ALYREF cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	ALYREF
Entrez Gene ID	10189
Full Name	Aly/REF export factor
Synonyms	ALY,ALY/REF,BEF,REF,THOC4
General protein information	Preferred Names Aly/REF export factor Names THO complex subunit 4 THO complex 4 ally of AML-1 and LEF-1 bZIP enhancing factor bZIP-enhancing factor BEF tho4 transcriptional coactivator Aly/REF
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 17 Map Location: 17q25.3
Summary	The protein encoded by this gene is a heat stable, nuclear protein and functions as a molecular chaperone. It is thought to regulate dimerization, DNA binding, and transcriptional activity of basic region-leucine zipper (bZIP) proteins. [provided by RefSeq, Jul 2008].
Disorder MIM:	604171

mRNA and Protein(s)

mRNA	Protein	Name
NM_005782.3	NP_005773.3	THO complex subunit 4
NM_005782.4	NP_005773.3	THO complex subunit 4

Pathways from BioSystems

Homologies

Bos taurus (cattle)	ALYREF	NP_001030494.1
Canis lupus familiaris (dog)	THOC4	XP_005624052.1
Homo sapiens (human)	ALYREF	NP_005773.3
Rattus norvegicus (Norway rat)	Alyref	NP_001103072.1
	THOC4	XP_001112225.2

Gene Ontology
Bibliography

Related articles in PubMed

A Map of Human Mitochondrial Protein Interactions Linked to Neurodegeneration Reveals New Mechanisms of Redox Homeostasis and NF-?B Signaling.
Malty RH, Aoki H, Kumar A, Phanse S, Amin S, Zhang Q, Minic Z, Goebels F, Musso G, Wu Z, Abou-Tok H, Meyer M, Deineko V, Kassir S, Sidhu V, Jessulat M, Scott NE, Xiong X, Vlasblom J, Prasad B, Foster LJ, Alberio T, Garavaglia B, Yu H, Bader GD, Nakamura K, Parkinson J, Babu M
Cell systems5(6)564-577.e12(2017 Dec)

Exosome cofactor hMTR4 competes with export adaptor ALYREF to ensure balanced nuclear RNA pools for degradation and export.
Fan J, Kuai B, Wu G, Wu X, Chi B, Wang L, Wang K, Shi Z, Zhang H, Chen S, He Z, Wang S, Zhou Z, Li G, Cheng H
The EMBO journal36(19)2870-2886(2017 10)

ALYREF mainly binds to the 5' and the 3' regions of the mRNA in vivo.
Shi M, Zhang H, Wu X, He Z, Wang L, Yin S, Tian B, Li G, Cheng H
Nucleic acids research45(16)9640-9653(2017 Sep)

5-methylcytosine promotes mRNA export - NSUN2 as the methyltransferase and ALYREF as an m
Yang X, Yang Y, Sun BF, Chen YS, Xu JW, Lai WY, Li A, Wang X, Bhattarai DP, Xiao W, Sun HY, Zhu Q, Ma HL, Adhikari S, Sun M, Hao YJ, Zhang B, Huang CM, Huang N, Jiang GB, Zhao YL, Wang HL, Sun YP, Yang YG
Cell research27(5)606-625(2017 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The competition between hMTR4 and ALYREF determines exosome recruitment and functions in creating balanced nuclear RNA pools for degradation and export.
Title: Exosome cofactor hMTR4 competes with export adaptor ALYREF to ensure balanced nuclear RNA pools for degradation and export.

ALYREF mainly binds to the 5' and the 3' regions of the mRNA. ALYREF is likely recruited to mRNAs by PABPN1 and the 3' processing machinery.
Title: ALYREF mainly binds to the 5' and the 3' regions of the mRNA in vivo.

Dysregulation of ALYREF-mediated mRNA export upon NSUN2 depletion could be restored by reconstitution of wild-type but not methyltransferase-defective NSUN2.
Title: 5-methylcytosine promotes mRNA export - NSUN2 as the methyltransferase and ALYREF as an m

small hairpin RNA-mediated down-regulation of TAP or Aly reduced nuclear export of HDAg-L and assembly of HDV virions. Furthermore, a peptide, TAT-HDAg-L(198-210), containing the 10-amino acid TAT peptide and HDAg-L(198-210), inhibited the interaction between HDAg-L and TAP and blocked HDV virion assembly and secretion.
Title: Cellular Nuclear Export Factors TAP and Aly Are Required for HDAg-L-mediated Assembly of Hepatitis Delta Virus.

