EIF1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	EIF1
Entrez Gene ID	10209
Full Name	eukaryotic translation initiation factor 1
Synonyms	A121,EIF-1,EIF1A,ISO1,SUI1
General protein information	Preferred Names eukaryotic translation initiation factor 1 Names eukaryotic translation initiation factor 1 protein translation factor SUI1 homolog sui1iso1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 17 Map Location: 17q21.2

mRNA and Protein(s)

mRNA	Protein	Name
NM_005801.3	NP_005792.1	eukaryotic translation initiation factor 1
NM_005801.4	NP_005792.1	eukaryotic translation initiation factor 1

Pathways from BioSystems

Homologies

Homo sapiens (human)	EIF1	NP_005792.1
Rattus norvegicus (Norway rat)	Eif1l1	XP_003749946.1
Danio rerio (zebrafish)	eif1b	NP_955882.1
	EIF1	XP_001116333.1
Bos taurus (cattle)	EIF1	NP_001014884.1
Gallus gallus (chicken)	EIF1	NP_001161207.1
Pan troglodytes (chimpanzee)	EIF1	XP_003953067.1
Xenopus tropicalis (tropical clawed frog)	eif1	NP_001005114.1
	LOC704606	XP_002800513.1
Canis lupus familiaris (dog)	EIF1	XP_537644.2

Gene Ontology
Bibliography

Related articles in PubMed

Autoantibody response to Sui1 and its tissue-specific expression in hepatocellular carcinoma.
Zhou JW, Li Y, Yue LX, Luo CL, Chen Y, Zhang JY
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine37(2)2547-53(2016 Feb)

eIF1A augments Ago2-mediated Dicer-independent miRNA biogenesis and RNA interference.
Yi T, Arthanari H, Akabayov B, Song H, Papadopoulos E, Qi HH, Jedrychowski M, G?ttler T, Guo C, Luna RE, Gygi SP, Huang SA, Wagner G
Nature communications67194(2015 May)

Translational tolerance of mitochondrial genes to metabolic energy stress involves TISU and eIF1-eIF4GI cooperation in start codon selection.
Sinvani H, Haimov O, Svitkin Y, Sonenberg N, Tamarkin-Ben-Harush A, Viollet B, Dikstein R
Cell metabolism21(3)479-92(2015 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Anti-Sui1 could be utilized as a supplementary serological marker.
Title: Autoantibody response to Sui1 and its tissue-specific expression in hepatocellular carcinoma.

eIF1A is a novel component of the Ago2-centred RNA-induced silencing complexes (RISCs) and augments Ago2-dependent RNAi and miRNA biogenesis.
Title: eIF1A augments Ago2-mediated Dicer-independent miRNA biogenesis and RNA interference.

eEF4GI shares this activity and also interacts with eIF1.
Title: Translational tolerance of mitochondrial genes to metabolic energy stress involves TISU and eIF1-eIF4GI cooperation in start codon selection.

eIF1, eIF1A, eIF3j, and the eIF2-GTP-Met-tRNAi ternary complex stably bind to the 43 S preinitiation complex
Title: Human eukaryotic initiation factor 2 (eIF2)-GTP-Met-tRNAi ternary complex and eIF3 stabilize the 43 S preinitiation complex.

Coordinated movements of eukaryotic translation initiation factors eIF1, eIF1A, and eIF5 trigger phosphate release from eIF2 in response to start codon recognition by the ribosomal preinitiation complex
Title: Coordinated movements of eukaryotic translation initiation factors eIF1, eIF1A, and eIF5 trigger phosphate release from eIF2 in response to start codon recognition by the ribosomal preinitiation complex.

The following EIF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EIF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu13096	NM_005801.4 Latest version!	Homo sapiens eukaryotic translation initiation factor 1 (EIF1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu13096	NM_005801.3	Homo sapiens eukaryotic translation initiation factor 1 (EIF1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

EIF1 ( NM_005801.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_005792.1 Homo sapiens eukaryotic translation initiation factor 1 (EIF1), mRNA.

Price & Availability↑

CloneID	OHu13096
Clone ID Related Accession (Same CDS sequence)	NM_005801.3 , NM_005801.4
Accession Version	NM_005801.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 342bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	eukaryotic translation initiation factor 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. This record has been curated by NCBI staff in collaboration with Francesco Amaldi. The reference sequence was derived from BU165055.1, BC005118.1 and AA159721.1. On Oct 8, 2005 this sequence version replaced NM_005801.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163655.308221.1, SRR1660807.2053.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGTCCGCTA TCCAGAACCT CCACTCTTTC GACCCCTTTG CTGATGCAAG TAAGGGTGAT 
GACCTGCTTC CTGCTGGCAC TGAGGATTAT ATCCATATAA GAATTCAACA GAGAAACGGC 
AGGAAGACCC TTACTACTGT CCAAGGGATC GCTGATGATT ACGATAAAAA GAAACTAGTG 
AAGGCGTTTA AGAAAAAGTT TGCCTGCAAT GGTACTGTAA TTGAGCATCC GGAATATGGA 
GAAGTAATTC AGCTACAGGG TGACCAACGC AAGAACATAT GCCAGTTCCT CGTAGAGATT 
GGACTGGCTA AGGACGATCA GCTGAAGGTT CATGGGTTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005792.1
CDS	165..506
Translation	MSAIQNLHSFDPFADASKGDDLLPAGTEDYIHIRIQQRNGRKTLTTVQGIADDYDKKKLVKAFKKKFACNGTVIEHPEYGEVIQLQGDQRKNICQFLVEIGLAKDDQLKVHGF

