NXF1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	NXF1
Entrez Gene ID	10482
Full Name	nuclear RNA export factor 1
Synonyms	MEX67,TAP
General protein information	Preferred Names nuclear RNA export factor 1 Names nuclear RNA export factor 1 mRNA export factor TAP tip associating protein tip-associated protein
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 11 Map Location: 11q12.3
Summary	This gene is one member of a family of nuclear RNA export factor genes. Common domain features of this family are a noncanonical RNP-type RNA-binding domain (RBD), 4 leucine-rich repeats (LRRs), a nuclear transport factor 2 (NTF2)-like domain that allows heterodimerization with NTF2-related export protein-1 (NXT1), and a ubiquitin-associated domain that mediates interactions with nucleoporins. The LRRs and NTF2-like domains are required for export activity. Alternative splicing seems to be a common mechanism in this gene family. The encoded protein of this gene shuttles between the nucleus and the cytoplasm and binds in vivo to poly(A)+ RNA. It is the vertebrate homologue of the yeast protein Mex67p. The encoded protein overcomes the mRNA export block caused by the presence of saturating amounts of CTE (constitutive transport element) RNA of type D retroviruses. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2008].
Disorder MIM:	602647

mRNA and Protein(s)

mRNA	Protein	Name
NM_006362.4	NP_006353.2	nuclear RNA export factor 1 isoform 1
NM_001081491.1	NP_001074960.1	nuclear RNA export factor 1 isoform 2
NM_006362.5	NP_006353.2	nuclear RNA export factor 1 isoform 1
NM_001081491.2	NP_001074960.1	nuclear RNA export factor 1 isoform 2

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Nxf1	NP_058093.2
Drosophila melanogaster (fruit fly)	sbr	NP_524660.1
Saccharomyces cerevisiae (baker's yeast)	MEX67	NP_015156.1
Rattus norvegicus (Norway rat)	Nxf1	NP_067590.1
Danio rerio (zebrafish)	nxf1	XP_001923961.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002193	XP_307993.5
Eremothecium gossypii	AGOS_AAL104C	NP_982438.1
Pan troglodytes (chimpanzee)	NXF1	XP_001157986.1
	NXF1	XP_001115982.1
Bos taurus (cattle)	NXF1	NP_001069023.1
Caenorhabditis elegans (roundworm)	nxf-1	NP_506567.1
Schizosaccharomyces pombe (fission yeast)	mex67	NP_595996.1
Xenopus tropicalis (tropical clawed frog)	nxf1	NP_001039200.1
Homo sapiens (human)	NXF1	NP_006353.2
Canis lupus familiaris (dog)	NXF1	XP_540901.4
Caenorhabditis elegans (roundworm)	nxf-2	NP_506568.2
Kluyveromyces lactis	KLLA0C09801g	XP_452635.1

Gene Ontology
Bibliography

Related articles in PubMed

YTHDC1 mediates nuclear export of N
Roundtree IA, Luo GZ, Zhang Z, Wang X, Zhou T, Cui Y, Sha J, Huang X, Guerrero L, Xie P, He E, Shen B, He C
eLife6(2017 Oct)

SRSF1-dependent nuclear export inhibition of C9ORF72 repeat transcripts prevents neurodegeneration and associated motor deficits.
Hautbergue GM, Castelli LM, Ferraiuolo L, Sanchez-Martinez A, Cooper-Knock J, Higginbottom A, Lin YH, Bauer CS, Dodd JE, Myszczynska MA, Alam SM, Garneret P, Chandran JS, Karyka E, Stopford MJ, Smith EF, Kirby J, Meyer K, Kaspar BK, Isaacs AM, El-Khamisy SF, De Vos KJ, Ning K, Azzouz M, Whitworth AJ, Shaw PJ
Nature communications816063(2017 Jul)

RNAi Screen and Proteomics Reveal NXF1 as a Novel Regulator of IRF5 Signaling.
Fu B, Zhao M, Wang L, Patil G, Smith JA, Juncadella IJ, Zuvela-Jelaska L, Dorf ME, Li S
Scientific reports7(1)2683(2017 Jun)

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

small hairpin RNA-mediated down-regulation of TAP or Aly reduced nuclear export of HDAg-L and assembly of HDV virions. Furthermore, a peptide, TAT-HDAg-L(198-210), containing the 10-amino acid TAT peptide and HDAg-L(198-210), inhibited the interaction between HDAg-L and TAP and blocked HDV virion assembly and secretion.
Title: Cellular Nuclear Export Factors TAP and Aly Are Required for HDAg-L-mediated Assembly of Hepatitis Delta Virus.

Complementary structural, biochemical and cellular techniques indicated that the formation of a symmetric RNA binding platform generated by dimerization of NXF1:NXT1 facilitates the recognition of CTE-RNA and promotes its nuclear export
Title: The principal mRNA nuclear export factor NXF1:NXT1 forms a symmetric binding platform that facilitates export of retroviral CTE-RNA.

Gag polyprotein synthesis was decreased by NXF1 knockdown.
Title: Gammaretroviral pol sequences act in cis to direct polysome loading and NXF1/NXT-dependent protein production by gag-encoded RNA.

Hepatitis B virus core protein associates with the cellular NXF1-p15 complex via the nuclear export signal motif of hepatitis B virus core protein.Nuclear export of human hepatitis B virus core protein and pregenomic RNA depends on the NXF1-p15 machinery.
Title: Nuclear export of human hepatitis B virus core protein and pregenomic RNA depends on the cellular NXF1-p15 machinery.

a spatial map is produced in living cells of the sites for the interaction of two TREX subunits, Alyref and Chtop, with Nxf1.
Title: Mapping interactions between mRNA export factors in living cells.

