CELA3A cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	CELA3A
Entrez Gene ID	10136
Full Name	chymotrypsin like elastase family member 3A
Synonyms	ELA3,ELA3A
General protein information	Preferred Names chymotrypsin like elastase family member 3A Names chymotrypsin-like elastase family member 3A elastase 1 elastase 3A, pancreatic elastase IIIA elastase-3A protease E
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1p36.12
Summary	Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, elastase 3A has little elastolytic activity. Like most of the human elastases, elastase 3A is secreted from the pancreas as a zymogen and, like other serine proteases such as trypsin, chymotrypsin and kallikrein, it has a digestive function in the intestine. Elastase 3A preferentially cleaves proteins after alanine residues. Elastase 3A may also function in the intestinal transport and metabolism of cholesterol. Both elastase 3A and elastase 3B have been referred to as protease E and as elastase 1. [provided by RefSeq, Jul 2008].

mRNA and Protein(s)

mRNA	Protein	Name
NM_005747.5	NP_005738.4	chymotrypsin-like elastase family member 3A preproprotein
NM_005747.4	NP_005738.4	chymotrypsin-like elastase family member 3A preproprotein

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	CELA3A	XP_003307905.1
Homo sapiens (human)	CELA3A	NP_005738.4
Mus musculus (house mouse)	Gm13011	NP_001119790.1
Xenopus tropicalis (tropical clawed frog)	ela3b	NP_001011421.1

Gene Ontology
Bibliography

Related articles in PubMed

Genetic Analysis of Human Chymotrypsin-Like Elastases 3A and 3B (CELA3A and CELA3B) to Assess the Role of Complex Formation between Proelastases and Procarboxypeptidases in Chronic Pancreatitis.
P?rniczky A, Hegyi E, T?th AZ, Sz?cs ?, Szentesi A, Vincze ?, Izb?ki F, N?meth BC, Hegyi P, Sahin-T?th M
International journal of molecular sciences17(12)(2016 Dec)

Complex Formation of Human Proelastases with Procarboxypeptidases A1 and A2.
Szab? A, Pilsak C, Bence M, Witt H, Sahin-T?th M
The Journal of biological chemistry291(34)17706-16(2016 08)

Pancreatic elastase IIIA and its variants are expressed in pancreatic carcinoma cells.
Shimada S, Yamaguchi K, Takahashi M, Ogawa M
International journal of molecular medicine10(5)599-603(2002 Nov)

Molecular cloning of complementary DNA encoding one of the human pancreatic protease E isozymes.
Shirasu Y, Takemura K, Yoshida H, Sato Y, Iijima H, Shimada Y, Mikayama T, Ozawa T, Ikeda N, Ishida A
Journal of biochemistry104(2)259-64(1988 Aug)

Identification of a novel class of elastase isozyme, human pancreatic elastase III, by cDNA and genomic gene cloning.
Tani T, Ohsumi J, Mita K, Takiguchi Y
The Journal of biological chemistry263(3)1231-9(1988 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

variants affecting amino-acid position 241 in CELA3A and CELA3B are not associated with chronic pancreatitis, indicating that changes in complex formation between proelastases and procarboxypeptidases do not alter pancreatitis risk.
Title: Genetic Analysis of Human Chymotrypsin-Like Elastases 3A and 3B (CELA3A and CELA3B) to Assess the Role of Complex Formation between Proelastases and Procarboxypeptidases in Chronic Pancreatitis.

Complex Formation of Human Proelastases with Procarboxypeptidases A1 and A2.
Title: Complex Formation of Human Proelastases with Procarboxypeptidases A1 and A2.

expression of PE IIIA and its splicing variants in pancreatic carcinoma cells
Title: Pancreatic elastase IIIA and its variants are expressed in pancreatic carcinoma cells.

The following CELA3A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CELA3A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu05020	NM_005747.5 Latest version!	Homo sapiens chymotrypsin like elastase 3A (CELA3A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$216.30 $309.00
View Old Accession Versions >>
OHu05020	NM_005747.4	Homo sapiens chymotrypsin like elastase 3A (CELA3A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$216.30 $309.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CELA3A ( NM_005747.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_005738.4 Homo sapiens chymotrypsin like elastase 3A (CELA3A), mRNA.

