAG GAGCCCTGGC CGTGGAATTG ATGGTATCTG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAT CGGATGAATG AGCCTCCAAC CTCTCTTGGG
    CTTGTGCCTC ACCAGATCCG GGGATTTCTT TCACGGTTTG ATAATGTCCT TCCCGTCAGC
    CTGGCATTTG ACAAATGTAC AGCTTGTTCT TCCAAAGTTC TTGATCAATA TGAACGAGAA
    GGATTTAACT TCCTAGCCAA GGTGTTTAAT TCTTCACATT CCTTCTTAGA AGACTTGACT
    GGTCTTACAT TGCTGCATCA AGAAACCCAA GCTGCTGAGA TCTGGGACAT GAGCGATGAT
    GAGACCATCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu25185
    Clone ID Related Accession (Same CDS sequence) NM_001349234.1 , NM_001349235.1 , NM_006395.2 , NM_001349233.1 , NM_001349232.1 , NM_001349232.2 , NM_001349233.2 , NM_001349235.2 , NM_001349234.2 , NM_006395.3
    Accession Version NM_001349235.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2112bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-25
    Organism Homo sapiens(human)
    Product ubiquitin-like modifier-activating enzyme ATG7 isoform a
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC083855.3, AC020750.4, AC026185.4 and AC022001.5. On Mar 9, 2017 this sequence version replaced XM_017005544.1. Transcript Variant: This variant (7), as well as variants 1, 4, 5, and 6, encodes the longest isoform (a). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.272133.1, SRR1660805.131804.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGCGGCAG CTACGGGGGA TCCTGGACTC TCTAAACTGC AGTTTGCCCC TTTTAGTAGT 
    GCCTTGGATG TTGGGTTTTG GCATGAGTTG ACCCAGAAGA AGCTGAACGA GTATCGGCTG
    GATGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAATG GTGACTCTGC TGGGCTGCCA
    GCTCGCTTAA CATTGGAGTT CAGTGCTTTT GACATGAGTG CTCCCACCCC AGCCCGTTGC
    TGCCCAGCTA TTGGAACACT GTATAACACC AACACACTCG AGTCTTTCAA GACTGCAGAT
    AAGAAGCTCC TTTTGGAACA AGCAGCAAAT GAGATATGGG AATCCATAAA ATCAGGCACT
    GCTCTTGAAA ACCCTGTACT CCTCAACAAG TTCCTCCTCT TGACATTTGC AGATCTAAAG
    AAGTACCACT TCTACTATTG GTTTTGCTAT CCTGCCCTCT GTCTTCCAGA GAGTTTACCT
    CTCATTCAGG GGCCAGTGGG TTTGGATCAA AGGTTTTCAC TAAAACAGAT TGAAGCACTA
    GAGTGTGCAT ATGATAATCT TTGTCAAACA GAAGGAGTCA CAGCTCTTCC TTACTTCTTA
    ATCAAGTATG ATGAGAACAT GGTGCTGGTT TCCTTGCTTA AACACTACAG TGATTTCTTC
    CAAGGTCAAA GGACGAAGAT AACAATTGGT GTATATGATC CCTGTAACTT AGCCCAGTAC
    CCTGGATGGC CTTTGAGGAA TTTTTTGGTC CTAGCAGCCC ACAGATGGAG TAGCAGTTTC
    CAGTCTGTTG AAGTTGTTTG CTTCCGTGAC CGTACCATGC AGGGGGCGAG AGACGTTGCC
    CACAGCATCA TCTTCGAAGT GAAGCTTCCA GAAATGGCAT TTAGCCCAGA TTGTCCTAAA
    GCAGTTGGAT GGGAAAAGAA CCAGAAAGGA GGCATGGGAC CAAGGATGGT GAACCTCAGT
    GAATGTATGG ACCCTAAAAG GTTAGCTGAG TCATCAGTGG ATCTAAATCT CAAACTGATG
    TGTTGGAGAT TGGTTCCTAC TTTAGACTTG GACAAGGTTG TGTCTGTCAA ATGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGCAATGTA GCTAGGACGT TGATGGGTTG GGGCGTGAGA
    CACATCACAT TTGTGGACAA TGCCAAGATC TCCTACTCCA ATCCTGTGAG GCAGCCTCTC
    TATGAGTTTG AAGATTGCCT AGGGGGTGGT AAGCCCAAGG CTCTGGCAGC AGCGGACCGG
    CTCCAGAAAA TATTCCCCGG TGTGAATGCC AGAGGATTCA ACATGAGCAT ACCTATGCCT
    GGGCATCCAG TGAACTTCTC CAGTGTCACT CTGGAGCAAG CCCGCAGAGA TGTGGAGCAA
    CTGGAGCAGC TCATCGAAAG CCATGATGTC GTCTTCCTAT TGATGGACAC CAGGGAGAGC
    CGGTGGCTTC CTGCCGTCAT TGCTGCAAGC AAGAGAAAGC TGGTCATCAA TGCTGCTTTG
    GGATTTGACA CATTTGTTGT CATGAGACAT GGTCTGAAGA AACCAAAGCA GCAAGGAGCT
    GGGGACTTGT GTCCAAACCA CCCTGTGGCA TCTGCTGACC TCCTGGGCTC ATCGCTTTTT
    GCCAACATCC CTGGTTACAA GCTTGGCTGC TACTTCTGCA ATGATGTGGT GGCCCCAGGA
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGTCGTCC AGGACTGGCC
    GTGATTGCAG GAGCCCTGGC CGTGGAATTG ATGGTATCTG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAT CGGATGAATG AGCCTCCAAC CTCTCTTGGG
    CTTGTGCCTC ACCAGATCCG GGGATTTCTT TCACGGTTTG ATAATGTCCT TCCCGTCAGC
    CTGGCATTTG ACAAATGTAC AGCTTGTTCT TCCAAAGTTC TTGATCAATA TGAACGAGAA
    GGATTTAACT TCCTAGCCAA GGTGTTTAAT TCTTCACATT CCTTCTTAGA AGACTTGACT
    GGTCTTACAT TGCTGCATCA AGAAACCCAA GCTGCTGAGA TCTGGGACAT GAGCGATGAT
    GAGACCATCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001336164.1
    CDS364..2475
    Translation

    Target ORF information:

    RefSeq Version NM_001349235.1
    Organism Homo sapiens(human)
    Definition Homo sapiens autophagy related 7 (ATG7), transcript variant 7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001349235.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGCGGCAG CTACGGGGGA TCCTGGACTC TCTAAACTGC AGTTTGCCCC TTTTAGTAGT 
    GCCTTGGATG TTGGGTTTTG GCATGAGTTG ACCCAGAAGA AGCTGAACGA GTATCGGCTG
    GATGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAATG GTGACTCTGC TGGGCTGCCA
    GCTCGCTTAA CATTGGAGTT CAGTGCTTTT GACATGAGTG CTCCCACCCC AGCCCGTTGC
    TGCCCAGCTA TTGGAACACT GTATAACACC AACACACTCG AGTCTTTCAA GACTGCAGAT
    AAGAAGCTCC TTTTGGAACA AGCAGCAAAT GAGATATGGG AATCCATAAA ATCAGGCACT
    GCTCTTGAAA ACCCTGTACT CCTCAACAAG TTCCTCCTCT TGACATTTGC AGATCTAAAG
    AAGTACCACT TCTACTATTG GTTTTGCTAT CCTGCCCTCT GTCTTCCAGA GAGTTTACCT
    CTCATTCAGG GGCCAGTGGG TTTGGATCAA AGGTTTTCAC TAAAACAGAT TGAAGCACTA
    GAGTGTGCAT ATGATAATCT TTGTCAAACA GAAGGAGTCA CAGCTCTTCC TTACTTCTTA
    ATCAAGTATG ATGAGAACAT GGTGCTGGTT TCCTTGCTTA AACACTACAG TGATTTCTTC
    CAAGGTCAAA GGACGAAGAT AACAATTGGT GTATATGATC CCTGTAACTT AGCCCAGTAC
    CCTGGATGGC CTTTGAGGAA TTTTTTGGTC CTAGCAGCCC ACAGATGGAG TAGCAGTTTC
    CAGTCTGTTG AAGTTGTTTG CTTCCGTGAC CGTACCATGC AGGGGGCGAG AGACGTTGCC
    CACAGCATCA TCTTCGAAGT GAAGCTTCCA GAAATGGCAT TTAGCCCAGA TTGTCCTAAA
    GCAGTTGGAT GGGAAAAGAA CCAGAAAGGA GGCATGGGAC CAAGGATGGT GAACCTCAGT
    GAATGTATGG ACCCTAAAAG GTTAGCTGAG TCATCAGTGG ATCTAAATCT CAAACTGATG
    TGTTGGAGAT TGGTTCCTAC TTTAGACTTG GACAAGGTTG TGTCTGTCAA ATGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGCAATGTA GCTAGGACGT TGATGGGTTG GGGCGTGAGA
    CACATCACAT TTGTGGACAA TGCCAAGATC TCCTACTCCA ATCCTGTGAG GCAGCCTCTC
    TATGAGTTTG AAGATTGCCT AGGGGGTGGT AAGCCCAAGG CTCTGGCAGC AGCGGACCGG
    CTCCAGAAAA TATTCCCCGG TGTGAATGCC AGAGGATTCA ACATGAGCAT ACCTATGCCT
    GGGCATCCAG TGAACTTCTC CAGTGTCACT CTGGAGCAAG CCCGCAGAGA TGTGGAGCAA
    CTGGAGCAGC TCATCGAAAG CCATGATGTC GTCTTCCTAT TGATGGACAC CAGGGAGAGC
    CGGTGGCTTC CTGCCGTCAT TGCTGCAAGC AAGAGAAAGC TGGTCATCAA TGCTGCTTTG
    GGATTTGACA CATTTGTTGT CATGAGACAT GGTCTGAAGA AACCAAAGCA GCAAGGAGCT
    GGGGACTTGT GTCCAAACCA CCCTGTGGCA TCTGCTGACC TCCTGGGCTC ATCGCTTTTT
    GCCAACATCC CTGGTTACAA GCTTGGCTGC TACTTCTGCA ATGATGTGGT GGCCCCAGGA
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGTCGTCC AGGACTGGCC
    GTGATTGCAG GAGCCCTGGC CGTGGAATTG ATGGTATCTG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAT CGGATGAATG AGCCTCCAAC CTCTCTTGGG
    CTTGTGCCTC ACCAGATCCG GGGATTTCTT TCACGGTTTG ATAATGTCCT TCCCGTCAGC
    CTGGCATTTG ACAAATGTAC AGCTTGTTCT TCCAAAGTTC TTGATCAATA TGAACGAGAA
    GGATTTAACT TCCTAGCCAA GGTGTTTAAT TCTTCACATT CCTTCTTAGA AGACTTGACT
    GGTCTTACAT TGCTGCATCA AGAAACCCAA GCTGCTGAGA TCTGGGACAT GAGCGATGAT
    GAGACCATCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu25185
    Clone ID Related Accession (Same CDS sequence) NM_001349234.1 , NM_001349235.1 , NM_006395.2 , NM_001349233.1 , NM_001349232.1 , NM_001349232.2 , NM_001349233.2 , NM_001349235.2 , NM_001349234.2 , NM_006395.3
    Accession Version NM_006395.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2112bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-19
    Organism Homo sapiens(human)
    Product ubiquitin-like modifier-activating enzyme ATG7 isoform a
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA177126.1, AC083855.3, BC000091.2, AK075221.1, AL122075.1 and AC022001.5. On Jan 30, 2009 this sequence version replaced NM_006395.1. Transcript Variant: This variant (1), as well as variants 4, 5, 6, and 7, encodes the longest isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.36109.1, SRR1660805.158295.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGCGGCAG CTACGGGGGA TCCTGGACTC TCTAAACTGC AGTTTGCCCC TTTTAGTAGT 
    GCCTTGGATG TTGGGTTTTG GCATGAGTTG ACCCAGAAGA AGCTGAACGA GTATCGGCTG
    GATGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAATG GTGACTCTGC TGGGCTGCCA
    GCTCGCTTAA CATTGGAGTT CAGTGCTTTT GACATGAGTG CTCCCACCCC AGCCCGTTGC
    TGCCCAGCTA TTGGAACACT GTATAACACC AACACACTCG AGTCTTTCAA GACTGCAGAT
    AAGAAGCTCC TTTTGGAACA AGCAGCAAAT GAGATATGGG AATCCATAAA ATCAGGCACT
    GCTCTTGAAA ACCCTGTACT CCTCAACAAG TTCCTCCTCT TGACATTTGC AGATCTAAAG
    AAGTACCACT TCTACTATTG GTTTTGCTAT CCTGCCCTCT GTCTTCCAGA GAGTTTACCT
    CTCATTCAGG GGCCAGTGGG TTTGGATCAA AGGTTTTCAC TAAAACAGAT TGAAGCACTA
    GAGTGTGCAT ATGATAATCT TTGTCAAACA GAAGGAGTCA CAGCTCTTCC TTACTTCTTA
    ATCAAGTATG ATGAGAACAT GGTGCTGGTT TCCTTGCTTA AACACTACAG TGATTTCTTC
    CAAGGTCAAA GGACGAAGAT AACAATTGGT GTATATGATC CCTGTAACTT AGCCCAGTAC
    CCTGGATGGC CTTTGAGGAA TTTTTTGGTC CTAGCAGCCC ACAGATGGAG TAGCAGTTTC
    CAGTCTGTTG AAGTTGTTTG CTTCCGTGAC CGTACCATGC AGGGGGCGAG AGACGTTGCC
    CACAGCATCA TCTTCGAAGT GAAGCTTCCA GAAATGGCAT TTAGCCCAGA TTGTCCTAAA
    GCAGTTGGAT GGGAAAAGAA CCAGAAAGGA GGCATGGGAC CAAGGATGGT GAACCTCAGT
    GAATGTATGG ACCCTAAAAG GTTAGCTGAG TCATCAGTGG ATCTAAATCT CAAACTGATG
    TGTTGGAGAT TGGTTCCTAC TTTAGACTTG GACAAGGTTG TGTCTGTCAA ATGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGCAATGTA GCTAGGACGT TGATGGGTTG GGGCGTGAGA
    CACATCACAT TTGTGGACAA TGCCAAGATC TCCTACTCCA ATCCTGTGAG GCAGCCTCTC
    TATGAGTTTG AAGATTGCCT AGGGGGTGGT AAGCCCAAGG CTCTGGCAGC AGCGGACCGG
    CTCCAGAAAA TATTCCCCGG TGTGAATGCC AGAGGATTCA ACATGAGCAT ACCTATGCCT
    GGGCATCCAG TGAACTTCTC CAGTGTCACT CTGGAGCAAG CCCGCAGAGA TGTGGAGCAA
    CTGGAGCAGC TCATCGAAAG CCATGATGTC GTCTTCCTAT TGATGGACAC CAGGGAGAGC
    CGGTGGCTTC CTGCCGTCAT TGCTGCAAGC AAGAGAAAGC TGGTCATCAA TGCTGCTTTG
    GGATTTGACA CATTTGTTGT CATGAGACAT GGTCTGAAGA AACCAAAGCA GCAAGGAGCT
    GGGGACTTGT GTCCAAACCA CCCTGTGGCA TCTGCTGACC TCCTGGGCTC ATCGCTTTTT
    GCCAACATCC CTGGTTACAA GCTTGGCTGC TACTTCTGCA ATGATGTGGT GGCCCCAGGA
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGTCGTCC AGGACTGGCC
    GTGATTGCAG GAGCCCTGGC CGTGGAATTG ATGGTATCTG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAT CGGATGAATG AGCCTCCAAC CTCTCTTGGG
    CTTGTGCCTC ACCAGATCCG GGGATTTCTT TCACGGTTTG ATAATGTCCT TCCCGTCAGC
    CTGGCATTTG ACAAATGTAC AGCTTGTTCT TCCAAAGTTC TTGATCAATA TGAACGAGAA
    GGATTTAACT TCCTAGCCAA GGTGTTTAAT TCTTCACATT CCTTCTTAGA AGACTTGACT
    GGTCTTACAT TGCTGCATCA AGAAACCCAA GCTGCTGAGA TCTGGGACAT GAGCGATGAT
    GAGACCATCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_006386.1
    CDS118..2229
    Translation

    Target ORF information:

    RefSeq Version NM_006395.2
    Organism Homo sapiens(human)
    Definition Homo sapiens autophagy related 7 (ATG7), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_006395.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGCGGCAG CTACGGGGGA TCCTGGACTC TCTAAACTGC AGTTTGCCCC TTTTAGTAGT 
    GCCTTGGATG TTGGGTTTTG GCATGAGTTG ACCCAGAAGA AGCTGAACGA GTATCGGCTG
    GATGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAATG GTGACTCTGC TGGGCTGCCA
    GCTCGCTTAA CATTGGAGTT CAGTGCTTTT GACATGAGTG CTCCCACCCC AGCCCGTTGC
    TGCCCAGCTA TTGGAACACT GTATAACACC AACACACTCG AGTCTTTCAA GACTGCAGAT
    AAGAAGCTCC TTTTGGAACA AGCAGCAAAT GAGATATGGG AATCCATAAA ATCAGGCACT
    GCTCTTGAAA ACCCTGTACT CCTCAACAAG TTCCTCCTCT TGACATTTGC AGATCTAAAG
    AAGTACCACT TCTACTATTG GTTTTGCTAT CCTGCCCTCT GTCTTCCAGA GAGTTTACCT
    CTCATTCAGG GGCCAGTGGG TTTGGATCAA AGGTTTTCAC TAAAACAGAT TGAAGCACTA
    GAGTGTGCAT ATGATAATCT TTGTCAAACA GAAGGAGTCA CAGCTCTTCC TTACTTCTTA
    ATCAAGTATG ATGAGAACAT GGTGCTGGTT TCCTTGCTTA AACACTACAG TGATTTCTTC
    CAAGGTCAAA GGACGAAGAT AACAATTGGT GTATATGATC CCTGTAACTT AGCCCAGTAC
    CCTGGATGGC CTTTGAGGAA TTTTTTGGTC CTAGCAGCCC ACAGATGGAG TAGCAGTTTC
    CAGTCTGTTG AAGTTGTTTG CTTCCGTGAC CGTACCATGC AGGGGGCGAG AGACGTTGCC
    CACAGCATCA TCTTCGAAGT GAAGCTTCCA GAAATGGCAT TTAGCCCAGA TTGTCCTAAA
    GCAGTTGGAT GGGAAAAGAA CCAGAAAGGA GGCATGGGAC CAAGGATGGT GAACCTCAGT
    GAATGTATGG ACCCTAAAAG GTTAGCTGAG TCATCAGTGG ATCTAAATCT CAAACTGATG
    TGTTGGAGAT TGGTTCCTAC TTTAGACTTG GACAAGGTTG TGTCTGTCAA ATGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGCAATGTA GCTAGGACGT TGATGGGTTG GGGCGTGAGA
    CACATCACAT TTGTGGACAA TGCCAAGATC TCCTACTCCA ATCCTGTGAG GCAGCCTCTC
    TATGAGTTTG AAGATTGCCT AGGGGGTGGT AAGCCCAAGG CTCTGGCAGC AGCGGACCGG
    CTCCAGAAAA TATTCCCCGG TGTGAATGCC AGAGGATTCA ACATGAGCAT ACCTATGCCT
    GGGCATCCAG TGAACTTCTC CAGTGTCACT CTGGAGCAAG CCCGCAGAGA TGTGGAGCAA
    CTGGAGCAGC TCATCGAAAG CCATGATGTC GTCTTCCTAT TGATGGACAC CAGGGAGAGC
    CGGTGGCTTC CTGCCGTCAT TGCTGCAAGC AAGAGAAAGC TGGTCATCAA TGCTGCTTTG
    GGATTTGACA CATTTGTTGT CATGAGACAT GGTCTGAAGA AACCAAAGCA GCAAGGAGCT
    GGGGACTTGT GTCCAAACCA CCCTGTGGCA TCTGCTGACC TCCTGGGCTC ATCGCTTTTT
    GCCAACATCC CTGGTTACAA GCTTGGCTGC TACTTCTGCA ATGATGTGGT GGCCCCAGGA
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGTCGTCC AGGACTGGCC
    GTGATTGCAG GAGCCCTGGC CGTGGAATTG ATGGTATCTG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAT CGGATGAATG AGCCTCCAAC CTCTCTTGGG
    CTTGTGCCTC ACCAGATCCG GGGATTTCTT TCACGGTTTG ATAATGTCCT TCCCGTCAGC
    CTGGCATTTG ACAAATGTAC AGCTTGTTCT TCCAAAGTTC TTGATCAATA TGAACGAGAA
    GGATTTAACT TCCTAGCCAA GGTGTTTAAT TCTTCACATT CCTTCTTAGA AGACTTGACT
    GGTCTTACAT TGCTGCATCA AGAAACCCAA GCTGCTGAGA TCTGGGACAT GAGCGATGAT
    GAGACCATCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu25185
    Clone ID Related Accession (Same CDS sequence) NM_001349234.1 , NM_001349235.1 , NM_006395.2 , NM_001349233.1 , NM_001349232.1 , NM_001349232.2 , NM_001349233.2 , NM_001349235.2 , NM_001349234.2 , NM_006395.3
    Accession Version NM_001349233.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2112bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-25
    Organism Homo sapiens(human)
    Product ubiquitin-like modifier-activating enzyme ATG7 isoform a
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC083855.3, AC020750.4, AC026185.4 and AC022001.5. On Mar 9, 2017 this sequence version replaced XM_017005546.1. Transcript Variant: This variant (5), as well as variants 1, 4, 6, and 7, encodes the longest isoform (a). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.66302.1, SRR1803614.149195.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGCGGCAG CTACGGGGGA TCCTGGACTC TCTAAACTGC AGTTTGCCCC TTTTAGTAGT 
    GCCTTGGATG TTGGGTTTTG GCATGAGTTG ACCCAGAAGA AGCTGAACGA GTATCGGCTG
    GATGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAATG GTGACTCTGC TGGGCTGCCA
    GCTCGCTTAA CATTGGAGTT CAGTGCTTTT GACATGAGTG CTCCCACCCC AGCCCGTTGC
    TGCCCAGCTA TTGGAACACT GTATAACACC AACACACTCG AGTCTTTCAA GACTGCAGAT
    AAGAAGCTCC TTTTGGAACA AGCAGCAAAT GAGATATGGG AATCCATAAA ATCAGGCACT
    GCTCTTGAAA ACCCTGTACT CCTCAACAAG TTCCTCCTCT TGACATTTGC AGATCTAAAG
    AAGTACCACT TCTACTATTG GTTTTGCTAT CCTGCCCTCT GTCTTCCAGA GAGTTTACCT
    CTCATTCAGG GGCCAGTGGG TTTGGATCAA AGGTTTTCAC TAAAACAGAT TGAAGCACTA
    GAGTGTGCAT ATGATAATCT TTGTCAAACA GAAGGAGTCA CAGCTCTTCC TTACTTCTTA
    ATCAAGTATG ATGAGAACAT GGTGCTGGTT TCCTTGCTTA AACACTACAG TGATTTCTTC
    CAAGGTCAAA GGACGAAGAT AACAATTGGT GTATATGATC CCTGTAACTT AGCCCAGTAC
    CCTGGATGGC CTTTGAGGAA TTTTTTGGTC CTAGCAGCCC ACAGATGGAG TAGCAGTTTC
    CAGTCTGTTG AAGTTGTTTG CTTCCGTGAC CGTACCATGC AGGGGGCGAG AGACGTTGCC
    CACAGCATCA TCTTCGAAGT GAAGCTTCCA GAAATGGCAT TTAGCCCAGA TTGTCCTAAA
    GCAGTTGGAT GGGAAAAGAA CCAGAAAGGA GGCATGGGAC CAAGGATGGT GAACCTCAGT
    GAATGTATGG ACCCTAAAAG GTTAGCTGAG TCATCAGTGG ATCTAAATCT CAAACTGATG
    TGTTGGAGAT TGGTTCCTAC TTTAGACTTG GACAAGGTTG TGTCTGTCAA ATGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGCAATGTA GCTAGGACGT TGATGGGTTG GGGCGTGAGA
    CACATCACAT TTGTGGACAA TGCCAAGATC TCCTACTCCA ATCCTGTGAG GCAGCCTCTC
    TATGAGTTTG AAGATTGCCT AGGGGGTGGT AAGCCCAAGG CTCTGGCAGC AGCGGACCGG
    CTCCAGAAAA TATTCCCCGG TGTGAATGCC AGAGGATTCA ACATGAGCAT ACCTATGCCT
    GGGCATCCAG TGAACTTCTC CAGTGTCACT CTGGAGCAAG CCCGCAGAGA TGTGGAGCAA
    CTGGAGCAGC TCATCGAAAG CCATGATGTC GTCTTCCTAT TGATGGACAC CAGGGAGAGC
    CGGTGGCTTC CTGCCGTCAT TGCTGCAAGC AAGAGAAAGC TGGTCATCAA TGCTGCTTTG
    GGATTTGACA CATTTGTTGT CATGAGACAT GGTCTGAAGA AACCAAAGCA GCAAGGAGCT
    GGGGACTTGT GTCCAAACCA CCCTGTGGCA TCTGCTGACC TCCTGGGCTC ATCGCTTTTT
    GCCAACATCC CTGGTTACAA GCTTGGCTGC TACTTCTGCA ATGATGTGGT GGCCCCAGGA
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGTCGTCC AGGACTGGCC
    GTGATTGCAG GAGCCCTGGC CGTGGAATTG ATGGTATCTG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAT CGGATGAATG AGCCTCCAAC CTCTCTTGGG
    CTTGTGCCTC ACCAGATCCG GGGATTTCTT TCACGGTTTG ATAATGTCCT TCCCGTCAGC
    CTGGCATTTG ACAAATGTAC AGCTTGTTCT TCCAAAGTTC TTGATCAATA TGAACGAGAA
    GGATTTAACT TCCTAGCCAA GGTGTTTAAT TCTTCACATT CCTTCTTAGA AGACTTGACT
    GGTCTTACAT TGCTGCATCA AGAAACCCAA GCTGCTGAGA TCTGGGACAT GAGCGATGAT
    GAGACCATCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001336162.1
    CDS227..2338
    Translation

    Target ORF information:

    RefSeq Version NM_001349233.1
    Organism Homo sapiens(human)
    Definition Homo sapiens autophagy related 7 (ATG7), transcript variant 5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001349233.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGCGGCAG CTACGGGGGA TCCTGGACTC TCTAAACTGC AGTTTGCCCC TTTTAGTAGT 
    GCCTTGGATG TTGGGTTTTG GCATGAGTTG ACCCAGAAGA AGCTGAACGA GTATCGGCTG
    GATGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAATG GTGACTCTGC TGGGCTGCCA
    GCTCGCTTAA CATTGGAGTT CAGTGCTTTT GACATGAGTG CTCCCACCCC AGCCCGTTGC
    TGCCCAGCTA TTGGAACACT GTATAACACC AACACACTCG AGTCTTTCAA GACTGCAGAT
    AAGAAGCTCC TTTTGGAACA AGCAGCAAAT GAGATATGGG AATCCATAAA ATCAGGCACT
    GCTCTTGAAA ACCCTGTACT CCTCAACAAG TTCCTCCTCT TGACATTTGC AGATCTAAAG
    AAGTACCACT TCTACTATTG GTTTTGCTAT CCTGCCCTCT GTCTTCCAGA GAGTTTACCT
    CTCATTCAGG GGCCAGTGGG TTTGGATCAA AGGTTTTCAC TAAAACAGAT TGAAGCACTA
    GAGTGTGCAT ATGATAATCT TTGTCAAACA GAAGGAGTCA CAGCTCTTCC TTACTTCTTA
    ATCAAGTATG ATGAGAACAT GGTGCTGGTT TCCTTGCTTA AACACTACAG TGATTTCTTC
    CAAGGTCAAA GGACGAAGAT AACAATTGGT GTATATGATC CCTGTAACTT AGCCCAGTAC
    CCTGGATGGC CTTTGAGGAA TTTTTTGGTC CTAGCAGCCC ACAGATGGAG TAGCAGTTTC
    CAGTCTGTTG AAGTTGTTTG CTTCCGTGAC CGTACCATGC AGGGGGCGAG AGACGTTGCC
    CACAGCATCA TCTTCGAAGT GAAGCTTCCA GAAATGGCAT TTAGCCCAGA TTGTCCTAAA
    GCAGTTGGAT GGGAAAAGAA CCAGAAAGGA GGCATGGGAC CAAGGATGGT GAACCTCAGT
    GAATGTATGG ACCCTAAAAG GTTAGCTGAG TCATCAGTGG ATCTAAATCT CAAACTGATG
    TGTTGGAGAT TGGTTCCTAC TTTAGACTTG GACAAGGTTG TGTCTGTCAA ATGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGCAATGTA GCTAGGACGT TGATGGGTTG GGGCGTGAGA
    CACATCACAT TTGTGGACAA TGCCAAGATC TCCTACTCCA ATCCTGTGAG GCAGCCTCTC
    TATGAGTTTG AAGATTGCCT AGGGGGTGGT AAGCCCAAGG CTCTGGCAGC AGCGGACCGG
    CTCCAGAAAA TATTCCCCGG TGTGAATGCC AGAGGATTCA ACATGAGCAT ACCTATGCCT
    GGGCATCCAG TGAACTTCTC CAGTGTCACT CTGGAGCAAG CCCGCAGAGA TGTGGAGCAA
    CTGGAGCAGC TCATCGAAAG CCATGATGTC GTCTTCCTAT TGATGGACAC CAGGGAGAGC
    CGGTGGCTTC CTGCCGTCAT TGCTGCAAGC AAGAGAAAGC TGGTCATCAA TGCTGCTTTG
    GGATTTGACA CATTTGTTGT CATGAGACAT GGTCTGAAGA AACCAAAGCA GCAAGGAGCT
    GGGGACTTGT GTCCAAACCA CCCTGTGGCA TCTGCTGACC TCCTGGGCTC ATCGCTTTTT
    GCCAACATCC CTGGTTACAA GCTTGGCTGC TACTTCTGCA ATGATGTGGT GGCCCCAGGA
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGTCGTCC AGGACTGGCC
    GTGATTGCAG GAGCCCTGGC CGTGGAATTG ATGGTATCTG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAT CGGATGAATG AGCCTCCAAC CTCTCTTGGG
    CTTGTGCCTC ACCAGATCCG GGGATTTCTT TCACGGTTTG ATAATGTCCT TCCCGTCAGC
    CTGGCATTTG ACAAATGTAC AGCTTGTTCT TCCAAAGTTC TTGATCAATA TGAACGAGAA
    GGATTTAACT TCCTAGCCAA GGTGTTTAAT TCTTCACATT CCTTCTTAGA AGACTTGACT
    GGTCTTACAT TGCTGCATCA AGAAACCCAA GCTGCTGAGA TCTGGGACAT GAGCGATGAT
    GAGACCATCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu25185
    Clone ID Related Accession (Same CDS sequence) NM_001349234.1 , NM_001349235.1 , NM_006395.2 , NM_001349233.1 , NM_001349232.1 , NM_001349232.2 , NM_001349233.2 , NM_001349235.2 , NM_001349234.2 , NM_006395.3
    Accession Version NM_001349232.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2112bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-25
    Organism Homo sapiens(human)
    Product ubiquitin-like modifier-activating enzyme ATG7 isoform a
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC083855.3, AC020750.4, AC026185.4 and AC022001.5. On Mar 9, 2017 this sequence version replaced XM_011533282.2. Transcript Variant: This variant (4) represents the longest transcript and encodes the longest isoform (a). Variants 1, 4, 5, 6, and 7 all encode the same isoform (a). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.83723.1, SRR1803615.122845.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGCGGCAG CTACGGGGGA TCCTGGACTC TCTAAACTGC AGTTTGCCCC TTTTAGTAGT 
    GCCTTGGATG TTGGGTTTTG GCATGAGTTG ACCCAGAAGA AGCTGAACGA GTATCGGCTG
    GATGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAATG GTGACTCTGC TGGGCTGCCA
    GCTCGCTTAA CATTGGAGTT CAGTGCTTTT GACATGAGTG CTCCCACCCC AGCCCGTTGC
    TGCCCAGCTA TTGGAACACT GTATAACACC AACACACTCG AGTCTTTCAA GACTGCAGAT
    AAGAAGCTCC TTTTGGAACA AGCAGCAAAT GAGATATGGG AATCCATAAA ATCAGGCACT
    GCTCTTGAAA ACCCTGTACT CCTCAACAAG TTCCTCCTCT TGACATTTGC AGATCTAAAG
    AAGTACCACT TCTACTATTG GTTTTGCTAT CCTGCCCTCT GTCTTCCAGA GAGTTTACCT
    CTCATTCAGG GGCCAGTGGG TTTGGATCAA AGGTTTTCAC TAAAACAGAT TGAAGCACTA
    GAGTGTGCAT ATGATAATCT TTGTCAAACA GAAGGAGTCA CAGCTCTTCC TTACTTCTTA
    ATCAAGTATG ATGAGAACAT GGTGCTGGTT TCCTTGCTTA AACACTACAG TGATTTCTTC
    CAAGGTCAAA GGACGAAGAT AACAATTGGT GTATATGATC CCTGTAACTT AGCCCAGTAC
    CCTGGATGGC CTTTGAGGAA TTTTTTGGTC CTAGCAGCCC ACAGATGGAG TAGCAGTTTC
    CAGTCTGTTG AAGTTGTTTG CTTCCGTGAC CGTACCATGC AGGGGGCGAG AGACGTTGCC
    CACAGCATCA TCTTCGAAGT GAAGCTTCCA GAAATGGCAT TTAGCCCAGA TTGTCCTAAA
    GCAGTTGGAT GGGAAAAGAA CCAGAAAGGA GGCATGGGAC CAAGGATGGT GAACCTCAGT
    GAATGTATGG ACCCTAAAAG GTTAGCTGAG TCATCAGTGG ATCTAAATCT CAAACTGATG
    TGTTGGAGAT TGGTTCCTAC TTTAGACTTG GACAAGGTTG TGTCTGTCAA ATGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGCAATGTA GCTAGGACGT TGATGGGTTG GGGCGTGAGA
    CACATCACAT TTGTGGACAA TGCCAAGATC TCCTACTCCA ATCCTGTGAG GCAGCCTCTC
    TATGAGTTTG AAGATTGCCT AGGGGGTGGT AAGCCCAAGG CTCTGGCAGC AGCGGACCGG
    CTCCAGAAAA TATTCCCCGG TGTGAATGCC AGAGGATTCA ACATGAGCAT ACCTATGCCT
    GGGCATCCAG TGAACTTCTC CAGTGTCACT CTGGAGCAAG CCCGCAGAGA TGTGGAGCAA
    CTGGAGCAGC TCATCGAAAG CCATGATGTC GTCTTCCTAT TGATGGACAC CAGGGAGAGC
    CGGTGGCTTC CTGCCGTCAT TGCTGCAAGC AAGAGAAAGC TGGTCATCAA TGCTGCTTTG
    GGATTTGACA CATTTGTTGT CATGAGACAT GGTCTGAAGA AACCAAAGCA GCAAGGAGCT
    GGGGACTTGT GTCCAAACCA CCCTGTGGCA TCTGCTGACC TCCTGGGCTC ATCGCTTTTT
    GCCAACATCC CTGGTTACAA GCTTGGCTGC TACTTCTGCA ATGATGTGGT GGCCCCAGGA
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGTCGTCC AGGACTGGCC
    GTGATTGCAG GAGCCCTGGC CGTGGAATTG ATGGTATCTG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAT CGGATGAATG AGCCTCCAAC CTCTCTTGGG
    CTTGTGCCTC ACCAGATCCG GGGATTTCTT TCACGGTTTG ATAATGTCCT TCCCGTCAGC
    CTGGCATTTG ACAAATGTAC AGCTTGTTCT TCCAAAGTTC TTGATCAATA TGAACGAGAA
    GGATTTAACT TCCTAGCCAA GGTGTTTAAT TCTTCACATT CCTTCTTAGA AGACTTGACT
    GGTCTTACAT TGCTGCATCA AGAAACCCAA GCTGCTGAGA TCTGGGACAT GAGCGATGAT
    GAGACCATCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001336161.1
    CDS473..2584
    Translation

    Target ORF information:

    RefSeq Version NM_001349232.1
    Organism Homo sapiens(human)
    Definition Homo sapiens autophagy related 7 (ATG7), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001349232.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGCGGCAG CTACGGGGGA TCCTGGACTC TCTAAACTGC AGTTTGCCCC TTTTAGTAGT 
    GCCTTGGATG TTGGGTTTTG GCATGAGTTG ACCCAGAAGA AGCTGAACGA GTATCGGCTG
    GATGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAATG GTGACTCTGC TGGGCTGCCA
    GCTCGCTTAA CATTGGAGTT CAGTGCTTTT GACATGAGTG CTCCCACCCC AGCCCGTTGC
    TGCCCAGCTA TTGGAACACT GTATAACACC AACACACTCG AGTCTTTCAA GACTGCAGAT
    AAGAAGCTCC TTTTGGAACA AGCAGCAAAT GAGATATGGG AATCCATAAA ATCAGGCACT
    GCTCTTGAAA ACCCTGTACT CCTCAACAAG TTCCTCCTCT TGACATTTGC AGATCTAAAG
    AAGTACCACT TCTACTATTG GTTTTGCTAT CCTGCCCTCT GTCTTCCAGA GAGTTTACCT
    CTCATTCAGG GGCCAGTGGG TTTGGATCAA AGGTTTTCAC TAAAACAGAT TGAAGCACTA
    GAGTGTGCAT ATGATAATCT TTGTCAAACA GAAGGAGTCA CAGCTCTTCC TTACTTCTTA
    ATCAAGTATG ATGAGAACAT GGTGCTGGTT TCCTTGCTTA AACACTACAG TGATTTCTTC
    CAAGGTCAAA GGACGAAGAT AACAATTGGT GTATATGATC CCTGTAACTT AGCCCAGTAC
    CCTGGATGGC CTTTGAGGAA TTTTTTGGTC CTAGCAGCCC ACAGATGGAG TAGCAGTTTC
    CAGTCTGTTG AAGTTGTTTG CTTCCGTGAC CGTACCATGC AGGGGGCGAG AGACGTTGCC
    CACAGCATCA TCTTCGAAGT GAAGCTTCCA GAAATGGCAT TTAGCCCAGA TTGTCCTAAA
    GCAGTTGGAT GGGAAAAGAA CCAGAAAGGA GGCATGGGAC CAAGGATGGT GAACCTCAGT
    GAATGTATGG ACCCTAAAAG GTTAGCTGAG TCATCAGTGG ATCTAAATCT CAAACTGATG
    TGTTGGAGAT TGGTTCCTAC TTTAGACTTG GACAAGGTTG TGTCTGTCAA ATGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGCAATGTA GCTAGGACGT TGATGGGTTG GGGCGTGAGA
    CACATCACAT TTGTGGACAA TGCCAAGATC TCCTACTCCA ATCCTGTGAG GCAGCCTCTC
    TATGAGTTTG AAGATTGCCT AGGGGGTGGT AAGCCCAAGG CTCTGGCAGC AGCGGACCGG
    CTCCAGAAAA TATTCCCCGG TGTGAATGCC AGAGGATTCA ACATGAGCAT ACCTATGCCT
    GGGCATCCAG TGAACTTCTC CAGTGTCACT CTGGAGCAAG CCCGCAGAGA TGTGGAGCAA
    CTGGAGCAGC TCATCGAAAG CCATGATGTC GTCTTCCTAT TGATGGACAC CAGGGAGAGC
    CGGTGGCTTC CTGCCGTCAT TGCTGCAAGC AAGAGAAAGC TGGTCATCAA TGCTGCTTTG
    GGATTTGACA CATTTGTTGT CATGAGACAT GGTCTGAAGA AACCAAAGCA GCAAGGAGCT
    GGGGACTTGT GTCCAAACCA CCCTGTGGCA TCTGCTGACC TCCTGGGCTC ATCGCTTTTT
    GCCAACATCC CTGGTTACAA GCTTGGCTGC TACTTCTGCA ATGATGTGGT GGCCCCAGGA
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGTCGTCC AGGACTGGCC
    GTGATTGCAG GAGCCCTGGC CGTGGAATTG ATGGTATCTG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAT CGGATGAATG AGCCTCCAAC CTCTCTTGGG
    CTTGTGCCTC ACCAGATCCG GGGATTTCTT TCACGGTTTG ATAATGTCCT TCCCGTCAGC
    CTGGCATTTG ACAAATGTAC AGCTTGTTCT TCCAAAGTTC TTGATCAATA TGAACGAGAA
    GGATTTAACT TCCTAGCCAA GGTGTTTAAT TCTTCACATT CCTTCTTAGA AGACTTGACT
    GGTCTTACAT TGCTGCATCA AGAAACCCAA GCTGCTGAGA TCTGGGACAT GAGCGATGAT
    GAGACCATCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu25185
    Clone ID Related Accession (Same CDS sequence) NM_001349234.1 , NM_001349235.1 , NM_006395.2 , NM_001349233.1 , NM_001349232.1 , NM_001349232.2 , NM_001349233.2 , NM_001349235.2 , NM_001349234.2 , NM_006395.3
    Accession Version NM_001349232.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2112bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-04
    Organism Homo sapiens(human)
    Product ubiquitin-like modifier-activating enzyme ATG7 isoform a
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC083855.3, AC020750.4, AC026185.4 and AC022001.5. On Jun 2, 2019 this sequence version replaced NM_001349232.1. Transcript Variant: This variant (4) represents the longest transcript and encodes the longest isoform (a). Variants 1, 4, 5, 6, and 7 all encode the same isoform (a). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.83723.1, SRR1803615.122845.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGCGGCAG CTACGGGGGA TCCTGGACTC TCTAAACTGC AGTTTGCCCC TTTTAGTAGT 
    GCCTTGGATG TTGGGTTTTG GCATGAGTTG ACCCAGAAGA AGCTGAACGA GTATCGGCTG
    GATGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAATG GTGACTCTGC TGGGCTGCCA
    GCTCGCTTAA CATTGGAGTT CAGTGCTTTT GACATGAGTG CTCCCACCCC AGCCCGTTGC
    TGCCCAGCTA TTGGAACACT GTATAACACC AACACACTCG AGTCTTTCAA GACTGCAGAT
    AAGAAGCTCC TTTTGGAACA AGCAGCAAAT GAGATATGGG AATCCATAAA ATCAGGCACT
    GCTCTTGAAA ACCCTGTACT CCTCAACAAG TTCCTCCTCT TGACATTTGC AGATCTAAAG
    AAGTACCACT TCTACTATTG GTTTTGCTAT CCTGCCCTCT GTCTTCCAGA GAGTTTACCT
    CTCATTCAGG GGCCAGTGGG TTTGGATCAA AGGTTTTCAC TAAAACAGAT TGAAGCACTA
    GAGTGTGCAT ATGATAATCT TTGTCAAACA GAAGGAGTCA CAGCTCTTCC TTACTTCTTA
    ATCAAGTATG ATGAGAACAT GGTGCTGGTT TCCTTGCTTA AACACTACAG TGATTTCTTC
    CAAGGTCAAA GGACGAAGAT AACAATTGGT GTATATGATC CCTGTAACTT AGCCCAGTAC
    CCTGGATGGC CTTTGAGGAA TTTTTTGGTC CTAGCAGCCC ACAGATGGAG TAGCAGTTTC
    CAGTCTGTTG AAGTTGTTTG CTTCCGTGAC CGTACCATGC AGGGGGCGAG AGACGTTGCC
    CACAGCATCA TCTTCGAAGT GAAGCTTCCA GAAATGGCAT TTAGCCCAGA TTGTCCTAAA
    GCAGTTGGAT GGGAAAAGAA CCAGAAAGGA GGCATGGGAC CAAGGATGGT GAACCTCAGT
    GAATGTATGG ACCCTAAAAG GTTAGCTGAG TCATCAGTGG ATCTAAATCT CAAACTGATG
    TGTTGGAGAT TGGTTCCTAC TTTAGACTTG GACAAGGTTG TGTCTGTCAA ATGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGCAATGTA GCTAGGACGT TGATGGGTTG GGGCGTGAGA
    CACATCACAT TTGTGGACAA TGCCAAGATC TCCTACTCCA ATCCTGTGAG GCAGCCTCTC
    TATGAGTTTG AAGATTGCCT AGGGGGTGGT AAGCCCAAGG CTCTGGCAGC AGCGGACCGG
    CTCCAGAAAA TATTCCCCGG TGTGAATGCC AGAGGATTCA ACATGAGCAT ACCTATGCCT
    GGGCATCCAG TGAACTTCTC CAGTGTCACT CTGGAGCAAG CCCGCAGAGA TGTGGAGCAA
    CTGGAGCAGC TCATCGAAAG CCATGATGTC GTCTTCCTAT TGATGGACAC CAGGGAGAGC
    CGGTGGCTTC CTGCCGTCAT TGCTGCAAGC AAGAGAAAGC TGGTCATCAA TGCTGCTTTG
    GGATTTGACA CATTTGTTGT CATGAGACAT GGTCTGAAGA AACCAAAGCA GCAAGGAGCT
    GGGGACTTGT GTCCAAACCA CCCTGTGGCA TCTGCTGACC TCCTGGGCTC ATCGCTTTTT
    GCCAACATCC CTGGTTACAA GCTTGGCTGC TACTTCTGCA ATGATGTGGT GGCCCCAGGA
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGTCGTCC AGGACTGGCC
    GTGATTGCAG GAGCCCTGGC CGTGGAATTG ATGGTATCTG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAT CGGATGAATG AGCCTCCAAC CTCTCTTGGG
    CTTGTGCCTC ACCAGATCCG GGGATTTCTT TCACGGTTTG ATAATGTCCT TCCCGTCAGC
    CTGGCATTTG ACAAATGTAC AGCTTGTTCT TCCAAAGTTC TTGATCAATA TGAACGAGAA
    GGATTTAACT TCCTAGCCAA GGTGTTTAAT TCTTCACATT CCTTCTTAGA AGACTTGACT
    GGTCTTACAT TGCTGCATCA AGAAACCCAA GCTGCTGAGA TCTGGGACAT GAGCGATGAT
    GAGACCATCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001336161.1
    CDS400..2511
    Translation

    Target ORF information:

    RefSeq Version NM_001349232.2
    Organism Homo sapiens(human)
    Definition Homo sapiens autophagy related 7 (ATG7), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001349232.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGCGGCAG CTACGGGGGA TCCTGGACTC TCTAAACTGC AGTTTGCCCC TTTTAGTAGT 
    GCCTTGGATG TTGGGTTTTG GCATGAGTTG ACCCAGAAGA AGCTGAACGA GTATCGGCTG
    GATGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAATG GTGACTCTGC TGGGCTGCCA
    GCTCGCTTAA CATTGGAGTT CAGTGCTTTT GACATGAGTG CTCCCACCCC AGCCCGTTGC
    TGCCCAGCTA TTGGAACACT GTATAACACC AACACACTCG AGTCTTTCAA GACTGCAGAT
    AAGAAGCTCC TTTTGGAACA AGCAGCAAAT GAGATATGGG AATCCATAAA ATCAGGCACT
    GCTCTTGAAA ACCCTGTACT CCTCAACAAG TTCCTCCTCT TGACATTTGC AGATCTAAAG
    AAGTACCACT TCTACTATTG GTTTTGCTAT CCTGCCCTCT GTCTTCCAGA GAGTTTACCT
    CTCATTCAGG GGCCAGTGGG TTTGGATCAA AGGTTTTCAC TAAAACAGAT TGAAGCACTA
    GAGTGTGCAT ATGATAATCT TTGTCAAACA GAAGGAGTCA CAGCTCTTCC TTACTTCTTA
    ATCAAGTATG ATGAGAACAT GGTGCTGGTT TCCTTGCTTA AACACTACAG TGATTTCTTC
    CAAGGTCAAA GGACGAAGAT AACAATTGGT GTATATGATC CCTGTAACTT AGCCCAGTAC
    CCTGGATGGC CTTTGAGGAA TTTTTTGGTC CTAGCAGCCC ACAGATGGAG TAGCAGTTTC
    CAGTCTGTTG AAGTTGTTTG CTTCCGTGAC CGTACCATGC AGGGGGCGAG AGACGTTGCC
    CACAGCATCA TCTTCGAAGT GAAGCTTCCA GAAATGGCAT TTAGCCCAGA TTGTCCTAAA
    GCAGTTGGAT GGGAAAAGAA CCAGAAAGGA GGCATGGGAC CAAGGATGGT GAACCTCAGT
    GAATGTATGG ACCCTAAAAG GTTAGCTGAG TCATCAGTGG ATCTAAATCT CAAACTGATG
    TGTTGGAGAT TGGTTCCTAC TTTAGACTTG GACAAGGTTG TGTCTGTCAA ATGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGCAATGTA GCTAGGACGT TGATGGGTTG GGGCGTGAGA
    CACATCACAT TTGTGGACAA TGCCAAGATC TCCTACTCCA ATCCTGTGAG GCAGCCTCTC
    TATGAGTTTG AAGATTGCCT AGGGGGTGGT AAGCCCAAGG CTCTGGCAGC AGCGGACCGG
    CTCCAGAAAA TATTCCCCGG TGTGAATGCC AGAGGATTCA ACATGAGCAT ACCTATGCCT
    GGGCATCCAG TGAACTTCTC CAGTGTCACT CTGGAGCAAG CCCGCAGAGA TGTGGAGCAA
    CTGGAGCAGC TCATCGAAAG CCATGATGTC GTCTTCCTAT TGATGGACAC CAGGGAGAGC
    CGGTGGCTTC CTGCCGTCAT TGCTGCAAGC AAGAGAAAGC TGGTCATCAA TGCTGCTTTG
    GGATTTGACA CATTTGTTGT CATGAGACAT GGTCTGAAGA AACCAAAGCA GCAAGGAGCT
    GGGGACTTGT GTCCAAACCA CCCTGTGGCA TCTGCTGACC TCCTGGGCTC ATCGCTTTTT
    GCCAACATCC CTGGTTACAA GCTTGGCTGC TACTTCTGCA ATGATGTGGT GGCCCCAGGA
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGTCGTCC AGGACTGGCC
    GTGATTGCAG GAGCCCTGGC CGTGGAATTG ATGGTATCTG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAT CGGATGAATG AGCCTCCAAC CTCTCTTGGG
    CTTGTGCCTC ACCAGATCCG GGGATTTCTT TCACGGTTTG ATAATGTCCT TCCCGTCAGC
    CTGGCATTTG ACAAATGTAC AGCTTGTTCT TCCAAAGTTC TTGATCAATA TGAACGAGAA
    GGATTTAACT TCCTAGCCAA GGTGTTTAAT TCTTCACATT CCTTCTTAGA AGACTTGACT
    GGTCTTACAT TGCTGCATCA AGAAACCCAA GCTGCTGAGA TCTGGGACAT GAGCGATGAT
    GAGACCATCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu25185
    Clone ID Related Accession (Same CDS sequence) NM_001349234.1 , NM_001349235.1 , NM_006395.2 , NM_001349233.1 , NM_001349232.1 , NM_001349232.2 , NM_001349233.2 , NM_001349235.2 , NM_001349234.2 , NM_006395.3
    Accession Version NM_001349233.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2112bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-03
    Organism Homo sapiens(human)
    Product ubiquitin-like modifier-activating enzyme ATG7 isoform a
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC083855.3, AC020750.4, AC026185.4 and AC022001.5. On Jun 2, 2019 this sequence version replaced NM_001349233.1. Transcript Variant: This variant (5), as well as variants 1, 4, 6, and 7, encodes the longest isoform (a). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.5154.1, SRR1660803.66302.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGCGGCAG CTACGGGGGA TCCTGGACTC TCTAAACTGC AGTTTGCCCC TTTTAGTAGT 
    GCCTTGGATG TTGGGTTTTG GCATGAGTTG ACCCAGAAGA AGCTGAACGA GTATCGGCTG
    GATGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAATG GTGACTCTGC TGGGCTGCCA
    GCTCGCTTAA CATTGGAGTT CAGTGCTTTT GACATGAGTG CTCCCACCCC AGCCCGTTGC
    TGCCCAGCTA TTGGAACACT GTATAACACC AACACACTCG AGTCTTTCAA GACTGCAGAT
    AAGAAGCTCC TTTTGGAACA AGCAGCAAAT GAGATATGGG AATCCATAAA ATCAGGCACT
    GCTCTTGAAA ACCCTGTACT CCTCAACAAG TTCCTCCTCT TGACATTTGC AGATCTAAAG
    AAGTACCACT TCTACTATTG GTTTTGCTAT CCTGCCCTCT GTCTTCCAGA GAGTTTACCT
    CTCATTCAGG GGCCAGTGGG TTTGGATCAA AGGTTTTCAC TAAAACAGAT TGAAGCACTA
    GAGTGTGCAT ATGATAATCT TTGTCAAACA GAAGGAGTCA CAGCTCTTCC TTACTTCTTA
    ATCAAGTATG ATGAGAACAT GGTGCTGGTT TCCTTGCTTA AACACTACAG TGATTTCTTC
    CAAGGTCAAA GGACGAAGAT AACAATTGGT GTATATGATC CCTGTAACTT AGCCCAGTAC
    CCTGGATGGC CTTTGAGGAA TTTTTTGGTC CTAGCAGCCC ACAGATGGAG TAGCAGTTTC
    CAGTCTGTTG AAGTTGTTTG CTTCCGTGAC CGTACCATGC AGGGGGCGAG AGACGTTGCC
    CACAGCATCA TCTTCGAAGT GAAGCTTCCA GAAATGGCAT TTAGCCCAGA TTGTCCTAAA
    GCAGTTGGAT GGGAAAAGAA CCAGAAAGGA GGCATGGGAC CAAGGATGGT GAACCTCAGT
    GAATGTATGG ACCCTAAAAG GTTAGCTGAG TCATCAGTGG ATCTAAATCT CAAACTGATG
    TGTTGGAGAT TGGTTCCTAC TTTAGACTTG GACAAGGTTG TGTCTGTCAA ATGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGCAATGTA GCTAGGACGT TGATGGGTTG GGGCGTGAGA
    CACATCACAT TTGTGGACAA TGCCAAGATC TCCTACTCCA ATCCTGTGAG GCAGCCTCTC
    TATGAGTTTG AAGATTGCCT AGGGGGTGGT AAGCCCAAGG CTCTGGCAGC AGCGGACCGG
    CTCCAGAAAA TATTCCCCGG TGTGAATGCC AGAGGATTCA ACATGAGCAT ACCTATGCCT
    GGGCATCCAG TGAACTTCTC CAGTGTCACT CTGGAGCAAG CCCGCAGAGA TGTGGAGCAA
    CTGGAGCAGC TCATCGAAAG CCATGATGTC GTCTTCCTAT TGATGGACAC CAGGGAGAGC
    CGGTGGCTTC CTGCCGTCAT TGCTGCAAGC AAGAGAAAGC TGGTCATCAA TGCTGCTTTG
    GGATTTGACA CATTTGTTGT CATGAGACAT GGTCTGAAGA AACCAAAGCA GCAAGGAGCT
    GGGGACTTGT GTCCAAACCA CCCTGTGGCA TCTGCTGACC TCCTGGGCTC ATCGCTTTTT
    GCCAACATCC CTGGTTACAA GCTTGGCTGC TACTTCTGCA ATGATGTGGT GGCCCCAGGA
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGTCGTCC AGGACTGGCC
    GTGATTGCAG GAGCCCTGGC CGTGGAATTG ATGGTATCTG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAT CGGATGAATG AGCCTCCAAC CTCTCTTGGG
    CTTGTGCCTC ACCAGATCCG GGGATTTCTT TCACGGTTTG ATAATGTCCT TCCCGTCAGC
    CTGGCATTTG ACAAATGTAC AGCTTGTTCT TCCAAAGTTC TTGATCAATA TGAACGAGAA
    GGATTTAACT TCCTAGCCAA GGTGTTTAAT TCTTCACATT CCTTCTTAGA AGACTTGACT
    GGTCTTACAT TGCTGCATCA AGAAACCCAA GCTGCTGAGA TCTGGGACAT GAGCGATGAT
    GAGACCATCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001336162.1
    CDS154..2265
    Translation

    Target ORF information:

    RefSeq Version NM_001349233.2
    Organism Homo sapiens(human)
    Definition Homo sapiens autophagy related 7 (ATG7), transcript variant 5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001349233.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGCGGCAG CTACGGGGGA TCCTGGACTC TCTAAACTGC AGTTTGCCCC TTTTAGTAGT 
    GCCTTGGATG TTGGGTTTTG GCATGAGTTG ACCCAGAAGA AGCTGAACGA GTATCGGCTG
    GATGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAATG GTGACTCTGC TGGGCTGCCA
    GCTCGCTTAA CATTGGAGTT CAGTGCTTTT GACATGAGTG CTCCCACCCC AGCCCGTTGC
    TGCCCAGCTA TTGGAACACT GTATAACACC AACACACTCG AGTCTTTCAA GACTGCAGAT
    AAGAAGCTCC TTTTGGAACA AGCAGCAAAT GAGATATGGG AATCCATAAA ATCAGGCACT
    GCTCTTGAAA ACCCTGTACT CCTCAACAAG TTCCTCCTCT TGACATTTGC AGATCTAAAG
    AAGTACCACT TCTACTATTG GTTTTGCTAT CCTGCCCTCT GTCTTCCAGA GAGTTTACCT
    CTCATTCAGG GGCCAGTGGG TTTGGATCAA AGGTTTTCAC TAAAACAGAT TGAAGCACTA
    GAGTGTGCAT ATGATAATCT TTGTCAAACA GAAGGAGTCA CAGCTCTTCC TTACTTCTTA
    ATCAAGTATG ATGAGAACAT GGTGCTGGTT TCCTTGCTTA AACACTACAG TGATTTCTTC
    CAAGGTCAAA GGACGAAGAT AACAATTGGT GTATATGATC CCTGTAACTT AGCCCAGTAC
    CCTGGATGGC CTTTGAGGAA TTTTTTGGTC CTAGCAGCCC ACAGATGGAG TAGCAGTTTC
    CAGTCTGTTG AAGTTGTTTG CTTCCGTGAC CGTACCATGC AGGGGGCGAG AGACGTTGCC
    CACAGCATCA TCTTCGAAGT GAAGCTTCCA GAAATGGCAT TTAGCCCAGA TTGTCCTAAA
    GCAGTTGGAT GGGAAAAGAA CCAGAAAGGA GGCATGGGAC CAAGGATGGT GAACCTCAGT
    GAATGTATGG ACCCTAAAAG GTTAGCTGAG TCATCAGTGG ATCTAAATCT CAAACTGATG
    TGTTGGAGAT TGGTTCCTAC TTTAGACTTG GACAAGGTTG TGTCTGTCAA ATGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGCAATGTA GCTAGGACGT TGATGGGTTG GGGCGTGAGA
    CACATCACAT TTGTGGACAA TGCCAAGATC TCCTACTCCA ATCCTGTGAG GCAGCCTCTC
    TATGAGTTTG AAGATTGCCT AGGGGGTGGT AAGCCCAAGG CTCTGGCAGC AGCGGACCGG
    CTCCAGAAAA TATTCCCCGG TGTGAATGCC AGAGGATTCA ACATGAGCAT ACCTATGCCT
    GGGCATCCAG TGAACTTCTC CAGTGTCACT CTGGAGCAAG CCCGCAGAGA TGTGGAGCAA
    CTGGAGCAGC TCATCGAAAG CCATGATGTC GTCTTCCTAT TGATGGACAC CAGGGAGAGC
    CGGTGGCTTC CTGCCGTCAT TGCTGCAAGC AAGAGAAAGC TGGTCATCAA TGCTGCTTTG
    GGATTTGACA CATTTGTTGT CATGAGACAT GGTCTGAAGA AACCAAAGCA GCAAGGAGCT
    GGGGACTTGT GTCCAAACCA CCCTGTGGCA TCTGCTGACC TCCTGGGCTC ATCGCTTTTT
    GCCAACATCC CTGGTTACAA GCTTGGCTGC TACTTCTGCA ATGATGTGGT GGCCCCAGGA
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGTCGTCC AGGACTGGCC
    GTGATTGCAG GAGCCCTGGC CGTGGAATTG ATGGTATCTG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAT CGGATGAATG AGCCTCCAAC CTCTCTTGGG
    CTTGTGCCTC ACCAGATCCG GGGATTTCTT TCACGGTTTG ATAATGTCCT TCCCGTCAGC
    CTGGCATTTG ACAAATGTAC AGCTTGTTCT TCCAAAGTTC TTGATCAATA TGAACGAGAA
    GGATTTAACT TCCTAGCCAA GGTGTTTAAT TCTTCACATT CCTTCTTAGA AGACTTGACT
    GGTCTTACAT TGCTGCATCA AGAAACCCAA GCTGCTGAGA TCTGGGACAT GAGCGATGAT
    GAGACCATCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu25185
    Clone ID Related Accession (Same CDS sequence) NM_001349234.1 , NM_001349235.1 , NM_006395.2 , NM_001349233.1 , NM_001349232.1 , NM_001349232.2 , NM_001349233.2 , NM_001349235.2 , NM_001349234.2 , NM_006395.3
    Accession Version NM_001349235.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2112bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-03
    Organism Homo sapiens(human)
    Product ubiquitin-like modifier-activating enzyme ATG7 isoform a
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC083855.3, AC020750.4, AC026185.4 and AC022001.5. On Jun 2, 2019 this sequence version replaced NM_001349235.1. Transcript Variant: This variant (7), as well as variants 1, 4, 5, and 6, encodes the longest isoform (a). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.272133.1, SRR1660805.46133.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGCGGCAG CTACGGGGGA TCCTGGACTC TCTAAACTGC AGTTTGCCCC TTTTAGTAGT 
    GCCTTGGATG TTGGGTTTTG GCATGAGTTG ACCCAGAAGA AGCTGAACGA GTATCGGCTG
    GATGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAATG GTGACTCTGC TGGGCTGCCA
    GCTCGCTTAA CATTGGAGTT CAGTGCTTTT GACATGAGTG CTCCCACCCC AGCCCGTTGC
    TGCCCAGCTA TTGGAACACT GTATAACACC AACACACTCG AGTCTTTCAA GACTGCAGAT
    AAGAAGCTCC TTTTGGAACA AGCAGCAAAT GAGATATGGG AATCCATAAA ATCAGGCACT
    GCTCTTGAAA ACCCTGTACT CCTCAACAAG TTCCTCCTCT TGACATTTGC AGATCTAAAG
    AAGTACCACT TCTACTATTG GTTTTGCTAT CCTGCCCTCT GTCTTCCAGA GAGTTTACCT
    CTCATTCAGG GGCCAGTGGG TTTGGATCAA AGGTTTTCAC TAAAACAGAT TGAAGCACTA
    GAGTGTGCAT ATGATAATCT TTGTCAAACA GAAGGAGTCA CAGCTCTTCC TTACTTCTTA
    ATCAAGTATG ATGAGAACAT GGTGCTGGTT TCCTTGCTTA AACACTACAG TGATTTCTTC
    CAAGGTCAAA GGACGAAGAT AACAATTGGT GTATATGATC CCTGTAACTT AGCCCAGTAC
    CCTGGATGGC CTTTGAGGAA TTTTTTGGTC CTAGCAGCCC ACAGATGGAG TAGCAGTTTC
    CAGTCTGTTG AAGTTGTTTG CTTCCGTGAC CGTACCATGC AGGGGGCGAG AGACGTTGCC
    CACAGCATCA TCTTCGAAGT GAAGCTTCCA GAAATGGCAT TTAGCCCAGA TTGTCCTAAA
    GCAGTTGGAT GGGAAAAGAA CCAGAAAGGA GGCATGGGAC CAAGGATGGT GAACCTCAGT
    GAATGTATGG ACCCTAAAAG GTTAGCTGAG TCATCAGTGG ATCTAAATCT CAAACTGATG
    TGTTGGAGAT TGGTTCCTAC TTTAGACTTG GACAAGGTTG TGTCTGTCAA ATGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGCAATGTA GCTAGGACGT TGATGGGTTG GGGCGTGAGA
    CACATCACAT TTGTGGACAA TGCCAAGATC TCCTACTCCA ATCCTGTGAG GCAGCCTCTC
    TATGAGTTTG AAGATTGCCT AGGGGGTGGT AAGCCCAAGG CTCTGGCAGC AGCGGACCGG
    CTCCAGAAAA TATTCCCCGG TGTGAATGCC AGAGGATTCA ACATGAGCAT ACCTATGCCT
    GGGCATCCAG TGAACTTCTC CAGTGTCACT CTGGAGCAAG CCCGCAGAGA TGTGGAGCAA
    CTGGAGCAGC TCATCGAAAG CCATGATGTC GTCTTCCTAT TGATGGACAC CAGGGAGAGC
    CGGTGGCTTC CTGCCGTCAT TGCTGCAAGC AAGAGAAAGC TGGTCATCAA TGCTGCTTTG
    GGATTTGACA CATTTGTTGT CATGAGACAT GGTCTGAAGA AACCAAAGCA GCAAGGAGCT
    GGGGACTTGT GTCCAAACCA CCCTGTGGCA TCTGCTGACC TCCTGGGCTC ATCGCTTTTT
    GCCAACATCC CTGGTTACAA GCTTGGCTGC TACTTCTGCA ATGATGTGGT GGCCCCAGGA
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGTCGTCC AGGACTGGCC
    GTGATTGCAG GAGCCCTGGC CGTGGAATTG ATGGTATCTG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAT CGGATGAATG AGCCTCCAAC CTCTCTTGGG
    CTTGTGCCTC ACCAGATCCG GGGATTTCTT TCACGGTTTG ATAATGTCCT TCCCGTCAGC
    CTGGCATTTG ACAAATGTAC AGCTTGTTCT TCCAAAGTTC TTGATCAATA TGAACGAGAA
    GGATTTAACT TCCTAGCCAA GGTGTTTAAT TCTTCACATT CCTTCTTAGA AGACTTGACT
    GGTCTTACAT TGCTGCATCA AGAAACCCAA GCTGCTGAGA TCTGGGACAT GAGCGATGAT
    GAGACCATCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001336164.1
    CDS291..2402
    Translation

    Target ORF information:

    RefSeq Version NM_001349235.2
    Organism Homo sapiens(human)
    Definition Homo sapiens autophagy related 7 (ATG7), transcript variant 7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001349235.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGCGGCAG CTACGGGGGA TCCTGGACTC TCTAAACTGC AGTTTGCCCC TTTTAGTAGT 
    GCCTTGGATG TTGGGTTTTG GCATGAGTTG ACCCAGAAGA AGCTGAACGA GTATCGGCTG
    GATGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAATG GTGACTCTGC TGGGCTGCCA
    GCTCGCTTAA CATTGGAGTT CAGTGCTTTT GACATGAGTG CTCCCACCCC AGCCCGTTGC
    TGCCCAGCTA TTGGAACACT GTATAACACC AACACACTCG AGTCTTTCAA GACTGCAGAT
    AAGAAGCTCC TTTTGGAACA AGCAGCAAAT GAGATATGGG AATCCATAAA ATCAGGCACT
    GCTCTTGAAA ACCCTGTACT CCTCAACAAG TTCCTCCTCT TGACATTTGC AGATCTAAAG
    AAGTACCACT TCTACTATTG GTTTTGCTAT CCTGCCCTCT GTCTTCCAGA GAGTTTACCT
    CTCATTCAGG GGCCAGTGGG TTTGGATCAA AGGTTTTCAC TAAAACAGAT TGAAGCACTA
    GAGTGTGCAT ATGATAATCT TTGTCAAACA GAAGGAGTCA CAGCTCTTCC TTACTTCTTA
    ATCAAGTATG ATGAGAACAT GGTGCTGGTT TCCTTGCTTA AACACTACAG TGATTTCTTC
    CAAGGTCAAA GGACGAAGAT AACAATTGGT GTATATGATC CCTGTAACTT AGCCCAGTAC
    CCTGGATGGC CTTTGAGGAA TTTTTTGGTC CTAGCAGCCC ACAGATGGAG TAGCAGTTTC
    CAGTCTGTTG AAGTTGTTTG CTTCCGTGAC CGTACCATGC AGGGGGCGAG AGACGTTGCC
    CACAGCATCA TCTTCGAAGT GAAGCTTCCA GAAATGGCAT TTAGCCCAGA TTGTCCTAAA
    GCAGTTGGAT GGGAAAAGAA CCAGAAAGGA GGCATGGGAC CAAGGATGGT GAACCTCAGT
    GAATGTATGG ACCCTAAAAG GTTAGCTGAG TCATCAGTGG ATCTAAATCT CAAACTGATG
    TGTTGGAGAT TGGTTCCTAC TTTAGACTTG GACAAGGTTG TGTCTGTCAA ATGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGCAATGTA GCTAGGACGT TGATGGGTTG GGGCGTGAGA
    CACATCACAT TTGTGGACAA TGCCAAGATC TCCTACTCCA ATCCTGTGAG GCAGCCTCTC
    TATGAGTTTG AAGATTGCCT AGGGGGTGGT AAGCCCAAGG CTCTGGCAGC AGCGGACCGG
    CTCCAGAAAA TATTCCCCGG TGTGAATGCC AGAGGATTCA ACATGAGCAT ACCTATGCCT
    GGGCATCCAG TGAACTTCTC CAGTGTCACT CTGGAGCAAG CCCGCAGAGA TGTGGAGCAA
    CTGGAGCAGC TCATCGAAAG CCATGATGTC GTCTTCCTAT TGATGGACAC CAGGGAGAGC
    CGGTGGCTTC CTGCCGTCAT TGCTGCAAGC AAGAGAAAGC TGGTCATCAA TGCTGCTTTG
    GGATTTGACA CATTTGTTGT CATGAGACAT GGTCTGAAGA AACCAAAGCA GCAAGGAGCT
    GGGGACTTGT GTCCAAACCA CCCTGTGGCA TCTGCTGACC TCCTGGGCTC ATCGCTTTTT
    GCCAACATCC CTGGTTACAA GCTTGGCTGC TACTTCTGCA ATGATGTGGT GGCCCCAGGA
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGTCGTCC AGGACTGGCC
    GTGATTGCAG GAGCCCTGGC CGTGGAATTG ATGGTATCTG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAT CGGATGAATG AGCCTCCAAC CTCTCTTGGG
    CTTGTGCCTC ACCAGATCCG GGGATTTCTT TCACGGTTTG ATAATGTCCT TCCCGTCAGC
    CTGGCATTTG ACAAATGTAC AGCTTGTTCT TCCAAAGTTC TTGATCAATA TGAACGAGAA
    GGATTTAACT TCCTAGCCAA GGTGTTTAAT TCTTCACATT CCTTCTTAGA AGACTTGACT
    GGTCTTACAT TGCTGCATCA AGAAACCCAA GCTGCTGAGA TCTGGGACAT GAGCGATGAT
    GAGACCATCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu25185
    Clone ID Related Accession (Same CDS sequence) NM_001349234.1 , NM_001349235.1 , NM_006395.2 , NM_001349233.1 , NM_001349232.1 , NM_001349232.2 , NM_001349233.2 , NM_001349235.2 , NM_001349234.2 , NM_006395.3
    Accession Version NM_001349234.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2112bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-04
    Organism Homo sapiens(human)
    Product ubiquitin-like modifier-activating enzyme ATG7 isoform a
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC083855.3, AC020750.4, AC026185.4 and AC022001.5. On Jun 2, 2019 this sequence version replaced NM_001349234.1. Transcript Variant: This variant (6), as well as variants 1, 4, 5, and 7, encodes the longest isoform (a). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.32467.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGCGGCAG CTACGGGGGA TCCTGGACTC TCTAAACTGC AGTTTGCCCC TTTTAGTAGT 
    GCCTTGGATG TTGGGTTTTG GCATGAGTTG ACCCAGAAGA AGCTGAACGA GTATCGGCTG
    GATGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAATG GTGACTCTGC TGGGCTGCCA
    GCTCGCTTAA CATTGGAGTT CAGTGCTTTT GACATGAGTG CTCCCACCCC AGCCCGTTGC
    TGCCCAGCTA TTGGAACACT GTATAACACC AACACACTCG AGTCTTTCAA GACTGCAGAT
    AAGAAGCTCC TTTTGGAACA AGCAGCAAAT GAGATATGGG AATCCATAAA ATCAGGCACT
    GCTCTTGAAA ACCCTGTACT CCTCAACAAG TTCCTCCTCT TGACATTTGC AGATCTAAAG
    AAGTACCACT TCTACTATTG GTTTTGCTAT CCTGCCCTCT GTCTTCCAGA GAGTTTACCT
    CTCATTCAGG GGCCAGTGGG TTTGGATCAA AGGTTTTCAC TAAAACAGAT TGAAGCACTA
    GAGTGTGCAT ATGATAATCT TTGTCAAACA GAAGGAGTCA CAGCTCTTCC TTACTTCTTA
    ATCAAGTATG ATGAGAACAT GGTGCTGGTT TCCTTGCTTA AACACTACAG TGATTTCTTC
    CAAGGTCAAA GGACGAAGAT AACAATTGGT GTATATGATC CCTGTAACTT AGCCCAGTAC
    CCTGGATGGC CTTTGAGGAA TTTTTTGGTC CTAGCAGCCC ACAGATGGAG TAGCAGTTTC
    CAGTCTGTTG AAGTTGTTTG CTTCCGTGAC CGTACCATGC AGGGGGCGAG AGACGTTGCC
    CACAGCATCA TCTTCGAAGT GAAGCTTCCA GAAATGGCAT TTAGCCCAGA TTGTCCTAAA
    GCAGTTGGAT GGGAAAAGAA CCAGAAAGGA GGCATGGGAC CAAGGATGGT GAACCTCAGT
    GAATGTATGG ACCCTAAAAG GTTAGCTGAG TCATCAGTGG ATCTAAATCT CAAACTGATG
    TGTTGGAGAT TGGTTCCTAC TTTAGACTTG GACAAGGTTG TGTCTGTCAA ATGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGCAATGTA GCTAGGACGT TGATGGGTTG GGGCGTGAGA
    CACATCACAT TTGTGGACAA TGCCAAGATC TCCTACTCCA ATCCTGTGAG GCAGCCTCTC
    TATGAGTTTG AAGATTGCCT AGGGGGTGGT AAGCCCAAGG CTCTGGCAGC AGCGGACCGG
    CTCCAGAAAA TATTCCCCGG TGTGAATGCC AGAGGATTCA ACATGAGCAT ACCTATGCCT
    GGGCATCCAG TGAACTTCTC CAGTGTCACT CTGGAGCAAG CCCGCAGAGA TGTGGAGCAA
    CTGGAGCAGC TCATCGAAAG CCATGATGTC GTCTTCCTAT TGATGGACAC CAGGGAGAGC
    CGGTGGCTTC CTGCCGTCAT TGCTGCAAGC AAGAGAAAGC TGGTCATCAA TGCTGCTTTG
    GGATTTGACA CATTTGTTGT CATGAGACAT GGTCTGAAGA AACCAAAGCA GCAAGGAGCT
    GGGGACTTGT GTCCAAACCA CCCTGTGGCA TCTGCTGACC TCCTGGGCTC ATCGCTTTTT
    GCCAACATCC CTGGTTACAA GCTTGGCTGC TACTTCTGCA ATGATGTGGT GGCCCCAGGA
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGTCGTCC AGGACTGGCC
    GTGATTGCAG GAGCCCTGGC CGTGGAATTG ATGGTATCTG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAT CGGATGAATG AGCCTCCAAC CTCTCTTGGG
    CTTGTGCCTC ACCAGATCCG GGGATTTCTT TCACGGTTTG ATAATGTCCT TCCCGTCAGC
    CTGGCATTTG ACAAATGTAC AGCTTGTTCT TCCAAAGTTC TTGATCAATA TGAACGAGAA
    GGATTTAACT TCCTAGCCAA GGTGTTTAAT TCTTCACATT CCTTCTTAGA AGACTTGACT
    GGTCTTACAT TGCTGCATCA AGAAACCCAA GCTGCTGAGA TCTGGGACAT GAGCGATGAT
    GAGACCATCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001336163.1
    CDS140..2251
    Translation

    Target ORF information:

    RefSeq Version NM_001349234.2
    Organism Homo sapiens(human)
    Definition Homo sapiens autophagy related 7 (ATG7), transcript variant 6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001349234.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGCGGCAG CTACGGGGGA TCCTGGACTC TCTAAACTGC AGTTTGCCCC TTTTAGTAGT 
    GCCTTGGATG TTGGGTTTTG GCATGAGTTG ACCCAGAAGA AGCTGAACGA GTATCGGCTG
    GATGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAATG GTGACTCTGC TGGGCTGCCA
    GCTCGCTTAA CATTGGAGTT CAGTGCTTTT GACATGAGTG CTCCCACCCC AGCCCGTTGC
    TGCCCAGCTA TTGGAACACT GTATAACACC AACACACTCG AGTCTTTCAA GACTGCAGAT
    AAGAAGCTCC TTTTGGAACA AGCAGCAAAT GAGATATGGG AATCCATAAA ATCAGGCACT
    GCTCTTGAAA ACCCTGTACT CCTCAACAAG TTCCTCCTCT TGACATTTGC AGATCTAAAG
    AAGTACCACT TCTACTATTG GTTTTGCTAT CCTGCCCTCT GTCTTCCAGA GAGTTTACCT
    CTCATTCAGG GGCCAGTGGG TTTGGATCAA AGGTTTTCAC TAAAACAGAT TGAAGCACTA
    GAGTGTGCAT ATGATAATCT TTGTCAAACA GAAGGAGTCA CAGCTCTTCC TTACTTCTTA
    ATCAAGTATG ATGAGAACAT GGTGCTGGTT TCCTTGCTTA AACACTACAG TGATTTCTTC
    CAAGGTCAAA GGACGAAGAT AACAATTGGT GTATATGATC CCTGTAACTT AGCCCAGTAC
    CCTGGATGGC CTTTGAGGAA TTTTTTGGTC CTAGCAGCCC ACAGATGGAG TAGCAGTTTC
    CAGTCTGTTG AAGTTGTTTG CTTCCGTGAC CGTACCATGC AGGGGGCGAG AGACGTTGCC
    CACAGCATCA TCTTCGAAGT GAAGCTTCCA GAAATGGCAT TTAGCCCAGA TTGTCCTAAA
    GCAGTTGGAT GGGAAAAGAA CCAGAAAGGA GGCATGGGAC CAAGGATGGT GAACCTCAGT
    GAATGTATGG ACCCTAAAAG GTTAGCTGAG TCATCAGTGG ATCTAAATCT CAAACTGATG
    TGTTGGAGAT TGGTTCCTAC TTTAGACTTG GACAAGGTTG TGTCTGTCAA ATGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGCAATGTA GCTAGGACGT TGATGGGTTG GGGCGTGAGA
    CACATCACAT TTGTGGACAA TGCCAAGATC TCCTACTCCA ATCCTGTGAG GCAGCCTCTC
    TATGAGTTTG AAGATTGCCT AGGGGGTGGT AAGCCCAAGG CTCTGGCAGC AGCGGACCGG
    CTCCAGAAAA TATTCCCCGG TGTGAATGCC AGAGGATTCA ACATGAGCAT ACCTATGCCT
    GGGCATCCAG TGAACTTCTC CAGTGTCACT CTGGAGCAAG CCCGCAGAGA TGTGGAGCAA
    CTGGAGCAGC TCATCGAAAG CCATGATGTC GTCTTCCTAT TGATGGACAC CAGGGAGAGC
    CGGTGGCTTC CTGCCGTCAT TGCTGCAAGC AAGAGAAAGC TGGTCATCAA TGCTGCTTTG
    GGATTTGACA CATTTGTTGT CATGAGACAT GGTCTGAAGA AACCAAAGCA GCAAGGAGCT
    GGGGACTTGT GTCCAAACCA CCCTGTGGCA TCTGCTGACC TCCTGGGCTC ATCGCTTTTT
    GCCAACATCC CTGGTTACAA GCTTGGCTGC TACTTCTGCA ATGATGTGGT GGCCCCAGGA
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGTCGTCC AGGACTGGCC
    GTGATTGCAG GAGCCCTGGC CGTGGAATTG ATGGTATCTG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAT CGGATGAATG AGCCTCCAAC CTCTCTTGGG
    CTTGTGCCTC ACCAGATCCG GGGATTTCTT TCACGGTTTG ATAATGTCCT TCCCGTCAGC
    CTGGCATTTG ACAAATGTAC AGCTTGTTCT TCCAAAGTTC TTGATCAATA TGAACGAGAA
    GGATTTAACT TCCTAGCCAA GGTGTTTAAT TCTTCACATT CCTTCTTAGA AGACTTGACT
    GGTCTTACAT TGCTGCATCA AGAAACCCAA GCTGCTGAGA TCTGGGACAT GAGCGATGAT
    GAGACCATCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu25185
    Clone ID Related Accession (Same CDS sequence) NM_001349234.1 , NM_001349235.1 , NM_006395.2 , NM_001349233.1 , NM_001349232.1 , NM_001349232.2 , NM_001349233.2 , NM_001349235.2 , NM_001349234.2 , NM_006395.3
    Accession Version NM_006395.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2112bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-04
    Organism Homo sapiens(human)
    Product ubiquitin-like modifier-activating enzyme ATG7 isoform a
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA177126.1, BC000091.2, AK075221.1, AL122075.1 and AC022001.5. On Jun 2, 2019 this sequence version replaced NM_006395.2. Transcript Variant: This variant (1), as well as variants 4, 5, 6, and 7, encodes the longest isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.253223.1, SRR1803612.155068.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGCGGCAG CTACGGGGGA TCCTGGACTC TCTAAACTGC AGTTTGCCCC TTTTAGTAGT 
    GCCTTGGATG TTGGGTTTTG GCATGAGTTG ACCCAGAAGA AGCTGAACGA GTATCGGCTG
    GATGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAATG GTGACTCTGC TGGGCTGCCA
    GCTCGCTTAA CATTGGAGTT CAGTGCTTTT GACATGAGTG CTCCCACCCC AGCCCGTTGC
    TGCCCAGCTA TTGGAACACT GTATAACACC AACACACTCG AGTCTTTCAA GACTGCAGAT
    AAGAAGCTCC TTTTGGAACA AGCAGCAAAT GAGATATGGG AATCCATAAA ATCAGGCACT
    GCTCTTGAAA ACCCTGTACT CCTCAACAAG TTCCTCCTCT TGACATTTGC AGATCTAAAG
    AAGTACCACT TCTACTATTG GTTTTGCTAT CCTGCCCTCT GTCTTCCAGA GAGTTTACCT
    CTCATTCAGG GGCCAGTGGG TTTGGATCAA AGGTTTTCAC TAAAACAGAT TGAAGCACTA
    GAGTGTGCAT ATGATAATCT TTGTCAAACA GAAGGAGTCA CAGCTCTTCC TTACTTCTTA
    ATCAAGTATG ATGAGAACAT GGTGCTGGTT TCCTTGCTTA AACACTACAG TGATTTCTTC
    CAAGGTCAAA GGACGAAGAT AACAATTGGT GTATATGATC CCTGTAACTT AGCCCAGTAC
    CCTGGATGGC CTTTGAGGAA TTTTTTGGTC CTAGCAGCCC ACAGATGGAG TAGCAGTTTC
    CAGTCTGTTG AAGTTGTTTG CTTCCGTGAC CGTACCATGC AGGGGGCGAG AGACGTTGCC
    CACAGCATCA TCTTCGAAGT GAAGCTTCCA GAAATGGCAT TTAGCCCAGA TTGTCCTAAA
    GCAGTTGGAT GGGAAAAGAA CCAGAAAGGA GGCATGGGAC CAAGGATGGT GAACCTCAGT
    GAATGTATGG ACCCTAAAAG GTTAGCTGAG TCATCAGTGG ATCTAAATCT CAAACTGATG
    TGTTGGAGAT TGGTTCCTAC TTTAGACTTG GACAAGGTTG TGTCTGTCAA ATGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGCAATGTA GCTAGGACGT TGATGGGTTG GGGCGTGAGA
    CACATCACAT TTGTGGACAA TGCCAAGATC TCCTACTCCA ATCCTGTGAG GCAGCCTCTC
    TATGAGTTTG AAGATTGCCT AGGGGGTGGT AAGCCCAAGG CTCTGGCAGC AGCGGACCGG
    CTCCAGAAAA TATTCCCCGG TGTGAATGCC AGAGGATTCA ACATGAGCAT ACCTATGCCT
    GGGCATCCAG TGAACTTCTC CAGTGTCACT CTGGAGCAAG CCCGCAGAGA TGTGGAGCAA
    CTGGAGCAGC TCATCGAAAG CCATGATGTC GTCTTCCTAT TGATGGACAC CAGGGAGAGC
    CGGTGGCTTC CTGCCGTCAT TGCTGCAAGC AAGAGAAAGC TGGTCATCAA TGCTGCTTTG
    GGATTTGACA CATTTGTTGT CATGAGACAT GGTCTGAAGA AACCAAAGCA GCAAGGAGCT
    GGGGACTTGT GTCCAAACCA CCCTGTGGCA TCTGCTGACC TCCTGGGCTC ATCGCTTTTT
    GCCAACATCC CTGGTTACAA GCTTGGCTGC TACTTCTGCA ATGATGTGGT GGCCCCAGGA
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGTCGTCC AGGACTGGCC
    GTGATTGCAG GAGCCCTGGC CGTGGAATTG ATGGTATCTG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAT CGGATGAATG AGCCTCCAAC CTCTCTTGGG
    CTTGTGCCTC ACCAGATCCG GGGATTTCTT TCACGGTTTG ATAATGTCCT TCCCGTCAGC
    CTGGCATTTG ACAAATGTAC AGCTTGTTCT TCCAAAGTTC TTGATCAATA TGAACGAGAA
    GGATTTAACT TCCTAGCCAA GGTGTTTAAT TCTTCACATT CCTTCTTAGA AGACTTGACT
    GGTCTTACAT TGCTGCATCA AGAAACCCAA GCTGCTGAGA TCTGGGACAT GAGCGATGAT
    GAGACCATCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_006386.1
    CDS45..2156
    Translation

    Target ORF information:

    RefSeq Version NM_006395.3
    Organism Homo sapiens(human)
    Definition Homo sapiens autophagy related 7 (ATG7), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_006395.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGCGGCAG CTACGGGGGA TCCTGGACTC TCTAAACTGC AGTTTGCCCC TTTTAGTAGT 
    GCCTTGGATG TTGGGTTTTG GCATGAGTTG ACCCAGAAGA AGCTGAACGA GTATCGGCTG
    GATGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAATG GTGACTCTGC TGGGCTGCCA
    GCTCGCTTAA CATTGGAGTT CAGTGCTTTT GACATGAGTG CTCCCACCCC AGCCCGTTGC
    TGCCCAGCTA TTGGAACACT GTATAACACC AACACACTCG AGTCTTTCAA GACTGCAGAT
    AAGAAGCTCC TTTTGGAACA AGCAGCAAAT GAGATATGGG AATCCATAAA ATCAGGCACT
    GCTCTTGAAA ACCCTGTACT CCTCAACAAG TTCCTCCTCT TGACATTTGC AGATCTAAAG
    AAGTACCACT TCTACTATTG GTTTTGCTAT CCTGCCCTCT GTCTTCCAGA GAGTTTACCT
    CTCATTCAGG GGCCAGTGGG TTTGGATCAA AGGTTTTCAC TAAAACAGAT TGAAGCACTA
    GAGTGTGCAT ATGATAATCT TTGTCAAACA GAAGGAGTCA CAGCTCTTCC TTACTTCTTA
    ATCAAGTATG ATGAGAACAT GGTGCTGGTT TCCTTGCTTA AACACTACAG TGATTTCTTC
    CAAGGTCAAA GGACGAAGAT AACAATTGGT GTATATGATC CCTGTAACTT AGCCCAGTAC
    CCTGGATGGC CTTTGAGGAA TTTTTTGGTC CTAGCAGCCC ACAGATGGAG TAGCAGTTTC
    CAGTCTGTTG AAGTTGTTTG CTTCCGTGAC CGTACCATGC AGGGGGCGAG AGACGTTGCC
    CACAGCATCA TCTTCGAAGT GAAGCTTCCA GAAATGGCAT TTAGCCCAGA TTGTCCTAAA
    GCAGTTGGAT GGGAAAAGAA CCAGAAAGGA GGCATGGGAC CAAGGATGGT GAACCTCAGT
    GAATGTATGG ACCCTAAAAG GTTAGCTGAG TCATCAGTGG ATCTAAATCT CAAACTGATG
    TGTTGGAGAT TGGTTCCTAC TTTAGACTTG GACAAGGTTG TGTCTGTCAA ATGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGCAATGTA GCTAGGACGT TGATGGGTTG GGGCGTGAGA
    CACATCACAT TTGTGGACAA TGCCAAGATC TCCTACTCCA ATCCTGTGAG GCAGCCTCTC
    TATGAGTTTG AAGATTGCCT AGGGGGTGGT AAGCCCAAGG CTCTGGCAGC AGCGGACCGG
    CTCCAGAAAA TATTCCCCGG TGTGAATGCC AGAGGATTCA ACATGAGCAT ACCTATGCCT
    GGGCATCCAG TGAACTTCTC CAGTGTCACT CTGGAGCAAG CCCGCAGAGA TGTGGAGCAA
    CTGGAGCAGC TCATCGAAAG CCATGATGTC GTCTTCCTAT TGATGGACAC CAGGGAGAGC
    CGGTGGCTTC CTGCCGTCAT TGCTGCAAGC AAGAGAAAGC TGGTCATCAA TGCTGCTTTG
    GGATTTGACA CATTTGTTGT CATGAGACAT GGTCTGAAGA AACCAAAGCA GCAAGGAGCT
    GGGGACTTGT GTCCAAACCA CCCTGTGGCA TCTGCTGACC TCCTGGGCTC ATCGCTTTTT
    GCCAACATCC CTGGTTACAA GCTTGGCTGC TACTTCTGCA ATGATGTGGT GGCCCCAGGA
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGTCGTCC AGGACTGGCC
    GTGATTGCAG GAGCCCTGGC CGTGGAATTG ATGGTATCTG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAT CGGATGAATG AGCCTCCAAC CTCTCTTGGG
    CTTGTGCCTC ACCAGATCCG GGGATTTCTT TCACGGTTTG ATAATGTCCT TCCCGTCAGC
    CTGGCATTTG ACAAATGTAC AGCTTGTTCT TCCAAAGTTC TTGATCAATA TGAACGAGAA
    GGATTTAACT TCCTAGCCAA GGTGTTTAAT TCTTCACATT CCTTCTTAGA AGACTTGACT
    GGTCTTACAT TGCTGCATCA AGAAACCCAA GCTGCTGAGA TCTGGGACAT GAGCGATGAT
    GAGACCATCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu36913
    Clone ID Related Accession (Same CDS sequence) XM_006712932.4 , XM_011533278.2 , XM_006712932.3 , XM_011533277.3 , XM_017005542.1 , XM_006712931.3 , XM_006712931.4 , XM_006712933.3 , XM_011533277.2
    Accession Version XM_006712932.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2217bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product ubiquitin-like modifier-activating enzyme ATG7 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_006712932.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCGGCAG CTACGGGGGA TCCTGGACTC TCTAAACTGC AGTTTGCCCC TTTTAGTAGT 
    GCCTTGGATG TTGGGTTTTG GCATGAGTTG ACCCAGAAGA AGCTGAACGA GTATCGGCTG
    GATGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAATG GTGACTCTGC TGGGCTGCCA
    GCTCGCTTAA CATTGGAGTT CAGTGCTTTT GACATGAGTG CTCCCACCCC AGCCCGTTGC
    TGCCCAGCTA TTGGAACACT GTATAACACC AACACACTCG AGTCTTTCAA GACTGCAGAT
    AAGAAGCTCC TTTTGGAACA AGCAGCAAAT GAGATATGGG AATCCATAAA ATCAGGCACT
    GCTCTTGAAA ACCCTGTACT CCTCAACAAG TTCCTCCTCT TGACATTTGC AGATCTAAAG
    AAGTACCACT TCTACTATTG GTTTTGCTAT CCTGCCCTCT GTCTTCCAGA GAGTTTACCT
    CTCATTCAGG GGCCAGTGGG TTTGGATCAA AGGTTTTCAC TAAAACAGAT TGAAGCACTA
    GAGTGTGCAT ATGATAATCT TTGTCAAACA GAAGGAGTCA CAGCTCTTCC TTACTTCTTA
    ATCAAGTATG ATGAGAACAT GGTGCTGGTT TCCTTGCTTA AACACTACAG TGATTTCTTC
    CAAGGTCAAA GGACGAAGAT AACAATTGGT GTATATGATC CCTGTAACTT AGCCCAGTAC
    CCTGGATGGC CTTTGAGGAA TTTTTTGGTC CTAGCAGCCC ACAGATGGAG TAGCAGTTTC
    CAGTCTGTTG AAGTTGTTTG CTTCCGTGAC CGTACCATGC AGGGGGCGAG AGACGTTGCC
    CACAGCATCA TCTTCGAAGT GAAGCTTCCA GAAATGGCAT TTAGCCCAGA TTGTCCTAAA
    GCAGTTGGAT GGGAAAAGAA CCAGAAAGGA GGCATGGGAC CAAGGATGGT GAACCTCAGT
    GAATGTATGG ACCCTAAAAG GTTAGCTGAG TCATCAGTGG ATCTAAATCT CAAACTGATG
    TGTTGGAGAT TGGTTCCTAC TTTAGACTTG GACAAGGTTG TGTCTGTCAA ATGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGCAATGTA GCTAGGACGT TGATGGGTTG GGGCGTGAGA
    CACATCACAT TTGTGGACAA TGCCAAGATC TCCTACTCCA ATCCTGTGAG GCAGCCTCTC
    TATGAGTTTG AAGATTGCCT AGGGGGTGGT AAGCCCAAGG CTCTGGCAGC AGCGGACCGG
    CTCCAGAAAA TATTCCCCGG TGTGAATGCC AGAGGATTCA ACATGAGCAT ACCTATGCCT
    GGGCATCCAG TGAACTTCTC CAGTGTCACT CTGGAGCAAG CCCGCAGAGA TGTGGAGCAA
    CTGGAGCAGC TCATCGAAAG CCATGATGTC GTCTTCCTAT TGATGGACAC CAGGGAGAGC
    CGGTGGCTTC CTGCCGTCAT TGCTGCAAGC AAGAGAAAGC TGGTCATCAA TGCTGCTTTG
    GGATTTGACA CATTTGTTGT CATGAGACAT GGTCTGAAGA AACCAAAGCA GCAAGGAGCT
    GGGGACTTGT GTCCAAACCA CCCTGTGGCA TCTGCTGACC TCCTGGGCTC ATCGCTTTTT
    GCCAACATCC CTGGTTACAA GCTTGGCTGC TACTTCTGCA ATGATGTGGT GGCCCCAGGA
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGTCGTCC AGGACTGGCC
    GTGATTGCAG GAGCCCTGGC CGTGGAATTG ATGGTATCTG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAT CGGATGAATG AGCCTCCAAC CTCTCTTGGG
    CTTGTGCCTC ACCAGATCCG GGGATTTCTT TCACGGTTTG ATAATGTCCT TCCCGTCAGC
    CTGGCATTTG ACAAATGTAC AGCTTGTTCT TCCAAAGTTC TTGATCAATA TGAACGAGAA
    GGATTTAACT TCCTAGCCAA GGTGTTTAAT TCTTCACATT CCTTCTTAGA AGACTTGACT
    GGTCTTACAT TGCTGCATCA AGAAACCCAA GCTGCTGAGG ATCATCTTTC TTTCATCCGG
    CCCAAGAATG GCACCTTCCC CGCCCATCAG GCCACCTGCA CTCAGGAGTC GGCTGCCTGT
    CCTGAAATAG ATCTCTACCA GCTCTCAAGC AGAACAATTC CTTGCATTCA TACTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006712995.1
    CDS325..2541
    Translation

