RASA4 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	RASA4
Entrez Gene ID	10156
Full Name	RAS p21 protein activator 4
Synonyms	CAPRI,GAPL
General protein information	Preferred Names RAS p21 protein activator 4 Names ras GTPase-activating protein 4 Ca2+-promoted Ras inactivator calcium-promoted Ras inactivator rasGAP-activating-like protein 2
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 7 Map Location: 7q22.1
Summary	This gene encodes a member of the GAP1 family of GTPase-activating proteins that suppresses the Ras/mitogen-activated protein kinase pathway in response to Ca(2+). Stimuli that increase intracellular Ca(2+) levels result in the translocation of this protein to the plasma membrane, where it activates Ras GTPase activity. Consequently, Ras is converted from the active GTP-bound state to the inactive GDP-bound state and no longer activates downstream pathways that regulate gene expression, cell growth, and differentiation. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008].
Disorder MIM:	607943

mRNA and Protein(s)

mRNA	Protein	Name
NM_001079877.2	NP_001073346.2	ras GTPase-activating protein 4 isoform 2
XM_017011662.1	XP_016867151.1	ras GTPase-activating protein 4 isoform X2
NM_006989.6	NP_008920.5	ras GTPase-activating protein 4 isoform 1
XM_017011662.2	XP_016867151.1	ras GTPase-activating protein 4 isoform X2
XM_011515723.1	XP_011514025.1	ras GTPase-activating protein 4 isoform X3
XM_017011661.1	XP_016867150.1	ras GTPase-activating protein 4 isoform X1
NM_006989.5	NP_008920.5	ras GTPase-activating protein 4 isoform 1
XM_017011661.2	XP_016867150.1	ras GTPase-activating protein 4 isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	RASA4	XP_546937.4
Danio rerio (zebrafish)	rasa4	NP_001092921.1
Rattus norvegicus (Norway rat)	Rasa4	XP_002727999.1
Gallus gallus (chicken)	RASA4	XP_415761.4
Homo sapiens (human)	RASA4	NP_008920.5
Pan troglodytes (chimpanzee)	LOC742087	XP_003318744.1
Bos taurus (cattle)	RASA4	XP_003587887.2
Mus musculus (house mouse)	Rasa4	NP_598675.2
Xenopus tropicalis (tropical clawed frog)	LOC100488193	XP_002933873.1

Gene Ontology
Bibliography

Related articles in PubMed

RASA4 undergoes DNA hypermethylation in resistant juvenile myelomonocytic leukemia.
Poetsch AR, Lipka DB, Witte T, Claus R, N?llke P, Zucknick M, Olk-Batz C, Fluhr S, Dworzak M, De Moerloose B, Star? J, Zecca M, Hasle H, Schmugge M, van den Heuvel-Eibrink MM, Locatelli F, Niemeyer CM, Flotho C, Plass C
Epigenetics9(9)1252-60(2014 Sep)

Ca2+-dependent monomer and dimer formation switches CAPRI Protein between Ras GTPase-activating protein (GAP) and RapGAP activities.
Dai Y, Walker SA, de Vet E, Cook S, Welch HC, Lockyer PJ
The Journal of biological chemistry286(22)19905-16(2011 Jun)

CAPRI and RASAL impose different modes of information processing on Ras due to contrasting temporal filtering of Ca2+.
Liu Q, Walker SA, Gao D, Taylor JA, Dai YF, Arkell RS, Bootman MD, Roderick HL, Cullen PJ, Lockyer PJ
The Journal of cell biology170(2)183-90(2005 Jul)

Phospholipase Cgamma activates Ras on the Golgi apparatus by means of RasGRP1.
Bivona TG, P?rez De Castro I, Ahearn IM, Grana TM, Chiu VK, Lockyer PJ, Cullen PJ, Pellicer A, Cox AD, Philips MR
Nature424(6949)694-8(2003 Aug)

Distinct phosphoinositide binding specificity of the GAP1 family proteins: characterization of the pleckstrin homology domains of MRASAL and KIAA0538.
Minagawa T, Fukuda M, Mikoshiba K
Biochemical and biophysical research communications288(1)87-90(2001 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

RASA4 isoform 2 promoter methylation correlated with clinical parameters predicting poor prognosis (older age, elevated fetal hemoglobin), with higher risk of relapse after hematopoietic stem cell transplantation, and with PTPN11 mutation.
Title: RASA4 undergoes DNA hypermethylation in resistant juvenile myelomonocytic leukemia.

Ca2+-dependent monomer and dimer formation switches CAPRI Protein between Ras GTPase-activating protein (GAP) and RapGAP activities
Title: Ca2+-dependent monomer and dimer formation switches CAPRI Protein between Ras GTPase-activating protein (GAP) and RapGAP activities.

CAPRI seems to low-pass filter the Ca2+ signal, converting different intensities of stimulation into different durations of Ras activity.
Title: CAPRI and RASAL impose different modes of information processing on Ras due to contrasting temporal filtering of Ca2+.

CAPRI (Ca2+-promoted Ras inactivator) a Ca2+-dependent Ras GTPase-activating protein (Ras GAP). Switches off the Ras-MAPK pathway following G protein-coupled receptor stimulated intracellular Ca2+ elevation that recruits CAPRI to the plasma membrane.
Title: CAPRI regulates Ca(2+)-dependent inactivation of the Ras-MAPK pathway.

The following RASA4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RASA4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu01710	NM_001079877.2 Latest version!	Homo sapiens RAS p21 protein activator 4 (RASA4), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$531.30 $759.00
OHu63736	XM_011515723.1 Latest version!	Homo sapiens RAS p21 protein activator 4 (RASA4), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu01164	NM_006989.6 Latest version!	Homo sapiens RAS p21 protein activator 4 (RASA4), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu96998	XM_017011661.2 Latest version!	Homo sapiens RAS p21 protein activator 4 (RASA4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OHu96999	XM_017011662.2 Latest version!	Homo sapiens RAS p21 protein activator 4 (RASA4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OHu96999	XM_017011662.1	Homo sapiens RAS p21 protein activator 4 (RASA4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu01164	NM_006989.5	Homo sapiens RAS p21 protein activator 4 (RASA4), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$265.30 $379.00
OHu96998	XM_017011661.1	Homo sapiens RAS p21 protein activator 4 (RASA4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RASA4 ( NM_006989.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_008920.5 Homo sapiens RAS p21 protein activator 4 (RASA4), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu01164
Clone ID Related Accession (Same CDS sequence)	NM_006989.6 , NM_006989.5
Accession Version	NM_006989.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2412bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-30
Organism	Homo sapiens(human)
Product	ras GTPase-activating protein 4 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC105052.3 and AC093668.4. On Jun 30, 2010 this sequence version replaced NM_006989.4. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AB011110.2, BC110873.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCCAAGC GCAGCTCGCT GTACATCCGC ATCGTGGAGG GGAAGAACCT TCCCGCCAAG 
GACATCACTG GCAGCAGCGA CCCCTACTGC ATCGTGAAGG TGGACAATGA GCCCATCATC 
AGGACAGCCA CAGTGTGGAA GACCCTGTGC CCCTTCTGGG GTGAGGAGTA CCAAGTGCAC 
CTGCCGCCCA CCTTCCACGC TGTGGCTTTC TATGTCATGG ATGAGGATGC CCTCAGCCGG 
GACGACGTTA TCGGAAAGGT CTGCCTTACA AGGGACACCA TAGCCTCTCA CCCTAAGGGT 
TTCAGCGGGT GGGCCCACCT GACGGAGGTC GACCCCGATG AGGAGGTGCA GGGCGAGATC 
CACCTGCGGC TGGAAGTGTG GCCAGGGGCC CGGGCCTGCC GGCTACGCTG CTCTGTGCTG 
GAGGCCAGGG ATCTGGCCCC AAAGGACCGC AATGGCACAT CTGACCCCTT CGTCCGAGTG 
CGCTACAAGG GCCGGACACG GGAGACCTCG ATCGTGAAGA AGTCATGCTA CCCACGCTGG 
AATGAGACGT TTGAATTTGA GCTGCAGGAG GGGGCCATGG AGGCGCTGTG CGTGGAGGCC 
TGGGACTGGG ACCTTGTCAG CCGAAACGAC TTCCTGGGCA AAGTGGTGAT TGATGTCCAG 
AGACTGCGGG TGGTGCAGCA GGAGGAGGGC TGGTTCCGGC TGCAGCCCGA CCAGTCCAAG 
AGCCGGCGGC ATGACGAGGG CAACCTGGGC TCCTTGCAGC TGGAGGTGCG GCTGCGGGAC 
GAGACGGTGC TGCCCTCCAG CTACTACCAG CCACTGGTGC ACCTGCTGTG CCACGAGGTC 
AAGCTGGGCA TGCAGGGCCC AGGGCAGCTG ATCCCACTCA TCGAGGAGAC AACCAGCACC 
GAGTGTCGCC AGGACGTGGC CACGAACCTG CTCAAGCTCT TCCTGGGGCA GGGGCTGGCC 
AAGGACTTCC TGGACCTGCT CTTCCAGCTG GAGCTGAGTC GCACCAGTGA GACCAACACC 
CTGTTCCGGA GCAACTCTCT GGCCTCAAAG TCCATGGAGT CTTTTCTGAA GGTGGCCGGG 
ATGCAGTACC TGCACGGCGT CCTGGGCCCC ATCATCAACA AGGTGTTTGA GGAGAAGAAG 
TACGTGGAGC TGGACCCCAG CAAAGTGGAA GTTAAGGATG TAGGGTGCTC CGGGCTGCAC 
CGCCCGCAGA CCGAGGCCGA GGTGCTGGAG CAGAGCGCGC AGACGCTGCG CGCCCACCTG 
GGGGCCCTGC TGAGCGCGCT CAGCCGCTCG GTTCGCGCGT GCCCCGCCGT GGTGCGCGCC 
ACCTTCCGCC AGCTCTTCCG GCGCGTGCGC GAGCGCTTCC CCGGCGCCCA GCACGAGAAT 
GTACCGTTCA TCGCCGTCAC CAGCTTCCTG TGCCTGCGCT TCTTCTCTCC CGCCATCATG 
TCGCCCAAGC TCTTCCACCT GCGGGAGCGC CACGCGGACG CCCGCACCAG CCGCACCCTG 
CTCCTGTTGG CCAAGGCAGT CCAGAACGTG GGCAACATGG ACACGCCGGC TTCCAGGGCC 
AAGGAGGCTT GGATGGAGCC GCTGCAGCCC ACCGTGCGCC AGGGCGTGGC GCAGCTGAAG 
GACTTCATCA CCAAGCTCGT GGACATCGAG GAGAAGGACG AGCTGGACCT GCAGCGGACG 
CTGAGTTTGC AGGCGCCACC TGTGAAGGAG GGGCCACTCT TCATCCACAG GACCAAGGGC 
AAGGGCCCCC TCATGTCCTC CTCCTTCAAG AAGCTCTACT TCTCCCTCAC TACCGAGGCC 
CTCAGCTTCG CGAAGACGCC CAGCTCCAAG AAAAGCGCCC TCATCAAGTT AGCCAACATC 
CGGGCAGCGG AAAAGGTTGA GGAAAAGAGC TTTGGCGGCT CGCACGTCAT GCAGGTCATC 
TACACGGACG ACGCCGGCAG GCCCCAGACT GCCTACCTGC AGTGCAAGTG TGTGAATGAG 
CTTAACCAGT GGCTGTCTGC GCTGCGGAAG GTGAGCATCA ACAACACCGG ACTGCTGGGC 
TCCTACCACC CTGGCGTCTT CCGTGGGGAC AAGTGGAGCT GCTGCCACCA AAAAGAGAAG 
ACAGGTCAGG GCTGCGATAA GACCCGGTCA CGGGTGACCC TGCAGGAGTG GAATGACCCT 
CTTGACCATG ACCTTGAGGC CCAGCTCATC TACCGGCACC TGCTGGGCGT GGAGGCCATG 
CTGTGGGAGA GGCACCGGGA GCTGAGCGGG GGCGCAGAGG CAGGCACGGT GCCCACGAGC 
CCTGGCAAAG TCCCCGAGGA CTCATTGGCC CGGCTGCTCC GGGTGCTGCA GGACCTCCGC 
GAGGCCCATA GCTCCAGCCC GGCCGGCTCC CCACCCTCAG AGCCCAACTG CCTCCTGGAG 
CTGCAGACGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_008920.5
CDS	70..2481
Translation	MAKRSSLYIRIVEGKNLPAKDITGSSDPYCIVKVDNEPIIRTATVWKTLCPFWGEEYQVHLPPTFHAVAFYVMDEDALSRDDVIGKVCLTRDTIASHPKGFSGWAHLTEVDPDEEVQGEIHLRLEVWPGARACRLRCSVLEARDLAPKDRNGTSDPFVRVRYKGRTRETSIVKKSCYPRWNETFEFELQEGAMEALCVEAWDWDLVSRNDFLGKVVIDVQRLRVVQQEEGWFRLQPDQSKSRRHDEGNLGSLQLEVRLRDETVLPSSYYQPLVHLLCHEVKLGMQGPGQLIPLIEETTSTECRQDVATNLLKLFLGQGLAKDFLDLLFQLELSRTSETNTLFRSNSLASKSMESFLKVAGMQYLHGVLGPIINKVFEEKKYVELDPSKVEVKDVGCSGLHRPQTEAEVLEQSAQTLRAHLGALLSALSRSVRACPAVVRATFRQLFRRVRERFPGAQHENVPFIAVTSFLCLRFFSPAIMSPKLFHLRERHADARTSRTLLLLAKAVQNVGNMDTPASRAKEAWMEPLQPTVRQGVAQLKDFITKLVDIEEKDELDLQRTLSLQAPPVKEGPLFIHRTKGKGPLMSSSFKKLYFSLTTEALSFAKTPSSKKSALIKLANIRAAEKVEEKSFGGSHVMQVIYTDDAGRPQTAYLQCKCVNELNQWLSALRKVSINNTGLLGSYHPGVFRGDKWSCCHQKEKTGQGCDKTRSRVTLQEWNDPLDHDLEAQLIYRHLLGVEAMLWERHRELSGGAEAGTVPTSPGKVPEDSLARLLRVLQDLREAHSSSPAGSPPSEPNCLLELQT

Target ORF information:

RefSeq Version	NM_006989.5
Organism	Homo sapiens(human)
Definition	Homo sapiens RAS p21 protein activator 4 (RASA4), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006989.5

ORF Insert Sequence:

