PEMT cDNA ORF clone, Homo sapiens(human)

The following PEMT gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PEMT cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu10059 NM_148172.3
Latest version!
Homo sapiens phosphatidylethanolamine N-methyltransferase (PEMT), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$99.50-$139.30
$199.00
OHu13080 NM_007169.3
Latest version!
Homo sapiens phosphatidylethanolamine N-methyltransferase (PEMT), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$49.50-$69.30
$99.00
OHu13080 NM_148173.2
Latest version!
Homo sapiens phosphatidylethanolamine N-methyltransferase (PEMT), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$49.50-$69.30
$99.00
OHu13080 XM_024450532.1
Latest version!
Homo sapiens phosphatidylethanolamine N-methyltransferase (PEMT), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$49.50-$69.30
$99.00
OHu09624 NM_001267552.2
Latest version!
Homo sapiens phosphatidylethanolamine N-methyltransferase (PEMT), transcript variant 5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $139.30
$199.00
OHu09679 NM_001267551.2
Latest version!
Homo sapiens phosphatidylethanolamine N-methyltransferase (PEMT), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $139.30
$199.00
OHu63981 XM_006721418.4
Latest version!
Homo sapiens phosphatidylethanolamine N-methyltransferase (PEMT), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $139.30
$199.00
OHu86479 XM_017024016.1
Latest version!
Homo sapiens phosphatidylethanolamine N-methyltransferase (PEMT), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $69.30
$99.00
View Old Accession Versions >>
OHu13080 NM_148173.1 Homo sapiens phosphatidylethanolamine N-methyltransferase (PEMT), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$49.50-$69.30
$99.00
OHu10059 NM_148172.2 Homo sapiens phosphatidylethanolamine N-methyltransferase (PEMT), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $188.30
$269.00
OHu09624 NM_001267552.1 Homo sapiens phosphatidylethanolamine N-methyltransferase (PEMT), transcript variant 5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $139.30
$199.00
OHu13080 NM_007169.2 Homo sapiens phosphatidylethanolamine N-methyltransferase (PEMT), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$49.50-$69.30
$99.00
OHu09679 NM_001267551.1 Homo sapiens phosphatidylethanolamine N-methyltransferase (PEMT), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $139.30
$199.00
OHu63981 XM_006721418.3 Homo sapiens phosphatidylethanolamine N-methyltransferase (PEMT), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $139.30
$199.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu10059
    Clone ID Related Accession (Same CDS sequence) NM_148172.2 , NM_148172.3
    Accession Version NM_148172.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 711bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-30
    Organism Homo sapiens(human)
    Product phosphatidylethanolamine N-methyltransferase isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC020558.4, AK302251.1, AF113126.1 and BC050593.1. On Nov 23, 2018 this sequence version replaced NM_148172.2. Transcript Variant: This variant (1) encodes the longest isoform (1). Isoform 1 localizes to the endoplasmic reticulum. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR5189655.125927.1, SRR5189655.107681.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA2145743 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology MANE Ensembl match :: ENST00000255389.10/ ENSP00000255389.5 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGAAGAGAT CTGGGAACCC GGGAGCCGAG GTAACGAACA GCTCGGTGGC AGGGCCTGAC 
    TGCTGCGGAG GCCTCGGCAA TATTGATTTT AGACAGGCAG ACTTCTGCGT TATGACCCGG
    CTGCTGGGCT ACGTGGACCC CCTGGATCCC AGCTTTGTGG CTGCCGTCAT CACCATCACC
    TTCAATCCGC TCTACTGGAA TGTGGTTGCA CGATGGGAAC ACAAGACCCG CAAGCTGAGC
    AGGGCCTTCG GATCCCCCTA CCTGGCCTGC TACTCTCTAA GCGTCACCAT CCTGCTCCTG
    AACTTCCTGC GCTCGCACTG CTTCACGCAG GCCATGCTGA GCCAGCCCAG GATGGAGAGC
    CTGGACACCC CCGCGGCCTA CAGCCTGGGC CTCGCGCTCC TGGGACTGGG CGTCGTGCTC
    GTGCTCTCCA GCTTCTTTGC ACTGGGGTTC GCTGGAACTT TCCTAGGTGA TTACTTCGGG
    ATCCTCAAGG AGGCGAGAGT GACCGTGTTC CCCTTCAACA TCCTGGACAA CCCCATGTAC
    TGGGGAAGCA CAGCCAACTA CCTGGGCTGG GCCATCATGC ACGCCAGCCC CACGGGCCTG
    CTCCTGACGG TGCTGGTGGC CCTCACCTAC ATAGTGGCTC TCCTATACGA AGAGCCCTTC
    ACCGCTGAGA TCTACCGGCA GAAAGCCTCC GGGTCCCACA AGAGGAGCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_680477.1
    CDS83..793
    Translation

    Target ORF information:

    RefSeq Version NM_148172.3
    Organism Homo sapiens(human)
    Definition Homo sapiens phosphatidylethanolamine N-methyltransferase (PEMT), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_148172.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGAAGAGAT CTGGGAACCC GGGAGCCGAG GTAACGAACA GCTCGGTGGC AGGGCCTGAC 
    TGCTGCGGAG GCCTCGGCAA TATTGATTTT AGACAGGCAG ACTTCTGCGT TATGACCCGG
    CTGCTGGGCT ACGTGGACCC CCTGGATCCC AGCTTTGTGG CTGCCGTCAT CACCATCACC
    TTCAATCCGC TCTACTGGAA TGTGGTTGCA CGATGGGAAC ACAAGACCCG CAAGCTGAGC
    AGGGCCTTCG GATCCCCCTA CCTGGCCTGC TACTCTCTAA GCGTCACCAT CCTGCTCCTG
    AACTTCCTGC GCTCGCACTG CTTCACGCAG GCCATGCTGA GCCAGCCCAG GATGGAGAGC
    CTGGACACCC CCGCGGCCTA CAGCCTGGGC CTCGCGCTCC TGGGACTGGG CGTCGTGCTC
    GTGCTCTCCA GCTTCTTTGC ACTGGGGTTC GCTGGAACTT TCCTAGGTGA TTACTTCGGG
    ATCCTCAAGG AGGCGAGAGT GACCGTGTTC CCCTTCAACA TCCTGGACAA CCCCATGTAC
    TGGGGAAGCA CAGCCAACTA CCTGGGCTGG GCCATCATGC ACGCCAGCCC CACGGGCCTG
    CTCCTGACGG TGCTGGTGGC CCTCACCTAC ATAGTGGCTC TCCTATACGA AGAGCCCTTC
    ACCGCTGAGA TCTACCGGCA GAAAGCCTCC GGGTCCCACA AGAGGAGCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu13080
    Clone ID Related Accession (Same CDS sequence) NM_007169.2 , NM_148173.1 , NM_148173.2 , NM_007169.3 , XM_024450532.1
    Accession Version NM_007169.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 600bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-22
    Organism Homo sapiens(human)
    Product phosphatidylethanolamine N-methyltransferase isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB029821.1. On Aug 29, 2002 this sequence version replaced NM_007169.1. Transcript Variant: This variant (2) differs in the 5' UTR and uses a downstream start codon compared to variant 1. Variants 2 and 3 encode the same isoform (2). Isoform 2 localizes to mitochondria-associated membranes. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB029821.1, AF176806.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2162895, SAMN04284274 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    ATGACCCGGC TGCTGGGCTA CGTGGACCCC CTGGATCCCA GCTTTGTGGC TGCCGTCATC 
    ACCATCACCT TCAATCCGCT CTACTGGAAT GTGGTTGCAC GATGGGAACA CAAGACCCGC
    AAGCTGAGCA GGGCCTTCGG ATCCCCCTAC CTGGCCTGCT ACTCTCTAAG CGTCACCATC
    CTGCTCCTGA ACTTCCTGCG CTCGCACTGC TTCACGCAGG CCATGCTGAG CCAGCCCAGG
    ATGGAGAGCC TGGACACCCC CGCGGCCTAC AGCCTGGGCC TCGCGCTCCT GGGACTGGGC
    GTCGTGCTCG TGCTCTCCAG CTTCTTTGCA CTGGGGTTCG CTGGAACTTT CCTAGGTGAT
    TACTTCGGGA TCCTCAAGGA GGCGAGAGTG ACCGTGTTCC CCTTCAACAT CCTGGACAAC
    CCCATGTACT GGGGAAGCAC AGCCAACTAC CTGGGCTGGG CCATCATGCA CGCCAGCCCC
    ACGGGCCTGC TCCTGACGGT GCTGGTGGCC CTCACCTACA TAGTGGCTCT CCTATACGAA
    GAGCCCTTCA CCGCTGAGAT CTACCGGCAG AAAGCCTCCG GGTCCCACAA GAGGAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_009100.2
    CDS181..780
    Translation

