STAG1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	STAG1
Entrez Gene ID	10274
Full Name	stromal antigen 1
Synonyms	MRD47,SA1,SCC3A
General protein information	Preferred Names stromal antigen 1 Names cohesin subunit SA-1 SCC3 homolog 1 nuclear protein stromal antigen 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 3 Map Location: 3q22.3
Summary	This gene is a member of the SCC3 family and is expressed in the nucleus. It encodes a component of cohesin, a multisubunit protein complex that provides sister chromatid cohesion along the length of a chromosome from DNA replication through prophase and prometaphase, after which it is dissociated in preparation for segregation during anaphase. [provided by RefSeq, Jul 2008].
Disorder MIM:	604358
Disorder Html:	Mental retardation, autosomal dominant 47, 617635 (3)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011512329.2	XP_011510631.1	cohesin subunit SA-1 isoform X1
XM_011512331.2	XP_011510633.1	cohesin subunit SA-1 isoform X1
XM_017005524.1	XP_016861013.1	cohesin subunit SA-1 isoform X2
NM_005862.2	NP_005853.2	cohesin subunit SA-1
XM_017005525.1	XP_016861014.1	cohesin subunit SA-1 isoform X3
NM_005862.3	NP_005853.2	cohesin subunit SA-1
XM_017005523.1	XP_016861012.1	cohesin subunit SA-1 isoform X1
XM_017005524.2	XP_016861013.1	cohesin subunit SA-1 isoform X2

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	STAG1	XP_005634521.1
Rattus norvegicus (Norway rat)	Stag1	NP_001101649.1
Pan troglodytes (chimpanzee)	STAG1	XP_001154214.1
Danio rerio (zebrafish)	stag1a	XP_692954.2
Oryza sativa (rice)	Os05g0188500	NP_001054841.1
Mus musculus (house mouse)	Stag1	NP_033308.2
Danio rerio (zebrafish)	LOC563669	XP_692120.3
Drosophila melanogaster (fruit fly)	SA	NP_477268.2
Arabidopsis thaliana (thale cress)	SCC3	NP_566119.1
Xenopus tropicalis (tropical clawed frog)	LOC100158523	NP_001121432.1
Homo sapiens (human)	STAG1	NP_005853.2
Bos taurus (cattle)	STAG1	XP_002685098.1
Gallus gallus (chicken)	STAG1	XP_422558.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006998	XP_308771.3

Gene Ontology
Bibliography

Related articles in PubMed

Synthetic lethality between the cohesin subunits
van der Lelij P, Lieb S, Jude J, Wutz G, Santos CP, Falkenberg K, Schlattl A, Ban J, Schwentner R, Hoffmann T, Kovar H, Real FX, Waldman T, Pearson MA, Kraut N, Peters JM, Zuber J, Petronczki M
eLife6(2017 Jul)

Synthetic lethal interaction between the tumour suppressor STAG2 and its paralog STAG1.
Benedetti L, Cereda M, Monteverde L, Desai N, Ciccarelli FD
Oncotarget8(23)37619-37632(2017 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

TRF1 tethers SA1 within telomeric regions that SA1 transiently interacts with. SA1 and TRF1 together form longer DNA-DNA pairing tracts than with TRF1 alone, as revealed by atomic force microscopy imaging
Title: Functional interplay between SA1 and TRF1 in telomeric DNA binding and DNA-DNA pairing.

Data support the hypothesis that CTCF and cohesin SA-1 have multiple roles in the regulation of gene expression during erythropoiesis.
Title: CTCF and CohesinSA-1 Mark Active Promoters and Boundaries of Repressive Chromatin Domains in Primary Human Erythroid Cells.

cohesin-SA1 and cohesin-SA2 participate in the DNA damage response
Title: Distinct functions of human cohesin-SA1 and cohesin-SA2 in double-strand break repair.

Mutations in STAG1 is associated with acute myeloid leukemia.
Title: Mutations in the cohesin complex in acute myeloid leukemia: clinical and prognostic implications.

Rad21 binds to SA proteins through two SA-binding motifs on Rad21.
Title: Characterization of the interaction between the cohesin subunits Rad21 and SA1/2.

The following STAG1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the STAG1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu93732	XM_017005525.1 Latest version!	Homo sapiens stromal antigen 1 (STAG1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	10-12	$391.30 $559.00
OHu63831	XM_011512329.2 Latest version!	Homo sapiens stromal antigen 1 (STAG1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$559.30 $799.00
OHu63831	XM_011512331.2 Latest version!	Homo sapiens stromal antigen 1 (STAG1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$559.30 $799.00
OHu63831	XM_017005523.1 Latest version!	Homo sapiens stromal antigen 1 (STAG1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$559.30 $799.00
OHu63832	XM_017005524.2 Latest version!	Homo sapiens stromal antigen 1 (STAG1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$559.30 $799.00
OHu15240	NM_005862.3 Latest version!	Homo sapiens stromal antigen 1 (STAG1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
View Old Accession Versions >>
OHu15240	NM_005862.2	Homo sapiens stromal antigen 1 (STAG1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu63832	XM_017005524.1	Homo sapiens stromal antigen 1 (STAG1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$559.30 $799.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

STAG1 ( XM_017005525.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016861014.1 Homo sapiens stromal antigen 1 (STAG1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OHu93732
Clone ID Related Accession (Same CDS sequence)	XM_017005525.1
Accession Version	XM_017005525.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2466bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	cohesin subunit SA-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTATTTA GCAGACATGA CCCACAAGCA GAAGAAGCAT TAGCAAAGAG GAGGGGAAGA 
AACAGCCCGA ATGGAAACCT CATTAGGATG CTGGTTCTTT TCTTTCTTGA AAGTGAGTTA 
CATGAACATG CAGCCTACTT GGTGGACAGT TTATGGGAGA GCTCTCAAGA ACTGTTGAAA 
GACTGGGAAT GTATGACAGA GTTGCTATTA GAAGAACCTG TTCAAGGAGA GGAAGCAATG 
TCTGATCGTC AAGAGAGTGC TCTTATAGAG CTAATGGTTT GTACAATTCG TCAAGCTGCT 
GAGGCACATC CTCCAGTGGG AAGGGGTACC GGCAAGAGAG TGCTAACTGC CAAAGAAAGG 
AAAACTCAAA TTGATGATAG AAACAAATTG ACTGAACATT TTATTATTAC ACTTCCTATG 
TTACTGTCAA AGTATTCTGC AGATGCAGAG AAGGTAGCAA ACTTGCTACA AATCCCACAG 
TATTTTGATT TAGAAATCTA CAGCACAGGT AGAATGGAAA AGCATCTGGA TGCTTTATTA 
AAACAGATTA AGTTTGTTGT GGAGAAACAC GTAGAATCAG ATGTTCTAGA AGCCTGCAGT 
AAAACCTATA GTATCTTATG CAGTGAAGAA TATACCATCC AGAACAGAGT TGACATAGCT 
CGAAGCCAGC TGATTGATGA GTTTGTAGAT CGATTCAATC ATTCTGTGGA AGACCTATTG 
CAAGAGGGAG AAGAAGCTGA TGATGATGAC ATTTACAATG TTCTTTCTAC ATTAAAGCGG 
TTAACTTCTT TTCACAATGC ACATGATCTC ACAAAATGGG ATCTCTTTGG TAATTGCTAC 
AGATTATTGA AGACTGGAAT TGAACATGGA GCCATGCCAG AACAGATAGT CGTGCAAGCA 
CTGCAGTGTT CCCATTATTC GATTCTTTGG CAGTTGGTGA AAATTACTGA TGGCTCTCCT 
TCCAAAGAGG ATTTGTTGGT ATTGAGGAAA ACGGTGAAAT CCTTTTTGGC TGTTTGCCAG 
CAGTGCCTGT CTAATGTTAA TACTCCAGTG AAAGAACAGG CTTTCATGTT ACTCTGTGAT 
CTTCTGATGA TTTTCAGCCA CCAATTAATG ACAGGTGGCA GAGAGGGCCT TCAGCCTTTG 
GTGTTCAATC CAGATACTGG ACTCCAATCT GAACTCCTCA GTTTTGTGAT GGATCACGTT 
TTTATTGACC AAGACGAGGA GAACCAGAGC ATGGAGGGTG ATGAAGAAGA TGAAGCTAAT 
AAAATTGAGG CCTTACATAA AAGAAGGAAT CTACTTGCTG CTTTCAGCAA ACTTATCATT 
TATGACATTG TTGACATGCA TGCAGCTGCA GACATCTTCA AACACTACAT GAAGTATTAC 
AATGACTATG GTGATATTAT TAAGGAAACA CTGAGTAAAA CCAGGCAGAT TGATAAAATT 
CAGTGTGCCA AGACTCTCAT TCTCAGTTTG CAACAGTTAT TTAATGAACT TGTTCAAGAG 
CAAGGTCCCA ACCTAGATAG GACATCTGCC CATGTCAGTG GCATTAAAGA ACTGGCACGT 
CGCTTTGCCC TTACATTTGG ATTGGACCAG ATTAAGACAC GAGAAGCAGT TGCCACACTT 
CACAAGGATG GCATAGAGTT TGCATTTAAA TACCAAAATC AGAAAGGACA AGAGTATCCA 
CCTCCTAATC TGGCTTTTCT TGAAGTACTA AGTGAATTTT CTTCTAAACT TCTTCGACAG 
GACAAAAAGA CAGTTCATTC ATACCTAGAG AAATTCCTTA CCGAGCAGAT GATGGAAAGG 
AGGGAGGATG TATGGCTTCC ACTCATCTCC TATAGAAATT CATTAGTCAC TGGGGGTGAA 
GATGATAGAA TGTCTGTGAA CAGTGGAAGT AGCAGCAGCA AAACCTCATC AGTAAGGAAT 
AAGAAAGGAC GACCTCCACT TCATAAAAAA CGAGTAGAAG ATGAGAGTCT GGATAACACA 
TGGCTAAACA GGACTGACAC CATGATTCAG ACTCCTGGCC CCCTGCCAGC ACCACAACTC 
ACATCCACTG TACTGCGGGA GAACAGTCGG CCCATGGGAG ACCAGATTCA AGAACCTGAG 
TCTGAACATG GTTCTGAACC AGACTTTTTA CACAATCCTC AGATGCAGAT CTCTTGGTTA 
GGCCAGCCGA AGTTAGAAGA CTTAAATCGG AAGGACAGAA CAGGAATGAA CTACATGAAA 
GTGAGAACTG GAGTGAGGCA TGCTGTTCGG GGTCTAATGG AGGAAGATGC TGAGCCCATC 
TTTGAAGATG TGATGATGTC ATCCCGAAGC CAGTTAGAAG ATATGAATGA AGAATTTGAG 
GACACCATGG TTATTGATCT GCCTCCATCA AGAAATCGGC GAGAGAGAGC TGAGCTAAGG 
CCAGACTTCT TTGACTCTGC AGCTATCATA GAAGATGATT CAGGATTTGG AATGCCTATG 
TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016861014.1
CDS	176..2641
Translation	MLFSRHDPQAEEALAKRRGRNSPNGNLIRMLVLFFLESELHEHAAYLVDSLWESSQELLKDWECMTELLLEEPVQGEEAMSDRQESALIELMVCTIRQAAEAHPPVGRGTGKRVLTAKERKTQIDDRNKLTEHFIITLPMLLSKYSADAEKVANLLQIPQYFDLEIYSTGRMEKHLDALLKQIKFVVEKHVESDVLEACSKTYSILCSEEYTIQNRVDIARSQLIDEFVDRFNHSVEDLLQEGEEADDDDIYNVLSTLKRLTSFHNAHDLTKWDLFGNCYRLLKTGIEHGAMPEQIVVQALQCSHYSILWQLVKITDGSPSKEDLLVLRKTVKSFLAVCQQCLSNVNTPVKEQAFMLLCDLLMIFSHQLMTGGREGLQPLVFNPDTGLQSELLSFVMDHVFIDQDEENQSMEGDEEDEANKIEALHKRRNLLAAFSKLIIYDIVDMHAAADIFKHYMKYYNDYGDIIKETLSKTRQIDKIQCAKTLILSLQQLFNELVQEQGPNLDRTSAHVSGIKELARRFALTFGLDQIKTREAVATLHKDGIEFAFKYQNQKGQEYPPPNLAFLEVLSEFSSKLLRQDKKTVHSYLEKFLTEQMMERREDVWLPLISYRNSLVTGGEDDRMSVNSGSSSSKTSSVRNKKGRPPLHKKRVEDESLDNTWLNRTDTMIQTPGPLPAPQLTSTVLRENSRPMGDQIQEPESEHGSEPDFLHNPQMQISWLGQPKLEDLNRKDRTGMNYMKVRTGVRHAVRGLMEEDAEPIFEDVMMSSRSQLEDMNEEFEDTMVIDLPPSRNRRERAELRPDFFDSAAIIEDDSGFGMPMF

Target ORF information:

RefSeq Version	XM_017005525.1
Organism	Homo sapiens(human)
Definition	Homo sapiens stromal antigen 1 (STAG1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017005525.1

ORF Insert Sequence:

ATGCTATTTA GCAGACATGA CCCACAAGCA GAAGAAGCAT TAGCAAAGAG GAGGGGAAGA 
AACAGCCCGA ATGGAAACCT CATTAGGATG CTGGTTCTTT TCTTTCTTGA AAGTGAGTTA 
CATGAACATG CAGCCTACTT GGTGGACAGT TTATGGGAGA GCTCTCAAGA ACTGTTGAAA 
GACTGGGAAT GTATGACAGA GTTGCTATTA GAAGAACCTG TTCAAGGAGA GGAAGCAATG 
TCTGATCGTC AAGAGAGTGC TCTTATAGAG CTAATGGTTT GTACAATTCG TCAAGCTGCT 
GAGGCACATC CTCCAGTGGG AAGGGGTACC GGCAAGAGAG TGCTAACTGC CAAAGAAAGG 
AAAACTCAAA TTGATGATAG AAACAAATTG ACTGAACATT TTATTATTAC ACTTCCTATG 
TTACTGTCAA AGTATTCTGC AGATGCAGAG AAGGTAGCAA ACTTGCTACA AATCCCACAG 
TATTTTGATT TAGAAATCTA CAGCACAGGT AGAATGGAAA AGCATCTGGA TGCTTTATTA 
AAACAGATTA AGTTTGTTGT GGAGAAACAC GTAGAATCAG ATGTTCTAGA AGCCTGCAGT 
AAAACCTATA GTATCTTATG CAGTGAAGAA TATACCATCC AGAACAGAGT TGACATAGCT 
CGAAGCCAGC TGATTGATGA GTTTGTAGAT CGATTCAATC ATTCTGTGGA AGACCTATTG 
CAAGAGGGAG AAGAAGCTGA TGATGATGAC ATTTACAATG TTCTTTCTAC ATTAAAGCGG 
TTAACTTCTT TTCACAATGC ACATGATCTC ACAAAATGGG ATCTCTTTGG TAATTGCTAC 
AGATTATTGA AGACTGGAAT TGAACATGGA GCCATGCCAG AACAGATAGT CGTGCAAGCA 
CTGCAGTGTT CCCATTATTC GATTCTTTGG CAGTTGGTGA AAATTACTGA TGGCTCTCCT 
TCCAAAGAGG ATTTGTTGGT ATTGAGGAAA ACGGTGAAAT CCTTTTTGGC TGTTTGCCAG 
CAGTGCCTGT CTAATGTTAA TACTCCAGTG AAAGAACAGG CTTTCATGTT ACTCTGTGAT 
CTTCTGATGA TTTTCAGCCA CCAATTAATG ACAGGTGGCA GAGAGGGCCT TCAGCCTTTG 
GTGTTCAATC CAGATACTGG ACTCCAATCT GAACTCCTCA GTTTTGTGAT GGATCACGTT 
TTTATTGACC AAGACGAGGA GAACCAGAGC ATGGAGGGTG ATGAAGAAGA TGAAGCTAAT 
AAAATTGAGG CCTTACATAA AAGAAGGAAT CTACTTGCTG CTTTCAGCAA ACTTATCATT 
TATGACATTG TTGACATGCA TGCAGCTGCA GACATCTTCA AACACTACAT GAAGTATTAC 
AATGACTATG GTGATATTAT TAAGGAAACA CTGAGTAAAA CCAGGCAGAT TGATAAAATT 
CAGTGTGCCA AGACTCTCAT TCTCAGTTTG CAACAGTTAT TTAATGAACT TGTTCAAGAG 
CAAGGTCCCA ACCTAGATAG GACATCTGCC CATGTCAGTG GCATTAAAGA ACTGGCACGT 
CGCTTTGCCC TTACATTTGG ATTGGACCAG ATTAAGACAC GAGAAGCAGT TGCCACACTT 
CACAAGGATG GCATAGAGTT TGCATTTAAA TACCAAAATC AGAAAGGACA AGAGTATCCA 
CCTCCTAATC TGGCTTTTCT TGAAGTACTA AGTGAATTTT CTTCTAAACT TCTTCGACAG 
GACAAAAAGA CAGTTCATTC ATACCTAGAG AAATTCCTTA CCGAGCAGAT GATGGAAAGG 
AGGGAGGATG TATGGCTTCC ACTCATCTCC TATAGAAATT CATTAGTCAC TGGGGGTGAA 
GATGATAGAA TGTCTGTGAA CAGTGGAAGT AGCAGCAGCA AAACCTCATC AGTAAGGAAT 
AAGAAAGGAC GACCTCCACT TCATAAAAAA CGAGTAGAAG ATGAGAGTCT GGATAACACA 
TGGCTAAACA GGACTGACAC CATGATTCAG ACTCCTGGCC CCCTGCCAGC ACCACAACTC 
ACATCCACTG TACTGCGGGA GAACAGTCGG CCCATGGGAG ACCAGATTCA AGAACCTGAG 
TCTGAACATG GTTCTGAACC AGACTTTTTA CACAATCCTC AGATGCAGAT CTCTTGGTTA 
GGCCAGCCGA AGTTAGAAGA CTTAAATCGG AAGGACAGAA CAGGAATGAA CTACATGAAA 
GTGAGAACTG GAGTGAGGCA TGCTGTTCGG GGTCTAATGG AGGAAGATGC TGAGCCCATC 
TTTGAAGATG TGATGATGTC ATCCCGAAGC CAGTTAGAAG ATATGAATGA AGAATTTGAG 
GACACCATGG TTATTGATCT GCCTCCATCA AGAAATCGGC GAGAGAGAGC TGAGCTAAGG 
CCAGACTTCT TTGACTCTGC AGCTATCATA GAAGATGATT CAGGATTTGG AATGCCTATG 
TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

