FARSB cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	FARSB
Entrez Gene ID	10056
Full Name	phenylalanyl-tRNA synthetase beta subunit
Synonyms	FARSLB,FRSB,HSPC173,PheHB,PheRS
General protein information	Preferred Names phenylalanyl-tRNA synthetase beta subunit Names phenylalanine--tRNA ligase beta subunit phenylalanine tRNA ligase 1, beta, cytoplasmic phenylalanine-tRNA ligase beta chain phenylalanine-tRNA synthetase-like, beta subunit phenylalanyl-tRNA synthetase beta chain phenylalanyl-tRNA synthetase-like, beta subunit
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 2 Map Location: 2q36.1
Summary	This gene encodes a highly conserved enzyme that belongs to the aminoacyl-tRNA synthetase class IIc subfamily. This enzyme comprises the regulatory beta subunits that form a tetramer with two catalytic alpha subunits. In the presence of ATP, this tetramer is responsible for attaching L-phenylalanine to the terminal adenosine of the appropriate tRNA. A pseudogene located on chromosome 10 has been identified. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2015].
Disorder MIM:	609690

mRNA and Protein(s)

mRNA	Protein	Name
XM_017003110.2	XP_016858599.1	phenylalanine--tRNA ligase beta subunit isoform X1
NM_005687.4	NP_005678.3	phenylalanine--tRNA ligase beta subunit
NM_005687.5	NP_005678.3	phenylalanine--tRNA ligase beta subunit
XM_006712169.2	XP_006712232.1	phenylalanine--tRNA ligase beta subunit isoform X1
XM_011510466.2	XP_011508768.1	phenylalanine--tRNA ligase beta subunit isoform X1
XM_017003110.1	XP_016858599.1	phenylalanine--tRNA ligase beta subunit isoform X1
XM_024452492.1	XP_024308260.1	phenylalanine--tRNA ligase beta subunit isoform X1

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	farsb	NP_001007769.1
	FARSB	XP_002808073.1
Drosophila melanogaster (fruit fly)	CG5706	NP_651237.1
Arabidopsis thaliana (thale cress)	AT1G72550	NP_177399.1
Oryza sativa (rice)	Os05g0558900	NP_001056297.1
Canis lupus familiaris (dog)	FARSB	XP_536085.2
Pan troglodytes (chimpanzee)	FARSB	NP_001233467.1
Gallus gallus (chicken)	FARSB	NP_001006543.1
Caenorhabditis elegans (roundworm)	fars-3	NP_495785.1
Saccharomyces cerevisiae (baker's yeast)	FRS1	NP_013161.1
Kluyveromyces lactis	KLLA0D05137g	XP_453289.1
Eremothecium gossypii	AGOS_ACR219W	NP_983621.2
Schizosaccharomyces pombe (fission yeast)	frs1	NP_594442.1
Homo sapiens (human)	FARSB	NP_005678.3
Bos taurus (cattle)	FARSB	NP_001098491.1
Mus musculus (house mouse)	Farsb	NP_001265004.1
Rattus norvegicus (Norway rat)	Farsb	NP_001004252.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003517	XP_003436507.1
Magnaporthe oryzae (rice blast fungus)	MGG_00876	XP_003718093.1
Neurospora crassa	NCU08888	XP_958826.2
Xenopus tropicalis (tropical clawed frog)	farsb	NP_001015711.1

Gene Ontology
Bibliography

Related articles in PubMed

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

Use of germline polymorphisms in predicting concurrent chemoradiotherapy response in esophageal cancer.
Chen PC, Chen YC, Lai LC, Tsai MH, Chen SK, Yang PW, Lee YC, Hsiao CK, Lee JM, Chuang EY
International journal of radiation oncology, biology, physics82(5)1996-2003(2012 Apr)

Crystal structure of human mitochondrial PheRS complexed with tRNA(Phe) in the active "open" state.
Klipcan L, Moor N, Finarov I, Kessler N, Sukhanova M, Safro MG
Journal of molecular biology415(3)527-37(2012 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Human PheRS recognizes C74, the G1-C72 base pair, and the "discriminator" base A73, proposed to contribute to tRNA(Phe) identity in the yeast mitochondrial enzyme.
Title: Crystal structure of human mitochondrial PheRS complexed with tRNA(Phe) in the active "open" state.

Clinical trial of gene-disease association and gene-environment interaction. (HuGE Navigator)
Title: Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score.

Structure of human cytosolic phenylalanyl-tRNA synthetase: evidence for kingdom-specific design of the active sites and tRNA binding patterns
Title: Structure of human cytosolic phenylalanyl-tRNA synthetase: evidence for kingdom-specific design of the active sites and tRNA binding patterns.

The following FARSB gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the FARSB cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu05107	NM_005687.5 Latest version!	Homo sapiens phenylalanyl-tRNA synthetase subunit beta (FARSB), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu35721	XM_024452492.1 Latest version!	Homo sapiens phenylalanyl-tRNA synthetase subunit beta (FARSB), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu35721	XM_006712169.2 Latest version!	Homo sapiens phenylalanyl-tRNA synthetase subunit beta (FARSB), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu35721	XM_011510466.2 Latest version!	Homo sapiens phenylalanyl-tRNA synthetase subunit beta (FARSB), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu35721	XM_017003110.2 Latest version!	Homo sapiens phenylalanyl-tRNA synthetase subunit beta (FARSB), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
View Old Accession Versions >>
OHu05107	NM_005687.4	Homo sapiens phenylalanyl-tRNA synthetase subunit beta (FARSB), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu35721	XM_017003110.1	Homo sapiens phenylalanyl-tRNA synthetase beta subunit (FARSB), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

FARSB ( NM_005687.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_005678.3 Homo sapiens phenylalanyl-tRNA synthetase subunit beta (FARSB), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu05107
Clone ID Related Accession (Same CDS sequence)	NM_005687.4 , NM_005687.5
Accession Version	NM_005687.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1770bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Homo sapiens(human)
Product	phenylalanine--tRNA ligase beta subunit
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC104772.3, BG721840.1, AF042346.1 and AC097461.3. On Jan 6, 2015 this sequence version replaced NM_005687.3. Transcript Variant: This variant (1) represents the shorter transcript and encodes the functional protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.139210.1, SRR1803616.188627.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCGACTG TCAGCGTGAA GCGTGATCTG CTCTTCCAAG CCCTGGGCCG CACCTACACT 
GACGAAGAAT TTGATGAACT ATGTTTTGAA TTTGGTCTGG AGCTTGATGA AATTACATCT 
GAGAAGGAAA TAATAAGTAA AGAACAAGGT AATGTAAAGG CAGCAGGAGC CTCTGATGTT 
GTTCTTTACA AAATTGACGT CCCTGCCAAT AGATATGATC TCCTGTGTCT GGAAGGATTG 
GTTCGAGGAC TTCAGGTCTT CAAAGAAAGG ATAAAGGCTC CAGTGTATAA ACGGGTAATG 
CCTGATGGAA AAATCCAGAA ATTGATTATC ACAGAAGAGA CAGCTAAGAT ACGTCCTTTT 
GCGGTAGCAG CAGTTCTCCG TAATATAAAG TTTACTAAAG ATCGATATGA CAGCTTCATT 
GAACTTCAGG AGAAATTACA TCAGAATATT TGCAGGAAAA GAGCACTGGT TGCCATTGGT 
ACCCATGATT TGGACACTTT GTCGGGCCCA TTTACTTATA CTGCAAAGCG TCCTTCAGAT 
ATCAAATTCA AGCCTCTAAA TAAGACCAAG GAGTATACAG CCTGTGAACT GATGAACATA 
TACAAGACTG ACAATCACCT GAAACATTAT TTACATATCA TTGAAAACAA ACCCCTGTAT 
CCAGTTATCT ATGATAGCAA TGGTGTCGTC CTTTCAATGC CTCCCATCAT CAATGGGGAT 
CATTCCAGAA TAACAGTAAA TACTAGAAAT ATTTTTATTG AATGCACGGG AACTGACTTT 
ACTAAGGCAA AAATAGTTCT TGATATTATT GTCACCATGT TCAGTGAATA TTGTGAGAAT 
CAATTTACGG TCGAAGCTGC TGAAGTGGTT TTTCCTAATG GAAAATCACA TACCTTTCCA 
GAATTAGCTT ACCGAAAGGA GATGGTGAGA GCTGACCTAA TTAACAAAAA AGTTGGAATC 
AGAGAAACTC CAGAAAATCT TGCCAAACTT CTGACCAGGA TGTATTTAAA ATCAGAAGTC 
ATAGGTGATG GGAATCAGAT TGAGATTGAA ATCCCTCCAA CCAGAGCTGA CATTATCCAT 
GCATGTGATA TTGTAGAAGA TGCAGCTATT GCTTATGGAT ATAACAACAT TCAGATGACT 
CTCCCGAAAA CTTACACCAT AGCTAATCAA TTTCCTCTTA ATAAGCTCAC TGAACTTCTC 
CGACATGACA TGGCAGCCGC TGGCTTCACT GAAGCACTTA CCTTTGCCCT GTGCTCCCAA 
GAAGATATTG CTGATAAACT AGGTGTGGAT ATCTCTGCAA CAAAGGCAGT CCACATAAGT 
AATCCTAAAA CAGCTGAATT TCAGGTGGCA CGCACTACCC TTCTTCCTGG CCTCCTGAAG 
ACCATAGCAG CAAATCGTAA GATGCCCCTT CCACTGAAAC TGTTTGAAAT CTCTGACATT 
GTAATAAAAG ATTCTAATAC AGATGTAGGT GCAAAAAACT ACAGACATCT CTGTGCTGTT 
TATTACAACA AGAATCCTGG GTTTGAGATC ATTCATGGGC TGCTGGACAG AATTATGCAG 
TTGCTCGATG TGCCTCCTGG TGAAGACAAG GGGGGATATG TGATCAAAGC ATCAGAAGGG 
CCTGCTTTCT TCCCCGGGCG ATGTGCAGAG ATCTTTGCCA GGGGTCAAAG CGTCGGGAAG 
CTTGGGGTCC TTCATCCTGA CGTTATCACC AAATTTGAGC TGACCATGCC CTGCTCCTCC 
CTAGAAATCA ATGTTGGACC CTTTTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005678.3
CDS	283..2052
Translation	MPTVSVKRDLLFQALGRTYTDEEFDELCFEFGLELDEITSEKEIISKEQGNVKAAGASDVVLYKIDVPANRYDLLCLEGLVRGLQVFKERIKAPVYKRVMPDGKIQKLIITEETAKIRPFAVAAVLRNIKFTKDRYDSFIELQEKLHQNICRKRALVAIGTHDLDTLSGPFTYTAKRPSDIKFKPLNKTKEYTACELMNIYKTDNHLKHYLHIIENKPLYPVIYDSNGVVLSMPPIINGDHSRITVNTRNIFIECTGTDFTKAKIVLDIIVTMFSEYCENQFTVEAAEVVFPNGKSHTFPELAYRKEMVRADLINKKVGIRETPENLAKLLTRMYLKSEVIGDGNQIEIEIPPTRADIIHACDIVEDAAIAYGYNNIQMTLPKTYTIANQFPLNKLTELLRHDMAAAGFTEALTFALCSQEDIADKLGVDISATKAVHISNPKTAEFQVARTTLLPGLLKTIAANRKMPLPLKLFEISDIVIKDSNTDVGAKNYRHLCAVYYNKNPGFEIIHGLLDRIMQLLDVPPGEDKGGYVIKASEGPAFFPGRCAEIFARGQSVGKLGVLHPDVITKFELTMPCSSLEINVGPFL