The following NXF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NXF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu05516	NM_006362.5 Latest version!	Homo sapiens nuclear RNA export factor 1 (NXF1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$219.50-$307.30 $439.00
OHu13418	NM_001081491.2 Latest version!	Homo sapiens nuclear RNA export factor 1 (NXF1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$209.30 $299.00
View Old Accession Versions >>
OHu05516	NM_006362.4	Homo sapiens nuclear RNA export factor 1 (NXF1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$219.50-$307.30 $439.00
OHu13418	NM_001081491.1	Homo sapiens nuclear RNA export factor 1 (NXF1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$209.30 $299.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NXF1 ( NM_006362.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_006353.2 Homo sapiens nuclear RNA export factor 1 (NXF1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu05516
Clone ID Related Accession (Same CDS sequence)	NM_006362.4 , NM_006362.5
Accession Version	NM_006362.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1860bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-26
Organism	Homo sapiens(human)
Product	nuclear RNA export factor 1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AP001160.4, BC004904.2, BC028041.1 and AW005685.1. On Feb 9, 2007 this sequence version replaced NM_006362.3. Transcript Variant: This variant (1) encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.183837.1, SRR1660803.259807.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGACG AGGGGAAGTC GTACAGCGAA CACGATGATG AACGCGTTAA TTTCCCTCAA 
AGAAAGAAGA AAGGCCGGGG TCCCTTCCGG TGGAAATATG GTGAAGGAAA CCGTAGGTCT 
GGAAGAGGCG GTTCTGGTAT TCGGTCTTCC CGCCTTGAGG AAGATGATGG AGATGTGGCA 
ATGAGTGATG CCCAGGATGG TCCCCGAGTA CGATACAACC CCTATACCAC CCGACCTAAC 
CGTCGGGGTG ATACTTGGCA TGATCGAGAT CGCATTCATG TTACTGTGCG GAGAGACAGA 
GCTCCTCCAG AGAGAGGAGG GGCTGGCACC AGCCAGGATG GGACCTCAAA GAACTGGTTC 
AAGATTACAA TTCCTTATGG CAGAAAGTAT GACAAGGCAT GGCTCCTGAG CATGATTCAG 
AGCAAGTGCA GTGTGCCCTT CACCCCTATT GAGTTTCACT ATGAGAATAC ACGGGCCCAG 
TTCTTCGTTG AAGACGCCAG TACTGCCTCT GCATTGAAGG CTGTCAACTA TAAGATTTTG 
GATCGGGAGA ACCGAAGGAT ATCTATCATC ATCAACTCTT CTGCTCCACC CCACACTATA 
CTGAATGAAC TGAAGCCAGA ACAAGTAGAA CAGCTAAAGC TGATCATGAG CAAACGATAC 
GATGGCTCCC AACAAGCCCT TGACCTCAAA GGCCTCCGTT CAGACCCAGA TTTGGTGGCC 
CAGAACATTG ACGTTGTCCT GAATCGCAGA AGCTGTATGG CAGCTACCCT GAGGATCATT 
GAAGAGAACA TCCCTGAGCT ATTGTCCTTG AACTTGAGCA ACAACAGGCT GTACAGGCTG 
GATGACATGT CTAGCATTGT TCAGAAGGCA CCCAACCTGA AGATCCTAAA CCTTTCTGGA 
AATGAATTGA AGTCTGAGCG GGAATTGGAC AAGATAAAGG GGCTGAAGCT AGAAGAGCTC 
TGGCTCGATG GAAACTCCCT GTGTGACACC TTCCGAGACC AGTCCACCTA CATCAGCGCC 
ATTCGCGAAC GATTTCCCAA GTTACTACGC CTGGATGGCC ATGAGCTACC CCCACCAATT 
GCCTTTGATG TTGAAGCCCC CACGACGTTA CCGCCCTGCA AGGGAAGCTA TTTTGGAACA 
GAAAACTTGA AGAGTCTGGT CTTGCACTTC CTGCAACAGT ACTATGCAAT TTACGACTCT 
GGAGACCGAC AAGGGCTCCT GGATGCCTAC CATGATGGGG CCTGCTGTTC CCTGAGCATT 
CCTTTCATTC CTCAGAACCC TGCCCGAAGC AGCTTAGCCG AGTATTTCAA GGATAGCAGA 
AATGTGAAGA AGCTTAAAGA CCCTACCTTG CGGTTCCGGC TGCTGAAGCA CACGCGTCTC 
AACGTTGTTG CCTTCCTCAA TGAGTTGCCC AAAACCCAGC ACGACGTCAA TTCCTTCGTG 
GTAGACATAA GCGCCCAGAC AAGCACATTG CTGTGTTTTT CTGTCAATGG AGTCTTCAAG 
GAAGTGGACG GAAAGTCCCG GGATTCTTTG CGAGCCTTCA CCCGGACATT CATTGCTGTT 
CCTGCTAGCA ATTCAGGGCT ATGTATTGTA AATGATGAGC TATTTGTGCG GAATGCCAGT 
TCTGAAGAGA TCCAAAGAGC CTTCGCTATG CCTGCACCCA CGCCTTCCTC CAGCCCGGTG 
CCCACCCTCT CTCCAGAGCA GCAGGAAATG TTGCAAGCAT TCTCTACCCA GTCTGGCATG 
AACCTCGAGT GGTCCCAGAA GTGCCTTCAG GACAACAACT GGGACTACAC CAGATCTGCC 
CAGGCCTTCA CTCATCTCAA GGCCAAGGGC GAGATCCCAG AAGTGGCATT CATGAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_006353.2
CDS	137..1996
Translation	MADEGKSYSEHDDERVNFPQRKKKGRGPFRWKYGEGNRRSGRGGSGIRSSRLEEDDGDVAMSDAQDGPRVRYNPYTTRPNRRGDTWHDRDRIHVTVRRDRAPPERGGAGTSQDGTSKNWFKITIPYGRKYDKAWLLSMIQSKCSVPFTPIEFHYENTRAQFFVEDASTASALKAVNYKILDRENRRISIIINSSAPPHTILNELKPEQVEQLKLIMSKRYDGSQQALDLKGLRSDPDLVAQNIDVVLNRRSCMAATLRIIEENIPELLSLNLSNNRLYRLDDMSSIVQKAPNLKILNLSGNELKSERELDKIKGLKLEELWLDGNSLCDTFRDQSTYISAIRERFPKLLRLDGHELPPPIAFDVEAPTTLPPCKGSYFGTENLKSLVLHFLQQYYAIYDSGDRQGLLDAYHDGACCSLSIPFIPQNPARSSLAEYFKDSRNVKKLKDPTLRFRLLKHTRLNVVAFLNELPKTQHDVNSFVVDISAQTSTLLCFSVNGVFKEVDGKSRDSLRAFTRTFIAVPASNSGLCIVNDELFVRNASSEEIQRAFAMPAPTPSSSPVPTLSPEQQEMLQAFSTQSGMNLEWSQKCLQDNNWDYTRSAQAFTHLKAKGEIPEVAFMK

