CHST4 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	CHST4
Entrez Gene ID	10164
Full Name	carbohydrate sulfotransferase 4
Synonyms	GST3,GlcNAc6ST2,HECGLCNAC6ST,LSST
General protein information	Preferred Names carbohydrate sulfotransferase 4 Names carbohydrate sulfotransferase 4 GST-3 HEC-GlcNAc6ST L-selectin ligand sulfotransferase N-acetylglucosamine 6-O-sulfotransferase 2 carbohydrate (N-acetylglucosamine 6-O) sulfotransferase 4 galactose/N-acetylglucosamine/N-acetylgalactosamine 6-O-sulfotransferase 3 galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 3 glcNAc6ST-2 gn6st-2 high endothelial cells N-acetylglucosamine 6-O-sulfotransferase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 16 Map Location: 16q22.2
Summary	This gene encodes an N-acetylglucosamine 6-O sulfotransferase. The encoded enzyme transfers sulfate from 3'phosphoadenosine 5'phospho-sulfate to the 6-hydroxyl group of N-acetylglucosamine on glycoproteins. This protein is localized to the Golgi and is involved in the modification of glycan structures on ligands of the lymphocyte homing receptor L-selectin. Alternate splicing in the 5' UTR results in multiple transcript variants that encode the same protein. [provided by RefSeq, Oct 2009].

mRNA and Protein(s)

mRNA	Protein	Name
NM_005769.2	NP_005760.1	carbohydrate sulfotransferase 4
NM_001166395.2	NP_001159867.1	carbohydrate sulfotransferase 4
NM_001166395.1	NP_001159867.1	carbohydrate sulfotransferase 4

Pathways from BioSystems

Homologies

	CHST4	XP_001106318.1
Canis lupus familiaris (dog)	CHST4	XP_005620798.1
Bos taurus (cattle)	CHST4	NP_001069613.1
Rattus norvegicus (Norway rat)	Chst4	NP_001100897.1
Gallus gallus (chicken)	CHST4	XP_004944421.1
Mus musculus (house mouse)	Chst4	NP_036128.3
Xenopus tropicalis (tropical clawed frog)	LOC100496630	XP_004914310.1
Pan troglodytes (chimpanzee)	CHST4	XP_001171156.1
Homo sapiens (human)	CHST4	NP_005760.1

Gene Ontology
Bibliography

Related articles in PubMed

Apical membrane expression of distinct sulfated glycans represents a novel marker of cholangiolocellular carcinoma.
Hoshino H, Ohta M, Ito M, Uchimura K, Sakai Y, Uehara T, Low S, Fukushima M, Kobayashi M
Laboratory investigation; a journal of technical methods and pathology96(12)1246-1255(2016 Dec)

Detecting O-GlcNAc using in vitro sulfation.
Wu ZL, Robey MT, Tatge T, Lin C, Leymarie N, Zou Y, Zaia J
Glycobiology24(8)740-7(2014 Aug)

N-Acetylglucosamine 6-O-sulfotransferase-2 as a tumor marker for uterine cervical and corpus cancer.
Seko A, Kataoka F, Aoki D, Sakamoto M, Nakamura T, Hatae M, Yonezawa S, Yamashita K
Glycoconjugate journal26(8)1065-73(2009 Nov)

Sulfated L-selectin ligands as a therapeutic target in chronic inflammation.
Uchimura K, Rosen SD
Trends in immunology27(12)559-65(2006 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data suggest that GlcNAc6ST-2 (CHST4) is critical for biosynthesis of MECA-79 sulfated glycans in apical membranes of small-sized intrahepatic bile ducts and of cholangiolocellular carcinoma (CoCC) cells; MUC1 protein decorated with GlcNAc6ST-2-dependent MECA-79 sulfated glycans may serve as a useful CoCC marker.
Title: Apical membrane expression of distinct sulfated glycans represents a novel marker of cholangiolocellular carcinoma.

A method for O-GlcNAc detection using in vitro sulfation with two N-acetylglucosamine (GlcNAc)-specific sulfotransferases, carbohydrate sulfotransferase 2 and carbohydrate sulfotransferase 4.
Title: Detecting O-GlcNAc using in vitro sulfation.

