TSFM cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	TSFM
Entrez Gene ID	10102
Full Name	Ts translation elongation factor, mitochondrial
Synonyms	EFTS,EFTSMT
General protein information	Preferred Names Ts translation elongation factor, mitochondrial Names elongation factor Ts, mitochondrial EF-Ts EF-TsMt mitochondrial elongation factor Ts
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 12 Map Location: 12q14.1
Summary	This gene encodes a mitochondrial translation elongation factor. The encoded protein is an enzyme that catalyzes the exchange of guanine nucleotides on the translation elongation factor Tu during the elongation step of mitchondrial protein translation. Mutations in this gene are associated with combined oxidative phosphorylation deficiency-3 syndrome. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Mar 2010].
Disorder MIM:	604723
Disorder Html:	Combined oxidative phosphorylation deficiency 3, 610505 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001172695.1	NP_001166166.1	elongation factor Ts, mitochondrial isoform 3 precursor
NM_001172696.2	NP_001166167.1	elongation factor Ts, mitochondrial isoform 1 precursor
NM_001172697.1	NP_001166168.1	elongation factor Ts, mitochondrial isoform 4 precursor
NM_001172695.2	NP_001166166.1	elongation factor Ts, mitochondrial isoform 3 precursor
NM_001172696.1	NP_001166167.1	elongation factor Ts, mitochondrial isoform 1 precursor
NM_005726.5	NP_005717.3	elongation factor Ts, mitochondrial isoform 2 precursor
NM_005726.6	NP_005717.3	elongation factor Ts, mitochondrial isoform 2 precursor

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	TSFM	XP_001167221.1
	TSFM	XP_001116504.2
Rattus norvegicus (Norway rat)	Tsfm	NP_001263405.1
Drosophila melanogaster (fruit fly)	CG6412	NP_609847.1
Caenorhabditis elegans (roundworm)	tsfm-1	NP_506079.1
Xenopus tropicalis (tropical clawed frog)	tsfm	XP_002933736.2
Mus musculus (house mouse)	Tsfm	NP_079813.1
Homo sapiens (human)	TSFM	NP_001166167.1
Bos taurus (cattle)	TSFM	NP_776629.1
Danio rerio (zebrafish)	tsfm	NP_001073504.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009234	XP_320011.4

Gene Ontology
Bibliography

Related articles in PubMed

Molecular-genetic characterization and rescue of a TSFM mutation causing childhood-onset ataxia and nonobstructive cardiomyopathy.
Emperador S, Bayona-Bafaluy MP, Fern?ndez-Marmiesse A, Pineda M, Felgueroso B, L?pez-Gallardo E, Artuch R, Roca I, Ruiz-Pesini E, Couce ML, Montoya J
European journal of human genetics : EJHG25(1)153-156(2016 01)

Widespread macromolecular interaction perturbations in human genetic disorders.
Sahni N, Yi S, Taipale M, Fuxman Bass JI, Coulombe-Huntington J, Yang F, Peng J, Weile J, Karras GI, Wang Y, Kov?cs IA, Kamburov A, Krykbaeva I, Lam MH, Tucker G, Khurana V, Sharma A, Liu YY, Yachie N, Zhong Q, Shen Y, Palagi A, San-Miguel A, Fan C, Balcha D, Dricot A, Jordan DM, Walsh JM, Shah AA, Yang X, Stoyanova AK, Leighton A, Calderwood MA, Jacob Y, Cusick ME, Salehi-Ashtiani K, Whitesell LJ, Sunyaev S, Berger B, Barab?si AL, Charloteaux B, Hill DE, Hao T, Roth FP, Xia Y, Walhout AJM, Lindquist S, Vidal M
Cell161(3)647-660(2015 Apr)

Mitochondrial EFTs defects in juvenile-onset Leigh disease, ataxia, neuropathy, and optic atrophy.
Ahola S, Isohanni P, Euro L, Brilhante V, Palotie A, Pihko H, L?nnqvist T, Lehtonen T, Laine J, Tyynismaa H, Suomalainen A
Neurology83(8)743-51(2014 Aug)

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Baltz AG, Munschauer M, Schwanh?usser B, Vasile A, Murakawa Y, Schueler M, Youngs N, Penfold-Brown D, Drew K, Milek M, Wyler E, Bonneau R, Selbach M, Dieterich C, Landthaler M
Molecular cell46(5)674-90(2012 Jun)

Mutation in the mitochondrial translation elongation factor EFTs results in severe infantile liver failure.
Vedrenne V, Galmiche L, Chretien D, de Lonlay P, Munnich A, R?tig A
Journal of hepatology56(1)294-7(2012 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Different TSFM mutations can produce the same or very different clinical phenotypes, going from abortions to moderately severe presentations. On the other hand, the same TSFM mutation can also produce same or different phenotypes within the same range of presentations, therefore suggesting the involvement of unknown factors.
Title: Molecular-genetic characterization and rescue of a TSFM mutation causing childhood-onset ataxia and nonobstructive cardiomyopathy.

show that in addition to early-onset cardiomyopathy, TSFM mutations should be considered in childhood and juvenile encephalopathies with optic and/or peripheral neuropathy, ataxia, or Leigh disease
Title: Mitochondrial EFTs defects in juvenile-onset Leigh disease, ataxia, neuropathy, and optic atrophy.

identified a homozygous mutation changing a highly conserved arginine into a tryptophan (R312W) in a kindred with intrauterine growth retardation, neonatal lactic acidosis, liver dysfunction and multiple respiratory chain deficiency in muscle
Title: Mutation in the mitochondrial translation elongation factor EFTs results in severe infantile liver failure.

Observational study of gene-disease association. (HuGE Navigator)
Title: Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.

Molecular modeling showed that the Arg333Trp substitution disrupts local subdomain structure and the dimerization interface.
Title: Distinct clinical phenotypes associated with a mutation in the mitochondrial translation elongation factor EFTs.

