EFS cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	EFS
Entrez Gene ID	10278
Full Name	embryonal Fyn-associated substrate
Synonyms	CAS3,CASS3,EFS1,EFS2,HEFS,SIN
General protein information	Preferred Names embryonal Fyn-associated substrate Names embryonal Fyn-associated substrate Cas scaffolding protein family member 3 signal transduction protein (SH3 containing)
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 14 Map Location: 14q11.2
Summary	The longest protein isoform encoded by this gene contains an SH3 domain, which is known to be important in intracellular signal transduction. The protein encoded by a similiar gene in mice was shown to bind to SH3 domains of protein-tyrosine kinases. The function of this gene is unknown. Three alternatively spliced variants encoding different isoforms have been described for this gene. [provided by RefSeq, Mar 2013].
Disorder MIM:	609906

mRNA and Protein(s)

mRNA	Protein	Name
NM_005864.3	NP_005855.1	embryonal Fyn-associated substrate isoform 1
NM_032459.3	NP_115835.1	embryonal Fyn-associated substrate isoform 2
XM_005267256.1	XP_005267313.1	embryonal Fyn-associated substrate isoform X1
NM_032459.2	NP_115835.1	embryonal Fyn-associated substrate isoform 2
NM_005864.4	NP_005855.1	embryonal Fyn-associated substrate isoform 1
XM_024449457.1	XP_024305225.1	embryonal Fyn-associated substrate isoform X2
NM_001277174.1	NP_001264103.1	embryonal Fyn-associated substrate isoform 3
XM_005267256.2	XP_005267313.1	embryonal Fyn-associated substrate isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	EFS	NP_005855.1
	EFS	XP_001107716.1
Rattus norvegicus (Norway rat)	Efs	NP_001099503.1
Canis lupus familiaris (dog)	EFS	XP_537374.3
Pan troglodytes (chimpanzee)	EFS	XP_509854.2
Bos taurus (cattle)	EFS	NP_001092380.1
Mus musculus (house mouse)	Efs	NP_034242.2

Gene Ontology
Bibliography

Related articles in PubMed

Embryonal Fyn-associated substrate (EFS) and CASS4: The lesser-known CAS protein family members.
Deneka A, Korobeynikov V, Golemis EA
Gene570(1)25-35(2015 Oct)

Decreased expression of EFS is correlated with the advanced prostate cancer.
Sertkaya S, Hamid SM, Dilsiz N, Varisli L
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine36(2)799-805(2015 Feb)

EFS shows biallelic methylation in uveal melanoma with poor prognosis as well as tissue-specific methylation.
Neumann LC, Weinh?usel A, Thomas S, Horsthemke B, Lohmann DR, Zeschnigk M
BMC cancer11380(2011 Aug)

New genetic associations detected in a host response study to hepatitis B vaccine.
Davila S, Froeling FE, Tan A, Bonnard C, Boland GJ, Snippe H, Hibberd ML, Seielstad M
Genes and immunity11(3)232-8(2010 Apr)

Systematic identification of SH3 domain-mediated human protein-protein interactions by peptide array target screening.
Wu C, Ma MH, Brown KR, Geisler M, Li L, Tzeng E, Jia CY, Jurisica I, Li SS
Proteomics7(11)1775-85(2007 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

EFS and CASS4 protein function in the context of the larger CAS family group. [Review]
Title: Embryonal Fyn-associated substrate (EFS) and CASS4: The lesser-known CAS protein family members.

we suggest that EFS should be considered as a novel tumor suppressor gene in prostate cancer.
Title: Decreased expression of EFS is correlated with the advanced prostate cancer.

Analysis of 16 Uveal melanoma showed full methylation of the EFS CpG island in 8 (50%), no methylation in 5(31%)and partial methylation in 3 (19%)tumors. Kaplan-Meier analysis revealed higher risk of metastatic progression for tumors with EFS methylation.
Title: EFS shows biallelic methylation in uveal melanoma with poor prognosis as well as tissue-specific methylation.

Observational study of gene-disease association. (HuGE Navigator)
Title: New genetic associations detected in a host response study to hepatitis B vaccine.

The following EFS gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EFS cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu05019	NM_005864.4 Latest version!	Homo sapiens embryonal Fyn-associated substrate (EFS), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$234.50-$328.30 $469.00
OHu00494	NM_001277174.1 Latest version!	Homo sapiens embryonal Fyn-associated substrate (EFS), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu00584	NM_032459.3 Latest version!	Homo sapiens embryonal Fyn-associated substrate (EFS), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$265.30 $379.00
OHu47476	XM_005267256.2 Latest version!	Homo sapiens embryonal Fyn-associated substrate (EFS), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu103854	XM_024449457.1 Latest version!	Homo sapiens embryonal Fyn-associated substrate (EFS), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
View Old Accession Versions >>
OHu00584	NM_032459.2	Homo sapiens embryonal Fyn-associated substrate (EFS), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$265.30 $379.00
OHu05019	NM_005864.3	Homo sapiens embryonal Fyn-associated substrate (EFS), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$234.50-$328.30 $469.00
OHu47476	XM_005267256.1	Homo sapiens embryonal Fyn-associated substrate (EFS), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

EFS ( NM_005864.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_005855.1 Homo sapiens embryonal Fyn-associated substrate (EFS), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu05019
Clone ID Related Accession (Same CDS sequence)	NM_005864.3 , NM_005864.4
Accession Version	NM_005864.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1686bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-02
Organism	Homo sapiens(human)
Product	embryonal Fyn-associated substrate isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB001466.1 and AA470981.1. On Mar 15, 2013 this sequence version replaced NM_005864.2. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). The N-terminal end of this isoform contains the SH3 domain. ##Evidence-Data-START## Transcript exon combination :: AB001466.1, AK314221.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2149178 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCATTG CCACGTCGAC CCAGCTGGCC CGGGCACTGT ATGACAACAC CGCTGAGTCC 
CCCCAGGAGC TGTCCTTCCG CCGAGGGGAT GTCCTACGGG TCCTGCAGAG AGAGGGCGCT 
GGTGGACTGG ACGGCTGGTG CCTCTGCTCC CTACACGGCC AGCAGGGCAT TGTGCCCGCC 
AACAGGGTGA AGCTCTTGCC TGCTGGCCCA GCACCCAAGC CCAGCCTCTC TCCTGCGTCC 
CCAGCCCAGC CTGGCTCACC ATATCCAGCC CCAGATCACA GCAATGAGGA CCAGGAGGTG 
TATGTGGTGC CGCCCCCAGC TCGGCCCTGT CCAACCTCAG GACCTCCAGC TGGACCTTGC 
CCACCCTCTC CTGACCTCAT CTACAAAATC CCCAGAGCTA GTGGGACCCA GCTGGCTGCT 
CCCAGAGATG CCTTGGAGGT CTACGATGTG CCCCCCACCG CCCTCCGGGT GCCCTCCAGT 
GGCCCCTATG ACTGCCCTGC CTCCTTTTCC CACCCTCTGA CCCGGGTTGC CCCGCAGCCC 
CCTGGAGAGG ATGATGCTCC CTATGATGTG CCTCTGACCC CAAAGCCACC TGCAGAGCTG 
GAACCAGATC TGGAGTGGGA AGGAGGCCGG GAGCCGGGGC CCCCCATCTA TGCTGCCCCC 
TCCAACCTGA AACGAGCGTC AGCCTTACTC AATTTGTATG AAGCACCCGA GGAACTGCTG 
GCAGACGGGG AGGGCGGGGG CACTGATGAG GGGATCTACG ATGTGCCTCT GCTGGGGCCA 
GAGGCTCCCC CTTCTCCAGA GCCCCCTGGA GCCTTGGCCT CCCATGACCA GGACACCCTG 
GCCCAGCTTC TGGCCAGAAG CCCCCCACCC CCACACAGGC CCCGGCTCCC CTCAGCTGAG 
AGCCTGTCCC GCCGCCCTCT GCCTGCCCTG CCTGTCCCTG AGGCCCCCAG CCCCTCCCCA 
GTGCCCTCTC CTGCCCCAGG CCGGAAGGGC AGCATCCAGG ACCGGCCTCT GCCCCCACCC 
CCACCCCGCC TGCCTGGTTA TGGAGGCCCC AAGGTCGAGG GGGATCCAGA GGGCAGGGAG 
ATGGAGGATG ACCCAGCAGG ACACCACAAT GAGTACGAGG GCATTCCGAT GGCCGAGGAG 
TATGACTATG TCCACCTGAA GGGCATGGAC AAAGCTCAGG GATCTAGGCC CCCGGATCAG 
GCCTGCACAG GGGATCCTGA ACTGCCCGAG AGGGGGATGC CGGCGCCGCA GGAGGCCCTG 
TCCCCAGGGG AGCCACTGGT TGTGTCCACC GGAGATCTGC AGCTCCTGTA CTTCTATGCT 
GGGCAATGCC AGAGCCACTA CTCAGCCCTG CAGGCAGCCG TGGCAGCCCT GATGTCCAGT 
ACCCAGGCTA ATCAGCCCCC GCGCCTTTTC GTGCCCCACA GCAAGAGGGT GGTGGTGGCT 
GCTCATCGCC TGGTGTTTGT TGGGGACACC CTGGGCCGGC TGGCAGCCTC TGCCCCTCTG 
AGAGCACAGG TCAGGGCTGC AGGTACAGCA CTGGGCCAGG CATTGCGGGC CACTGTGCTG 
GCTGTCAAGG GAGCTGCCCT GGGCTACCCA TCCAGCCCTG CCATCCAAGA GATGGTGCAG 
TGTGTAACAG AACTGGCAGG GCAGGCCCTG CAATTCACTA CCCTGCTCAC TAGCCTGGCT 
CCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005855.1
CDS	609..2294
Translation	MAIATSTQLARALYDNTAESPQELSFRRGDVLRVLQREGAGGLDGWCLCSLHGQQGIVPANRVKLLPAGPAPKPSLSPASPAQPGSPYPAPDHSNEDQEVYVVPPPARPCPTSGPPAGPCPPSPDLIYKIPRASGTQLAAPRDALEVYDVPPTALRVPSSGPYDCPASFSHPLTRVAPQPPGEDDAPYDVPLTPKPPAELEPDLEWEGGREPGPPIYAAPSNLKRASALLNLYEAPEELLADGEGGGTDEGIYDVPLLGPEAPPSPEPPGALASHDQDTLAQLLARSPPPPHRPRLPSAESLSRRPLPALPVPEAPSPSPVPSPAPGRKGSIQDRPLPPPPPRLPGYGGPKVEGDPEGREMEDDPAGHHNEYEGIPMAEEYDYVHLKGMDKAQGSRPPDQACTGDPELPERGMPAPQEALSPGEPLVVSTGDLQLLYFYAGQCQSHYSALQAAVAALMSSTQANQPPRLFVPHSKRVVVAAHRLVFVGDTLGRLAASAPLRAQVRAAGTALGQALRATVLAVKGAALGYPSSPAIQEMVQCVTELAGQALQFTTLLTSLAP

