ENAM cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	ENAM
Entrez Gene ID	10117
Full Name	enamelin
Synonyms	ADAI,AI1C,AIH2
General protein information	Preferred Names enamelin Names enamelin amelogenesis imperfecta 2, hypocalcification (autosomal dominant)
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 4 Map Location: 4q13.3
Summary	Dental enamel forms the outer cap of teeth and is the hardest substance found in vertebrates. This gene encodes the largest protein in the enamel matrix of developing teeth. The protein is involved in the mineralization and structural organization of enamel. Defects in this gene result in amelogenesis imperfect type 1C.[provided by RefSeq, Oct 2009].
Disorder MIM:	606585
Disorder Html:	Amelogenesis imperfecta, type IB, 104500 (3); Amelogenesis imperfecta, type IC, 204650 (3)

mRNA and Protein(s)

mRNA	Protein	Name
XM_006714056.2	XP_006714119.1	enamelin isoform X1
NM_001368133.1	NP_001355062.1	enamelin isoform 2
NM_031889.2	NP_114095.2	enamelin precursor
NM_031889.3	NP_114095.2	enamelin isoform 1 precursor
XM_006714056.3	XP_006714119.1	enamelin isoform X1
XM_006714056.4	XP_006714119.1	enamelin isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	ENAM	NP_114095.2
Pan troglodytes (chimpanzee)	ENAM	XP_526591.1
Mus musculus (house mouse)	Enam	NP_059496.1
Canis lupus familiaris (dog)	ENAM	XP_539305.3
Rattus norvegicus (Norway rat)	Enam	NP_001099471.1
Bos taurus (cattle)	ENAM	XP_002688385.1

Gene Ontology
Bibliography

Related articles in PubMed

Lack of association between ENAM gene polymorphism and dental caries in primary and permanent teeth in Czech children.
Borilova Linhartova P, Deissova T, Musilova K, Zackova L, Kukletova M, Kukla L, Izakovicova Holla L
Clinical oral investigations(2017 Nov)

The association of Enamelin, Lactoferrin, and Tumour necrosis factor alpha gene polymorphisms with high caries susceptibility in Chinese children under 4 years old.
Wang M, Qin M, Xia B
Archives of oral biology8075-81(2017 Aug)

Neanderthal and Denisova tooth protein variants in present-day humans.
Zanolli C, Hourset M, Esclassan R, Mollereau C
PloS one12(9)e0183802(2017)

Human enamel thickness and ENAM polymorphism.
Daubert DM, Kelley JL, Udod YG, Habor C, Kleist CG, Furman IK, Tikonov IN, Swanson WJ, Roberts FA
International journal of oral science8(2)93-7(2016 06)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Among the variants shared with modern humans, two are ancestral (common with apes) and one is the derived enamelin major variant, T648I (rs7671281), associated with a thinner enamel and specific to the Homo lineage.
Title: Neanderthal and Denisova tooth protein variants in present-day humans.

The study revealed the strong association between rs12640848 marker of ENAM gene and caries susceptibility in primary teeth in children from Poland.
Title: Association of ENAM gene single nucleotide polymorphisms with dental caries in Polish children.

Overrepresentation of the G allele of the enamelin marker was seen in the erosion group compared to the unaffected group.
Title: Enamel formation genes associated with dental erosive wear.

Screening of ENAM and LAMB3 genes was performed by direct sequencing of genomic DNA from blood samples.
Title: Novel ENAM and LAMB3 mutations in Chinese families with hypoplastic amelogenesis imperfecta.

findings provide useful information for the implication of ENAM gene polymorphism in autosomal-dominant/-recessive amelogenesis imperfecta.
Title: Enamelin/ameloblastin gene polymorphisms in autosomal amelogenesis imperfecta among Syrian families.

The following ENAM gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ENAM cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu107914	NM_001368133.1 Latest version!	Homo sapiens enamelin (ENAM), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OHu18574	NM_031889.3 Latest version!	Homo sapiens enamelin (ENAM), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$559.30 $799.00
OHu18574	XM_006714056.4 Latest version!	Homo sapiens enamelin (ENAM), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$559.30 $799.00
View Old Accession Versions >>
OHu18574	NM_031889.2	Homo sapiens enamelin (ENAM), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$559.30 $799.00
OHu18574	XM_006714056.2	Homo sapiens enamelin (ENAM), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$559.30 $799.00
OHu18574	XM_006714056.3	Homo sapiens enamelin (ENAM), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$559.30 $799.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ENAM ( NM_001368133.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001355062.1 Homo sapiens enamelin (ENAM), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu107914
Clone ID Related Accession (Same CDS sequence)	NM_001368133.1
Accession Version	NM_001368133.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2775bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-10
Organism	Homo sapiens(human)
Product	enamelin isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB088136.1, BC117310.1, AF125373.1 and AC009570.13. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DRR138522.182685.1, DB088136.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA1970526 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGTTTGAAC AAGATTTTGA AAAACCCAAA GAAGAAGATC CTCCTAAAGC AGAAAGTCCA 
GGCACAGAAC CCACAGCTAA TTCAACAGTC ACTGAGACGA ATTCTACCCA ACCAAATCCT 
AAAGGGAGTC AGGGAGGAAA TGACACCAGC CCCACAGGAA ACAGTACCCC AGGACTAAAC 
ACTGGGAACA ACCCTCCAGC TCAAAATGGG ATTGGCCCAC TCCCTGCAGT CAACGCTTCA 
GGCCAGGGAG GGCCAGGAAG TCAAATCCCA TGGAGACCAA GTCAGCCAAA TATTCGTGAA 
AATCATCCAT ATCCTAATAT AAGAAATTTT CCTTCAGGAA GACAGTGGTA TTTCACTGGT 
ACTGTCATGG GGCACAGACA GAATAGGCCT TTTTACAGAA ATCAACAAGT TCAAAGGGGT 
CCTCGGTGGA ACTTCTTTGC TTGGGAACGT AAACAAGTAG CTCGTCCAGG AAATCCAGTT 
TATCACAAAG CTTACCCTCC TACTTCAAGA GGCAATTATC CCAATTATGC AGGAAATCCA 
GCAAATCTCA GAAGAAAGCC TCAGGGGCCA AATAAACACC CTGTAGGAAC TACTGTTGCC 
CCACTGGGTC CCAAACCTGG CCCTGTTGTT CGCAATGAAA AAATCCAAAA TCCAAAGGAG 
AAGCCCCTGG GTCCAAAAGA ACAAATAATA GTTCCTACAA AGAATCCAAC CAGCCCCTGG 
AGAAACTCTC AACAGTATGA AGTTAATAAA TCAAATTATA AACTGCCTCA CTCTGAGGGT 
TATATGCCAG TCCCAAATTT TAATTCTGTT GATCAACATG AAAACTCCTA TTACCCAAGA 
GGAGATTCCA GAAAAGTCCC AAATTCTGAT GGACAAACCC AAAGCCAGAA TTTGCCCAAA 
GGGATTGTTT TAGGGTCAAG AAGGATGCCA TATGAATCAG AAACTAATCA GTCAGAATTA 
AAGCACAGCT CATATCAGCC TGCTGTATAC CCTGAGGAAA TCCCTTCTCC TGCAAAAGAA 
CATTTTCCTG CTGGAAGAAA TACTTGGGAC CACCAAGAAA TCTCTCCACC TTTTAAGGAA 
GATCCAGGGA GGCAAGAAGA ACATTTACCC CATCCTTCCC ATGGTTCTAG AGGAAGTGTT 
TTCTACCCTG AATATAACCC ATATGATCCC AGGGAAAACT CACCATACCT TAGAGGCAAT 
ACATGGGATG AGAGAGATGA TTCTCCCAAT ACTATGGGGC AAAAAGAAAG TCCACTCTAC 
CCCATAAATA CCCCAGACCA GAAGGAGATA GTCCCTTATA ATGAAGAGGA CCCAGTTGAT 
CCAACTGGAG ATGAAGTCTT TCCTGGACAA AATAGATGGG GTGAAGAGTT GAGCTTCAAA 
GGAGGCCCAA CAGTTAGGCA CTATGAAGGT GAACAATATA CCTCAAATCA GCCAAAGGAA 
TATCTTCCCT ATTCTTTAGA TAATCCATCA AAACCAAGGG AGGATTTTTA TTACAGTGAA 
TTTTACCCAT GGAGCCCGGA TGAGAATTTT CCATCATATA ATACAGCTTC TACTATGCCA 
CCACCTATAG AGAGCAGGGG CTACTACGTT AATAATGCCG CTGGACCAGA AGAAAGCACT 
CTATTTCCTT CACGGAATTC CTGGGACCAC AGGATACAAG CCCAAGGGCA GAGAGAAAGA 
AGGCCGTATT TTAACAGAAA TATCTGGGAT CAGGCAACAC ATTTACAAAA AGCCCCAGCT 
AGGCCACCAG ACCAGAAAGG TAACCAGCCC TATTACAGTA ACACCCCAGC TGGGCTTCAG 
AAAAATCCAA TATGGCATGA AGGTGAGAAT TTGAACTATG GCATGCAAAT AACTAGGATG 
AATTCTCCAG AGAGAGAACA TTCATCTTTC CCTAACTTCA TCCCACCAAG TTACCCATCA 
GGTCAAAAAG AAGCACATTT ATTTCACCTA AGCCAGAGAG GCTCTTGCTG TGCTGGTAGC 
TCCACAGGGC CCAAGGACAA TCCACTAGCT CTACAAGACT ACACTCCATC CTATGGTCTT 
GCACCTGGGG AGAACCAAGA CACCAGTCCT CTGTATACAG ACGGTAGTCA TACCAAGCAG 
ACAAGAGATA TCATCTCCCC AACAAGCATC CTACCAGGCC AAAGAAACAG CTCAGAGAAG 
AGGGAAAGCC AAAACCCTTT TAGAGATGAT GTGTCCACGC TGAGGAGGAA CACACCATGT 
TCTATAAAGA ATCAACTGGG CCAAAAGGAA ATTATGCCCT TTCCTGAAGC CAGTTCCCTT 
CAATCAAAGA ATACACCTTG TCTCAAAAAT GATCTTGGAG GAGATGGGAA CAACATTCTG 
GAACAAGTTT TTGAAGACAA CCAGCTCAAT GAAAGAACTG TTGACCTTAC TCCTGAGCAG 
CTTGTTATTG GTACACCTGA TGAAGGCTCC AATCCAGAAG GCATCCAAAG TCAAGTCCAA 
GAAAATGAGA GTGAGAGGCA ACAGCAAAGA CCATCTAACA TTCTGCATTT GCCATGCTTT 
GGCTCCAAAT TAGCAAAGCA TCACTCTTCC ACCACCGGAA CTCCATCTAG CGATGGAAGG 
CAAAGCCCAT TTGATGGGGA TTCAATTACG CCTACTGAAA ATCCTAACAC ATTGGTTGAG 
TTAGCTACTG AGGAACAATT TAAGAGTATA AATGTAGACC CACTTGATGC AGATGAACAC 
AGTCCATTTG AATTCCTTCA AAGAGGGACC AATGTACAGG ACCAGGTACA AGACTGCTTA 
CTACTTCAGG CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001355062.1
CDS	200..2974
Translation	MFEQDFEKPKEEDPPKAESPGTEPTANSTVTETNSTQPNPKGSQGGNDTSPTGNSTPGLNTGNNPPAQNGIGPLPAVNASGQGGPGSQIPWRPSQPNIRENHPYPNIRNFPSGRQWYFTGTVMGHRQNRPFYRNQQVQRGPRWNFFAWERKQVARPGNPVYHKAYPPTSRGNYPNYAGNPANLRRKPQGPNKHPVGTTVAPLGPKPGPVVRNEKIQNPKEKPLGPKEQIIVPTKNPTSPWRNSQQYEVNKSNYKLPHSEGYMPVPNFNSVDQHENSYYPRGDSRKVPNSDGQTQSQNLPKGIVLGSRRMPYESETNQSELKHSSYQPAVYPEEIPSPAKEHFPAGRNTWDHQEISPPFKEDPGRQEEHLPHPSHGSRGSVFYPEYNPYDPRENSPYLRGNTWDERDDSPNTMGQKESPLYPINTPDQKEIVPYNEEDPVDPTGDEVFPGQNRWGEELSFKGGPTVRHYEGEQYTSNQPKEYLPYSLDNPSKPREDFYYSEFYPWSPDENFPSYNTASTMPPPIESRGYYVNNAAGPEESTLFPSRNSWDHRIQAQGQRERRPYFNRNIWDQATHLQKAPARPPDQKGNQPYYSNTPAGLQKNPIWHEGENLNYGMQITRMNSPEREHSSFPNFIPPSYPSGQKEAHLFHLSQRGSCCAGSSTGPKDNPLALQDYTPSYGLAPGENQDTSPLYTDGSHTKQTRDIISPTSILPGQRNSSEKRESQNPFRDDVSTLRRNTPCSIKNQLGQKEIMPFPEASSLQSKNTPCLKNDLGGDGNNILEQVFEDNQLNERTVDLTPEQLVIGTPDEGSNPEGIQSQVQENESERQQQRPSNILHLPCFGSKLAKHHSSTTGTPSSDGRQSPFDGDSITPTENPNTLVELATEEQFKSINVDPLDADEHSPFEFLQRGTNVQDQVQDCLLLQA

Target ORF information:

RefSeq Version	NM_001368133.1
Organism	Homo sapiens(human)
Definition	Homo sapiens enamelin (ENAM), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001368133.1

ORF Insert Sequence:

ATGTTTGAAC AAGATTTTGA AAAACCCAAA GAAGAAGATC CTCCTAAAGC AGAAAGTCCA 
GGCACAGAAC CCACAGCTAA TTCAACAGTC ACTGAGACGA ATTCTACCCA ACCAAATCCT 
AAAGGGAGTC AGGGAGGAAA TGACACCAGC CCCACAGGAA ACAGTACCCC AGGACTAAAC 
ACTGGGAACA ACCCTCCAGC TCAAAATGGG ATTGGCCCAC TCCCTGCAGT CAACGCTTCA 
GGCCAGGGAG GGCCAGGAAG TCAAATCCCA TGGAGACCAA GTCAGCCAAA TATTCGTGAA 
AATCATCCAT ATCCTAATAT AAGAAATTTT CCTTCAGGAA GACAGTGGTA TTTCACTGGT 
ACTGTCATGG GGCACAGACA GAATAGGCCT TTTTACAGAA ATCAACAAGT TCAAAGGGGT 
CCTCGGTGGA ACTTCTTTGC TTGGGAACGT AAACAAGTAG CTCGTCCAGG AAATCCAGTT 
TATCACAAAG CTTACCCTCC TACTTCAAGA GGCAATTATC CCAATTATGC AGGAAATCCA 
GCAAATCTCA GAAGAAAGCC TCAGGGGCCA AATAAACACC CTGTAGGAAC TACTGTTGCC 
CCACTGGGTC CCAAACCTGG CCCTGTTGTT CGCAATGAAA AAATCCAAAA TCCAAAGGAG 
AAGCCCCTGG GTCCAAAAGA ACAAATAATA GTTCCTACAA AGAATCCAAC CAGCCCCTGG 
AGAAACTCTC AACAGTATGA AGTTAATAAA TCAAATTATA AACTGCCTCA CTCTGAGGGT 
TATATGCCAG TCCCAAATTT TAATTCTGTT GATCAACATG AAAACTCCTA TTACCCAAGA 
GGAGATTCCA GAAAAGTCCC AAATTCTGAT GGACAAACCC AAAGCCAGAA TTTGCCCAAA 
GGGATTGTTT TAGGGTCAAG AAGGATGCCA TATGAATCAG AAACTAATCA GTCAGAATTA 
AAGCACAGCT CATATCAGCC TGCTGTATAC CCTGAGGAAA TCCCTTCTCC TGCAAAAGAA 
CATTTTCCTG CTGGAAGAAA TACTTGGGAC CACCAAGAAA TCTCTCCACC TTTTAAGGAA 
GATCCAGGGA GGCAAGAAGA ACATTTACCC CATCCTTCCC ATGGTTCTAG AGGAAGTGTT 
TTCTACCCTG AATATAACCC ATATGATCCC AGGGAAAACT CACCATACCT TAGAGGCAAT 
ACATGGGATG AGAGAGATGA TTCTCCCAAT ACTATGGGGC AAAAAGAAAG TCCACTCTAC 
CCCATAAATA CCCCAGACCA GAAGGAGATA GTCCCTTATA ATGAAGAGGA CCCAGTTGAT 
CCAACTGGAG ATGAAGTCTT TCCTGGACAA AATAGATGGG GTGAAGAGTT GAGCTTCAAA 
GGAGGCCCAA CAGTTAGGCA CTATGAAGGT GAACAATATA CCTCAAATCA GCCAAAGGAA 
TATCTTCCCT ATTCTTTAGA TAATCCATCA AAACCAAGGG AGGATTTTTA TTACAGTGAA 
TTTTACCCAT GGAGCCCGGA TGAGAATTTT CCATCATATA ATACAGCTTC TACTATGCCA 
CCACCTATAG AGAGCAGGGG CTACTACGTT AATAATGCCG CTGGACCAGA AGAAAGCACT 
CTATTTCCTT CACGGAATTC CTGGGACCAC AGGATACAAG CCCAAGGGCA GAGAGAAAGA 
AGGCCGTATT TTAACAGAAA TATCTGGGAT CAGGCAACAC ATTTACAAAA AGCCCCAGCT 
AGGCCACCAG ACCAGAAAGG TAACCAGCCC TATTACAGTA ACACCCCAGC TGGGCTTCAG 
AAAAATCCAA TATGGCATGA AGGTGAGAAT TTGAACTATG GCATGCAAAT AACTAGGATG 
AATTCTCCAG AGAGAGAACA TTCATCTTTC CCTAACTTCA TCCCACCAAG TTACCCATCA 
GGTCAAAAAG AAGCACATTT ATTTCACCTA AGCCAGAGAG GCTCTTGCTG TGCTGGTAGC 
TCCACAGGGC CCAAGGACAA TCCACTAGCT CTACAAGACT ACACTCCATC CTATGGTCTT 
GCACCTGGGG AGAACCAAGA CACCAGTCCT CTGTATACAG ACGGTAGTCA TACCAAGCAG 
ACAAGAGATA TCATCTCCCC AACAAGCATC CTACCAGGCC AAAGAAACAG CTCAGAGAAG 
AGGGAAAGCC AAAACCCTTT TAGAGATGAT GTGTCCACGC TGAGGAGGAA CACACCATGT 
TCTATAAAGA ATCAACTGGG CCAAAAGGAA ATTATGCCCT TTCCTGAAGC CAGTTCCCTT 
CAATCAAAGA ATACACCTTG TCTCAAAAAT GATCTTGGAG GAGATGGGAA CAACATTCTG 
GAACAAGTTT TTGAAGACAA CCAGCTCAAT GAAAGAACTG TTGACCTTAC TCCTGAGCAG 
CTTGTTATTG GTACACCTGA TGAAGGCTCC AATCCAGAAG GCATCCAAAG TCAAGTCCAA 
GAAAATGAGA GTGAGAGGCA ACAGCAAAGA CCATCTAACA TTCTGCATTT GCCATGCTTT 
GGCTCCAAAT TAGCAAAGCA TCACTCTTCC ACCACCGGAA CTCCATCTAG CGATGGAAGG 
CAAAGCCCAT TTGATGGGGA TTCAATTACG CCTACTGAAA ATCCTAACAC ATTGGTTGAG 
TTAGCTACTG AGGAACAATT TAAGAGTATA AATGTAGACC CACTTGATGC AGATGAACAC 
AGTCCATTTG AATTCCTTCA AAGAGGGACC AATGTACAGG ACCAGGTACA AGACTGCTTA 
CTACTTCAGG CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ENAM ( NM_031889.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_114095.2 Homo sapiens enamelin (ENAM), mRNA.

Price & Availability↑

CloneID	OHu18574
Clone ID Related Accession (Same CDS sequence)	XM_006714056.2 , NM_031889.2 , NM_031889.3 , XM_006714056.3 , XM_006714056.4
Accession Version	NM_031889.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3429bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-17
Organism	Homo sapiens(human)
Product	enamelin precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF125373.1 and BC117308.1. This sequence is a reference standard in the RefSeqGene project. On Jun 7, 2008 this sequence version replaced NM_031889.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF125373.1, BC117308.1 [ECO:0000332] ##Evidence-Data-END##

ATGTTGGTGC TTCGGTGCAG GCTTGGAACC TCTTTTCCTA AACTAGATAA CTTGGTACCA 
AAAGGCAAAA TGAAGATTCT CCTGGTCTTT CTAGGGCTTC TTGGTAATTC TGTTGCTATG 
CCAATGCACA TGCCCCGAAT GCCTGGATTT AGCAGTAAAA GTGAGGAGAT GATGCGGTAT 
AATCAATTCA ACTTTATGAA CGGCCCACAT ATGGCACACC TGGGGCCCTT CTTTGGAAAC 
GGTCTCCCTC AGCAATTTCC ACAGTACCAG ATGCCCATGT GGCCTCAGCC ACCACCCAAC 
ACATGGCATC CACGGAAATC CTCAGCACCC AAACGTCATA ACAAGACTGA TCAGACCCAA 
GAAACCCAGA AACCCAACCA GACTCAGTCA AAAAAGCCAC CACAAAAGCG GCCTTTGAAG 
CAGCCATCAC ATAATCAACC TCAGCCCGAA GAGGAAGCTC AACCCCCTCA GGCATTCCCA 
CCATTTGGAA ATGGGCTATT CCCCTATCAA CAACCACCAT GGCAAATTCC ACAGAGGTTA 
CCACCACCAG GTTATGGACG CCCACCAATC AGCAATGAAG AAGGGGGGAA TCCTTACTTT 
GGATATTTTG GATATCATGG CTTTGGGGGT CGCCCTCCTT ATTATTCAGA AGAAATGTTT 
GAACAAGATT TTGAAAAACC CAAAGAAGAA GATCCTCCTA AAGCAGAAAG TCCAGGCACA 
GAACCCACAG CTAATTCAAC AGTCACTGAG ACGAATTCTA CCCAACCAAA TCCTAAAGGG 
AGTCAGGGAG GAAATGACAC CAGCCCCACA GGAAACAGTA CCCCAGGACT AAACACTGGG 
AACAACCCTC CAGCTCAAAA TGGGATTGGC CCACTCCCTG CAGTCAACGC TTCAGGCCAG 
GGAGGGCCAG GAAGTCAAAT CCCATGGAGA CCAAGTCAGC CAAATATTCG TGAAAATCAT 
CCATATCCTA ATATAAGAAA TTTTCCTTCA GGAAGACAGT GGTATTTCAC TGGTACTGTC 
ATGGGGCACA GACAGAATAG GCCTTTTTAC AGAAATCAAC AAGTTCAAAG GGGTCCTCGG 
TGGAACTTCT TTGCTTGGGA ACGTAAACAA GTAGCTCGTC CAGGAAATCC AGTTTATCAC 
AAAGCTTACC CTCCTACTTC AAGAGGCAAT TATCCCAATT ATGCAGGAAA TCCAGCAAAT 
CTCAGAAGAA AGCCTCAGGG GCCAAATAAA CACCCTGTAG GAACTACTGT TGCCCCACTG 
GGTCCCAAAC CTGGCCCTGT TGTTCGCAAT GAAAAAATCC AAAATCCAAA GGAGAAGCCC 
CTGGGTCCAA AAGAACAAAT AATAGTTCCT ACAAAGAATC CAACCAGCCC CTGGAGAAAC 
TCTCAACAGT ATGAAGTTAA TAAATCAAAT TATAAACTGC CTCACTCTGA GGGTTATATG 
CCAGTCCCAA ATTTTAATTC TGTTGATCAA CATGAAAACT CCTATTACCC AAGAGGAGAT 
TCCAGAAAAG TCCCAAATTC TGATGGACAA ACCCAAAGCC AGAATTTGCC CAAAGGGATT 
GTTTTAGGGT CAAGAAGGAT GCCATATGAA TCAGAAACTA ATCAGTCAGA ATTAAAGCAC 
AGCTCATATC AGCCTGCTGT ATACCCTGAG GAAATCCCTT CTCCTGCAAA AGAACATTTT 
CCTGCTGGAA GAAATACTTG GGACCACCAA GAAATCTCTC CACCTTTTAA GGAAGATCCA 
GGGAGGCAAG AAGAACATTT ACCCCATCCT TCCCATGGTT CTAGAGGAAG TGTTTTCTAC 
CCTGAATATA ACCCATATGA TCCCAGGGAA AACTCACCAT ACCTTAGAGG CAATACATGG 
GATGAGAGAG ATGATTCTCC CAATACTATG GGGCAAAAAG AAAGTCCACT CTACCCCATA 
AATACCCCAG ACCAGAAGGA GATAGTCCCT TATAATGAAG AGGACCCAGT TGATCCAACT 
GGAGATGAAG TCTTTCCTGG ACAAAATAGA TGGGGTGAAG AGTTGAGCTT CAAAGGAGGC 
CCAACAGTTA GGCACTATGA AGGTGAACAA TATACCTCAA ATCAGCCAAA GGAATATCTT 
CCCTATTCTT TAGATAATCC ATCAAAACCA AGGGAGGATT TTTATTACAG TGAATTTTAC 
CCATGGAGCC CGGATGAGAA TTTTCCATCA TATAATACAG CTTCTACTAT GCCACCACCT 
ATAGAGAGCA GGGGCTACTA CGTTAATAAT GCCGCTGGAC CAGAAGAAAG CACTCTATTT 
CCTTCACGGA ATTCCTGGGA CCACAGGATA CAAGCCCAAG GGCAGAGAGA AAGAAGGCCG 
TATTTTAACA GAAATATCTG GGATCAGGCA ACACATTTAC AAAAAGCCCC AGCTAGGCCA 
CCAGACCAGA AAGGTAACCA GCCCTATTAC AGTAACACCC CAGCTGGGCT TCAGAAAAAT 
CCAATATGGC ATGAAGGTGA GAATTTGAAC TATGGCATGC AAATAACTAG GATGAATTCT 
CCAGAGAGAG AACATTCATC TTTCCCTAAC TTCATCCCAC CAAGTTACCC ATCAGGTCAA 
AAAGAAGCAC ATTTATTTCA CCTAAGCCAG AGAGGCTCTT GCTGTGCTGG TAGCTCCACA 
GGGCCCAAGG ACAATCCACT AGCTCTACAA GACTACACTC CATCCTATGG TCTTGCACCT 
GGGGAGAACC AAGACACCAG TCCTCTGTAT ACAGACGGTA GTCATACCAA GCAGACAAGA 
GATATCATCT CCCCAACAAG CATCCTACCA GGCCAAAGAA ACAGCTCAGA GAAGAGGGAA 
AGCCAAAACC CTTTTAGAGA TGATGTGTCC ACGCTGAGGA GGAACACACC ATGTTCTATA 
AAGAATCAAC TGGGCCAAAA GGAAATTATG CCCTTTCCTG AAGCCAGTTC CCTTCAATCA 
AAGAATACAC CTTGTCTCAA AAATGATCTT GGAGGAGATG GGAACAACAT TCTGGAACAA 
GTTTTTGAAG ACAACCAGCT CAATGAAAGA ACTGTTGACC TTACTCCTGA GCAGCTTGTT 
ATTGGTACAC CTGATGAAGG CTCCAATCCA GAAGGCATCC AAAGTCAAGT CCAAGAAAAT 
GAGAGTGAGA GGCAACAGCA AAGACCATCT AACATTCTGC ATTTGCCATG CTTTGGCTCC 
AAATTAGCAA AGCATCACTC TTCCACCACC GGAACTCCAT CTAGCGATGG AAGGCAAAGC 
CCATTTGATG GGGATTCAAT TACGCCTACT GAAAATCCTA ACACATTGGT TGAGTTAGCT 
ACTGAGGAAC AATTTAAGAG TATAAATGTA GACCCACTTG ATGCAGATGA ACACAGTCCA 
TTTGAATTCC TTCAAAGAGG GACCAATGTA CAGGACCAGG TACAAGACTG CTTACTACTT 
CAGGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_114095.2
CDS	282..3710
Translation	MLVLRCRLGTSFPKLDNLVPKGKMKILLVFLGLLGNSVAMPMHMPRMPGFSSKSEEMMRYNQFNFMNGPHMAHLGPFFGNGLPQQFPQYQMPMWPQPPPNTWHPRKSSAPKRHNKTDQTQETQKPNQTQSKKPPQKRPLKQPSHNQPQPEEEAQPPQAFPPFGNGLFPYQQPPWQIPQRLPPPGYGRPPISNEEGGNPYFGYFGYHGFGGRPPYYSEEMFEQDFEKPKEEDPPKAESPGTEPTANSTVTETNSTQPNPKGSQGGNDTSPTGNSTPGLNTGNNPPAQNGIGPLPAVNASGQGGPGSQIPWRPSQPNIRENHPYPNIRNFPSGRQWYFTGTVMGHRQNRPFYRNQQVQRGPRWNFFAWERKQVARPGNPVYHKAYPPTSRGNYPNYAGNPANLRRKPQGPNKHPVGTTVAPLGPKPGPVVRNEKIQNPKEKPLGPKEQIIVPTKNPTSPWRNSQQYEVNKSNYKLPHSEGYMPVPNFNSVDQHENSYYPRGDSRKVPNSDGQTQSQNLPKGIVLGSRRMPYESETNQSELKHSSYQPAVYPEEIPSPAKEHFPAGRNTWDHQEISPPFKEDPGRQEEHLPHPSHGSRGSVFYPEYNPYDPRENSPYLRGNTWDERDDSPNTMGQKESPLYPINTPDQKEIVPYNEEDPVDPTGDEVFPGQNRWGEELSFKGGPTVRHYEGEQYTSNQPKEYLPYSLDNPSKPREDFYYSEFYPWSPDENFPSYNTASTMPPPIESRGYYVNNAAGPEESTLFPSRNSWDHRIQAQGQRERRPYFNRNIWDQATHLQKAPARPPDQKGNQPYYSNTPAGLQKNPIWHEGENLNYGMQITRMNSPEREHSSFPNFIPPSYPSGQKEAHLFHLSQRGSCCAGSSTGPKDNPLALQDYTPSYGLAPGENQDTSPLYTDGSHTKQTRDIISPTSILPGQRNSSEKRESQNPFRDDVSTLRRNTPCSIKNQLGQKEIMPFPEASSLQSKNTPCLKNDLGGDGNNILEQVFEDNQLNERTVDLTPEQLVIGTPDEGSNPEGIQSQVQENESERQQQRPSNILHLPCFGSKLAKHHSSTTGTPSSDGRQSPFDGDSITPTENPNTLVELATEEQFKSINVDPLDADEHSPFEFLQRGTNVQDQVQDCLLLQA

