EEFSEC cDNA ORF clone, Gavialis gangeticus(Gharial)

Gene
Clones
gRNAs

Summary

Gene Symbol	EEFSEC
Entrez Gene ID	109289063
Full Name	eukaryotic elongation factor, selenocysteine-tRNA specific
Gene Type	protein-coding
Organism	Gavialis gangeticus(Gharial)

mRNA and Protein(s)

mRNA	Protein	Name
XM_019504946.1	XP_019360491.1	selenocysteine-specific elongation factor isoform X1
XM_019504948.1	XP_019360493.1	selenocysteine-specific elongation factor isoform X2

Pathways from BioSystems
Gene Ontology

The following EEFSEC gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EEFSEC cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OGa68810	XM_019504948.1 Latest version!	Gavialis gangeticus eukaryotic elongation factor, selenocysteine-tRNA specific (EEFSEC), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00
OGa68809	XM_019504946.1 Latest version!	Gavialis gangeticus eukaryotic elongation factor, selenocysteine-tRNA specific (EEFSEC), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

EEFSEC ( XM_019504948.1 ) cDNA ORF clone, Gavialis gangeticus(Gharial) -> XP_019360493.1 Gavialis gangeticus eukaryotic elongation factor, selenocysteine-tRNA specific (EEFSEC), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OGa68810
Clone ID Related Accession (Same CDS sequence)	XM_019504948.1
Accession Version	XM_019504948.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1431bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-15
Organism	Gavialis gangeticus(Gharial)
Product	selenocysteine-specific elongation factor isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017728997.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gavialis gangeticus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGCTTG TAATAGATGT GACAAAAGGG ATGCAGACTC AGTCAGCAGA GTGCTTGGTG 
ATTGGACAAA TTGCTTGCCA GAAGATGGTA GTAGTTCTGA ACAAAATAGA TCTCCTTCCA 
GAGGGGAAGA GACAGGCTGC CATTGAGAAG ATGACCAAGA AAATGCAAAA GACCTTGGAA 
