TLR5 cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	TLR5
Entrez Gene ID	554217
Full Name	toll like receptor 5
General protein information	Preferred Names toll like receptor 5 Names toll-like receptor 5
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001024586.1	NP_001019757.1	toll-like receptor 5 precursor
XM_015283741.1	XP_015139227.1	toll-like receptor 5 isoform X1
XM_025148815.1	XP_025004583.1	toll-like receptor 5 isoform X1
XM_015283741.2	XP_015139227.1	toll-like receptor 5 isoform X1
XM_015283742.2	XP_015139228.1	toll-like receptor 5 isoform X1
XM_015283740.1	XP_015139226.1	toll-like receptor 5 isoform X1
XM_015283742.1	XP_015139228.1	toll-like receptor 5 isoform X1

Pathways from BioSystems

Homologies

	TLR5	NP_001123901.1
Canis lupus familiaris (dog)	TLR5	NP_001184105.1
Gallus gallus (chicken)	TLR5	NP_001019757.1
Danio rerio (zebrafish)	tlr5b	NP_001124067.1
Xenopus tropicalis (tropical clawed frog)	LOC100496647	XP_002940742.2
Danio rerio (zebrafish)	tlr5a	XP_001919052.2
Homo sapiens (human)	TLR5	NP_003259.2
Bos taurus (cattle)	TLR5	NP_001035591.1
Pan troglodytes (chimpanzee)	TLR5	NP_001123934.1
Mus musculus (house mouse)	Tlr5	NP_058624.2
Rattus norvegicus (Norway rat)	Tlr5	NP_001139300.1

Gene Ontology
Bibliography

Related articles in PubMed

Treatment with ligands for toll-like receptors 2 and 5 induces a mixed T-helper 1- and 2-like response in chicken splenocytes.
St Paul M, Paolucci S, Sharif S
Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research32(12)592-8(2012 Dec)

Different genetic patterns in avian Toll-like receptor (TLR)5 genes.
Ruan W, Wu Y, Zheng SJ
Molecular biology reports39(4)3419-26(2012 Apr)

Toll-like receptor mRNA expression, iNOS gene polymorphism and serum nitric oxide levels in indigenous chickens.
Ramasamy KT, Reddy MR, Murugesan S
Veterinary research communications35(5)321-7(2011 Jun)

Functional characterization of chicken TLR5 reveals species-specific recognition of flagellin.
Keestra AM, de Zoete MR, van Aubel RA, van Putten JP
Molecular immunology45(5)1298-307(2008 Mar)

Identification and functional characterization of chicken toll-like receptor 5 reveals a fundamental role in the biology of infection with Salmonella enterica serovar typhimurium.
Iqbal M, Philbin VJ, Withanage GS, Wigley P, Beal RK, Goodchild MJ, Barrow P, McConnell I, Maskell DJ, Young J, Bumstead N, Boyd Y, Smith AL
Infection and immunity73(4)2344-50(2005 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data suggest that activation of TLR5 induces mixed TH1-/TH2-like response in chicken splenocytes; expression of specific cytokines is up-regulated (TH1-associated interferon-gamma and interleukin-12; TH2-associated interleukin-4 and interleukin-13).
Title: Treatment with ligands for toll-like receptors 2 and 5 induces a mixed T-helper 1- and 2-like response in chicken splenocytes.

Avian TLR5 genes are polymorphic among avian breeds.
Title: Different genetic patterns in avian Toll-like receptor (TLR)5 genes.

Kadaknath breed expressed significantly (P < 0.01) higher levels of TLR4 transcripts than Aseel breed, however there is no significant difference in TLR5 mRNA expression between these two breeds.
Title: Toll-like receptor mRNA expression, iNOS gene polymorphism and serum nitric oxide levels in indigenous chickens.

chickens express a functional TLR5 albeit with different flagellin sensing qualities compared to human TLR5; single amino acid substitutions in bacterial flagellin can alter the species-specific TLR5 response
Title: Functional characterization of chicken TLR5 reveals species-specific recognition of flagellin.

The following TLR5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TLR5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OGa01089	NM_001024586.1 Latest version!	Gallus gallus toll like receptor 5 (TLR5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OGa33403	XM_015283741.2 Latest version!	Gallus gallus toll like receptor 5 (TLR5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OGa33403	XM_015283742.2 Latest version!	Gallus gallus toll like receptor 5 (TLR5), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OGa33403	XM_015283740.1 Latest version!	Gallus gallus toll-like receptor 5 (TLR5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OGa33403	XM_025148815.1 Latest version!	Gallus gallus toll like receptor 5 (TLR5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
View Old Accession Versions >>
OGa33403	XM_015283741.1	Gallus gallus toll-like receptor 5 (TLR5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OGa33403	XM_015283742.1	Gallus gallus toll-like receptor 5 (TLR5), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TLR5 ( NM_001024586.1 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_001019757.1 Gallus gallus toll like receptor 5 (TLR5), mRNA.