GlcNAc6ST2 could therefore be a good serological marker for detecting early-stage uterine cervical and corpus cancers.
Title: N-Acetylglucosamine 6-O-sulfotransferase-2 as a tumor marker for uterine cervical and corpus cancer.

Observational study of gene-disease association. (HuGE Navigator)
Title: Association study between single-nucleotide polymorphisms in 199 drug-related genes and commonly measured quantitative traits of 752 healthy Japanese subjects.

N-acetylgluosamine-6-O-sulfotransferase is overexpressed in a subgroup of paediatric precursor-B acute lymphoblastic leukaemia
Title: High level expression of N-acetylgluosamine-6-O-sulfotransferase is characteristic of a subgroup of paediatric precursor-B acute lymphoblastic leukaemia.

The following CHST4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CHST4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu22500	NM_005769.2 Latest version!	Homo sapiens carbohydrate sulfotransferase 4 (CHST4), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu22500	NM_001166395.2 Latest version!	Homo sapiens carbohydrate sulfotransferase 4 (CHST4), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
View Old Accession Versions >>
OHu22500	NM_001166395.1	Homo sapiens carbohydrate sulfotransferase 4 (CHST4), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CHST4 ( NM_005769.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_005760.1 Homo sapiens carbohydrate sulfotransferase 4 (CHST4), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu22500
Clone ID Related Accession (Same CDS sequence)	NM_005769.2 , NM_001166395.2 , NM_001166395.1
Accession Version	NM_005769.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1161bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Homo sapiens(human)
Product	carbohydrate sulfotransferase 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA496702.1, AF131235.1, AC010547.9 and BC035282.1. This sequence is a reference standard in the RefSeqGene project. On Oct 29, 2009 this sequence version replaced NM_005769.1. Transcript Variant: This variant (1) and variant 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF131235.1, AK026635.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA2146982 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTACTGC CTAAAAAAAT GAAGCTCCTG CTGTTTCTGG TTTCCCAGAT GGCCATCTTG 
GCTCTATTCT TCCACATGTA CAGCCACAAC ATCAGCTCCC TGTCTATGAA GGCACAGCCC 
GAGCGCATGC ACGTGCTGGT TCTGTCTTCC TGGCGCTCTG GCTCTTCTTT TGTGGGGCAG 
CTTTTTGGGC AGCACCCAGA TGTTTTCTAC CTGATGGAGC CCGCCTGGCA CGTGTGGATG 
ACCTTCAAGC AGAGCACCGC CTGGATGCTG CACATGGCTG TGCGGGATCT GATACGGGCC 
GTCTTCTTGT GCGACATGAG CGTCTTTGAT GCCTACATGG AACCTGGTCC CCGGAGACAG 
TCCAGCCTCT TTCAGTGGGA GAACAGCCGG GCCCTGTGTT CTGCACCTGC CTGTGACATC 
ATCCCACAAG ATGAAATCAT CCCCCGGGCT CACTGCAGGC TCCTGTGCAG TCAACAGCCC 
TTTGAGGTGG TGGAGAAGGC CTGCCGCTCC TACAGCCACG TGGTGCTCAA GGAGGTGCGC 
TTCTTCAACC TGCAGTCCCT CTACCCGCTG CTGAAAGACC CCTCCCTCAA CCTGCATATC 
GTGCACCTGG TCCGGGACCC CCGGGCCGTG TTCCGTTCCC GAGAACGCAC AAAGGGAGAT 
CTCATGATTG ACAGTCGCAT TGTGATGGGG CAGCATGAGC AAAAACTCAA GAAGGAGGAC 
CAACCCTACT ATGTGATGCA GGTCATCTGC CAAAGCCAGC TGGAGATCTA CAAGACCATC 
CAGTCCTTGC CCAAGGCCCT GCAGGAACGC TACCTGCTTG TGCGCTATGA GGACCTGGCT 
CGAGCCCCTG TGGCCCAGAC TTCCCGAATG TATGAATTCG TGGGATTGGA ATTCTTGCCC 
CATCTTCAGA CCTGGGTGCA TAACATCACC CGAGGCAAGG GCATGGGTGA CCACGCTTTC 
CACACAAATG CCAGGGATGC CCTTAATGTC TCCCAGGCTT GGCGCTGGTC TTTGCCCTAT 
GAAAAGGTTT CTCGACTTCA GAAAGCCTGT GGCGATGCCA TGAATTTGCT GGGCTACCGC 
CACGTCAGAT CTGAACAAGA ACAGAGAAAC CTGTTGCTGG ATCTTCTGTC TACCTGGACT 
GTCCCTGAGC AAATCCACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005760.1
CDS	212..1372
Translation	MLLPKKMKLLLFLVSQMAILALFFHMYSHNISSLSMKAQPERMHVLVLSSWRSGSSFVGQLFGQHPDVFYLMEPAWHVWMTFKQSTAWMLHMAVRDLIRAVFLCDMSVFDAYMEPGPRRQSSLFQWENSRALCSAPACDIIPQDEIIPRAHCRLLCSQQPFEVVEKACRSYSHVVLKEVRFFNLQSLYPLLKDPSLNLHIVHLVRDPRAVFRSRERTKGDLMIDSRIVMGQHEQKLKKEDQPYYVMQVICQSQLEIYKTIQSLPKALQERYLLVRYEDLARAPVAQTSRMYEFVGLEFLPHLQTWVHNITRGKGMGDHAFHTNARDALNVSQAWRWSLPYEKVSRLQKACGDAMNLLGYRHVRSEQEQRNLLLDLLSTWTVPEQIH