AATACAAAGT TCCATGGGTG TCCTATTGTT GCAGTGGCAG CTAAACCAGG TGGACCAGAA 
GCCCCAGAGA CTGTGAATCC ACTAGGTGTT TCTGAATTAA TTGAGGTCCT GAAGTCTCAG 
GCTTACCTGC CATCAAGAGA TCCCTCTGGA CCCTTCCTTA TGGCAGTTGA TCACTGTTTC 
TCTATCAAGG GTCAAGGCAC AGTGATGACA GGGACTATTC TTACTGGCTC TGTTAGCCTA 
GGTGATAATG TGGAGATTCC CGCCCTGAAG GTGTCAAAGA AAGTCAAGTC CATGCAGATG 
TTCCATACTC CTGTGACTTA CGCCATGCAG GGGGACAGGG TAGGCATCTG TGTGACCCAG 
TTTGATCCCA AGCTGCTAGA ACGGGGCCTC ATCTGTACCC CAGAATCTCT CCAGACCATA 
CATGCTGCCA TTATCTCCCT GAAGAAAATT CAGTATTTTC GAGGTGCGCT GCACACCAAA 
GCCAAGTTTC ATATCACAGT TGGTCACGAA ACAGTTATGG GACGAGTAAT GTTCTTCAGT 
CCAGCCCCTG CTGACTTCAA CAAAGAGCCC CTGGACAACG CGTTTGATTT TGAAAAAGAT 
TACCTTTATC AAGAAGAATA TTTGTCCAAG GATTCAAAGG CTTCTGATGA AAACAAGGAT 
AATGACCAGA CAGGAGAGCA GCTCCCGAAG CAACAGTGGG CCTTAGTGGA GTTTGAAAAA 
CCAGTCACTT GTCCTCGACT CTGTCTTGTA ATTGGGTCAA AACTGGATAC TGATATTCAT 
GCAAACACCT GCAGGCTGGC ATTCCATGGG GTACTGCTTC AAGGCCTAGA AGATAAAAAC 
TATACAGAGA CTTTCCTTCC AAAGCTGAAA GTGTACAAAC TGAAGCACAA AGAAGGTCAA 
GTGGAAAGGG TGATAGATGA TTACAGCGTG ATCGGTCGTT CGCTGTTTAA AAAGGAAAGC 
AACATCCAGA TTTTTGTGGG GCTGAAAGTC AAGCTATCTA CAGGAGAGGA TGGCATAATA 
GAGGGTGGCT TTGGGCAGAG TGGTAAATTT AAAATCCGAA TCCCAGATGG GCTCAAGCCA 
GACACAAAGG CACTTCTGAC TGTTGCCTCT AAGAAGAAAT CCAGAGCAGG GAAGGGGGAT 
GCAGCCAAAG AGGATGACAG CACCAAAACT GAGTCAGCCC AGCCTGTTGC CATCAGCCTT 
CTCTTTAAAA GATATGTCTT TGACACGCAG AAGAAAATGA TTCAGTCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019360493.1
CDS	95..1525
Translation	MMLVIDVTKGMQTQSAECLVIGQIACQKMVVVLNKIDLLPEGKRQAAIEKMTKKMQKTLENTKFHGCPIVAVAAKPGGPEAPETVNPLGVSELIEVLKSQAYLPSRDPSGPFLMAVDHCFSIKGQGTVMTGTILTGSVSLGDNVEIPALKVSKKVKSMQMFHTPVTYAMQGDRVGICVTQFDPKLLERGLICTPESLQTIHAAIISLKKIQYFRGALHTKAKFHITVGHETVMGRVMFFSPAPADFNKEPLDNAFDFEKDYLYQEEYLSKDSKASDENKDNDQTGEQLPKQQWALVEFEKPVTCPRLCLVIGSKLDTDIHANTCRLAFHGVLLQGLEDKNYTETFLPKLKVYKLKHKEGQVERVIDDYSVIGRSLFKKESNIQIFVGLKVKLSTGEDGIIEGGFGQSGKFKIRIPDGLKPDTKALLTVASKKKSRAGKGDAAKEDDSTKTESAQPVAISLLFKRYVFDTQKKMIQS

