CDKN1B cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	CDKN1B
Entrez Gene ID	1027
Full Name	cyclin dependent kinase inhibitor 1B
Synonyms	CDKN4,KIP1,MEN1B,MEN4,P27KIP1
General protein information	Preferred Names cyclin dependent kinase inhibitor 1B Names cyclin-dependent kinase inhibitor 1B cyclin-dependent kinase inhibitor 1B (p27, Kip1)
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 12 Map Location: 12p13.1
Summary	This gene encodes a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. Mutations in this gene are associated with multiple endocrine neoplasia type IV (MEN4). [provided by RefSeq, Apr 2014].
Disorder MIM:	600778
Disorder Html:	Multiple endocrine neoplasia, type IV, 610755 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_004064.4	NP_004055.1	cyclin-dependent kinase inhibitor 1B

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	CDKN1B	XP_522347.3
Canis lupus familiaris (dog)	CDKN1B	NP_001002957.1
Bos taurus (cattle)	CDKN1B	NP_001093816.1
	CDKN1B	XP_001085433.1
Danio rerio (zebrafish)	cdkn1bb	NP_997957.1
Danio rerio (zebrafish)	LOC100534692	XP_003201420.1
Rattus norvegicus (Norway rat)	Cdkn1b	NP_113950.2
Homo sapiens (human)	CDKN1B	NP_004055.1
Mus musculus (house mouse)	Cdkn1b	NP_034005.2
Gallus gallus (chicken)	CDKN1B	NP_989587.1

Gene Ontology
Bibliography

Related articles in PubMed

A novel mutation in the upstream open reading frame of the CDKN1B gene causes a MEN4 phenotype.
Occhi G, Regazzo D, Trivellin G, Boaretto F, Ciato D, Bobisse S, Ferasin S, Cetani F, Pardi E, Korbonits M, Pellegata NS, Sidarovich V, Quattrone A, Opocher G, Mantero F, Scaroni C
PLoS genetics9(3)e1003350(2013 Mar)

Germ-line mutations in p27Kip1 cause a multiple endocrine neoplasia syndrome in rats and humans.
Pellegata NS, Quintanilla-Martinez L, Siggelkow H, Samson E, Bink K, H?fler H, Fend F, Graw J, Atkinson MJ
Proceedings of the National Academy of Sciences of the United States of America103(42)15558-63(2006 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

PTEN loss and p27 loss differ among morphologic patterns of prostate cancer.
Title: PTEN loss and p27 loss differ among morphologic patterns of prostate cancer, including cribriform.

abnormal levels of Skp2 and p27(KIP1) have probably been involved in the pathogenesis of ADH and DCIS. Thus, Skp2 and p27(KIP1) may serve as important diagnosis markers.
Title: Aberrant Level of Skp2 and p27KIP1 in Intraductal Proliferative Lesions is Associated with Tumorigenesis.

Data show that the resveratrol/docetaxel combination up regulated the expression of tumor supressor p53 (p53), and cell cycle inhibitors p21WAF1/CIP1 and p27KIP1.
Title: Resveratrol induces cell cycle arrest and apoptosis with docetaxel in prostate cancer cells via a p53/ p21WAF1/CIP1 and p27KIP1 pathway.

SNHG6 acted as an oncogene in gastric cancer cells through regulating miR-101-3p/ZEB1 at a post-transcriptional level and silencing expression at a transcriptional level by recruiting enhancer of zeste homolog 2 (EZH2) to the promoter of p27.
Title: LncRNA SNHG6 is Associated with Poor Prognosis of Gastric Cancer and Promotes Cell Proliferation and EMT through Epigenetically Silencing p27 and Sponging miR-101-3p.

Our study was the first to screen for CDKN1B mutations, which have been shown to cause MEN4, in a Chinese population. Nevertheless, no CDKN1B mutation was detected in the present study, which supports the rarity of CDKN1B gene mutation.
Title: Clinical and Genetic Analysis of Multiple Endocrine Neoplasia Type 1-Related Primary Hyperparathyroidism in Chinese.

The following CDKN1B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CDKN1B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHu27895	NM_004064.4 Latest version!	Homo sapiens cyclin dependent kinase inhibitor 1B (CDKN1B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CDKN1B ( NM_004064.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_004055.1 Homo sapiens cyclin dependent kinase inhibitor 1B (CDKN1B), mRNA.

