CNKSR1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	CNKSR1
Entrez Gene ID	10256
Full Name	connector enhancer of kinase suppressor of Ras 1
Synonyms	CNK,CNK1
General protein information	Preferred Names connector enhancer of kinase suppressor of Ras 1 Names connector enhancer of kinase suppressor of ras 1 CNK homolog protein 1 connector enhancer of KSR 1 connector enhancer of KSR-like
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1p36.11
Summary	This gene encodes a protein containing several motifs involved in protein-protein interaction, including PDZ, PH (Pleckstrin homology), and SAM (sterile alpha motif) domains. The encoded protein acts as a scaffold component for receptor tyrosine kinase signaling and may mediate crosstalk between different signaling pathways. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014].
Disorder MIM:	603272

mRNA and Protein(s)

mRNA	Protein	Name
NM_001297647.1	NP_001284576.1	connector enhancer of kinase suppressor of ras 1 isoform 2
NM_001297647.2	NP_001284576.1	connector enhancer of kinase suppressor of ras 1 isoform 2
NM_006314.3	NP_006305.2	connector enhancer of kinase suppressor of ras 1 isoform 1
XM_011540485.2	XP_011538787.1	connector enhancer of kinase suppressor of ras 1 isoform X2
XM_011540484.2	XP_011538786.1	connector enhancer of kinase suppressor of ras 1 isoform X1
NM_006314.2	NP_006305.2	connector enhancer of kinase suppressor of ras 1 isoform 1
NM_001297648.1	NP_001284577.1	connector enhancer of kinase suppressor of ras 1 isoform 3
NM_001297648.2	NP_001284577.1	connector enhancer of kinase suppressor of ras 1 isoform 3

Pathways from BioSystems

Homologies

	CNKSR1	XP_002808325.1
Xenopus tropicalis (tropical clawed frog)	cnksr1	NP_001116896.1
Rattus norvegicus (Norway rat)	Cnksr1	NP_001034100.1
Gallus gallus (chicken)	CNKSR1	XP_004947732.1
Homo sapiens (human)	CNKSR1	NP_006305.2
Canis lupus familiaris (dog)	CNKSR1	XP_003638970.2
Danio rerio (zebrafish)	cnksr1	NP_991222.1
Pan troglodytes (chimpanzee)	CNKSR1	XP_524626.2
Bos taurus (cattle)	LOC617552	NP_001180094.1
Mus musculus (house mouse)	Cnksr1	NP_001074516.1

Gene Ontology
Bibliography

Related articles in PubMed

Expression of the scaffold connector enhancer of kinase suppressor of Ras 1 (CNKSR1) is correlated with clinical outcome in pancreatic cancer.
Quadri HS, Aiken TJ, Allgaeuer M, Moravec R, Altekruse S, Hussain SP, Miettinen MM, Hewitt SM, Rudloff U
BMC cancer17(1)495(2017 Jul)

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

AKT-dependent phosphorylation of the SAM domain induces oligomerization and activation of the scaffold protein CNK1.
Fischer A, Weber W, Warscheid B, Radziwill G
Biochimica et biophysica acta1864(1)89-100(2017 01)

Optogenetic clustering of CNK1 reveals mechanistic insights in RAF and AKT signalling controlling cell fate decisions.
Fischer A, Warscheid B, Weber W, Radziwill G
Scientific reports638155(2016 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The findings reveal SAM domain-dependent oligomerization by AKT as a switch for CNK1 activation.
Title: AKT-dependent phosphorylation of the SAM domain induces oligomerization and activation of the scaffold protein CNK1.

tyrosine phosphorylation is an important mechanism to control the subcellular localization of CNK1 and its distinct biological functions.
Title: Differential tyrosine phosphorylation controls the function of CNK1 as a molecular switch in signal transduction.

CNK1 mediates ephrinB1 signaling that promotes cell migration through RhoA and JNK activity.
Title: EphrinB1 interacts with CNK1 and promotes cell migration through c-Jun N-terminal kinase (JNK) activation.

Studies indicate that CNK1-driven proliferation relies on Akt-dependent phosphorylation and inactivation of FoxO proteins.
Title: CNK1 and other scaffolds for Akt/FoxO signaling.

These findings identify CNK1 as a new positive regulator of insulin signaling.
Title: The CNK1 scaffold binds cytohesins and promotes insulin pathway signaling.

The following CNKSR1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CNKSR1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu32910	NM_001297647.2 Latest version!	Homo sapiens connector enhancer of kinase suppressor of Ras 1 (CNKSR1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00
OHu32911	NM_001297648.2 Latest version!	Homo sapiens connector enhancer of kinase suppressor of Ras 1 (CNKSR1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
OHu32911	XM_011540484.2 Latest version!	Homo sapiens connector enhancer of kinase suppressor of Ras 1 (CNKSR1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
OHu63818	XM_011540485.2 Latest version!	Homo sapiens connector enhancer of kinase suppressor of Ras 1 (CNKSR1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
OHu31462	NM_006314.3 Latest version!	Homo sapiens connector enhancer of kinase suppressor of Ras 1 (CNKSR1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$503.30 $719.00
View Old Accession Versions >>
OHu32910	NM_001297647.1	Homo sapiens connector enhancer of kinase suppressor of Ras 1 (CNKSR1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00
OHu31462	NM_006314.2	Homo sapiens connector enhancer of kinase suppressor of Ras 1 (CNKSR1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$503.30 $719.00
OHu32911	NM_001297648.1	Homo sapiens connector enhancer of kinase suppressor of Ras 1 (CNKSR1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CNKSR1 ( NM_001297647.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001284576.1 Homo sapiens connector enhancer of kinase suppressor of Ras 1 (CNKSR1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu32910
Clone ID Related Accession (Same CDS sequence)	NM_001297647.1 , NM_001297647.2
Accession Version	NM_001297647.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2163bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Homo sapiens(human)
Product	connector enhancer of kinase suppressor of ras 1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK296814.1 and BC011604.2. This sequence is a reference standard in the RefSeqGene project. On Jul 10, 2014 this sequence version replaced XM_006710281.1. Transcript Variant: This variant (3) uses an alternate in-frame splice site, compared to variant 1. The encoded isoform (2) is longer than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC011604.2, BT006900.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAACCGG TAGAGACCTG GACCCCCGGA AAGGTGGCAA CTTGGCTGAG AGGTCTTGAC 
GACTCCCTGC AGGACTATCC CTTTGAGGAC TGGCAGCTGC CTGGCAAGAA CCTGCTCCAG 
CTCTGCCCCC AAAGCCTCGA GGCTCTGGCT GTGCGGTCTC TGGGACACCA GGAGCTCATC 
CTGGGCGGGG TGGAACAGCT CCAGGCCCTG AGCTCCAGGC TACAGACAGA GAACCTGCAA 
AGCCTGACAG AGGGACTTCT GGGGGCAACC CATGACTTCC AGAGCATAGT CCAAGGCTGC 
CTGGGGGACT GTGCCAAGAC CCCTATTGAT GTCCTCTGTG CAGCTGTGGA GCTGTTGCAT 
GAAGCTGACG CCCTCCTCTT CTGGCTCAGC AGGTACCTCT TCTCCCACTT AAATGATTTC 
TCAGCATGCC AGGAGATCCG AGACTTGTTG GAGGAGCTGA GCCAGGTCTT GCATGAGGAT 
GGTCCAGCGG CTGAGAAGGA GGGCACAGTC CTGAGGATCT GCAGCCACGT GGCTGGGATC 
TGCCACAACA TCCTGGTCTG CTGCCCCAAG GAGCTGCTGG AACAGAAGGC CGTGCTCGAG 
CAGGTGCAGC TGGACAGTCC ATTGGGCCTA GAAATTCACA CCACCAGCAA TTGCCAGCAC 
TTTGTGTCCC AAGTGGACAC CCAGGTTCCC ACTGACTCCC GACTGCAGAT CCAGCCTGGA 
GACGAGGTTG TCCAGATCAA CGAGCAGGTG GTGGTGCGTG AGGAGAGGGA CATGGTGGGA 
TGGCCCCGTA AGAACATGGT GAGGGAACTG CTGCGGGAGC CAGCCGGACT CAGCTTAGTG 
CTGAAGAAGA TCCCGATACC GGAGACCCCC CCACAGACGC CCCCTCAGGT CCTGGACTCC 
CCGCACCAGA GGAGCCCATC ACTGTCTCTG GCCCCACTGT CTCCCAGGGC CCCATCTGAA 
GACGTCTTTG CCTTTGACCT GTCTTCAAAC CCCAGTCCCG GACCCAGCCC TGCCTGGACA 
GACTCTGCCT CCCTTGGCCC TGAGCCCCTG CCCATCCCCC CGGAACCCCC AGCCATACTC 
CCAGCAGGGG TAGCAGGGAC TCCAGGGCTC CCTGAATCCC CTGACAAGAG TCCTGTTGGT 
CGGAAGAAAT CAAAAGGCCT GGCGACCCGG CTGAGCCGCC GGCGGGTGTC ATGCCGTGAG 
CTGGGCCGGC CGGACTGTGA CGGCTGGCTC CTGTTGCGAA AGGCACCGGG CGGCTTCATG 
GGCCCGCGCT GGCGCCGCCG CTGGTTTGTG CTCAAGGGAC ACACGCTCTA CTGGTACCGC 
CAGCCCCAGG ATGAGAAGGC TGAGGGCCTC ATCAATGTCT CCAACTATAG TCTGGAAAGT 
GGACATGATC AGAAGAAGAA ATATGTGTTT CAGCTCACCC ATGATGTGTA CAAACCCTTC 
ATCTTCGCTG CTGATACCCT GACAGATCTG AGCATGTGGG TGCGTCATCT CATTACCTGC 
ATCTCCAAGT ACCAGTCTCC AGGCCGGGCC CCCCCACCCC GAGAGGAAGA CTGCTACAGT 
GAGACCGAAG CAGAGGACCC GGACGATGAG GCTGGGTCCC ACTCAGCCTC GCCCAGCCCT 
GCTCAAGCTG GGAGTCCCCT CCATGGAGAC ACATCACCTG CAGCCACCCC CACACAGCGC 
AGCCCACGGA CCTCCTTTGG CTCTCTGACA GACAGCAGTG AAGAGGCACT GGAAGGAATG 
GTACGGGGGC TGAGGCAGGG TGGCGTGTCC CTCCTAGGCC AGCCACAGCC CCTGACCCAG 
GAACAGTGGC GGAGCTCTTT CATGCGGCGC AACCGAGACC CTCAGCTCAA TGAGCGAGTG 
CACCGTGTGC GGGCGCTACA GAGCACACTC AAGGCAAAGC TGCAGGAGCT GCAGGTCCTA 
GAAGAAGTGC TGGGTGACCC TGAGCTGACA GGAGAGAAGT TCCGCCAGTG GAAGGAGCAG 
AACCGGGAGC TGTACTCAGA GGGCCTGGGG GCCTGGGGAG TGGCACAGGC TGAAGGCAGC 
TCCCACATCT TGACCTCTGA CTCCACAGAA CAGTCCCCCC ACTCCCTGCC CTCTGACCCT 
GAAGAGCACT CCCATCTCTG CCCCCTGACC TCAGAGAGCA GCCTCCGACC TCCTGACCTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001284576.1
CDS	59..2221
Translation	MEPVETWTPGKVATWLRGLDDSLQDYPFEDWQLPGKNLLQLCPQSLEALAVRSLGHQELILGGVEQLQALSSRLQTENLQSLTEGLLGATHDFQSIVQGCLGDCAKTPIDVLCAAVELLHEADALLFWLSRYLFSHLNDFSACQEIRDLLEELSQVLHEDGPAAEKEGTVLRICSHVAGICHNILVCCPKELLEQKAVLEQVQLDSPLGLEIHTTSNCQHFVSQVDTQVPTDSRLQIQPGDEVVQINEQVVVREERDMVGWPRKNMVRELLREPAGLSLVLKKIPIPETPPQTPPQVLDSPHQRSPSLSLAPLSPRAPSEDVFAFDLSSNPSPGPSPAWTDSASLGPEPLPIPPEPPAILPAGVAGTPGLPESPDKSPVGRKKSKGLATRLSRRRVSCRELGRPDCDGWLLLRKAPGGFMGPRWRRRWFVLKGHTLYWYRQPQDEKAEGLINVSNYSLESGHDQKKKYVFQLTHDVYKPFIFAADTLTDLSMWVRHLITCISKYQSPGRAPPPREEDCYSETEAEDPDDEAGSHSASPSPAQAGSPLHGDTSPAATPTQRSPRTSFGSLTDSSEEALEGMVRGLRQGGVSLLGQPQPLTQEQWRSSFMRRNRDPQLNERVHRVRALQSTLKAKLQELQVLEEVLGDPELTGEKFRQWKEQNRELYSEGLGAWGVAQAEGSSHILTSDSTEQSPHSLPSDPEEHSHLCPLTSESSLRPPDL

