SF3A1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	SF3A1
Entrez Gene ID	10291
Full Name	splicing factor 3a subunit 1
Synonyms	PRP21,PRPF21,SAP114,SF3A120
General protein information	Preferred Names splicing factor 3a subunit 1 Names splicing factor 3A subunit 1 SAP 114 pre-mRNA processing 21 pre-mRNA splicing factor SF3a (120 kDa subunit) spliceosome-associated protein 114 splicing factor 3 subunit 1 splicing factor 3a, subunit 1, 120kD splicing factor 3a, subunit 1, 120kDa
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 22 Map Location: 22q12.2
Summary	This gene encodes a subunit of the splicing factor 3a protein complex. The splicing factor 3a heterotrimer is a component of the mature U2 small nuclear ribonucleoprotein particle (snRNP). U2 small nuclear ribonucleoproteins play a critical role in spliceosome assembly and pre-mRNA splicing. [provided by RefSeq, Aug 2014].
Disorder MIM:	605595

mRNA and Protein(s)

mRNA	Protein	Name
NM_005877.6	NP_005868.1	splicing factor 3A subunit 1
NM_005877.5	NP_005868.1	splicing factor 3A subunit 1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Sf3a1	NP_001100705.1
Schizosaccharomyces pombe (fission yeast)	sap114	NP_593239.1
Magnaporthe oryzae (rice blast fungus)	MGG_02946	XP_003720761.1
	SF3A1	XP_001109090.1
Canis lupus familiaris (dog)	SF3A1	XP_534733.2
Mus musculus (house mouse)	Sf3a1	NP_080451.4
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011328	XP_564160.3
Eremothecium gossypii	AGOS_AFR645W	NP_986193.2
Bos taurus (cattle)	SF3A1	NP_001074979.1
Danio rerio (zebrafish)	sf3a1	NP_956388.1
Neurospora crassa	NCU01913	XP_965544.1
Caenorhabditis elegans (roundworm)	prp-21	NP_493746.1
Xenopus tropicalis (tropical clawed frog)	LOC734097	XP_004910689.1
Homo sapiens (human)	SF3A1	NP_005868.1
Pan troglodytes (chimpanzee)	SF3A1	XP_515067.2
Gallus gallus (chicken)	SF3A1	NP_001026643.1
Drosophila melanogaster (fruit fly)	CG16941	NP_650583.1
Kluyveromyces lactis	KLLA0C03322g	XP_452346.1

Gene Ontology
Bibliography

Related articles in PubMed

Structure of the human activated spliceosome in three conformational states.
Zhang X, Yan C, Zhan X, Li L, Lei J, Shi Y
Cell research(2018 Jan)

A new role for FBP21 as regulator of Brr2 helicase activity.
Henning LM, Santos KF, Sticht J, Jehle S, Lee CT, Wittwer M, Urlaub H, Stelzl U, Wahl MC, Freund C
Nucleic acids research45(13)7922-7937(2017 Jul)

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

SF3A1 and pancreatic cancer: new evidence for the association of the spliceosome and cancer.
Tian J, Liu Y, Zhu B, Tian Y, Zhong R, Chen W, Lu X, Zou L, Shen N, Qian J, Li H, Miao X, Wang L
Oncotarget6(35)37750-7(2015 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Polymorphisms in SF3A1 gene is associated with pancreatic cancer.
Title: SF3A1 and pancreatic cancer: new evidence for the association of the spliceosome and cancer.

SF3A1 polymorphisms are not associated with Colorectal Cancer Risk.
Title: The Associations between RNA Splicing Complex Gene SF3A1 Polymorphisms and Colorectal Cancer Risk in a Chinese Population.

show that U1-SL4 interacts with the SF3A1 protein of the U2 snRNP
Title: Stem-loop 4 of U1 snRNA is essential for splicing and interacts with the U2 snRNP-specific SF3A1 protein during spliceosome assembly.

the SF3A mRNA splicing complex controls production of a negative regulator of TLR signaling that limits the extent of innate immune activation.
Title: Limiting of the innate immune response by SF3A-dependent control of MyD88 alternative mRNA splicing.

Clinical trial of gene-disease association and gene-environment interaction. (HuGE Navigator)
Title: Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score.