Price & Availability↑

CloneID	OGa01089
Clone ID Related Accession (Same CDS sequence)	NM_001024586.1
Accession Version	NM_001024586.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2586bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-11-05
Organism	Gallus gallus(chicken)
Product	toll-like receptor 5 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DQ004555.1. ##Evidence-Data-START## Transcript exon combination :: DQ004555.1 [ECO:0000332] ##Evidence-Data-END##

ATGATGTTAC ATCAACGGCT AATAATTGTC TTTGGAATAG CACTAGCTGG AGATATATGT 
GCATCTAGAA GCTGCTATTC AGAAGATCAG GTCTCCATGT ATAATTCTTG CAACCTCACA 
GGTGTTCCAC CTGTGCCAAA GGACACTGCA AAGCTTTTTC TAACTTACAA CTATATCAGA 
CAAGTGACTG CAACTTCATT TCCACTGCTG GAGGATTTGT TCTTGTTGGA AATTGGAACA 
CAACGCGTCT TTCCTCTTTA CATAGGAAAA GAAGCTTTCA GGAACCTGCC AAATCTTCGT 
GTCTTGGACC TGGGGTTCAA TAACATTCTT CTGCTCGATC TTGATTCTTT TGCGGGTTTG 
CAACGTTTGA CCATACTCCG TCTGTTTCAG AACAACCTTG GAGATTCCAT CCTGGAGGAG 
CGTTACTTTC AAGACTTGAG ATCATTAGAA GAATTGGATC TTTCAGGAAA CCAAATCACA 
AAACTTCATC CACACCCCTT ATTTTATAAT CTAACCATCT TGAAAGCTGT GAACCTGAAA 
TTCAACAAGA TATCCAACCT GTGTGAAAGT AATCTTACCA GCTTCCAAGG AAAACACTTT 
TCATTCTTTA GCCTCAGTAC TAACACTCTG TATAAGACAG ATAAAATGAT CTGGGCCAAA 
TGCCCAAATC CTTTCAGAAA TATTACATTT AACTCACTGG ACGTTAGTGA GAATGGCTGG 
AGCACAGAGA CAGTCCAGTA CTTCTGCACA GCTATTAAGG GGACTCAAAT CAATTATTTA 
TCATTTCGCT CTCATACAAT GGGCTCAGGA TTTGGCTTTA ATAACTTAAA AAATCCAGAT 
ACTGATACAT TTACAGGACT AGCAAGAAGT GATCTTCATT TGCTTGATAT TTCCAATGGA 
TTCATTTTCT CTCTCAATTC TTTAATCTTT GAAAGTCTTC GTAATCTGGA ATTTCTAAAC 
CTTTTCAGAA ACAAGATAAA TCAAATCCAA AAGCAAGCAT TTTTTGGCTT GGAAAACCTA 
GAAATTCTCA ATCTCTCAAG TAACCTTTTA GGTGAGTTGT ACGATTATAC TTTTGAGGGG 
CTACACAGTA TAATGTATAT TGATTTACAG CAAAATCATA TTGGGATGAT TGGTGAAAAA 
TCTTTCAGTA ACTTAGTAAA TCTGAAAATA ATTGATCTCC GAGACAATGC CATTAAAAAA 
CTCCCTTCCT TCCCACATCT GACTTCTGCC TTTTTAAGTG ACAATAAGAT GATGTCTGTA 
GCTCACACGG CAATAGTAGC AACACATATT GAATTAGAGA GAAACTGGCT CGCAAACCTG 
GGTGACCTGT ATGTTCTTTT CCAAGTTCCA GGTGTGCAGT ATCTCCTCTT AAAACAGAAT 
CGCTTCTCTT ACTGTGTGAA ACATGTTGAT GCTATAGAAA ATAACCAGTT GATTTATATG 
GACCTAGGAG AAAACATGTT GCAGCTTGTG TGGGAGAGAG GTTTATGTTT GGATGTGTTC 