Target ORF information:

RefSeq Version	NM_005864.3
Organism	Homo sapiens(human)
Definition	Homo sapiens embryonal Fyn-associated substrate (EFS), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005864.3

ORF Insert Sequence:

ATGGCCATTG CCACGTCGAC CCAGCTGGCC CGGGCACTGT ATGACAACAC CGCTGAGTCC 
CCCCAGGAGC TGTCCTTCCG CCGAGGGGAT GTCCTACGGG TCCTGCAGAG AGAGGGCGCT 
GGTGGACTGG ACGGCTGGTG CCTCTGCTCC CTACACGGCC AGCAGGGCAT TGTGCCCGCC 
AACAGGGTGA AGCTCTTGCC TGCTGGCCCA GCACCCAAGC CCAGCCTCTC TCCTGCGTCC 
CCAGCCCAGC CTGGCTCACC ATATCCAGCC CCAGATCACA GCAATGAGGA CCAGGAGGTG 
TATGTGGTGC CGCCCCCAGC TCGGCCCTGT CCAACCTCAG GACCTCCAGC TGGACCTTGC 
CCACCCTCTC CTGACCTCAT CTACAAAATC CCCAGAGCTA GTGGGACCCA GCTGGCTGCT 
CCCAGAGATG CCTTGGAGGT CTACGATGTG CCCCCCACCG CCCTCCGGGT GCCCTCCAGT 
GGCCCCTATG ACTGCCCTGC CTCCTTTTCC CACCCTCTGA CCCGGGTTGC CCCGCAGCCC 
CCTGGAGAGG ATGATGCTCC CTATGATGTG CCTCTGACCC CAAAGCCACC TGCAGAGCTG 
GAACCAGATC TGGAGTGGGA AGGAGGCCGG GAGCCGGGGC CCCCCATCTA TGCTGCCCCC 
TCCAACCTGA AACGAGCGTC AGCCTTACTC AATTTGTATG AAGCACCCGA GGAACTGCTG 
GCAGACGGGG AGGGCGGGGG CACTGATGAG GGGATCTACG ATGTGCCTCT GCTGGGGCCA 
GAGGCTCCCC CTTCTCCAGA GCCCCCTGGA GCCTTGGCCT CCCATGACCA GGACACCCTG 
GCCCAGCTTC TGGCCAGAAG CCCCCCACCC CCACACAGGC CCCGGCTCCC CTCAGCTGAG 
AGCCTGTCCC GCCGCCCTCT GCCTGCCCTG CCTGTCCCTG AGGCCCCCAG CCCCTCCCCA 
GTGCCCTCTC CTGCCCCAGG CCGGAAGGGC AGCATCCAGG ACCGGCCTCT GCCCCCACCC 
CCACCCCGCC TGCCTGGTTA TGGAGGCCCC AAGGTCGAGG GGGATCCAGA GGGCAGGGAG 
ATGGAGGATG ACCCAGCAGG ACACCACAAT GAGTACGAGG GCATTCCGAT GGCCGAGGAG 
TATGACTATG TCCACCTGAA GGGCATGGAC AAAGCTCAGG GATCTAGGCC CCCGGATCAG 
GCCTGCACAG GGGATCCTGA ACTGCCCGAG AGGGGGATGC CGGCGCCGCA GGAGGCCCTG 
TCCCCAGGGG AGCCACTGGT TGTGTCCACC GGAGATCTGC AGCTCCTGTA CTTCTATGCT 
GGGCAATGCC AGAGCCACTA CTCAGCCCTG CAGGCAGCCG TGGCAGCCCT GATGTCCAGT 
ACCCAGGCTA ATCAGCCCCC GCGCCTTTTC GTGCCCCACA GCAAGAGGGT GGTGGTGGCT 
GCTCATCGCC TGGTGTTTGT TGGGGACACC CTGGGCCGGC TGGCAGCCTC TGCCCCTCTG 
AGAGCACAGG TCAGGGCTGC AGGTACAGCA CTGGGCCAGG CATTGCGGGC CACTGTGCTG 
GCTGTCAAGG GAGCTGCCCT GGGCTACCCA TCCAGCCCTG CCATCCAAGA GATGGTGCAG 
TGTGTAACAG AACTGGCAGG GCAGGCCCTG CAATTCACTA CCCTGCTCAC TAGCCTGGCT 
CCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EFS ( NM_005864.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_005855.1 Homo sapiens embryonal Fyn-associated substrate (EFS), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu05019
Clone ID Related Accession (Same CDS sequence)	NM_005864.3 , NM_005864.4
Accession Version	NM_005864.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1686bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-01
Organism	Homo sapiens(human)
Product	embryonal Fyn-associated substrate isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB001466.1. On Nov 23, 2018 this sequence version replaced NM_005864.3. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). The N-terminal end of this isoform contains the SH3 domain. ##Evidence-Data-START## Transcript exon combination :: AB001466.1, AK314221.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2149178 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000216733.8/ ENSP00000216733.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCCATTG CCACGTCGAC CCAGCTGGCC CGGGCACTGT ATGACAACAC CGCTGAGTCC 
CCCCAGGAGC TGTCCTTCCG CCGAGGGGAT GTCCTACGGG TCCTGCAGAG AGAGGGCGCT 
GGTGGACTGG ACGGCTGGTG CCTCTGCTCC CTACACGGCC AGCAGGGCAT TGTGCCCGCC 
AACAGGGTGA AGCTCTTGCC TGCTGGCCCA GCACCCAAGC CCAGCCTCTC TCCTGCGTCC 
CCAGCCCAGC CTGGCTCACC ATATCCAGCC CCAGATCACA GCAATGAGGA CCAGGAGGTG 
TATGTGGTGC CGCCCCCAGC TCGGCCCTGT CCAACCTCAG GACCTCCAGC TGGACCTTGC 
CCACCCTCTC CTGACCTCAT CTACAAAATC CCCAGAGCTA GTGGGACCCA GCTGGCTGCT 
CCCAGAGATG CCTTGGAGGT CTACGATGTG CCCCCCACCG CCCTCCGGGT GCCCTCCAGT 
GGCCCCTATG ACTGCCCTGC CTCCTTTTCC CACCCTCTGA CCCGGGTTGC CCCGCAGCCC 
CCTGGAGAGG ATGATGCTCC CTATGATGTG CCTCTGACCC CAAAGCCACC TGCAGAGCTG 
GAACCAGATC TGGAGTGGGA AGGAGGCCGG GAGCCGGGGC CCCCCATCTA TGCTGCCCCC 
TCCAACCTGA AACGAGCGTC AGCCTTACTC AATTTGTATG AAGCACCCGA GGAACTGCTG 
GCAGACGGGG AGGGCGGGGG CACTGATGAG GGGATCTACG ATGTGCCTCT GCTGGGGCCA 
GAGGCTCCCC CTTCTCCAGA GCCCCCTGGA GCCTTGGCCT CCCATGACCA GGACACCCTG 
GCCCAGCTTC TGGCCAGAAG CCCCCCACCC CCACACAGGC CCCGGCTCCC CTCAGCTGAG 
AGCCTGTCCC GCCGCCCTCT GCCTGCCCTG CCTGTCCCTG AGGCCCCCAG CCCCTCCCCA 
GTGCCCTCTC CTGCCCCAGG CCGGAAGGGC AGCATCCAGG ACCGGCCTCT GCCCCCACCC 
CCACCCCGCC TGCCTGGTTA TGGAGGCCCC AAGGTCGAGG GGGATCCAGA GGGCAGGGAG 
ATGGAGGATG ACCCAGCAGG ACACCACAAT GAGTACGAGG GCATTCCGAT GGCCGAGGAG 
TATGACTATG TCCACCTGAA GGGCATGGAC AAAGCTCAGG GATCTAGGCC CCCGGATCAG 
GCCTGCACAG GGGATCCTGA ACTGCCCGAG AGGGGGATGC CGGCGCCGCA GGAGGCCCTG 
TCCCCAGGGG AGCCACTGGT TGTGTCCACC GGAGATCTGC AGCTCCTGTA CTTCTATGCT 
GGGCAATGCC AGAGCCACTA CTCAGCCCTG CAGGCAGCCG TGGCAGCCCT GATGTCCAGT 
ACCCAGGCTA ATCAGCCCCC GCGCCTTTTC GTGCCCCACA GCAAGAGGGT GGTGGTGGCT 
GCTCATCGCC TGGTGTTTGT TGGGGACACC CTGGGCCGGC TGGCAGCCTC TGCCCCTCTG 
AGAGCACAGG TCAGGGCTGC AGGTACAGCA CTGGGCCAGG CATTGCGGGC CACTGTGCTG 
GCTGTCAAGG GAGCTGCCCT GGGCTACCCA TCCAGCCCTG CCATCCAAGA GATGGTGCAG 
TGTGTAACAG AACTGGCAGG GCAGGCCCTG CAATTCACTA CCCTGCTCAC TAGCCTGGCT 
CCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005855.1
CDS	148..1833
Translation	MAIATSTQLARALYDNTAESPQELSFRRGDVLRVLQREGAGGLDGWCLCSLHGQQGIVPANRVKLLPAGPAPKPSLSPASPAQPGSPYPAPDHSNEDQEVYVVPPPARPCPTSGPPAGPCPPSPDLIYKIPRASGTQLAAPRDALEVYDVPPTALRVPSSGPYDCPASFSHPLTRVAPQPPGEDDAPYDVPLTPKPPAELEPDLEWEGGREPGPPIYAAPSNLKRASALLNLYEAPEELLADGEGGGTDEGIYDVPLLGPEAPPSPEPPGALASHDQDTLAQLLARSPPPPHRPRLPSAESLSRRPLPALPVPEAPSPSPVPSPAPGRKGSIQDRPLPPPPPRLPGYGGPKVEGDPEGREMEDDPAGHHNEYEGIPMAEEYDYVHLKGMDKAQGSRPPDQACTGDPELPERGMPAPQEALSPGEPLVVSTGDLQLLYFYAGQCQSHYSALQAAVAALMSSTQANQPPRLFVPHSKRVVVAAHRLVFVGDTLGRLAASAPLRAQVRAAGTALGQALRATVLAVKGAALGYPSSPAIQEMVQCVTELAGQALQFTTLLTSLAP