The following SF3A1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SF3A1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu26559	NM_005877.6 Latest version!	Homo sapiens splicing factor 3a subunit 1 (SF3A1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
View Old Accession Versions >>
OHu26559	NM_005877.5	Homo sapiens splicing factor 3a subunit 1 (SF3A1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SF3A1 ( NM_005877.6 ) cDNA ORF clone, Homo sapiens(human) -> NP_005868.1 Homo sapiens splicing factor 3a subunit 1 (SF3A1), mRNA.

Price & Availability↑

CloneID	OHu26559
Clone ID Related Accession (Same CDS sequence)	NM_005877.6 , NM_005877.5
Accession Version	NM_005877.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2382bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	splicing factor 3A subunit 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC007684.2, AC004997.2 and CK300534.1. On Nov 22, 2018 this sequence version replaced NM_005877.5. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.875500.1, SRR3476690.853979.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000215793.13/ ENSP00000215793.7 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCCGGCCG GACCCGTGCA GGCGGTGCCC CCGCCGCCGC CCGTGCCCAC GGAGCCCAAA 
CAGCCCACAG AAGAAGAAGC ATCTTCAAAG GAGGATTCTG CACCTTCTAA GCCAGTTGTG 
GGGATTATTT ACCCTCCTCC AGAGGTCAGA AATATTGTTG ACAAGACTGC CAGCTTTGTG 
GCCAGAAACG GGCCTGAATT TGAAGCTAGG ATCCGACAGA ACGAGATCAA CAACCCCAAG 
TTCAACTTTC TGAACCCCAA TGACCCTTAC CATGCCTACT ACCGCCACAA GGTCAGCGAG 
TTCAAGGAAG GGAAGGCTCA GGAGCCGTCC GCCGCCATCC CCAAGGTCAT GCAGCAGCAG 
CAGCAGACCA CCCAGCAGCA GCTGCCCCAG AAGGTCCAAG CCCAAGTAAT CCAAGAGACC 
ATCGTGCCCA AAGAGCCTCC TCCTGAGTTT GAGTTCATTG CTGATCCTCC CTCTATCTCA 
GCCTTCGACT TGGATGTGGT GAAGCTGACG GCTCAGTTTG TGGCCAGGAA TGGGCGCCAG 
TTTCTGACCC AGCTGATGCA GAAAGAGCAG CGCAACTACC AGTTTGACTT TCTCCGCCCA 
CAGCACAGCC TCTTCAACTA CTTCACGAAG CTAGTGGAAC AGTACACCAA GATCTTGATT 
CCACCCAAAG GTTTATTTTC AAAGCTCAAG AAAGAGGCTG AAAACCCCCG AGAAGTTTTG 
GATCAGGTGT GTTACCGAGT GGAATGGGCC AAATTCCAGG AACGTGAGAG GAAGAAGGAA 
GAAGAGGAGA AGGAGAAGGA GCGGGTGGCC TATGCTCAGA TCGACTGGCA TGATTTTGTG 
GTGGTGGAAA CAGTGGACTT CCAACCCAAT GAGCAAGGGA ACTTCCCTCC CCCCACCACG 
CCAGAGGAGC TGGGGGCCCG AATCCTCATT CAGGAGCGCT ATGAAAAGTT TGGGGAGAGT 
GAGGAAGTTG AGATGGAGGT CGAGTCTGAT GAGGAGGATG ACAAACAGGA GAAGGCGGAG 