A short conserved motif in ALYREF directs cap- and exon junction complex-dependent assembly of export complexes on spliced mRNAs.
Title: A short conserved motif in ALYREF directs cap- and EJC-dependent assembly of export complexes on spliced mRNAs.

The following ALYREF gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ALYREF cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu22888	NM_005782.4 Latest version!	Homo sapiens Aly/REF export factor (ALYREF), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu22888	NM_005782.3	Homo sapiens Aly/REF export factor (ALYREF), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ALYREF ( NM_005782.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_005773.3 Homo sapiens Aly/REF export factor (ALYREF), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu22888
Clone ID Related Accession (Same CDS sequence)	NM_005782.3 , NM_005782.4
Accession Version	NM_005782.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 795bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-21
Organism	Homo sapiens(human)
Product	THO complex subunit 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC052302.1 and BU624466.1. On Jun 2, 2009 this sequence version replaced NM_005782.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163655.79565.1, SRR1163657.501053.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2144333, SAMEA2149004 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCCGATT CCGCGCCCGC CATGGCCGAC AAAATGGACA TGTCTCTGGA CGACATCATT 
AAACTGAACC GGAGCCAGCG AGGCGGCCGG GGCGGGGGCC GGGGCCGCGG CCGGGCCGGC 
TCCCAGGGCG GCCGCGGCGG TGGGGCGCAG GCCGCCGCGC GAGTGAATCG AGGCGGCGGG 
CCCATCCGGA ACCGGCCGGC CATCGCCCGC GGCGCGGCCG GCGGAGGCGG CAGGAACCGA 
CCGGCGCCCT ACAGCAGGCC AAAACAACTT CCCGACAAGT GGCAGCACGA TCTTTTCGAC 
AGTGGCTTCG GCGGTGGTGC CGGCGTGGAG ACAGGTGGGA AACTGCTGGT GTCCAATCTG 
GATTTTGGAG TCTCAGACGC CGATATTCAG GAACTCTTTG CTGAATTTGG AACGCTGAAG 
AAGGCGGCTG TGCACTATGA TCGCTCTGGT CGCAGCTTAG GAACAGCAGA CGTGCACTTT 
GAGCGGAAGG CAGATGCCCT GAAGGCCATG AAGCAGTACA ACGGCGTCCC TCTGGATGGC 
CGCCCCATGA ACATTCAGCT TGTCACGTCA CAGATTGACG CACAGCGGAG GCCTGCACAG 
AGCGTAAACA GAGGTGGCAT GACTAGAAAC CGTGGCGCTG GAGGTTTTGG TGGTGGTGGA 
GGCACCCGGA GAGGCACCCG CGGAGGCGCC CGTGGAAGAG GCAGAGGTGC CGGCAGGAAT 
TCAAAGCAGC AGCTTTCGGC AGAGGAGCTG GATGCCCAGC TGGACGCCTA TAATGCGAGA 
ATGGACACCA GTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005773.3
CDS	7..801
Translation	MPDSAPAMADKMDMSLDDIIKLNRSQRGGRGGGRGRGRAGSQGGRGGGAQAAARVNRGGGPIRNRPAIARGAAGGGGRNRPAPYSRPKQLPDKWQHDLFDSGFGGGAGVETGGKLLVSNLDFGVSDADIQELFAEFGTLKKAAVHYDRSGRSLGTADVHFERKADALKAMKQYNGVPLDGRPMNIQLVTSQIDAQRRPAQSVNRGGMTRNRGAGGFGGGGGTRRGTRGGARGRGRGAGRNSKQQLSAEELDAQLDAYNARMDTS

Target ORF information:

RefSeq Version	NM_005782.3
Organism	Homo sapiens(human)
Definition	Homo sapiens Aly/REF export factor (ALYREF), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005782.3

ORF Insert Sequence:

ATGCCCGATT CCGCGCCCGC CATGGCCGAC AAAATGGACA TGTCTCTGGA CGACATCATT 
AAACTGAACC GGAGCCAGCG AGGCGGCCGG GGCGGGGGCC GGGGCCGCGG CCGGGCCGGC 
TCCCAGGGCG GCCGCGGCGG TGGGGCGCAG GCCGCCGCGC GAGTGAATCG AGGCGGCGGG 
CCCATCCGGA ACCGGCCGGC CATCGCCCGC GGCGCGGCCG GCGGAGGCGG CAGGAACCGA 
CCGGCGCCCT ACAGCAGGCC AAAACAACTT CCCGACAAGT GGCAGCACGA TCTTTTCGAC 
AGTGGCTTCG GCGGTGGTGC CGGCGTGGAG ACAGGTGGGA AACTGCTGGT GTCCAATCTG 
GATTTTGGAG TCTCAGACGC CGATATTCAG GAACTCTTTG CTGAATTTGG AACGCTGAAG 
AAGGCGGCTG TGCACTATGA TCGCTCTGGT CGCAGCTTAG GAACAGCAGA CGTGCACTTT 
GAGCGGAAGG CAGATGCCCT GAAGGCCATG AAGCAGTACA ACGGCGTCCC TCTGGATGGC 
CGCCCCATGA ACATTCAGCT TGTCACGTCA CAGATTGACG CACAGCGGAG GCCTGCACAG 
AGCGTAAACA GAGGTGGCAT GACTAGAAAC CGTGGCGCTG GAGGTTTTGG TGGTGGTGGA 
GGCACCCGGA GAGGCACCCG CGGAGGCGCC CGTGGAAGAG GCAGAGGTGC CGGCAGGAAT 
TCAAAGCAGC AGCTTTCGGC AGAGGAGCTG GATGCCCAGC TGGACGCCTA TAATGCGAGA 
ATGGACACCA GTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ALYREF ( NM_005782.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_005773.3 Homo sapiens Aly/REF export factor (ALYREF), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu22888
Clone ID Related Accession (Same CDS sequence)	NM_005782.3 , NM_005782.4
Accession Version	NM_005782.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 795bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-04
Organism	Homo sapiens(human)
Product	THO complex subunit 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC052302.1 and BU624466.1. On May 17, 2019 this sequence version replaced NM_005782.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163655.79565.1, SRR1163657.63300.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2144333, SAMEA2149004 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000505490.3/ ENSP00000421592.2 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCCCGATT CCGCGCCCGC CATGGCCGAC AAAATGGACA TGTCTCTGGA CGACATCATT 
AAACTGAACC GGAGCCAGCG AGGCGGCCGG GGCGGGGGCC GGGGCCGCGG CCGGGCCGGC 
TCCCAGGGCG GCCGCGGCGG TGGGGCGCAG GCCGCCGCGC GAGTGAATCG AGGCGGCGGG 
CCCATCCGGA ACCGGCCGGC CATCGCCCGC GGCGCGGCCG GCGGAGGCGG CAGGAACCGA 
CCGGCGCCCT ACAGCAGGCC AAAACAACTT CCCGACAAGT GGCAGCACGA TCTTTTCGAC 
AGTGGCTTCG GCGGTGGTGC CGGCGTGGAG ACAGGTGGGA AACTGCTGGT GTCCAATCTG 
GATTTTGGAG TCTCAGACGC CGATATTCAG GAACTCTTTG CTGAATTTGG AACGCTGAAG 
AAGGCGGCTG TGCACTATGA TCGCTCTGGT CGCAGCTTAG GAACAGCAGA CGTGCACTTT 
GAGCGGAAGG CAGATGCCCT GAAGGCCATG AAGCAGTACA ACGGCGTCCC TCTGGATGGC 
CGCCCCATGA ACATTCAGCT TGTCACGTCA CAGATTGACG CACAGCGGAG GCCTGCACAG 
AGCGTAAACA GAGGTGGCAT GACTAGAAAC CGTGGCGCTG GAGGTTTTGG TGGTGGTGGA 
GGCACCCGGA GAGGCACCCG CGGAGGCGCC CGTGGAAGAG GCAGAGGTGC CGGCAGGAAT 
TCAAAGCAGC AGCTTTCGGC AGAGGAGCTG GATGCCCAGC TGGACGCCTA TAATGCGAGA 
ATGGACACCA GTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005773.3
CDS	7..801
Translation	MPDSAPAMADKMDMSLDDIIKLNRSQRGGRGGGRGRGRAGSQGGRGGGAQAAARVNRGGGPIRNRPAIARGAAGGGGRNRPAPYSRPKQLPDKWQHDLFDSGFGGGAGVETGGKLLVSNLDFGVSDADIQELFAEFGTLKKAAVHYDRSGRSLGTADVHFERKADALKAMKQYNGVPLDGRPMNIQLVTSQIDAQRRPAQSVNRGGMTRNRGAGGFGGGGGTRRGTRGGARGRGRGAGRNSKQQLSAEELDAQLDAYNARMDTS

