SMIM15 cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	SMIM15
Entrez Gene ID	770642
Full Name	small integral membrane protein 15
Synonyms	C5orf43,CZH5ORF43
General protein information	Preferred Names small integral membrane protein 15 Names small integral membrane protein 15 UPF0542 protein C5orf43 homolog
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001199231.2	NP_001186160.1	small integral membrane protein 15
XM_015277370.2	XP_015132856.1	small integral membrane protein 15 isoform X1
XM_015277369.1	XP_015132855.1	small integral membrane protein 15 isoform X1
XM_015277370.1	XP_015132856.1	small integral membrane protein 15 isoform X1
XM_015277369.2	XP_015132855.1	small integral membrane protein 15 isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	SMIM15	XP_003310765.1
	SMIM15	XP_001082332.2
Bos taurus (cattle)	C7H5orf43	XP_002689367.1
Mus musculus (house mouse)	Smim15	NP_001041715.1
Rattus norvegicus (Norway rat)	Smim15	NP_001264138.1
Homo sapiens (human)	SMIM15	NP_001041714.1
Gallus gallus (chicken)	CZH5ORF43	NP_001186160.1
Canis lupus familiaris (dog)	SMIM15	XP_003433858.2
Danio rerio (zebrafish)	smim15	NP_001153418.1

Gene Ontology
Bibliography

Related articles in PubMed

Chicken genomics resource: sequencing and annotation of 35,407 ESTs from single and multiple tissue cDNA libraries and CAP3 assembly of a chicken gene index.
Carre W, Wang X, Porter TE, Nys Y, Tang J, Bernberg E, Morgan R, Burnside J, Aggrey SE, Simon J, Cogburn LA
Physiological genomics25(3)514-24(2006 May)

Analysis of chicken embryonic gonad expressed sequenced tags.
Shin JH, Kim H, Lim D, Jeon M, Han BK, Park TS, Kim JK, Lillehoj HS, Cho BW, Han JY
Animal genetics37(1)85-6(2006 Feb)

Full-length cDNAs from chicken bursal lymphocytes to facilitate gene function analysis.
Caldwell RB, Kierzek AM, Arakawa H, Bezzubov Y, Zaim J, Fiedler P, Kutter S, Blagodatski A, Kostovska D, Koter M, Plachy J, Carninci P, Hayashizaki Y, Buerstedde JM
Genome biology6(1)R6(2005)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following SMIM15 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SMIM15 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OGa28218	NM_001199231.2 Latest version!	Gallus gallus small integral membrane protein 15 (SMIM15), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OGa28218	XM_015277370.2 Latest version!	Gallus gallus small integral membrane protein 15 (SMIM15), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OGa28218	XM_015277369.2 Latest version!	Gallus gallus small integral membrane protein 15 (SMIM15), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
View Old Accession Versions >>
OGa28218	XM_015277370.1	Gallus gallus small integral membrane protein 15 (SMIM15), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OGa28218	XM_015277369.1	Gallus gallus small integral membrane protein 15 (SMIM15), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SMIM15 ( NM_001199231.2 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_001186160.1 Gallus gallus small integral membrane protein 15 (SMIM15), mRNA.

Price & Availability↑

CloneID	OGa28218
Clone ID Related Accession (Same CDS sequence)	NM_001199231.2 , XM_015277370.2 , XM_015277369.1 , XM_015277370.1 , XM_015277369.2
Accession Version	NM_001199231.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 225bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-09-22
Organism	Gallus gallus(chicken)
Product	small integral membrane protein 15
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AJ453243.1, AC188450.3, BI394192.1 and CV853891.1. On Jan 12, 2012 this sequence version replaced NM_001199231.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: ERR2368565.137733.1, AJ453243.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2201375, SAMEA2201377 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTTGACG TGAAGGCTTG GGCTGTGTAC ATTGTGGAAT GGGCTGCCAA GGACCCCTAT 
GGCTTCCTCA CTACGGTGAT CCTGGTCCTC ACGCCCCTGT TCATCATCAG CGCGGCACTG 
TCGTGGAAGC TTGCAAAGAT GATTGAGACC AGGGAGCGAG AGCAGAAGAA GAAACGGAAA 
CGCCAGGAGA ACATTGTGAA GGCCAAACGA GCAAAGAAGG ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001186160.1
CDS	141..365
Translation	MFDVKAWAVYIVEWAAKDPYGFLTTVILVLTPLFIISAALSWKLAKMIETREREQKKKRKRQENIVKAKRAKKD

Target ORF information:

RefSeq Version	NM_001199231.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus small integral membrane protein 15 (SMIM15), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001199231.2

ORF Insert Sequence:

ATGTTTGACG TGAAGGCTTG GGCTGTGTAC ATTGTGGAAT GGGCTGCCAA GGACCCCTAT 
GGCTTCCTCA CTACGGTGAT CCTGGTCCTC ACGCCCCTGT TCATCATCAG CGCGGCACTG 
TCGTGGAAGC TTGCAAAGAT GATTGAGACC AGGGAGCGAG AGCAGAAGAA GAAACGGAAA 
CGCCAGGAGA ACATTGTGAA GGCCAAACGA GCAAAGAAGG ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMIM15 ( XM_015277370.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015132856.1 Gallus gallus small integral membrane protein 15 (SMIM15), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OGa28218
Clone ID Related Accession (Same CDS sequence)	NM_001199231.2 , XM_015277370.2 , XM_015277369.1 , XM_015277370.1 , XM_015277369.2
Accession Version	XM_015277370.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 225bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	small integral membrane protein 15 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006127.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015277370.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTGACG TGAAGGCTTG GGCTGTGTAC ATTGTGGAAT GGGCTGCCAA GGACCCCTAT 
GGCTTCCTCA CTACGGTGAT CCTGGTCCTC ACGCCCCTGT TCATCATCAG CGCGGCACTG 
TCGTGGAAGC TTGCAAAGAT GATTGAGACC AGGGAGCGAG AGCAGAAGAA GAAACGGAAA 
CGCCAGGAGA ACATTGTGAA GGCCAAACGA GCAAAGAAGG ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015132856.1
CDS	109..333
Translation	MFDVKAWAVYIVEWAAKDPYGFLTTVILVLTPLFIISAALSWKLAKMIETREREQKKKRKRQENIVKAKRAKKD

Target ORF information:

RefSeq Version	XM_015277370.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus small integral membrane protein 15 (SMIM15), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015277370.2

ORF Insert Sequence:

ATGTTTGACG TGAAGGCTTG GGCTGTGTAC ATTGTGGAAT GGGCTGCCAA GGACCCCTAT 
GGCTTCCTCA CTACGGTGAT CCTGGTCCTC ACGCCCCTGT TCATCATCAG CGCGGCACTG 
TCGTGGAAGC TTGCAAAGAT GATTGAGACC AGGGAGCGAG AGCAGAAGAA GAAACGGAAA 
CGCCAGGAGA ACATTGTGAA GGCCAAACGA GCAAAGAAGG ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMIM15 ( XM_015277369.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015132855.1 Gallus gallus small integral membrane protein 15 (SMIM15), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OGa28218
Clone ID Related Accession (Same CDS sequence)	NM_001199231.2 , XM_015277370.2 , XM_015277369.1 , XM_015277370.1 , XM_015277369.2
Accession Version	XM_015277369.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 225bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	small integral membrane protein 15 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001488830.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTGACG TGAAGGCTTG GGCTGTGTAC ATTGTGGAAT GGGCTGCCAA GGACCCCTAT 
GGCTTCCTCA CTACGGTGAT CCTGGTCCTC ACGCCCCTGT TCATCATCAG CGCGGCACTG 
TCGTGGAAGC TTGCAAAGAT GATTGAGACC AGGGAGCGAG AGCAGAAGAA GAAACGGAAA 
CGCCAGGAGA ACATTGTGAA GGCCAAACGA GCAAAGAAGG ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015132855.1
CDS	130..354
Translation	MFDVKAWAVYIVEWAAKDPYGFLTTVILVLTPLFIISAALSWKLAKMIETREREQKKKRKRQENIVKAKRAKKD

Target ORF information:

RefSeq Version	XM_015277369.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus small integral membrane protein 15 (SMIM15), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015277369.1

ORF Insert Sequence:

ATGTTTGACG TGAAGGCTTG GGCTGTGTAC ATTGTGGAAT GGGCTGCCAA GGACCCCTAT 
GGCTTCCTCA CTACGGTGAT CCTGGTCCTC ACGCCCCTGT TCATCATCAG CGCGGCACTG 
TCGTGGAAGC TTGCAAAGAT GATTGAGACC AGGGAGCGAG AGCAGAAGAA GAAACGGAAA 
CGCCAGGAGA ACATTGTGAA GGCCAAACGA GCAAAGAAGG ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMIM15 ( XM_015277370.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015132856.1 Gallus gallus small integral membrane protein 15 (SMIM15), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OGa28218
Clone ID Related Accession (Same CDS sequence)	NM_001199231.2 , XM_015277370.2 , XM_015277369.1 , XM_015277370.1 , XM_015277369.2
Accession Version	XM_015277370.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 225bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	small integral membrane protein 15 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001488830.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTGACG TGAAGGCTTG GGCTGTGTAC ATTGTGGAAT GGGCTGCCAA GGACCCCTAT 
GGCTTCCTCA CTACGGTGAT CCTGGTCCTC ACGCCCCTGT TCATCATCAG CGCGGCACTG 
TCGTGGAAGC TTGCAAAGAT GATTGAGACC AGGGAGCGAG AGCAGAAGAA GAAACGGAAA 
CGCCAGGAGA ACATTGTGAA GGCCAAACGA GCAAAGAAGG ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015132856.1
CDS	109..333
Translation	MFDVKAWAVYIVEWAAKDPYGFLTTVILVLTPLFIISAALSWKLAKMIETREREQKKKRKRQENIVKAKRAKKD

Target ORF information:

RefSeq Version	XM_015277370.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus small integral membrane protein 15 (SMIM15), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015277370.1

ORF Insert Sequence:

ATGTTTGACG TGAAGGCTTG GGCTGTGTAC ATTGTGGAAT GGGCTGCCAA GGACCCCTAT 
GGCTTCCTCA CTACGGTGAT CCTGGTCCTC ACGCCCCTGT TCATCATCAG CGCGGCACTG 
TCGTGGAAGC TTGCAAAGAT GATTGAGACC AGGGAGCGAG AGCAGAAGAA GAAACGGAAA 
CGCCAGGAGA ACATTGTGAA GGCCAAACGA GCAAAGAAGG ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMIM15 ( XM_015277369.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015132855.1 Gallus gallus small integral membrane protein 15 (SMIM15), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OGa28218
Clone ID Related Accession (Same CDS sequence)	NM_001199231.2 , XM_015277370.2 , XM_015277369.1 , XM_015277370.1 , XM_015277369.2
Accession Version	XM_015277369.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 225bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	small integral membrane protein 15 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006127.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015277369.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTGACG TGAAGGCTTG GGCTGTGTAC ATTGTGGAAT GGGCTGCCAA GGACCCCTAT 
GGCTTCCTCA CTACGGTGAT CCTGGTCCTC ACGCCCCTGT TCATCATCAG CGCGGCACTG 
TCGTGGAAGC TTGCAAAGAT GATTGAGACC AGGGAGCGAG AGCAGAAGAA GAAACGGAAA 
CGCCAGGAGA ACATTGTGAA GGCCAAACGA GCAAAGAAGG ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015132855.1
CDS	129..353
Translation	MFDVKAWAVYIVEWAAKDPYGFLTTVILVLTPLFIISAALSWKLAKMIETREREQKKKRKRQENIVKAKRAKKD

Target ORF information:

RefSeq Version	XM_015277369.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus small integral membrane protein 15 (SMIM15), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015277369.2

ORF Insert Sequence:

ATGTTTGACG TGAAGGCTTG GGCTGTGTAC ATTGTGGAAT GGGCTGCCAA GGACCCCTAT 
GGCTTCCTCA CTACGGTGAT CCTGGTCCTC ACGCCCCTGT TCATCATCAG CGCGGCACTG 
TCGTGGAAGC TTGCAAAGAT GATTGAGACC AGGGAGCGAG AGCAGAAGAA GAAACGGAAA 
CGCCAGGAGA ACATTGTGAA GGCCAAACGA GCAAAGAAGG ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Chicken genomics resource: sequencing and annotation of 35,407 ESTs from single and multiple tissue cDNA libraries and CAP3 assembly of a chicken gene index.
Physiological genomics25(3)514-24(2006 May)
Carre W,Wang X,Porter TE,Nys Y,Tang J,Bernberg E,Morgan R,Burnside J,Aggrey SE,Simon J,Cogburn LA

Analysis of chicken embryonic gonad expressed sequenced tags.
Animal genetics37(1)85-6(2006 Feb)
Shin JH,Kim H,Lim D,Jeon M,Han BK,Park TS,Kim JK,Lillehoj HS,Cho BW,Han JY

Full-length cDNAs from chicken bursal lymphocytes to facilitate gene function analysis.
Genome biology6(1)R6(2005)
Caldwell RB,Kierzek AM,Arakawa H,Bezzubov Y,Zaim J,Fiedler P,Kutter S,Blagodatski A,Kostovska D,Koter M,Plachy J,Carninci P,Hayashizaki Y,Buerstedde JM

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SMIM15 cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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