Target ORF information:

RefSeq Version	NM_031889.2
Organism	Homo sapiens(human)
Definition	Homo sapiens enamelin (ENAM), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031889.2

ORF Insert Sequence:

ATGTTGGTGC TTCGGTGCAG GCTTGGAACC TCTTTTCCTA AACTAGATAA CTTGGTACCA 
AAAGGCAAAA TGAAGATTCT CCTGGTCTTT CTAGGGCTTC TTGGTAATTC TGTTGCTATG 
CCAATGCACA TGCCCCGAAT GCCTGGATTT AGCAGTAAAA GTGAGGAGAT GATGCGGTAT 
AATCAATTCA ACTTTATGAA CGGCCCACAT ATGGCACACC TGGGGCCCTT CTTTGGAAAC 
GGTCTCCCTC AGCAATTTCC ACAGTACCAG ATGCCCATGT GGCCTCAGCC ACCACCCAAC 
ACATGGCATC CACGGAAATC CTCAGCACCC AAACGTCATA ACAAGACTGA TCAGACCCAA 
GAAACCCAGA AACCCAACCA GACTCAGTCA AAAAAGCCAC CACAAAAGCG GCCTTTGAAG 
CAGCCATCAC ATAATCAACC TCAGCCCGAA GAGGAAGCTC AACCCCCTCA GGCATTCCCA 
CCATTTGGAA ATGGGCTATT CCCCTATCAA CAACCACCAT GGCAAATTCC ACAGAGGTTA 
CCACCACCAG GTTATGGACG CCCACCAATC AGCAATGAAG AAGGGGGGAA TCCTTACTTT 
GGATATTTTG GATATCATGG CTTTGGGGGT CGCCCTCCTT ATTATTCAGA AGAAATGTTT 
GAACAAGATT TTGAAAAACC CAAAGAAGAA GATCCTCCTA AAGCAGAAAG TCCAGGCACA 
GAACCCACAG CTAATTCAAC AGTCACTGAG ACGAATTCTA CCCAACCAAA TCCTAAAGGG 
AGTCAGGGAG GAAATGACAC CAGCCCCACA GGAAACAGTA CCCCAGGACT AAACACTGGG 
AACAACCCTC CAGCTCAAAA TGGGATTGGC CCACTCCCTG CAGTCAACGC TTCAGGCCAG 
GGAGGGCCAG GAAGTCAAAT CCCATGGAGA CCAAGTCAGC CAAATATTCG TGAAAATCAT 
CCATATCCTA ATATAAGAAA TTTTCCTTCA GGAAGACAGT GGTATTTCAC TGGTACTGTC 
ATGGGGCACA GACAGAATAG GCCTTTTTAC AGAAATCAAC AAGTTCAAAG GGGTCCTCGG 
TGGAACTTCT TTGCTTGGGA ACGTAAACAA GTAGCTCGTC CAGGAAATCC AGTTTATCAC 
AAAGCTTACC CTCCTACTTC AAGAGGCAAT TATCCCAATT ATGCAGGAAA TCCAGCAAAT 
CTCAGAAGAA AGCCTCAGGG GCCAAATAAA CACCCTGTAG GAACTACTGT TGCCCCACTG 
GGTCCCAAAC CTGGCCCTGT TGTTCGCAAT GAAAAAATCC AAAATCCAAA GGAGAAGCCC 
CTGGGTCCAA AAGAACAAAT AATAGTTCCT ACAAAGAATC CAACCAGCCC CTGGAGAAAC 
TCTCAACAGT ATGAAGTTAA TAAATCAAAT TATAAACTGC CTCACTCTGA GGGTTATATG 
CCAGTCCCAA ATTTTAATTC TGTTGATCAA CATGAAAACT CCTATTACCC AAGAGGAGAT 
TCCAGAAAAG TCCCAAATTC TGATGGACAA ACCCAAAGCC AGAATTTGCC CAAAGGGATT 
GTTTTAGGGT CAAGAAGGAT GCCATATGAA TCAGAAACTA ATCAGTCAGA ATTAAAGCAC 
AGCTCATATC AGCCTGCTGT ATACCCTGAG GAAATCCCTT CTCCTGCAAA AGAACATTTT 
CCTGCTGGAA GAAATACTTG GGACCACCAA GAAATCTCTC CACCTTTTAA GGAAGATCCA 
GGGAGGCAAG AAGAACATTT ACCCCATCCT TCCCATGGTT CTAGAGGAAG TGTTTTCTAC 
CCTGAATATA ACCCATATGA TCCCAGGGAA AACTCACCAT ACCTTAGAGG CAATACATGG 
GATGAGAGAG ATGATTCTCC CAATACTATG GGGCAAAAAG AAAGTCCACT CTACCCCATA 
AATACCCCAG ACCAGAAGGA GATAGTCCCT TATAATGAAG AGGACCCAGT TGATCCAACT 
GGAGATGAAG TCTTTCCTGG ACAAAATAGA TGGGGTGAAG AGTTGAGCTT CAAAGGAGGC 
CCAACAGTTA GGCACTATGA AGGTGAACAA TATACCTCAA ATCAGCCAAA GGAATATCTT 
CCCTATTCTT TAGATAATCC ATCAAAACCA AGGGAGGATT TTTATTACAG TGAATTTTAC 
CCATGGAGCC CGGATGAGAA TTTTCCATCA TATAATACAG CTTCTACTAT GCCACCACCT 
ATAGAGAGCA GGGGCTACTA CGTTAATAAT GCCGCTGGAC CAGAAGAAAG CACTCTATTT 
CCTTCACGGA ATTCCTGGGA CCACAGGATA CAAGCCCAAG GGCAGAGAGA AAGAAGGCCG 
TATTTTAACA GAAATATCTG GGATCAGGCA ACACATTTAC AAAAAGCCCC AGCTAGGCCA 
CCAGACCAGA AAGGTAACCA GCCCTATTAC AGTAACACCC CAGCTGGGCT TCAGAAAAAT 
CCAATATGGC ATGAAGGTGA GAATTTGAAC TATGGCATGC AAATAACTAG GATGAATTCT 
CCAGAGAGAG AACATTCATC TTTCCCTAAC TTCATCCCAC CAAGTTACCC ATCAGGTCAA 
AAAGAAGCAC ATTTATTTCA CCTAAGCCAG AGAGGCTCTT GCTGTGCTGG TAGCTCCACA 
GGGCCCAAGG ACAATCCACT AGCTCTACAA GACTACACTC CATCCTATGG TCTTGCACCT 
GGGGAGAACC AAGACACCAG TCCTCTGTAT ACAGACGGTA GTCATACCAA GCAGACAAGA 
GATATCATCT CCCCAACAAG CATCCTACCA GGCCAAAGAA ACAGCTCAGA GAAGAGGGAA 
AGCCAAAACC CTTTTAGAGA TGATGTGTCC ACGCTGAGGA GGAACACACC ATGTTCTATA 
AAGAATCAAC TGGGCCAAAA GGAAATTATG CCCTTTCCTG AAGCCAGTTC CCTTCAATCA 
AAGAATACAC CTTGTCTCAA AAATGATCTT GGAGGAGATG GGAACAACAT TCTGGAACAA 
GTTTTTGAAG ACAACCAGCT CAATGAAAGA ACTGTTGACC TTACTCCTGA GCAGCTTGTT 
ATTGGTACAC CTGATGAAGG CTCCAATCCA GAAGGCATCC AAAGTCAAGT CCAAGAAAAT 
GAGAGTGAGA GGCAACAGCA AAGACCATCT AACATTCTGC ATTTGCCATG CTTTGGCTCC 
AAATTAGCAA AGCATCACTC TTCCACCACC GGAACTCCAT CTAGCGATGG AAGGCAAAGC 
CCATTTGATG GGGATTCAAT TACGCCTACT GAAAATCCTA ACACATTGGT TGAGTTAGCT 
ACTGAGGAAC AATTTAAGAG TATAAATGTA GACCCACTTG ATGCAGATGA ACACAGTCCA 
TTTGAATTCC TTCAAAGAGG GACCAATGTA CAGGACCAGG TACAAGACTG CTTACTACTT 
CAGGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ENAM ( NM_031889.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_114095.2 Homo sapiens enamelin (ENAM), transcript variant 1, mRNA.