Target ORF information:

RefSeq Version	NM_005782.4
Organism	Homo sapiens(human)
Definition	Homo sapiens Aly/REF export factor (ALYREF), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005782.4

ORF Insert Sequence:

ATGCCCGATT CCGCGCCCGC CATGGCCGAC AAAATGGACA TGTCTCTGGA CGACATCATT 
AAACTGAACC GGAGCCAGCG AGGCGGCCGG GGCGGGGGCC GGGGCCGCGG CCGGGCCGGC 
TCCCAGGGCG GCCGCGGCGG TGGGGCGCAG GCCGCCGCGC GAGTGAATCG AGGCGGCGGG 
CCCATCCGGA ACCGGCCGGC CATCGCCCGC GGCGCGGCCG GCGGAGGCGG CAGGAACCGA 
CCGGCGCCCT ACAGCAGGCC AAAACAACTT CCCGACAAGT GGCAGCACGA TCTTTTCGAC 
AGTGGCTTCG GCGGTGGTGC CGGCGTGGAG ACAGGTGGGA AACTGCTGGT GTCCAATCTG 
GATTTTGGAG TCTCAGACGC CGATATTCAG GAACTCTTTG CTGAATTTGG AACGCTGAAG 
AAGGCGGCTG TGCACTATGA TCGCTCTGGT CGCAGCTTAG GAACAGCAGA CGTGCACTTT 
GAGCGGAAGG CAGATGCCCT GAAGGCCATG AAGCAGTACA ACGGCGTCCC TCTGGATGGC 
CGCCCCATGA ACATTCAGCT TGTCACGTCA CAGATTGACG CACAGCGGAG GCCTGCACAG 
AGCGTAAACA GAGGTGGCAT GACTAGAAAC CGTGGCGCTG GAGGTTTTGG TGGTGGTGGA 
GGCACCCGGA GAGGCACCCG CGGAGGCGCC CGTGGAAGAG GCAGAGGTGC CGGCAGGAAT 
TCAAAGCAGC AGCTTTCGGC AGAGGAGCTG GATGCCCAGC TGGACGCCTA TAATGCGAGA 
ATGGACACCA GTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A Map of Human Mitochondrial Protein Interactions Linked to Neurodegeneration Reveals New Mechanisms of Redox Homeostasis and NF-?B Signaling.
Cell systems5(6)564-577.e12(2017 Dec)
Malty RH,Aoki H,Kumar A,Phanse S,Amin S,Zhang Q,Minic Z,Goebels F,Musso G,Wu Z,Abou-Tok H,Meyer M,Deineko V,Kassir S,Sidhu V,Jessulat M,Scott NE,Xiong X,Vlasblom J,Prasad B,Foster LJ,Alberio T,Garavaglia B,Yu H,Bader GD,Nakamura K,Parkinson J,Babu M

Exosome cofactor hMTR4 competes with export adaptor ALYREF to ensure balanced nuclear RNA pools for degradation and export.
The EMBO journal36(19)2870-2886(2017 10)
Fan J,Kuai B,Wu G,Wu X,Chi B,Wang L,Wang K,Shi Z,Zhang H,Chen S,He Z,Wang S,Zhou Z,Li G,Cheng H

ALYREF mainly binds to the 5' and the 3' regions of the mRNA in vivo.
Nucleic acids research45(16)9640-9653(2017 Sep)
Shi M,Zhang H,Wu X,He Z,Wang L,Yin S,Tian B,Li G,Cheng H

5-methylcytosine promotes mRNA export - NSUN2 as the methyltransferase and ALYREF as an m
Cell research27(5)606-625(2017 May)
Yang X,Yang Y,Sun BF,Chen YS,Xu JW,Lai WY,Li A,Wang X,Bhattarai DP,Xiao W,Sun HY,Zhu Q,Ma HL,Adhikari S,Sun M,Hao YJ,Zhang B,Huang CM,Huang N,Jiang GB,Zhao YL,Wang HL,Sun YP,Yang YG

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ALYREF cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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