VAT1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	VAT1
Entrez Gene ID	10493
Full Name	vesicle amine transport 1
Synonyms	VATI
General protein information	Preferred Names vesicle amine transport 1 Names synaptic vesicle membrane protein VAT-1 homolog membrane protein of cholinergic synaptic vesicles vesicle amine transport protein 1 homolog (T. californica)
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 17 Map Location: 17q21.31
Summary	Synaptic vesicles are responsible for regulating the storage and release of neurotransmitters in the nerve terminal. The protein encoded by this gene is an abundant integral membrane protein of cholinergic synaptic vesicles and is thought to be involved in vesicular transport. It belongs to the quinone oxidoreductase subfamily of zinc-containing alcohol dehydrogenase proteins. [provided by RefSeq, Jul 2008].
Disorder MIM:	604631

mRNA and Protein(s)

mRNA	Protein	Name
NM_006373.3	NP_006364.2	synaptic vesicle membrane protein VAT-1 homolog
NM_006373.4	NP_006364.2	synaptic vesicle membrane protein VAT-1 homolog

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	VAT1	XP_001158563.1
Canis lupus familiaris (dog)	VAT1	XP_548076.1
Homo sapiens (human)	VAT1	NP_006364.2
	VAT1	XP_002800538.1
Bos taurus (cattle)	VAT1	NP_001179194.1
Mus musculus (house mouse)	Vat1	NP_036167.1
Rattus norvegicus (Norway rat)	Vat1	NP_001028855.1
Xenopus tropicalis (tropical clawed frog)	vat1	XP_002932557.1
Gallus gallus (chicken)	VAT1	XP_418130.2
Danio rerio (zebrafish)	vat1	NP_001007052.3

Gene Ontology
Bibliography

Related articles in PubMed

VAT-1 is a novel pathogenic factor of progressive benign prostatic hyperplasia.
Mori F, Tanigawa K, Endo K, Minamiguchi K, Abe M, Yamada S, Miyoshi K
The Prostate71(14)1579-86(2011 Oct)

An essential role for phospholipase D in the recruitment of vesicle amine transport protein-1 to membranes in human neutrophils.
Faugaret D, Chouinard FC, Harbour D, El azreq MA, Bourgoin SG
Biochemical pharmacology81(1)144-56(2011 Jan)

Vesicle amine transport protein-1 (VAT-1) is upregulated in glioblastomas and promotes migration.
Mertsch S, Becker M, Lichota A, Paulus W, Senner V
Neuropathology and applied neurobiology35(4)342-52(2009 Aug)

Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR
Journal of proteome research8(3)1304-14(2009 Mar)

Large-scale proteomics and phosphoproteomics of urinary exosomes.
Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal of the American Society of Nephrology : JASN20(2)363-79(2009 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

We demonstrate that VAT-1 is a novel pathogenic factor in benign prostatic hyperplasia associated with cell proliferation.
Title: VAT-1 is a novel pathogenic factor of progressive benign prostatic hyperplasia.

The role of phospholipase D in the transport of VAT1 to plasma membranes and in phosphatidic acid metabolism in neutrophils is reported.
Title: An essential role for phospholipase D in the recruitment of vesicle amine transport protein-1 to membranes in human neutrophils.

VAT-1 is overexpressed in glioblastomas and functionally involved in glioma cell migration, representing a new component involved in glioma invasion
Title: Vesicle amine transport protein-1 (VAT-1) is upregulated in glioblastomas and promotes migration.

The following VAT1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the VAT1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu02080	NM_006373.4 Latest version!	Homo sapiens vesicle amine transport 1 (VAT1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
View Old Accession Versions >>
OHu02080	NM_006373.3	Homo sapiens vesicle amine transport 1 (VAT1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

VAT1 ( NM_006373.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_006364.2 Homo sapiens vesicle amine transport 1 (VAT1), mRNA.