Target ORF information:

RefSeq Version	NM_001297647.1
Organism	Homo sapiens(human)
Definition	Homo sapiens connector enhancer of kinase suppressor of Ras 1 (CNKSR1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001297647.1

ORF Insert Sequence:

ATGGAACCGG TAGAGACCTG GACCCCCGGA AAGGTGGCAA CTTGGCTGAG AGGTCTTGAC 
GACTCCCTGC AGGACTATCC CTTTGAGGAC TGGCAGCTGC CTGGCAAGAA CCTGCTCCAG 
CTCTGCCCCC AAAGCCTCGA GGCTCTGGCT GTGCGGTCTC TGGGACACCA GGAGCTCATC 
CTGGGCGGGG TGGAACAGCT CCAGGCCCTG AGCTCCAGGC TACAGACAGA GAACCTGCAA 
AGCCTGACAG AGGGACTTCT GGGGGCAACC CATGACTTCC AGAGCATAGT CCAAGGCTGC 
CTGGGGGACT GTGCCAAGAC CCCTATTGAT GTCCTCTGTG CAGCTGTGGA GCTGTTGCAT 
GAAGCTGACG CCCTCCTCTT CTGGCTCAGC AGGTACCTCT TCTCCCACTT AAATGATTTC 
TCAGCATGCC AGGAGATCCG AGACTTGTTG GAGGAGCTGA GCCAGGTCTT GCATGAGGAT 
GGTCCAGCGG CTGAGAAGGA GGGCACAGTC CTGAGGATCT GCAGCCACGT GGCTGGGATC 
TGCCACAACA TCCTGGTCTG CTGCCCCAAG GAGCTGCTGG AACAGAAGGC CGTGCTCGAG 
CAGGTGCAGC TGGACAGTCC ATTGGGCCTA GAAATTCACA CCACCAGCAA TTGCCAGCAC 
TTTGTGTCCC AAGTGGACAC CCAGGTTCCC ACTGACTCCC GACTGCAGAT CCAGCCTGGA 
GACGAGGTTG TCCAGATCAA CGAGCAGGTG GTGGTGCGTG AGGAGAGGGA CATGGTGGGA 
TGGCCCCGTA AGAACATGGT GAGGGAACTG CTGCGGGAGC CAGCCGGACT CAGCTTAGTG 
CTGAAGAAGA TCCCGATACC GGAGACCCCC CCACAGACGC CCCCTCAGGT CCTGGACTCC 
CCGCACCAGA GGAGCCCATC ACTGTCTCTG GCCCCACTGT CTCCCAGGGC CCCATCTGAA 
GACGTCTTTG CCTTTGACCT GTCTTCAAAC CCCAGTCCCG GACCCAGCCC TGCCTGGACA 
GACTCTGCCT CCCTTGGCCC TGAGCCCCTG CCCATCCCCC CGGAACCCCC AGCCATACTC 
CCAGCAGGGG TAGCAGGGAC TCCAGGGCTC CCTGAATCCC CTGACAAGAG TCCTGTTGGT 
CGGAAGAAAT CAAAAGGCCT GGCGACCCGG CTGAGCCGCC GGCGGGTGTC ATGCCGTGAG 
CTGGGCCGGC CGGACTGTGA CGGCTGGCTC CTGTTGCGAA AGGCACCGGG CGGCTTCATG 
GGCCCGCGCT GGCGCCGCCG CTGGTTTGTG CTCAAGGGAC ACACGCTCTA CTGGTACCGC 
CAGCCCCAGG ATGAGAAGGC TGAGGGCCTC ATCAATGTCT CCAACTATAG TCTGGAAAGT 
GGACATGATC AGAAGAAGAA ATATGTGTTT CAGCTCACCC ATGATGTGTA CAAACCCTTC 
ATCTTCGCTG CTGATACCCT GACAGATCTG AGCATGTGGG TGCGTCATCT CATTACCTGC 
ATCTCCAAGT ACCAGTCTCC AGGCCGGGCC CCCCCACCCC GAGAGGAAGA CTGCTACAGT 
GAGACCGAAG CAGAGGACCC GGACGATGAG GCTGGGTCCC ACTCAGCCTC GCCCAGCCCT 
GCTCAAGCTG GGAGTCCCCT CCATGGAGAC ACATCACCTG CAGCCACCCC CACACAGCGC 
AGCCCACGGA CCTCCTTTGG CTCTCTGACA GACAGCAGTG AAGAGGCACT GGAAGGAATG 
GTACGGGGGC TGAGGCAGGG TGGCGTGTCC CTCCTAGGCC AGCCACAGCC CCTGACCCAG 
GAACAGTGGC GGAGCTCTTT CATGCGGCGC AACCGAGACC CTCAGCTCAA TGAGCGAGTG 
CACCGTGTGC GGGCGCTACA GAGCACACTC AAGGCAAAGC TGCAGGAGCT GCAGGTCCTA 
GAAGAAGTGC TGGGTGACCC TGAGCTGACA GGAGAGAAGT TCCGCCAGTG GAAGGAGCAG 
AACCGGGAGC TGTACTCAGA GGGCCTGGGG GCCTGGGGAG TGGCACAGGC TGAAGGCAGC 
TCCCACATCT TGACCTCTGA CTCCACAGAA CAGTCCCCCC ACTCCCTGCC CTCTGACCCT 
GAAGAGCACT CCCATCTCTG CCCCCTGACC TCAGAGAGCA GCCTCCGACC TCCTGACCTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CNKSR1 ( NM_001297647.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001284576.1 Homo sapiens connector enhancer of kinase suppressor of Ras 1 (CNKSR1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu32910
Clone ID Related Accession (Same CDS sequence)	NM_001297647.1 , NM_001297647.2
Accession Version	NM_001297647.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2163bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	connector enhancer of kinase suppressor of ras 1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK296814.1 and BC011604.2. This sequence is a reference standard in the RefSeqGene project. On May 31, 2019 this sequence version replaced NM_001297647.1. Transcript Variant: This variant (3) uses an alternate in-frame splice site, compared to variant 1. The encoded isoform (2) is longer than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC011604.2, BT006900.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAACCGG TAGAGACCTG GACCCCCGGA AAGGTGGCAA CTTGGCTGAG AGGTCTTGAC 
GACTCCCTGC AGGACTATCC CTTTGAGGAC TGGCAGCTGC CTGGCAAGAA CCTGCTCCAG 
CTCTGCCCCC AAAGCCTCGA GGCTCTGGCT GTGCGGTCTC TGGGACACCA GGAGCTCATC 
CTGGGCGGGG TGGAACAGCT CCAGGCCCTG AGCTCCAGGC TACAGACAGA GAACCTGCAA 
AGCCTGACAG AGGGACTTCT GGGGGCAACC CATGACTTCC AGAGCATAGT CCAAGGCTGC 
CTGGGGGACT GTGCCAAGAC CCCTATTGAT GTCCTCTGTG CAGCTGTGGA GCTGTTGCAT 
GAAGCTGACG CCCTCCTCTT CTGGCTCAGC AGGTACCTCT TCTCCCACTT AAATGATTTC 
TCAGCATGCC AGGAGATCCG AGACTTGTTG GAGGAGCTGA GCCAGGTCTT GCATGAGGAT 
GGTCCAGCGG CTGAGAAGGA GGGCACAGTC CTGAGGATCT GCAGCCACGT GGCTGGGATC 
TGCCACAACA TCCTGGTCTG CTGCCCCAAG GAGCTGCTGG AACAGAAGGC CGTGCTCGAG 
CAGGTGCAGC TGGACAGTCC ATTGGGCCTA GAAATTCACA CCACCAGCAA TTGCCAGCAC 
TTTGTGTCCC AAGTGGACAC CCAGGTTCCC ACTGACTCCC GACTGCAGAT CCAGCCTGGA 
GACGAGGTTG TCCAGATCAA CGAGCAGGTG GTGGTGCGTG AGGAGAGGGA CATGGTGGGA 
TGGCCCCGTA AGAACATGGT GAGGGAACTG CTGCGGGAGC CAGCCGGACT CAGCTTAGTG 
CTGAAGAAGA TCCCGATACC GGAGACCCCC CCACAGACGC CCCCTCAGGT CCTGGACTCC 
CCGCACCAGA GGAGCCCATC ACTGTCTCTG GCCCCACTGT CTCCCAGGGC CCCATCTGAA 
GACGTCTTTG CCTTTGACCT GTCTTCAAAC CCCAGTCCCG GACCCAGCCC TGCCTGGACA 
GACTCTGCCT CCCTTGGCCC TGAGCCCCTG CCCATCCCCC CGGAACCCCC AGCCATACTC 
CCAGCAGGGG TAGCAGGGAC TCCAGGGCTC CCTGAATCCC CTGACAAGAG TCCTGTTGGT 
CGGAAGAAAT CAAAAGGCCT GGCGACCCGG CTGAGCCGCC GGCGGGTGTC ATGCCGTGAG 
CTGGGCCGGC CGGACTGTGA CGGCTGGCTC CTGTTGCGAA AGGCACCGGG CGGCTTCATG 
GGCCCGCGCT GGCGCCGCCG CTGGTTTGTG CTCAAGGGAC ACACGCTCTA CTGGTACCGC 
CAGCCCCAGG ATGAGAAGGC TGAGGGCCTC ATCAATGTCT CCAACTATAG TCTGGAAAGT 
GGACATGATC AGAAGAAGAA ATATGTGTTT CAGCTCACCC ATGATGTGTA CAAACCCTTC 
ATCTTCGCTG CTGATACCCT GACAGATCTG AGCATGTGGG TGCGTCATCT CATTACCTGC 
ATCTCCAAGT ACCAGTCTCC AGGCCGGGCC CCCCCACCCC GAGAGGAAGA CTGCTACAGT 
GAGACCGAAG CAGAGGACCC GGACGATGAG GCTGGGTCCC ACTCAGCCTC GCCCAGCCCT 
GCTCAAGCTG GGAGTCCCCT CCATGGAGAC ACATCACCTG CAGCCACCCC CACACAGCGC 
AGCCCACGGA CCTCCTTTGG CTCTCTGACA GACAGCAGTG AAGAGGCACT GGAAGGAATG 
GTACGGGGGC TGAGGCAGGG TGGCGTGTCC CTCCTAGGCC AGCCACAGCC CCTGACCCAG 
GAACAGTGGC GGAGCTCTTT CATGCGGCGC AACCGAGACC CTCAGCTCAA TGAGCGAGTG 
CACCGTGTGC GGGCGCTACA GAGCACACTC AAGGCAAAGC TGCAGGAGCT GCAGGTCCTA 
GAAGAAGTGC TGGGTGACCC TGAGCTGACA GGAGAGAAGT TCCGCCAGTG GAAGGAGCAG 
AACCGGGAGC TGTACTCAGA GGGCCTGGGG GCCTGGGGAG TGGCACAGGC TGAAGGCAGC 
TCCCACATCT TGACCTCTGA CTCCACAGAA CAGTCCCCCC ACTCCCTGCC CTCTGACCCT 
GAAGAGCACT CCCATCTCTG CCCCCTGACC TCAGAGAGCA GCCTCCGACC TCCTGACCTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001284576.1
CDS	58..2220
Translation	MEPVETWTPGKVATWLRGLDDSLQDYPFEDWQLPGKNLLQLCPQSLEALAVRSLGHQELILGGVEQLQALSSRLQTENLQSLTEGLLGATHDFQSIVQGCLGDCAKTPIDVLCAAVELLHEADALLFWLSRYLFSHLNDFSACQEIRDLLEELSQVLHEDGPAAEKEGTVLRICSHVAGICHNILVCCPKELLEQKAVLEQVQLDSPLGLEIHTTSNCQHFVSQVDTQVPTDSRLQIQPGDEVVQINEQVVVREERDMVGWPRKNMVRELLREPAGLSLVLKKIPIPETPPQTPPQVLDSPHQRSPSLSLAPLSPRAPSEDVFAFDLSSNPSPGPSPAWTDSASLGPEPLPIPPEPPAILPAGVAGTPGLPESPDKSPVGRKKSKGLATRLSRRRVSCRELGRPDCDGWLLLRKAPGGFMGPRWRRRWFVLKGHTLYWYRQPQDEKAEGLINVSNYSLESGHDQKKKYVFQLTHDVYKPFIFAADTLTDLSMWVRHLITCISKYQSPGRAPPPREEDCYSETEAEDPDDEAGSHSASPSPAQAGSPLHGDTSPAATPTQRSPRTSFGSLTDSSEEALEGMVRGLRQGGVSLLGQPQPLTQEQWRSSFMRRNRDPQLNERVHRVRALQSTLKAKLQELQVLEEVLGDPELTGEKFRQWKEQNRELYSEGLGAWGVAQAEGSSHILTSDSTEQSPHSLPSDPEEHSHLCPLTSESSLRPPDL