The following TSFM gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TSFM cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu55593	NM_001172696.2 Latest version!	Homo sapiens Ts translation elongation factor, mitochondrial (TSFM), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu09462	NM_001172695.2 Latest version!	Homo sapiens Ts translation elongation factor, mitochondrial (TSFM), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OHu19044	NM_001172697.1 Latest version!	Homo sapiens Ts translation elongation factor, mitochondrial (TSFM), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
OHu55812	NM_005726.6 Latest version!	Homo sapiens Ts translation elongation factor, mitochondrial (TSFM), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
View Old Accession Versions >>
OHu09462	NM_001172695.1	Homo sapiens Ts translation elongation factor, mitochondrial (TSFM), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OHu55593	NM_001172696.1	Homo sapiens Ts translation elongation factor, mitochondrial (TSFM), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu55812	NM_005726.5	Homo sapiens Ts translation elongation factor, mitochondrial (TSFM), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TSFM ( NM_001172696.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001166167.1 Homo sapiens Ts translation elongation factor, mitochondrial (TSFM), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu55593
Clone ID Related Accession (Same CDS sequence)	NM_001172696.2 , NM_001172696.1
Accession Version	NM_001172696.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1041bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Homo sapiens(human)
Product	elongation factor Ts, mitochondrial isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC022862.1 and AK304621.1. On Jun 2, 2019 this sequence version replaced NM_001172696.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC022862.1, BE741973.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2144335 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTCGCTGC TGCGGTCGCT GCGCGTGTTT CTGGTCGCGC GGACCGGGAG CTACCCGGCT 
GGGTCTCTTC TGCGTCAGTC GCCCCAGCCA AGGCACACAT TTTATGCTGG GCCCCGTCTG 
TCTGCCTCGG CCTCCAGCAA GGAGCTCCTC ATGAAGCTGC GGCGGAAAAC AGGCTACTCC 
TTTGTAAATT GCAAGAAAGC TCTGGAGACT TGTGGCGGGG ACCTCAAACA GGCAGAGATC 
TGGCTCCACA AGGAGGCCCA GAAGGAGGGC TGGAGCAAAG CTGCCAAGCT CCAAGGGAGG 
AAGACCAAAG AAGGCCTGAT TGGGCTGTTG CAGGAAGGAA ACACAACTGT ATTAGTAGAG 
GTAAACTGTG AGACAGATTT TGTTTCTAGA AATTTAAAAT TTCAACTGTT GGTCCAGCAA 
GTAGCCCTTG GAACCATGAT GCATTGTCAG ACCCTAAAGG ATCAACCCTC TGCATACAGT 
AAAGTGCAGT GGCTCACGCC TGTAAACCTA GCACTTTGGG AGGCTGAAGC AGGTGGATCA 
CTTGAGGGTT TCTTGAATTC CTCTGAGCTT TCTGGACTTC CAGCTGGGCC TGACAGAGAA 
GGCTCACTCA AGGATCAGTT GGCTTTAGCA ATTGGAAAAC TGGGAGAAAA CATGATTCTT 
AAACGAGCTG CATGGGTGAA GGTGCCATCT GGGTTCTACG TTGGCTCTTA TGTCCACGGA 
GCAATGCAGA GTCCCTCACT TCACAAGCTG GTGCTGGGGA AGTATGGGGC CCTGGTCATC 
TGTGAGACGT CTGAACAGAA AACAAACCTT GAAGACGTTG GCCGCCGCCT TGGGCAGCAT 
GTGGTGGGCA TGGCCCCCCT CTCTGTTGGC TCCCTGGACG ATGAGCCTGG GGGAGAGGCA 
GAGACTAAGA TGCTGTCCCA GCCGTATTTG CTGGATCCCT CCATTACCTT GGGGCAGTAT 
GTGCAGCCTC AGGGGGTGTC GGTAGTAGAC TTTGTGCGGT TTGAATGTGG AGAAGGTGAA 
GAGGCAGCAG AAACTGAATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001166167.1
CDS	16..1056
Translation	MSLLRSLRVFLVARTGSYPAGSLLRQSPQPRHTFYAGPRLSASASSKELLMKLRRKTGYSFVNCKKALETCGGDLKQAEIWLHKEAQKEGWSKAAKLQGRKTKEGLIGLLQEGNTTVLVEVNCETDFVSRNLKFQLLVQQVALGTMMHCQTLKDQPSAYSKVQWLTPVNLALWEAEAGGSLEGFLNSSELSGLPAGPDREGSLKDQLALAIGKLGENMILKRAAWVKVPSGFYVGSYVHGAMQSPSLHKLVLGKYGALVICETSEQKTNLEDVGRRLGQHVVGMAPLSVGSLDDEPGGEAETKMLSQPYLLDPSITLGQYVQPQGVSVVDFVRFECGEGEEAAETE

Target ORF information:

RefSeq Version	NM_001172696.2
Organism	Homo sapiens(human)
Definition	Homo sapiens Ts translation elongation factor, mitochondrial (TSFM), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001172696.2

ORF Insert Sequence:

ATGTCGCTGC TGCGGTCGCT GCGCGTGTTT CTGGTCGCGC GGACCGGGAG CTACCCGGCT 
GGGTCTCTTC TGCGTCAGTC GCCCCAGCCA AGGCACACAT TTTATGCTGG GCCCCGTCTG 
TCTGCCTCGG CCTCCAGCAA GGAGCTCCTC ATGAAGCTGC GGCGGAAAAC AGGCTACTCC 
TTTGTAAATT GCAAGAAAGC TCTGGAGACT TGTGGCGGGG ACCTCAAACA GGCAGAGATC 
TGGCTCCACA AGGAGGCCCA GAAGGAGGGC TGGAGCAAAG CTGCCAAGCT CCAAGGGAGG 
AAGACCAAAG AAGGCCTGAT TGGGCTGTTG CAGGAAGGAA ACACAACTGT ATTAGTAGAG 
GTAAACTGTG AGACAGATTT TGTTTCTAGA AATTTAAAAT TTCAACTGTT GGTCCAGCAA 
GTAGCCCTTG GAACCATGAT GCATTGTCAG ACCCTAAAGG ATCAACCCTC TGCATACAGT 
AAAGTGCAGT GGCTCACGCC TGTAAACCTA GCACTTTGGG AGGCTGAAGC AGGTGGATCA 
CTTGAGGGTT TCTTGAATTC CTCTGAGCTT TCTGGACTTC CAGCTGGGCC TGACAGAGAA 
GGCTCACTCA AGGATCAGTT GGCTTTAGCA ATTGGAAAAC TGGGAGAAAA CATGATTCTT 
AAACGAGCTG CATGGGTGAA GGTGCCATCT GGGTTCTACG TTGGCTCTTA TGTCCACGGA 
GCAATGCAGA GTCCCTCACT TCACAAGCTG GTGCTGGGGA AGTATGGGGC CCTGGTCATC 
TGTGAGACGT CTGAACAGAA AACAAACCTT GAAGACGTTG GCCGCCGCCT TGGGCAGCAT 
GTGGTGGGCA TGGCCCCCCT CTCTGTTGGC TCCCTGGACG ATGAGCCTGG GGGAGAGGCA 
GAGACTAAGA TGCTGTCCCA GCCGTATTTG CTGGATCCCT CCATTACCTT GGGGCAGTAT 
GTGCAGCCTC AGGGGGTGTC GGTAGTAGAC TTTGTGCGGT TTGAATGTGG AGAAGGTGAA 
GAGGCAGCAG AAACTGAATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSFM ( NM_001172696.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001166167.1 Homo sapiens Ts translation elongation factor, mitochondrial (TSFM), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu55593
Clone ID Related Accession (Same CDS sequence)	NM_001172696.2 , NM_001172696.1
Accession Version	NM_001172696.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1041bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-10-20
Organism	Homo sapiens(human)
Product	elongation factor Ts, mitochondrial isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB448773.1, BC022862.1 and AK304621.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC022862.1, BE741973.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2144335 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTCGCTGC TGCGGTCGCT GCGCGTGTTT CTGGTCGCGC GGACCGGGAG CTACCCGGCT 
GGGTCTCTTC TGCGTCAGTC GCCCCAGCCA AGGCACACAT TTTATGCTGG GCCCCGTCTG 
TCTGCCTCGG CCTCCAGCAA GGAGCTCCTC ATGAAGCTGC GGCGGAAAAC AGGCTACTCC 
TTTGTAAATT GCAAGAAAGC TCTGGAGACT TGTGGCGGGG ACCTCAAACA GGCAGAGATC 
TGGCTCCACA AGGAGGCCCA GAAGGAGGGC TGGAGCAAAG CTGCCAAGCT CCAAGGGAGG 
AAGACCAAAG AAGGCCTGAT TGGGCTGTTG CAGGAAGGAA ACACAACTGT ATTAGTAGAG 
GTAAACTGTG AGACAGATTT TGTTTCTAGA AATTTAAAAT TTCAACTGTT GGTCCAGCAA 
GTAGCCCTTG GAACCATGAT GCATTGTCAG ACCCTAAAGG ATCAACCCTC TGCATACAGT 
AAAGTGCAGT GGCTCACGCC TGTAAACCTA GCACTTTGGG AGGCTGAAGC AGGTGGATCA 
CTTGAGGGTT TCTTGAATTC CTCTGAGCTT TCTGGACTTC CAGCTGGGCC TGACAGAGAA 
GGCTCACTCA AGGATCAGTT GGCTTTAGCA ATTGGAAAAC TGGGAGAAAA CATGATTCTT 
AAACGAGCTG CATGGGTGAA GGTGCCATCT GGGTTCTACG TTGGCTCTTA TGTCCACGGA 
GCAATGCAGA GTCCCTCACT TCACAAGCTG GTGCTGGGGA AGTATGGGGC CCTGGTCATC 
TGTGAGACGT CTGAACAGAA AACAAACCTT GAAGACGTTG GCCGCCGCCT TGGGCAGCAT 
GTGGTGGGCA TGGCCCCCCT CTCTGTTGGC TCCCTGGACG ATGAGCCTGG GGGAGAGGCA 
GAGACTAAGA TGCTGTCCCA GCCGTATTTG CTGGATCCCT CCATTACCTT GGGGCAGTAT 
GTGCAGCCTC AGGGGGTGTC GGTAGTAGAC TTTGTGCGGT TTGAATGTGG AGAAGGTGAA 
GAGGCAGCAG AAACTGAATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001166167.1
CDS	58..1098
Translation	MSLLRSLRVFLVARTGSYPAGSLLRQSPQPRHTFYAGPRLSASASSKELLMKLRRKTGYSFVNCKKALETCGGDLKQAEIWLHKEAQKEGWSKAAKLQGRKTKEGLIGLLQEGNTTVLVEVNCETDFVSRNLKFQLLVQQVALGTMMHCQTLKDQPSAYSKVQWLTPVNLALWEAEAGGSLEGFLNSSELSGLPAGPDREGSLKDQLALAIGKLGENMILKRAAWVKVPSGFYVGSYVHGAMQSPSLHKLVLGKYGALVICETSEQKTNLEDVGRRLGQHVVGMAPLSVGSLDDEPGGEAETKMLSQPYLLDPSITLGQYVQPQGVSVVDFVRFECGEGEEAAETE

Target ORF information:

RefSeq Version	NM_001172696.1
Organism	Homo sapiens(human)
Definition	Homo sapiens Ts translation elongation factor, mitochondrial (TSFM), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001172696.1

ORF Insert Sequence:

ATGTCGCTGC TGCGGTCGCT GCGCGTGTTT CTGGTCGCGC GGACCGGGAG CTACCCGGCT 
GGGTCTCTTC TGCGTCAGTC GCCCCAGCCA AGGCACACAT TTTATGCTGG GCCCCGTCTG 
TCTGCCTCGG CCTCCAGCAA GGAGCTCCTC ATGAAGCTGC GGCGGAAAAC AGGCTACTCC 
TTTGTAAATT GCAAGAAAGC TCTGGAGACT TGTGGCGGGG ACCTCAAACA GGCAGAGATC 
TGGCTCCACA AGGAGGCCCA GAAGGAGGGC TGGAGCAAAG CTGCCAAGCT CCAAGGGAGG 
AAGACCAAAG AAGGCCTGAT TGGGCTGTTG CAGGAAGGAA ACACAACTGT ATTAGTAGAG 
GTAAACTGTG AGACAGATTT TGTTTCTAGA AATTTAAAAT TTCAACTGTT GGTCCAGCAA 
GTAGCCCTTG GAACCATGAT GCATTGTCAG ACCCTAAAGG ATCAACCCTC TGCATACAGT 
AAAGTGCAGT GGCTCACGCC TGTAAACCTA GCACTTTGGG AGGCTGAAGC AGGTGGATCA 
CTTGAGGGTT TCTTGAATTC CTCTGAGCTT TCTGGACTTC CAGCTGGGCC TGACAGAGAA 
GGCTCACTCA AGGATCAGTT GGCTTTAGCA ATTGGAAAAC TGGGAGAAAA CATGATTCTT 
AAACGAGCTG CATGGGTGAA GGTGCCATCT GGGTTCTACG TTGGCTCTTA TGTCCACGGA 
GCAATGCAGA GTCCCTCACT TCACAAGCTG GTGCTGGGGA AGTATGGGGC CCTGGTCATC 
TGTGAGACGT CTGAACAGAA AACAAACCTT GAAGACGTTG GCCGCCGCCT TGGGCAGCAT 
GTGGTGGGCA TGGCCCCCCT CTCTGTTGGC TCCCTGGACG ATGAGCCTGG GGGAGAGGCA 
GAGACTAAGA TGCTGTCCCA GCCGTATTTG CTGGATCCCT CCATTACCTT GGGGCAGTAT 
GTGCAGCCTC AGGGGGTGTC GGTAGTAGAC TTTGTGCGGT TTGAATGTGG AGAAGGTGAA 
GAGGCAGCAG AAACTGAATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSFM ( NM_001172695.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001166166.1 Homo sapiens Ts translation elongation factor, mitochondrial (TSFM), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu09462
Clone ID Related Accession (Same CDS sequence)	NM_001172695.1 , NM_001172695.2
Accession Version	NM_001172695.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 504bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-10-20
Organism	Homo sapiens(human)
Product	elongation factor Ts, mitochondrial isoform 3 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB448773.1 and AK304621.1. Transcript Variant: This variant (3) lacks two exons in the coding region, which results in a frameshift and an early stop codon, compared to variant 1. The encoded isoform (3) is shorter and has a distinct C-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK304621.1, SRR1803613.235640.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTCGCTGC TGCGGTCGCT GCGCGTGTTT CTGGTCGCGC GGACCGGGAG CTACCCGGCT 
GGGTCTCTTC TGCGTCAGTC GCCCCAGCCA AGGCACACAT TTTATGCTGG GCCCCGTCTG 
TCTGCCTCGG CCTCCAGCAA GGAGCTCCTC ATGAAGCTGC GGCGGAAAAC AGGCTACTCC 
TTTGTAAATT GCAAGAAAGC TCTGGAGACT TGTGGCGGGG ACCTCAAACA GGCAGAGATC 
TGGCTCCACA AGGAGGCCCA GAAGGAGGGC TGGAGCAAAG CTGCCAAGCT CCAAGGGAGG 
AAGACCAAAG AAGGCCTGAT TGGGCTGTTG CAGGAAGGAA ACACAACTGT ATTAGTAGAG 
GTAAACTGTG AGACAGATTT TGTTTCTAGA AATTTAAAAT TTCAACTGTT GGTCCAGCAA 
GTAGCCCTTG GAACCATGAT GCATTGTCAG ACCCTAAAGG ATCAACCCTC TGCATACAGT 
AAAGAAAACT GGGAGAAAAC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001166166.1
CDS	58..561
Translation	MSLLRSLRVFLVARTGSYPAGSLLRQSPQPRHTFYAGPRLSASASSKELLMKLRRKTGYSFVNCKKALETCGGDLKQAEIWLHKEAQKEGWSKAAKLQGRKTKEGLIGLLQEGNTTVLVEVNCETDFVSRNLKFQLLVQQVALGTMMHCQTLKDQPSAYSKENWEKT

Target ORF information:

RefSeq Version	NM_001172695.1
Organism	Homo sapiens(human)
Definition	Homo sapiens Ts translation elongation factor, mitochondrial (TSFM), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001172695.1

ORF Insert Sequence:

ATGTCGCTGC TGCGGTCGCT GCGCGTGTTT CTGGTCGCGC GGACCGGGAG CTACCCGGCT 
GGGTCTCTTC TGCGTCAGTC GCCCCAGCCA AGGCACACAT TTTATGCTGG GCCCCGTCTG 
TCTGCCTCGG CCTCCAGCAA GGAGCTCCTC ATGAAGCTGC GGCGGAAAAC AGGCTACTCC 
TTTGTAAATT GCAAGAAAGC TCTGGAGACT TGTGGCGGGG ACCTCAAACA GGCAGAGATC 
TGGCTCCACA AGGAGGCCCA GAAGGAGGGC TGGAGCAAAG CTGCCAAGCT CCAAGGGAGG 
AAGACCAAAG AAGGCCTGAT TGGGCTGTTG CAGGAAGGAA ACACAACTGT ATTAGTAGAG 
GTAAACTGTG AGACAGATTT TGTTTCTAGA AATTTAAAAT TTCAACTGTT GGTCCAGCAA 
GTAGCCCTTG GAACCATGAT GCATTGTCAG ACCCTAAAGG ATCAACCCTC TGCATACAGT 
AAAGAAAACT GGGAGAAAAC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSFM ( NM_001172695.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001166166.1 Homo sapiens Ts translation elongation factor, mitochondrial (TSFM), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu09462
Clone ID Related Accession (Same CDS sequence)	NM_001172695.1 , NM_001172695.2
Accession Version	NM_001172695.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 504bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-08
Organism	Homo sapiens(human)
Product	elongation factor Ts, mitochondrial isoform 3 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK304621.1. On Jun 2, 2019 this sequence version replaced NM_001172695.1. Transcript Variant: This variant (3) lacks two exons in the coding region, which results in a frameshift and an early stop codon, compared to variant 1. The encoded isoform (3) is shorter and has a distinct C-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK304621.1, SRR1803613.235640.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTCGCTGC TGCGGTCGCT GCGCGTGTTT CTGGTCGCGC GGACCGGGAG CTACCCGGCT 
GGGTCTCTTC TGCGTCAGTC GCCCCAGCCA AGGCACACAT TTTATGCTGG GCCCCGTCTG 
TCTGCCTCGG CCTCCAGCAA GGAGCTCCTC ATGAAGCTGC GGCGGAAAAC AGGCTACTCC 
TTTGTAAATT GCAAGAAAGC TCTGGAGACT TGTGGCGGGG ACCTCAAACA GGCAGAGATC 
TGGCTCCACA AGGAGGCCCA GAAGGAGGGC TGGAGCAAAG CTGCCAAGCT CCAAGGGAGG 
AAGACCAAAG AAGGCCTGAT TGGGCTGTTG CAGGAAGGAA ACACAACTGT ATTAGTAGAG 
GTAAACTGTG AGACAGATTT TGTTTCTAGA AATTTAAAAT TTCAACTGTT GGTCCAGCAA 
GTAGCCCTTG GAACCATGAT GCATTGTCAG ACCCTAAAGG ATCAACCCTC TGCATACAGT 
AAAGAAAACT GGGAGAAAAC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001166166.1
CDS	16..519
Translation	MSLLRSLRVFLVARTGSYPAGSLLRQSPQPRHTFYAGPRLSASASSKELLMKLRRKTGYSFVNCKKALETCGGDLKQAEIWLHKEAQKEGWSKAAKLQGRKTKEGLIGLLQEGNTTVLVEVNCETDFVSRNLKFQLLVQQVALGTMMHCQTLKDQPSAYSKENWEKT

Target ORF information:

RefSeq Version	NM_001172695.2
Organism	Homo sapiens(human)
Definition	Homo sapiens Ts translation elongation factor, mitochondrial (TSFM), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001172695.2

ORF Insert Sequence:

ATGTCGCTGC TGCGGTCGCT GCGCGTGTTT CTGGTCGCGC GGACCGGGAG CTACCCGGCT 
GGGTCTCTTC TGCGTCAGTC GCCCCAGCCA AGGCACACAT TTTATGCTGG GCCCCGTCTG 
TCTGCCTCGG CCTCCAGCAA GGAGCTCCTC ATGAAGCTGC GGCGGAAAAC AGGCTACTCC 
TTTGTAAATT GCAAGAAAGC TCTGGAGACT TGTGGCGGGG ACCTCAAACA GGCAGAGATC 
TGGCTCCACA AGGAGGCCCA GAAGGAGGGC TGGAGCAAAG CTGCCAAGCT CCAAGGGAGG 
AAGACCAAAG AAGGCCTGAT TGGGCTGTTG CAGGAAGGAA ACACAACTGT ATTAGTAGAG 
GTAAACTGTG AGACAGATTT TGTTTCTAGA AATTTAAAAT TTCAACTGTT GGTCCAGCAA 
GTAGCCCTTG GAACCATGAT GCATTGTCAG ACCCTAAAGG ATCAACCCTC TGCATACAGT 
AAAGAAAACT GGGAGAAAAC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSFM ( NM_001172697.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001166168.1 Homo sapiens Ts translation elongation factor, mitochondrial (TSFM), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu19044
Clone ID Related Accession (Same CDS sequence)	NM_001172697.1
Accession Version	NM_001172697.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 648bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-03
Organism	Homo sapiens(human)
Product	elongation factor Ts, mitochondrial isoform 4 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB448773.1 and AK308981.1. This sequence is a reference standard in the RefSeqGene project. Transcript Variant: This variant (4) differs in the 3' UTR and has multiple differences in the coding region, compared to variant 1, one of which results in a translational frameshift. The resulting protein (isoform 4) has a distinct C-terminus and is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK308981.1, SRR1803612.21018.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END##

ATGTCGCTGC TGCGGTCGCT GCGCGTGTTT CTGGTCGCGC GGACCGGGAG CTACCCGGCT 
GGGTCTCTTC TGCGTCAGTC GCCCCAGCCA AGGCACACAT TTTATGCTGG GCCCCGTCTG 
TCTGCCTCGG CCTCCAGCAA GGAGCTCCTC ATGAAGCTGC GGCGGAAAAC AGGCTACTCC 
TTTGTAAATT GCAAGAAAGC TCTGGAGACT TGTGGCGGGG ACCTCAAACA GGCAGAGATC 
TGGCTCCACA AGGAGGCCCA GAAGGAGGGC TGGAGCAAAG CTGCCAAGCT CCAAGGGAGG 
AAGACCAAAG AAGGCCTGAT TGGGCTGTTG CAGGAAGGAA ACACAACTGT ATTAGTAGAG 
GTAAACTGTG AGACAGATTT TGTTTCTAGA AATTTAAAAT TTCAACTGTT GGTCCAGCAA 
GTAGCCCTTG GAACCATGAT GCATTGTCAG ACCCTAAAGG ATCAACCCTC TGCATACAGT 
AAAGGTTTCT TGAATTCCTC TGAGCTTTCT GGACTTCCAG CTGGGCCTGA CAGAGAAGGC 
TCACTCAAGG ATCAGTTGGC TTTAGCAATT GGTCTGCCTT TCCAAATTGG AGCTGTCTCT 
GAACTTCAGC TTCCTGAATC CAACTTCTTG CAGAACACCT CCTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001166168.1
CDS	58..705
Translation	MSLLRSLRVFLVARTGSYPAGSLLRQSPQPRHTFYAGPRLSASASSKELLMKLRRKTGYSFVNCKKALETCGGDLKQAEIWLHKEAQKEGWSKAAKLQGRKTKEGLIGLLQEGNTTVLVEVNCETDFVSRNLKFQLLVQQVALGTMMHCQTLKDQPSAYSKGFLNSSELSGLPAGPDREGSLKDQLALAIGLPFQIGAVSELQLPESNFLQNTSS