STAG1 ( XM_011512329.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011510631.1 Homo sapiens stromal antigen 1 (STAG1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu63831
Clone ID Related Accession (Same CDS sequence)	XM_011512329.2 , XM_011512331.2 , XM_017005523.1
Accession Version	XM_011512329.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3366bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	cohesin subunit SA-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011512329.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTCGAA ATATGCAGAA TGCAGAAATC ATCAGAAAAA TGACTGAAGA ATTTGATGAG 
GACAGTGGTG ATTATCCTCT TACCATGCCT GGACCTCAGT GGAAAAAATT TCGTTCAAAC 
TTTTGTGAAT TTATTGGAGT CCTGATTCGA CAGTGTCAGT ATAGCATAAT TTATGATGAG 
TATATGATGG ACACAGTAAT CTCCCTTTTG ACGGGTTTGT CAGACTCCCA GGTCAGAGCT 
TTTAGGCATA CAAGTACCCT GGCTGCCATG AAGCTCATGA CTGCTCTGGT GAATGTTGCC 
TTAAACCTCA GTATTCATCA GGATAATACC CAGAGACAAT ATGAAGCCGA GAGAAATAAA 
ATGATTGGGA AGAGAGCCAA TGAAAGGTTG GAGTTACTAC TTCAGAAACG CAAAGAGCTG 
CAAGAAAATC AGGATGAAAT CGAAAATATG ATGAACTCTA TTTTTAAGGG TATATTTGTT 
CATAGATACC GTGATGCTAT TGCTGAGATT AGAGCCATTT GTATTGAAGA AATTGGAGTA 
TGGATGAAAA TGTATAGTGA TGCCTTCCTA AATGACAGTT ACCTAAAATA TGTTGGCTGG 
ACTCTTCATG ACAGGCAAGG GGAAGTCAGG CTGAAGTGTT TGAAAGCTCT GCAGAGTCTA 
TATACCAATA GAGAATTATT CCCCAAATTG GAACTATTCA CTAACCGATT CAAGGATCGC 
ATTGTATCAA TGACACTTGA TAAAGAATAT GATGTTGCTG TGGAAGCTAT TCGATTGGTT 
ACTCTGATAC TTCATGGAAG TGAAGAAGCT CTTTCCAATG AAGACTGTGA AAATGTTTAC 
CACTTGGTGT ACTCGGCACA TCGCCCTGTT GCTGTGGCAG CTGGAGAGTT CCTTCACAAA 
AAGCTATTTA GCAGACATGA CCCACAAGCA GAAGAAGCAT TAGCAAAGAG GAGGGGAAGA 
AACAGCCCGA ATGGAAACCT CATTAGGATG CTGGTTCTTT TCTTTCTTGA AAGTGAGTTA 
CATGAACATG CAGCCTACTT GGTGGACAGT TTATGGGAGA GCTCTCAAGA ACTGTTGAAA 
GACTGGGAAT GTATGACAGA GTTGCTATTA GAAGAACCTG TTCAAGGAGA GGAAGCAATG 
TCTGATCGTC AAGAGAGTGC TCTTATAGAG CTAATGGTTT GTACAATTCG TCAAGCTGCT 
GAGGCACATC CTCCAGTGGG AAGGGGTACC GGCAAGAGAG TGCTAACTGC CAAAGAAAGG 
AAAACTCAAA TTGATGATAG AAACAAATTG ACTGAACATT TTATTATTAC ACTTCCTATG 
TTACTGTCAA AGTATTCTGC AGATGCAGAG AAGGTAGCAA ACTTGCTACA AATCCCACAG 
TATTTTGATT TAGAAATCTA CAGCACAGGT AGAATGGAAA AGCATCTGGA TGCTTTATTA 
AAACAGATTA AGTTTGTTGT GGAGAAACAC GTAGAATCAG ATGTTCTAGA AGCCTGCAGT 
AAAACCTATA GTATCTTATG CAGTGAAGAA TATACCATCC AGAACAGAGT TGACATAGCT 
CGAAGCCAGC TGATTGATGA GTTTGTAGAT CGATTCAATC ATTCTGTGGA AGACCTATTG 
CAAGAGGGAG AAGAAGCTGA TGATGATGAC ATTTACAATG TTCTTTCTAC ATTAAAGCGG 
TTAACTTCTT TTCACAATGC ACATGATCTC ACAAAATGGG ATCTCTTTGG TAATTGCTAC 
AGATTATTGA AGACTGGAAT TGAACATGGA GCCATGCCAG AACAGATAGT CGTGCAAGCA 
CTGCAGTGTT CCCATTATTC GATTCTTTGG CAGTTGGTGA AAATTACTGA TGGCTCTCCT 
TCCAAAGAGG ATTTGTTGGT ATTGAGGAAA ACGGTGAAAT CCTTTTTGGC TGTTTGCCAG 
CAGTGCCTGT CTAATGTTAA TACTCCAGTG AAAGAACAGG CTTTCATGTT ACTCTGTGAT 
CTTCTGATGA TTTTCAGCCA CCAATTAATG ACAGGTGGCA GAGAGGGCCT TCAGCCTTTG 
GTGTTCAATC CAGATACTGG ACTCCAATCT GAACTCCTCA GTTTTGTGAT GGATCACGTT 
TTTATTGACC AAGACGAGGA GAACCAGAGC ATGGAGGGTG ATGAAGAAGA TGAAGCTAAT 
AAAATTGAGG CCTTACATAA AAGAAGGAAT CTACTTGCTG CTTTCAGCAA ACTTATCATT 
TATGACATTG TTGACATGCA TGCAGCTGCA GACATCTTCA AACACTACAT GAAGTATTAC 
AATGACTATG GTGATATTAT TAAGGAAACA CTGAGTAAAA CCAGGCAGAT TGATAAAATT 
CAGTGTGCCA AGACTCTCAT TCTCAGTTTG CAACAGTTAT TTAATGAACT TGTTCAAGAG 
CAAGGTCCCA ACCTAGATAG GACATCTGCC CATGTCAGTG GCATTAAAGA ACTGGCACGT 
CGCTTTGCCC TTACATTTGG ATTGGACCAG ATTAAGACAC GAGAAGCAGT TGCCACACTT 
CACAAGGATG GCATAGAGTT TGCATTTAAA TACCAAAATC AGAAAGGACA AGAGTATCCA 
CCTCCTAATC TGGCTTTTCT TGAAGTACTA AGTGAATTTT CTTCTAAACT TCTTCGACAG 
GACAAAAAGA CAGTTCATTC ATACCTAGAG AAATTCCTTA CCGAGCAGAT GATGGAAAGG 
AGGGAGGATG TATGGCTTCC ACTCATCTCC TATAGAAATT CATTAGTCAC TGGGGGTGAA 
GATGATAGAA TGTCTGTGAA CAGTGGAAGT AGCAGCAGCA AAACCTCATC AGTAAGGAAT 
AAGAAAGGAC GACCTCCACT TCATAAAAAA CGAGTAGAAG ATGAGAGTCT GGATAACACA 
TGGCTAAACA GGACTGACAC CATGATTCAG ACTCCTGGCC CCCTGCCAGC ACCACAACTC 
ACATCCACTG TACTGCGGGA GAACAGTCGG CCCATGGGAG ACCAGATTCA AGAACCTGAG 
TCTGAACATG GTTCTGAACC AGACTTTTTA CACAATCCTC AGATGCAGAT CTCTTGGTTA 
GGCCAGCCGA AGTTAGAAGA CTTAAATCGG AAGGACAGAA CAGGAATGAA CTACATGAAA 
GTGAGAACTG GAGTGAGGCA TGCTGTTCGG GGTCTAATGG AGGAAGATGC TGAGCCCATC 
TTTGAAGATG TGATGATGTC ATCCCGAAGC CAGTTAGAAG ATATGAATGA AGAATTTGAG 
GACACCATGG TTATTGATCT GCCTCCATCA AGAAATCGGC GAGAGAGAGC TGAGCTAAGG 
CCAGACTTCT TTGACTCTGC AGCTATCATA GAAGATGATT CAGGATTTGG AATGCCTATG 
TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011510631.1
CDS	908..4273
Translation	MFRNMQNAEIIRKMTEEFDEDSGDYPLTMPGPQWKKFRSNFCEFIGVLIRQCQYSIIYDEYMMDTVISLLTGLSDSQVRAFRHTSTLAAMKLMTALVNVALNLSIHQDNTQRQYEAERNKMIGKRANERLELLLQKRKELQENQDEIENMMNSIFKGIFVHRYRDAIAEIRAICIEEIGVWMKMYSDAFLNDSYLKYVGWTLHDRQGEVRLKCLKALQSLYTNRELFPKLELFTNRFKDRIVSMTLDKEYDVAVEAIRLVTLILHGSEEALSNEDCENVYHLVYSAHRPVAVAAGEFLHKKLFSRHDPQAEEALAKRRGRNSPNGNLIRMLVLFFLESELHEHAAYLVDSLWESSQELLKDWECMTELLLEEPVQGEEAMSDRQESALIELMVCTIRQAAEAHPPVGRGTGKRVLTAKERKTQIDDRNKLTEHFIITLPMLLSKYSADAEKVANLLQIPQYFDLEIYSTGRMEKHLDALLKQIKFVVEKHVESDVLEACSKTYSILCSEEYTIQNRVDIARSQLIDEFVDRFNHSVEDLLQEGEEADDDDIYNVLSTLKRLTSFHNAHDLTKWDLFGNCYRLLKTGIEHGAMPEQIVVQALQCSHYSILWQLVKITDGSPSKEDLLVLRKTVKSFLAVCQQCLSNVNTPVKEQAFMLLCDLLMIFSHQLMTGGREGLQPLVFNPDTGLQSELLSFVMDHVFIDQDEENQSMEGDEEDEANKIEALHKRRNLLAAFSKLIIYDIVDMHAAADIFKHYMKYYNDYGDIIKETLSKTRQIDKIQCAKTLILSLQQLFNELVQEQGPNLDRTSAHVSGIKELARRFALTFGLDQIKTREAVATLHKDGIEFAFKYQNQKGQEYPPPNLAFLEVLSEFSSKLLRQDKKTVHSYLEKFLTEQMMERREDVWLPLISYRNSLVTGGEDDRMSVNSGSSSSKTSSVRNKKGRPPLHKKRVEDESLDNTWLNRTDTMIQTPGPLPAPQLTSTVLRENSRPMGDQIQEPESEHGSEPDFLHNPQMQISWLGQPKLEDLNRKDRTGMNYMKVRTGVRHAVRGLMEEDAEPIFEDVMMSSRSQLEDMNEEFEDTMVIDLPPSRNRRERAELRPDFFDSAAIIEDDSGFGMPMF

Target ORF information:

RefSeq Version	XM_011512329.2
Organism	Homo sapiens(human)
Definition	Homo sapiens stromal antigen 1 (STAG1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011512329.2

ORF Insert Sequence:

ATGTTTCGAA ATATGCAGAA TGCAGAAATC ATCAGAAAAA TGACTGAAGA ATTTGATGAG 
GACAGTGGTG ATTATCCTCT TACCATGCCT GGACCTCAGT GGAAAAAATT TCGTTCAAAC 
TTTTGTGAAT TTATTGGAGT CCTGATTCGA CAGTGTCAGT ATAGCATAAT TTATGATGAG 
TATATGATGG ACACAGTAAT CTCCCTTTTG ACGGGTTTGT CAGACTCCCA GGTCAGAGCT 
TTTAGGCATA CAAGTACCCT GGCTGCCATG AAGCTCATGA CTGCTCTGGT GAATGTTGCC 
TTAAACCTCA GTATTCATCA GGATAATACC CAGAGACAAT ATGAAGCCGA GAGAAATAAA 
ATGATTGGGA AGAGAGCCAA TGAAAGGTTG GAGTTACTAC TTCAGAAACG CAAAGAGCTG 
CAAGAAAATC AGGATGAAAT CGAAAATATG ATGAACTCTA TTTTTAAGGG TATATTTGTT 
CATAGATACC GTGATGCTAT TGCTGAGATT AGAGCCATTT GTATTGAAGA AATTGGAGTA 
TGGATGAAAA TGTATAGTGA TGCCTTCCTA AATGACAGTT ACCTAAAATA TGTTGGCTGG 
ACTCTTCATG ACAGGCAAGG GGAAGTCAGG CTGAAGTGTT TGAAAGCTCT GCAGAGTCTA 
TATACCAATA GAGAATTATT CCCCAAATTG GAACTATTCA CTAACCGATT CAAGGATCGC 
ATTGTATCAA TGACACTTGA TAAAGAATAT GATGTTGCTG TGGAAGCTAT TCGATTGGTT 
ACTCTGATAC TTCATGGAAG TGAAGAAGCT CTTTCCAATG AAGACTGTGA AAATGTTTAC 
CACTTGGTGT ACTCGGCACA TCGCCCTGTT GCTGTGGCAG CTGGAGAGTT CCTTCACAAA 
AAGCTATTTA GCAGACATGA CCCACAAGCA GAAGAAGCAT TAGCAAAGAG GAGGGGAAGA 
AACAGCCCGA ATGGAAACCT CATTAGGATG CTGGTTCTTT TCTTTCTTGA AAGTGAGTTA 
CATGAACATG CAGCCTACTT GGTGGACAGT TTATGGGAGA GCTCTCAAGA ACTGTTGAAA 
GACTGGGAAT GTATGACAGA GTTGCTATTA GAAGAACCTG TTCAAGGAGA GGAAGCAATG 
TCTGATCGTC AAGAGAGTGC TCTTATAGAG CTAATGGTTT GTACAATTCG TCAAGCTGCT 
GAGGCACATC CTCCAGTGGG AAGGGGTACC GGCAAGAGAG TGCTAACTGC CAAAGAAAGG 
AAAACTCAAA TTGATGATAG AAACAAATTG ACTGAACATT TTATTATTAC ACTTCCTATG 
TTACTGTCAA AGTATTCTGC AGATGCAGAG AAGGTAGCAA ACTTGCTACA AATCCCACAG 
TATTTTGATT TAGAAATCTA CAGCACAGGT AGAATGGAAA AGCATCTGGA TGCTTTATTA 
AAACAGATTA AGTTTGTTGT GGAGAAACAC GTAGAATCAG ATGTTCTAGA AGCCTGCAGT 
AAAACCTATA GTATCTTATG CAGTGAAGAA TATACCATCC AGAACAGAGT TGACATAGCT 
CGAAGCCAGC TGATTGATGA GTTTGTAGAT CGATTCAATC ATTCTGTGGA AGACCTATTG 
CAAGAGGGAG AAGAAGCTGA TGATGATGAC ATTTACAATG TTCTTTCTAC ATTAAAGCGG 
TTAACTTCTT TTCACAATGC ACATGATCTC ACAAAATGGG ATCTCTTTGG TAATTGCTAC 
AGATTATTGA AGACTGGAAT TGAACATGGA GCCATGCCAG AACAGATAGT CGTGCAAGCA 
CTGCAGTGTT CCCATTATTC GATTCTTTGG CAGTTGGTGA AAATTACTGA TGGCTCTCCT 
TCCAAAGAGG ATTTGTTGGT ATTGAGGAAA ACGGTGAAAT CCTTTTTGGC TGTTTGCCAG 
CAGTGCCTGT CTAATGTTAA TACTCCAGTG AAAGAACAGG CTTTCATGTT ACTCTGTGAT 
CTTCTGATGA TTTTCAGCCA CCAATTAATG ACAGGTGGCA GAGAGGGCCT TCAGCCTTTG 
GTGTTCAATC CAGATACTGG ACTCCAATCT GAACTCCTCA GTTTTGTGAT GGATCACGTT 
TTTATTGACC AAGACGAGGA GAACCAGAGC ATGGAGGGTG ATGAAGAAGA TGAAGCTAAT 
AAAATTGAGG CCTTACATAA AAGAAGGAAT CTACTTGCTG CTTTCAGCAA ACTTATCATT 
TATGACATTG TTGACATGCA TGCAGCTGCA GACATCTTCA AACACTACAT GAAGTATTAC 
AATGACTATG GTGATATTAT TAAGGAAACA CTGAGTAAAA CCAGGCAGAT TGATAAAATT 
CAGTGTGCCA AGACTCTCAT TCTCAGTTTG CAACAGTTAT TTAATGAACT TGTTCAAGAG 
CAAGGTCCCA ACCTAGATAG GACATCTGCC CATGTCAGTG GCATTAAAGA ACTGGCACGT 
CGCTTTGCCC TTACATTTGG ATTGGACCAG ATTAAGACAC GAGAAGCAGT TGCCACACTT 
CACAAGGATG GCATAGAGTT TGCATTTAAA TACCAAAATC AGAAAGGACA AGAGTATCCA 
CCTCCTAATC TGGCTTTTCT TGAAGTACTA AGTGAATTTT CTTCTAAACT TCTTCGACAG 
GACAAAAAGA CAGTTCATTC ATACCTAGAG AAATTCCTTA CCGAGCAGAT GATGGAAAGG 
AGGGAGGATG TATGGCTTCC ACTCATCTCC TATAGAAATT CATTAGTCAC TGGGGGTGAA 
GATGATAGAA TGTCTGTGAA CAGTGGAAGT AGCAGCAGCA AAACCTCATC AGTAAGGAAT 
AAGAAAGGAC GACCTCCACT TCATAAAAAA CGAGTAGAAG ATGAGAGTCT GGATAACACA 
TGGCTAAACA GGACTGACAC CATGATTCAG ACTCCTGGCC CCCTGCCAGC ACCACAACTC 
ACATCCACTG TACTGCGGGA GAACAGTCGG CCCATGGGAG ACCAGATTCA AGAACCTGAG 
TCTGAACATG GTTCTGAACC AGACTTTTTA CACAATCCTC AGATGCAGAT CTCTTGGTTA 
GGCCAGCCGA AGTTAGAAGA CTTAAATCGG AAGGACAGAA CAGGAATGAA CTACATGAAA 
GTGAGAACTG GAGTGAGGCA TGCTGTTCGG GGTCTAATGG AGGAAGATGC TGAGCCCATC 
TTTGAAGATG TGATGATGTC ATCCCGAAGC CAGTTAGAAG ATATGAATGA AGAATTTGAG 
GACACCATGG TTATTGATCT GCCTCCATCA AGAAATCGGC GAGAGAGAGC TGAGCTAAGG 
CCAGACTTCT TTGACTCTGC AGCTATCATA GAAGATGATT CAGGATTTGG AATGCCTATG 
TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

STAG1 ( XM_011512331.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011510633.1 Homo sapiens stromal antigen 1 (STAG1), transcript variant X2, mRNA.

