wdr77 cDNA ORF clone, Haplochromis burtoni(Burton's mouthbrooder)

Gene
Clones
gRNAs

Summary

Gene Symbol	wdr77
Entrez Gene ID	102305741
Full Name	WD repeat domain 77
Gene Type	protein-coding
Organism	Haplochromis burtoni(Burton's mouthbrooder)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005941082.1	XP_005941144.1	methylosome protein 50

Pathways from BioSystems
Gene Ontology

The following wdr77 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the wdr77 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHa26187	XM_005941082.1 Latest version!	Haplochromis burtoni WD repeat domain 77 (wdr77), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

wdr77 ( XM_005941082.1 ) cDNA ORF clone, Haplochromis burtoni(Burton's mouthbrooder) -> XP_005941144.1 Haplochromis burtoni WD repeat domain 77 (wdr77), mRNA.

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CloneID	OHa26187
Clone ID Related Accession (Same CDS sequence)	XM_005941082.1
Accession Version	XM_005941082.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1014bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-28
Organism	Haplochromis burtoni(Burton's mouthbrooder)
Product	methylosome protein 50
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005179704.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Haplochromis burtoni Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATAGTT CCGTGGATAC TAGCATGACC AAGGAAAACC GGTGGGACAT ACCCCCCAAT 
GCTCCAGCGT GCATGGAGAG GCACCTGTGC GCGGCACAAT ACAGAGCAGA TGGCACCCTG 
CTACTTGGTG CCTCCAGCCT CTCTGGCAGA AGCTGGCAGG GATCTGTTTG GATCTACAGC 
GACCCTGACC AGGCCCCCAG TGAGGGATTC TGCAAAGCTG GTGTGCAGAC TGAGGCTGGT 
GTCACAGATG TCAAATGGGT GTCAGAGAAG GGAATTATTG TTGCGTCAGA TTCAGGTGCC 
TTGGAGCTCT GGGAACTAGC AGAGGATGAA TGTCTACTGG TTAATCGCTT TACCAAGCAT 
GATCATGACC ATATTGTCAC CACAGTAAGC CCCGTGGTTG GAGCAACCGC TGCAGTCACT 
GGCAGCATGG ACTGCAGAAT TAAAGTCTGG GATCTAAGTC AGGAAACGGT TGTCACTACC 
TACAATGTGC ACACACAGCC AGTCACTTGT GTTGCCTGCA GTCCTACAGA TGAGTCTCTG 
TTTCTCTCCT GTAGCCAAGA CAGCCGTGTG TTGTTGTGGG ACAGGAGGAA GCCCAACAAA 
CCTGCATCAA GAATAGATGT AGAGTCACCT AGCTGCTCCC CTACTTCTCT AGCTTGGCAC 
CCTCACCACA GAAGCACCAT TGCTTTTGGC GATGAGCTCG GCAGGGTGAC TGTGAAAGAT 
TTCCTGGGAT CGGAGCCGTC TCAGGTGGAG AACGTTCACA GCCGTAGAGT TAATGGGCTG 
GCCTTCTCCA CACACAGCAC ACCCTTGCTA GCCTCTGTTA GTGATGACTG TAGCGTTGCT 
GTTATGGACT CTGAACTGCG AGAAATACTC AGAGACCGGC GGCACCAGGA CTATGTCAAA 
GGTGTGAGCT GGCTTCACAA TGGCTCCTCC ACCCTCACAA CAGTAGGCTG GGATCATCTT 
GTGCTTCACC ATAAAGTGGG TCCAGCTGAT GGCATTCCCA GTTCCTCTTC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005941144.1
CDS	308..1321
Translation	MNSSVDTSMTKENRWDIPPNAPACMERHLCAAQYRADGTLLLGASSLSGRSWQGSVWIYSDPDQAPSEGFCKAGVQTEAGVTDVKWVSEKGIIVASDSGALELWELAEDECLLVNRFTKHDHDHIVTTVSPVVGATAAVTGSMDCRIKVWDLSQETVVTTYNVHTQPVTCVACSPTDESLFLSCSQDSRVLLWDRRKPNKPASRIDVESPSCSPTSLAWHPHHRSTIAFGDELGRVTVKDFLGSEPSQVENVHSRRVNGLAFSTHSTPLLASVSDDCSVAVMDSELREILRDRRHQDYVKGVSWLHNGSSTLTTVGWDHLVLHHKVGPADGIPSSSS

Target ORF information:

RefSeq Version	XM_005941082.1
Organism	Haplochromis burtoni(Burton's mouthbrooder)
Definition	Haplochromis burtoni WD repeat domain 77 (wdr77), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005941082.1

ORF Insert Sequence:

ATGAATAGTT CCGTGGATAC TAGCATGACC AAGGAAAACC GGTGGGACAT ACCCCCCAAT 
GCTCCAGCGT GCATGGAGAG GCACCTGTGC GCGGCACAAT ACAGAGCAGA TGGCACCCTG 
CTACTTGGTG CCTCCAGCCT CTCTGGCAGA AGCTGGCAGG GATCTGTTTG GATCTACAGC 
GACCCTGACC AGGCCCCCAG TGAGGGATTC TGCAAAGCTG GTGTGCAGAC TGAGGCTGGT 
GTCACAGATG TCAAATGGGT GTCAGAGAAG GGAATTATTG TTGCGTCAGA TTCAGGTGCC 
TTGGAGCTCT GGGAACTAGC AGAGGATGAA TGTCTACTGG TTAATCGCTT TACCAAGCAT 
GATCATGACC ATATTGTCAC CACAGTAAGC CCCGTGGTTG GAGCAACCGC TGCAGTCACT 
GGCAGCATGG ACTGCAGAAT TAAAGTCTGG GATCTAAGTC AGGAAACGGT TGTCACTACC 
TACAATGTGC ACACACAGCC AGTCACTTGT GTTGCCTGCA GTCCTACAGA TGAGTCTCTG 
TTTCTCTCCT GTAGCCAAGA CAGCCGTGTG TTGTTGTGGG ACAGGAGGAA GCCCAACAAA 
CCTGCATCAA GAATAGATGT AGAGTCACCT AGCTGCTCCC CTACTTCTCT AGCTTGGCAC 
CCTCACCACA GAAGCACCAT TGCTTTTGGC GATGAGCTCG GCAGGGTGAC TGTGAAAGAT 
TTCCTGGGAT CGGAGCCGTC TCAGGTGGAG AACGTTCACA GCCGTAGAGT TAATGGGCTG 
GCCTTCTCCA CACACAGCAC ACCCTTGCTA GCCTCTGTTA GTGATGACTG TAGCGTTGCT 
GTTATGGACT CTGAACTGCG AGAAATACTC AGAGACCGGC GGCACCAGGA CTATGTCAAA 
GGTGTGAGCT GGCTTCACAA TGGCTCCTCC ACCCTCACAA CAGTAGGCTG GGATCATCTT 
GTGCTTCACC ATAAAGTGGG TCCAGCTGAT GGCATTCCCA GTTCCTCTTC TTAG