Target ORF information:

RefSeq Version	NM_001172697.1
Organism	Homo sapiens(human)
Definition	Homo sapiens Ts translation elongation factor, mitochondrial (TSFM), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001172697.1

ORF Insert Sequence:

ATGTCGCTGC TGCGGTCGCT GCGCGTGTTT CTGGTCGCGC GGACCGGGAG CTACCCGGCT 
GGGTCTCTTC TGCGTCAGTC GCCCCAGCCA AGGCACACAT TTTATGCTGG GCCCCGTCTG 
TCTGCCTCGG CCTCCAGCAA GGAGCTCCTC ATGAAGCTGC GGCGGAAAAC AGGCTACTCC 
TTTGTAAATT GCAAGAAAGC TCTGGAGACT TGTGGCGGGG ACCTCAAACA GGCAGAGATC 
TGGCTCCACA AGGAGGCCCA GAAGGAGGGC TGGAGCAAAG CTGCCAAGCT CCAAGGGAGG 
AAGACCAAAG AAGGCCTGAT TGGGCTGTTG CAGGAAGGAA ACACAACTGT ATTAGTAGAG 
GTAAACTGTG AGACAGATTT TGTTTCTAGA AATTTAAAAT TTCAACTGTT GGTCCAGCAA 
GTAGCCCTTG GAACCATGAT GCATTGTCAG ACCCTAAAGG ATCAACCCTC TGCATACAGT 
AAAGGTTTCT TGAATTCCTC TGAGCTTTCT GGACTTCCAG CTGGGCCTGA CAGAGAAGGC 
TCACTCAAGG ATCAGTTGGC TTTAGCAATT GGTCTGCCTT TCCAAATTGG AGCTGTCTCT 
GAACTTCAGC TTCCTGAATC CAACTTCTTG CAGAACACCT CCTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSFM ( NM_005726.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_005717.3 Homo sapiens Ts translation elongation factor, mitochondrial (TSFM), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu55812
Clone ID Related Accession (Same CDS sequence)	NM_005726.5 , NM_005726.6
Accession Version	NM_005726.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 978bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-21
Organism	Homo sapiens(human)
Product	elongation factor Ts, mitochondrial isoform 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB448773.1, AF110399.1 and AK304621.1. On Mar 16, 2010 this sequence version replaced NM_005726.4. Transcript Variant: This variant (2) lacks an in-frame exon in the coding region, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.147009.1, SRR1803612.245765.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTCGCTGC TGCGGTCGCT GCGCGTGTTT CTGGTCGCGC GGACCGGGAG CTACCCGGCT 
GGGTCTCTTC TGCGTCAGTC GCCCCAGCCA AGGCACACAT TTTATGCTGG GCCCCGTCTG 
TCTGCCTCGG CCTCCAGCAA GGAGCTCCTC ATGAAGCTGC GGCGGAAAAC AGGCTACTCC 
TTTGTAAATT GCAAGAAAGC TCTGGAGACT TGTGGCGGGG ACCTCAAACA GGCAGAGATC 
TGGCTCCACA AGGAGGCCCA GAAGGAGGGC TGGAGCAAAG CTGCCAAGCT CCAAGGGAGG 
AAGACCAAAG AAGGCCTGAT TGGGCTGTTG CAGGAAGGAA ACACAACTGT ATTAGTAGAG 
GTAAACTGTG AGACAGATTT TGTTTCTAGA AATTTAAAAT TTCAACTGTT GGTCCAGCAA 
GTAGCCCTTG GAACCATGAT GCATTGTCAG ACCCTAAAGG ATCAACCCTC TGCATACAGT 
AAAGGTTTCT TGAATTCCTC TGAGCTTTCT GGACTTCCAG CTGGGCCTGA CAGAGAAGGC 
TCACTCAAGG ATCAGTTGGC TTTAGCAATT GGAAAACTGG GAGAAAACAT GATTCTTAAA 
CGAGCTGCAT GGGTGAAGGT GCCATCTGGG TTCTACGTTG GCTCTTATGT CCACGGAGCA 
ATGCAGAGTC CCTCACTTCA CAAGCTGGTG CTGGGGAAGT ATGGGGCCCT GGTCATCTGT 
GAGACGTCTG AACAGAAAAC AAACCTTGAA GACGTTGGCC GCCGCCTTGG GCAGCATGTG 
GTGGGCATGG CCCCCCTCTC TGTTGGCTCC CTGGACGATG AGCCTGGGGG AGAGGCAGAG 
ACTAAGATGC TGTCCCAGCC GTATTTGCTG GATCCCTCCA TTACCTTGGG GCAGTATGTG 
CAGCCTCAGG GGGTGTCGGT AGTAGACTTT GTGCGGTTTG AATGTGGAGA AGGTGAAGAG 
GCAGCAGAAA CTGAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005717.3
CDS	58..1035
Translation	MSLLRSLRVFLVARTGSYPAGSLLRQSPQPRHTFYAGPRLSASASSKELLMKLRRKTGYSFVNCKKALETCGGDLKQAEIWLHKEAQKEGWSKAAKLQGRKTKEGLIGLLQEGNTTVLVEVNCETDFVSRNLKFQLLVQQVALGTMMHCQTLKDQPSAYSKGFLNSSELSGLPAGPDREGSLKDQLALAIGKLGENMILKRAAWVKVPSGFYVGSYVHGAMQSPSLHKLVLGKYGALVICETSEQKTNLEDVGRRLGQHVVGMAPLSVGSLDDEPGGEAETKMLSQPYLLDPSITLGQYVQPQGVSVVDFVRFECGEGEEAAETE