Target ORF information:

RefSeq Version	XM_019504948.1
Organism	Gavialis gangeticus(Gharial)
Definition	Gavialis gangeticus eukaryotic elongation factor, selenocysteine-tRNA specific (EEFSEC), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019504948.1

ORF Insert Sequence:

ATGATGCTTG TAATAGATGT GACAAAAGGG ATGCAGACTC AGTCAGCAGA GTGCTTGGTG 
ATTGGACAAA TTGCTTGCCA GAAGATGGTA GTAGTTCTGA ACAAAATAGA TCTCCTTCCA 
GAGGGGAAGA GACAGGCTGC CATTGAGAAG ATGACCAAGA AAATGCAAAA GACCTTGGAA 
AATACAAAGT TCCATGGGTG TCCTATTGTT GCAGTGGCAG CTAAACCAGG TGGACCAGAA 
GCCCCAGAGA CTGTGAATCC ACTAGGTGTT TCTGAATTAA TTGAGGTCCT GAAGTCTCAG 
GCTTACCTGC CATCAAGAGA TCCCTCTGGA CCCTTCCTTA TGGCAGTTGA TCACTGTTTC 
TCTATCAAGG GTCAAGGCAC AGTGATGACA GGGACTATTC TTACTGGCTC TGTTAGCCTA 
GGTGATAATG TGGAGATTCC CGCCCTGAAG GTGTCAAAGA AAGTCAAGTC CATGCAGATG 
TTCCATACTC CTGTGACTTA CGCCATGCAG GGGGACAGGG TAGGCATCTG TGTGACCCAG 
TTTGATCCCA AGCTGCTAGA ACGGGGCCTC ATCTGTACCC CAGAATCTCT CCAGACCATA 
CATGCTGCCA TTATCTCCCT GAAGAAAATT CAGTATTTTC GAGGTGCGCT GCACACCAAA 
GCCAAGTTTC ATATCACAGT TGGTCACGAA ACAGTTATGG GACGAGTAAT GTTCTTCAGT 
CCAGCCCCTG CTGACTTCAA CAAAGAGCCC CTGGACAACG CGTTTGATTT TGAAAAAGAT 
TACCTTTATC AAGAAGAATA TTTGTCCAAG GATTCAAAGG CTTCTGATGA AAACAAGGAT 
AATGACCAGA CAGGAGAGCA GCTCCCGAAG CAACAGTGGG CCTTAGTGGA GTTTGAAAAA 
CCAGTCACTT GTCCTCGACT CTGTCTTGTA ATTGGGTCAA AACTGGATAC TGATATTCAT 
GCAAACACCT GCAGGCTGGC ATTCCATGGG GTACTGCTTC AAGGCCTAGA AGATAAAAAC 
TATACAGAGA CTTTCCTTCC AAAGCTGAAA GTGTACAAAC TGAAGCACAA AGAAGGTCAA 
GTGGAAAGGG TGATAGATGA TTACAGCGTG ATCGGTCGTT CGCTGTTTAA AAAGGAAAGC 
AACATCCAGA TTTTTGTGGG GCTGAAAGTC AAGCTATCTA CAGGAGAGGA TGGCATAATA 
GAGGGTGGCT TTGGGCAGAG TGGTAAATTT AAAATCCGAA TCCCAGATGG GCTCAAGCCA 
GACACAAAGG CACTTCTGAC TGTTGCCTCT AAGAAGAAAT CCAGAGCAGG GAAGGGGGAT 
GCAGCCAAAG AGGATGACAG CACCAAAACT GAGTCAGCCC AGCCTGTTGC CATCAGCCTT 
CTCTTTAAAA GATATGTCTT TGACACGCAG AAGAAAATGA TTCAGTCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EEFSEC ( XM_019504946.1 ) cDNA ORF clone, Gavialis gangeticus(Gharial) -> XP_019360491.1 Gavialis gangeticus eukaryotic elongation factor, selenocysteine-tRNA specific (EEFSEC), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OGa68809
Clone ID Related Accession (Same CDS sequence)	XM_019504946.1
Accession Version	XM_019504946.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1734bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-15
Organism	Gavialis gangeticus(Gharial)
Product	selenocysteine-specific elongation factor isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017728997.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gavialis gangeticus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGTGG CTGAGGAGCC GGGGCTGGGG CGGCTGAACG TGAATGTGGG CGTCCTGGGC 