ATGGCCAAGC GCAGCTCGCT GTACATCCGC ATCGTGGAGG GGAAGAACCT TCCCGCCAAG 
GACATCACTG GCAGCAGCGA CCCCTACTGC ATCGTGAAGG TGGACAATGA GCCCATCATC 
AGGACAGCCA CAGTGTGGAA GACCCTGTGC CCCTTCTGGG GTGAGGAGTA CCAAGTGCAC 
CTGCCGCCCA CCTTCCACGC TGTGGCTTTC TATGTCATGG ATGAGGATGC CCTCAGCCGG 
GACGACGTTA TCGGAAAGGT CTGCCTTACA AGGGACACCA TAGCCTCTCA CCCTAAGGGT 
TTCAGCGGGT GGGCCCACCT GACGGAGGTC GACCCCGATG AGGAGGTGCA GGGCGAGATC 
CACCTGCGGC TGGAAGTGTG GCCAGGGGCC CGGGCCTGCC GGCTACGCTG CTCTGTGCTG 
GAGGCCAGGG ATCTGGCCCC AAAGGACCGC AATGGCACAT CTGACCCCTT CGTCCGAGTG 
CGCTACAAGG GCCGGACACG GGAGACCTCG ATCGTGAAGA AGTCATGCTA CCCACGCTGG 
AATGAGACGT TTGAATTTGA GCTGCAGGAG GGGGCCATGG AGGCGCTGTG CGTGGAGGCC 
TGGGACTGGG ACCTTGTCAG CCGAAACGAC TTCCTGGGCA AAGTGGTGAT TGATGTCCAG 
AGACTGCGGG TGGTGCAGCA GGAGGAGGGC TGGTTCCGGC TGCAGCCCGA CCAGTCCAAG 
AGCCGGCGGC ATGACGAGGG CAACCTGGGC TCCTTGCAGC TGGAGGTGCG GCTGCGGGAC 
GAGACGGTGC TGCCCTCCAG CTACTACCAG CCACTGGTGC ACCTGCTGTG CCACGAGGTC 
AAGCTGGGCA TGCAGGGCCC AGGGCAGCTG ATCCCACTCA TCGAGGAGAC AACCAGCACC 
GAGTGTCGCC AGGACGTGGC CACGAACCTG CTCAAGCTCT TCCTGGGGCA GGGGCTGGCC 
AAGGACTTCC TGGACCTGCT CTTCCAGCTG GAGCTGAGTC GCACCAGTGA GACCAACACC 
CTGTTCCGGA GCAACTCTCT GGCCTCAAAG TCCATGGAGT CTTTTCTGAA GGTGGCCGGG 
ATGCAGTACC TGCACGGCGT CCTGGGCCCC ATCATCAACA AGGTGTTTGA GGAGAAGAAG 
TACGTGGAGC TGGACCCCAG CAAAGTGGAA GTTAAGGATG TAGGGTGCTC CGGGCTGCAC 
CGCCCGCAGA CCGAGGCCGA GGTGCTGGAG CAGAGCGCGC AGACGCTGCG CGCCCACCTG 
GGGGCCCTGC TGAGCGCGCT CAGCCGCTCG GTTCGCGCGT GCCCCGCCGT GGTGCGCGCC 
ACCTTCCGCC AGCTCTTCCG GCGCGTGCGC GAGCGCTTCC CCGGCGCCCA GCACGAGAAT 
GTACCGTTCA TCGCCGTCAC CAGCTTCCTG TGCCTGCGCT TCTTCTCTCC CGCCATCATG 
TCGCCCAAGC TCTTCCACCT GCGGGAGCGC CACGCGGACG CCCGCACCAG CCGCACCCTG 
CTCCTGTTGG CCAAGGCAGT CCAGAACGTG GGCAACATGG ACACGCCGGC TTCCAGGGCC 
AAGGAGGCTT GGATGGAGCC GCTGCAGCCC ACCGTGCGCC AGGGCGTGGC GCAGCTGAAG 
GACTTCATCA CCAAGCTCGT GGACATCGAG GAGAAGGACG AGCTGGACCT GCAGCGGACG 
CTGAGTTTGC AGGCGCCACC TGTGAAGGAG GGGCCACTCT TCATCCACAG GACCAAGGGC 
AAGGGCCCCC TCATGTCCTC CTCCTTCAAG AAGCTCTACT TCTCCCTCAC TACCGAGGCC 
CTCAGCTTCG CGAAGACGCC CAGCTCCAAG AAAAGCGCCC TCATCAAGTT AGCCAACATC 
CGGGCAGCGG AAAAGGTTGA GGAAAAGAGC TTTGGCGGCT CGCACGTCAT GCAGGTCATC 
TACACGGACG ACGCCGGCAG GCCCCAGACT GCCTACCTGC AGTGCAAGTG TGTGAATGAG 
CTTAACCAGT GGCTGTCTGC GCTGCGGAAG GTGAGCATCA ACAACACCGG ACTGCTGGGC 
TCCTACCACC CTGGCGTCTT CCGTGGGGAC AAGTGGAGCT GCTGCCACCA AAAAGAGAAG 
ACAGGTCAGG GCTGCGATAA GACCCGGTCA CGGGTGACCC TGCAGGAGTG GAATGACCCT 
CTTGACCATG ACCTTGAGGC CCAGCTCATC TACCGGCACC TGCTGGGCGT GGAGGCCATG 
CTGTGGGAGA GGCACCGGGA GCTGAGCGGG GGCGCAGAGG CAGGCACGGT GCCCACGAGC 
CCTGGCAAAG TCCCCGAGGA CTCATTGGCC CGGCTGCTCC GGGTGCTGCA GGACCTCCGC 
GAGGCCCATA GCTCCAGCCC GGCCGGCTCC CCACCCTCAG AGCCCAACTG CCTCCTGGAG 
CTGCAGACGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASA4 ( NM_001079877.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001073346.2 Homo sapiens RAS p21 protein activator 4 (RASA4), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu01710
Clone ID Related Accession (Same CDS sequence)	NM_001079877.2
Accession Version	NM_001079877.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2274bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-02
Organism	Homo sapiens(human)
Product	ras GTPase-activating protein 4 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC105052.3 and AC093668.4. On Jun 30, 2010 this sequence version replaced NM_001079877.1. Transcript Variant: This variant (2) lacks an alternate in-frame exon, compared to variant 1, resulting in a shorter protein (isoform 2), compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AK026441.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCAAGC GCAGCTCGCT GTACATCCGC ATCGTGGAGG GGAAGAACCT TCCCGCCAAG 
GACATCACTG GCAGCAGCGA CCCCTACTGC ATCGTGAAGG TGGACAATGA GCCCATCATC 
AGGACAGCCA CAGTGTGGAA GACCCTGTGC CCCTTCTGGG GTGAGGAGTA CCAAGTGCAC 
CTGCCGCCCA CCTTCCACGC TGTGGCTTTC TATGTCATGG ATGAGGATGC CCTCAGCCGG 
GACGACGTTA TCGGAAAGGT CTGCCTTACA AGGGACACCA TAGCCTCTCA CCCTAAGGGT 
TTCAGCGGGT GGGCCCACCT GACGGAGGTC GACCCCGATG AGGAGGTGCA GGGCGAGATC 
CACCTGCGGC TGGAAGTGTG GCCAGGGGCC CGGGCCTGCC GGCTACGCTG CTCTGTGCTG 
GAGGCCAGGG ATCTGGCCCC AAAGGACCGC AATGGCACAT CTGACCCCTT CGTCCGAGTG 
CGCTACAAGG GCCGGACACG GGAGACCTCG ATCGTGAAGA AGTCATGCTA CCCACGCTGG 
AATGAGACGT TTGAATTTGA GCTGCAGGAG GGGGCCATGG AGGCGCTGTG CGTGGAGGCC 
TGGGACTGGG ACCTTGTCAG CCGAAACGAC TTCCTGGGCA AAGTGGTGAT TGATGTCCAG 
AGACTGCGGG TGGTGCAGCA GGAGGAGGGC TGGTTCCGGC TGCAGCCCGA CCAGTCCAAG 
AGCCGGCGGC ATGACGAGGG CAACCTGGGC TCCTTGCAGC TGGAGGTGCG GCTGCGGGAC 
GAGACGGTGC TGCCCTCCAG CTACTACCAG CCACTGGTGC ACCTGCTGTG CCACGAGGTC 
AAGCTGGGCA TGCAGGGCCC AGGGCAGCTG ATCCCACTCA TCGAGGAGAC AACCAGCACC 
GAGTGTCGCC AGGACGTGGC CACGAACCTG CTCAAGCTCT TCCTGGGGCA GGGGCTGGCC 
AAGGACTTCC TGGACCTGCT CTTCCAGCTG GAGCTGAGTC GCACCAGTGA GACCAACACC 
CTGTTCCGGA GCAACTCTCT GGCCTCAAAG TCCATGGAGT CTTTTCTGAA GGTGGCCGGG 
ATGCAGTACC TGCACGGCGT CCTGGGCCCC ATCATCAACA AGGTGTTTGA GGAGAAGAAG 
TACGTGGAGC TGGACCCCAG CAAAGTGGAA GTTAAGGATG TAGGGTGCTC CGGGCTGCAC 
CGCCCGCAGA CCGAGGCCGA GGTGCTGGAG CAGAGCGCGC AGACGCTGCG CGCCCACCTG 
GGGGCCCTGC TGAGCGCGCT CAGCCGCTCG GTTCGCGCGT GCCCCGCCGT GGTGCGCGCC 
ACCTTCCGCC AGCTCTTCCG GCGCGTGCGC GAGCGCTTCC CCGGCGCCCA GCACGAGAAT 
GTACCGTTCA TCGCCGTCAC CAGCTTCCTG TGCCTGCGCT TCTTCTCTCC CGCCATCATG 
TCGCCCAAGC TCTTCCACCT GCGGGAGCGC CACGCGGACG CCCGCACCAG CCGCACCCTG 
CTCCTGTTGG CCAAGGCAGT CCAGAACGTG GGCAACATGG ACACGCCGGC TTCCAGGGCC 
AAGGAGGCTT GGATGGAGCC GCTGCAGCCC ACCGTGCGCC AGGGCGTGGC GCAGCTGAAG 
GACTTCATCA CCAAGCTCGT GGACATCGAG GAGAAGGACG AGCTGGACCT GCAGCGGACG 
CTGAGTTTGC AGGCGCCACC TGTGAAGGAG GGGCCACTCT TCATCCACAG GACCAAGGGC 
AAGGGCCCCC TCATGTCCTC CTCCTTCAAG AAGCTCTACT TCTCCCTCAC TACCGAGGCC 
CTCAGCTTCG CGAAGACGCC CAGCTCCAAG TGTGTGAATG AGCTTAACCA GTGGCTGTCT 
GCGCTGCGGA AGGTGAGCAT CAACAACACC GGACTGCTGG GCTCCTACCA CCCTGGCGTC 
TTCCGTGGGG ACAAGTGGAG CTGCTGCCAC CAAAAAGAGA AGACAGGTCA GGGCTGCGAT 
AAGACCCGGT CACGGGTGAC CCTGCAGGAG TGGAATGACC CTCTTGACCA TGACCTTGAG 
GCCCAGCTCA TCTACCGGCA CCTGCTGGGC GTGGAGGCCA TGCTGTGGGA GAGGCACCGG 
GAGCTGAGCG GGGGCGCAGA GGCAGGCACG GTGCCCACGA GCCCTGGCAA AGTCCCCGAG 
GACTCATTGG CCCGGCTGCT CCGGGTGCTG CAGGACCTCC GCGAGGCCCA TAGCTCCAGC 
CCGGCCGGCT CCCCACCCTC AGAGCCCAAC TGCCTCCTGG AGCTGCAGAC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001073346.2
CDS	70..2343
Translation	MAKRSSLYIRIVEGKNLPAKDITGSSDPYCIVKVDNEPIIRTATVWKTLCPFWGEEYQVHLPPTFHAVAFYVMDEDALSRDDVIGKVCLTRDTIASHPKGFSGWAHLTEVDPDEEVQGEIHLRLEVWPGARACRLRCSVLEARDLAPKDRNGTSDPFVRVRYKGRTRETSIVKKSCYPRWNETFEFELQEGAMEALCVEAWDWDLVSRNDFLGKVVIDVQRLRVVQQEEGWFRLQPDQSKSRRHDEGNLGSLQLEVRLRDETVLPSSYYQPLVHLLCHEVKLGMQGPGQLIPLIEETTSTECRQDVATNLLKLFLGQGLAKDFLDLLFQLELSRTSETNTLFRSNSLASKSMESFLKVAGMQYLHGVLGPIINKVFEEKKYVELDPSKVEVKDVGCSGLHRPQTEAEVLEQSAQTLRAHLGALLSALSRSVRACPAVVRATFRQLFRRVRERFPGAQHENVPFIAVTSFLCLRFFSPAIMSPKLFHLRERHADARTSRTLLLLAKAVQNVGNMDTPASRAKEAWMEPLQPTVRQGVAQLKDFITKLVDIEEKDELDLQRTLSLQAPPVKEGPLFIHRTKGKGPLMSSSFKKLYFSLTTEALSFAKTPSSKCVNELNQWLSALRKVSINNTGLLGSYHPGVFRGDKWSCCHQKEKTGQGCDKTRSRVTLQEWNDPLDHDLEAQLIYRHLLGVEAMLWERHRELSGGAEAGTVPTSPGKVPEDSLARLLRVLQDLREAHSSSPAGSPPSEPNCLLELQT

Target ORF information:

RefSeq Version	NM_001079877.2
Organism	Homo sapiens(human)
Definition	Homo sapiens RAS p21 protein activator 4 (RASA4), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001079877.2

ORF Insert Sequence:

ATGGCCAAGC GCAGCTCGCT GTACATCCGC ATCGTGGAGG GGAAGAACCT TCCCGCCAAG 
GACATCACTG GCAGCAGCGA CCCCTACTGC ATCGTGAAGG TGGACAATGA GCCCATCATC 
AGGACAGCCA CAGTGTGGAA GACCCTGTGC CCCTTCTGGG GTGAGGAGTA CCAAGTGCAC 
CTGCCGCCCA CCTTCCACGC TGTGGCTTTC TATGTCATGG ATGAGGATGC CCTCAGCCGG 
GACGACGTTA TCGGAAAGGT CTGCCTTACA AGGGACACCA TAGCCTCTCA CCCTAAGGGT 
TTCAGCGGGT GGGCCCACCT GACGGAGGTC GACCCCGATG AGGAGGTGCA GGGCGAGATC 
CACCTGCGGC TGGAAGTGTG GCCAGGGGCC CGGGCCTGCC GGCTACGCTG CTCTGTGCTG 
GAGGCCAGGG ATCTGGCCCC AAAGGACCGC AATGGCACAT CTGACCCCTT CGTCCGAGTG 
CGCTACAAGG GCCGGACACG GGAGACCTCG ATCGTGAAGA AGTCATGCTA CCCACGCTGG 
AATGAGACGT TTGAATTTGA GCTGCAGGAG GGGGCCATGG AGGCGCTGTG CGTGGAGGCC 
TGGGACTGGG ACCTTGTCAG CCGAAACGAC TTCCTGGGCA AAGTGGTGAT TGATGTCCAG 
AGACTGCGGG TGGTGCAGCA GGAGGAGGGC TGGTTCCGGC TGCAGCCCGA CCAGTCCAAG 
AGCCGGCGGC ATGACGAGGG CAACCTGGGC TCCTTGCAGC TGGAGGTGCG GCTGCGGGAC 
GAGACGGTGC TGCCCTCCAG CTACTACCAG CCACTGGTGC ACCTGCTGTG CCACGAGGTC 
AAGCTGGGCA TGCAGGGCCC AGGGCAGCTG ATCCCACTCA TCGAGGAGAC AACCAGCACC 
GAGTGTCGCC AGGACGTGGC CACGAACCTG CTCAAGCTCT TCCTGGGGCA GGGGCTGGCC 
AAGGACTTCC TGGACCTGCT CTTCCAGCTG GAGCTGAGTC GCACCAGTGA GACCAACACC 
CTGTTCCGGA GCAACTCTCT GGCCTCAAAG TCCATGGAGT CTTTTCTGAA GGTGGCCGGG 
ATGCAGTACC TGCACGGCGT CCTGGGCCCC ATCATCAACA AGGTGTTTGA GGAGAAGAAG 
TACGTGGAGC TGGACCCCAG CAAAGTGGAA GTTAAGGATG TAGGGTGCTC CGGGCTGCAC 
CGCCCGCAGA CCGAGGCCGA GGTGCTGGAG CAGAGCGCGC AGACGCTGCG CGCCCACCTG 
GGGGCCCTGC TGAGCGCGCT CAGCCGCTCG GTTCGCGCGT GCCCCGCCGT GGTGCGCGCC 
ACCTTCCGCC AGCTCTTCCG GCGCGTGCGC GAGCGCTTCC CCGGCGCCCA GCACGAGAAT 
GTACCGTTCA TCGCCGTCAC CAGCTTCCTG TGCCTGCGCT TCTTCTCTCC CGCCATCATG 
TCGCCCAAGC TCTTCCACCT GCGGGAGCGC CACGCGGACG CCCGCACCAG CCGCACCCTG 
CTCCTGTTGG CCAAGGCAGT CCAGAACGTG GGCAACATGG ACACGCCGGC TTCCAGGGCC 
AAGGAGGCTT GGATGGAGCC GCTGCAGCCC ACCGTGCGCC AGGGCGTGGC GCAGCTGAAG 
GACTTCATCA CCAAGCTCGT GGACATCGAG GAGAAGGACG AGCTGGACCT GCAGCGGACG 
CTGAGTTTGC AGGCGCCACC TGTGAAGGAG GGGCCACTCT TCATCCACAG GACCAAGGGC 
AAGGGCCCCC TCATGTCCTC CTCCTTCAAG AAGCTCTACT TCTCCCTCAC TACCGAGGCC 
CTCAGCTTCG CGAAGACGCC CAGCTCCAAG TGTGTGAATG AGCTTAACCA GTGGCTGTCT 
GCGCTGCGGA AGGTGAGCAT CAACAACACC GGACTGCTGG GCTCCTACCA CCCTGGCGTC 
TTCCGTGGGG ACAAGTGGAG CTGCTGCCAC CAAAAAGAGA AGACAGGTCA GGGCTGCGAT 
AAGACCCGGT CACGGGTGAC CCTGCAGGAG TGGAATGACC CTCTTGACCA TGACCTTGAG 
GCCCAGCTCA TCTACCGGCA CCTGCTGGGC GTGGAGGCCA TGCTGTGGGA GAGGCACCGG 
GAGCTGAGCG GGGGCGCAGA GGCAGGCACG GTGCCCACGA GCCCTGGCAA AGTCCCCGAG 
GACTCATTGG CCCGGCTGCT CCGGGTGCTG CAGGACCTCC GCGAGGCCCA TAGCTCCAGC 
CCGGCCGGCT CCCCACCCTC AGAGCCCAAC TGCCTCCTGG AGCTGCAGAC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASA4 ( XM_011515723.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011514025.1 Homo sapiens RAS p21 protein activator 4 (RASA4), transcript variant X6, mRNA.

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CloneID	OHu63736
Clone ID Related Accession (Same CDS sequence)	XM_011515723.1
Accession Version	XM_011515723.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2049bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ras GTPase-activating protein 4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGC GCAGCTCGCT GTACATCCGC ATCGTGGAGG GGAAGAACCT TCCCGCCAAG 
GACATCACTG GCAGCAGCGA CCCCTACTGC ATCGTGAAGG TGGACAATGA GCCCATCATC 
AGGACAGCCA CAGTGTGGAA GACCCTGTGC CCCTTCTGGG GTGAGGAGTA CCAAGTGCAC 
CTGCCGCCCA CCTTCCACGC TGTGGCTTTC TATGTCATGG ATGAGGATGC CCTCAGCCGG 
GACGACGTTA TCGGAAAGGT CTGCCTTACA AGGGACACCA TAGCCTCTCA CCCTAAGGGT 
TTCAGCGGGT GGGCCCACCT GACGGAGGTC GACCCCGATG AGGAGGTGCA GGGCGAGATC 
CACCTGCGGC TGGAAGTGTG GCCAGGGGCC CGGGCCTGCC GGCTACGCTG CTCTGTGCTG 
GAGGCCAGGG ATCTGGCCCC AAAGGACCGC AATGGCACAT CTGACCCCTT CGTCCGAGTG 
CGCTACAAGG GCCGGACACG GGAGACCTCG ATCGTGAAGA AGTCATGCTA CCCACGCTGG 
AATGAGACGT TTGAATTTGA GCTGCAGGAG GGGGCCATGG AGGCGCTGTG CGTGGAGGCC 
TGGGACTGGG ACCTTGTCAG CCGAAACGAC TTCCTGGGCA AAGTGGTGAT TGATGTCCAG 
AGACTGCGGG TGGTGCAGCA GGAGGAGGGC TGGTTCCGGC TGCAGCCCGA CCAGTCCAAG 
AGCCGGCGGC ATGACGAGGG CAACCTGGGC TCCTTGCAGC TGGAGGTGCG GCTGCGGGAC 
GAGACGGTGC TGCCCTCCAG CTACTACCAG CCACTGGTGC ACCTGCTGTG CCACGAGGTC 
AAGCTGGGCA TGCAGGGCCC AGGGCAGCTG ATCCCACTCA TCGAGGAGAC AACCAGCACC 
GAGTGTCGCC AGGACGTGGC CACGAACCTG CTCAAGCTCT TCCTGGGGCA GGGGCTGGCC 
AAGGACTTCC TGGACCTGCT CTTCCAGCTG GAGCTGAGTC GCACCAGTGA GACCAACACC 
CTGTTCCGGA GCAACTCTCT GGCCTCAAAG TCCATGGAGT CTTTTCTGAA GGTGGCCGGG 
ATGCAGTACC TGCACGGCGT CCTGGGCCCC ATCATCAACA AGGTGTTTGA GGAGAAGAAG 
TACGTGGAGC TGGACCCCAG CAAAGTGGAA GTTAAGGATG TAGGGTGCTC CGGGCTGCAC 
CGCCCGCAGA CCGAGGCCGA GGTGCTGGAG CAGAGCGCGC AGACGCTGCG CGCCCACCTG 
GGGGCCCTGC TGAGCGCGCT CAGCCGCTCG GTTCGCGCGT GCCCCGCCGT GGTGCGCGCC 
ACCTTCCGCC AGCTCTTCCG GCGCGTGCGC GAGCGCTTCC CCGGCGCCCA GCACGAGAAT 
GTACCGTTCA TCGCCGTCAC CAGCTTCCTG TGCCTGCGCT TCTTCTCTCC CGCCATCATG 
TCGCCCAAGC TCTTCCACCT GCGGGAGCGC CACGCGGACG CCCGCACCAG CCGCACCCTG 
CTCCTGTTGG CCAAGGCAGT CCAGAACGTG GGCAACATGG ACACGCCGGC TTCCAGGGCC 
AAGGAGGCTT GGATGGAGCC GCTGCAGCCC ACCGTGCGCC AGGGCGTGGC GCAGCTGAAG 
GACTTCATCA CCAAGCTCGT GGACATCGAG GAGAAGGACG AGCTGGACCT GCAGCGGACG 
CTGAGTTTGC AGGCGCCACC TGTGAAGGAG GGGCCACTCT TCATCCACAG GACCAAGGGC 
AAGGGCCCCC TCATGTCCTC CTCCTTCAAG AAGCTCTACT TCTCCCTCAC TACCGAGGCC 
CTCAGCTTCG CGAAGACGCC CAGCTCCAAG GAGTTCCCTT TTGTGTCAGG GTCCAGAGCC 
ACTGGGAAAA GTAGTGATAT CAGGGCCACC AAATATATTT GGCGGGTTCT GGAATACCTA 
CGAGCGTGGC CAAGGGGCCA GCGGCGGCTG AAATCCAGCC CACACCAGCC TCCTGGGCTC 
CTACCATCCC GGTGCCTTCC GTGGGGACAA GTGGAGCTGC TGCCACCAAA AAGACGAGAC 
AGTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011514025.1
CDS	69..2117
Translation	MAKRSSLYIRIVEGKNLPAKDITGSSDPYCIVKVDNEPIIRTATVWKTLCPFWGEEYQVHLPPTFHAVAFYVMDEDALSRDDVIGKVCLTRDTIASHPKGFSGWAHLTEVDPDEEVQGEIHLRLEVWPGARACRLRCSVLEARDLAPKDRNGTSDPFVRVRYKGRTRETSIVKKSCYPRWNETFEFELQEGAMEALCVEAWDWDLVSRNDFLGKVVIDVQRLRVVQQEEGWFRLQPDQSKSRRHDEGNLGSLQLEVRLRDETVLPSSYYQPLVHLLCHEVKLGMQGPGQLIPLIEETTSTECRQDVATNLLKLFLGQGLAKDFLDLLFQLELSRTSETNTLFRSNSLASKSMESFLKVAGMQYLHGVLGPIINKVFEEKKYVELDPSKVEVKDVGCSGLHRPQTEAEVLEQSAQTLRAHLGALLSALSRSVRACPAVVRATFRQLFRRVRERFPGAQHENVPFIAVTSFLCLRFFSPAIMSPKLFHLRERHADARTSRTLLLLAKAVQNVGNMDTPASRAKEAWMEPLQPTVRQGVAQLKDFITKLVDIEEKDELDLQRTLSLQAPPVKEGPLFIHRTKGKGPLMSSSFKKLYFSLTTEALSFAKTPSSKEFPFVSGSRATGKSSDIRATKYIWRVLEYLRAWPRGQRRLKSSPHQPPGLLPSRCLPWGQVELLPPKRRDSV

Target ORF information:

RefSeq Version	XM_011515723.1
Organism	Homo sapiens(human)
Definition	Homo sapiens RAS p21 protein activator 4 (RASA4), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011515723.1

ORF Insert Sequence:

ATGGCCAAGC GCAGCTCGCT GTACATCCGC ATCGTGGAGG GGAAGAACCT TCCCGCCAAG 
GACATCACTG GCAGCAGCGA CCCCTACTGC ATCGTGAAGG TGGACAATGA GCCCATCATC 
AGGACAGCCA CAGTGTGGAA GACCCTGTGC CCCTTCTGGG GTGAGGAGTA CCAAGTGCAC 
CTGCCGCCCA CCTTCCACGC TGTGGCTTTC TATGTCATGG ATGAGGATGC CCTCAGCCGG 
GACGACGTTA TCGGAAAGGT CTGCCTTACA AGGGACACCA TAGCCTCTCA CCCTAAGGGT 
TTCAGCGGGT GGGCCCACCT GACGGAGGTC GACCCCGATG AGGAGGTGCA GGGCGAGATC 
CACCTGCGGC TGGAAGTGTG GCCAGGGGCC CGGGCCTGCC GGCTACGCTG CTCTGTGCTG 
GAGGCCAGGG ATCTGGCCCC AAAGGACCGC AATGGCACAT CTGACCCCTT CGTCCGAGTG 
CGCTACAAGG GCCGGACACG GGAGACCTCG ATCGTGAAGA AGTCATGCTA CCCACGCTGG 
AATGAGACGT TTGAATTTGA GCTGCAGGAG GGGGCCATGG AGGCGCTGTG CGTGGAGGCC 
TGGGACTGGG ACCTTGTCAG CCGAAACGAC TTCCTGGGCA AAGTGGTGAT TGATGTCCAG 
AGACTGCGGG TGGTGCAGCA GGAGGAGGGC TGGTTCCGGC TGCAGCCCGA CCAGTCCAAG 
AGCCGGCGGC ATGACGAGGG CAACCTGGGC TCCTTGCAGC TGGAGGTGCG GCTGCGGGAC 
GAGACGGTGC TGCCCTCCAG CTACTACCAG CCACTGGTGC ACCTGCTGTG CCACGAGGTC 
AAGCTGGGCA TGCAGGGCCC AGGGCAGCTG ATCCCACTCA TCGAGGAGAC AACCAGCACC 
GAGTGTCGCC AGGACGTGGC CACGAACCTG CTCAAGCTCT TCCTGGGGCA GGGGCTGGCC 
AAGGACTTCC TGGACCTGCT CTTCCAGCTG GAGCTGAGTC GCACCAGTGA GACCAACACC 
CTGTTCCGGA GCAACTCTCT GGCCTCAAAG TCCATGGAGT CTTTTCTGAA GGTGGCCGGG 
ATGCAGTACC TGCACGGCGT CCTGGGCCCC ATCATCAACA AGGTGTTTGA GGAGAAGAAG 
TACGTGGAGC TGGACCCCAG CAAAGTGGAA GTTAAGGATG TAGGGTGCTC CGGGCTGCAC 
CGCCCGCAGA CCGAGGCCGA GGTGCTGGAG CAGAGCGCGC AGACGCTGCG CGCCCACCTG 
GGGGCCCTGC TGAGCGCGCT CAGCCGCTCG GTTCGCGCGT GCCCCGCCGT GGTGCGCGCC 
ACCTTCCGCC AGCTCTTCCG GCGCGTGCGC GAGCGCTTCC CCGGCGCCCA GCACGAGAAT 
GTACCGTTCA TCGCCGTCAC CAGCTTCCTG TGCCTGCGCT TCTTCTCTCC CGCCATCATG 
TCGCCCAAGC TCTTCCACCT GCGGGAGCGC CACGCGGACG CCCGCACCAG CCGCACCCTG 
CTCCTGTTGG CCAAGGCAGT CCAGAACGTG GGCAACATGG ACACGCCGGC TTCCAGGGCC 
AAGGAGGCTT GGATGGAGCC GCTGCAGCCC ACCGTGCGCC AGGGCGTGGC GCAGCTGAAG 
GACTTCATCA CCAAGCTCGT GGACATCGAG GAGAAGGACG AGCTGGACCT GCAGCGGACG 
CTGAGTTTGC AGGCGCCACC TGTGAAGGAG GGGCCACTCT TCATCCACAG GACCAAGGGC 
AAGGGCCCCC TCATGTCCTC CTCCTTCAAG AAGCTCTACT TCTCCCTCAC TACCGAGGCC 
CTCAGCTTCG CGAAGACGCC CAGCTCCAAG GAGTTCCCTT TTGTGTCAGG GTCCAGAGCC 
ACTGGGAAAA GTAGTGATAT CAGGGCCACC AAATATATTT GGCGGGTTCT GGAATACCTA 
CGAGCGTGGC CAAGGGGCCA GCGGCGGCTG AAATCCAGCC CACACCAGCC TCCTGGGCTC 
CTACCATCCC GGTGCCTTCC GTGGGGACAA GTGGAGCTGC TGCCACCAAA AAGACGAGAC 
AGTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASA4 ( NM_006989.6 ) cDNA ORF clone, Homo sapiens(human) -> NP_008920.5 Homo sapiens RAS p21 protein activator 4 (RASA4), transcript variant 1, mRNA.