Target ORF information:

RefSeq Version	NM_005864.4
Organism	Homo sapiens(human)
Definition	Homo sapiens embryonal Fyn-associated substrate (EFS), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005864.4

ORF Insert Sequence:

ATGGCCATTG CCACGTCGAC CCAGCTGGCC CGGGCACTGT ATGACAACAC CGCTGAGTCC 
CCCCAGGAGC TGTCCTTCCG CCGAGGGGAT GTCCTACGGG TCCTGCAGAG AGAGGGCGCT 
GGTGGACTGG ACGGCTGGTG CCTCTGCTCC CTACACGGCC AGCAGGGCAT TGTGCCCGCC 
AACAGGGTGA AGCTCTTGCC TGCTGGCCCA GCACCCAAGC CCAGCCTCTC TCCTGCGTCC 
CCAGCCCAGC CTGGCTCACC ATATCCAGCC CCAGATCACA GCAATGAGGA CCAGGAGGTG 
TATGTGGTGC CGCCCCCAGC TCGGCCCTGT CCAACCTCAG GACCTCCAGC TGGACCTTGC 
CCACCCTCTC CTGACCTCAT CTACAAAATC CCCAGAGCTA GTGGGACCCA GCTGGCTGCT 
CCCAGAGATG CCTTGGAGGT CTACGATGTG CCCCCCACCG CCCTCCGGGT GCCCTCCAGT 
GGCCCCTATG ACTGCCCTGC CTCCTTTTCC CACCCTCTGA CCCGGGTTGC CCCGCAGCCC 
CCTGGAGAGG ATGATGCTCC CTATGATGTG CCTCTGACCC CAAAGCCACC TGCAGAGCTG 
GAACCAGATC TGGAGTGGGA AGGAGGCCGG GAGCCGGGGC CCCCCATCTA TGCTGCCCCC 
TCCAACCTGA AACGAGCGTC AGCCTTACTC AATTTGTATG AAGCACCCGA GGAACTGCTG 
GCAGACGGGG AGGGCGGGGG CACTGATGAG GGGATCTACG ATGTGCCTCT GCTGGGGCCA 
GAGGCTCCCC CTTCTCCAGA GCCCCCTGGA GCCTTGGCCT CCCATGACCA GGACACCCTG 
GCCCAGCTTC TGGCCAGAAG CCCCCCACCC CCACACAGGC CCCGGCTCCC CTCAGCTGAG 
AGCCTGTCCC GCCGCCCTCT GCCTGCCCTG CCTGTCCCTG AGGCCCCCAG CCCCTCCCCA 
GTGCCCTCTC CTGCCCCAGG CCGGAAGGGC AGCATCCAGG ACCGGCCTCT GCCCCCACCC 
CCACCCCGCC TGCCTGGTTA TGGAGGCCCC AAGGTCGAGG GGGATCCAGA GGGCAGGGAG 
ATGGAGGATG ACCCAGCAGG ACACCACAAT GAGTACGAGG GCATTCCGAT GGCCGAGGAG 
TATGACTATG TCCACCTGAA GGGCATGGAC AAAGCTCAGG GATCTAGGCC CCCGGATCAG 
GCCTGCACAG GGGATCCTGA ACTGCCCGAG AGGGGGATGC CGGCGCCGCA GGAGGCCCTG 
TCCCCAGGGG AGCCACTGGT TGTGTCCACC GGAGATCTGC AGCTCCTGTA CTTCTATGCT 
GGGCAATGCC AGAGCCACTA CTCAGCCCTG CAGGCAGCCG TGGCAGCCCT GATGTCCAGT 
ACCCAGGCTA ATCAGCCCCC GCGCCTTTTC GTGCCCCACA GCAAGAGGGT GGTGGTGGCT 
GCTCATCGCC TGGTGTTTGT TGGGGACACC CTGGGCCGGC TGGCAGCCTC TGCCCCTCTG 
AGAGCACAGG TCAGGGCTGC AGGTACAGCA CTGGGCCAGG CATTGCGGGC CACTGTGCTG 
GCTGTCAAGG GAGCTGCCCT GGGCTACCCA TCCAGCCCTG CCATCCAAGA GATGGTGCAG 
TGTGTAACAG AACTGGCAGG GCAGGCCCTG CAATTCACTA CCCTGCTCAC TAGCCTGGCT 
CCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EFS ( NM_001277174.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001264103.1 Homo sapiens embryonal Fyn-associated substrate (EFS), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu00494
Clone ID Related Accession (Same CDS sequence)	NM_001277174.1
Accession Version	NM_001277174.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1179bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-24
Organism	Homo sapiens(human)
Product	embryonal Fyn-associated substrate isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB001466.1, AK295933.1 and AA470981.1. Transcript Variant: This variant (3) lacks an alternate in-frame exon and an alternate in-frame segment compared to variant 1. The resulting isoform (3) has the same N- and C-termini but is shorter compared to isoform 1. This isoform lacks the N-terminal SH3 domain found in isoform 1. ##Evidence-Data-START## Transcript exon combination :: AK295933.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03465403 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCATTG CCACGTCGGT GTATGTGGTG CCGCCCCCAG CTCGGCCCTG TCCAACCTCA 
GGACCTCCAG CTGGACCTTG CCCACCCTCT CCTGACCTCA TCTACAAAAT CCCCAGAGCT 
AGTGGGACCC AGCTGGCTGC TCCCAGAGAT GCCTTGGAGG TCTACGATGT GCCCCCCACC 
GCCCTCCGGG TGCCCTCCAG TGGCCCCTAT GACTGCCCTG CCTCCTTTTC CCACCCTCTG 
ACCCGGGTTG CCCCGCAGCC CCCTGGAGAG GATGATGCTC CCCCTTCTCC AGAGCCCCCT 
GGAGCCTTGG CCTCCCATGA CCAGGACACC CTGGCCCAGC TTCTGGCCAG AAGCCCCCCA 
CCCCCACACA GGCCCCGGCT CCCCTCAGCT GAGAGCCTGT CCCGCCGCCC TCTGCCTGCC 
CTGCCTGTCC CTGAGGCCCC CAGCCCCTCC CCAGTGCCCT CTCCTGCCCC AGGCCGGAAG 
GGCAGCATCC AGGACCGGCC TCTGCCCCCA CCCCCACCCC GCCTGCCTGG TTATGGAGGC 
CCCAAGGTCG AGGGGGATCC AGAGGGCAGG GAGATGGAGG ATGACCCAGC AGGACACCAC 
AATGAGTACG AGGGCATTCC GATGGCCGAG GAGTATGACT ATGTCCACCT GAAGGGCATG 
GACAAAGCTC AGGGATCTAG GCCCCCGGAT CAGGCCTGCA CAGGGGATCC TGAACTGCCC 
GAGAGGGGGA TGCCGGCGCC GCAGGAGGCC CTGTCCCCAG GGGAGCCACT GGTTGTGTCC 
ACCGGAGATC TGCAGCTCCT GTACTTCTAT GCTGGGCAAT GCCAGAGCCA CTACTCAGCC 
CTGCAGGCAG CCGTGGCAGC CCTGATGTCC AGTACCCAGG CTAATCAGCC CCCGCGCCTT 
TTCGTGCCCC ACAGCAAGAG GGTGGTGGTG GCTGCTCATC GCCTGGTGTT TGTTGGGGAC 
ACCCTGGGCC GGCTGGCAGC CTCTGCCCCT CTGAGAGCAC AGGTCAGGGC TGCAGGTACA 
GCACTGGGCC AGGCATTGCG GGCCACTGTG CTGGCTGTCA AGGGAGCTGC CCTGGGCTAC 
CCATCCAGCC CTGCCATCCA AGAGATGGTG CAGTGTGTAA CAGAACTGGC AGGGCAGGCC 
CTGCAATTCA CTACCCTGCT CACTAGCCTG GCTCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001264103.1
CDS	609..1787
Translation	MAIATSVYVVPPPARPCPTSGPPAGPCPPSPDLIYKIPRASGTQLAAPRDALEVYDVPPTALRVPSSGPYDCPASFSHPLTRVAPQPPGEDDAPPSPEPPGALASHDQDTLAQLLARSPPPPHRPRLPSAESLSRRPLPALPVPEAPSPSPVPSPAPGRKGSIQDRPLPPPPPRLPGYGGPKVEGDPEGREMEDDPAGHHNEYEGIPMAEEYDYVHLKGMDKAQGSRPPDQACTGDPELPERGMPAPQEALSPGEPLVVSTGDLQLLYFYAGQCQSHYSALQAAVAALMSSTQANQPPRLFVPHSKRVVVAAHRLVFVGDTLGRLAASAPLRAQVRAAGTALGQALRATVLAVKGAALGYPSSPAIQEMVQCVTELAGQALQFTTLLTSLAP