Target ORF information:

RefSeq Version	NM_006362.4
Organism	Homo sapiens(human)
Definition	Homo sapiens nuclear RNA export factor 1 (NXF1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006362.4

ORF Insert Sequence:

ATGGCGGACG AGGGGAAGTC GTACAGCGAA CACGATGATG AACGCGTTAA TTTCCCTCAA 
AGAAAGAAGA AAGGCCGGGG TCCCTTCCGG TGGAAATATG GTGAAGGAAA CCGTAGGTCT 
GGAAGAGGCG GTTCTGGTAT TCGGTCTTCC CGCCTTGAGG AAGATGATGG AGATGTGGCA 
ATGAGTGATG CCCAGGATGG TCCCCGAGTA CGATACAACC CCTATACCAC CCGACCTAAC 
CGTCGGGGTG ATACTTGGCA TGATCGAGAT CGCATTCATG TTACTGTGCG GAGAGACAGA 
GCTCCTCCAG AGAGAGGAGG GGCTGGCACC AGCCAGGATG GGACCTCAAA GAACTGGTTC 
AAGATTACAA TTCCTTATGG CAGAAAGTAT GACAAGGCAT GGCTCCTGAG CATGATTCAG 
AGCAAGTGCA GTGTGCCCTT CACCCCTATT GAGTTTCACT ATGAGAATAC ACGGGCCCAG 
TTCTTCGTTG AAGACGCCAG TACTGCCTCT GCATTGAAGG CTGTCAACTA TAAGATTTTG 
GATCGGGAGA ACCGAAGGAT ATCTATCATC ATCAACTCTT CTGCTCCACC CCACACTATA 
CTGAATGAAC TGAAGCCAGA ACAAGTAGAA CAGCTAAAGC TGATCATGAG CAAACGATAC 
GATGGCTCCC AACAAGCCCT TGACCTCAAA GGCCTCCGTT CAGACCCAGA TTTGGTGGCC 
CAGAACATTG ACGTTGTCCT GAATCGCAGA AGCTGTATGG CAGCTACCCT GAGGATCATT 
GAAGAGAACA TCCCTGAGCT ATTGTCCTTG AACTTGAGCA ACAACAGGCT GTACAGGCTG 
GATGACATGT CTAGCATTGT TCAGAAGGCA CCCAACCTGA AGATCCTAAA CCTTTCTGGA 
AATGAATTGA AGTCTGAGCG GGAATTGGAC AAGATAAAGG GGCTGAAGCT AGAAGAGCTC 
TGGCTCGATG GAAACTCCCT GTGTGACACC TTCCGAGACC AGTCCACCTA CATCAGCGCC 
ATTCGCGAAC GATTTCCCAA GTTACTACGC CTGGATGGCC ATGAGCTACC CCCACCAATT 
GCCTTTGATG TTGAAGCCCC CACGACGTTA CCGCCCTGCA AGGGAAGCTA TTTTGGAACA 
GAAAACTTGA AGAGTCTGGT CTTGCACTTC CTGCAACAGT ACTATGCAAT TTACGACTCT 
GGAGACCGAC AAGGGCTCCT GGATGCCTAC CATGATGGGG CCTGCTGTTC CCTGAGCATT 
CCTTTCATTC CTCAGAACCC TGCCCGAAGC AGCTTAGCCG AGTATTTCAA GGATAGCAGA 
AATGTGAAGA AGCTTAAAGA CCCTACCTTG CGGTTCCGGC TGCTGAAGCA CACGCGTCTC 
AACGTTGTTG CCTTCCTCAA TGAGTTGCCC AAAACCCAGC ACGACGTCAA TTCCTTCGTG 
GTAGACATAA GCGCCCAGAC AAGCACATTG CTGTGTTTTT CTGTCAATGG AGTCTTCAAG 
GAAGTGGACG GAAAGTCCCG GGATTCTTTG CGAGCCTTCA CCCGGACATT CATTGCTGTT 
CCTGCTAGCA ATTCAGGGCT ATGTATTGTA AATGATGAGC TATTTGTGCG GAATGCCAGT 
TCTGAAGAGA TCCAAAGAGC CTTCGCTATG CCTGCACCCA CGCCTTCCTC CAGCCCGGTG 
CCCACCCTCT CTCCAGAGCA GCAGGAAATG TTGCAAGCAT TCTCTACCCA GTCTGGCATG 
AACCTCGAGT GGTCCCAGAA GTGCCTTCAG GACAACAACT GGGACTACAC CAGATCTGCC 
CAGGCCTTCA CTCATCTCAA GGCCAAGGGC GAGATCCCAG AAGTGGCATT CATGAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NXF1 ( NM_006362.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_006353.2 Homo sapiens nuclear RNA export factor 1 (NXF1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu05516
Clone ID Related Accession (Same CDS sequence)	NM_006362.4 , NM_006362.5
Accession Version	NM_006362.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1860bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	nuclear RNA export factor 1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AP001160.4, BC004904.2 and BC028041.1. On Nov 22, 2018 this sequence version replaced NM_006362.4. Transcript Variant: This variant (1) encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.586984.1, SRR3476690.243406.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000294172.7/ ENSP00000294172.2 RefSeq Select criteria :: based on conservation, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCGGACG AGGGGAAGTC GTACAGCGAA CACGATGATG AACGCGTTAA TTTCCCTCAA 