    Target ORF information:

    RefSeq Version XM_006712932.4
    Organism Homo sapiens(human)
    Definition Homo sapiens autophagy related 7 (ATG7), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006712932.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCGGCAG CTACGGGGGA TCCTGGACTC TCTAAACTGC AGTTTGCCCC TTTTAGTAGT 
    GCCTTGGATG TTGGGTTTTG GCATGAGTTG ACCCAGAAGA AGCTGAACGA GTATCGGCTG
    GATGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAATG GTGACTCTGC TGGGCTGCCA
    GCTCGCTTAA CATTGGAGTT CAGTGCTTTT GACATGAGTG CTCCCACCCC AGCCCGTTGC
    TGCCCAGCTA TTGGAACACT GTATAACACC AACACACTCG AGTCTTTCAA GACTGCAGAT
    AAGAAGCTCC TTTTGGAACA AGCAGCAAAT GAGATATGGG AATCCATAAA ATCAGGCACT
    GCTCTTGAAA ACCCTGTACT CCTCAACAAG TTCCTCCTCT TGACATTTGC AGATCTAAAG
    AAGTACCACT TCTACTATTG GTTTTGCTAT CCTGCCCTCT GTCTTCCAGA GAGTTTACCT
    CTCATTCAGG GGCCAGTGGG TTTGGATCAA AGGTTTTCAC TAAAACAGAT TGAAGCACTA
    GAGTGTGCAT ATGATAATCT TTGTCAAACA GAAGGAGTCA CAGCTCTTCC TTACTTCTTA
    ATCAAGTATG ATGAGAACAT GGTGCTGGTT TCCTTGCTTA AACACTACAG TGATTTCTTC
    CAAGGTCAAA GGACGAAGAT AACAATTGGT GTATATGATC CCTGTAACTT AGCCCAGTAC
    CCTGGATGGC CTTTGAGGAA TTTTTTGGTC CTAGCAGCCC ACAGATGGAG TAGCAGTTTC
    CAGTCTGTTG AAGTTGTTTG CTTCCGTGAC CGTACCATGC AGGGGGCGAG AGACGTTGCC
    CACAGCATCA TCTTCGAAGT GAAGCTTCCA GAAATGGCAT TTAGCCCAGA TTGTCCTAAA
    GCAGTTGGAT GGGAAAAGAA CCAGAAAGGA GGCATGGGAC CAAGGATGGT GAACCTCAGT
    GAATGTATGG ACCCTAAAAG GTTAGCTGAG TCATCAGTGG ATCTAAATCT CAAACTGATG
    TGTTGGAGAT TGGTTCCTAC TTTAGACTTG GACAAGGTTG TGTCTGTCAA ATGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGCAATGTA GCTAGGACGT TGATGGGTTG GGGCGTGAGA
    CACATCACAT TTGTGGACAA TGCCAAGATC TCCTACTCCA ATCCTGTGAG GCAGCCTCTC
    TATGAGTTTG AAGATTGCCT AGGGGGTGGT AAGCCCAAGG CTCTGGCAGC AGCGGACCGG
    CTCCAGAAAA TATTCCCCGG TGTGAATGCC AGAGGATTCA ACATGAGCAT ACCTATGCCT
    GGGCATCCAG TGAACTTCTC CAGTGTCACT CTGGAGCAAG CCCGCAGAGA TGTGGAGCAA
    CTGGAGCAGC TCATCGAAAG CCATGATGTC GTCTTCCTAT TGATGGACAC CAGGGAGAGC
    CGGTGGCTTC CTGCCGTCAT TGCTGCAAGC AAGAGAAAGC TGGTCATCAA TGCTGCTTTG
    GGATTTGACA CATTTGTTGT CATGAGACAT GGTCTGAAGA AACCAAAGCA GCAAGGAGCT
    GGGGACTTGT GTCCAAACCA CCCTGTGGCA TCTGCTGACC TCCTGGGCTC ATCGCTTTTT
    GCCAACATCC CTGGTTACAA GCTTGGCTGC TACTTCTGCA ATGATGTGGT GGCCCCAGGA
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGTCGTCC AGGACTGGCC
    GTGATTGCAG GAGCCCTGGC CGTGGAATTG ATGGTATCTG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAT CGGATGAATG AGCCTCCAAC CTCTCTTGGG
    CTTGTGCCTC ACCAGATCCG GGGATTTCTT TCACGGTTTG ATAATGTCCT TCCCGTCAGC
    CTGGCATTTG ACAAATGTAC AGCTTGTTCT TCCAAAGTTC TTGATCAATA TGAACGAGAA
    GGATTTAACT TCCTAGCCAA GGTGTTTAAT TCTTCACATT CCTTCTTAGA AGACTTGACT
    GGTCTTACAT TGCTGCATCA AGAAACCCAA GCTGCTGAGG ATCATCTTTC TTTCATCCGG
    CCCAAGAATG GCACCTTCCC CGCCCATCAG GCCACCTGCA CTCAGGAGTC GGCTGCCTGT
    CCTGAAATAG ATCTCTACCA GCTCTCAAGC AGAACAATTC CTTGCATTCA TACTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu36913
    Clone ID Related Accession (Same CDS sequence) XM_006712932.4 , XM_011533278.2 , XM_006712932.3 , XM_011533277.3 , XM_017005542.1 , XM_006712931.3 , XM_006712931.4 , XM_006712933.3 , XM_011533277.2
    Accession Version XM_011533278.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2217bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product ubiquitin-like modifier-activating enzyme ATG7 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011533278.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCGGCAG CTACGGGGGA TCCTGGACTC TCTAAACTGC AGTTTGCCCC TTTTAGTAGT 
    GCCTTGGATG TTGGGTTTTG GCATGAGTTG ACCCAGAAGA AGCTGAACGA GTATCGGCTG
    GATGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAATG GTGACTCTGC TGGGCTGCCA
    GCTCGCTTAA CATTGGAGTT CAGTGCTTTT GACATGAGTG CTCCCACCCC AGCCCGTTGC
    TGCCCAGCTA TTGGAACACT GTATAACACC AACACACTCG AGTCTTTCAA GACTGCAGAT
    AAGAAGCTCC TTTTGGAACA AGCAGCAAAT GAGATATGGG AATCCATAAA ATCAGGCACT
    GCTCTTGAAA ACCCTGTACT CCTCAACAAG TTCCTCCTCT TGACATTTGC AGATCTAAAG
    AAGTACCACT TCTACTATTG GTTTTGCTAT CCTGCCCTCT GTCTTCCAGA GAGTTTACCT
    CTCATTCAGG GGCCAGTGGG TTTGGATCAA AGGTTTTCAC TAAAACAGAT TGAAGCACTA
    GAGTGTGCAT ATGATAATCT TTGTCAAACA GAAGGAGTCA CAGCTCTTCC TTACTTCTTA
    ATCAAGTATG ATGAGAACAT GGTGCTGGTT TCCTTGCTTA AACACTACAG TGATTTCTTC
    CAAGGTCAAA GGACGAAGAT AACAATTGGT GTATATGATC CCTGTAACTT AGCCCAGTAC
    CCTGGATGGC CTTTGAGGAA TTTTTTGGTC CTAGCAGCCC ACAGATGGAG TAGCAGTTTC
    CAGTCTGTTG AAGTTGTTTG CTTCCGTGAC CGTACCATGC AGGGGGCGAG AGACGTTGCC
    CACAGCATCA TCTTCGAAGT GAAGCTTCCA GAAATGGCAT TTAGCCCAGA TTGTCCTAAA
    GCAGTTGGAT GGGAAAAGAA CCAGAAAGGA GGCATGGGAC CAAGGATGGT GAACCTCAGT
    GAATGTATGG ACCCTAAAAG GTTAGCTGAG TCATCAGTGG ATCTAAATCT CAAACTGATG
    TGTTGGAGAT TGGTTCCTAC TTTAGACTTG GACAAGGTTG TGTCTGTCAA ATGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGCAATGTA GCTAGGACGT TGATGGGTTG GGGCGTGAGA
    CACATCACAT TTGTGGACAA TGCCAAGATC TCCTACTCCA ATCCTGTGAG GCAGCCTCTC
    TATGAGTTTG AAGATTGCCT AGGGGGTGGT AAGCCCAAGG CTCTGGCAGC AGCGGACCGG
    CTCCAGAAAA TATTCCCCGG TGTGAATGCC AGAGGATTCA ACATGAGCAT ACCTATGCCT
    GGGCATCCAG TGAACTTCTC CAGTGTCACT CTGGAGCAAG CCCGCAGAGA TGTGGAGCAA
    CTGGAGCAGC TCATCGAAAG CCATGATGTC GTCTTCCTAT TGATGGACAC CAGGGAGAGC
    CGGTGGCTTC CTGCCGTCAT TGCTGCAAGC AAGAGAAAGC TGGTCATCAA TGCTGCTTTG
    GGATTTGACA CATTTGTTGT CATGAGACAT GGTCTGAAGA AACCAAAGCA GCAAGGAGCT
    GGGGACTTGT GTCCAAACCA CCCTGTGGCA TCTGCTGACC TCCTGGGCTC ATCGCTTTTT
    GCCAACATCC CTGGTTACAA GCTTGGCTGC TACTTCTGCA ATGATGTGGT GGCCCCAGGA
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGTCGTCC AGGACTGGCC
    GTGATTGCAG GAGCCCTGGC CGTGGAATTG ATGGTATCTG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAT CGGATGAATG AGCCTCCAAC CTCTCTTGGG
    CTTGTGCCTC ACCAGATCCG GGGATTTCTT TCACGGTTTG ATAATGTCCT TCCCGTCAGC
    CTGGCATTTG ACAAATGTAC AGCTTGTTCT TCCAAAGTTC TTGATCAATA TGAACGAGAA
    GGATTTAACT TCCTAGCCAA GGTGTTTAAT TCTTCACATT CCTTCTTAGA AGACTTGACT
    GGTCTTACAT TGCTGCATCA AGAAACCCAA GCTGCTGAGG ATCATCTTTC TTTCATCCGG
    CCCAAGAATG GCACCTTCCC CGCCCATCAG GCCACCTGCA CTCAGGAGTC GGCTGCCTGT
    CCTGAAATAG ATCTCTACCA GCTCTCAAGC AGAACAATTC CTTGCATTCA TACTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011531580.1
    CDS154..2370
    Translation

    Target ORF information:

    RefSeq Version XM_011533278.2
    Organism Homo sapiens(human)
    Definition Homo sapiens autophagy related 7 (ATG7), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011533278.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCGGCAG CTACGGGGGA TCCTGGACTC TCTAAACTGC AGTTTGCCCC TTTTAGTAGT 
    GCCTTGGATG TTGGGTTTTG GCATGAGTTG ACCCAGAAGA AGCTGAACGA GTATCGGCTG
    GATGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAATG GTGACTCTGC TGGGCTGCCA
    GCTCGCTTAA CATTGGAGTT CAGTGCTTTT GACATGAGTG CTCCCACCCC AGCCCGTTGC
    TGCCCAGCTA TTGGAACACT GTATAACACC AACACACTCG AGTCTTTCAA GACTGCAGAT
    AAGAAGCTCC TTTTGGAACA AGCAGCAAAT GAGATATGGG AATCCATAAA ATCAGGCACT
    GCTCTTGAAA ACCCTGTACT CCTCAACAAG TTCCTCCTCT TGACATTTGC AGATCTAAAG
    AAGTACCACT TCTACTATTG GTTTTGCTAT CCTGCCCTCT GTCTTCCAGA GAGTTTACCT
    CTCATTCAGG GGCCAGTGGG TTTGGATCAA AGGTTTTCAC TAAAACAGAT TGAAGCACTA
    GAGTGTGCAT ATGATAATCT TTGTCAAACA GAAGGAGTCA CAGCTCTTCC TTACTTCTTA
    ATCAAGTATG ATGAGAACAT GGTGCTGGTT TCCTTGCTTA AACACTACAG TGATTTCTTC
    CAAGGTCAAA GGACGAAGAT AACAATTGGT GTATATGATC CCTGTAACTT AGCCCAGTAC
    CCTGGATGGC CTTTGAGGAA TTTTTTGGTC CTAGCAGCCC ACAGATGGAG TAGCAGTTTC
    CAGTCTGTTG AAGTTGTTTG CTTCCGTGAC CGTACCATGC AGGGGGCGAG AGACGTTGCC
    CACAGCATCA TCTTCGAAGT GAAGCTTCCA GAAATGGCAT TTAGCCCAGA TTGTCCTAAA
    GCAGTTGGAT GGGAAAAGAA CCAGAAAGGA GGCATGGGAC CAAGGATGGT GAACCTCAGT
    GAATGTATGG ACCCTAAAAG GTTAGCTGAG TCATCAGTGG ATCTAAATCT CAAACTGATG
    TGTTGGAGAT TGGTTCCTAC TTTAGACTTG GACAAGGTTG TGTCTGTCAA ATGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGCAATGTA GCTAGGACGT TGATGGGTTG GGGCGTGAGA
    CACATCACAT TTGTGGACAA TGCCAAGATC TCCTACTCCA ATCCTGTGAG GCAGCCTCTC
    TATGAGTTTG AAGATTGCCT AGGGGGTGGT AAGCCCAAGG CTCTGGCAGC AGCGGACCGG
    CTCCAGAAAA TATTCCCCGG TGTGAATGCC AGAGGATTCA ACATGAGCAT ACCTATGCCT
    GGGCATCCAG TGAACTTCTC CAGTGTCACT CTGGAGCAAG CCCGCAGAGA TGTGGAGCAA
    CTGGAGCAGC TCATCGAAAG CCATGATGTC GTCTTCCTAT TGATGGACAC CAGGGAGAGC
    CGGTGGCTTC CTGCCGTCAT TGCTGCAAGC AAGAGAAAGC TGGTCATCAA TGCTGCTTTG
    GGATTTGACA CATTTGTTGT CATGAGACAT GGTCTGAAGA AACCAAAGCA GCAAGGAGCT
    GGGGACTTGT GTCCAAACCA CCCTGTGGCA TCTGCTGACC TCCTGGGCTC ATCGCTTTTT
    GCCAACATCC CTGGTTACAA GCTTGGCTGC TACTTCTGCA ATGATGTGGT GGCCCCAGGA
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGTCGTCC AGGACTGGCC
    GTGATTGCAG GAGCCCTGGC CGTGGAATTG ATGGTATCTG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAT CGGATGAATG AGCCTCCAAC CTCTCTTGGG
    CTTGTGCCTC ACCAGATCCG GGGATTTCTT TCACGGTTTG ATAATGTCCT TCCCGTCAGC
    CTGGCATTTG ACAAATGTAC AGCTTGTTCT TCCAAAGTTC TTGATCAATA TGAACGAGAA
    GGATTTAACT TCCTAGCCAA GGTGTTTAAT TCTTCACATT CCTTCTTAGA AGACTTGACT
    GGTCTTACAT TGCTGCATCA AGAAACCCAA GCTGCTGAGG ATCATCTTTC TTTCATCCGG
    CCCAAGAATG GCACCTTCCC CGCCCATCAG GCCACCTGCA CTCAGGAGTC GGCTGCCTGT
    CCTGAAATAG ATCTCTACCA GCTCTCAAGC AGAACAATTC CTTGCATTCA TACTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu36913
    Clone ID Related Accession (Same CDS sequence) XM_006712932.4 , XM_011533278.2 , XM_006712932.3 , XM_011533277.3 , XM_017005542.1 , XM_006712931.3 , XM_006712931.4 , XM_006712933.3 , XM_011533277.2
    Accession Version XM_006712932.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2217bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product ubiquitin-like modifier-activating enzyme ATG7 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006712932.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCGGCAG CTACGGGGGA TCCTGGACTC TCTAAACTGC AGTTTGCCCC TTTTAGTAGT 
    GCCTTGGATG TTGGGTTTTG GCATGAGTTG ACCCAGAAGA AGCTGAACGA GTATCGGCTG
    GATGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAATG GTGACTCTGC TGGGCTGCCA
    GCTCGCTTAA CATTGGAGTT CAGTGCTTTT GACATGAGTG CTCCCACCCC AGCCCGTTGC
    TGCCCAGCTA TTGGAACACT GTATAACACC AACACACTCG AGTCTTTCAA GACTGCAGAT
    AAGAAGCTCC TTTTGGAACA AGCAGCAAAT GAGATATGGG AATCCATAAA ATCAGGCACT
    GCTCTTGAAA ACCCTGTACT CCTCAACAAG TTCCTCCTCT TGACATTTGC AGATCTAAAG
    AAGTACCACT TCTACTATTG GTTTTGCTAT CCTGCCCTCT GTCTTCCAGA GAGTTTACCT
    CTCATTCAGG GGCCAGTGGG TTTGGATCAA AGGTTTTCAC TAAAACAGAT TGAAGCACTA
    GAGTGTGCAT ATGATAATCT TTGTCAAACA GAAGGAGTCA CAGCTCTTCC TTACTTCTTA
    ATCAAGTATG ATGAGAACAT GGTGCTGGTT TCCTTGCTTA AACACTACAG TGATTTCTTC
    CAAGGTCAAA GGACGAAGAT AACAATTGGT GTATATGATC CCTGTAACTT AGCCCAGTAC
    CCTGGATGGC CTTTGAGGAA TTTTTTGGTC CTAGCAGCCC ACAGATGGAG TAGCAGTTTC
    CAGTCTGTTG AAGTTGTTTG CTTCCGTGAC CGTACCATGC AGGGGGCGAG AGACGTTGCC
    CACAGCATCA TCTTCGAAGT GAAGCTTCCA GAAATGGCAT TTAGCCCAGA TTGTCCTAAA
    GCAGTTGGAT GGGAAAAGAA CCAGAAAGGA GGCATGGGAC CAAGGATGGT GAACCTCAGT
    GAATGTATGG ACCCTAAAAG GTTAGCTGAG TCATCAGTGG ATCTAAATCT CAAACTGATG
    TGTTGGAGAT TGGTTCCTAC TTTAGACTTG GACAAGGTTG TGTCTGTCAA ATGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGCAATGTA GCTAGGACGT TGATGGGTTG GGGCGTGAGA
    CACATCACAT TTGTGGACAA TGCCAAGATC TCCTACTCCA ATCCTGTGAG GCAGCCTCTC
    TATGAGTTTG AAGATTGCCT AGGGGGTGGT AAGCCCAAGG CTCTGGCAGC AGCGGACCGG
    CTCCAGAAAA TATTCCCCGG TGTGAATGCC AGAGGATTCA ACATGAGCAT ACCTATGCCT
    GGGCATCCAG TGAACTTCTC CAGTGTCACT CTGGAGCAAG CCCGCAGAGA TGTGGAGCAA
    CTGGAGCAGC TCATCGAAAG CCATGATGTC GTCTTCCTAT TGATGGACAC CAGGGAGAGC
    CGGTGGCTTC CTGCCGTCAT TGCTGCAAGC AAGAGAAAGC TGGTCATCAA TGCTGCTTTG
    GGATTTGACA CATTTGTTGT CATGAGACAT GGTCTGAAGA AACCAAAGCA GCAAGGAGCT
    GGGGACTTGT GTCCAAACCA CCCTGTGGCA TCTGCTGACC TCCTGGGCTC ATCGCTTTTT
    GCCAACATCC CTGGTTACAA GCTTGGCTGC TACTTCTGCA ATGATGTGGT GGCCCCAGGA
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGTCGTCC AGGACTGGCC
    GTGATTGCAG GAGCCCTGGC CGTGGAATTG ATGGTATCTG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAT CGGATGAATG AGCCTCCAAC CTCTCTTGGG
    CTTGTGCCTC ACCAGATCCG GGGATTTCTT TCACGGTTTG ATAATGTCCT TCCCGTCAGC
    CTGGCATTTG ACAAATGTAC AGCTTGTTCT TCCAAAGTTC TTGATCAATA TGAACGAGAA
    GGATTTAACT TCCTAGCCAA GGTGTTTAAT TCTTCACATT CCTTCTTAGA AGACTTGACT
    GGTCTTACAT TGCTGCATCA AGAAACCCAA GCTGCTGAGG ATCATCTTTC TTTCATCCGG
    CCCAAGAATG GCACCTTCCC CGCCCATCAG GCCACCTGCA CTCAGGAGTC GGCTGCCTGT
    CCTGAAATAG ATCTCTACCA GCTCTCAAGC AGAACAATTC CTTGCATTCA TACTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006712995.1
    CDS325..2541
    Translation

    Target ORF information:

    RefSeq Version XM_006712932.3
    Organism Homo sapiens(human)
    Definition Homo sapiens autophagy related 7 (ATG7), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006712932.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCGGCAG CTACGGGGGA TCCTGGACTC TCTAAACTGC AGTTTGCCCC TTTTAGTAGT 
    GCCTTGGATG TTGGGTTTTG GCATGAGTTG ACCCAGAAGA AGCTGAACGA GTATCGGCTG
    GATGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAATG GTGACTCTGC TGGGCTGCCA
    GCTCGCTTAA CATTGGAGTT CAGTGCTTTT GACATGAGTG CTCCCACCCC AGCCCGTTGC
    TGCCCAGCTA TTGGAACACT GTATAACACC AACACACTCG AGTCTTTCAA GACTGCAGAT
    AAGAAGCTCC TTTTGGAACA AGCAGCAAAT GAGATATGGG AATCCATAAA ATCAGGCACT
    GCTCTTGAAA ACCCTGTACT CCTCAACAAG TTCCTCCTCT TGACATTTGC AGATCTAAAG
    AAGTACCACT TCTACTATTG GTTTTGCTAT CCTGCCCTCT GTCTTCCAGA GAGTTTACCT
    CTCATTCAGG GGCCAGTGGG TTTGGATCAA AGGTTTTCAC TAAAACAGAT TGAAGCACTA
    GAGTGTGCAT ATGATAATCT TTGTCAAACA GAAGGAGTCA CAGCTCTTCC TTACTTCTTA
    ATCAAGTATG ATGAGAACAT GGTGCTGGTT TCCTTGCTTA AACACTACAG TGATTTCTTC
    CAAGGTCAAA GGACGAAGAT AACAATTGGT GTATATGATC CCTGTAACTT AGCCCAGTAC
    CCTGGATGGC CTTTGAGGAA TTTTTTGGTC CTAGCAGCCC ACAGATGGAG TAGCAGTTTC
    CAGTCTGTTG AAGTTGTTTG CTTCCGTGAC CGTACCATGC AGGGGGCGAG AGACGTTGCC
    CACAGCATCA TCTTCGAAGT GAAGCTTCCA GAAATGGCAT TTAGCCCAGA TTGTCCTAAA
    GCAGTTGGAT GGGAAAAGAA CCAGAAAGGA GGCATGGGAC CAAGGATGGT GAACCTCAGT
    GAATGTATGG ACCCTAAAAG GTTAGCTGAG TCATCAGTGG ATCTAAATCT CAAACTGATG
    TGTTGGAGAT TGGTTCCTAC TTTAGACTTG GACAAGGTTG TGTCTGTCAA ATGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGCAATGTA GCTAGGACGT TGATGGGTTG GGGCGTGAGA
    CACATCACAT TTGTGGACAA TGCCAAGATC TCCTACTCCA ATCCTGTGAG GCAGCCTCTC
    TATGAGTTTG AAGATTGCCT AGGGGGTGGT AAGCCCAAGG CTCTGGCAGC AGCGGACCGG
    CTCCAGAAAA TATTCCCCGG TGTGAATGCC AGAGGATTCA ACATGAGCAT ACCTATGCCT
    GGGCATCCAG TGAACTTCTC CAGTGTCACT CTGGAGCAAG CCCGCAGAGA TGTGGAGCAA
    CTGGAGCAGC TCATCGAAAG CCATGATGTC GTCTTCCTAT TGATGGACAC CAGGGAGAGC
    CGGTGGCTTC CTGCCGTCAT TGCTGCAAGC AAGAGAAAGC TGGTCATCAA TGCTGCTTTG
    GGATTTGACA CATTTGTTGT CATGAGACAT GGTCTGAAGA AACCAAAGCA GCAAGGAGCT
    GGGGACTTGT GTCCAAACCA CCCTGTGGCA TCTGCTGACC TCCTGGGCTC ATCGCTTTTT
    GCCAACATCC CTGGTTACAA GCTTGGCTGC TACTTCTGCA ATGATGTGGT GGCCCCAGGA
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGTCGTCC AGGACTGGCC
    GTGATTGCAG GAGCCCTGGC CGTGGAATTG ATGGTATCTG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAT CGGATGAATG AGCCTCCAAC CTCTCTTGGG
    CTTGTGCCTC ACCAGATCCG GGGATTTCTT TCACGGTTTG ATAATGTCCT TCCCGTCAGC
    CTGGCATTTG ACAAATGTAC AGCTTGTTCT TCCAAAGTTC TTGATCAATA TGAACGAGAA
    GGATTTAACT TCCTAGCCAA GGTGTTTAAT TCTTCACATT CCTTCTTAGA AGACTTGACT
    GGTCTTACAT TGCTGCATCA AGAAACCCAA GCTGCTGAGG ATCATCTTTC TTTCATCCGG
    CCCAAGAATG GCACCTTCCC CGCCCATCAG GCCACCTGCA CTCAGGAGTC GGCTGCCTGT
    CCTGAAATAG ATCTCTACCA GCTCTCAAGC AGAACAATTC CTTGCATTCA TACTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu36913
    Clone ID Related Accession (Same CDS sequence) XM_006712932.4 , XM_011533278.2 , XM_006712932.3 , XM_011533277.3 , XM_017005542.1 , XM_006712931.3 , XM_006712931.4 , XM_006712933.3 , XM_011533277.2
    Accession Version XM_011533277.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2217bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product ubiquitin-like modifier-activating enzyme ATG7 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011533277.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCGGCAG CTACGGGGGA TCCTGGACTC TCTAAACTGC AGTTTGCCCC TTTTAGTAGT 
    GCCTTGGATG TTGGGTTTTG GCATGAGTTG ACCCAGAAGA AGCTGAACGA GTATCGGCTG
    GATGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAATG GTGACTCTGC TGGGCTGCCA
    GCTCGCTTAA CATTGGAGTT CAGTGCTTTT GACATGAGTG CTCCCACCCC AGCCCGTTGC
    TGCCCAGCTA TTGGAACACT GTATAACACC AACACACTCG AGTCTTTCAA GACTGCAGAT
    AAGAAGCTCC TTTTGGAACA AGCAGCAAAT GAGATATGGG AATCCATAAA ATCAGGCACT
    GCTCTTGAAA ACCCTGTACT CCTCAACAAG TTCCTCCTCT TGACATTTGC AGATCTAAAG
    AAGTACCACT TCTACTATTG GTTTTGCTAT CCTGCCCTCT GTCTTCCAGA GAGTTTACCT
    CTCATTCAGG GGCCAGTGGG TTTGGATCAA AGGTTTTCAC TAAAACAGAT TGAAGCACTA
    GAGTGTGCAT ATGATAATCT TTGTCAAACA GAAGGAGTCA CAGCTCTTCC TTACTTCTTA
    ATCAAGTATG ATGAGAACAT GGTGCTGGTT TCCTTGCTTA AACACTACAG TGATTTCTTC
    CAAGGTCAAA GGACGAAGAT AACAATTGGT GTATATGATC CCTGTAACTT AGCCCAGTAC
    CCTGGATGGC CTTTGAGGAA TTTTTTGGTC CTAGCAGCCC ACAGATGGAG TAGCAGTTTC
    CAGTCTGTTG AAGTTGTTTG CTTCCGTGAC CGTACCATGC AGGGGGCGAG AGACGTTGCC
    CACAGCATCA TCTTCGAAGT GAAGCTTCCA GAAATGGCAT TTAGCCCAGA TTGTCCTAAA
    GCAGTTGGAT GGGAAAAGAA CCAGAAAGGA GGCATGGGAC CAAGGATGGT GAACCTCAGT
    GAATGTATGG ACCCTAAAAG GTTAGCTGAG TCATCAGTGG ATCTAAATCT CAAACTGATG
    TGTTGGAGAT TGGTTCCTAC TTTAGACTTG GACAAGGTTG TGTCTGTCAA ATGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGCAATGTA GCTAGGACGT TGATGGGTTG GGGCGTGAGA
    CACATCACAT TTGTGGACAA TGCCAAGATC TCCTACTCCA ATCCTGTGAG GCAGCCTCTC
    TATGAGTTTG AAGATTGCCT AGGGGGTGGT AAGCCCAAGG CTCTGGCAGC AGCGGACCGG
    CTCCAGAAAA TATTCCCCGG TGTGAATGCC AGAGGATTCA ACATGAGCAT ACCTATGCCT
    GGGCATCCAG TGAACTTCTC CAGTGTCACT CTGGAGCAAG CCCGCAGAGA TGTGGAGCAA
    CTGGAGCAGC TCATCGAAAG CCATGATGTC GTCTTCCTAT TGATGGACAC CAGGGAGAGC
    CGGTGGCTTC CTGCCGTCAT TGCTGCAAGC AAGAGAAAGC TGGTCATCAA TGCTGCTTTG
    GGATTTGACA CATTTGTTGT CATGAGACAT GGTCTGAAGA AACCAAAGCA GCAAGGAGCT
    GGGGACTTGT GTCCAAACCA CCCTGTGGCA TCTGCTGACC TCCTGGGCTC ATCGCTTTTT
    GCCAACATCC CTGGTTACAA GCTTGGCTGC TACTTCTGCA ATGATGTGGT GGCCCCAGGA
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGTCGTCC AGGACTGGCC
    GTGATTGCAG GAGCCCTGGC CGTGGAATTG ATGGTATCTG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAT CGGATGAATG AGCCTCCAAC CTCTCTTGGG
    CTTGTGCCTC ACCAGATCCG GGGATTTCTT TCACGGTTTG ATAATGTCCT TCCCGTCAGC
    CTGGCATTTG ACAAATGTAC AGCTTGTTCT TCCAAAGTTC TTGATCAATA TGAACGAGAA
    GGATTTAACT TCCTAGCCAA GGTGTTTAAT TCTTCACATT CCTTCTTAGA AGACTTGACT
    GGTCTTACAT TGCTGCATCA AGAAACCCAA GCTGCTGAGG ATCATCTTTC TTTCATCCGG
    CCCAAGAATG GCACCTTCCC CGCCCATCAG GCCACCTGCA CTCAGGAGTC GGCTGCCTGT
    CCTGAAATAG ATCTCTACCA GCTCTCAAGC AGAACAATTC CTTGCATTCA TACTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011531579.1
    CDS449..2665
    Translation

    Target ORF information:

    RefSeq Version XM_011533277.3
    Organism Homo sapiens(human)
    Definition Homo sapiens autophagy related 7 (ATG7), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011533277.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCGGCAG CTACGGGGGA TCCTGGACTC TCTAAACTGC AGTTTGCCCC TTTTAGTAGT 
    GCCTTGGATG TTGGGTTTTG GCATGAGTTG ACCCAGAAGA AGCTGAACGA GTATCGGCTG
    GATGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAATG GTGACTCTGC TGGGCTGCCA
    GCTCGCTTAA CATTGGAGTT CAGTGCTTTT GACATGAGTG CTCCCACCCC AGCCCGTTGC
    TGCCCAGCTA TTGGAACACT GTATAACACC AACACACTCG AGTCTTTCAA GACTGCAGAT
    AAGAAGCTCC TTTTGGAACA AGCAGCAAAT GAGATATGGG AATCCATAAA ATCAGGCACT
    GCTCTTGAAA ACCCTGTACT CCTCAACAAG TTCCTCCTCT TGACATTTGC AGATCTAAAG
    AAGTACCACT TCTACTATTG GTTTTGCTAT CCTGCCCTCT GTCTTCCAGA GAGTTTACCT
    CTCATTCAGG GGCCAGTGGG TTTGGATCAA AGGTTTTCAC TAAAACAGAT TGAAGCACTA
    GAGTGTGCAT ATGATAATCT TTGTCAAACA GAAGGAGTCA CAGCTCTTCC TTACTTCTTA
    ATCAAGTATG ATGAGAACAT GGTGCTGGTT TCCTTGCTTA AACACTACAG TGATTTCTTC
    CAAGGTCAAA GGACGAAGAT AACAATTGGT GTATATGATC CCTGTAACTT AGCCCAGTAC
    CCTGGATGGC CTTTGAGGAA TTTTTTGGTC CTAGCAGCCC ACAGATGGAG TAGCAGTTTC
    CAGTCTGTTG AAGTTGTTTG CTTCCGTGAC CGTACCATGC AGGGGGCGAG AGACGTTGCC
    CACAGCATCA TCTTCGAAGT GAAGCTTCCA GAAATGGCAT TTAGCCCAGA TTGTCCTAAA
    GCAGTTGGAT GGGAAAAGAA CCAGAAAGGA GGCATGGGAC CAAGGATGGT GAACCTCAGT
    GAATGTATGG ACCCTAAAAG GTTAGCTGAG TCATCAGTGG ATCTAAATCT CAAACTGATG
    TGTTGGAGAT TGGTTCCTAC TTTAGACTTG GACAAGGTTG TGTCTGTCAA ATGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGCAATGTA GCTAGGACGT TGATGGGTTG GGGCGTGAGA
    CACATCACAT TTGTGGACAA TGCCAAGATC TCCTACTCCA ATCCTGTGAG GCAGCCTCTC
    TATGAGTTTG AAGATTGCCT AGGGGGTGGT AAGCCCAAGG CTCTGGCAGC AGCGGACCGG
    CTCCAGAAAA TATTCCCCGG TGTGAATGCC AGAGGATTCA ACATGAGCAT ACCTATGCCT
    GGGCATCCAG TGAACTTCTC CAGTGTCACT CTGGAGCAAG CCCGCAGAGA TGTGGAGCAA
    CTGGAGCAGC TCATCGAAAG CCATGATGTC GTCTTCCTAT TGATGGACAC CAGGGAGAGC
    CGGTGGCTTC CTGCCGTCAT TGCTGCAAGC AAGAGAAAGC TGGTCATCAA TGCTGCTTTG
    GGATTTGACA CATTTGTTGT CATGAGACAT GGTCTGAAGA AACCAAAGCA GCAAGGAGCT
    GGGGACTTGT GTCCAAACCA CCCTGTGGCA TCTGCTGACC TCCTGGGCTC ATCGCTTTTT
    GCCAACATCC CTGGTTACAA GCTTGGCTGC TACTTCTGCA ATGATGTGGT GGCCCCAGGA
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGTCGTCC AGGACTGGCC
    GTGATTGCAG GAGCCCTGGC CGTGGAATTG ATGGTATCTG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAT CGGATGAATG AGCCTCCAAC CTCTCTTGGG
    CTTGTGCCTC ACCAGATCCG GGGATTTCTT TCACGGTTTG ATAATGTCCT TCCCGTCAGC
    CTGGCATTTG ACAAATGTAC AGCTTGTTCT TCCAAAGTTC TTGATCAATA TGAACGAGAA
    GGATTTAACT TCCTAGCCAA GGTGTTTAAT TCTTCACATT CCTTCTTAGA AGACTTGACT
    GGTCTTACAT TGCTGCATCA AGAAACCCAA GCTGCTGAGG ATCATCTTTC TTTCATCCGG
    CCCAAGAATG GCACCTTCCC CGCCCATCAG GCCACCTGCA CTCAGGAGTC GGCTGCCTGT
    CCTGAAATAG ATCTCTACCA GCTCTCAAGC AGAACAATTC CTTGCATTCA TACTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu36913
    Clone ID Related Accession (Same CDS sequence) XM_006712932.4 , XM_011533278.2 , XM_006712932.3 , XM_011533277.3 , XM_017005542.1 , XM_006712931.3 , XM_006712931.4 , XM_006712933.3 , XM_011533277.2
    Accession Version XM_017005542.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2217bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product ubiquitin-like modifier-activating enzyme ATG7 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCGGCAG CTACGGGGGA TCCTGGACTC TCTAAACTGC AGTTTGCCCC TTTTAGTAGT 
    GCCTTGGATG TTGGGTTTTG GCATGAGTTG ACCCAGAAGA AGCTGAACGA GTATCGGCTG
    GATGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAATG GTGACTCTGC TGGGCTGCCA
    GCTCGCTTAA CATTGGAGTT CAGTGCTTTT GACATGAGTG CTCCCACCCC AGCCCGTTGC
    TGCCCAGCTA TTGGAACACT GTATAACACC AACACACTCG AGTCTTTCAA GACTGCAGAT
    AAGAAGCTCC TTTTGGAACA AGCAGCAAAT GAGATATGGG AATCCATAAA ATCAGGCACT
    GCTCTTGAAA ACCCTGTACT CCTCAACAAG TTCCTCCTCT TGACATTTGC AGATCTAAAG
    AAGTACCACT TCTACTATTG GTTTTGCTAT CCTGCCCTCT GTCTTCCAGA GAGTTTACCT
    CTCATTCAGG GGCCAGTGGG TTTGGATCAA AGGTTTTCAC TAAAACAGAT TGAAGCACTA
    GAGTGTGCAT ATGATAATCT TTGTCAAACA GAAGGAGTCA CAGCTCTTCC TTACTTCTTA
    ATCAAGTATG ATGAGAACAT GGTGCTGGTT TCCTTGCTTA AACACTACAG TGATTTCTTC
    CAAGGTCAAA GGACGAAGAT AACAATTGGT GTATATGATC CCTGTAACTT AGCCCAGTAC
    CCTGGATGGC CTTTGAGGAA TTTTTTGGTC CTAGCAGCCC ACAGATGGAG TAGCAGTTTC
    CAGTCTGTTG AAGTTGTTTG CTTCCGTGAC CGTACCATGC AGGGGGCGAG AGACGTTGCC
    CACAGCATCA TCTTCGAAGT GAAGCTTCCA GAAATGGCAT TTAGCCCAGA TTGTCCTAAA
    GCAGTTGGAT GGGAAAAGAA CCAGAAAGGA GGCATGGGAC CAAGGATGGT GAACCTCAGT
    GAATGTATGG ACCCTAAAAG GTTAGCTGAG TCATCAGTGG ATCTAAATCT CAAACTGATG
    TGTTGGAGAT TGGTTCCTAC TTTAGACTTG GACAAGGTTG TGTCTGTCAA ATGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGCAATGTA GCTAGGACGT TGATGGGTTG GGGCGTGAGA
    CACATCACAT TTGTGGACAA TGCCAAGATC TCCTACTCCA ATCCTGTGAG GCAGCCTCTC
    TATGAGTTTG AAGATTGCCT AGGGGGTGGT AAGCCCAAGG CTCTGGCAGC AGCGGACCGG
    CTCCAGAAAA TATTCCCCGG TGTGAATGCC AGAGGATTCA ACATGAGCAT ACCTATGCCT
    GGGCATCCAG TGAACTTCTC CAGTGTCACT CTGGAGCAAG CCCGCAGAGA TGTGGAGCAA
    CTGGAGCAGC TCATCGAAAG CCATGATGTC GTCTTCCTAT TGATGGACAC CAGGGAGAGC
    CGGTGGCTTC CTGCCGTCAT TGCTGCAAGC AAGAGAAAGC TGGTCATCAA TGCTGCTTTG
    GGATTTGACA CATTTGTTGT CATGAGACAT GGTCTGAAGA AACCAAAGCA GCAAGGAGCT
    GGGGACTTGT GTCCAAACCA CCCTGTGGCA TCTGCTGACC TCCTGGGCTC ATCGCTTTTT
    GCCAACATCC CTGGTTACAA GCTTGGCTGC TACTTCTGCA ATGATGTGGT GGCCCCAGGA
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGTCGTCC AGGACTGGCC
    GTGATTGCAG GAGCCCTGGC CGTGGAATTG ATGGTATCTG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAT CGGATGAATG AGCCTCCAAC CTCTCTTGGG
    CTTGTGCCTC ACCAGATCCG GGGATTTCTT TCACGGTTTG ATAATGTCCT TCCCGTCAGC
    CTGGCATTTG ACAAATGTAC AGCTTGTTCT TCCAAAGTTC TTGATCAATA TGAACGAGAA
    GGATTTAACT TCCTAGCCAA GGTGTTTAAT TCTTCACATT CCTTCTTAGA AGACTTGACT
    GGTCTTACAT TGCTGCATCA AGAAACCCAA GCTGCTGAGG ATCATCTTTC TTTCATCCGG
    CCCAAGAATG GCACCTTCCC CGCCCATCAG GCCACCTGCA CTCAGGAGTC GGCTGCCTGT
    CCTGAAATAG ATCTCTACCA GCTCTCAAGC AGAACAATTC CTTGCATTCA TACTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016861031.1
    CDS35..2251
    Translation

    Target ORF information:

    RefSeq Version XM_017005542.1
    Organism Homo sapiens(human)
    Definition Homo sapiens autophagy related 7 (ATG7), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017005542.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCGGCAG CTACGGGGGA TCCTGGACTC TCTAAACTGC AGTTTGCCCC TTTTAGTAGT 
    GCCTTGGATG TTGGGTTTTG GCATGAGTTG ACCCAGAAGA AGCTGAACGA GTATCGGCTG
    GATGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAATG GTGACTCTGC TGGGCTGCCA
    GCTCGCTTAA CATTGGAGTT CAGTGCTTTT GACATGAGTG CTCCCACCCC AGCCCGTTGC
    TGCCCAGCTA TTGGAACACT GTATAACACC AACACACTCG AGTCTTTCAA GACTGCAGAT
    AAGAAGCTCC TTTTGGAACA AGCAGCAAAT GAGATATGGG AATCCATAAA ATCAGGCACT
    GCTCTTGAAA ACCCTGTACT CCTCAACAAG TTCCTCCTCT TGACATTTGC AGATCTAAAG
    AAGTACCACT TCTACTATTG GTTTTGCTAT CCTGCCCTCT GTCTTCCAGA GAGTTTACCT
    CTCATTCAGG GGCCAGTGGG TTTGGATCAA AGGTTTTCAC TAAAACAGAT TGAAGCACTA
    GAGTGTGCAT ATGATAATCT TTGTCAAACA GAAGGAGTCA CAGCTCTTCC TTACTTCTTA
    ATCAAGTATG ATGAGAACAT GGTGCTGGTT TCCTTGCTTA AACACTACAG TGATTTCTTC
    CAAGGTCAAA GGACGAAGAT AACAATTGGT GTATATGATC CCTGTAACTT AGCCCAGTAC
    CCTGGATGGC CTTTGAGGAA TTTTTTGGTC CTAGCAGCCC ACAGATGGAG TAGCAGTTTC
    CAGTCTGTTG AAGTTGTTTG CTTCCGTGAC CGTACCATGC AGGGGGCGAG AGACGTTGCC
    CACAGCATCA TCTTCGAAGT GAAGCTTCCA GAAATGGCAT TTAGCCCAGA TTGTCCTAAA
    GCAGTTGGAT GGGAAAAGAA CCAGAAAGGA GGCATGGGAC CAAGGATGGT GAACCTCAGT
    GAATGTATGG ACCCTAAAAG GTTAGCTGAG TCATCAGTGG ATCTAAATCT CAAACTGATG
    TGTTGGAGAT TGGTTCCTAC TTTAGACTTG GACAAGGTTG TGTCTGTCAA ATGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGCAATGTA GCTAGGACGT TGATGGGTTG GGGCGTGAGA
    CACATCACAT TTGTGGACAA TGCCAAGATC TCCTACTCCA ATCCTGTGAG GCAGCCTCTC
    TATGAGTTTG AAGATTGCCT AGGGGGTGGT AAGCCCAAGG CTCTGGCAGC AGCGGACCGG
    CTCCAGAAAA TATTCCCCGG TGTGAATGCC AGAGGATTCA ACATGAGCAT ACCTATGCCT
    GGGCATCCAG TGAACTTCTC CAGTGTCACT CTGGAGCAAG CCCGCAGAGA TGTGGAGCAA
    CTGGAGCAGC TCATCGAAAG CCATGATGTC GTCTTCCTAT TGATGGACAC CAGGGAGAGC
    CGGTGGCTTC CTGCCGTCAT TGCTGCAAGC AAGAGAAAGC TGGTCATCAA TGCTGCTTTG
    GGATTTGACA CATTTGTTGT CATGAGACAT GGTCTGAAGA AACCAAAGCA GCAAGGAGCT
    GGGGACTTGT GTCCAAACCA CCCTGTGGCA TCTGCTGACC TCCTGGGCTC ATCGCTTTTT
    GCCAACATCC CTGGTTACAA GCTTGGCTGC TACTTCTGCA ATGATGTGGT GGCCCCAGGA
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGTCGTCC AGGACTGGCC
    GTGATTGCAG GAGCCCTGGC CGTGGAATTG ATGGTATCTG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAT CGGATGAATG AGCCTCCAAC CTCTCTTGGG
    CTTGTGCCTC ACCAGATCCG GGGATTTCTT TCACGGTTTG ATAATGTCCT TCCCGTCAGC
    CTGGCATTTG ACAAATGTAC AGCTTGTTCT TCCAAAGTTC TTGATCAATA TGAACGAGAA
    GGATTTAACT TCCTAGCCAA GGTGTTTAAT TCTTCACATT CCTTCTTAGA AGACTTGACT
    GGTCTTACAT TGCTGCATCA AGAAACCCAA GCTGCTGAGG ATCATCTTTC TTTCATCCGG
    CCCAAGAATG GCACCTTCCC CGCCCATCAG GCCACCTGCA CTCAGGAGTC GGCTGCCTGT
    CCTGAAATAG ATCTCTACCA GCTCTCAAGC AGAACAATTC CTTGCATTCA TACTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu36913
    Clone ID Related Accession (Same CDS sequence) XM_006712932.4 , XM_011533278.2 , XM_006712932.3 , XM_011533277.3 , XM_017005542.1 , XM_006712931.3 , XM_006712931.4 , XM_006712933.3 , XM_011533277.2
    Accession Version XM_006712931.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2217bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product ubiquitin-like modifier-activating enzyme ATG7 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006712931.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCGGCAG CTACGGGGGA TCCTGGACTC TCTAAACTGC AGTTTGCCCC TTTTAGTAGT 
    GCCTTGGATG TTGGGTTTTG GCATGAGTTG ACCCAGAAGA AGCTGAACGA GTATCGGCTG
    GATGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAATG GTGACTCTGC TGGGCTGCCA
    GCTCGCTTAA CATTGGAGTT CAGTGCTTTT GACATGAGTG CTCCCACCCC AGCCCGTTGC
    TGCCCAGCTA TTGGAACACT GTATAACACC AACACACTCG AGTCTTTCAA GACTGCAGAT
    AAGAAGCTCC TTTTGGAACA AGCAGCAAAT GAGATATGGG AATCCATAAA ATCAGGCACT
    GCTCTTGAAA ACCCTGTACT CCTCAACAAG TTCCTCCTCT TGACATTTGC AGATCTAAAG
    AAGTACCACT TCTACTATTG GTTTTGCTAT CCTGCCCTCT GTCTTCCAGA GAGTTTACCT
    CTCATTCAGG GGCCAGTGGG TTTGGATCAA AGGTTTTCAC TAAAACAGAT TGAAGCACTA
    GAGTGTGCAT ATGATAATCT TTGTCAAACA GAAGGAGTCA CAGCTCTTCC TTACTTCTTA
    ATCAAGTATG ATGAGAACAT GGTGCTGGTT TCCTTGCTTA AACACTACAG TGATTTCTTC
    CAAGGTCAAA GGACGAAGAT AACAATTGGT GTATATGATC CCTGTAACTT AGCCCAGTAC
    CCTGGATGGC CTTTGAGGAA TTTTTTGGTC CTAGCAGCCC ACAGATGGAG TAGCAGTTTC
    CAGTCTGTTG AAGTTGTTTG CTTCCGTGAC CGTACCATGC AGGGGGCGAG AGACGTTGCC
    CACAGCATCA TCTTCGAAGT GAAGCTTCCA GAAATGGCAT TTAGCCCAGA TTGTCCTAAA
    GCAGTTGGAT GGGAAAAGAA CCAGAAAGGA GGCATGGGAC CAAGGATGGT GAACCTCAGT
    GAATGTATGG ACCCTAAAAG GTTAGCTGAG TCATCAGTGG ATCTAAATCT CAAACTGATG
    TGTTGGAGAT TGGTTCCTAC TTTAGACTTG GACAAGGTTG TGTCTGTCAA ATGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGCAATGTA GCTAGGACGT TGATGGGTTG GGGCGTGAGA
    CACATCACAT TTGTGGACAA TGCCAAGATC TCCTACTCCA ATCCTGTGAG GCAGCCTCTC
    TATGAGTTTG AAGATTGCCT AGGGGGTGGT AAGCCCAAGG CTCTGGCAGC AGCGGACCGG
    CTCCAGAAAA TATTCCCCGG TGTGAATGCC AGAGGATTCA ACATGAGCAT ACCTATGCCT
    GGGCATCCAG TGAACTTCTC CAGTGTCACT CTGGAGCAAG CCCGCAGAGA TGTGGAGCAA
    CTGGAGCAGC TCATCGAAAG CCATGATGTC GTCTTCCTAT TGATGGACAC CAGGGAGAGC
    CGGTGGCTTC CTGCCGTCAT TGCTGCAAGC AAGAGAAAGC TGGTCATCAA TGCTGCTTTG
    GGATTTGACA CATTTGTTGT CATGAGACAT GGTCTGAAGA AACCAAAGCA GCAAGGAGCT
    GGGGACTTGT GTCCAAACCA CCCTGTGGCA TCTGCTGACC TCCTGGGCTC ATCGCTTTTT
    GCCAACATCC CTGGTTACAA GCTTGGCTGC TACTTCTGCA ATGATGTGGT GGCCCCAGGA
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGTCGTCC AGGACTGGCC
    GTGATTGCAG GAGCCCTGGC CGTGGAATTG ATGGTATCTG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAT CGGATGAATG AGCCTCCAAC CTCTCTTGGG
    CTTGTGCCTC ACCAGATCCG GGGATTTCTT TCACGGTTTG ATAATGTCCT TCCCGTCAGC
    CTGGCATTTG ACAAATGTAC AGCTTGTTCT TCCAAAGTTC TTGATCAATA TGAACGAGAA
    GGATTTAACT TCCTAGCCAA GGTGTTTAAT TCTTCACATT CCTTCTTAGA AGACTTGACT
    GGTCTTACAT TGCTGCATCA AGAAACCCAA GCTGCTGAGG ATCATCTTTC TTTCATCCGG
    CCCAAGAATG GCACCTTCCC CGCCCATCAG GCCACCTGCA CTCAGGAGTC GGCTGCCTGT
    CCTGAAATAG ATCTCTACCA GCTCTCAAGC AGAACAATTC CTTGCATTCA TACTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006712994.1
    CDS61..2277
    Translation

    Target ORF information:

    RefSeq Version XM_006712931.3
    Organism Homo sapiens(human)
    Definition Homo sapiens autophagy related 7 (ATG7), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006712931.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCGGCAG CTACGGGGGA TCCTGGACTC TCTAAACTGC AGTTTGCCCC TTTTAGTAGT 
    GCCTTGGATG TTGGGTTTTG GCATGAGTTG ACCCAGAAGA AGCTGAACGA GTATCGGCTG
    GATGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAATG GTGACTCTGC TGGGCTGCCA
    GCTCGCTTAA CATTGGAGTT CAGTGCTTTT GACATGAGTG CTCCCACCCC AGCCCGTTGC
    TGCCCAGCTA TTGGAACACT GTATAACACC AACACACTCG AGTCTTTCAA GACTGCAGAT
    AAGAAGCTCC TTTTGGAACA AGCAGCAAAT GAGATATGGG AATCCATAAA ATCAGGCACT
    GCTCTTGAAA ACCCTGTACT CCTCAACAAG TTCCTCCTCT TGACATTTGC AGATCTAAAG
    AAGTACCACT TCTACTATTG GTTTTGCTAT CCTGCCCTCT GTCTTCCAGA GAGTTTACCT
    CTCATTCAGG GGCCAGTGGG TTTGGATCAA AGGTTTTCAC TAAAACAGAT TGAAGCACTA
    GAGTGTGCAT ATGATAATCT TTGTCAAACA GAAGGAGTCA CAGCTCTTCC TTACTTCTTA
    ATCAAGTATG ATGAGAACAT GGTGCTGGTT TCCTTGCTTA AACACTACAG TGATTTCTTC
    CAAGGTCAAA GGACGAAGAT AACAATTGGT GTATATGATC CCTGTAACTT AGCCCAGTAC
    CCTGGATGGC CTTTGAGGAA TTTTTTGGTC CTAGCAGCCC ACAGATGGAG TAGCAGTTTC
    CAGTCTGTTG AAGTTGTTTG CTTCCGTGAC CGTACCATGC AGGGGGCGAG AGACGTTGCC
    CACAGCATCA TCTTCGAAGT GAAGCTTCCA GAAATGGCAT TTAGCCCAGA TTGTCCTAAA
    GCAGTTGGAT GGGAAAAGAA CCAGAAAGGA GGCATGGGAC CAAGGATGGT GAACCTCAGT
    GAATGTATGG ACCCTAAAAG GTTAGCTGAG TCATCAGTGG ATCTAAATCT CAAACTGATG
    TGTTGGAGAT TGGTTCCTAC TTTAGACTTG GACAAGGTTG TGTCTGTCAA ATGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGCAATGTA GCTAGGACGT TGATGGGTTG GGGCGTGAGA
    CACATCACAT TTGTGGACAA TGCCAAGATC TCCTACTCCA ATCCTGTGAG GCAGCCTCTC
    TATGAGTTTG AAGATTGCCT AGGGGGTGGT AAGCCCAAGG CTCTGGCAGC AGCGGACCGG
    CTCCAGAAAA TATTCCCCGG TGTGAATGCC AGAGGATTCA ACATGAGCAT ACCTATGCCT
    GGGCATCCAG TGAACTTCTC CAGTGTCACT CTGGAGCAAG CCCGCAGAGA TGTGGAGCAA
    CTGGAGCAGC TCATCGAAAG CCATGATGTC GTCTTCCTAT TGATGGACAC CAGGGAGAGC
    CGGTGGCTTC CTGCCGTCAT TGCTGCAAGC AAGAGAAAGC TGGTCATCAA TGCTGCTTTG
    GGATTTGACA CATTTGTTGT CATGAGACAT GGTCTGAAGA AACCAAAGCA GCAAGGAGCT
    GGGGACTTGT GTCCAAACCA CCCTGTGGCA TCTGCTGACC TCCTGGGCTC ATCGCTTTTT
    GCCAACATCC CTGGTTACAA GCTTGGCTGC TACTTCTGCA ATGATGTGGT GGCCCCAGGA
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGTCGTCC AGGACTGGCC
    GTGATTGCAG GAGCCCTGGC CGTGGAATTG ATGGTATCTG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAT CGGATGAATG AGCCTCCAAC CTCTCTTGGG
    CTTGTGCCTC ACCAGATCCG GGGATTTCTT TCACGGTTTG ATAATGTCCT TCCCGTCAGC
    CTGGCATTTG ACAAATGTAC AGCTTGTTCT TCCAAAGTTC TTGATCAATA TGAACGAGAA
    GGATTTAACT TCCTAGCCAA GGTGTTTAAT TCTTCACATT CCTTCTTAGA AGACTTGACT
    GGTCTTACAT TGCTGCATCA AGAAACCCAA GCTGCTGAGG ATCATCTTTC TTTCATCCGG
    CCCAAGAATG GCACCTTCCC CGCCCATCAG GCCACCTGCA CTCAGGAGTC GGCTGCCTGT
    CCTGAAATAG ATCTCTACCA GCTCTCAAGC AGAACAATTC CTTGCATTCA TACTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu36913
    Clone ID Related Accession (Same CDS sequence) XM_006712932.4 , XM_011533278.2 , XM_006712932.3 , XM_011533277.3 , XM_017005542.1 , XM_006712931.3 , XM_006712931.4 , XM_006712933.3 , XM_011533277.2
    Accession Version XM_006712931.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2217bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product ubiquitin-like modifier-activating enzyme ATG7 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_006712931.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCGGCAG CTACGGGGGA TCCTGGACTC TCTAAACTGC AGTTTGCCCC TTTTAGTAGT 
    GCCTTGGATG TTGGGTTTTG GCATGAGTTG ACCCAGAAGA AGCTGAACGA GTATCGGCTG
    GATGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAATG GTGACTCTGC TGGGCTGCCA
    GCTCGCTTAA CATTGGAGTT CAGTGCTTTT GACATGAGTG CTCCCACCCC AGCCCGTTGC
    TGCCCAGCTA TTGGAACACT GTATAACACC AACACACTCG AGTCTTTCAA GACTGCAGAT
    AAGAAGCTCC TTTTGGAACA AGCAGCAAAT GAGATATGGG AATCCATAAA ATCAGGCACT
    GCTCTTGAAA ACCCTGTACT CCTCAACAAG TTCCTCCTCT TGACATTTGC AGATCTAAAG
    AAGTACCACT TCTACTATTG GTTTTGCTAT CCTGCCCTCT GTCTTCCAGA GAGTTTACCT
    CTCATTCAGG GGCCAGTGGG TTTGGATCAA AGGTTTTCAC TAAAACAGAT TGAAGCACTA
    GAGTGTGCAT ATGATAATCT TTGTCAAACA GAAGGAGTCA CAGCTCTTCC TTACTTCTTA
    ATCAAGTATG ATGAGAACAT GGTGCTGGTT TCCTTGCTTA AACACTACAG TGATTTCTTC
    CAAGGTCAAA GGACGAAGAT AACAATTGGT GTATATGATC CCTGTAACTT AGCCCAGTAC
    CCTGGATGGC CTTTGAGGAA TTTTTTGGTC CTAGCAGCCC ACAGATGGAG TAGCAGTTTC
    CAGTCTGTTG AAGTTGTTTG CTTCCGTGAC CGTACCATGC AGGGGGCGAG AGACGTTGCC
    CACAGCATCA TCTTCGAAGT GAAGCTTCCA GAAATGGCAT TTAGCCCAGA TTGTCCTAAA
    GCAGTTGGAT GGGAAAAGAA CCAGAAAGGA GGCATGGGAC CAAGGATGGT GAACCTCAGT
    GAATGTATGG ACCCTAAAAG GTTAGCTGAG TCATCAGTGG ATCTAAATCT CAAACTGATG
    TGTTGGAGAT TGGTTCCTAC TTTAGACTTG GACAAGGTTG TGTCTGTCAA ATGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGCAATGTA GCTAGGACGT TGATGGGTTG GGGCGTGAGA
    CACATCACAT TTGTGGACAA TGCCAAGATC TCCTACTCCA ATCCTGTGAG GCAGCCTCTC
    TATGAGTTTG AAGATTGCCT AGGGGGTGGT AAGCCCAAGG CTCTGGCAGC AGCGGACCGG
    CTCCAGAAAA TATTCCCCGG TGTGAATGCC AGAGGATTCA ACATGAGCAT ACCTATGCCT
    GGGCATCCAG TGAACTTCTC CAGTGTCACT CTGGAGCAAG CCCGCAGAGA TGTGGAGCAA
    CTGGAGCAGC TCATCGAAAG CCATGATGTC GTCTTCCTAT TGATGGACAC CAGGGAGAGC
    CGGTGGCTTC CTGCCGTCAT TGCTGCAAGC AAGAGAAAGC TGGTCATCAA TGCTGCTTTG
    GGATTTGACA CATTTGTTGT CATGAGACAT GGTCTGAAGA AACCAAAGCA GCAAGGAGCT
    GGGGACTTGT GTCCAAACCA CCCTGTGGCA TCTGCTGACC TCCTGGGCTC ATCGCTTTTT
    GCCAACATCC CTGGTTACAA GCTTGGCTGC TACTTCTGCA ATGATGTGGT GGCCCCAGGA
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGTCGTCC AGGACTGGCC
    GTGATTGCAG GAGCCCTGGC CGTGGAATTG ATGGTATCTG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAT CGGATGAATG AGCCTCCAAC CTCTCTTGGG
    CTTGTGCCTC ACCAGATCCG GGGATTTCTT TCACGGTTTG ATAATGTCCT TCCCGTCAGC
    CTGGCATTTG ACAAATGTAC AGCTTGTTCT TCCAAAGTTC TTGATCAATA TGAACGAGAA
    GGATTTAACT TCCTAGCCAA GGTGTTTAAT TCTTCACATT CCTTCTTAGA AGACTTGACT
    GGTCTTACAT TGCTGCATCA AGAAACCCAA GCTGCTGAGG ATCATCTTTC TTTCATCCGG
    CCCAAGAATG GCACCTTCCC CGCCCATCAG GCCACCTGCA CTCAGGAGTC GGCTGCCTGT
    CCTGAAATAG ATCTCTACCA GCTCTCAAGC AGAACAATTC CTTGCATTCA TACTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006712994.1
    CDS61..2277
    Translation

    Target ORF information:

    RefSeq Version XM_006712931.4
    Organism Homo sapiens(human)
    Definition Homo sapiens autophagy related 7 (ATG7), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006712931.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCGGCAG CTACGGGGGA TCCTGGACTC TCTAAACTGC AGTTTGCCCC TTTTAGTAGT 
    GCCTTGGATG TTGGGTTTTG GCATGAGTTG ACCCAGAAGA AGCTGAACGA GTATCGGCTG
    GATGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAATG GTGACTCTGC TGGGCTGCCA
    GCTCGCTTAA CATTGGAGTT CAGTGCTTTT GACATGAGTG CTCCCACCCC AGCCCGTTGC
    TGCCCAGCTA TTGGAACACT GTATAACACC AACACACTCG AGTCTTTCAA GACTGCAGAT
    AAGAAGCTCC TTTTGGAACA AGCAGCAAAT GAGATATGGG AATCCATAAA ATCAGGCACT
    GCTCTTGAAA ACCCTGTACT CCTCAACAAG TTCCTCCTCT TGACATTTGC AGATCTAAAG
    AAGTACCACT TCTACTATTG GTTTTGCTAT CCTGCCCTCT GTCTTCCAGA GAGTTTACCT
    CTCATTCAGG GGCCAGTGGG TTTGGATCAA AGGTTTTCAC TAAAACAGAT TGAAGCACTA
    GAGTGTGCAT ATGATAATCT TTGTCAAACA GAAGGAGTCA CAGCTCTTCC TTACTTCTTA
    ATCAAGTATG ATGAGAACAT GGTGCTGGTT TCCTTGCTTA AACACTACAG TGATTTCTTC
    CAAGGTCAAA GGACGAAGAT AACAATTGGT GTATATGATC CCTGTAACTT AGCCCAGTAC
    CCTGGATGGC CTTTGAGGAA TTTTTTGGTC CTAGCAGCCC ACAGATGGAG TAGCAGTTTC
    CAGTCTGTTG AAGTTGTTTG CTTCCGTGAC CGTACCATGC AGGGGGCGAG AGACGTTGCC
    CACAGCATCA TCTTCGAAGT GAAGCTTCCA GAAATGGCAT TTAGCCCAGA TTGTCCTAAA
    GCAGTTGGAT GGGAAAAGAA CCAGAAAGGA GGCATGGGAC CAAGGATGGT GAACCTCAGT
    GAATGTATGG ACCCTAAAAG GTTAGCTGAG TCATCAGTGG ATCTAAATCT CAAACTGATG
    TGTTGGAGAT TGGTTCCTAC TTTAGACTTG GACAAGGTTG TGTCTGTCAA ATGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGCAATGTA GCTAGGACGT TGATGGGTTG GGGCGTGAGA
    CACATCACAT TTGTGGACAA TGCCAAGATC TCCTACTCCA ATCCTGTGAG GCAGCCTCTC
    TATGAGTTTG AAGATTGCCT AGGGGGTGGT AAGCCCAAGG CTCTGGCAGC AGCGGACCGG
    CTCCAGAAAA TATTCCCCGG TGTGAATGCC AGAGGATTCA ACATGAGCAT ACCTATGCCT
    GGGCATCCAG TGAACTTCTC CAGTGTCACT CTGGAGCAAG CCCGCAGAGA TGTGGAGCAA
    CTGGAGCAGC TCATCGAAAG CCATGATGTC GTCTTCCTAT TGATGGACAC CAGGGAGAGC
    CGGTGGCTTC CTGCCGTCAT TGCTGCAAGC AAGAGAAAGC TGGTCATCAA TGCTGCTTTG
    GGATTTGACA CATTTGTTGT CATGAGACAT GGTCTGAAGA AACCAAAGCA GCAAGGAGCT
    GGGGACTTGT GTCCAAACCA CCCTGTGGCA TCTGCTGACC TCCTGGGCTC ATCGCTTTTT
    GCCAACATCC CTGGTTACAA GCTTGGCTGC TACTTCTGCA ATGATGTGGT GGCCCCAGGA
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGTCGTCC AGGACTGGCC
    GTGATTGCAG GAGCCCTGGC CGTGGAATTG ATGGTATCTG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAT CGGATGAATG AGCCTCCAAC CTCTCTTGGG
    CTTGTGCCTC ACCAGATCCG GGGATTTCTT TCACGGTTTG ATAATGTCCT TCCCGTCAGC
    CTGGCATTTG ACAAATGTAC AGCTTGTTCT TCCAAAGTTC TTGATCAATA TGAACGAGAA
    GGATTTAACT TCCTAGCCAA GGTGTTTAAT TCTTCACATT CCTTCTTAGA AGACTTGACT
    GGTCTTACAT TGCTGCATCA AGAAACCCAA GCTGCTGAGG ATCATCTTTC TTTCATCCGG
    CCCAAGAATG GCACCTTCCC CGCCCATCAG GCCACCTGCA CTCAGGAGTC GGCTGCCTGT
    CCTGAAATAG ATCTCTACCA GCTCTCAAGC AGAACAATTC CTTGCATTCA TACTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu36913
    Clone ID Related Accession (Same CDS sequence) XM_006712932.4 , XM_011533278.2 , XM_006712932.3 , XM_011533277.3 , XM_017005542.1 , XM_006712931.3 , XM_006712931.4 , XM_006712933.3 , XM_011533277.2
    Accession Version XM_006712933.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2217bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product ubiquitin-like modifier-activating enzyme ATG7 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006712933.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCGGCAG CTACGGGGGA TCCTGGACTC TCTAAACTGC AGTTTGCCCC TTTTAGTAGT 
    GCCTTGGATG TTGGGTTTTG GCATGAGTTG ACCCAGAAGA AGCTGAACGA GTATCGGCTG
    GATGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAATG GTGACTCTGC TGGGCTGCCA
    GCTCGCTTAA CATTGGAGTT CAGTGCTTTT GACATGAGTG CTCCCACCCC AGCCCGTTGC
    TGCCCAGCTA TTGGAACACT GTATAACACC AACACACTCG AGTCTTTCAA GACTGCAGAT
    AAGAAGCTCC TTTTGGAACA AGCAGCAAAT GAGATATGGG AATCCATAAA ATCAGGCACT
    GCTCTTGAAA ACCCTGTACT CCTCAACAAG TTCCTCCTCT TGACATTTGC AGATCTAAAG
    AAGTACCACT TCTACTATTG GTTTTGCTAT CCTGCCCTCT GTCTTCCAGA GAGTTTACCT
    CTCATTCAGG GGCCAGTGGG TTTGGATCAA AGGTTTTCAC TAAAACAGAT TGAAGCACTA
    GAGTGTGCAT ATGATAATCT TTGTCAAACA GAAGGAGTCA CAGCTCTTCC TTACTTCTTA
    ATCAAGTATG ATGAGAACAT GGTGCTGGTT TCCTTGCTTA AACACTACAG TGATTTCTTC
    CAAGGTCAAA GGACGAAGAT AACAATTGGT GTATATGATC CCTGTAACTT AGCCCAGTAC
    CCTGGATGGC CTTTGAGGAA TTTTTTGGTC CTAGCAGCCC ACAGATGGAG TAGCAGTTTC
    CAGTCTGTTG AAGTTGTTTG CTTCCGTGAC CGTACCATGC AGGGGGCGAG AGACGTTGCC
    CACAGCATCA TCTTCGAAGT GAAGCTTCCA GAAATGGCAT TTAGCCCAGA TTGTCCTAAA
    GCAGTTGGAT GGGAAAAGAA CCAGAAAGGA GGCATGGGAC CAAGGATGGT GAACCTCAGT
    GAATGTATGG ACCCTAAAAG GTTAGCTGAG TCATCAGTGG ATCTAAATCT CAAACTGATG
    TGTTGGAGAT TGGTTCCTAC TTTAGACTTG GACAAGGTTG TGTCTGTCAA ATGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGCAATGTA GCTAGGACGT TGATGGGTTG GGGCGTGAGA
    CACATCACAT TTGTGGACAA TGCCAAGATC TCCTACTCCA ATCCTGTGAG GCAGCCTCTC
    TATGAGTTTG AAGATTGCCT AGGGGGTGGT AAGCCCAAGG CTCTGGCAGC AGCGGACCGG
    CTCCAGAAAA TATTCCCCGG TGTGAATGCC AGAGGATTCA ACATGAGCAT ACCTATGCCT
    GGGCATCCAG TGAACTTCTC CAGTGTCACT CTGGAGCAAG CCCGCAGAGA TGTGGAGCAA
    CTGGAGCAGC TCATCGAAAG CCATGATGTC GTCTTCCTAT TGATGGACAC CAGGGAGAGC
    CGGTGGCTTC CTGCCGTCAT TGCTGCAAGC AAGAGAAAGC TGGTCATCAA TGCTGCTTTG
    GGATTTGACA CATTTGTTGT CATGAGACAT GGTCTGAAGA AACCAAAGCA GCAAGGAGCT
    GGGGACTTGT GTCCAAACCA CCCTGTGGCA TCTGCTGACC TCCTGGGCTC ATCGCTTTTT
    GCCAACATCC CTGGTTACAA GCTTGGCTGC TACTTCTGCA ATGATGTGGT GGCCCCAGGA
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGTCGTCC AGGACTGGCC
    GTGATTGCAG GAGCCCTGGC CGTGGAATTG ATGGTATCTG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAT CGGATGAATG AGCCTCCAAC CTCTCTTGGG
    CTTGTGCCTC ACCAGATCCG GGGATTTCTT TCACGGTTTG ATAATGTCCT TCCCGTCAGC
    CTGGCATTTG ACAAATGTAC AGCTTGTTCT TCCAAAGTTC TTGATCAATA TGAACGAGAA
    GGATTTAACT TCCTAGCCAA GGTGTTTAAT TCTTCACATT CCTTCTTAGA AGACTTGACT
    GGTCTTACAT TGCTGCATCA AGAAACCCAA GCTGCTGAGG ATCATCTTTC TTTCATCCGG
    CCCAAGAATG GCACCTTCCC CGCCCATCAG GCCACCTGCA CTCAGGAGTC GGCTGCCTGT
    CCTGAAATAG ATCTCTACCA GCTCTCAAGC AGAACAATTC CTTGCATTCA TACTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006712996.1
    CDS298..2514
    Translation

    Target ORF information:

    RefSeq Version XM_006712933.3
    Organism Homo sapiens(human)
    Definition Homo sapiens autophagy related 7 (ATG7), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006712933.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCGGCAG CTACGGGGGA TCCTGGACTC TCTAAACTGC AGTTTGCCCC TTTTAGTAGT 
    GCCTTGGATG TTGGGTTTTG GCATGAGTTG ACCCAGAAGA AGCTGAACGA GTATCGGCTG
    GATGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAATG GTGACTCTGC TGGGCTGCCA
    GCTCGCTTAA CATTGGAGTT CAGTGCTTTT GACATGAGTG CTCCCACCCC AGCCCGTTGC
    TGCCCAGCTA TTGGAACACT GTATAACACC AACACACTCG AGTCTTTCAA GACTGCAGAT
    AAGAAGCTCC TTTTGGAACA AGCAGCAAAT GAGATATGGG AATCCATAAA ATCAGGCACT
    GCTCTTGAAA ACCCTGTACT CCTCAACAAG TTCCTCCTCT TGACATTTGC AGATCTAAAG
    AAGTACCACT TCTACTATTG GTTTTGCTAT CCTGCCCTCT GTCTTCCAGA GAGTTTACCT
    CTCATTCAGG GGCCAGTGGG TTTGGATCAA AGGTTTTCAC TAAAACAGAT TGAAGCACTA
    GAGTGTGCAT ATGATAATCT TTGTCAAACA GAAGGAGTCA CAGCTCTTCC TTACTTCTTA
    ATCAAGTATG ATGAGAACAT GGTGCTGGTT TCCTTGCTTA AACACTACAG TGATTTCTTC
    CAAGGTCAAA GGACGAAGAT AACAATTGGT GTATATGATC CCTGTAACTT AGCCCAGTAC
    CCTGGATGGC CTTTGAGGAA TTTTTTGGTC CTAGCAGCCC ACAGATGGAG TAGCAGTTTC
    CAGTCTGTTG AAGTTGTTTG CTTCCGTGAC CGTACCATGC AGGGGGCGAG AGACGTTGCC
    CACAGCATCA TCTTCGAAGT GAAGCTTCCA GAAATGGCAT TTAGCCCAGA TTGTCCTAAA
    GCAGTTGGAT GGGAAAAGAA CCAGAAAGGA GGCATGGGAC CAAGGATGGT GAACCTCAGT
    GAATGTATGG ACCCTAAAAG GTTAGCTGAG TCATCAGTGG ATCTAAATCT CAAACTGATG
    TGTTGGAGAT TGGTTCCTAC TTTAGACTTG GACAAGGTTG TGTCTGTCAA ATGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGCAATGTA GCTAGGACGT TGATGGGTTG GGGCGTGAGA
    CACATCACAT TTGTGGACAA TGCCAAGATC TCCTACTCCA ATCCTGTGAG GCAGCCTCTC
    TATGAGTTTG AAGATTGCCT AGGGGGTGGT AAGCCCAAGG CTCTGGCAGC AGCGGACCGG
    CTCCAGAAAA TATTCCCCGG TGTGAATGCC AGAGGATTCA ACATGAGCAT ACCTATGCCT
    GGGCATCCAG TGAACTTCTC CAGTGTCACT CTGGAGCAAG CCCGCAGAGA TGTGGAGCAA
    CTGGAGCAGC TCATCGAAAG CCATGATGTC GTCTTCCTAT TGATGGACAC CAGGGAGAGC
    CGGTGGCTTC CTGCCGTCAT TGCTGCAAGC AAGAGAAAGC TGGTCATCAA TGCTGCTTTG
    GGATTTGACA CATTTGTTGT CATGAGACAT GGTCTGAAGA AACCAAAGCA GCAAGGAGCT
    GGGGACTTGT GTCCAAACCA CCCTGTGGCA TCTGCTGACC TCCTGGGCTC ATCGCTTTTT
    GCCAACATCC CTGGTTACAA GCTTGGCTGC TACTTCTGCA ATGATGTGGT GGCCCCAGGA
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGTCGTCC AGGACTGGCC
    GTGATTGCAG GAGCCCTGGC CGTGGAATTG ATGGTATCTG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAT CGGATGAATG AGCCTCCAAC CTCTCTTGGG
    CTTGTGCCTC ACCAGATCCG GGGATTTCTT TCACGGTTTG ATAATGTCCT TCCCGTCAGC
    CTGGCATTTG ACAAATGTAC AGCTTGTTCT TCCAAAGTTC TTGATCAATA TGAACGAGAA
    GGATTTAACT TCCTAGCCAA GGTGTTTAAT TCTTCACATT CCTTCTTAGA AGACTTGACT
    GGTCTTACAT TGCTGCATCA AGAAACCCAA GCTGCTGAGG ATCATCTTTC TTTCATCCGG
    CCCAAGAATG GCACCTTCCC CGCCCATCAG GCCACCTGCA CTCAGGAGTC GGCTGCCTGT
    CCTGAAATAG ATCTCTACCA GCTCTCAAGC AGAACAATTC CTTGCATTCA TACTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu36913
    Clone ID Related Accession (Same CDS sequence) XM_006712932.4 , XM_011533278.2 , XM_006712932.3 , XM_011533277.3 , XM_017005542.1 , XM_006712931.3 , XM_006712931.4 , XM_006712933.3 , XM_011533277.2
    Accession Version XM_011533277.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2217bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product ubiquitin-like modifier-activating enzyme ATG7 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011533277.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCGGCAG CTACGGGGGA TCCTGGACTC TCTAAACTGC AGTTTGCCCC TTTTAGTAGT 
    GCCTTGGATG TTGGGTTTTG GCATGAGTTG ACCCAGAAGA AGCTGAACGA GTATCGGCTG
    GATGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAATG GTGACTCTGC TGGGCTGCCA
    GCTCGCTTAA CATTGGAGTT CAGTGCTTTT GACATGAGTG CTCCCACCCC AGCCCGTTGC
    TGCCCAGCTA TTGGAACACT GTATAACACC AACACACTCG AGTCTTTCAA GACTGCAGAT
    AAGAAGCTCC TTTTGGAACA AGCAGCAAAT GAGATATGGG AATCCATAAA ATCAGGCACT
    GCTCTTGAAA ACCCTGTACT CCTCAACAAG TTCCTCCTCT TGACATTTGC AGATCTAAAG
    AAGTACCACT TCTACTATTG GTTTTGCTAT CCTGCCCTCT GTCTTCCAGA GAGTTTACCT
    CTCATTCAGG GGCCAGTGGG TTTGGATCAA AGGTTTTCAC TAAAACAGAT TGAAGCACTA
    GAGTGTGCAT ATGATAATCT TTGTCAAACA GAAGGAGTCA CAGCTCTTCC TTACTTCTTA
    ATCAAGTATG ATGAGAACAT GGTGCTGGTT TCCTTGCTTA AACACTACAG TGATTTCTTC
    CAAGGTCAAA GGACGAAGAT AACAATTGGT GTATATGATC CCTGTAACTT AGCCCAGTAC
    CCTGGATGGC CTTTGAGGAA TTTTTTGGTC CTAGCAGCCC ACAGATGGAG TAGCAGTTTC
    CAGTCTGTTG AAGTTGTTTG CTTCCGTGAC CGTACCATGC AGGGGGCGAG AGACGTTGCC
    CACAGCATCA TCTTCGAAGT GAAGCTTCCA GAAATGGCAT TTAGCCCAGA TTGTCCTAAA
    GCAGTTGGAT GGGAAAAGAA CCAGAAAGGA GGCATGGGAC CAAGGATGGT GAACCTCAGT
    GAATGTATGG ACCCTAAAAG GTTAGCTGAG TCATCAGTGG ATCTAAATCT CAAACTGATG
    TGTTGGAGAT TGGTTCCTAC TTTAGACTTG GACAAGGTTG TGTCTGTCAA ATGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGCAATGTA GCTAGGACGT TGATGGGTTG GGGCGTGAGA
    CACATCACAT TTGTGGACAA TGCCAAGATC TCCTACTCCA ATCCTGTGAG GCAGCCTCTC
    TATGAGTTTG AAGATTGCCT AGGGGGTGGT AAGCCCAAGG CTCTGGCAGC AGCGGACCGG
    CTCCAGAAAA TATTCCCCGG TGTGAATGCC AGAGGATTCA ACATGAGCAT ACCTATGCCT
    GGGCATCCAG TGAACTTCTC CAGTGTCACT CTGGAGCAAG CCCGCAGAGA TGTGGAGCAA
    CTGGAGCAGC TCATCGAAAG CCATGATGTC GTCTTCCTAT TGATGGACAC CAGGGAGAGC
    CGGTGGCTTC CTGCCGTCAT TGCTGCAAGC AAGAGAAAGC TGGTCATCAA TGCTGCTTTG
    GGATTTGACA CATTTGTTGT CATGAGACAT GGTCTGAAGA AACCAAAGCA GCAAGGAGCT
    GGGGACTTGT GTCCAAACCA CCCTGTGGCA TCTGCTGACC TCCTGGGCTC ATCGCTTTTT
    GCCAACATCC CTGGTTACAA GCTTGGCTGC TACTTCTGCA ATGATGTGGT GGCCCCAGGA
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGTCGTCC AGGACTGGCC
    GTGATTGCAG GAGCCCTGGC CGTGGAATTG ATGGTATCTG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAT CGGATGAATG AGCCTCCAAC CTCTCTTGGG
    CTTGTGCCTC ACCAGATCCG GGGATTTCTT TCACGGTTTG ATAATGTCCT TCCCGTCAGC
    CTGGCATTTG ACAAATGTAC AGCTTGTTCT TCCAAAGTTC TTGATCAATA TGAACGAGAA
    GGATTTAACT TCCTAGCCAA GGTGTTTAAT TCTTCACATT CCTTCTTAGA AGACTTGACT
    GGTCTTACAT TGCTGCATCA AGAAACCCAA GCTGCTGAGG ATCATCTTTC TTTCATCCGG
    CCCAAGAATG GCACCTTCCC CGCCCATCAG GCCACCTGCA CTCAGGAGTC GGCTGCCTGT
    CCTGAAATAG ATCTCTACCA GCTCTCAAGC AGAACAATTC CTTGCATTCA TACTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011531579.1
    CDS449..2665
    Translation

    Target ORF information:

    RefSeq Version XM_011533277.2
    Organism Homo sapiens(human)
    Definition Homo sapiens autophagy related 7 (ATG7), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011533277.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCGGCAG CTACGGGGGA TCCTGGACTC TCTAAACTGC AGTTTGCCCC TTTTAGTAGT 
    GCCTTGGATG TTGGGTTTTG GCATGAGTTG ACCCAGAAGA AGCTGAACGA GTATCGGCTG
    GATGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAATG GTGACTCTGC TGGGCTGCCA
    GCTCGCTTAA CATTGGAGTT CAGTGCTTTT GACATGAGTG CTCCCACCCC AGCCCGTTGC
    TGCCCAGCTA TTGGAACACT GTATAACACC AACACACTCG AGTCTTTCAA GACTGCAGAT
    AAGAAGCTCC TTTTGGAACA AGCAGCAAAT GAGATATGGG AATCCATAAA ATCAGGCACT
    GCTCTTGAAA ACCCTGTACT CCTCAACAAG TTCCTCCTCT TGACATTTGC AGATCTAAAG
    AAGTACCACT TCTACTATTG GTTTTGCTAT CCTGCCCTCT GTCTTCCAGA GAGTTTACCT
    CTCATTCAGG GGCCAGTGGG TTTGGATCAA AGGTTTTCAC TAAAACAGAT TGAAGCACTA
    GAGTGTGCAT ATGATAATCT TTGTCAAACA GAAGGAGTCA CAGCTCTTCC TTACTTCTTA
    ATCAAGTATG ATGAGAACAT GGTGCTGGTT TCCTTGCTTA AACACTACAG TGATTTCTTC
    CAAGGTCAAA GGACGAAGAT AACAATTGGT GTATATGATC CCTGTAACTT AGCCCAGTAC
    CCTGGATGGC CTTTGAGGAA TTTTTTGGTC CTAGCAGCCC ACAGATGGAG TAGCAGTTTC
    CAGTCTGTTG AAGTTGTTTG CTTCCGTGAC CGTACCATGC AGGGGGCGAG AGACGTTGCC
    CACAGCATCA TCTTCGAAGT GAAGCTTCCA GAAATGGCAT TTAGCCCAGA TTGTCCTAAA
    GCAGTTGGAT GGGAAAAGAA CCAGAAAGGA GGCATGGGAC CAAGGATGGT GAACCTCAGT
    GAATGTATGG ACCCTAAAAG GTTAGCTGAG TCATCAGTGG ATCTAAATCT CAAACTGATG
    TGTTGGAGAT TGGTTCCTAC TTTAGACTTG GACAAGGTTG TGTCTGTCAA ATGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGCAATGTA GCTAGGACGT TGATGGGTTG GGGCGTGAGA
    CACATCACAT TTGTGGACAA TGCCAAGATC TCCTACTCCA ATCCTGTGAG GCAGCCTCTC
    TATGAGTTTG AAGATTGCCT AGGGGGTGGT AAGCCCAAGG CTCTGGCAGC AGCGGACCGG
    CTCCAGAAAA TATTCCCCGG TGTGAATGCC AGAGGATTCA ACATGAGCAT ACCTATGCCT
    GGGCATCCAG TGAACTTCTC CAGTGTCACT CTGGAGCAAG CCCGCAGAGA TGTGGAGCAA
    CTGGAGCAGC TCATCGAAAG CCATGATGTC GTCTTCCTAT TGATGGACAC CAGGGAGAGC
    CGGTGGCTTC CTGCCGTCAT TGCTGCAAGC AAGAGAAAGC TGGTCATCAA TGCTGCTTTG
    GGATTTGACA CATTTGTTGT CATGAGACAT GGTCTGAAGA AACCAAAGCA GCAAGGAGCT
    GGGGACTTGT GTCCAAACCA CCCTGTGGCA TCTGCTGACC TCCTGGGCTC ATCGCTTTTT
    GCCAACATCC CTGGTTACAA GCTTGGCTGC TACTTCTGCA ATGATGTGGT GGCCCCAGGA
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGTCGTCC AGGACTGGCC
    GTGATTGCAG GAGCCCTGGC CGTGGAATTG ATGGTATCTG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAT CGGATGAATG AGCCTCCAAC CTCTCTTGGG
    CTTGTGCCTC ACCAGATCCG GGGATTTCTT TCACGGTTTG ATAATGTCCT TCCCGTCAGC
    CTGGCATTTG ACAAATGTAC AGCTTGTTCT TCCAAAGTTC TTGATCAATA TGAACGAGAA
    GGATTTAACT TCCTAGCCAA GGTGTTTAAT TCTTCACATT CCTTCTTAGA AGACTTGACT
    GGTCTTACAT TGCTGCATCA AGAAACCCAA GCTGCTGAGG ATCATCTTTC TTTCATCCGG
    CCCAAGAATG GCACCTTCCC CGCCCATCAG GCCACCTGCA CTCAGGAGTC GGCTGCCTGT
    CCTGAAATAG ATCTCTACCA GCTCTCAAGC AGAACAATTC CTTGCATTCA TACTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu36915
    Clone ID Related Accession (Same CDS sequence) XM_011533283.2 , XM_017005548.1
    Accession Version XM_011533283.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2100bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product ubiquitin-like modifier-activating enzyme ATG7 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011533283.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGCGGCAG CTACGGGGGA TCCTGGACTC TCTAAACTGC AGTTTGCCCC TTTTAGTAGT 
    GCCTTGGATG TTGGGTTTTG GCATGAGTTG ACCCAGAAGA AGCTGAACGA GTATCGGCTG
    GATGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAATG GTGACTCTGC TGGGCTGCCA
    GCTCGCTTAA CATTGGAGTT CAGTGCTTTT GACATGAGTG CTCCCACCCC AGCCCGTTGC
    TGCCCAGCTA TTGGAACACT GTATAACACC AACACACTCG AGTCTTTCAA GACTGCAGAT
    AAGAAGCTCC TTTTGGAACA AGCAGCAAAT GAGATATGGG AATCCATAAA ATCAGGCACT
    GCTCTTGAAA ACCCTGTACT CCTCAACAAG TTCCTCCTCT TGACATTTGC AGATCTAAAG
    AAGTACCACT TCTACTATTG GTTTTGCTAT CCTGCCCTCT GTCTTCCAGA GAGTTTACCT
    CTCATTCAGG GGCCAGTGGG TTTGGATCAA AGGTTTTCAC TAAAACAGAT TGAAGCACTA
    GAGTGTGCAT ATGATAATCT TTGTCAAACA GAAGGAGTCA CAGCTCTTCC TTACTTCTTA
    ATCAAGTATG ATGAGAACAT GGTGCTGGTT TCCTTGCTTA AACACTACAG TGATTTCTTC
    CAAGGTCAAA GGACGAAGAT AACAATTGGT GTATATGATC CCTGTAACTT AGCCCAGTAC
    CCTGGATGGC CTTTGAGGAA TTTTTTGGTC CTAGCAGCCC ACAGATGGAG TAGCAGTTTC
    CAGTCTGTTG AAGTTGTTTG CTTCCGTGAC CGTACCATGC AGGGGGCGAG AGACGTTGCC
    CACAGCATCA TCTTCGAAGT GAAGCTTCCA GAAATGGCAT TTAGCCCAGA TTGTCCTAAA
    GCAGTTGGAT GGGAAAAGAA CCAGAAAGGA GGCATGGGAC CAAGGATGGT GAACCTCAGT
    GAATGTATGG ACCCTAAAAG GTTAGCTGAG TCATCAGTGG ATCTAAATCT CAAACTGATG
    TGTTGGAGAT TGGTTCCTAC TTTAGACTTG GACAAGGTTG TGTCTGTCAA ATGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGCAATGTA GCTAGGACGT TGATGGGTTG GGGCGTGAGA
    CACATCACAT TTGTGGACAA TGCCAAGATC TCCTACTCCA ATCCTGTGAG GCAGCCTCTC
    TATGAGTTTG AAGATTGCCT AGGGGGTGGT AAGCCCAAGG CTCTGGCAGC AGCGGACCGG
    CTCCAGAAAA TATTCCCCGG TGTGAATGCC AGAGGATTCA ACATGAGCAT ACCTATGCCT
    GGGCATCCAG TGAACTTCTC CAGTGTCACT CTGGAGCAAG CCCGCAGAGA TGTGGAGCAA
    CTGGAGCAGC TCATCGAAAG CCATGATGTC GTCTTCCTAT TGATGGACAC CAGGGAGAGC
    CGGTGGCTTC CTGCCGTCAT TGCTGCAAGC AAGAGAAAGC TGGTCATCAA TGCTGCTTTG
    GGATTTGACA CATTTGTTGT CATGAGACAT GGTCTGAAGA AACCAAAGCA GCAAGGAGCT
    GGGGACTTGT GTCCAAACCA CCCTGTGGCA TCTGCTGACC TCCTGGGCTC ATCGCTTTTT
    GCCAACATCC CTGGTTACAA GCTTGGCTGC TACTTCTGCA ATGATGTGGT GGCCCCAGGA
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGTCGTCC AGGACTGGCC
    GTGATTGCAG GAGCCCTGGC CGTGGAATTG ATGGTATCTG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAT CGGATGAATG AGCCTCCAAC CTCTCTTGGG
    CTTGTGCCTC ACCAGATCCG GGGATTTCTT TCACGGTTTG ATAATGTCCT TCCCGTCAGC
    CTGGCATTTG ACAAATGTAC AGCTTGTTCT TCCAAAGTTC TTGATCAATA TGAACGAGAA
    GGATTTAACT TCCTAGCCAA GGTGTTTAAT TCTTCACATT CCTTCTTAGA AGACTTGACT
    GGTCTTACAT TGCTGCATCA AGAAACCCAA GCTGCTGAGG CAGAATATGA AATTCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011531585.1
    CDS449..2548
    Translation