Target ORF information:

RefSeq Version	NM_005726.5
Organism	Homo sapiens(human)
Definition	Homo sapiens Ts translation elongation factor, mitochondrial (TSFM), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005726.5

ORF Insert Sequence:

ATGTCGCTGC TGCGGTCGCT GCGCGTGTTT CTGGTCGCGC GGACCGGGAG CTACCCGGCT 
GGGTCTCTTC TGCGTCAGTC GCCCCAGCCA AGGCACACAT TTTATGCTGG GCCCCGTCTG 
TCTGCCTCGG CCTCCAGCAA GGAGCTCCTC ATGAAGCTGC GGCGGAAAAC AGGCTACTCC 
TTTGTAAATT GCAAGAAAGC TCTGGAGACT TGTGGCGGGG ACCTCAAACA GGCAGAGATC 
TGGCTCCACA AGGAGGCCCA GAAGGAGGGC TGGAGCAAAG CTGCCAAGCT CCAAGGGAGG 
AAGACCAAAG AAGGCCTGAT TGGGCTGTTG CAGGAAGGAA ACACAACTGT ATTAGTAGAG 
GTAAACTGTG AGACAGATTT TGTTTCTAGA AATTTAAAAT TTCAACTGTT GGTCCAGCAA 
GTAGCCCTTG GAACCATGAT GCATTGTCAG ACCCTAAAGG ATCAACCCTC TGCATACAGT 
AAAGGTTTCT TGAATTCCTC TGAGCTTTCT GGACTTCCAG CTGGGCCTGA CAGAGAAGGC 
TCACTCAAGG ATCAGTTGGC TTTAGCAATT GGAAAACTGG GAGAAAACAT GATTCTTAAA 
CGAGCTGCAT GGGTGAAGGT GCCATCTGGG TTCTACGTTG GCTCTTATGT CCACGGAGCA 
ATGCAGAGTC CCTCACTTCA CAAGCTGGTG CTGGGGAAGT ATGGGGCCCT GGTCATCTGT 
GAGACGTCTG AACAGAAAAC AAACCTTGAA GACGTTGGCC GCCGCCTTGG GCAGCATGTG 
GTGGGCATGG CCCCCCTCTC TGTTGGCTCC CTGGACGATG AGCCTGGGGG AGAGGCAGAG 
ACTAAGATGC TGTCCCAGCC GTATTTGCTG GATCCCTCCA TTACCTTGGG GCAGTATGTG 
CAGCCTCAGG GGGTGTCGGT AGTAGACTTT GTGCGGTTTG AATGTGGAGA AGGTGAAGAG 
GCAGCAGAAA CTGAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSFM ( NM_005726.6 ) cDNA ORF clone, Homo sapiens(human) -> NP_005717.3 Homo sapiens Ts translation elongation factor, mitochondrial (TSFM), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu55812
Clone ID Related Accession (Same CDS sequence)	NM_005726.5 , NM_005726.6
Accession Version	NM_005726.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 978bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-27
Organism	Homo sapiens(human)
Product	elongation factor Ts, mitochondrial isoform 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB448773.1, AF110399.1 and AK304621.1. On Jun 2, 2019 this sequence version replaced NM_005726.5. Transcript Variant: This variant (2) lacks an in-frame exon in the coding region, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.1535.1, SRR1803611.186929.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta RefSeq Select criteria :: based on manual assertion, conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTCGCTGC TGCGGTCGCT GCGCGTGTTT CTGGTCGCGC GGACCGGGAG CTACCCGGCT 
GGGTCTCTTC TGCGTCAGTC GCCCCAGCCA AGGCACACAT TTTATGCTGG GCCCCGTCTG 
TCTGCCTCGG CCTCCAGCAA GGAGCTCCTC ATGAAGCTGC GGCGGAAAAC AGGCTACTCC 
TTTGTAAATT GCAAGAAAGC TCTGGAGACT TGTGGCGGGG ACCTCAAACA GGCAGAGATC 
TGGCTCCACA AGGAGGCCCA GAAGGAGGGC TGGAGCAAAG CTGCCAAGCT CCAAGGGAGG 
AAGACCAAAG AAGGCCTGAT TGGGCTGTTG CAGGAAGGAA ACACAACTGT ATTAGTAGAG 
GTAAACTGTG AGACAGATTT TGTTTCTAGA AATTTAAAAT TTCAACTGTT GGTCCAGCAA 
GTAGCCCTTG GAACCATGAT GCATTGTCAG ACCCTAAAGG ATCAACCCTC TGCATACAGT 
AAAGGTTTCT TGAATTCCTC TGAGCTTTCT GGACTTCCAG CTGGGCCTGA CAGAGAAGGC 
TCACTCAAGG ATCAGTTGGC TTTAGCAATT GGAAAACTGG GAGAAAACAT GATTCTTAAA 
CGAGCTGCAT GGGTGAAGGT GCCATCTGGG TTCTACGTTG GCTCTTATGT CCACGGAGCA 
ATGCAGAGTC CCTCACTTCA CAAGCTGGTG CTGGGGAAGT ATGGGGCCCT GGTCATCTGT 
GAGACGTCTG AACAGAAAAC AAACCTTGAA GACGTTGGCC GCCGCCTTGG GCAGCATGTG 
GTGGGCATGG CCCCCCTCTC TGTTGGCTCC CTGGACGATG AGCCTGGGGG AGAGGCAGAG 
ACTAAGATGC TGTCCCAGCC GTATTTGCTG GATCCCTCCA TTACCTTGGG GCAGTATGTG 
CAGCCTCAGG GGGTGTCGGT AGTAGACTTT GTGCGGTTTG AATGTGGAGA AGGTGAAGAG 
GCAGCAGAAA CTGAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005717.3
CDS	16..993
Translation	MSLLRSLRVFLVARTGSYPAGSLLRQSPQPRHTFYAGPRLSASASSKELLMKLRRKTGYSFVNCKKALETCGGDLKQAEIWLHKEAQKEGWSKAAKLQGRKTKEGLIGLLQEGNTTVLVEVNCETDFVSRNLKFQLLVQQVALGTMMHCQTLKDQPSAYSKGFLNSSELSGLPAGPDREGSLKDQLALAIGKLGENMILKRAAWVKVPSGFYVGSYVHGAMQSPSLHKLVLGKYGALVICETSEQKTNLEDVGRRLGQHVVGMAPLSVGSLDDEPGGEAETKMLSQPYLLDPSITLGQYVQPQGVSVVDFVRFECGEGEEAAETE