AGAAAGAAGA AAGGCCGGGG TCCCTTCCGG TGGAAATATG GTGAAGGAAA CCGTAGGTCT 
GGAAGAGGCG GTTCTGGTAT TCGGTCTTCC CGCCTTGAGG AAGATGATGG AGATGTGGCA 
ATGAGTGATG CCCAGGATGG TCCCCGAGTA CGATACAACC CCTATACCAC CCGACCTAAC 
CGTCGGGGTG ATACTTGGCA TGATCGAGAT CGCATTCATG TTACTGTGCG GAGAGACAGA 
GCTCCTCCAG AGAGAGGAGG GGCTGGCACC AGCCAGGATG GGACCTCAAA GAACTGGTTC 
AAGATTACAA TTCCTTATGG CAGAAAGTAT GACAAGGCAT GGCTCCTGAG CATGATTCAG 
AGCAAGTGCA GTGTGCCCTT CACCCCTATT GAGTTTCACT ATGAGAATAC ACGGGCCCAG 
TTCTTCGTTG AAGACGCCAG TACTGCCTCT GCATTGAAGG CTGTCAACTA TAAGATTTTG 
GATCGGGAGA ACCGAAGGAT ATCTATCATC ATCAACTCTT CTGCTCCACC CCACACTATA 
CTGAATGAAC TGAAGCCAGA ACAAGTAGAA CAGCTAAAGC TGATCATGAG CAAACGATAC 
GATGGCTCCC AACAAGCCCT TGACCTCAAA GGCCTCCGTT CAGACCCAGA TTTGGTGGCC 
CAGAACATTG ACGTTGTCCT GAATCGCAGA AGCTGTATGG CAGCTACCCT GAGGATCATT 
GAAGAGAACA TCCCTGAGCT ATTGTCCTTG AACTTGAGCA ACAACAGGCT GTACAGGCTG 
GATGACATGT CTAGCATTGT TCAGAAGGCA CCCAACCTGA AGATCCTAAA CCTTTCTGGA 
AATGAATTGA AGTCTGAGCG GGAATTGGAC AAGATAAAGG GGCTGAAGCT AGAAGAGCTC 
TGGCTCGATG GAAACTCCCT GTGTGACACC TTCCGAGACC AGTCCACCTA CATCAGCGCC 
ATTCGCGAAC GATTTCCCAA GTTACTACGC CTGGATGGCC ATGAGCTACC CCCACCAATT 
GCCTTTGATG TTGAAGCCCC CACGACGTTA CCGCCCTGCA AGGGAAGCTA TTTTGGAACA 
GAAAACTTGA AGAGTCTGGT CTTGCACTTC CTGCAACAGT ACTATGCAAT TTACGACTCT 
GGAGACCGAC AAGGGCTCCT GGATGCCTAC CATGATGGGG CCTGCTGTTC CCTGAGCATT 
CCTTTCATTC CTCAGAACCC TGCCCGAAGC AGCTTAGCCG AGTATTTCAA GGATAGCAGA 
AATGTGAAGA AGCTTAAAGA CCCTACCTTG CGGTTCCGGC TGCTGAAGCA CACGCGTCTC 
AACGTTGTTG CCTTCCTCAA TGAGTTGCCC AAAACCCAGC ACGACGTCAA TTCCTTCGTG 
GTAGACATAA GCGCCCAGAC AAGCACATTG CTGTGTTTTT CTGTCAATGG AGTCTTCAAG 
GAAGTGGACG GAAAGTCCCG GGATTCTTTG CGAGCCTTCA CCCGGACATT CATTGCTGTT 
CCTGCTAGCA ATTCAGGGCT ATGTATTGTA AATGATGAGC TATTTGTGCG GAATGCCAGT 
TCTGAAGAGA TCCAAAGAGC CTTCGCTATG CCTGCACCCA CGCCTTCCTC CAGCCCGGTG 
CCCACCCTCT CTCCAGAGCA GCAGGAAATG TTGCAAGCAT TCTCTACCCA GTCTGGCATG 
AACCTCGAGT GGTCCCAGAA GTGCCTTCAG GACAACAACT GGGACTACAC CAGATCTGCC 
CAGGCCTTCA CTCATCTCAA GGCCAAGGGC GAGATCCCAG AAGTGGCATT CATGAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_006353.2
CDS	85..1944
Translation	MADEGKSYSEHDDERVNFPQRKKKGRGPFRWKYGEGNRRSGRGGSGIRSSRLEEDDGDVAMSDAQDGPRVRYNPYTTRPNRRGDTWHDRDRIHVTVRRDRAPPERGGAGTSQDGTSKNWFKITIPYGRKYDKAWLLSMIQSKCSVPFTPIEFHYENTRAQFFVEDASTASALKAVNYKILDRENRRISIIINSSAPPHTILNELKPEQVEQLKLIMSKRYDGSQQALDLKGLRSDPDLVAQNIDVVLNRRSCMAATLRIIEENIPELLSLNLSNNRLYRLDDMSSIVQKAPNLKILNLSGNELKSERELDKIKGLKLEELWLDGNSLCDTFRDQSTYISAIRERFPKLLRLDGHELPPPIAFDVEAPTTLPPCKGSYFGTENLKSLVLHFLQQYYAIYDSGDRQGLLDAYHDGACCSLSIPFIPQNPARSSLAEYFKDSRNVKKLKDPTLRFRLLKHTRLNVVAFLNELPKTQHDVNSFVVDISAQTSTLLCFSVNGVFKEVDGKSRDSLRAFTRTFIAVPASNSGLCIVNDELFVRNASSEEIQRAFAMPAPTPSSSPVPTLSPEQQEMLQAFSTQSGMNLEWSQKCLQDNNWDYTRSAQAFTHLKAKGEIPEVAFMK