Price & Availability↑

CloneID	OHu02080
Clone ID Related Accession (Same CDS sequence)	NM_006373.3 , NM_006373.4
Accession Version	NM_006373.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1182bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	synaptic vesicle membrane protein VAT-1 homolog
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC135721.4 and AC055866.19. On or before Mar 29, 2008 this sequence version replaced XR_017880.2, NM_006373.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC015041.2, SRR1803612.81895.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGTCCGACG AGAGAGAGGT AGCCGAGGCA GCGACCGGGG AAGACGCCTC TTCGCCGCCT 
CCGAAAACCG AGGCAGCGAG CGACCCCCAG CATCCCGCGG CCTCCGAAGG GGCCGCCGCC 
GCCGCCGCCT CGCCGCCACT GCTGCGCTGC CTAGTGCTCA CCGGCTTTGG AGGCTACGAC 
AAGGTGAAGC TGCAGAGCCG GCCGGCAGCG CCCCCGGCCC CTGGGCCCGG CCAGCTGACG 
CTGCGTCTGC GGGCCTGCGG GCTCAACTTC GCAGACCTCA TGGCTAGGCA GGGGCTGTAC 
GACCGTCTCC CGCCTCTGCC TGTCACTCCG GGCATGGAGG GCGCGGGTGT TGTGATCGCA 
GTGGGCGAGG GAGTCAGCGA CCGCAAGGCA GGAGACCGGG TGATGGTGTT GAACCGGTCA 
GGGATGTGGC AGGAAGAGGT GACTGTGCCC TCGGTCCAGA CCTTCCTGAT TCCTGAGGCC 
ATGACCTTTG AGGAAGCTGC TGCCTTGCTC GTCAATTACA TTACAGCCTA CATGGTCCTC 
TTTGACTTCG GCAACCTACA GCCTGGCCAC AGCGTCTTGG TACACATGGC TGCAGGGGGT 
GTGGGTATGG CTGCCGTGCA GCTGTGCCGT ACAGTGGAGA ATGTGACAGT GTTCGGAACG 
GCCTCGGCCA GCAAGCACGA GGCACTGAAG GAGAATGGGG TCACACATCC CATCGACTAT 
CACACGACTG ACTACGTGGA TGAGATCAAG AAGATTTCCC CTAAAGGAGT GGACATTGTC 
ATGGACCCTC TGGGTGGGTC AGATACTGCC AAGGGCTACA ACCTCCTGAA ACCCATGGGC 
AAAGTCGTCA CCTATGGAAT GGCCAACCTG CTGACGGGCC CCAAACGGAA CCTGATGGCC 
CTGGCCCGGA CATGGTGGAA TCAGTTCAGC GTGACAGCTC TGCAGCTGCT GCAGGCCAAC 
CGGGCTGTGT GTGGCTTCCA CCTGGGCTAC CTGGATGGTG AGGTGGAGCT GGTCAGTGGT 
GTGGTGGCCC GCCTCCTGGC TCTGTACAAC CAGGGCCACA TCAAGCCCCA CATTGACTCA 
GTCTGGCCCT TCGAGAAGGT GGCTGATGCC ATGAAACAGA TGCAGGAGAA GAAGAATGTG 
GGCAAGGTCC TCCTGGTTCC AGGGCCAGAG AAGGAGAACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_006364.2
CDS	121..1302
Translation	MSDEREVAEAATGEDASSPPPKTEAASDPQHPAASEGAAAAAASPPLLRCLVLTGFGGYDKVKLQSRPAAPPAPGPGQLTLRLRACGLNFADLMARQGLYDRLPPLPVTPGMEGAGVVIAVGEGVSDRKAGDRVMVLNRSGMWQEEVTVPSVQTFLIPEAMTFEEAAALLVNYITAYMVLFDFGNLQPGHSVLVHMAAGGVGMAAVQLCRTVENVTVFGTASASKHEALKENGVTHPIDYHTTDYVDEIKKISPKGVDIVMDPLGGSDTAKGYNLLKPMGKVVTYGMANLLTGPKRNLMALARTWWNQFSVTALQLLQANRAVCGFHLGYLDGEVELVSGVVARLLALYNQGHIKPHIDSVWPFEKVADAMKQMQEKKNVGKVLLVPGPEKEN