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CloneID	OHu01164
Clone ID Related Accession (Same CDS sequence)	NM_006989.6 , NM_006989.5
Accession Version	NM_006989.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2412bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-21
Organism	Homo sapiens(human)
Product	ras GTPase-activating protein 4 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC105052.3 and AC093668.4. On Nov 22, 2019 this sequence version replaced NM_006989.5. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). ##Evidence-Data-START## Transcript exon combination :: AB011110.2, BC110873.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCCAAGC GCAGCTCGCT GTACATCCGC ATCGTGGAGG GGAAGAACCT TCCCGCCAAG 
GACATCACTG GCAGCAGCGA CCCCTACTGC ATCGTGAAGG TGGACAATGA GCCCATCATC 
AGGACAGCCA CAGTGTGGAA GACCCTGTGC CCCTTCTGGG GTGAGGAGTA CCAAGTGCAC 
CTGCCGCCCA CCTTCCACGC TGTGGCTTTC TATGTCATGG ATGAGGATGC CCTCAGCCGG 
GACGACGTTA TCGGAAAGGT CTGCCTTACA AGGGACACCA TAGCCTCTCA CCCTAAGGGT 
TTCAGCGGGT GGGCCCACCT GACGGAGGTC GACCCCGATG AGGAGGTGCA GGGCGAGATC 
CACCTGCGGC TGGAAGTGTG GCCAGGGGCC CGGGCCTGCC GGCTACGCTG CTCTGTGCTG 
GAGGCCAGGG ATCTGGCCCC AAAGGACCGC AATGGCACAT CTGACCCCTT CGTCCGAGTG 
CGCTACAAGG GCCGGACACG GGAGACCTCG ATCGTGAAGA AGTCATGCTA CCCACGCTGG 
AATGAGACGT TTGAATTTGA GCTGCAGGAG GGGGCCATGG AGGCGCTGTG CGTGGAGGCC 
TGGGACTGGG ACCTTGTCAG CCGAAACGAC TTCCTGGGCA AAGTGGTGAT TGATGTCCAG 
AGACTGCGGG TGGTGCAGCA GGAGGAGGGC TGGTTCCGGC TGCAGCCCGA CCAGTCCAAG 
AGCCGGCGGC ATGACGAGGG CAACCTGGGC TCCTTGCAGC TGGAGGTGCG GCTGCGGGAC 
GAGACGGTGC TGCCCTCCAG CTACTACCAG CCACTGGTGC ACCTGCTGTG CCACGAGGTC 
AAGCTGGGCA TGCAGGGCCC AGGGCAGCTG ATCCCACTCA TCGAGGAGAC AACCAGCACC 
GAGTGTCGCC AGGACGTGGC CACGAACCTG CTCAAGCTCT TCCTGGGGCA GGGGCTGGCC 
AAGGACTTCC TGGACCTGCT CTTCCAGCTG GAGCTGAGTC GCACCAGTGA GACCAACACC 
CTGTTCCGGA GCAACTCTCT GGCCTCAAAG TCCATGGAGT CTTTTCTGAA GGTGGCCGGG 
ATGCAGTACC TGCACGGCGT CCTGGGCCCC ATCATCAACA AGGTGTTTGA GGAGAAGAAG 
TACGTGGAGC TGGACCCCAG CAAAGTGGAA GTTAAGGATG TAGGGTGCTC CGGGCTGCAC 
CGCCCGCAGA CCGAGGCCGA GGTGCTGGAG CAGAGCGCGC AGACGCTGCG CGCCCACCTG 
GGGGCCCTGC TGAGCGCGCT CAGCCGCTCG GTTCGCGCGT GCCCCGCCGT GGTGCGCGCC 
ACCTTCCGCC AGCTCTTCCG GCGCGTGCGC GAGCGCTTCC CCGGCGCCCA GCACGAGAAT 
GTACCGTTCA TCGCCGTCAC CAGCTTCCTG TGCCTGCGCT TCTTCTCTCC CGCCATCATG 
TCGCCCAAGC TCTTCCACCT GCGGGAGCGC CACGCGGACG CCCGCACCAG CCGCACCCTG 
CTCCTGTTGG CCAAGGCAGT CCAGAACGTG GGCAACATGG ACACGCCGGC TTCCAGGGCC 
AAGGAGGCTT GGATGGAGCC GCTGCAGCCC ACCGTGCGCC AGGGCGTGGC GCAGCTGAAG 
GACTTCATCA CCAAGCTCGT GGACATCGAG GAGAAGGACG AGCTGGACCT GCAGCGGACG 
CTGAGTTTGC AGGCGCCACC TGTGAAGGAG GGGCCACTCT TCATCCACAG GACCAAGGGC 
AAGGGCCCCC TCATGTCCTC CTCCTTCAAG AAGCTCTACT TCTCCCTCAC TACCGAGGCC 
CTCAGCTTCG CGAAGACGCC CAGCTCCAAG AAAAGCGCCC TCATCAAGTT AGCCAACATC 
CGGGCAGCGG AAAAGGTTGA GGAAAAGAGC TTTGGCGGCT CGCACGTCAT GCAGGTCATC 
TACACGGACG ACGCCGGCAG GCCCCAGACT GCCTACCTGC AGTGCAAGTG TGTGAATGAG 
CTTAACCAGT GGCTGTCTGC GCTGCGGAAG GTGAGCATCA ACAACACCGG ACTGCTGGGC 
TCCTACCACC CTGGCGTCTT CCGTGGGGAC AAGTGGAGCT GCTGCCACCA AAAAGAGAAG 
ACAGGTCAGG GCTGCGATAA GACCCGGTCA CGGGTGACCC TGCAGGAGTG GAATGACCCT 
CTTGACCATG ACCTTGAGGC CCAGCTCATC TACCGGCACC TGCTGGGCGT GGAGGCCATG 
CTGTGGGAGA GGCACCGGGA GCTGAGCGGG GGCGCAGAGG CAGGCACGGT GCCCACGAGC 
CCTGGCAAAG TCCCCGAGGA CTCATTGGCC CGGCTGCTCC GGGTGCTGCA GGACCTCCGC 
GAGGCCCATA GCTCCAGCCC GGCCGGCTCC CCACCCTCAG AGCCCAACTG CCTCCTGGAG 
CTGCAGACGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_008920.5
CDS	68..2479
Translation	MAKRSSLYIRIVEGKNLPAKDITGSSDPYCIVKVDNEPIIRTATVWKTLCPFWGEEYQVHLPPTFHAVAFYVMDEDALSRDDVIGKVCLTRDTIASHPKGFSGWAHLTEVDPDEEVQGEIHLRLEVWPGARACRLRCSVLEARDLAPKDRNGTSDPFVRVRYKGRTRETSIVKKSCYPRWNETFEFELQEGAMEALCVEAWDWDLVSRNDFLGKVVIDVQRLRVVQQEEGWFRLQPDQSKSRRHDEGNLGSLQLEVRLRDETVLPSSYYQPLVHLLCHEVKLGMQGPGQLIPLIEETTSTECRQDVATNLLKLFLGQGLAKDFLDLLFQLELSRTSETNTLFRSNSLASKSMESFLKVAGMQYLHGVLGPIINKVFEEKKYVELDPSKVEVKDVGCSGLHRPQTEAEVLEQSAQTLRAHLGALLSALSRSVRACPAVVRATFRQLFRRVRERFPGAQHENVPFIAVTSFLCLRFFSPAIMSPKLFHLRERHADARTSRTLLLLAKAVQNVGNMDTPASRAKEAWMEPLQPTVRQGVAQLKDFITKLVDIEEKDELDLQRTLSLQAPPVKEGPLFIHRTKGKGPLMSSSFKKLYFSLTTEALSFAKTPSSKKSALIKLANIRAAEKVEEKSFGGSHVMQVIYTDDAGRPQTAYLQCKCVNELNQWLSALRKVSINNTGLLGSYHPGVFRGDKWSCCHQKEKTGQGCDKTRSRVTLQEWNDPLDHDLEAQLIYRHLLGVEAMLWERHRELSGGAEAGTVPTSPGKVPEDSLARLLRVLQDLREAHSSSPAGSPPSEPNCLLELQT

Target ORF information:

RefSeq Version	NM_006989.6
Organism	Homo sapiens(human)
Definition	Homo sapiens RAS p21 protein activator 4 (RASA4), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006989.6

ORF Insert Sequence:

ATGGCCAAGC GCAGCTCGCT GTACATCCGC ATCGTGGAGG GGAAGAACCT TCCCGCCAAG 
GACATCACTG GCAGCAGCGA CCCCTACTGC ATCGTGAAGG TGGACAATGA GCCCATCATC 
AGGACAGCCA CAGTGTGGAA GACCCTGTGC CCCTTCTGGG GTGAGGAGTA CCAAGTGCAC 
CTGCCGCCCA CCTTCCACGC TGTGGCTTTC TATGTCATGG ATGAGGATGC CCTCAGCCGG 
GACGACGTTA TCGGAAAGGT CTGCCTTACA AGGGACACCA TAGCCTCTCA CCCTAAGGGT 
TTCAGCGGGT GGGCCCACCT GACGGAGGTC GACCCCGATG AGGAGGTGCA GGGCGAGATC 
CACCTGCGGC TGGAAGTGTG GCCAGGGGCC CGGGCCTGCC GGCTACGCTG CTCTGTGCTG 
GAGGCCAGGG ATCTGGCCCC AAAGGACCGC AATGGCACAT CTGACCCCTT CGTCCGAGTG 
CGCTACAAGG GCCGGACACG GGAGACCTCG ATCGTGAAGA AGTCATGCTA CCCACGCTGG 
AATGAGACGT TTGAATTTGA GCTGCAGGAG GGGGCCATGG AGGCGCTGTG CGTGGAGGCC 
TGGGACTGGG ACCTTGTCAG CCGAAACGAC TTCCTGGGCA AAGTGGTGAT TGATGTCCAG 
AGACTGCGGG TGGTGCAGCA GGAGGAGGGC TGGTTCCGGC TGCAGCCCGA CCAGTCCAAG 
AGCCGGCGGC ATGACGAGGG CAACCTGGGC TCCTTGCAGC TGGAGGTGCG GCTGCGGGAC 
GAGACGGTGC TGCCCTCCAG CTACTACCAG CCACTGGTGC ACCTGCTGTG CCACGAGGTC 
AAGCTGGGCA TGCAGGGCCC AGGGCAGCTG ATCCCACTCA TCGAGGAGAC AACCAGCACC 
GAGTGTCGCC AGGACGTGGC CACGAACCTG CTCAAGCTCT TCCTGGGGCA GGGGCTGGCC 
AAGGACTTCC TGGACCTGCT CTTCCAGCTG GAGCTGAGTC GCACCAGTGA GACCAACACC 
CTGTTCCGGA GCAACTCTCT GGCCTCAAAG TCCATGGAGT CTTTTCTGAA GGTGGCCGGG 
ATGCAGTACC TGCACGGCGT CCTGGGCCCC ATCATCAACA AGGTGTTTGA GGAGAAGAAG 
TACGTGGAGC TGGACCCCAG CAAAGTGGAA GTTAAGGATG TAGGGTGCTC CGGGCTGCAC 
CGCCCGCAGA CCGAGGCCGA GGTGCTGGAG CAGAGCGCGC AGACGCTGCG CGCCCACCTG 
GGGGCCCTGC TGAGCGCGCT CAGCCGCTCG GTTCGCGCGT GCCCCGCCGT GGTGCGCGCC 
ACCTTCCGCC AGCTCTTCCG GCGCGTGCGC GAGCGCTTCC CCGGCGCCCA GCACGAGAAT 
GTACCGTTCA TCGCCGTCAC CAGCTTCCTG TGCCTGCGCT TCTTCTCTCC CGCCATCATG 
TCGCCCAAGC TCTTCCACCT GCGGGAGCGC CACGCGGACG CCCGCACCAG CCGCACCCTG 
CTCCTGTTGG CCAAGGCAGT CCAGAACGTG GGCAACATGG ACACGCCGGC TTCCAGGGCC 
AAGGAGGCTT GGATGGAGCC GCTGCAGCCC ACCGTGCGCC AGGGCGTGGC GCAGCTGAAG 
GACTTCATCA CCAAGCTCGT GGACATCGAG GAGAAGGACG AGCTGGACCT GCAGCGGACG 
CTGAGTTTGC AGGCGCCACC TGTGAAGGAG GGGCCACTCT TCATCCACAG GACCAAGGGC 
AAGGGCCCCC TCATGTCCTC CTCCTTCAAG AAGCTCTACT TCTCCCTCAC TACCGAGGCC 
CTCAGCTTCG CGAAGACGCC CAGCTCCAAG AAAAGCGCCC TCATCAAGTT AGCCAACATC 
CGGGCAGCGG AAAAGGTTGA GGAAAAGAGC TTTGGCGGCT CGCACGTCAT GCAGGTCATC 
TACACGGACG ACGCCGGCAG GCCCCAGACT GCCTACCTGC AGTGCAAGTG TGTGAATGAG 
CTTAACCAGT GGCTGTCTGC GCTGCGGAAG GTGAGCATCA ACAACACCGG ACTGCTGGGC 
TCCTACCACC CTGGCGTCTT CCGTGGGGAC AAGTGGAGCT GCTGCCACCA AAAAGAGAAG 
ACAGGTCAGG GCTGCGATAA GACCCGGTCA CGGGTGACCC TGCAGGAGTG GAATGACCCT 
CTTGACCATG ACCTTGAGGC CCAGCTCATC TACCGGCACC TGCTGGGCGT GGAGGCCATG 
CTGTGGGAGA GGCACCGGGA GCTGAGCGGG GGCGCAGAGG CAGGCACGGT GCCCACGAGC 
CCTGGCAAAG TCCCCGAGGA CTCATTGGCC CGGCTGCTCC GGGTGCTGCA GGACCTCCGC 
GAGGCCCATA GCTCCAGCCC GGCCGGCTCC CCACCCTCAG AGCCCAACTG CCTCCTGGAG 
CTGCAGACGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASA4 ( XM_017011661.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016867150.1 Homo sapiens RAS p21 protein activator 4 (RASA4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu96998
Clone ID Related Accession (Same CDS sequence)	XM_017011661.1 , XM_017011661.2
Accession Version	XM_017011661.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2523bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	ras GTPase-activating protein 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGC GCAGCTCGCT GTACATCCGC ATCGTGGAGG GGAAGAACCT TCCCGCCAAG 
GACATCACTG GCAGCAGCGA CCCCTACTGC ATCGTGAAGG TGGACAATGA GCCCATCATC 
AGGACAGCCA CAGTGTGGAA GACCCTGTGC CCCTTCTGGG GTGAGGAGTA CCAAGTGCAC 
CTGCCGCCCA CCTTCCACGC TGTGGCTTTC TATGTCATGG ATGAGGATGC CCTCAGCCGG 
GACGACGTTA TCGGAAAGGT CTGCCTTACA AGGGACACCA TAGCCTCTCA CCCTAAGGGT 
TTCAGCGGGT GGGCCCACCT GACGGAGGTC GACCCCGATG AGGAGGTGCA GGGCGAGATC 
CACCTGCGGC TGGAAGTGTG GCCAGGGGCC CGGGCCTGCC GGCTACGCTG CTCTGTGCTG 
GAGGCCAGGG ATCTGGCCCC AAAGGACCGC AATGGCACAT CTGACCCCTT CGTCCGAGTG 
CGCTACAAGG GCCGGACACG GGAGACCTCG ATCGTGAAGA AGTCATGCTA CCCACGCTGG 
AATGAGACGT TTGAATTTGA GCTGCAGGAG GGGGCCATGG AGGCGCTGTG CGTGGAGGCC 
TGGGACTGGG ACCTTGTCAG CCGAAACGAC TTCCTGGGCA AAGTGGTGAT TGATGTCCAG 
AGACTGCGGG TGGTGCAGCA GGAGGAGGGC TGGTTCCGGC TGCAGCCCGA CCAGTCCAAG 
AGCCGGCGGC ATGACGAGGG CAACCTGGGC TCCTTGCAGC TGGAGGTGCG GCTGCGGGAC 
GAGACGGTGC TGCCCTCCAG CTACTACCAG CCACTGGTGC ACCTGCTGTG CCACGAGGTC 
AAGCTGGGCA TGCAGGGCCC AGGGCAGCTG ATCCCACTCA TCGAGGAGAC AACCAGCACC 
GAGTGTCGCC AGGACGTGGC CACGAACCTG CTCAAGCTCT TCCTGGGGCA GGGGCTGGCC 
AAGGACTTCC TGGACCTGCT CTTCCAGCTG GAGCTGAGTC GCACCAGTGA GACCAACACC 
CTGTTCCGGA GCAACTCTCT GGCCTCAAAG TCCATGGAGT CTTTTCTGAA GGTGGCCGGG 
ATGCAGTACC TGCACGGCGT CCTGGGCCCC ATCATCAACA AGGTGTTTGA GGAGAAGAAG 
TACGTGGAGC TGGACCCCAG CAAAGTGGAA GTTAAGGATG TAGGGTGCTC CGGGCTGCAC 
CGCCCGCAGA CCGAGGCCGA GGTGCTGGAG CAGAGCGCGC AGACGCTGCG CGCCCACCTG 
GGGGCCCTGC TGAGCGCGCT CAGCCGCTCG GTTCGCGCGT GCCCCGCCGT GGTGCGCGCC 
ACCTTCCGCC AGCTCTTCCG GCGCGTGCGC GAGCGCTTCC CCGGCGCCCA GCACGAGAAT 
GTACCGTTCA TCGCCGTCAC CAGCTTCCTG TGCCTGCGCT TCTTCTCTCC CGCCATCATG 
TCGCCCAAGC TCTTCCACCT GCGGGAGCGC CACGCGGACG CCCGCACCAG CCGCACCCTG 
CTCCTGTTGG CCAAGGCAGT CCAGAACGTG GGCAACATGG ACACGCCGGC TTCCAGGGCC 
AAGGAGGCTT GGATGGAGCC GCTGCAGCCC ACCGTGCGCC AGGGCGTGGC GCAGCTGAAG 
GACTTCATCA CCAAGCTCGT GGACATCGAG GAGAAGGACG AGCTGGACCT GCAGCGGACG 
CTGAGTTTGC AGGCGCCACC TGTGAAGGAG GGGCCACTCT TCATCCACAG GACCAAGGGC 
AAGGGCCCCC TCATGTCCTC CTCCTTCAAG AAGCTCTACT TCTCCCTCAC TACCGAGGCC 
CTCAGCTTCG CGAAGACGCC CAGCTCCAAG AAAAGCGCCC TCATCAAGTT AGCCAACATC 
CGGGCAGCGG AAAAGGTTGA GGAAAAGAGC TTTGGCGGCT CGCACGTCAT GCAGGTCATC 
TACACGGACG ACGCCGGCAG GCCCCAGACT GCCTACCTGC AGTGCAAGTG TGTGAATGAG 
CTTAACCAGT GGCTGTCTGC GCTGCGGAAG GTGAGCATCA ACAACACCGG ACTGCTGGGC 
TCCTACCACC CTGGCGTCTT CCGTGGGGAC AAGTGGAGCT GCTGCCACCA AAAAGAGAAG 
ACAGGTCAGG GCTGCGATAA GACCCGGTCA CGGGTGACCC TGCAGGAGTG GAATGACCCT 
CTTGACCATG ACCTTGAGGC CCAGCTCATC TACCGGCACC TGCTGGGCGT GGAGGCCATG 
CTGTGGGAGA GGCACCGGGA GCTGAGCGGG GGCGCAGAGG CAGGCACGGT GCCCACGAGC 
CCTGGCAAAG GTAGGTCTTG CCCACCTGGG ACTTGCTCGT GGCCCGAGCC CCCTCCAGAG 
ATGCACGGGT GGGGTCCTCG GGCTCACTCC CAGGGGCCCT CACGGCTGCC TCTGCCCACA 
GTCCCCGAGG ACTCATTGGC CCGGCTGCTC CGGGTGCTGC AGGACCTCCG CGAGGCCCAT 
AGCTCCAGCC CGGCCGGCTC CCCACCCTCA GAGCCCAACT GCCTCCTGGA GCTGCAGACG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016867150.1
CDS	69..2591
Translation	MAKRSSLYIRIVEGKNLPAKDITGSSDPYCIVKVDNEPIIRTATVWKTLCPFWGEEYQVHLPPTFHAVAFYVMDEDALSRDDVIGKVCLTRDTIASHPKGFSGWAHLTEVDPDEEVQGEIHLRLEVWPGARACRLRCSVLEARDLAPKDRNGTSDPFVRVRYKGRTRETSIVKKSCYPRWNETFEFELQEGAMEALCVEAWDWDLVSRNDFLGKVVIDVQRLRVVQQEEGWFRLQPDQSKSRRHDEGNLGSLQLEVRLRDETVLPSSYYQPLVHLLCHEVKLGMQGPGQLIPLIEETTSTECRQDVATNLLKLFLGQGLAKDFLDLLFQLELSRTSETNTLFRSNSLASKSMESFLKVAGMQYLHGVLGPIINKVFEEKKYVELDPSKVEVKDVGCSGLHRPQTEAEVLEQSAQTLRAHLGALLSALSRSVRACPAVVRATFRQLFRRVRERFPGAQHENVPFIAVTSFLCLRFFSPAIMSPKLFHLRERHADARTSRTLLLLAKAVQNVGNMDTPASRAKEAWMEPLQPTVRQGVAQLKDFITKLVDIEEKDELDLQRTLSLQAPPVKEGPLFIHRTKGKGPLMSSSFKKLYFSLTTEALSFAKTPSSKKSALIKLANIRAAEKVEEKSFGGSHVMQVIYTDDAGRPQTAYLQCKCVNELNQWLSALRKVSINNTGLLGSYHPGVFRGDKWSCCHQKEKTGQGCDKTRSRVTLQEWNDPLDHDLEAQLIYRHLLGVEAMLWERHRELSGGAEAGTVPTSPGKGRSCPPGTCSWPEPPPEMHGWGPRAHSQGPSRLPLPTVPEDSLARLLRVLQDLREAHSSSPAGSPPSEPNCLLELQT

Target ORF information:

RefSeq Version	XM_017011661.1
Organism	Homo sapiens(human)
Definition	Homo sapiens RAS p21 protein activator 4 (RASA4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017011661.1

ORF Insert Sequence:

ATGGCCAAGC GCAGCTCGCT GTACATCCGC ATCGTGGAGG GGAAGAACCT TCCCGCCAAG 
GACATCACTG GCAGCAGCGA CCCCTACTGC ATCGTGAAGG TGGACAATGA GCCCATCATC 
AGGACAGCCA CAGTGTGGAA GACCCTGTGC CCCTTCTGGG GTGAGGAGTA CCAAGTGCAC 
CTGCCGCCCA CCTTCCACGC TGTGGCTTTC TATGTCATGG ATGAGGATGC CCTCAGCCGG 
GACGACGTTA TCGGAAAGGT CTGCCTTACA AGGGACACCA TAGCCTCTCA CCCTAAGGGT 
TTCAGCGGGT GGGCCCACCT GACGGAGGTC GACCCCGATG AGGAGGTGCA GGGCGAGATC 
CACCTGCGGC TGGAAGTGTG GCCAGGGGCC CGGGCCTGCC GGCTACGCTG CTCTGTGCTG 
GAGGCCAGGG ATCTGGCCCC AAAGGACCGC AATGGCACAT CTGACCCCTT CGTCCGAGTG 
CGCTACAAGG GCCGGACACG GGAGACCTCG ATCGTGAAGA AGTCATGCTA CCCACGCTGG 
AATGAGACGT TTGAATTTGA GCTGCAGGAG GGGGCCATGG AGGCGCTGTG CGTGGAGGCC 
TGGGACTGGG ACCTTGTCAG CCGAAACGAC TTCCTGGGCA AAGTGGTGAT TGATGTCCAG 
AGACTGCGGG TGGTGCAGCA GGAGGAGGGC TGGTTCCGGC TGCAGCCCGA CCAGTCCAAG 
AGCCGGCGGC ATGACGAGGG CAACCTGGGC TCCTTGCAGC TGGAGGTGCG GCTGCGGGAC 
GAGACGGTGC TGCCCTCCAG CTACTACCAG CCACTGGTGC ACCTGCTGTG CCACGAGGTC 
AAGCTGGGCA TGCAGGGCCC AGGGCAGCTG ATCCCACTCA TCGAGGAGAC AACCAGCACC 
GAGTGTCGCC AGGACGTGGC CACGAACCTG CTCAAGCTCT TCCTGGGGCA GGGGCTGGCC 
AAGGACTTCC TGGACCTGCT CTTCCAGCTG GAGCTGAGTC GCACCAGTGA GACCAACACC 
CTGTTCCGGA GCAACTCTCT GGCCTCAAAG TCCATGGAGT CTTTTCTGAA GGTGGCCGGG 
ATGCAGTACC TGCACGGCGT CCTGGGCCCC ATCATCAACA AGGTGTTTGA GGAGAAGAAG 
TACGTGGAGC TGGACCCCAG CAAAGTGGAA GTTAAGGATG TAGGGTGCTC CGGGCTGCAC 
CGCCCGCAGA CCGAGGCCGA GGTGCTGGAG CAGAGCGCGC AGACGCTGCG CGCCCACCTG 
GGGGCCCTGC TGAGCGCGCT CAGCCGCTCG GTTCGCGCGT GCCCCGCCGT GGTGCGCGCC 
ACCTTCCGCC AGCTCTTCCG GCGCGTGCGC GAGCGCTTCC CCGGCGCCCA GCACGAGAAT 
GTACCGTTCA TCGCCGTCAC CAGCTTCCTG TGCCTGCGCT TCTTCTCTCC CGCCATCATG 
TCGCCCAAGC TCTTCCACCT GCGGGAGCGC CACGCGGACG CCCGCACCAG CCGCACCCTG 
CTCCTGTTGG CCAAGGCAGT CCAGAACGTG GGCAACATGG ACACGCCGGC TTCCAGGGCC 
AAGGAGGCTT GGATGGAGCC GCTGCAGCCC ACCGTGCGCC AGGGCGTGGC GCAGCTGAAG 
GACTTCATCA CCAAGCTCGT GGACATCGAG GAGAAGGACG AGCTGGACCT GCAGCGGACG 
CTGAGTTTGC AGGCGCCACC TGTGAAGGAG GGGCCACTCT TCATCCACAG GACCAAGGGC 
AAGGGCCCCC TCATGTCCTC CTCCTTCAAG AAGCTCTACT TCTCCCTCAC TACCGAGGCC 
CTCAGCTTCG CGAAGACGCC CAGCTCCAAG AAAAGCGCCC TCATCAAGTT AGCCAACATC 
CGGGCAGCGG AAAAGGTTGA GGAAAAGAGC TTTGGCGGCT CGCACGTCAT GCAGGTCATC 
TACACGGACG ACGCCGGCAG GCCCCAGACT GCCTACCTGC AGTGCAAGTG TGTGAATGAG 
CTTAACCAGT GGCTGTCTGC GCTGCGGAAG GTGAGCATCA ACAACACCGG ACTGCTGGGC 
TCCTACCACC CTGGCGTCTT CCGTGGGGAC AAGTGGAGCT GCTGCCACCA AAAAGAGAAG 
ACAGGTCAGG GCTGCGATAA GACCCGGTCA CGGGTGACCC TGCAGGAGTG GAATGACCCT 
CTTGACCATG ACCTTGAGGC CCAGCTCATC TACCGGCACC TGCTGGGCGT GGAGGCCATG 
CTGTGGGAGA GGCACCGGGA GCTGAGCGGG GGCGCAGAGG CAGGCACGGT GCCCACGAGC 
CCTGGCAAAG GTAGGTCTTG CCCACCTGGG ACTTGCTCGT GGCCCGAGCC CCCTCCAGAG 
ATGCACGGGT GGGGTCCTCG GGCTCACTCC CAGGGGCCCT CACGGCTGCC TCTGCCCACA 
GTCCCCGAGG ACTCATTGGC CCGGCTGCTC CGGGTGCTGC AGGACCTCCG CGAGGCCCAT 
AGCTCCAGCC CGGCCGGCTC CCCACCCTCA GAGCCCAACT GCCTCCTGGA GCTGCAGACG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASA4 ( XM_017011661.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016867150.1 Homo sapiens RAS p21 protein activator 4 (RASA4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu96998
Clone ID Related Accession (Same CDS sequence)	XM_017011661.1 , XM_017011661.2
Accession Version	XM_017011661.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2523bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ras GTPase-activating protein 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017011661.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGC GCAGCTCGCT GTACATCCGC ATCGTGGAGG GGAAGAACCT TCCCGCCAAG 
GACATCACTG GCAGCAGCGA CCCCTACTGC ATCGTGAAGG TGGACAATGA GCCCATCATC 
AGGACAGCCA CAGTGTGGAA GACCCTGTGC CCCTTCTGGG GTGAGGAGTA CCAAGTGCAC 
CTGCCGCCCA CCTTCCACGC TGTGGCTTTC TATGTCATGG ATGAGGATGC CCTCAGCCGG 
GACGACGTTA TCGGAAAGGT CTGCCTTACA AGGGACACCA TAGCCTCTCA CCCTAAGGGT 
TTCAGCGGGT GGGCCCACCT GACGGAGGTC GACCCCGATG AGGAGGTGCA GGGCGAGATC 
CACCTGCGGC TGGAAGTGTG GCCAGGGGCC CGGGCCTGCC GGCTACGCTG CTCTGTGCTG 
GAGGCCAGGG ATCTGGCCCC AAAGGACCGC AATGGCACAT CTGACCCCTT CGTCCGAGTG 
CGCTACAAGG GCCGGACACG GGAGACCTCG ATCGTGAAGA AGTCATGCTA CCCACGCTGG 
AATGAGACGT TTGAATTTGA GCTGCAGGAG GGGGCCATGG AGGCGCTGTG CGTGGAGGCC 
TGGGACTGGG ACCTTGTCAG CCGAAACGAC TTCCTGGGCA AAGTGGTGAT TGATGTCCAG 
AGACTGCGGG TGGTGCAGCA GGAGGAGGGC TGGTTCCGGC TGCAGCCCGA CCAGTCCAAG 
AGCCGGCGGC ATGACGAGGG CAACCTGGGC TCCTTGCAGC TGGAGGTGCG GCTGCGGGAC 
GAGACGGTGC TGCCCTCCAG CTACTACCAG CCACTGGTGC ACCTGCTGTG CCACGAGGTC 
AAGCTGGGCA TGCAGGGCCC AGGGCAGCTG ATCCCACTCA TCGAGGAGAC AACCAGCACC 
GAGTGTCGCC AGGACGTGGC CACGAACCTG CTCAAGCTCT TCCTGGGGCA GGGGCTGGCC 
AAGGACTTCC TGGACCTGCT CTTCCAGCTG GAGCTGAGTC GCACCAGTGA GACCAACACC 
CTGTTCCGGA GCAACTCTCT GGCCTCAAAG TCCATGGAGT CTTTTCTGAA GGTGGCCGGG 
ATGCAGTACC TGCACGGCGT CCTGGGCCCC ATCATCAACA AGGTGTTTGA GGAGAAGAAG 
TACGTGGAGC TGGACCCCAG CAAAGTGGAA GTTAAGGATG TAGGGTGCTC CGGGCTGCAC 
CGCCCGCAGA CCGAGGCCGA GGTGCTGGAG CAGAGCGCGC AGACGCTGCG CGCCCACCTG 
GGGGCCCTGC TGAGCGCGCT CAGCCGCTCG GTTCGCGCGT GCCCCGCCGT GGTGCGCGCC 
ACCTTCCGCC AGCTCTTCCG GCGCGTGCGC GAGCGCTTCC CCGGCGCCCA GCACGAGAAT 
GTACCGTTCA TCGCCGTCAC CAGCTTCCTG TGCCTGCGCT TCTTCTCTCC CGCCATCATG 
TCGCCCAAGC TCTTCCACCT GCGGGAGCGC CACGCGGACG CCCGCACCAG CCGCACCCTG 
CTCCTGTTGG CCAAGGCAGT CCAGAACGTG GGCAACATGG ACACGCCGGC TTCCAGGGCC 
AAGGAGGCTT GGATGGAGCC GCTGCAGCCC ACCGTGCGCC AGGGCGTGGC GCAGCTGAAG 
GACTTCATCA CCAAGCTCGT GGACATCGAG GAGAAGGACG AGCTGGACCT GCAGCGGACG 
CTGAGTTTGC AGGCGCCACC TGTGAAGGAG GGGCCACTCT TCATCCACAG GACCAAGGGC 
AAGGGCCCCC TCATGTCCTC CTCCTTCAAG AAGCTCTACT TCTCCCTCAC TACCGAGGCC 
CTCAGCTTCG CGAAGACGCC CAGCTCCAAG AAAAGCGCCC TCATCAAGTT AGCCAACATC 
CGGGCAGCGG AAAAGGTTGA GGAAAAGAGC TTTGGCGGCT CGCACGTCAT GCAGGTCATC 
TACACGGACG ACGCCGGCAG GCCCCAGACT GCCTACCTGC AGTGCAAGTG TGTGAATGAG 
CTTAACCAGT GGCTGTCTGC GCTGCGGAAG GTGAGCATCA ACAACACCGG ACTGCTGGGC 
TCCTACCACC CTGGCGTCTT CCGTGGGGAC AAGTGGAGCT GCTGCCACCA AAAAGAGAAG 
ACAGGTCAGG GCTGCGATAA GACCCGGTCA CGGGTGACCC TGCAGGAGTG GAATGACCCT 
CTTGACCATG ACCTTGAGGC CCAGCTCATC TACCGGCACC TGCTGGGCGT GGAGGCCATG 
CTGTGGGAGA GGCACCGGGA GCTGAGCGGG GGCGCAGAGG CAGGCACGGT GCCCACGAGC 
CCTGGCAAAG GTAGGTCTTG CCCACCTGGG ACTTGCTCGT GGCCCGAGCC CCCTCCAGAG 
ATGCACGGGT GGGGTCCTCG GGCTCACTCC CAGGGGCCCT CACGGCTGCC TCTGCCCACA 
GTCCCCGAGG ACTCATTGGC CCGGCTGCTC CGGGTGCTGC AGGACCTCCG CGAGGCCCAT 
AGCTCCAGCC CGGCCGGCTC CCCACCCTCA GAGCCCAACT GCCTCCTGGA GCTGCAGACG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016867150.1
CDS	69..2591
Translation	MAKRSSLYIRIVEGKNLPAKDITGSSDPYCIVKVDNEPIIRTATVWKTLCPFWGEEYQVHLPPTFHAVAFYVMDEDALSRDDVIGKVCLTRDTIASHPKGFSGWAHLTEVDPDEEVQGEIHLRLEVWPGARACRLRCSVLEARDLAPKDRNGTSDPFVRVRYKGRTRETSIVKKSCYPRWNETFEFELQEGAMEALCVEAWDWDLVSRNDFLGKVVIDVQRLRVVQQEEGWFRLQPDQSKSRRHDEGNLGSLQLEVRLRDETVLPSSYYQPLVHLLCHEVKLGMQGPGQLIPLIEETTSTECRQDVATNLLKLFLGQGLAKDFLDLLFQLELSRTSETNTLFRSNSLASKSMESFLKVAGMQYLHGVLGPIINKVFEEKKYVELDPSKVEVKDVGCSGLHRPQTEAEVLEQSAQTLRAHLGALLSALSRSVRACPAVVRATFRQLFRRVRERFPGAQHENVPFIAVTSFLCLRFFSPAIMSPKLFHLRERHADARTSRTLLLLAKAVQNVGNMDTPASRAKEAWMEPLQPTVRQGVAQLKDFITKLVDIEEKDELDLQRTLSLQAPPVKEGPLFIHRTKGKGPLMSSSFKKLYFSLTTEALSFAKTPSSKKSALIKLANIRAAEKVEEKSFGGSHVMQVIYTDDAGRPQTAYLQCKCVNELNQWLSALRKVSINNTGLLGSYHPGVFRGDKWSCCHQKEKTGQGCDKTRSRVTLQEWNDPLDHDLEAQLIYRHLLGVEAMLWERHRELSGGAEAGTVPTSPGKGRSCPPGTCSWPEPPPEMHGWGPRAHSQGPSRLPLPTVPEDSLARLLRVLQDLREAHSSSPAGSPPSEPNCLLELQT

Target ORF information:

RefSeq Version	XM_017011661.2
Organism	Homo sapiens(human)
Definition	Homo sapiens RAS p21 protein activator 4 (RASA4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017011661.2

ORF Insert Sequence:

ATGGCCAAGC GCAGCTCGCT GTACATCCGC ATCGTGGAGG GGAAGAACCT TCCCGCCAAG 
GACATCACTG GCAGCAGCGA CCCCTACTGC ATCGTGAAGG TGGACAATGA GCCCATCATC 
AGGACAGCCA CAGTGTGGAA GACCCTGTGC CCCTTCTGGG GTGAGGAGTA CCAAGTGCAC 
CTGCCGCCCA CCTTCCACGC TGTGGCTTTC TATGTCATGG ATGAGGATGC CCTCAGCCGG 
GACGACGTTA TCGGAAAGGT CTGCCTTACA AGGGACACCA TAGCCTCTCA CCCTAAGGGT 
TTCAGCGGGT GGGCCCACCT GACGGAGGTC GACCCCGATG AGGAGGTGCA GGGCGAGATC 
CACCTGCGGC TGGAAGTGTG GCCAGGGGCC CGGGCCTGCC GGCTACGCTG CTCTGTGCTG 
GAGGCCAGGG ATCTGGCCCC AAAGGACCGC AATGGCACAT CTGACCCCTT CGTCCGAGTG 
CGCTACAAGG GCCGGACACG GGAGACCTCG ATCGTGAAGA AGTCATGCTA CCCACGCTGG 
AATGAGACGT TTGAATTTGA GCTGCAGGAG GGGGCCATGG AGGCGCTGTG CGTGGAGGCC 
TGGGACTGGG ACCTTGTCAG CCGAAACGAC TTCCTGGGCA AAGTGGTGAT TGATGTCCAG 
AGACTGCGGG TGGTGCAGCA GGAGGAGGGC TGGTTCCGGC TGCAGCCCGA CCAGTCCAAG 
AGCCGGCGGC ATGACGAGGG CAACCTGGGC TCCTTGCAGC TGGAGGTGCG GCTGCGGGAC 
GAGACGGTGC TGCCCTCCAG CTACTACCAG CCACTGGTGC ACCTGCTGTG CCACGAGGTC 
AAGCTGGGCA TGCAGGGCCC AGGGCAGCTG ATCCCACTCA TCGAGGAGAC AACCAGCACC 
GAGTGTCGCC AGGACGTGGC CACGAACCTG CTCAAGCTCT TCCTGGGGCA GGGGCTGGCC 
AAGGACTTCC TGGACCTGCT CTTCCAGCTG GAGCTGAGTC GCACCAGTGA GACCAACACC 
CTGTTCCGGA GCAACTCTCT GGCCTCAAAG TCCATGGAGT CTTTTCTGAA GGTGGCCGGG 
ATGCAGTACC TGCACGGCGT CCTGGGCCCC ATCATCAACA AGGTGTTTGA GGAGAAGAAG 
TACGTGGAGC TGGACCCCAG CAAAGTGGAA GTTAAGGATG TAGGGTGCTC CGGGCTGCAC 
CGCCCGCAGA CCGAGGCCGA GGTGCTGGAG CAGAGCGCGC AGACGCTGCG CGCCCACCTG 
GGGGCCCTGC TGAGCGCGCT CAGCCGCTCG GTTCGCGCGT GCCCCGCCGT GGTGCGCGCC 
ACCTTCCGCC AGCTCTTCCG GCGCGTGCGC GAGCGCTTCC CCGGCGCCCA GCACGAGAAT 
GTACCGTTCA TCGCCGTCAC CAGCTTCCTG TGCCTGCGCT TCTTCTCTCC CGCCATCATG 
TCGCCCAAGC TCTTCCACCT GCGGGAGCGC CACGCGGACG CCCGCACCAG CCGCACCCTG 
CTCCTGTTGG CCAAGGCAGT CCAGAACGTG GGCAACATGG ACACGCCGGC TTCCAGGGCC 
AAGGAGGCTT GGATGGAGCC GCTGCAGCCC ACCGTGCGCC AGGGCGTGGC GCAGCTGAAG 
GACTTCATCA CCAAGCTCGT GGACATCGAG GAGAAGGACG AGCTGGACCT GCAGCGGACG 
CTGAGTTTGC AGGCGCCACC TGTGAAGGAG GGGCCACTCT TCATCCACAG GACCAAGGGC 
AAGGGCCCCC TCATGTCCTC CTCCTTCAAG AAGCTCTACT TCTCCCTCAC TACCGAGGCC 
CTCAGCTTCG CGAAGACGCC CAGCTCCAAG AAAAGCGCCC TCATCAAGTT AGCCAACATC 
CGGGCAGCGG AAAAGGTTGA GGAAAAGAGC TTTGGCGGCT CGCACGTCAT GCAGGTCATC 
TACACGGACG ACGCCGGCAG GCCCCAGACT GCCTACCTGC AGTGCAAGTG TGTGAATGAG 
CTTAACCAGT GGCTGTCTGC GCTGCGGAAG GTGAGCATCA ACAACACCGG ACTGCTGGGC 
TCCTACCACC CTGGCGTCTT CCGTGGGGAC AAGTGGAGCT GCTGCCACCA AAAAGAGAAG 
ACAGGTCAGG GCTGCGATAA GACCCGGTCA CGGGTGACCC TGCAGGAGTG GAATGACCCT 
CTTGACCATG ACCTTGAGGC CCAGCTCATC TACCGGCACC TGCTGGGCGT GGAGGCCATG 
CTGTGGGAGA GGCACCGGGA GCTGAGCGGG GGCGCAGAGG CAGGCACGGT GCCCACGAGC 
CCTGGCAAAG GTAGGTCTTG CCCACCTGGG ACTTGCTCGT GGCCCGAGCC CCCTCCAGAG 
ATGCACGGGT GGGGTCCTCG GGCTCACTCC CAGGGGCCCT CACGGCTGCC TCTGCCCACA 
GTCCCCGAGG ACTCATTGGC CCGGCTGCTC CGGGTGCTGC AGGACCTCCG CGAGGCCCAT 
AGCTCCAGCC CGGCCGGCTC CCCACCCTCA GAGCCCAACT GCCTCCTGGA GCTGCAGACG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASA4 ( XM_017011662.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016867151.1 Homo sapiens RAS p21 protein activator 4 (RASA4), transcript variant X2, mRNA.