Target ORF information:

RefSeq Version	NM_005769.2
Organism	Homo sapiens(human)
Definition	Homo sapiens carbohydrate sulfotransferase 4 (CHST4), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005769.2

ORF Insert Sequence:

ATGCTACTGC CTAAAAAAAT GAAGCTCCTG CTGTTTCTGG TTTCCCAGAT GGCCATCTTG 
GCTCTATTCT TCCACATGTA CAGCCACAAC ATCAGCTCCC TGTCTATGAA GGCACAGCCC 
GAGCGCATGC ACGTGCTGGT TCTGTCTTCC TGGCGCTCTG GCTCTTCTTT TGTGGGGCAG 
CTTTTTGGGC AGCACCCAGA TGTTTTCTAC CTGATGGAGC CCGCCTGGCA CGTGTGGATG 
ACCTTCAAGC AGAGCACCGC CTGGATGCTG CACATGGCTG TGCGGGATCT GATACGGGCC 
GTCTTCTTGT GCGACATGAG CGTCTTTGAT GCCTACATGG AACCTGGTCC CCGGAGACAG 
TCCAGCCTCT TTCAGTGGGA GAACAGCCGG GCCCTGTGTT CTGCACCTGC CTGTGACATC 
ATCCCACAAG ATGAAATCAT CCCCCGGGCT CACTGCAGGC TCCTGTGCAG TCAACAGCCC 
TTTGAGGTGG TGGAGAAGGC CTGCCGCTCC TACAGCCACG TGGTGCTCAA GGAGGTGCGC 
TTCTTCAACC TGCAGTCCCT CTACCCGCTG CTGAAAGACC CCTCCCTCAA CCTGCATATC 
GTGCACCTGG TCCGGGACCC CCGGGCCGTG TTCCGTTCCC GAGAACGCAC AAAGGGAGAT 
CTCATGATTG ACAGTCGCAT TGTGATGGGG CAGCATGAGC AAAAACTCAA GAAGGAGGAC 
CAACCCTACT ATGTGATGCA GGTCATCTGC CAAAGCCAGC TGGAGATCTA CAAGACCATC 
CAGTCCTTGC CCAAGGCCCT GCAGGAACGC TACCTGCTTG TGCGCTATGA GGACCTGGCT 
CGAGCCCCTG TGGCCCAGAC TTCCCGAATG TATGAATTCG TGGGATTGGA ATTCTTGCCC 
CATCTTCAGA CCTGGGTGCA TAACATCACC CGAGGCAAGG GCATGGGTGA CCACGCTTTC 
CACACAAATG CCAGGGATGC CCTTAATGTC TCCCAGGCTT GGCGCTGGTC TTTGCCCTAT 
GAAAAGGTTT CTCGACTTCA GAAAGCCTGT GGCGATGCCA TGAATTTGCT GGGCTACCGC 
CACGTCAGAT CTGAACAAGA ACAGAGAAAC CTGTTGCTGG ATCTTCTGTC TACCTGGACT 
GTCCCTGAGC AAATCCACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHST4 ( NM_001166395.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001159867.1 Homo sapiens carbohydrate sulfotransferase 4 (CHST4), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu22500
Clone ID Related Accession (Same CDS sequence)	NM_005769.2 , NM_001166395.2 , NM_001166395.1
Accession Version	NM_001166395.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1161bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-28
Organism	Homo sapiens(human)
