tkfc cDNA ORF clone, Haplochromis burtoni(Burton's mouthbrooder)

Gene
Clones
gRNAs

Summary

Gene Symbol	tkfc
Entrez Gene ID	102292656
Full Name	triokinase and FMN cyclase
Gene Type	protein-coding
Organism	Haplochromis burtoni(Burton's mouthbrooder)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005946346.2	XP_005946408.2	triokinase/FMN cyclase

Pathways from BioSystems
Gene Ontology

The following tkfc gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tkfc cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHa30834	XM_005946346.2 Latest version!	Haplochromis burtoni triokinase/FMN cyclase (tkfc), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

tkfc ( XM_005946346.2 ) cDNA ORF clone, Haplochromis burtoni(Burton's mouthbrooder) -> XP_005946408.2 Haplochromis burtoni triokinase/FMN cyclase (tkfc), mRNA.

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CloneID	OHa30834
Clone ID Related Accession (Same CDS sequence)	XM_005946346.2
Accession Version	XM_005946346.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1743bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-28
Organism	Haplochromis burtoni(Burton's mouthbrooder)
Product	triokinase/FMN cyclase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005179891.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 29, 2015 this sequence version replaced XM_005946346.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Haplochromis burtoni Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCCC AGAAGAAGTT GATCAACTCA GCAGAGAGTT GCGTGGATGA CGCCCTCCTC 
GGCCTCGTCA GGGCCTCTGG GGGCCTCTCT CTGCTAAAGG GCCACAGGGT TGTGCTTCGG 
TCTGACCTTG AAAACCTCAA GGGAAAAGTG GCCCTCCTCT CTGGAGGGGG GTCGGGCCAC 
GAGCCTGCAC ACGCAGGTTA TATCGGAGCA GGGATGCTGT CGGCGGCTGT TGCAGGAGGA 
GTGTTTGCAT CGCCGCCTCC TGGCAGTATC CTTGCTGCCA TTCTCTCCTT GCACAATGCA 
GGAGCCTCTG GGGTCCTTCT CATTGTGAAG AACTACACAG GTGACCGGCT TAACTTTGGA 
TTGGCTGCAG AGCAAGCTCG TAACCAGGGC GTTGCTGTTG ACATGGTGAT AGTTGCAGAA 
GACTGCGCCT TTGACCAACC AAGTAAGGCT GGAAGAAGAG GCTTGTGTGG CACTGTCTTC 
ATACATAAGC TGGCTGGTGC TTTAGCAGAA GAGGGCTGCT CATTGGATCA GATTGTTTCC 
AAGGTGACGG AGATTTTAAA GAGGATCGGC ACTCTGGGGG TGAGTCTGTC TCCATGCAGC 
GTTCCCGGCT GCCTGCCTTC CTTCGATCTA CCGCCAGGAG ACATGGAGGT CGGGCTGGGT 
ATCCACGGGG AACCTGGAAT AAAGAGGTCA AAGGTGGCCT CTGCTGATGA GGTGGTCAAG 
ACCATGATAG ACCACATGAC CAACCCAAGC AGCCAATTGC ATCTGCCGCT GAAATCAGGA 
GACAGTGTGG TTGTGTGCGT GAACAACCTT GGAGCTCTGT CTTGCCTAGA GATGGCTGTG 
GTGACTAGAT CGGCCATCAT CTGCCTGGAA AGCCGTGGAG TGGTGGTTGC CAGGGTGATG 
TCTGGGTTGT TCATGACCTC TCTGGAGATG GCAGGAGTGT CGCTGACCCT GATGAGGGCT 
GACAAGGAAA CACTGAGGCT GATAGATGCT AAGACTACTG CCCCTGCCTG GCCAAACCTC 
AGCAATGTGT GTTTGAGTGG ACGCAGCTAC ATCACAGAAC CACAAACAAC CATCAAACGG 
CCACAAGATG AGAAGCACTC TGAAGGACCA CTTAGTCCTG TGATGCATAA AGCCTTGGAG 
AAGATTTGCT CCACTCTGTT GGAAAAGCAG GAGGAACTCA ATTCCCTTGA TCGTGCGTCC 
GGGGATGGAG ACTGTGGTAA TACTCATGCT CAGGCTGCTA GAGCCATTCA GGAGTGGCTT 
CAAGGACATG TGGTCCCCGG TTGCCCCGGG AAGCTGTTAT CAGTCGTTGC TGGATTGGTG 
CAGGAGAAAA TGGGCGGGTC TTCAGGAGCG TTGTACAGTC TGTTCCTCAC TGCGGCAGCC 
GGTCATGTGA CCGAGGGGCG GAGCAATGCT GCAGCCTGGG CCACTGCTAT GAATGCTGGG 
ACAGAAGCAA TGAAAAAATA TGGTGGTGCC GCCCCTGGAG ACAGAACAAT GCTGGATGCT 
TTGTGCCCTG CAGTTGATGA ACTGATTAAG CTGGCCACAG CTCCATCTGG TGGACACATG 
GCTGTACTGC AAAGTGCTGT GAAGAAAGCT GCTTCAGGAG CGGAGGCAAC TCGTGACCTT 
AAAGCAAGGG CTGGGAGAGC CAGCTACATC TCACCCAACC TGGTCAGCCT GCCAGACCCT 
GGTGCTGTAG CTGTGGCAAC CATCTTGAAA GCTATTCTGG AGGTGCTAGA GGGGCGAAAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005946408.2
CDS	102..1844
Translation	MEPQKKLINSAESCVDDALLGLVRASGGLSLLKGHRVVLRSDLENLKGKVALLSGGGSGHEPAHAGYIGAGMLSAAVAGGVFASPPPGSILAAILSLHNAGASGVLLIVKNYTGDRLNFGLAAEQARNQGVAVDMVIVAEDCAFDQPSKAGRRGLCGTVFIHKLAGALAEEGCSLDQIVSKVTEILKRIGTLGVSLSPCSVPGCLPSFDLPPGDMEVGLGIHGEPGIKRSKVASADEVVKTMIDHMTNPSSQLHLPLKSGDSVVVCVNNLGALSCLEMAVVTRSAIICLESRGVVVARVMSGLFMTSLEMAGVSLTLMRADKETLRLIDAKTTAPAWPNLSNVCLSGRSYITEPQTTIKRPQDEKHSEGPLSPVMHKALEKICSTLLEKQEELNSLDRASGDGDCGNTHAQAARAIQEWLQGHVVPGCPGKLLSVVAGLVQEKMGGSSGALYSLFLTAAAGHVTEGRSNAAAWATAMNAGTEAMKKYGGAAPGDRTMLDALCPAVDELIKLATAPSGGHMAVLQSAVKKAASGAEATRDLKARAGRASYISPNLVSLPDPGAVAVATILKAILEVLEGRK