Target ORF information:

RefSeq Version	NM_005687.4
Organism	Homo sapiens(human)
Definition	Homo sapiens phenylalanyl-tRNA synthetase subunit beta (FARSB), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005687.4

ORF Insert Sequence:

ATGCCGACTG TCAGCGTGAA GCGTGATCTG CTCTTCCAAG CCCTGGGCCG CACCTACACT 
GACGAAGAAT TTGATGAACT ATGTTTTGAA TTTGGTCTGG AGCTTGATGA AATTACATCT 
GAGAAGGAAA TAATAAGTAA AGAACAAGGT AATGTAAAGG CAGCAGGAGC CTCTGATGTT 
GTTCTTTACA AAATTGACGT CCCTGCCAAT AGATATGATC TCCTGTGTCT GGAAGGATTG 
GTTCGAGGAC TTCAGGTCTT CAAAGAAAGG ATAAAGGCTC CAGTGTATAA ACGGGTAATG 
CCTGATGGAA AAATCCAGAA ATTGATTATC ACAGAAGAGA CAGCTAAGAT ACGTCCTTTT 
GCGGTAGCAG CAGTTCTCCG TAATATAAAG TTTACTAAAG ATCGATATGA CAGCTTCATT 
GAACTTCAGG AGAAATTACA TCAGAATATT TGCAGGAAAA GAGCACTGGT TGCCATTGGT 
ACCCATGATT TGGACACTTT GTCGGGCCCA TTTACTTATA CTGCAAAGCG TCCTTCAGAT 
ATCAAATTCA AGCCTCTAAA TAAGACCAAG GAGTATACAG CCTGTGAACT GATGAACATA 
TACAAGACTG ACAATCACCT GAAACATTAT TTACATATCA TTGAAAACAA ACCCCTGTAT 
CCAGTTATCT ATGATAGCAA TGGTGTCGTC CTTTCAATGC CTCCCATCAT CAATGGGGAT 
CATTCCAGAA TAACAGTAAA TACTAGAAAT ATTTTTATTG AATGCACGGG AACTGACTTT 
ACTAAGGCAA AAATAGTTCT TGATATTATT GTCACCATGT TCAGTGAATA TTGTGAGAAT 
CAATTTACGG TCGAAGCTGC TGAAGTGGTT TTTCCTAATG GAAAATCACA TACCTTTCCA 
GAATTAGCTT ACCGAAAGGA GATGGTGAGA GCTGACCTAA TTAACAAAAA AGTTGGAATC 
AGAGAAACTC CAGAAAATCT TGCCAAACTT CTGACCAGGA TGTATTTAAA ATCAGAAGTC 
ATAGGTGATG GGAATCAGAT TGAGATTGAA ATCCCTCCAA CCAGAGCTGA CATTATCCAT 
GCATGTGATA TTGTAGAAGA TGCAGCTATT GCTTATGGAT ATAACAACAT TCAGATGACT 
CTCCCGAAAA CTTACACCAT AGCTAATCAA TTTCCTCTTA ATAAGCTCAC TGAACTTCTC 
CGACATGACA TGGCAGCCGC TGGCTTCACT GAAGCACTTA CCTTTGCCCT GTGCTCCCAA 
GAAGATATTG CTGATAAACT AGGTGTGGAT ATCTCTGCAA CAAAGGCAGT CCACATAAGT 
AATCCTAAAA CAGCTGAATT TCAGGTGGCA CGCACTACCC TTCTTCCTGG CCTCCTGAAG 
ACCATAGCAG CAAATCGTAA GATGCCCCTT CCACTGAAAC TGTTTGAAAT CTCTGACATT 
GTAATAAAAG ATTCTAATAC AGATGTAGGT GCAAAAAACT ACAGACATCT CTGTGCTGTT 
TATTACAACA AGAATCCTGG GTTTGAGATC ATTCATGGGC TGCTGGACAG AATTATGCAG 
TTGCTCGATG TGCCTCCTGG TGAAGACAAG GGGGGATATG TGATCAAAGC ATCAGAAGGG 
CCTGCTTTCT TCCCCGGGCG ATGTGCAGAG ATCTTTGCCA GGGGTCAAAG CGTCGGGAAG 
CTTGGGGTCC TTCATCCTGA CGTTATCACC AAATTTGAGC TGACCATGCC CTGCTCCTCC 
CTAGAAATCA ATGTTGGACC CTTTTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FARSB ( NM_005687.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_005678.3 Homo sapiens phenylalanyl-tRNA synthetase subunit beta (FARSB), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu05107
Clone ID Related Accession (Same CDS sequence)	NM_005687.4 , NM_005687.5
Accession Version	NM_005687.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1770bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-10
Organism	Homo sapiens(human)
Product	phenylalanine--tRNA ligase beta subunit
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BG721840.1, AF042346.1 and AC097461.3. On May 17, 2019 this sequence version replaced NM_005687.4. Transcript Variant: This variant (1) represents the shorter transcript and encodes the functional protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.157152.1, SRR1660803.72872.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000281828.8/ ENSP00000281828.6 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCCGACTG TCAGCGTGAA GCGTGATCTG CTCTTCCAAG CCCTGGGCCG CACCTACACT 
GACGAAGAAT TTGATGAACT ATGTTTTGAA TTTGGTCTGG AGCTTGATGA AATTACATCT 
GAGAAGGAAA TAATAAGTAA AGAACAAGGT AATGTAAAGG CAGCAGGAGC CTCTGATGTT 
GTTCTTTACA AAATTGACGT CCCTGCCAAT AGATATGATC TCCTGTGTCT GGAAGGATTG 
GTTCGAGGAC TTCAGGTCTT CAAAGAAAGG ATAAAGGCTC CAGTGTATAA ACGGGTAATG 
CCTGATGGAA AAATCCAGAA ATTGATTATC ACAGAAGAGA CAGCTAAGAT ACGTCCTTTT 
GCGGTAGCAG CAGTTCTCCG TAATATAAAG TTTACTAAAG ATCGATATGA CAGCTTCATT 
GAACTTCAGG AGAAATTACA TCAGAATATT TGCAGGAAAA GAGCACTGGT TGCCATTGGT 
ACCCATGATT TGGACACTTT GTCGGGCCCA TTTACTTATA CTGCAAAGCG TCCTTCAGAT 
ATCAAATTCA AGCCTCTAAA TAAGACCAAG GAGTATACAG CCTGTGAACT GATGAACATA 
TACAAGACTG ACAATCACCT GAAACATTAT TTACATATCA TTGAAAACAA ACCCCTGTAT 
CCAGTTATCT ATGATAGCAA TGGTGTCGTC CTTTCAATGC CTCCCATCAT CAATGGGGAT 
CATTCCAGAA TAACAGTAAA TACTAGAAAT ATTTTTATTG AATGCACGGG AACTGACTTT 
ACTAAGGCAA AAATAGTTCT TGATATTATT GTCACCATGT TCAGTGAATA TTGTGAGAAT 
CAATTTACGG TCGAAGCTGC TGAAGTGGTT TTTCCTAATG GAAAATCACA TACCTTTCCA 
GAATTAGCTT ACCGAAAGGA GATGGTGAGA GCTGACCTAA TTAACAAAAA AGTTGGAATC 
AGAGAAACTC CAGAAAATCT TGCCAAACTT CTGACCAGGA TGTATTTAAA ATCAGAAGTC 
ATAGGTGATG GGAATCAGAT TGAGATTGAA ATCCCTCCAA CCAGAGCTGA CATTATCCAT 
GCATGTGATA TTGTAGAAGA TGCAGCTATT GCTTATGGAT ATAACAACAT TCAGATGACT 
CTCCCGAAAA CTTACACCAT AGCTAATCAA TTTCCTCTTA ATAAGCTCAC TGAACTTCTC 
CGACATGACA TGGCAGCCGC TGGCTTCACT GAAGCACTTA CCTTTGCCCT GTGCTCCCAA 
GAAGATATTG CTGATAAACT AGGTGTGGAT ATCTCTGCAA CAAAGGCAGT CCACATAAGT 
AATCCTAAAA CAGCTGAATT TCAGGTGGCA CGCACTACCC TTCTTCCTGG CCTCCTGAAG 
ACCATAGCAG CAAATCGTAA GATGCCCCTT CCACTGAAAC TGTTTGAAAT CTCTGACATT 
GTAATAAAAG ATTCTAATAC AGATGTAGGT GCAAAAAACT ACAGACATCT CTGTGCTGTT 
TATTACAACA AGAATCCTGG GTTTGAGATC ATTCATGGGC TGCTGGACAG AATTATGCAG 
TTGCTCGATG TGCCTCCTGG TGAAGACAAG GGGGGATATG TGATCAAAGC ATCAGAAGGG 
CCTGCTTTCT TCCCCGGGCG ATGTGCAGAG ATCTTTGCCA GGGGTCAAAG CGTCGGGAAG 
CTTGGGGTCC TTCATCCTGA CGTTATCACC AAATTTGAGC TGACCATGCC CTGCTCCTCC 
CTAGAAATCA ATGTTGGACC CTTTTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005678.3
CDS	20..1789
Translation	MPTVSVKRDLLFQALGRTYTDEEFDELCFEFGLELDEITSEKEIISKEQGNVKAAGASDVVLYKIDVPANRYDLLCLEGLVRGLQVFKERIKAPVYKRVMPDGKIQKLIITEETAKIRPFAVAAVLRNIKFTKDRYDSFIELQEKLHQNICRKRALVAIGTHDLDTLSGPFTYTAKRPSDIKFKPLNKTKEYTACELMNIYKTDNHLKHYLHIIENKPLYPVIYDSNGVVLSMPPIINGDHSRITVNTRNIFIECTGTDFTKAKIVLDIIVTMFSEYCENQFTVEAAEVVFPNGKSHTFPELAYRKEMVRADLINKKVGIRETPENLAKLLTRMYLKSEVIGDGNQIEIEIPPTRADIIHACDIVEDAAIAYGYNNIQMTLPKTYTIANQFPLNKLTELLRHDMAAAGFTEALTFALCSQEDIADKLGVDISATKAVHISNPKTAEFQVARTTLLPGLLKTIAANRKMPLPLKLFEISDIVIKDSNTDVGAKNYRHLCAVYYNKNPGFEIIHGLLDRIMQLLDVPPGEDKGGYVIKASEGPAFFPGRCAEIFARGQSVGKLGVLHPDVITKFELTMPCSSLEINVGPFL