AGGACACTGT CTAAACTTCA GGTTCTACAC CTGAACAACA ACTACCTTAG TGCTCTTCCA 
CAGGAGATTT TTAATGGTCT AACATCTCTA AAAAGACTTA ATCTAGCTTC CAACCTATTG 
TCTCACCTCT CTCTCAGGGT TTTTCCACAA AGCCTAACAA ACCTAAACTT GTCTGGAAAT 
CAGCTTTTTT CCCCTAAGCC TGAAGTCTTT ATGACTTTGA GTATTCTGGA TATAACACAT 
AACAAGTATG TCTGTGACTG TGCTTTAAAA AGCCTATTAG TGTGGCTAAA TGAAACCAAT 
GTAACCCTAG CTGGCTCGGA GTCTGACAGG TACTGTGTGT ACCCACCTGC ATTAGCGGGT 
GTACCCGTGT CATTTCTGAC ATACGATGAC TGCGATGAAG ATGAACTCCA GCAGACACTC 
AGGTTCTCGG TATTCGTTTT CCTATCTGTC ACCCTTCTGA TGTTTCTGAT GTCAACCATT 
ATTTTTACTC GCTGTCGGGG CATTTGTTTT GTCTGGTATA AGACCATCAC CAAAACTTTG 
ATAGGAAGCC ATCCACCTGC AGCAGATACA AGTGAATACA TGTACGACGC ATATTTGTGC 
TACAGCAAAA ATGATTTCGA GTGGGTCCAG AATTCCTTGC TGAAGCACCT GGATTCACAA 
TATTTTGATA AAAACAGATT TACCTTGTGC TTTGAGGAAC GAGATTTCTT GCCTGGGGAA 
GAACATATCA ACAATATTCG TGATGCTATT TGGAAGAGCA GGAAAACAAT TTGTGTTGTG 
ACCAGGCAGT TTCTCAAAGA TGGGTGGTGT GTAGAAGCCT TTAATTTTGC CCAGAGCAGG 
TACTTCAGTG ATTTGAAAGA GGTTCTCATT ATGGTAGTGG TTGGGTCACT TTCTCAATAT 
CAACTGATGA AACACAAACC AATTCGAATT TTTTTACAAA GGAGTCGATA TCTGCGTTGG 
CCTGAAGATT ATCAAGATAT AGGCTGGTTT TTGGATAACC TTTCTTCCCA AATTCTGAAG 
GAAAAAAAAG TGCAAAGGAA CGTCAGTGGT ATAGAGCTGC AAACTATAGC GACAGTCTCA 
CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001019757.1
CDS	1..2586
Translation	MMLHQRLIIVFGIALAGDICASRSCYSEDQVSMYNSCNLTGVPPVPKDTAKLFLTYNYIRQVTATSFPLLEDLFLLEIGTQRVFPLYIGKEAFRNLPNLRVLDLGFNNILLLDLDSFAGLQRLTILRLFQNNLGDSILEERYFQDLRSLEELDLSGNQITKLHPHPLFYNLTILKAVNLKFNKISNLCESNLTSFQGKHFSFFSLSTNTLYKTDKMIWAKCPNPFRNITFNSLDVSENGWSTETVQYFCTAIKGTQINYLSFRSHTMGSGFGFNNLKNPDTDTFTGLARSDLHLLDISNGFIFSLNSLIFESLRNLEFLNLFRNKINQIQKQAFFGLENLEILNLSSNLLGELYDYTFEGLHSIMYIDLQQNHIGMIGEKSFSNLVNLKIIDLRDNAIKKLPSFPHLTSAFLSDNKMMSVAHTAIVATHIELERNWLANLGDLYVLFQVPGVQYLLLKQNRFSYCVKHVDAIENNQLIYMDLGENMLQLVWERGLCLDVFRTLSKLQVLHLNNNYLSALPQEIFNGLTSLKRLNLASNLLSHLSLRVFPQSLTNLNLSGNQLFSPKPEVFMTLSILDITHNKYVCDCALKSLLVWLNETNVTLAGSESDRYCVYPPALAGVPVSFLTYDDCDEDELQQTLRFSVFVFLSVTLLMFLMSTIIFTRCRGICFVWYKTITKTLIGSHPPAADTSEYMYDAYLCYSKNDFEWVQNSLLKHLDSQYFDKNRFTLCFEERDFLPGEEHINNIRDAIWKSRKTICVVTRQFLKDGWCVEAFNFAQSRYFSDLKEVLIMVVVGSLSQYQLMKHKPIRIFLQRSRYLRWPEDYQDIGWFLDNLSSQILKEKKVQRNVSGIELQTIATVSH