Target ORF information:

RefSeq Version	NM_001297647.2
Organism	Homo sapiens(human)
Definition	Homo sapiens connector enhancer of kinase suppressor of Ras 1 (CNKSR1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001297647.2

ORF Insert Sequence:

ATGGAACCGG TAGAGACCTG GACCCCCGGA AAGGTGGCAA CTTGGCTGAG AGGTCTTGAC 
GACTCCCTGC AGGACTATCC CTTTGAGGAC TGGCAGCTGC CTGGCAAGAA CCTGCTCCAG 
CTCTGCCCCC AAAGCCTCGA GGCTCTGGCT GTGCGGTCTC TGGGACACCA GGAGCTCATC 
CTGGGCGGGG TGGAACAGCT CCAGGCCCTG AGCTCCAGGC TACAGACAGA GAACCTGCAA 
AGCCTGACAG AGGGACTTCT GGGGGCAACC CATGACTTCC AGAGCATAGT CCAAGGCTGC 
CTGGGGGACT GTGCCAAGAC CCCTATTGAT GTCCTCTGTG CAGCTGTGGA GCTGTTGCAT 
GAAGCTGACG CCCTCCTCTT CTGGCTCAGC AGGTACCTCT TCTCCCACTT AAATGATTTC 
TCAGCATGCC AGGAGATCCG AGACTTGTTG GAGGAGCTGA GCCAGGTCTT GCATGAGGAT 
GGTCCAGCGG CTGAGAAGGA GGGCACAGTC CTGAGGATCT GCAGCCACGT GGCTGGGATC 
TGCCACAACA TCCTGGTCTG CTGCCCCAAG GAGCTGCTGG AACAGAAGGC CGTGCTCGAG 
CAGGTGCAGC TGGACAGTCC ATTGGGCCTA GAAATTCACA CCACCAGCAA TTGCCAGCAC 
TTTGTGTCCC AAGTGGACAC CCAGGTTCCC ACTGACTCCC GACTGCAGAT CCAGCCTGGA 
GACGAGGTTG TCCAGATCAA CGAGCAGGTG GTGGTGCGTG AGGAGAGGGA CATGGTGGGA 
TGGCCCCGTA AGAACATGGT GAGGGAACTG CTGCGGGAGC CAGCCGGACT CAGCTTAGTG 
CTGAAGAAGA TCCCGATACC GGAGACCCCC CCACAGACGC CCCCTCAGGT CCTGGACTCC 
CCGCACCAGA GGAGCCCATC ACTGTCTCTG GCCCCACTGT CTCCCAGGGC CCCATCTGAA 
GACGTCTTTG CCTTTGACCT GTCTTCAAAC CCCAGTCCCG GACCCAGCCC TGCCTGGACA 
GACTCTGCCT CCCTTGGCCC TGAGCCCCTG CCCATCCCCC CGGAACCCCC AGCCATACTC 
CCAGCAGGGG TAGCAGGGAC TCCAGGGCTC CCTGAATCCC CTGACAAGAG TCCTGTTGGT 
CGGAAGAAAT CAAAAGGCCT GGCGACCCGG CTGAGCCGCC GGCGGGTGTC ATGCCGTGAG 
CTGGGCCGGC CGGACTGTGA CGGCTGGCTC CTGTTGCGAA AGGCACCGGG CGGCTTCATG 
GGCCCGCGCT GGCGCCGCCG CTGGTTTGTG CTCAAGGGAC ACACGCTCTA CTGGTACCGC 
CAGCCCCAGG ATGAGAAGGC TGAGGGCCTC ATCAATGTCT CCAACTATAG TCTGGAAAGT 
GGACATGATC AGAAGAAGAA ATATGTGTTT CAGCTCACCC ATGATGTGTA CAAACCCTTC 
ATCTTCGCTG CTGATACCCT GACAGATCTG AGCATGTGGG TGCGTCATCT CATTACCTGC 
ATCTCCAAGT ACCAGTCTCC AGGCCGGGCC CCCCCACCCC GAGAGGAAGA CTGCTACAGT 
GAGACCGAAG CAGAGGACCC GGACGATGAG GCTGGGTCCC ACTCAGCCTC GCCCAGCCCT 
GCTCAAGCTG GGAGTCCCCT CCATGGAGAC ACATCACCTG CAGCCACCCC CACACAGCGC 
AGCCCACGGA CCTCCTTTGG CTCTCTGACA GACAGCAGTG AAGAGGCACT GGAAGGAATG 
GTACGGGGGC TGAGGCAGGG TGGCGTGTCC CTCCTAGGCC AGCCACAGCC CCTGACCCAG 
GAACAGTGGC GGAGCTCTTT CATGCGGCGC AACCGAGACC CTCAGCTCAA TGAGCGAGTG 
CACCGTGTGC GGGCGCTACA GAGCACACTC AAGGCAAAGC TGCAGGAGCT GCAGGTCCTA 
GAAGAAGTGC TGGGTGACCC TGAGCTGACA GGAGAGAAGT TCCGCCAGTG GAAGGAGCAG 
AACCGGGAGC TGTACTCAGA GGGCCTGGGG GCCTGGGGAG TGGCACAGGC TGAAGGCAGC 
TCCCACATCT TGACCTCTGA CTCCACAGAA CAGTCCCCCC ACTCCCTGCC CTCTGACCCT 
GAAGAGCACT CCCATCTCTG CCCCCTGACC TCAGAGAGCA GCCTCCGACC TCCTGACCTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CNKSR1 ( NM_001297648.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001284577.1 Homo sapiens connector enhancer of kinase suppressor of Ras 1 (CNKSR1), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu32911
Clone ID Related Accession (Same CDS sequence)	XM_011540484.2 , NM_001297648.1 , NM_001297648.2
Accession Version	NM_001297648.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1368bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-09-23
Organism	Homo sapiens(human)
Product	connector enhancer of kinase suppressor of ras 1 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK296814.1, AK001900.1, AF100153.1 and BC011604.2. Transcript Variant: This variant (4) uses an alternate splice site in the 5' region and initiates translation at a downstream in-frame start codon, compared to variant 1. The encoded isoform (3) has a shorter N-terminus than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK001900.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142586, SAMEA2144120 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTGAGGG AACTGCTGCG GGAGCCAGCC GGACTCAGCT TAGTGCTGAA GAAGATCCCG 
ATACCGGAGA CCCCCCCACA GACGCCCCCT CAGGTCCTGG ACTCCCCGCA CCAGAGGAGC 
CCATCACTGT CTCTGGCCCC ACTGTCTCCC AGGGCCCCAT CTGAAGACGT CTTTGCCTTT 
GACCTGTCTT CAAACCCCAG TCCCGGACCC AGCCCTGCCT GGACAGACTC TGCCTCCCTT 
GGCCCTGAGC CCCTGCCCAT CCCCCCGGAA CCCCCAGCCA TACTCCCAGC AGGGGTAGCA 
GGGACTCCAG GGCTCCCTGA ATCCCCTGAC AAGAGTCCTG TTGGTCGGAA GAAATCAAAA 
GGCCTGGCGA CCCGGCTGAG CCGCCGGCGG GTGTCATGCC GTGAGCTGGG CCGGCCGGAC 
TGTGACGGCT GGCTCCTGTT GCGAAAGGCA CCGGGCGGCT TCATGGGCCC GCGCTGGCGC 
CGCCGCTGGT TTGTGCTCAA GGGACACACG CTCTACTGGT ACCGCCAGCC CCAGGATGAG 
AAGGCTGAGG GCCTCATCAA TGTCTCCAAC TATAGTCTGG AAAGTGGACA TGATCAGAAG 
AAGAAATATG TGTTTCAGCT CACCCATGAT GTGTACAAAC CCTTCATCTT CGCTGCTGAT 
ACCCTGACAG ATCTGAGCAT GTGGGTGCGT CATCTCATTA CCTGCATCTC CAAGTACCAG 
TCTCCAGGCC GGGCCCCCCC ACCCCGAGAG GAAGACTGCT ACAGTGAGAC CGAAGCAGAG 
GACCCGGACG ATGAGGCTGG GTCCCACTCA GCCTCGCCCA GCCCTGCTCA AGCTGGGAGT 
CCCCTCCATG GAGACACATC ACCTGCAGCC ACCCCCACAC AGCGCAGCCC ACGGACCTCC 
TTTGGCTCTC TGACAGACAG CAGTGAAGAG GCACTGGAAG GAATGGTACG GGGGCTGAGG 
CAGGGTGGCG TGTCCCTCCT AGGCCAGCCA CAGCCCCTGA CCCAGGAACA GTGGCGGAGC 
TCTTTCATGC GGCGCAACCG AGACCCTCAG CTCAATGAGC GAGTGCACCG TGTGCGGGCG 
CTACAGAGCA CACTCAAGGC AAAGCTGCAG GAGCTGCAGG TCCTAGAAGA AGTGCTGGGT 
GACCCTGAGC TGACAGGAGA GAAGTTCCGC CAGTGGAAGG AGCAGAACCG GGAGCTGTAC 
TCAGAGGGCC TGGGGGCCTG GGGAGTGGCA CAGGCTGAAG GCAGCTCCCA CATCTTGACC 
TCTGACTCCA CAGAACAGTC CCCCCACTCC CTGCCCTCTG ACCCTGAAGA GCACTCCCAT 
CTCTGCCCCC TGACCTCAGA GAGCAGCCTC CGACCTCCTG ACCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001284577.1
CDS	828..2195
Translation	MVRELLREPAGLSLVLKKIPIPETPPQTPPQVLDSPHQRSPSLSLAPLSPRAPSEDVFAFDLSSNPSPGPSPAWTDSASLGPEPLPIPPEPPAILPAGVAGTPGLPESPDKSPVGRKKSKGLATRLSRRRVSCRELGRPDCDGWLLLRKAPGGFMGPRWRRRWFVLKGHTLYWYRQPQDEKAEGLINVSNYSLESGHDQKKKYVFQLTHDVYKPFIFAADTLTDLSMWVRHLITCISKYQSPGRAPPPREEDCYSETEAEDPDDEAGSHSASPSPAQAGSPLHGDTSPAATPTQRSPRTSFGSLTDSSEEALEGMVRGLRQGGVSLLGQPQPLTQEQWRSSFMRRNRDPQLNERVHRVRALQSTLKAKLQELQVLEEVLGDPELTGEKFRQWKEQNRELYSEGLGAWGVAQAEGSSHILTSDSTEQSPHSLPSDPEEHSHLCPLTSESSLRPPDL