    Target ORF information:

    RefSeq Version XM_011533283.2
    Organism Homo sapiens(human)
    Definition Homo sapiens autophagy related 7 (ATG7), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011533283.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGCGGCAG CTACGGGGGA TCCTGGACTC TCTAAACTGC AGTTTGCCCC TTTTAGTAGT 
    GCCTTGGATG TTGGGTTTTG GCATGAGTTG ACCCAGAAGA AGCTGAACGA GTATCGGCTG
    GATGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAATG GTGACTCTGC TGGGCTGCCA
    GCTCGCTTAA CATTGGAGTT CAGTGCTTTT GACATGAGTG CTCCCACCCC AGCCCGTTGC
    TGCCCAGCTA TTGGAACACT GTATAACACC AACACACTCG AGTCTTTCAA GACTGCAGAT
    AAGAAGCTCC TTTTGGAACA AGCAGCAAAT GAGATATGGG AATCCATAAA ATCAGGCACT
    GCTCTTGAAA ACCCTGTACT CCTCAACAAG TTCCTCCTCT TGACATTTGC AGATCTAAAG
    AAGTACCACT TCTACTATTG GTTTTGCTAT CCTGCCCTCT GTCTTCCAGA GAGTTTACCT
    CTCATTCAGG GGCCAGTGGG TTTGGATCAA AGGTTTTCAC TAAAACAGAT TGAAGCACTA
    GAGTGTGCAT ATGATAATCT TTGTCAAACA GAAGGAGTCA CAGCTCTTCC TTACTTCTTA
    ATCAAGTATG ATGAGAACAT GGTGCTGGTT TCCTTGCTTA AACACTACAG TGATTTCTTC
    CAAGGTCAAA GGACGAAGAT AACAATTGGT GTATATGATC CCTGTAACTT AGCCCAGTAC
    CCTGGATGGC CTTTGAGGAA TTTTTTGGTC CTAGCAGCCC ACAGATGGAG TAGCAGTTTC
    CAGTCTGTTG AAGTTGTTTG CTTCCGTGAC CGTACCATGC AGGGGGCGAG AGACGTTGCC
    CACAGCATCA TCTTCGAAGT GAAGCTTCCA GAAATGGCAT TTAGCCCAGA TTGTCCTAAA
    GCAGTTGGAT GGGAAAAGAA CCAGAAAGGA GGCATGGGAC CAAGGATGGT GAACCTCAGT
    GAATGTATGG ACCCTAAAAG GTTAGCTGAG TCATCAGTGG ATCTAAATCT CAAACTGATG
    TGTTGGAGAT TGGTTCCTAC TTTAGACTTG GACAAGGTTG TGTCTGTCAA ATGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGCAATGTA GCTAGGACGT TGATGGGTTG GGGCGTGAGA
    CACATCACAT TTGTGGACAA TGCCAAGATC TCCTACTCCA ATCCTGTGAG GCAGCCTCTC
    TATGAGTTTG AAGATTGCCT AGGGGGTGGT AAGCCCAAGG CTCTGGCAGC AGCGGACCGG
    CTCCAGAAAA TATTCCCCGG TGTGAATGCC AGAGGATTCA ACATGAGCAT ACCTATGCCT
    GGGCATCCAG TGAACTTCTC CAGTGTCACT CTGGAGCAAG CCCGCAGAGA TGTGGAGCAA
    CTGGAGCAGC TCATCGAAAG CCATGATGTC GTCTTCCTAT TGATGGACAC CAGGGAGAGC
    CGGTGGCTTC CTGCCGTCAT TGCTGCAAGC AAGAGAAAGC TGGTCATCAA TGCTGCTTTG
    GGATTTGACA CATTTGTTGT CATGAGACAT GGTCTGAAGA AACCAAAGCA GCAAGGAGCT
    GGGGACTTGT GTCCAAACCA CCCTGTGGCA TCTGCTGACC TCCTGGGCTC ATCGCTTTTT
    GCCAACATCC CTGGTTACAA GCTTGGCTGC TACTTCTGCA ATGATGTGGT GGCCCCAGGA
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGTCGTCC AGGACTGGCC
    GTGATTGCAG GAGCCCTGGC CGTGGAATTG ATGGTATCTG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAT CGGATGAATG AGCCTCCAAC CTCTCTTGGG
    CTTGTGCCTC ACCAGATCCG GGGATTTCTT TCACGGTTTG ATAATGTCCT TCCCGTCAGC
    CTGGCATTTG ACAAATGTAC AGCTTGTTCT TCCAAAGTTC TTGATCAATA TGAACGAGAA
    GGATTTAACT TCCTAGCCAA GGTGTTTAAT TCTTCACATT CCTTCTTAGA AGACTTGACT
    GGTCTTACAT TGCTGCATCA AGAAACCCAA GCTGCTGAGG CAGAATATGA AATTCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu36915
    Clone ID Related Accession (Same CDS sequence) XM_011533283.2 , XM_017005548.1
    Accession Version XM_017005548.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2100bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product ubiquitin-like modifier-activating enzyme ATG7 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGCGGCAG CTACGGGGGA TCCTGGACTC TCTAAACTGC AGTTTGCCCC TTTTAGTAGT 
    GCCTTGGATG TTGGGTTTTG GCATGAGTTG ACCCAGAAGA AGCTGAACGA GTATCGGCTG
    GATGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAATG GTGACTCTGC TGGGCTGCCA
    GCTCGCTTAA CATTGGAGTT CAGTGCTTTT GACATGAGTG CTCCCACCCC AGCCCGTTGC
    TGCCCAGCTA TTGGAACACT GTATAACACC AACACACTCG AGTCTTTCAA GACTGCAGAT
    AAGAAGCTCC TTTTGGAACA AGCAGCAAAT GAGATATGGG AATCCATAAA ATCAGGCACT
    GCTCTTGAAA ACCCTGTACT CCTCAACAAG TTCCTCCTCT TGACATTTGC AGATCTAAAG
    AAGTACCACT TCTACTATTG GTTTTGCTAT CCTGCCCTCT GTCTTCCAGA GAGTTTACCT
    CTCATTCAGG GGCCAGTGGG TTTGGATCAA AGGTTTTCAC TAAAACAGAT TGAAGCACTA
    GAGTGTGCAT ATGATAATCT TTGTCAAACA GAAGGAGTCA CAGCTCTTCC TTACTTCTTA
    ATCAAGTATG ATGAGAACAT GGTGCTGGTT TCCTTGCTTA AACACTACAG TGATTTCTTC
    CAAGGTCAAA GGACGAAGAT AACAATTGGT GTATATGATC CCTGTAACTT AGCCCAGTAC
    CCTGGATGGC CTTTGAGGAA TTTTTTGGTC CTAGCAGCCC ACAGATGGAG TAGCAGTTTC
    CAGTCTGTTG AAGTTGTTTG CTTCCGTGAC CGTACCATGC AGGGGGCGAG AGACGTTGCC
    CACAGCATCA TCTTCGAAGT GAAGCTTCCA GAAATGGCAT TTAGCCCAGA TTGTCCTAAA
    GCAGTTGGAT GGGAAAAGAA CCAGAAAGGA GGCATGGGAC CAAGGATGGT GAACCTCAGT
    GAATGTATGG ACCCTAAAAG GTTAGCTGAG TCATCAGTGG ATCTAAATCT CAAACTGATG
    TGTTGGAGAT TGGTTCCTAC TTTAGACTTG GACAAGGTTG TGTCTGTCAA ATGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGCAATGTA GCTAGGACGT TGATGGGTTG GGGCGTGAGA
    CACATCACAT TTGTGGACAA TGCCAAGATC TCCTACTCCA ATCCTGTGAG GCAGCCTCTC
    TATGAGTTTG AAGATTGCCT AGGGGGTGGT AAGCCCAAGG CTCTGGCAGC AGCGGACCGG
    CTCCAGAAAA TATTCCCCGG TGTGAATGCC AGAGGATTCA ACATGAGCAT ACCTATGCCT
    GGGCATCCAG TGAACTTCTC CAGTGTCACT CTGGAGCAAG CCCGCAGAGA TGTGGAGCAA
    CTGGAGCAGC TCATCGAAAG CCATGATGTC GTCTTCCTAT TGATGGACAC CAGGGAGAGC
    CGGTGGCTTC CTGCCGTCAT TGCTGCAAGC AAGAGAAAGC TGGTCATCAA TGCTGCTTTG
    GGATTTGACA CATTTGTTGT CATGAGACAT GGTCTGAAGA AACCAAAGCA GCAAGGAGCT
    GGGGACTTGT GTCCAAACCA CCCTGTGGCA TCTGCTGACC TCCTGGGCTC ATCGCTTTTT
    GCCAACATCC CTGGTTACAA GCTTGGCTGC TACTTCTGCA ATGATGTGGT GGCCCCAGGA
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGTCGTCC AGGACTGGCC
    GTGATTGCAG GAGCCCTGGC CGTGGAATTG ATGGTATCTG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAT CGGATGAATG AGCCTCCAAC CTCTCTTGGG
    CTTGTGCCTC ACCAGATCCG GGGATTTCTT TCACGGTTTG ATAATGTCCT TCCCGTCAGC
    CTGGCATTTG ACAAATGTAC AGCTTGTTCT TCCAAAGTTC TTGATCAATA TGAACGAGAA
    GGATTTAACT TCCTAGCCAA GGTGTTTAAT TCTTCACATT CCTTCTTAGA AGACTTGACT
    GGTCTTACAT TGCTGCATCA AGAAACCCAA GCTGCTGAGG CAGAATATGA AATTCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016861037.1
    CDS61..2160
    Translation

    Target ORF information:

    RefSeq Version XM_017005548.1
    Organism Homo sapiens(human)
    Definition Homo sapiens autophagy related 7 (ATG7), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017005548.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGCGGCAG CTACGGGGGA TCCTGGACTC TCTAAACTGC AGTTTGCCCC TTTTAGTAGT 
    GCCTTGGATG TTGGGTTTTG GCATGAGTTG ACCCAGAAGA AGCTGAACGA GTATCGGCTG
    GATGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAATG GTGACTCTGC TGGGCTGCCA
    GCTCGCTTAA CATTGGAGTT CAGTGCTTTT GACATGAGTG CTCCCACCCC AGCCCGTTGC
    TGCCCAGCTA TTGGAACACT GTATAACACC AACACACTCG AGTCTTTCAA GACTGCAGAT
    AAGAAGCTCC TTTTGGAACA AGCAGCAAAT GAGATATGGG AATCCATAAA ATCAGGCACT
    GCTCTTGAAA ACCCTGTACT CCTCAACAAG TTCCTCCTCT TGACATTTGC AGATCTAAAG
    AAGTACCACT TCTACTATTG GTTTTGCTAT CCTGCCCTCT GTCTTCCAGA GAGTTTACCT
    CTCATTCAGG GGCCAGTGGG TTTGGATCAA AGGTTTTCAC TAAAACAGAT TGAAGCACTA
    GAGTGTGCAT ATGATAATCT TTGTCAAACA GAAGGAGTCA CAGCTCTTCC TTACTTCTTA
    ATCAAGTATG ATGAGAACAT GGTGCTGGTT TCCTTGCTTA AACACTACAG TGATTTCTTC
    CAAGGTCAAA GGACGAAGAT AACAATTGGT GTATATGATC CCTGTAACTT AGCCCAGTAC
    CCTGGATGGC CTTTGAGGAA TTTTTTGGTC CTAGCAGCCC ACAGATGGAG TAGCAGTTTC
    CAGTCTGTTG AAGTTGTTTG CTTCCGTGAC CGTACCATGC AGGGGGCGAG AGACGTTGCC
    CACAGCATCA TCTTCGAAGT GAAGCTTCCA GAAATGGCAT TTAGCCCAGA TTGTCCTAAA
    GCAGTTGGAT GGGAAAAGAA CCAGAAAGGA GGCATGGGAC CAAGGATGGT GAACCTCAGT
    GAATGTATGG ACCCTAAAAG GTTAGCTGAG TCATCAGTGG ATCTAAATCT CAAACTGATG
    TGTTGGAGAT TGGTTCCTAC TTTAGACTTG GACAAGGTTG TGTCTGTCAA ATGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGCAATGTA GCTAGGACGT TGATGGGTTG GGGCGTGAGA
    CACATCACAT TTGTGGACAA TGCCAAGATC TCCTACTCCA ATCCTGTGAG GCAGCCTCTC
    TATGAGTTTG AAGATTGCCT AGGGGGTGGT AAGCCCAAGG CTCTGGCAGC AGCGGACCGG
    CTCCAGAAAA TATTCCCCGG TGTGAATGCC AGAGGATTCA ACATGAGCAT ACCTATGCCT
    GGGCATCCAG TGAACTTCTC CAGTGTCACT CTGGAGCAAG CCCGCAGAGA TGTGGAGCAA
    CTGGAGCAGC TCATCGAAAG CCATGATGTC GTCTTCCTAT TGATGGACAC CAGGGAGAGC
    CGGTGGCTTC CTGCCGTCAT TGCTGCAAGC AAGAGAAAGC TGGTCATCAA TGCTGCTTTG
    GGATTTGACA CATTTGTTGT CATGAGACAT GGTCTGAAGA AACCAAAGCA GCAAGGAGCT
    GGGGACTTGT GTCCAAACCA CCCTGTGGCA TCTGCTGACC TCCTGGGCTC ATCGCTTTTT
    GCCAACATCC CTGGTTACAA GCTTGGCTGC TACTTCTGCA ATGATGTGGT GGCCCCAGGA
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGTCGTCC AGGACTGGCC
    GTGATTGCAG GAGCCCTGGC CGTGGAATTG ATGGTATCTG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAT CGGATGAATG AGCCTCCAAC CTCTCTTGGG
    CTTGTGCCTC ACCAGATCCG GGGATTTCTT TCACGGTTTG ATAATGTCCT TCCCGTCAGC
    CTGGCATTTG ACAAATGTAC AGCTTGTTCT TCCAAAGTTC TTGATCAATA TGAACGAGAA
    GGATTTAACT TCCTAGCCAA GGTGTTTAAT TCTTCACATT CCTTCTTAGA AGACTTGACT
    GGTCTTACAT TGCTGCATCA AGAAACCCAA GCTGCTGAGG CAGAATATGA AATTCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu36917
    Clone ID Related Accession (Same CDS sequence) XM_017005549.1 , XM_011533285.3 , XM_011533285.2 , XM_017005549.2
    Accession Version XM_017005549.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2058bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product ubiquitin-like modifier-activating enzyme ATG7 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGCGGCAG CTACGGGGGA TCCTGGACTC TCTAAACTGC AGTTTGCCCC TTTTAGTAGT 
    GCCTTGGATG TTGGGTTTTG GCATGAGTTG ACCCAGAAGA AGCTGAACGA GTATCGGCTG
    GATGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAATG GTGACTCTGC TGGGCTGCCA
    GCTCGCTTAA CATTGGAGTT CAGTGCTTTT GACATGAGTG CTCCCACCCC AGCCCGTTGC
    TGCCCAGCTA TTGGAACACT GTATAACACC AACACACTCG AGTCTTTCAA GACTGCAGAT
    AAGAAGCTCC TTTTGGAACA AGCAGCAAAT GAGATATGGG AATCCATAAA ATCAGGCACT
    GCTCTTGAAA ACCCTGTACT CCTCAACAAG TTCCTCCTCT TGACATTTGC AGATCTAAAG
    AAGTACCACT TCTACTATTG GTTTTGCTAT CCTGCCCTCT GTCTTCCAGA GAGTTTACCT
    CTCATTCAGG GGCCAGTGGG TTTGGATCAA AGGTTTTCAC TAAAACAGAT TGAAGCACTA
    GAGTGTGCAT ATGATAATCT TTGTCAAACA GAAGGAGTCA CAGCTCTTCC TTACTTCTTA
    ATCAAGTATG ATGAGAACAT GGTGCTGGTT TCCTTGCTTA AACACTACAG TGATTTCTTC
    CAAGGTCAAA GGACGAAGAT AACAATTGGT GTATATGATC CCTGTAACTT AGCCCAGTAC
    CCTGGATGGC CTTTGAGGAA TTTTTTGGTC CTAGCAGCCC ACAGATGGAG TAGCAGTTTC
    CAGTCTGTTG AAGTTGTTTG CTTCCGTGAC CGTACCATGC AGGGGGCGAG AGACGTTGCC
    CACAGCATCA TCTTCGAAGT GAAGCTTCCA GAAATGGCAT TTAGCCCAGA TTGTCCTAAA
    GCAGTTGGAT GGGAAAAGAA CCAGAAAGGA GGCATGGGAC CAAGGATGGT GAACCTCAGT
    GAATGTATGG ACCCTAAAAG GTTAGCTGAG TCATCAGTGG ATCTAAATCT CAAACTGATG
    TGTTGGAGAT TGGTTCCTAC TTTAGACTTG GACAAGGTTG TGTCTGTCAA ATGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGCAATGTA GCTAGGACGT TGATGAATGC CAGAGGATTC
    AACATGAGCA TACCTATGCC TGGGCATCCA GTGAACTTCT CCAGTGTCAC TCTGGAGCAA
    GCCCGCAGAG ATGTGGAGCA ACTGGAGCAG CTCATCGAAA GCCATGATGT CGTCTTCCTA
    TTGATGGACA CCAGGGAGAG CCGGTGGCTT CCTGCCGTCA TTGCTGCAAG CAAGAGAAAG
    CTGGTCATCA ATGCTGCTTT GGGATTTGAC ACATTTGTTG TCATGAGACA TGGTCTGAAG
    AAACCAAAGC AGCAAGGAGC TGGGGACTTG TGTCCAAACC ACCCTGTGGC ATCTGCTGAC
    CTCCTGGGCT CATCGCTTTT TGCCAACATC CCTGGTTACA AGCTTGGCTG CTACTTCTGC
    AATGATGTGG TGGCCCCAGG AGATTCAACC AGAGACCGGA CCTTGGACCA GCAGTGCACT
    GTGAGTCGTC CAGGACTGGC CGTGATTGCA GGAGCCCTGG CCGTGGAATT GATGGTATCT
    GTTTTGCAGC ATCCAGAAGG GGGCTATGCC ATTGCCAGCA GCAGTGACGA TCGGATGAAT
    GAGCCTCCAA CCTCTCTTGG GCTTGTGCCT CACCAGATCC GGGGATTTCT TTCACGGTTT
    GATAATGTCC TTCCCGTCAG CCTGGCATTT GACAAATGTA CAGCTTGTTC TTCCAAAGTT
    CTTGATCAAT ATGAACGAGA AGGATTTAAC TTCCTAGCCA AGGTGTTTAA TTCTTCACAT
    TCCTTCTTAG AAGACTTGAC TGGTCTTACA TTGCTGCATC AAGAAACCCA AGCTGCTGAG
    GATCATCTTT CTTTCATCCG GCCCAAGAAT GGCACCTTCC CCGCCCATCA GGCCACCTGC
    ACTCAGGAGT CGGCTGCCTG TCCTGAAATA GATCTCTACC AGCTCTCAAG CAGAACAATT
    CCTTGCATTC ATACTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016861038.1
    CDS332..2389
    Translation

    Target ORF information:

    RefSeq Version XM_017005549.1
    Organism Homo sapiens(human)
    Definition Homo sapiens autophagy related 7 (ATG7), transcript variant X22, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017005549.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGCGGCAG CTACGGGGGA TCCTGGACTC TCTAAACTGC AGTTTGCCCC TTTTAGTAGT 
    GCCTTGGATG TTGGGTTTTG GCATGAGTTG ACCCAGAAGA AGCTGAACGA GTATCGGCTG
    GATGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAATG GTGACTCTGC TGGGCTGCCA
    GCTCGCTTAA CATTGGAGTT CAGTGCTTTT GACATGAGTG CTCCCACCCC AGCCCGTTGC
    TGCCCAGCTA TTGGAACACT GTATAACACC AACACACTCG AGTCTTTCAA GACTGCAGAT
    AAGAAGCTCC TTTTGGAACA AGCAGCAAAT GAGATATGGG AATCCATAAA ATCAGGCACT
    GCTCTTGAAA ACCCTGTACT CCTCAACAAG TTCCTCCTCT TGACATTTGC AGATCTAAAG
    AAGTACCACT TCTACTATTG GTTTTGCTAT CCTGCCCTCT GTCTTCCAGA GAGTTTACCT
    CTCATTCAGG GGCCAGTGGG TTTGGATCAA AGGTTTTCAC TAAAACAGAT TGAAGCACTA
    GAGTGTGCAT ATGATAATCT TTGTCAAACA GAAGGAGTCA CAGCTCTTCC TTACTTCTTA
    ATCAAGTATG ATGAGAACAT GGTGCTGGTT TCCTTGCTTA AACACTACAG TGATTTCTTC
    CAAGGTCAAA GGACGAAGAT AACAATTGGT GTATATGATC CCTGTAACTT AGCCCAGTAC
    CCTGGATGGC CTTTGAGGAA TTTTTTGGTC CTAGCAGCCC ACAGATGGAG TAGCAGTTTC
    CAGTCTGTTG AAGTTGTTTG CTTCCGTGAC CGTACCATGC AGGGGGCGAG AGACGTTGCC
    CACAGCATCA TCTTCGAAGT GAAGCTTCCA GAAATGGCAT TTAGCCCAGA TTGTCCTAAA
    GCAGTTGGAT GGGAAAAGAA CCAGAAAGGA GGCATGGGAC CAAGGATGGT GAACCTCAGT
    GAATGTATGG ACCCTAAAAG GTTAGCTGAG TCATCAGTGG ATCTAAATCT CAAACTGATG
    TGTTGGAGAT TGGTTCCTAC TTTAGACTTG GACAAGGTTG TGTCTGTCAA ATGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGCAATGTA GCTAGGACGT TGATGAATGC CAGAGGATTC
    AACATGAGCA TACCTATGCC TGGGCATCCA GTGAACTTCT CCAGTGTCAC TCTGGAGCAA
    GCCCGCAGAG ATGTGGAGCA ACTGGAGCAG CTCATCGAAA GCCATGATGT CGTCTTCCTA
    TTGATGGACA CCAGGGAGAG CCGGTGGCTT CCTGCCGTCA TTGCTGCAAG CAAGAGAAAG
    CTGGTCATCA ATGCTGCTTT GGGATTTGAC ACATTTGTTG TCATGAGACA TGGTCTGAAG
    AAACCAAAGC AGCAAGGAGC TGGGGACTTG TGTCCAAACC ACCCTGTGGC ATCTGCTGAC
    CTCCTGGGCT CATCGCTTTT TGCCAACATC CCTGGTTACA AGCTTGGCTG CTACTTCTGC
    AATGATGTGG TGGCCCCAGG AGATTCAACC AGAGACCGGA CCTTGGACCA GCAGTGCACT
    GTGAGTCGTC CAGGACTGGC CGTGATTGCA GGAGCCCTGG CCGTGGAATT GATGGTATCT
    GTTTTGCAGC ATCCAGAAGG GGGCTATGCC ATTGCCAGCA GCAGTGACGA TCGGATGAAT
    GAGCCTCCAA CCTCTCTTGG GCTTGTGCCT CACCAGATCC GGGGATTTCT TTCACGGTTT
    GATAATGTCC TTCCCGTCAG CCTGGCATTT GACAAATGTA CAGCTTGTTC TTCCAAAGTT
    CTTGATCAAT ATGAACGAGA AGGATTTAAC TTCCTAGCCA AGGTGTTTAA TTCTTCACAT
    TCCTTCTTAG AAGACTTGAC TGGTCTTACA TTGCTGCATC AAGAAACCCA AGCTGCTGAG
    GATCATCTTT CTTTCATCCG GCCCAAGAAT GGCACCTTCC CCGCCCATCA GGCCACCTGC
    ACTCAGGAGT CGGCTGCCTG TCCTGAAATA GATCTCTACC AGCTCTCAAG CAGAACAATT
    CCTTGCATTC ATACTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu36917
    Clone ID Related Accession (Same CDS sequence) XM_017005549.1 , XM_011533285.3 , XM_011533285.2 , XM_017005549.2
    Accession Version XM_011533285.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2058bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product ubiquitin-like modifier-activating enzyme ATG7 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011533285.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGCGGCAG CTACGGGGGA TCCTGGACTC TCTAAACTGC AGTTTGCCCC TTTTAGTAGT 
    GCCTTGGATG TTGGGTTTTG GCATGAGTTG ACCCAGAAGA AGCTGAACGA GTATCGGCTG
    GATGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAATG GTGACTCTGC TGGGCTGCCA
    GCTCGCTTAA CATTGGAGTT CAGTGCTTTT GACATGAGTG CTCCCACCCC AGCCCGTTGC
    TGCCCAGCTA TTGGAACACT GTATAACACC AACACACTCG AGTCTTTCAA GACTGCAGAT
    AAGAAGCTCC TTTTGGAACA AGCAGCAAAT GAGATATGGG AATCCATAAA ATCAGGCACT
    GCTCTTGAAA ACCCTGTACT CCTCAACAAG TTCCTCCTCT TGACATTTGC AGATCTAAAG
    AAGTACCACT TCTACTATTG GTTTTGCTAT CCTGCCCTCT GTCTTCCAGA GAGTTTACCT
    CTCATTCAGG GGCCAGTGGG TTTGGATCAA AGGTTTTCAC TAAAACAGAT TGAAGCACTA
    GAGTGTGCAT ATGATAATCT TTGTCAAACA GAAGGAGTCA CAGCTCTTCC TTACTTCTTA
    ATCAAGTATG ATGAGAACAT GGTGCTGGTT TCCTTGCTTA AACACTACAG TGATTTCTTC
    CAAGGTCAAA GGACGAAGAT AACAATTGGT GTATATGATC CCTGTAACTT AGCCCAGTAC
    CCTGGATGGC CTTTGAGGAA TTTTTTGGTC CTAGCAGCCC ACAGATGGAG TAGCAGTTTC
    CAGTCTGTTG AAGTTGTTTG CTTCCGTGAC CGTACCATGC AGGGGGCGAG AGACGTTGCC
    CACAGCATCA TCTTCGAAGT GAAGCTTCCA GAAATGGCAT TTAGCCCAGA TTGTCCTAAA
    GCAGTTGGAT GGGAAAAGAA CCAGAAAGGA GGCATGGGAC CAAGGATGGT GAACCTCAGT
    GAATGTATGG ACCCTAAAAG GTTAGCTGAG TCATCAGTGG ATCTAAATCT CAAACTGATG
    TGTTGGAGAT TGGTTCCTAC TTTAGACTTG GACAAGGTTG TGTCTGTCAA ATGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGCAATGTA GCTAGGACGT TGATGAATGC CAGAGGATTC
    AACATGAGCA TACCTATGCC TGGGCATCCA GTGAACTTCT CCAGTGTCAC TCTGGAGCAA
    GCCCGCAGAG ATGTGGAGCA ACTGGAGCAG CTCATCGAAA GCCATGATGT CGTCTTCCTA
    TTGATGGACA CCAGGGAGAG CCGGTGGCTT CCTGCCGTCA TTGCTGCAAG CAAGAGAAAG
    CTGGTCATCA ATGCTGCTTT GGGATTTGAC ACATTTGTTG TCATGAGACA TGGTCTGAAG
    AAACCAAAGC AGCAAGGAGC TGGGGACTTG TGTCCAAACC ACCCTGTGGC ATCTGCTGAC
    CTCCTGGGCT CATCGCTTTT TGCCAACATC CCTGGTTACA AGCTTGGCTG CTACTTCTGC
    AATGATGTGG TGGCCCCAGG AGATTCAACC AGAGACCGGA CCTTGGACCA GCAGTGCACT
    GTGAGTCGTC CAGGACTGGC CGTGATTGCA GGAGCCCTGG CCGTGGAATT GATGGTATCT
    GTTTTGCAGC ATCCAGAAGG GGGCTATGCC ATTGCCAGCA GCAGTGACGA TCGGATGAAT
    GAGCCTCCAA CCTCTCTTGG GCTTGTGCCT CACCAGATCC GGGGATTTCT TTCACGGTTT
    GATAATGTCC TTCCCGTCAG CCTGGCATTT GACAAATGTA CAGCTTGTTC TTCCAAAGTT
    CTTGATCAAT ATGAACGAGA AGGATTTAAC TTCCTAGCCA AGGTGTTTAA TTCTTCACAT
    TCCTTCTTAG AAGACTTGAC TGGTCTTACA TTGCTGCATC AAGAAACCCA AGCTGCTGAG
    GATCATCTTT CTTTCATCCG GCCCAAGAAT GGCACCTTCC CCGCCCATCA GGCCACCTGC
    ACTCAGGAGT CGGCTGCCTG TCCTGAAATA GATCTCTACC AGCTCTCAAG CAGAACAATT
    CCTTGCATTC ATACTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011531587.1
    CDS449..2506
    Translation

    Target ORF information:

    RefSeq Version XM_011533285.3
    Organism Homo sapiens(human)
    Definition Homo sapiens autophagy related 7 (ATG7), transcript variant X18, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011533285.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGCGGCAG CTACGGGGGA TCCTGGACTC TCTAAACTGC AGTTTGCCCC TTTTAGTAGT 
    GCCTTGGATG TTGGGTTTTG GCATGAGTTG ACCCAGAAGA AGCTGAACGA GTATCGGCTG
    GATGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAATG GTGACTCTGC TGGGCTGCCA
    GCTCGCTTAA CATTGGAGTT CAGTGCTTTT GACATGAGTG CTCCCACCCC AGCCCGTTGC
    TGCCCAGCTA TTGGAACACT GTATAACACC AACACACTCG AGTCTTTCAA GACTGCAGAT
    AAGAAGCTCC TTTTGGAACA AGCAGCAAAT GAGATATGGG AATCCATAAA ATCAGGCACT
    GCTCTTGAAA ACCCTGTACT CCTCAACAAG TTCCTCCTCT TGACATTTGC AGATCTAAAG
    AAGTACCACT TCTACTATTG GTTTTGCTAT CCTGCCCTCT GTCTTCCAGA GAGTTTACCT
    CTCATTCAGG GGCCAGTGGG TTTGGATCAA AGGTTTTCAC TAAAACAGAT TGAAGCACTA
    GAGTGTGCAT ATGATAATCT TTGTCAAACA GAAGGAGTCA CAGCTCTTCC TTACTTCTTA
    ATCAAGTATG ATGAGAACAT GGTGCTGGTT TCCTTGCTTA AACACTACAG TGATTTCTTC
    CAAGGTCAAA GGACGAAGAT AACAATTGGT GTATATGATC CCTGTAACTT AGCCCAGTAC
    CCTGGATGGC CTTTGAGGAA TTTTTTGGTC CTAGCAGCCC ACAGATGGAG TAGCAGTTTC
    CAGTCTGTTG AAGTTGTTTG CTTCCGTGAC CGTACCATGC AGGGGGCGAG AGACGTTGCC
    CACAGCATCA TCTTCGAAGT GAAGCTTCCA GAAATGGCAT TTAGCCCAGA TTGTCCTAAA
    GCAGTTGGAT GGGAAAAGAA CCAGAAAGGA GGCATGGGAC CAAGGATGGT GAACCTCAGT
    GAATGTATGG ACCCTAAAAG GTTAGCTGAG TCATCAGTGG ATCTAAATCT CAAACTGATG
    TGTTGGAGAT TGGTTCCTAC TTTAGACTTG GACAAGGTTG TGTCTGTCAA ATGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGCAATGTA GCTAGGACGT TGATGAATGC CAGAGGATTC
    AACATGAGCA TACCTATGCC TGGGCATCCA GTGAACTTCT CCAGTGTCAC TCTGGAGCAA
    GCCCGCAGAG ATGTGGAGCA ACTGGAGCAG CTCATCGAAA GCCATGATGT CGTCTTCCTA
    TTGATGGACA CCAGGGAGAG CCGGTGGCTT CCTGCCGTCA TTGCTGCAAG CAAGAGAAAG
    CTGGTCATCA ATGCTGCTTT GGGATTTGAC ACATTTGTTG TCATGAGACA TGGTCTGAAG
    AAACCAAAGC AGCAAGGAGC TGGGGACTTG TGTCCAAACC ACCCTGTGGC ATCTGCTGAC
    CTCCTGGGCT CATCGCTTTT TGCCAACATC CCTGGTTACA AGCTTGGCTG CTACTTCTGC
    AATGATGTGG TGGCCCCAGG AGATTCAACC AGAGACCGGA CCTTGGACCA GCAGTGCACT
    GTGAGTCGTC CAGGACTGGC CGTGATTGCA GGAGCCCTGG CCGTGGAATT GATGGTATCT
    GTTTTGCAGC ATCCAGAAGG GGGCTATGCC ATTGCCAGCA GCAGTGACGA TCGGATGAAT
    GAGCCTCCAA CCTCTCTTGG GCTTGTGCCT CACCAGATCC GGGGATTTCT TTCACGGTTT
    GATAATGTCC TTCCCGTCAG CCTGGCATTT GACAAATGTA CAGCTTGTTC TTCCAAAGTT
    CTTGATCAAT ATGAACGAGA AGGATTTAAC TTCCTAGCCA AGGTGTTTAA TTCTTCACAT
    TCCTTCTTAG AAGACTTGAC TGGTCTTACA TTGCTGCATC AAGAAACCCA AGCTGCTGAG
    GATCATCTTT CTTTCATCCG GCCCAAGAAT GGCACCTTCC CCGCCCATCA GGCCACCTGC
    ACTCAGGAGT CGGCTGCCTG TCCTGAAATA GATCTCTACC AGCTCTCAAG CAGAACAATT
    CCTTGCATTC ATACTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu36917
    Clone ID Related Accession (Same CDS sequence) XM_017005549.1 , XM_011533285.3 , XM_011533285.2 , XM_017005549.2
    Accession Version XM_011533285.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2058bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product ubiquitin-like modifier-activating enzyme ATG7 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011533285.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGCGGCAG CTACGGGGGA TCCTGGACTC TCTAAACTGC AGTTTGCCCC TTTTAGTAGT 
    GCCTTGGATG TTGGGTTTTG GCATGAGTTG ACCCAGAAGA AGCTGAACGA GTATCGGCTG
    GATGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAATG GTGACTCTGC TGGGCTGCCA
    GCTCGCTTAA CATTGGAGTT CAGTGCTTTT GACATGAGTG CTCCCACCCC AGCCCGTTGC
    TGCCCAGCTA TTGGAACACT GTATAACACC AACACACTCG AGTCTTTCAA GACTGCAGAT
    AAGAAGCTCC TTTTGGAACA AGCAGCAAAT GAGATATGGG AATCCATAAA ATCAGGCACT
    GCTCTTGAAA ACCCTGTACT CCTCAACAAG TTCCTCCTCT TGACATTTGC AGATCTAAAG
    AAGTACCACT TCTACTATTG GTTTTGCTAT CCTGCCCTCT GTCTTCCAGA GAGTTTACCT
    CTCATTCAGG GGCCAGTGGG TTTGGATCAA AGGTTTTCAC TAAAACAGAT TGAAGCACTA
    GAGTGTGCAT ATGATAATCT TTGTCAAACA GAAGGAGTCA CAGCTCTTCC TTACTTCTTA
    ATCAAGTATG ATGAGAACAT GGTGCTGGTT TCCTTGCTTA AACACTACAG TGATTTCTTC
    CAAGGTCAAA GGACGAAGAT AACAATTGGT GTATATGATC CCTGTAACTT AGCCCAGTAC
    CCTGGATGGC CTTTGAGGAA TTTTTTGGTC CTAGCAGCCC ACAGATGGAG TAGCAGTTTC
    CAGTCTGTTG AAGTTGTTTG CTTCCGTGAC CGTACCATGC AGGGGGCGAG AGACGTTGCC
    CACAGCATCA TCTTCGAAGT GAAGCTTCCA GAAATGGCAT TTAGCCCAGA TTGTCCTAAA
    GCAGTTGGAT GGGAAAAGAA CCAGAAAGGA GGCATGGGAC CAAGGATGGT GAACCTCAGT
    GAATGTATGG ACCCTAAAAG GTTAGCTGAG TCATCAGTGG ATCTAAATCT CAAACTGATG
    TGTTGGAGAT TGGTTCCTAC TTTAGACTTG GACAAGGTTG TGTCTGTCAA ATGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGCAATGTA GCTAGGACGT TGATGAATGC CAGAGGATTC
    AACATGAGCA TACCTATGCC TGGGCATCCA GTGAACTTCT CCAGTGTCAC TCTGGAGCAA
    GCCCGCAGAG ATGTGGAGCA ACTGGAGCAG CTCATCGAAA GCCATGATGT CGTCTTCCTA
    TTGATGGACA CCAGGGAGAG CCGGTGGCTT CCTGCCGTCA TTGCTGCAAG CAAGAGAAAG
    CTGGTCATCA ATGCTGCTTT GGGATTTGAC ACATTTGTTG TCATGAGACA TGGTCTGAAG
    AAACCAAAGC AGCAAGGAGC TGGGGACTTG TGTCCAAACC ACCCTGTGGC ATCTGCTGAC
    CTCCTGGGCT CATCGCTTTT TGCCAACATC CCTGGTTACA AGCTTGGCTG CTACTTCTGC
    AATGATGTGG TGGCCCCAGG AGATTCAACC AGAGACCGGA CCTTGGACCA GCAGTGCACT
    GTGAGTCGTC CAGGACTGGC CGTGATTGCA GGAGCCCTGG CCGTGGAATT GATGGTATCT
    GTTTTGCAGC ATCCAGAAGG GGGCTATGCC ATTGCCAGCA GCAGTGACGA TCGGATGAAT
    GAGCCTCCAA CCTCTCTTGG GCTTGTGCCT CACCAGATCC GGGGATTTCT TTCACGGTTT
    GATAATGTCC TTCCCGTCAG CCTGGCATTT GACAAATGTA CAGCTTGTTC TTCCAAAGTT
    CTTGATCAAT ATGAACGAGA AGGATTTAAC TTCCTAGCCA AGGTGTTTAA TTCTTCACAT
    TCCTTCTTAG AAGACTTGAC TGGTCTTACA TTGCTGCATC AAGAAACCCA AGCTGCTGAG
    GATCATCTTT CTTTCATCCG GCCCAAGAAT GGCACCTTCC CCGCCCATCA GGCCACCTGC
    ACTCAGGAGT CGGCTGCCTG TCCTGAAATA GATCTCTACC AGCTCTCAAG CAGAACAATT
    CCTTGCATTC ATACTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011531587.1
    CDS449..2506
    Translation

    Target ORF information:

    RefSeq Version XM_011533285.2
    Organism Homo sapiens(human)
    Definition Homo sapiens autophagy related 7 (ATG7), transcript variant X21, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011533285.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGCGGCAG CTACGGGGGA TCCTGGACTC TCTAAACTGC AGTTTGCCCC TTTTAGTAGT 
    GCCTTGGATG TTGGGTTTTG GCATGAGTTG ACCCAGAAGA AGCTGAACGA GTATCGGCTG
    GATGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAATG GTGACTCTGC TGGGCTGCCA
    GCTCGCTTAA CATTGGAGTT CAGTGCTTTT GACATGAGTG CTCCCACCCC AGCCCGTTGC
    TGCCCAGCTA TTGGAACACT GTATAACACC AACACACTCG AGTCTTTCAA GACTGCAGAT
    AAGAAGCTCC TTTTGGAACA AGCAGCAAAT GAGATATGGG AATCCATAAA ATCAGGCACT
    GCTCTTGAAA ACCCTGTACT CCTCAACAAG TTCCTCCTCT TGACATTTGC AGATCTAAAG
    AAGTACCACT TCTACTATTG GTTTTGCTAT CCTGCCCTCT GTCTTCCAGA GAGTTTACCT
    CTCATTCAGG GGCCAGTGGG TTTGGATCAA AGGTTTTCAC TAAAACAGAT TGAAGCACTA
    GAGTGTGCAT ATGATAATCT TTGTCAAACA GAAGGAGTCA CAGCTCTTCC TTACTTCTTA
    ATCAAGTATG ATGAGAACAT GGTGCTGGTT TCCTTGCTTA AACACTACAG TGATTTCTTC
    CAAGGTCAAA GGACGAAGAT AACAATTGGT GTATATGATC CCTGTAACTT AGCCCAGTAC
    CCTGGATGGC CTTTGAGGAA TTTTTTGGTC CTAGCAGCCC ACAGATGGAG TAGCAGTTTC
    CAGTCTGTTG AAGTTGTTTG CTTCCGTGAC CGTACCATGC AGGGGGCGAG AGACGTTGCC
    CACAGCATCA TCTTCGAAGT GAAGCTTCCA GAAATGGCAT TTAGCCCAGA TTGTCCTAAA
    GCAGTTGGAT GGGAAAAGAA CCAGAAAGGA GGCATGGGAC CAAGGATGGT GAACCTCAGT
    GAATGTATGG ACCCTAAAAG GTTAGCTGAG TCATCAGTGG ATCTAAATCT CAAACTGATG
    TGTTGGAGAT TGGTTCCTAC TTTAGACTTG GACAAGGTTG TGTCTGTCAA ATGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGCAATGTA GCTAGGACGT TGATGAATGC CAGAGGATTC
    AACATGAGCA TACCTATGCC TGGGCATCCA GTGAACTTCT CCAGTGTCAC TCTGGAGCAA
    GCCCGCAGAG ATGTGGAGCA ACTGGAGCAG CTCATCGAAA GCCATGATGT CGTCTTCCTA
    TTGATGGACA CCAGGGAGAG CCGGTGGCTT CCTGCCGTCA TTGCTGCAAG CAAGAGAAAG
    CTGGTCATCA ATGCTGCTTT GGGATTTGAC ACATTTGTTG TCATGAGACA TGGTCTGAAG
    AAACCAAAGC AGCAAGGAGC TGGGGACTTG TGTCCAAACC ACCCTGTGGC ATCTGCTGAC
    CTCCTGGGCT CATCGCTTTT TGCCAACATC CCTGGTTACA AGCTTGGCTG CTACTTCTGC
    AATGATGTGG TGGCCCCAGG AGATTCAACC AGAGACCGGA CCTTGGACCA GCAGTGCACT
    GTGAGTCGTC CAGGACTGGC CGTGATTGCA GGAGCCCTGG CCGTGGAATT GATGGTATCT
    GTTTTGCAGC ATCCAGAAGG GGGCTATGCC ATTGCCAGCA GCAGTGACGA TCGGATGAAT
    GAGCCTCCAA CCTCTCTTGG GCTTGTGCCT CACCAGATCC GGGGATTTCT TTCACGGTTT
    GATAATGTCC TTCCCGTCAG CCTGGCATTT GACAAATGTA CAGCTTGTTC TTCCAAAGTT
    CTTGATCAAT ATGAACGAGA AGGATTTAAC TTCCTAGCCA AGGTGTTTAA TTCTTCACAT
    TCCTTCTTAG AAGACTTGAC TGGTCTTACA TTGCTGCATC AAGAAACCCA AGCTGCTGAG
    GATCATCTTT CTTTCATCCG GCCCAAGAAT GGCACCTTCC CCGCCCATCA GGCCACCTGC
    ACTCAGGAGT CGGCTGCCTG TCCTGAAATA GATCTCTACC AGCTCTCAAG CAGAACAATT
    CCTTGCATTC ATACTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu36917
    Clone ID Related Accession (Same CDS sequence) XM_017005549.1 , XM_011533285.3 , XM_011533285.2 , XM_017005549.2
    Accession Version XM_017005549.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2058bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product ubiquitin-like modifier-activating enzyme ATG7 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017005549.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGCGGCAG CTACGGGGGA TCCTGGACTC TCTAAACTGC AGTTTGCCCC TTTTAGTAGT 
    GCCTTGGATG TTGGGTTTTG GCATGAGTTG ACCCAGAAGA AGCTGAACGA GTATCGGCTG
    GATGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAATG GTGACTCTGC TGGGCTGCCA
    GCTCGCTTAA CATTGGAGTT CAGTGCTTTT GACATGAGTG CTCCCACCCC AGCCCGTTGC
    TGCCCAGCTA TTGGAACACT GTATAACACC AACACACTCG AGTCTTTCAA GACTGCAGAT
    AAGAAGCTCC TTTTGGAACA AGCAGCAAAT GAGATATGGG AATCCATAAA ATCAGGCACT
    GCTCTTGAAA ACCCTGTACT CCTCAACAAG TTCCTCCTCT TGACATTTGC AGATCTAAAG
    AAGTACCACT TCTACTATTG GTTTTGCTAT CCTGCCCTCT GTCTTCCAGA GAGTTTACCT
    CTCATTCAGG GGCCAGTGGG TTTGGATCAA AGGTTTTCAC TAAAACAGAT TGAAGCACTA
    GAGTGTGCAT ATGATAATCT TTGTCAAACA GAAGGAGTCA CAGCTCTTCC TTACTTCTTA
    ATCAAGTATG ATGAGAACAT GGTGCTGGTT TCCTTGCTTA AACACTACAG TGATTTCTTC
    CAAGGTCAAA GGACGAAGAT AACAATTGGT GTATATGATC CCTGTAACTT AGCCCAGTAC
    CCTGGATGGC CTTTGAGGAA TTTTTTGGTC CTAGCAGCCC ACAGATGGAG TAGCAGTTTC
    CAGTCTGTTG AAGTTGTTTG CTTCCGTGAC CGTACCATGC AGGGGGCGAG AGACGTTGCC
    CACAGCATCA TCTTCGAAGT GAAGCTTCCA GAAATGGCAT TTAGCCCAGA TTGTCCTAAA
    GCAGTTGGAT GGGAAAAGAA CCAGAAAGGA GGCATGGGAC CAAGGATGGT GAACCTCAGT
    GAATGTATGG ACCCTAAAAG GTTAGCTGAG TCATCAGTGG ATCTAAATCT CAAACTGATG
    TGTTGGAGAT TGGTTCCTAC TTTAGACTTG GACAAGGTTG TGTCTGTCAA ATGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGCAATGTA GCTAGGACGT TGATGAATGC CAGAGGATTC
    AACATGAGCA TACCTATGCC TGGGCATCCA GTGAACTTCT CCAGTGTCAC TCTGGAGCAA
    GCCCGCAGAG ATGTGGAGCA ACTGGAGCAG CTCATCGAAA GCCATGATGT CGTCTTCCTA
    TTGATGGACA CCAGGGAGAG CCGGTGGCTT CCTGCCGTCA TTGCTGCAAG CAAGAGAAAG
    CTGGTCATCA ATGCTGCTTT GGGATTTGAC ACATTTGTTG TCATGAGACA TGGTCTGAAG
    AAACCAAAGC AGCAAGGAGC TGGGGACTTG TGTCCAAACC ACCCTGTGGC ATCTGCTGAC
    CTCCTGGGCT CATCGCTTTT TGCCAACATC CCTGGTTACA AGCTTGGCTG CTACTTCTGC
    AATGATGTGG TGGCCCCAGG AGATTCAACC AGAGACCGGA CCTTGGACCA GCAGTGCACT
    GTGAGTCGTC CAGGACTGGC CGTGATTGCA GGAGCCCTGG CCGTGGAATT GATGGTATCT
    GTTTTGCAGC ATCCAGAAGG GGGCTATGCC ATTGCCAGCA GCAGTGACGA TCGGATGAAT
    GAGCCTCCAA CCTCTCTTGG GCTTGTGCCT CACCAGATCC GGGGATTTCT TTCACGGTTT
    GATAATGTCC TTCCCGTCAG CCTGGCATTT GACAAATGTA CAGCTTGTTC TTCCAAAGTT
    CTTGATCAAT ATGAACGAGA AGGATTTAAC TTCCTAGCCA AGGTGTTTAA TTCTTCACAT
    TCCTTCTTAG AAGACTTGAC TGGTCTTACA TTGCTGCATC AAGAAACCCA AGCTGCTGAG
    GATCATCTTT CTTTCATCCG GCCCAAGAAT GGCACCTTCC CCGCCCATCA GGCCACCTGC
    ACTCAGGAGT CGGCTGCCTG TCCTGAAATA GATCTCTACC AGCTCTCAAG CAGAACAATT
    CCTTGCATTC ATACTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016861038.1
    CDS332..2389
    Translation