Target ORF information:

RefSeq Version	NM_005687.5
Organism	Homo sapiens(human)
Definition	Homo sapiens phenylalanyl-tRNA synthetase subunit beta (FARSB), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005687.5

ORF Insert Sequence:

ATGCCGACTG TCAGCGTGAA GCGTGATCTG CTCTTCCAAG CCCTGGGCCG CACCTACACT 
GACGAAGAAT TTGATGAACT ATGTTTTGAA TTTGGTCTGG AGCTTGATGA AATTACATCT 
GAGAAGGAAA TAATAAGTAA AGAACAAGGT AATGTAAAGG CAGCAGGAGC CTCTGATGTT 
GTTCTTTACA AAATTGACGT CCCTGCCAAT AGATATGATC TCCTGTGTCT GGAAGGATTG 
GTTCGAGGAC TTCAGGTCTT CAAAGAAAGG ATAAAGGCTC CAGTGTATAA ACGGGTAATG 
CCTGATGGAA AAATCCAGAA ATTGATTATC ACAGAAGAGA CAGCTAAGAT ACGTCCTTTT 
GCGGTAGCAG CAGTTCTCCG TAATATAAAG TTTACTAAAG ATCGATATGA CAGCTTCATT 
GAACTTCAGG AGAAATTACA TCAGAATATT TGCAGGAAAA GAGCACTGGT TGCCATTGGT 
ACCCATGATT TGGACACTTT GTCGGGCCCA TTTACTTATA CTGCAAAGCG TCCTTCAGAT 
ATCAAATTCA AGCCTCTAAA TAAGACCAAG GAGTATACAG CCTGTGAACT GATGAACATA 
TACAAGACTG ACAATCACCT GAAACATTAT TTACATATCA TTGAAAACAA ACCCCTGTAT 
CCAGTTATCT ATGATAGCAA TGGTGTCGTC CTTTCAATGC CTCCCATCAT CAATGGGGAT 
CATTCCAGAA TAACAGTAAA TACTAGAAAT ATTTTTATTG AATGCACGGG AACTGACTTT 
ACTAAGGCAA AAATAGTTCT TGATATTATT GTCACCATGT TCAGTGAATA TTGTGAGAAT 
CAATTTACGG TCGAAGCTGC TGAAGTGGTT TTTCCTAATG GAAAATCACA TACCTTTCCA 
GAATTAGCTT ACCGAAAGGA GATGGTGAGA GCTGACCTAA TTAACAAAAA AGTTGGAATC 
AGAGAAACTC CAGAAAATCT TGCCAAACTT CTGACCAGGA TGTATTTAAA ATCAGAAGTC 
ATAGGTGATG GGAATCAGAT TGAGATTGAA ATCCCTCCAA CCAGAGCTGA CATTATCCAT 
GCATGTGATA TTGTAGAAGA TGCAGCTATT GCTTATGGAT ATAACAACAT TCAGATGACT 
CTCCCGAAAA CTTACACCAT AGCTAATCAA TTTCCTCTTA ATAAGCTCAC TGAACTTCTC 
CGACATGACA TGGCAGCCGC TGGCTTCACT GAAGCACTTA CCTTTGCCCT GTGCTCCCAA 
GAAGATATTG CTGATAAACT AGGTGTGGAT ATCTCTGCAA CAAAGGCAGT CCACATAAGT 
AATCCTAAAA CAGCTGAATT TCAGGTGGCA CGCACTACCC TTCTTCCTGG CCTCCTGAAG 
ACCATAGCAG CAAATCGTAA GATGCCCCTT CCACTGAAAC TGTTTGAAAT CTCTGACATT 
GTAATAAAAG ATTCTAATAC AGATGTAGGT GCAAAAAACT ACAGACATCT CTGTGCTGTT 
TATTACAACA AGAATCCTGG GTTTGAGATC ATTCATGGGC TGCTGGACAG AATTATGCAG 
TTGCTCGATG TGCCTCCTGG TGAAGACAAG GGGGGATATG TGATCAAAGC ATCAGAAGGG 
CCTGCTTTCT TCCCCGGGCG ATGTGCAGAG ATCTTTGCCA GGGGTCAAAG CGTCGGGAAG 
CTTGGGGTCC TTCATCCTGA CGTTATCACC AAATTTGAGC TGACCATGCC CTGCTCCTCC 
CTAGAAATCA ATGTTGGACC CTTTTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FARSB ( XM_017003110.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016858599.1 Homo sapiens phenylalanyl-tRNA synthetase subunit beta (FARSB), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu35721
Clone ID Related Accession (Same CDS sequence)	XM_017003110.2 , XM_006712169.2 , XM_011510466.2 , XM_017003110.1 , XM_024452492.1
Accession Version	XM_017003110.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1473bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	phenylalanine--tRNA ligase beta subunit isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017003110.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGATG GAAAAATCCA GAAATTGATT ATCACAGAAG AGACAGCTAA GATACGTCCT 
TTTGCGGTAG CAGCAGTTCT CCGTAATATA AAGTTTACTA AAGATCGATA TGACAGCTTC 
ATTGAACTTC AGGAGAAATT ACATCAGAAT ATTTGCAGGA AAAGAGCACT GGTTGCCATT 
GGTACCCATG ATTTGGACAC TTTGTCGGGC CCATTTACTT ATACTGCAAA GCGTCCTTCA 
GATATCAAAT TCAAGCCTCT AAATAAGACC AAGGAGTATA CAGCCTGTGA ACTGATGAAC 
ATATACAAGA CTGACAATCA CCTGAAACAT TATTTACATA TCATTGAAAA CAAACCCCTG 
TATCCAGTTA TCTATGATAG CAATGGTGTC GTCCTTTCAA TGCCTCCCAT CATCAATGGG 
GATCATTCCA GAATAACAGT AAATACTAGA AATATTTTTA TTGAATGCAC GGGAACTGAC 
TTTACTAAGG CAAAAATAGT TCTTGATATT ATTGTCACCA TGTTCAGTGA ATATTGTGAG 
AATCAATTTA CGGTCGAAGC TGCTGAAGTG GTTTTTCCTA ATGGAAAATC ACATACCTTT 
CCAGAATTAG CTTACCGAAA GGAGATGGTG AGAGCTGACC TAATTAACAA AAAAGTTGGA 
ATCAGAGAAA CTCCAGAAAA TCTTGCCAAA CTTCTGACCA GGATGTATTT AAAATCAGAA 
GTCATAGGTG ATGGGAATCA GATTGAGATT GAAATCCCTC CAACCAGAGC TGACATTATC 
CATGCATGTG ATATTGTAGA AGATGCAGCT ATTGCTTATG GATATAACAA CATTCAGATG 
ACTCTCCCGA AAACTTACAC CATAGCTAAT CAATTTCCTC TTAATAAGCT CACTGAACTT 
CTCCGACATG ACATGGCAGC CGCTGGCTTC ACTGAAGCAC TTACCTTTGC CCTGTGCTCC 
CAAGAAGATA TTGCTGATAA ACTAGGTGTG GATATCTCTG CAACAAAGGC AGTCCACATA 
AGTAATCCTA AAACAGCTGA ATTTCAGGTG GCACGCACTA CCCTTCTTCC TGGCCTCCTG 
AAGACCATAG CAGCAAATCG TAAGATGCCC CTTCCACTGA AACTGTTTGA AATCTCTGAC 
ATTGTAATAA AAGATTCTAA TACAGATGTA GGTGCAAAAA ACTACAGACA TCTCTGTGCT 
GTTTATTACA ACAAGAATCC TGGGTTTGAG ATCATTCATG GGCTGCTGGA CAGAATTATG 
CAGTTGCTCG ATGTGCCTCC TGGTGAAGAC AAGGGGGGAT ATGTGATCAA AGCATCAGAA 
GGGCCTGCTT TCTTCCCCGG GCGATGTGCA GAGATCTTTG CCAGGGGTCA AAGCGTCGGG 
AAGCTTGGGG TCCTTCATCC TGACGTTATC ACCAAATTTG AGCTGACCAT GCCCTGCTCC 
TCCCTAGAAA TCAATGTTGG ACCCTTTTTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016858599.1
CDS	2747..4219
Translation	MPDGKIQKLIITEETAKIRPFAVAAVLRNIKFTKDRYDSFIELQEKLHQNICRKRALVAIGTHDLDTLSGPFTYTAKRPSDIKFKPLNKTKEYTACELMNIYKTDNHLKHYLHIIENKPLYPVIYDSNGVVLSMPPIINGDHSRITVNTRNIFIECTGTDFTKAKIVLDIIVTMFSEYCENQFTVEAAEVVFPNGKSHTFPELAYRKEMVRADLINKKVGIRETPENLAKLLTRMYLKSEVIGDGNQIEIEIPPTRADIIHACDIVEDAAIAYGYNNIQMTLPKTYTIANQFPLNKLTELLRHDMAAAGFTEALTFALCSQEDIADKLGVDISATKAVHISNPKTAEFQVARTTLLPGLLKTIAANRKMPLPLKLFEISDIVIKDSNTDVGAKNYRHLCAVYYNKNPGFEIIHGLLDRIMQLLDVPPGEDKGGYVIKASEGPAFFPGRCAEIFARGQSVGKLGVLHPDVITKFELTMPCSSLEINVGPFL

Target ORF information:

RefSeq Version	XM_017003110.2
Organism	Homo sapiens(human)
Definition	Homo sapiens phenylalanyl-tRNA synthetase subunit beta (FARSB), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017003110.2

ORF Insert Sequence:

ATGCCTGATG GAAAAATCCA GAAATTGATT ATCACAGAAG AGACAGCTAA GATACGTCCT 
TTTGCGGTAG CAGCAGTTCT CCGTAATATA AAGTTTACTA AAGATCGATA TGACAGCTTC 
ATTGAACTTC AGGAGAAATT ACATCAGAAT ATTTGCAGGA AAAGAGCACT GGTTGCCATT 
GGTACCCATG ATTTGGACAC TTTGTCGGGC CCATTTACTT ATACTGCAAA GCGTCCTTCA 
GATATCAAAT TCAAGCCTCT AAATAAGACC AAGGAGTATA CAGCCTGTGA ACTGATGAAC 
ATATACAAGA CTGACAATCA CCTGAAACAT TATTTACATA TCATTGAAAA CAAACCCCTG 
TATCCAGTTA TCTATGATAG CAATGGTGTC GTCCTTTCAA TGCCTCCCAT CATCAATGGG 
GATCATTCCA GAATAACAGT AAATACTAGA AATATTTTTA TTGAATGCAC GGGAACTGAC 
TTTACTAAGG CAAAAATAGT TCTTGATATT ATTGTCACCA TGTTCAGTGA ATATTGTGAG 
AATCAATTTA CGGTCGAAGC TGCTGAAGTG GTTTTTCCTA ATGGAAAATC ACATACCTTT 
CCAGAATTAG CTTACCGAAA GGAGATGGTG AGAGCTGACC TAATTAACAA AAAAGTTGGA 
ATCAGAGAAA CTCCAGAAAA TCTTGCCAAA CTTCTGACCA GGATGTATTT AAAATCAGAA 
GTCATAGGTG ATGGGAATCA GATTGAGATT GAAATCCCTC CAACCAGAGC TGACATTATC 
CATGCATGTG ATATTGTAGA AGATGCAGCT ATTGCTTATG GATATAACAA CATTCAGATG 
ACTCTCCCGA AAACTTACAC CATAGCTAAT CAATTTCCTC TTAATAAGCT CACTGAACTT 
CTCCGACATG ACATGGCAGC CGCTGGCTTC ACTGAAGCAC TTACCTTTGC CCTGTGCTCC 
CAAGAAGATA TTGCTGATAA ACTAGGTGTG GATATCTCTG CAACAAAGGC AGTCCACATA 
AGTAATCCTA AAACAGCTGA ATTTCAGGTG GCACGCACTA CCCTTCTTCC TGGCCTCCTG 
AAGACCATAG CAGCAAATCG TAAGATGCCC CTTCCACTGA AACTGTTTGA AATCTCTGAC 
ATTGTAATAA AAGATTCTAA TACAGATGTA GGTGCAAAAA ACTACAGACA TCTCTGTGCT 
GTTTATTACA ACAAGAATCC TGGGTTTGAG ATCATTCATG GGCTGCTGGA CAGAATTATG 
CAGTTGCTCG ATGTGCCTCC TGGTGAAGAC AAGGGGGGAT ATGTGATCAA AGCATCAGAA 
GGGCCTGCTT TCTTCCCCGG GCGATGTGCA GAGATCTTTG CCAGGGGTCA AAGCGTCGGG 
AAGCTTGGGG TCCTTCATCC TGACGTTATC ACCAAATTTG AGCTGACCAT GCCCTGCTCC 
TCCCTAGAAA TCAATGTTGG ACCCTTTTTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FARSB ( XM_006712169.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_006712232.1 Homo sapiens phenylalanyl-tRNA synthetase subunit beta (FARSB), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu35721
Clone ID Related Accession (Same CDS sequence)	XM_017003110.2 , XM_006712169.2 , XM_011510466.2 , XM_017003110.1 , XM_024452492.1
Accession Version	XM_006712169.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1473bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	phenylalanine--tRNA ligase beta subunit isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006712169.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGATG GAAAAATCCA GAAATTGATT ATCACAGAAG AGACAGCTAA GATACGTCCT 
TTTGCGGTAG CAGCAGTTCT CCGTAATATA AAGTTTACTA AAGATCGATA TGACAGCTTC 
ATTGAACTTC AGGAGAAATT ACATCAGAAT ATTTGCAGGA AAAGAGCACT GGTTGCCATT 
GGTACCCATG ATTTGGACAC TTTGTCGGGC CCATTTACTT ATACTGCAAA GCGTCCTTCA 
GATATCAAAT TCAAGCCTCT AAATAAGACC AAGGAGTATA CAGCCTGTGA ACTGATGAAC 
ATATACAAGA CTGACAATCA CCTGAAACAT TATTTACATA TCATTGAAAA CAAACCCCTG 
TATCCAGTTA TCTATGATAG CAATGGTGTC GTCCTTTCAA TGCCTCCCAT CATCAATGGG 
GATCATTCCA GAATAACAGT AAATACTAGA AATATTTTTA TTGAATGCAC GGGAACTGAC 
TTTACTAAGG CAAAAATAGT TCTTGATATT ATTGTCACCA TGTTCAGTGA ATATTGTGAG 
AATCAATTTA CGGTCGAAGC TGCTGAAGTG GTTTTTCCTA ATGGAAAATC ACATACCTTT 
CCAGAATTAG CTTACCGAAA GGAGATGGTG AGAGCTGACC TAATTAACAA AAAAGTTGGA 
ATCAGAGAAA CTCCAGAAAA TCTTGCCAAA CTTCTGACCA GGATGTATTT AAAATCAGAA 
GTCATAGGTG ATGGGAATCA GATTGAGATT GAAATCCCTC CAACCAGAGC TGACATTATC 
CATGCATGTG ATATTGTAGA AGATGCAGCT ATTGCTTATG GATATAACAA CATTCAGATG 
ACTCTCCCGA AAACTTACAC CATAGCTAAT CAATTTCCTC TTAATAAGCT CACTGAACTT 
CTCCGACATG ACATGGCAGC CGCTGGCTTC ACTGAAGCAC TTACCTTTGC CCTGTGCTCC 
CAAGAAGATA TTGCTGATAA ACTAGGTGTG GATATCTCTG CAACAAAGGC AGTCCACATA 
AGTAATCCTA AAACAGCTGA ATTTCAGGTG GCACGCACTA CCCTTCTTCC TGGCCTCCTG 
AAGACCATAG CAGCAAATCG TAAGATGCCC CTTCCACTGA AACTGTTTGA AATCTCTGAC 
ATTGTAATAA AAGATTCTAA TACAGATGTA GGTGCAAAAA ACTACAGACA TCTCTGTGCT 
GTTTATTACA ACAAGAATCC TGGGTTTGAG ATCATTCATG GGCTGCTGGA CAGAATTATG 
CAGTTGCTCG ATGTGCCTCC TGGTGAAGAC AAGGGGGGAT ATGTGATCAA AGCATCAGAA 
GGGCCTGCTT TCTTCCCCGG GCGATGTGCA GAGATCTTTG CCAGGGGTCA AAGCGTCGGG 
AAGCTTGGGG TCCTTCATCC TGACGTTATC ACCAAATTTG AGCTGACCAT GCCCTGCTCC 
TCCCTAGAAA TCAATGTTGG ACCCTTTTTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006712232.1
CDS	482..1954
Translation	MPDGKIQKLIITEETAKIRPFAVAAVLRNIKFTKDRYDSFIELQEKLHQNICRKRALVAIGTHDLDTLSGPFTYTAKRPSDIKFKPLNKTKEYTACELMNIYKTDNHLKHYLHIIENKPLYPVIYDSNGVVLSMPPIINGDHSRITVNTRNIFIECTGTDFTKAKIVLDIIVTMFSEYCENQFTVEAAEVVFPNGKSHTFPELAYRKEMVRADLINKKVGIRETPENLAKLLTRMYLKSEVIGDGNQIEIEIPPTRADIIHACDIVEDAAIAYGYNNIQMTLPKTYTIANQFPLNKLTELLRHDMAAAGFTEALTFALCSQEDIADKLGVDISATKAVHISNPKTAEFQVARTTLLPGLLKTIAANRKMPLPLKLFEISDIVIKDSNTDVGAKNYRHLCAVYYNKNPGFEIIHGLLDRIMQLLDVPPGEDKGGYVIKASEGPAFFPGRCAEIFARGQSVGKLGVLHPDVITKFELTMPCSSLEINVGPFL

Target ORF information:

RefSeq Version	XM_006712169.2
Organism	Homo sapiens(human)
Definition	Homo sapiens phenylalanyl-tRNA synthetase subunit beta (FARSB), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006712169.2

ORF Insert Sequence:

ATGCCTGATG GAAAAATCCA GAAATTGATT ATCACAGAAG AGACAGCTAA GATACGTCCT 
TTTGCGGTAG CAGCAGTTCT CCGTAATATA AAGTTTACTA AAGATCGATA TGACAGCTTC 
ATTGAACTTC AGGAGAAATT ACATCAGAAT ATTTGCAGGA AAAGAGCACT GGTTGCCATT 
GGTACCCATG ATTTGGACAC TTTGTCGGGC CCATTTACTT ATACTGCAAA GCGTCCTTCA 
GATATCAAAT TCAAGCCTCT AAATAAGACC AAGGAGTATA CAGCCTGTGA ACTGATGAAC 
ATATACAAGA CTGACAATCA CCTGAAACAT TATTTACATA TCATTGAAAA CAAACCCCTG 
TATCCAGTTA TCTATGATAG CAATGGTGTC GTCCTTTCAA TGCCTCCCAT CATCAATGGG 
GATCATTCCA GAATAACAGT AAATACTAGA AATATTTTTA TTGAATGCAC GGGAACTGAC 
TTTACTAAGG CAAAAATAGT TCTTGATATT ATTGTCACCA TGTTCAGTGA ATATTGTGAG 
AATCAATTTA CGGTCGAAGC TGCTGAAGTG GTTTTTCCTA ATGGAAAATC ACATACCTTT 
CCAGAATTAG CTTACCGAAA GGAGATGGTG AGAGCTGACC TAATTAACAA AAAAGTTGGA 
ATCAGAGAAA CTCCAGAAAA TCTTGCCAAA CTTCTGACCA GGATGTATTT AAAATCAGAA 
GTCATAGGTG ATGGGAATCA GATTGAGATT GAAATCCCTC CAACCAGAGC TGACATTATC 
CATGCATGTG ATATTGTAGA AGATGCAGCT ATTGCTTATG GATATAACAA CATTCAGATG 
ACTCTCCCGA AAACTTACAC CATAGCTAAT CAATTTCCTC TTAATAAGCT CACTGAACTT 
CTCCGACATG ACATGGCAGC CGCTGGCTTC ACTGAAGCAC TTACCTTTGC CCTGTGCTCC 
CAAGAAGATA TTGCTGATAA ACTAGGTGTG GATATCTCTG CAACAAAGGC AGTCCACATA 
AGTAATCCTA AAACAGCTGA ATTTCAGGTG GCACGCACTA CCCTTCTTCC TGGCCTCCTG 
AAGACCATAG CAGCAAATCG TAAGATGCCC CTTCCACTGA AACTGTTTGA AATCTCTGAC 
ATTGTAATAA AAGATTCTAA TACAGATGTA GGTGCAAAAA ACTACAGACA TCTCTGTGCT 
GTTTATTACA ACAAGAATCC TGGGTTTGAG ATCATTCATG GGCTGCTGGA CAGAATTATG 
CAGTTGCTCG ATGTGCCTCC TGGTGAAGAC AAGGGGGGAT ATGTGATCAA AGCATCAGAA 
GGGCCTGCTT TCTTCCCCGG GCGATGTGCA GAGATCTTTG CCAGGGGTCA AAGCGTCGGG 
AAGCTTGGGG TCCTTCATCC TGACGTTATC ACCAAATTTG AGCTGACCAT GCCCTGCTCC 
TCCCTAGAAA TCAATGTTGG ACCCTTTTTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FARSB ( XM_011510466.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011508768.1 Homo sapiens phenylalanyl-tRNA synthetase subunit beta (FARSB), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHu35721
Clone ID Related Accession (Same CDS sequence)	XM_017003110.2 , XM_006712169.2 , XM_011510466.2 , XM_017003110.1 , XM_024452492.1
Accession Version	XM_011510466.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1473bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	phenylalanine--tRNA ligase beta subunit isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011510466.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGATG GAAAAATCCA GAAATTGATT ATCACAGAAG AGACAGCTAA GATACGTCCT 
TTTGCGGTAG CAGCAGTTCT CCGTAATATA AAGTTTACTA AAGATCGATA TGACAGCTTC 
ATTGAACTTC AGGAGAAATT ACATCAGAAT ATTTGCAGGA AAAGAGCACT GGTTGCCATT 
GGTACCCATG ATTTGGACAC TTTGTCGGGC CCATTTACTT ATACTGCAAA GCGTCCTTCA 
GATATCAAAT TCAAGCCTCT AAATAAGACC AAGGAGTATA CAGCCTGTGA ACTGATGAAC 
ATATACAAGA CTGACAATCA CCTGAAACAT TATTTACATA TCATTGAAAA CAAACCCCTG 
TATCCAGTTA TCTATGATAG CAATGGTGTC GTCCTTTCAA TGCCTCCCAT CATCAATGGG 
GATCATTCCA GAATAACAGT AAATACTAGA AATATTTTTA TTGAATGCAC GGGAACTGAC 
TTTACTAAGG CAAAAATAGT TCTTGATATT ATTGTCACCA TGTTCAGTGA ATATTGTGAG 
AATCAATTTA CGGTCGAAGC TGCTGAAGTG GTTTTTCCTA ATGGAAAATC ACATACCTTT 
CCAGAATTAG CTTACCGAAA GGAGATGGTG AGAGCTGACC TAATTAACAA AAAAGTTGGA 
ATCAGAGAAA CTCCAGAAAA TCTTGCCAAA CTTCTGACCA GGATGTATTT AAAATCAGAA 
GTCATAGGTG ATGGGAATCA GATTGAGATT GAAATCCCTC CAACCAGAGC TGACATTATC 
CATGCATGTG ATATTGTAGA AGATGCAGCT ATTGCTTATG GATATAACAA CATTCAGATG 
ACTCTCCCGA AAACTTACAC CATAGCTAAT CAATTTCCTC TTAATAAGCT CACTGAACTT 
CTCCGACATG ACATGGCAGC CGCTGGCTTC ACTGAAGCAC TTACCTTTGC CCTGTGCTCC 
CAAGAAGATA TTGCTGATAA ACTAGGTGTG GATATCTCTG CAACAAAGGC AGTCCACATA 
AGTAATCCTA AAACAGCTGA ATTTCAGGTG GCACGCACTA CCCTTCTTCC TGGCCTCCTG 
AAGACCATAG CAGCAAATCG TAAGATGCCC CTTCCACTGA AACTGTTTGA AATCTCTGAC 
ATTGTAATAA AAGATTCTAA TACAGATGTA GGTGCAAAAA ACTACAGACA TCTCTGTGCT 
GTTTATTACA ACAAGAATCC TGGGTTTGAG ATCATTCATG GGCTGCTGGA CAGAATTATG 
CAGTTGCTCG ATGTGCCTCC TGGTGAAGAC AAGGGGGGAT ATGTGATCAA AGCATCAGAA 
GGGCCTGCTT TCTTCCCCGG GCGATGTGCA GAGATCTTTG CCAGGGGTCA AAGCGTCGGG 
AAGCTTGGGG TCCTTCATCC TGACGTTATC ACCAAATTTG AGCTGACCAT GCCCTGCTCC 
TCCCTAGAAA TCAATGTTGG ACCCTTTTTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011508768.1
CDS	354..1826
Translation	MPDGKIQKLIITEETAKIRPFAVAAVLRNIKFTKDRYDSFIELQEKLHQNICRKRALVAIGTHDLDTLSGPFTYTAKRPSDIKFKPLNKTKEYTACELMNIYKTDNHLKHYLHIIENKPLYPVIYDSNGVVLSMPPIINGDHSRITVNTRNIFIECTGTDFTKAKIVLDIIVTMFSEYCENQFTVEAAEVVFPNGKSHTFPELAYRKEMVRADLINKKVGIRETPENLAKLLTRMYLKSEVIGDGNQIEIEIPPTRADIIHACDIVEDAAIAYGYNNIQMTLPKTYTIANQFPLNKLTELLRHDMAAAGFTEALTFALCSQEDIADKLGVDISATKAVHISNPKTAEFQVARTTLLPGLLKTIAANRKMPLPLKLFEISDIVIKDSNTDVGAKNYRHLCAVYYNKNPGFEIIHGLLDRIMQLLDVPPGEDKGGYVIKASEGPAFFPGRCAEIFARGQSVGKLGVLHPDVITKFELTMPCSSLEINVGPFL

Target ORF information:

RefSeq Version	XM_011510466.2
Organism	Homo sapiens(human)
Definition	Homo sapiens phenylalanyl-tRNA synthetase subunit beta (FARSB), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011510466.2

ORF Insert Sequence:

ATGCCTGATG GAAAAATCCA GAAATTGATT ATCACAGAAG AGACAGCTAA GATACGTCCT 
TTTGCGGTAG CAGCAGTTCT CCGTAATATA AAGTTTACTA AAGATCGATA TGACAGCTTC 
ATTGAACTTC AGGAGAAATT ACATCAGAAT ATTTGCAGGA AAAGAGCACT GGTTGCCATT 
GGTACCCATG ATTTGGACAC TTTGTCGGGC CCATTTACTT ATACTGCAAA GCGTCCTTCA 
GATATCAAAT TCAAGCCTCT AAATAAGACC AAGGAGTATA CAGCCTGTGA ACTGATGAAC 
ATATACAAGA CTGACAATCA CCTGAAACAT TATTTACATA TCATTGAAAA CAAACCCCTG 
TATCCAGTTA TCTATGATAG CAATGGTGTC GTCCTTTCAA TGCCTCCCAT CATCAATGGG 
GATCATTCCA GAATAACAGT AAATACTAGA AATATTTTTA TTGAATGCAC GGGAACTGAC 
TTTACTAAGG CAAAAATAGT TCTTGATATT ATTGTCACCA TGTTCAGTGA ATATTGTGAG 
AATCAATTTA CGGTCGAAGC TGCTGAAGTG GTTTTTCCTA ATGGAAAATC ACATACCTTT 
CCAGAATTAG CTTACCGAAA GGAGATGGTG AGAGCTGACC TAATTAACAA AAAAGTTGGA 
ATCAGAGAAA CTCCAGAAAA TCTTGCCAAA CTTCTGACCA GGATGTATTT AAAATCAGAA 
GTCATAGGTG ATGGGAATCA GATTGAGATT GAAATCCCTC CAACCAGAGC TGACATTATC 
CATGCATGTG ATATTGTAGA AGATGCAGCT ATTGCTTATG GATATAACAA CATTCAGATG 
ACTCTCCCGA AAACTTACAC CATAGCTAAT CAATTTCCTC TTAATAAGCT CACTGAACTT 
CTCCGACATG ACATGGCAGC CGCTGGCTTC ACTGAAGCAC TTACCTTTGC CCTGTGCTCC 
CAAGAAGATA TTGCTGATAA ACTAGGTGTG GATATCTCTG CAACAAAGGC AGTCCACATA 
AGTAATCCTA AAACAGCTGA ATTTCAGGTG GCACGCACTA CCCTTCTTCC TGGCCTCCTG 
AAGACCATAG CAGCAAATCG TAAGATGCCC CTTCCACTGA AACTGTTTGA AATCTCTGAC 
ATTGTAATAA AAGATTCTAA TACAGATGTA GGTGCAAAAA ACTACAGACA TCTCTGTGCT 
GTTTATTACA ACAAGAATCC TGGGTTTGAG ATCATTCATG GGCTGCTGGA CAGAATTATG 
CAGTTGCTCG ATGTGCCTCC TGGTGAAGAC AAGGGGGGAT ATGTGATCAA AGCATCAGAA 
GGGCCTGCTT TCTTCCCCGG GCGATGTGCA GAGATCTTTG CCAGGGGTCA AAGCGTCGGG 
AAGCTTGGGG TCCTTCATCC TGACGTTATC ACCAAATTTG AGCTGACCAT GCCCTGCTCC 
TCCCTAGAAA TCAATGTTGG ACCCTTTTTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FARSB ( XM_017003110.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016858599.1 Homo sapiens phenylalanyl-tRNA synthetase beta subunit (FARSB), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu35721
Clone ID Related Accession (Same CDS sequence)	XM_017003110.2 , XM_006712169.2 , XM_011510466.2 , XM_017003110.1 , XM_024452492.1
Accession Version	XM_017003110.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1473bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	phenylalanine--tRNA ligase beta subunit isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGATG GAAAAATCCA GAAATTGATT ATCACAGAAG AGACAGCTAA GATACGTCCT 
TTTGCGGTAG CAGCAGTTCT CCGTAATATA AAGTTTACTA AAGATCGATA TGACAGCTTC 
ATTGAACTTC AGGAGAAATT ACATCAGAAT ATTTGCAGGA AAAGAGCACT GGTTGCCATT 
GGTACCCATG ATTTGGACAC TTTGTCGGGC CCATTTACTT ATACTGCAAA GCGTCCTTCA 
GATATCAAAT TCAAGCCTCT AAATAAGACC AAGGAGTATA CAGCCTGTGA ACTGATGAAC 
ATATACAAGA CTGACAATCA CCTGAAACAT TATTTACATA TCATTGAAAA CAAACCCCTG 
TATCCAGTTA TCTATGATAG CAATGGTGTC GTCCTTTCAA TGCCTCCCAT CATCAATGGG 
GATCATTCCA GAATAACAGT AAATACTAGA AATATTTTTA TTGAATGCAC GGGAACTGAC 
TTTACTAAGG CAAAAATAGT TCTTGATATT ATTGTCACCA TGTTCAGTGA ATATTGTGAG 
AATCAATTTA CGGTCGAAGC TGCTGAAGTG GTTTTTCCTA ATGGAAAATC ACATACCTTT 
CCAGAATTAG CTTACCGAAA GGAGATGGTG AGAGCTGACC TAATTAACAA AAAAGTTGGA 
ATCAGAGAAA CTCCAGAAAA TCTTGCCAAA CTTCTGACCA GGATGTATTT AAAATCAGAA 
GTCATAGGTG ATGGGAATCA GATTGAGATT GAAATCCCTC CAACCAGAGC TGACATTATC 
CATGCATGTG ATATTGTAGA AGATGCAGCT ATTGCTTATG GATATAACAA CATTCAGATG 
ACTCTCCCGA AAACTTACAC CATAGCTAAT CAATTTCCTC TTAATAAGCT CACTGAACTT 
CTCCGACATG ACATGGCAGC CGCTGGCTTC ACTGAAGCAC TTACCTTTGC CCTGTGCTCC 