CACATCGACA GCGGCAAGAC GGCGCTGGCC CGAGCGCTCA GCACCGCCAG CTCCACCGCC 
GCCTTCGACC GCGCGCCGCA GAGCCGCGCC CGCGGCATCA CCCTGGACCT GGGCTTCTCG 
TGCCTCTGCA CCGCGCTGCC CCCGCGCCTC GGCCCCGGCC ATCTGCAGTT CACGCTGGTG 
GACTGCCCGG GCCACGCCTC CCTCATCCGG ACCATCATCG GCGGTGCCCA GATTATTGAT 
TTGATGATGC TTGTAATAGA TGTGACAAAA GGGATGCAGA CTCAGTCAGC AGAGTGCTTG 
GTGATTGGAC AAATTGCTTG CCAGAAGATG GTAGTAGTTC TGAACAAAAT AGATCTCCTT 
CCAGAGGGGA AGAGACAGGC TGCCATTGAG AAGATGACCA AGAAAATGCA AAAGACCTTG 
GAAAATACAA AGTTCCATGG GTGTCCTATT GTTGCAGTGG CAGCTAAACC AGGTGGACCA 
GAAGCCCCAG AGACTGTGAA TCCACTAGGT GTTTCTGAAT TAATTGAGGT CCTGAAGTCT 
CAGGCTTACC TGCCATCAAG AGATCCCTCT GGACCCTTCC TTATGGCAGT TGATCACTGT 
TTCTCTATCA AGGGTCAAGG CACAGTGATG ACAGGGACTA TTCTTACTGG CTCTGTTAGC 
CTAGGTGATA ATGTGGAGAT TCCCGCCCTG AAGGTGTCAA AGAAAGTCAA GTCCATGCAG 
ATGTTCCATA CTCCTGTGAC TTACGCCATG CAGGGGGACA GGGTAGGCAT CTGTGTGACC 
CAGTTTGATC CCAAGCTGCT AGAACGGGGC CTCATCTGTA CCCCAGAATC TCTCCAGACC 
ATACATGCTG CCATTATCTC CCTGAAGAAA ATTCAGTATT TTCGAGGTGC GCTGCACACC 
AAAGCCAAGT TTCATATCAC AGTTGGTCAC GAAACAGTTA TGGGACGAGT AATGTTCTTC 
AGTCCAGCCC CTGCTGACTT CAACAAAGAG CCCCTGGACA ACGCGTTTGA TTTTGAAAAA 
GATTACCTTT ATCAAGAAGA ATATTTGTCC AAGGATTCAA AGGCTTCTGA TGAAAACAAG 
GATAATGACC AGACAGGAGA GCAGCTCCCG AAGCAACAGT GGGCCTTAGT GGAGTTTGAA 
AAACCAGTCA CTTGTCCTCG ACTCTGTCTT GTAATTGGGT CAAAACTGGA TACTGATATT 
CATGCAAACA CCTGCAGGCT GGCATTCCAT GGGGTACTGC TTCAAGGCCT AGAAGATAAA 
AACTATACAG AGACTTTCCT TCCAAAGCTG AAAGTGTACA AACTGAAGCA CAAAGAAGGT 
CAAGTGGAAA GGGTGATAGA TGATTACAGC GTGATCGGTC GTTCGCTGTT TAAAAAGGAA 
AGCAACATCC AGATTTTTGT GGGGCTGAAA GTCAAGCTAT CTACAGGAGA GGATGGCATA 
ATAGAGGGTG GCTTTGGGCA GAGTGGTAAA TTTAAAATCC GAATCCCAGA TGGGCTCAAG 
CCAGACACAA AGGCACTTCT GACTGTTGCC TCTAAGAAGA AATCCAGAGC AGGGAAGGGG 
GATGCAGCCA AAGAGGATGA CAGCACCAAA ACTGAGTCAG CCCAGCCTGT TGCCATCAGC 
CTTCTCTTTA AAAGATATGT CTTTGACACG CAGAAGAAAA TGATTCAGTC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019360491.1
CDS	10..1743
Translation	MAVAEEPGLGRLNVNVGVLGHIDSGKTALARALSTASSTAAFDRAPQSRARGITLDLGFSCLCTALPPRLGPGHLQFTLVDCPGHASLIRTIIGGAQIIDLMMLVIDVTKGMQTQSAECLVIGQIACQKMVVVLNKIDLLPEGKRQAAIEKMTKKMQKTLENTKFHGCPIVAVAAKPGGPEAPETVNPLGVSELIEVLKSQAYLPSRDPSGPFLMAVDHCFSIKGQGTVMTGTILTGSVSLGDNVEIPALKVSKKVKSMQMFHTPVTYAMQGDRVGICVTQFDPKLLERGLICTPESLQTIHAAIISLKKIQYFRGALHTKAKFHITVGHETVMGRVMFFSPAPADFNKEPLDNAFDFEKDYLYQEEYLSKDSKASDENKDNDQTGEQLPKQQWALVEFEKPVTCPRLCLVIGSKLDTDIHANTCRLAFHGVLLQGLEDKNYTETFLPKLKVYKLKHKEGQVERVIDDYSVIGRSLFKKESNIQIFVGLKVKLSTGEDGIIEGGFGQSGKFKIRIPDGLKPDTKALLTVASKKKSRAGKGDAAKEDDSTKTESAQPVAISLLFKRYVFDTQKKMIQS