Target ORF information:

RefSeq Version	NM_006373.3
Organism	Homo sapiens(human)
Definition	Homo sapiens vesicle amine transport 1 (VAT1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006373.3

ORF Insert Sequence:

ATGTCCGACG AGAGAGAGGT AGCCGAGGCA GCGACCGGGG AAGACGCCTC TTCGCCGCCT 
CCGAAAACCG AGGCAGCGAG CGACCCCCAG CATCCCGCGG CCTCCGAAGG GGCCGCCGCC 
GCCGCCGCCT CGCCGCCACT GCTGCGCTGC CTAGTGCTCA CCGGCTTTGG AGGCTACGAC 
AAGGTGAAGC TGCAGAGCCG GCCGGCAGCG CCCCCGGCCC CTGGGCCCGG CCAGCTGACG 
CTGCGTCTGC GGGCCTGCGG GCTCAACTTC GCAGACCTCA TGGCTAGGCA GGGGCTGTAC 
GACCGTCTCC CGCCTCTGCC TGTCACTCCG GGCATGGAGG GCGCGGGTGT TGTGATCGCA 
GTGGGCGAGG GAGTCAGCGA CCGCAAGGCA GGAGACCGGG TGATGGTGTT GAACCGGTCA 
GGGATGTGGC AGGAAGAGGT GACTGTGCCC TCGGTCCAGA CCTTCCTGAT TCCTGAGGCC 
ATGACCTTTG AGGAAGCTGC TGCCTTGCTC GTCAATTACA TTACAGCCTA CATGGTCCTC 
TTTGACTTCG GCAACCTACA GCCTGGCCAC AGCGTCTTGG TACACATGGC TGCAGGGGGT 
GTGGGTATGG CTGCCGTGCA GCTGTGCCGT ACAGTGGAGA ATGTGACAGT GTTCGGAACG 
GCCTCGGCCA GCAAGCACGA GGCACTGAAG GAGAATGGGG TCACACATCC CATCGACTAT 
CACACGACTG ACTACGTGGA TGAGATCAAG AAGATTTCCC CTAAAGGAGT GGACATTGTC 
ATGGACCCTC TGGGTGGGTC AGATACTGCC AAGGGCTACA ACCTCCTGAA ACCCATGGGC 
AAAGTCGTCA CCTATGGAAT GGCCAACCTG CTGACGGGCC CCAAACGGAA CCTGATGGCC 
CTGGCCCGGA CATGGTGGAA TCAGTTCAGC GTGACAGCTC TGCAGCTGCT GCAGGCCAAC 
CGGGCTGTGT GTGGCTTCCA CCTGGGCTAC CTGGATGGTG AGGTGGAGCT GGTCAGTGGT 
GTGGTGGCCC GCCTCCTGGC TCTGTACAAC CAGGGCCACA TCAAGCCCCA CATTGACTCA 
GTCTGGCCCT TCGAGAAGGT GGCTGATGCC ATGAAACAGA TGCAGGAGAA GAAGAATGTG 
GGCAAGGTCC TCCTGGTTCC AGGGCCAGAG AAGGAGAACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VAT1 ( NM_006373.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_006364.2 Homo sapiens vesicle amine transport 1 (VAT1), mRNA.