Product	carbohydrate sulfotransferase 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA045171.1, BC035282.1 and AC010547.9. On Jun 2, 2019 this sequence version replaced NM_001166395.1. Transcript Variant: This variant (2) has an alternate 5' UTR exon, as compared to variant 1. Variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC035282.1, AF280088.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCTACTGC CTAAAAAAAT GAAGCTCCTG CTGTTTCTGG TTTCCCAGAT GGCCATCTTG 
GCTCTATTCT TCCACATGTA CAGCCACAAC ATCAGCTCCC TGTCTATGAA GGCACAGCCC 
GAGCGCATGC ACGTGCTGGT TCTGTCTTCC TGGCGCTCTG GCTCTTCTTT TGTGGGGCAG 
CTTTTTGGGC AGCACCCAGA TGTTTTCTAC CTGATGGAGC CCGCCTGGCA CGTGTGGATG 
ACCTTCAAGC AGAGCACCGC CTGGATGCTG CACATGGCTG TGCGGGATCT GATACGGGCC 
GTCTTCTTGT GCGACATGAG CGTCTTTGAT GCCTACATGG AACCTGGTCC CCGGAGACAG 
TCCAGCCTCT TTCAGTGGGA GAACAGCCGG GCCCTGTGTT CTGCACCTGC CTGTGACATC 
ATCCCACAAG ATGAAATCAT CCCCCGGGCT CACTGCAGGC TCCTGTGCAG TCAACAGCCC 
TTTGAGGTGG TGGAGAAGGC CTGCCGCTCC TACAGCCACG TGGTGCTCAA GGAGGTGCGC 
TTCTTCAACC TGCAGTCCCT CTACCCGCTG CTGAAAGACC CCTCCCTCAA CCTGCATATC 
GTGCACCTGG TCCGGGACCC CCGGGCCGTG TTCCGTTCCC GAGAACGCAC AAAGGGAGAT 
CTCATGATTG ACAGTCGCAT TGTGATGGGG CAGCATGAGC AAAAACTCAA GAAGGAGGAC 
CAACCCTACT ATGTGATGCA GGTCATCTGC CAAAGCCAGC TGGAGATCTA CAAGACCATC 
CAGTCCTTGC CCAAGGCCCT GCAGGAACGC TACCTGCTTG TGCGCTATGA GGACCTGGCT 
CGAGCCCCTG TGGCCCAGAC TTCCCGAATG TATGAATTCG TGGGATTGGA ATTCTTGCCC 
CATCTTCAGA CCTGGGTGCA TAACATCACC CGAGGCAAGG GCATGGGTGA CCACGCTTTC 
CACACAAATG CCAGGGATGC CCTTAATGTC TCCCAGGCTT GGCGCTGGTC TTTGCCCTAT 
GAAAAGGTTT CTCGACTTCA GAAAGCCTGT GGCGATGCCA TGAATTTGCT GGGCTACCGC 
CACGTCAGAT CTGAACAAGA ACAGAGAAAC CTGTTGCTGG ATCTTCTGTC TACCTGGACT 
GTCCCTGAGC AAATCCACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001159867.1
CDS	89..1249
Translation	MLLPKKMKLLLFLVSQMAILALFFHMYSHNISSLSMKAQPERMHVLVLSSWRSGSSFVGQLFGQHPDVFYLMEPAWHVWMTFKQSTAWMLHMAVRDLIRAVFLCDMSVFDAYMEPGPRRQSSLFQWENSRALCSAPACDIIPQDEIIPRAHCRLLCSQQPFEVVEKACRSYSHVVLKEVRFFNLQSLYPLLKDPSLNLHIVHLVRDPRAVFRSRERTKGDLMIDSRIVMGQHEQKLKKEDQPYYVMQVICQSQLEIYKTIQSLPKALQERYLLVRYEDLARAPVAQTSRMYEFVGLEFLPHLQTWVHNITRGKGMGDHAFHTNARDALNVSQAWRWSLPYEKVSRLQKACGDAMNLLGYRHVRSEQEQRNLLLDLLSTWTVPEQIH