Price & Availability↑

CloneID	OHu27895
Clone ID Related Accession (Same CDS sequence)	NM_004064.4
Accession Version	NM_004064.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 597bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Homo sapiens(human)
Product	cyclin-dependent kinase inhibitor 1B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY097069.1, AK312461.1, BC001971.1, AA833962.1 and HY246392.1. This sequence is a reference standard in the RefSeqGene project. On Feb 12, 2014 this sequence version replaced NM_004064.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.100339.1, SRR1803612.6849.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on single protein-coding transcript regulatory uORF :: PMID: 12837699 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTCAAACG TGCGAGTGTC TAACGGGAGC CCTAGCCTGG AGCGGATGGA CGCCAGGCAG 
GCGGAGCACC CCAAGCCCTC GGCCTGCAGG AACCTCTTCG GCCCGGTGGA CCACGAAGAG 
TTAACCCGGG ACTTGGAGAA GCACTGCAGA GACATGGAAG AGGCGAGCCA GCGCAAGTGG 
AATTTCGATT TTCAGAATCA CAAACCCCTA GAGGGCAAGT ACGAGTGGCA AGAGGTGGAG 
AAGGGCAGCT TGCCCGAGTT CTACTACAGA CCCCCGCGGC CCCCCAAAGG TGCCTGCAAG 
GTGCCGGCGC AGGAGAGCCA GGATGTCAGC GGGAGCCGCC CGGCGGCGCC TTTAATTGGG 
GCTCCGGCTA ACTCTGAGGA CACGCATTTG GTGGACCCAA AGACTGATCC GTCGGACAGC 
CAGACGGGGT TAGCGGAGCA ATGCGCAGGA ATAAGGAAGC GACCTGCAAC CGACGATTCT 
TCTACTCAAA ACAAAAGAGC CAACAGAACA GAAGAAAATG TTTCAGACGG TTCCCCAAAT 
GCCGGTTCTG TGGAGCAGAC GCCCAAGAAG CCTGGCCTCA GAAGACGTCA AACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004055.1
CDS	571..1167
Translation	MSNVRVSNGSPSLERMDARQAEHPKPSACRNLFGPVDHEELTRDLEKHCRDMEEASQRKWNFDFQNHKPLEGKYEWQEVEKGSLPEFYYRPPRPPKGACKVPAQESQDVSGSRPAAPLIGAPANSEDTHLVDPKTDPSDSQTGLAEQCAGIRKRPATDDSSTQNKRANRTEENVSDGSPNAGSVEQTPKKPGLRRRQT

Target ORF information:

RefSeq Version	NM_004064.4
Organism	Homo sapiens(human)
Definition	Homo sapiens cyclin dependent kinase inhibitor 1B (CDKN1B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004064.4

ORF Insert Sequence:

ATGTCAAACG TGCGAGTGTC TAACGGGAGC CCTAGCCTGG AGCGGATGGA CGCCAGGCAG 
GCGGAGCACC CCAAGCCCTC GGCCTGCAGG AACCTCTTCG GCCCGGTGGA CCACGAAGAG 
TTAACCCGGG ACTTGGAGAA GCACTGCAGA GACATGGAAG AGGCGAGCCA GCGCAAGTGG 
AATTTCGATT TTCAGAATCA CAAACCCCTA GAGGGCAAGT ACGAGTGGCA AGAGGTGGAG 
AAGGGCAGCT TGCCCGAGTT CTACTACAGA CCCCCGCGGC CCCCCAAAGG TGCCTGCAAG 
GTGCCGGCGC AGGAGAGCCA GGATGTCAGC GGGAGCCGCC CGGCGGCGCC TTTAATTGGG 
GCTCCGGCTA ACTCTGAGGA CACGCATTTG GTGGACCCAA AGACTGATCC GTCGGACAGC 
CAGACGGGGT TAGCGGAGCA ATGCGCAGGA ATAAGGAAGC GACCTGCAAC CGACGATTCT 
TCTACTCAAA ACAAAAGAGC CAACAGAACA GAAGAAAATG TTTCAGACGG TTCCCCAAAT 
GCCGGTTCTG TGGAGCAGAC GCCCAAGAAG CCTGGCCTCA GAAGACGTCA AACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A novel mutation in the upstream open reading frame of the CDKN1B gene causes a MEN4 phenotype.
PLoS genetics9(3)e1003350(2013 Mar)
Occhi G,Regazzo D,Trivellin G,Boaretto F,Ciato D,Bobisse S,Ferasin S,Cetani F,Pardi E,Korbonits M,Pellegata NS,Sidarovich V,Quattrone A,Opocher G,Mantero F,Scaroni C

Germ-line mutations in p27Kip1 cause a multiple endocrine neoplasia syndrome in rats and humans.
Proceedings of the National Academy of Sciences of the United States of America103(42)15558-63(2006 Oct)
Pellegata NS,Quintanilla-Martinez L,Siggelkow H,Samson E,Bink K,H?fler H,Fend F,Graw J,Atkinson MJ

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CDKN1B cDNA ORF clone, Homo sapiens(human)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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