mief1 cDNA ORF clone, Haplochromis burtoni(Burton's mouthbrooder)

Gene
Clones
gRNAs

Summary

Gene Symbol	mief1
Entrez Gene ID	102297084
Full Name	mitochondrial elongation factor 1
Gene Type	protein-coding
Organism	Haplochromis burtoni(Burton's mouthbrooder)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005946183.2	XP_005946245.1	mitochondrial dynamics protein MID51

Pathways from BioSystems
Gene Ontology

The following mief1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mief1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHa30700	XM_005946183.2 Latest version!	Haplochromis burtoni mitochondrial elongation factor 1 (mief1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

mief1 ( XM_005946183.2 ) cDNA ORF clone, Haplochromis burtoni(Burton's mouthbrooder) -> XP_005946245.1 Haplochromis burtoni mitochondrial elongation factor 1 (mief1), mRNA.

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CloneID	OHa30700
Clone ID Related Accession (Same CDS sequence)	XM_005946183.2
Accession Version	XM_005946183.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1437bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-28
Organism	Haplochromis burtoni(Burton's mouthbrooder)
Product	mitochondrial dynamics protein MID51
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005179884.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 29, 2015 this sequence version replaced XM_005946183.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Haplochromis burtoni Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGGGG TGAACGGAGA CCGAAAAGGA AAGAAGGATG ATAATGGGAT TGGAACAGCT 
ATTGACTTCA TGCTTTCAAA TGCCAAACTT GTGCTGGGGG TGGGAGGGGC AGCCATGCTT 
GGCATTGCAA CACTAGCCGT CAAAAGAATG TATGATCGTG CCATAAGTGC TCCCACCAGC 
CCCACAAAGA TGGAGCAGAC AGGAAAGAGA AGCTGGGAGG AGCCTGCCTG GATGGGGTCG 
TCCCCGAGGG TTTTAAACCA TGACATGAAG TCCACAGTTA GCAGGTCGCT GCAGTCTCTG 
CCCACTTCTT CACATGCTTT TGAACCAGAC TGTCTGCGAA GGGCTGTGGG TCGAGCTGCT 
GTGGGAAGAA GCGATGCCAC CCGGTCGGAT ATGATGCGGG CCCGGATGCG TCTGTCTTTG 
CAAGAACACC TGTGGGAATT CTACCAAAAT AATGTCAACA TCCCTGCTGA GGAGCAGGCC 
ATGGCCAGGA GGGCGGCGCT GGACATCTGC GCTGAGCTCA GAGTGTTTCT TCATGCCAAA 
CTGCCTGACA TGCCGCTCAG AGAGATGTAC CTCAGTGGCA GTCTGTACGA TGACCTTCAG 
GTTGTAACAG CCGACCACGC CCAGCTCATG GTGCCTCTTG TCTTGGAGAA GAATCTGTGG 
TCGTCCATCC CCGGAGAGGA CACGATCATG AATGTCCCAG GTTTCTGGCT TGTCCGTAGG 
GAGAATTTGG AATATTTCCC TCGGAGCAGC AGCTATTGGG ACCGATGCAT GGTCGGAGGT 
TATCTCTCCC CGAAATCAGT GCTTGAAGTC TTTGAGAAGC TTGTTGCAGG TTCTATTAAC 
TGGCCAGCTA TAGGGAGTGT CCTCGACTAT ATCATCCGTC CCGTGGTTCC CGCCGAAACG 
CTGACTCTGG AGGTACAGTA CGAGACGGAC AGGAAGCTGT ATGTGGACTT CTTGCCTTTG 
CTCGTGATGG AGGATGGAAC CTCACTGATT GCCAAACCGC ACCGGTTTGC TGCAGAGCGC 
CACGAAAACC TGTGGCGGCA GAGCTTCCGC GTGGCCGAGA CCGCGCGGCT CAGGGCACTA 
GATCAGGAGG ATGGCGGCTG CCGCTGCACC TGCCTCAAAG TGGCGAAGGC TGTGTGCAAG 
CTCAACCCTG CCCTTCACAA GCTCAACTCC AGCCAGCTCA CTAATACCAT CCTGTTGCTC 
AGTGAAAAAG AAGGTGACTG GACTCAGGAA GCGCTGGCCG ACCGCTTCCT TCAGCTGCTA 
CGAGCGCTAG TGGGGCACTT GGAGGCTGGG AAGCTGCCGT GTGCCCTTAG CCCTAAAGTG 
AACTTGTTTT GTGAGCTGAC TGATCAAGAA GTGGACGAGC TAGGGTATAC CCTCTACTGT 
GCGCTGTCTG ATCCCGGGGG ACTGCTGAGA ACTGCAGCAC AGCCACCACA CCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005946245.1
CDS	412..1848
Translation	MAGVNGDRKGKKDDNGIGTAIDFMLSNAKLVLGVGGAAMLGIATLAVKRMYDRAISAPTSPTKMEQTGKRSWEEPAWMGSSPRVLNHDMKSTVSRSLQSLPTSSHAFEPDCLRRAVGRAAVGRSDATRSDMMRARMRLSLQEHLWEFYQNNVNIPAEEQAMARRAALDICAELRVFLHAKLPDMPLREMYLSGSLYDDLQVVTADHAQLMVPLVLEKNLWSSIPGEDTIMNVPGFWLVRRENLEYFPRSSSYWDRCMVGGYLSPKSVLEVFEKLVAGSINWPAIGSVLDYIIRPVVPAETLTLEVQYETDRKLYVDFLPLLVMEDGTSLIAKPHRFAAERHENLWRQSFRVAETARLRALDQEDGGCRCTCLKVAKAVCKLNPALHKLNSSQLTNTILLLSEKEGDWTQEALADRFLQLLRALVGHLEAGKLPCALSPKVNLFCELTDQEVDELGYTLYCALSDPGGLLRTAAQPPHL