Target ORF information:

RefSeq Version	NM_001166395.2
Organism	Homo sapiens(human)
Definition	Homo sapiens carbohydrate sulfotransferase 4 (CHST4), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001166395.2

ORF Insert Sequence:

ATGCTACTGC CTAAAAAAAT GAAGCTCCTG CTGTTTCTGG TTTCCCAGAT GGCCATCTTG 
GCTCTATTCT TCCACATGTA CAGCCACAAC ATCAGCTCCC TGTCTATGAA GGCACAGCCC 
GAGCGCATGC ACGTGCTGGT TCTGTCTTCC TGGCGCTCTG GCTCTTCTTT TGTGGGGCAG 
CTTTTTGGGC AGCACCCAGA TGTTTTCTAC CTGATGGAGC CCGCCTGGCA CGTGTGGATG 
ACCTTCAAGC AGAGCACCGC CTGGATGCTG CACATGGCTG TGCGGGATCT GATACGGGCC 
GTCTTCTTGT GCGACATGAG CGTCTTTGAT GCCTACATGG AACCTGGTCC CCGGAGACAG 
TCCAGCCTCT TTCAGTGGGA GAACAGCCGG GCCCTGTGTT CTGCACCTGC CTGTGACATC 
ATCCCACAAG ATGAAATCAT CCCCCGGGCT CACTGCAGGC TCCTGTGCAG TCAACAGCCC 
TTTGAGGTGG TGGAGAAGGC CTGCCGCTCC TACAGCCACG TGGTGCTCAA GGAGGTGCGC 
TTCTTCAACC TGCAGTCCCT CTACCCGCTG CTGAAAGACC CCTCCCTCAA CCTGCATATC 
GTGCACCTGG TCCGGGACCC CCGGGCCGTG TTCCGTTCCC GAGAACGCAC AAAGGGAGAT 
CTCATGATTG ACAGTCGCAT TGTGATGGGG CAGCATGAGC AAAAACTCAA GAAGGAGGAC 
CAACCCTACT ATGTGATGCA GGTCATCTGC CAAAGCCAGC TGGAGATCTA CAAGACCATC 
CAGTCCTTGC CCAAGGCCCT GCAGGAACGC TACCTGCTTG TGCGCTATGA GGACCTGGCT 
CGAGCCCCTG TGGCCCAGAC TTCCCGAATG TATGAATTCG TGGGATTGGA ATTCTTGCCC 
CATCTTCAGA CCTGGGTGCA TAACATCACC CGAGGCAAGG GCATGGGTGA CCACGCTTTC 
CACACAAATG CCAGGGATGC CCTTAATGTC TCCCAGGCTT GGCGCTGGTC TTTGCCCTAT 
GAAAAGGTTT CTCGACTTCA GAAAGCCTGT GGCGATGCCA TGAATTTGCT GGGCTACCGC 
CACGTCAGAT CTGAACAAGA ACAGAGAAAC CTGTTGCTGG ATCTTCTGTC TACCTGGACT 
GTCCCTGAGC AAATCCACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHST4 ( NM_001166395.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001159867.1 Homo sapiens carbohydrate sulfotransferase 4 (CHST4), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu22500
Clone ID Related Accession (Same CDS sequence)	NM_005769.2 , NM_001166395.2 , NM_001166395.1
Accession Version	NM_001166395.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1161bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-11
Organism	Homo sapiens(human)