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CloneID	OHu18574
Clone ID Related Accession (Same CDS sequence)	XM_006714056.2 , NM_031889.2 , NM_031889.3 , XM_006714056.3 , XM_006714056.4
Accession Version	NM_031889.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3429bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-03
Organism	Homo sapiens(human)
Product	enamelin isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF125373.1, BC117308.1 and AC009570.13. This sequence is a reference standard in the RefSeqGene project. On Jan 15, 2019 this sequence version replaced NM_031889.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF125373.1, BC117308.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on manual assertion, conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTTGGTGC TTCGGTGCAG GCTTGGAACC TCTTTTCCTA AACTAGATAA CTTGGTACCA 
AAAGGCAAAA TGAAGATTCT CCTGGTCTTT CTAGGGCTTC TTGGTAATTC TGTTGCTATG 
CCAATGCACA TGCCCCGAAT GCCTGGATTT AGCAGTAAAA GTGAGGAGAT GATGCGGTAT 
AATCAATTCA ACTTTATGAA CGGCCCACAT ATGGCACACC TGGGGCCCTT CTTTGGAAAC 
GGTCTCCCTC AGCAATTTCC ACAGTACCAG ATGCCCATGT GGCCTCAGCC ACCACCCAAC 
ACATGGCATC CACGGAAATC CTCAGCACCC AAACGTCATA ACAAGACTGA TCAGACCCAA 
GAAACCCAGA AACCCAACCA GACTCAGTCA AAAAAGCCAC CACAAAAGCG GCCTTTGAAG 
CAGCCATCAC ATAATCAACC TCAGCCCGAA GAGGAAGCTC AACCCCCTCA GGCATTCCCA 
CCATTTGGAA ATGGGCTATT CCCCTATCAA CAACCACCAT GGCAAATTCC ACAGAGGTTA 
CCACCACCAG GTTATGGACG CCCACCAATC AGCAATGAAG AAGGGGGGAA TCCTTACTTT 
GGATATTTTG GATATCATGG CTTTGGGGGT CGCCCTCCTT ATTATTCAGA AGAAATGTTT 
GAACAAGATT TTGAAAAACC CAAAGAAGAA GATCCTCCTA AAGCAGAAAG TCCAGGCACA 
GAACCCACAG CTAATTCAAC AGTCACTGAG ACGAATTCTA CCCAACCAAA TCCTAAAGGG 
AGTCAGGGAG GAAATGACAC CAGCCCCACA GGAAACAGTA CCCCAGGACT AAACACTGGG 
AACAACCCTC CAGCTCAAAA TGGGATTGGC CCACTCCCTG CAGTCAACGC TTCAGGCCAG 
GGAGGGCCAG GAAGTCAAAT CCCATGGAGA CCAAGTCAGC CAAATATTCG TGAAAATCAT 
CCATATCCTA ATATAAGAAA TTTTCCTTCA GGAAGACAGT GGTATTTCAC TGGTACTGTC 
ATGGGGCACA GACAGAATAG GCCTTTTTAC AGAAATCAAC AAGTTCAAAG GGGTCCTCGG 
TGGAACTTCT TTGCTTGGGA ACGTAAACAA GTAGCTCGTC CAGGAAATCC AGTTTATCAC 
AAAGCTTACC CTCCTACTTC AAGAGGCAAT TATCCCAATT ATGCAGGAAA TCCAGCAAAT 
CTCAGAAGAA AGCCTCAGGG GCCAAATAAA CACCCTGTAG GAACTACTGT TGCCCCACTG 
GGTCCCAAAC CTGGCCCTGT TGTTCGCAAT GAAAAAATCC AAAATCCAAA GGAGAAGCCC 
CTGGGTCCAA AAGAACAAAT AATAGTTCCT ACAAAGAATC CAACCAGCCC CTGGAGAAAC 
TCTCAACAGT ATGAAGTTAA TAAATCAAAT TATAAACTGC CTCACTCTGA GGGTTATATG 
CCAGTCCCAA ATTTTAATTC TGTTGATCAA CATGAAAACT CCTATTACCC AAGAGGAGAT 
TCCAGAAAAG TCCCAAATTC TGATGGACAA ACCCAAAGCC AGAATTTGCC CAAAGGGATT 
GTTTTAGGGT CAAGAAGGAT GCCATATGAA TCAGAAACTA ATCAGTCAGA ATTAAAGCAC 
AGCTCATATC AGCCTGCTGT ATACCCTGAG GAAATCCCTT CTCCTGCAAA AGAACATTTT 
CCTGCTGGAA GAAATACTTG GGACCACCAA GAAATCTCTC CACCTTTTAA GGAAGATCCA 
GGGAGGCAAG AAGAACATTT ACCCCATCCT TCCCATGGTT CTAGAGGAAG TGTTTTCTAC 
CCTGAATATA ACCCATATGA TCCCAGGGAA AACTCACCAT ACCTTAGAGG CAATACATGG 
GATGAGAGAG ATGATTCTCC CAATACTATG GGGCAAAAAG AAAGTCCACT CTACCCCATA 
AATACCCCAG ACCAGAAGGA GATAGTCCCT TATAATGAAG AGGACCCAGT TGATCCAACT 
GGAGATGAAG TCTTTCCTGG ACAAAATAGA TGGGGTGAAG AGTTGAGCTT CAAAGGAGGC 
CCAACAGTTA GGCACTATGA AGGTGAACAA TATACCTCAA ATCAGCCAAA GGAATATCTT 
CCCTATTCTT TAGATAATCC ATCAAAACCA AGGGAGGATT TTTATTACAG TGAATTTTAC 
CCATGGAGCC CGGATGAGAA TTTTCCATCA TATAATACAG CTTCTACTAT GCCACCACCT 
ATAGAGAGCA GGGGCTACTA CGTTAATAAT GCCGCTGGAC CAGAAGAAAG CACTCTATTT 
CCTTCACGGA ATTCCTGGGA CCACAGGATA CAAGCCCAAG GGCAGAGAGA AAGAAGGCCG 
TATTTTAACA GAAATATCTG GGATCAGGCA ACACATTTAC AAAAAGCCCC AGCTAGGCCA 
CCAGACCAGA AAGGTAACCA GCCCTATTAC AGTAACACCC CAGCTGGGCT TCAGAAAAAT 
CCAATATGGC ATGAAGGTGA GAATTTGAAC TATGGCATGC AAATAACTAG GATGAATTCT 
CCAGAGAGAG AACATTCATC TTTCCCTAAC TTCATCCCAC CAAGTTACCC ATCAGGTCAA 
AAAGAAGCAC ATTTATTTCA CCTAAGCCAG AGAGGCTCTT GCTGTGCTGG TAGCTCCACA 
GGGCCCAAGG ACAATCCACT AGCTCTACAA GACTACACTC CATCCTATGG TCTTGCACCT 
GGGGAGAACC AAGACACCAG TCCTCTGTAT ACAGACGGTA GTCATACCAA GCAGACAAGA 
GATATCATCT CCCCAACAAG CATCCTACCA GGCCAAAGAA ACAGCTCAGA GAAGAGGGAA 
AGCCAAAACC CTTTTAGAGA TGATGTGTCC ACGCTGAGGA GGAACACACC ATGTTCTATA 
AAGAATCAAC TGGGCCAAAA GGAAATTATG CCCTTTCCTG AAGCCAGTTC CCTTCAATCA 
AAGAATACAC CTTGTCTCAA AAATGATCTT GGAGGAGATG GGAACAACAT TCTGGAACAA 
GTTTTTGAAG ACAACCAGCT CAATGAAAGA ACTGTTGACC TTACTCCTGA GCAGCTTGTT 
ATTGGTACAC CTGATGAAGG CTCCAATCCA GAAGGCATCC AAAGTCAAGT CCAAGAAAAT 
GAGAGTGAGA GGCAACAGCA AAGACCATCT AACATTCTGC ATTTGCCATG CTTTGGCTCC 
AAATTAGCAA AGCATCACTC TTCCACCACC GGAACTCCAT CTAGCGATGG AAGGCAAAGC 
CCATTTGATG GGGATTCAAT TACGCCTACT GAAAATCCTA ACACATTGGT TGAGTTAGCT 
ACTGAGGAAC AATTTAAGAG TATAAATGTA GACCCACTTG ATGCAGATGA ACACAGTCCA 
TTTGAATTCC TTCAAAGAGG GACCAATGTA CAGGACCAGG TACAAGACTG CTTACTACTT 
CAGGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_114095.2
CDS	282..3710
Translation	MLVLRCRLGTSFPKLDNLVPKGKMKILLVFLGLLGNSVAMPMHMPRMPGFSSKSEEMMRYNQFNFMNGPHMAHLGPFFGNGLPQQFPQYQMPMWPQPPPNTWHPRKSSAPKRHNKTDQTQETQKPNQTQSKKPPQKRPLKQPSHNQPQPEEEAQPPQAFPPFGNGLFPYQQPPWQIPQRLPPPGYGRPPISNEEGGNPYFGYFGYHGFGGRPPYYSEEMFEQDFEKPKEEDPPKAESPGTEPTANSTVTETNSTQPNPKGSQGGNDTSPTGNSTPGLNTGNNPPAQNGIGPLPAVNASGQGGPGSQIPWRPSQPNIRENHPYPNIRNFPSGRQWYFTGTVMGHRQNRPFYRNQQVQRGPRWNFFAWERKQVARPGNPVYHKAYPPTSRGNYPNYAGNPANLRRKPQGPNKHPVGTTVAPLGPKPGPVVRNEKIQNPKEKPLGPKEQIIVPTKNPTSPWRNSQQYEVNKSNYKLPHSEGYMPVPNFNSVDQHENSYYPRGDSRKVPNSDGQTQSQNLPKGIVLGSRRMPYESETNQSELKHSSYQPAVYPEEIPSPAKEHFPAGRNTWDHQEISPPFKEDPGRQEEHLPHPSHGSRGSVFYPEYNPYDPRENSPYLRGNTWDERDDSPNTMGQKESPLYPINTPDQKEIVPYNEEDPVDPTGDEVFPGQNRWGEELSFKGGPTVRHYEGEQYTSNQPKEYLPYSLDNPSKPREDFYYSEFYPWSPDENFPSYNTASTMPPPIESRGYYVNNAAGPEESTLFPSRNSWDHRIQAQGQRERRPYFNRNIWDQATHLQKAPARPPDQKGNQPYYSNTPAGLQKNPIWHEGENLNYGMQITRMNSPEREHSSFPNFIPPSYPSGQKEAHLFHLSQRGSCCAGSSTGPKDNPLALQDYTPSYGLAPGENQDTSPLYTDGSHTKQTRDIISPTSILPGQRNSSEKRESQNPFRDDVSTLRRNTPCSIKNQLGQKEIMPFPEASSLQSKNTPCLKNDLGGDGNNILEQVFEDNQLNERTVDLTPEQLVIGTPDEGSNPEGIQSQVQENESERQQQRPSNILHLPCFGSKLAKHHSSTTGTPSSDGRQSPFDGDSITPTENPNTLVELATEEQFKSINVDPLDADEHSPFEFLQRGTNVQDQVQDCLLLQA

Target ORF information:

RefSeq Version	NM_031889.3
Organism	Homo sapiens(human)
Definition	Homo sapiens enamelin (ENAM), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031889.3

ORF Insert Sequence:

ATGTTGGTGC TTCGGTGCAG GCTTGGAACC TCTTTTCCTA AACTAGATAA CTTGGTACCA 
AAAGGCAAAA TGAAGATTCT CCTGGTCTTT CTAGGGCTTC TTGGTAATTC TGTTGCTATG 
CCAATGCACA TGCCCCGAAT GCCTGGATTT AGCAGTAAAA GTGAGGAGAT GATGCGGTAT 
AATCAATTCA ACTTTATGAA CGGCCCACAT ATGGCACACC TGGGGCCCTT CTTTGGAAAC 
GGTCTCCCTC AGCAATTTCC ACAGTACCAG ATGCCCATGT GGCCTCAGCC ACCACCCAAC 
ACATGGCATC CACGGAAATC CTCAGCACCC AAACGTCATA ACAAGACTGA TCAGACCCAA 
GAAACCCAGA AACCCAACCA GACTCAGTCA AAAAAGCCAC CACAAAAGCG GCCTTTGAAG 
CAGCCATCAC ATAATCAACC TCAGCCCGAA GAGGAAGCTC AACCCCCTCA GGCATTCCCA 
CCATTTGGAA ATGGGCTATT CCCCTATCAA CAACCACCAT GGCAAATTCC ACAGAGGTTA 
CCACCACCAG GTTATGGACG CCCACCAATC AGCAATGAAG AAGGGGGGAA TCCTTACTTT 
GGATATTTTG GATATCATGG CTTTGGGGGT CGCCCTCCTT ATTATTCAGA AGAAATGTTT 
GAACAAGATT TTGAAAAACC CAAAGAAGAA GATCCTCCTA AAGCAGAAAG TCCAGGCACA 
GAACCCACAG CTAATTCAAC AGTCACTGAG ACGAATTCTA CCCAACCAAA TCCTAAAGGG 
AGTCAGGGAG GAAATGACAC CAGCCCCACA GGAAACAGTA CCCCAGGACT AAACACTGGG 
AACAACCCTC CAGCTCAAAA TGGGATTGGC CCACTCCCTG CAGTCAACGC TTCAGGCCAG 
GGAGGGCCAG GAAGTCAAAT CCCATGGAGA CCAAGTCAGC CAAATATTCG TGAAAATCAT 
CCATATCCTA ATATAAGAAA TTTTCCTTCA GGAAGACAGT GGTATTTCAC TGGTACTGTC 
ATGGGGCACA GACAGAATAG GCCTTTTTAC AGAAATCAAC AAGTTCAAAG GGGTCCTCGG 
TGGAACTTCT TTGCTTGGGA ACGTAAACAA GTAGCTCGTC CAGGAAATCC AGTTTATCAC 
AAAGCTTACC CTCCTACTTC AAGAGGCAAT TATCCCAATT ATGCAGGAAA TCCAGCAAAT 
CTCAGAAGAA AGCCTCAGGG GCCAAATAAA CACCCTGTAG GAACTACTGT TGCCCCACTG 
GGTCCCAAAC CTGGCCCTGT TGTTCGCAAT GAAAAAATCC AAAATCCAAA GGAGAAGCCC 
CTGGGTCCAA AAGAACAAAT AATAGTTCCT ACAAAGAATC CAACCAGCCC CTGGAGAAAC 
TCTCAACAGT ATGAAGTTAA TAAATCAAAT TATAAACTGC CTCACTCTGA GGGTTATATG 
CCAGTCCCAA ATTTTAATTC TGTTGATCAA CATGAAAACT CCTATTACCC AAGAGGAGAT 
TCCAGAAAAG TCCCAAATTC TGATGGACAA ACCCAAAGCC AGAATTTGCC CAAAGGGATT 
GTTTTAGGGT CAAGAAGGAT GCCATATGAA TCAGAAACTA ATCAGTCAGA ATTAAAGCAC 
AGCTCATATC AGCCTGCTGT ATACCCTGAG GAAATCCCTT CTCCTGCAAA AGAACATTTT 
CCTGCTGGAA GAAATACTTG GGACCACCAA GAAATCTCTC CACCTTTTAA GGAAGATCCA 
GGGAGGCAAG AAGAACATTT ACCCCATCCT TCCCATGGTT CTAGAGGAAG TGTTTTCTAC 
CCTGAATATA ACCCATATGA TCCCAGGGAA AACTCACCAT ACCTTAGAGG CAATACATGG 
GATGAGAGAG ATGATTCTCC CAATACTATG GGGCAAAAAG AAAGTCCACT CTACCCCATA 
AATACCCCAG ACCAGAAGGA GATAGTCCCT TATAATGAAG AGGACCCAGT TGATCCAACT 
GGAGATGAAG TCTTTCCTGG ACAAAATAGA TGGGGTGAAG AGTTGAGCTT CAAAGGAGGC 
CCAACAGTTA GGCACTATGA AGGTGAACAA TATACCTCAA ATCAGCCAAA GGAATATCTT 
CCCTATTCTT TAGATAATCC ATCAAAACCA AGGGAGGATT TTTATTACAG TGAATTTTAC 
CCATGGAGCC CGGATGAGAA TTTTCCATCA TATAATACAG CTTCTACTAT GCCACCACCT 
ATAGAGAGCA GGGGCTACTA CGTTAATAAT GCCGCTGGAC CAGAAGAAAG CACTCTATTT 
CCTTCACGGA ATTCCTGGGA CCACAGGATA CAAGCCCAAG GGCAGAGAGA AAGAAGGCCG 
TATTTTAACA GAAATATCTG GGATCAGGCA ACACATTTAC AAAAAGCCCC AGCTAGGCCA 
CCAGACCAGA AAGGTAACCA GCCCTATTAC AGTAACACCC CAGCTGGGCT TCAGAAAAAT 
CCAATATGGC ATGAAGGTGA GAATTTGAAC TATGGCATGC AAATAACTAG GATGAATTCT 
CCAGAGAGAG AACATTCATC TTTCCCTAAC TTCATCCCAC CAAGTTACCC ATCAGGTCAA 
AAAGAAGCAC ATTTATTTCA CCTAAGCCAG AGAGGCTCTT GCTGTGCTGG TAGCTCCACA 
GGGCCCAAGG ACAATCCACT AGCTCTACAA GACTACACTC CATCCTATGG TCTTGCACCT 
GGGGAGAACC AAGACACCAG TCCTCTGTAT ACAGACGGTA GTCATACCAA GCAGACAAGA 
GATATCATCT CCCCAACAAG CATCCTACCA GGCCAAAGAA ACAGCTCAGA GAAGAGGGAA 
AGCCAAAACC CTTTTAGAGA TGATGTGTCC ACGCTGAGGA GGAACACACC ATGTTCTATA 
AAGAATCAAC TGGGCCAAAA GGAAATTATG CCCTTTCCTG AAGCCAGTTC CCTTCAATCA 
AAGAATACAC CTTGTCTCAA AAATGATCTT GGAGGAGATG GGAACAACAT TCTGGAACAA 
GTTTTTGAAG ACAACCAGCT CAATGAAAGA ACTGTTGACC TTACTCCTGA GCAGCTTGTT 
ATTGGTACAC CTGATGAAGG CTCCAATCCA GAAGGCATCC AAAGTCAAGT CCAAGAAAAT 
GAGAGTGAGA GGCAACAGCA AAGACCATCT AACATTCTGC ATTTGCCATG CTTTGGCTCC 
AAATTAGCAA AGCATCACTC TTCCACCACC GGAACTCCAT CTAGCGATGG AAGGCAAAGC 
CCATTTGATG GGGATTCAAT TACGCCTACT GAAAATCCTA ACACATTGGT TGAGTTAGCT 
ACTGAGGAAC AATTTAAGAG TATAAATGTA GACCCACTTG ATGCAGATGA ACACAGTCCA 
TTTGAATTCC TTCAAAGAGG GACCAATGTA CAGGACCAGG TACAAGACTG CTTACTACTT 
CAGGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ENAM ( XM_006714056.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_006714119.1 Homo sapiens enamelin (ENAM), transcript variant X1, mRNA.