CAAGAAGATA TTGCTGATAA ACTAGGTGTG GATATCTCTG CAACAAAGGC AGTCCACATA 
AGTAATCCTA AAACAGCTGA ATTTCAGGTG GCACGCACTA CCCTTCTTCC TGGCCTCCTG 
AAGACCATAG CAGCAAATCG TAAGATGCCC CTTCCACTGA AACTGTTTGA AATCTCTGAC 
ATTGTAATAA AAGATTCTAA TACAGATGTA GGTGCAAAAA ACTACAGACA TCTCTGTGCT 
GTTTATTACA ACAAGAATCC TGGGTTTGAG ATCATTCATG GGCTGCTGGA CAGAATTATG 
CAGTTGCTCG ATGTGCCTCC TGGTGAAGAC AAGGGGGGAT ATGTGATCAA AGCATCAGAA 
GGGCCTGCTT TCTTCCCCGG GCGATGTGCA GAGATCTTTG CCAGGGGTCA AAGCGTCGGG 
AAGCTTGGGG TCCTTCATCC TGACGTTATC ACCAAATTTG AGCTGACCAT GCCCTGCTCC 
TCCCTAGAAA TCAATGTTGG ACCCTTTTTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016858599.1
CDS	2755..4227
Translation	MPDGKIQKLIITEETAKIRPFAVAAVLRNIKFTKDRYDSFIELQEKLHQNICRKRALVAIGTHDLDTLSGPFTYTAKRPSDIKFKPLNKTKEYTACELMNIYKTDNHLKHYLHIIENKPLYPVIYDSNGVVLSMPPIINGDHSRITVNTRNIFIECTGTDFTKAKIVLDIIVTMFSEYCENQFTVEAAEVVFPNGKSHTFPELAYRKEMVRADLINKKVGIRETPENLAKLLTRMYLKSEVIGDGNQIEIEIPPTRADIIHACDIVEDAAIAYGYNNIQMTLPKTYTIANQFPLNKLTELLRHDMAAAGFTEALTFALCSQEDIADKLGVDISATKAVHISNPKTAEFQVARTTLLPGLLKTIAANRKMPLPLKLFEISDIVIKDSNTDVGAKNYRHLCAVYYNKNPGFEIIHGLLDRIMQLLDVPPGEDKGGYVIKASEGPAFFPGRCAEIFARGQSVGKLGVLHPDVITKFELTMPCSSLEINVGPFL

Target ORF information:

RefSeq Version	XM_017003110.1
Organism	Homo sapiens(human)
Definition	Homo sapiens phenylalanyl-tRNA synthetase beta subunit (FARSB), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017003110.1

ORF Insert Sequence:

ATGCCTGATG GAAAAATCCA GAAATTGATT ATCACAGAAG AGACAGCTAA GATACGTCCT 
TTTGCGGTAG CAGCAGTTCT CCGTAATATA AAGTTTACTA AAGATCGATA TGACAGCTTC 
ATTGAACTTC AGGAGAAATT ACATCAGAAT ATTTGCAGGA AAAGAGCACT GGTTGCCATT 
GGTACCCATG ATTTGGACAC TTTGTCGGGC CCATTTACTT ATACTGCAAA GCGTCCTTCA 
GATATCAAAT TCAAGCCTCT AAATAAGACC AAGGAGTATA CAGCCTGTGA ACTGATGAAC 
ATATACAAGA CTGACAATCA CCTGAAACAT TATTTACATA TCATTGAAAA CAAACCCCTG 
TATCCAGTTA TCTATGATAG CAATGGTGTC GTCCTTTCAA TGCCTCCCAT CATCAATGGG 
GATCATTCCA GAATAACAGT AAATACTAGA AATATTTTTA TTGAATGCAC GGGAACTGAC 
TTTACTAAGG CAAAAATAGT TCTTGATATT ATTGTCACCA TGTTCAGTGA ATATTGTGAG 
AATCAATTTA CGGTCGAAGC TGCTGAAGTG GTTTTTCCTA ATGGAAAATC ACATACCTTT 
CCAGAATTAG CTTACCGAAA GGAGATGGTG AGAGCTGACC TAATTAACAA AAAAGTTGGA 
ATCAGAGAAA CTCCAGAAAA TCTTGCCAAA CTTCTGACCA GGATGTATTT AAAATCAGAA 
GTCATAGGTG ATGGGAATCA GATTGAGATT GAAATCCCTC CAACCAGAGC TGACATTATC 
CATGCATGTG ATATTGTAGA AGATGCAGCT ATTGCTTATG GATATAACAA CATTCAGATG 
ACTCTCCCGA AAACTTACAC CATAGCTAAT CAATTTCCTC TTAATAAGCT CACTGAACTT 
CTCCGACATG ACATGGCAGC CGCTGGCTTC ACTGAAGCAC TTACCTTTGC CCTGTGCTCC 
CAAGAAGATA TTGCTGATAA ACTAGGTGTG GATATCTCTG CAACAAAGGC AGTCCACATA 
AGTAATCCTA AAACAGCTGA ATTTCAGGTG GCACGCACTA CCCTTCTTCC TGGCCTCCTG 
AAGACCATAG CAGCAAATCG TAAGATGCCC CTTCCACTGA AACTGTTTGA AATCTCTGAC 
ATTGTAATAA AAGATTCTAA TACAGATGTA GGTGCAAAAA ACTACAGACA TCTCTGTGCT 
GTTTATTACA ACAAGAATCC TGGGTTTGAG ATCATTCATG GGCTGCTGGA CAGAATTATG 
CAGTTGCTCG ATGTGCCTCC TGGTGAAGAC AAGGGGGGAT ATGTGATCAA AGCATCAGAA 
GGGCCTGCTT TCTTCCCCGG GCGATGTGCA GAGATCTTTG CCAGGGGTCA AAGCGTCGGG 
AAGCTTGGGG TCCTTCATCC TGACGTTATC ACCAAATTTG AGCTGACCAT GCCCTGCTCC 
TCCCTAGAAA TCAATGTTGG ACCCTTTTTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FARSB ( XM_024452492.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024308260.1 Homo sapiens phenylalanyl-tRNA synthetase subunit beta (FARSB), transcript variant X2, mRNA.