Product	carbohydrate sulfotransferase 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA045171.1 and BC035282.1. Transcript Variant: This variant (2) has an alternate 5' UTR exon, as compared to variant 1. Variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC035282.1, AF280088.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTACTGC CTAAAAAAAT GAAGCTCCTG CTGTTTCTGG TTTCCCAGAT GGCCATCTTG 
GCTCTATTCT TCCACATGTA CAGCCACAAC ATCAGCTCCC TGTCTATGAA GGCACAGCCC 
GAGCGCATGC ACGTGCTGGT TCTGTCTTCC TGGCGCTCTG GCTCTTCTTT TGTGGGGCAG 
CTTTTTGGGC AGCACCCAGA TGTTTTCTAC CTGATGGAGC CCGCCTGGCA CGTGTGGATG 
ACCTTCAAGC AGAGCACCGC CTGGATGCTG CACATGGCTG TGCGGGATCT GATACGGGCC 
GTCTTCTTGT GCGACATGAG CGTCTTTGAT GCCTACATGG AACCTGGTCC CCGGAGACAG 
TCCAGCCTCT TTCAGTGGGA GAACAGCCGG GCCCTGTGTT CTGCACCTGC CTGTGACATC 
ATCCCACAAG ATGAAATCAT CCCCCGGGCT CACTGCAGGC TCCTGTGCAG TCAACAGCCC 
TTTGAGGTGG TGGAGAAGGC CTGCCGCTCC TACAGCCACG TGGTGCTCAA GGAGGTGCGC 
TTCTTCAACC TGCAGTCCCT CTACCCGCTG CTGAAAGACC CCTCCCTCAA CCTGCATATC 
GTGCACCTGG TCCGGGACCC CCGGGCCGTG TTCCGTTCCC GAGAACGCAC AAAGGGAGAT 
CTCATGATTG ACAGTCGCAT TGTGATGGGG CAGCATGAGC AAAAACTCAA GAAGGAGGAC 
CAACCCTACT ATGTGATGCA GGTCATCTGC CAAAGCCAGC TGGAGATCTA CAAGACCATC 
CAGTCCTTGC CCAAGGCCCT GCAGGAACGC TACCTGCTTG TGCGCTATGA GGACCTGGCT 
CGAGCCCCTG TGGCCCAGAC TTCCCGAATG TATGAATTCG TGGGATTGGA ATTCTTGCCC 
CATCTTCAGA CCTGGGTGCA TAACATCACC CGAGGCAAGG GCATGGGTGA CCACGCTTTC 
CACACAAATG CCAGGGATGC CCTTAATGTC TCCCAGGCTT GGCGCTGGTC TTTGCCCTAT 
GAAAAGGTTT CTCGACTTCA GAAAGCCTGT GGCGATGCCA TGAATTTGCT GGGCTACCGC 
CACGTCAGAT CTGAACAAGA ACAGAGAAAC CTGTTGCTGG ATCTTCTGTC TACCTGGACT 
GTCCCTGAGC AAATCCACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001159867.1
CDS	264..1424
Translation	MLLPKKMKLLLFLVSQMAILALFFHMYSHNISSLSMKAQPERMHVLVLSSWRSGSSFVGQLFGQHPDVFYLMEPAWHVWMTFKQSTAWMLHMAVRDLIRAVFLCDMSVFDAYMEPGPRRQSSLFQWENSRALCSAPACDIIPQDEIIPRAHCRLLCSQQPFEVVEKACRSYSHVVLKEVRFFNLQSLYPLLKDPSLNLHIVHLVRDPRAVFRSRERTKGDLMIDSRIVMGQHEQKLKKEDQPYYVMQVICQSQLEIYKTIQSLPKALQERYLLVRYEDLARAPVAQTSRMYEFVGLEFLPHLQTWVHNITRGKGMGDHAFHTNARDALNVSQAWRWSLPYEKVSRLQKACGDAMNLLGYRHVRSEQEQRNLLLDLLSTWTVPEQIH

Target ORF information:

RefSeq Version	NM_001166395.1
Organism	Homo sapiens(human)
Definition	Homo sapiens carbohydrate sulfotransferase 4 (CHST4), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001166395.1

ORF Insert Sequence:

ATGCTACTGC CTAAAAAAAT GAAGCTCCTG CTGTTTCTGG TTTCCCAGAT GGCCATCTTG 
GCTCTATTCT TCCACATGTA CAGCCACAAC ATCAGCTCCC TGTCTATGAA GGCACAGCCC 
GAGCGCATGC ACGTGCTGGT TCTGTCTTCC TGGCGCTCTG GCTCTTCTTT TGTGGGGCAG 
CTTTTTGGGC AGCACCCAGA TGTTTTCTAC CTGATGGAGC CCGCCTGGCA CGTGTGGATG 
ACCTTCAAGC AGAGCACCGC CTGGATGCTG CACATGGCTG TGCGGGATCT GATACGGGCC 
GTCTTCTTGT GCGACATGAG CGTCTTTGAT GCCTACATGG AACCTGGTCC CCGGAGACAG 
TCCAGCCTCT TTCAGTGGGA GAACAGCCGG GCCCTGTGTT CTGCACCTGC CTGTGACATC 
ATCCCACAAG ATGAAATCAT CCCCCGGGCT CACTGCAGGC TCCTGTGCAG TCAACAGCCC 
TTTGAGGTGG TGGAGAAGGC CTGCCGCTCC TACAGCCACG TGGTGCTCAA GGAGGTGCGC 
TTCTTCAACC TGCAGTCCCT CTACCCGCTG CTGAAAGACC CCTCCCTCAA CCTGCATATC 
GTGCACCTGG TCCGGGACCC CCGGGCCGTG TTCCGTTCCC GAGAACGCAC AAAGGGAGAT 
CTCATGATTG ACAGTCGCAT TGTGATGGGG CAGCATGAGC AAAAACTCAA GAAGGAGGAC 
CAACCCTACT ATGTGATGCA GGTCATCTGC CAAAGCCAGC TGGAGATCTA CAAGACCATC 
CAGTCCTTGC CCAAGGCCCT GCAGGAACGC TACCTGCTTG TGCGCTATGA GGACCTGGCT 
CGAGCCCCTG TGGCCCAGAC TTCCCGAATG TATGAATTCG TGGGATTGGA ATTCTTGCCC 
CATCTTCAGA CCTGGGTGCA TAACATCACC CGAGGCAAGG GCATGGGTGA CCACGCTTTC 
CACACAAATG CCAGGGATGC CCTTAATGTC TCCCAGGCTT GGCGCTGGTC TTTGCCCTAT 
GAAAAGGTTT CTCGACTTCA GAAAGCCTGT GGCGATGCCA TGAATTTGCT GGGCTACCGC 
CACGTCAGAT CTGAACAAGA ACAGAGAAAC CTGTTGCTGG ATCTTCTGTC TACCTGGACT 
GTCCCTGAGC AAATCCACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Apical membrane expression of distinct sulfated glycans represents a novel marker of cholangiolocellular carcinoma.
Laboratory investigation; a journal of technical methods and pathology96(12)1246-1255(2016 Dec)
Hoshino H,Ohta M,Ito M,Uchimura K,Sakai Y,Uehara T,Low S,Fukushima M,Kobayashi M

Detecting O-GlcNAc using in vitro sulfation.
Glycobiology24(8)740-7(2014 Aug)
Wu ZL,Robey MT,Tatge T,Lin C,Leymarie N,Zou Y,Zaia J

N-Acetylglucosamine 6-O-sulfotransferase-2 as a tumor marker for uterine cervical and corpus cancer.
Glycoconjugate journal26(8)1065-73(2009 Nov)
Seko A,Kataoka F,Aoki D,Sakamoto M,Nakamura T,Hatae M,Yonezawa S,Yamashita K

Sulfated L-selectin ligands as a therapeutic target in chronic inflammation.
Trends in immunology27(12)559-65(2006 Dec)
Uchimura K,Rosen SD

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CHST4 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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