Price & Availability↑

CloneID	OHu05020
Clone ID Related Accession (Same CDS sequence)	NM_005747.5 , NM_005747.4
Accession Version	NM_005747.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 813bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-04
Organism	Homo sapiens(human)
Product	chymotrypsin-like elastase family member 3A preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC015103.1, AW583482.1 and AK225620.1. On May 17, 2019 this sequence version replaced NM_005747.4. ##Evidence-Data-START## Transcript exon combination :: AK225620.1, BC007028.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000290122.8/ ENSP00000290122.3 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGATGCTCC GGCTGCTCAG TTCCCTCCTC CTTGTGGCCG TTGCCTCAGG CTATGGCCCA 
CCTTCCTCTC ACTCTTCCAG CCGCGTTGTC CATGGTGAGG ATGCGGTCCC CTACAGCTGG 
CCCTGGCAGG TTTCCCTGCA GTATGAGAAA AGTGGAAGCT TCTACCACAC GTGTGGCGGT 
AGCCTCATCG CCCCCGATTG GGTTGTGACT GCCGGCCACT GCATCTCGAG GGATCTGACC 
TACCAGGTGG TGTTGGGTGA GTACAACCTT GCTGTGAAGG AGGGCCCCGA GCAGGTGATC 
CCCATCAACT CTGAGGAGCT GTTTGTGCAT CCACTCTGGA ACCGCTCGTG TGTGGCCTGT 
GGCAATGACA TCGCCCTCAT CAAGCTCTCA CGCAGCGCCC AGCTGGGAGA TGCCGTCCAG 
CTCGCCTCAC TCCCTCCCGC TGGTGACATC CTTCCCAACA AGACACCCTG CTACATCACC 
GGCTGGGGCC GTCTCTATAC CAATGGGCCA CTCCCAGACA AGCTGCAGCA GGCCCGGCTG 
CCCGTGGTGG ACTATAAGCA CTGCTCCAGG TGGAACTGGT GGGGTTCCAC CGTGAAGAAA 
ACCATGGTGT GTGCTGGAGG GTACATCCGC TCCGGCTGCA ACGGTGACTC TGGAGGACCC 
CTCAACTGCC CCACAGAGGA TGGTGGCTGG CAGGTCCACG GTGTGACCAG CTTTGTTTCT 
GCCTTTGGCT GCAACTTCAT CTGGAAGCCC ACGGTGTTCA CTCGAGTCTC CGCCTTCATC 
GACTGGATTG AGGAGACCAT AGCAAGCCAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005738.4
CDS	19..831
Translation	MMLRLLSSLLLVAVASGYGPPSSHSSSRVVHGEDAVPYSWPWQVSLQYEKSGSFYHTCGGSLIAPDWVVTAGHCISRDLTYQVVLGEYNLAVKEGPEQVIPINSEELFVHPLWNRSCVACGNDIALIKLSRSAQLGDAVQLASLPPAGDILPNKTPCYITGWGRLYTNGPLPDKLQQARLPVVDYKHCSRWNWWGSTVKKTMVCAGGYIRSGCNGDSGGPLNCPTEDGGWQVHGVTSFVSAFGCNFIWKPTVFTRVSAFIDWIEETIASH

Target ORF information:

RefSeq Version	NM_005747.5
Organism	Homo sapiens(human)
Definition	Homo sapiens chymotrypsin like elastase 3A (CELA3A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005747.5

ORF Insert Sequence:

ATGATGCTCC GGCTGCTCAG TTCCCTCCTC CTTGTGGCCG TTGCCTCAGG CTATGGCCCA 
CCTTCCTCTC ACTCTTCCAG CCGCGTTGTC CATGGTGAGG ATGCGGTCCC CTACAGCTGG 
CCCTGGCAGG TTTCCCTGCA GTATGAGAAA AGTGGAAGCT TCTACCACAC GTGTGGCGGT 
AGCCTCATCG CCCCCGATTG GGTTGTGACT GCCGGCCACT GCATCTCGAG GGATCTGACC 
TACCAGGTGG TGTTGGGTGA GTACAACCTT GCTGTGAAGG AGGGCCCCGA GCAGGTGATC 
CCCATCAACT CTGAGGAGCT GTTTGTGCAT CCACTCTGGA ACCGCTCGTG TGTGGCCTGT 
GGCAATGACA TCGCCCTCAT CAAGCTCTCA CGCAGCGCCC AGCTGGGAGA TGCCGTCCAG 
CTCGCCTCAC TCCCTCCCGC TGGTGACATC CTTCCCAACA AGACACCCTG CTACATCACC 
GGCTGGGGCC GTCTCTATAC CAATGGGCCA CTCCCAGACA AGCTGCAGCA GGCCCGGCTG 
CCCGTGGTGG ACTATAAGCA CTGCTCCAGG TGGAACTGGT GGGGTTCCAC CGTGAAGAAA 
ACCATGGTGT GTGCTGGAGG GTACATCCGC TCCGGCTGCA ACGGTGACTC TGGAGGACCC 
CTCAACTGCC CCACAGAGGA TGGTGGCTGG CAGGTCCACG GTGTGACCAG CTTTGTTTCT 
GCCTTTGGCT GCAACTTCAT CTGGAAGCCC ACGGTGTTCA CTCGAGTCTC CGCCTTCATC 
GACTGGATTG AGGAGACCAT AGCAAGCCAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CELA3A ( NM_005747.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_005738.4 Homo sapiens chymotrypsin like elastase 3A (CELA3A), mRNA.

Price & Availability↑

CloneID	OHu05020
Clone ID Related Accession (Same CDS sequence)	NM_005747.5 , NM_005747.4
Accession Version	NM_005747.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 813bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-10
Organism	Homo sapiens(human)
Product	chymotrypsin-like elastase family member 3A preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC015103.1, AW583482.1 and AK225620.1. On May 13, 2009 this sequence version replaced NM_005747.3. ##Evidence-Data-START## Transcript exon combination :: BC005918.1, BC015103.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATGCTCC GGCTGCTCAG TTCCCTCCTC CTTGTGGCCG TTGCCTCAGG CTATGGCCCA 
CCTTCCTCTC ACTCTTCCAG CCGCGTTGTC CATGGTGAGG ATGCGGTCCC CTACAGCTGG 
CCCTGGCAGG TTTCCCTGCA GTATGAGAAA AGTGGAAGCT TCTACCACAC GTGTGGCGGT 
AGCCTCATCG CCCCCGATTG GGTTGTGACT GCCGGCCACT GCATCTCGAG GGATCTGACC 
TACCAGGTGG TGTTGGGTGA GTACAACCTT GCTGTGAAGG AGGGCCCCGA GCAGGTGATC 
CCCATCAACT CTGAGGAGCT GTTTGTGCAT CCACTCTGGA ACCGCTCGTG TGTGGCCTGT 
GGCAATGACA TCGCCCTCAT CAAGCTCTCA CGCAGCGCCC AGCTGGGAGA TGCCGTCCAG 
CTCGCCTCAC TCCCTCCCGC TGGTGACATC CTTCCCAACA AGACACCCTG CTACATCACC 
GGCTGGGGCC GTCTCTATAC CAATGGGCCA CTCCCAGACA AGCTGCAGCA GGCCCGGCTG 
CCCGTGGTGG ACTATAAGCA CTGCTCCAGG TGGAACTGGT GGGGTTCCAC CGTGAAGAAA 
ACCATGGTGT GTGCTGGAGG GTACATCCGC TCCGGCTGCA ACGGTGACTC TGGAGGACCC 
CTCAACTGCC CCACAGAGGA TGGTGGCTGG CAGGTCCACG GTGTGACCAG CTTTGTTTCT 
GCCTTTGGCT GCAACTTCAT CTGGAAGCCC ACGGTGTTCA CTCGAGTCTC CGCCTTCATC 
GACTGGATTG AGGAGACCAT AGCAAGCCAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005738.4
CDS	20..832
Translation	MMLRLLSSLLLVAVASGYGPPSSHSSSRVVHGEDAVPYSWPWQVSLQYEKSGSFYHTCGGSLIAPDWVVTAGHCISRDLTYQVVLGEYNLAVKEGPEQVIPINSEELFVHPLWNRSCVACGNDIALIKLSRSAQLGDAVQLASLPPAGDILPNKTPCYITGWGRLYTNGPLPDKLQQARLPVVDYKHCSRWNWWGSTVKKTMVCAGGYIRSGCNGDSGGPLNCPTEDGGWQVHGVTSFVSAFGCNFIWKPTVFTRVSAFIDWIEETIASH