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CloneID	OHu63831
Clone ID Related Accession (Same CDS sequence)	XM_011512329.2 , XM_011512331.2 , XM_017005523.1
Accession Version	XM_011512331.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3366bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	cohesin subunit SA-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011512331.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTCGAA ATATGCAGAA TGCAGAAATC ATCAGAAAAA TGACTGAAGA ATTTGATGAG 
GACAGTGGTG ATTATCCTCT TACCATGCCT GGACCTCAGT GGAAAAAATT TCGTTCAAAC 
TTTTGTGAAT TTATTGGAGT CCTGATTCGA CAGTGTCAGT ATAGCATAAT TTATGATGAG 
TATATGATGG ACACAGTAAT CTCCCTTTTG ACGGGTTTGT CAGACTCCCA GGTCAGAGCT 
TTTAGGCATA CAAGTACCCT GGCTGCCATG AAGCTCATGA CTGCTCTGGT GAATGTTGCC 
TTAAACCTCA GTATTCATCA GGATAATACC CAGAGACAAT ATGAAGCCGA GAGAAATAAA 
ATGATTGGGA AGAGAGCCAA TGAAAGGTTG GAGTTACTAC TTCAGAAACG CAAAGAGCTG 
CAAGAAAATC AGGATGAAAT CGAAAATATG ATGAACTCTA TTTTTAAGGG TATATTTGTT 
CATAGATACC GTGATGCTAT TGCTGAGATT AGAGCCATTT GTATTGAAGA AATTGGAGTA 
TGGATGAAAA TGTATAGTGA TGCCTTCCTA AATGACAGTT ACCTAAAATA TGTTGGCTGG 
ACTCTTCATG ACAGGCAAGG GGAAGTCAGG CTGAAGTGTT TGAAAGCTCT GCAGAGTCTA 
TATACCAATA GAGAATTATT CCCCAAATTG GAACTATTCA CTAACCGATT CAAGGATCGC 
ATTGTATCAA TGACACTTGA TAAAGAATAT GATGTTGCTG TGGAAGCTAT TCGATTGGTT 
ACTCTGATAC TTCATGGAAG TGAAGAAGCT CTTTCCAATG AAGACTGTGA AAATGTTTAC 
CACTTGGTGT ACTCGGCACA TCGCCCTGTT GCTGTGGCAG CTGGAGAGTT CCTTCACAAA 
AAGCTATTTA GCAGACATGA CCCACAAGCA GAAGAAGCAT TAGCAAAGAG GAGGGGAAGA 
AACAGCCCGA ATGGAAACCT CATTAGGATG CTGGTTCTTT TCTTTCTTGA AAGTGAGTTA 
CATGAACATG CAGCCTACTT GGTGGACAGT TTATGGGAGA GCTCTCAAGA ACTGTTGAAA 
GACTGGGAAT GTATGACAGA GTTGCTATTA GAAGAACCTG TTCAAGGAGA GGAAGCAATG 
TCTGATCGTC AAGAGAGTGC TCTTATAGAG CTAATGGTTT GTACAATTCG TCAAGCTGCT 
GAGGCACATC CTCCAGTGGG AAGGGGTACC GGCAAGAGAG TGCTAACTGC CAAAGAAAGG 
AAAACTCAAA TTGATGATAG AAACAAATTG ACTGAACATT TTATTATTAC ACTTCCTATG 
TTACTGTCAA AGTATTCTGC AGATGCAGAG AAGGTAGCAA ACTTGCTACA AATCCCACAG 
TATTTTGATT TAGAAATCTA CAGCACAGGT AGAATGGAAA AGCATCTGGA TGCTTTATTA 
AAACAGATTA AGTTTGTTGT GGAGAAACAC GTAGAATCAG ATGTTCTAGA AGCCTGCAGT 
AAAACCTATA GTATCTTATG CAGTGAAGAA TATACCATCC AGAACAGAGT TGACATAGCT 
CGAAGCCAGC TGATTGATGA GTTTGTAGAT CGATTCAATC ATTCTGTGGA AGACCTATTG 
CAAGAGGGAG AAGAAGCTGA TGATGATGAC ATTTACAATG TTCTTTCTAC ATTAAAGCGG 
TTAACTTCTT TTCACAATGC ACATGATCTC ACAAAATGGG ATCTCTTTGG TAATTGCTAC 
AGATTATTGA AGACTGGAAT TGAACATGGA GCCATGCCAG AACAGATAGT CGTGCAAGCA 
CTGCAGTGTT CCCATTATTC GATTCTTTGG CAGTTGGTGA AAATTACTGA TGGCTCTCCT 
TCCAAAGAGG ATTTGTTGGT ATTGAGGAAA ACGGTGAAAT CCTTTTTGGC TGTTTGCCAG 
CAGTGCCTGT CTAATGTTAA TACTCCAGTG AAAGAACAGG CTTTCATGTT ACTCTGTGAT 
CTTCTGATGA TTTTCAGCCA CCAATTAATG ACAGGTGGCA GAGAGGGCCT TCAGCCTTTG 
GTGTTCAATC CAGATACTGG ACTCCAATCT GAACTCCTCA GTTTTGTGAT GGATCACGTT 
TTTATTGACC AAGACGAGGA GAACCAGAGC ATGGAGGGTG ATGAAGAAGA TGAAGCTAAT 
AAAATTGAGG CCTTACATAA AAGAAGGAAT CTACTTGCTG CTTTCAGCAA ACTTATCATT 
TATGACATTG TTGACATGCA TGCAGCTGCA GACATCTTCA AACACTACAT GAAGTATTAC 
AATGACTATG GTGATATTAT TAAGGAAACA CTGAGTAAAA CCAGGCAGAT TGATAAAATT 
CAGTGTGCCA AGACTCTCAT TCTCAGTTTG CAACAGTTAT TTAATGAACT TGTTCAAGAG 
CAAGGTCCCA ACCTAGATAG GACATCTGCC CATGTCAGTG GCATTAAAGA ACTGGCACGT 
CGCTTTGCCC TTACATTTGG ATTGGACCAG ATTAAGACAC GAGAAGCAGT TGCCACACTT 
CACAAGGATG GCATAGAGTT TGCATTTAAA TACCAAAATC AGAAAGGACA AGAGTATCCA 
CCTCCTAATC TGGCTTTTCT TGAAGTACTA AGTGAATTTT CTTCTAAACT TCTTCGACAG 
GACAAAAAGA CAGTTCATTC ATACCTAGAG AAATTCCTTA CCGAGCAGAT GATGGAAAGG 
AGGGAGGATG TATGGCTTCC ACTCATCTCC TATAGAAATT CATTAGTCAC TGGGGGTGAA 
GATGATAGAA TGTCTGTGAA CAGTGGAAGT AGCAGCAGCA AAACCTCATC AGTAAGGAAT 
AAGAAAGGAC GACCTCCACT TCATAAAAAA CGAGTAGAAG ATGAGAGTCT GGATAACACA 
TGGCTAAACA GGACTGACAC CATGATTCAG ACTCCTGGCC CCCTGCCAGC ACCACAACTC 
ACATCCACTG TACTGCGGGA GAACAGTCGG CCCATGGGAG ACCAGATTCA AGAACCTGAG 
TCTGAACATG GTTCTGAACC AGACTTTTTA CACAATCCTC AGATGCAGAT CTCTTGGTTA 
GGCCAGCCGA AGTTAGAAGA CTTAAATCGG AAGGACAGAA CAGGAATGAA CTACATGAAA 
GTGAGAACTG GAGTGAGGCA TGCTGTTCGG GGTCTAATGG AGGAAGATGC TGAGCCCATC 
TTTGAAGATG TGATGATGTC ATCCCGAAGC CAGTTAGAAG ATATGAATGA AGAATTTGAG 
GACACCATGG TTATTGATCT GCCTCCATCA AGAAATCGGC GAGAGAGAGC TGAGCTAAGG 
CCAGACTTCT TTGACTCTGC AGCTATCATA GAAGATGATT CAGGATTTGG AATGCCTATG 
TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011510633.1
CDS	838..4203
Translation	MFRNMQNAEIIRKMTEEFDEDSGDYPLTMPGPQWKKFRSNFCEFIGVLIRQCQYSIIYDEYMMDTVISLLTGLSDSQVRAFRHTSTLAAMKLMTALVNVALNLSIHQDNTQRQYEAERNKMIGKRANERLELLLQKRKELQENQDEIENMMNSIFKGIFVHRYRDAIAEIRAICIEEIGVWMKMYSDAFLNDSYLKYVGWTLHDRQGEVRLKCLKALQSLYTNRELFPKLELFTNRFKDRIVSMTLDKEYDVAVEAIRLVTLILHGSEEALSNEDCENVYHLVYSAHRPVAVAAGEFLHKKLFSRHDPQAEEALAKRRGRNSPNGNLIRMLVLFFLESELHEHAAYLVDSLWESSQELLKDWECMTELLLEEPVQGEEAMSDRQESALIELMVCTIRQAAEAHPPVGRGTGKRVLTAKERKTQIDDRNKLTEHFIITLPMLLSKYSADAEKVANLLQIPQYFDLEIYSTGRMEKHLDALLKQIKFVVEKHVESDVLEACSKTYSILCSEEYTIQNRVDIARSQLIDEFVDRFNHSVEDLLQEGEEADDDDIYNVLSTLKRLTSFHNAHDLTKWDLFGNCYRLLKTGIEHGAMPEQIVVQALQCSHYSILWQLVKITDGSPSKEDLLVLRKTVKSFLAVCQQCLSNVNTPVKEQAFMLLCDLLMIFSHQLMTGGREGLQPLVFNPDTGLQSELLSFVMDHVFIDQDEENQSMEGDEEDEANKIEALHKRRNLLAAFSKLIIYDIVDMHAAADIFKHYMKYYNDYGDIIKETLSKTRQIDKIQCAKTLILSLQQLFNELVQEQGPNLDRTSAHVSGIKELARRFALTFGLDQIKTREAVATLHKDGIEFAFKYQNQKGQEYPPPNLAFLEVLSEFSSKLLRQDKKTVHSYLEKFLTEQMMERREDVWLPLISYRNSLVTGGEDDRMSVNSGSSSSKTSSVRNKKGRPPLHKKRVEDESLDNTWLNRTDTMIQTPGPLPAPQLTSTVLRENSRPMGDQIQEPESEHGSEPDFLHNPQMQISWLGQPKLEDLNRKDRTGMNYMKVRTGVRHAVRGLMEEDAEPIFEDVMMSSRSQLEDMNEEFEDTMVIDLPPSRNRRERAELRPDFFDSAAIIEDDSGFGMPMF

Target ORF information:

RefSeq Version	XM_011512331.2
Organism	Homo sapiens(human)
Definition	Homo sapiens stromal antigen 1 (STAG1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011512331.2

ORF Insert Sequence:

ATGTTTCGAA ATATGCAGAA TGCAGAAATC ATCAGAAAAA TGACTGAAGA ATTTGATGAG 
GACAGTGGTG ATTATCCTCT TACCATGCCT GGACCTCAGT GGAAAAAATT TCGTTCAAAC 
TTTTGTGAAT TTATTGGAGT CCTGATTCGA CAGTGTCAGT ATAGCATAAT TTATGATGAG 
TATATGATGG ACACAGTAAT CTCCCTTTTG ACGGGTTTGT CAGACTCCCA GGTCAGAGCT 
TTTAGGCATA CAAGTACCCT GGCTGCCATG AAGCTCATGA CTGCTCTGGT GAATGTTGCC 
TTAAACCTCA GTATTCATCA GGATAATACC CAGAGACAAT ATGAAGCCGA GAGAAATAAA 
ATGATTGGGA AGAGAGCCAA TGAAAGGTTG GAGTTACTAC TTCAGAAACG CAAAGAGCTG 
CAAGAAAATC AGGATGAAAT CGAAAATATG ATGAACTCTA TTTTTAAGGG TATATTTGTT 
CATAGATACC GTGATGCTAT TGCTGAGATT AGAGCCATTT GTATTGAAGA AATTGGAGTA 
TGGATGAAAA TGTATAGTGA TGCCTTCCTA AATGACAGTT ACCTAAAATA TGTTGGCTGG 
ACTCTTCATG ACAGGCAAGG GGAAGTCAGG CTGAAGTGTT TGAAAGCTCT GCAGAGTCTA 
TATACCAATA GAGAATTATT CCCCAAATTG GAACTATTCA CTAACCGATT CAAGGATCGC 
ATTGTATCAA TGACACTTGA TAAAGAATAT GATGTTGCTG TGGAAGCTAT TCGATTGGTT 
ACTCTGATAC TTCATGGAAG TGAAGAAGCT CTTTCCAATG AAGACTGTGA AAATGTTTAC 
CACTTGGTGT ACTCGGCACA TCGCCCTGTT GCTGTGGCAG CTGGAGAGTT CCTTCACAAA 
AAGCTATTTA GCAGACATGA CCCACAAGCA GAAGAAGCAT TAGCAAAGAG GAGGGGAAGA 
AACAGCCCGA ATGGAAACCT CATTAGGATG CTGGTTCTTT TCTTTCTTGA AAGTGAGTTA 
CATGAACATG CAGCCTACTT GGTGGACAGT TTATGGGAGA GCTCTCAAGA ACTGTTGAAA 
GACTGGGAAT GTATGACAGA GTTGCTATTA GAAGAACCTG TTCAAGGAGA GGAAGCAATG 
TCTGATCGTC AAGAGAGTGC TCTTATAGAG CTAATGGTTT GTACAATTCG TCAAGCTGCT 
GAGGCACATC CTCCAGTGGG AAGGGGTACC GGCAAGAGAG TGCTAACTGC CAAAGAAAGG 
AAAACTCAAA TTGATGATAG AAACAAATTG ACTGAACATT TTATTATTAC ACTTCCTATG 
TTACTGTCAA AGTATTCTGC AGATGCAGAG AAGGTAGCAA ACTTGCTACA AATCCCACAG 
TATTTTGATT TAGAAATCTA CAGCACAGGT AGAATGGAAA AGCATCTGGA TGCTTTATTA 
AAACAGATTA AGTTTGTTGT GGAGAAACAC GTAGAATCAG ATGTTCTAGA AGCCTGCAGT 
AAAACCTATA GTATCTTATG CAGTGAAGAA TATACCATCC AGAACAGAGT TGACATAGCT 
CGAAGCCAGC TGATTGATGA GTTTGTAGAT CGATTCAATC ATTCTGTGGA AGACCTATTG 
CAAGAGGGAG AAGAAGCTGA TGATGATGAC ATTTACAATG TTCTTTCTAC ATTAAAGCGG 
TTAACTTCTT TTCACAATGC ACATGATCTC ACAAAATGGG ATCTCTTTGG TAATTGCTAC 
AGATTATTGA AGACTGGAAT TGAACATGGA GCCATGCCAG AACAGATAGT CGTGCAAGCA 
CTGCAGTGTT CCCATTATTC GATTCTTTGG CAGTTGGTGA AAATTACTGA TGGCTCTCCT 
TCCAAAGAGG ATTTGTTGGT ATTGAGGAAA ACGGTGAAAT CCTTTTTGGC TGTTTGCCAG 
CAGTGCCTGT CTAATGTTAA TACTCCAGTG AAAGAACAGG CTTTCATGTT ACTCTGTGAT 
CTTCTGATGA TTTTCAGCCA CCAATTAATG ACAGGTGGCA GAGAGGGCCT TCAGCCTTTG 
GTGTTCAATC CAGATACTGG ACTCCAATCT GAACTCCTCA GTTTTGTGAT GGATCACGTT 
TTTATTGACC AAGACGAGGA GAACCAGAGC ATGGAGGGTG ATGAAGAAGA TGAAGCTAAT 
AAAATTGAGG CCTTACATAA AAGAAGGAAT CTACTTGCTG CTTTCAGCAA ACTTATCATT 
TATGACATTG TTGACATGCA TGCAGCTGCA GACATCTTCA AACACTACAT GAAGTATTAC 
AATGACTATG GTGATATTAT TAAGGAAACA CTGAGTAAAA CCAGGCAGAT TGATAAAATT 
CAGTGTGCCA AGACTCTCAT TCTCAGTTTG CAACAGTTAT TTAATGAACT TGTTCAAGAG 
CAAGGTCCCA ACCTAGATAG GACATCTGCC CATGTCAGTG GCATTAAAGA ACTGGCACGT 
CGCTTTGCCC TTACATTTGG ATTGGACCAG ATTAAGACAC GAGAAGCAGT TGCCACACTT 
CACAAGGATG GCATAGAGTT TGCATTTAAA TACCAAAATC AGAAAGGACA AGAGTATCCA 
CCTCCTAATC TGGCTTTTCT TGAAGTACTA AGTGAATTTT CTTCTAAACT TCTTCGACAG 
GACAAAAAGA CAGTTCATTC ATACCTAGAG AAATTCCTTA CCGAGCAGAT GATGGAAAGG 
AGGGAGGATG TATGGCTTCC ACTCATCTCC TATAGAAATT CATTAGTCAC TGGGGGTGAA 
GATGATAGAA TGTCTGTGAA CAGTGGAAGT AGCAGCAGCA AAACCTCATC AGTAAGGAAT 
AAGAAAGGAC GACCTCCACT TCATAAAAAA CGAGTAGAAG ATGAGAGTCT GGATAACACA 
TGGCTAAACA GGACTGACAC CATGATTCAG ACTCCTGGCC CCCTGCCAGC ACCACAACTC 
ACATCCACTG TACTGCGGGA GAACAGTCGG CCCATGGGAG ACCAGATTCA AGAACCTGAG 
TCTGAACATG GTTCTGAACC AGACTTTTTA CACAATCCTC AGATGCAGAT CTCTTGGTTA 
GGCCAGCCGA AGTTAGAAGA CTTAAATCGG AAGGACAGAA CAGGAATGAA CTACATGAAA 
GTGAGAACTG GAGTGAGGCA TGCTGTTCGG GGTCTAATGG AGGAAGATGC TGAGCCCATC 
TTTGAAGATG TGATGATGTC ATCCCGAAGC CAGTTAGAAG ATATGAATGA AGAATTTGAG 
GACACCATGG TTATTGATCT GCCTCCATCA AGAAATCGGC GAGAGAGAGC TGAGCTAAGG 
CCAGACTTCT TTGACTCTGC AGCTATCATA GAAGATGATT CAGGATTTGG AATGCCTATG 
TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

STAG1 ( XM_017005523.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016861012.1 Homo sapiens stromal antigen 1 (STAG1), transcript variant X3, mRNA.