GAGCCTCCTT CCCAGCTGGA CCAGGACACC CAAGTACAAG ATATGGATGA GGGTTCAGAT 
GATGAAGAAG AAGGGCAGAA AGTGCCCCCA CCCCCAGAGA CACCCATGCC TCCACCTCTG 
CCCCCAACTC CAGACCAAGT CATTGTCCGC AAGGATTATG ATCCCAAAGC CTCCAAGCCC 
TTGCCTCCAG CCCCTGCTCC AGATGAGTAT CTTGTGTCCC CCATTACTGG GGAGAAGATC 
CCCGCCAGCA AAATGCAGGA ACACATGCGC ATTGGACTTC TTGACCCTCG CTGGCTGGAG 
CAGCGGGATC GCTCCATCCG TGAGAAGCAG AGCGATGATG AGGTGTACGC ACCAGGTCTG 
GATATTGAGA GCAGCTTGAA GCAGTTGGCT GAGCGGCGTA CTGACATCTT CGGTGTAGAG 
GAAACAGCCA TTGGTAAGAA GATCGGTGAG GAGGAGATCC AGAAGCCAGA GGAAAAGGTG 
ACCTGGGATG GCCACTCAGG CAGCATGGCC CGGACCCAGC AGGCTGCCCA GGCCAACATC 
ACCCTCCAGG AGCAGATTGA GGCCATTCAC AAGGCCAAAG GCCTGGTGCC AGAGGATGAC 
ACTAAAGAGA AGATTGGCCC CAGCAAGCCC AATGAAATCC CTCAACAGCC ACCGCCACCA 
TCTTCAGCCA CCAACATCCC CAGCTCGGCT CCACCCATCA CTTCAGTGCC CCGACCACCC 
ACAATGCCAC CTCCAGTTCG TACTACAGTT GTCTCCGCAG TACCCGTCAT GCCCCGGCCC 
CCAATGGCAT CTGTGGTCCG GCTGCCCCCA GGCTCAGTGA TCGCCCCCAT GCCGCCCATC 
ATCCACGCGC CCAGAATCAA CGTGGTGCCC ATGCCTCCCT CGGCCCCTCC TATTATGGCC 
CCCCGCCCAC CCCCCATGAT TGTGCCAACA GCCTTTGTGC CTGCTCCACC TGTGGCACCT 
GTCCCAGCTC CAGCCCCAAT GCCCCCTGTG CATCCCCCAC CTCCCATGGA AGATGAGCCC 
ACCTCCAAAA AACTGAAGAC AGAGGACAGC CTCATGCCAG AGGAGGAGTT CCTGCGCAGA 
AACAAGGGTC CAGTGTCCAT CAAAGTCCAG GTGCCCAACA TGCAGGATAA GACGGAATGG 
AAACTGAATG GGCAGGTGCT GGTCTTCACC CTCCCACTCA CGGACCAGGT CTCTGTCATT 
AAGGTGAAGA TTCATGAAGC CACAGGCATG CCTGCAGGGA AACAGAAGCT ACAGTATGAG 
GGTATCTTCA TCAAAGATTC CAACTCACTG GCTTACTACA ACATGGCCAA TGGCGCAGTC 
ATCCACCTGG CCCTCAAGGA GAGAGGCGGG AGGAAGAAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005868.1
CDS	103..2484
Translation	MPAGPVQAVPPPPPVPTEPKQPTEEEASSKEDSAPSKPVVGIIYPPPEVRNIVDKTASFVARNGPEFEARIRQNEINNPKFNFLNPNDPYHAYYRHKVSEFKEGKAQEPSAAIPKVMQQQQQTTQQQLPQKVQAQVIQETIVPKEPPPEFEFIADPPSISAFDLDVVKLTAQFVARNGRQFLTQLMQKEQRNYQFDFLRPQHSLFNYFTKLVEQYTKILIPPKGLFSKLKKEAENPREVLDQVCYRVEWAKFQERERKKEEEEKEKERVAYAQIDWHDFVVVETVDFQPNEQGNFPPPTTPEELGARILIQERYEKFGESEEVEMEVESDEEDDKQEKAEEPPSQLDQDTQVQDMDEGSDDEEEGQKVPPPPETPMPPPLPPTPDQVIVRKDYDPKASKPLPPAPAPDEYLVSPITGEKIPASKMQEHMRIGLLDPRWLEQRDRSIREKQSDDEVYAPGLDIESSLKQLAERRTDIFGVEETAIGKKIGEEEIQKPEEKVTWDGHSGSMARTQQAAQANITLQEQIEAIHKAKGLVPEDDTKEKIGPSKPNEIPQQPPPPSSATNIPSSAPPITSVPRPPTMPPPVRTTVVSAVPVMPRPPMASVVRLPPGSVIAPMPPIIHAPRINVVPMPPSAPPIMAPRPPPMIVPTAFVPAPPVAPVPAPAPMPPVHPPPPMEDEPTSKKLKTEDSLMPEEEFLRRNKGPVSIKVQVPNMQDKTEWKLNGQVLVFTLPLTDQVSVIKVKIHEATGMPAGKQKLQYEGIFIKDSNSLAYYNMANGAVIHLALKERGGRKK