Target ORF information:

RefSeq Version	NM_006362.5
Organism	Homo sapiens(human)
Definition	Homo sapiens nuclear RNA export factor 1 (NXF1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006362.5

ORF Insert Sequence:

ATGGCGGACG AGGGGAAGTC GTACAGCGAA CACGATGATG AACGCGTTAA TTTCCCTCAA 
AGAAAGAAGA AAGGCCGGGG TCCCTTCCGG TGGAAATATG GTGAAGGAAA CCGTAGGTCT 
GGAAGAGGCG GTTCTGGTAT TCGGTCTTCC CGCCTTGAGG AAGATGATGG AGATGTGGCA 
ATGAGTGATG CCCAGGATGG TCCCCGAGTA CGATACAACC CCTATACCAC CCGACCTAAC 
CGTCGGGGTG ATACTTGGCA TGATCGAGAT CGCATTCATG TTACTGTGCG GAGAGACAGA 
GCTCCTCCAG AGAGAGGAGG GGCTGGCACC AGCCAGGATG GGACCTCAAA GAACTGGTTC 
AAGATTACAA TTCCTTATGG CAGAAAGTAT GACAAGGCAT GGCTCCTGAG CATGATTCAG 
AGCAAGTGCA GTGTGCCCTT CACCCCTATT GAGTTTCACT ATGAGAATAC ACGGGCCCAG 
TTCTTCGTTG AAGACGCCAG TACTGCCTCT GCATTGAAGG CTGTCAACTA TAAGATTTTG 
GATCGGGAGA ACCGAAGGAT ATCTATCATC ATCAACTCTT CTGCTCCACC CCACACTATA 
CTGAATGAAC TGAAGCCAGA ACAAGTAGAA CAGCTAAAGC TGATCATGAG CAAACGATAC 
GATGGCTCCC AACAAGCCCT TGACCTCAAA GGCCTCCGTT CAGACCCAGA TTTGGTGGCC 
CAGAACATTG ACGTTGTCCT GAATCGCAGA AGCTGTATGG CAGCTACCCT GAGGATCATT 
GAAGAGAACA TCCCTGAGCT ATTGTCCTTG AACTTGAGCA ACAACAGGCT GTACAGGCTG 
GATGACATGT CTAGCATTGT TCAGAAGGCA CCCAACCTGA AGATCCTAAA CCTTTCTGGA 
AATGAATTGA AGTCTGAGCG GGAATTGGAC AAGATAAAGG GGCTGAAGCT AGAAGAGCTC 
TGGCTCGATG GAAACTCCCT GTGTGACACC TTCCGAGACC AGTCCACCTA CATCAGCGCC 
ATTCGCGAAC GATTTCCCAA GTTACTACGC CTGGATGGCC ATGAGCTACC CCCACCAATT 
GCCTTTGATG TTGAAGCCCC CACGACGTTA CCGCCCTGCA AGGGAAGCTA TTTTGGAACA 
GAAAACTTGA AGAGTCTGGT CTTGCACTTC CTGCAACAGT ACTATGCAAT TTACGACTCT 
GGAGACCGAC AAGGGCTCCT GGATGCCTAC CATGATGGGG CCTGCTGTTC CCTGAGCATT 
CCTTTCATTC CTCAGAACCC TGCCCGAAGC AGCTTAGCCG AGTATTTCAA GGATAGCAGA 
AATGTGAAGA AGCTTAAAGA CCCTACCTTG CGGTTCCGGC TGCTGAAGCA CACGCGTCTC 
AACGTTGTTG CCTTCCTCAA TGAGTTGCCC AAAACCCAGC ACGACGTCAA TTCCTTCGTG 
GTAGACATAA GCGCCCAGAC AAGCACATTG CTGTGTTTTT CTGTCAATGG AGTCTTCAAG 
GAAGTGGACG GAAAGTCCCG GGATTCTTTG CGAGCCTTCA CCCGGACATT CATTGCTGTT 
CCTGCTAGCA ATTCAGGGCT ATGTATTGTA AATGATGAGC TATTTGTGCG GAATGCCAGT 
TCTGAAGAGA TCCAAAGAGC CTTCGCTATG CCTGCACCCA CGCCTTCCTC CAGCCCGGTG 
CCCACCCTCT CTCCAGAGCA GCAGGAAATG TTGCAAGCAT TCTCTACCCA GTCTGGCATG 
AACCTCGAGT GGTCCCAGAA GTGCCTTCAG GACAACAACT GGGACTACAC CAGATCTGCC 
CAGGCCTTCA CTCATCTCAA GGCCAAGGGC GAGATCCCAG AAGTGGCATT CATGAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NXF1 ( NM_001081491.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001074960.1 Homo sapiens nuclear RNA export factor 1 (NXF1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu13418
Clone ID Related Accession (Same CDS sequence)	NM_001081491.1 , NM_001081491.2
Accession Version	NM_001081491.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1071bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-16
Organism	Homo sapiens(human)
Product	nuclear RNA export factor 1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB141140.1, AB209915.1 and AV750251.1. Transcript Variant: This variant (2) includes an alternate segment, compared to variant 1, resulting in a shorter protein (isoform 2) that has a distinct C-terminus, compared to isoform 1. CCDS Note: This CCDS ID represents the protein described in PMID: 16971948. This transcript is supported by AB209915.1. It should be noted this transcript is predicted to undergo nonsense-mediated mRNA decay (NMD). However, the protein is represented because it was detected endogenously in PMID: 16971948. It is likely that the majority of transcripts representing this variant will undergo NMD, while some low level of NMD escape may allow for the expression of this protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.244731.1, SRR1803616.2324.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## NMD candidate :: PMID: 16971948 ##RefSeq-Attributes-END##