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CloneID	OHu63831
Clone ID Related Accession (Same CDS sequence)	XM_011512329.2 , XM_011512331.2 , XM_017005523.1
Accession Version	XM_017005523.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3366bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	cohesin subunit SA-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTCGAA ATATGCAGAA TGCAGAAATC ATCAGAAAAA TGACTGAAGA ATTTGATGAG 
GACAGTGGTG ATTATCCTCT TACCATGCCT GGACCTCAGT GGAAAAAATT TCGTTCAAAC 
TTTTGTGAAT TTATTGGAGT CCTGATTCGA CAGTGTCAGT ATAGCATAAT TTATGATGAG 
TATATGATGG ACACAGTAAT CTCCCTTTTG ACGGGTTTGT CAGACTCCCA GGTCAGAGCT 
TTTAGGCATA CAAGTACCCT GGCTGCCATG AAGCTCATGA CTGCTCTGGT GAATGTTGCC 
TTAAACCTCA GTATTCATCA GGATAATACC CAGAGACAAT ATGAAGCCGA GAGAAATAAA 
ATGATTGGGA AGAGAGCCAA TGAAAGGTTG GAGTTACTAC TTCAGAAACG CAAAGAGCTG 
CAAGAAAATC AGGATGAAAT CGAAAATATG ATGAACTCTA TTTTTAAGGG TATATTTGTT 
CATAGATACC GTGATGCTAT TGCTGAGATT AGAGCCATTT GTATTGAAGA AATTGGAGTA 
TGGATGAAAA TGTATAGTGA TGCCTTCCTA AATGACAGTT ACCTAAAATA TGTTGGCTGG 
ACTCTTCATG ACAGGCAAGG GGAAGTCAGG CTGAAGTGTT TGAAAGCTCT GCAGAGTCTA 
TATACCAATA GAGAATTATT CCCCAAATTG GAACTATTCA CTAACCGATT CAAGGATCGC 
ATTGTATCAA TGACACTTGA TAAAGAATAT GATGTTGCTG TGGAAGCTAT TCGATTGGTT 
ACTCTGATAC TTCATGGAAG TGAAGAAGCT CTTTCCAATG AAGACTGTGA AAATGTTTAC 
CACTTGGTGT ACTCGGCACA TCGCCCTGTT GCTGTGGCAG CTGGAGAGTT CCTTCACAAA 
AAGCTATTTA GCAGACATGA CCCACAAGCA GAAGAAGCAT TAGCAAAGAG GAGGGGAAGA 
AACAGCCCGA ATGGAAACCT CATTAGGATG CTGGTTCTTT TCTTTCTTGA AAGTGAGTTA 
CATGAACATG CAGCCTACTT GGTGGACAGT TTATGGGAGA GCTCTCAAGA ACTGTTGAAA 
GACTGGGAAT GTATGACAGA GTTGCTATTA GAAGAACCTG TTCAAGGAGA GGAAGCAATG 
TCTGATCGTC AAGAGAGTGC TCTTATAGAG CTAATGGTTT GTACAATTCG TCAAGCTGCT 
GAGGCACATC CTCCAGTGGG AAGGGGTACC GGCAAGAGAG TGCTAACTGC CAAAGAAAGG 
AAAACTCAAA TTGATGATAG AAACAAATTG ACTGAACATT TTATTATTAC ACTTCCTATG 
TTACTGTCAA AGTATTCTGC AGATGCAGAG AAGGTAGCAA ACTTGCTACA AATCCCACAG 
TATTTTGATT TAGAAATCTA CAGCACAGGT AGAATGGAAA AGCATCTGGA TGCTTTATTA 
AAACAGATTA AGTTTGTTGT GGAGAAACAC GTAGAATCAG ATGTTCTAGA AGCCTGCAGT 
AAAACCTATA GTATCTTATG CAGTGAAGAA TATACCATCC AGAACAGAGT TGACATAGCT 
CGAAGCCAGC TGATTGATGA GTTTGTAGAT CGATTCAATC ATTCTGTGGA AGACCTATTG 
CAAGAGGGAG AAGAAGCTGA TGATGATGAC ATTTACAATG TTCTTTCTAC ATTAAAGCGG 
TTAACTTCTT TTCACAATGC ACATGATCTC ACAAAATGGG ATCTCTTTGG TAATTGCTAC 
AGATTATTGA AGACTGGAAT TGAACATGGA GCCATGCCAG AACAGATAGT CGTGCAAGCA 
CTGCAGTGTT CCCATTATTC GATTCTTTGG CAGTTGGTGA AAATTACTGA TGGCTCTCCT 
TCCAAAGAGG ATTTGTTGGT ATTGAGGAAA ACGGTGAAAT CCTTTTTGGC TGTTTGCCAG 
CAGTGCCTGT CTAATGTTAA TACTCCAGTG AAAGAACAGG CTTTCATGTT ACTCTGTGAT 
CTTCTGATGA TTTTCAGCCA CCAATTAATG ACAGGTGGCA GAGAGGGCCT TCAGCCTTTG 
GTGTTCAATC CAGATACTGG ACTCCAATCT GAACTCCTCA GTTTTGTGAT GGATCACGTT 
TTTATTGACC AAGACGAGGA GAACCAGAGC ATGGAGGGTG ATGAAGAAGA TGAAGCTAAT 
AAAATTGAGG CCTTACATAA AAGAAGGAAT CTACTTGCTG CTTTCAGCAA ACTTATCATT 
TATGACATTG TTGACATGCA TGCAGCTGCA GACATCTTCA AACACTACAT GAAGTATTAC 
AATGACTATG GTGATATTAT TAAGGAAACA CTGAGTAAAA CCAGGCAGAT TGATAAAATT 
CAGTGTGCCA AGACTCTCAT TCTCAGTTTG CAACAGTTAT TTAATGAACT TGTTCAAGAG 
CAAGGTCCCA ACCTAGATAG GACATCTGCC CATGTCAGTG GCATTAAAGA ACTGGCACGT 
CGCTTTGCCC TTACATTTGG ATTGGACCAG ATTAAGACAC GAGAAGCAGT TGCCACACTT 
CACAAGGATG GCATAGAGTT TGCATTTAAA TACCAAAATC AGAAAGGACA AGAGTATCCA 
CCTCCTAATC TGGCTTTTCT TGAAGTACTA AGTGAATTTT CTTCTAAACT TCTTCGACAG 
GACAAAAAGA CAGTTCATTC ATACCTAGAG AAATTCCTTA CCGAGCAGAT GATGGAAAGG 
AGGGAGGATG TATGGCTTCC ACTCATCTCC TATAGAAATT CATTAGTCAC TGGGGGTGAA 
GATGATAGAA TGTCTGTGAA CAGTGGAAGT AGCAGCAGCA AAACCTCATC AGTAAGGAAT 
AAGAAAGGAC GACCTCCACT TCATAAAAAA CGAGTAGAAG ATGAGAGTCT GGATAACACA 
TGGCTAAACA GGACTGACAC CATGATTCAG ACTCCTGGCC CCCTGCCAGC ACCACAACTC 
ACATCCACTG TACTGCGGGA GAACAGTCGG CCCATGGGAG ACCAGATTCA AGAACCTGAG 
TCTGAACATG GTTCTGAACC AGACTTTTTA CACAATCCTC AGATGCAGAT CTCTTGGTTA 
GGCCAGCCGA AGTTAGAAGA CTTAAATCGG AAGGACAGAA CAGGAATGAA CTACATGAAA 
GTGAGAACTG GAGTGAGGCA TGCTGTTCGG GGTCTAATGG AGGAAGATGC TGAGCCCATC 
TTTGAAGATG TGATGATGTC ATCCCGAAGC CAGTTAGAAG ATATGAATGA AGAATTTGAG 
GACACCATGG TTATTGATCT GCCTCCATCA AGAAATCGGC GAGAGAGAGC TGAGCTAAGG 
CCAGACTTCT TTGACTCTGC AGCTATCATA GAAGATGATT CAGGATTTGG AATGCCTATG 
TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016861012.1
CDS	582..3947
Translation	MFRNMQNAEIIRKMTEEFDEDSGDYPLTMPGPQWKKFRSNFCEFIGVLIRQCQYSIIYDEYMMDTVISLLTGLSDSQVRAFRHTSTLAAMKLMTALVNVALNLSIHQDNTQRQYEAERNKMIGKRANERLELLLQKRKELQENQDEIENMMNSIFKGIFVHRYRDAIAEIRAICIEEIGVWMKMYSDAFLNDSYLKYVGWTLHDRQGEVRLKCLKALQSLYTNRELFPKLELFTNRFKDRIVSMTLDKEYDVAVEAIRLVTLILHGSEEALSNEDCENVYHLVYSAHRPVAVAAGEFLHKKLFSRHDPQAEEALAKRRGRNSPNGNLIRMLVLFFLESELHEHAAYLVDSLWESSQELLKDWECMTELLLEEPVQGEEAMSDRQESALIELMVCTIRQAAEAHPPVGRGTGKRVLTAKERKTQIDDRNKLTEHFIITLPMLLSKYSADAEKVANLLQIPQYFDLEIYSTGRMEKHLDALLKQIKFVVEKHVESDVLEACSKTYSILCSEEYTIQNRVDIARSQLIDEFVDRFNHSVEDLLQEGEEADDDDIYNVLSTLKRLTSFHNAHDLTKWDLFGNCYRLLKTGIEHGAMPEQIVVQALQCSHYSILWQLVKITDGSPSKEDLLVLRKTVKSFLAVCQQCLSNVNTPVKEQAFMLLCDLLMIFSHQLMTGGREGLQPLVFNPDTGLQSELLSFVMDHVFIDQDEENQSMEGDEEDEANKIEALHKRRNLLAAFSKLIIYDIVDMHAAADIFKHYMKYYNDYGDIIKETLSKTRQIDKIQCAKTLILSLQQLFNELVQEQGPNLDRTSAHVSGIKELARRFALTFGLDQIKTREAVATLHKDGIEFAFKYQNQKGQEYPPPNLAFLEVLSEFSSKLLRQDKKTVHSYLEKFLTEQMMERREDVWLPLISYRNSLVTGGEDDRMSVNSGSSSSKTSSVRNKKGRPPLHKKRVEDESLDNTWLNRTDTMIQTPGPLPAPQLTSTVLRENSRPMGDQIQEPESEHGSEPDFLHNPQMQISWLGQPKLEDLNRKDRTGMNYMKVRTGVRHAVRGLMEEDAEPIFEDVMMSSRSQLEDMNEEFEDTMVIDLPPSRNRRERAELRPDFFDSAAIIEDDSGFGMPMF

Target ORF information:

RefSeq Version	XM_017005523.1
Organism	Homo sapiens(human)
Definition	Homo sapiens stromal antigen 1 (STAG1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017005523.1

ORF Insert Sequence:

ATGTTTCGAA ATATGCAGAA TGCAGAAATC ATCAGAAAAA TGACTGAAGA ATTTGATGAG 
GACAGTGGTG ATTATCCTCT TACCATGCCT GGACCTCAGT GGAAAAAATT TCGTTCAAAC 
TTTTGTGAAT TTATTGGAGT CCTGATTCGA CAGTGTCAGT ATAGCATAAT TTATGATGAG 
TATATGATGG ACACAGTAAT CTCCCTTTTG ACGGGTTTGT CAGACTCCCA GGTCAGAGCT 
TTTAGGCATA CAAGTACCCT GGCTGCCATG AAGCTCATGA CTGCTCTGGT GAATGTTGCC 
TTAAACCTCA GTATTCATCA GGATAATACC CAGAGACAAT ATGAAGCCGA GAGAAATAAA 
ATGATTGGGA AGAGAGCCAA TGAAAGGTTG GAGTTACTAC TTCAGAAACG CAAAGAGCTG 
CAAGAAAATC AGGATGAAAT CGAAAATATG ATGAACTCTA TTTTTAAGGG TATATTTGTT 
CATAGATACC GTGATGCTAT TGCTGAGATT AGAGCCATTT GTATTGAAGA AATTGGAGTA 
TGGATGAAAA TGTATAGTGA TGCCTTCCTA AATGACAGTT ACCTAAAATA TGTTGGCTGG 
ACTCTTCATG ACAGGCAAGG GGAAGTCAGG CTGAAGTGTT TGAAAGCTCT GCAGAGTCTA 
TATACCAATA GAGAATTATT CCCCAAATTG GAACTATTCA CTAACCGATT CAAGGATCGC 
ATTGTATCAA TGACACTTGA TAAAGAATAT GATGTTGCTG TGGAAGCTAT TCGATTGGTT 
ACTCTGATAC TTCATGGAAG TGAAGAAGCT CTTTCCAATG AAGACTGTGA AAATGTTTAC 
CACTTGGTGT ACTCGGCACA TCGCCCTGTT GCTGTGGCAG CTGGAGAGTT CCTTCACAAA 
AAGCTATTTA GCAGACATGA CCCACAAGCA GAAGAAGCAT TAGCAAAGAG GAGGGGAAGA 
AACAGCCCGA ATGGAAACCT CATTAGGATG CTGGTTCTTT TCTTTCTTGA AAGTGAGTTA 
CATGAACATG CAGCCTACTT GGTGGACAGT TTATGGGAGA GCTCTCAAGA ACTGTTGAAA 
GACTGGGAAT GTATGACAGA GTTGCTATTA GAAGAACCTG TTCAAGGAGA GGAAGCAATG 
TCTGATCGTC AAGAGAGTGC TCTTATAGAG CTAATGGTTT GTACAATTCG TCAAGCTGCT 
GAGGCACATC CTCCAGTGGG AAGGGGTACC GGCAAGAGAG TGCTAACTGC CAAAGAAAGG 
AAAACTCAAA TTGATGATAG AAACAAATTG ACTGAACATT TTATTATTAC ACTTCCTATG 
TTACTGTCAA AGTATTCTGC AGATGCAGAG AAGGTAGCAA ACTTGCTACA AATCCCACAG 
TATTTTGATT TAGAAATCTA CAGCACAGGT AGAATGGAAA AGCATCTGGA TGCTTTATTA 
AAACAGATTA AGTTTGTTGT GGAGAAACAC GTAGAATCAG ATGTTCTAGA AGCCTGCAGT 
AAAACCTATA GTATCTTATG CAGTGAAGAA TATACCATCC AGAACAGAGT TGACATAGCT 
CGAAGCCAGC TGATTGATGA GTTTGTAGAT CGATTCAATC ATTCTGTGGA AGACCTATTG 
CAAGAGGGAG AAGAAGCTGA TGATGATGAC ATTTACAATG TTCTTTCTAC ATTAAAGCGG 
TTAACTTCTT TTCACAATGC ACATGATCTC ACAAAATGGG ATCTCTTTGG TAATTGCTAC 
AGATTATTGA AGACTGGAAT TGAACATGGA GCCATGCCAG AACAGATAGT CGTGCAAGCA 
CTGCAGTGTT CCCATTATTC GATTCTTTGG CAGTTGGTGA AAATTACTGA TGGCTCTCCT 
TCCAAAGAGG ATTTGTTGGT ATTGAGGAAA ACGGTGAAAT CCTTTTTGGC TGTTTGCCAG 
CAGTGCCTGT CTAATGTTAA TACTCCAGTG AAAGAACAGG CTTTCATGTT ACTCTGTGAT 
CTTCTGATGA TTTTCAGCCA CCAATTAATG ACAGGTGGCA GAGAGGGCCT TCAGCCTTTG 
GTGTTCAATC CAGATACTGG ACTCCAATCT GAACTCCTCA GTTTTGTGAT GGATCACGTT 
TTTATTGACC AAGACGAGGA GAACCAGAGC ATGGAGGGTG ATGAAGAAGA TGAAGCTAAT 
AAAATTGAGG CCTTACATAA AAGAAGGAAT CTACTTGCTG CTTTCAGCAA ACTTATCATT 
TATGACATTG TTGACATGCA TGCAGCTGCA GACATCTTCA AACACTACAT GAAGTATTAC 
AATGACTATG GTGATATTAT TAAGGAAACA CTGAGTAAAA CCAGGCAGAT TGATAAAATT 
CAGTGTGCCA AGACTCTCAT TCTCAGTTTG CAACAGTTAT TTAATGAACT TGTTCAAGAG 
CAAGGTCCCA ACCTAGATAG GACATCTGCC CATGTCAGTG GCATTAAAGA ACTGGCACGT 
CGCTTTGCCC TTACATTTGG ATTGGACCAG ATTAAGACAC GAGAAGCAGT TGCCACACTT 
CACAAGGATG GCATAGAGTT TGCATTTAAA TACCAAAATC AGAAAGGACA AGAGTATCCA 
CCTCCTAATC TGGCTTTTCT TGAAGTACTA AGTGAATTTT CTTCTAAACT TCTTCGACAG 
GACAAAAAGA CAGTTCATTC ATACCTAGAG AAATTCCTTA CCGAGCAGAT GATGGAAAGG 
AGGGAGGATG TATGGCTTCC ACTCATCTCC TATAGAAATT CATTAGTCAC TGGGGGTGAA 
GATGATAGAA TGTCTGTGAA CAGTGGAAGT AGCAGCAGCA AAACCTCATC AGTAAGGAAT 
AAGAAAGGAC GACCTCCACT TCATAAAAAA CGAGTAGAAG ATGAGAGTCT GGATAACACA 
TGGCTAAACA GGACTGACAC CATGATTCAG ACTCCTGGCC CCCTGCCAGC ACCACAACTC 
ACATCCACTG TACTGCGGGA GAACAGTCGG CCCATGGGAG ACCAGATTCA AGAACCTGAG 
TCTGAACATG GTTCTGAACC AGACTTTTTA CACAATCCTC AGATGCAGAT CTCTTGGTTA 
GGCCAGCCGA AGTTAGAAGA CTTAAATCGG AAGGACAGAA CAGGAATGAA CTACATGAAA 
GTGAGAACTG GAGTGAGGCA TGCTGTTCGG GGTCTAATGG AGGAAGATGC TGAGCCCATC 
TTTGAAGATG TGATGATGTC ATCCCGAAGC CAGTTAGAAG ATATGAATGA AGAATTTGAG 
GACACCATGG TTATTGATCT GCCTCCATCA AGAAATCGGC GAGAGAGAGC TGAGCTAAGG 
CCAGACTTCT TTGACTCTGC AGCTATCATA GAAGATGATT CAGGATTTGG AATGCCTATG 
TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

STAG1 ( XM_017005524.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016861013.1 Homo sapiens stromal antigen 1 (STAG1), transcript variant X4, mRNA.