Target ORF information:

RefSeq Version	NM_001024586.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus toll like receptor 5 (TLR5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001024586.1

ORF Insert Sequence:

ATGATGTTAC ATCAACGGCT AATAATTGTC TTTGGAATAG CACTAGCTGG AGATATATGT 
GCATCTAGAA GCTGCTATTC AGAAGATCAG GTCTCCATGT ATAATTCTTG CAACCTCACA 
GGTGTTCCAC CTGTGCCAAA GGACACTGCA AAGCTTTTTC TAACTTACAA CTATATCAGA 
CAAGTGACTG CAACTTCATT TCCACTGCTG GAGGATTTGT TCTTGTTGGA AATTGGAACA 
CAACGCGTCT TTCCTCTTTA CATAGGAAAA GAAGCTTTCA GGAACCTGCC AAATCTTCGT 
GTCTTGGACC TGGGGTTCAA TAACATTCTT CTGCTCGATC TTGATTCTTT TGCGGGTTTG 
CAACGTTTGA CCATACTCCG TCTGTTTCAG AACAACCTTG GAGATTCCAT CCTGGAGGAG 
CGTTACTTTC AAGACTTGAG ATCATTAGAA GAATTGGATC TTTCAGGAAA CCAAATCACA 
AAACTTCATC CACACCCCTT ATTTTATAAT CTAACCATCT TGAAAGCTGT GAACCTGAAA 
TTCAACAAGA TATCCAACCT GTGTGAAAGT AATCTTACCA GCTTCCAAGG AAAACACTTT 
TCATTCTTTA GCCTCAGTAC TAACACTCTG TATAAGACAG ATAAAATGAT CTGGGCCAAA 
TGCCCAAATC CTTTCAGAAA TATTACATTT AACTCACTGG ACGTTAGTGA GAATGGCTGG 
AGCACAGAGA CAGTCCAGTA CTTCTGCACA GCTATTAAGG GGACTCAAAT CAATTATTTA 
TCATTTCGCT CTCATACAAT GGGCTCAGGA TTTGGCTTTA ATAACTTAAA AAATCCAGAT 
ACTGATACAT TTACAGGACT AGCAAGAAGT GATCTTCATT TGCTTGATAT TTCCAATGGA 
TTCATTTTCT CTCTCAATTC TTTAATCTTT GAAAGTCTTC GTAATCTGGA ATTTCTAAAC 
CTTTTCAGAA ACAAGATAAA TCAAATCCAA AAGCAAGCAT TTTTTGGCTT GGAAAACCTA 
GAAATTCTCA ATCTCTCAAG TAACCTTTTA GGTGAGTTGT ACGATTATAC TTTTGAGGGG 
CTACACAGTA TAATGTATAT TGATTTACAG CAAAATCATA TTGGGATGAT TGGTGAAAAA 
TCTTTCAGTA ACTTAGTAAA TCTGAAAATA ATTGATCTCC GAGACAATGC CATTAAAAAA 
CTCCCTTCCT TCCCACATCT GACTTCTGCC TTTTTAAGTG ACAATAAGAT GATGTCTGTA 
GCTCACACGG CAATAGTAGC AACACATATT GAATTAGAGA GAAACTGGCT CGCAAACCTG 
GGTGACCTGT ATGTTCTTTT CCAAGTTCCA GGTGTGCAGT ATCTCCTCTT AAAACAGAAT 
CGCTTCTCTT ACTGTGTGAA ACATGTTGAT GCTATAGAAA ATAACCAGTT GATTTATATG 
GACCTAGGAG AAAACATGTT GCAGCTTGTG TGGGAGAGAG GTTTATGTTT GGATGTGTTC 
AGGACACTGT CTAAACTTCA GGTTCTACAC CTGAACAACA ACTACCTTAG TGCTCTTCCA 
CAGGAGATTT TTAATGGTCT AACATCTCTA AAAAGACTTA ATCTAGCTTC CAACCTATTG 
TCTCACCTCT CTCTCAGGGT TTTTCCACAA AGCCTAACAA ACCTAAACTT GTCTGGAAAT 
CAGCTTTTTT CCCCTAAGCC TGAAGTCTTT ATGACTTTGA GTATTCTGGA TATAACACAT 
AACAAGTATG TCTGTGACTG TGCTTTAAAA AGCCTATTAG TGTGGCTAAA TGAAACCAAT 
GTAACCCTAG CTGGCTCGGA GTCTGACAGG TACTGTGTGT ACCCACCTGC ATTAGCGGGT 
GTACCCGTGT CATTTCTGAC ATACGATGAC TGCGATGAAG ATGAACTCCA GCAGACACTC 
AGGTTCTCGG TATTCGTTTT CCTATCTGTC ACCCTTCTGA TGTTTCTGAT GTCAACCATT 
ATTTTTACTC GCTGTCGGGG CATTTGTTTT GTCTGGTATA AGACCATCAC CAAAACTTTG 