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CloneID	OHu35721
Clone ID Related Accession (Same CDS sequence)	XM_017003110.2 , XM_006712169.2 , XM_011510466.2 , XM_017003110.1 , XM_024452492.1
Accession Version	XM_024452492.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1473bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	phenylalanine--tRNA ligase beta subunit isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGATG GAAAAATCCA GAAATTGATT ATCACAGAAG AGACAGCTAA GATACGTCCT 
TTTGCGGTAG CAGCAGTTCT CCGTAATATA AAGTTTACTA AAGATCGATA TGACAGCTTC 
ATTGAACTTC AGGAGAAATT ACATCAGAAT ATTTGCAGGA AAAGAGCACT GGTTGCCATT 
GGTACCCATG ATTTGGACAC TTTGTCGGGC CCATTTACTT ATACTGCAAA GCGTCCTTCA 
GATATCAAAT TCAAGCCTCT AAATAAGACC AAGGAGTATA CAGCCTGTGA ACTGATGAAC 
ATATACAAGA CTGACAATCA CCTGAAACAT TATTTACATA TCATTGAAAA CAAACCCCTG 
TATCCAGTTA TCTATGATAG CAATGGTGTC GTCCTTTCAA TGCCTCCCAT CATCAATGGG 
GATCATTCCA GAATAACAGT AAATACTAGA AATATTTTTA TTGAATGCAC GGGAACTGAC 
TTTACTAAGG CAAAAATAGT TCTTGATATT ATTGTCACCA TGTTCAGTGA ATATTGTGAG 
AATCAATTTA CGGTCGAAGC TGCTGAAGTG GTTTTTCCTA ATGGAAAATC ACATACCTTT 
CCAGAATTAG CTTACCGAAA GGAGATGGTG AGAGCTGACC TAATTAACAA AAAAGTTGGA 
ATCAGAGAAA CTCCAGAAAA TCTTGCCAAA CTTCTGACCA GGATGTATTT AAAATCAGAA 
GTCATAGGTG ATGGGAATCA GATTGAGATT GAAATCCCTC CAACCAGAGC TGACATTATC 
CATGCATGTG ATATTGTAGA AGATGCAGCT ATTGCTTATG GATATAACAA CATTCAGATG 
ACTCTCCCGA AAACTTACAC CATAGCTAAT CAATTTCCTC TTAATAAGCT CACTGAACTT 
CTCCGACATG ACATGGCAGC CGCTGGCTTC ACTGAAGCAC TTACCTTTGC CCTGTGCTCC 
CAAGAAGATA TTGCTGATAA ACTAGGTGTG GATATCTCTG CAACAAAGGC AGTCCACATA 
AGTAATCCTA AAACAGCTGA ATTTCAGGTG GCACGCACTA CCCTTCTTCC TGGCCTCCTG 
AAGACCATAG CAGCAAATCG TAAGATGCCC CTTCCACTGA AACTGTTTGA AATCTCTGAC 
ATTGTAATAA AAGATTCTAA TACAGATGTA GGTGCAAAAA ACTACAGACA TCTCTGTGCT 
GTTTATTACA ACAAGAATCC TGGGTTTGAG ATCATTCATG GGCTGCTGGA CAGAATTATG 
CAGTTGCTCG ATGTGCCTCC TGGTGAAGAC AAGGGGGGAT ATGTGATCAA AGCATCAGAA 
GGGCCTGCTT TCTTCCCCGG GCGATGTGCA GAGATCTTTG CCAGGGGTCA AAGCGTCGGG 
AAGCTTGGGG TCCTTCATCC TGACGTTATC ACCAAATTTG AGCTGACCAT GCCCTGCTCC 
TCCCTAGAAA TCAATGTTGG ACCCTTTTTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024308260.1
CDS	831..2303
Translation	MPDGKIQKLIITEETAKIRPFAVAAVLRNIKFTKDRYDSFIELQEKLHQNICRKRALVAIGTHDLDTLSGPFTYTAKRPSDIKFKPLNKTKEYTACELMNIYKTDNHLKHYLHIIENKPLYPVIYDSNGVVLSMPPIINGDHSRITVNTRNIFIECTGTDFTKAKIVLDIIVTMFSEYCENQFTVEAAEVVFPNGKSHTFPELAYRKEMVRADLINKKVGIRETPENLAKLLTRMYLKSEVIGDGNQIEIEIPPTRADIIHACDIVEDAAIAYGYNNIQMTLPKTYTIANQFPLNKLTELLRHDMAAAGFTEALTFALCSQEDIADKLGVDISATKAVHISNPKTAEFQVARTTLLPGLLKTIAANRKMPLPLKLFEISDIVIKDSNTDVGAKNYRHLCAVYYNKNPGFEIIHGLLDRIMQLLDVPPGEDKGGYVIKASEGPAFFPGRCAEIFARGQSVGKLGVLHPDVITKFELTMPCSSLEINVGPFL

Target ORF information:

RefSeq Version	XM_024452492.1
Organism	Homo sapiens(human)
Definition	Homo sapiens phenylalanyl-tRNA synthetase subunit beta (FARSB), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024452492.1

ORF Insert Sequence:

ATGCCTGATG GAAAAATCCA GAAATTGATT ATCACAGAAG AGACAGCTAA GATACGTCCT 
TTTGCGGTAG CAGCAGTTCT CCGTAATATA AAGTTTACTA AAGATCGATA TGACAGCTTC 
ATTGAACTTC AGGAGAAATT ACATCAGAAT ATTTGCAGGA AAAGAGCACT GGTTGCCATT 
GGTACCCATG ATTTGGACAC TTTGTCGGGC CCATTTACTT ATACTGCAAA GCGTCCTTCA 
GATATCAAAT TCAAGCCTCT AAATAAGACC AAGGAGTATA CAGCCTGTGA ACTGATGAAC 
ATATACAAGA CTGACAATCA CCTGAAACAT TATTTACATA TCATTGAAAA CAAACCCCTG 
TATCCAGTTA TCTATGATAG CAATGGTGTC GTCCTTTCAA TGCCTCCCAT CATCAATGGG 
GATCATTCCA GAATAACAGT AAATACTAGA AATATTTTTA TTGAATGCAC GGGAACTGAC 
TTTACTAAGG CAAAAATAGT TCTTGATATT ATTGTCACCA TGTTCAGTGA ATATTGTGAG 
AATCAATTTA CGGTCGAAGC TGCTGAAGTG GTTTTTCCTA ATGGAAAATC ACATACCTTT 
CCAGAATTAG CTTACCGAAA GGAGATGGTG AGAGCTGACC TAATTAACAA AAAAGTTGGA 
ATCAGAGAAA CTCCAGAAAA TCTTGCCAAA CTTCTGACCA GGATGTATTT AAAATCAGAA 
GTCATAGGTG ATGGGAATCA GATTGAGATT GAAATCCCTC CAACCAGAGC TGACATTATC 
CATGCATGTG ATATTGTAGA AGATGCAGCT ATTGCTTATG GATATAACAA CATTCAGATG 
ACTCTCCCGA AAACTTACAC CATAGCTAAT CAATTTCCTC TTAATAAGCT CACTGAACTT 
CTCCGACATG ACATGGCAGC CGCTGGCTTC ACTGAAGCAC TTACCTTTGC CCTGTGCTCC 
CAAGAAGATA TTGCTGATAA ACTAGGTGTG GATATCTCTG CAACAAAGGC AGTCCACATA 
AGTAATCCTA AAACAGCTGA ATTTCAGGTG GCACGCACTA CCCTTCTTCC TGGCCTCCTG 
AAGACCATAG CAGCAAATCG TAAGATGCCC CTTCCACTGA AACTGTTTGA AATCTCTGAC 
ATTGTAATAA AAGATTCTAA TACAGATGTA GGTGCAAAAA ACTACAGACA TCTCTGTGCT 
GTTTATTACA ACAAGAATCC TGGGTTTGAG ATCATTCATG GGCTGCTGGA CAGAATTATG 
CAGTTGCTCG ATGTGCCTCC TGGTGAAGAC AAGGGGGGAT ATGTGATCAA AGCATCAGAA 
GGGCCTGCTT TCTTCCCCGG GCGATGTGCA GAGATCTTTG CCAGGGGTCA AAGCGTCGGG 
AAGCTTGGGG TCCTTCATCC TGACGTTATC ACCAAATTTG AGCTGACCAT GCCCTGCTCC 
TCCCTAGAAA TCAATGTTGG ACCCTTTTTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

Use of germline polymorphisms in predicting concurrent chemoradiotherapy response in esophageal cancer.
International journal of radiation oncology, biology, physics82(5)1996-2003(2012 Apr)
Chen PC,Chen YC,Lai LC,Tsai MH,Chen SK,Yang PW,Lee YC,Hsiao CK,Lee JM,Chuang EY

Crystal structure of human mitochondrial PheRS complexed with tRNA(Phe) in the active "open" state.
Journal of molecular biology415(3)527-37(2012 Jan)
Klipcan L,Moor N,Finarov I,Kessler N,Sukhanova M,Safro MG

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FARSB cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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