    Target ORF information:

    RefSeq Version XM_017005549.2
    Organism Homo sapiens(human)
    Definition Homo sapiens autophagy related 7 (ATG7), transcript variant X19, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017005549.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGCGGCAG CTACGGGGGA TCCTGGACTC TCTAAACTGC AGTTTGCCCC TTTTAGTAGT 
    GCCTTGGATG TTGGGTTTTG GCATGAGTTG ACCCAGAAGA AGCTGAACGA GTATCGGCTG
    GATGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAATG GTGACTCTGC TGGGCTGCCA
    GCTCGCTTAA CATTGGAGTT CAGTGCTTTT GACATGAGTG CTCCCACCCC AGCCCGTTGC
    TGCCCAGCTA TTGGAACACT GTATAACACC AACACACTCG AGTCTTTCAA GACTGCAGAT
    AAGAAGCTCC TTTTGGAACA AGCAGCAAAT GAGATATGGG AATCCATAAA ATCAGGCACT
    GCTCTTGAAA ACCCTGTACT CCTCAACAAG TTCCTCCTCT TGACATTTGC AGATCTAAAG
    AAGTACCACT TCTACTATTG GTTTTGCTAT CCTGCCCTCT GTCTTCCAGA GAGTTTACCT
    CTCATTCAGG GGCCAGTGGG TTTGGATCAA AGGTTTTCAC TAAAACAGAT TGAAGCACTA
    GAGTGTGCAT ATGATAATCT TTGTCAAACA GAAGGAGTCA CAGCTCTTCC TTACTTCTTA
    ATCAAGTATG ATGAGAACAT GGTGCTGGTT TCCTTGCTTA AACACTACAG TGATTTCTTC
    CAAGGTCAAA GGACGAAGAT AACAATTGGT GTATATGATC CCTGTAACTT AGCCCAGTAC
    CCTGGATGGC CTTTGAGGAA TTTTTTGGTC CTAGCAGCCC ACAGATGGAG TAGCAGTTTC
    CAGTCTGTTG AAGTTGTTTG CTTCCGTGAC CGTACCATGC AGGGGGCGAG AGACGTTGCC
    CACAGCATCA TCTTCGAAGT GAAGCTTCCA GAAATGGCAT TTAGCCCAGA TTGTCCTAAA
    GCAGTTGGAT GGGAAAAGAA CCAGAAAGGA GGCATGGGAC CAAGGATGGT GAACCTCAGT
    GAATGTATGG ACCCTAAAAG GTTAGCTGAG TCATCAGTGG ATCTAAATCT CAAACTGATG
    TGTTGGAGAT TGGTTCCTAC TTTAGACTTG GACAAGGTTG TGTCTGTCAA ATGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGCAATGTA GCTAGGACGT TGATGAATGC CAGAGGATTC
    AACATGAGCA TACCTATGCC TGGGCATCCA GTGAACTTCT CCAGTGTCAC TCTGGAGCAA
    GCCCGCAGAG ATGTGGAGCA ACTGGAGCAG CTCATCGAAA GCCATGATGT CGTCTTCCTA
    TTGATGGACA CCAGGGAGAG CCGGTGGCTT CCTGCCGTCA TTGCTGCAAG CAAGAGAAAG
    CTGGTCATCA ATGCTGCTTT GGGATTTGAC ACATTTGTTG TCATGAGACA TGGTCTGAAG
    AAACCAAAGC AGCAAGGAGC TGGGGACTTG TGTCCAAACC ACCCTGTGGC ATCTGCTGAC
    CTCCTGGGCT CATCGCTTTT TGCCAACATC CCTGGTTACA AGCTTGGCTG CTACTTCTGC
    AATGATGTGG TGGCCCCAGG AGATTCAACC AGAGACCGGA CCTTGGACCA GCAGTGCACT
    GTGAGTCGTC CAGGACTGGC CGTGATTGCA GGAGCCCTGG CCGTGGAATT GATGGTATCT
    GTTTTGCAGC ATCCAGAAGG GGGCTATGCC ATTGCCAGCA GCAGTGACGA TCGGATGAAT
    GAGCCTCCAA CCTCTCTTGG GCTTGTGCCT CACCAGATCC GGGGATTTCT TTCACGGTTT
    GATAATGTCC TTCCCGTCAG CCTGGCATTT GACAAATGTA CAGCTTGTTC TTCCAAAGTT
    CTTGATCAAT ATGAACGAGA AGGATTTAAC TTCCTAGCCA AGGTGTTTAA TTCTTCACAT
    TCCTTCTTAG AAGACTTGAC TGGTCTTACA TTGCTGCATC AAGAAACCCA AGCTGCTGAG
    GATCATCTTT CTTTCATCCG GCCCAAGAAT GGCACCTTCC CCGCCCATCA GGCCACCTGC
    ACTCAGGAGT CGGCTGCCTG TCCTGAAATA GATCTCTACC AGCTCTCAAG CAGAACAATT
    CCTTGCATTC ATACTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu64106
    Clone ID Related Accession (Same CDS sequence) XM_011533279.2 , XM_024453312.1
    Accession Version XM_011533279.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2196bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product ubiquitin-like modifier-activating enzyme ATG7 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011533279.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCGGCAG CTACGGGGGA TCCTGGACTC TCTAAACTGC AGTTTGCCCC TTTTAGTAGT 
    GCCTTGGATG TTGGGTTTTG GCATGAGTTG ACCCAGAAGA AGCTGAACGA GTATCGGCTG
    GATGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAATG GTGACTCTGC TGGGCTGCCA
    GCTCGCTTAA CATTGGAGTT CAGTGCTTTT GACATGAGTG CTCCCACCCC AGCCCGTTGC
    TGCCCAGCTA TTGGAACACT GTATAACACC AACACACTCG AGTCTTTCAA GACTGCAGAT
    AAGAAGCTCC TTTTGGAACA AGCAGCAAAT GAGATATGGG AATCCATAAA ATCAGGCACT
    GCTCTTGAAA ACCCTGTACT CCTCAACAAG TTCCTCCTCT TGACATTTGC AGATCTAAAG
    AAGTACCACT TCTACTATTG GTTTTGCTAT CCTGCCCTCT GTCTTCCAGA GAGTTTACCT
    CTCATTCAGG GGCCAGTGGG TTTGGATCAA AGGTTTTCAC TAAAACAGAT TGAAGCACTA
    GAGTGTGCAT ATGATAATCT TTGTCAAACA GAAGGAGTCA CAGCTCTTCC TTACTTCTTA
    ATCAAGTATG ATGAGAACAT GGTGCTGGTT TCCTTGCTTA AACACTACAG TGATTTCTTC
    CAAGGTCAAA GGACGAAGAT AACAATTGGT GTATATGATC CCTGTAACTT AGCCCAGTAC
    CCTGGATGGC CTTTGAGGAA TTTTTTGGTC CTAGCAGCCC ACAGATGGAG TAGCAGTTTC
    CAGTCTGTTG AAGTTGTTTG CTTCCGTGAC CGTACCATGC AGGGGGCGAG AGACGTTGCC
    CACAGCATCA TCTTCGAAGT GAAGCTTCCA GAAATGGCAT TTAGCCCAGA TTGTCCTAAA
    GCAGTTGGAT GGGAAAAGAA CCAGAAAGGA GGCATGGGAC CAAGGATGGT GAACCTCAGT
    GAATGTATGG ACCCTAAAAG GTTAGCTGAG TCATCAGTGG ATCTAAATCT CAAACTGATG
    TGTTGGAGAT TGGTTCCTAC TTTAGACTTG GACAAGGTTG TGTCTGTCAA ATGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGCAATGTA GCTAGGACGT TGATGGGTTG GGGCGTGAGA
    CACATCACAT TTGTGGACAA TGCCAAGATC TCCTACTCCA ATCCTGTGAG GCAGCCTCTC
    TATGAGTTTG AAGATTGCCT AGGGGGTGGT AAGCCCAAGG CTCTGGCAGC AGCGGACCGG
    CTCCAGAAAA TATTCCCCGG TGTGAATGCC AGAGGATTCA ACATGAGCAT ACCTATGCCT
    GGGCATCCAG TGAACTTCTC CAGTGTCACT CTGGAGCAAG CCCGCAGAGA TGTGGAGCAA
    CTGGAGCAGC TCATCGAAAG CCATGATGTC GTCTTCCTAT TGATGGACAC CAGGGAGAGC
    CGGTGGCTTC CTGCCGTCAT TGCTGCAAGC AAGAGAAAGC TGGTCATCAA TGCTGCTTTG
    GGATTTGACA CATTTGTTGT CATGAGACAT GGTCTGAAGA AACCAAAGCA GCAAGGAGCT
    GGGGACTTGT GTCCAAACCA CCCTGTGGCA TCTGCTGACC TCCTGGGCTC ATCGCTTTTT
    GCCAACATCC CTGGTTACAA GCTTGGCTGC TACTTCTGCA ATGATGTGGT GGCCCCAGGA
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGTCGTCC AGGACTGGCC
    GTGATTGCAG GAGCCCTGGC CGTGGAATTG ATGGTATCTG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAT CGGATGAATG AGCCTCCAAC CTCTCTTGGG
    CTTGTGCCTC ACCAGATCCG GGGATTTCTT TCACGGTTTG ATAATGTCCT TCCCGTCAGC
    CTGGCATTTG ACAAATGTAC AGCTTGTTCT TCCAAAGTTC TTGATCAATA TGAACGAGAA
    GGATTTAACT TCCTAGCCAA GGTGTTTAAT TCTTCACATT CCTTCTTAGA AGACTTGACT
    GGTCTTACAT TGCTGCATCA AGAAACCCAA GCTGCTGAGC TTCATCAAGA GCCTCTGATG
    ACAAATATGC CTTTGGAGGC CAGCAGAAGC TGTGGGAAAC CCCCGTGTCA GCACTCAGAC
    CTGTTTCCAA TGGAGAGAAA TCGCTCTGGG GACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011531581.1
    CDS449..2644
    Translation

    Target ORF information:

    RefSeq Version XM_011533279.2
    Organism Homo sapiens(human)
    Definition Homo sapiens autophagy related 7 (ATG7), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011533279.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCGGCAG CTACGGGGGA TCCTGGACTC TCTAAACTGC AGTTTGCCCC TTTTAGTAGT 
    GCCTTGGATG TTGGGTTTTG GCATGAGTTG ACCCAGAAGA AGCTGAACGA GTATCGGCTG
    GATGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAATG GTGACTCTGC TGGGCTGCCA
    GCTCGCTTAA CATTGGAGTT CAGTGCTTTT GACATGAGTG CTCCCACCCC AGCCCGTTGC
    TGCCCAGCTA TTGGAACACT GTATAACACC AACACACTCG AGTCTTTCAA GACTGCAGAT
    AAGAAGCTCC TTTTGGAACA AGCAGCAAAT GAGATATGGG AATCCATAAA ATCAGGCACT
    GCTCTTGAAA ACCCTGTACT CCTCAACAAG TTCCTCCTCT TGACATTTGC AGATCTAAAG
    AAGTACCACT TCTACTATTG GTTTTGCTAT CCTGCCCTCT GTCTTCCAGA GAGTTTACCT
    CTCATTCAGG GGCCAGTGGG TTTGGATCAA AGGTTTTCAC TAAAACAGAT TGAAGCACTA
    GAGTGTGCAT ATGATAATCT TTGTCAAACA GAAGGAGTCA CAGCTCTTCC TTACTTCTTA
    ATCAAGTATG ATGAGAACAT GGTGCTGGTT TCCTTGCTTA AACACTACAG TGATTTCTTC
    CAAGGTCAAA GGACGAAGAT AACAATTGGT GTATATGATC CCTGTAACTT AGCCCAGTAC
    CCTGGATGGC CTTTGAGGAA TTTTTTGGTC CTAGCAGCCC ACAGATGGAG TAGCAGTTTC
    CAGTCTGTTG AAGTTGTTTG CTTCCGTGAC CGTACCATGC AGGGGGCGAG AGACGTTGCC
    CACAGCATCA TCTTCGAAGT GAAGCTTCCA GAAATGGCAT TTAGCCCAGA TTGTCCTAAA
    GCAGTTGGAT GGGAAAAGAA CCAGAAAGGA GGCATGGGAC CAAGGATGGT GAACCTCAGT
    GAATGTATGG ACCCTAAAAG GTTAGCTGAG TCATCAGTGG ATCTAAATCT CAAACTGATG
    TGTTGGAGAT TGGTTCCTAC TTTAGACTTG GACAAGGTTG TGTCTGTCAA ATGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGCAATGTA GCTAGGACGT TGATGGGTTG GGGCGTGAGA
    CACATCACAT TTGTGGACAA TGCCAAGATC TCCTACTCCA ATCCTGTGAG GCAGCCTCTC
    TATGAGTTTG AAGATTGCCT AGGGGGTGGT AAGCCCAAGG CTCTGGCAGC AGCGGACCGG
    CTCCAGAAAA TATTCCCCGG TGTGAATGCC AGAGGATTCA ACATGAGCAT ACCTATGCCT
    GGGCATCCAG TGAACTTCTC CAGTGTCACT CTGGAGCAAG CCCGCAGAGA TGTGGAGCAA
    CTGGAGCAGC TCATCGAAAG CCATGATGTC GTCTTCCTAT TGATGGACAC CAGGGAGAGC
    CGGTGGCTTC CTGCCGTCAT TGCTGCAAGC AAGAGAAAGC TGGTCATCAA TGCTGCTTTG
    GGATTTGACA CATTTGTTGT CATGAGACAT GGTCTGAAGA AACCAAAGCA GCAAGGAGCT
    GGGGACTTGT GTCCAAACCA CCCTGTGGCA TCTGCTGACC TCCTGGGCTC ATCGCTTTTT
    GCCAACATCC CTGGTTACAA GCTTGGCTGC TACTTCTGCA ATGATGTGGT GGCCCCAGGA
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGTCGTCC AGGACTGGCC
    GTGATTGCAG GAGCCCTGGC CGTGGAATTG ATGGTATCTG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAT CGGATGAATG AGCCTCCAAC CTCTCTTGGG
    CTTGTGCCTC ACCAGATCCG GGGATTTCTT TCACGGTTTG ATAATGTCCT TCCCGTCAGC
    CTGGCATTTG ACAAATGTAC AGCTTGTTCT TCCAAAGTTC TTGATCAATA TGAACGAGAA
    GGATTTAACT TCCTAGCCAA GGTGTTTAAT TCTTCACATT CCTTCTTAGA AGACTTGACT
    GGTCTTACAT TGCTGCATCA AGAAACCCAA GCTGCTGAGC TTCATCAAGA GCCTCTGATG
    ACAAATATGC CTTTGGAGGC CAGCAGAAGC TGTGGGAAAC CCCCGTGTCA GCACTCAGAC
    CTGTTTCCAA TGGAGAGAAA TCGCTCTGGG GACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu64106
    Clone ID Related Accession (Same CDS sequence) XM_011533279.2 , XM_024453312.1
    Accession Version XM_024453312.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2196bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product ubiquitin-like modifier-activating enzyme ATG7 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCGGCAG CTACGGGGGA TCCTGGACTC TCTAAACTGC AGTTTGCCCC TTTTAGTAGT 
    GCCTTGGATG TTGGGTTTTG GCATGAGTTG ACCCAGAAGA AGCTGAACGA GTATCGGCTG
    GATGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAATG GTGACTCTGC TGGGCTGCCA
    GCTCGCTTAA CATTGGAGTT CAGTGCTTTT GACATGAGTG CTCCCACCCC AGCCCGTTGC
    TGCCCAGCTA TTGGAACACT GTATAACACC AACACACTCG AGTCTTTCAA GACTGCAGAT
    AAGAAGCTCC TTTTGGAACA AGCAGCAAAT GAGATATGGG AATCCATAAA ATCAGGCACT
    GCTCTTGAAA ACCCTGTACT CCTCAACAAG TTCCTCCTCT TGACATTTGC AGATCTAAAG
    AAGTACCACT TCTACTATTG GTTTTGCTAT CCTGCCCTCT GTCTTCCAGA GAGTTTACCT
    CTCATTCAGG GGCCAGTGGG TTTGGATCAA AGGTTTTCAC TAAAACAGAT TGAAGCACTA
    GAGTGTGCAT ATGATAATCT TTGTCAAACA GAAGGAGTCA CAGCTCTTCC TTACTTCTTA
    ATCAAGTATG ATGAGAACAT GGTGCTGGTT TCCTTGCTTA AACACTACAG TGATTTCTTC
    CAAGGTCAAA GGACGAAGAT AACAATTGGT GTATATGATC CCTGTAACTT AGCCCAGTAC
    CCTGGATGGC CTTTGAGGAA TTTTTTGGTC CTAGCAGCCC ACAGATGGAG TAGCAGTTTC
    CAGTCTGTTG AAGTTGTTTG CTTCCGTGAC CGTACCATGC AGGGGGCGAG AGACGTTGCC
    CACAGCATCA TCTTCGAAGT GAAGCTTCCA GAAATGGCAT TTAGCCCAGA TTGTCCTAAA
    GCAGTTGGAT GGGAAAAGAA CCAGAAAGGA GGCATGGGAC CAAGGATGGT GAACCTCAGT
    GAATGTATGG ACCCTAAAAG GTTAGCTGAG TCATCAGTGG ATCTAAATCT CAAACTGATG
    TGTTGGAGAT TGGTTCCTAC TTTAGACTTG GACAAGGTTG TGTCTGTCAA ATGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGCAATGTA GCTAGGACGT TGATGGGTTG GGGCGTGAGA
    CACATCACAT TTGTGGACAA TGCCAAGATC TCCTACTCCA ATCCTGTGAG GCAGCCTCTC
    TATGAGTTTG AAGATTGCCT AGGGGGTGGT AAGCCCAAGG CTCTGGCAGC AGCGGACCGG
    CTCCAGAAAA TATTCCCCGG TGTGAATGCC AGAGGATTCA ACATGAGCAT ACCTATGCCT
    GGGCATCCAG TGAACTTCTC CAGTGTCACT CTGGAGCAAG CCCGCAGAGA TGTGGAGCAA
    CTGGAGCAGC TCATCGAAAG CCATGATGTC GTCTTCCTAT TGATGGACAC CAGGGAGAGC
    CGGTGGCTTC CTGCCGTCAT TGCTGCAAGC AAGAGAAAGC TGGTCATCAA TGCTGCTTTG
    GGATTTGACA CATTTGTTGT CATGAGACAT GGTCTGAAGA AACCAAAGCA GCAAGGAGCT
    GGGGACTTGT GTCCAAACCA CCCTGTGGCA TCTGCTGACC TCCTGGGCTC ATCGCTTTTT
    GCCAACATCC CTGGTTACAA GCTTGGCTGC TACTTCTGCA ATGATGTGGT GGCCCCAGGA
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGTCGTCC AGGACTGGCC
    GTGATTGCAG GAGCCCTGGC CGTGGAATTG ATGGTATCTG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAT CGGATGAATG AGCCTCCAAC CTCTCTTGGG
    CTTGTGCCTC ACCAGATCCG GGGATTTCTT TCACGGTTTG ATAATGTCCT TCCCGTCAGC
    CTGGCATTTG ACAAATGTAC AGCTTGTTCT TCCAAAGTTC TTGATCAATA TGAACGAGAA
    GGATTTAACT TCCTAGCCAA GGTGTTTAAT TCTTCACATT CCTTCTTAGA AGACTTGACT
    GGTCTTACAT TGCTGCATCA AGAAACCCAA GCTGCTGAGC TTCATCAAGA GCCTCTGATG
    ACAAATATGC CTTTGGAGGC CAGCAGAAGC TGTGGGAAAC CCCCGTGTCA GCACTCAGAC
    CTGTTTCCAA TGGAGAGAAA TCGCTCTGGG GACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024309080.1
    CDS61..2256
    Translation

    Target ORF information:

    RefSeq Version XM_024453312.1
    Organism Homo sapiens(human)
    Definition Homo sapiens autophagy related 7 (ATG7), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024453312.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCGGCAG CTACGGGGGA TCCTGGACTC TCTAAACTGC AGTTTGCCCC TTTTAGTAGT 
    GCCTTGGATG TTGGGTTTTG GCATGAGTTG ACCCAGAAGA AGCTGAACGA GTATCGGCTG
    GATGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAATG GTGACTCTGC TGGGCTGCCA
    GCTCGCTTAA CATTGGAGTT CAGTGCTTTT GACATGAGTG CTCCCACCCC AGCCCGTTGC
    TGCCCAGCTA TTGGAACACT GTATAACACC AACACACTCG AGTCTTTCAA GACTGCAGAT
    AAGAAGCTCC TTTTGGAACA AGCAGCAAAT GAGATATGGG AATCCATAAA ATCAGGCACT
    GCTCTTGAAA ACCCTGTACT CCTCAACAAG TTCCTCCTCT TGACATTTGC AGATCTAAAG
    AAGTACCACT TCTACTATTG GTTTTGCTAT CCTGCCCTCT GTCTTCCAGA GAGTTTACCT
    CTCATTCAGG GGCCAGTGGG TTTGGATCAA AGGTTTTCAC TAAAACAGAT TGAAGCACTA
    GAGTGTGCAT ATGATAATCT TTGTCAAACA GAAGGAGTCA CAGCTCTTCC TTACTTCTTA
    ATCAAGTATG ATGAGAACAT GGTGCTGGTT TCCTTGCTTA AACACTACAG TGATTTCTTC
    CAAGGTCAAA GGACGAAGAT AACAATTGGT GTATATGATC CCTGTAACTT AGCCCAGTAC
    CCTGGATGGC CTTTGAGGAA TTTTTTGGTC CTAGCAGCCC ACAGATGGAG TAGCAGTTTC
    CAGTCTGTTG AAGTTGTTTG CTTCCGTGAC CGTACCATGC AGGGGGCGAG AGACGTTGCC
    CACAGCATCA TCTTCGAAGT GAAGCTTCCA GAAATGGCAT TTAGCCCAGA TTGTCCTAAA
    GCAGTTGGAT GGGAAAAGAA CCAGAAAGGA GGCATGGGAC CAAGGATGGT GAACCTCAGT
    GAATGTATGG ACCCTAAAAG GTTAGCTGAG TCATCAGTGG ATCTAAATCT CAAACTGATG
    TGTTGGAGAT TGGTTCCTAC TTTAGACTTG GACAAGGTTG TGTCTGTCAA ATGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGCAATGTA GCTAGGACGT TGATGGGTTG GGGCGTGAGA
    CACATCACAT TTGTGGACAA TGCCAAGATC TCCTACTCCA ATCCTGTGAG GCAGCCTCTC
    TATGAGTTTG AAGATTGCCT AGGGGGTGGT AAGCCCAAGG CTCTGGCAGC AGCGGACCGG
    CTCCAGAAAA TATTCCCCGG TGTGAATGCC AGAGGATTCA ACATGAGCAT ACCTATGCCT
    GGGCATCCAG TGAACTTCTC CAGTGTCACT CTGGAGCAAG CCCGCAGAGA TGTGGAGCAA
    CTGGAGCAGC TCATCGAAAG CCATGATGTC GTCTTCCTAT TGATGGACAC CAGGGAGAGC
    CGGTGGCTTC CTGCCGTCAT TGCTGCAAGC AAGAGAAAGC TGGTCATCAA TGCTGCTTTG
    GGATTTGACA CATTTGTTGT CATGAGACAT GGTCTGAAGA AACCAAAGCA GCAAGGAGCT
    GGGGACTTGT GTCCAAACCA CCCTGTGGCA TCTGCTGACC TCCTGGGCTC ATCGCTTTTT
    GCCAACATCC CTGGTTACAA GCTTGGCTGC TACTTCTGCA ATGATGTGGT GGCCCCAGGA
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGTCGTCC AGGACTGGCC
    GTGATTGCAG GAGCCCTGGC CGTGGAATTG ATGGTATCTG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAT CGGATGAATG AGCCTCCAAC CTCTCTTGGG
    CTTGTGCCTC ACCAGATCCG GGGATTTCTT TCACGGTTTG ATAATGTCCT TCCCGTCAGC
    CTGGCATTTG ACAAATGTAC AGCTTGTTCT TCCAAAGTTC TTGATCAATA TGAACGAGAA
    GGATTTAACT TCCTAGCCAA GGTGTTTAAT TCTTCACATT CCTTCTTAGA AGACTTGACT
    GGTCTTACAT TGCTGCATCA AGAAACCCAA GCTGCTGAGC TTCATCAAGA GCCTCTGATG
    ACAAATATGC CTTTGGAGGC CAGCAGAAGC TGTGGGAAAC CCCCGTGTCA GCACTCAGAC
    CTGTTTCCAA TGGAGAGAAA TCGCTCTGGG GACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu64107
    Clone ID Related Accession (Same CDS sequence) XM_011533280.2 , XM_017005543.1
    Accession Version XM_011533280.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2136bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product ubiquitin-like modifier-activating enzyme ATG7 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011533280.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGCGGCAG CTACGGGGGA TCCTGGACTC TCTAAACTGC AGTTTGCCCC TTTTAGTAGT 
    GCCTTGGATG TTGGGTTTTG GCATGAGTTG ACCCAGAAGA AGCTGAACGA GTATCGGCTG
    GATGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAATG GTGACTCTGC TGGGCTGCCA
    GCTCGCTTAA CATTGGAGTT CAGTGCTTTT GACATGAGTG CTCCCACCCC AGCCCGTTGC
    TGCCCAGCTA TTGGAACACT GTATAACACC AACACACTCG AGTCTTTCAA GACTGCAGAT
    AAGAAGCTCC TTTTGGAACA AGCAGCAAAT GAGATATGGG AATCCATAAA ATCAGGCACT
    GCTCTTGAAA ACCCTGTACT CCTCAACAAG TTCCTCCTCT TGACATTTGC AGATCTAAAG
    AAGTACCACT TCTACTATTG GTTTTGCTAT CCTGCCCTCT GTCTTCCAGA GAGTTTACCT
    CTCATTCAGG GGCCAGTGGG TTTGGATCAA AGGTTTTCAC TAAAACAGAT TGAAGCACTA
    GAGTGTGCAT ATGATAATCT TTGTCAAACA GAAGGAGTCA CAGCTCTTCC TTACTTCTTA
    ATCAAGTATG ATGAGAACAT GGTGCTGGTT TCCTTGCTTA AACACTACAG TGATTTCTTC
    CAAGGTCAAA GGACGAAGAT AACAATTGGT GTATATGATC CCTGTAACTT AGCCCAGTAC
    CCTGGATGGC CTTTGAGGAA TTTTTTGGTC CTAGCAGCCC ACAGATGGAG TAGCAGTTTC
    CAGTCTGTTG AAGTTGTTTG CTTCCGTGAC CGTACCATGC AGGGGGCGAG AGACGTTGCC
    CACAGCATCA TCTTCGAAGT GAAGCTTCCA GAAATGGCAT TTAGCCCAGA TTGTCCTAAA
    GCAGTTGGAT GGGAAAAGAA CCAGAAAGGA GGCATGGGAC CAAGGATGGT GAACCTCAGT
    GAATGTATGG ACCCTAAAAG GTTAGCTGAG TCATCAGTGG ATCTAAATCT CAAACTGATG
    TGTTGGAGAT TGGTTCCTAC TTTAGACTTG GACAAGGTTG TGTCTGTCAA ATGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGCAATGTA GCTAGGACGT TGATGGGTTG GGGCGTGAGA
    CACATCACAT TTGTGGACAA TGCCAAGATC TCCTACTCCA ATCCTGTGAG GCAGCCTCTC
    TATGAGTTTG AAGATTGCCT AGGGGGTGGT AAGCCCAAGG CTCTGGCAGC AGCGGACCGG
    CTCCAGAAAA TATTCCCCGG TGTGAATGCC AGAGGATTCA ACATGAGCAT ACCTATGCCT
    GGGCATCCAG TGAACTTCTC CAGTGTCACT CTGGAGCAAG CCCGCAGAGA TGTGGAGCAA
    CTGGAGCAGC TCATCGAAAG CCATGATGTC GTCTTCCTAT TGATGGACAC CAGGGAGAGC
    CGGTGGCTTC CTGCCGTCAT TGCTGCAAGC AAGAGAAAGC TGGTCATCAA TGCTGCTTTG
    GGATTTGACA CATTTGTTGT CATGAGACAT GGTCTGAAGA AACCAAAGCA GCAAGGAGCT
    GGGGACTTGT GTCCAAACCA CCCTGTGGCA TCTGCTGACC TCCTGGGCTC ATCGCTTTTT
    GCCAACATCC CTGGTTACAA GCTTGGCTGC TACTTCTGCA ATGATGTGGT GGCCCCAGGA
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGTCGTCC AGGACTGGCC
    GTGATTGCAG GAGCCCTGGC CGTGGAATTG ATGGTATCTG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAT CGGATGAATG AGCCTCCAAC CTCTCTTGGG
    CTTGTGCCTC ACCAGGTTCT TGATCAATAT GAACGAGAAG GATTTAACTT CCTAGCCAAG
    GTGTTTAATT CTTCACATTC CTTCTTAGAA GACTTGACTG GTCTTACATT GCTGCATCAA
    GAAACCCAAG CTGCTGAGGA TCATCTTTCT TTCATCCGGC CCAAGAATGG CACCTTCCCC
    GCCCATCAGG CCACCTGCAC TCAGGAGTCG GCTGCCTGTC CTGAAATAGA TCTCTACCAG
    CTCTCAAGCA GAACAATTCC TTGCATTCAT ACTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011531582.1
    CDS449..2584
    Translation

    Target ORF information:

    RefSeq Version XM_011533280.2
    Organism Homo sapiens(human)
    Definition Homo sapiens autophagy related 7 (ATG7), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011533280.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGCGGCAG CTACGGGGGA TCCTGGACTC TCTAAACTGC AGTTTGCCCC TTTTAGTAGT 
    GCCTTGGATG TTGGGTTTTG GCATGAGTTG ACCCAGAAGA AGCTGAACGA GTATCGGCTG
    GATGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAATG GTGACTCTGC TGGGCTGCCA
    GCTCGCTTAA CATTGGAGTT CAGTGCTTTT GACATGAGTG CTCCCACCCC AGCCCGTTGC
    TGCCCAGCTA TTGGAACACT GTATAACACC AACACACTCG AGTCTTTCAA GACTGCAGAT
    AAGAAGCTCC TTTTGGAACA AGCAGCAAAT GAGATATGGG AATCCATAAA ATCAGGCACT
    GCTCTTGAAA ACCCTGTACT CCTCAACAAG TTCCTCCTCT TGACATTTGC AGATCTAAAG
    AAGTACCACT TCTACTATTG GTTTTGCTAT CCTGCCCTCT GTCTTCCAGA GAGTTTACCT
    CTCATTCAGG GGCCAGTGGG TTTGGATCAA AGGTTTTCAC TAAAACAGAT TGAAGCACTA
    GAGTGTGCAT ATGATAATCT TTGTCAAACA GAAGGAGTCA CAGCTCTTCC TTACTTCTTA
    ATCAAGTATG ATGAGAACAT GGTGCTGGTT TCCTTGCTTA AACACTACAG TGATTTCTTC
    CAAGGTCAAA GGACGAAGAT AACAATTGGT GTATATGATC CCTGTAACTT AGCCCAGTAC
    CCTGGATGGC CTTTGAGGAA TTTTTTGGTC CTAGCAGCCC ACAGATGGAG TAGCAGTTTC
    CAGTCTGTTG AAGTTGTTTG CTTCCGTGAC CGTACCATGC AGGGGGCGAG AGACGTTGCC
    CACAGCATCA TCTTCGAAGT GAAGCTTCCA GAAATGGCAT TTAGCCCAGA TTGTCCTAAA
    GCAGTTGGAT GGGAAAAGAA CCAGAAAGGA GGCATGGGAC CAAGGATGGT GAACCTCAGT
    GAATGTATGG ACCCTAAAAG GTTAGCTGAG TCATCAGTGG ATCTAAATCT CAAACTGATG
    TGTTGGAGAT TGGTTCCTAC TTTAGACTTG GACAAGGTTG TGTCTGTCAA ATGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGCAATGTA GCTAGGACGT TGATGGGTTG GGGCGTGAGA
    CACATCACAT TTGTGGACAA TGCCAAGATC TCCTACTCCA ATCCTGTGAG GCAGCCTCTC
    TATGAGTTTG AAGATTGCCT AGGGGGTGGT AAGCCCAAGG CTCTGGCAGC AGCGGACCGG
    CTCCAGAAAA TATTCCCCGG TGTGAATGCC AGAGGATTCA ACATGAGCAT ACCTATGCCT
    GGGCATCCAG TGAACTTCTC CAGTGTCACT CTGGAGCAAG CCCGCAGAGA TGTGGAGCAA
    CTGGAGCAGC TCATCGAAAG CCATGATGTC GTCTTCCTAT TGATGGACAC CAGGGAGAGC
    CGGTGGCTTC CTGCCGTCAT TGCTGCAAGC AAGAGAAAGC TGGTCATCAA TGCTGCTTTG
    GGATTTGACA CATTTGTTGT CATGAGACAT GGTCTGAAGA AACCAAAGCA GCAAGGAGCT
    GGGGACTTGT GTCCAAACCA CCCTGTGGCA TCTGCTGACC TCCTGGGCTC ATCGCTTTTT
    GCCAACATCC CTGGTTACAA GCTTGGCTGC TACTTCTGCA ATGATGTGGT GGCCCCAGGA
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGTCGTCC AGGACTGGCC
    GTGATTGCAG GAGCCCTGGC CGTGGAATTG ATGGTATCTG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAT CGGATGAATG AGCCTCCAAC CTCTCTTGGG
    CTTGTGCCTC ACCAGGTTCT TGATCAATAT GAACGAGAAG GATTTAACTT CCTAGCCAAG
    GTGTTTAATT CTTCACATTC CTTCTTAGAA GACTTGACTG GTCTTACATT GCTGCATCAA
    GAAACCCAAG CTGCTGAGGA TCATCTTTCT TTCATCCGGC CCAAGAATGG CACCTTCCCC
    GCCCATCAGG CCACCTGCAC TCAGGAGTCG GCTGCCTGTC CTGAAATAGA TCTCTACCAG
    CTCTCAAGCA GAACAATTCC TTGCATTCAT ACTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu64107
    Clone ID Related Accession (Same CDS sequence) XM_011533280.2 , XM_017005543.1
    Accession Version XM_017005543.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2136bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product ubiquitin-like modifier-activating enzyme ATG7 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGCGGCAG CTACGGGGGA TCCTGGACTC TCTAAACTGC AGTTTGCCCC TTTTAGTAGT 
    GCCTTGGATG TTGGGTTTTG GCATGAGTTG ACCCAGAAGA AGCTGAACGA GTATCGGCTG
    GATGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAATG GTGACTCTGC TGGGCTGCCA
    GCTCGCTTAA CATTGGAGTT CAGTGCTTTT GACATGAGTG CTCCCACCCC AGCCCGTTGC
    TGCCCAGCTA TTGGAACACT GTATAACACC AACACACTCG AGTCTTTCAA GACTGCAGAT
    AAGAAGCTCC TTTTGGAACA AGCAGCAAAT GAGATATGGG AATCCATAAA ATCAGGCACT
    GCTCTTGAAA ACCCTGTACT CCTCAACAAG TTCCTCCTCT TGACATTTGC AGATCTAAAG
    AAGTACCACT TCTACTATTG GTTTTGCTAT CCTGCCCTCT GTCTTCCAGA GAGTTTACCT
    CTCATTCAGG GGCCAGTGGG TTTGGATCAA AGGTTTTCAC TAAAACAGAT TGAAGCACTA
    GAGTGTGCAT ATGATAATCT TTGTCAAACA GAAGGAGTCA CAGCTCTTCC TTACTTCTTA
    ATCAAGTATG ATGAGAACAT GGTGCTGGTT TCCTTGCTTA AACACTACAG TGATTTCTTC
    CAAGGTCAAA GGACGAAGAT AACAATTGGT GTATATGATC CCTGTAACTT AGCCCAGTAC
    CCTGGATGGC CTTTGAGGAA TTTTTTGGTC CTAGCAGCCC ACAGATGGAG TAGCAGTTTC
    CAGTCTGTTG AAGTTGTTTG CTTCCGTGAC CGTACCATGC AGGGGGCGAG AGACGTTGCC
    CACAGCATCA TCTTCGAAGT GAAGCTTCCA GAAATGGCAT TTAGCCCAGA TTGTCCTAAA
    GCAGTTGGAT GGGAAAAGAA CCAGAAAGGA GGCATGGGAC CAAGGATGGT GAACCTCAGT
    GAATGTATGG ACCCTAAAAG GTTAGCTGAG TCATCAGTGG ATCTAAATCT CAAACTGATG
    TGTTGGAGAT TGGTTCCTAC TTTAGACTTG GACAAGGTTG TGTCTGTCAA ATGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGCAATGTA GCTAGGACGT TGATGGGTTG GGGCGTGAGA
    CACATCACAT TTGTGGACAA TGCCAAGATC TCCTACTCCA ATCCTGTGAG GCAGCCTCTC
    TATGAGTTTG AAGATTGCCT AGGGGGTGGT AAGCCCAAGG CTCTGGCAGC AGCGGACCGG
    CTCCAGAAAA TATTCCCCGG TGTGAATGCC AGAGGATTCA ACATGAGCAT ACCTATGCCT
    GGGCATCCAG TGAACTTCTC CAGTGTCACT CTGGAGCAAG CCCGCAGAGA TGTGGAGCAA
    CTGGAGCAGC TCATCGAAAG CCATGATGTC GTCTTCCTAT TGATGGACAC CAGGGAGAGC
    CGGTGGCTTC CTGCCGTCAT TGCTGCAAGC AAGAGAAAGC TGGTCATCAA TGCTGCTTTG
    GGATTTGACA CATTTGTTGT CATGAGACAT GGTCTGAAGA AACCAAAGCA GCAAGGAGCT
    GGGGACTTGT GTCCAAACCA CCCTGTGGCA TCTGCTGACC TCCTGGGCTC ATCGCTTTTT
    GCCAACATCC CTGGTTACAA GCTTGGCTGC TACTTCTGCA ATGATGTGGT GGCCCCAGGA
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGTCGTCC AGGACTGGCC
    GTGATTGCAG GAGCCCTGGC CGTGGAATTG ATGGTATCTG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAT CGGATGAATG AGCCTCCAAC CTCTCTTGGG
    CTTGTGCCTC ACCAGGTTCT TGATCAATAT GAACGAGAAG GATTTAACTT CCTAGCCAAG
    GTGTTTAATT CTTCACATTC CTTCTTAGAA GACTTGACTG GTCTTACATT GCTGCATCAA
    GAAACCCAAG CTGCTGAGGA TCATCTTTCT TTCATCCGGC CCAAGAATGG CACCTTCCCC
    GCCCATCAGG CCACCTGCAC TCAGGAGTCG GCTGCCTGTC CTGAAATAGA TCTCTACCAG
    CTCTCAAGCA GAACAATTCC TTGCATTCAT ACTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016861032.1
    CDS61..2196
    Translation

    Target ORF information:

    RefSeq Version XM_017005543.1
    Organism Homo sapiens(human)
    Definition Homo sapiens autophagy related 7 (ATG7), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017005543.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGCGGCAG CTACGGGGGA TCCTGGACTC TCTAAACTGC AGTTTGCCCC TTTTAGTAGT 
    GCCTTGGATG TTGGGTTTTG GCATGAGTTG ACCCAGAAGA AGCTGAACGA GTATCGGCTG
    GATGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAATG GTGACTCTGC TGGGCTGCCA
    GCTCGCTTAA CATTGGAGTT CAGTGCTTTT GACATGAGTG CTCCCACCCC AGCCCGTTGC
    TGCCCAGCTA TTGGAACACT GTATAACACC AACACACTCG AGTCTTTCAA GACTGCAGAT
    AAGAAGCTCC TTTTGGAACA AGCAGCAAAT GAGATATGGG AATCCATAAA ATCAGGCACT
    GCTCTTGAAA ACCCTGTACT CCTCAACAAG TTCCTCCTCT TGACATTTGC AGATCTAAAG
    AAGTACCACT TCTACTATTG GTTTTGCTAT CCTGCCCTCT GTCTTCCAGA GAGTTTACCT
    CTCATTCAGG GGCCAGTGGG TTTGGATCAA AGGTTTTCAC TAAAACAGAT TGAAGCACTA
    GAGTGTGCAT ATGATAATCT TTGTCAAACA GAAGGAGTCA CAGCTCTTCC TTACTTCTTA
    ATCAAGTATG ATGAGAACAT GGTGCTGGTT TCCTTGCTTA AACACTACAG TGATTTCTTC
    CAAGGTCAAA GGACGAAGAT AACAATTGGT GTATATGATC CCTGTAACTT AGCCCAGTAC
    CCTGGATGGC CTTTGAGGAA TTTTTTGGTC CTAGCAGCCC ACAGATGGAG TAGCAGTTTC
    CAGTCTGTTG AAGTTGTTTG CTTCCGTGAC CGTACCATGC AGGGGGCGAG AGACGTTGCC
    CACAGCATCA TCTTCGAAGT GAAGCTTCCA GAAATGGCAT TTAGCCCAGA TTGTCCTAAA
    GCAGTTGGAT GGGAAAAGAA CCAGAAAGGA GGCATGGGAC CAAGGATGGT GAACCTCAGT
    GAATGTATGG ACCCTAAAAG GTTAGCTGAG TCATCAGTGG ATCTAAATCT CAAACTGATG
    TGTTGGAGAT TGGTTCCTAC TTTAGACTTG GACAAGGTTG TGTCTGTCAA ATGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGCAATGTA GCTAGGACGT TGATGGGTTG GGGCGTGAGA
    CACATCACAT TTGTGGACAA TGCCAAGATC TCCTACTCCA ATCCTGTGAG GCAGCCTCTC
    TATGAGTTTG AAGATTGCCT AGGGGGTGGT AAGCCCAAGG CTCTGGCAGC AGCGGACCGG
    CTCCAGAAAA TATTCCCCGG TGTGAATGCC AGAGGATTCA ACATGAGCAT ACCTATGCCT
    GGGCATCCAG TGAACTTCTC CAGTGTCACT CTGGAGCAAG CCCGCAGAGA TGTGGAGCAA
    CTGGAGCAGC TCATCGAAAG CCATGATGTC GTCTTCCTAT TGATGGACAC CAGGGAGAGC
    CGGTGGCTTC CTGCCGTCAT TGCTGCAAGC AAGAGAAAGC TGGTCATCAA TGCTGCTTTG
    GGATTTGACA CATTTGTTGT CATGAGACAT GGTCTGAAGA AACCAAAGCA GCAAGGAGCT
    GGGGACTTGT GTCCAAACCA CCCTGTGGCA TCTGCTGACC TCCTGGGCTC ATCGCTTTTT
    GCCAACATCC CTGGTTACAA GCTTGGCTGC TACTTCTGCA ATGATGTGGT GGCCCCAGGA
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGTCGTCC AGGACTGGCC
    GTGATTGCAG GAGCCCTGGC CGTGGAATTG ATGGTATCTG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAT CGGATGAATG AGCCTCCAAC CTCTCTTGGG
    CTTGTGCCTC ACCAGGTTCT TGATCAATAT GAACGAGAAG GATTTAACTT CCTAGCCAAG
    GTGTTTAATT CTTCACATTC CTTCTTAGAA GACTTGACTG GTCTTACATT GCTGCATCAA
    GAAACCCAAG CTGCTGAGGA TCATCTTTCT TTCATCCGGC CCAAGAATGG CACCTTCCCC
    GCCCATCAGG CCACCTGCAC TCAGGAGTCG GCTGCCTGTC CTGAAATAGA TCTCTACCAG
    CTCTCAAGCA GAACAATTCC TTGCATTCAT ACTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu64108
    Clone ID Related Accession (Same CDS sequence) XM_011533281.2
    Accession Version XM_011533281.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2133bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product ubiquitin-like modifier-activating enzyme ATG7 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011533281.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGCGGCAG CTACGGGGGA TCCTGGACTC TCTAAACTGC AGTTTGCCCC TTTTAGTAGT 
    GCCTTGGATG TTGGGTTTTG GCATGAGTTG ACCCAGAAGA AGCTGAACGA GTATCGGCTG
    GATGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAATG GTGACTCTGC TGGGCTGCCA
    GCTCGCTTAA CATTGGAGTT CAGTGCTTTT GACATGAGTG CTCCCACCCC AGCCCGTTGC
    TGCCCAGCTA TTGGAACACT GTATAACACC AACACACTCG AGTCTTTCAA GACTGCAGAT
    AAGAAGCTCC TTTTGGAACA AGCAGCAAAT GAGATATGGG AATCCATAAA ATCAGGCACT
    GCTCTTGAAA ACCCTGTACT CCTCAACAAG TTCCTCCTCT TGACATTTGC AGATCTAAAG
    AAGTACCACT TCTACTATTG GTTTTGCTAT CCTGCCCTCT GTCTTCCAGA GAGTTTACCT
    CTCATTCAGG GGCCAGTGGG TTTGGATCAA AGGTTTTCAC TAAAACAGAT TGAAGCACTA
    GAGTGTGCAT ATGATAATCT TTGTCAAACA GAAGGAGTCA CAGCTCTTCC TTACTTCTTA
    ATCAAGTATG ATGAGAACAT GGTGCTGGTT TCCTTGCTTA AACACTACAG TGATTTCTTC
    CAAGGTCAAA GGACGAAGAT AACAATTGGT GTATATGATC CCTGTAACTT AGCCCAGTAC
    CCTGGATGGC CTTTGAGGAA TTTTTTGGTC CTAGCAGCCC ACAGATGGAG TAGCAGTTTC
    CAGTCTGTTG AAGTTGTTTG CTTCCGTGAC CGTACCATGC AGGGGGCGAG AGACGTTGCC
    CACAGCATCA TCTTCGAAGT GAAGCTTCCA GAAATGGCAT TTAGCCCAGA TTGTCCTAAA
    GCAGTTGGAT GGGAAAAGAA CCAGAAAGGA GGCATGGGAC CAAGGATGGT GAACCTCAGT
    GAATGTATGG ACCCTAAAAG GTTAGCTGAG TCATCAGTGG ATCTAAATCT CAAACTGATG
    TGTTGGAGAT TGGTTCCTAC TTTAGACTTG GACAAGGTTG TGTCTGTCAA ATGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGCAATGTA GCTAGGACGT TGATGGGTTG GGGCGTGAGA
    CACATCACAT TTGTGGACAA TGCCAAGATC TCCTACTCCA ATCCTGTGAG GCAGCCTCTC
    TATGAGTTTG AAGATTGCCT AGGGGGTGGT AAGCCCAAGG CTCTGGCAGC AGCGGACCGG
    CTCCAGAAAA TATTCCCCGG TGTGAATGCC AGAGGATTCA ACATGAGCAT ACCTATGCCT
    GGGCATCCAG TGAACTTCTC CAGTGTCACT CTGGAGCAAG CCCGCAGAGA TGTGGAGCAA
    CTGGAGCAGC TCATCGAAAG CCATGATGTC GTCTTCCTAT TGATGGACAC CAGGGAGAGC
    CGGTGGCTTC CTGCCGTCAT TGCTGCAAGC AAGAGAAAGC TGGTCATCAA TGCTGCTTTG
    GGATTTGACA CATTTGTTGT CATGAGACAT GGTCTGAAGA AACCAAAGCA GCAAGGAGCT
    GGGGACTTGT GTCCAAACCA CCCTGTGGCA TCTGCTGACC TCCTGGGCTC ATCGCTTTTT
    GCCAACATCC CTGGTTACAA GCTTGGCTGC TACTTCTGCA ATGATGTGGT GGCCCCAGGA
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGTCGTCC AGGACTGGCC
    GTGATTGCAG GAGCCCTGGC CGTGGAATTG ATGGTATCTG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAT CGGATGAATG AGCCTCCAAC CTCTCTTGGG
    CTTGTGCCTC ACCAGATCCG GGGATTTCTT TCACGGTTTG ATAATGTCCT TCCCGTCAGC
    CTGGCATTTG ACAAATGTAC AGCTTGTTCT TCCAAAGTTC TTGATCAATA TGAACGAGAA
    GGATTTAACT TCCTAGCCAA GGTGTTTAAT TCTTCACATT CCTTCTTAGA AGACTTGACT
    GGTCTTACAT TGCTGCATCA AGAAACCCAA GCTGCTGAGA TGGAGAAATT AAGGCACATG
    GAACCTAAGT GTTCAAGGTC ATGGAGCCAG TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011531583.1
    CDS449..2581
    Translation