Target ORF information:

RefSeq Version	NM_001277174.1
Organism	Homo sapiens(human)
Definition	Homo sapiens embryonal Fyn-associated substrate (EFS), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001277174.1

ORF Insert Sequence:

ATGGCCATTG CCACGTCGGT GTATGTGGTG CCGCCCCCAG CTCGGCCCTG TCCAACCTCA 
GGACCTCCAG CTGGACCTTG CCCACCCTCT CCTGACCTCA TCTACAAAAT CCCCAGAGCT 
AGTGGGACCC AGCTGGCTGC TCCCAGAGAT GCCTTGGAGG TCTACGATGT GCCCCCCACC 
GCCCTCCGGG TGCCCTCCAG TGGCCCCTAT GACTGCCCTG CCTCCTTTTC CCACCCTCTG 
ACCCGGGTTG CCCCGCAGCC CCCTGGAGAG GATGATGCTC CCCCTTCTCC AGAGCCCCCT 
GGAGCCTTGG CCTCCCATGA CCAGGACACC CTGGCCCAGC TTCTGGCCAG AAGCCCCCCA 
CCCCCACACA GGCCCCGGCT CCCCTCAGCT GAGAGCCTGT CCCGCCGCCC TCTGCCTGCC 
CTGCCTGTCC CTGAGGCCCC CAGCCCCTCC CCAGTGCCCT CTCCTGCCCC AGGCCGGAAG 
GGCAGCATCC AGGACCGGCC TCTGCCCCCA CCCCCACCCC GCCTGCCTGG TTATGGAGGC 
CCCAAGGTCG AGGGGGATCC AGAGGGCAGG GAGATGGAGG ATGACCCAGC AGGACACCAC 
AATGAGTACG AGGGCATTCC GATGGCCGAG GAGTATGACT ATGTCCACCT GAAGGGCATG 
GACAAAGCTC AGGGATCTAG GCCCCCGGAT CAGGCCTGCA CAGGGGATCC TGAACTGCCC 
GAGAGGGGGA TGCCGGCGCC GCAGGAGGCC CTGTCCCCAG GGGAGCCACT GGTTGTGTCC 
ACCGGAGATC TGCAGCTCCT GTACTTCTAT GCTGGGCAAT GCCAGAGCCA CTACTCAGCC 
CTGCAGGCAG CCGTGGCAGC CCTGATGTCC AGTACCCAGG CTAATCAGCC CCCGCGCCTT 
TTCGTGCCCC ACAGCAAGAG GGTGGTGGTG GCTGCTCATC GCCTGGTGTT TGTTGGGGAC 
ACCCTGGGCC GGCTGGCAGC CTCTGCCCCT CTGAGAGCAC AGGTCAGGGC TGCAGGTACA 
GCACTGGGCC AGGCATTGCG GGCCACTGTG CTGGCTGTCA AGGGAGCTGC CCTGGGCTAC 
CCATCCAGCC CTGCCATCCA AGAGATGGTG CAGTGTGTAA CAGAACTGGC AGGGCAGGCC 
CTGCAATTCA CTACCCTGCT CACTAGCCTG GCTCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EFS ( NM_032459.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_115835.1 Homo sapiens embryonal Fyn-associated substrate (EFS), transcript variant 2, mRNA.

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CloneID	OHu00584
Clone ID Related Accession (Same CDS sequence)	NM_032459.3 , NM_032459.2
Accession Version	NM_032459.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1407bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-30
Organism	Homo sapiens(human)
Product	embryonal Fyn-associated substrate isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB001467.1 and AB001466.1. On May 31, 2019 this sequence version replaced NM_032459.2. Transcript Variant: This variant (2) lacks an alternate in-frame coding exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. This isoform lacks the N-terminal SH3 domain found in isoform 1. ##Evidence-Data-START## Transcript exon combination :: BC046403.1, AB001467.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCATTG CCACGTCGGT GTATGTGGTG CCGCCCCCAG CTCGGCCCTG TCCAACCTCA 
GGACCTCCAG CTGGACCTTG CCCACCCTCT CCTGACCTCA TCTACAAAAT CCCCAGAGCT 
AGTGGGACCC AGCTGGCTGC TCCCAGAGAT GCCTTGGAGG TCTACGATGT GCCCCCCACC 
GCCCTCCGGG TGCCCTCCAG TGGCCCCTAT GACTGCCCTG CCTCCTTTTC CCACCCTCTG 
ACCCGGGTTG CCCCGCAGCC CCCTGGAGAG GATGATGCTC CCTATGATGT GCCTCTGACC 
CCAAAGCCAC CTGCAGAGCT GGAACCAGAT CTGGAGTGGG AAGGAGGCCG GGAGCCGGGG 
CCCCCCATCT ATGCTGCCCC CTCCAACCTG AAACGAGCGT CAGCCTTACT CAATTTGTAT 
GAAGCACCCG AGGAACTGCT GGCAGACGGG GAGGGCGGGG GCACTGATGA GGGGATCTAC 
GATGTGCCTC TGCTGGGGCC AGAGGCTCCC CCTTCTCCAG AGCCCCCTGG AGCCTTGGCC 
TCCCATGACC AGGACACCCT GGCCCAGCTT CTGGCCAGAA GCCCCCCACC CCCACACAGG 
CCCCGGCTCC CCTCAGCTGA GAGCCTGTCC CGCCGCCCTC TGCCTGCCCT GCCTGTCCCT 
GAGGCCCCCA GCCCCTCCCC AGTGCCCTCT CCTGCCCCAG GCCGGAAGGG CAGCATCCAG 
GACCGGCCTC TGCCCCCACC CCCACCCCGC CTGCCTGGTT ATGGAGGCCC CAAGGTCGAG 
GGGGATCCAG AGGGCAGGGA GATGGAGGAT GACCCAGCAG GACACCACAA TGAGTACGAG 
GGCATTCCGA TGGCCGAGGA GTATGACTAT GTCCACCTGA AGGGCATGGA CAAAGCTCAG 
GGATCTAGGC CCCCGGATCA GGCCTGCACA GGGGATCCTG AACTGCCCGA GAGGGGGATG 
CCGGCGCCGC AGGAGGCCCT GTCCCCAGGG GAGCCACTGG TTGTGTCCAC CGGAGATCTG 
CAGCTCCTGT ACTTCTATGC TGGGCAATGC CAGAGCCACT ACTCAGCCCT GCAGGCAGCC 
GTGGCAGCCC TGATGTCCAG TACCCAGGCT AATCAGCCCC CGCGCCTTTT CGTGCCCCAC 
AGCAAGAGGG TGGTGGTGGC TGCTCATCGC CTGGTGTTTG TTGGGGACAC CCTGGGCCGG 
CTGGCAGCCT CTGCCCCTCT GAGAGCACAG GTCAGGGCTG CAGGTACAGC ACTGGGCCAG 
GCATTGCGGG CCACTGTGCT GGCTGTCAAG GGAGCTGCCC TGGGCTACCC ATCCAGCCCT 
GCCATCCAAG AGATGGTGCA GTGTGTAACA GAACTGGCAG GGCAGGCCCT GCAATTCACT 
ACCCTGCTCA CTAGCCTGGC TCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_115835.1
CDS	148..1554
Translation	MAIATSVYVVPPPARPCPTSGPPAGPCPPSPDLIYKIPRASGTQLAAPRDALEVYDVPPTALRVPSSGPYDCPASFSHPLTRVAPQPPGEDDAPYDVPLTPKPPAELEPDLEWEGGREPGPPIYAAPSNLKRASALLNLYEAPEELLADGEGGGTDEGIYDVPLLGPEAPPSPEPPGALASHDQDTLAQLLARSPPPPHRPRLPSAESLSRRPLPALPVPEAPSPSPVPSPAPGRKGSIQDRPLPPPPPRLPGYGGPKVEGDPEGREMEDDPAGHHNEYEGIPMAEEYDYVHLKGMDKAQGSRPPDQACTGDPELPERGMPAPQEALSPGEPLVVSTGDLQLLYFYAGQCQSHYSALQAAVAALMSSTQANQPPRLFVPHSKRVVVAAHRLVFVGDTLGRLAASAPLRAQVRAAGTALGQALRATVLAVKGAALGYPSSPAIQEMVQCVTELAGQALQFTTLLTSLAP

Target ORF information:

RefSeq Version	NM_032459.3
Organism	Homo sapiens(human)
Definition	Homo sapiens embryonal Fyn-associated substrate (EFS), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_032459.3

ORF Insert Sequence:

ATGGCCATTG CCACGTCGGT GTATGTGGTG CCGCCCCCAG CTCGGCCCTG TCCAACCTCA 
GGACCTCCAG CTGGACCTTG CCCACCCTCT CCTGACCTCA TCTACAAAAT CCCCAGAGCT 
AGTGGGACCC AGCTGGCTGC TCCCAGAGAT GCCTTGGAGG TCTACGATGT GCCCCCCACC 
GCCCTCCGGG TGCCCTCCAG TGGCCCCTAT GACTGCCCTG CCTCCTTTTC CCACCCTCTG 
ACCCGGGTTG CCCCGCAGCC CCCTGGAGAG GATGATGCTC CCTATGATGT GCCTCTGACC 
CCAAAGCCAC CTGCAGAGCT GGAACCAGAT CTGGAGTGGG AAGGAGGCCG GGAGCCGGGG 
CCCCCCATCT ATGCTGCCCC CTCCAACCTG AAACGAGCGT CAGCCTTACT CAATTTGTAT 
GAAGCACCCG AGGAACTGCT GGCAGACGGG GAGGGCGGGG GCACTGATGA GGGGATCTAC 
GATGTGCCTC TGCTGGGGCC AGAGGCTCCC CCTTCTCCAG AGCCCCCTGG AGCCTTGGCC 
TCCCATGACC AGGACACCCT GGCCCAGCTT CTGGCCAGAA GCCCCCCACC CCCACACAGG 
CCCCGGCTCC CCTCAGCTGA GAGCCTGTCC CGCCGCCCTC TGCCTGCCCT GCCTGTCCCT 
GAGGCCCCCA GCCCCTCCCC AGTGCCCTCT CCTGCCCCAG GCCGGAAGGG CAGCATCCAG 
GACCGGCCTC TGCCCCCACC CCCACCCCGC CTGCCTGGTT ATGGAGGCCC CAAGGTCGAG 
GGGGATCCAG AGGGCAGGGA GATGGAGGAT GACCCAGCAG GACACCACAA TGAGTACGAG 
GGCATTCCGA TGGCCGAGGA GTATGACTAT GTCCACCTGA AGGGCATGGA CAAAGCTCAG 
GGATCTAGGC CCCCGGATCA GGCCTGCACA GGGGATCCTG AACTGCCCGA GAGGGGGATG 
CCGGCGCCGC AGGAGGCCCT GTCCCCAGGG GAGCCACTGG TTGTGTCCAC CGGAGATCTG 
CAGCTCCTGT ACTTCTATGC TGGGCAATGC CAGAGCCACT ACTCAGCCCT GCAGGCAGCC 
GTGGCAGCCC TGATGTCCAG TACCCAGGCT AATCAGCCCC CGCGCCTTTT CGTGCCCCAC 
AGCAAGAGGG TGGTGGTGGC TGCTCATCGC CTGGTGTTTG TTGGGGACAC CCTGGGCCGG 
CTGGCAGCCT CTGCCCCTCT GAGAGCACAG GTCAGGGCTG CAGGTACAGC ACTGGGCCAG 
GCATTGCGGG CCACTGTGCT GGCTGTCAAG GGAGCTGCCC TGGGCTACCC ATCCAGCCCT 
GCCATCCAAG AGATGGTGCA GTGTGTAACA GAACTGGCAG GGCAGGCCCT GCAATTCACT 
ACCCTGCTCA CTAGCCTGGC TCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EFS ( NM_032459.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_115835.1 Homo sapiens embryonal Fyn-associated substrate (EFS), transcript variant 2, mRNA.

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CloneID	OHu00584
Clone ID Related Accession (Same CDS sequence)	NM_032459.3 , NM_032459.2
Accession Version	NM_032459.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1407bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-24
Organism	Homo sapiens(human)
Product	embryonal Fyn-associated substrate isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB001467.1, AB001466.1 and AA470981.1. On Mar 15, 2013 this sequence version replaced NM_032459.1. Transcript Variant: This variant (2) lacks an alternate in-frame coding exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. This isoform lacks the N-terminal SH3 domain found in isoform 1. ##Evidence-Data-START## Transcript exon combination :: AB001467.1, BC046403.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCATTG CCACGTCGGT GTATGTGGTG CCGCCCCCAG CTCGGCCCTG TCCAACCTCA 
GGACCTCCAG CTGGACCTTG CCCACCCTCT CCTGACCTCA TCTACAAAAT CCCCAGAGCT 
AGTGGGACCC AGCTGGCTGC TCCCAGAGAT GCCTTGGAGG TCTACGATGT GCCCCCCACC 
GCCCTCCGGG TGCCCTCCAG TGGCCCCTAT GACTGCCCTG CCTCCTTTTC CCACCCTCTG 
ACCCGGGTTG CCCCGCAGCC CCCTGGAGAG GATGATGCTC CCTATGATGT GCCTCTGACC 
CCAAAGCCAC CTGCAGAGCT GGAACCAGAT CTGGAGTGGG AAGGAGGCCG GGAGCCGGGG 
CCCCCCATCT ATGCTGCCCC CTCCAACCTG AAACGAGCGT CAGCCTTACT CAATTTGTAT 
GAAGCACCCG AGGAACTGCT GGCAGACGGG GAGGGCGGGG GCACTGATGA GGGGATCTAC 
GATGTGCCTC TGCTGGGGCC AGAGGCTCCC CCTTCTCCAG AGCCCCCTGG AGCCTTGGCC 
TCCCATGACC AGGACACCCT GGCCCAGCTT CTGGCCAGAA GCCCCCCACC CCCACACAGG 
CCCCGGCTCC CCTCAGCTGA GAGCCTGTCC CGCCGCCCTC TGCCTGCCCT GCCTGTCCCT 
GAGGCCCCCA GCCCCTCCCC AGTGCCCTCT CCTGCCCCAG GCCGGAAGGG CAGCATCCAG 
GACCGGCCTC TGCCCCCACC CCCACCCCGC CTGCCTGGTT ATGGAGGCCC CAAGGTCGAG 
GGGGATCCAG AGGGCAGGGA GATGGAGGAT GACCCAGCAG GACACCACAA TGAGTACGAG 
GGCATTCCGA TGGCCGAGGA GTATGACTAT GTCCACCTGA AGGGCATGGA CAAAGCTCAG 
GGATCTAGGC CCCCGGATCA GGCCTGCACA GGGGATCCTG AACTGCCCGA GAGGGGGATG 
CCGGCGCCGC AGGAGGCCCT GTCCCCAGGG GAGCCACTGG TTGTGTCCAC CGGAGATCTG 
CAGCTCCTGT ACTTCTATGC TGGGCAATGC CAGAGCCACT ACTCAGCCCT GCAGGCAGCC 
GTGGCAGCCC TGATGTCCAG TACCCAGGCT AATCAGCCCC CGCGCCTTTT CGTGCCCCAC 
AGCAAGAGGG TGGTGGTGGC TGCTCATCGC CTGGTGTTTG TTGGGGACAC CCTGGGCCGG 
CTGGCAGCCT CTGCCCCTCT GAGAGCACAG GTCAGGGCTG CAGGTACAGC ACTGGGCCAG 
GCATTGCGGG CCACTGTGCT GGCTGTCAAG GGAGCTGCCC TGGGCTACCC ATCCAGCCCT 
GCCATCCAAG AGATGGTGCA GTGTGTAACA GAACTGGCAG GGCAGGCCCT GCAATTCACT 
ACCCTGCTCA CTAGCCTGGC TCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_115835.1
CDS	609..2015
Translation	MAIATSVYVVPPPARPCPTSGPPAGPCPPSPDLIYKIPRASGTQLAAPRDALEVYDVPPTALRVPSSGPYDCPASFSHPLTRVAPQPPGEDDAPYDVPLTPKPPAELEPDLEWEGGREPGPPIYAAPSNLKRASALLNLYEAPEELLADGEGGGTDEGIYDVPLLGPEAPPSPEPPGALASHDQDTLAQLLARSPPPPHRPRLPSAESLSRRPLPALPVPEAPSPSPVPSPAPGRKGSIQDRPLPPPPPRLPGYGGPKVEGDPEGREMEDDPAGHHNEYEGIPMAEEYDYVHLKGMDKAQGSRPPDQACTGDPELPERGMPAPQEALSPGEPLVVSTGDLQLLYFYAGQCQSHYSALQAAVAALMSSTQANQPPRLFVPHSKRVVVAAHRLVFVGDTLGRLAASAPLRAQVRAAGTALGQALRATVLAVKGAALGYPSSPAIQEMVQCVTELAGQALQFTTLLTSLAP

Target ORF information:

RefSeq Version	NM_032459.2
Organism	Homo sapiens(human)
Definition	Homo sapiens embryonal Fyn-associated substrate (EFS), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_032459.2

ORF Insert Sequence:

ATGGCCATTG CCACGTCGGT GTATGTGGTG CCGCCCCCAG CTCGGCCCTG TCCAACCTCA 
GGACCTCCAG CTGGACCTTG CCCACCCTCT CCTGACCTCA TCTACAAAAT CCCCAGAGCT 
AGTGGGACCC AGCTGGCTGC TCCCAGAGAT GCCTTGGAGG TCTACGATGT GCCCCCCACC 
GCCCTCCGGG TGCCCTCCAG TGGCCCCTAT GACTGCCCTG CCTCCTTTTC CCACCCTCTG 
ACCCGGGTTG CCCCGCAGCC CCCTGGAGAG GATGATGCTC CCTATGATGT GCCTCTGACC 
CCAAAGCCAC CTGCAGAGCT GGAACCAGAT CTGGAGTGGG AAGGAGGCCG GGAGCCGGGG 
CCCCCCATCT ATGCTGCCCC CTCCAACCTG AAACGAGCGT CAGCCTTACT CAATTTGTAT 
GAAGCACCCG AGGAACTGCT GGCAGACGGG GAGGGCGGGG GCACTGATGA GGGGATCTAC 
GATGTGCCTC TGCTGGGGCC AGAGGCTCCC CCTTCTCCAG AGCCCCCTGG AGCCTTGGCC 
TCCCATGACC AGGACACCCT GGCCCAGCTT CTGGCCAGAA GCCCCCCACC CCCACACAGG 
CCCCGGCTCC CCTCAGCTGA GAGCCTGTCC CGCCGCCCTC TGCCTGCCCT GCCTGTCCCT 
GAGGCCCCCA GCCCCTCCCC AGTGCCCTCT CCTGCCCCAG GCCGGAAGGG CAGCATCCAG 
GACCGGCCTC TGCCCCCACC CCCACCCCGC CTGCCTGGTT ATGGAGGCCC CAAGGTCGAG 
GGGGATCCAG AGGGCAGGGA GATGGAGGAT GACCCAGCAG GACACCACAA TGAGTACGAG 
GGCATTCCGA TGGCCGAGGA GTATGACTAT GTCCACCTGA AGGGCATGGA CAAAGCTCAG 
GGATCTAGGC CCCCGGATCA GGCCTGCACA GGGGATCCTG AACTGCCCGA GAGGGGGATG 
CCGGCGCCGC AGGAGGCCCT GTCCCCAGGG GAGCCACTGG TTGTGTCCAC CGGAGATCTG 
CAGCTCCTGT ACTTCTATGC TGGGCAATGC CAGAGCCACT ACTCAGCCCT GCAGGCAGCC 
GTGGCAGCCC TGATGTCCAG TACCCAGGCT AATCAGCCCC CGCGCCTTTT CGTGCCCCAC 
AGCAAGAGGG TGGTGGTGGC TGCTCATCGC CTGGTGTTTG TTGGGGACAC CCTGGGCCGG 
CTGGCAGCCT CTGCCCCTCT GAGAGCACAG GTCAGGGCTG CAGGTACAGC ACTGGGCCAG 
GCATTGCGGG CCACTGTGCT GGCTGTCAAG GGAGCTGCCC TGGGCTACCC ATCCAGCCCT 
GCCATCCAAG AGATGGTGCA GTGTGTAACA GAACTGGCAG GGCAGGCCCT GCAATTCACT 
ACCCTGCTCA CTAGCCTGGC TCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EFS ( XM_005267256.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_005267313.1 Homo sapiens embryonal Fyn-associated substrate (EFS), transcript variant X1, mRNA.