ATAGGAAGCC ATCCACCTGC AGCAGATACA AGTGAATACA TGTACGACGC ATATTTGTGC 
TACAGCAAAA ATGATTTCGA GTGGGTCCAG AATTCCTTGC TGAAGCACCT GGATTCACAA 
TATTTTGATA AAAACAGATT TACCTTGTGC TTTGAGGAAC GAGATTTCTT GCCTGGGGAA 
GAACATATCA ACAATATTCG TGATGCTATT TGGAAGAGCA GGAAAACAAT TTGTGTTGTG 
ACCAGGCAGT TTCTCAAAGA TGGGTGGTGT GTAGAAGCCT TTAATTTTGC CCAGAGCAGG 
TACTTCAGTG ATTTGAAAGA GGTTCTCATT ATGGTAGTGG TTGGGTCACT TTCTCAATAT 
CAACTGATGA AACACAAACC AATTCGAATT TTTTTACAAA GGAGTCGATA TCTGCGTTGG 
CCTGAAGATT ATCAAGATAT AGGCTGGTTT TTGGATAACC TTTCTTCCCA AATTCTGAAG 
GAAAAAAAAG TGCAAAGGAA CGTCAGTGGT ATAGAGCTGC AAACTATAGC GACAGTCTCA 
CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TLR5 ( XM_015283741.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015139227.1 Gallus gallus toll-like receptor 5 (TLR5), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OGa33403
Clone ID Related Accession (Same CDS sequence)	XM_015283741.1 , XM_025148815.1 , XM_015283741.2 , XM_015283742.2 , XM_015283740.1 , XM_015283742.1
Accession Version	XM_015283741.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2586bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	toll-like receptor 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455833.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTTAC ATCAACGGCT AATAATTGTC TTTGGAATAG CACTAGCTGG AGATATATGT 
GCATCTAGAA GCTGCTATTC AGAAGATCAG GTCTCCATGT ATAATTCTTG CAACCTCACA 
GGTGTTCCAC CTGTGCCAAA GGACACTGCA AAGCTTTTTC TAACTTACAA CTATATCAGA 
CAAGTGACTG CAACTTCATT TCCACTGCTG GAGGATTTGT TCTTGTTGGA AATTGGAACA 
CAACGCGTCT TTCCTCTTTA CATAGGAAAA GAAGCTTTCA GGAACCTGCC AAATCTTCGT 
GTCTTGGACC TGGGGTTCAA TAACATTCTT CTGCTGGATC TTGATTCTTT TGCGGGTTTG 
CAACGTTTGA CCATACTCCG TCTGTTTCAG AACAACCTTG GAGATTCCAT CCTGGAGGAG 
CGTTACTTTC AAGACTTGAG ATCATTAGAA GAATTGGATC TTTCAGGAAA CCAAATCACA 
AAACTTCATC CACACCCCTT ATTTTATAAT CTAACCATCT TGAAAGCTGT GAACCTGAAA 
TTCAACAAGA TATCCAACCT GTGTGAAAGT AATCTTACCA GCTTCCAAGG AAAACACTTT 
TCATTCTTTA GCCTCAGTAC TAACACTCTG TATAGGACAG ATAAAATGAT CTGGGCCAAA 
TGCCCAAATC CTTTCAGAAA TATTACATTT AACTCACTGG ACGTTAGTGA GAATGGCTGG 
AGCACAGAGA CAGTCCAGTA CTTCTGCACA GCTATTAAGG GGACTCAAAT CAATTATTTA 
TCATTTCGCT CTCATACAAT GGGCTCAGGA TTTGGCTTTA ATAACTTAAA AAATCCAGAT 
ACTGATACAT TTACAGGACT AGCAAGAAGT GATCTTCATT TGCTTGATAT TTCCAATGGA 
TTCATTTTCT CTCTCAATTC TTTAATCTTT GAAAGTCTTC GTAATCTGGA ATTTCTAAAC 
CTTTTCAGAA ACAAGATAAA TCAAATCCAA AAGCAAGCAT TTTTTGGCTT GGAAAACCTA 
GAAATTCTCA ATCTCTCAAG TAACCTTTTA GGTGAGTTGT ACGATTATAC TTTTGAGGGG 
CTACACAGTA TAATGTATAT TGATTTACAG CAAAATCATA TTGGGATGAT TGGTGAAAAA 