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CloneID	OHu63832
Clone ID Related Accession (Same CDS sequence)	XM_017005524.1 , XM_017005524.2
Accession Version	XM_017005524.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3099bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	cohesin subunit SA-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTCA TGACTGCTCT GGTGAATGTT GCCTTAAACC TCAGTATTCA TCAGGATAAT 
ACCCAGAGAC AATATGAAGC CGAGAGAAAT AAAATGATTG GGAAGAGAGC CAATGAAAGG 
TTGGAGTTAC TACTTCAGAA ACGCAAAGAG CTGCAAGAAA ATCAGGATGA AATCGAAAAT 
ATGATGAACT CTATTTTTAA GGGTATATTT GTTCATAGAT ACCGTGATGC TATTGCTGAG 
ATTAGAGCCA TTTGTATTGA AGAAATTGGA GTATGGATGA AAATGTATAG TGATGCCTTC 
CTAAATGACA GTTACCTAAA ATATGTTGGC TGGACTCTTC ATGACAGGCA AGGGGAAGTC 
AGGCTGAAGT GTTTGAAAGC TCTGCAGAGT CTATATACCA ATAGAGAATT ATTCCCCAAA 
TTGGAACTAT TCACTAACCG ATTCAAGGAT CGCATTGTAT CAATGACACT TGATAAAGAA 
TATGATGTTG CTGTGGAAGC TATTCGATTG GTTACTCTGA TACTTCATGG AAGTGAAGAA 
GCTCTTTCCA ATGAAGACTG TGAAAATGTT TACCACTTGG TGTACTCGGC ACATCGCCCT 
GTTGCTGTGG CAGCTGGAGA GTTCCTTCAC AAAAAGCTAT TTAGCAGACA TGACCCACAA 
GCAGAAGAAG CATTAGCAAA GAGGAGGGGA AGAAACAGCC CGAATGGAAA CCTCATTAGG 
ATGCTGGTTC TTTTCTTTCT TGAAAGTGAG TTACATGAAC ATGCAGCCTA CTTGGTGGAC 
AGTTTATGGG AGAGCTCTCA AGAACTGTTG AAAGACTGGG AATGTATGAC AGAGTTGCTA 
TTAGAAGAAC CTGTTCAAGG AGAGGAAGCA ATGTCTGATC GTCAAGAGAG TGCTCTTATA 
GAGCTAATGG TTTGTACAAT TCGTCAAGCT GCTGAGGCAC ATCCTCCAGT GGGAAGGGGT 
ACCGGCAAGA GAGTGCTAAC TGCCAAAGAA AGGAAAACTC AAATTGATGA TAGAAACAAA 
TTGACTGAAC ATTTTATTAT TACACTTCCT ATGTTACTGT CAAAGTATTC TGCAGATGCA 
GAGAAGGTAG CAAACTTGCT ACAAATCCCA CAGTATTTTG ATTTAGAAAT CTACAGCACA 
GGTAGAATGG AAAAGCATCT GGATGCTTTA TTAAAACAGA TTAAGTTTGT TGTGGAGAAA 
CACGTAGAAT CAGATGTTCT AGAAGCCTGC AGTAAAACCT ATAGTATCTT ATGCAGTGAA 
GAATATACCA TCCAGAACAG AGTTGACATA GCTCGAAGCC AGCTGATTGA TGAGTTTGTA 
GATCGATTCA ATCATTCTGT GGAAGACCTA TTGCAAGAGG GAGAAGAAGC TGATGATGAT 
GACATTTACA ATGTTCTTTC TACATTAAAG CGGTTAACTT CTTTTCACAA TGCACATGAT 
CTCACAAAAT GGGATCTCTT TGGTAATTGC TACAGATTAT TGAAGACTGG AATTGAACAT 
GGAGCCATGC CAGAACAGAT AGTCGTGCAA GCACTGCAGT GTTCCCATTA TTCGATTCTT 
TGGCAGTTGG TGAAAATTAC TGATGGCTCT CCTTCCAAAG AGGATTTGTT GGTATTGAGG 
AAAACGGTGA AATCCTTTTT GGCTGTTTGC CAGCAGTGCC TGTCTAATGT TAATACTCCA 
GTGAAAGAAC AGGCTTTCAT GTTACTCTGT GATCTTCTGA TGATTTTCAG CCACCAATTA 
ATGACAGGTG GCAGAGAGGG CCTTCAGCCT TTGGTGTTCA ATCCAGATAC TGGACTCCAA 
TCTGAACTCC TCAGTTTTGT GATGGATCAC GTTTTTATTG ACCAAGACGA GGAGAACCAG 
AGCATGGAGG GTGATGAAGA AGATGAAGCT AATAAAATTG AGGCCTTACA TAAAAGAAGG 
AATCTACTTG CTGCTTTCAG CAAACTTATC ATTTATGACA TTGTTGACAT GCATGCAGCT 
GCAGACATCT TCAAACACTA CATGAAGTAT TACAATGACT ATGGTGATAT TATTAAGGAA 
ACACTGAGTA AAACCAGGCA GATTGATAAA ATTCAGTGTG CCAAGACTCT CATTCTCAGT 
TTGCAACAGT TATTTAATGA ACTTGTTCAA GAGCAAGGTC CCAACCTAGA TAGGACATCT 
GCCCATGTCA GTGGCATTAA AGAACTGGCA CGTCGCTTTG CCCTTACATT TGGATTGGAC 
CAGATTAAGA CACGAGAAGC AGTTGCCACA CTTCACAAGG ATGGCATAGA GTTTGCATTT 
AAATACCAAA ATCAGAAAGG ACAAGAGTAT CCACCTCCTA ATCTGGCTTT TCTTGAAGTA 
CTAAGTGAAT TTTCTTCTAA ACTTCTTCGA CAGGACAAAA AGACAGTTCA TTCATACCTA 
GAGAAATTCC TTACCGAGCA GATGATGGAA AGGAGGGAGG ATGTATGGCT TCCACTCATC 
TCCTATAGAA ATTCATTAGT CACTGGGGGT GAAGATGATA GAATGTCTGT GAACAGTGGA 
AGTAGCAGCA GCAAAACCTC ATCAGTAAGG AATAAGAAAG GACGACCTCC ACTTCATAAA 
AAACGAGTAG AAGATGAGAG TCTGGATAAC ACATGGCTAA ACAGGACTGA CACCATGATT 
CAGACTCCTG GCCCCCTGCC AGCACCACAA CTCACATCCA CTGTACTGCG GGAGAACAGT 
CGGCCCATGG GAGACCAGAT TCAAGAACCT GAGTCTGAAC ATGGTTCTGA ACCAGACTTT 
TTACACAATC CTCAGATGCA GATCTCTTGG TTAGGCCAGC CGAAGTTAGA AGACTTAAAT 
CGGAAGGACA GAACAGGAAT GAACTACATG AAAGTGAGAA CTGGAGTGAG GCATGCTGTT 
CGGGGTCTAA TGGAGGAAGA TGCTGAGCCC ATCTTTGAAG ATGTGATGAT GTCATCCCGA 
AGCCAGTTAG AAGATATGAA TGAAGAATTT GAGGACACCA TGGTTATTGA TCTGCCTCCA 
TCAAGAAATC GGCGAGAGAG AGCTGAGCTA AGGCCAGACT TCTTTGACTC TGCAGCTATC 
ATAGAAGATG ATTCAGGATT TGGAATGCCT ATGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016861013.1
CDS	1045..4143
Translation	MKLMTALVNVALNLSIHQDNTQRQYEAERNKMIGKRANERLELLLQKRKELQENQDEIENMMNSIFKGIFVHRYRDAIAEIRAICIEEIGVWMKMYSDAFLNDSYLKYVGWTLHDRQGEVRLKCLKALQSLYTNRELFPKLELFTNRFKDRIVSMTLDKEYDVAVEAIRLVTLILHGSEEALSNEDCENVYHLVYSAHRPVAVAAGEFLHKKLFSRHDPQAEEALAKRRGRNSPNGNLIRMLVLFFLESELHEHAAYLVDSLWESSQELLKDWECMTELLLEEPVQGEEAMSDRQESALIELMVCTIRQAAEAHPPVGRGTGKRVLTAKERKTQIDDRNKLTEHFIITLPMLLSKYSADAEKVANLLQIPQYFDLEIYSTGRMEKHLDALLKQIKFVVEKHVESDVLEACSKTYSILCSEEYTIQNRVDIARSQLIDEFVDRFNHSVEDLLQEGEEADDDDIYNVLSTLKRLTSFHNAHDLTKWDLFGNCYRLLKTGIEHGAMPEQIVVQALQCSHYSILWQLVKITDGSPSKEDLLVLRKTVKSFLAVCQQCLSNVNTPVKEQAFMLLCDLLMIFSHQLMTGGREGLQPLVFNPDTGLQSELLSFVMDHVFIDQDEENQSMEGDEEDEANKIEALHKRRNLLAAFSKLIIYDIVDMHAAADIFKHYMKYYNDYGDIIKETLSKTRQIDKIQCAKTLILSLQQLFNELVQEQGPNLDRTSAHVSGIKELARRFALTFGLDQIKTREAVATLHKDGIEFAFKYQNQKGQEYPPPNLAFLEVLSEFSSKLLRQDKKTVHSYLEKFLTEQMMERREDVWLPLISYRNSLVTGGEDDRMSVNSGSSSSKTSSVRNKKGRPPLHKKRVEDESLDNTWLNRTDTMIQTPGPLPAPQLTSTVLRENSRPMGDQIQEPESEHGSEPDFLHNPQMQISWLGQPKLEDLNRKDRTGMNYMKVRTGVRHAVRGLMEEDAEPIFEDVMMSSRSQLEDMNEEFEDTMVIDLPPSRNRRERAELRPDFFDSAAIIEDDSGFGMPMF

Target ORF information:

RefSeq Version	XM_017005524.1
Organism	Homo sapiens(human)
Definition	Homo sapiens stromal antigen 1 (STAG1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017005524.1

ORF Insert Sequence:

ATGAAGCTCA TGACTGCTCT GGTGAATGTT GCCTTAAACC TCAGTATTCA TCAGGATAAT 
ACCCAGAGAC AATATGAAGC CGAGAGAAAT AAAATGATTG GGAAGAGAGC CAATGAAAGG 
TTGGAGTTAC TACTTCAGAA ACGCAAAGAG CTGCAAGAAA ATCAGGATGA AATCGAAAAT 
ATGATGAACT CTATTTTTAA GGGTATATTT GTTCATAGAT ACCGTGATGC TATTGCTGAG 
ATTAGAGCCA TTTGTATTGA AGAAATTGGA GTATGGATGA AAATGTATAG TGATGCCTTC 
CTAAATGACA GTTACCTAAA ATATGTTGGC TGGACTCTTC ATGACAGGCA AGGGGAAGTC 
AGGCTGAAGT GTTTGAAAGC TCTGCAGAGT CTATATACCA ATAGAGAATT ATTCCCCAAA 
TTGGAACTAT TCACTAACCG ATTCAAGGAT CGCATTGTAT CAATGACACT TGATAAAGAA 
TATGATGTTG CTGTGGAAGC TATTCGATTG GTTACTCTGA TACTTCATGG AAGTGAAGAA 
GCTCTTTCCA ATGAAGACTG TGAAAATGTT TACCACTTGG TGTACTCGGC ACATCGCCCT 
GTTGCTGTGG CAGCTGGAGA GTTCCTTCAC AAAAAGCTAT TTAGCAGACA TGACCCACAA 
GCAGAAGAAG CATTAGCAAA GAGGAGGGGA AGAAACAGCC CGAATGGAAA CCTCATTAGG 
ATGCTGGTTC TTTTCTTTCT TGAAAGTGAG TTACATGAAC ATGCAGCCTA CTTGGTGGAC 
AGTTTATGGG AGAGCTCTCA AGAACTGTTG AAAGACTGGG AATGTATGAC AGAGTTGCTA 
TTAGAAGAAC CTGTTCAAGG AGAGGAAGCA ATGTCTGATC GTCAAGAGAG TGCTCTTATA 
GAGCTAATGG TTTGTACAAT TCGTCAAGCT GCTGAGGCAC ATCCTCCAGT GGGAAGGGGT 
ACCGGCAAGA GAGTGCTAAC TGCCAAAGAA AGGAAAACTC AAATTGATGA TAGAAACAAA 
TTGACTGAAC ATTTTATTAT TACACTTCCT ATGTTACTGT CAAAGTATTC TGCAGATGCA 
GAGAAGGTAG CAAACTTGCT ACAAATCCCA CAGTATTTTG ATTTAGAAAT CTACAGCACA 
GGTAGAATGG AAAAGCATCT GGATGCTTTA TTAAAACAGA TTAAGTTTGT TGTGGAGAAA 
CACGTAGAAT CAGATGTTCT AGAAGCCTGC AGTAAAACCT ATAGTATCTT ATGCAGTGAA 
GAATATACCA TCCAGAACAG AGTTGACATA GCTCGAAGCC AGCTGATTGA TGAGTTTGTA 
GATCGATTCA ATCATTCTGT GGAAGACCTA TTGCAAGAGG GAGAAGAAGC TGATGATGAT 
GACATTTACA ATGTTCTTTC TACATTAAAG CGGTTAACTT CTTTTCACAA TGCACATGAT 
CTCACAAAAT GGGATCTCTT TGGTAATTGC TACAGATTAT TGAAGACTGG AATTGAACAT 
GGAGCCATGC CAGAACAGAT AGTCGTGCAA GCACTGCAGT GTTCCCATTA TTCGATTCTT 
TGGCAGTTGG TGAAAATTAC TGATGGCTCT CCTTCCAAAG AGGATTTGTT GGTATTGAGG 
AAAACGGTGA AATCCTTTTT GGCTGTTTGC CAGCAGTGCC TGTCTAATGT TAATACTCCA 
GTGAAAGAAC AGGCTTTCAT GTTACTCTGT GATCTTCTGA TGATTTTCAG CCACCAATTA 
ATGACAGGTG GCAGAGAGGG CCTTCAGCCT TTGGTGTTCA ATCCAGATAC TGGACTCCAA 
TCTGAACTCC TCAGTTTTGT GATGGATCAC GTTTTTATTG ACCAAGACGA GGAGAACCAG 
AGCATGGAGG GTGATGAAGA AGATGAAGCT AATAAAATTG AGGCCTTACA TAAAAGAAGG 
AATCTACTTG CTGCTTTCAG CAAACTTATC ATTTATGACA TTGTTGACAT GCATGCAGCT 
GCAGACATCT TCAAACACTA CATGAAGTAT TACAATGACT ATGGTGATAT TATTAAGGAA 
ACACTGAGTA AAACCAGGCA GATTGATAAA ATTCAGTGTG CCAAGACTCT CATTCTCAGT 
TTGCAACAGT TATTTAATGA ACTTGTTCAA GAGCAAGGTC CCAACCTAGA TAGGACATCT 
GCCCATGTCA GTGGCATTAA AGAACTGGCA CGTCGCTTTG CCCTTACATT TGGATTGGAC 
CAGATTAAGA CACGAGAAGC AGTTGCCACA CTTCACAAGG ATGGCATAGA GTTTGCATTT 
AAATACCAAA ATCAGAAAGG ACAAGAGTAT CCACCTCCTA ATCTGGCTTT TCTTGAAGTA 
CTAAGTGAAT TTTCTTCTAA ACTTCTTCGA CAGGACAAAA AGACAGTTCA TTCATACCTA 
GAGAAATTCC TTACCGAGCA GATGATGGAA AGGAGGGAGG ATGTATGGCT TCCACTCATC 
TCCTATAGAA ATTCATTAGT CACTGGGGGT GAAGATGATA GAATGTCTGT GAACAGTGGA 
AGTAGCAGCA GCAAAACCTC ATCAGTAAGG AATAAGAAAG GACGACCTCC ACTTCATAAA 
AAACGAGTAG AAGATGAGAG TCTGGATAAC ACATGGCTAA ACAGGACTGA CACCATGATT 
CAGACTCCTG GCCCCCTGCC AGCACCACAA CTCACATCCA CTGTACTGCG GGAGAACAGT 
CGGCCCATGG GAGACCAGAT TCAAGAACCT GAGTCTGAAC ATGGTTCTGA ACCAGACTTT 
TTACACAATC CTCAGATGCA GATCTCTTGG TTAGGCCAGC CGAAGTTAGA AGACTTAAAT 
CGGAAGGACA GAACAGGAAT GAACTACATG AAAGTGAGAA CTGGAGTGAG GCATGCTGTT 
CGGGGTCTAA TGGAGGAAGA TGCTGAGCCC ATCTTTGAAG ATGTGATGAT GTCATCCCGA 
AGCCAGTTAG AAGATATGAA TGAAGAATTT GAGGACACCA TGGTTATTGA TCTGCCTCCA 
TCAAGAAATC GGCGAGAGAG AGCTGAGCTA AGGCCAGACT TCTTTGACTC TGCAGCTATC 
ATAGAAGATG ATTCAGGATT TGGAATGCCT ATGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

STAG1 ( XM_017005524.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016861013.1 Homo sapiens stromal antigen 1 (STAG1), transcript variant X4, mRNA.