    Target ORF information:

    RefSeq Version NM_007169.2
    Organism Homo sapiens(human)
    Definition Homo sapiens phosphatidylethanolamine N-methyltransferase (PEMT), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_007169.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    ATGACCCGGC TGCTGGGCTA CGTGGACCCC CTGGATCCCA GCTTTGTGGC TGCCGTCATC 
    ACCATCACCT TCAATCCGCT CTACTGGAAT GTGGTTGCAC GATGGGAACA CAAGACCCGC
    AAGCTGAGCA GGGCCTTCGG ATCCCCCTAC CTGGCCTGCT ACTCTCTAAG CGTCACCATC
    CTGCTCCTGA ACTTCCTGCG CTCGCACTGC TTCACGCAGG CCATGCTGAG CCAGCCCAGG
    ATGGAGAGCC TGGACACCCC CGCGGCCTAC AGCCTGGGCC TCGCGCTCCT GGGACTGGGC
    GTCGTGCTCG TGCTCTCCAG CTTCTTTGCA CTGGGGTTCG CTGGAACTTT CCTAGGTGAT
    TACTTCGGGA TCCTCAAGGA GGCGAGAGTG ACCGTGTTCC CCTTCAACAT CCTGGACAAC
    CCCATGTACT GGGGAAGCAC AGCCAACTAC CTGGGCTGGG CCATCATGCA CGCCAGCCCC
    ACGGGCCTGC TCCTGACGGT GCTGGTGGCC CTCACCTACA TAGTGGCTCT CCTATACGAA
    GAGCCCTTCA CCGCTGAGAT CTACCGGCAG AAAGCCTCCG GGTCCCACAA GAGGAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu13080
    Clone ID Related Accession (Same CDS sequence) NM_007169.2 , NM_148173.1 , NM_148173.2 , NM_007169.3 , XM_024450532.1
    Accession Version NM_148173.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 600bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-22
    Organism Homo sapiens(human)
    Product phosphatidylethanolamine N-methyltransferase isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF176807.1. Transcript Variant: This variant (3) differs in the 5' UTR and uses a downstream start codon compared to variant 1. Variants 2 and 3 encode the same isoform (2). Isoform 2 localizes to mitochondria-associated membranes. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF176807.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2151119 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    ATGACCCGGC TGCTGGGCTA CGTGGACCCC CTGGATCCCA GCTTTGTGGC TGCCGTCATC 
    ACCATCACCT TCAATCCGCT CTACTGGAAT GTGGTTGCAC GATGGGAACA CAAGACCCGC
    AAGCTGAGCA GGGCCTTCGG ATCCCCCTAC CTGGCCTGCT ACTCTCTAAG CGTCACCATC
    CTGCTCCTGA ACTTCCTGCG CTCGCACTGC TTCACGCAGG CCATGCTGAG CCAGCCCAGG
    ATGGAGAGCC TGGACACCCC CGCGGCCTAC AGCCTGGGCC TCGCGCTCCT GGGACTGGGC
    GTCGTGCTCG TGCTCTCCAG CTTCTTTGCA CTGGGGTTCG CTGGAACTTT CCTAGGTGAT
    TACTTCGGGA TCCTCAAGGA GGCGAGAGTG ACCGTGTTCC CCTTCAACAT CCTGGACAAC
    CCCATGTACT GGGGAAGCAC AGCCAACTAC CTGGGCTGGG CCATCATGCA CGCCAGCCCC
    ACGGGCCTGC TCCTGACGGT GCTGGTGGCC CTCACCTACA TAGTGGCTCT CCTATACGAA
    GAGCCCTTCA CCGCTGAGAT CTACCGGCAG AAAGCCTCCG GGTCCCACAA GAGGAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_680478.1
    CDS68..667
    Translation

    Target ORF information:

    RefSeq Version NM_148173.1
    Organism Homo sapiens(human)
    Definition Homo sapiens phosphatidylethanolamine N-methyltransferase (PEMT), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_148173.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    ATGACCCGGC TGCTGGGCTA CGTGGACCCC CTGGATCCCA GCTTTGTGGC TGCCGTCATC 
    ACCATCACCT TCAATCCGCT CTACTGGAAT GTGGTTGCAC GATGGGAACA CAAGACCCGC
    AAGCTGAGCA GGGCCTTCGG ATCCCCCTAC CTGGCCTGCT ACTCTCTAAG CGTCACCATC
    CTGCTCCTGA ACTTCCTGCG CTCGCACTGC TTCACGCAGG CCATGCTGAG CCAGCCCAGG
    ATGGAGAGCC TGGACACCCC CGCGGCCTAC AGCCTGGGCC TCGCGCTCCT GGGACTGGGC
    GTCGTGCTCG TGCTCTCCAG CTTCTTTGCA CTGGGGTTCG CTGGAACTTT CCTAGGTGAT
    TACTTCGGGA TCCTCAAGGA GGCGAGAGTG ACCGTGTTCC CCTTCAACAT CCTGGACAAC
    CCCATGTACT GGGGAAGCAC AGCCAACTAC CTGGGCTGGG CCATCATGCA CGCCAGCCCC
    ACGGGCCTGC TCCTGACGGT GCTGGTGGCC CTCACCTACA TAGTGGCTCT CCTATACGAA
    GAGCCCTTCA CCGCTGAGAT CTACCGGCAG AAAGCCTCCG GGTCCCACAA GAGGAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu13080
    Clone ID Related Accession (Same CDS sequence) NM_007169.2 , NM_148173.1 , NM_148173.2 , NM_007169.3 , XM_024450532.1
    Accession Version NM_148173.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 600bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-08
    Organism Homo sapiens(human)
    Product phosphatidylethanolamine N-methyltransferase isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC020558.4. On Jun 2, 2019 this sequence version replaced NM_148173.1. Transcript Variant: This variant (3) differs in the 5' UTR and uses a downstream start codon compared to variant 1. Variants 2 and 3 encode the same isoform (2). Isoform 2 localizes to mitochondria-associated membranes. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF176807.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2151119 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    ATGACCCGGC TGCTGGGCTA CGTGGACCCC CTGGATCCCA GCTTTGTGGC TGCCGTCATC 
    ACCATCACCT TCAATCCGCT CTACTGGAAT GTGGTTGCAC GATGGGAACA CAAGACCCGC
    AAGCTGAGCA GGGCCTTCGG ATCCCCCTAC CTGGCCTGCT ACTCTCTAAG CGTCACCATC
    CTGCTCCTGA ACTTCCTGCG CTCGCACTGC TTCACGCAGG CCATGCTGAG CCAGCCCAGG
    ATGGAGAGCC TGGACACCCC CGCGGCCTAC AGCCTGGGCC TCGCGCTCCT GGGACTGGGC
    GTCGTGCTCG TGCTCTCCAG CTTCTTTGCA CTGGGGTTCG CTGGAACTTT CCTAGGTGAT
    TACTTCGGGA TCCTCAAGGA GGCGAGAGTG ACCGTGTTCC CCTTCAACAT CCTGGACAAC
    CCCATGTACT GGGGAAGCAC AGCCAACTAC CTGGGCTGGG CCATCATGCA CGCCAGCCCC
    ACGGGCCTGC TCCTGACGGT GCTGGTGGCC CTCACCTACA TAGTGGCTCT CCTATACGAA
    GAGCCCTTCA CCGCTGAGAT CTACCGGCAG AAAGCCTCCG GGTCCCACAA GAGGAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_680478.1
    CDS99..698
    Translation

    Target ORF information:

    RefSeq Version NM_148173.2
    Organism Homo sapiens(human)
    Definition Homo sapiens phosphatidylethanolamine N-methyltransferase (PEMT), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_148173.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    ATGACCCGGC TGCTGGGCTA CGTGGACCCC CTGGATCCCA GCTTTGTGGC TGCCGTCATC 
    ACCATCACCT TCAATCCGCT CTACTGGAAT GTGGTTGCAC GATGGGAACA CAAGACCCGC
    AAGCTGAGCA GGGCCTTCGG ATCCCCCTAC CTGGCCTGCT ACTCTCTAAG CGTCACCATC
    CTGCTCCTGA ACTTCCTGCG CTCGCACTGC TTCACGCAGG CCATGCTGAG CCAGCCCAGG
    ATGGAGAGCC TGGACACCCC CGCGGCCTAC AGCCTGGGCC TCGCGCTCCT GGGACTGGGC
    GTCGTGCTCG TGCTCTCCAG CTTCTTTGCA CTGGGGTTCG CTGGAACTTT CCTAGGTGAT
    TACTTCGGGA TCCTCAAGGA GGCGAGAGTG ACCGTGTTCC CCTTCAACAT CCTGGACAAC
    CCCATGTACT GGGGAAGCAC AGCCAACTAC CTGGGCTGGG CCATCATGCA CGCCAGCCCC
    ACGGGCCTGC TCCTGACGGT GCTGGTGGCC CTCACCTACA TAGTGGCTCT CCTATACGAA
    GAGCCCTTCA CCGCTGAGAT CTACCGGCAG AAAGCCTCCG GGTCCCACAA GAGGAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu13080
    Clone ID Related Accession (Same CDS sequence) NM_007169.2 , NM_148173.1 , NM_148173.2 , NM_007169.3 , XM_024450532.1
    Accession Version NM_007169.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 600bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Homo sapiens(human)
    Product phosphatidylethanolamine N-methyltransferase isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC020558.4. On Jun 2, 2019 this sequence version replaced NM_007169.2. Transcript Variant: This variant (2) differs in the 5' UTR and uses a downstream start codon compared to variant 1. Variants 2 and 3 encode the same isoform (2). Isoform 2 localizes to mitochondria-associated membranes. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR5189664.45900.1, AF176806.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2162895, SAMN04284274 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    ATGACCCGGC TGCTGGGCTA CGTGGACCCC CTGGATCCCA GCTTTGTGGC TGCCGTCATC 
    ACCATCACCT TCAATCCGCT CTACTGGAAT GTGGTTGCAC GATGGGAACA CAAGACCCGC
    AAGCTGAGCA GGGCCTTCGG ATCCCCCTAC CTGGCCTGCT ACTCTCTAAG CGTCACCATC
    CTGCTCCTGA ACTTCCTGCG CTCGCACTGC TTCACGCAGG CCATGCTGAG CCAGCCCAGG
    ATGGAGAGCC TGGACACCCC CGCGGCCTAC AGCCTGGGCC TCGCGCTCCT GGGACTGGGC
    GTCGTGCTCG TGCTCTCCAG CTTCTTTGCA CTGGGGTTCG CTGGAACTTT CCTAGGTGAT
    TACTTCGGGA TCCTCAAGGA GGCGAGAGTG ACCGTGTTCC CCTTCAACAT CCTGGACAAC
    CCCATGTACT GGGGAAGCAC AGCCAACTAC CTGGGCTGGG CCATCATGCA CGCCAGCCCC
    ACGGGCCTGC TCCTGACGGT GCTGGTGGCC CTCACCTACA TAGTGGCTCT CCTATACGAA
    GAGCCCTTCA CCGCTGAGAT CTACCGGCAG AAAGCCTCCG GGTCCCACAA GAGGAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_009100.2
    CDS196..795
    Translation

    Target ORF information:

    RefSeq Version NM_007169.3
    Organism Homo sapiens(human)
    Definition Homo sapiens phosphatidylethanolamine N-methyltransferase (PEMT), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_007169.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    ATGACCCGGC TGCTGGGCTA CGTGGACCCC CTGGATCCCA GCTTTGTGGC TGCCGTCATC 
    ACCATCACCT TCAATCCGCT CTACTGGAAT GTGGTTGCAC GATGGGAACA CAAGACCCGC
    AAGCTGAGCA GGGCCTTCGG ATCCCCCTAC CTGGCCTGCT ACTCTCTAAG CGTCACCATC
    CTGCTCCTGA ACTTCCTGCG CTCGCACTGC TTCACGCAGG CCATGCTGAG CCAGCCCAGG
    ATGGAGAGCC TGGACACCCC CGCGGCCTAC AGCCTGGGCC TCGCGCTCCT GGGACTGGGC
    GTCGTGCTCG TGCTCTCCAG CTTCTTTGCA CTGGGGTTCG CTGGAACTTT CCTAGGTGAT
    TACTTCGGGA TCCTCAAGGA GGCGAGAGTG ACCGTGTTCC CCTTCAACAT CCTGGACAAC
    CCCATGTACT GGGGAAGCAC AGCCAACTAC CTGGGCTGGG CCATCATGCA CGCCAGCCCC
    ACGGGCCTGC TCCTGACGGT GCTGGTGGCC CTCACCTACA TAGTGGCTCT CCTATACGAA
    GAGCCCTTCA CCGCTGAGAT CTACCGGCAG AAAGCCTCCG GGTCCCACAA GAGGAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu13080
    Clone ID Related Accession (Same CDS sequence) NM_007169.2 , NM_148173.1 , NM_148173.2 , NM_007169.3 , XM_024450532.1
    Accession Version XM_024450532.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 600bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product phosphatidylethanolamine N-methyltransferase isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    ATGACCCGGC TGCTGGGCTA CGTGGACCCC CTGGATCCCA GCTTTGTGGC TGCCGTCATC 
    ACCATCACCT TCAATCCGCT CTACTGGAAT GTGGTTGCAC GATGGGAACA CAAGACCCGC
    AAGCTGAGCA GGGCCTTCGG ATCCCCCTAC CTGGCCTGCT ACTCTCTAAG CGTCACCATC
    CTGCTCCTGA ACTTCCTGCG CTCGCACTGC TTCACGCAGG CCATGCTGAG CCAGCCCAGG
    ATGGAGAGCC TGGACACCCC CGCGGCCTAC AGCCTGGGCC TCGCGCTCCT GGGACTGGGC
    GTCGTGCTCG TGCTCTCCAG CTTCTTTGCA CTGGGGTTCG CTGGAACTTT CCTAGGTGAT
    TACTTCGGGA TCCTCAAGGA GGCGAGAGTG ACCGTGTTCC CCTTCAACAT CCTGGACAAC
    CCCATGTACT GGGGAAGCAC AGCCAACTAC CTGGGCTGGG CCATCATGCA CGCCAGCCCC
    ACGGGCCTGC TCCTGACGGT GCTGGTGGCC CTCACCTACA TAGTGGCTCT CCTATACGAA
    GAGCCCTTCA CCGCTGAGAT CTACCGGCAG AAAGCCTCCG GGTCCCACAA GAGGAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024306300.1
    CDS37..636
    Translation

    Target ORF information:

    RefSeq Version XM_024450532.1
    Organism Homo sapiens(human)
    Definition Homo sapiens phosphatidylethanolamine N-methyltransferase (PEMT), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024450532.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    ATGACCCGGC TGCTGGGCTA CGTGGACCCC CTGGATCCCA GCTTTGTGGC TGCCGTCATC 
    ACCATCACCT TCAATCCGCT CTACTGGAAT GTGGTTGCAC GATGGGAACA CAAGACCCGC
    AAGCTGAGCA GGGCCTTCGG ATCCCCCTAC CTGGCCTGCT ACTCTCTAAG CGTCACCATC
    CTGCTCCTGA ACTTCCTGCG CTCGCACTGC TTCACGCAGG CCATGCTGAG CCAGCCCAGG
    ATGGAGAGCC TGGACACCCC CGCGGCCTAC AGCCTGGGCC TCGCGCTCCT GGGACTGGGC
    GTCGTGCTCG TGCTCTCCAG CTTCTTTGCA CTGGGGTTCG CTGGAACTTT CCTAGGTGAT
    TACTTCGGGA TCCTCAAGGA GGCGAGAGTG ACCGTGTTCC CCTTCAACAT CCTGGACAAC
    CCCATGTACT GGGGAAGCAC AGCCAACTAC CTGGGCTGGG CCATCATGCA CGCCAGCCCC
    ACGGGCCTGC TCCTGACGGT GCTGGTGGCC CTCACCTACA TAGTGGCTCT CCTATACGAA
    GAGCCCTTCA CCGCTGAGAT CTACCGGCAG AAAGCCTCCG GGTCCCACAA GAGGAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu09624
    Clone ID Related Accession (Same CDS sequence) NM_001267552.1 , NM_001267552.2
    Accession Version NM_001267552.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 699bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-24
    Organism Homo sapiens(human)
    Product phosphatidylethanolamine N-methyltransferase isoform 3
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK302251.1 and BC050593.1. Transcript Variant: This variant (5) has an additional exon in the 3' coding region, compared to variant 1, which results in a frameshift and a protein (isoform 3) with a shorter and distinct C-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK302251.1, CX781049.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGAAGAGAT CTGGGAACCC GGGAGCCGAG GTAACGAACA GCTCGGTGGC AGGGCCTGAC 
    TGCTGCGGAG GCCTCGGCAA TATTGATTTT AGACAGGCAG ACTTCTGCGT TATGACCCGG
    CTGCTGGGCT ACGTGGACCC CCTGGATCCC AGCTTTGTGG CTGCCGTCAT CACCATCACC
    TTCAATCCGC TCTACTGGAA TGTGGTTGCA CGATGGGAAC ACAAGACCCG CAAGCTGAGC
    AGGGCCTTCG GATCCCCCTA CCTGGCCTGC TACTCTCTAA GCGTCACCAT CCTGCTCCTG
    AACTTCCTGC GCTCGCACTG CTTCACGCAG GCCATGCTGA GCCAGCCCAG GATGGAGAGC
    CTGGACACCC CCGCGGCCTA CAGCCTGGGC CTCGCGCTCC TGGGACTGGG CGTCGTGCTC
    GTGCTCTCCA GCTTCTTTGC ACTGGGGTTC GCTGGAACTT TCCTAGGTGA TTACTTCGGG
    ATCCTCAAGG AGGCGAGAGT GACCGTGTTC CCCTTCAACA TCCTGGACAA CCCCATGTAC
    TGGGGAAGCA CAGCCAACTA CCTGGGCTGG GCCATCATCC CAGCACCTGC GGGAGCTGTG
    GGATCAGAGG CACGCCAGCC CCACGGGCCT GCTCCTGACG GTGCTGGTGG CCCTCACCTA
    CATAGTGGCT CTCCTATACG AAGAGCCCTT CACCGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001254481.1
    CDS78..776
    Translation

    Target ORF information:

    RefSeq Version NM_001267552.1
    Organism Homo sapiens(human)
    Definition Homo sapiens phosphatidylethanolamine N-methyltransferase (PEMT), transcript variant 5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001267552.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGAAGAGAT CTGGGAACCC GGGAGCCGAG GTAACGAACA GCTCGGTGGC AGGGCCTGAC 
    TGCTGCGGAG GCCTCGGCAA TATTGATTTT AGACAGGCAG ACTTCTGCGT TATGACCCGG
    CTGCTGGGCT ACGTGGACCC CCTGGATCCC AGCTTTGTGG CTGCCGTCAT CACCATCACC
    TTCAATCCGC TCTACTGGAA TGTGGTTGCA CGATGGGAAC ACAAGACCCG CAAGCTGAGC
    AGGGCCTTCG GATCCCCCTA CCTGGCCTGC TACTCTCTAA GCGTCACCAT CCTGCTCCTG
    AACTTCCTGC GCTCGCACTG CTTCACGCAG GCCATGCTGA GCCAGCCCAG GATGGAGAGC
    CTGGACACCC CCGCGGCCTA CAGCCTGGGC CTCGCGCTCC TGGGACTGGG CGTCGTGCTC
    GTGCTCTCCA GCTTCTTTGC ACTGGGGTTC GCTGGAACTT TCCTAGGTGA TTACTTCGGG
    ATCCTCAAGG AGGCGAGAGT GACCGTGTTC CCCTTCAACA TCCTGGACAA CCCCATGTAC
    TGGGGAAGCA CAGCCAACTA CCTGGGCTGG GCCATCATCC CAGCACCTGC GGGAGCTGTG
    GGATCAGAGG CACGCCAGCC CCACGGGCCT GCTCCTGACG GTGCTGGTGG CCCTCACCTA
    CATAGTGGCT CTCCTATACG AAGAGCCCTT CACCGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu09624
    Clone ID Related Accession (Same CDS sequence) NM_001267552.1 , NM_001267552.2
    Accession Version NM_001267552.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 699bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-06
    Organism Homo sapiens(human)
    Product phosphatidylethanolamine N-methyltransferase isoform 3
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC020558.4, AK302251.1 and BC050593.1. On May 31, 2019 this sequence version replaced NM_001267552.1. Transcript Variant: This variant (5) has an additional exon in the 3' coding region, compared to variant 1, which results in a frameshift and a protein (isoform 3) with a shorter and distinct C-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK302251.1, CX781049.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGAAGAGAT CTGGGAACCC GGGAGCCGAG GTAACGAACA GCTCGGTGGC AGGGCCTGAC 
    TGCTGCGGAG GCCTCGGCAA TATTGATTTT AGACAGGCAG ACTTCTGCGT TATGACCCGG
    CTGCTGGGCT ACGTGGACCC CCTGGATCCC AGCTTTGTGG CTGCCGTCAT CACCATCACC
    TTCAATCCGC TCTACTGGAA TGTGGTTGCA CGATGGGAAC ACAAGACCCG CAAGCTGAGC
    AGGGCCTTCG GATCCCCCTA CCTGGCCTGC TACTCTCTAA GCGTCACCAT CCTGCTCCTG
    AACTTCCTGC GCTCGCACTG CTTCACGCAG GCCATGCTGA GCCAGCCCAG GATGGAGAGC
    CTGGACACCC CCGCGGCCTA CAGCCTGGGC CTCGCGCTCC TGGGACTGGG CGTCGTGCTC
    GTGCTCTCCA GCTTCTTTGC ACTGGGGTTC GCTGGAACTT TCCTAGGTGA TTACTTCGGG
    ATCCTCAAGG AGGCGAGAGT GACCGTGTTC CCCTTCAACA TCCTGGACAA CCCCATGTAC
    TGGGGAAGCA CAGCCAACTA CCTGGGCTGG GCCATCATCC CAGCACCTGC GGGAGCTGTG
    GGATCAGAGG CACGCCAGCC CCACGGGCCT GCTCCTGACG GTGCTGGTGG CCCTCACCTA
    CATAGTGGCT CTCCTATACG AAGAGCCCTT CACCGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001254481.1
    CDS83..781
    Translation

    Target ORF information:

    RefSeq Version NM_001267552.2
    Organism Homo sapiens(human)
    Definition Homo sapiens phosphatidylethanolamine N-methyltransferase (PEMT), transcript variant 5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001267552.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGAAGAGAT CTGGGAACCC GGGAGCCGAG GTAACGAACA GCTCGGTGGC AGGGCCTGAC 
    TGCTGCGGAG GCCTCGGCAA TATTGATTTT AGACAGGCAG ACTTCTGCGT TATGACCCGG
    CTGCTGGGCT ACGTGGACCC CCTGGATCCC AGCTTTGTGG CTGCCGTCAT CACCATCACC
    TTCAATCCGC TCTACTGGAA TGTGGTTGCA CGATGGGAAC ACAAGACCCG CAAGCTGAGC
    AGGGCCTTCG GATCCCCCTA CCTGGCCTGC TACTCTCTAA GCGTCACCAT CCTGCTCCTG
    AACTTCCTGC GCTCGCACTG CTTCACGCAG GCCATGCTGA GCCAGCCCAG GATGGAGAGC
    CTGGACACCC CCGCGGCCTA CAGCCTGGGC CTCGCGCTCC TGGGACTGGG CGTCGTGCTC
    GTGCTCTCCA GCTTCTTTGC ACTGGGGTTC GCTGGAACTT TCCTAGGTGA TTACTTCGGG
    ATCCTCAAGG AGGCGAGAGT GACCGTGTTC CCCTTCAACA TCCTGGACAA CCCCATGTAC
    TGGGGAAGCA CAGCCAACTA CCTGGGCTGG GCCATCATCC CAGCACCTGC GGGAGCTGTG
    GGATCAGAGG CACGCCAGCC CCACGGGCCT GCTCCTGACG GTGCTGGTGG CCCTCACCTA
    CATAGTGGCT CTCCTATACG AAGAGCCCTT CACCGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu09679
    Clone ID Related Accession (Same CDS sequence) NM_001267551.1 , NM_001267551.2
    Accession Version NM_001267551.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 645bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-24
    Organism Homo sapiens(human)
    Product phosphatidylethanolamine N-methyltransferase isoform 4
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK302251.1, BM011377.1 and BC050593.1. Transcript Variant: This variant (4) uses an alternate splice site in the 5' coding region compared to variant 1. The resulting protein (isoform 4) is shorter but has the same N- and C-termini compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: ERR279846.1258.1, SRR1163658.196059.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743, SAMEA2153946 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGAAGAGAT CTGGGAACCC GGGAGCCGAG GCAGACTTCT GCGTTATGAC CCGGCTGCTG 
    GGCTACGTGG ACCCCCTGGA TCCCAGCTTT GTGGCTGCCG TCATCACCAT CACCTTCAAT
    CCGCTCTACT GGAATGTGGT TGCACGATGG GAACACAAGA CCCGCAAGCT GAGCAGGGCC
    TTCGGATCCC CCTACCTGGC CTGCTACTCT CTAAGCGTCA CCATCCTGCT CCTGAACTTC
    CTGCGCTCGC ACTGCTTCAC GCAGGCCATG CTGAGCCAGC CCAGGATGGA GAGCCTGGAC
    ACCCCCGCGG CCTACAGCCT GGGCCTCGCG CTCCTGGGAC TGGGCGTCGT GCTCGTGCTC
    TCCAGCTTCT TTGCACTGGG GTTCGCTGGA ACTTTCCTAG GTGATTACTT CGGGATCCTC
    AAGGAGGCGA GAGTGACCGT GTTCCCCTTC AACATCCTGG ACAACCCCAT GTACTGGGGA
    AGCACAGCCA ACTACCTGGG CTGGGCCATC ATGCACGCCA GCCCCACGGG CCTGCTCCTG
    ACGGTGCTGG TGGCCCTCAC CTACATAGTG GCTCTCCTAT ACGAAGAGCC CTTCACCGCT
    GAGATCTACC GGCAGAAAGC CTCCGGGTCC CACAAGAGGA GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001254480.1
    CDS78..722
    Translation