TCTTTCAGTA ACTTAGTAAA TCTGAAAATA ATTGATCTCC GAGACAATGC CATTAAAAAA 
CTCCCTTCCT TCCCACATCT GACTTCTGCC TTTTTAAGTG ACAATAAGCT GATGTCTGTA 
GCTCACACGG CAATAGTAGC AACACATATT GAATTAGAGA GAAACTGGCT CGCAAACCTG 
GGTGACCTGT ATGTTCTTTT CCAAGTTCCA GGTGTGCAGT ATCTCCTCTT AAAACAGAAT 
CGCTTCTCTT ACTGTGTGAA ACATGTTGAT GCTATAGAAA ATAACCAGTT GATTTATATG 
GACCTAGGAG AAAACATGTT GCAGCTTGTG TGGGAGAGAG GTTTATGTTT GGATGTGTTC 
AGGACACTGT CTAAACTTCA GGTTCTACAC CTGAACAACA ACTACCTTAG TGCTCTTCCA 
CAGGAGATTT TTAATGGTCT AACATCTCTA AAAAGACTTA ATCTAGCTTC CAACCTATTG 
TCTCACCTCT CTCTCAGGGT TTTTCCACAA AGCCTAATAA ACCTAAACTT GTCTGGAAAT 
CAGCTTTTTT CCCCTAAGCC TGAAGTCTTT ATGACTTTGA GTATTCTGGA TATAACACAT 
AACAAGTATG TCTGTGACTG TGCTTTAAAA AGCCTATTAG TGTGGCTAAA TGAAACCAAT 
GTAACCCTAG CTGGCTCGGA GTCTGACAGG TACTGTGTGT ACCCACCTGC ATTAGCGGGT 
GTACCCGTGT CATTTCTGAC ATACGATGAC TGCGATGAAG ATGAACTCCA GCAGACACTC 
AGGTTCTCGG TATTCGTTTT CCTATCTGTC ACCCTTCTGA TGTTTCTGAT GTCAACCATT 
ATTTTTACTC GCTGTCGGGG CATTTGTTTT GTCTGGTATA AGACCATCAC CAAAACTTTG 
ATAGGAAGCC ATCCACCTGC AGCAGATACA AGTGAATACA TGTACGACGC ATATTTGTGC 
TACAGCAAAA ATGATTTCGA GTGGGTCCAG AATTCCTTGC TGAAGCACCT GGATTCACAA 
TATTTTGATA AAAACAGATT TACCTTGTGC TTTGAGGAAC GAGATTTCTT GCCTGGGGAA 
GAACATATCA ACAATATTCG TGATGCTATT TGGAAGAGCA GGAAAACAAT TTGTGTTGTG 
ACCAGGCAGT TTCTCAAAGA TGGGTGGTGT GTAGAAGCCT TTAATTTTGC CCAGAGCAGG 
TACTTCAGTG ATTTGAAAGA GGTTCTCATT ATGGTAGTGG TTGGGTCACT TTCTCAATAT 
CAACTGATGA AACACAAACC AATTCGAATT TTTTTACAAA GGAGTCGATA TCTGCGTTGG 
CCTGAAGATT ATCAAGATAT AGGCTGGTTT TTGGATAACC TTTCTTCACA AATTCTGAAG 
GAAAAAAAAG TGCAAAGGAA CGTCAGTGGT ATAGAGCTGC AAACTATAGC GACAGTCTCA 
CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015139227.1
CDS	3694..6279
Translation	MMLHQRLIIVFGIALAGDICASRSCYSEDQVSMYNSCNLTGVPPVPKDTAKLFLTYNYIRQVTATSFPLLEDLFLLEIGTQRVFPLYIGKEAFRNLPNLRVLDLGFNNILLLDLDSFAGLQRLTILRLFQNNLGDSILEERYFQDLRSLEELDLSGNQITKLHPHPLFYNLTILKAVNLKFNKISNLCESNLTSFQGKHFSFFSLSTNTLYRTDKMIWAKCPNPFRNITFNSLDVSENGWSTETVQYFCTAIKGTQINYLSFRSHTMGSGFGFNNLKNPDTDTFTGLARSDLHLLDISNGFIFSLNSLIFESLRNLEFLNLFRNKINQIQKQAFFGLENLEILNLSSNLLGELYDYTFEGLHSIMYIDLQQNHIGMIGEKSFSNLVNLKIIDLRDNAIKKLPSFPHLTSAFLSDNKLMSVAHTAIVATHIELERNWLANLGDLYVLFQVPGVQYLLLKQNRFSYCVKHVDAIENNQLIYMDLGENMLQLVWERGLCLDVFRTLSKLQVLHLNNNYLSALPQEIFNGLTSLKRLNLASNLLSHLSLRVFPQSLINLNLSGNQLFSPKPEVFMTLSILDITHNKYVCDCALKSLLVWLNETNVTLAGSESDRYCVYPPALAGVPVSFLTYDDCDEDELQQTLRFSVFVFLSVTLLMFLMSTIIFTRCRGICFVWYKTITKTLIGSHPPAADTSEYMYDAYLCYSKNDFEWVQNSLLKHLDSQYFDKNRFTLCFEERDFLPGEEHINNIRDAIWKSRKTICVVTRQFLKDGWCVEAFNFAQSRYFSDLKEVLIMVVVGSLSQYQLMKHKPIRIFLQRSRYLRWPEDYQDIGWFLDNLSSQILKEKKVQRNVSGIELQTIATVSH