Target ORF information:

RefSeq Version	NM_005877.6
Organism	Homo sapiens(human)
Definition	Homo sapiens splicing factor 3a subunit 1 (SF3A1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005877.6

ORF Insert Sequence:

ATGCCGGCCG GACCCGTGCA GGCGGTGCCC CCGCCGCCGC CCGTGCCCAC GGAGCCCAAA 
CAGCCCACAG AAGAAGAAGC ATCTTCAAAG GAGGATTCTG CACCTTCTAA GCCAGTTGTG 
GGGATTATTT ACCCTCCTCC AGAGGTCAGA AATATTGTTG ACAAGACTGC CAGCTTTGTG 
GCCAGAAACG GGCCTGAATT TGAAGCTAGG ATCCGACAGA ACGAGATCAA CAACCCCAAG 
TTCAACTTTC TGAACCCCAA TGACCCTTAC CATGCCTACT ACCGCCACAA GGTCAGCGAG 
TTCAAGGAAG GGAAGGCTCA GGAGCCGTCC GCCGCCATCC CCAAGGTCAT GCAGCAGCAG 
CAGCAGACCA CCCAGCAGCA GCTGCCCCAG AAGGTCCAAG CCCAAGTAAT CCAAGAGACC 
ATCGTGCCCA AAGAGCCTCC TCCTGAGTTT GAGTTCATTG CTGATCCTCC CTCTATCTCA 
GCCTTCGACT TGGATGTGGT GAAGCTGACG GCTCAGTTTG TGGCCAGGAA TGGGCGCCAG 
TTTCTGACCC AGCTGATGCA GAAAGAGCAG CGCAACTACC AGTTTGACTT TCTCCGCCCA 
CAGCACAGCC TCTTCAACTA CTTCACGAAG CTAGTGGAAC AGTACACCAA GATCTTGATT 
CCACCCAAAG GTTTATTTTC AAAGCTCAAG AAAGAGGCTG AAAACCCCCG AGAAGTTTTG 
GATCAGGTGT GTTACCGAGT GGAATGGGCC AAATTCCAGG AACGTGAGAG GAAGAAGGAA 
GAAGAGGAGA AGGAGAAGGA GCGGGTGGCC TATGCTCAGA TCGACTGGCA TGATTTTGTG 
GTGGTGGAAA CAGTGGACTT CCAACCCAAT GAGCAAGGGA ACTTCCCTCC CCCCACCACG 
CCAGAGGAGC TGGGGGCCCG AATCCTCATT CAGGAGCGCT ATGAAAAGTT TGGGGAGAGT 
GAGGAAGTTG AGATGGAGGT CGAGTCTGAT GAGGAGGATG ACAAACAGGA GAAGGCGGAG 
GAGCCTCCTT CCCAGCTGGA CCAGGACACC CAAGTACAAG ATATGGATGA GGGTTCAGAT 
GATGAAGAAG AAGGGCAGAA AGTGCCCCCA CCCCCAGAGA CACCCATGCC TCCACCTCTG 
CCCCCAACTC CAGACCAAGT CATTGTCCGC AAGGATTATG ATCCCAAAGC CTCCAAGCCC 
TTGCCTCCAG CCCCTGCTCC AGATGAGTAT CTTGTGTCCC CCATTACTGG GGAGAAGATC 
CCCGCCAGCA AAATGCAGGA ACACATGCGC ATTGGACTTC TTGACCCTCG CTGGCTGGAG 
CAGCGGGATC GCTCCATCCG TGAGAAGCAG AGCGATGATG AGGTGTACGC ACCAGGTCTG 
GATATTGAGA GCAGCTTGAA GCAGTTGGCT GAGCGGCGTA CTGACATCTT CGGTGTAGAG 
GAAACAGCCA TTGGTAAGAA GATCGGTGAG GAGGAGATCC AGAAGCCAGA GGAAAAGGTG 
ACCTGGGATG GCCACTCAGG CAGCATGGCC CGGACCCAGC AGGCTGCCCA GGCCAACATC 
ACCCTCCAGG AGCAGATTGA GGCCATTCAC AAGGCCAAAG GCCTGGTGCC AGAGGATGAC 
ACTAAAGAGA AGATTGGCCC CAGCAAGCCC AATGAAATCC CTCAACAGCC ACCGCCACCA 
TCTTCAGCCA CCAACATCCC CAGCTCGGCT CCACCCATCA CTTCAGTGCC CCGACCACCC 
ACAATGCCAC CTCCAGTTCG TACTACAGTT GTCTCCGCAG TACCCGTCAT GCCCCGGCCC 
CCAATGGCAT CTGTGGTCCG GCTGCCCCCA GGCTCAGTGA TCGCCCCCAT GCCGCCCATC 
ATCCACGCGC CCAGAATCAA CGTGGTGCCC ATGCCTCCCT CGGCCCCTCC TATTATGGCC 
CCCCGCCCAC CCCCCATGAT TGTGCCAACA GCCTTTGTGC CTGCTCCACC TGTGGCACCT 
GTCCCAGCTC CAGCCCCAAT GCCCCCTGTG CATCCCCCAC CTCCCATGGA AGATGAGCCC 
ACCTCCAAAA AACTGAAGAC AGAGGACAGC CTCATGCCAG AGGAGGAGTT CCTGCGCAGA 
AACAAGGGTC CAGTGTCCAT CAAAGTCCAG GTGCCCAACA TGCAGGATAA GACGGAATGG 
AAACTGAATG GGCAGGTGCT GGTCTTCACC CTCCCACTCA CGGACCAGGT CTCTGTCATT 
AAGGTGAAGA TTCATGAAGC CACAGGCATG CCTGCAGGGA AACAGAAGCT ACAGTATGAG 
GGTATCTTCA TCAAAGATTC CAACTCACTG GCTTACTACA ACATGGCCAA TGGCGCAGTC 
ATCCACCTGG CCCTCAAGGA GAGAGGCGGG AGGAAGAAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SF3A1 ( NM_005877.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_005868.1 Homo sapiens splicing factor 3a subunit 1 (SF3A1), mRNA.