Target ORF information:

RefSeq Version	XM_019504946.1
Organism	Gavialis gangeticus(Gharial)
Definition	Gavialis gangeticus eukaryotic elongation factor, selenocysteine-tRNA specific (EEFSEC), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019504946.1

ORF Insert Sequence:

ATGGCGGTGG CTGAGGAGCC GGGGCTGGGG CGGCTGAACG TGAATGTGGG CGTCCTGGGC 
CACATCGACA GCGGCAAGAC GGCGCTGGCC CGAGCGCTCA GCACCGCCAG CTCCACCGCC 
GCCTTCGACC GCGCGCCGCA GAGCCGCGCC CGCGGCATCA CCCTGGACCT GGGCTTCTCG 
TGCCTCTGCA CCGCGCTGCC CCCGCGCCTC GGCCCCGGCC ATCTGCAGTT CACGCTGGTG 
GACTGCCCGG GCCACGCCTC CCTCATCCGG ACCATCATCG GCGGTGCCCA GATTATTGAT 
TTGATGATGC TTGTAATAGA TGTGACAAAA GGGATGCAGA CTCAGTCAGC AGAGTGCTTG 
GTGATTGGAC AAATTGCTTG CCAGAAGATG GTAGTAGTTC TGAACAAAAT AGATCTCCTT 
CCAGAGGGGA AGAGACAGGC TGCCATTGAG AAGATGACCA AGAAAATGCA AAAGACCTTG 
GAAAATACAA AGTTCCATGG GTGTCCTATT GTTGCAGTGG CAGCTAAACC AGGTGGACCA 
GAAGCCCCAG AGACTGTGAA TCCACTAGGT GTTTCTGAAT TAATTGAGGT CCTGAAGTCT 
CAGGCTTACC TGCCATCAAG AGATCCCTCT GGACCCTTCC TTATGGCAGT TGATCACTGT 
TTCTCTATCA AGGGTCAAGG CACAGTGATG ACAGGGACTA TTCTTACTGG CTCTGTTAGC 
CTAGGTGATA ATGTGGAGAT TCCCGCCCTG AAGGTGTCAA AGAAAGTCAA GTCCATGCAG 
ATGTTCCATA CTCCTGTGAC TTACGCCATG CAGGGGGACA GGGTAGGCAT CTGTGTGACC 
CAGTTTGATC CCAAGCTGCT AGAACGGGGC CTCATCTGTA CCCCAGAATC TCTCCAGACC 
ATACATGCTG CCATTATCTC CCTGAAGAAA ATTCAGTATT TTCGAGGTGC GCTGCACACC 
AAAGCCAAGT TTCATATCAC AGTTGGTCAC GAAACAGTTA TGGGACGAGT AATGTTCTTC 
AGTCCAGCCC CTGCTGACTT CAACAAAGAG CCCCTGGACA ACGCGTTTGA TTTTGAAAAA 
GATTACCTTT ATCAAGAAGA ATATTTGTCC AAGGATTCAA AGGCTTCTGA TGAAAACAAG 
GATAATGACC AGACAGGAGA GCAGCTCCCG AAGCAACAGT GGGCCTTAGT GGAGTTTGAA 
AAACCAGTCA CTTGTCCTCG ACTCTGTCTT GTAATTGGGT CAAAACTGGA TACTGATATT 
CATGCAAACA CCTGCAGGCT GGCATTCCAT GGGGTACTGC TTCAAGGCCT AGAAGATAAA 
AACTATACAG AGACTTTCCT TCCAAAGCTG AAAGTGTACA AACTGAAGCA CAAAGAAGGT 
CAAGTGGAAA GGGTGATAGA TGATTACAGC GTGATCGGTC GTTCGCTGTT TAAAAAGGAA 
AGCAACATCC AGATTTTTGT GGGGCTGAAA GTCAAGCTAT CTACAGGAGA GGATGGCATA 
ATAGAGGGTG GCTTTGGGCA GAGTGGTAAA TTTAAAATCC GAATCCCAGA TGGGCTCAAG 
CCAGACACAA AGGCACTTCT GACTGTTGCC TCTAAGAAGA AATCCAGAGC AGGGAAGGGG 
GATGCAGCCA AAGAGGATGA CAGCACCAAA ACTGAGTCAG CCCAGCCTGT TGCCATCAGC 
CTTCTCTTTA AAAGATATGT CTTTGACACG CAGAAGAAAA TGATTCAGTC TTGA