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CloneID	OHu47476
Clone ID Related Accession (Same CDS sequence)	XM_005267256.1 , XM_005267256.2
Accession Version	XM_005267256.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1596bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	embryonal Fyn-associated substrate isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCATTG CCACGTCGAC CCAGCTGGCC CGGGCACTGT ATGACAACAC CGCTGAGTCC 
CCCCAGGAGC TGTCCTTCCG CCGAGGGGAT GTCCTACGGG TCCTGCAGAG AGAGGGCGCT 
GGTGGACTGG ACGGCTGGTG CCTCTGCTCC CTACACGGCC AGCAGGGCAT TGTGCCCGCC 
AACAGGGTGA AGCTCTTGCC TGCTGGCCCA GCACCCAAGC CCAGCCTCTC TCCTGCGTCC 
CCAGCCCAGC CTGGCTCACC ATATCCAGCC CCAGATCACA GCAATGAGGA CCAGGAGGTG 
TATGTGGTGC CGCCCCCAGC TCGGCCCTGT CCAACCTCAG GACCTCCAGC TGGACCTTGC 
CCACCCTCTC CTGACCTCAT CTACAAAATC CCCAGAGCTA GTGGGACCCA GCTGGCTGCT 
CCCAGAGATG CCTTGGAGGT CTACGATGTG CCCCCCACCG CCCTCCGGGT GCCCTCCAGT 
GGCCCCTATG ACTGCCCTGC CTCCTTTTCC CACCCTCTGA CCCGGGTTGC CCCGCAGCCC 
CCTGGAGAGG ATGATGCTCC CTATGATGTG CCTCTGACCC CAAAGCCACC TGCAGAGCTG 
GAACCAGATC TGGAGTGGGA AGGAGGCCGG GAGCCGGGGC CCCCCATCTA TGCTGCCCCC 
TCCAACCTGA AACGAGCGTC AGCCTTACTC AATTTGTATG AAGCACCCGA GGAACTGCTG 
GCAGACGGGG AGGGCGGGGG CACTGATGAG GGGATCTACG ATGTGCCTCT GCTGGGGCCA 
GAGGCTCCCC CTTCTCCAGA GCCCCCTGGA GCCTTGGCCT CCCATGACCA GGACACCCTG 
GCCCAGCTTC TGGCCAGAAG CCCCCCACCC CCACACAGGC CCCGGCTCCC CTCAGCTGAG 
AGCCTGTCCC GCCGCCCTCT GCCTGCCCTG CCTGTCCCTG AGGCCCCCAG CCCCTCCCCA 
GTGCCCTCTC CTGCCCCAGG CCGGAAGGGC AGCATCCAGG ACCGGCCTCT GCCCCCACCC 
CCACCCCGCC TGCCTGGTTA TGGAGGCCCC AAGGTCGAGG GGGATCCAGA GGGCAGGGAG 
ATGGAGGATG ACCCAGCAGG ACACCACAAT GAGTACGAGG GCATTCCGAT GGCCGAGGAG 
TATGACTATG TCCACCTGAA GGAGGCCCTG TCCCCAGGGG AGCCACTGGT TGTGTCCACC 
GGAGATCTGC AGCTCCTGTA CTTCTATGCT GGGCAATGCC AGAGCCACTA CTCAGCCCTG 
CAGGCAGCCG TGGCAGCCCT GATGTCCAGT ACCCAGGCTA ATCAGCCCCC GCGCCTTTTC 
GTGCCCCACA GCAAGAGGGT GGTGGTGGCT GCTCATCGCC TGGTGTTTGT TGGGGACACC 
CTGGGCCGGC TGGCAGCCTC TGCCCCTCTG AGAGCACAGG TCAGGGCTGC AGGTACAGCA 
CTGGGCCAGG CATTGCGGGC CACTGTGCTG GCTGTCAAGG GAGCTGCCCT GGGCTACCCA 
TCCAGCCCTG CCATCCAAGA GATGGTGCAG TGTGTAACAG AACTGGCAGG GCAGGCCCTG 
CAATTCACTA CCCTGCTCAC TAGCCTGGCT CCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005267313.1
CDS	190..1785
Translation	MAIATSTQLARALYDNTAESPQELSFRRGDVLRVLQREGAGGLDGWCLCSLHGQQGIVPANRVKLLPAGPAPKPSLSPASPAQPGSPYPAPDHSNEDQEVYVVPPPARPCPTSGPPAGPCPPSPDLIYKIPRASGTQLAAPRDALEVYDVPPTALRVPSSGPYDCPASFSHPLTRVAPQPPGEDDAPYDVPLTPKPPAELEPDLEWEGGREPGPPIYAAPSNLKRASALLNLYEAPEELLADGEGGGTDEGIYDVPLLGPEAPPSPEPPGALASHDQDTLAQLLARSPPPPHRPRLPSAESLSRRPLPALPVPEAPSPSPVPSPAPGRKGSIQDRPLPPPPPRLPGYGGPKVEGDPEGREMEDDPAGHHNEYEGIPMAEEYDYVHLKEALSPGEPLVVSTGDLQLLYFYAGQCQSHYSALQAAVAALMSSTQANQPPRLFVPHSKRVVVAAHRLVFVGDTLGRLAASAPLRAQVRAAGTALGQALRATVLAVKGAALGYPSSPAIQEMVQCVTELAGQALQFTTLLTSLAP

Target ORF information:

RefSeq Version	XM_005267256.1
Organism	Homo sapiens(human)
Definition	Homo sapiens embryonal Fyn-associated substrate (EFS), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005267256.1

ORF Insert Sequence:

ATGGCCATTG CCACGTCGAC CCAGCTGGCC CGGGCACTGT ATGACAACAC CGCTGAGTCC 
CCCCAGGAGC TGTCCTTCCG CCGAGGGGAT GTCCTACGGG TCCTGCAGAG AGAGGGCGCT 
GGTGGACTGG ACGGCTGGTG CCTCTGCTCC CTACACGGCC AGCAGGGCAT TGTGCCCGCC 
AACAGGGTGA AGCTCTTGCC TGCTGGCCCA GCACCCAAGC CCAGCCTCTC TCCTGCGTCC 
CCAGCCCAGC CTGGCTCACC ATATCCAGCC CCAGATCACA GCAATGAGGA CCAGGAGGTG 
TATGTGGTGC CGCCCCCAGC TCGGCCCTGT CCAACCTCAG GACCTCCAGC TGGACCTTGC 
CCACCCTCTC CTGACCTCAT CTACAAAATC CCCAGAGCTA GTGGGACCCA GCTGGCTGCT 
CCCAGAGATG CCTTGGAGGT CTACGATGTG CCCCCCACCG CCCTCCGGGT GCCCTCCAGT 
GGCCCCTATG ACTGCCCTGC CTCCTTTTCC CACCCTCTGA CCCGGGTTGC CCCGCAGCCC 
CCTGGAGAGG ATGATGCTCC CTATGATGTG CCTCTGACCC CAAAGCCACC TGCAGAGCTG 
GAACCAGATC TGGAGTGGGA AGGAGGCCGG GAGCCGGGGC CCCCCATCTA TGCTGCCCCC 
TCCAACCTGA AACGAGCGTC AGCCTTACTC AATTTGTATG AAGCACCCGA GGAACTGCTG 
GCAGACGGGG AGGGCGGGGG CACTGATGAG GGGATCTACG ATGTGCCTCT GCTGGGGCCA 
GAGGCTCCCC CTTCTCCAGA GCCCCCTGGA GCCTTGGCCT CCCATGACCA GGACACCCTG 
GCCCAGCTTC TGGCCAGAAG CCCCCCACCC CCACACAGGC CCCGGCTCCC CTCAGCTGAG 
AGCCTGTCCC GCCGCCCTCT GCCTGCCCTG CCTGTCCCTG AGGCCCCCAG CCCCTCCCCA 
GTGCCCTCTC CTGCCCCAGG CCGGAAGGGC AGCATCCAGG ACCGGCCTCT GCCCCCACCC 
CCACCCCGCC TGCCTGGTTA TGGAGGCCCC AAGGTCGAGG GGGATCCAGA GGGCAGGGAG 
ATGGAGGATG ACCCAGCAGG ACACCACAAT GAGTACGAGG GCATTCCGAT GGCCGAGGAG 
TATGACTATG TCCACCTGAA GGAGGCCCTG TCCCCAGGGG AGCCACTGGT TGTGTCCACC 
GGAGATCTGC AGCTCCTGTA CTTCTATGCT GGGCAATGCC AGAGCCACTA CTCAGCCCTG 
CAGGCAGCCG TGGCAGCCCT GATGTCCAGT ACCCAGGCTA ATCAGCCCCC GCGCCTTTTC 
GTGCCCCACA GCAAGAGGGT GGTGGTGGCT GCTCATCGCC TGGTGTTTGT TGGGGACACC 
CTGGGCCGGC TGGCAGCCTC TGCCCCTCTG AGAGCACAGG TCAGGGCTGC AGGTACAGCA 
CTGGGCCAGG CATTGCGGGC CACTGTGCTG GCTGTCAAGG GAGCTGCCCT GGGCTACCCA 
TCCAGCCCTG CCATCCAAGA GATGGTGCAG TGTGTAACAG AACTGGCAGG GCAGGCCCTG 
CAATTCACTA CCCTGCTCAC TAGCCTGGCT CCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EFS ( XM_005267256.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_005267313.1 Homo sapiens embryonal Fyn-associated substrate (EFS), transcript variant X1, mRNA.