Target ORF information:

RefSeq Version	XM_005946183.2
Organism	Haplochromis burtoni(Burton's mouthbrooder)
Definition	Haplochromis burtoni mitochondrial elongation factor 1 (mief1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005946183.2

ORF Insert Sequence:

ATGGCGGGGG TGAACGGAGA CCGAAAAGGA AAGAAGGATG ATAATGGGAT TGGAACAGCT 
ATTGACTTCA TGCTTTCAAA TGCCAAACTT GTGCTGGGGG TGGGAGGGGC AGCCATGCTT 
GGCATTGCAA CACTAGCCGT CAAAAGAATG TATGATCGTG CCATAAGTGC TCCCACCAGC 
CCCACAAAGA TGGAGCAGAC AGGAAAGAGA AGCTGGGAGG AGCCTGCCTG GATGGGGTCG 
TCCCCGAGGG TTTTAAACCA TGACATGAAG TCCACAGTTA GCAGGTCGCT GCAGTCTCTG 
CCCACTTCTT CACATGCTTT TGAACCAGAC TGTCTGCGAA GGGCTGTGGG TCGAGCTGCT 
GTGGGAAGAA GCGATGCCAC CCGGTCGGAT ATGATGCGGG CCCGGATGCG TCTGTCTTTG 
CAAGAACACC TGTGGGAATT CTACCAAAAT AATGTCAACA TCCCTGCTGA GGAGCAGGCC 
ATGGCCAGGA GGGCGGCGCT GGACATCTGC GCTGAGCTCA GAGTGTTTCT TCATGCCAAA 
CTGCCTGACA TGCCGCTCAG AGAGATGTAC CTCAGTGGCA GTCTGTACGA TGACCTTCAG 
GTTGTAACAG CCGACCACGC CCAGCTCATG GTGCCTCTTG TCTTGGAGAA GAATCTGTGG 
TCGTCCATCC CCGGAGAGGA CACGATCATG AATGTCCCAG GTTTCTGGCT TGTCCGTAGG 
GAGAATTTGG AATATTTCCC TCGGAGCAGC AGCTATTGGG ACCGATGCAT GGTCGGAGGT 
TATCTCTCCC CGAAATCAGT GCTTGAAGTC TTTGAGAAGC TTGTTGCAGG TTCTATTAAC 
TGGCCAGCTA TAGGGAGTGT CCTCGACTAT ATCATCCGTC CCGTGGTTCC CGCCGAAACG 
CTGACTCTGG AGGTACAGTA CGAGACGGAC AGGAAGCTGT ATGTGGACTT CTTGCCTTTG 
CTCGTGATGG AGGATGGAAC CTCACTGATT GCCAAACCGC ACCGGTTTGC TGCAGAGCGC 
CACGAAAACC TGTGGCGGCA GAGCTTCCGC GTGGCCGAGA CCGCGCGGCT CAGGGCACTA 
GATCAGGAGG ATGGCGGCTG CCGCTGCACC TGCCTCAAAG TGGCGAAGGC TGTGTGCAAG 
CTCAACCCTG CCCTTCACAA GCTCAACTCC AGCCAGCTCA CTAATACCAT CCTGTTGCTC 
AGTGAAAAAG AAGGTGACTG GACTCAGGAA GCGCTGGCCG ACCGCTTCCT TCAGCTGCTA 
CGAGCGCTAG TGGGGCACTT GGAGGCTGGG AAGCTGCCGT GTGCCCTTAG CCCTAAAGTG 
AACTTGTTTT GTGAGCTGAC TGATCAAGAA GTGGACGAGC TAGGGTATAC CCTCTACTGT 
GCGCTGTCTG ATCCCGGGGG ACTGCTGAGA ACTGCAGCAC AGCCACCACA CCTCTGA