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CloneID	OHu63832
Clone ID Related Accession (Same CDS sequence)	XM_017005524.1 , XM_017005524.2
Accession Version	XM_017005524.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3099bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	cohesin subunit SA-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017005524.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTCA TGACTGCTCT GGTGAATGTT GCCTTAAACC TCAGTATTCA TCAGGATAAT 
ACCCAGAGAC AATATGAAGC CGAGAGAAAT AAAATGATTG GGAAGAGAGC CAATGAAAGG 
TTGGAGTTAC TACTTCAGAA ACGCAAAGAG CTGCAAGAAA ATCAGGATGA AATCGAAAAT 
ATGATGAACT CTATTTTTAA GGGTATATTT GTTCATAGAT ACCGTGATGC TATTGCTGAG 
ATTAGAGCCA TTTGTATTGA AGAAATTGGA GTATGGATGA AAATGTATAG TGATGCCTTC 
CTAAATGACA GTTACCTAAA ATATGTTGGC TGGACTCTTC ATGACAGGCA AGGGGAAGTC 
AGGCTGAAGT GTTTGAAAGC TCTGCAGAGT CTATATACCA ATAGAGAATT ATTCCCCAAA 
TTGGAACTAT TCACTAACCG ATTCAAGGAT CGCATTGTAT CAATGACACT TGATAAAGAA 
TATGATGTTG CTGTGGAAGC TATTCGATTG GTTACTCTGA TACTTCATGG AAGTGAAGAA 
GCTCTTTCCA ATGAAGACTG TGAAAATGTT TACCACTTGG TGTACTCGGC ACATCGCCCT 
GTTGCTGTGG CAGCTGGAGA GTTCCTTCAC AAAAAGCTAT TTAGCAGACA TGACCCACAA 
GCAGAAGAAG CATTAGCAAA GAGGAGGGGA AGAAACAGCC CGAATGGAAA CCTCATTAGG 
ATGCTGGTTC TTTTCTTTCT TGAAAGTGAG TTACATGAAC ATGCAGCCTA CTTGGTGGAC 
AGTTTATGGG AGAGCTCTCA AGAACTGTTG AAAGACTGGG AATGTATGAC AGAGTTGCTA 
TTAGAAGAAC CTGTTCAAGG AGAGGAAGCA ATGTCTGATC GTCAAGAGAG TGCTCTTATA 
GAGCTAATGG TTTGTACAAT TCGTCAAGCT GCTGAGGCAC ATCCTCCAGT GGGAAGGGGT 
ACCGGCAAGA GAGTGCTAAC TGCCAAAGAA AGGAAAACTC AAATTGATGA TAGAAACAAA 
TTGACTGAAC ATTTTATTAT TACACTTCCT ATGTTACTGT CAAAGTATTC TGCAGATGCA 
GAGAAGGTAG CAAACTTGCT ACAAATCCCA CAGTATTTTG ATTTAGAAAT CTACAGCACA 
GGTAGAATGG AAAAGCATCT GGATGCTTTA TTAAAACAGA TTAAGTTTGT TGTGGAGAAA 
CACGTAGAAT CAGATGTTCT AGAAGCCTGC AGTAAAACCT ATAGTATCTT ATGCAGTGAA 
GAATATACCA TCCAGAACAG AGTTGACATA GCTCGAAGCC AGCTGATTGA TGAGTTTGTA 
GATCGATTCA ATCATTCTGT GGAAGACCTA TTGCAAGAGG GAGAAGAAGC TGATGATGAT 
GACATTTACA ATGTTCTTTC TACATTAAAG CGGTTAACTT CTTTTCACAA TGCACATGAT 
CTCACAAAAT GGGATCTCTT TGGTAATTGC TACAGATTAT TGAAGACTGG AATTGAACAT 
GGAGCCATGC CAGAACAGAT AGTCGTGCAA GCACTGCAGT GTTCCCATTA TTCGATTCTT 
TGGCAGTTGG TGAAAATTAC TGATGGCTCT CCTTCCAAAG AGGATTTGTT GGTATTGAGG 
AAAACGGTGA AATCCTTTTT GGCTGTTTGC CAGCAGTGCC TGTCTAATGT TAATACTCCA 
GTGAAAGAAC AGGCTTTCAT GTTACTCTGT GATCTTCTGA TGATTTTCAG CCACCAATTA 
ATGACAGGTG GCAGAGAGGG CCTTCAGCCT TTGGTGTTCA ATCCAGATAC TGGACTCCAA 
TCTGAACTCC TCAGTTTTGT GATGGATCAC GTTTTTATTG ACCAAGACGA GGAGAACCAG 
AGCATGGAGG GTGATGAAGA AGATGAAGCT AATAAAATTG AGGCCTTACA TAAAAGAAGG 
AATCTACTTG CTGCTTTCAG CAAACTTATC ATTTATGACA TTGTTGACAT GCATGCAGCT 
GCAGACATCT TCAAACACTA CATGAAGTAT TACAATGACT ATGGTGATAT TATTAAGGAA 
ACACTGAGTA AAACCAGGCA GATTGATAAA ATTCAGTGTG CCAAGACTCT CATTCTCAGT 
TTGCAACAGT TATTTAATGA ACTTGTTCAA GAGCAAGGTC CCAACCTAGA TAGGACATCT 
GCCCATGTCA GTGGCATTAA AGAACTGGCA CGTCGCTTTG CCCTTACATT TGGATTGGAC 
CAGATTAAGA CACGAGAAGC AGTTGCCACA CTTCACAAGG ATGGCATAGA GTTTGCATTT 
AAATACCAAA ATCAGAAAGG ACAAGAGTAT CCACCTCCTA ATCTGGCTTT TCTTGAAGTA 
CTAAGTGAAT TTTCTTCTAA ACTTCTTCGA CAGGACAAAA AGACAGTTCA TTCATACCTA 
GAGAAATTCC TTACCGAGCA GATGATGGAA AGGAGGGAGG ATGTATGGCT TCCACTCATC 
TCCTATAGAA ATTCATTAGT CACTGGGGGT GAAGATGATA GAATGTCTGT GAACAGTGGA 
AGTAGCAGCA GCAAAACCTC ATCAGTAAGG AATAAGAAAG GACGACCTCC ACTTCATAAA 
AAACGAGTAG AAGATGAGAG TCTGGATAAC ACATGGCTAA ACAGGACTGA CACCATGATT 
CAGACTCCTG GCCCCCTGCC AGCACCACAA CTCACATCCA CTGTACTGCG GGAGAACAGT 
CGGCCCATGG GAGACCAGAT TCAAGAACCT GAGTCTGAAC ATGGTTCTGA ACCAGACTTT 
TTACACAATC CTCAGATGCA GATCTCTTGG TTAGGCCAGC CGAAGTTAGA AGACTTAAAT 
CGGAAGGACA GAACAGGAAT GAACTACATG AAAGTGAGAA CTGGAGTGAG GCATGCTGTT 
CGGGGTCTAA TGGAGGAAGA TGCTGAGCCC ATCTTTGAAG ATGTGATGAT GTCATCCCGA 
AGCCAGTTAG AAGATATGAA TGAAGAATTT GAGGACACCA TGGTTATTGA TCTGCCTCCA 
TCAAGAAATC GGCGAGAGAG AGCTGAGCTA AGGCCAGACT TCTTTGACTC TGCAGCTATC 
ATAGAAGATG ATTCAGGATT TGGAATGCCT ATGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016861013.1
CDS	1045..4143
Translation	MKLMTALVNVALNLSIHQDNTQRQYEAERNKMIGKRANERLELLLQKRKELQENQDEIENMMNSIFKGIFVHRYRDAIAEIRAICIEEIGVWMKMYSDAFLNDSYLKYVGWTLHDRQGEVRLKCLKALQSLYTNRELFPKLELFTNRFKDRIVSMTLDKEYDVAVEAIRLVTLILHGSEEALSNEDCENVYHLVYSAHRPVAVAAGEFLHKKLFSRHDPQAEEALAKRRGRNSPNGNLIRMLVLFFLESELHEHAAYLVDSLWESSQELLKDWECMTELLLEEPVQGEEAMSDRQESALIELMVCTIRQAAEAHPPVGRGTGKRVLTAKERKTQIDDRNKLTEHFIITLPMLLSKYSADAEKVANLLQIPQYFDLEIYSTGRMEKHLDALLKQIKFVVEKHVESDVLEACSKTYSILCSEEYTIQNRVDIARSQLIDEFVDRFNHSVEDLLQEGEEADDDDIYNVLSTLKRLTSFHNAHDLTKWDLFGNCYRLLKTGIEHGAMPEQIVVQALQCSHYSILWQLVKITDGSPSKEDLLVLRKTVKSFLAVCQQCLSNVNTPVKEQAFMLLCDLLMIFSHQLMTGGREGLQPLVFNPDTGLQSELLSFVMDHVFIDQDEENQSMEGDEEDEANKIEALHKRRNLLAAFSKLIIYDIVDMHAAADIFKHYMKYYNDYGDIIKETLSKTRQIDKIQCAKTLILSLQQLFNELVQEQGPNLDRTSAHVSGIKELARRFALTFGLDQIKTREAVATLHKDGIEFAFKYQNQKGQEYPPPNLAFLEVLSEFSSKLLRQDKKTVHSYLEKFLTEQMMERREDVWLPLISYRNSLVTGGEDDRMSVNSGSSSSKTSSVRNKKGRPPLHKKRVEDESLDNTWLNRTDTMIQTPGPLPAPQLTSTVLRENSRPMGDQIQEPESEHGSEPDFLHNPQMQISWLGQPKLEDLNRKDRTGMNYMKVRTGVRHAVRGLMEEDAEPIFEDVMMSSRSQLEDMNEEFEDTMVIDLPPSRNRRERAELRPDFFDSAAIIEDDSGFGMPMF

Target ORF information:

RefSeq Version	XM_017005524.2
Organism	Homo sapiens(human)
Definition	Homo sapiens stromal antigen 1 (STAG1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017005524.2

ORF Insert Sequence:

ATGAAGCTCA TGACTGCTCT GGTGAATGTT GCCTTAAACC TCAGTATTCA TCAGGATAAT 
ACCCAGAGAC AATATGAAGC CGAGAGAAAT AAAATGATTG GGAAGAGAGC CAATGAAAGG 
TTGGAGTTAC TACTTCAGAA ACGCAAAGAG CTGCAAGAAA ATCAGGATGA AATCGAAAAT 
ATGATGAACT CTATTTTTAA GGGTATATTT GTTCATAGAT ACCGTGATGC TATTGCTGAG 
ATTAGAGCCA TTTGTATTGA AGAAATTGGA GTATGGATGA AAATGTATAG TGATGCCTTC 
CTAAATGACA GTTACCTAAA ATATGTTGGC TGGACTCTTC ATGACAGGCA AGGGGAAGTC 
AGGCTGAAGT GTTTGAAAGC TCTGCAGAGT CTATATACCA ATAGAGAATT ATTCCCCAAA 
TTGGAACTAT TCACTAACCG ATTCAAGGAT CGCATTGTAT CAATGACACT TGATAAAGAA 
TATGATGTTG CTGTGGAAGC TATTCGATTG GTTACTCTGA TACTTCATGG AAGTGAAGAA 
GCTCTTTCCA ATGAAGACTG TGAAAATGTT TACCACTTGG TGTACTCGGC ACATCGCCCT 
GTTGCTGTGG CAGCTGGAGA GTTCCTTCAC AAAAAGCTAT TTAGCAGACA TGACCCACAA 
GCAGAAGAAG CATTAGCAAA GAGGAGGGGA AGAAACAGCC CGAATGGAAA CCTCATTAGG 
ATGCTGGTTC TTTTCTTTCT TGAAAGTGAG TTACATGAAC ATGCAGCCTA CTTGGTGGAC 
AGTTTATGGG AGAGCTCTCA AGAACTGTTG AAAGACTGGG AATGTATGAC AGAGTTGCTA 
TTAGAAGAAC CTGTTCAAGG AGAGGAAGCA ATGTCTGATC GTCAAGAGAG TGCTCTTATA 
GAGCTAATGG TTTGTACAAT TCGTCAAGCT GCTGAGGCAC ATCCTCCAGT GGGAAGGGGT 
ACCGGCAAGA GAGTGCTAAC TGCCAAAGAA AGGAAAACTC AAATTGATGA TAGAAACAAA 
TTGACTGAAC ATTTTATTAT TACACTTCCT ATGTTACTGT CAAAGTATTC TGCAGATGCA 
GAGAAGGTAG CAAACTTGCT ACAAATCCCA CAGTATTTTG ATTTAGAAAT CTACAGCACA 
GGTAGAATGG AAAAGCATCT GGATGCTTTA TTAAAACAGA TTAAGTTTGT TGTGGAGAAA 
CACGTAGAAT CAGATGTTCT AGAAGCCTGC AGTAAAACCT ATAGTATCTT ATGCAGTGAA 
GAATATACCA TCCAGAACAG AGTTGACATA GCTCGAAGCC AGCTGATTGA TGAGTTTGTA 
GATCGATTCA ATCATTCTGT GGAAGACCTA TTGCAAGAGG GAGAAGAAGC TGATGATGAT 
GACATTTACA ATGTTCTTTC TACATTAAAG CGGTTAACTT CTTTTCACAA TGCACATGAT 
CTCACAAAAT GGGATCTCTT TGGTAATTGC TACAGATTAT TGAAGACTGG AATTGAACAT 
GGAGCCATGC CAGAACAGAT AGTCGTGCAA GCACTGCAGT GTTCCCATTA TTCGATTCTT 
TGGCAGTTGG TGAAAATTAC TGATGGCTCT CCTTCCAAAG AGGATTTGTT GGTATTGAGG 
AAAACGGTGA AATCCTTTTT GGCTGTTTGC CAGCAGTGCC TGTCTAATGT TAATACTCCA 
GTGAAAGAAC AGGCTTTCAT GTTACTCTGT GATCTTCTGA TGATTTTCAG CCACCAATTA 
ATGACAGGTG GCAGAGAGGG CCTTCAGCCT TTGGTGTTCA ATCCAGATAC TGGACTCCAA 
TCTGAACTCC TCAGTTTTGT GATGGATCAC GTTTTTATTG ACCAAGACGA GGAGAACCAG 
AGCATGGAGG GTGATGAAGA AGATGAAGCT AATAAAATTG AGGCCTTACA TAAAAGAAGG 
AATCTACTTG CTGCTTTCAG CAAACTTATC ATTTATGACA TTGTTGACAT GCATGCAGCT 
GCAGACATCT TCAAACACTA CATGAAGTAT TACAATGACT ATGGTGATAT TATTAAGGAA 
ACACTGAGTA AAACCAGGCA GATTGATAAA ATTCAGTGTG CCAAGACTCT CATTCTCAGT 
TTGCAACAGT TATTTAATGA ACTTGTTCAA GAGCAAGGTC CCAACCTAGA TAGGACATCT 
GCCCATGTCA GTGGCATTAA AGAACTGGCA CGTCGCTTTG CCCTTACATT TGGATTGGAC 
CAGATTAAGA CACGAGAAGC AGTTGCCACA CTTCACAAGG ATGGCATAGA GTTTGCATTT 
AAATACCAAA ATCAGAAAGG ACAAGAGTAT CCACCTCCTA ATCTGGCTTT TCTTGAAGTA 
CTAAGTGAAT TTTCTTCTAA ACTTCTTCGA CAGGACAAAA AGACAGTTCA TTCATACCTA 
GAGAAATTCC TTACCGAGCA GATGATGGAA AGGAGGGAGG ATGTATGGCT TCCACTCATC 
TCCTATAGAA ATTCATTAGT CACTGGGGGT GAAGATGATA GAATGTCTGT GAACAGTGGA 
AGTAGCAGCA GCAAAACCTC ATCAGTAAGG AATAAGAAAG GACGACCTCC ACTTCATAAA 
AAACGAGTAG AAGATGAGAG TCTGGATAAC ACATGGCTAA ACAGGACTGA CACCATGATT 
CAGACTCCTG GCCCCCTGCC AGCACCACAA CTCACATCCA CTGTACTGCG GGAGAACAGT 
CGGCCCATGG GAGACCAGAT TCAAGAACCT GAGTCTGAAC ATGGTTCTGA ACCAGACTTT 
TTACACAATC CTCAGATGCA GATCTCTTGG TTAGGCCAGC CGAAGTTAGA AGACTTAAAT 
CGGAAGGACA GAACAGGAAT GAACTACATG AAAGTGAGAA CTGGAGTGAG GCATGCTGTT 
CGGGGTCTAA TGGAGGAAGA TGCTGAGCCC ATCTTTGAAG ATGTGATGAT GTCATCCCGA 
AGCCAGTTAG AAGATATGAA TGAAGAATTT GAGGACACCA TGGTTATTGA TCTGCCTCCA 
TCAAGAAATC GGCGAGAGAG AGCTGAGCTA AGGCCAGACT TCTTTGACTC TGCAGCTATC 
ATAGAAGATG ATTCAGGATT TGGAATGCCT ATGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

STAG1 ( NM_005862.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_005853.2 Homo sapiens stromal antigen 1 (STAG1), mRNA.