    Target ORF information:

    RefSeq Version NM_001267551.1
    Organism Homo sapiens(human)
    Definition Homo sapiens phosphatidylethanolamine N-methyltransferase (PEMT), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001267551.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGAAGAGAT CTGGGAACCC GGGAGCCGAG GCAGACTTCT GCGTTATGAC CCGGCTGCTG 
    GGCTACGTGG ACCCCCTGGA TCCCAGCTTT GTGGCTGCCG TCATCACCAT CACCTTCAAT
    CCGCTCTACT GGAATGTGGT TGCACGATGG GAACACAAGA CCCGCAAGCT GAGCAGGGCC
    TTCGGATCCC CCTACCTGGC CTGCTACTCT CTAAGCGTCA CCATCCTGCT CCTGAACTTC
    CTGCGCTCGC ACTGCTTCAC GCAGGCCATG CTGAGCCAGC CCAGGATGGA GAGCCTGGAC
    ACCCCCGCGG CCTACAGCCT GGGCCTCGCG CTCCTGGGAC TGGGCGTCGT GCTCGTGCTC
    TCCAGCTTCT TTGCACTGGG GTTCGCTGGA ACTTTCCTAG GTGATTACTT CGGGATCCTC
    AAGGAGGCGA GAGTGACCGT GTTCCCCTTC AACATCCTGG ACAACCCCAT GTACTGGGGA
    AGCACAGCCA ACTACCTGGG CTGGGCCATC ATGCACGCCA GCCCCACGGG CCTGCTCCTG
    ACGGTGCTGG TGGCCCTCAC CTACATAGTG GCTCTCCTAT ACGAAGAGCC CTTCACCGCT
    GAGATCTACC GGCAGAAAGC CTCCGGGTCC CACAAGAGGA GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu09679
    Clone ID Related Accession (Same CDS sequence) NM_001267551.1 , NM_001267551.2
    Accession Version NM_001267551.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 645bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Homo sapiens(human)
    Product phosphatidylethanolamine N-methyltransferase isoform 4
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC020558.4, AK302251.1, BM011377.1 and BC050593.1. On May 31, 2019 this sequence version replaced NM_001267551.1. Transcript Variant: This variant (4) uses an alternate splice site in the 5' coding region compared to variant 1. The resulting protein (isoform 4) is shorter but has the same N- and C-termini compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: ERR279846.1258.1, DRR138522.1106519.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743, SAMEA2153946 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGAAGAGAT CTGGGAACCC GGGAGCCGAG GCAGACTTCT GCGTTATGAC CCGGCTGCTG 
    GGCTACGTGG ACCCCCTGGA TCCCAGCTTT GTGGCTGCCG TCATCACCAT CACCTTCAAT
    CCGCTCTACT GGAATGTGGT TGCACGATGG GAACACAAGA CCCGCAAGCT GAGCAGGGCC
    TTCGGATCCC CCTACCTGGC CTGCTACTCT CTAAGCGTCA CCATCCTGCT CCTGAACTTC
    CTGCGCTCGC ACTGCTTCAC GCAGGCCATG CTGAGCCAGC CCAGGATGGA GAGCCTGGAC
    ACCCCCGCGG CCTACAGCCT GGGCCTCGCG CTCCTGGGAC TGGGCGTCGT GCTCGTGCTC
    TCCAGCTTCT TTGCACTGGG GTTCGCTGGA ACTTTCCTAG GTGATTACTT CGGGATCCTC
    AAGGAGGCGA GAGTGACCGT GTTCCCCTTC AACATCCTGG ACAACCCCAT GTACTGGGGA
    AGCACAGCCA ACTACCTGGG CTGGGCCATC ATGCACGCCA GCCCCACGGG CCTGCTCCTG
    ACGGTGCTGG TGGCCCTCAC CTACATAGTG GCTCTCCTAT ACGAAGAGCC CTTCACCGCT
    GAGATCTACC GGCAGAAAGC CTCCGGGTCC CACAAGAGGA GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001254480.1
    CDS83..727
    Translation

    Target ORF information:

    RefSeq Version NM_001267551.2
    Organism Homo sapiens(human)
    Definition Homo sapiens phosphatidylethanolamine N-methyltransferase (PEMT), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001267551.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGAAGAGAT CTGGGAACCC GGGAGCCGAG GCAGACTTCT GCGTTATGAC CCGGCTGCTG 
    GGCTACGTGG ACCCCCTGGA TCCCAGCTTT GTGGCTGCCG TCATCACCAT CACCTTCAAT
    CCGCTCTACT GGAATGTGGT TGCACGATGG GAACACAAGA CCCGCAAGCT GAGCAGGGCC
    TTCGGATCCC CCTACCTGGC CTGCTACTCT CTAAGCGTCA CCATCCTGCT CCTGAACTTC
    CTGCGCTCGC ACTGCTTCAC GCAGGCCATG CTGAGCCAGC CCAGGATGGA GAGCCTGGAC
    ACCCCCGCGG CCTACAGCCT GGGCCTCGCG CTCCTGGGAC TGGGCGTCGT GCTCGTGCTC
    TCCAGCTTCT TTGCACTGGG GTTCGCTGGA ACTTTCCTAG GTGATTACTT CGGGATCCTC
    AAGGAGGCGA GAGTGACCGT GTTCCCCTTC AACATCCTGG ACAACCCCAT GTACTGGGGA
    AGCACAGCCA ACTACCTGGG CTGGGCCATC ATGCACGCCA GCCCCACGGG CCTGCTCCTG
    ACGGTGCTGG TGGCCCTCAC CTACATAGTG GCTCTCCTAT ACGAAGAGCC CTTCACCGCT
    GAGATCTACC GGCAGAAAGC CTCCGGGTCC CACAAGAGGA GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu10059
    Clone ID Related Accession (Same CDS sequence) NM_148172.2 , NM_148172.3
    Accession Version NM_148172.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 711bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-01
    Organism Homo sapiens(human)
    Product phosphatidylethanolamine N-methyltransferase isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK302251.1, AF113126.1 and BC050593.1. On May 31, 2012 this sequence version replaced NM_148172.1. Transcript Variant: This variant (1) encodes the longest isoform (1). Isoform 1 localizes to the endoplasmic reticulum. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC050593.1, SRR1163657.403557.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA2145743 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGAAGAGAT CTGGGAACCC GGGAGCCGAG GTAACGAACA GCTCGGTGGC AGGGCCTGAC 
    TGCTGCGGAG GCCTCGGCAA TATTGATTTT AGACAGGCAG ACTTCTGCGT TATGACCCGG
    CTGCTGGGCT ACGTGGACCC CCTGGATCCC AGCTTTGTGG CTGCCGTCAT CACCATCACC
    TTCAATCCGC TCTACTGGAA TGTGGTTGCA CGATGGGAAC ACAAGACCCG CAAGCTGAGC
    AGGGCCTTCG GATCCCCCTA CCTGGCCTGC TACTCTCTAA GCGTCACCAT CCTGCTCCTG
    AACTTCCTGC GCTCGCACTG CTTCACGCAG GCCATGCTGA GCCAGCCCAG GATGGAGAGC
    CTGGACACCC CCGCGGCCTA CAGCCTGGGC CTCGCGCTCC TGGGACTGGG CGTCGTGCTC
    GTGCTCTCCA GCTTCTTTGC ACTGGGGTTC GCTGGAACTT TCCTAGGTGA TTACTTCGGG
    ATCCTCAAGG AGGCGAGAGT GACCGTGTTC CCCTTCAACA TCCTGGACAA CCCCATGTAC
    TGGGGAAGCA CAGCCAACTA CCTGGGCTGG GCCATCATGC ACGCCAGCCC CACGGGCCTG
    CTCCTGACGG TGCTGGTGGC CCTCACCTAC ATAGTGGCTC TCCTATACGA AGAGCCCTTC
    ACCGCTGAGA TCTACCGGCA GAAAGCCTCC GGGTCCCACA AGAGGAGCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_680477.1
    CDS78..788
    Translation

    Target ORF information:

    RefSeq Version NM_148172.2
    Organism Homo sapiens(human)
    Definition Homo sapiens phosphatidylethanolamine N-methyltransferase (PEMT), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_148172.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGAAGAGAT CTGGGAACCC GGGAGCCGAG GTAACGAACA GCTCGGTGGC AGGGCCTGAC 
    TGCTGCGGAG GCCTCGGCAA TATTGATTTT AGACAGGCAG ACTTCTGCGT TATGACCCGG
    CTGCTGGGCT ACGTGGACCC CCTGGATCCC AGCTTTGTGG CTGCCGTCAT CACCATCACC
    TTCAATCCGC TCTACTGGAA TGTGGTTGCA CGATGGGAAC ACAAGACCCG CAAGCTGAGC
    AGGGCCTTCG GATCCCCCTA CCTGGCCTGC TACTCTCTAA GCGTCACCAT CCTGCTCCTG
    AACTTCCTGC GCTCGCACTG CTTCACGCAG GCCATGCTGA GCCAGCCCAG GATGGAGAGC
    CTGGACACCC CCGCGGCCTA CAGCCTGGGC CTCGCGCTCC TGGGACTGGG CGTCGTGCTC
    GTGCTCTCCA GCTTCTTTGC ACTGGGGTTC GCTGGAACTT TCCTAGGTGA TTACTTCGGG
    ATCCTCAAGG AGGCGAGAGT GACCGTGTTC CCCTTCAACA TCCTGGACAA CCCCATGTAC
    TGGGGAAGCA CAGCCAACTA CCTGGGCTGG GCCATCATGC ACGCCAGCCC CACGGGCCTG
    CTCCTGACGG TGCTGGTGGC CCTCACCTAC ATAGTGGCTC TCCTATACGA AGAGCCCTTC
    ACCGCTGAGA TCTACCGGCA GAAAGCCTCC GGGTCCCACA AGAGGAGCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu63981
    Clone ID Related Accession (Same CDS sequence) XM_006721418.3 , XM_006721418.4
    Accession Version XM_006721418.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 648bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product phosphatidylethanolamine N-methyltransferase isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006721418.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGTTCCCAG GCCTCCCAGT GTGTTCCCAG AGGGCAGACT TCTGCGTTAT GACCCGGCTG 
    CTGGGCTACG TGGACCCCCT GGATCCCAGC TTTGTGGCTG CCGTCATCAC CATCACCTTC
    AATCCGCTCT ACTGGAATGT GGTTGCACGA TGGGAACACA AGACCCGCAA GCTGAGCAGG
    GCCTTCGGAT CCCCCTACCT GGCCTGCTAC TCTCTAAGCG TCACCATCCT GCTCCTGAAC
    TTCCTGCGCT CGCACTGCTT CACGCAGGCC ATGCTGAGCC AGCCCAGGAT GGAGAGCCTG
    GACACCCCCG CGGCCTACAG CCTGGGCCTC GCGCTCCTGG GACTGGGCGT CGTGCTCGTG
    CTCTCCAGCT TCTTTGCACT GGGGTTCGCT GGAACTTTCC TAGGTGATTA CTTCGGGATC
    CTCAAGGAGG CGAGAGTGAC CGTGTTCCCC TTCAACATCC TGGACAACCC CATGTACTGG
    GGAAGCACAG CCAACTACCT GGGCTGGGCC ATCATGCACG CCAGCCCCAC GGGCCTGCTC
    CTGACGGTGC TGGTGGCCCT CACCTACATA GTGGCTCTCC TATACGAAGA GCCCTTCACC
    GCTGAGATCT ACCGGCAGAA AGCCTCCGGG TCCCACAAGA GGAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006721481.2
    CDS22..669
    Translation

    Target ORF information:

    RefSeq Version XM_006721418.3
    Organism Homo sapiens(human)
    Definition Homo sapiens phosphatidylethanolamine N-methyltransferase (PEMT), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006721418.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGTTCCCAG GCCTCCCAGT GTGTTCCCAG AGGGCAGACT TCTGCGTTAT GACCCGGCTG 
    CTGGGCTACG TGGACCCCCT GGATCCCAGC TTTGTGGCTG CCGTCATCAC CATCACCTTC
    AATCCGCTCT ACTGGAATGT GGTTGCACGA TGGGAACACA AGACCCGCAA GCTGAGCAGG
    GCCTTCGGAT CCCCCTACCT GGCCTGCTAC TCTCTAAGCG TCACCATCCT GCTCCTGAAC
    TTCCTGCGCT CGCACTGCTT CACGCAGGCC ATGCTGAGCC AGCCCAGGAT GGAGAGCCTG
    GACACCCCCG CGGCCTACAG CCTGGGCCTC GCGCTCCTGG GACTGGGCGT CGTGCTCGTG
    CTCTCCAGCT TCTTTGCACT GGGGTTCGCT GGAACTTTCC TAGGTGATTA CTTCGGGATC
    CTCAAGGAGG CGAGAGTGAC CGTGTTCCCC TTCAACATCC TGGACAACCC CATGTACTGG
    GGAAGCACAG CCAACTACCT GGGCTGGGCC ATCATGCACG CCAGCCCCAC GGGCCTGCTC
    CTGACGGTGC TGGTGGCCCT CACCTACATA GTGGCTCTCC TATACGAAGA GCCCTTCACC
    GCTGAGATCT ACCGGCAGAA AGCCTCCGGG TCCCACAAGA GGAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu63981
    Clone ID Related Accession (Same CDS sequence) XM_006721418.3 , XM_006721418.4
    Accession Version XM_006721418.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 648bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product phosphatidylethanolamine N-methyltransferase isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_006721418.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGTTCCCAG GCCTCCCAGT GTGTTCCCAG AGGGCAGACT TCTGCGTTAT GACCCGGCTG 
    CTGGGCTACG TGGACCCCCT GGATCCCAGC TTTGTGGCTG CCGTCATCAC CATCACCTTC
    AATCCGCTCT ACTGGAATGT GGTTGCACGA TGGGAACACA AGACCCGCAA GCTGAGCAGG
    GCCTTCGGAT CCCCCTACCT GGCCTGCTAC TCTCTAAGCG TCACCATCCT GCTCCTGAAC
    TTCCTGCGCT CGCACTGCTT CACGCAGGCC ATGCTGAGCC AGCCCAGGAT GGAGAGCCTG
    GACACCCCCG CGGCCTACAG CCTGGGCCTC GCGCTCCTGG GACTGGGCGT CGTGCTCGTG
    CTCTCCAGCT TCTTTGCACT GGGGTTCGCT GGAACTTTCC TAGGTGATTA CTTCGGGATC
    CTCAAGGAGG CGAGAGTGAC CGTGTTCCCC TTCAACATCC TGGACAACCC CATGTACTGG
    GGAAGCACAG CCAACTACCT GGGCTGGGCC ATCATGCACG CCAGCCCCAC GGGCCTGCTC
    CTGACGGTGC TGGTGGCCCT CACCTACATA GTGGCTCTCC TATACGAAGA GCCCTTCACC
    GCTGAGATCT ACCGGCAGAA AGCCTCCGGG TCCCACAAGA GGAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006721481.2
    CDS27..674
    Translation

    Target ORF information:

    RefSeq Version XM_006721418.4
    Organism Homo sapiens(human)
    Definition Homo sapiens phosphatidylethanolamine N-methyltransferase (PEMT), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006721418.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGTTCCCAG GCCTCCCAGT GTGTTCCCAG AGGGCAGACT TCTGCGTTAT GACCCGGCTG 
    CTGGGCTACG TGGACCCCCT GGATCCCAGC TTTGTGGCTG CCGTCATCAC CATCACCTTC
    AATCCGCTCT ACTGGAATGT GGTTGCACGA TGGGAACACA AGACCCGCAA GCTGAGCAGG
    GCCTTCGGAT CCCCCTACCT GGCCTGCTAC TCTCTAAGCG TCACCATCCT GCTCCTGAAC
    TTCCTGCGCT CGCACTGCTT CACGCAGGCC ATGCTGAGCC AGCCCAGGAT GGAGAGCCTG
    GACACCCCCG CGGCCTACAG CCTGGGCCTC GCGCTCCTGG GACTGGGCGT CGTGCTCGTG
    CTCTCCAGCT TCTTTGCACT GGGGTTCGCT GGAACTTTCC TAGGTGATTA CTTCGGGATC
    CTCAAGGAGG CGAGAGTGAC CGTGTTCCCC TTCAACATCC TGGACAACCC CATGTACTGG
    GGAAGCACAG CCAACTACCT GGGCTGGGCC ATCATGCACG CCAGCCCCAC GGGCCTGCTC
    CTGACGGTGC TGGTGGCCCT CACCTACATA GTGGCTCTCC TATACGAAGA GCCCTTCACC
    GCTGAGATCT ACCGGCAGAA AGCCTCCGGG TCCCACAAGA GGAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu86479
    Clone ID Related Accession (Same CDS sequence) XM_017024016.1
    Accession Version XM_017024016.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 378bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product phosphatidylethanolamine N-methyltransferase isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    ATGCTGAGCC AGCCCAGGAT GGAGAGCCTG GACACCCCCG CGGCCTACAG CCTGGGCCTC 
    GCGCTCCTGG GACTGGGCGT CGTGCTCGTG CTCTCCAGCT TCTTTGCACT GGGGTTCGCT
    GGAACTTTCC TAGGTGATTA CTTCGGGATC CTCAAGGAGG CGAGAGTGAC CGTGTTCCCC
    TTCAACATCC TGGACAACCC CATGTACTGG GGAAGCACAG CCAACTACCT GGGCTGGGCC
    ATCATGCACG CCAGCCCCAC GGGCCTGCTC CTGACGGTGC TGGTGGCCCT CACCTACATA
    GTGGCTCTCC TATACGAAGA GCCCTTCACC GCTGAGATCT ACCGGCAGAA AGCCTCCGGG
    TCCCACAAGA GGAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016879505.1
    CDS198..575
    Translation

    Target ORF information:

    RefSeq Version XM_017024016.1
    Organism Homo sapiens(human)
    Definition Homo sapiens phosphatidylethanolamine N-methyltransferase (PEMT), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017024016.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    ATGCTGAGCC AGCCCAGGAT GGAGAGCCTG GACACCCCCG CGGCCTACAG CCTGGGCCTC 
    GCGCTCCTGG GACTGGGCGT CGTGCTCGTG CTCTCCAGCT TCTTTGCACT GGGGTTCGCT
    GGAACTTTCC TAGGTGATTA CTTCGGGATC CTCAAGGAGG CGAGAGTGAC CGTGTTCCCC
    TTCAACATCC TGGACAACCC CATGTACTGG GGAAGCACAG CCAACTACCT GGGCTGGGCC
    ATCATGCACG CCAGCCCCAC GGGCCTGCTC CTGACGGTGC TGGTGGCCCT CACCTACATA
    GTGGCTCTCC TATACGAAGA GCCCTTCACC GCTGAGATCT ACCGGCAGAA AGCCTCCGGG
    TCCCACAAGA GGAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.