ATGGCGGACG AGGGGAAGTC GTACAGCGAA CACGATGATG AACGCGTTAA TTTCCCTCAA 
AGAAAGAAGA AAGGCCGGGG TCCCTTCCGG TGGAAATATG GTGAAGGAAA CCGTAGGTCT 
GGAAGAGGCG GTTCTGGTAT TCGGTCTTCC CGCCTTGAGG AAGATGATGG AGATGTGGCA 
ATGAGTGATG CCCAGGATGG TCCCCGAGTA CGATACAACC CCTATACCAC CCGACCTAAC 
CGTCGGGGTG ATACTTGGCA TGATCGAGAT CGCATTCATG TTACTGTGCG GAGAGACAGA 
GCTCCTCCAG AGAGAGGAGG GGCTGGCACC AGCCAGGATG GGACCTCAAA GAACTGGTTC 
AAGATTACAA TTCCTTATGG CAGAAAGTAT GACAAGGCAT GGCTCCTGAG CATGATTCAG 
AGCAAGTGCA GTGTGCCCTT CACCCCTATT GAGTTTCACT ATGAGAATAC ACGGGCCCAG 
TTCTTCGTTG AAGACGCCAG TACTGCCTCT GCATTGAAGG CTGTCAACTA TAAGATTTTG 
GATCGGGAGA ACCGAAGGAT ATCTATCATC ATCAACTCTT CTGCTCCACC CCACACTATA 
CTGAATGAAC TGAAGCCAGA ACAAGTAGAA CAGCTAAAGC TGATCATGAG CAAACGATAC 
GATGGCTCCC AACAAGCCCT TGACCTCAAA GGCCTCCGTT CAGACCCAGA TTTGGTGGCC 
CAGAACATTG ACGTTGTCCT GAATCGCAGA AGCTGTATGG CAGCTACCCT GAGGATCATT 
GAAGAGAACA TCCCTGAGCT ATTGTCCTTG AACTTGAGCA ACAACAGGCT GTACAGGCTG 
GATGACATGT CTAGCATTGT TCAGAAGGCA CCCAACCTGA AGATCCTAAA CCTTTCTGGA 
AATGAATTGA AGTCTGAGCG GGAATTGGAC AAGATAAAGG GGCTGAAGCT AGAAGAGCTC 
TGGCTCGATG GAAACTCCCT GTGTGACACC TTCCGAGACC AGTCCACCTA CATCAGGTCA 
GTTGTAGCCT GTGTCTCCCC TCCTGGGGAC CTTCACCCCC TGGGAGGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001074960.1
CDS	137..1207
Translation	MADEGKSYSEHDDERVNFPQRKKKGRGPFRWKYGEGNRRSGRGGSGIRSSRLEEDDGDVAMSDAQDGPRVRYNPYTTRPNRRGDTWHDRDRIHVTVRRDRAPPERGGAGTSQDGTSKNWFKITIPYGRKYDKAWLLSMIQSKCSVPFTPIEFHYENTRAQFFVEDASTASALKAVNYKILDRENRRISIIINSSAPPHTILNELKPEQVEQLKLIMSKRYDGSQQALDLKGLRSDPDLVAQNIDVVLNRRSCMAATLRIIEENIPELLSLNLSNNRLYRLDDMSSIVQKAPNLKILNLSGNELKSERELDKIKGLKLEELWLDGNSLCDTFRDQSTYIRSVVACVSPPGDLHPLGG

Target ORF information:

RefSeq Version	NM_001081491.1
Organism	Homo sapiens(human)
Definition	Homo sapiens nuclear RNA export factor 1 (NXF1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001081491.1

ORF Insert Sequence:

ATGGCGGACG AGGGGAAGTC GTACAGCGAA CACGATGATG AACGCGTTAA TTTCCCTCAA 
AGAAAGAAGA AAGGCCGGGG TCCCTTCCGG TGGAAATATG GTGAAGGAAA CCGTAGGTCT 
GGAAGAGGCG GTTCTGGTAT TCGGTCTTCC CGCCTTGAGG AAGATGATGG AGATGTGGCA 
ATGAGTGATG CCCAGGATGG TCCCCGAGTA CGATACAACC CCTATACCAC CCGACCTAAC 
CGTCGGGGTG ATACTTGGCA TGATCGAGAT CGCATTCATG TTACTGTGCG GAGAGACAGA 
GCTCCTCCAG AGAGAGGAGG GGCTGGCACC AGCCAGGATG GGACCTCAAA GAACTGGTTC 
AAGATTACAA TTCCTTATGG CAGAAAGTAT GACAAGGCAT GGCTCCTGAG CATGATTCAG 
AGCAAGTGCA GTGTGCCCTT CACCCCTATT GAGTTTCACT ATGAGAATAC ACGGGCCCAG 
TTCTTCGTTG AAGACGCCAG TACTGCCTCT GCATTGAAGG CTGTCAACTA TAAGATTTTG 
GATCGGGAGA ACCGAAGGAT ATCTATCATC ATCAACTCTT CTGCTCCACC CCACACTATA 
CTGAATGAAC TGAAGCCAGA ACAAGTAGAA CAGCTAAAGC TGATCATGAG CAAACGATAC 
GATGGCTCCC AACAAGCCCT TGACCTCAAA GGCCTCCGTT CAGACCCAGA TTTGGTGGCC 
CAGAACATTG ACGTTGTCCT GAATCGCAGA AGCTGTATGG CAGCTACCCT GAGGATCATT 
GAAGAGAACA TCCCTGAGCT ATTGTCCTTG AACTTGAGCA ACAACAGGCT GTACAGGCTG 
GATGACATGT CTAGCATTGT TCAGAAGGCA CCCAACCTGA AGATCCTAAA CCTTTCTGGA 
AATGAATTGA AGTCTGAGCG GGAATTGGAC AAGATAAAGG GGCTGAAGCT AGAAGAGCTC 
TGGCTCGATG GAAACTCCCT GTGTGACACC TTCCGAGACC AGTCCACCTA CATCAGGTCA 
GTTGTAGCCT GTGTCTCCCC TCCTGGGGAC CTTCACCCCC TGGGAGGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NXF1 ( NM_001081491.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001074960.1 Homo sapiens nuclear RNA export factor 1 (NXF1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu13418
Clone ID Related Accession (Same CDS sequence)	NM_001081491.1 , NM_001081491.2
Accession Version	NM_001081491.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1071bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-10
Organism	Homo sapiens(human)
Product	nuclear RNA export factor 1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB141140.1, AB209915.1 and AV750251.1. On May 31, 2019 this sequence version replaced NM_001081491.1. Transcript Variant: This variant (2) includes an alternate segment, compared to variant 1, resulting in a shorter protein (isoform 2) that has a distinct C-terminus, compared to isoform 1. CCDS Note: This CCDS ID represents the protein described in PMID: 16971948. This transcript is supported by AB209915.1. It should be noted this transcript is predicted to undergo nonsense-mediated mRNA decay (NMD). However, the protein is represented because it was detected endogenously in PMID: 16971948. It is likely that the majority of transcripts representing this variant will undergo NMD, while some low level of NMD escape may allow for the expression of this protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.228535.1, SRR1660805.188270.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## NMD candidate :: PMID: 16971948 ##RefSeq-Attributes-END##

ATGGCGGACG AGGGGAAGTC GTACAGCGAA CACGATGATG AACGCGTTAA TTTCCCTCAA 
AGAAAGAAGA AAGGCCGGGG TCCCTTCCGG TGGAAATATG GTGAAGGAAA CCGTAGGTCT 
GGAAGAGGCG GTTCTGGTAT TCGGTCTTCC CGCCTTGAGG AAGATGATGG AGATGTGGCA 
ATGAGTGATG CCCAGGATGG TCCCCGAGTA CGATACAACC CCTATACCAC CCGACCTAAC 
CGTCGGGGTG ATACTTGGCA TGATCGAGAT CGCATTCATG TTACTGTGCG GAGAGACAGA 
GCTCCTCCAG AGAGAGGAGG GGCTGGCACC AGCCAGGATG GGACCTCAAA GAACTGGTTC 
AAGATTACAA TTCCTTATGG CAGAAAGTAT GACAAGGCAT GGCTCCTGAG CATGATTCAG 
AGCAAGTGCA GTGTGCCCTT CACCCCTATT GAGTTTCACT ATGAGAATAC ACGGGCCCAG 
TTCTTCGTTG AAGACGCCAG TACTGCCTCT GCATTGAAGG CTGTCAACTA TAAGATTTTG 
GATCGGGAGA ACCGAAGGAT ATCTATCATC ATCAACTCTT CTGCTCCACC CCACACTATA 
CTGAATGAAC TGAAGCCAGA ACAAGTAGAA CAGCTAAAGC TGATCATGAG CAAACGATAC 
GATGGCTCCC AACAAGCCCT TGACCTCAAA GGCCTCCGTT CAGACCCAGA TTTGGTGGCC 
CAGAACATTG ACGTTGTCCT GAATCGCAGA AGCTGTATGG CAGCTACCCT GAGGATCATT 
GAAGAGAACA TCCCTGAGCT ATTGTCCTTG AACTTGAGCA ACAACAGGCT GTACAGGCTG 
GATGACATGT CTAGCATTGT TCAGAAGGCA CCCAACCTGA AGATCCTAAA CCTTTCTGGA 
AATGAATTGA AGTCTGAGCG GGAATTGGAC AAGATAAAGG GGCTGAAGCT AGAAGAGCTC 
TGGCTCGATG GAAACTCCCT GTGTGACACC TTCCGAGACC AGTCCACCTA CATCAGGTCA 
GTTGTAGCCT GTGTCTCCCC TCCTGGGGAC CTTCACCCCC TGGGAGGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001074960.1
CDS	85..1155
Translation	MADEGKSYSEHDDERVNFPQRKKKGRGPFRWKYGEGNRRSGRGGSGIRSSRLEEDDGDVAMSDAQDGPRVRYNPYTTRPNRRGDTWHDRDRIHVTVRRDRAPPERGGAGTSQDGTSKNWFKITIPYGRKYDKAWLLSMIQSKCSVPFTPIEFHYENTRAQFFVEDASTASALKAVNYKILDRENRRISIIINSSAPPHTILNELKPEQVEQLKLIMSKRYDGSQQALDLKGLRSDPDLVAQNIDVVLNRRSCMAATLRIIEENIPELLSLNLSNNRLYRLDDMSSIVQKAPNLKILNLSGNELKSERELDKIKGLKLEELWLDGNSLCDTFRDQSTYIRSVVACVSPPGDLHPLGG