    Target ORF information:

    RefSeq Version XM_011533281.2
    Organism Homo sapiens(human)
    Definition Homo sapiens autophagy related 7 (ATG7), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011533281.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGCGGCAG CTACGGGGGA TCCTGGACTC TCTAAACTGC AGTTTGCCCC TTTTAGTAGT 
    GCCTTGGATG TTGGGTTTTG GCATGAGTTG ACCCAGAAGA AGCTGAACGA GTATCGGCTG
    GATGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAATG GTGACTCTGC TGGGCTGCCA
    GCTCGCTTAA CATTGGAGTT CAGTGCTTTT GACATGAGTG CTCCCACCCC AGCCCGTTGC
    TGCCCAGCTA TTGGAACACT GTATAACACC AACACACTCG AGTCTTTCAA GACTGCAGAT
    AAGAAGCTCC TTTTGGAACA AGCAGCAAAT GAGATATGGG AATCCATAAA ATCAGGCACT
    GCTCTTGAAA ACCCTGTACT CCTCAACAAG TTCCTCCTCT TGACATTTGC AGATCTAAAG
    AAGTACCACT TCTACTATTG GTTTTGCTAT CCTGCCCTCT GTCTTCCAGA GAGTTTACCT
    CTCATTCAGG GGCCAGTGGG TTTGGATCAA AGGTTTTCAC TAAAACAGAT TGAAGCACTA
    GAGTGTGCAT ATGATAATCT TTGTCAAACA GAAGGAGTCA CAGCTCTTCC TTACTTCTTA
    ATCAAGTATG ATGAGAACAT GGTGCTGGTT TCCTTGCTTA AACACTACAG TGATTTCTTC
    CAAGGTCAAA GGACGAAGAT AACAATTGGT GTATATGATC CCTGTAACTT AGCCCAGTAC
    CCTGGATGGC CTTTGAGGAA TTTTTTGGTC CTAGCAGCCC ACAGATGGAG TAGCAGTTTC
    CAGTCTGTTG AAGTTGTTTG CTTCCGTGAC CGTACCATGC AGGGGGCGAG AGACGTTGCC
    CACAGCATCA TCTTCGAAGT GAAGCTTCCA GAAATGGCAT TTAGCCCAGA TTGTCCTAAA
    GCAGTTGGAT GGGAAAAGAA CCAGAAAGGA GGCATGGGAC CAAGGATGGT GAACCTCAGT
    GAATGTATGG ACCCTAAAAG GTTAGCTGAG TCATCAGTGG ATCTAAATCT CAAACTGATG
    TGTTGGAGAT TGGTTCCTAC TTTAGACTTG GACAAGGTTG TGTCTGTCAA ATGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGCAATGTA GCTAGGACGT TGATGGGTTG GGGCGTGAGA
    CACATCACAT TTGTGGACAA TGCCAAGATC TCCTACTCCA ATCCTGTGAG GCAGCCTCTC
    TATGAGTTTG AAGATTGCCT AGGGGGTGGT AAGCCCAAGG CTCTGGCAGC AGCGGACCGG
    CTCCAGAAAA TATTCCCCGG TGTGAATGCC AGAGGATTCA ACATGAGCAT ACCTATGCCT
    GGGCATCCAG TGAACTTCTC CAGTGTCACT CTGGAGCAAG CCCGCAGAGA TGTGGAGCAA
    CTGGAGCAGC TCATCGAAAG CCATGATGTC GTCTTCCTAT TGATGGACAC CAGGGAGAGC
    CGGTGGCTTC CTGCCGTCAT TGCTGCAAGC AAGAGAAAGC TGGTCATCAA TGCTGCTTTG
    GGATTTGACA CATTTGTTGT CATGAGACAT GGTCTGAAGA AACCAAAGCA GCAAGGAGCT
    GGGGACTTGT GTCCAAACCA CCCTGTGGCA TCTGCTGACC TCCTGGGCTC ATCGCTTTTT
    GCCAACATCC CTGGTTACAA GCTTGGCTGC TACTTCTGCA ATGATGTGGT GGCCCCAGGA
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGTCGTCC AGGACTGGCC
    GTGATTGCAG GAGCCCTGGC CGTGGAATTG ATGGTATCTG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAT CGGATGAATG AGCCTCCAAC CTCTCTTGGG
    CTTGTGCCTC ACCAGATCCG GGGATTTCTT TCACGGTTTG ATAATGTCCT TCCCGTCAGC
    CTGGCATTTG ACAAATGTAC AGCTTGTTCT TCCAAAGTTC TTGATCAATA TGAACGAGAA
    GGATTTAACT TCCTAGCCAA GGTGTTTAAT TCTTCACATT CCTTCTTAGA AGACTTGACT
    GGTCTTACAT TGCTGCATCA AGAAACCCAA GCTGCTGAGA TGGAGAAATT AAGGCACATG
    GAACCTAAGT GTTCAAGGTC ATGGAGCCAG TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu64109
    Clone ID Related Accession (Same CDS sequence) XM_011533284.2
    Accession Version XM_011533284.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2085bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product ubiquitin-like modifier-activating enzyme ATG7 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011533284.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGCGGCAG CTACGGGGGA TCCTGGACTC TCTAAACTGC AGTTTGCCCC TTTTAGTAGT 
    GCCTTGGATG TTGGGTTTTG GCATGAGTTG ACCCAGAAGA AGCTGAACGA GTATCGGCTG
    GATGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAATG GTGACTCTGC TGGGCTGCCA
    GCTCGCTTAA CATTGGAGTT CAGTGCTTTT GACATGAGTG CTCCCACCCC AGCCCGTTGC
    TGCCCAGCTA TTGGAACACT GTATAACACC AACACACTCG AGTCTTTCAA GACTGCAGAT
    AAGAAGCTCC TTTTGGAACA AGCAGCAAAT GAGATATGGG AATCCATAAA ATCAGGCACT
    GCTCTTGAAA ACCCTGTACT CCTCAACAAG TTCCTCCTCT TGACATTTGC AGATCTAAAG
    AAGTACCACT TCTACTATTG GTTTTGCTAT CCTGCCCTCT GTCTTCCAGA GAGTTTACCT
    CTCATTCAGG GGCCAGTGGG TTTGGATCAA AGGTTTTCAC TAAAACAGAT TGAAGCACTA
    GAGTGTGCAT ATGATAATCT TTGTCAAACA GAAGGAGTCA CAGCTCTTCC TTACTTCTTA
    ATCAAGTATG ATGAGAACAT GGTGCTGGTT TCCTTGCTTA AACACTACAG TGATTTCTTC
    CAAGGTCAAA GGACGAAGAT AACAATTGGT GTATATGATC CCTGTAACTT AGCCCAGTAC
    CCTGGATGGC CTTTGAGGAA TTTTTTGGTC CTAGCAGCCC ACAGATGGAG TAGCAGTTTC
    CAGTCTGTTG AAGTTGTTTG CTTCCGTGAC CGTACCATGC AGGGGGCGAG AGACGTTGCC
    CACAGCATCA TCTTCGAAGT GAAGCTTCCA GAAATGGCAT TTAGCCCAGA TTGTCCTAAA
    GCAGTTGGAT GGGAAAAGAA CCAGAAAGGA GGCATGGGAC CAAGGATGGT GAACCTCAGT
    GAATGTATGG ACCCTAAAAG GTTAGCTGAG TCATCAGTGG ATCTAAATCT CAAACTGATG
    TGTTGGAGAT TGGTTCCTAC TTTAGACTTG GACAAGGTTG TGTCTGTCAA ATGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGCAATGTA GCTAGGACGT TGATGGGTTG GGGCGTGAGA
    CACATCACAT TTGTGGACAA TGCCAAGATC TCCTACTCCA ATCCTGTGAG GCAGCCTCTC
    TATGAGTTTG AAGATTGCCT AGGGGGTGGT AAGCCCAAGG CTCTGGCAGC AGCGGACCGG
    CTCCAGAAAA TATTCCCCGG TGTGAATGCC AGAGGATTCA ACATGAGCAT ACCTATGCCT
    GGGCATCCAG TGAACTTCTC CAGTGTCACT CTGGAGCAAG CCCGCAGAGA TGTGGAGCAA
    CTGGAGCAGC TCATCGAAAG CCATGATGTC GTCTTCCTAT TGATGGACAC CAGGGAGAGC
    CGGTGGCTTC CTGCCGTCAT TGCTGCAAGC AAGAGAAAGC TGGTCATCAA TGCTGCTTTG
    GGATTTGACA CATTTGTTGT CATGAGACAT GGTCTGAAGA AACCAAAGCA GCAAGGAGCT
    GGGGACTTGT GTCCAAACCA CCCTGTGGCA TCTGCTGACC TCCTGGGCTC ATCGCTTTTT
    GCCAACATCC CTGGTTACAA GCTTGGCTGC TACTTCTGCA ATGATGTGGT GGCCCCAGGA
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGTCGTCC AGGACTGGCC
    GTGATTGCAG GAGCCCTGGC CGTGGAATTG ATGGTATCTG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAT CGGATGAATG AGCCTCCAAC CTCTCTTGGG
    CTTGTGCCTC ACCAGATCCG GGGATTTCTT TCACGGTTTG ATAATGTCCT TCCCGTCAGC
    CTGGCATTTG ACAAATGTAC AGCTTGTTCT TCCAAAGTTC TTGATCAATA TGAACGAGAA
    GGATTTAACT TCCTAGCCAA GGTGTTTAAT TCTTCACATT CCTTCTTAGA AGACTTGACT
    GGTCTTACAT TGCTGCATCA AGAAACCCAA GCTGCTGAGC ACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011531586.1
    CDS449..2533
    Translation

    Target ORF information:

    RefSeq Version XM_011533284.2
    Organism Homo sapiens(human)
    Definition Homo sapiens autophagy related 7 (ATG7), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011533284.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGCGGCAG CTACGGGGGA TCCTGGACTC TCTAAACTGC AGTTTGCCCC TTTTAGTAGT 
    GCCTTGGATG TTGGGTTTTG GCATGAGTTG ACCCAGAAGA AGCTGAACGA GTATCGGCTG
    GATGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAATG GTGACTCTGC TGGGCTGCCA
    GCTCGCTTAA CATTGGAGTT CAGTGCTTTT GACATGAGTG CTCCCACCCC AGCCCGTTGC
    TGCCCAGCTA TTGGAACACT GTATAACACC AACACACTCG AGTCTTTCAA GACTGCAGAT
    AAGAAGCTCC TTTTGGAACA AGCAGCAAAT GAGATATGGG AATCCATAAA ATCAGGCACT
    GCTCTTGAAA ACCCTGTACT CCTCAACAAG TTCCTCCTCT TGACATTTGC AGATCTAAAG
    AAGTACCACT TCTACTATTG GTTTTGCTAT CCTGCCCTCT GTCTTCCAGA GAGTTTACCT
    CTCATTCAGG GGCCAGTGGG TTTGGATCAA AGGTTTTCAC TAAAACAGAT TGAAGCACTA
    GAGTGTGCAT ATGATAATCT TTGTCAAACA GAAGGAGTCA CAGCTCTTCC TTACTTCTTA
    ATCAAGTATG ATGAGAACAT GGTGCTGGTT TCCTTGCTTA AACACTACAG TGATTTCTTC
    CAAGGTCAAA GGACGAAGAT AACAATTGGT GTATATGATC CCTGTAACTT AGCCCAGTAC
    CCTGGATGGC CTTTGAGGAA TTTTTTGGTC CTAGCAGCCC ACAGATGGAG TAGCAGTTTC
    CAGTCTGTTG AAGTTGTTTG CTTCCGTGAC CGTACCATGC AGGGGGCGAG AGACGTTGCC
    CACAGCATCA TCTTCGAAGT GAAGCTTCCA GAAATGGCAT TTAGCCCAGA TTGTCCTAAA
    GCAGTTGGAT GGGAAAAGAA CCAGAAAGGA GGCATGGGAC CAAGGATGGT GAACCTCAGT
    GAATGTATGG ACCCTAAAAG GTTAGCTGAG TCATCAGTGG ATCTAAATCT CAAACTGATG
    TGTTGGAGAT TGGTTCCTAC TTTAGACTTG GACAAGGTTG TGTCTGTCAA ATGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGCAATGTA GCTAGGACGT TGATGGGTTG GGGCGTGAGA
    CACATCACAT TTGTGGACAA TGCCAAGATC TCCTACTCCA ATCCTGTGAG GCAGCCTCTC
    TATGAGTTTG AAGATTGCCT AGGGGGTGGT AAGCCCAAGG CTCTGGCAGC AGCGGACCGG
    CTCCAGAAAA TATTCCCCGG TGTGAATGCC AGAGGATTCA ACATGAGCAT ACCTATGCCT
    GGGCATCCAG TGAACTTCTC CAGTGTCACT CTGGAGCAAG CCCGCAGAGA TGTGGAGCAA
    CTGGAGCAGC TCATCGAAAG CCATGATGTC GTCTTCCTAT TGATGGACAC CAGGGAGAGC
    CGGTGGCTTC CTGCCGTCAT TGCTGCAAGC AAGAGAAAGC TGGTCATCAA TGCTGCTTTG
    GGATTTGACA CATTTGTTGT CATGAGACAT GGTCTGAAGA AACCAAAGCA GCAAGGAGCT
    GGGGACTTGT GTCCAAACCA CCCTGTGGCA TCTGCTGACC TCCTGGGCTC ATCGCTTTTT
    GCCAACATCC CTGGTTACAA GCTTGGCTGC TACTTCTGCA ATGATGTGGT GGCCCCAGGA
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGTCGTCC AGGACTGGCC
    GTGATTGCAG GAGCCCTGGC CGTGGAATTG ATGGTATCTG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAT CGGATGAATG AGCCTCCAAC CTCTCTTGGG
    CTTGTGCCTC ACCAGATCCG GGGATTTCTT TCACGGTTTG ATAATGTCCT TCCCGTCAGC
    CTGGCATTTG ACAAATGTAC AGCTTGTTCT TCCAAAGTTC TTGATCAATA TGAACGAGAA
    GGATTTAACT TCCTAGCCAA GGTGTTTAAT TCTTCACATT CCTTCTTAGA AGACTTGACT
    GGTCTTACAT TGCTGCATCA AGAAACCCAA GCTGCTGAGC ACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu102116
    Clone ID Related Accession (Same CDS sequence) NM_001349236.2 , NM_001349236.1
    Accession Version NM_001349236.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1995bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-06-22
    Organism Homo sapiens(human)
    Product ubiquitin-like modifier-activating enzyme ATG7 isoform d
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC083855.3, AC020750.4, AC026185.4 and AC022001.5. On Jun 2, 2019 this sequence version replaced NM_001349236.1. Transcript Variant: This variant (8) encodes isoform d. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.86261.1, SRR1803616.142440.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGCGGCAG CTACGGGGGA TCCTGGACTC TCTAAACTGC AGTTTGCCCC TTTTAGTAGT 
    GCCTTGGATG TTGGGTTTTG GCATGAGTTG ACCCAGAAGA AGCTGAACGA GTATCGGCTG
    GATGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAATG GTGACTCTGC TGGGCTGCCA
    GCTCGCTTAA CATTGGAGTT CAGTGCTTTT GACATGAGTG CTCCCACCCC AGCCCGTTGC
    TGCCCAGCTA TTGGAACACT GTATAACACC AACACACTCG AGTCTTTCAA GACTGCAGAT
    AAGAAGCTCC TTTTGGAACA AGCAGCAAAT GAGATATGGG AATCCATAAA ATCAGGCACT
    GCTCTTGAAA ACCCTGTACT CCTCAACAAG TTCCTCCTCT TGACATTTGC AATTGAAGCA
    CTAGAGTGTG CATATGATAA TCTTTGTCAA ACAGAAGGAG TCACAGCTCT TCCTTACTTC
    TTAATCAAGT ATGATGAGAA CATGGTGCTG GTTTCCTTGC TTAAACACTA CAGTGATTTC
    TTCCAAGGTC AAAGGACGAA GATAACAATT GGTGTATATG ATCCCTGTAA CTTAGCCCAG
    TACCCTGGAT GGCCTTTGAG GAATTTTTTG GTCCTAGCAG CCCACAGATG GAGTAGCAGT
    TTCCAGTCTG TTGAAGTTGT TTGCTTCCGT GACCGTACCA TGCAGGGGGC GAGAGACGTT
    GCCCACAGCA TCATCTTCGA AGTGAAGCTT CCAGAAATGG CATTTAGCCC AGATTGTCCT
    AAAGCAGTTG GATGGGAAAA GAACCAGAAA GGAGGCATGG GACCAAGGAT GGTGAACCTC
    AGTGAATGTA TGGACCCTAA AAGGTTAGCT GAGTCATCAG TGGATCTAAA TCTCAAACTG
    ATGTGTTGGA GATTGGTTCC TACTTTAGAC TTGGACAAGG TTGTGTCTGT CAAATGTCTG
    CTGCTTGGAG CCGGCACCTT GGGTTGCAAT GTAGCTAGGA CGTTGATGGG TTGGGGCGTG
    AGACACATCA CATTTGTGGA CAATGCCAAG ATCTCCTACT CCAATCCTGT GAGGCAGCCT
    CTCTATGAGT TTGAAGATTG CCTAGGGGGT GGTAAGCCCA AGGCTCTGGC AGCAGCGGAC
    CGGCTCCAGA AAATATTCCC CGGTGTGAAT GCCAGAGGAT TCAACATGAG CATACCTATG
    CCTGGGCATC CAGTGAACTT CTCCAGTGTC ACTCTGGAGC AAGCCCGCAG AGATGTGGAG
    CAACTGGAGC AGCTCATCGA AAGCCATGAT GTCGTCTTCC TATTGATGGA CACCAGGGAG
    AGCCGGTGGC TTCCTGCCGT CATTGCTGCA AGCAAGAGAA AGCTGGTCAT CAATGCTGCT
    TTGGGATTTG ACACATTTGT TGTCATGAGA CATGGTCTGA AGAAACCAAA GCAGCAAGGA
    GCTGGGGACT TGTGTCCAAA CCACCCTGTG GCATCTGCTG ACCTCCTGGG CTCATCGCTT
    TTTGCCAACA TCCCTGGTTA CAAGCTTGGC TGCTACTTCT GCAATGATGT GGTGGCCCCA
    GGAGATTCAA CCAGAGACCG GACCTTGGAC CAGCAGTGCA CTGTGAGTCG TCCAGGACTG
    GCCGTGATTG CAGGAGCCCT GGCCGTGGAA TTGATGGTAT CTGTTTTGCA GCATCCAGAA
    GGGGGCTATG CCATTGCCAG CAGCAGTGAC GATCGGATGA ATGAGCCTCC AACCTCTCTT
    GGGCTTGTGC CTCACCAGAT CCGGGGATTT CTTTCACGGT TTGATAATGT CCTTCCCGTC
    AGCCTGGCAT TTGACAAATG TACAGCTTGT TCTTCCAAAG TTCTTGATCA ATATGAACGA
    GAAGGATTTA ACTTCCTAGC CAAGGTGTTT AATTCTTCAC ATTCCTTCTT AGAAGACTTG
    ACTGGTCTTA CATTGCTGCA TCAAGAAACC CAAGCTGCTG AGATCTGGGA CATGAGCGAT
    GATGAGACCA TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001336165.1
    CDS45..2039
    Translation

    Target ORF information:

    RefSeq Version NM_001349236.2
    Organism Homo sapiens(human)
    Definition Homo sapiens autophagy related 7 (ATG7), transcript variant 8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001349236.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGCGGCAG CTACGGGGGA TCCTGGACTC TCTAAACTGC AGTTTGCCCC TTTTAGTAGT 
    GCCTTGGATG TTGGGTTTTG GCATGAGTTG ACCCAGAAGA AGCTGAACGA GTATCGGCTG
    GATGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAATG GTGACTCTGC TGGGCTGCCA
    GCTCGCTTAA CATTGGAGTT CAGTGCTTTT GACATGAGTG CTCCCACCCC AGCCCGTTGC
    TGCCCAGCTA TTGGAACACT GTATAACACC AACACACTCG AGTCTTTCAA GACTGCAGAT
    AAGAAGCTCC TTTTGGAACA AGCAGCAAAT GAGATATGGG AATCCATAAA ATCAGGCACT
    GCTCTTGAAA ACCCTGTACT CCTCAACAAG TTCCTCCTCT TGACATTTGC AATTGAAGCA
    CTAGAGTGTG CATATGATAA TCTTTGTCAA ACAGAAGGAG TCACAGCTCT TCCTTACTTC
    TTAATCAAGT ATGATGAGAA CATGGTGCTG GTTTCCTTGC TTAAACACTA CAGTGATTTC
    TTCCAAGGTC AAAGGACGAA GATAACAATT GGTGTATATG ATCCCTGTAA CTTAGCCCAG
    TACCCTGGAT GGCCTTTGAG GAATTTTTTG GTCCTAGCAG CCCACAGATG GAGTAGCAGT
    TTCCAGTCTG TTGAAGTTGT TTGCTTCCGT GACCGTACCA TGCAGGGGGC GAGAGACGTT
    GCCCACAGCA TCATCTTCGA AGTGAAGCTT CCAGAAATGG CATTTAGCCC AGATTGTCCT
    AAAGCAGTTG GATGGGAAAA GAACCAGAAA GGAGGCATGG GACCAAGGAT GGTGAACCTC
    AGTGAATGTA TGGACCCTAA AAGGTTAGCT GAGTCATCAG TGGATCTAAA TCTCAAACTG
    ATGTGTTGGA GATTGGTTCC TACTTTAGAC TTGGACAAGG TTGTGTCTGT CAAATGTCTG
    CTGCTTGGAG CCGGCACCTT GGGTTGCAAT GTAGCTAGGA CGTTGATGGG TTGGGGCGTG
    AGACACATCA CATTTGTGGA CAATGCCAAG ATCTCCTACT CCAATCCTGT GAGGCAGCCT
    CTCTATGAGT TTGAAGATTG CCTAGGGGGT GGTAAGCCCA AGGCTCTGGC AGCAGCGGAC
    CGGCTCCAGA AAATATTCCC CGGTGTGAAT GCCAGAGGAT TCAACATGAG CATACCTATG
    CCTGGGCATC CAGTGAACTT CTCCAGTGTC ACTCTGGAGC AAGCCCGCAG AGATGTGGAG
    CAACTGGAGC AGCTCATCGA AAGCCATGAT GTCGTCTTCC TATTGATGGA CACCAGGGAG
    AGCCGGTGGC TTCCTGCCGT CATTGCTGCA AGCAAGAGAA AGCTGGTCAT CAATGCTGCT
    TTGGGATTTG ACACATTTGT TGTCATGAGA CATGGTCTGA AGAAACCAAA GCAGCAAGGA
    GCTGGGGACT TGTGTCCAAA CCACCCTGTG GCATCTGCTG ACCTCCTGGG CTCATCGCTT
    TTTGCCAACA TCCCTGGTTA CAAGCTTGGC TGCTACTTCT GCAATGATGT GGTGGCCCCA
    GGAGATTCAA CCAGAGACCG GACCTTGGAC CAGCAGTGCA CTGTGAGTCG TCCAGGACTG
    GCCGTGATTG CAGGAGCCCT GGCCGTGGAA TTGATGGTAT CTGTTTTGCA GCATCCAGAA
    GGGGGCTATG CCATTGCCAG CAGCAGTGAC GATCGGATGA ATGAGCCTCC AACCTCTCTT
    GGGCTTGTGC CTCACCAGAT CCGGGGATTT CTTTCACGGT TTGATAATGT CCTTCCCGTC
    AGCCTGGCAT TTGACAAATG TACAGCTTGT TCTTCCAAAG TTCTTGATCA ATATGAACGA
    GAAGGATTTA ACTTCCTAGC CAAGGTGTTT AATTCTTCAC ATTCCTTCTT AGAAGACTTG
    ACTGGTCTTA CATTGCTGCA TCAAGAAACC CAAGCTGCTG AGATCTGGGA CATGAGCGAT
    GATGAGACCA TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu102116
    Clone ID Related Accession (Same CDS sequence) NM_001349236.2 , NM_001349236.1
    Accession Version NM_001349236.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1995bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-02
    Organism Homo sapiens(human)
    Product ubiquitin-like modifier-activating enzyme ATG7 isoform d
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC083855.3, AC020750.4, AC026185.4 and AC022001.5. Transcript Variant: This variant (8) encodes isoform d. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.142440.1, SRR1803617.86261.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGCGGCAG CTACGGGGGA TCCTGGACTC TCTAAACTGC AGTTTGCCCC TTTTAGTAGT 
    GCCTTGGATG TTGGGTTTTG GCATGAGTTG ACCCAGAAGA AGCTGAACGA GTATCGGCTG
    GATGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAATG GTGACTCTGC TGGGCTGCCA
    GCTCGCTTAA CATTGGAGTT CAGTGCTTTT GACATGAGTG CTCCCACCCC AGCCCGTTGC
    TGCCCAGCTA TTGGAACACT GTATAACACC AACACACTCG AGTCTTTCAA GACTGCAGAT
    AAGAAGCTCC TTTTGGAACA AGCAGCAAAT GAGATATGGG AATCCATAAA ATCAGGCACT
    GCTCTTGAAA ACCCTGTACT CCTCAACAAG TTCCTCCTCT TGACATTTGC AATTGAAGCA
    CTAGAGTGTG CATATGATAA TCTTTGTCAA ACAGAAGGAG TCACAGCTCT TCCTTACTTC
    TTAATCAAGT ATGATGAGAA CATGGTGCTG GTTTCCTTGC TTAAACACTA CAGTGATTTC
    TTCCAAGGTC AAAGGACGAA GATAACAATT GGTGTATATG ATCCCTGTAA CTTAGCCCAG
    TACCCTGGAT GGCCTTTGAG GAATTTTTTG GTCCTAGCAG CCCACAGATG GAGTAGCAGT
    TTCCAGTCTG TTGAAGTTGT TTGCTTCCGT GACCGTACCA TGCAGGGGGC GAGAGACGTT
    GCCCACAGCA TCATCTTCGA AGTGAAGCTT CCAGAAATGG CATTTAGCCC AGATTGTCCT
    AAAGCAGTTG GATGGGAAAA GAACCAGAAA GGAGGCATGG GACCAAGGAT GGTGAACCTC
    AGTGAATGTA TGGACCCTAA AAGGTTAGCT GAGTCATCAG TGGATCTAAA TCTCAAACTG
    ATGTGTTGGA GATTGGTTCC TACTTTAGAC TTGGACAAGG TTGTGTCTGT CAAATGTCTG
    CTGCTTGGAG CCGGCACCTT GGGTTGCAAT GTAGCTAGGA CGTTGATGGG TTGGGGCGTG
    AGACACATCA CATTTGTGGA CAATGCCAAG ATCTCCTACT CCAATCCTGT GAGGCAGCCT
    CTCTATGAGT TTGAAGATTG CCTAGGGGGT GGTAAGCCCA AGGCTCTGGC AGCAGCGGAC
    CGGCTCCAGA AAATATTCCC CGGTGTGAAT GCCAGAGGAT TCAACATGAG CATACCTATG
    CCTGGGCATC CAGTGAACTT CTCCAGTGTC ACTCTGGAGC AAGCCCGCAG AGATGTGGAG
    CAACTGGAGC AGCTCATCGA AAGCCATGAT GTCGTCTTCC TATTGATGGA CACCAGGGAG
    AGCCGGTGGC TTCCTGCCGT CATTGCTGCA AGCAAGAGAA AGCTGGTCAT CAATGCTGCT
    TTGGGATTTG ACACATTTGT TGTCATGAGA CATGGTCTGA AGAAACCAAA GCAGCAAGGA
    GCTGGGGACT TGTGTCCAAA CCACCCTGTG GCATCTGCTG ACCTCCTGGG CTCATCGCTT
    TTTGCCAACA TCCCTGGTTA CAAGCTTGGC TGCTACTTCT GCAATGATGT GGTGGCCCCA
    GGAGATTCAA CCAGAGACCG GACCTTGGAC CAGCAGTGCA CTGTGAGTCG TCCAGGACTG
    GCCGTGATTG CAGGAGCCCT GGCCGTGGAA TTGATGGTAT CTGTTTTGCA GCATCCAGAA
    GGGGGCTATG CCATTGCCAG CAGCAGTGAC GATCGGATGA ATGAGCCTCC AACCTCTCTT
    GGGCTTGTGC CTCACCAGAT CCGGGGATTT CTTTCACGGT TTGATAATGT CCTTCCCGTC
    AGCCTGGCAT TTGACAAATG TACAGCTTGT TCTTCCAAAG TTCTTGATCA ATATGAACGA
    GAAGGATTTA ACTTCCTAGC CAAGGTGTTT AATTCTTCAC ATTCCTTCTT AGAAGACTTG
    ACTGGTCTTA CATTGCTGCA TCAAGAAACC CAAGCTGCTG AGATCTGGGA CATGAGCGAT
    GATGAGACCA TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001336165.1
    CDS118..2112
    Translation

    Target ORF information:

    RefSeq Version NM_001349236.1
    Organism Homo sapiens(human)
    Definition Homo sapiens autophagy related 7 (ATG7), transcript variant 8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001349236.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGCGGCAG CTACGGGGGA TCCTGGACTC TCTAAACTGC AGTTTGCCCC TTTTAGTAGT 
    GCCTTGGATG TTGGGTTTTG GCATGAGTTG ACCCAGAAGA AGCTGAACGA GTATCGGCTG
    GATGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAATG GTGACTCTGC TGGGCTGCCA
    GCTCGCTTAA CATTGGAGTT CAGTGCTTTT GACATGAGTG CTCCCACCCC AGCCCGTTGC
    TGCCCAGCTA TTGGAACACT GTATAACACC AACACACTCG AGTCTTTCAA GACTGCAGAT
    AAGAAGCTCC TTTTGGAACA AGCAGCAAAT GAGATATGGG AATCCATAAA ATCAGGCACT
    GCTCTTGAAA ACCCTGTACT CCTCAACAAG TTCCTCCTCT TGACATTTGC AATTGAAGCA
    CTAGAGTGTG CATATGATAA TCTTTGTCAA ACAGAAGGAG TCACAGCTCT TCCTTACTTC
    TTAATCAAGT ATGATGAGAA CATGGTGCTG GTTTCCTTGC TTAAACACTA CAGTGATTTC
    TTCCAAGGTC AAAGGACGAA GATAACAATT GGTGTATATG ATCCCTGTAA CTTAGCCCAG
    TACCCTGGAT GGCCTTTGAG GAATTTTTTG GTCCTAGCAG CCCACAGATG GAGTAGCAGT
    TTCCAGTCTG TTGAAGTTGT TTGCTTCCGT GACCGTACCA TGCAGGGGGC GAGAGACGTT
    GCCCACAGCA TCATCTTCGA AGTGAAGCTT CCAGAAATGG CATTTAGCCC AGATTGTCCT
    AAAGCAGTTG GATGGGAAAA GAACCAGAAA GGAGGCATGG GACCAAGGAT GGTGAACCTC
    AGTGAATGTA TGGACCCTAA AAGGTTAGCT GAGTCATCAG TGGATCTAAA TCTCAAACTG
    ATGTGTTGGA GATTGGTTCC TACTTTAGAC TTGGACAAGG TTGTGTCTGT CAAATGTCTG
    CTGCTTGGAG CCGGCACCTT GGGTTGCAAT GTAGCTAGGA CGTTGATGGG TTGGGGCGTG
    AGACACATCA CATTTGTGGA CAATGCCAAG ATCTCCTACT CCAATCCTGT GAGGCAGCCT
    CTCTATGAGT TTGAAGATTG CCTAGGGGGT GGTAAGCCCA AGGCTCTGGC AGCAGCGGAC
    CGGCTCCAGA AAATATTCCC CGGTGTGAAT GCCAGAGGAT TCAACATGAG CATACCTATG
    CCTGGGCATC CAGTGAACTT CTCCAGTGTC ACTCTGGAGC AAGCCCGCAG AGATGTGGAG
    CAACTGGAGC AGCTCATCGA AAGCCATGAT GTCGTCTTCC TATTGATGGA CACCAGGGAG
    AGCCGGTGGC TTCCTGCCGT CATTGCTGCA AGCAAGAGAA AGCTGGTCAT CAATGCTGCT
    TTGGGATTTG ACACATTTGT TGTCATGAGA CATGGTCTGA AGAAACCAAA GCAGCAAGGA
    GCTGGGGACT TGTGTCCAAA CCACCCTGTG GCATCTGCTG ACCTCCTGGG CTCATCGCTT
    TTTGCCAACA TCCCTGGTTA CAAGCTTGGC TGCTACTTCT GCAATGATGT GGTGGCCCCA
    GGAGATTCAA CCAGAGACCG GACCTTGGAC CAGCAGTGCA CTGTGAGTCG TCCAGGACTG
    GCCGTGATTG CAGGAGCCCT GGCCGTGGAA TTGATGGTAT CTGTTTTGCA GCATCCAGAA
    GGGGGCTATG CCATTGCCAG CAGCAGTGAC GATCGGATGA ATGAGCCTCC AACCTCTCTT
    GGGCTTGTGC CTCACCAGAT CCGGGGATTT CTTTCACGGT TTGATAATGT CCTTCCCGTC
    AGCCTGGCAT TTGACAAATG TACAGCTTGT TCTTCCAAAG TTCTTGATCA ATATGAACGA
    GAAGGATTTA ACTTCCTAGC CAAGGTGTTT AATTCTTCAC ATTCCTTCTT AGAAGACTTG
    ACTGGTCTTA CATTGCTGCA TCAAGAAACC CAAGCTGCTG AGATCTGGGA CATGAGCGAT
    GATGAGACCA TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu102196
    Clone ID Related Accession (Same CDS sequence) NM_001349237.2 , NM_001349237.1
    Accession Version NM_001349237.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1953bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-06-22
    Organism Homo sapiens(human)
    Product ubiquitin-like modifier-activating enzyme ATG7 isoform e
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC083855.3, AC020750.4, AC026185.4 and AC022001.5. On Jun 2, 2019 this sequence version replaced NM_001349237.1. Transcript Variant: This variant (9) encodes isoform e. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.210117.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGCGGCAG CTACGGGGGA TCCTGGACTC TCTAAACTGC AGTTTGCCCC TTTTAGTAGT 
    GCCTTGGATG TTGGGTTTTG GCATGAGTTG ACCCAGAAGA AGCTGAACGA GTATCGGCTG
    GATGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAATG GTGACTCTGC TGGGCTGCCA
    GCTCGCTTAA CATTGGAGTT CAGTGCTTTT GACATGAGTG CTCCCACCCC AGCCCGTTGC
    TGCCCAGCTA TTGGAACACT GTATAACACC AACACACTCG AGTCTTTCAA GACTGCAGAT
    AAGAAGCTCC TTTTGGAACA AGCAGCAAAT GAGATATGGG AATCCATAAA ATCAGGCACT
    GCTCTTGAAA ACCCTGTACT CCTCAACAAG TTCCTCCTCT TGACATTTGC AGATCTAAAG
    AAGTACCACT TCTACTATTG GTTTTGCTAT CCTGCCCTCT GTCTTCCAGA GAGTTTACCT
    CTCATTCAGG GGCCAGTGGG TTTGGATCAA AGGTTTTCAC TAAAACAGAT TGAAGCACTA
    GAGTGTGCAT ATGATAATCT TTGTCAAACA GAAGGAGTCA CAGCTCTTCC TTACTTCTTA
    ATCAAGTATG ATGAGAACAT GGTGCTGGTT TCCTTGCTTA AACACTACAG TGATTTCTTC
    CAAGGTCAAA GGACGAAGAT AACAATTGGT GTATATGATC CCTGTAACTT AGCCCAGTAC
    CCTGGATGGC CTTTGAGGAA TTTTTTGGTC CTAGCAGCCC ACAGATGGAG TAGCAGTTTC
    CAGTCTGTTG AAGTTGTTTG CTTCCGTGAC CGTACCATGC AGGGGGCGAG AGACGTTGCC
    CACAGCATCA TCTTCGAAGT GAAGCTTCCA GAAATGGCAT TTAGCCCAGA TTGTCCTAAA
    GCAGTTGGAT GGGAAAAGAA CCAGAAAGGA GGCATGGGAC CAAGGATGGT GAACCTCAGT
    GAATGTATGG ACCCTAAAAG GTTAGCTGAG TCATCAGTGG ATCTAAATCT CAAACTGATG
    TGTTGGAGAT TGGTTCCTAC TTTAGACTTG GACAAGGTTG TGTCTGTCAA ATGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGCAATGTA GCTAGGACGT TGATGAATGC CAGAGGATTC
    AACATGAGCA TACCTATGCC TGGGCATCCA GTGAACTTCT CCAGTGTCAC TCTGGAGCAA
    GCCCGCAGAG ATGTGGAGCA ACTGGAGCAG CTCATCGAAA GCCATGATGT CGTCTTCCTA
    TTGATGGACA CCAGGGAGAG CCGGTGGCTT CCTGCCGTCA TTGCTGCAAG CAAGAGAAAG
    CTGGTCATCA ATGCTGCTTT GGGATTTGAC ACATTTGTTG TCATGAGACA TGGTCTGAAG
    AAACCAAAGC AGCAAGGAGC TGGGGACTTG TGTCCAAACC ACCCTGTGGC ATCTGCTGAC
    CTCCTGGGCT CATCGCTTTT TGCCAACATC CCTGGTTACA AGCTTGGCTG CTACTTCTGC
    AATGATGTGG TGGCCCCAGG AGATTCAACC AGAGACCGGA CCTTGGACCA GCAGTGCACT
    GTGAGTCGTC CAGGACTGGC CGTGATTGCA GGAGCCCTGG CCGTGGAATT GATGGTATCT
    GTTTTGCAGC ATCCAGAAGG GGGCTATGCC ATTGCCAGCA GCAGTGACGA TCGGATGAAT
    GAGCCTCCAA CCTCTCTTGG GCTTGTGCCT CACCAGATCC GGGGATTTCT TTCACGGTTT
    GATAATGTCC TTCCCGTCAG CCTGGCATTT GACAAATGTA CAGCTTGTTC TTCCAAAGTT
    CTTGATCAAT ATGAACGAGA AGGATTTAAC TTCCTAGCCA AGGTGTTTAA TTCTTCACAT
    TCCTTCTTAG AAGACTTGAC TGGTCTTACA TTGCTGCATC AAGAAACCCA AGCTGCTGAG
    ATCTGGGACA TGAGCGATGA TGAGACCATC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001336166.1
    CDS45..1997
    Translation

    Target ORF information:

    RefSeq Version NM_001349237.2
    Organism Homo sapiens(human)
    Definition Homo sapiens autophagy related 7 (ATG7), transcript variant 9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001349237.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGCGGCAG CTACGGGGGA TCCTGGACTC TCTAAACTGC AGTTTGCCCC TTTTAGTAGT 
    GCCTTGGATG TTGGGTTTTG GCATGAGTTG ACCCAGAAGA AGCTGAACGA GTATCGGCTG
    GATGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAATG GTGACTCTGC TGGGCTGCCA
    GCTCGCTTAA CATTGGAGTT CAGTGCTTTT GACATGAGTG CTCCCACCCC AGCCCGTTGC
    TGCCCAGCTA TTGGAACACT GTATAACACC AACACACTCG AGTCTTTCAA GACTGCAGAT
    AAGAAGCTCC TTTTGGAACA AGCAGCAAAT GAGATATGGG AATCCATAAA ATCAGGCACT
    GCTCTTGAAA ACCCTGTACT CCTCAACAAG TTCCTCCTCT TGACATTTGC AGATCTAAAG
    AAGTACCACT TCTACTATTG GTTTTGCTAT CCTGCCCTCT GTCTTCCAGA GAGTTTACCT
    CTCATTCAGG GGCCAGTGGG TTTGGATCAA AGGTTTTCAC TAAAACAGAT TGAAGCACTA
    GAGTGTGCAT ATGATAATCT TTGTCAAACA GAAGGAGTCA CAGCTCTTCC TTACTTCTTA
    ATCAAGTATG ATGAGAACAT GGTGCTGGTT TCCTTGCTTA AACACTACAG TGATTTCTTC
    CAAGGTCAAA GGACGAAGAT AACAATTGGT GTATATGATC CCTGTAACTT AGCCCAGTAC
    CCTGGATGGC CTTTGAGGAA TTTTTTGGTC CTAGCAGCCC ACAGATGGAG TAGCAGTTTC
    CAGTCTGTTG AAGTTGTTTG CTTCCGTGAC CGTACCATGC AGGGGGCGAG AGACGTTGCC
    CACAGCATCA TCTTCGAAGT GAAGCTTCCA GAAATGGCAT TTAGCCCAGA TTGTCCTAAA
    GCAGTTGGAT GGGAAAAGAA CCAGAAAGGA GGCATGGGAC CAAGGATGGT GAACCTCAGT
    GAATGTATGG ACCCTAAAAG GTTAGCTGAG TCATCAGTGG ATCTAAATCT CAAACTGATG
    TGTTGGAGAT TGGTTCCTAC TTTAGACTTG GACAAGGTTG TGTCTGTCAA ATGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGCAATGTA GCTAGGACGT TGATGAATGC CAGAGGATTC
    AACATGAGCA TACCTATGCC TGGGCATCCA GTGAACTTCT CCAGTGTCAC TCTGGAGCAA
    GCCCGCAGAG ATGTGGAGCA ACTGGAGCAG CTCATCGAAA GCCATGATGT CGTCTTCCTA
    TTGATGGACA CCAGGGAGAG CCGGTGGCTT CCTGCCGTCA TTGCTGCAAG CAAGAGAAAG
    CTGGTCATCA ATGCTGCTTT GGGATTTGAC ACATTTGTTG TCATGAGACA TGGTCTGAAG
    AAACCAAAGC AGCAAGGAGC TGGGGACTTG TGTCCAAACC ACCCTGTGGC ATCTGCTGAC
    CTCCTGGGCT CATCGCTTTT TGCCAACATC CCTGGTTACA AGCTTGGCTG CTACTTCTGC
    AATGATGTGG TGGCCCCAGG AGATTCAACC AGAGACCGGA CCTTGGACCA GCAGTGCACT
    GTGAGTCGTC CAGGACTGGC CGTGATTGCA GGAGCCCTGG CCGTGGAATT GATGGTATCT
    GTTTTGCAGC ATCCAGAAGG GGGCTATGCC ATTGCCAGCA GCAGTGACGA TCGGATGAAT
    GAGCCTCCAA CCTCTCTTGG GCTTGTGCCT CACCAGATCC GGGGATTTCT TTCACGGTTT
    GATAATGTCC TTCCCGTCAG CCTGGCATTT GACAAATGTA CAGCTTGTTC TTCCAAAGTT
    CTTGATCAAT ATGAACGAGA AGGATTTAAC TTCCTAGCCA AGGTGTTTAA TTCTTCACAT
    TCCTTCTTAG AAGACTTGAC TGGTCTTACA TTGCTGCATC AAGAAACCCA AGCTGCTGAG
    ATCTGGGACA TGAGCGATGA TGAGACCATC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu102196
    Clone ID Related Accession (Same CDS sequence) NM_001349237.2 , NM_001349237.1
    Accession Version NM_001349237.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1953bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-02
    Organism Homo sapiens(human)
    Product ubiquitin-like modifier-activating enzyme ATG7 isoform e
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC083855.3, AC020750.4, AC026185.4 and AC022001.5. On Mar 9, 2017 this sequence version replaced XM_017005552.1. Transcript Variant: This variant (9) encodes isoform e. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.210117.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGCGGCAG CTACGGGGGA TCCTGGACTC TCTAAACTGC AGTTTGCCCC TTTTAGTAGT 
    GCCTTGGATG TTGGGTTTTG GCATGAGTTG ACCCAGAAGA AGCTGAACGA GTATCGGCTG
    GATGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAATG GTGACTCTGC TGGGCTGCCA
    GCTCGCTTAA CATTGGAGTT CAGTGCTTTT GACATGAGTG CTCCCACCCC AGCCCGTTGC
    TGCCCAGCTA TTGGAACACT GTATAACACC AACACACTCG AGTCTTTCAA GACTGCAGAT
    AAGAAGCTCC TTTTGGAACA AGCAGCAAAT GAGATATGGG AATCCATAAA ATCAGGCACT
    GCTCTTGAAA ACCCTGTACT CCTCAACAAG TTCCTCCTCT TGACATTTGC AGATCTAAAG
    AAGTACCACT TCTACTATTG GTTTTGCTAT CCTGCCCTCT GTCTTCCAGA GAGTTTACCT
    CTCATTCAGG GGCCAGTGGG TTTGGATCAA AGGTTTTCAC TAAAACAGAT TGAAGCACTA
    GAGTGTGCAT ATGATAATCT TTGTCAAACA GAAGGAGTCA CAGCTCTTCC TTACTTCTTA
    ATCAAGTATG ATGAGAACAT GGTGCTGGTT TCCTTGCTTA AACACTACAG TGATTTCTTC
    CAAGGTCAAA GGACGAAGAT AACAATTGGT GTATATGATC CCTGTAACTT AGCCCAGTAC
    CCTGGATGGC CTTTGAGGAA TTTTTTGGTC CTAGCAGCCC ACAGATGGAG TAGCAGTTTC
    CAGTCTGTTG AAGTTGTTTG CTTCCGTGAC CGTACCATGC AGGGGGCGAG AGACGTTGCC
    CACAGCATCA TCTTCGAAGT GAAGCTTCCA GAAATGGCAT TTAGCCCAGA TTGTCCTAAA
    GCAGTTGGAT GGGAAAAGAA CCAGAAAGGA GGCATGGGAC CAAGGATGGT GAACCTCAGT
    GAATGTATGG ACCCTAAAAG GTTAGCTGAG TCATCAGTGG ATCTAAATCT CAAACTGATG
    TGTTGGAGAT TGGTTCCTAC TTTAGACTTG GACAAGGTTG TGTCTGTCAA ATGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGCAATGTA GCTAGGACGT TGATGAATGC CAGAGGATTC
    AACATGAGCA TACCTATGCC TGGGCATCCA GTGAACTTCT CCAGTGTCAC TCTGGAGCAA
    GCCCGCAGAG ATGTGGAGCA ACTGGAGCAG CTCATCGAAA GCCATGATGT CGTCTTCCTA
    TTGATGGACA CCAGGGAGAG CCGGTGGCTT CCTGCCGTCA TTGCTGCAAG CAAGAGAAAG
    CTGGTCATCA ATGCTGCTTT GGGATTTGAC ACATTTGTTG TCATGAGACA TGGTCTGAAG
    AAACCAAAGC AGCAAGGAGC TGGGGACTTG TGTCCAAACC ACCCTGTGGC ATCTGCTGAC
    CTCCTGGGCT CATCGCTTTT TGCCAACATC CCTGGTTACA AGCTTGGCTG CTACTTCTGC
    AATGATGTGG TGGCCCCAGG AGATTCAACC AGAGACCGGA CCTTGGACCA GCAGTGCACT
    GTGAGTCGTC CAGGACTGGC CGTGATTGCA GGAGCCCTGG CCGTGGAATT GATGGTATCT
    GTTTTGCAGC ATCCAGAAGG GGGCTATGCC ATTGCCAGCA GCAGTGACGA TCGGATGAAT
    GAGCCTCCAA CCTCTCTTGG GCTTGTGCCT CACCAGATCC GGGGATTTCT TTCACGGTTT
    GATAATGTCC TTCCCGTCAG CCTGGCATTT GACAAATGTA CAGCTTGTTC TTCCAAAGTT
    CTTGATCAAT ATGAACGAGA AGGATTTAAC TTCCTAGCCA AGGTGTTTAA TTCTTCACAT
    TCCTTCTTAG AAGACTTGAC TGGTCTTACA TTGCTGCATC AAGAAACCCA AGCTGCTGAG
    ATCTGGGACA TGAGCGATGA TGAGACCATC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001336166.1
    CDS118..2070
    Translation