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CloneID	OHu96999
Clone ID Related Accession (Same CDS sequence)	XM_017011662.1 , XM_017011662.2
Accession Version	XM_017011662.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2385bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	ras GTPase-activating protein 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGC GCAGCTCGCT GTACATCCGC ATCGTGGAGG GGAAGAACCT TCCCGCCAAG 
GACATCACTG GCAGCAGCGA CCCCTACTGC ATCGTGAAGG TGGACAATGA GCCCATCATC 
AGGACAGCCA CAGTGTGGAA GACCCTGTGC CCCTTCTGGG GTGAGGAGTA CCAAGTGCAC 
CTGCCGCCCA CCTTCCACGC TGTGGCTTTC TATGTCATGG ATGAGGATGC CCTCAGCCGG 
GACGACGTTA TCGGAAAGGT CTGCCTTACA AGGGACACCA TAGCCTCTCA CCCTAAGGGT 
TTCAGCGGGT GGGCCCACCT GACGGAGGTC GACCCCGATG AGGAGGTGCA GGGCGAGATC 
CACCTGCGGC TGGAAGTGTG GCCAGGGGCC CGGGCCTGCC GGCTACGCTG CTCTGTGCTG 
GAGGCCAGGG ATCTGGCCCC AAAGGACCGC AATGGCACAT CTGACCCCTT CGTCCGAGTG 
CGCTACAAGG GCCGGACACG GGAGACCTCG ATCGTGAAGA AGTCATGCTA CCCACGCTGG 
AATGAGACGT TTGAATTTGA GCTGCAGGAG GGGGCCATGG AGGCGCTGTG CGTGGAGGCC 
TGGGACTGGG ACCTTGTCAG CCGAAACGAC TTCCTGGGCA AAGTGGTGAT TGATGTCCAG 
AGACTGCGGG TGGTGCAGCA GGAGGAGGGC TGGTTCCGGC TGCAGCCCGA CCAGTCCAAG 
AGCCGGCGGC ATGACGAGGG CAACCTGGGC TCCTTGCAGC TGGAGGTGCG GCTGCGGGAC 
GAGACGGTGC TGCCCTCCAG CTACTACCAG CCACTGGTGC ACCTGCTGTG CCACGAGGTC 
AAGCTGGGCA TGCAGGGCCC AGGGCAGCTG ATCCCACTCA TCGAGGAGAC AACCAGCACC 
GAGTGTCGCC AGGACGTGGC CACGAACCTG CTCAAGCTCT TCCTGGGGCA GGGGCTGGCC 
AAGGACTTCC TGGACCTGCT CTTCCAGCTG GAGCTGAGTC GCACCAGTGA GACCAACACC 
CTGTTCCGGA GCAACTCTCT GGCCTCAAAG TCCATGGAGT CTTTTCTGAA GGTGGCCGGG 
ATGCAGTACC TGCACGGCGT CCTGGGCCCC ATCATCAACA AGGTGTTTGA GGAGAAGAAG 
TACGTGGAGC TGGACCCCAG CAAAGTGGAA GTTAAGGATG TAGGGTGCTC CGGGCTGCAC 
CGCCCGCAGA CCGAGGCCGA GGTGCTGGAG CAGAGCGCGC AGACGCTGCG CGCCCACCTG 
GGGGCCCTGC TGAGCGCGCT CAGCCGCTCG GTTCGCGCGT GCCCCGCCGT GGTGCGCGCC 
ACCTTCCGCC AGCTCTTCCG GCGCGTGCGC GAGCGCTTCC CCGGCGCCCA GCACGAGAAT 
GTACCGTTCA TCGCCGTCAC CAGCTTCCTG TGCCTGCGCT TCTTCTCTCC CGCCATCATG 
TCGCCCAAGC TCTTCCACCT GCGGGAGCGC CACGCGGACG CCCGCACCAG CCGCACCCTG 
CTCCTGTTGG CCAAGGCAGT CCAGAACGTG GGCAACATGG ACACGCCGGC TTCCAGGGCC 
AAGGAGGCTT GGATGGAGCC GCTGCAGCCC ACCGTGCGCC AGGGCGTGGC GCAGCTGAAG 
GACTTCATCA CCAAGCTCGT GGACATCGAG GAGAAGGACG AGCTGGACCT GCAGCGGACG 
CTGAGTTTGC AGGCGCCACC TGTGAAGGAG GGGCCACTCT TCATCCACAG GACCAAGGGC 
AAGGGCCCCC TCATGTCCTC CTCCTTCAAG AAGCTCTACT TCTCCCTCAC TACCGAGGCC 
CTCAGCTTCG CGAAGACGCC CAGCTCCAAG TGTGTGAATG AGCTTAACCA GTGGCTGTCT 
GCGCTGCGGA AGGTGAGCAT CAACAACACC GGACTGCTGG GCTCCTACCA CCCTGGCGTC 
TTCCGTGGGG ACAAGTGGAG CTGCTGCCAC CAAAAAGAGA AGACAGGTCA GGGCTGCGAT 
AAGACCCGGT CACGGGTGAC CCTGCAGGAG TGGAATGACC CTCTTGACCA TGACCTTGAG 
GCCCAGCTCA TCTACCGGCA CCTGCTGGGC GTGGAGGCCA TGCTGTGGGA GAGGCACCGG 
GAGCTGAGCG GGGGCGCAGA GGCAGGCACG GTGCCCACGA GCCCTGGCAA AGGTAGGTCT 
TGCCCACCTG GGACTTGCTC GTGGCCCGAG CCCCCTCCAG AGATGCACGG GTGGGGTCCT 
CGGGCTCACT CCCAGGGGCC CTCACGGCTG CCTCTGCCCA CAGTCCCCGA GGACTCATTG 
GCCCGGCTGC TCCGGGTGCT GCAGGACCTC CGCGAGGCCC ATAGCTCCAG CCCGGCCGGC 
TCCCCACCCT CAGAGCCCAA CTGCCTCCTG GAGCTGCAGA CGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016867151.1
CDS	69..2453
Translation	MAKRSSLYIRIVEGKNLPAKDITGSSDPYCIVKVDNEPIIRTATVWKTLCPFWGEEYQVHLPPTFHAVAFYVMDEDALSRDDVIGKVCLTRDTIASHPKGFSGWAHLTEVDPDEEVQGEIHLRLEVWPGARACRLRCSVLEARDLAPKDRNGTSDPFVRVRYKGRTRETSIVKKSCYPRWNETFEFELQEGAMEALCVEAWDWDLVSRNDFLGKVVIDVQRLRVVQQEEGWFRLQPDQSKSRRHDEGNLGSLQLEVRLRDETVLPSSYYQPLVHLLCHEVKLGMQGPGQLIPLIEETTSTECRQDVATNLLKLFLGQGLAKDFLDLLFQLELSRTSETNTLFRSNSLASKSMESFLKVAGMQYLHGVLGPIINKVFEEKKYVELDPSKVEVKDVGCSGLHRPQTEAEVLEQSAQTLRAHLGALLSALSRSVRACPAVVRATFRQLFRRVRERFPGAQHENVPFIAVTSFLCLRFFSPAIMSPKLFHLRERHADARTSRTLLLLAKAVQNVGNMDTPASRAKEAWMEPLQPTVRQGVAQLKDFITKLVDIEEKDELDLQRTLSLQAPPVKEGPLFIHRTKGKGPLMSSSFKKLYFSLTTEALSFAKTPSSKCVNELNQWLSALRKVSINNTGLLGSYHPGVFRGDKWSCCHQKEKTGQGCDKTRSRVTLQEWNDPLDHDLEAQLIYRHLLGVEAMLWERHRELSGGAEAGTVPTSPGKGRSCPPGTCSWPEPPPEMHGWGPRAHSQGPSRLPLPTVPEDSLARLLRVLQDLREAHSSSPAGSPPSEPNCLLELQT

Target ORF information:

RefSeq Version	XM_017011662.1
Organism	Homo sapiens(human)
Definition	Homo sapiens RAS p21 protein activator 4 (RASA4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017011662.1

ORF Insert Sequence:

ATGGCCAAGC GCAGCTCGCT GTACATCCGC ATCGTGGAGG GGAAGAACCT TCCCGCCAAG 
GACATCACTG GCAGCAGCGA CCCCTACTGC ATCGTGAAGG TGGACAATGA GCCCATCATC 
AGGACAGCCA CAGTGTGGAA GACCCTGTGC CCCTTCTGGG GTGAGGAGTA CCAAGTGCAC 
CTGCCGCCCA CCTTCCACGC TGTGGCTTTC TATGTCATGG ATGAGGATGC CCTCAGCCGG 
GACGACGTTA TCGGAAAGGT CTGCCTTACA AGGGACACCA TAGCCTCTCA CCCTAAGGGT 
TTCAGCGGGT GGGCCCACCT GACGGAGGTC GACCCCGATG AGGAGGTGCA GGGCGAGATC 
CACCTGCGGC TGGAAGTGTG GCCAGGGGCC CGGGCCTGCC GGCTACGCTG CTCTGTGCTG 
GAGGCCAGGG ATCTGGCCCC AAAGGACCGC AATGGCACAT CTGACCCCTT CGTCCGAGTG 
CGCTACAAGG GCCGGACACG GGAGACCTCG ATCGTGAAGA AGTCATGCTA CCCACGCTGG 
AATGAGACGT TTGAATTTGA GCTGCAGGAG GGGGCCATGG AGGCGCTGTG CGTGGAGGCC 
TGGGACTGGG ACCTTGTCAG CCGAAACGAC TTCCTGGGCA AAGTGGTGAT TGATGTCCAG 
AGACTGCGGG TGGTGCAGCA GGAGGAGGGC TGGTTCCGGC TGCAGCCCGA CCAGTCCAAG 
AGCCGGCGGC ATGACGAGGG CAACCTGGGC TCCTTGCAGC TGGAGGTGCG GCTGCGGGAC 
GAGACGGTGC TGCCCTCCAG CTACTACCAG CCACTGGTGC ACCTGCTGTG CCACGAGGTC 
AAGCTGGGCA TGCAGGGCCC AGGGCAGCTG ATCCCACTCA TCGAGGAGAC AACCAGCACC 
GAGTGTCGCC AGGACGTGGC CACGAACCTG CTCAAGCTCT TCCTGGGGCA GGGGCTGGCC 
AAGGACTTCC TGGACCTGCT CTTCCAGCTG GAGCTGAGTC GCACCAGTGA GACCAACACC 
CTGTTCCGGA GCAACTCTCT GGCCTCAAAG TCCATGGAGT CTTTTCTGAA GGTGGCCGGG 
ATGCAGTACC TGCACGGCGT CCTGGGCCCC ATCATCAACA AGGTGTTTGA GGAGAAGAAG 
TACGTGGAGC TGGACCCCAG CAAAGTGGAA GTTAAGGATG TAGGGTGCTC CGGGCTGCAC 
CGCCCGCAGA CCGAGGCCGA GGTGCTGGAG CAGAGCGCGC AGACGCTGCG CGCCCACCTG 
GGGGCCCTGC TGAGCGCGCT CAGCCGCTCG GTTCGCGCGT GCCCCGCCGT GGTGCGCGCC 
ACCTTCCGCC AGCTCTTCCG GCGCGTGCGC GAGCGCTTCC CCGGCGCCCA GCACGAGAAT 
GTACCGTTCA TCGCCGTCAC CAGCTTCCTG TGCCTGCGCT TCTTCTCTCC CGCCATCATG 
TCGCCCAAGC TCTTCCACCT GCGGGAGCGC CACGCGGACG CCCGCACCAG CCGCACCCTG 
CTCCTGTTGG CCAAGGCAGT CCAGAACGTG GGCAACATGG ACACGCCGGC TTCCAGGGCC 
AAGGAGGCTT GGATGGAGCC GCTGCAGCCC ACCGTGCGCC AGGGCGTGGC GCAGCTGAAG 
GACTTCATCA CCAAGCTCGT GGACATCGAG GAGAAGGACG AGCTGGACCT GCAGCGGACG 
CTGAGTTTGC AGGCGCCACC TGTGAAGGAG GGGCCACTCT TCATCCACAG GACCAAGGGC 
AAGGGCCCCC TCATGTCCTC CTCCTTCAAG AAGCTCTACT TCTCCCTCAC TACCGAGGCC 
CTCAGCTTCG CGAAGACGCC CAGCTCCAAG TGTGTGAATG AGCTTAACCA GTGGCTGTCT 
GCGCTGCGGA AGGTGAGCAT CAACAACACC GGACTGCTGG GCTCCTACCA CCCTGGCGTC 
TTCCGTGGGG ACAAGTGGAG CTGCTGCCAC CAAAAAGAGA AGACAGGTCA GGGCTGCGAT 
AAGACCCGGT CACGGGTGAC CCTGCAGGAG TGGAATGACC CTCTTGACCA TGACCTTGAG 
GCCCAGCTCA TCTACCGGCA CCTGCTGGGC GTGGAGGCCA TGCTGTGGGA GAGGCACCGG 
GAGCTGAGCG GGGGCGCAGA GGCAGGCACG GTGCCCACGA GCCCTGGCAA AGGTAGGTCT 
TGCCCACCTG GGACTTGCTC GTGGCCCGAG CCCCCTCCAG AGATGCACGG GTGGGGTCCT 
CGGGCTCACT CCCAGGGGCC CTCACGGCTG CCTCTGCCCA CAGTCCCCGA GGACTCATTG 
GCCCGGCTGC TCCGGGTGCT GCAGGACCTC CGCGAGGCCC ATAGCTCCAG CCCGGCCGGC 
TCCCCACCCT CAGAGCCCAA CTGCCTCCTG GAGCTGCAGA CGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASA4 ( XM_017011662.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016867151.1 Homo sapiens RAS p21 protein activator 4 (RASA4), transcript variant X2, mRNA.