Price & Availability↑

CloneID	OHu02080
Clone ID Related Accession (Same CDS sequence)	NM_006373.3 , NM_006373.4
Accession Version	NM_006373.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1182bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-06
Organism	Homo sapiens(human)
Product	synaptic vesicle membrane protein VAT-1 homolog
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC135721.4 and AC055866.19. On Nov 22, 2018 this sequence version replaced NM_006373.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC090858.1, BC015041.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000355653.8/ ENSP00000347872.2 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTCCGACG AGAGAGAGGT AGCCGAGGCA GCGACCGGGG AAGACGCCTC TTCGCCGCCT 
CCGAAAACCG AGGCAGCGAG CGACCCCCAG CATCCCGCGG CCTCCGAAGG GGCCGCCGCC 
GCCGCCGCCT CGCCGCCACT GCTGCGCTGC CTAGTGCTCA CCGGCTTTGG AGGCTACGAC 
AAGGTGAAGC TGCAGAGCCG GCCGGCAGCG CCCCCGGCCC CTGGGCCCGG CCAGCTGACG 
CTGCGTCTGC GGGCCTGCGG GCTCAACTTC GCAGACCTCA TGGCTAGGCA GGGGCTGTAC 
GACCGTCTCC CGCCTCTGCC TGTCACTCCG GGCATGGAGG GCGCGGGTGT TGTGATCGCA 
GTGGGCGAGG GAGTCAGCGA CCGCAAGGCA GGAGACCGGG TGATGGTGTT GAACCGGTCA 
GGGATGTGGC AGGAAGAGGT GACTGTGCCC TCGGTCCAGA CCTTCCTGAT TCCTGAGGCC 
ATGACCTTTG AGGAAGCTGC TGCCTTGCTC GTCAATTACA TTACAGCCTA CATGGTCCTC 
TTTGACTTCG GCAACCTACA GCCTGGCCAC AGCGTCTTGG TACACATGGC TGCAGGGGGT 
GTGGGTATGG CTGCCGTGCA GCTGTGCCGT ACAGTGGAGA ATGTGACAGT GTTCGGAACG 
GCCTCGGCCA GCAAGCACGA GGCACTGAAG GAGAATGGGG TCACACATCC CATCGACTAT 
CACACGACTG ACTACGTGGA TGAGATCAAG AAGATTTCCC CTAAAGGAGT GGACATTGTC 
ATGGACCCTC TGGGTGGGTC AGATACTGCC AAGGGCTACA ACCTCCTGAA ACCCATGGGC 
AAAGTCGTCA CCTATGGAAT GGCCAACCTG CTGACGGGCC CCAAACGGAA CCTGATGGCC 
CTGGCCCGGA CATGGTGGAA TCAGTTCAGC GTGACAGCTC TGCAGCTGCT GCAGGCCAAC 
CGGGCTGTGT GTGGCTTCCA CCTGGGCTAC CTGGATGGTG AGGTGGAGCT GGTCAGTGGT 
GTGGTGGCCC GCCTCCTGGC TCTGTACAAC CAGGGCCACA TCAAGCCCCA CATTGACTCA 
GTCTGGCCCT TCGAGAAGGT GGCTGATGCC ATGAAACAGA TGCAGGAGAA GAAGAATGTG 
GGCAAGGTCC TCCTGGTTCC AGGGCCAGAG AAGGAGAACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_006364.2
CDS	64..1245
Translation	MSDEREVAEAATGEDASSPPPKTEAASDPQHPAASEGAAAAAASPPLLRCLVLTGFGGYDKVKLQSRPAAPPAPGPGQLTLRLRACGLNFADLMARQGLYDRLPPLPVTPGMEGAGVVIAVGEGVSDRKAGDRVMVLNRSGMWQEEVTVPSVQTFLIPEAMTFEEAAALLVNYITAYMVLFDFGNLQPGHSVLVHMAAGGVGMAAVQLCRTVENVTVFGTASASKHEALKENGVTHPIDYHTTDYVDEIKKISPKGVDIVMDPLGGSDTAKGYNLLKPMGKVVTYGMANLLTGPKRNLMALARTWWNQFSVTALQLLQANRAVCGFHLGYLDGEVELVSGVVARLLALYNQGHIKPHIDSVWPFEKVADAMKQMQEKKNVGKVLLVPGPEKEN

Target ORF information:

RefSeq Version	NM_006373.4
Organism	Homo sapiens(human)
Definition	Homo sapiens vesicle amine transport 1 (VAT1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006373.4

ORF Insert Sequence:

ATGTCCGACG AGAGAGAGGT AGCCGAGGCA GCGACCGGGG AAGACGCCTC TTCGCCGCCT 
CCGAAAACCG AGGCAGCGAG CGACCCCCAG CATCCCGCGG CCTCCGAAGG GGCCGCCGCC 
GCCGCCGCCT CGCCGCCACT GCTGCGCTGC CTAGTGCTCA CCGGCTTTGG AGGCTACGAC 
AAGGTGAAGC TGCAGAGCCG GCCGGCAGCG CCCCCGGCCC CTGGGCCCGG CCAGCTGACG 
CTGCGTCTGC GGGCCTGCGG GCTCAACTTC GCAGACCTCA TGGCTAGGCA GGGGCTGTAC 
GACCGTCTCC CGCCTCTGCC TGTCACTCCG GGCATGGAGG GCGCGGGTGT TGTGATCGCA 
GTGGGCGAGG GAGTCAGCGA CCGCAAGGCA GGAGACCGGG TGATGGTGTT GAACCGGTCA 
GGGATGTGGC AGGAAGAGGT GACTGTGCCC TCGGTCCAGA CCTTCCTGAT TCCTGAGGCC 
ATGACCTTTG AGGAAGCTGC TGCCTTGCTC GTCAATTACA TTACAGCCTA CATGGTCCTC 
TTTGACTTCG GCAACCTACA GCCTGGCCAC AGCGTCTTGG TACACATGGC TGCAGGGGGT 
GTGGGTATGG CTGCCGTGCA GCTGTGCCGT ACAGTGGAGA ATGTGACAGT GTTCGGAACG 
GCCTCGGCCA GCAAGCACGA GGCACTGAAG GAGAATGGGG TCACACATCC CATCGACTAT 
CACACGACTG ACTACGTGGA TGAGATCAAG AAGATTTCCC CTAAAGGAGT GGACATTGTC 
ATGGACCCTC TGGGTGGGTC AGATACTGCC AAGGGCTACA ACCTCCTGAA ACCCATGGGC 
AAAGTCGTCA CCTATGGAAT GGCCAACCTG CTGACGGGCC CCAAACGGAA CCTGATGGCC 
CTGGCCCGGA CATGGTGGAA TCAGTTCAGC GTGACAGCTC TGCAGCTGCT GCAGGCCAAC 
CGGGCTGTGT GTGGCTTCCA CCTGGGCTAC CTGGATGGTG AGGTGGAGCT GGTCAGTGGT 
GTGGTGGCCC GCCTCCTGGC TCTGTACAAC CAGGGCCACA TCAAGCCCCA CATTGACTCA 
GTCTGGCCCT TCGAGAAGGT GGCTGATGCC ATGAAACAGA TGCAGGAGAA GAAGAATGTG 
GGCAAGGTCC TCCTGGTTCC AGGGCCAGAG AAGGAGAACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

VAT-1 is a novel pathogenic factor of progressive benign prostatic hyperplasia.
The Prostate71(14)1579-86(2011 Oct)
Mori F,Tanigawa K,Endo K,Minamiguchi K,Abe M,Yamada S,Miyoshi K

An essential role for phospholipase D in the recruitment of vesicle amine transport protein-1 to membranes in human neutrophils.
Biochemical pharmacology81(1)144-56(2011 Jan)
Faugaret D,Chouinard FC,Harbour D,El azreq MA,Bourgoin SG

Vesicle amine transport protein-1 (VAT-1) is upregulated in glioblastomas and promotes migration.
Neuropathology and applied neurobiology35(4)342-52(2009 Aug)
Mertsch S,Becker M,Lichota A,Paulus W,Senner V

Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
Journal of proteome research8(3)1304-14(2009 Mar)
Gonzalez-Begne M,Lu B,Han X,Hagen FK,Hand AR,Melvin JE,Yates JR

Large-scale proteomics and phosphoproteomics of urinary exosomes.
Journal of the American Society of Nephrology : JASN20(2)363-79(2009 Feb)
Gonzales PA,Pisitkun T,Hoffert JD,Tchapyjnikov D,Star RA,Kleta R,Wang NS,Knepper MA

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VAT1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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