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CloneID	OHu18574
Clone ID Related Accession (Same CDS sequence)	XM_006714056.2 , NM_031889.2 , NM_031889.3 , XM_006714056.3 , XM_006714056.4
Accession Version	XM_006714056.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3429bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	enamelin isoform X1
Comment

ATGTTGGTGC TTCGGTGCAG GCTTGGAACC TCTTTTCCTA AACTAGATAA CTTGGTACCA 
AAAGGCAAAA TGAAGATTCT CCTGGTCTTT CTAGGGCTTC TTGGTAATTC TGTTGCTATG 
CCAATGCACA TGCCCCGAAT GCCTGGATTT AGCAGTAAAA GTGAGGAGAT GATGCGGTAT 
AATCAATTCA ACTTTATGAA CGGCCCACAT ATGGCACACC TGGGGCCCTT CTTTGGAAAC 
GGTCTCCCTC AGCAATTTCC ACAGTACCAG ATGCCCATGT GGCCTCAGCC ACCACCCAAC 
ACATGGCATC CACGGAAATC CTCAGCACCC AAACGTCATA ACAAGACTGA TCAGACCCAA 
GAAACCCAGA AACCCAACCA GACTCAGTCA AAAAAGCCAC CACAAAAGCG GCCTTTGAAG 
CAGCCATCAC ATAATCAACC TCAGCCCGAA GAGGAAGCTC AACCCCCTCA GGCATTCCCA 
CCATTTGGAA ATGGGCTATT CCCCTATCAA CAACCACCAT GGCAAATTCC ACAGAGGTTA 
CCACCACCAG GTTATGGACG CCCACCAATC AGCAATGAAG AAGGGGGGAA TCCTTACTTT 
GGATATTTTG GATATCATGG CTTTGGGGGT CGCCCTCCTT ATTATTCAGA AGAAATGTTT 
GAACAAGATT TTGAAAAACC CAAAGAAGAA GATCCTCCTA AAGCAGAAAG TCCAGGCACA 
GAACCCACAG CTAATTCAAC AGTCACTGAG ACGAATTCTA CCCAACCAAA TCCTAAAGGG 
AGTCAGGGAG GAAATGACAC CAGCCCCACA GGAAACAGTA CCCCAGGACT AAACACTGGG 
AACAACCCTC CAGCTCAAAA TGGGATTGGC CCACTCCCTG CAGTCAACGC TTCAGGCCAG 
GGAGGGCCAG GAAGTCAAAT CCCATGGAGA CCAAGTCAGC CAAATATTCG TGAAAATCAT 
CCATATCCTA ATATAAGAAA TTTTCCTTCA GGAAGACAGT GGTATTTCAC TGGTACTGTC 
ATGGGGCACA GACAGAATAG GCCTTTTTAC AGAAATCAAC AAGTTCAAAG GGGTCCTCGG 
TGGAACTTCT TTGCTTGGGA ACGTAAACAA GTAGCTCGTC CAGGAAATCC AGTTTATCAC 
AAAGCTTACC CTCCTACTTC AAGAGGCAAT TATCCCAATT ATGCAGGAAA TCCAGCAAAT 
CTCAGAAGAA AGCCTCAGGG GCCAAATAAA CACCCTGTAG GAACTACTGT TGCCCCACTG 
GGTCCCAAAC CTGGCCCTGT TGTTCGCAAT GAAAAAATCC AAAATCCAAA GGAGAAGCCC 
CTGGGTCCAA AAGAACAAAT AATAGTTCCT ACAAAGAATC CAACCAGCCC CTGGAGAAAC 
TCTCAACAGT ATGAAGTTAA TAAATCAAAT TATAAACTGC CTCACTCTGA GGGTTATATG 
CCAGTCCCAA ATTTTAATTC TGTTGATCAA CATGAAAACT CCTATTACCC AAGAGGAGAT 
TCCAGAAAAG TCCCAAATTC TGATGGACAA ACCCAAAGCC AGAATTTGCC CAAAGGGATT 
GTTTTAGGGT CAAGAAGGAT GCCATATGAA TCAGAAACTA ATCAGTCAGA ATTAAAGCAC 
AGCTCATATC AGCCTGCTGT ATACCCTGAG GAAATCCCTT CTCCTGCAAA AGAACATTTT 
CCTGCTGGAA GAAATACTTG GGACCACCAA GAAATCTCTC CACCTTTTAA GGAAGATCCA 
GGGAGGCAAG AAGAACATTT ACCCCATCCT TCCCATGGTT CTAGAGGAAG TGTTTTCTAC 
CCTGAATATA ACCCATATGA TCCCAGGGAA AACTCACCAT ACCTTAGAGG CAATACATGG 
GATGAGAGAG ATGATTCTCC CAATACTATG GGGCAAAAAG AAAGTCCACT CTACCCCATA 
AATACCCCAG ACCAGAAGGA GATAGTCCCT TATAATGAAG AGGACCCAGT TGATCCAACT 
GGAGATGAAG TCTTTCCTGG ACAAAATAGA TGGGGTGAAG AGTTGAGCTT CAAAGGAGGC 
CCAACAGTTA GGCACTATGA AGGTGAACAA TATACCTCAA ATCAGCCAAA GGAATATCTT 
CCCTATTCTT TAGATAATCC ATCAAAACCA AGGGAGGATT TTTATTACAG TGAATTTTAC 
CCATGGAGCC CGGATGAGAA TTTTCCATCA TATAATACAG CTTCTACTAT GCCACCACCT 
ATAGAGAGCA GGGGCTACTA CGTTAATAAT GCCGCTGGAC CAGAAGAAAG CACTCTATTT 
CCTTCACGGA ATTCCTGGGA CCACAGGATA CAAGCCCAAG GGCAGAGAGA AAGAAGGCCG 
TATTTTAACA GAAATATCTG GGATCAGGCA ACACATTTAC AAAAAGCCCC AGCTAGGCCA 
CCAGACCAGA AAGGTAACCA GCCCTATTAC AGTAACACCC CAGCTGGGCT TCAGAAAAAT 
CCAATATGGC ATGAAGGTGA GAATTTGAAC TATGGCATGC AAATAACTAG GATGAATTCT 
CCAGAGAGAG AACATTCATC TTTCCCTAAC TTCATCCCAC CAAGTTACCC ATCAGGTCAA 
AAAGAAGCAC ATTTATTTCA CCTAAGCCAG AGAGGCTCTT GCTGTGCTGG TAGCTCCACA 
GGGCCCAAGG ACAATCCACT AGCTCTACAA GACTACACTC CATCCTATGG TCTTGCACCT 
GGGGAGAACC AAGACACCAG TCCTCTGTAT ACAGACGGTA GTCATACCAA GCAGACAAGA 
GATATCATCT CCCCAACAAG CATCCTACCA GGCCAAAGAA ACAGCTCAGA GAAGAGGGAA 
AGCCAAAACC CTTTTAGAGA TGATGTGTCC ACGCTGAGGA GGAACACACC ATGTTCTATA 
AAGAATCAAC TGGGCCAAAA GGAAATTATG CCCTTTCCTG AAGCCAGTTC CCTTCAATCA 
AAGAATACAC CTTGTCTCAA AAATGATCTT GGAGGAGATG GGAACAACAT TCTGGAACAA 
GTTTTTGAAG ACAACCAGCT CAATGAAAGA ACTGTTGACC TTACTCCTGA GCAGCTTGTT 
ATTGGTACAC CTGATGAAGG CTCCAATCCA GAAGGCATCC AAAGTCAAGT CCAAGAAAAT 
GAGAGTGAGA GGCAACAGCA AAGACCATCT AACATTCTGC ATTTGCCATG CTTTGGCTCC 
AAATTAGCAA AGCATCACTC TTCCACCACC GGAACTCCAT CTAGCGATGG AAGGCAAAGC 
CCATTTGATG GGGATTCAAT TACGCCTACT GAAAATCCTA ACACATTGGT TGAGTTAGCT 
ACTGAGGAAC AATTTAAGAG TATAAATGTA GACCCACTTG ATGCAGATGA ACACAGTCCA 
TTTGAATTCC TTCAAAGAGG GACCAATGTA CAGGACCAGG TACAAGACTG CTTACTACTT 
CAGGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_006714119.1

Translation

MLVLRCRLGTSFPKLDNLVPKGKMKILLVFLGLLGNSVAMPMHMPRMPGFSSKSEEMMRYNQFNFMNGPHMAHLGPFFGNGLPQQFPQYQMPMWPQPPPNTWHPRKSSAPKRHNKTDQTQETQKPNQTQSKKPPQKRPLKQPSHNQPQPEEEAQPPQAFPPFGNGLFPYQQPPWQIPQRLPPPGYGRPPISNEEGGNPYFGYFGYHGFGGRPPYYSEEMFEQDFEKPKEEDPPKAESPGTEPTANSTVTETNSTQPNPKGSQGGNDTSPTGNSTPGLNTGNNPPAQNGIGPLPAVNASGQGGPGSQIPWRPSQPNIRENHPYPNIRNFPSGRQWYFTGTVMGHRQNRPFYRNQQVQRGPRWNFFAWERKQVARPGNPVYHKAYPPTSRGNYPNYAGNPANLRRKPQGPNKHPVGTTVAPLGPKPGPVVRNEKIQNPKEKPLGPKEQIIVPTKNPTSPWRNSQQYEVNKSNYKLPHSEGYMPVPNFNSVDQHENSYYPRGDSRKVPNSDGQTQSQNLPKGIVLGSRRMPYESETNQSELKHSSYQPAVYPEEIPSPAKEHFPAGRNTWDHQEISPPFKEDPGRQEEHLPHPSHGSRGSVFYPEYNPYDPRENSPYLRGNTWDERDDSPNTMGQKESPLYPINTPDQKEIVPYNEEDPVDPTGDEVFPGQNRWGEELSFKGGPTVRHYEGEQYTSNQPKEYLPYSLDNPSKPREDFYYSEFYPWSPDENFPSYNTASTMPPPIESRGYYVNNAAGPEESTLFPSRNSWDHRIQAQGQRERRPYFNRNIWDQATHLQKAPARPPDQKGNQPYYSNTPAGLQKNPIWHEGENLNYGMQITRMNSPEREHSSFPNFIPPSYPSGQKEAHLFHLSQRGSCCAGSSTGPKDNPLALQDYTPSYGLAPGENQDTSPLYTDGSHTKQTRDIISPTSILPGQRNSSEKRESQNPFRDDVSTLRRNTPCSIKNQLGQKEIMPFPEASSLQSKNTPCLKNDLGGDGNNILEQVFEDNQLNERTVDLTPEQLVIGTPDEGSNPEGIQSQVQENESERQQQRPSNILHLPCFGSKLAKHHSSTTGTPSSDGRQSPFDGDSITPTENPNTLVELATEEQFKSINVDPLDADEHSPFEFLQRGTNVQDQVQDCLLLQA

Target ORF information:

RefSeq Version	XM_006714056.2
Organism	Homo sapiens(Human)
Definition	Homo sapiens enamelin (ENAM), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006714056.2

ORF Insert Sequence:

ATGTTGGTGC TTCGGTGCAG GCTTGGAACC TCTTTTCCTA AACTAGATAA CTTGGTACCA 
AAAGGCAAAA TGAAGATTCT CCTGGTCTTT CTAGGGCTTC TTGGTAATTC TGTTGCTATG 
CCAATGCACA TGCCCCGAAT GCCTGGATTT AGCAGTAAAA GTGAGGAGAT GATGCGGTAT 
AATCAATTCA ACTTTATGAA CGGCCCACAT ATGGCACACC TGGGGCCCTT CTTTGGAAAC 
GGTCTCCCTC AGCAATTTCC ACAGTACCAG ATGCCCATGT GGCCTCAGCC ACCACCCAAC 
ACATGGCATC CACGGAAATC CTCAGCACCC AAACGTCATA ACAAGACTGA TCAGACCCAA 
GAAACCCAGA AACCCAACCA GACTCAGTCA AAAAAGCCAC CACAAAAGCG GCCTTTGAAG 
CAGCCATCAC ATAATCAACC TCAGCCCGAA GAGGAAGCTC AACCCCCTCA GGCATTCCCA 
CCATTTGGAA ATGGGCTATT CCCCTATCAA CAACCACCAT GGCAAATTCC ACAGAGGTTA 
CCACCACCAG GTTATGGACG CCCACCAATC AGCAATGAAG AAGGGGGGAA TCCTTACTTT 
GGATATTTTG GATATCATGG CTTTGGGGGT CGCCCTCCTT ATTATTCAGA AGAAATGTTT 
GAACAAGATT TTGAAAAACC CAAAGAAGAA GATCCTCCTA AAGCAGAAAG TCCAGGCACA 
GAACCCACAG CTAATTCAAC AGTCACTGAG ACGAATTCTA CCCAACCAAA TCCTAAAGGG 
AGTCAGGGAG GAAATGACAC CAGCCCCACA GGAAACAGTA CCCCAGGACT AAACACTGGG 
AACAACCCTC CAGCTCAAAA TGGGATTGGC CCACTCCCTG CAGTCAACGC TTCAGGCCAG 
GGAGGGCCAG GAAGTCAAAT CCCATGGAGA CCAAGTCAGC CAAATATTCG TGAAAATCAT 
CCATATCCTA ATATAAGAAA TTTTCCTTCA GGAAGACAGT GGTATTTCAC TGGTACTGTC 
ATGGGGCACA GACAGAATAG GCCTTTTTAC AGAAATCAAC AAGTTCAAAG GGGTCCTCGG 
TGGAACTTCT TTGCTTGGGA ACGTAAACAA GTAGCTCGTC CAGGAAATCC AGTTTATCAC 
AAAGCTTACC CTCCTACTTC AAGAGGCAAT TATCCCAATT ATGCAGGAAA TCCAGCAAAT 
CTCAGAAGAA AGCCTCAGGG GCCAAATAAA CACCCTGTAG GAACTACTGT TGCCCCACTG 
GGTCCCAAAC CTGGCCCTGT TGTTCGCAAT GAAAAAATCC AAAATCCAAA GGAGAAGCCC 
CTGGGTCCAA AAGAACAAAT AATAGTTCCT ACAAAGAATC CAACCAGCCC CTGGAGAAAC 
TCTCAACAGT ATGAAGTTAA TAAATCAAAT TATAAACTGC CTCACTCTGA GGGTTATATG 
CCAGTCCCAA ATTTTAATTC TGTTGATCAA CATGAAAACT CCTATTACCC AAGAGGAGAT 
TCCAGAAAAG TCCCAAATTC TGATGGACAA ACCCAAAGCC AGAATTTGCC CAAAGGGATT 
GTTTTAGGGT CAAGAAGGAT GCCATATGAA TCAGAAACTA ATCAGTCAGA ATTAAAGCAC 
AGCTCATATC AGCCTGCTGT ATACCCTGAG GAAATCCCTT CTCCTGCAAA AGAACATTTT 
CCTGCTGGAA GAAATACTTG GGACCACCAA GAAATCTCTC CACCTTTTAA GGAAGATCCA 
GGGAGGCAAG AAGAACATTT ACCCCATCCT TCCCATGGTT CTAGAGGAAG TGTTTTCTAC 
CCTGAATATA ACCCATATGA TCCCAGGGAA AACTCACCAT ACCTTAGAGG CAATACATGG 
GATGAGAGAG ATGATTCTCC CAATACTATG GGGCAAAAAG AAAGTCCACT CTACCCCATA 
AATACCCCAG ACCAGAAGGA GATAGTCCCT TATAATGAAG AGGACCCAGT TGATCCAACT 
GGAGATGAAG TCTTTCCTGG ACAAAATAGA TGGGGTGAAG AGTTGAGCTT CAAAGGAGGC 
CCAACAGTTA GGCACTATGA AGGTGAACAA TATACCTCAA ATCAGCCAAA GGAATATCTT 
CCCTATTCTT TAGATAATCC ATCAAAACCA AGGGAGGATT TTTATTACAG TGAATTTTAC 
CCATGGAGCC CGGATGAGAA TTTTCCATCA TATAATACAG CTTCTACTAT GCCACCACCT 
ATAGAGAGCA GGGGCTACTA CGTTAATAAT GCCGCTGGAC CAGAAGAAAG CACTCTATTT 
CCTTCACGGA ATTCCTGGGA CCACAGGATA CAAGCCCAAG GGCAGAGAGA AAGAAGGCCG 
TATTTTAACA GAAATATCTG GGATCAGGCA ACACATTTAC AAAAAGCCCC AGCTAGGCCA 
CCAGACCAGA AAGGTAACCA GCCCTATTAC AGTAACACCC CAGCTGGGCT TCAGAAAAAT 
CCAATATGGC ATGAAGGTGA GAATTTGAAC TATGGCATGC AAATAACTAG GATGAATTCT 
CCAGAGAGAG AACATTCATC TTTCCCTAAC TTCATCCCAC CAAGTTACCC ATCAGGTCAA 
AAAGAAGCAC ATTTATTTCA CCTAAGCCAG AGAGGCTCTT GCTGTGCTGG TAGCTCCACA 
GGGCCCAAGG ACAATCCACT AGCTCTACAA GACTACACTC CATCCTATGG TCTTGCACCT 
GGGGAGAACC AAGACACCAG TCCTCTGTAT ACAGACGGTA GTCATACCAA GCAGACAAGA 
GATATCATCT CCCCAACAAG CATCCTACCA GGCCAAAGAA ACAGCTCAGA GAAGAGGGAA 
AGCCAAAACC CTTTTAGAGA TGATGTGTCC ACGCTGAGGA GGAACACACC ATGTTCTATA 
AAGAATCAAC TGGGCCAAAA GGAAATTATG CCCTTTCCTG AAGCCAGTTC CCTTCAATCA 
AAGAATACAC CTTGTCTCAA AAATGATCTT GGAGGAGATG GGAACAACAT TCTGGAACAA 
GTTTTTGAAG ACAACCAGCT CAATGAAAGA ACTGTTGACC TTACTCCTGA GCAGCTTGTT 
ATTGGTACAC CTGATGAAGG CTCCAATCCA GAAGGCATCC AAAGTCAAGT CCAAGAAAAT 
GAGAGTGAGA GGCAACAGCA AAGACCATCT AACATTCTGC ATTTGCCATG CTTTGGCTCC 
AAATTAGCAA AGCATCACTC TTCCACCACC GGAACTCCAT CTAGCGATGG AAGGCAAAGC 
CCATTTGATG GGGATTCAAT TACGCCTACT GAAAATCCTA ACACATTGGT TGAGTTAGCT 
ACTGAGGAAC AATTTAAGAG TATAAATGTA GACCCACTTG ATGCAGATGA ACACAGTCCA 
TTTGAATTCC TTCAAAGAGG GACCAATGTA CAGGACCAGG TACAAGACTG CTTACTACTT 
CAGGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ENAM ( XM_006714056.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006714119.1 Homo sapiens enamelin (ENAM), transcript variant X1, mRNA.

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CloneID	OHu18574
Clone ID Related Accession (Same CDS sequence)	XM_006714056.2 , NM_031889.2 , NM_031889.3 , XM_006714056.3 , XM_006714056.4
Accession Version	XM_006714056.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3429bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	enamelin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006714056.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGTGC TTCGGTGCAG GCTTGGAACC TCTTTTCCTA AACTAGATAA CTTGGTACCA 
AAAGGCAAAA TGAAGATTCT CCTGGTCTTT CTAGGGCTTC TTGGTAATTC TGTTGCTATG 
CCAATGCACA TGCCCCGAAT GCCTGGATTT AGCAGTAAAA GTGAGGAGAT GATGCGGTAT 
AATCAATTCA ACTTTATGAA CGGCCCACAT ATGGCACACC TGGGGCCCTT CTTTGGAAAC 
GGTCTCCCTC AGCAATTTCC ACAGTACCAG ATGCCCATGT GGCCTCAGCC ACCACCCAAC 
ACATGGCATC CACGGAAATC CTCAGCACCC AAACGTCATA ACAAGACTGA TCAGACCCAA 
GAAACCCAGA AACCCAACCA GACTCAGTCA AAAAAGCCAC CACAAAAGCG GCCTTTGAAG 
CAGCCATCAC ATAATCAACC TCAGCCCGAA GAGGAAGCTC AACCCCCTCA GGCATTCCCA 
CCATTTGGAA ATGGGCTATT CCCCTATCAA CAACCACCAT GGCAAATTCC ACAGAGGTTA 
CCACCACCAG GTTATGGACG CCCACCAATC AGCAATGAAG AAGGGGGGAA TCCTTACTTT 
GGATATTTTG GATATCATGG CTTTGGGGGT CGCCCTCCTT ATTATTCAGA AGAAATGTTT 
GAACAAGATT TTGAAAAACC CAAAGAAGAA GATCCTCCTA AAGCAGAAAG TCCAGGCACA 
GAACCCACAG CTAATTCAAC AGTCACTGAG ACGAATTCTA CCCAACCAAA TCCTAAAGGG 
AGTCAGGGAG GAAATGACAC CAGCCCCACA GGAAACAGTA CCCCAGGACT AAACACTGGG 
AACAACCCTC CAGCTCAAAA TGGGATTGGC CCACTCCCTG CAGTCAACGC TTCAGGCCAG 
GGAGGGCCAG GAAGTCAAAT CCCATGGAGA CCAAGTCAGC CAAATATTCG TGAAAATCAT 
CCATATCCTA ATATAAGAAA TTTTCCTTCA GGAAGACAGT GGTATTTCAC TGGTACTGTC 
ATGGGGCACA GACAGAATAG GCCTTTTTAC AGAAATCAAC AAGTTCAAAG GGGTCCTCGG 
TGGAACTTCT TTGCTTGGGA ACGTAAACAA GTAGCTCGTC CAGGAAATCC AGTTTATCAC 
AAAGCTTACC CTCCTACTTC AAGAGGCAAT TATCCCAATT ATGCAGGAAA TCCAGCAAAT 
CTCAGAAGAA AGCCTCAGGG GCCAAATAAA CACCCTGTAG GAACTACTGT TGCCCCACTG 
GGTCCCAAAC CTGGCCCTGT TGTTCGCAAT GAAAAAATCC AAAATCCAAA GGAGAAGCCC 
CTGGGTCCAA AAGAACAAAT AATAGTTCCT ACAAAGAATC CAACCAGCCC CTGGAGAAAC 
TCTCAACAGT ATGAAGTTAA TAAATCAAAT TATAAACTGC CTCACTCTGA GGGTTATATG 
CCAGTCCCAA ATTTTAATTC TGTTGATCAA CATGAAAACT CCTATTACCC AAGAGGAGAT 
TCCAGAAAAG TCCCAAATTC TGATGGACAA ACCCAAAGCC AGAATTTGCC CAAAGGGATT 
GTTTTAGGGT CAAGAAGGAT GCCATATGAA TCAGAAACTA ATCAGTCAGA ATTAAAGCAC 
AGCTCATATC AGCCTGCTGT ATACCCTGAG GAAATCCCTT CTCCTGCAAA AGAACATTTT 
CCTGCTGGAA GAAATACTTG GGACCACCAA GAAATCTCTC CACCTTTTAA GGAAGATCCA 
GGGAGGCAAG AAGAACATTT ACCCCATCCT TCCCATGGTT CTAGAGGAAG TGTTTTCTAC 
CCTGAATATA ACCCATATGA TCCCAGGGAA AACTCACCAT ACCTTAGAGG CAATACATGG 
GATGAGAGAG ATGATTCTCC CAATACTATG GGGCAAAAAG AAAGTCCACT CTACCCCATA 
AATACCCCAG ACCAGAAGGA GATAGTCCCT TATAATGAAG AGGACCCAGT TGATCCAACT 
GGAGATGAAG TCTTTCCTGG ACAAAATAGA TGGGGTGAAG AGTTGAGCTT CAAAGGAGGC 
CCAACAGTTA GGCACTATGA AGGTGAACAA TATACCTCAA ATCAGCCAAA GGAATATCTT 
CCCTATTCTT TAGATAATCC ATCAAAACCA AGGGAGGATT TTTATTACAG TGAATTTTAC 
CCATGGAGCC CGGATGAGAA TTTTCCATCA TATAATACAG CTTCTACTAT GCCACCACCT 
ATAGAGAGCA GGGGCTACTA CGTTAATAAT GCCGCTGGAC CAGAAGAAAG CACTCTATTT 
CCTTCACGGA ATTCCTGGGA CCACAGGATA CAAGCCCAAG GGCAGAGAGA AAGAAGGCCG 
TATTTTAACA GAAATATCTG GGATCAGGCA ACACATTTAC AAAAAGCCCC AGCTAGGCCA 
CCAGACCAGA AAGGTAACCA GCCCTATTAC AGTAACACCC CAGCTGGGCT TCAGAAAAAT 
CCAATATGGC ATGAAGGTGA GAATTTGAAC TATGGCATGC AAATAACTAG GATGAATTCT 
CCAGAGAGAG AACATTCATC TTTCCCTAAC TTCATCCCAC CAAGTTACCC ATCAGGTCAA 
AAAGAAGCAC ATTTATTTCA CCTAAGCCAG AGAGGCTCTT GCTGTGCTGG TAGCTCCACA 
GGGCCCAAGG ACAATCCACT AGCTCTACAA GACTACACTC CATCCTATGG TCTTGCACCT 
GGGGAGAACC AAGACACCAG TCCTCTGTAT ACAGACGGTA GTCATACCAA GCAGACAAGA 
GATATCATCT CCCCAACAAG CATCCTACCA GGCCAAAGAA ACAGCTCAGA GAAGAGGGAA 
AGCCAAAACC CTTTTAGAGA TGATGTGTCC ACGCTGAGGA GGAACACACC ATGTTCTATA 
AAGAATCAAC TGGGCCAAAA GGAAATTATG CCCTTTCCTG AAGCCAGTTC CCTTCAATCA 
AAGAATACAC CTTGTCTCAA AAATGATCTT GGAGGAGATG GGAACAACAT TCTGGAACAA 
GTTTTTGAAG ACAACCAGCT CAATGAAAGA ACTGTTGACC TTACTCCTGA GCAGCTTGTT 
ATTGGTACAC CTGATGAAGG CTCCAATCCA GAAGGCATCC AAAGTCAAGT CCAAGAAAAT 
GAGAGTGAGA GGCAACAGCA AAGACCATCT AACATTCTGC ATTTGCCATG CTTTGGCTCC 
AAATTAGCAA AGCATCACTC TTCCACCACC GGAACTCCAT CTAGCGATGG AAGGCAAAGC 
CCATTTGATG GGGATTCAAT TACGCCTACT GAAAATCCTA ACACATTGGT TGAGTTAGCT 
ACTGAGGAAC AATTTAAGAG TATAAATGTA GACCCACTTG ATGCAGATGA ACACAGTCCA 
TTTGAATTCC TTCAAAGAGG GACCAATGTA CAGGACCAGG TACAAGACTG CTTACTACTT 
CAGGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006714119.1
CDS	2031..5459
Translation	MLVLRCRLGTSFPKLDNLVPKGKMKILLVFLGLLGNSVAMPMHMPRMPGFSSKSEEMMRYNQFNFMNGPHMAHLGPFFGNGLPQQFPQYQMPMWPQPPPNTWHPRKSSAPKRHNKTDQTQETQKPNQTQSKKPPQKRPLKQPSHNQPQPEEEAQPPQAFPPFGNGLFPYQQPPWQIPQRLPPPGYGRPPISNEEGGNPYFGYFGYHGFGGRPPYYSEEMFEQDFEKPKEEDPPKAESPGTEPTANSTVTETNSTQPNPKGSQGGNDTSPTGNSTPGLNTGNNPPAQNGIGPLPAVNASGQGGPGSQIPWRPSQPNIRENHPYPNIRNFPSGRQWYFTGTVMGHRQNRPFYRNQQVQRGPRWNFFAWERKQVARPGNPVYHKAYPPTSRGNYPNYAGNPANLRRKPQGPNKHPVGTTVAPLGPKPGPVVRNEKIQNPKEKPLGPKEQIIVPTKNPTSPWRNSQQYEVNKSNYKLPHSEGYMPVPNFNSVDQHENSYYPRGDSRKVPNSDGQTQSQNLPKGIVLGSRRMPYESETNQSELKHSSYQPAVYPEEIPSPAKEHFPAGRNTWDHQEISPPFKEDPGRQEEHLPHPSHGSRGSVFYPEYNPYDPRENSPYLRGNTWDERDDSPNTMGQKESPLYPINTPDQKEIVPYNEEDPVDPTGDEVFPGQNRWGEELSFKGGPTVRHYEGEQYTSNQPKEYLPYSLDNPSKPREDFYYSEFYPWSPDENFPSYNTASTMPPPIESRGYYVNNAAGPEESTLFPSRNSWDHRIQAQGQRERRPYFNRNIWDQATHLQKAPARPPDQKGNQPYYSNTPAGLQKNPIWHEGENLNYGMQITRMNSPEREHSSFPNFIPPSYPSGQKEAHLFHLSQRGSCCAGSSTGPKDNPLALQDYTPSYGLAPGENQDTSPLYTDGSHTKQTRDIISPTSILPGQRNSSEKRESQNPFRDDVSTLRRNTPCSIKNQLGQKEIMPFPEASSLQSKNTPCLKNDLGGDGNNILEQVFEDNQLNERTVDLTPEQLVIGTPDEGSNPEGIQSQVQENESERQQQRPSNILHLPCFGSKLAKHHSSTTGTPSSDGRQSPFDGDSITPTENPNTLVELATEEQFKSINVDPLDADEHSPFEFLQRGTNVQDQVQDCLLLQA