Target ORF information:

RefSeq Version	NM_001081491.2
Organism	Homo sapiens(human)
Definition	Homo sapiens nuclear RNA export factor 1 (NXF1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001081491.2

ORF Insert Sequence:

ATGGCGGACG AGGGGAAGTC GTACAGCGAA CACGATGATG AACGCGTTAA TTTCCCTCAA 
AGAAAGAAGA AAGGCCGGGG TCCCTTCCGG TGGAAATATG GTGAAGGAAA CCGTAGGTCT 
GGAAGAGGCG GTTCTGGTAT TCGGTCTTCC CGCCTTGAGG AAGATGATGG AGATGTGGCA 
ATGAGTGATG CCCAGGATGG TCCCCGAGTA CGATACAACC CCTATACCAC CCGACCTAAC 
CGTCGGGGTG ATACTTGGCA TGATCGAGAT CGCATTCATG TTACTGTGCG GAGAGACAGA 
GCTCCTCCAG AGAGAGGAGG GGCTGGCACC AGCCAGGATG GGACCTCAAA GAACTGGTTC 
AAGATTACAA TTCCTTATGG CAGAAAGTAT GACAAGGCAT GGCTCCTGAG CATGATTCAG 
AGCAAGTGCA GTGTGCCCTT CACCCCTATT GAGTTTCACT ATGAGAATAC ACGGGCCCAG 
TTCTTCGTTG AAGACGCCAG TACTGCCTCT GCATTGAAGG CTGTCAACTA TAAGATTTTG 
GATCGGGAGA ACCGAAGGAT ATCTATCATC ATCAACTCTT CTGCTCCACC CCACACTATA 
CTGAATGAAC TGAAGCCAGA ACAAGTAGAA CAGCTAAAGC TGATCATGAG CAAACGATAC 
GATGGCTCCC AACAAGCCCT TGACCTCAAA GGCCTCCGTT CAGACCCAGA TTTGGTGGCC 
CAGAACATTG ACGTTGTCCT GAATCGCAGA AGCTGTATGG CAGCTACCCT GAGGATCATT 
GAAGAGAACA TCCCTGAGCT ATTGTCCTTG AACTTGAGCA ACAACAGGCT GTACAGGCTG 
GATGACATGT CTAGCATTGT TCAGAAGGCA CCCAACCTGA AGATCCTAAA CCTTTCTGGA 
AATGAATTGA AGTCTGAGCG GGAATTGGAC AAGATAAAGG GGCTGAAGCT AGAAGAGCTC 
TGGCTCGATG GAAACTCCCT GTGTGACACC TTCCGAGACC AGTCCACCTA CATCAGGTCA 
GTTGTAGCCT GTGTCTCCCC TCCTGGGGAC CTTCACCCCC TGGGAGGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

YTHDC1 mediates nuclear export of N
eLife6(2017 Oct)
Roundtree IA,Luo GZ,Zhang Z,Wang X,Zhou T,Cui Y,Sha J,Huang X,Guerrero L,Xie P,He E,Shen B,He C

SRSF1-dependent nuclear export inhibition of C9ORF72 repeat transcripts prevents neurodegeneration and associated motor deficits.
Nature communications816063(2017 Jul)
Hautbergue GM,Castelli LM,Ferraiuolo L,Sanchez-Martinez A,Cooper-Knock J,Higginbottom A,Lin YH,Bauer CS,Dodd JE,Myszczynska MA,Alam SM,Garneret P,Chandran JS,Karyka E,Stopford MJ,Smith EF,Kirby J,Meyer K,Kaspar BK,Isaacs AM,El-Khamisy SF,De Vos KJ,Ning K,Azzouz M,Whitworth AJ,Shaw PJ

RNAi Screen and Proteomics Reveal NXF1 as a Novel Regulator of IRF5 Signaling.
Scientific reports7(1)2683(2017 Jun)
Fu B,Zhao M,Wang L,Patil G,Smith JA,Juncadella IJ,Zuvela-Jelaska L,Dorf ME,Li S

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

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NXF1 cDNA ORF clone, Homo sapiens(human)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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