Price & Availability↑

CloneID	OHu26559
Clone ID Related Accession (Same CDS sequence)	NM_005877.6 , NM_005877.5
Accession Version	NM_005877.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2382bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-02
Organism	Homo sapiens(human)
Product	splicing factor 3A subunit 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB445415.1, BC007684.2, AC004997.2 and CK300534.1. On Aug 20, 2014 this sequence version replaced NM_005877.4. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC007684.2, X85237.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCGGCCG GACCCGTGCA GGCGGTGCCC CCGCCGCCGC CCGTGCCCAC GGAGCCCAAA 
CAGCCCACAG AAGAAGAAGC ATCTTCAAAG GAGGATTCTG CACCTTCTAA GCCAGTTGTG 
GGGATTATTT ACCCTCCTCC AGAGGTCAGA AATATTGTTG ACAAGACTGC CAGCTTTGTG 
GCCAGAAACG GGCCTGAATT TGAAGCTAGG ATCCGACAGA ACGAGATCAA CAACCCCAAG 
TTCAACTTTC TGAACCCCAA TGACCCTTAC CATGCCTACT ACCGCCACAA GGTCAGCGAG 
TTCAAGGAAG GGAAGGCTCA GGAGCCGTCC GCCGCCATCC CCAAGGTCAT GCAGCAGCAG 
CAGCAGACCA CCCAGCAGCA GCTGCCCCAG AAGGTCCAAG CCCAAGTAAT CCAAGAGACC 
ATCGTGCCCA AAGAGCCTCC TCCTGAGTTT GAGTTCATTG CTGATCCTCC CTCTATCTCA 
GCCTTCGACT TGGATGTGGT GAAGCTGACG GCTCAGTTTG TGGCCAGGAA TGGGCGCCAG 
TTTCTGACCC AGCTGATGCA GAAAGAGCAG CGCAACTACC AGTTTGACTT TCTCCGCCCA 
CAGCACAGCC TCTTCAACTA CTTCACGAAG CTAGTGGAAC AGTACACCAA GATCTTGATT 
CCACCCAAAG GTTTATTTTC AAAGCTCAAG AAAGAGGCTG AAAACCCCCG AGAAGTTTTG 
GATCAGGTGT GTTACCGAGT GGAATGGGCC AAATTCCAGG AACGTGAGAG GAAGAAGGAA 
GAAGAGGAGA AGGAGAAGGA GCGGGTGGCC TATGCTCAGA TCGACTGGCA TGATTTTGTG 
GTGGTGGAAA CAGTGGACTT CCAACCCAAT GAGCAAGGGA ACTTCCCTCC CCCCACCACG 
CCAGAGGAGC TGGGGGCCCG AATCCTCATT CAGGAGCGCT ATGAAAAGTT TGGGGAGAGT 
GAGGAAGTTG AGATGGAGGT CGAGTCTGAT GAGGAGGATG ACAAACAGGA GAAGGCGGAG 
GAGCCTCCTT CCCAGCTGGA CCAGGACACC CAAGTACAAG ATATGGATGA GGGTTCAGAT 
GATGAAGAAG AAGGGCAGAA AGTGCCCCCA CCCCCAGAGA CACCCATGCC TCCACCTCTG 
CCCCCAACTC CAGACCAAGT CATTGTCCGC AAGGATTATG ATCCCAAAGC CTCCAAGCCC 
TTGCCTCCAG CCCCTGCTCC AGATGAGTAT CTTGTGTCCC CCATTACTGG GGAGAAGATC 
CCCGCCAGCA AAATGCAGGA ACACATGCGC ATTGGACTTC TTGACCCTCG CTGGCTGGAG 