Target ORF information:

RefSeq Version	XM_006714056.3
Organism	Homo sapiens(human)
Definition	Homo sapiens enamelin (ENAM), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006714056.3

ORF Insert Sequence:

ATGTTGGTGC TTCGGTGCAG GCTTGGAACC TCTTTTCCTA AACTAGATAA CTTGGTACCA 
AAAGGCAAAA TGAAGATTCT CCTGGTCTTT CTAGGGCTTC TTGGTAATTC TGTTGCTATG 
CCAATGCACA TGCCCCGAAT GCCTGGATTT AGCAGTAAAA GTGAGGAGAT GATGCGGTAT 
AATCAATTCA ACTTTATGAA CGGCCCACAT ATGGCACACC TGGGGCCCTT CTTTGGAAAC 
GGTCTCCCTC AGCAATTTCC ACAGTACCAG ATGCCCATGT GGCCTCAGCC ACCACCCAAC 
ACATGGCATC CACGGAAATC CTCAGCACCC AAACGTCATA ACAAGACTGA TCAGACCCAA 
GAAACCCAGA AACCCAACCA GACTCAGTCA AAAAAGCCAC CACAAAAGCG GCCTTTGAAG 
CAGCCATCAC ATAATCAACC TCAGCCCGAA GAGGAAGCTC AACCCCCTCA GGCATTCCCA 
CCATTTGGAA ATGGGCTATT CCCCTATCAA CAACCACCAT GGCAAATTCC ACAGAGGTTA 
CCACCACCAG GTTATGGACG CCCACCAATC AGCAATGAAG AAGGGGGGAA TCCTTACTTT 
GGATATTTTG GATATCATGG CTTTGGGGGT CGCCCTCCTT ATTATTCAGA AGAAATGTTT 
GAACAAGATT TTGAAAAACC CAAAGAAGAA GATCCTCCTA AAGCAGAAAG TCCAGGCACA 
GAACCCACAG CTAATTCAAC AGTCACTGAG ACGAATTCTA CCCAACCAAA TCCTAAAGGG 
AGTCAGGGAG GAAATGACAC CAGCCCCACA GGAAACAGTA CCCCAGGACT AAACACTGGG 
AACAACCCTC CAGCTCAAAA TGGGATTGGC CCACTCCCTG CAGTCAACGC TTCAGGCCAG 
GGAGGGCCAG GAAGTCAAAT CCCATGGAGA CCAAGTCAGC CAAATATTCG TGAAAATCAT 
CCATATCCTA ATATAAGAAA TTTTCCTTCA GGAAGACAGT GGTATTTCAC TGGTACTGTC 
ATGGGGCACA GACAGAATAG GCCTTTTTAC AGAAATCAAC AAGTTCAAAG GGGTCCTCGG 
TGGAACTTCT TTGCTTGGGA ACGTAAACAA GTAGCTCGTC CAGGAAATCC AGTTTATCAC 
AAAGCTTACC CTCCTACTTC AAGAGGCAAT TATCCCAATT ATGCAGGAAA TCCAGCAAAT 
CTCAGAAGAA AGCCTCAGGG GCCAAATAAA CACCCTGTAG GAACTACTGT TGCCCCACTG 
GGTCCCAAAC CTGGCCCTGT TGTTCGCAAT GAAAAAATCC AAAATCCAAA GGAGAAGCCC 
CTGGGTCCAA AAGAACAAAT AATAGTTCCT ACAAAGAATC CAACCAGCCC CTGGAGAAAC 
TCTCAACAGT ATGAAGTTAA TAAATCAAAT TATAAACTGC CTCACTCTGA GGGTTATATG 
CCAGTCCCAA ATTTTAATTC TGTTGATCAA CATGAAAACT CCTATTACCC AAGAGGAGAT 
TCCAGAAAAG TCCCAAATTC TGATGGACAA ACCCAAAGCC AGAATTTGCC CAAAGGGATT 
GTTTTAGGGT CAAGAAGGAT GCCATATGAA TCAGAAACTA ATCAGTCAGA ATTAAAGCAC 
AGCTCATATC AGCCTGCTGT ATACCCTGAG GAAATCCCTT CTCCTGCAAA AGAACATTTT 
CCTGCTGGAA GAAATACTTG GGACCACCAA GAAATCTCTC CACCTTTTAA GGAAGATCCA 
GGGAGGCAAG AAGAACATTT ACCCCATCCT TCCCATGGTT CTAGAGGAAG TGTTTTCTAC 
CCTGAATATA ACCCATATGA TCCCAGGGAA AACTCACCAT ACCTTAGAGG CAATACATGG 
GATGAGAGAG ATGATTCTCC CAATACTATG GGGCAAAAAG AAAGTCCACT CTACCCCATA 
AATACCCCAG ACCAGAAGGA GATAGTCCCT TATAATGAAG AGGACCCAGT TGATCCAACT 
GGAGATGAAG TCTTTCCTGG ACAAAATAGA TGGGGTGAAG AGTTGAGCTT CAAAGGAGGC 
CCAACAGTTA GGCACTATGA AGGTGAACAA TATACCTCAA ATCAGCCAAA GGAATATCTT 
CCCTATTCTT TAGATAATCC ATCAAAACCA AGGGAGGATT TTTATTACAG TGAATTTTAC 
CCATGGAGCC CGGATGAGAA TTTTCCATCA TATAATACAG CTTCTACTAT GCCACCACCT 
ATAGAGAGCA GGGGCTACTA CGTTAATAAT GCCGCTGGAC CAGAAGAAAG CACTCTATTT 
CCTTCACGGA ATTCCTGGGA CCACAGGATA CAAGCCCAAG GGCAGAGAGA AAGAAGGCCG 
TATTTTAACA GAAATATCTG GGATCAGGCA ACACATTTAC AAAAAGCCCC AGCTAGGCCA 
CCAGACCAGA AAGGTAACCA GCCCTATTAC AGTAACACCC CAGCTGGGCT TCAGAAAAAT 
CCAATATGGC ATGAAGGTGA GAATTTGAAC TATGGCATGC AAATAACTAG GATGAATTCT 
CCAGAGAGAG AACATTCATC TTTCCCTAAC TTCATCCCAC CAAGTTACCC ATCAGGTCAA 
AAAGAAGCAC ATTTATTTCA CCTAAGCCAG AGAGGCTCTT GCTGTGCTGG TAGCTCCACA 
GGGCCCAAGG ACAATCCACT AGCTCTACAA GACTACACTC CATCCTATGG TCTTGCACCT 
GGGGAGAACC AAGACACCAG TCCTCTGTAT ACAGACGGTA GTCATACCAA GCAGACAAGA 
GATATCATCT CCCCAACAAG CATCCTACCA GGCCAAAGAA ACAGCTCAGA GAAGAGGGAA 
AGCCAAAACC CTTTTAGAGA TGATGTGTCC ACGCTGAGGA GGAACACACC ATGTTCTATA 
AAGAATCAAC TGGGCCAAAA GGAAATTATG CCCTTTCCTG AAGCCAGTTC CCTTCAATCA 
AAGAATACAC CTTGTCTCAA AAATGATCTT GGAGGAGATG GGAACAACAT TCTGGAACAA 
GTTTTTGAAG ACAACCAGCT CAATGAAAGA ACTGTTGACC TTACTCCTGA GCAGCTTGTT 
ATTGGTACAC CTGATGAAGG CTCCAATCCA GAAGGCATCC AAAGTCAAGT CCAAGAAAAT 
GAGAGTGAGA GGCAACAGCA AAGACCATCT AACATTCTGC ATTTGCCATG CTTTGGCTCC 
AAATTAGCAA AGCATCACTC TTCCACCACC GGAACTCCAT CTAGCGATGG AAGGCAAAGC 
CCATTTGATG GGGATTCAAT TACGCCTACT GAAAATCCTA ACACATTGGT TGAGTTAGCT 
ACTGAGGAAC AATTTAAGAG TATAAATGTA GACCCACTTG ATGCAGATGA ACACAGTCCA 
TTTGAATTCC TTCAAAGAGG GACCAATGTA CAGGACCAGG TACAAGACTG CTTACTACTT 
CAGGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ENAM ( XM_006714056.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_006714119.1 Homo sapiens enamelin (ENAM), transcript variant X1, mRNA.