Target ORF information:

RefSeq Version	XM_015283741.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus toll-like receptor 5 (TLR5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015283741.1

ORF Insert Sequence:

ATGATGTTAC ATCAACGGCT AATAATTGTC TTTGGAATAG CACTAGCTGG AGATATATGT 
GCATCTAGAA GCTGCTATTC AGAAGATCAG GTCTCCATGT ATAATTCTTG CAACCTCACA 
GGTGTTCCAC CTGTGCCAAA GGACACTGCA AAGCTTTTTC TAACTTACAA CTATATCAGA 
CAAGTGACTG CAACTTCATT TCCACTGCTG GAGGATTTGT TCTTGTTGGA AATTGGAACA 
CAACGCGTCT TTCCTCTTTA CATAGGAAAA GAAGCTTTCA GGAACCTGCC AAATCTTCGT 
GTCTTGGACC TGGGGTTCAA TAACATTCTT CTGCTGGATC TTGATTCTTT TGCGGGTTTG 
CAACGTTTGA CCATACTCCG TCTGTTTCAG AACAACCTTG GAGATTCCAT CCTGGAGGAG 
CGTTACTTTC AAGACTTGAG ATCATTAGAA GAATTGGATC TTTCAGGAAA CCAAATCACA 
AAACTTCATC CACACCCCTT ATTTTATAAT CTAACCATCT TGAAAGCTGT GAACCTGAAA 
TTCAACAAGA TATCCAACCT GTGTGAAAGT AATCTTACCA GCTTCCAAGG AAAACACTTT 
TCATTCTTTA GCCTCAGTAC TAACACTCTG TATAGGACAG ATAAAATGAT CTGGGCCAAA 
TGCCCAAATC CTTTCAGAAA TATTACATTT AACTCACTGG ACGTTAGTGA GAATGGCTGG 
AGCACAGAGA CAGTCCAGTA CTTCTGCACA GCTATTAAGG GGACTCAAAT CAATTATTTA 
TCATTTCGCT CTCATACAAT GGGCTCAGGA TTTGGCTTTA ATAACTTAAA AAATCCAGAT 
ACTGATACAT TTACAGGACT AGCAAGAAGT GATCTTCATT TGCTTGATAT TTCCAATGGA 
TTCATTTTCT CTCTCAATTC TTTAATCTTT GAAAGTCTTC GTAATCTGGA ATTTCTAAAC 
CTTTTCAGAA ACAAGATAAA TCAAATCCAA AAGCAAGCAT TTTTTGGCTT GGAAAACCTA 
GAAATTCTCA ATCTCTCAAG TAACCTTTTA GGTGAGTTGT ACGATTATAC TTTTGAGGGG 
CTACACAGTA TAATGTATAT TGATTTACAG CAAAATCATA TTGGGATGAT TGGTGAAAAA 
TCTTTCAGTA ACTTAGTAAA TCTGAAAATA ATTGATCTCC GAGACAATGC CATTAAAAAA 
CTCCCTTCCT TCCCACATCT GACTTCTGCC TTTTTAAGTG ACAATAAGCT GATGTCTGTA 
GCTCACACGG CAATAGTAGC AACACATATT GAATTAGAGA GAAACTGGCT CGCAAACCTG 
GGTGACCTGT ATGTTCTTTT CCAAGTTCCA GGTGTGCAGT ATCTCCTCTT AAAACAGAAT 
CGCTTCTCTT ACTGTGTGAA ACATGTTGAT GCTATAGAAA ATAACCAGTT GATTTATATG 
GACCTAGGAG AAAACATGTT GCAGCTTGTG TGGGAGAGAG GTTTATGTTT GGATGTGTTC 
AGGACACTGT CTAAACTTCA GGTTCTACAC CTGAACAACA ACTACCTTAG TGCTCTTCCA 
CAGGAGATTT TTAATGGTCT AACATCTCTA AAAAGACTTA ATCTAGCTTC CAACCTATTG 
TCTCACCTCT CTCTCAGGGT TTTTCCACAA AGCCTAATAA ACCTAAACTT GTCTGGAAAT 
CAGCTTTTTT CCCCTAAGCC TGAAGTCTTT ATGACTTTGA GTATTCTGGA TATAACACAT 
AACAAGTATG TCTGTGACTG TGCTTTAAAA AGCCTATTAG TGTGGCTAAA TGAAACCAAT 
GTAACCCTAG CTGGCTCGGA GTCTGACAGG TACTGTGTGT ACCCACCTGC ATTAGCGGGT 
GTACCCGTGT CATTTCTGAC ATACGATGAC TGCGATGAAG ATGAACTCCA GCAGACACTC 
AGGTTCTCGG TATTCGTTTT CCTATCTGTC ACCCTTCTGA TGTTTCTGAT GTCAACCATT 
ATTTTTACTC GCTGTCGGGG CATTTGTTTT GTCTGGTATA AGACCATCAC CAAAACTTTG 
ATAGGAAGCC ATCCACCTGC AGCAGATACA AGTGAATACA TGTACGACGC ATATTTGTGC 
TACAGCAAAA ATGATTTCGA GTGGGTCCAG AATTCCTTGC TGAAGCACCT GGATTCACAA 
TATTTTGATA AAAACAGATT TACCTTGTGC TTTGAGGAAC GAGATTTCTT GCCTGGGGAA 
GAACATATCA ACAATATTCG TGATGCTATT TGGAAGAGCA GGAAAACAAT TTGTGTTGTG 
ACCAGGCAGT TTCTCAAAGA TGGGTGGTGT GTAGAAGCCT TTAATTTTGC CCAGAGCAGG 
TACTTCAGTG ATTTGAAAGA GGTTCTCATT ATGGTAGTGG TTGGGTCACT TTCTCAATAT 
CAACTGATGA AACACAAACC AATTCGAATT TTTTTACAAA GGAGTCGATA TCTGCGTTGG 
CCTGAAGATT ATCAAGATAT AGGCTGGTTT TTGGATAACC TTTCTTCACA AATTCTGAAG 