CAGCGGGATC GCTCCATCCG TGAGAAGCAG AGCGATGATG AGGTGTACGC ACCAGGTCTG 
GATATTGAGA GCAGCTTGAA GCAGTTGGCT GAGCGGCGTA CTGACATCTT CGGTGTAGAG 
GAAACAGCCA TTGGTAAGAA GATCGGTGAG GAGGAGATCC AGAAGCCAGA GGAAAAGGTG 
ACCTGGGATG GCCACTCAGG CAGCATGGCC CGGACCCAGC AGGCTGCCCA GGCCAACATC 
ACCCTCCAGG AGCAGATTGA GGCCATTCAC AAGGCCAAAG GCCTGGTGCC AGAGGATGAC 
ACTAAAGAGA AGATTGGCCC CAGCAAGCCC AATGAAATCC CTCAACAGCC ACCGCCACCA 
TCTTCAGCCA CCAACATCCC CAGCTCGGCT CCACCCATCA CTTCAGTGCC CCGACCACCC 
ACAATGCCAC CTCCAGTTCG TACTACAGTT GTCTCCGCAG TACCCGTCAT GCCCCGGCCC 
CCAATGGCAT CTGTGGTCCG GCTGCCCCCA GGCTCAGTGA TCGCCCCCAT GCCGCCCATC 
ATCCACGCGC CCAGAATCAA CGTGGTGCCC ATGCCTCCCT CGGCCCCTCC TATTATGGCC 
CCCCGCCCAC CCCCCATGAT TGTGCCAACA GCCTTTGTGC CTGCTCCACC TGTGGCACCT 
GTCCCAGCTC CAGCCCCAAT GCCCCCTGTG CATCCCCCAC CTCCCATGGA AGATGAGCCC 
ACCTCCAAAA AACTGAAGAC AGAGGACAGC CTCATGCCAG AGGAGGAGTT CCTGCGCAGA 
AACAAGGGTC CAGTGTCCAT CAAAGTCCAG GTGCCCAACA TGCAGGATAA GACGGAATGG 
AAACTGAATG GGCAGGTGCT GGTCTTCACC CTCCCACTCA CGGACCAGGT CTCTGTCATT 
AAGGTGAAGA TTCATGAAGC CACAGGCATG CCTGCAGGGA AACAGAAGCT ACAGTATGAG 
GGTATCTTCA TCAAAGATTC CAACTCACTG GCTTACTACA ACATGGCCAA TGGCGCAGTC 
ATCCACCTGG CCCTCAAGGA GAGAGGCGGG AGGAAGAAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005868.1
CDS	156..2537
Translation	MPAGPVQAVPPPPPVPTEPKQPTEEEASSKEDSAPSKPVVGIIYPPPEVRNIVDKTASFVARNGPEFEARIRQNEINNPKFNFLNPNDPYHAYYRHKVSEFKEGKAQEPSAAIPKVMQQQQQTTQQQLPQKVQAQVIQETIVPKEPPPEFEFIADPPSISAFDLDVVKLTAQFVARNGRQFLTQLMQKEQRNYQFDFLRPQHSLFNYFTKLVEQYTKILIPPKGLFSKLKKEAENPREVLDQVCYRVEWAKFQERERKKEEEEKEKERVAYAQIDWHDFVVVETVDFQPNEQGNFPPPTTPEELGARILIQERYEKFGESEEVEMEVESDEEDDKQEKAEEPPSQLDQDTQVQDMDEGSDDEEEGQKVPPPPETPMPPPLPPTPDQVIVRKDYDPKASKPLPPAPAPDEYLVSPITGEKIPASKMQEHMRIGLLDPRWLEQRDRSIREKQSDDEVYAPGLDIESSLKQLAERRTDIFGVEETAIGKKIGEEEIQKPEEKVTWDGHSGSMARTQQAAQANITLQEQIEAIHKAKGLVPEDDTKEKIGPSKPNEIPQQPPPPSSATNIPSSAPPITSVPRPPTMPPPVRTTVVSAVPVMPRPPMASVVRLPPGSVIAPMPPIIHAPRINVVPMPPSAPPIMAPRPPPMIVPTAFVPAPPVAPVPAPAPMPPVHPPPPMEDEPTSKKLKTEDSLMPEEEFLRRNKGPVSIKVQVPNMQDKTEWKLNGQVLVFTLPLTDQVSVIKVKIHEATGMPAGKQKLQYEGIFIKDSNSLAYYNMANGAVIHLALKERGGRKK