Price & Availability↑

CloneID	OHu15240
Clone ID Related Accession (Same CDS sequence)	NM_005862.2 , NM_005862.3
Accession Version	NM_005862.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3777bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-13
Organism	Homo sapiens(human)
Product	cohesin subunit SA-1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AU132652.1, BC064699.1 and BC017735.1. On Apr 6, 2005 this sequence version replaced NM_005862.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.236213.1, SRR1803613.141831.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGATTACTT CAGAATTACC AGTGTTACAG GATTCAACTA ATGAAACTAC TGCCCATTCC 
GATGCTGGCA GCGAGCTTGA AGAAACAGAG GTCAAAGGAA AAAGAAAAAG GGGTCGTCCT 
GGCCGGCCTC CATCTACAAA TAAGAAACCT CGAAAATCTC CAGGTGAGAA GAGCAGAATT 
GAAGCTGGAA TTAGAGGAGC AGGCCGTGGA AGAGCTAATG GACACCCTCA ACAGAATGGG 
GAAGGGGAGC CTGTCACATT ATTTGAGGTG GTGAAACTGG GGAAAAGTGC AATGCAGTCC 
GTGGTGGATG ACTGGATTGA ATCATATAAA CAAGACAGGG ACATCGCACT TCTGGATTTA 
ATCAACTTTT TTATCCAGTG TTCAGGATGT CGAGGTACTG TGAGAATAGA GATGTTTCGA 
AATATGCAGA ATGCAGAAAT CATCAGAAAA ATGACTGAAG AATTTGATGA GGACAGTGGT 
GATTATCCTC TTACCATGCC TGGACCTCAG TGGAAAAAAT TTCGTTCAAA CTTTTGTGAA 
TTTATTGGAG TCCTGATTCG ACAGTGTCAG TATAGCATAA TTTATGATGA GTATATGATG 
GACACAGTAA TCTCCCTTTT GACGGGTTTG TCAGACTCCC AGGTCAGAGC TTTTAGGCAT 
ACAAGTACCC TGGCTGCCAT GAAGCTCATG ACTGCTCTGG TGAATGTTGC CTTAAACCTC 
AGTATTCATC AGGATAATAC CCAGAGACAA TATGAAGCCG AGAGAAATAA AATGATTGGG 
AAGAGAGCCA ATGAAAGGTT GGAGTTACTA CTTCAGAAAC GCAAAGAGCT GCAAGAAAAT 
CAGGATGAAA TCGAAAATAT GATGAACTCT ATTTTTAAGG GTATATTTGT TCATAGATAC 
CGTGATGCTA TTGCTGAGAT TAGAGCCATT TGTATTGAAG AAATTGGAGT ATGGATGAAA 
ATGTATAGTG ATGCCTTCCT AAATGACAGT TACCTAAAAT ATGTTGGCTG GACTCTTCAT 
GACAGGCAAG GGGAAGTCAG GCTGAAGTGT TTGAAAGCTC TGCAGAGTCT ATATACCAAT 
AGAGAATTAT TCCCCAAATT GGAACTATTC ACTAACCGAT TCAAGGATCG CATTGTATCA 
ATGACACTTG ATAAAGAATA TGATGTTGCT GTGGAAGCTA TTCGATTGGT TACTCTGATA 
CTTCATGGAA GTGAAGAAGC TCTTTCCAAT GAAGACTGTG AAAATGTTTA CCACTTGGTG 
TACTCGGCAC ATCGCCCTGT TGCTGTGGCA GCTGGAGAGT TCCTTCACAA AAAGCTATTT 
AGCAGACATG ACCCACAAGC AGAAGAAGCA TTAGCAAAGA GGAGGGGAAG AAACAGCCCG 
AATGGAAACC TCATTAGGAT GCTGGTTCTT TTCTTTCTTG AAAGTGAGTT ACATGAACAT 
GCAGCCTACT TGGTGGACAG TTTATGGGAG AGCTCTCAAG AACTGTTGAA AGACTGGGAA 
TGTATGACAG AGTTGCTATT AGAAGAACCT GTTCAAGGAG AGGAAGCAAT GTCTGATCGT 
CAAGAGAGTG CTCTTATAGA GCTAATGGTT TGTACAATTC GTCAAGCTGC TGAGGCACAT 
CCTCCAGTGG GAAGGGGTAC CGGCAAGAGA GTGCTAACTG CCAAAGAAAG GAAAACTCAA 
ATTGATGATA GAAACAAATT GACTGAACAT TTTATTATTA CACTTCCTAT GTTACTGTCA 
AAGTATTCTG CAGATGCAGA GAAGGTAGCA AACTTGCTAC AAATCCCACA GTATTTTGAT 
TTAGAAATCT ACAGCACAGG TAGAATGGAA AAGCATCTGG ATGCTTTATT AAAACAGATT 
AAGTTTGTTG TGGAGAAACA CGTAGAATCA GATGTTCTAG AAGCCTGCAG TAAAACCTAT 
AGTATCTTAT GCAGTGAAGA ATATACCATC CAGAACAGAG TTGACATAGC TCGAAGCCAG 
CTGATTGATG AGTTTGTAGA TCGATTCAAT CATTCTGTGG AAGACCTATT GCAAGAGGGA 
GAAGAAGCTG ATGATGATGA CATTTACAAT GTTCTTTCTA CATTAAAGCG GTTAACTTCT 
TTTCACAATG CACATGATCT CACAAAATGG GATCTCTTTG GTAATTGCTA CAGATTATTG 
AAGACTGGAA TTGAACATGG AGCCATGCCA GAACAGATAG TCGTGCAAGC ACTGCAGTGT 
TCCCATTATT CGATTCTTTG GCAGTTGGTG AAAATTACTG ATGGCTCTCC TTCCAAAGAG 
GATTTGTTGG TATTGAGGAA AACGGTGAAA TCCTTTTTGG CTGTTTGCCA GCAGTGCCTG 
TCTAATGTTA ATACTCCAGT GAAAGAACAG GCTTTCATGT TACTCTGTGA TCTTCTGATG 
ATTTTCAGCC ACCAATTAAT GACAGGTGGC AGAGAGGGCC TTCAGCCTTT GGTGTTCAAT 
CCAGATACTG GACTCCAATC TGAACTCCTC AGTTTTGTGA TGGATCACGT TTTTATTGAC 
CAAGACGAGG AGAACCAGAG CATGGAGGGT GATGAAGAAG ATGAAGCTAA TAAAATTGAG 
GCCTTACATA AAAGAAGGAA TCTACTTGCT GCTTTCAGCA AACTTATCAT TTATGACATT 
GTTGACATGC ATGCAGCTGC AGACATCTTC AAACACTACA TGAAGTATTA CAATGACTAT 
GGTGATATTA TTAAGGAAAC ACTGAGTAAA ACCAGGCAGA TTGATAAAAT TCAGTGTGCC 
AAGACTCTCA TTCTCAGTTT GCAACAGTTA TTTAATGAAC TTGTTCAAGA GCAAGGTCCC 
AACCTAGATA GGACATCTGC CCATGTCAGT GGCATTAAAG AACTGGCACG TCGCTTTGCC 
CTTACATTTG GATTGGACCA GATTAAGACA CGAGAAGCAG TTGCCACACT TCACAAGGAT 
GGCATAGAGT TTGCATTTAA ATACCAAAAT CAGAAAGGAC AAGAGTATCC ACCTCCTAAT 
CTGGCTTTTC TTGAAGTACT AAGTGAATTT TCTTCTAAAC TTCTTCGACA GGACAAAAAG 
ACAGTTCATT CATACCTAGA GAAATTCCTT ACCGAGCAGA TGATGGAAAG GAGGGAGGAT 
GTATGGCTTC CACTCATCTC CTATAGAAAT TCATTAGTCA CTGGGGGTGA AGATGATAGA 
ATGTCTGTGA ACAGTGGAAG TAGCAGCAGC AAAACCTCAT CAGTAAGGAA TAAGAAAGGA 
CGACCTCCAC TTCATAAAAA ACGAGTAGAA GATGAGAGTC TGGATAACAC ATGGCTAAAC 
AGGACTGACA CCATGATTCA GACTCCTGGC CCCCTGCCAG CACCACAACT CACATCCACT 
GTACTGCGGG AGAACAGTCG GCCCATGGGA GACCAGATTC AAGAACCTGA GTCTGAACAT 
GGTTCTGAAC CAGACTTTTT ACACAATCCT CAGATGCAGA TCTCTTGGTT AGGCCAGCCG 
AAGTTAGAAG ACTTAAATCG GAAGGACAGA ACAGGAATGA ACTACATGAA AGTGAGAACT 
GGAGTGAGGC ATGCTGTTCG GGGTCTAATG GAGGAAGATG CTGAGCCCAT CTTTGAAGAT 
GTGATGATGT CATCCCGAAG CCAGTTAGAA GATATGAATG AAGAATTTGA GGACACCATG 
GTTATTGATC TGCCTCCATC AAGAAATCGG CGAGAGAGAG CTGAGCTAAG GCCAGACTTC 
TTTGACTCTG CAGCTATCAT AGAAGATGAT TCAGGATTTG GAATGCCTAT GTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005853.2
CDS	293..4069
Translation	MITSELPVLQDSTNETTAHSDAGSELEETEVKGKRKRGRPGRPPSTNKKPRKSPGEKSRIEAGIRGAGRGRANGHPQQNGEGEPVTLFEVVKLGKSAMQSVVDDWIESYKQDRDIALLDLINFFIQCSGCRGTVRIEMFRNMQNAEIIRKMTEEFDEDSGDYPLTMPGPQWKKFRSNFCEFIGVLIRQCQYSIIYDEYMMDTVISLLTGLSDSQVRAFRHTSTLAAMKLMTALVNVALNLSIHQDNTQRQYEAERNKMIGKRANERLELLLQKRKELQENQDEIENMMNSIFKGIFVHRYRDAIAEIRAICIEEIGVWMKMYSDAFLNDSYLKYVGWTLHDRQGEVRLKCLKALQSLYTNRELFPKLELFTNRFKDRIVSMTLDKEYDVAVEAIRLVTLILHGSEEALSNEDCENVYHLVYSAHRPVAVAAGEFLHKKLFSRHDPQAEEALAKRRGRNSPNGNLIRMLVLFFLESELHEHAAYLVDSLWESSQELLKDWECMTELLLEEPVQGEEAMSDRQESALIELMVCTIRQAAEAHPPVGRGTGKRVLTAKERKTQIDDRNKLTEHFIITLPMLLSKYSADAEKVANLLQIPQYFDLEIYSTGRMEKHLDALLKQIKFVVEKHVESDVLEACSKTYSILCSEEYTIQNRVDIARSQLIDEFVDRFNHSVEDLLQEGEEADDDDIYNVLSTLKRLTSFHNAHDLTKWDLFGNCYRLLKTGIEHGAMPEQIVVQALQCSHYSILWQLVKITDGSPSKEDLLVLRKTVKSFLAVCQQCLSNVNTPVKEQAFMLLCDLLMIFSHQLMTGGREGLQPLVFNPDTGLQSELLSFVMDHVFIDQDEENQSMEGDEEDEANKIEALHKRRNLLAAFSKLIIYDIVDMHAAADIFKHYMKYYNDYGDIIKETLSKTRQIDKIQCAKTLILSLQQLFNELVQEQGPNLDRTSAHVSGIKELARRFALTFGLDQIKTREAVATLHKDGIEFAFKYQNQKGQEYPPPNLAFLEVLSEFSSKLLRQDKKTVHSYLEKFLTEQMMERREDVWLPLISYRNSLVTGGEDDRMSVNSGSSSSKTSSVRNKKGRPPLHKKRVEDESLDNTWLNRTDTMIQTPGPLPAPQLTSTVLRENSRPMGDQIQEPESEHGSEPDFLHNPQMQISWLGQPKLEDLNRKDRTGMNYMKVRTGVRHAVRGLMEEDAEPIFEDVMMSSRSQLEDMNEEFEDTMVIDLPPSRNRRERAELRPDFFDSAAIIEDDSGFGMPMF

Target ORF information:

RefSeq Version	NM_005862.2
Organism	Homo sapiens(human)
Definition	Homo sapiens stromal antigen 1 (STAG1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005862.2

ORF Insert Sequence:

ATGATTACTT CAGAATTACC AGTGTTACAG GATTCAACTA ATGAAACTAC TGCCCATTCC 
GATGCTGGCA GCGAGCTTGA AGAAACAGAG GTCAAAGGAA AAAGAAAAAG GGGTCGTCCT 
GGCCGGCCTC CATCTACAAA TAAGAAACCT CGAAAATCTC CAGGTGAGAA GAGCAGAATT 
GAAGCTGGAA TTAGAGGAGC AGGCCGTGGA AGAGCTAATG GACACCCTCA ACAGAATGGG 
GAAGGGGAGC CTGTCACATT ATTTGAGGTG GTGAAACTGG GGAAAAGTGC AATGCAGTCC 
GTGGTGGATG ACTGGATTGA ATCATATAAA CAAGACAGGG ACATCGCACT TCTGGATTTA 
ATCAACTTTT TTATCCAGTG TTCAGGATGT CGAGGTACTG TGAGAATAGA GATGTTTCGA 
AATATGCAGA ATGCAGAAAT CATCAGAAAA ATGACTGAAG AATTTGATGA GGACAGTGGT 
GATTATCCTC TTACCATGCC TGGACCTCAG TGGAAAAAAT TTCGTTCAAA CTTTTGTGAA 
TTTATTGGAG TCCTGATTCG ACAGTGTCAG TATAGCATAA TTTATGATGA GTATATGATG 
GACACAGTAA TCTCCCTTTT GACGGGTTTG TCAGACTCCC AGGTCAGAGC TTTTAGGCAT 
ACAAGTACCC TGGCTGCCAT GAAGCTCATG ACTGCTCTGG TGAATGTTGC CTTAAACCTC 
AGTATTCATC AGGATAATAC CCAGAGACAA TATGAAGCCG AGAGAAATAA AATGATTGGG 
AAGAGAGCCA ATGAAAGGTT GGAGTTACTA CTTCAGAAAC GCAAAGAGCT GCAAGAAAAT 
CAGGATGAAA TCGAAAATAT GATGAACTCT ATTTTTAAGG GTATATTTGT TCATAGATAC 
CGTGATGCTA TTGCTGAGAT TAGAGCCATT TGTATTGAAG AAATTGGAGT ATGGATGAAA 
ATGTATAGTG ATGCCTTCCT AAATGACAGT TACCTAAAAT ATGTTGGCTG GACTCTTCAT 
GACAGGCAAG GGGAAGTCAG GCTGAAGTGT TTGAAAGCTC TGCAGAGTCT ATATACCAAT 
AGAGAATTAT TCCCCAAATT GGAACTATTC ACTAACCGAT TCAAGGATCG CATTGTATCA 
ATGACACTTG ATAAAGAATA TGATGTTGCT GTGGAAGCTA TTCGATTGGT TACTCTGATA 
CTTCATGGAA GTGAAGAAGC TCTTTCCAAT GAAGACTGTG AAAATGTTTA CCACTTGGTG 
TACTCGGCAC ATCGCCCTGT TGCTGTGGCA GCTGGAGAGT TCCTTCACAA AAAGCTATTT 
AGCAGACATG ACCCACAAGC AGAAGAAGCA TTAGCAAAGA GGAGGGGAAG AAACAGCCCG 
AATGGAAACC TCATTAGGAT GCTGGTTCTT TTCTTTCTTG AAAGTGAGTT ACATGAACAT 
GCAGCCTACT TGGTGGACAG TTTATGGGAG AGCTCTCAAG AACTGTTGAA AGACTGGGAA 
TGTATGACAG AGTTGCTATT AGAAGAACCT GTTCAAGGAG AGGAAGCAAT GTCTGATCGT 
CAAGAGAGTG CTCTTATAGA GCTAATGGTT TGTACAATTC GTCAAGCTGC TGAGGCACAT 
CCTCCAGTGG GAAGGGGTAC CGGCAAGAGA GTGCTAACTG CCAAAGAAAG GAAAACTCAA 
ATTGATGATA GAAACAAATT GACTGAACAT TTTATTATTA CACTTCCTAT GTTACTGTCA 
AAGTATTCTG CAGATGCAGA GAAGGTAGCA AACTTGCTAC AAATCCCACA GTATTTTGAT 
TTAGAAATCT ACAGCACAGG TAGAATGGAA AAGCATCTGG ATGCTTTATT AAAACAGATT 
AAGTTTGTTG TGGAGAAACA CGTAGAATCA GATGTTCTAG AAGCCTGCAG TAAAACCTAT 
AGTATCTTAT GCAGTGAAGA ATATACCATC CAGAACAGAG TTGACATAGC TCGAAGCCAG 
CTGATTGATG AGTTTGTAGA TCGATTCAAT CATTCTGTGG AAGACCTATT GCAAGAGGGA 
GAAGAAGCTG ATGATGATGA CATTTACAAT GTTCTTTCTA CATTAAAGCG GTTAACTTCT 
TTTCACAATG CACATGATCT CACAAAATGG GATCTCTTTG GTAATTGCTA CAGATTATTG 
AAGACTGGAA TTGAACATGG AGCCATGCCA GAACAGATAG TCGTGCAAGC ACTGCAGTGT 
TCCCATTATT CGATTCTTTG GCAGTTGGTG AAAATTACTG ATGGCTCTCC TTCCAAAGAG 
GATTTGTTGG TATTGAGGAA AACGGTGAAA TCCTTTTTGG CTGTTTGCCA GCAGTGCCTG 
TCTAATGTTA ATACTCCAGT GAAAGAACAG GCTTTCATGT TACTCTGTGA TCTTCTGATG 
ATTTTCAGCC ACCAATTAAT GACAGGTGGC AGAGAGGGCC TTCAGCCTTT GGTGTTCAAT 
CCAGATACTG GACTCCAATC TGAACTCCTC AGTTTTGTGA TGGATCACGT TTTTATTGAC 
CAAGACGAGG AGAACCAGAG CATGGAGGGT GATGAAGAAG ATGAAGCTAA TAAAATTGAG 
GCCTTACATA AAAGAAGGAA TCTACTTGCT GCTTTCAGCA AACTTATCAT TTATGACATT 
GTTGACATGC ATGCAGCTGC AGACATCTTC AAACACTACA TGAAGTATTA CAATGACTAT 
GGTGATATTA TTAAGGAAAC ACTGAGTAAA ACCAGGCAGA TTGATAAAAT TCAGTGTGCC 
AAGACTCTCA TTCTCAGTTT GCAACAGTTA TTTAATGAAC TTGTTCAAGA GCAAGGTCCC 
AACCTAGATA GGACATCTGC CCATGTCAGT GGCATTAAAG AACTGGCACG TCGCTTTGCC 
CTTACATTTG GATTGGACCA GATTAAGACA CGAGAAGCAG TTGCCACACT TCACAAGGAT 
GGCATAGAGT TTGCATTTAA ATACCAAAAT CAGAAAGGAC AAGAGTATCC ACCTCCTAAT 
CTGGCTTTTC TTGAAGTACT AAGTGAATTT TCTTCTAAAC TTCTTCGACA GGACAAAAAG 
ACAGTTCATT CATACCTAGA GAAATTCCTT ACCGAGCAGA TGATGGAAAG GAGGGAGGAT 
GTATGGCTTC CACTCATCTC CTATAGAAAT TCATTAGTCA CTGGGGGTGA AGATGATAGA 
ATGTCTGTGA ACAGTGGAAG TAGCAGCAGC AAAACCTCAT CAGTAAGGAA TAAGAAAGGA 
CGACCTCCAC TTCATAAAAA ACGAGTAGAA GATGAGAGTC TGGATAACAC ATGGCTAAAC 
AGGACTGACA CCATGATTCA GACTCCTGGC CCCCTGCCAG CACCACAACT CACATCCACT 
GTACTGCGGG AGAACAGTCG GCCCATGGGA GACCAGATTC AAGAACCTGA GTCTGAACAT 
GGTTCTGAAC CAGACTTTTT ACACAATCCT CAGATGCAGA TCTCTTGGTT AGGCCAGCCG 
AAGTTAGAAG ACTTAAATCG GAAGGACAGA ACAGGAATGA ACTACATGAA AGTGAGAACT 
GGAGTGAGGC ATGCTGTTCG GGGTCTAATG GAGGAAGATG CTGAGCCCAT CTTTGAAGAT 
GTGATGATGT CATCCCGAAG CCAGTTAGAA GATATGAATG AAGAATTTGA GGACACCATG 
GTTATTGATC TGCCTCCATC AAGAAATCGG CGAGAGAGAG CTGAGCTAAG GCCAGACTTC 
TTTGACTCTG CAGCTATCAT AGAAGATGAT TCAGGATTTG GAATGCCTAT GTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

STAG1 ( NM_005862.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_005853.2 Homo sapiens stromal antigen 1 (STAG1), mRNA.