Target ORF information:

RefSeq Version	NM_005726.6
Organism	Homo sapiens(human)
Definition	Homo sapiens Ts translation elongation factor, mitochondrial (TSFM), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005726.6

ORF Insert Sequence:

ATGTCGCTGC TGCGGTCGCT GCGCGTGTTT CTGGTCGCGC GGACCGGGAG CTACCCGGCT 
GGGTCTCTTC TGCGTCAGTC GCCCCAGCCA AGGCACACAT TTTATGCTGG GCCCCGTCTG 
TCTGCCTCGG CCTCCAGCAA GGAGCTCCTC ATGAAGCTGC GGCGGAAAAC AGGCTACTCC 
TTTGTAAATT GCAAGAAAGC TCTGGAGACT TGTGGCGGGG ACCTCAAACA GGCAGAGATC 
TGGCTCCACA AGGAGGCCCA GAAGGAGGGC TGGAGCAAAG CTGCCAAGCT CCAAGGGAGG 
AAGACCAAAG AAGGCCTGAT TGGGCTGTTG CAGGAAGGAA ACACAACTGT ATTAGTAGAG 
GTAAACTGTG AGACAGATTT TGTTTCTAGA AATTTAAAAT TTCAACTGTT GGTCCAGCAA 
GTAGCCCTTG GAACCATGAT GCATTGTCAG ACCCTAAAGG ATCAACCCTC TGCATACAGT 
AAAGGTTTCT TGAATTCCTC TGAGCTTTCT GGACTTCCAG CTGGGCCTGA CAGAGAAGGC 
TCACTCAAGG ATCAGTTGGC TTTAGCAATT GGAAAACTGG GAGAAAACAT GATTCTTAAA 
CGAGCTGCAT GGGTGAAGGT GCCATCTGGG TTCTACGTTG GCTCTTATGT CCACGGAGCA 
ATGCAGAGTC CCTCACTTCA CAAGCTGGTG CTGGGGAAGT ATGGGGCCCT GGTCATCTGT 
GAGACGTCTG AACAGAAAAC AAACCTTGAA GACGTTGGCC GCCGCCTTGG GCAGCATGTG 
GTGGGCATGG CCCCCCTCTC TGTTGGCTCC CTGGACGATG AGCCTGGGGG AGAGGCAGAG 
ACTAAGATGC TGTCCCAGCC GTATTTGCTG GATCCCTCCA TTACCTTGGG GCAGTATGTG 
CAGCCTCAGG GGGTGTCGGT AGTAGACTTT GTGCGGTTTG AATGTGGAGA AGGTGAAGAG 
GCAGCAGAAA CTGAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Molecular-genetic characterization and rescue of a TSFM mutation causing childhood-onset ataxia and nonobstructive cardiomyopathy.
European journal of human genetics : EJHG25(1)153-156(2016 01)
Emperador S,Bayona-Bafaluy MP,Fern?ndez-Marmiesse A,Pineda M,Felgueroso B,L?pez-Gallardo E,Artuch R,Roca I,Ruiz-Pesini E,Couce ML,Montoya J

Widespread macromolecular interaction perturbations in human genetic disorders.
Cell161(3)647-660(2015 Apr)
Sahni N,Yi S,Taipale M,Fuxman Bass JI,Coulombe-Huntington J,Yang F,Peng J,Weile J,Karras GI,Wang Y,Kov?cs IA,Kamburov A,Krykbaeva I,Lam MH,Tucker G,Khurana V,Sharma A,Liu YY,Yachie N,Zhong Q,Shen Y,Palagi A,San-Miguel A,Fan C,Balcha D,Dricot A,Jordan DM,Walsh JM,Shah AA,Yang X,Stoyanova AK,Leighton A,Calderwood MA,Jacob Y,Cusick ME,Salehi-Ashtiani K,Whitesell LJ,Sunyaev S,Berger B,Barab?si AL,Charloteaux B,Hill DE,Hao T,Roth FP,Xia Y,Walhout AJM,Lindquist S,Vidal M

Mitochondrial EFTs defects in juvenile-onset Leigh disease, ataxia, neuropathy, and optic atrophy.
Neurology83(8)743-51(2014 Aug)
Ahola S,Isohanni P,Euro L,Brilhante V,Palotie A,Pihko H,L?nnqvist T,Lehtonen T,Laine J,Tyynismaa H,Suomalainen A

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Molecular cell46(5)674-90(2012 Jun)
Baltz AG,Munschauer M,Schwanh?usser B,Vasile A,Murakawa Y,Schueler M,Youngs N,Penfold-Brown D,Drew K,Milek M,Wyler E,Bonneau R,Selbach M,Dieterich C,Landthaler M

Mutation in the mitochondrial translation elongation factor EFTs results in severe infantile liver failure.
Journal of hepatology56(1)294-7(2012 Jan)
Vedrenne V,Galmiche L,Chretien D,de Lonlay P,Munnich A,R?tig A

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TSFM cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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