Target ORF information:

RefSeq Version	NM_005877.5
Organism	Homo sapiens(human)
Definition	Homo sapiens splicing factor 3a subunit 1 (SF3A1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005877.5

ORF Insert Sequence:

ATGCCGGCCG GACCCGTGCA GGCGGTGCCC CCGCCGCCGC CCGTGCCCAC GGAGCCCAAA 
CAGCCCACAG AAGAAGAAGC ATCTTCAAAG GAGGATTCTG CACCTTCTAA GCCAGTTGTG 
GGGATTATTT ACCCTCCTCC AGAGGTCAGA AATATTGTTG ACAAGACTGC CAGCTTTGTG 
GCCAGAAACG GGCCTGAATT TGAAGCTAGG ATCCGACAGA ACGAGATCAA CAACCCCAAG 
TTCAACTTTC TGAACCCCAA TGACCCTTAC CATGCCTACT ACCGCCACAA GGTCAGCGAG 
TTCAAGGAAG GGAAGGCTCA GGAGCCGTCC GCCGCCATCC CCAAGGTCAT GCAGCAGCAG 
CAGCAGACCA CCCAGCAGCA GCTGCCCCAG AAGGTCCAAG CCCAAGTAAT CCAAGAGACC 
ATCGTGCCCA AAGAGCCTCC TCCTGAGTTT GAGTTCATTG CTGATCCTCC CTCTATCTCA 
GCCTTCGACT TGGATGTGGT GAAGCTGACG GCTCAGTTTG TGGCCAGGAA TGGGCGCCAG 
TTTCTGACCC AGCTGATGCA GAAAGAGCAG CGCAACTACC AGTTTGACTT TCTCCGCCCA 
CAGCACAGCC TCTTCAACTA CTTCACGAAG CTAGTGGAAC AGTACACCAA GATCTTGATT 
CCACCCAAAG GTTTATTTTC AAAGCTCAAG AAAGAGGCTG AAAACCCCCG AGAAGTTTTG 
GATCAGGTGT GTTACCGAGT GGAATGGGCC AAATTCCAGG AACGTGAGAG GAAGAAGGAA 
GAAGAGGAGA AGGAGAAGGA GCGGGTGGCC TATGCTCAGA TCGACTGGCA TGATTTTGTG 
GTGGTGGAAA CAGTGGACTT CCAACCCAAT GAGCAAGGGA ACTTCCCTCC CCCCACCACG 
CCAGAGGAGC TGGGGGCCCG AATCCTCATT CAGGAGCGCT ATGAAAAGTT TGGGGAGAGT 
GAGGAAGTTG AGATGGAGGT CGAGTCTGAT GAGGAGGATG ACAAACAGGA GAAGGCGGAG 
GAGCCTCCTT CCCAGCTGGA CCAGGACACC CAAGTACAAG ATATGGATGA GGGTTCAGAT 
GATGAAGAAG AAGGGCAGAA AGTGCCCCCA CCCCCAGAGA CACCCATGCC TCCACCTCTG 
CCCCCAACTC CAGACCAAGT CATTGTCCGC AAGGATTATG ATCCCAAAGC CTCCAAGCCC 
TTGCCTCCAG CCCCTGCTCC AGATGAGTAT CTTGTGTCCC CCATTACTGG GGAGAAGATC 
CCCGCCAGCA AAATGCAGGA ACACATGCGC ATTGGACTTC TTGACCCTCG CTGGCTGGAG 
CAGCGGGATC GCTCCATCCG TGAGAAGCAG AGCGATGATG AGGTGTACGC ACCAGGTCTG 
GATATTGAGA GCAGCTTGAA GCAGTTGGCT GAGCGGCGTA CTGACATCTT CGGTGTAGAG 