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CloneID	OHu15240
Clone ID Related Accession (Same CDS sequence)	NM_005862.2 , NM_005862.3
Accession Version	NM_005862.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3777bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-25
Organism	Homo sapiens(human)
Product	cohesin subunit SA-1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC064699.1, BC017735.1 and AC069524.18. On Nov 23, 2018 this sequence version replaced NM_005862.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.141831.1, SRR1803612.57129.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000383202.7/ ENSP00000372689.2 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGATTACTT CAGAATTACC AGTGTTACAG GATTCAACTA ATGAAACTAC TGCCCATTCC 
GATGCTGGCA GCGAGCTTGA AGAAACAGAG GTCAAAGGAA AAAGAAAAAG GGGTCGTCCT 
GGCCGGCCTC CATCTACAAA TAAGAAACCT CGAAAATCTC CAGGTGAGAA GAGCAGAATT 
GAAGCTGGAA TTAGAGGAGC AGGCCGTGGA AGAGCTAATG GACACCCTCA ACAGAATGGG 
GAAGGGGAGC CTGTCACATT ATTTGAGGTG GTGAAACTGG GGAAAAGTGC AATGCAGTCC 
GTGGTGGATG ACTGGATTGA ATCATATAAA CAAGACAGGG ACATCGCACT TCTGGATTTA 
ATCAACTTTT TTATCCAGTG TTCAGGATGT CGAGGTACTG TGAGAATAGA GATGTTTCGA 
AATATGCAGA ATGCAGAAAT CATCAGAAAA ATGACTGAAG AATTTGATGA GGACAGTGGT 
GATTATCCTC TTACCATGCC TGGACCTCAG TGGAAAAAAT TTCGTTCAAA CTTTTGTGAA 
TTTATTGGAG TCCTGATTCG ACAGTGTCAG TATAGCATAA TTTATGATGA GTATATGATG 
GACACAGTAA TCTCCCTTTT GACGGGTTTG TCAGACTCCC AGGTCAGAGC TTTTAGGCAT 
ACAAGTACCC TGGCTGCCAT GAAGCTCATG ACTGCTCTGG TGAATGTTGC CTTAAACCTC 
AGTATTCATC AGGATAATAC CCAGAGACAA TATGAAGCCG AGAGAAATAA AATGATTGGG 
AAGAGAGCCA ATGAAAGGTT GGAGTTACTA CTTCAGAAAC GCAAAGAGCT GCAAGAAAAT 
CAGGATGAAA TCGAAAATAT GATGAACTCT ATTTTTAAGG GTATATTTGT TCATAGATAC 
CGTGATGCTA TTGCTGAGAT TAGAGCCATT TGTATTGAAG AAATTGGAGT ATGGATGAAA 
ATGTATAGTG ATGCCTTCCT AAATGACAGT TACCTAAAAT ATGTTGGCTG GACTCTTCAT 
GACAGGCAAG GGGAAGTCAG GCTGAAGTGT TTGAAAGCTC TGCAGAGTCT ATATACCAAT 
AGAGAATTAT TCCCCAAATT GGAACTATTC ACTAACCGAT TCAAGGATCG CATTGTATCA 
ATGACACTTG ATAAAGAATA TGATGTTGCT GTGGAAGCTA TTCGATTGGT TACTCTGATA 
CTTCATGGAA GTGAAGAAGC TCTTTCCAAT GAAGACTGTG AAAATGTTTA CCACTTGGTG 
TACTCGGCAC ATCGCCCTGT TGCTGTGGCA GCTGGAGAGT TCCTTCACAA AAAGCTATTT 
AGCAGACATG ACCCACAAGC AGAAGAAGCA TTAGCAAAGA GGAGGGGAAG AAACAGCCCG 
AATGGAAACC TCATTAGGAT GCTGGTTCTT TTCTTTCTTG AAAGTGAGTT ACATGAACAT 
GCAGCCTACT TGGTGGACAG TTTATGGGAG AGCTCTCAAG AACTGTTGAA AGACTGGGAA 
TGTATGACAG AGTTGCTATT AGAAGAACCT GTTCAAGGAG AGGAAGCAAT GTCTGATCGT 
CAAGAGAGTG CTCTTATAGA GCTAATGGTT TGTACAATTC GTCAAGCTGC TGAGGCACAT 
CCTCCAGTGG GAAGGGGTAC CGGCAAGAGA GTGCTAACTG CCAAAGAAAG GAAAACTCAA 
ATTGATGATA GAAACAAATT GACTGAACAT TTTATTATTA CACTTCCTAT GTTACTGTCA 
AAGTATTCTG CAGATGCAGA GAAGGTAGCA AACTTGCTAC AAATCCCACA GTATTTTGAT 
TTAGAAATCT ACAGCACAGG TAGAATGGAA AAGCATCTGG ATGCTTTATT AAAACAGATT 
AAGTTTGTTG TGGAGAAACA CGTAGAATCA GATGTTCTAG AAGCCTGCAG TAAAACCTAT 
AGTATCTTAT GCAGTGAAGA ATATACCATC CAGAACAGAG TTGACATAGC TCGAAGCCAG 
CTGATTGATG AGTTTGTAGA TCGATTCAAT CATTCTGTGG AAGACCTATT GCAAGAGGGA 
GAAGAAGCTG ATGATGATGA CATTTACAAT GTTCTTTCTA CATTAAAGCG GTTAACTTCT 
TTTCACAATG CACATGATCT CACAAAATGG GATCTCTTTG GTAATTGCTA CAGATTATTG 
AAGACTGGAA TTGAACATGG AGCCATGCCA GAACAGATAG TCGTGCAAGC ACTGCAGTGT 
TCCCATTATT CGATTCTTTG GCAGTTGGTG AAAATTACTG ATGGCTCTCC TTCCAAAGAG 
GATTTGTTGG TATTGAGGAA AACGGTGAAA TCCTTTTTGG CTGTTTGCCA GCAGTGCCTG 
TCTAATGTTA ATACTCCAGT GAAAGAACAG GCTTTCATGT TACTCTGTGA TCTTCTGATG 
ATTTTCAGCC ACCAATTAAT GACAGGTGGC AGAGAGGGCC TTCAGCCTTT GGTGTTCAAT 
CCAGATACTG GACTCCAATC TGAACTCCTC AGTTTTGTGA TGGATCACGT TTTTATTGAC 
CAAGACGAGG AGAACCAGAG CATGGAGGGT GATGAAGAAG ATGAAGCTAA TAAAATTGAG 
GCCTTACATA AAAGAAGGAA TCTACTTGCT GCTTTCAGCA AACTTATCAT TTATGACATT 
GTTGACATGC ATGCAGCTGC AGACATCTTC AAACACTACA TGAAGTATTA CAATGACTAT 
GGTGATATTA TTAAGGAAAC ACTGAGTAAA ACCAGGCAGA TTGATAAAAT TCAGTGTGCC 
AAGACTCTCA TTCTCAGTTT GCAACAGTTA TTTAATGAAC TTGTTCAAGA GCAAGGTCCC 
AACCTAGATA GGACATCTGC CCATGTCAGT GGCATTAAAG AACTGGCACG TCGCTTTGCC 
CTTACATTTG GATTGGACCA GATTAAGACA CGAGAAGCAG TTGCCACACT TCACAAGGAT 
GGCATAGAGT TTGCATTTAA ATACCAAAAT CAGAAAGGAC AAGAGTATCC ACCTCCTAAT 
CTGGCTTTTC TTGAAGTACT AAGTGAATTT TCTTCTAAAC TTCTTCGACA GGACAAAAAG 
ACAGTTCATT CATACCTAGA GAAATTCCTT ACCGAGCAGA TGATGGAAAG GAGGGAGGAT 
GTATGGCTTC CACTCATCTC CTATAGAAAT TCATTAGTCA CTGGGGGTGA AGATGATAGA 
ATGTCTGTGA ACAGTGGAAG TAGCAGCAGC AAAACCTCAT CAGTAAGGAA TAAGAAAGGA 
CGACCTCCAC TTCATAAAAA ACGAGTAGAA GATGAGAGTC TGGATAACAC ATGGCTAAAC 
AGGACTGACA CCATGATTCA GACTCCTGGC CCCCTGCCAG CACCACAACT CACATCCACT 
GTACTGCGGG AGAACAGTCG GCCCATGGGA GACCAGATTC AAGAACCTGA GTCTGAACAT 
GGTTCTGAAC CAGACTTTTT ACACAATCCT CAGATGCAGA TCTCTTGGTT AGGCCAGCCG 
AAGTTAGAAG ACTTAAATCG GAAGGACAGA ACAGGAATGA ACTACATGAA AGTGAGAACT 
GGAGTGAGGC ATGCTGTTCG GGGTCTAATG GAGGAAGATG CTGAGCCCAT CTTTGAAGAT 
GTGATGATGT CATCCCGAAG CCAGTTAGAA GATATGAATG AAGAATTTGA GGACACCATG 
GTTATTGATC TGCCTCCATC AAGAAATCGG CGAGAGAGAG CTGAGCTAAG GCCAGACTTC 
TTTGACTCTG CAGCTATCAT AGAAGATGAT TCAGGATTTG GAATGCCTAT GTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005853.2
CDS	268..4044
Translation	MITSELPVLQDSTNETTAHSDAGSELEETEVKGKRKRGRPGRPPSTNKKPRKSPGEKSRIEAGIRGAGRGRANGHPQQNGEGEPVTLFEVVKLGKSAMQSVVDDWIESYKQDRDIALLDLINFFIQCSGCRGTVRIEMFRNMQNAEIIRKMTEEFDEDSGDYPLTMPGPQWKKFRSNFCEFIGVLIRQCQYSIIYDEYMMDTVISLLTGLSDSQVRAFRHTSTLAAMKLMTALVNVALNLSIHQDNTQRQYEAERNKMIGKRANERLELLLQKRKELQENQDEIENMMNSIFKGIFVHRYRDAIAEIRAICIEEIGVWMKMYSDAFLNDSYLKYVGWTLHDRQGEVRLKCLKALQSLYTNRELFPKLELFTNRFKDRIVSMTLDKEYDVAVEAIRLVTLILHGSEEALSNEDCENVYHLVYSAHRPVAVAAGEFLHKKLFSRHDPQAEEALAKRRGRNSPNGNLIRMLVLFFLESELHEHAAYLVDSLWESSQELLKDWECMTELLLEEPVQGEEAMSDRQESALIELMVCTIRQAAEAHPPVGRGTGKRVLTAKERKTQIDDRNKLTEHFIITLPMLLSKYSADAEKVANLLQIPQYFDLEIYSTGRMEKHLDALLKQIKFVVEKHVESDVLEACSKTYSILCSEEYTIQNRVDIARSQLIDEFVDRFNHSVEDLLQEGEEADDDDIYNVLSTLKRLTSFHNAHDLTKWDLFGNCYRLLKTGIEHGAMPEQIVVQALQCSHYSILWQLVKITDGSPSKEDLLVLRKTVKSFLAVCQQCLSNVNTPVKEQAFMLLCDLLMIFSHQLMTGGREGLQPLVFNPDTGLQSELLSFVMDHVFIDQDEENQSMEGDEEDEANKIEALHKRRNLLAAFSKLIIYDIVDMHAAADIFKHYMKYYNDYGDIIKETLSKTRQIDKIQCAKTLILSLQQLFNELVQEQGPNLDRTSAHVSGIKELARRFALTFGLDQIKTREAVATLHKDGIEFAFKYQNQKGQEYPPPNLAFLEVLSEFSSKLLRQDKKTVHSYLEKFLTEQMMERREDVWLPLISYRNSLVTGGEDDRMSVNSGSSSSKTSSVRNKKGRPPLHKKRVEDESLDNTWLNRTDTMIQTPGPLPAPQLTSTVLRENSRPMGDQIQEPESEHGSEPDFLHNPQMQISWLGQPKLEDLNRKDRTGMNYMKVRTGVRHAVRGLMEEDAEPIFEDVMMSSRSQLEDMNEEFEDTMVIDLPPSRNRRERAELRPDFFDSAAIIEDDSGFGMPMF

Target ORF information:

RefSeq Version	NM_005862.3
Organism	Homo sapiens(human)
Definition	Homo sapiens stromal antigen 1 (STAG1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005862.3

ORF Insert Sequence:

ATGATTACTT CAGAATTACC AGTGTTACAG GATTCAACTA ATGAAACTAC TGCCCATTCC 
GATGCTGGCA GCGAGCTTGA AGAAACAGAG GTCAAAGGAA AAAGAAAAAG GGGTCGTCCT 
GGCCGGCCTC CATCTACAAA TAAGAAACCT CGAAAATCTC CAGGTGAGAA GAGCAGAATT 
GAAGCTGGAA TTAGAGGAGC AGGCCGTGGA AGAGCTAATG GACACCCTCA ACAGAATGGG 
GAAGGGGAGC CTGTCACATT ATTTGAGGTG GTGAAACTGG GGAAAAGTGC AATGCAGTCC 
GTGGTGGATG ACTGGATTGA ATCATATAAA CAAGACAGGG ACATCGCACT TCTGGATTTA 
ATCAACTTTT TTATCCAGTG TTCAGGATGT CGAGGTACTG TGAGAATAGA GATGTTTCGA 
AATATGCAGA ATGCAGAAAT CATCAGAAAA ATGACTGAAG AATTTGATGA GGACAGTGGT 
GATTATCCTC TTACCATGCC TGGACCTCAG TGGAAAAAAT TTCGTTCAAA CTTTTGTGAA 
TTTATTGGAG TCCTGATTCG ACAGTGTCAG TATAGCATAA TTTATGATGA GTATATGATG 
GACACAGTAA TCTCCCTTTT GACGGGTTTG TCAGACTCCC AGGTCAGAGC TTTTAGGCAT 
ACAAGTACCC TGGCTGCCAT GAAGCTCATG ACTGCTCTGG TGAATGTTGC CTTAAACCTC 
AGTATTCATC AGGATAATAC CCAGAGACAA TATGAAGCCG AGAGAAATAA AATGATTGGG 
AAGAGAGCCA ATGAAAGGTT GGAGTTACTA CTTCAGAAAC GCAAAGAGCT GCAAGAAAAT 
CAGGATGAAA TCGAAAATAT GATGAACTCT ATTTTTAAGG GTATATTTGT TCATAGATAC 
CGTGATGCTA TTGCTGAGAT TAGAGCCATT TGTATTGAAG AAATTGGAGT ATGGATGAAA 
ATGTATAGTG ATGCCTTCCT AAATGACAGT TACCTAAAAT ATGTTGGCTG GACTCTTCAT 
GACAGGCAAG GGGAAGTCAG GCTGAAGTGT TTGAAAGCTC TGCAGAGTCT ATATACCAAT 
AGAGAATTAT TCCCCAAATT GGAACTATTC ACTAACCGAT TCAAGGATCG CATTGTATCA 
ATGACACTTG ATAAAGAATA TGATGTTGCT GTGGAAGCTA TTCGATTGGT TACTCTGATA 
CTTCATGGAA GTGAAGAAGC TCTTTCCAAT GAAGACTGTG AAAATGTTTA CCACTTGGTG 
TACTCGGCAC ATCGCCCTGT TGCTGTGGCA GCTGGAGAGT TCCTTCACAA AAAGCTATTT 
AGCAGACATG ACCCACAAGC AGAAGAAGCA TTAGCAAAGA GGAGGGGAAG AAACAGCCCG 
AATGGAAACC TCATTAGGAT GCTGGTTCTT TTCTTTCTTG AAAGTGAGTT ACATGAACAT 
GCAGCCTACT TGGTGGACAG TTTATGGGAG AGCTCTCAAG AACTGTTGAA AGACTGGGAA 
TGTATGACAG AGTTGCTATT AGAAGAACCT GTTCAAGGAG AGGAAGCAAT GTCTGATCGT 
CAAGAGAGTG CTCTTATAGA GCTAATGGTT TGTACAATTC GTCAAGCTGC TGAGGCACAT 
CCTCCAGTGG GAAGGGGTAC CGGCAAGAGA GTGCTAACTG CCAAAGAAAG GAAAACTCAA 
ATTGATGATA GAAACAAATT GACTGAACAT TTTATTATTA CACTTCCTAT GTTACTGTCA 
AAGTATTCTG CAGATGCAGA GAAGGTAGCA AACTTGCTAC AAATCCCACA GTATTTTGAT 
TTAGAAATCT ACAGCACAGG TAGAATGGAA AAGCATCTGG ATGCTTTATT AAAACAGATT 
AAGTTTGTTG TGGAGAAACA CGTAGAATCA GATGTTCTAG AAGCCTGCAG TAAAACCTAT 
AGTATCTTAT GCAGTGAAGA ATATACCATC CAGAACAGAG TTGACATAGC TCGAAGCCAG 
CTGATTGATG AGTTTGTAGA TCGATTCAAT CATTCTGTGG AAGACCTATT GCAAGAGGGA 
GAAGAAGCTG ATGATGATGA CATTTACAAT GTTCTTTCTA CATTAAAGCG GTTAACTTCT 
TTTCACAATG CACATGATCT CACAAAATGG GATCTCTTTG GTAATTGCTA CAGATTATTG 
AAGACTGGAA TTGAACATGG AGCCATGCCA GAACAGATAG TCGTGCAAGC ACTGCAGTGT 
TCCCATTATT CGATTCTTTG GCAGTTGGTG AAAATTACTG ATGGCTCTCC TTCCAAAGAG 
GATTTGTTGG TATTGAGGAA AACGGTGAAA TCCTTTTTGG CTGTTTGCCA GCAGTGCCTG 
TCTAATGTTA ATACTCCAGT GAAAGAACAG GCTTTCATGT TACTCTGTGA TCTTCTGATG 
ATTTTCAGCC ACCAATTAAT GACAGGTGGC AGAGAGGGCC TTCAGCCTTT GGTGTTCAAT 
CCAGATACTG GACTCCAATC TGAACTCCTC AGTTTTGTGA TGGATCACGT TTTTATTGAC 
CAAGACGAGG AGAACCAGAG CATGGAGGGT GATGAAGAAG ATGAAGCTAA TAAAATTGAG 
GCCTTACATA AAAGAAGGAA TCTACTTGCT GCTTTCAGCA AACTTATCAT TTATGACATT 
GTTGACATGC ATGCAGCTGC AGACATCTTC AAACACTACA TGAAGTATTA CAATGACTAT 
GGTGATATTA TTAAGGAAAC ACTGAGTAAA ACCAGGCAGA TTGATAAAAT TCAGTGTGCC 
AAGACTCTCA TTCTCAGTTT GCAACAGTTA TTTAATGAAC TTGTTCAAGA GCAAGGTCCC 
AACCTAGATA GGACATCTGC CCATGTCAGT GGCATTAAAG AACTGGCACG TCGCTTTGCC 
CTTACATTTG GATTGGACCA GATTAAGACA CGAGAAGCAG TTGCCACACT TCACAAGGAT 
GGCATAGAGT TTGCATTTAA ATACCAAAAT CAGAAAGGAC AAGAGTATCC ACCTCCTAAT 
CTGGCTTTTC TTGAAGTACT AAGTGAATTT TCTTCTAAAC TTCTTCGACA GGACAAAAAG 
ACAGTTCATT CATACCTAGA GAAATTCCTT ACCGAGCAGA TGATGGAAAG GAGGGAGGAT 
GTATGGCTTC CACTCATCTC CTATAGAAAT TCATTAGTCA CTGGGGGTGA AGATGATAGA 
ATGTCTGTGA ACAGTGGAAG TAGCAGCAGC AAAACCTCAT CAGTAAGGAA TAAGAAAGGA 
CGACCTCCAC TTCATAAAAA ACGAGTAGAA GATGAGAGTC TGGATAACAC ATGGCTAAAC 
AGGACTGACA CCATGATTCA GACTCCTGGC CCCCTGCCAG CACCACAACT CACATCCACT 
GTACTGCGGG AGAACAGTCG GCCCATGGGA GACCAGATTC AAGAACCTGA GTCTGAACAT 
GGTTCTGAAC CAGACTTTTT ACACAATCCT CAGATGCAGA TCTCTTGGTT AGGCCAGCCG 
AAGTTAGAAG ACTTAAATCG GAAGGACAGA ACAGGAATGA ACTACATGAA AGTGAGAACT 
GGAGTGAGGC ATGCTGTTCG GGGTCTAATG GAGGAAGATG CTGAGCCCAT CTTTGAAGAT 
GTGATGATGT CATCCCGAAG CCAGTTAGAA GATATGAATG AAGAATTTGA GGACACCATG 
GTTATTGATC TGCCTCCATC AAGAAATCGG CGAGAGAGAG CTGAGCTAAG GCCAGACTTC 
TTTGACTCTG CAGCTATCAT AGAAGATGAT TCAGGATTTG GAATGCCTAT GTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Synthetic lethality between the cohesin subunits
eLife6(2017 Jul)
van der Lelij P,Lieb S,Jude J,Wutz G,Santos CP,Falkenberg K,Schlattl A,Ban J,Schwentner R,Hoffmann T,Kovar H,Real FX,Waldman T,Pearson MA,Kraut N,Peters JM,Zuber J,Petronczki M

Synthetic lethal interaction between the tumour suppressor STAG2 and its paralog STAG1.
Oncotarget8(23)37619-37632(2017 Jun)
Benedetti L,Cereda M,Monteverde L,Desai N,Ciccarelli FD

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STAG1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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