Target ORF information:

RefSeq Version	NM_005801.3
Organism	Homo sapiens(human)
Definition	Homo sapiens eukaryotic translation initiation factor 1 (EIF1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005801.3

ORF Insert Sequence:

ATGTCCGCTA TCCAGAACCT CCACTCTTTC GACCCCTTTG CTGATGCAAG TAAGGGTGAT 
GACCTGCTTC CTGCTGGCAC TGAGGATTAT ATCCATATAA GAATTCAACA GAGAAACGGC 
AGGAAGACCC TTACTACTGT CCAAGGGATC GCTGATGATT ACGATAAAAA GAAACTAGTG 
AAGGCGTTTA AGAAAAAGTT TGCCTGCAAT GGTACTGTAA TTGAGCATCC GGAATATGGA 
GAAGTAATTC AGCTACAGGG TGACCAACGC AAGAACATAT GCCAGTTCCT CGTAGAGATT 
GGACTGGCTA AGGACGATCA GCTGAAGGTT CATGGGTTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EIF1 ( NM_005801.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_005792.1 Homo sapiens eukaryotic translation initiation factor 1 (EIF1), mRNA.

Price & Availability↑

CloneID	OHu13096
Clone ID Related Accession (Same CDS sequence)	NM_005801.3 , NM_005801.4
Accession Version	NM_005801.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 342bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-23
Organism	Homo sapiens(human)
Product	eukaryotic translation initiation factor 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. This record has been curated by NCBI staff in collaboration with Francesco Amaldi. The reference sequence was derived from BU165055.1, BC005118.1, AA159721.1 and AC130686.15. On Nov 22, 2018 this sequence version replaced NM_005801.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.81464.1, SRR3476690.990899.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000469257.2/ ENSP00000419449.1 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTCCGCTA TCCAGAACCT CCACTCTTTC GACCCCTTTG CTGATGCAAG TAAGGGTGAT 
GACCTGCTTC CTGCTGGCAC TGAGGATTAT ATCCATATAA GAATTCAACA GAGAAACGGC 
AGGAAGACCC TTACTACTGT CCAAGGGATC GCTGATGATT ACGATAAAAA GAAACTAGTG 
AAGGCGTTTA AGAAAAAGTT TGCCTGCAAT GGTACTGTAA TTGAGCATCC GGAATATGGA 
GAAGTAATTC AGCTACAGGG TGACCAACGC AAGAACATAT GCCAGTTCCT CGTAGAGATT 
GGACTGGCTA AGGACGATCA GCTGAAGGTT CATGGGTTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005792.1
CDS	155..496
Translation	MSAIQNLHSFDPFADASKGDDLLPAGTEDYIHIRIQQRNGRKTLTTVQGIADDYDKKKLVKAFKKKFACNGTVIEHPEYGEVIQLQGDQRKNICQFLVEIGLAKDDQLKVHGF

Target ORF information:

RefSeq Version	NM_005801.4
Organism	Homo sapiens(human)
Definition	Homo sapiens eukaryotic translation initiation factor 1 (EIF1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005801.4

ORF Insert Sequence:

ATGTCCGCTA TCCAGAACCT CCACTCTTTC GACCCCTTTG CTGATGCAAG TAAGGGTGAT 
GACCTGCTTC CTGCTGGCAC TGAGGATTAT ATCCATATAA GAATTCAACA GAGAAACGGC 
AGGAAGACCC TTACTACTGT CCAAGGGATC GCTGATGATT ACGATAAAAA GAAACTAGTG 
AAGGCGTTTA AGAAAAAGTT TGCCTGCAAT GGTACTGTAA TTGAGCATCC GGAATATGGA 
GAAGTAATTC AGCTACAGGG TGACCAACGC AAGAACATAT GCCAGTTCCT CGTAGAGATT 
GGACTGGCTA AGGACGATCA GCTGAAGGTT CATGGGTTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Autoantibody response to Sui1 and its tissue-specific expression in hepatocellular carcinoma.
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine37(2)2547-53(2016 Feb)
Zhou JW,Li Y,Yue LX,Luo CL,Chen Y,Zhang JY

eIF1A augments Ago2-mediated Dicer-independent miRNA biogenesis and RNA interference.
Nature communications67194(2015 May)
Yi T,Arthanari H,Akabayov B,Song H,Papadopoulos E,Qi HH,Jedrychowski M,G?ttler T,Guo C,Luna RE,Gygi SP,Huang SA,Wagner G

Translational tolerance of mitochondrial genes to metabolic energy stress involves TISU and eIF1-eIF4GI cooperation in start codon selection.
Cell metabolism21(3)479-92(2015 Mar)
Sinvani H,Haimov O,Svitkin Y,Sonenberg N,Tamarkin-Ben-Harush A,Viollet B,Dikstein R