GAAACAGCCA TTGGTAAGAA GATCGGTGAG GAGGAGATCC AGAAGCCAGA GGAAAAGGTG 
ACCTGGGATG GCCACTCAGG CAGCATGGCC CGGACCCAGC AGGCTGCCCA GGCCAACATC 
ACCCTCCAGG AGCAGATTGA GGCCATTCAC AAGGCCAAAG GCCTGGTGCC AGAGGATGAC 
ACTAAAGAGA AGATTGGCCC CAGCAAGCCC AATGAAATCC CTCAACAGCC ACCGCCACCA 
TCTTCAGCCA CCAACATCCC CAGCTCGGCT CCACCCATCA CTTCAGTGCC CCGACCACCC 
ACAATGCCAC CTCCAGTTCG TACTACAGTT GTCTCCGCAG TACCCGTCAT GCCCCGGCCC 
CCAATGGCAT CTGTGGTCCG GCTGCCCCCA GGCTCAGTGA TCGCCCCCAT GCCGCCCATC 
ATCCACGCGC CCAGAATCAA CGTGGTGCCC ATGCCTCCCT CGGCCCCTCC TATTATGGCC 
CCCCGCCCAC CCCCCATGAT TGTGCCAACA GCCTTTGTGC CTGCTCCACC TGTGGCACCT 
GTCCCAGCTC CAGCCCCAAT GCCCCCTGTG CATCCCCCAC CTCCCATGGA AGATGAGCCC 
ACCTCCAAAA AACTGAAGAC AGAGGACAGC CTCATGCCAG AGGAGGAGTT CCTGCGCAGA 
AACAAGGGTC CAGTGTCCAT CAAAGTCCAG GTGCCCAACA TGCAGGATAA GACGGAATGG 
AAACTGAATG GGCAGGTGCT GGTCTTCACC CTCCCACTCA CGGACCAGGT CTCTGTCATT 
AAGGTGAAGA TTCATGAAGC CACAGGCATG CCTGCAGGGA AACAGAAGCT ACAGTATGAG 
GGTATCTTCA TCAAAGATTC CAACTCACTG GCTTACTACA ACATGGCCAA TGGCGCAGTC 
ATCCACCTGG CCCTCAAGGA GAGAGGCGGG AGGAAGAAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Structure of the human activated spliceosome in three conformational states.
Cell research(2018 Jan)
Zhang X,Yan C,Zhan X,Li L,Lei J,Shi Y

A new role for FBP21 as regulator of Brr2 helicase activity.
Nucleic acids research45(13)7922-7937(2017 Jul)
Henning LM,Santos KF,Sticht J,Jehle S,Lee CT,Wittwer M,Urlaub H,Stelzl U,Wahl MC,Freund C

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

SF3A1 and pancreatic cancer: new evidence for the association of the spliceosome and cancer.
Oncotarget6(35)37750-7(2015 Nov)
Tian J,Liu Y,Zhu B,Tian Y,Zhong R,Chen W,Lu X,Zou L,Shen N,Qian J,Li H,Miao X,Wang L

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SF3A1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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