Target ORF information:

RefSeq Version	NM_001297648.1
Organism	Homo sapiens(human)
Definition	Homo sapiens connector enhancer of kinase suppressor of Ras 1 (CNKSR1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001297648.1

ORF Insert Sequence:

ATGGTGAGGG AACTGCTGCG GGAGCCAGCC GGACTCAGCT TAGTGCTGAA GAAGATCCCG 
ATACCGGAGA CCCCCCCACA GACGCCCCCT CAGGTCCTGG ACTCCCCGCA CCAGAGGAGC 
CCATCACTGT CTCTGGCCCC ACTGTCTCCC AGGGCCCCAT CTGAAGACGT CTTTGCCTTT 
GACCTGTCTT CAAACCCCAG TCCCGGACCC AGCCCTGCCT GGACAGACTC TGCCTCCCTT 
GGCCCTGAGC CCCTGCCCAT CCCCCCGGAA CCCCCAGCCA TACTCCCAGC AGGGGTAGCA 
GGGACTCCAG GGCTCCCTGA ATCCCCTGAC AAGAGTCCTG TTGGTCGGAA GAAATCAAAA 
GGCCTGGCGA CCCGGCTGAG CCGCCGGCGG GTGTCATGCC GTGAGCTGGG CCGGCCGGAC 
TGTGACGGCT GGCTCCTGTT GCGAAAGGCA CCGGGCGGCT TCATGGGCCC GCGCTGGCGC 
CGCCGCTGGT TTGTGCTCAA GGGACACACG CTCTACTGGT ACCGCCAGCC CCAGGATGAG 
AAGGCTGAGG GCCTCATCAA TGTCTCCAAC TATAGTCTGG AAAGTGGACA TGATCAGAAG 
AAGAAATATG TGTTTCAGCT CACCCATGAT GTGTACAAAC CCTTCATCTT CGCTGCTGAT 
ACCCTGACAG ATCTGAGCAT GTGGGTGCGT CATCTCATTA CCTGCATCTC CAAGTACCAG 
TCTCCAGGCC GGGCCCCCCC ACCCCGAGAG GAAGACTGCT ACAGTGAGAC CGAAGCAGAG 
GACCCGGACG ATGAGGCTGG GTCCCACTCA GCCTCGCCCA GCCCTGCTCA AGCTGGGAGT 
CCCCTCCATG GAGACACATC ACCTGCAGCC ACCCCCACAC AGCGCAGCCC ACGGACCTCC 
TTTGGCTCTC TGACAGACAG CAGTGAAGAG GCACTGGAAG GAATGGTACG GGGGCTGAGG 
CAGGGTGGCG TGTCCCTCCT AGGCCAGCCA CAGCCCCTGA CCCAGGAACA GTGGCGGAGC 
TCTTTCATGC GGCGCAACCG AGACCCTCAG CTCAATGAGC GAGTGCACCG TGTGCGGGCG 
CTACAGAGCA CACTCAAGGC AAAGCTGCAG GAGCTGCAGG TCCTAGAAGA AGTGCTGGGT 
GACCCTGAGC TGACAGGAGA GAAGTTCCGC CAGTGGAAGG AGCAGAACCG GGAGCTGTAC 
TCAGAGGGCC TGGGGGCCTG GGGAGTGGCA CAGGCTGAAG GCAGCTCCCA CATCTTGACC 
TCTGACTCCA CAGAACAGTC CCCCCACTCC CTGCCCTCTG ACCCTGAAGA GCACTCCCAT 
CTCTGCCCCC TGACCTCAGA GAGCAGCCTC CGACCTCCTG ACCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CNKSR1 ( NM_001297648.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001284577.1 Homo sapiens connector enhancer of kinase suppressor of Ras 1 (CNKSR1), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu32911
Clone ID Related Accession (Same CDS sequence)	XM_011540484.2 , NM_001297648.1 , NM_001297648.2
Accession Version	NM_001297648.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1368bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	connector enhancer of kinase suppressor of ras 1 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK296814.1, AK001900.1, AF100153.1 and BC011604.2. On May 31, 2019 this sequence version replaced NM_001297648.1. Transcript Variant: This variant (4) uses an alternate splice site in the 5' region and initiates translation at a downstream in-frame start codon, compared to variant 1. The encoded isoform (3) has a shorter N-terminus than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR7346977.954169.1, AK001900.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142586, SAMEA2144120 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTGAGGG AACTGCTGCG GGAGCCAGCC GGACTCAGCT TAGTGCTGAA GAAGATCCCG 
ATACCGGAGA CCCCCCCACA GACGCCCCCT CAGGTCCTGG ACTCCCCGCA CCAGAGGAGC 
CCATCACTGT CTCTGGCCCC ACTGTCTCCC AGGGCCCCAT CTGAAGACGT CTTTGCCTTT 
GACCTGTCTT CAAACCCCAG TCCCGGACCC AGCCCTGCCT GGACAGACTC TGCCTCCCTT 
GGCCCTGAGC CCCTGCCCAT CCCCCCGGAA CCCCCAGCCA TACTCCCAGC AGGGGTAGCA 
GGGACTCCAG GGCTCCCTGA ATCCCCTGAC AAGAGTCCTG TTGGTCGGAA GAAATCAAAA 
GGCCTGGCGA CCCGGCTGAG CCGCCGGCGG GTGTCATGCC GTGAGCTGGG CCGGCCGGAC 
TGTGACGGCT GGCTCCTGTT GCGAAAGGCA CCGGGCGGCT TCATGGGCCC GCGCTGGCGC 
CGCCGCTGGT TTGTGCTCAA GGGACACACG CTCTACTGGT ACCGCCAGCC CCAGGATGAG 
AAGGCTGAGG GCCTCATCAA TGTCTCCAAC TATAGTCTGG AAAGTGGACA TGATCAGAAG 
AAGAAATATG TGTTTCAGCT CACCCATGAT GTGTACAAAC CCTTCATCTT CGCTGCTGAT 
ACCCTGACAG ATCTGAGCAT GTGGGTGCGT CATCTCATTA CCTGCATCTC CAAGTACCAG 
TCTCCAGGCC GGGCCCCCCC ACCCCGAGAG GAAGACTGCT ACAGTGAGAC CGAAGCAGAG 
GACCCGGACG ATGAGGCTGG GTCCCACTCA GCCTCGCCCA GCCCTGCTCA AGCTGGGAGT 
CCCCTCCATG GAGACACATC ACCTGCAGCC ACCCCCACAC AGCGCAGCCC ACGGACCTCC 
TTTGGCTCTC TGACAGACAG CAGTGAAGAG GCACTGGAAG GAATGGTACG GGGGCTGAGG 
CAGGGTGGCG TGTCCCTCCT AGGCCAGCCA CAGCCCCTGA CCCAGGAACA GTGGCGGAGC 
TCTTTCATGC GGCGCAACCG AGACCCTCAG CTCAATGAGC GAGTGCACCG TGTGCGGGCG 
CTACAGAGCA CACTCAAGGC AAAGCTGCAG GAGCTGCAGG TCCTAGAAGA AGTGCTGGGT 
GACCCTGAGC TGACAGGAGA GAAGTTCCGC CAGTGGAAGG AGCAGAACCG GGAGCTGTAC 
TCAGAGGGCC TGGGGGCCTG GGGAGTGGCA CAGGCTGAAG GCAGCTCCCA CATCTTGACC 
TCTGACTCCA CAGAACAGTC CCCCCACTCC CTGCCCTCTG ACCCTGAAGA GCACTCCCAT 
CTCTGCCCCC TGACCTCAGA GAGCAGCCTC CGACCTCCTG ACCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001284577.1
CDS	827..2194
Translation	MVRELLREPAGLSLVLKKIPIPETPPQTPPQVLDSPHQRSPSLSLAPLSPRAPSEDVFAFDLSSNPSPGPSPAWTDSASLGPEPLPIPPEPPAILPAGVAGTPGLPESPDKSPVGRKKSKGLATRLSRRRVSCRELGRPDCDGWLLLRKAPGGFMGPRWRRRWFVLKGHTLYWYRQPQDEKAEGLINVSNYSLESGHDQKKKYVFQLTHDVYKPFIFAADTLTDLSMWVRHLITCISKYQSPGRAPPPREEDCYSETEAEDPDDEAGSHSASPSPAQAGSPLHGDTSPAATPTQRSPRTSFGSLTDSSEEALEGMVRGLRQGGVSLLGQPQPLTQEQWRSSFMRRNRDPQLNERVHRVRALQSTLKAKLQELQVLEEVLGDPELTGEKFRQWKEQNRELYSEGLGAWGVAQAEGSSHILTSDSTEQSPHSLPSDPEEHSHLCPLTSESSLRPPDL

Target ORF information:

RefSeq Version	NM_001297648.2
Organism	Homo sapiens(human)
Definition	Homo sapiens connector enhancer of kinase suppressor of Ras 1 (CNKSR1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001297648.2

ORF Insert Sequence:

ATGGTGAGGG AACTGCTGCG GGAGCCAGCC GGACTCAGCT TAGTGCTGAA GAAGATCCCG 
ATACCGGAGA CCCCCCCACA GACGCCCCCT CAGGTCCTGG ACTCCCCGCA CCAGAGGAGC 
CCATCACTGT CTCTGGCCCC ACTGTCTCCC AGGGCCCCAT CTGAAGACGT CTTTGCCTTT 
GACCTGTCTT CAAACCCCAG TCCCGGACCC AGCCCTGCCT GGACAGACTC TGCCTCCCTT 
GGCCCTGAGC CCCTGCCCAT CCCCCCGGAA CCCCCAGCCA TACTCCCAGC AGGGGTAGCA 
GGGACTCCAG GGCTCCCTGA ATCCCCTGAC AAGAGTCCTG TTGGTCGGAA GAAATCAAAA 
GGCCTGGCGA CCCGGCTGAG CCGCCGGCGG GTGTCATGCC GTGAGCTGGG CCGGCCGGAC 
TGTGACGGCT GGCTCCTGTT GCGAAAGGCA CCGGGCGGCT TCATGGGCCC GCGCTGGCGC 
CGCCGCTGGT TTGTGCTCAA GGGACACACG CTCTACTGGT ACCGCCAGCC CCAGGATGAG 
AAGGCTGAGG GCCTCATCAA TGTCTCCAAC TATAGTCTGG AAAGTGGACA TGATCAGAAG 
AAGAAATATG TGTTTCAGCT CACCCATGAT GTGTACAAAC CCTTCATCTT CGCTGCTGAT 
ACCCTGACAG ATCTGAGCAT GTGGGTGCGT CATCTCATTA CCTGCATCTC CAAGTACCAG 
TCTCCAGGCC GGGCCCCCCC ACCCCGAGAG GAAGACTGCT ACAGTGAGAC CGAAGCAGAG 
GACCCGGACG ATGAGGCTGG GTCCCACTCA GCCTCGCCCA GCCCTGCTCA AGCTGGGAGT 
CCCCTCCATG GAGACACATC ACCTGCAGCC ACCCCCACAC AGCGCAGCCC ACGGACCTCC 
TTTGGCTCTC TGACAGACAG CAGTGAAGAG GCACTGGAAG GAATGGTACG GGGGCTGAGG 
CAGGGTGGCG TGTCCCTCCT AGGCCAGCCA CAGCCCCTGA CCCAGGAACA GTGGCGGAGC 
TCTTTCATGC GGCGCAACCG AGACCCTCAG CTCAATGAGC GAGTGCACCG TGTGCGGGCG 
CTACAGAGCA CACTCAAGGC AAAGCTGCAG GAGCTGCAGG TCCTAGAAGA AGTGCTGGGT 
GACCCTGAGC TGACAGGAGA GAAGTTCCGC CAGTGGAAGG AGCAGAACCG GGAGCTGTAC 
TCAGAGGGCC TGGGGGCCTG GGGAGTGGCA CAGGCTGAAG GCAGCTCCCA CATCTTGACC 
TCTGACTCCA CAGAACAGTC CCCCCACTCC CTGCCCTCTG ACCCTGAAGA GCACTCCCAT 
CTCTGCCCCC TGACCTCAGA GAGCAGCCTC CGACCTCCTG ACCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CNKSR1 ( XM_011540484.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011538786.1 Homo sapiens connector enhancer of kinase suppressor of Ras 1 (CNKSR1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu32911
Clone ID Related Accession (Same CDS sequence)	XM_011540484.2 , NM_001297648.1 , NM_001297648.2
Accession Version	XM_011540484.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1368bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	connector enhancer of kinase suppressor of ras 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011540484.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAGGG AACTGCTGCG GGAGCCAGCC GGACTCAGCT TAGTGCTGAA GAAGATCCCG 
ATACCGGAGA CCCCCCCACA GACGCCCCCT CAGGTCCTGG ACTCCCCGCA CCAGAGGAGC 
CCATCACTGT CTCTGGCCCC ACTGTCTCCC AGGGCCCCAT CTGAAGACGT CTTTGCCTTT 
GACCTGTCTT CAAACCCCAG TCCCGGACCC AGCCCTGCCT GGACAGACTC TGCCTCCCTT 
GGCCCTGAGC CCCTGCCCAT CCCCCCGGAA CCCCCAGCCA TACTCCCAGC AGGGGTAGCA 
GGGACTCCAG GGCTCCCTGA ATCCCCTGAC AAGAGTCCTG TTGGTCGGAA GAAATCAAAA 
GGCCTGGCGA CCCGGCTGAG CCGCCGGCGG GTGTCATGCC GTGAGCTGGG CCGGCCGGAC 
TGTGACGGCT GGCTCCTGTT GCGAAAGGCA CCGGGCGGCT TCATGGGCCC GCGCTGGCGC 
CGCCGCTGGT TTGTGCTCAA GGGACACACG CTCTACTGGT ACCGCCAGCC CCAGGATGAG 
AAGGCTGAGG GCCTCATCAA TGTCTCCAAC TATAGTCTGG AAAGTGGACA TGATCAGAAG 
AAGAAATATG TGTTTCAGCT CACCCATGAT GTGTACAAAC CCTTCATCTT CGCTGCTGAT 
ACCCTGACAG ATCTGAGCAT GTGGGTGCGT CATCTCATTA CCTGCATCTC CAAGTACCAG 
TCTCCAGGCC GGGCCCCCCC ACCCCGAGAG GAAGACTGCT ACAGTGAGAC CGAAGCAGAG 
GACCCGGACG ATGAGGCTGG GTCCCACTCA GCCTCGCCCA GCCCTGCTCA AGCTGGGAGT 
CCCCTCCATG GAGACACATC ACCTGCAGCC ACCCCCACAC AGCGCAGCCC ACGGACCTCC 
TTTGGCTCTC TGACAGACAG CAGTGAAGAG GCACTGGAAG GAATGGTACG GGGGCTGAGG 
CAGGGTGGCG TGTCCCTCCT AGGCCAGCCA CAGCCCCTGA CCCAGGAACA GTGGCGGAGC 
TCTTTCATGC GGCGCAACCG AGACCCTCAG CTCAATGAGC GAGTGCACCG TGTGCGGGCG 
CTACAGAGCA CACTCAAGGC AAAGCTGCAG GAGCTGCAGG TCCTAGAAGA AGTGCTGGGT 
GACCCTGAGC TGACAGGAGA GAAGTTCCGC CAGTGGAAGG AGCAGAACCG GGAGCTGTAC 
TCAGAGGGCC TGGGGGCCTG GGGAGTGGCA CAGGCTGAAG GCAGCTCCCA CATCTTGACC 
TCTGACTCCA CAGAACAGTC CCCCCACTCC CTGCCCTCTG ACCCTGAAGA GCACTCCCAT 
CTCTGCCCCC TGACCTCAGA GAGCAGCCTC CGACCTCCTG ACCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011538786.1
CDS	813..2180
Translation	MVRELLREPAGLSLVLKKIPIPETPPQTPPQVLDSPHQRSPSLSLAPLSPRAPSEDVFAFDLSSNPSPGPSPAWTDSASLGPEPLPIPPEPPAILPAGVAGTPGLPESPDKSPVGRKKSKGLATRLSRRRVSCRELGRPDCDGWLLLRKAPGGFMGPRWRRRWFVLKGHTLYWYRQPQDEKAEGLINVSNYSLESGHDQKKKYVFQLTHDVYKPFIFAADTLTDLSMWVRHLITCISKYQSPGRAPPPREEDCYSETEAEDPDDEAGSHSASPSPAQAGSPLHGDTSPAATPTQRSPRTSFGSLTDSSEEALEGMVRGLRQGGVSLLGQPQPLTQEQWRSSFMRRNRDPQLNERVHRVRALQSTLKAKLQELQVLEEVLGDPELTGEKFRQWKEQNRELYSEGLGAWGVAQAEGSSHILTSDSTEQSPHSLPSDPEEHSHLCPLTSESSLRPPDL

Target ORF information:

RefSeq Version	XM_011540484.2
Organism	Homo sapiens(human)
Definition	Homo sapiens connector enhancer of kinase suppressor of Ras 1 (CNKSR1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011540484.2

ORF Insert Sequence:

ATGGTGAGGG AACTGCTGCG GGAGCCAGCC GGACTCAGCT TAGTGCTGAA GAAGATCCCG 
ATACCGGAGA CCCCCCCACA GACGCCCCCT CAGGTCCTGG ACTCCCCGCA CCAGAGGAGC 
CCATCACTGT CTCTGGCCCC ACTGTCTCCC AGGGCCCCAT CTGAAGACGT CTTTGCCTTT 
GACCTGTCTT CAAACCCCAG TCCCGGACCC AGCCCTGCCT GGACAGACTC TGCCTCCCTT 
GGCCCTGAGC CCCTGCCCAT CCCCCCGGAA CCCCCAGCCA TACTCCCAGC AGGGGTAGCA 
GGGACTCCAG GGCTCCCTGA ATCCCCTGAC AAGAGTCCTG TTGGTCGGAA GAAATCAAAA 
GGCCTGGCGA CCCGGCTGAG CCGCCGGCGG GTGTCATGCC GTGAGCTGGG CCGGCCGGAC 
TGTGACGGCT GGCTCCTGTT GCGAAAGGCA CCGGGCGGCT TCATGGGCCC GCGCTGGCGC 
CGCCGCTGGT TTGTGCTCAA GGGACACACG CTCTACTGGT ACCGCCAGCC CCAGGATGAG 
AAGGCTGAGG GCCTCATCAA TGTCTCCAAC TATAGTCTGG AAAGTGGACA TGATCAGAAG 
AAGAAATATG TGTTTCAGCT CACCCATGAT GTGTACAAAC CCTTCATCTT CGCTGCTGAT 
ACCCTGACAG ATCTGAGCAT GTGGGTGCGT CATCTCATTA CCTGCATCTC CAAGTACCAG 
TCTCCAGGCC GGGCCCCCCC ACCCCGAGAG GAAGACTGCT ACAGTGAGAC CGAAGCAGAG 
GACCCGGACG ATGAGGCTGG GTCCCACTCA GCCTCGCCCA GCCCTGCTCA AGCTGGGAGT 
CCCCTCCATG GAGACACATC ACCTGCAGCC ACCCCCACAC AGCGCAGCCC ACGGACCTCC 
TTTGGCTCTC TGACAGACAG CAGTGAAGAG GCACTGGAAG GAATGGTACG GGGGCTGAGG 
CAGGGTGGCG TGTCCCTCCT AGGCCAGCCA CAGCCCCTGA CCCAGGAACA GTGGCGGAGC 
TCTTTCATGC GGCGCAACCG AGACCCTCAG CTCAATGAGC GAGTGCACCG TGTGCGGGCG 
CTACAGAGCA CACTCAAGGC AAAGCTGCAG GAGCTGCAGG TCCTAGAAGA AGTGCTGGGT 
GACCCTGAGC TGACAGGAGA GAAGTTCCGC CAGTGGAAGG AGCAGAACCG GGAGCTGTAC 
TCAGAGGGCC TGGGGGCCTG GGGAGTGGCA CAGGCTGAAG GCAGCTCCCA CATCTTGACC 
TCTGACTCCA CAGAACAGTC CCCCCACTCC CTGCCCTCTG ACCCTGAAGA GCACTCCCAT 
CTCTGCCCCC TGACCTCAGA GAGCAGCCTC CGACCTCCTG ACCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CNKSR1 ( XM_011540485.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011538787.1 Homo sapiens connector enhancer of kinase suppressor of Ras 1 (CNKSR1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu63818
Clone ID Related Accession (Same CDS sequence)	XM_011540485.2
Accession Version	XM_011540485.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1392bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	connector enhancer of kinase suppressor of ras 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011540485.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGGAT GGCCCCGTAA GAACATGGTG AGGGAACTGC TGCGGGAGCC AGCCGGACTC 
AGCTTAGTGC TGAAGAAGAT CCCGATACCG GAGACCCCCC CACAGACGCC CCCTCAGGTC 
CTGGACTCCC CGCACCAGAG GAGCCCATCA CTGTCTCTGG CCCCACTGTC TCCCAGGGCC 
CCATCTGAAG ACGTCTTTGC CTTTGACCTG TCTTCAAACC CCAGTCCCGG ACCCAGCCCT 
GCCTGGACAG ACTCTGCCTC CCTTGGCCCT GAGCCCCTGC CCATCCCCCC GGAACCCCCA 
GCCATACTCC CAGCAGGGGT AGCAGGGACT CCAGGGCTCC CTGAATCCCC TGACAAGAGT 
CCTGTTGGTC GGAAGAAATC AAAAGGCCTG GCGACCCGGC TGAGCCGCCG GCGGGTGTCA 
TGCCGTGAGC TGGGCCGGCC GGACTGTGAC GGCTGGCTCC TGTTGCGAAA GGCACCGGGC 
GGCTTCATGG GCCCGCGCTG GCGCCGCCGC TGGTTTGTGC TCAAGGGACA CACGCTCTAC 
TGGTACCGCC AGCCCCAGGA TGAGAAGGCT GAGGGCCTCA TCAATGTCTC CAACTATAGT 
CTGGAAAGTG GACATGATCA GAAGAAGAAA TATGTGTTTC AGCTCACCCA TGATGTGTAC 
AAACCCTTCA TCTTCGCTGC TGATACCCTG ACAGATCTGA GCATGTGGGT GCGTCATCTC 
ATTACCTGCA TCTCCAAGTA CCAGTCTCCA GGCCGGGCCC CCCCACCCCG AGAGGAAGAC 
TGCTACAGTG AGACCGAAGC AGAGGACCCG GACGATGAGG CTGGGTCCCA CTCAGCCTCG 
CCCAGCCCTG CTCAAGCTGG GAGTCCCCTC CATGGAGACA CATCACCTGC AGCCACCCCC 
ACACAGCGCA GCCCACGGAC CTCCTTTGGC TCTCTGACAG ACAGCAGTGA AGAGGCACTG 
GAAGGAATGG TACGGGGGCT GAGGCAGGGT GGCGTGTCCC TCCTAGGCCA GCCACAGCCC 
CTGACCCAGG AACAGTGGCG GAGCTCTTTC ATGCGGCGCA ACCGAGACCC TCAGCTCAAT 
GAGCGAGTGC ACCGTGTGCG GGCGCTACAG AGCACACTCA AGGCAAAGCT GCAGGAGCTG 
CAGGTCCTAG AAGAAGTGCT GGGTGACCCT GAGCTGACAG GAGAGAAGTT CCGCCAGTGG 
AAGGAGCAGA ACCGGGAGCT GTACTCAGAG GGCCTGGGGG CCTGGGGAGT GGCACAGGCT 
GAAGGCAGCT CCCACATCTT GACCTCTGAC TCCACAGAAC AGTCCCCCCA CTCCCTGCCC 
TCTGACCCTG AAGAGCACTC CCATCTCTGC CCCCTGACCT CAGAGAGCAG CCTCCGACCT 
CCTGACCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011538787.1
CDS	319..1710
Translation	MVGWPRKNMVRELLREPAGLSLVLKKIPIPETPPQTPPQVLDSPHQRSPSLSLAPLSPRAPSEDVFAFDLSSNPSPGPSPAWTDSASLGPEPLPIPPEPPAILPAGVAGTPGLPESPDKSPVGRKKSKGLATRLSRRRVSCRELGRPDCDGWLLLRKAPGGFMGPRWRRRWFVLKGHTLYWYRQPQDEKAEGLINVSNYSLESGHDQKKKYVFQLTHDVYKPFIFAADTLTDLSMWVRHLITCISKYQSPGRAPPPREEDCYSETEAEDPDDEAGSHSASPSPAQAGSPLHGDTSPAATPTQRSPRTSFGSLTDSSEEALEGMVRGLRQGGVSLLGQPQPLTQEQWRSSFMRRNRDPQLNERVHRVRALQSTLKAKLQELQVLEEVLGDPELTGEKFRQWKEQNRELYSEGLGAWGVAQAEGSSHILTSDSTEQSPHSLPSDPEEHSHLCPLTSESSLRPPDL

Target ORF information:

RefSeq Version	XM_011540485.2
Organism	Homo sapiens(human)
Definition	Homo sapiens connector enhancer of kinase suppressor of Ras 1 (CNKSR1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011540485.2

ORF Insert Sequence:

ATGGTGGGAT GGCCCCGTAA GAACATGGTG AGGGAACTGC TGCGGGAGCC AGCCGGACTC 
AGCTTAGTGC TGAAGAAGAT CCCGATACCG GAGACCCCCC CACAGACGCC CCCTCAGGTC 
CTGGACTCCC CGCACCAGAG GAGCCCATCA CTGTCTCTGG CCCCACTGTC TCCCAGGGCC 
CCATCTGAAG ACGTCTTTGC CTTTGACCTG TCTTCAAACC CCAGTCCCGG ACCCAGCCCT 
GCCTGGACAG ACTCTGCCTC CCTTGGCCCT GAGCCCCTGC CCATCCCCCC GGAACCCCCA 
GCCATACTCC CAGCAGGGGT AGCAGGGACT CCAGGGCTCC CTGAATCCCC TGACAAGAGT 
CCTGTTGGTC GGAAGAAATC AAAAGGCCTG GCGACCCGGC TGAGCCGCCG GCGGGTGTCA 
TGCCGTGAGC TGGGCCGGCC GGACTGTGAC GGCTGGCTCC TGTTGCGAAA GGCACCGGGC 
GGCTTCATGG GCCCGCGCTG GCGCCGCCGC TGGTTTGTGC TCAAGGGACA CACGCTCTAC 
TGGTACCGCC AGCCCCAGGA TGAGAAGGCT GAGGGCCTCA TCAATGTCTC CAACTATAGT 
CTGGAAAGTG GACATGATCA GAAGAAGAAA TATGTGTTTC AGCTCACCCA TGATGTGTAC 
AAACCCTTCA TCTTCGCTGC TGATACCCTG ACAGATCTGA GCATGTGGGT GCGTCATCTC 
ATTACCTGCA TCTCCAAGTA CCAGTCTCCA GGCCGGGCCC CCCCACCCCG AGAGGAAGAC 
TGCTACAGTG AGACCGAAGC AGAGGACCCG GACGATGAGG CTGGGTCCCA CTCAGCCTCG 
CCCAGCCCTG CTCAAGCTGG GAGTCCCCTC CATGGAGACA CATCACCTGC AGCCACCCCC 
ACACAGCGCA GCCCACGGAC CTCCTTTGGC TCTCTGACAG ACAGCAGTGA AGAGGCACTG 
GAAGGAATGG TACGGGGGCT GAGGCAGGGT GGCGTGTCCC TCCTAGGCCA GCCACAGCCC 
CTGACCCAGG AACAGTGGCG GAGCTCTTTC ATGCGGCGCA ACCGAGACCC TCAGCTCAAT 
GAGCGAGTGC ACCGTGTGCG GGCGCTACAG AGCACACTCA AGGCAAAGCT GCAGGAGCTG 
CAGGTCCTAG AAGAAGTGCT GGGTGACCCT GAGCTGACAG GAGAGAAGTT CCGCCAGTGG 
AAGGAGCAGA ACCGGGAGCT GTACTCAGAG GGCCTGGGGG CCTGGGGAGT GGCACAGGCT 
GAAGGCAGCT CCCACATCTT GACCTCTGAC TCCACAGAAC AGTCCCCCCA CTCCCTGCCC 
TCTGACCCTG AAGAGCACTC CCATCTCTGC CCCCTGACCT CAGAGAGCAG CCTCCGACCT 
CCTGACCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CNKSR1 ( NM_006314.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_006305.2 Homo sapiens connector enhancer of kinase suppressor of Ras 1 (CNKSR1), transcript variant 1, mRNA.