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CloneID	OHu18574
Clone ID Related Accession (Same CDS sequence)	XM_006714056.2 , NM_031889.2 , NM_031889.3 , XM_006714056.3 , XM_006714056.4
Accession Version	XM_006714056.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3429bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	enamelin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_006714056.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGTGC TTCGGTGCAG GCTTGGAACC TCTTTTCCTA AACTAGATAA CTTGGTACCA 
AAAGGCAAAA TGAAGATTCT CCTGGTCTTT CTAGGGCTTC TTGGTAATTC TGTTGCTATG 
CCAATGCACA TGCCCCGAAT GCCTGGATTT AGCAGTAAAA GTGAGGAGAT GATGCGGTAT 
AATCAATTCA ACTTTATGAA CGGCCCACAT ATGGCACACC TGGGGCCCTT CTTTGGAAAC 
GGTCTCCCTC AGCAATTTCC ACAGTACCAG ATGCCCATGT GGCCTCAGCC ACCACCCAAC 
ACATGGCATC CACGGAAATC CTCAGCACCC AAACGTCATA ACAAGACTGA TCAGACCCAA 
GAAACCCAGA AACCCAACCA GACTCAGTCA AAAAAGCCAC CACAAAAGCG GCCTTTGAAG 
CAGCCATCAC ATAATCAACC TCAGCCCGAA GAGGAAGCTC AACCCCCTCA GGCATTCCCA 
CCATTTGGAA ATGGGCTATT CCCCTATCAA CAACCACCAT GGCAAATTCC ACAGAGGTTA 
CCACCACCAG GTTATGGACG CCCACCAATC AGCAATGAAG AAGGGGGGAA TCCTTACTTT 
GGATATTTTG GATATCATGG CTTTGGGGGT CGCCCTCCTT ATTATTCAGA AGAAATGTTT 
GAACAAGATT TTGAAAAACC CAAAGAAGAA GATCCTCCTA AAGCAGAAAG TCCAGGCACA 
GAACCCACAG CTAATTCAAC AGTCACTGAG ACGAATTCTA CCCAACCAAA TCCTAAAGGG 
AGTCAGGGAG GAAATGACAC CAGCCCCACA GGAAACAGTA CCCCAGGACT AAACACTGGG 
AACAACCCTC CAGCTCAAAA TGGGATTGGC CCACTCCCTG CAGTCAACGC TTCAGGCCAG 
GGAGGGCCAG GAAGTCAAAT CCCATGGAGA CCAAGTCAGC CAAATATTCG TGAAAATCAT 
CCATATCCTA ATATAAGAAA TTTTCCTTCA GGAAGACAGT GGTATTTCAC TGGTACTGTC 
ATGGGGCACA GACAGAATAG GCCTTTTTAC AGAAATCAAC AAGTTCAAAG GGGTCCTCGG 
TGGAACTTCT TTGCTTGGGA ACGTAAACAA GTAGCTCGTC CAGGAAATCC AGTTTATCAC 
AAAGCTTACC CTCCTACTTC AAGAGGCAAT TATCCCAATT ATGCAGGAAA TCCAGCAAAT 
CTCAGAAGAA AGCCTCAGGG GCCAAATAAA CACCCTGTAG GAACTACTGT TGCCCCACTG 
GGTCCCAAAC CTGGCCCTGT TGTTCGCAAT GAAAAAATCC AAAATCCAAA GGAGAAGCCC 
CTGGGTCCAA AAGAACAAAT AATAGTTCCT ACAAAGAATC CAACCAGCCC CTGGAGAAAC 
TCTCAACAGT ATGAAGTTAA TAAATCAAAT TATAAACTGC CTCACTCTGA GGGTTATATG 
CCAGTCCCAA ATTTTAATTC TGTTGATCAA CATGAAAACT CCTATTACCC AAGAGGAGAT 
TCCAGAAAAG TCCCAAATTC TGATGGACAA ACCCAAAGCC AGAATTTGCC CAAAGGGATT 
GTTTTAGGGT CAAGAAGGAT GCCATATGAA TCAGAAACTA ATCAGTCAGA ATTAAAGCAC 
AGCTCATATC AGCCTGCTGT ATACCCTGAG GAAATCCCTT CTCCTGCAAA AGAACATTTT 
CCTGCTGGAA GAAATACTTG GGACCACCAA GAAATCTCTC CACCTTTTAA GGAAGATCCA 
GGGAGGCAAG AAGAACATTT ACCCCATCCT TCCCATGGTT CTAGAGGAAG TGTTTTCTAC 
CCTGAATATA ACCCATATGA TCCCAGGGAA AACTCACCAT ACCTTAGAGG CAATACATGG 
GATGAGAGAG ATGATTCTCC CAATACTATG GGGCAAAAAG AAAGTCCACT CTACCCCATA 
AATACCCCAG ACCAGAAGGA GATAGTCCCT TATAATGAAG AGGACCCAGT TGATCCAACT 
GGAGATGAAG TCTTTCCTGG ACAAAATAGA TGGGGTGAAG AGTTGAGCTT CAAAGGAGGC 
CCAACAGTTA GGCACTATGA AGGTGAACAA TATACCTCAA ATCAGCCAAA GGAATATCTT 
CCCTATTCTT TAGATAATCC ATCAAAACCA AGGGAGGATT TTTATTACAG TGAATTTTAC 
CCATGGAGCC CGGATGAGAA TTTTCCATCA TATAATACAG CTTCTACTAT GCCACCACCT 
ATAGAGAGCA GGGGCTACTA CGTTAATAAT GCCGCTGGAC CAGAAGAAAG CACTCTATTT 
CCTTCACGGA ATTCCTGGGA CCACAGGATA CAAGCCCAAG GGCAGAGAGA AAGAAGGCCG 
TATTTTAACA GAAATATCTG GGATCAGGCA ACACATTTAC AAAAAGCCCC AGCTAGGCCA 
CCAGACCAGA AAGGTAACCA GCCCTATTAC AGTAACACCC CAGCTGGGCT TCAGAAAAAT 
CCAATATGGC ATGAAGGTGA GAATTTGAAC TATGGCATGC AAATAACTAG GATGAATTCT 
CCAGAGAGAG AACATTCATC TTTCCCTAAC TTCATCCCAC CAAGTTACCC ATCAGGTCAA 
AAAGAAGCAC ATTTATTTCA CCTAAGCCAG AGAGGCTCTT GCTGTGCTGG TAGCTCCACA 
GGGCCCAAGG ACAATCCACT AGCTCTACAA GACTACACTC CATCCTATGG TCTTGCACCT 
GGGGAGAACC AAGACACCAG TCCTCTGTAT ACAGACGGTA GTCATACCAA GCAGACAAGA 
GATATCATCT CCCCAACAAG CATCCTACCA GGCCAAAGAA ACAGCTCAGA GAAGAGGGAA 
AGCCAAAACC CTTTTAGAGA TGATGTGTCC ACGCTGAGGA GGAACACACC ATGTTCTATA 
AAGAATCAAC TGGGCCAAAA GGAAATTATG CCCTTTCCTG AAGCCAGTTC CCTTCAATCA 
AAGAATACAC CTTGTCTCAA AAATGATCTT GGAGGAGATG GGAACAACAT TCTGGAACAA 
GTTTTTGAAG ACAACCAGCT CAATGAAAGA ACTGTTGACC TTACTCCTGA GCAGCTTGTT 
ATTGGTACAC CTGATGAAGG CTCCAATCCA GAAGGCATCC AAAGTCAAGT CCAAGAAAAT 
GAGAGTGAGA GGCAACAGCA AAGACCATCT AACATTCTGC ATTTGCCATG CTTTGGCTCC 
AAATTAGCAA AGCATCACTC TTCCACCACC GGAACTCCAT CTAGCGATGG AAGGCAAAGC 
CCATTTGATG GGGATTCAAT TACGCCTACT GAAAATCCTA ACACATTGGT TGAGTTAGCT 
ACTGAGGAAC AATTTAAGAG TATAAATGTA GACCCACTTG ATGCAGATGA ACACAGTCCA 
TTTGAATTCC TTCAAAGAGG GACCAATGTA CAGGACCAGG TACAAGACTG CTTACTACTT 
CAGGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006714119.1
CDS	2031..5459
Translation	MLVLRCRLGTSFPKLDNLVPKGKMKILLVFLGLLGNSVAMPMHMPRMPGFSSKSEEMMRYNQFNFMNGPHMAHLGPFFGNGLPQQFPQYQMPMWPQPPPNTWHPRKSSAPKRHNKTDQTQETQKPNQTQSKKPPQKRPLKQPSHNQPQPEEEAQPPQAFPPFGNGLFPYQQPPWQIPQRLPPPGYGRPPISNEEGGNPYFGYFGYHGFGGRPPYYSEEMFEQDFEKPKEEDPPKAESPGTEPTANSTVTETNSTQPNPKGSQGGNDTSPTGNSTPGLNTGNNPPAQNGIGPLPAVNASGQGGPGSQIPWRPSQPNIRENHPYPNIRNFPSGRQWYFTGTVMGHRQNRPFYRNQQVQRGPRWNFFAWERKQVARPGNPVYHKAYPPTSRGNYPNYAGNPANLRRKPQGPNKHPVGTTVAPLGPKPGPVVRNEKIQNPKEKPLGPKEQIIVPTKNPTSPWRNSQQYEVNKSNYKLPHSEGYMPVPNFNSVDQHENSYYPRGDSRKVPNSDGQTQSQNLPKGIVLGSRRMPYESETNQSELKHSSYQPAVYPEEIPSPAKEHFPAGRNTWDHQEISPPFKEDPGRQEEHLPHPSHGSRGSVFYPEYNPYDPRENSPYLRGNTWDERDDSPNTMGQKESPLYPINTPDQKEIVPYNEEDPVDPTGDEVFPGQNRWGEELSFKGGPTVRHYEGEQYTSNQPKEYLPYSLDNPSKPREDFYYSEFYPWSPDENFPSYNTASTMPPPIESRGYYVNNAAGPEESTLFPSRNSWDHRIQAQGQRERRPYFNRNIWDQATHLQKAPARPPDQKGNQPYYSNTPAGLQKNPIWHEGENLNYGMQITRMNSPEREHSSFPNFIPPSYPSGQKEAHLFHLSQRGSCCAGSSTGPKDNPLALQDYTPSYGLAPGENQDTSPLYTDGSHTKQTRDIISPTSILPGQRNSSEKRESQNPFRDDVSTLRRNTPCSIKNQLGQKEIMPFPEASSLQSKNTPCLKNDLGGDGNNILEQVFEDNQLNERTVDLTPEQLVIGTPDEGSNPEGIQSQVQENESERQQQRPSNILHLPCFGSKLAKHHSSTTGTPSSDGRQSPFDGDSITPTENPNTLVELATEEQFKSINVDPLDADEHSPFEFLQRGTNVQDQVQDCLLLQA

Target ORF information:

RefSeq Version	XM_006714056.4
Organism	Homo sapiens(human)
Definition	Homo sapiens enamelin (ENAM), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006714056.4

ORF Insert Sequence:

ATGTTGGTGC TTCGGTGCAG GCTTGGAACC TCTTTTCCTA AACTAGATAA CTTGGTACCA 
AAAGGCAAAA TGAAGATTCT CCTGGTCTTT CTAGGGCTTC TTGGTAATTC TGTTGCTATG 
CCAATGCACA TGCCCCGAAT GCCTGGATTT AGCAGTAAAA GTGAGGAGAT GATGCGGTAT 
AATCAATTCA ACTTTATGAA CGGCCCACAT ATGGCACACC TGGGGCCCTT CTTTGGAAAC 
GGTCTCCCTC AGCAATTTCC ACAGTACCAG ATGCCCATGT GGCCTCAGCC ACCACCCAAC 
ACATGGCATC CACGGAAATC CTCAGCACCC AAACGTCATA ACAAGACTGA TCAGACCCAA 
GAAACCCAGA AACCCAACCA GACTCAGTCA AAAAAGCCAC CACAAAAGCG GCCTTTGAAG 
CAGCCATCAC ATAATCAACC TCAGCCCGAA GAGGAAGCTC AACCCCCTCA GGCATTCCCA 
CCATTTGGAA ATGGGCTATT CCCCTATCAA CAACCACCAT GGCAAATTCC ACAGAGGTTA 
CCACCACCAG GTTATGGACG CCCACCAATC AGCAATGAAG AAGGGGGGAA TCCTTACTTT 
GGATATTTTG GATATCATGG CTTTGGGGGT CGCCCTCCTT ATTATTCAGA AGAAATGTTT 
GAACAAGATT TTGAAAAACC CAAAGAAGAA GATCCTCCTA AAGCAGAAAG TCCAGGCACA 
GAACCCACAG CTAATTCAAC AGTCACTGAG ACGAATTCTA CCCAACCAAA TCCTAAAGGG 
AGTCAGGGAG GAAATGACAC CAGCCCCACA GGAAACAGTA CCCCAGGACT AAACACTGGG 
AACAACCCTC CAGCTCAAAA TGGGATTGGC CCACTCCCTG CAGTCAACGC TTCAGGCCAG 
GGAGGGCCAG GAAGTCAAAT CCCATGGAGA CCAAGTCAGC CAAATATTCG TGAAAATCAT 
CCATATCCTA ATATAAGAAA TTTTCCTTCA GGAAGACAGT GGTATTTCAC TGGTACTGTC 
ATGGGGCACA GACAGAATAG GCCTTTTTAC AGAAATCAAC AAGTTCAAAG GGGTCCTCGG 
TGGAACTTCT TTGCTTGGGA ACGTAAACAA GTAGCTCGTC CAGGAAATCC AGTTTATCAC 
AAAGCTTACC CTCCTACTTC AAGAGGCAAT TATCCCAATT ATGCAGGAAA TCCAGCAAAT 
CTCAGAAGAA AGCCTCAGGG GCCAAATAAA CACCCTGTAG GAACTACTGT TGCCCCACTG 
GGTCCCAAAC CTGGCCCTGT TGTTCGCAAT GAAAAAATCC AAAATCCAAA GGAGAAGCCC 
CTGGGTCCAA AAGAACAAAT AATAGTTCCT ACAAAGAATC CAACCAGCCC CTGGAGAAAC 
TCTCAACAGT ATGAAGTTAA TAAATCAAAT TATAAACTGC CTCACTCTGA GGGTTATATG 
CCAGTCCCAA ATTTTAATTC TGTTGATCAA CATGAAAACT CCTATTACCC AAGAGGAGAT 
TCCAGAAAAG TCCCAAATTC TGATGGACAA ACCCAAAGCC AGAATTTGCC CAAAGGGATT 
GTTTTAGGGT CAAGAAGGAT GCCATATGAA TCAGAAACTA ATCAGTCAGA ATTAAAGCAC 
AGCTCATATC AGCCTGCTGT ATACCCTGAG GAAATCCCTT CTCCTGCAAA AGAACATTTT 
CCTGCTGGAA GAAATACTTG GGACCACCAA GAAATCTCTC CACCTTTTAA GGAAGATCCA 
GGGAGGCAAG AAGAACATTT ACCCCATCCT TCCCATGGTT CTAGAGGAAG TGTTTTCTAC 
CCTGAATATA ACCCATATGA TCCCAGGGAA AACTCACCAT ACCTTAGAGG CAATACATGG 
GATGAGAGAG ATGATTCTCC CAATACTATG GGGCAAAAAG AAAGTCCACT CTACCCCATA 
AATACCCCAG ACCAGAAGGA GATAGTCCCT TATAATGAAG AGGACCCAGT TGATCCAACT 
GGAGATGAAG TCTTTCCTGG ACAAAATAGA TGGGGTGAAG AGTTGAGCTT CAAAGGAGGC 
CCAACAGTTA GGCACTATGA AGGTGAACAA TATACCTCAA ATCAGCCAAA GGAATATCTT 
CCCTATTCTT TAGATAATCC ATCAAAACCA AGGGAGGATT TTTATTACAG TGAATTTTAC 
CCATGGAGCC CGGATGAGAA TTTTCCATCA TATAATACAG CTTCTACTAT GCCACCACCT 
ATAGAGAGCA GGGGCTACTA CGTTAATAAT GCCGCTGGAC CAGAAGAAAG CACTCTATTT 
CCTTCACGGA ATTCCTGGGA CCACAGGATA CAAGCCCAAG GGCAGAGAGA AAGAAGGCCG 
TATTTTAACA GAAATATCTG GGATCAGGCA ACACATTTAC AAAAAGCCCC AGCTAGGCCA 
CCAGACCAGA AAGGTAACCA GCCCTATTAC AGTAACACCC CAGCTGGGCT TCAGAAAAAT 
CCAATATGGC ATGAAGGTGA GAATTTGAAC TATGGCATGC AAATAACTAG GATGAATTCT 
CCAGAGAGAG AACATTCATC TTTCCCTAAC TTCATCCCAC CAAGTTACCC ATCAGGTCAA 
AAAGAAGCAC ATTTATTTCA CCTAAGCCAG AGAGGCTCTT GCTGTGCTGG TAGCTCCACA 
GGGCCCAAGG ACAATCCACT AGCTCTACAA GACTACACTC CATCCTATGG TCTTGCACCT 
GGGGAGAACC AAGACACCAG TCCTCTGTAT ACAGACGGTA GTCATACCAA GCAGACAAGA 
GATATCATCT CCCCAACAAG CATCCTACCA GGCCAAAGAA ACAGCTCAGA GAAGAGGGAA 
AGCCAAAACC CTTTTAGAGA TGATGTGTCC ACGCTGAGGA GGAACACACC ATGTTCTATA 
AAGAATCAAC TGGGCCAAAA GGAAATTATG CCCTTTCCTG AAGCCAGTTC CCTTCAATCA 
AAGAATACAC CTTGTCTCAA AAATGATCTT GGAGGAGATG GGAACAACAT TCTGGAACAA 
GTTTTTGAAG ACAACCAGCT CAATGAAAGA ACTGTTGACC TTACTCCTGA GCAGCTTGTT 
ATTGGTACAC CTGATGAAGG CTCCAATCCA GAAGGCATCC AAAGTCAAGT CCAAGAAAAT 
GAGAGTGAGA GGCAACAGCA AAGACCATCT AACATTCTGC ATTTGCCATG CTTTGGCTCC 
AAATTAGCAA AGCATCACTC TTCCACCACC GGAACTCCAT CTAGCGATGG AAGGCAAAGC 
CCATTTGATG GGGATTCAAT TACGCCTACT GAAAATCCTA ACACATTGGT TGAGTTAGCT 
ACTGAGGAAC AATTTAAGAG TATAAATGTA GACCCACTTG ATGCAGATGA ACACAGTCCA 
TTTGAATTCC TTCAAAGAGG GACCAATGTA CAGGACCAGG TACAAGACTG CTTACTACTT 
CAGGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Lack of association between ENAM gene polymorphism and dental caries in primary and permanent teeth in Czech children.
Clinical oral investigations(2017 Nov)
Borilova Linhartova P,Deissova T,Musilova K,Zackova L,Kukletova M,Kukla L,Izakovicova Holla L

The association of Enamelin, Lactoferrin, and Tumour necrosis factor alpha gene polymorphisms with high caries susceptibility in Chinese children under 4 years old.
Archives of oral biology8075-81(2017 Aug)
Wang M,Qin M,Xia B

Neanderthal and Denisova tooth protein variants in present-day humans.
PloS one12(9)e0183802(2017)
Zanolli C,Hourset M,Esclassan R,Mollereau C

Human enamel thickness and ENAM polymorphism.
International journal of oral science8(2)93-7(2016 06)
Daubert DM,Kelley JL,Udod YG,Habor C,Kleist CG,Furman IK,Tikonov IN,Swanson WJ,Roberts FA

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ENAM cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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