    Target ORF information:

    RefSeq Version NM_001349237.1
    Organism Homo sapiens(human)
    Definition Homo sapiens autophagy related 7 (ATG7), transcript variant 9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001349237.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGCGGCAG CTACGGGGGA TCCTGGACTC TCTAAACTGC AGTTTGCCCC TTTTAGTAGT 
    GCCTTGGATG TTGGGTTTTG GCATGAGTTG ACCCAGAAGA AGCTGAACGA GTATCGGCTG
    GATGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAATG GTGACTCTGC TGGGCTGCCA
    GCTCGCTTAA CATTGGAGTT CAGTGCTTTT GACATGAGTG CTCCCACCCC AGCCCGTTGC
    TGCCCAGCTA TTGGAACACT GTATAACACC AACACACTCG AGTCTTTCAA GACTGCAGAT
    AAGAAGCTCC TTTTGGAACA AGCAGCAAAT GAGATATGGG AATCCATAAA ATCAGGCACT
    GCTCTTGAAA ACCCTGTACT CCTCAACAAG TTCCTCCTCT TGACATTTGC AGATCTAAAG
    AAGTACCACT TCTACTATTG GTTTTGCTAT CCTGCCCTCT GTCTTCCAGA GAGTTTACCT
    CTCATTCAGG GGCCAGTGGG TTTGGATCAA AGGTTTTCAC TAAAACAGAT TGAAGCACTA
    GAGTGTGCAT ATGATAATCT TTGTCAAACA GAAGGAGTCA CAGCTCTTCC TTACTTCTTA
    ATCAAGTATG ATGAGAACAT GGTGCTGGTT TCCTTGCTTA AACACTACAG TGATTTCTTC
    CAAGGTCAAA GGACGAAGAT AACAATTGGT GTATATGATC CCTGTAACTT AGCCCAGTAC
    CCTGGATGGC CTTTGAGGAA TTTTTTGGTC CTAGCAGCCC ACAGATGGAG TAGCAGTTTC
    CAGTCTGTTG AAGTTGTTTG CTTCCGTGAC CGTACCATGC AGGGGGCGAG AGACGTTGCC
    CACAGCATCA TCTTCGAAGT GAAGCTTCCA GAAATGGCAT TTAGCCCAGA TTGTCCTAAA
    GCAGTTGGAT GGGAAAAGAA CCAGAAAGGA GGCATGGGAC CAAGGATGGT GAACCTCAGT
    GAATGTATGG ACCCTAAAAG GTTAGCTGAG TCATCAGTGG ATCTAAATCT CAAACTGATG
    TGTTGGAGAT TGGTTCCTAC TTTAGACTTG GACAAGGTTG TGTCTGTCAA ATGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGCAATGTA GCTAGGACGT TGATGAATGC CAGAGGATTC
    AACATGAGCA TACCTATGCC TGGGCATCCA GTGAACTTCT CCAGTGTCAC TCTGGAGCAA
    GCCCGCAGAG ATGTGGAGCA ACTGGAGCAG CTCATCGAAA GCCATGATGT CGTCTTCCTA
    TTGATGGACA CCAGGGAGAG CCGGTGGCTT CCTGCCGTCA TTGCTGCAAG CAAGAGAAAG
    CTGGTCATCA ATGCTGCTTT GGGATTTGAC ACATTTGTTG TCATGAGACA TGGTCTGAAG
    AAACCAAAGC AGCAAGGAGC TGGGGACTTG TGTCCAAACC ACCCTGTGGC ATCTGCTGAC
    CTCCTGGGCT CATCGCTTTT TGCCAACATC CCTGGTTACA AGCTTGGCTG CTACTTCTGC
    AATGATGTGG TGGCCCCAGG AGATTCAACC AGAGACCGGA CCTTGGACCA GCAGTGCACT
    GTGAGTCGTC CAGGACTGGC CGTGATTGCA GGAGCCCTGG CCGTGGAATT GATGGTATCT
    GTTTTGCAGC ATCCAGAAGG GGGCTATGCC ATTGCCAGCA GCAGTGACGA TCGGATGAAT
    GAGCCTCCAA CCTCTCTTGG GCTTGTGCCT CACCAGATCC GGGGATTTCT TTCACGGTTT
    GATAATGTCC TTCCCGTCAG CCTGGCATTT GACAAATGTA CAGCTTGTTC TTCCAAAGTT
    CTTGATCAAT ATGAACGAGA AGGATTTAAC TTCCTAGCCA AGGTGTTTAA TTCTTCACAT
    TCCTTCTTAG AAGACTTGAC TGGTCTTACA TTGCTGCATC AAGAAACCCA AGCTGCTGAG
    ATCTGGGACA TGAGCGATGA TGAGACCATC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu93734
    Clone ID Related Accession (Same CDS sequence) XM_017005547.2 , XM_017005547.1
    Accession Version XM_017005547.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2106bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product ubiquitin-like modifier-activating enzyme ATG7 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017005547.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGCGGCAG CTACGGGGGA TCCTGGACTC TCTAAACTGC AGTTTGCCCC TTTTAGTAGT 
    GCCTTGGATG TTGGGTTTTG GCATGAGTTG ACCCAGAAGA AGCTGAACGA GTATCGGCTG
    GATGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAATG GTGACTCTGC TGGGCTGCCA
    GCTCGCTTAA CATTGGAGTT CAGTGCTTTT GACATGAGTG CTCCCACCCC AGCCCGTTGC
    TGCCCAGCTA TTGGAACACT GTATAACACC AACACACTCG AGTCTTTCAA GACTGCAGAT
    AAGAAGCTCC TTTTGGAACA AGCAGCAAAT GAGATATGGG AATCCATAAA ATCAGGCACT
    GCTCTTGAAA ACCCTGTACT CCTCAACAAG TTCCTCCTCT TGACATTTGC AGATCTAAAG
    AAGTACCACT TCTACTATTG GTTTTGCTAT CCTGCCCTCT GTCTTCCAGA GAGTTTACCT
    CTCATTCAGG GGCCAGTGGG TTTGGATCAA AGGTTTTCAC TAAAACAGAT TGAAGCACTA
    GAGTGTGCAT ATGATAATCT TTGTCAAACA GAAGGAGTCA CAGCTCTTCC TTACTTCTTA
    ATCAAGTATG ATGAGAACAT GGTGCTGGTT TCCTTGCTTA AACACTACAG TGATTTCTTC
    CAAGGTCAAA GGACGAAGAT AACAATTGGT GTATATGATC CCTGTAACTT AGCCCAGTAC
    CCTGGATGGC CTTTGAGGAA TTTTTTGGTC CTAGCAGCCC ACAGATGGAG TAGCAGTTTC
    CAGTCTGTTG AAGTTGTTTG CTTCCGTGAC CGTACCATGC AGGGGGCGAG AGACGTTGCC
    CACAGCATCA TCTTCGAAGT GAAGCTTCCA GAAATGGCAT TTAGCCCAGA TTGTCCTAAA
    GCAGTTGGAT GGGAAAAGAA CCAGAAAGGA GGCATGGGAC CAAGGATGGT GAACCTCAGT
    GAATGTATGG ACCCTAAAAG GTTAGCTGAG TCATCAGTGG ATCTAAATCT CAAACTGATG
    TGTTGGAGAT TGGTTCCTAC TTTAGACTTG GACAAGGTTG TGTCTGTCAA ATGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGCAATGTA GCTAGGACGT TGATGGGTTG GGGCGTGAGA
    CACATCACAT TTGTGGACAA TGCCAAGATC TCCTACTCCA ATCCTGTGAG GCAGCCTCTC
    TATGAGTTTG AAGATTGCCT AGGGGGTGGT AAGCCCAAGG CTCTGGCAGC AGCGGACCGG
    CTCCAGAAAA TATTCCCCGG TGTGAATGCC AGAGGATTCA ACATGAGCAT ACCTATGCCT
    GGGCATCCAG TGAACTTCTC CAGTGTCACT CTGGAGCAAG CCCGCAGAGA TGTGGAGCAA
    CTGGAGCAGC TCATCGAAAG CCATGATGTC GTCTTCCTAT TGATGGACAC CAGGGAGAGC
    CGGTGGCTTC CTGCCGTCAT TGCTGCAAGC AAGAGAAAGC TGGTCATCAA TGCTGCTTTG
    GGATTTGACA CATTTGTTGT CATGAGACAT GGTCTGAAGA AACCAAAGCA GCAAGGAGCT
    GGGGACTTGT GTCCAAACCA CCCTGTGGCA TCTGCTGACC TCCTGGGCTC ATCGCTTTTT
    GCCAACATCC CTGGTTACAA GCTTGGCTGC TACTTCTGCA ATGATGTGGT GGCCCCAGGA
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGTCGTCC AGGACTGGCC
    GTGATTGCAG GAGCCCTGGC CGTGGAATTG ATGGTATCTG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAT CGGATGAATG AGCCTCCAAC CTCTCTTGGG
    CTTGTGCCTC ACCAGATCCG GGGATTTCTT TCACGGTTTG ATAATGTCCT TCCCGTCAGC
    CTGGCATTTG ACAAATGTAC AGCTTGTTCT TCCAAAGTTC TTGATCAATA TGAACGAGAA
    GGATTTAACT TCCTAGCCAA GGTGTTTAAT TCTTCACATT CCTTCTTAGA AGACTTGACT
    GGTCTTACAT TGCTGCATCA AGAAACCCAA GCTGCTGAGA AAAAAATCCC TATGTACCAA
    TCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016861036.1
    CDS449..2554
    Translation

    Target ORF information:

    RefSeq Version XM_017005547.2
    Organism Homo sapiens(human)
    Definition Homo sapiens autophagy related 7 (ATG7), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017005547.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGCGGCAG CTACGGGGGA TCCTGGACTC TCTAAACTGC AGTTTGCCCC TTTTAGTAGT 
    GCCTTGGATG TTGGGTTTTG GCATGAGTTG ACCCAGAAGA AGCTGAACGA GTATCGGCTG
    GATGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAATG GTGACTCTGC TGGGCTGCCA
    GCTCGCTTAA CATTGGAGTT CAGTGCTTTT GACATGAGTG CTCCCACCCC AGCCCGTTGC
    TGCCCAGCTA TTGGAACACT GTATAACACC AACACACTCG AGTCTTTCAA GACTGCAGAT
    AAGAAGCTCC TTTTGGAACA AGCAGCAAAT GAGATATGGG AATCCATAAA ATCAGGCACT
    GCTCTTGAAA ACCCTGTACT CCTCAACAAG TTCCTCCTCT TGACATTTGC AGATCTAAAG
    AAGTACCACT TCTACTATTG GTTTTGCTAT CCTGCCCTCT GTCTTCCAGA GAGTTTACCT
    CTCATTCAGG GGCCAGTGGG TTTGGATCAA AGGTTTTCAC TAAAACAGAT TGAAGCACTA
    GAGTGTGCAT ATGATAATCT TTGTCAAACA GAAGGAGTCA CAGCTCTTCC TTACTTCTTA
    ATCAAGTATG ATGAGAACAT GGTGCTGGTT TCCTTGCTTA AACACTACAG TGATTTCTTC
    CAAGGTCAAA GGACGAAGAT AACAATTGGT GTATATGATC CCTGTAACTT AGCCCAGTAC
    CCTGGATGGC CTTTGAGGAA TTTTTTGGTC CTAGCAGCCC ACAGATGGAG TAGCAGTTTC
    CAGTCTGTTG AAGTTGTTTG CTTCCGTGAC CGTACCATGC AGGGGGCGAG AGACGTTGCC
    CACAGCATCA TCTTCGAAGT GAAGCTTCCA GAAATGGCAT TTAGCCCAGA TTGTCCTAAA
    GCAGTTGGAT GGGAAAAGAA CCAGAAAGGA GGCATGGGAC CAAGGATGGT GAACCTCAGT
    GAATGTATGG ACCCTAAAAG GTTAGCTGAG TCATCAGTGG ATCTAAATCT CAAACTGATG
    TGTTGGAGAT TGGTTCCTAC TTTAGACTTG GACAAGGTTG TGTCTGTCAA ATGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGCAATGTA GCTAGGACGT TGATGGGTTG GGGCGTGAGA
    CACATCACAT TTGTGGACAA TGCCAAGATC TCCTACTCCA ATCCTGTGAG GCAGCCTCTC
    TATGAGTTTG AAGATTGCCT AGGGGGTGGT AAGCCCAAGG CTCTGGCAGC AGCGGACCGG
    CTCCAGAAAA TATTCCCCGG TGTGAATGCC AGAGGATTCA ACATGAGCAT ACCTATGCCT
    GGGCATCCAG TGAACTTCTC CAGTGTCACT CTGGAGCAAG CCCGCAGAGA TGTGGAGCAA
    CTGGAGCAGC TCATCGAAAG CCATGATGTC GTCTTCCTAT TGATGGACAC CAGGGAGAGC
    CGGTGGCTTC CTGCCGTCAT TGCTGCAAGC AAGAGAAAGC TGGTCATCAA TGCTGCTTTG
    GGATTTGACA CATTTGTTGT CATGAGACAT GGTCTGAAGA AACCAAAGCA GCAAGGAGCT
    GGGGACTTGT GTCCAAACCA CCCTGTGGCA TCTGCTGACC TCCTGGGCTC ATCGCTTTTT
    GCCAACATCC CTGGTTACAA GCTTGGCTGC TACTTCTGCA ATGATGTGGT GGCCCCAGGA
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGTCGTCC AGGACTGGCC
    GTGATTGCAG GAGCCCTGGC CGTGGAATTG ATGGTATCTG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAT CGGATGAATG AGCCTCCAAC CTCTCTTGGG
    CTTGTGCCTC ACCAGATCCG GGGATTTCTT TCACGGTTTG ATAATGTCCT TCCCGTCAGC
    CTGGCATTTG ACAAATGTAC AGCTTGTTCT TCCAAAGTTC TTGATCAATA TGAACGAGAA
    GGATTTAACT TCCTAGCCAA GGTGTTTAAT TCTTCACATT CCTTCTTAGA AGACTTGACT
    GGTCTTACAT TGCTGCATCA AGAAACCCAA GCTGCTGAGA AAAAAATCCC TATGTACCAA
    TCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu93734
    Clone ID Related Accession (Same CDS sequence) XM_017005547.2 , XM_017005547.1
    Accession Version XM_017005547.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2106bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product ubiquitin-like modifier-activating enzyme ATG7 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGCGGCAG CTACGGGGGA TCCTGGACTC TCTAAACTGC AGTTTGCCCC TTTTAGTAGT 
    GCCTTGGATG TTGGGTTTTG GCATGAGTTG ACCCAGAAGA AGCTGAACGA GTATCGGCTG
    GATGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAATG GTGACTCTGC TGGGCTGCCA
    GCTCGCTTAA CATTGGAGTT CAGTGCTTTT GACATGAGTG CTCCCACCCC AGCCCGTTGC
    TGCCCAGCTA TTGGAACACT GTATAACACC AACACACTCG AGTCTTTCAA GACTGCAGAT
    AAGAAGCTCC TTTTGGAACA AGCAGCAAAT GAGATATGGG AATCCATAAA ATCAGGCACT
    GCTCTTGAAA ACCCTGTACT CCTCAACAAG TTCCTCCTCT TGACATTTGC AGATCTAAAG
    AAGTACCACT TCTACTATTG GTTTTGCTAT CCTGCCCTCT GTCTTCCAGA GAGTTTACCT
    CTCATTCAGG GGCCAGTGGG TTTGGATCAA AGGTTTTCAC TAAAACAGAT TGAAGCACTA
    GAGTGTGCAT ATGATAATCT TTGTCAAACA GAAGGAGTCA CAGCTCTTCC TTACTTCTTA
    ATCAAGTATG ATGAGAACAT GGTGCTGGTT TCCTTGCTTA AACACTACAG TGATTTCTTC
    CAAGGTCAAA GGACGAAGAT AACAATTGGT GTATATGATC CCTGTAACTT AGCCCAGTAC
    CCTGGATGGC CTTTGAGGAA TTTTTTGGTC CTAGCAGCCC ACAGATGGAG TAGCAGTTTC
    CAGTCTGTTG AAGTTGTTTG CTTCCGTGAC CGTACCATGC AGGGGGCGAG AGACGTTGCC
    CACAGCATCA TCTTCGAAGT GAAGCTTCCA GAAATGGCAT TTAGCCCAGA TTGTCCTAAA
    GCAGTTGGAT GGGAAAAGAA CCAGAAAGGA GGCATGGGAC CAAGGATGGT GAACCTCAGT
    GAATGTATGG ACCCTAAAAG GTTAGCTGAG TCATCAGTGG ATCTAAATCT CAAACTGATG
    TGTTGGAGAT TGGTTCCTAC TTTAGACTTG GACAAGGTTG TGTCTGTCAA ATGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGCAATGTA GCTAGGACGT TGATGGGTTG GGGCGTGAGA
    CACATCACAT TTGTGGACAA TGCCAAGATC TCCTACTCCA ATCCTGTGAG GCAGCCTCTC
    TATGAGTTTG AAGATTGCCT AGGGGGTGGT AAGCCCAAGG CTCTGGCAGC AGCGGACCGG
    CTCCAGAAAA TATTCCCCGG TGTGAATGCC AGAGGATTCA ACATGAGCAT ACCTATGCCT
    GGGCATCCAG TGAACTTCTC CAGTGTCACT CTGGAGCAAG CCCGCAGAGA TGTGGAGCAA
    CTGGAGCAGC TCATCGAAAG CCATGATGTC GTCTTCCTAT TGATGGACAC CAGGGAGAGC
    CGGTGGCTTC CTGCCGTCAT TGCTGCAAGC AAGAGAAAGC TGGTCATCAA TGCTGCTTTG
    GGATTTGACA CATTTGTTGT CATGAGACAT GGTCTGAAGA AACCAAAGCA GCAAGGAGCT
    GGGGACTTGT GTCCAAACCA CCCTGTGGCA TCTGCTGACC TCCTGGGCTC ATCGCTTTTT
    GCCAACATCC CTGGTTACAA GCTTGGCTGC TACTTCTGCA ATGATGTGGT GGCCCCAGGA
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGTCGTCC AGGACTGGCC
    GTGATTGCAG GAGCCCTGGC CGTGGAATTG ATGGTATCTG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAT CGGATGAATG AGCCTCCAAC CTCTCTTGGG
    CTTGTGCCTC ACCAGATCCG GGGATTTCTT TCACGGTTTG ATAATGTCCT TCCCGTCAGC
    CTGGCATTTG ACAAATGTAC AGCTTGTTCT TCCAAAGTTC TTGATCAATA TGAACGAGAA
    GGATTTAACT TCCTAGCCAA GGTGTTTAAT TCTTCACATT CCTTCTTAGA AGACTTGACT
    GGTCTTACAT TGCTGCATCA AGAAACCCAA GCTGCTGAGA AAAAAATCCC TATGTACCAA
    TCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016861036.1
    CDS449..2554
    Translation

    Target ORF information:

    RefSeq Version XM_017005547.1
    Organism Homo sapiens(human)
    Definition Homo sapiens autophagy related 7 (ATG7), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017005547.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGCGGCAG CTACGGGGGA TCCTGGACTC TCTAAACTGC AGTTTGCCCC TTTTAGTAGT 
    GCCTTGGATG TTGGGTTTTG GCATGAGTTG ACCCAGAAGA AGCTGAACGA GTATCGGCTG
    GATGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAATG GTGACTCTGC TGGGCTGCCA
    GCTCGCTTAA CATTGGAGTT CAGTGCTTTT GACATGAGTG CTCCCACCCC AGCCCGTTGC
    TGCCCAGCTA TTGGAACACT GTATAACACC AACACACTCG AGTCTTTCAA GACTGCAGAT
    AAGAAGCTCC TTTTGGAACA AGCAGCAAAT GAGATATGGG AATCCATAAA ATCAGGCACT
    GCTCTTGAAA ACCCTGTACT CCTCAACAAG TTCCTCCTCT TGACATTTGC AGATCTAAAG
    AAGTACCACT TCTACTATTG GTTTTGCTAT CCTGCCCTCT GTCTTCCAGA GAGTTTACCT
    CTCATTCAGG GGCCAGTGGG TTTGGATCAA AGGTTTTCAC TAAAACAGAT TGAAGCACTA
    GAGTGTGCAT ATGATAATCT TTGTCAAACA GAAGGAGTCA CAGCTCTTCC TTACTTCTTA
    ATCAAGTATG ATGAGAACAT GGTGCTGGTT TCCTTGCTTA AACACTACAG TGATTTCTTC
    CAAGGTCAAA GGACGAAGAT AACAATTGGT GTATATGATC CCTGTAACTT AGCCCAGTAC
    CCTGGATGGC CTTTGAGGAA TTTTTTGGTC CTAGCAGCCC ACAGATGGAG TAGCAGTTTC
    CAGTCTGTTG AAGTTGTTTG CTTCCGTGAC CGTACCATGC AGGGGGCGAG AGACGTTGCC
    CACAGCATCA TCTTCGAAGT GAAGCTTCCA GAAATGGCAT TTAGCCCAGA TTGTCCTAAA
    GCAGTTGGAT GGGAAAAGAA CCAGAAAGGA GGCATGGGAC CAAGGATGGT GAACCTCAGT
    GAATGTATGG ACCCTAAAAG GTTAGCTGAG TCATCAGTGG ATCTAAATCT CAAACTGATG
    TGTTGGAGAT TGGTTCCTAC TTTAGACTTG GACAAGGTTG TGTCTGTCAA ATGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGCAATGTA GCTAGGACGT TGATGGGTTG GGGCGTGAGA
    CACATCACAT TTGTGGACAA TGCCAAGATC TCCTACTCCA ATCCTGTGAG GCAGCCTCTC
    TATGAGTTTG AAGATTGCCT AGGGGGTGGT AAGCCCAAGG CTCTGGCAGC AGCGGACCGG
    CTCCAGAAAA TATTCCCCGG TGTGAATGCC AGAGGATTCA ACATGAGCAT ACCTATGCCT
    GGGCATCCAG TGAACTTCTC CAGTGTCACT CTGGAGCAAG CCCGCAGAGA TGTGGAGCAA
    CTGGAGCAGC TCATCGAAAG CCATGATGTC GTCTTCCTAT TGATGGACAC CAGGGAGAGC
    CGGTGGCTTC CTGCCGTCAT TGCTGCAAGC AAGAGAAAGC TGGTCATCAA TGCTGCTTTG
    GGATTTGACA CATTTGTTGT CATGAGACAT GGTCTGAAGA AACCAAAGCA GCAAGGAGCT
    GGGGACTTGT GTCCAAACCA CCCTGTGGCA TCTGCTGACC TCCTGGGCTC ATCGCTTTTT
    GCCAACATCC CTGGTTACAA GCTTGGCTGC TACTTCTGCA ATGATGTGGT GGCCCCAGGA
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGTCGTCC AGGACTGGCC
    GTGATTGCAG GAGCCCTGGC CGTGGAATTG ATGGTATCTG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAT CGGATGAATG AGCCTCCAAC CTCTCTTGGG
    CTTGTGCCTC ACCAGATCCG GGGATTTCTT TCACGGTTTG ATAATGTCCT TCCCGTCAGC
    CTGGCATTTG ACAAATGTAC AGCTTGTTCT TCCAAAGTTC TTGATCAATA TGAACGAGAA
    GGATTTAACT TCCTAGCCAA GGTGTTTAAT TCTTCACATT CCTTCTTAGA AGACTTGACT
    GGTCTTACAT TGCTGCATCA AGAAACCCAA GCTGCTGAGA AAAAAATCCC TATGTACCAA
    TCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu93735
    Clone ID Related Accession (Same CDS sequence) XM_017005550.1
    Accession Version XM_017005550.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2037bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product ubiquitin-like modifier-activating enzyme ATG7 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGCGGCAG CTACGGGGGA TCCTGGACTC TCTAAACTGC AGTTTGCCCC TTTTAGTAGT 
    GCCTTGGATG TTGGGTTTTG GCATGAGTTG ACCCAGAAGA AGCTGAACGA GTATCGGCTG
    GATGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAATG GTGACTCTGC TGGGCTGCCA
    GCTCGCTTAA CATTGGAGTT CAGTGCTTTT GACATGAGTG CTCCCACCCC AGCCCGTTGC
    TGCCCAGCTA TTGGAACACT GTATAACACC AACACACTCG AGTCTTTCAA GACTGCAGAT
    AAGAAGCTCC TTTTGGAACA AGCAGCAAAT GAGATATGGG AATCCATAAA ATCAGGCACT
    GCTCTTGAAA ACCCTGTACT CCTCAACAAG TTCCTCCTCT TGACATTTGC AGATCTAAAG
    AAGTACCACT TCTACTATTG GTTTTGCTAT CCTGCCCTCT GTCTTCCAGA GAGTTTACCT
    CTCATTCAGG GGCCAGTGGG TTTGGATCAA AGGTTTTCAC TAAAACAGAT TGAAGCACTA
    GAGTGTGCAT ATGATAATCT TTGTCAAACA GAAGGAGTCA CAGCTCTTCC TTACTTCTTA
    ATCAAGTATG ATGAGAACAT GGTGCTGGTT TCCTTGCTTA AACACTACAG TGATTTCTTC
    CAAGGTCAAA GGACGAAGAT AACAATTGGT GTATATGATC CCTGTAACTT AGCCCAGTAC
    CCTGGATGGC CTTTGAGGAA TTTTTTGGTC CTAGCAGCCC ACAGATGGAG TAGCAGTTTC
    CAGTCTGTTG AAGTTGTTTG CTTCCGTGAC CGTACCATGC AGGGGGCGAG AGACGTTGCC
    CACAGCATCA TCTTCGAAGT GAAGCTTCCA GAAATGGCAT TTAGCCCAGA TTGTCCTAAA
    GCAGTTGGAT GGGAAAAGAA CCAGAAAGGA GGCATGGGAC CAAGGATGGT GAACCTCAGT
    GAATGTATGG ACCCTAAAAG GTTAGCTGAG TCATCAGTGG ATCTAAATCT CAAACTGATG
    TGTTGGAGAT TGGTTCCTAC TTTAGACTTG GACAAGGTTG TGTCTGTCAA ATGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGCAATGTA GCTAGGACGT TGATGAATGC CAGAGGATTC
    AACATGAGCA TACCTATGCC TGGGCATCCA GTGAACTTCT CCAGTGTCAC TCTGGAGCAA
    GCCCGCAGAG ATGTGGAGCA ACTGGAGCAG CTCATCGAAA GCCATGATGT CGTCTTCCTA
    TTGATGGACA CCAGGGAGAG CCGGTGGCTT CCTGCCGTCA TTGCTGCAAG CAAGAGAAAG
    CTGGTCATCA ATGCTGCTTT GGGATTTGAC ACATTTGTTG TCATGAGACA TGGTCTGAAG
    AAACCAAAGC AGCAAGGAGC TGGGGACTTG TGTCCAAACC ACCCTGTGGC ATCTGCTGAC
    CTCCTGGGCT CATCGCTTTT TGCCAACATC CCTGGTTACA AGCTTGGCTG CTACTTCTGC
    AATGATGTGG TGGCCCCAGG AGATTCAACC AGAGACCGGA CCTTGGACCA GCAGTGCACT
    GTGAGTCGTC CAGGACTGGC CGTGATTGCA GGAGCCCTGG CCGTGGAATT GATGGTATCT
    GTTTTGCAGC ATCCAGAAGG GGGCTATGCC ATTGCCAGCA GCAGTGACGA TCGGATGAAT
    GAGCCTCCAA CCTCTCTTGG GCTTGTGCCT CACCAGATCC GGGGATTTCT TTCACGGTTT
    GATAATGTCC TTCCCGTCAG CCTGGCATTT GACAAATGTA CAGCTTGTTC TTCCAAAGTT
    CTTGATCAAT ATGAACGAGA AGGATTTAAC TTCCTAGCCA AGGTGTTTAA TTCTTCACAT
    TCCTTCTTAG AAGACTTGAC TGGTCTTACA TTGCTGCATC AAGAAACCCA AGCTGCTGAG
    CTTCATCAAG AGCCTCTGAT GACAAATATG CCTTTGGAGG CCAGCAGAAG CTGTGGGAAA
    CCCCCGTGTC AGCACTCAGA CCTGTTTCCA ATGGAGAGAA ATCGCTCTGG GGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016861039.1
    CDS61..2097
    Translation

    Target ORF information:

    RefSeq Version XM_017005550.1
    Organism Homo sapiens(human)
    Definition Homo sapiens autophagy related 7 (ATG7), transcript variant X20, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017005550.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGCGGCAG CTACGGGGGA TCCTGGACTC TCTAAACTGC AGTTTGCCCC TTTTAGTAGT 
    GCCTTGGATG TTGGGTTTTG GCATGAGTTG ACCCAGAAGA AGCTGAACGA GTATCGGCTG
    GATGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAATG GTGACTCTGC TGGGCTGCCA
    GCTCGCTTAA CATTGGAGTT CAGTGCTTTT GACATGAGTG CTCCCACCCC AGCCCGTTGC
    TGCCCAGCTA TTGGAACACT GTATAACACC AACACACTCG AGTCTTTCAA GACTGCAGAT
    AAGAAGCTCC TTTTGGAACA AGCAGCAAAT GAGATATGGG AATCCATAAA ATCAGGCACT
    GCTCTTGAAA ACCCTGTACT CCTCAACAAG TTCCTCCTCT TGACATTTGC AGATCTAAAG
    AAGTACCACT TCTACTATTG GTTTTGCTAT CCTGCCCTCT GTCTTCCAGA GAGTTTACCT
    CTCATTCAGG GGCCAGTGGG TTTGGATCAA AGGTTTTCAC TAAAACAGAT TGAAGCACTA
    GAGTGTGCAT ATGATAATCT TTGTCAAACA GAAGGAGTCA CAGCTCTTCC TTACTTCTTA
    ATCAAGTATG ATGAGAACAT GGTGCTGGTT TCCTTGCTTA AACACTACAG TGATTTCTTC
    CAAGGTCAAA GGACGAAGAT AACAATTGGT GTATATGATC CCTGTAACTT AGCCCAGTAC
    CCTGGATGGC CTTTGAGGAA TTTTTTGGTC CTAGCAGCCC ACAGATGGAG TAGCAGTTTC
    CAGTCTGTTG AAGTTGTTTG CTTCCGTGAC CGTACCATGC AGGGGGCGAG AGACGTTGCC
    CACAGCATCA TCTTCGAAGT GAAGCTTCCA GAAATGGCAT TTAGCCCAGA TTGTCCTAAA
    GCAGTTGGAT GGGAAAAGAA CCAGAAAGGA GGCATGGGAC CAAGGATGGT GAACCTCAGT
    GAATGTATGG ACCCTAAAAG GTTAGCTGAG TCATCAGTGG ATCTAAATCT CAAACTGATG
    TGTTGGAGAT TGGTTCCTAC TTTAGACTTG GACAAGGTTG TGTCTGTCAA ATGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGCAATGTA GCTAGGACGT TGATGAATGC CAGAGGATTC
    AACATGAGCA TACCTATGCC TGGGCATCCA GTGAACTTCT CCAGTGTCAC TCTGGAGCAA
    GCCCGCAGAG ATGTGGAGCA ACTGGAGCAG CTCATCGAAA GCCATGATGT CGTCTTCCTA
    TTGATGGACA CCAGGGAGAG CCGGTGGCTT CCTGCCGTCA TTGCTGCAAG CAAGAGAAAG
    CTGGTCATCA ATGCTGCTTT GGGATTTGAC ACATTTGTTG TCATGAGACA TGGTCTGAAG
    AAACCAAAGC AGCAAGGAGC TGGGGACTTG TGTCCAAACC ACCCTGTGGC ATCTGCTGAC
    CTCCTGGGCT CATCGCTTTT TGCCAACATC CCTGGTTACA AGCTTGGCTG CTACTTCTGC
    AATGATGTGG TGGCCCCAGG AGATTCAACC AGAGACCGGA CCTTGGACCA GCAGTGCACT
    GTGAGTCGTC CAGGACTGGC CGTGATTGCA GGAGCCCTGG CCGTGGAATT GATGGTATCT
    GTTTTGCAGC ATCCAGAAGG GGGCTATGCC ATTGCCAGCA GCAGTGACGA TCGGATGAAT
    GAGCCTCCAA CCTCTCTTGG GCTTGTGCCT CACCAGATCC GGGGATTTCT TTCACGGTTT
    GATAATGTCC TTCCCGTCAG CCTGGCATTT GACAAATGTA CAGCTTGTTC TTCCAAAGTT
    CTTGATCAAT ATGAACGAGA AGGATTTAAC TTCCTAGCCA AGGTGTTTAA TTCTTCACAT
    TCCTTCTTAG AAGACTTGAC TGGTCTTACA TTGCTGCATC AAGAAACCCA AGCTGCTGAG
    CTTCATCAAG AGCCTCTGAT GACAAATATG CCTTTGGAGG CCAGCAGAAG CTGTGGGAAA
    CCCCCGTGTC AGCACTCAGA CCTGTTTCCA ATGGAGAGAA ATCGCTCTGG GGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu93736
    Clone ID Related Accession (Same CDS sequence) XM_017005551.1
    Accession Version XM_017005551.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2019bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product ubiquitin-like modifier-activating enzyme ATG7 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGCGGCAG CTACGGGGGA TCCTGGACTC TCTAAACTGC AGTTTGCCCC TTTTAGTAGT 
    GCCTTGGATG TTGGGTTTTG GCATGAGTTG ACCCAGAAGA AGCTGAACGA GTATCGGCTG
    GATGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAATG GTGACTCTGC TGGGCTGCCA
    GCTCGCTTAA CATTGGAGTT CAGTGCTTTT GACATGAGTG CTCCCACCCC AGCCCGTTGC
    TGCCCAGCTA TTGGAACACT GTATAACACC AACACACTCG AGTCTTTCAA GACTGCAGAT
    AAGAAGCTCC TTTTGGAACA AGCAGCAAAT GAGATATGGG AATCCATAAA ATCAGGCACT
    GCTCTTGAAA ACCCTGTACT CCTCAACAAG TTCCTCCTCT TGACATTTGC AGATCTAAAG
    AAGTACCACT TCTACTATTG GTTTTGCTAT CCTGCCCTCT GTCTTCCAGA GAGTTTACCT
    CTCATTCAGG GGCCAGTGGG TTTGGATCAA AGGTTTTCAC TAAAACAGAT TGAAGCACTA
    GAGTGTGCAT ATGATAATCT TTGTCAAACA GAAGGAGTCA CAGCTCTTCC TTACTTCTTA
    ATCAAGTATG ATGAGAACAT GGTGCTGGTT TCCTTGCTTA AACACTACAG TGATTTCTTC
    CAAGGTCAAA GGACGAAGAT AACAATTGGT GTATATGATC CCTGTAACTT AGCCCAGTAC
    CCTGGATGGC CTTTGAGGAA TTTTTTGGTC CTAGCAGCCC ACAGATGGAG TAGCAGTTTC
    CAGTCTGTTG AAGTTGTTTG CTTCCGTGAC CGTACCATGC AGGGGGCGAG AGACGTTGCC
    CACAGCATCA TCTTCGAAGT GAAGCTTCCA GAAATGGCAT TTAGCCCAGA TTGTCCTAAA
    GCAGTTGGAT GGGAAAAGAA CCAGAAAGGA GGCATGGGAC CAAGGATGGT GAACCTCAGT
    GAATGTATGG ACCCTAAAAG GTTAGCTGAG TCATCAGTGG ATCTAAATCT CAAACTGATG
    TGTTGGAGAT TGGTTCCTAC TTTAGACTTG GACAAGGTTG TGTCTGTCAA ATGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGCAATGTA GCTAGGACGT TGATGGGTTG GGGCGTGAGA
    CACATCACAT TTGTGGACAA TGCCAAGATC TCCTACTCCA ATCCTGTGAG GCAGCCTCTC
    TATGAGTTTG AAGATTGCCT AGGGGGTGGT AAGCCCAAGG CTCTGGCAGC AGCGGACCGG
    CTCCAGAAAA TATTCCCCGG TGTGAATGCC AGAGGATTCA ACATGAGCAT ACCTATGCCT
    GGGCATCCAG TGAACTTCTC CAGTGTCACT CTGGAGCAAG CCCGCAGAGA TGTGGAGCAA
    CTGGAGCAGC TCATCGAAAG CCATGATGTC GTCTTCCTAT TGATGGACAC CAGGGAGAGC
    CGGTGGCTTC CTGCCGTCAT TGCTGCAAGC AAGAGAAAGC TGGTCATCAA TGCTGCTTTG
    GGATTTGACA CATTTGTTGT CATGAGACAT GGTCTGAAGA AACCAAAGCA GCAAGGAGCT
    GGGGACTTGT GTCCAAACCA CCCTGTGGCA TCTGCTGACC TCCTGGGCTC ATCGCTTTTT
    GCCAACATCC CTGGTTACAA GCTTGGCTGC TACTTCTGCA ATGATGTGGT GGCCCCAGGA
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGTCGTCC AGGACTGGCC
    GTGATTGCAG GAGCCCTGGC CGTGGAATTG ATGGTATCTG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAT CGGATGAATG AGCCTCCAAC CTCTCTTGGG
    CTTGTGCCTC ACCAGGTTCT TGATCAATAT GAACGAGAAG GATTTAACTT CCTAGCCAAG
    GTGTTTAATT CTTCACATTC CTTCTTAGAA GACTTGACTG GTCTTACATT GCTGCATCAA
    GAAACCCAAG CTGCTGAGGC AGAATATGAA ATTCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016861040.1
    CDS61..2079
    Translation

    Target ORF information:

    RefSeq Version XM_017005551.1
    Organism Homo sapiens(human)
    Definition Homo sapiens autophagy related 7 (ATG7), transcript variant X22, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017005551.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGCGGCAG CTACGGGGGA TCCTGGACTC TCTAAACTGC AGTTTGCCCC TTTTAGTAGT 
    GCCTTGGATG TTGGGTTTTG GCATGAGTTG ACCCAGAAGA AGCTGAACGA GTATCGGCTG
    GATGAAGCTC CCAAGGACAT TAAGGGTTAT TACTACAATG GTGACTCTGC TGGGCTGCCA
    GCTCGCTTAA CATTGGAGTT CAGTGCTTTT GACATGAGTG CTCCCACCCC AGCCCGTTGC
    TGCCCAGCTA TTGGAACACT GTATAACACC AACACACTCG AGTCTTTCAA GACTGCAGAT
    AAGAAGCTCC TTTTGGAACA AGCAGCAAAT GAGATATGGG AATCCATAAA ATCAGGCACT
    GCTCTTGAAA ACCCTGTACT CCTCAACAAG TTCCTCCTCT TGACATTTGC AGATCTAAAG
    AAGTACCACT TCTACTATTG GTTTTGCTAT CCTGCCCTCT GTCTTCCAGA GAGTTTACCT
    CTCATTCAGG GGCCAGTGGG TTTGGATCAA AGGTTTTCAC TAAAACAGAT TGAAGCACTA
    GAGTGTGCAT ATGATAATCT TTGTCAAACA GAAGGAGTCA CAGCTCTTCC TTACTTCTTA
    ATCAAGTATG ATGAGAACAT GGTGCTGGTT TCCTTGCTTA AACACTACAG TGATTTCTTC
    CAAGGTCAAA GGACGAAGAT AACAATTGGT GTATATGATC CCTGTAACTT AGCCCAGTAC
    CCTGGATGGC CTTTGAGGAA TTTTTTGGTC CTAGCAGCCC ACAGATGGAG TAGCAGTTTC
    CAGTCTGTTG AAGTTGTTTG CTTCCGTGAC CGTACCATGC AGGGGGCGAG AGACGTTGCC
    CACAGCATCA TCTTCGAAGT GAAGCTTCCA GAAATGGCAT TTAGCCCAGA TTGTCCTAAA
    GCAGTTGGAT GGGAAAAGAA CCAGAAAGGA GGCATGGGAC CAAGGATGGT GAACCTCAGT
    GAATGTATGG ACCCTAAAAG GTTAGCTGAG TCATCAGTGG ATCTAAATCT CAAACTGATG
    TGTTGGAGAT TGGTTCCTAC TTTAGACTTG GACAAGGTTG TGTCTGTCAA ATGTCTGCTG
    CTTGGAGCCG GCACCTTGGG TTGCAATGTA GCTAGGACGT TGATGGGTTG GGGCGTGAGA
    CACATCACAT TTGTGGACAA TGCCAAGATC TCCTACTCCA ATCCTGTGAG GCAGCCTCTC
    TATGAGTTTG AAGATTGCCT AGGGGGTGGT AAGCCCAAGG CTCTGGCAGC AGCGGACCGG
    CTCCAGAAAA TATTCCCCGG TGTGAATGCC AGAGGATTCA ACATGAGCAT ACCTATGCCT
    GGGCATCCAG TGAACTTCTC CAGTGTCACT CTGGAGCAAG CCCGCAGAGA TGTGGAGCAA
    CTGGAGCAGC TCATCGAAAG CCATGATGTC GTCTTCCTAT TGATGGACAC CAGGGAGAGC
    CGGTGGCTTC CTGCCGTCAT TGCTGCAAGC AAGAGAAAGC TGGTCATCAA TGCTGCTTTG
    GGATTTGACA CATTTGTTGT CATGAGACAT GGTCTGAAGA AACCAAAGCA GCAAGGAGCT
    GGGGACTTGT GTCCAAACCA CCCTGTGGCA TCTGCTGACC TCCTGGGCTC ATCGCTTTTT
    GCCAACATCC CTGGTTACAA GCTTGGCTGC TACTTCTGCA ATGATGTGGT GGCCCCAGGA
    GATTCAACCA GAGACCGGAC CTTGGACCAG CAGTGCACTG TGAGTCGTCC AGGACTGGCC
    GTGATTGCAG GAGCCCTGGC CGTGGAATTG ATGGTATCTG TTTTGCAGCA TCCAGAAGGG
    GGCTATGCCA TTGCCAGCAG CAGTGACGAT CGGATGAATG AGCCTCCAAC CTCTCTTGGG
    CTTGTGCCTC ACCAGGTTCT TGATCAATAT GAACGAGAAG GATTTAACTT CCTAGCCAAG
    GTGTTTAATT CTTCACATTC CTTCTTAGAA GACTTGACTG GTCTTACATT GCTGCATCAA
    GAAACCCAAG CTGCTGAGGC AGAATATGAA ATTCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.