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CloneID	OHu31462
Clone ID Related Accession (Same CDS sequence)	NM_006314.3 , NM_006314.2
Accession Version	NM_006314.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2142bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	connector enhancer of kinase suppressor of ras 1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA445069.1, AF100153.1 and BC011604.2. On Nov 23, 2018 this sequence version replaced NM_006314.2. Transcript Variant: This variant (1) encodes isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF100153.1, AB209335.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000361530.11/ ENSP00000354609.6 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGAACCGG TAGAGACCTG GACCCCCGGA AAGGTGGCAA CTTGGCTGAG AGGTCTTGAC 
GACTCCCTGC AGGACTATCC CTTTGAGGAC TGGCAGCTGC CTGGCAAGAA CCTGCTCCAG 
CTCTGCCCCC AAAGCCTCGA GGCTCTGGCT GTGCGGTCTC TGGGACACCA GGAGCTCATC 
CTGGGCGGGG TGGAACAGCT CCAGGCCCTG AGCTCCAGGC TACAGACAGA GAACCTGCAA 
AGCCTGACAG AGGGACTTCT GGGGGCAACC CATGACTTCC AGAGCATAGT CCAAGGCTGC 
CTGGGGGACT GTGCCAAGAC CCCTATTGAT GTCCTCTGTG CAGCTGTGGA GCTGTTGCAT 
GAAGCTGACG CCCTCCTCTT CTGGCTCAGC AGGTACCTCT TCTCCCACTT AAATGATTTC 
TCAGCATGCC AGGAGATCCG AGACTTGTTG GAGGAGCTGA GCCAGGTCTT GCATGAGGAT 
GGTCCAGCGG CTGAGAAGGA GGGCACAGTC CTGAGGATCT GCAGCCACGT GGCTGGGATC 
TGCCACAACA TCCTGGTCTG CTGCCCCAAG GAGCTGCTGG AACAGAAGGC CGTGCTCGAG 
CAGGTGCAGC TGGACAGTCC ATTGGGCCTA GAAATTCACA CCACCAGCAA TTGCCAGCAC 
TTTGTGTCCC AAGTGGACAC CCAGGTTCCC ACTGACTCCC GACTGCAGAT CCAGCCTGGA 
GACGAGGTTG TCCAGATCAA CGAGCAGGTG GTGGTGGGAT GGCCCCGTAA GAACATGGTG 
AGGGAACTGC TGCGGGAGCC AGCCGGACTC AGCTTAGTGC TGAAGAAGAT CCCGATACCG 
GAGACCCCCC CACAGACGCC CCCTCAGGTC CTGGACTCCC CGCACCAGAG GAGCCCATCA 
CTGTCTCTGG CCCCACTGTC TCCCAGGGCC CCATCTGAAG ACGTCTTTGC CTTTGACCTG 
TCTTCAAACC CCAGTCCCGG ACCCAGCCCT GCCTGGACAG ACTCTGCCTC CCTTGGCCCT 
GAGCCCCTGC CCATCCCCCC GGAACCCCCA GCCATACTCC CAGCAGGGGT AGCAGGGACT 
CCAGGGCTCC CTGAATCCCC TGACAAGAGT CCTGTTGGTC GGAAGAAATC AAAAGGCCTG 
GCGACCCGGC TGAGCCGCCG GCGGGTGTCA TGCCGTGAGC TGGGCCGGCC GGACTGTGAC 
GGCTGGCTCC TGTTGCGAAA GGCACCGGGC GGCTTCATGG GCCCGCGCTG GCGCCGCCGC 
TGGTTTGTGC TCAAGGGACA CACGCTCTAC TGGTACCGCC AGCCCCAGGA TGAGAAGGCT 
GAGGGCCTCA TCAATGTCTC CAACTATAGT CTGGAAAGTG GACATGATCA GAAGAAGAAA 
TATGTGTTTC AGCTCACCCA TGATGTGTAC AAACCCTTCA TCTTCGCTGC TGATACCCTG 
ACAGATCTGA GCATGTGGGT GCGTCATCTC ATTACCTGCA TCTCCAAGTA CCAGTCTCCA 
GGCCGGGCCC CCCCACCCCG AGAGGAAGAC TGCTACAGTG AGACCGAAGC AGAGGACCCG 
GACGATGAGG CTGGGTCCCA CTCAGCCTCG CCCAGCCCTG CTCAAGCTGG GAGTCCCCTC 
CATGGAGACA CATCACCTGC AGCCACCCCC ACACAGCGCA GCCCACGGAC CTCCTTTGGC 
TCTCTGACAG ACAGCAGTGA AGAGGCACTG GAAGGAATGG TACGGGGGCT GAGGCAGGGT 
GGCGTGTCCC TCCTAGGCCA GCCACAGCCC CTGACCCAGG AACAGTGGCG GAGCTCTTTC 
ATGCGGCGCA ACCGAGACCC TCAGCTCAAT GAGCGAGTGC ACCGTGTGCG GGCGCTACAG 
AGCACACTCA AGGCAAAGCT GCAGGAGCTG CAGGTCCTAG AAGAAGTGCT GGGTGACCCT 
GAGCTGACAG GAGAGAAGTT CCGCCAGTGG AAGGAGCAGA ACCGGGAGCT GTACTCAGAG 
GGCCTGGGGG CCTGGGGAGT GGCACAGGCT GAAGGCAGCT CCCACATCTT GACCTCTGAC 
TCCACAGAAC AGTCCCCCCA CTCCCTGCCC TCTGACCCTG AAGAGCACTC CCATCTCTGC 
CCCCTGACCT CAGAGAGCAG CCTCCGACCT CCTGACCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_006305.2
CDS	58..2199
Translation	MEPVETWTPGKVATWLRGLDDSLQDYPFEDWQLPGKNLLQLCPQSLEALAVRSLGHQELILGGVEQLQALSSRLQTENLQSLTEGLLGATHDFQSIVQGCLGDCAKTPIDVLCAAVELLHEADALLFWLSRYLFSHLNDFSACQEIRDLLEELSQVLHEDGPAAEKEGTVLRICSHVAGICHNILVCCPKELLEQKAVLEQVQLDSPLGLEIHTTSNCQHFVSQVDTQVPTDSRLQIQPGDEVVQINEQVVVGWPRKNMVRELLREPAGLSLVLKKIPIPETPPQTPPQVLDSPHQRSPSLSLAPLSPRAPSEDVFAFDLSSNPSPGPSPAWTDSASLGPEPLPIPPEPPAILPAGVAGTPGLPESPDKSPVGRKKSKGLATRLSRRRVSCRELGRPDCDGWLLLRKAPGGFMGPRWRRRWFVLKGHTLYWYRQPQDEKAEGLINVSNYSLESGHDQKKKYVFQLTHDVYKPFIFAADTLTDLSMWVRHLITCISKYQSPGRAPPPREEDCYSETEAEDPDDEAGSHSASPSPAQAGSPLHGDTSPAATPTQRSPRTSFGSLTDSSEEALEGMVRGLRQGGVSLLGQPQPLTQEQWRSSFMRRNRDPQLNERVHRVRALQSTLKAKLQELQVLEEVLGDPELTGEKFRQWKEQNRELYSEGLGAWGVAQAEGSSHILTSDSTEQSPHSLPSDPEEHSHLCPLTSESSLRPPDL

Target ORF information:

RefSeq Version	NM_006314.3
Organism	Homo sapiens(human)
Definition	Homo sapiens connector enhancer of kinase suppressor of Ras 1 (CNKSR1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006314.3

ORF Insert Sequence:

ATGGAACCGG TAGAGACCTG GACCCCCGGA AAGGTGGCAA CTTGGCTGAG AGGTCTTGAC 
GACTCCCTGC AGGACTATCC CTTTGAGGAC TGGCAGCTGC CTGGCAAGAA CCTGCTCCAG 
CTCTGCCCCC AAAGCCTCGA GGCTCTGGCT GTGCGGTCTC TGGGACACCA GGAGCTCATC 
CTGGGCGGGG TGGAACAGCT CCAGGCCCTG AGCTCCAGGC TACAGACAGA GAACCTGCAA 
AGCCTGACAG AGGGACTTCT GGGGGCAACC CATGACTTCC AGAGCATAGT CCAAGGCTGC 
CTGGGGGACT GTGCCAAGAC CCCTATTGAT GTCCTCTGTG CAGCTGTGGA GCTGTTGCAT 
GAAGCTGACG CCCTCCTCTT CTGGCTCAGC AGGTACCTCT TCTCCCACTT AAATGATTTC 
TCAGCATGCC AGGAGATCCG AGACTTGTTG GAGGAGCTGA GCCAGGTCTT GCATGAGGAT 
GGTCCAGCGG CTGAGAAGGA GGGCACAGTC CTGAGGATCT GCAGCCACGT GGCTGGGATC 
TGCCACAACA TCCTGGTCTG CTGCCCCAAG GAGCTGCTGG AACAGAAGGC CGTGCTCGAG 
CAGGTGCAGC TGGACAGTCC ATTGGGCCTA GAAATTCACA CCACCAGCAA TTGCCAGCAC 
TTTGTGTCCC AAGTGGACAC CCAGGTTCCC ACTGACTCCC GACTGCAGAT CCAGCCTGGA 
GACGAGGTTG TCCAGATCAA CGAGCAGGTG GTGGTGGGAT GGCCCCGTAA GAACATGGTG 
AGGGAACTGC TGCGGGAGCC AGCCGGACTC AGCTTAGTGC TGAAGAAGAT CCCGATACCG 
GAGACCCCCC CACAGACGCC CCCTCAGGTC CTGGACTCCC CGCACCAGAG GAGCCCATCA 
CTGTCTCTGG CCCCACTGTC TCCCAGGGCC CCATCTGAAG ACGTCTTTGC CTTTGACCTG 
TCTTCAAACC CCAGTCCCGG ACCCAGCCCT GCCTGGACAG ACTCTGCCTC CCTTGGCCCT 
GAGCCCCTGC CCATCCCCCC GGAACCCCCA GCCATACTCC CAGCAGGGGT AGCAGGGACT 
CCAGGGCTCC CTGAATCCCC TGACAAGAGT CCTGTTGGTC GGAAGAAATC AAAAGGCCTG 
GCGACCCGGC TGAGCCGCCG GCGGGTGTCA TGCCGTGAGC TGGGCCGGCC GGACTGTGAC 
GGCTGGCTCC TGTTGCGAAA GGCACCGGGC GGCTTCATGG GCCCGCGCTG GCGCCGCCGC 
TGGTTTGTGC TCAAGGGACA CACGCTCTAC TGGTACCGCC AGCCCCAGGA TGAGAAGGCT 
GAGGGCCTCA TCAATGTCTC CAACTATAGT CTGGAAAGTG GACATGATCA GAAGAAGAAA 
TATGTGTTTC AGCTCACCCA TGATGTGTAC AAACCCTTCA TCTTCGCTGC TGATACCCTG 
ACAGATCTGA GCATGTGGGT GCGTCATCTC ATTACCTGCA TCTCCAAGTA CCAGTCTCCA 
GGCCGGGCCC CCCCACCCCG AGAGGAAGAC TGCTACAGTG AGACCGAAGC AGAGGACCCG 
GACGATGAGG CTGGGTCCCA CTCAGCCTCG CCCAGCCCTG CTCAAGCTGG GAGTCCCCTC 
CATGGAGACA CATCACCTGC AGCCACCCCC ACACAGCGCA GCCCACGGAC CTCCTTTGGC 
TCTCTGACAG ACAGCAGTGA AGAGGCACTG GAAGGAATGG TACGGGGGCT GAGGCAGGGT 
GGCGTGTCCC TCCTAGGCCA GCCACAGCCC CTGACCCAGG AACAGTGGCG GAGCTCTTTC 
ATGCGGCGCA ACCGAGACCC TCAGCTCAAT GAGCGAGTGC ACCGTGTGCG GGCGCTACAG 
AGCACACTCA AGGCAAAGCT GCAGGAGCTG CAGGTCCTAG AAGAAGTGCT GGGTGACCCT 
GAGCTGACAG GAGAGAAGTT CCGCCAGTGG AAGGAGCAGA ACCGGGAGCT GTACTCAGAG 
GGCCTGGGGG CCTGGGGAGT GGCACAGGCT GAAGGCAGCT CCCACATCTT GACCTCTGAC 
TCCACAGAAC AGTCCCCCCA CTCCCTGCCC TCTGACCCTG AAGAGCACTC CCATCTCTGC 
CCCCTGACCT CAGAGAGCAG CCTCCGACCT CCTGACCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CNKSR1 ( NM_006314.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_006305.2 Homo sapiens connector enhancer of kinase suppressor of Ras 1 (CNKSR1), transcript variant 1, mRNA.