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CloneID	OHu96999
Clone ID Related Accession (Same CDS sequence)	XM_017011662.1 , XM_017011662.2
Accession Version	XM_017011662.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2385bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ras GTPase-activating protein 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017011662.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGC GCAGCTCGCT GTACATCCGC ATCGTGGAGG GGAAGAACCT TCCCGCCAAG 
GACATCACTG GCAGCAGCGA CCCCTACTGC ATCGTGAAGG TGGACAATGA GCCCATCATC 
AGGACAGCCA CAGTGTGGAA GACCCTGTGC CCCTTCTGGG GTGAGGAGTA CCAAGTGCAC 
CTGCCGCCCA CCTTCCACGC TGTGGCTTTC TATGTCATGG ATGAGGATGC CCTCAGCCGG 
GACGACGTTA TCGGAAAGGT CTGCCTTACA AGGGACACCA TAGCCTCTCA CCCTAAGGGT 
TTCAGCGGGT GGGCCCACCT GACGGAGGTC GACCCCGATG AGGAGGTGCA GGGCGAGATC 
CACCTGCGGC TGGAAGTGTG GCCAGGGGCC CGGGCCTGCC GGCTACGCTG CTCTGTGCTG 
GAGGCCAGGG ATCTGGCCCC AAAGGACCGC AATGGCACAT CTGACCCCTT CGTCCGAGTG 
CGCTACAAGG GCCGGACACG GGAGACCTCG ATCGTGAAGA AGTCATGCTA CCCACGCTGG 
AATGAGACGT TTGAATTTGA GCTGCAGGAG GGGGCCATGG AGGCGCTGTG CGTGGAGGCC 
TGGGACTGGG ACCTTGTCAG CCGAAACGAC TTCCTGGGCA AAGTGGTGAT TGATGTCCAG 
AGACTGCGGG TGGTGCAGCA GGAGGAGGGC TGGTTCCGGC TGCAGCCCGA CCAGTCCAAG 
AGCCGGCGGC ATGACGAGGG CAACCTGGGC TCCTTGCAGC TGGAGGTGCG GCTGCGGGAC 
GAGACGGTGC TGCCCTCCAG CTACTACCAG CCACTGGTGC ACCTGCTGTG CCACGAGGTC 
AAGCTGGGCA TGCAGGGCCC AGGGCAGCTG ATCCCACTCA TCGAGGAGAC AACCAGCACC 
GAGTGTCGCC AGGACGTGGC CACGAACCTG CTCAAGCTCT TCCTGGGGCA GGGGCTGGCC 
AAGGACTTCC TGGACCTGCT CTTCCAGCTG GAGCTGAGTC GCACCAGTGA GACCAACACC 
CTGTTCCGGA GCAACTCTCT GGCCTCAAAG TCCATGGAGT CTTTTCTGAA GGTGGCCGGG 
ATGCAGTACC TGCACGGCGT CCTGGGCCCC ATCATCAACA AGGTGTTTGA GGAGAAGAAG 
TACGTGGAGC TGGACCCCAG CAAAGTGGAA GTTAAGGATG TAGGGTGCTC CGGGCTGCAC 
CGCCCGCAGA CCGAGGCCGA GGTGCTGGAG CAGAGCGCGC AGACGCTGCG CGCCCACCTG 
GGGGCCCTGC TGAGCGCGCT CAGCCGCTCG GTTCGCGCGT GCCCCGCCGT GGTGCGCGCC 
ACCTTCCGCC AGCTCTTCCG GCGCGTGCGC GAGCGCTTCC CCGGCGCCCA GCACGAGAAT 
GTACCGTTCA TCGCCGTCAC CAGCTTCCTG TGCCTGCGCT TCTTCTCTCC CGCCATCATG 
TCGCCCAAGC TCTTCCACCT GCGGGAGCGC CACGCGGACG CCCGCACCAG CCGCACCCTG 
CTCCTGTTGG CCAAGGCAGT CCAGAACGTG GGCAACATGG ACACGCCGGC TTCCAGGGCC 
AAGGAGGCTT GGATGGAGCC GCTGCAGCCC ACCGTGCGCC AGGGCGTGGC GCAGCTGAAG 
GACTTCATCA CCAAGCTCGT GGACATCGAG GAGAAGGACG AGCTGGACCT GCAGCGGACG 
CTGAGTTTGC AGGCGCCACC TGTGAAGGAG GGGCCACTCT TCATCCACAG GACCAAGGGC 
AAGGGCCCCC TCATGTCCTC CTCCTTCAAG AAGCTCTACT TCTCCCTCAC TACCGAGGCC 
CTCAGCTTCG CGAAGACGCC CAGCTCCAAG TGTGTGAATG AGCTTAACCA GTGGCTGTCT 
GCGCTGCGGA AGGTGAGCAT CAACAACACC GGACTGCTGG GCTCCTACCA CCCTGGCGTC 
TTCCGTGGGG ACAAGTGGAG CTGCTGCCAC CAAAAAGAGA AGACAGGTCA GGGCTGCGAT 
AAGACCCGGT CACGGGTGAC CCTGCAGGAG TGGAATGACC CTCTTGACCA TGACCTTGAG 
GCCCAGCTCA TCTACCGGCA CCTGCTGGGC GTGGAGGCCA TGCTGTGGGA GAGGCACCGG 
GAGCTGAGCG GGGGCGCAGA GGCAGGCACG GTGCCCACGA GCCCTGGCAA AGGTAGGTCT 
TGCCCACCTG GGACTTGCTC GTGGCCCGAG CCCCCTCCAG AGATGCACGG GTGGGGTCCT 
CGGGCTCACT CCCAGGGGCC CTCACGGCTG CCTCTGCCCA CAGTCCCCGA GGACTCATTG 
GCCCGGCTGC TCCGGGTGCT GCAGGACCTC CGCGAGGCCC ATAGCTCCAG CCCGGCCGGC 
TCCCCACCCT CAGAGCCCAA CTGCCTCCTG GAGCTGCAGA CGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016867151.1
CDS	69..2453
Translation	MAKRSSLYIRIVEGKNLPAKDITGSSDPYCIVKVDNEPIIRTATVWKTLCPFWGEEYQVHLPPTFHAVAFYVMDEDALSRDDVIGKVCLTRDTIASHPKGFSGWAHLTEVDPDEEVQGEIHLRLEVWPGARACRLRCSVLEARDLAPKDRNGTSDPFVRVRYKGRTRETSIVKKSCYPRWNETFEFELQEGAMEALCVEAWDWDLVSRNDFLGKVVIDVQRLRVVQQEEGWFRLQPDQSKSRRHDEGNLGSLQLEVRLRDETVLPSSYYQPLVHLLCHEVKLGMQGPGQLIPLIEETTSTECRQDVATNLLKLFLGQGLAKDFLDLLFQLELSRTSETNTLFRSNSLASKSMESFLKVAGMQYLHGVLGPIINKVFEEKKYVELDPSKVEVKDVGCSGLHRPQTEAEVLEQSAQTLRAHLGALLSALSRSVRACPAVVRATFRQLFRRVRERFPGAQHENVPFIAVTSFLCLRFFSPAIMSPKLFHLRERHADARTSRTLLLLAKAVQNVGNMDTPASRAKEAWMEPLQPTVRQGVAQLKDFITKLVDIEEKDELDLQRTLSLQAPPVKEGPLFIHRTKGKGPLMSSSFKKLYFSLTTEALSFAKTPSSKCVNELNQWLSALRKVSINNTGLLGSYHPGVFRGDKWSCCHQKEKTGQGCDKTRSRVTLQEWNDPLDHDLEAQLIYRHLLGVEAMLWERHRELSGGAEAGTVPTSPGKGRSCPPGTCSWPEPPPEMHGWGPRAHSQGPSRLPLPTVPEDSLARLLRVLQDLREAHSSSPAGSPPSEPNCLLELQT

Target ORF information:

RefSeq Version	XM_017011662.2
Organism	Homo sapiens(human)
Definition	Homo sapiens RAS p21 protein activator 4 (RASA4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017011662.2

ORF Insert Sequence:

ATGGCCAAGC GCAGCTCGCT GTACATCCGC ATCGTGGAGG GGAAGAACCT TCCCGCCAAG 
GACATCACTG GCAGCAGCGA CCCCTACTGC ATCGTGAAGG TGGACAATGA GCCCATCATC 
AGGACAGCCA CAGTGTGGAA GACCCTGTGC CCCTTCTGGG GTGAGGAGTA CCAAGTGCAC 
CTGCCGCCCA CCTTCCACGC TGTGGCTTTC TATGTCATGG ATGAGGATGC CCTCAGCCGG 
GACGACGTTA TCGGAAAGGT CTGCCTTACA AGGGACACCA TAGCCTCTCA CCCTAAGGGT 
TTCAGCGGGT GGGCCCACCT GACGGAGGTC GACCCCGATG AGGAGGTGCA GGGCGAGATC 
CACCTGCGGC TGGAAGTGTG GCCAGGGGCC CGGGCCTGCC GGCTACGCTG CTCTGTGCTG 
GAGGCCAGGG ATCTGGCCCC AAAGGACCGC AATGGCACAT CTGACCCCTT CGTCCGAGTG 
CGCTACAAGG GCCGGACACG GGAGACCTCG ATCGTGAAGA AGTCATGCTA CCCACGCTGG 
AATGAGACGT TTGAATTTGA GCTGCAGGAG GGGGCCATGG AGGCGCTGTG CGTGGAGGCC 
TGGGACTGGG ACCTTGTCAG CCGAAACGAC TTCCTGGGCA AAGTGGTGAT TGATGTCCAG 
AGACTGCGGG TGGTGCAGCA GGAGGAGGGC TGGTTCCGGC TGCAGCCCGA CCAGTCCAAG 
AGCCGGCGGC ATGACGAGGG CAACCTGGGC TCCTTGCAGC TGGAGGTGCG GCTGCGGGAC 
GAGACGGTGC TGCCCTCCAG CTACTACCAG CCACTGGTGC ACCTGCTGTG CCACGAGGTC 
AAGCTGGGCA TGCAGGGCCC AGGGCAGCTG ATCCCACTCA TCGAGGAGAC AACCAGCACC 
GAGTGTCGCC AGGACGTGGC CACGAACCTG CTCAAGCTCT TCCTGGGGCA GGGGCTGGCC 
AAGGACTTCC TGGACCTGCT CTTCCAGCTG GAGCTGAGTC GCACCAGTGA GACCAACACC 
CTGTTCCGGA GCAACTCTCT GGCCTCAAAG TCCATGGAGT CTTTTCTGAA GGTGGCCGGG 
ATGCAGTACC TGCACGGCGT CCTGGGCCCC ATCATCAACA AGGTGTTTGA GGAGAAGAAG 
TACGTGGAGC TGGACCCCAG CAAAGTGGAA GTTAAGGATG TAGGGTGCTC CGGGCTGCAC 
CGCCCGCAGA CCGAGGCCGA GGTGCTGGAG CAGAGCGCGC AGACGCTGCG CGCCCACCTG 
GGGGCCCTGC TGAGCGCGCT CAGCCGCTCG GTTCGCGCGT GCCCCGCCGT GGTGCGCGCC 
ACCTTCCGCC AGCTCTTCCG GCGCGTGCGC GAGCGCTTCC CCGGCGCCCA GCACGAGAAT 
GTACCGTTCA TCGCCGTCAC CAGCTTCCTG TGCCTGCGCT TCTTCTCTCC CGCCATCATG 
TCGCCCAAGC TCTTCCACCT GCGGGAGCGC CACGCGGACG CCCGCACCAG CCGCACCCTG 
CTCCTGTTGG CCAAGGCAGT CCAGAACGTG GGCAACATGG ACACGCCGGC TTCCAGGGCC 
AAGGAGGCTT GGATGGAGCC GCTGCAGCCC ACCGTGCGCC AGGGCGTGGC GCAGCTGAAG 
GACTTCATCA CCAAGCTCGT GGACATCGAG GAGAAGGACG AGCTGGACCT GCAGCGGACG 
CTGAGTTTGC AGGCGCCACC TGTGAAGGAG GGGCCACTCT TCATCCACAG GACCAAGGGC 
AAGGGCCCCC TCATGTCCTC CTCCTTCAAG AAGCTCTACT TCTCCCTCAC TACCGAGGCC 
CTCAGCTTCG CGAAGACGCC CAGCTCCAAG TGTGTGAATG AGCTTAACCA GTGGCTGTCT 
GCGCTGCGGA AGGTGAGCAT CAACAACACC GGACTGCTGG GCTCCTACCA CCCTGGCGTC 
TTCCGTGGGG ACAAGTGGAG CTGCTGCCAC CAAAAAGAGA AGACAGGTCA GGGCTGCGAT 
AAGACCCGGT CACGGGTGAC CCTGCAGGAG TGGAATGACC CTCTTGACCA TGACCTTGAG 
GCCCAGCTCA TCTACCGGCA CCTGCTGGGC GTGGAGGCCA TGCTGTGGGA GAGGCACCGG 
GAGCTGAGCG GGGGCGCAGA GGCAGGCACG GTGCCCACGA GCCCTGGCAA AGGTAGGTCT 
TGCCCACCTG GGACTTGCTC GTGGCCCGAG CCCCCTCCAG AGATGCACGG GTGGGGTCCT 
CGGGCTCACT CCCAGGGGCC CTCACGGCTG CCTCTGCCCA CAGTCCCCGA GGACTCATTG 
GCCCGGCTGC TCCGGGTGCT GCAGGACCTC CGCGAGGCCC ATAGCTCCAG CCCGGCCGGC 
TCCCCACCCT CAGAGCCCAA CTGCCTCCTG GAGCTGCAGA CGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

RASA4 undergoes DNA hypermethylation in resistant juvenile myelomonocytic leukemia.
Epigenetics9(9)1252-60(2014 Sep)
Poetsch AR,Lipka DB,Witte T,Claus R,N?llke P,Zucknick M,Olk-Batz C,Fluhr S,Dworzak M,De Moerloose B,Star? J,Zecca M,Hasle H,Schmugge M,van den Heuvel-Eibrink MM,Locatelli F,Niemeyer CM,Flotho C,Plass C

Ca2+-dependent monomer and dimer formation switches CAPRI Protein between Ras GTPase-activating protein (GAP) and RapGAP activities.
The Journal of biological chemistry286(22)19905-16(2011 Jun)
Dai Y,Walker SA,de Vet E,Cook S,Welch HC,Lockyer PJ

CAPRI and RASAL impose different modes of information processing on Ras due to contrasting temporal filtering of Ca2+.
The Journal of cell biology170(2)183-90(2005 Jul)
Liu Q,Walker SA,Gao D,Taylor JA,Dai YF,Arkell RS,Bootman MD,Roderick HL,Cullen PJ,Lockyer PJ

Phospholipase Cgamma activates Ras on the Golgi apparatus by means of RasGRP1.
Nature424(6949)694-8(2003 Aug)
Bivona TG,P?rez De Castro I,Ahearn IM,Grana TM,Chiu VK,Lockyer PJ,Cullen PJ,Pellicer A,Cox AD,Philips MR

Distinct phosphoinositide binding specificity of the GAP1 family proteins: characterization of the pleckstrin homology domains of MRASAL and KIAA0538.
Biochemical and biophysical research communications288(1)87-90(2001 Oct)
Minagawa T,Fukuda M,Mikoshiba K

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RASA4 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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