Target ORF information:

RefSeq Version	NM_005747.4
Organism	Homo sapiens(human)
Definition	Homo sapiens chymotrypsin like elastase 3A (CELA3A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005747.4

ORF Insert Sequence:

ATGATGCTCC GGCTGCTCAG TTCCCTCCTC CTTGTGGCCG TTGCCTCAGG CTATGGCCCA 
CCTTCCTCTC ACTCTTCCAG CCGCGTTGTC CATGGTGAGG ATGCGGTCCC CTACAGCTGG 
CCCTGGCAGG TTTCCCTGCA GTATGAGAAA AGTGGAAGCT TCTACCACAC GTGTGGCGGT 
AGCCTCATCG CCCCCGATTG GGTTGTGACT GCCGGCCACT GCATCTCGAG GGATCTGACC 
TACCAGGTGG TGTTGGGTGA GTACAACCTT GCTGTGAAGG AGGGCCCCGA GCAGGTGATC 
CCCATCAACT CTGAGGAGCT GTTTGTGCAT CCACTCTGGA ACCGCTCGTG TGTGGCCTGT 
GGCAATGACA TCGCCCTCAT CAAGCTCTCA CGCAGCGCCC AGCTGGGAGA TGCCGTCCAG 
CTCGCCTCAC TCCCTCCCGC TGGTGACATC CTTCCCAACA AGACACCCTG CTACATCACC 
GGCTGGGGCC GTCTCTATAC CAATGGGCCA CTCCCAGACA AGCTGCAGCA GGCCCGGCTG 
CCCGTGGTGG ACTATAAGCA CTGCTCCAGG TGGAACTGGT GGGGTTCCAC CGTGAAGAAA 
ACCATGGTGT GTGCTGGAGG GTACATCCGC TCCGGCTGCA ACGGTGACTC TGGAGGACCC 
CTCAACTGCC CCACAGAGGA TGGTGGCTGG CAGGTCCACG GTGTGACCAG CTTTGTTTCT 
GCCTTTGGCT GCAACTTCAT CTGGAAGCCC ACGGTGTTCA CTCGAGTCTC CGCCTTCATC 
GACTGGATTG AGGAGACCAT AGCAAGCCAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetic Analysis of Human Chymotrypsin-Like Elastases 3A and 3B (CELA3A and CELA3B) to Assess the Role of Complex Formation between Proelastases and Procarboxypeptidases in Chronic Pancreatitis.
International journal of molecular sciences17(12)(2016 Dec)
P?rniczky A,Hegyi E,T?th AZ,Sz?cs ?,Szentesi A,Vincze ?,Izb?ki F,N?meth BC,Hegyi P,Sahin-T?th M

Complex Formation of Human Proelastases with Procarboxypeptidases A1 and A2.
The Journal of biological chemistry291(34)17706-16(2016 08)
Szab? A,Pilsak C,Bence M,Witt H,Sahin-T?th M

Pancreatic elastase IIIA and its variants are expressed in pancreatic carcinoma cells.
International journal of molecular medicine10(5)599-603(2002 Nov)
Shimada S,Yamaguchi K,Takahashi M,Ogawa M

Molecular cloning of complementary DNA encoding one of the human pancreatic protease E isozymes.
Journal of biochemistry104(2)259-64(1988 Aug)
Shirasu Y,Takemura K,Yoshida H,Sato Y,Iijima H,Shimada Y,Mikayama T,Ozawa T,Ikeda N,Ishida A

Identification of a novel class of elastase isozyme, human pancreatic elastase III, by cDNA and genomic gene cloning.
The Journal of biological chemistry263(3)1231-9(1988 Jan)
Tani T,Ohsumi J,Mita K,Takiguchi Y

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CELA3A cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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