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CloneID	OHu31462
Clone ID Related Accession (Same CDS sequence)	NM_006314.3 , NM_006314.2
Accession Version	NM_006314.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2142bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-08
Organism	Homo sapiens(human)
Product	connector enhancer of kinase suppressor of ras 1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA445069.1, AF100153.1 and BC011604.2. On May 16, 2008 this sequence version replaced NM_006314.1. Transcript Variant: This variant (1) encodes isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK222817.1, AB209335.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAACCGG TAGAGACCTG GACCCCCGGA AAGGTGGCAA CTTGGCTGAG AGGTCTTGAC 
GACTCCCTGC AGGACTATCC CTTTGAGGAC TGGCAGCTGC CTGGCAAGAA CCTGCTCCAG 
CTCTGCCCCC AAAGCCTCGA GGCTCTGGCT GTGCGGTCTC TGGGACACCA GGAGCTCATC 
CTGGGCGGGG TGGAACAGCT CCAGGCCCTG AGCTCCAGGC TACAGACAGA GAACCTGCAA 
AGCCTGACAG AGGGACTTCT GGGGGCAACC CATGACTTCC AGAGCATAGT CCAAGGCTGC 
CTGGGGGACT GTGCCAAGAC CCCTATTGAT GTCCTCTGTG CAGCTGTGGA GCTGTTGCAT 
GAAGCTGACG CCCTCCTCTT CTGGCTCAGC AGGTACCTCT TCTCCCACTT AAATGATTTC 
TCAGCATGCC AGGAGATCCG AGACTTGTTG GAGGAGCTGA GCCAGGTCTT GCATGAGGAT 
GGTCCAGCGG CTGAGAAGGA GGGCACAGTC CTGAGGATCT GCAGCCACGT GGCTGGGATC 
TGCCACAACA TCCTGGTCTG CTGCCCCAAG GAGCTGCTGG AACAGAAGGC CGTGCTCGAG 
CAGGTGCAGC TGGACAGTCC ATTGGGCCTA GAAATTCACA CCACCAGCAA TTGCCAGCAC 
TTTGTGTCCC AAGTGGACAC CCAGGTTCCC ACTGACTCCC GACTGCAGAT CCAGCCTGGA 
GACGAGGTTG TCCAGATCAA CGAGCAGGTG GTGGTGGGAT GGCCCCGTAA GAACATGGTG 
AGGGAACTGC TGCGGGAGCC AGCCGGACTC AGCTTAGTGC TGAAGAAGAT CCCGATACCG 
GAGACCCCCC CACAGACGCC CCCTCAGGTC CTGGACTCCC CGCACCAGAG GAGCCCATCA 
CTGTCTCTGG CCCCACTGTC TCCCAGGGCC CCATCTGAAG ACGTCTTTGC CTTTGACCTG 
TCTTCAAACC CCAGTCCCGG ACCCAGCCCT GCCTGGACAG ACTCTGCCTC CCTTGGCCCT 
GAGCCCCTGC CCATCCCCCC GGAACCCCCA GCCATACTCC CAGCAGGGGT AGCAGGGACT 
CCAGGGCTCC CTGAATCCCC TGACAAGAGT CCTGTTGGTC GGAAGAAATC AAAAGGCCTG 
GCGACCCGGC TGAGCCGCCG GCGGGTGTCA TGCCGTGAGC TGGGCCGGCC GGACTGTGAC 
GGCTGGCTCC TGTTGCGAAA GGCACCGGGC GGCTTCATGG GCCCGCGCTG GCGCCGCCGC 
TGGTTTGTGC TCAAGGGACA CACGCTCTAC TGGTACCGCC AGCCCCAGGA TGAGAAGGCT 
GAGGGCCTCA TCAATGTCTC CAACTATAGT CTGGAAAGTG GACATGATCA GAAGAAGAAA 
TATGTGTTTC AGCTCACCCA TGATGTGTAC AAACCCTTCA TCTTCGCTGC TGATACCCTG 
ACAGATCTGA GCATGTGGGT GCGTCATCTC ATTACCTGCA TCTCCAAGTA CCAGTCTCCA 
GGCCGGGCCC CCCCACCCCG AGAGGAAGAC TGCTACAGTG AGACCGAAGC AGAGGACCCG 
GACGATGAGG CTGGGTCCCA CTCAGCCTCG CCCAGCCCTG CTCAAGCTGG GAGTCCCCTC 
CATGGAGACA CATCACCTGC AGCCACCCCC ACACAGCGCA GCCCACGGAC CTCCTTTGGC 
TCTCTGACAG ACAGCAGTGA AGAGGCACTG GAAGGAATGG TACGGGGGCT GAGGCAGGGT 
GGCGTGTCCC TCCTAGGCCA GCCACAGCCC CTGACCCAGG AACAGTGGCG GAGCTCTTTC 
ATGCGGCGCA ACCGAGACCC TCAGCTCAAT GAGCGAGTGC ACCGTGTGCG GGCGCTACAG 
AGCACACTCA AGGCAAAGCT GCAGGAGCTG CAGGTCCTAG AAGAAGTGCT GGGTGACCCT 
GAGCTGACAG GAGAGAAGTT CCGCCAGTGG AAGGAGCAGA ACCGGGAGCT GTACTCAGAG 
GGCCTGGGGG CCTGGGGAGT GGCACAGGCT GAAGGCAGCT CCCACATCTT GACCTCTGAC 
TCCACAGAAC AGTCCCCCCA CTCCCTGCCC TCTGACCCTG AAGAGCACTC CCATCTCTGC 
CCCCTGACCT CAGAGAGCAG CCTCCGACCT CCTGACCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_006305.2
CDS	59..2200
Translation	MEPVETWTPGKVATWLRGLDDSLQDYPFEDWQLPGKNLLQLCPQSLEALAVRSLGHQELILGGVEQLQALSSRLQTENLQSLTEGLLGATHDFQSIVQGCLGDCAKTPIDVLCAAVELLHEADALLFWLSRYLFSHLNDFSACQEIRDLLEELSQVLHEDGPAAEKEGTVLRICSHVAGICHNILVCCPKELLEQKAVLEQVQLDSPLGLEIHTTSNCQHFVSQVDTQVPTDSRLQIQPGDEVVQINEQVVVGWPRKNMVRELLREPAGLSLVLKKIPIPETPPQTPPQVLDSPHQRSPSLSLAPLSPRAPSEDVFAFDLSSNPSPGPSPAWTDSASLGPEPLPIPPEPPAILPAGVAGTPGLPESPDKSPVGRKKSKGLATRLSRRRVSCRELGRPDCDGWLLLRKAPGGFMGPRWRRRWFVLKGHTLYWYRQPQDEKAEGLINVSNYSLESGHDQKKKYVFQLTHDVYKPFIFAADTLTDLSMWVRHLITCISKYQSPGRAPPPREEDCYSETEAEDPDDEAGSHSASPSPAQAGSPLHGDTSPAATPTQRSPRTSFGSLTDSSEEALEGMVRGLRQGGVSLLGQPQPLTQEQWRSSFMRRNRDPQLNERVHRVRALQSTLKAKLQELQVLEEVLGDPELTGEKFRQWKEQNRELYSEGLGAWGVAQAEGSSHILTSDSTEQSPHSLPSDPEEHSHLCPLTSESSLRPPDL

Target ORF information:

RefSeq Version	NM_006314.2
Organism	Homo sapiens(human)
Definition	Homo sapiens connector enhancer of kinase suppressor of Ras 1 (CNKSR1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006314.2

ORF Insert Sequence:

ATGGAACCGG TAGAGACCTG GACCCCCGGA AAGGTGGCAA CTTGGCTGAG AGGTCTTGAC 
GACTCCCTGC AGGACTATCC CTTTGAGGAC TGGCAGCTGC CTGGCAAGAA CCTGCTCCAG 
CTCTGCCCCC AAAGCCTCGA GGCTCTGGCT GTGCGGTCTC TGGGACACCA GGAGCTCATC 
CTGGGCGGGG TGGAACAGCT CCAGGCCCTG AGCTCCAGGC TACAGACAGA GAACCTGCAA 
AGCCTGACAG AGGGACTTCT GGGGGCAACC CATGACTTCC AGAGCATAGT CCAAGGCTGC 
CTGGGGGACT GTGCCAAGAC CCCTATTGAT GTCCTCTGTG CAGCTGTGGA GCTGTTGCAT 
GAAGCTGACG CCCTCCTCTT CTGGCTCAGC AGGTACCTCT TCTCCCACTT AAATGATTTC 
TCAGCATGCC AGGAGATCCG AGACTTGTTG GAGGAGCTGA GCCAGGTCTT GCATGAGGAT 
GGTCCAGCGG CTGAGAAGGA GGGCACAGTC CTGAGGATCT GCAGCCACGT GGCTGGGATC 
TGCCACAACA TCCTGGTCTG CTGCCCCAAG GAGCTGCTGG AACAGAAGGC CGTGCTCGAG 
CAGGTGCAGC TGGACAGTCC ATTGGGCCTA GAAATTCACA CCACCAGCAA TTGCCAGCAC 
TTTGTGTCCC AAGTGGACAC CCAGGTTCCC ACTGACTCCC GACTGCAGAT CCAGCCTGGA 
GACGAGGTTG TCCAGATCAA CGAGCAGGTG GTGGTGGGAT GGCCCCGTAA GAACATGGTG 
AGGGAACTGC TGCGGGAGCC AGCCGGACTC AGCTTAGTGC TGAAGAAGAT CCCGATACCG 
GAGACCCCCC CACAGACGCC CCCTCAGGTC CTGGACTCCC CGCACCAGAG GAGCCCATCA 
CTGTCTCTGG CCCCACTGTC TCCCAGGGCC CCATCTGAAG ACGTCTTTGC CTTTGACCTG 
TCTTCAAACC CCAGTCCCGG ACCCAGCCCT GCCTGGACAG ACTCTGCCTC CCTTGGCCCT 
GAGCCCCTGC CCATCCCCCC GGAACCCCCA GCCATACTCC CAGCAGGGGT AGCAGGGACT 
CCAGGGCTCC CTGAATCCCC TGACAAGAGT CCTGTTGGTC GGAAGAAATC AAAAGGCCTG 
GCGACCCGGC TGAGCCGCCG GCGGGTGTCA TGCCGTGAGC TGGGCCGGCC GGACTGTGAC 
GGCTGGCTCC TGTTGCGAAA GGCACCGGGC GGCTTCATGG GCCCGCGCTG GCGCCGCCGC 
TGGTTTGTGC TCAAGGGACA CACGCTCTAC TGGTACCGCC AGCCCCAGGA TGAGAAGGCT 
GAGGGCCTCA TCAATGTCTC CAACTATAGT CTGGAAAGTG GACATGATCA GAAGAAGAAA 
TATGTGTTTC AGCTCACCCA TGATGTGTAC AAACCCTTCA TCTTCGCTGC TGATACCCTG 
ACAGATCTGA GCATGTGGGT GCGTCATCTC ATTACCTGCA TCTCCAAGTA CCAGTCTCCA 
GGCCGGGCCC CCCCACCCCG AGAGGAAGAC TGCTACAGTG AGACCGAAGC AGAGGACCCG 
GACGATGAGG CTGGGTCCCA CTCAGCCTCG CCCAGCCCTG CTCAAGCTGG GAGTCCCCTC 
CATGGAGACA CATCACCTGC AGCCACCCCC ACACAGCGCA GCCCACGGAC CTCCTTTGGC 
TCTCTGACAG ACAGCAGTGA AGAGGCACTG GAAGGAATGG TACGGGGGCT GAGGCAGGGT 
GGCGTGTCCC TCCTAGGCCA GCCACAGCCC CTGACCCAGG AACAGTGGCG GAGCTCTTTC 
ATGCGGCGCA ACCGAGACCC TCAGCTCAAT GAGCGAGTGC ACCGTGTGCG GGCGCTACAG 
AGCACACTCA AGGCAAAGCT GCAGGAGCTG CAGGTCCTAG AAGAAGTGCT GGGTGACCCT 
GAGCTGACAG GAGAGAAGTT CCGCCAGTGG AAGGAGCAGA ACCGGGAGCT GTACTCAGAG 
GGCCTGGGGG CCTGGGGAGT GGCACAGGCT GAAGGCAGCT CCCACATCTT GACCTCTGAC 
TCCACAGAAC AGTCCCCCCA CTCCCTGCCC TCTGACCCTG AAGAGCACTC CCATCTCTGC 
CCCCTGACCT CAGAGAGCAG CCTCCGACCT CCTGACCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Expression of the scaffold connector enhancer of kinase suppressor of Ras 1 (CNKSR1) is correlated with clinical outcome in pancreatic cancer.
BMC cancer17(1)495(2017 Jul)
Quadri HS,Aiken TJ,Allgaeuer M,Moravec R,Altekruse S,Hussain SP,Miettinen MM,Hewitt SM,Rudloff U

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

AKT-dependent phosphorylation of the SAM domain induces oligomerization and activation of the scaffold protein CNK1.
Biochimica et biophysica acta1864(1)89-100(2017 01)
Fischer A,Weber W,Warscheid B,Radziwill G

Optogenetic clustering of CNK1 reveals mechanistic insights in RAF and AKT signalling controlling cell fate decisions.
Scientific reports638155(2016 Nov)
Fischer A,Warscheid B,Weber W,Radziwill G

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CNKSR1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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