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CloneID	OHu47476
Clone ID Related Accession (Same CDS sequence)	XM_005267256.1 , XM_005267256.2
Accession Version	XM_005267256.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1596bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	embryonal Fyn-associated substrate isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005267256.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCATTG CCACGTCGAC CCAGCTGGCC CGGGCACTGT ATGACAACAC CGCTGAGTCC 
CCCCAGGAGC TGTCCTTCCG CCGAGGGGAT GTCCTACGGG TCCTGCAGAG AGAGGGCGCT 
GGTGGACTGG ACGGCTGGTG CCTCTGCTCC CTACACGGCC AGCAGGGCAT TGTGCCCGCC 
AACAGGGTGA AGCTCTTGCC TGCTGGCCCA GCACCCAAGC CCAGCCTCTC TCCTGCGTCC 
CCAGCCCAGC CTGGCTCACC ATATCCAGCC CCAGATCACA GCAATGAGGA CCAGGAGGTG 
TATGTGGTGC CGCCCCCAGC TCGGCCCTGT CCAACCTCAG GACCTCCAGC TGGACCTTGC 
CCACCCTCTC CTGACCTCAT CTACAAAATC CCCAGAGCTA GTGGGACCCA GCTGGCTGCT 
CCCAGAGATG CCTTGGAGGT CTACGATGTG CCCCCCACCG CCCTCCGGGT GCCCTCCAGT 
GGCCCCTATG ACTGCCCTGC CTCCTTTTCC CACCCTCTGA CCCGGGTTGC CCCGCAGCCC 
CCTGGAGAGG ATGATGCTCC CTATGATGTG CCTCTGACCC CAAAGCCACC TGCAGAGCTG 
GAACCAGATC TGGAGTGGGA AGGAGGCCGG GAGCCGGGGC CCCCCATCTA TGCTGCCCCC 
TCCAACCTGA AACGAGCGTC AGCCTTACTC AATTTGTATG AAGCACCCGA GGAACTGCTG 
GCAGACGGGG AGGGCGGGGG CACTGATGAG GGGATCTACG ATGTGCCTCT GCTGGGGCCA 
GAGGCTCCCC CTTCTCCAGA GCCCCCTGGA GCCTTGGCCT CCCATGACCA GGACACCCTG 
GCCCAGCTTC TGGCCAGAAG CCCCCCACCC CCACACAGGC CCCGGCTCCC CTCAGCTGAG 
AGCCTGTCCC GCCGCCCTCT GCCTGCCCTG CCTGTCCCTG AGGCCCCCAG CCCCTCCCCA 
GTGCCCTCTC CTGCCCCAGG CCGGAAGGGC AGCATCCAGG ACCGGCCTCT GCCCCCACCC 
CCACCCCGCC TGCCTGGTTA TGGAGGCCCC AAGGTCGAGG GGGATCCAGA GGGCAGGGAG 
ATGGAGGATG ACCCAGCAGG ACACCACAAT GAGTACGAGG GCATTCCGAT GGCCGAGGAG 
TATGACTATG TCCACCTGAA GGAGGCCCTG TCCCCAGGGG AGCCACTGGT TGTGTCCACC 
GGAGATCTGC AGCTCCTGTA CTTCTATGCT GGGCAATGCC AGAGCCACTA CTCAGCCCTG 
CAGGCAGCCG TGGCAGCCCT GATGTCCAGT ACCCAGGCTA ATCAGCCCCC GCGCCTTTTC 
GTGCCCCACA GCAAGAGGGT GGTGGTGGCT GCTCATCGCC TGGTGTTTGT TGGGGACACC 
CTGGGCCGGC TGGCAGCCTC TGCCCCTCTG AGAGCACAGG TCAGGGCTGC AGGTACAGCA 
CTGGGCCAGG CATTGCGGGC CACTGTGCTG GCTGTCAAGG GAGCTGCCCT GGGCTACCCA 
TCCAGCCCTG CCATCCAAGA GATGGTGCAG TGTGTAACAG AACTGGCAGG GCAGGCCCTG 
CAATTCACTA CCCTGCTCAC TAGCCTGGCT CCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005267313.1
CDS	183..1778
Translation	MAIATSTQLARALYDNTAESPQELSFRRGDVLRVLQREGAGGLDGWCLCSLHGQQGIVPANRVKLLPAGPAPKPSLSPASPAQPGSPYPAPDHSNEDQEVYVVPPPARPCPTSGPPAGPCPPSPDLIYKIPRASGTQLAAPRDALEVYDVPPTALRVPSSGPYDCPASFSHPLTRVAPQPPGEDDAPYDVPLTPKPPAELEPDLEWEGGREPGPPIYAAPSNLKRASALLNLYEAPEELLADGEGGGTDEGIYDVPLLGPEAPPSPEPPGALASHDQDTLAQLLARSPPPPHRPRLPSAESLSRRPLPALPVPEAPSPSPVPSPAPGRKGSIQDRPLPPPPPRLPGYGGPKVEGDPEGREMEDDPAGHHNEYEGIPMAEEYDYVHLKEALSPGEPLVVSTGDLQLLYFYAGQCQSHYSALQAAVAALMSSTQANQPPRLFVPHSKRVVVAAHRLVFVGDTLGRLAASAPLRAQVRAAGTALGQALRATVLAVKGAALGYPSSPAIQEMVQCVTELAGQALQFTTLLTSLAP

Target ORF information:

RefSeq Version	XM_005267256.2
Organism	Homo sapiens(human)
Definition	Homo sapiens embryonal Fyn-associated substrate (EFS), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005267256.2

ORF Insert Sequence:

ATGGCCATTG CCACGTCGAC CCAGCTGGCC CGGGCACTGT ATGACAACAC CGCTGAGTCC 
CCCCAGGAGC TGTCCTTCCG CCGAGGGGAT GTCCTACGGG TCCTGCAGAG AGAGGGCGCT 
GGTGGACTGG ACGGCTGGTG CCTCTGCTCC CTACACGGCC AGCAGGGCAT TGTGCCCGCC 
AACAGGGTGA AGCTCTTGCC TGCTGGCCCA GCACCCAAGC CCAGCCTCTC TCCTGCGTCC 
CCAGCCCAGC CTGGCTCACC ATATCCAGCC CCAGATCACA GCAATGAGGA CCAGGAGGTG 
TATGTGGTGC CGCCCCCAGC TCGGCCCTGT CCAACCTCAG GACCTCCAGC TGGACCTTGC 
CCACCCTCTC CTGACCTCAT CTACAAAATC CCCAGAGCTA GTGGGACCCA GCTGGCTGCT 
CCCAGAGATG CCTTGGAGGT CTACGATGTG CCCCCCACCG CCCTCCGGGT GCCCTCCAGT 
GGCCCCTATG ACTGCCCTGC CTCCTTTTCC CACCCTCTGA CCCGGGTTGC CCCGCAGCCC 
CCTGGAGAGG ATGATGCTCC CTATGATGTG CCTCTGACCC CAAAGCCACC TGCAGAGCTG 
GAACCAGATC TGGAGTGGGA AGGAGGCCGG GAGCCGGGGC CCCCCATCTA TGCTGCCCCC 
TCCAACCTGA AACGAGCGTC AGCCTTACTC AATTTGTATG AAGCACCCGA GGAACTGCTG 
GCAGACGGGG AGGGCGGGGG CACTGATGAG GGGATCTACG ATGTGCCTCT GCTGGGGCCA 
GAGGCTCCCC CTTCTCCAGA GCCCCCTGGA GCCTTGGCCT CCCATGACCA GGACACCCTG 
GCCCAGCTTC TGGCCAGAAG CCCCCCACCC CCACACAGGC CCCGGCTCCC CTCAGCTGAG 
AGCCTGTCCC GCCGCCCTCT GCCTGCCCTG CCTGTCCCTG AGGCCCCCAG CCCCTCCCCA 
GTGCCCTCTC CTGCCCCAGG CCGGAAGGGC AGCATCCAGG ACCGGCCTCT GCCCCCACCC 
CCACCCCGCC TGCCTGGTTA TGGAGGCCCC AAGGTCGAGG GGGATCCAGA GGGCAGGGAG 
ATGGAGGATG ACCCAGCAGG ACACCACAAT GAGTACGAGG GCATTCCGAT GGCCGAGGAG 
TATGACTATG TCCACCTGAA GGAGGCCCTG TCCCCAGGGG AGCCACTGGT TGTGTCCACC 
GGAGATCTGC AGCTCCTGTA CTTCTATGCT GGGCAATGCC AGAGCCACTA CTCAGCCCTG 
CAGGCAGCCG TGGCAGCCCT GATGTCCAGT ACCCAGGCTA ATCAGCCCCC GCGCCTTTTC 
GTGCCCCACA GCAAGAGGGT GGTGGTGGCT GCTCATCGCC TGGTGTTTGT TGGGGACACC 
CTGGGCCGGC TGGCAGCCTC TGCCCCTCTG AGAGCACAGG TCAGGGCTGC AGGTACAGCA 
CTGGGCCAGG CATTGCGGGC CACTGTGCTG GCTGTCAAGG GAGCTGCCCT GGGCTACCCA 
TCCAGCCCTG CCATCCAAGA GATGGTGCAG TGTGTAACAG AACTGGCAGG GCAGGCCCTG 
CAATTCACTA CCCTGCTCAC TAGCCTGGCT CCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EFS ( XM_024449457.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024305225.1 Homo sapiens embryonal Fyn-associated substrate (EFS), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu103854
Clone ID Related Accession (Same CDS sequence)	XM_024449457.1
Accession Version	XM_024449457.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1317bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	embryonal Fyn-associated substrate isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCATTG CCACGTCGGT GTATGTGGTG CCGCCCCCAG CTCGGCCCTG TCCAACCTCA 
GGACCTCCAG CTGGACCTTG CCCACCCTCT CCTGACCTCA TCTACAAAAT CCCCAGAGCT 
AGTGGGACCC AGCTGGCTGC TCCCAGAGAT GCCTTGGAGG TCTACGATGT GCCCCCCACC 
GCCCTCCGGG TGCCCTCCAG TGGCCCCTAT GACTGCCCTG CCTCCTTTTC CCACCCTCTG 
ACCCGGGTTG CCCCGCAGCC CCCTGGAGAG GATGATGCTC CCTATGATGT GCCTCTGACC 
CCAAAGCCAC CTGCAGAGCT GGAACCAGAT CTGGAGTGGG AAGGAGGCCG GGAGCCGGGG 
CCCCCCATCT ATGCTGCCCC CTCCAACCTG AAACGAGCGT CAGCCTTACT CAATTTGTAT 
GAAGCACCCG AGGAACTGCT GGCAGACGGG GAGGGCGGGG GCACTGATGA GGGGATCTAC 
GATGTGCCTC TGCTGGGGCC AGAGGCTCCC CCTTCTCCAG AGCCCCCTGG AGCCTTGGCC 
TCCCATGACC AGGACACCCT GGCCCAGCTT CTGGCCAGAA GCCCCCCACC CCCACACAGG 
CCCCGGCTCC CCTCAGCTGA GAGCCTGTCC CGCCGCCCTC TGCCTGCCCT GCCTGTCCCT 
GAGGCCCCCA GCCCCTCCCC AGTGCCCTCT CCTGCCCCAG GCCGGAAGGG CAGCATCCAG 
GACCGGCCTC TGCCCCCACC CCCACCCCGC CTGCCTGGTT ATGGAGGCCC CAAGGTCGAG 
GGGGATCCAG AGGGCAGGGA GATGGAGGAT GACCCAGCAG GACACCACAA TGAGTACGAG 
GGCATTCCGA TGGCCGAGGA GTATGACTAT GTCCACCTGA AGGAGGCCCT GTCCCCAGGG 
GAGCCACTGG TTGTGTCCAC CGGAGATCTG CAGCTCCTGT ACTTCTATGC TGGGCAATGC 
CAGAGCCACT ACTCAGCCCT GCAGGCAGCC GTGGCAGCCC TGATGTCCAG TACCCAGGCT 
AATCAGCCCC CGCGCCTTTT CGTGCCCCAC AGCAAGAGGG TGGTGGTGGC TGCTCATCGC 
CTGGTGTTTG TTGGGGACAC CCTGGGCCGG CTGGCAGCCT CTGCCCCTCT GAGAGCACAG 
GTCAGGGCTG CAGGTACAGC ACTGGGCCAG GCATTGCGGG CCACTGTGCT GGCTGTCAAG 
GGAGCTGCCC TGGGCTACCC ATCCAGCCCT GCCATCCAAG AGATGGTGCA GTGTGTAACA 
GAACTGGCAG GGCAGGCCCT GCAATTCACT ACCCTGCTCA CTAGCCTGGC TCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024305225.1
CDS	183..1499
Translation	MAIATSVYVVPPPARPCPTSGPPAGPCPPSPDLIYKIPRASGTQLAAPRDALEVYDVPPTALRVPSSGPYDCPASFSHPLTRVAPQPPGEDDAPYDVPLTPKPPAELEPDLEWEGGREPGPPIYAAPSNLKRASALLNLYEAPEELLADGEGGGTDEGIYDVPLLGPEAPPSPEPPGALASHDQDTLAQLLARSPPPPHRPRLPSAESLSRRPLPALPVPEAPSPSPVPSPAPGRKGSIQDRPLPPPPPRLPGYGGPKVEGDPEGREMEDDPAGHHNEYEGIPMAEEYDYVHLKEALSPGEPLVVSTGDLQLLYFYAGQCQSHYSALQAAVAALMSSTQANQPPRLFVPHSKRVVVAAHRLVFVGDTLGRLAASAPLRAQVRAAGTALGQALRATVLAVKGAALGYPSSPAIQEMVQCVTELAGQALQFTTLLTSLAP

Target ORF information:

RefSeq Version	XM_024449457.1
Organism	Homo sapiens(human)
Definition	Homo sapiens embryonal Fyn-associated substrate (EFS), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024449457.1

ORF Insert Sequence:

ATGGCCATTG CCACGTCGGT GTATGTGGTG CCGCCCCCAG CTCGGCCCTG TCCAACCTCA 
GGACCTCCAG CTGGACCTTG CCCACCCTCT CCTGACCTCA TCTACAAAAT CCCCAGAGCT 
AGTGGGACCC AGCTGGCTGC TCCCAGAGAT GCCTTGGAGG TCTACGATGT GCCCCCCACC 
GCCCTCCGGG TGCCCTCCAG TGGCCCCTAT GACTGCCCTG CCTCCTTTTC CCACCCTCTG 
ACCCGGGTTG CCCCGCAGCC CCCTGGAGAG GATGATGCTC CCTATGATGT GCCTCTGACC 
CCAAAGCCAC CTGCAGAGCT GGAACCAGAT CTGGAGTGGG AAGGAGGCCG GGAGCCGGGG 
CCCCCCATCT ATGCTGCCCC CTCCAACCTG AAACGAGCGT CAGCCTTACT CAATTTGTAT 
GAAGCACCCG AGGAACTGCT GGCAGACGGG GAGGGCGGGG GCACTGATGA GGGGATCTAC 
GATGTGCCTC TGCTGGGGCC AGAGGCTCCC CCTTCTCCAG AGCCCCCTGG AGCCTTGGCC 
TCCCATGACC AGGACACCCT GGCCCAGCTT CTGGCCAGAA GCCCCCCACC CCCACACAGG 
CCCCGGCTCC CCTCAGCTGA GAGCCTGTCC CGCCGCCCTC TGCCTGCCCT GCCTGTCCCT 
GAGGCCCCCA GCCCCTCCCC AGTGCCCTCT CCTGCCCCAG GCCGGAAGGG CAGCATCCAG 
GACCGGCCTC TGCCCCCACC CCCACCCCGC CTGCCTGGTT ATGGAGGCCC CAAGGTCGAG 
GGGGATCCAG AGGGCAGGGA GATGGAGGAT GACCCAGCAG GACACCACAA TGAGTACGAG 
GGCATTCCGA TGGCCGAGGA GTATGACTAT GTCCACCTGA AGGAGGCCCT GTCCCCAGGG 
GAGCCACTGG TTGTGTCCAC CGGAGATCTG CAGCTCCTGT ACTTCTATGC TGGGCAATGC 
CAGAGCCACT ACTCAGCCCT GCAGGCAGCC GTGGCAGCCC TGATGTCCAG TACCCAGGCT 
AATCAGCCCC CGCGCCTTTT CGTGCCCCAC AGCAAGAGGG TGGTGGTGGC TGCTCATCGC 
CTGGTGTTTG TTGGGGACAC CCTGGGCCGG CTGGCAGCCT CTGCCCCTCT GAGAGCACAG 
GTCAGGGCTG CAGGTACAGC ACTGGGCCAG GCATTGCGGG CCACTGTGCT GGCTGTCAAG 
GGAGCTGCCC TGGGCTACCC ATCCAGCCCT GCCATCCAAG AGATGGTGCA GTGTGTAACA 
GAACTGGCAG GGCAGGCCCT GCAATTCACT ACCCTGCTCA CTAGCCTGGC TCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Embryonal Fyn-associated substrate (EFS) and CASS4: The lesser-known CAS protein family members.
Gene570(1)25-35(2015 Oct)
Deneka A,Korobeynikov V,Golemis EA

Decreased expression of EFS is correlated with the advanced prostate cancer.
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine36(2)799-805(2015 Feb)
Sertkaya S,Hamid SM,Dilsiz N,Varisli L

EFS shows biallelic methylation in uveal melanoma with poor prognosis as well as tissue-specific methylation.
BMC cancer11380(2011 Aug)
Neumann LC,Weinh?usel A,Thomas S,Horsthemke B,Lohmann DR,Zeschnigk M

New genetic associations detected in a host response study to hepatitis B vaccine.
Genes and immunity11(3)232-8(2010 Apr)
Davila S,Froeling FE,Tan A,Bonnard C,Boland GJ,Snippe H,Hibberd ML,Seielstad M

Systematic identification of SH3 domain-mediated human protein-protein interactions by peptide array target screening.
Proteomics7(11)1775-85(2007 Jun)
Wu C,Ma MH,Brown KR,Geisler M,Li L,Tzeng E,Jia CY,Jurisica I,Li SS

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EFS cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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