Target ORF information:

RefSeq Version	XM_005946346.2
Organism	Haplochromis burtoni(Burton's mouthbrooder)
Definition	Haplochromis burtoni triokinase/FMN cyclase (tkfc), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005946346.2

ORF Insert Sequence:

ATGGAGCCCC AGAAGAAGTT GATCAACTCA GCAGAGAGTT GCGTGGATGA CGCCCTCCTC 
GGCCTCGTCA GGGCCTCTGG GGGCCTCTCT CTGCTAAAGG GCCACAGGGT TGTGCTTCGG 
TCTGACCTTG AAAACCTCAA GGGAAAAGTG GCCCTCCTCT CTGGAGGGGG GTCGGGCCAC 
GAGCCTGCAC ACGCAGGTTA TATCGGAGCA GGGATGCTGT CGGCGGCTGT TGCAGGAGGA 
GTGTTTGCAT CGCCGCCTCC TGGCAGTATC CTTGCTGCCA TTCTCTCCTT GCACAATGCA 
GGAGCCTCTG GGGTCCTTCT CATTGTGAAG AACTACACAG GTGACCGGCT TAACTTTGGA 
TTGGCTGCAG AGCAAGCTCG TAACCAGGGC GTTGCTGTTG ACATGGTGAT AGTTGCAGAA 
GACTGCGCCT TTGACCAACC AAGTAAGGCT GGAAGAAGAG GCTTGTGTGG CACTGTCTTC 
ATACATAAGC TGGCTGGTGC TTTAGCAGAA GAGGGCTGCT CATTGGATCA GATTGTTTCC 
AAGGTGACGG AGATTTTAAA GAGGATCGGC ACTCTGGGGG TGAGTCTGTC TCCATGCAGC 
GTTCCCGGCT GCCTGCCTTC CTTCGATCTA CCGCCAGGAG ACATGGAGGT CGGGCTGGGT 
ATCCACGGGG AACCTGGAAT AAAGAGGTCA AAGGTGGCCT CTGCTGATGA GGTGGTCAAG 
ACCATGATAG ACCACATGAC CAACCCAAGC AGCCAATTGC ATCTGCCGCT GAAATCAGGA 
GACAGTGTGG TTGTGTGCGT GAACAACCTT GGAGCTCTGT CTTGCCTAGA GATGGCTGTG 
GTGACTAGAT CGGCCATCAT CTGCCTGGAA AGCCGTGGAG TGGTGGTTGC CAGGGTGATG 
TCTGGGTTGT TCATGACCTC TCTGGAGATG GCAGGAGTGT CGCTGACCCT GATGAGGGCT 
GACAAGGAAA CACTGAGGCT GATAGATGCT AAGACTACTG CCCCTGCCTG GCCAAACCTC 
AGCAATGTGT GTTTGAGTGG ACGCAGCTAC ATCACAGAAC CACAAACAAC CATCAAACGG 
CCACAAGATG AGAAGCACTC TGAAGGACCA CTTAGTCCTG TGATGCATAA AGCCTTGGAG 
AAGATTTGCT CCACTCTGTT GGAAAAGCAG GAGGAACTCA ATTCCCTTGA TCGTGCGTCC 
GGGGATGGAG ACTGTGGTAA TACTCATGCT CAGGCTGCTA GAGCCATTCA GGAGTGGCTT 
CAAGGACATG TGGTCCCCGG TTGCCCCGGG AAGCTGTTAT CAGTCGTTGC TGGATTGGTG 
CAGGAGAAAA TGGGCGGGTC TTCAGGAGCG TTGTACAGTC TGTTCCTCAC TGCGGCAGCC 
GGTCATGTGA CCGAGGGGCG GAGCAATGCT GCAGCCTGGG CCACTGCTAT GAATGCTGGG 
ACAGAAGCAA TGAAAAAATA TGGTGGTGCC GCCCCTGGAG ACAGAACAAT GCTGGATGCT 
TTGTGCCCTG CAGTTGATGA ACTGATTAAG CTGGCCACAG CTCCATCTGG TGGACACATG 
GCTGTACTGC AAAGTGCTGT GAAGAAAGCT GCTTCAGGAG CGGAGGCAAC TCGTGACCTT 
AAAGCAAGGG CTGGGAGAGC CAGCTACATC TCACCCAACC TGGTCAGCCT GCCAGACCCT 
GGTGCTGTAG CTGTGGCAAC CATCTTGAAA GCTATTCTGG AGGTGCTAGA GGGGCGAAAG 
TGA