GAAAAAAAAG TGCAAAGGAA CGTCAGTGGT ATAGAGCTGC AAACTATAGC GACAGTCTCA 
CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TLR5 ( XM_025148815.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025004583.1 Gallus gallus toll like receptor 5 (TLR5), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OGa33403
Clone ID Related Accession (Same CDS sequence)	XM_015283741.1 , XM_025148815.1 , XM_015283741.2 , XM_015283742.2 , XM_015283740.1 , XM_015283742.1
Accession Version	XM_025148815.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2586bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	toll-like receptor 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006090.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTTAC ATCAACGGCT AATAATTGTC TTTGGAATAG CACTAGCTGG AGATATATGT 
GCATCTAGAA GCTGCTATTC AGAAGATCAG GTCTCCATGT ATAATTCTTG CAACCTCACA 
GGTGTTCCAC CTGTGCCAAA GGACACTGCA AAGCTTTTTC TAACTTACAA CTATATCAGA 
CAAGTGACTG CAACTTCATT TCCACTGCTG GAGGATTTGT TCTTGTTGGA AATTGGAACA 
CAACGCGTCT TTCCTCTTTA CATAGGAAAA GAAGCTTTCA GGAACCTGCC AAATCTTCGT 
GTCTTGGACC TGGGGTTCAA TAACATTCTT CTGCTGGATC TTGATTCTTT TGCGGGTTTG 
CAACGTTTGA CCATACTCCG TCTGTTTCAG AACAACCTTG GAGATTCCAT CCTGGAGGAG 
CGTTACTTTC AAGACTTGAG ATCATTAGAA GAATTGGATC TTTCAGGAAA CCAAATCACA 
AAACTTCATC CACACCCCTT ATTTTATAAT CTAACCATCT TGAAAGCTGT GAACCTGAAA 
TTCAACAAGA TATCCAACCT GTGTGAAAGT AATCTTACCA GCTTCCAAGG AAAACACTTT 
TCATTCTTTA GCCTCAGTAC TAACACTCTG TATAGGACAG ATAAAATGAT CTGGGCCAAA 
TGCCCAAATC CTTTCAGAAA TATTACATTT AACTCACTGG ACGTTAGTGA GAATGGCTGG 
AGCACAGAGA CAGTCCAGTA CTTCTGCACA GCTATTAAGG GGACTCAAAT CAATTATTTA 
TCATTTCGCT CTCATACAAT GGGCTCAGGA TTTGGCTTTA ATAACTTAAA AAATCCAGAT 
ACTGATACAT TTACAGGACT AGCAAGAAGT GATCTTCATT TGCTTGATAT TTCCAATGGA 
TTCATTTTCT CTCTCAATTC TTTAATCTTT GAAAGTCTTC GTAATCTGGA ATTTCTAAAC 
CTTTTCAGAA ACAAGATAAA TCAAATCCAA AAGCAAGCAT TTTTTGGCTT GGAAAACCTA 
GAAATTCTCA ATCTCTCAAG TAACCTTTTA GGTGAGTTGT ACGATTATAC TTTTGAGGGG 
CTACACAGTA TAATGTATAT TGATTTACAG CAAAATCATA TTGGGATGAT TGGTGAAAAA 
TCTTTCAGTA ACTTAGTAAA TCTGAAAATA ATTGATCTCC GAGACAATGC CATTAAAAAA 
CTCCCTTCCT TCCCACATCT GACTTCTGCC TTTTTAAGTG ACAATAAGCT GATGTCTGTA 
GCTCACACGG CAATAGTAGC AACACATATT GAATTAGAGA GAAACTGGCT CGCAAACCTG 
GGTGACCTGT ATGTTCTTTT CCAAGTTCCA GGTGTGCAGT ATCTCCTCTT AAAACAGAAT 
CGCTTCTCTT ACTGTGTGAA ACATGTTGAT GCTATAGAAA ATAACCAGTT GATTTATATG 
GACCTAGGAG AAAACATGTT GCAGCTTGTG TGGGAGAGAG GTTTATGTTT GGATGTGTTC 
AGGACACTGT CTAAACTTCA GGTTCTACAC CTGAACAACA ACTACCTTAG TGCTCTTCCA 
CAGGAGATTT TTAATGGTCT AACATCTCTA AAAAGACTTA ATCTAGCTTC CAACCTATTG 
TCTCACCTCT CTCTCAGGGT TTTTCCACAA AGCCTAATAA ACCTAAACTT GTCTGGAAAT 
CAGCTTTTTT CCCCTAAGCC TGAAGTCTTT ATGACTTTGA GTATTCTGGA TATAACACAT 
AACAAGTATG TCTGTGACTG TGCTTTAAAA AGCCTATTAG TGTGGCTAAA TGAAACCAAT 
GTAACCCTAG CTGGCTCGGA GTCTGACAGG TACTGTGTGT ACCCACCTGC ATTAGCGGGT 
GTACCCGTGT CATTTCTGAC ATACGATGAC TGCGATGAAG ATGAACTCCA GCAGACACTC 
AGGTTCTCGG TATTCGTTTT CCTATCTGTC ACCCTTCTGA TGTTTCTGAT GTCAACCATT 
ATTTTTACTC GCTGTCGGGG CATTTGTTTT GTCTGGTATA AGACCATCAC CAAAACTTTG 
ATAGGAAGCC ATCCACCTGC AGCAGATACA AGTGAATACA TGTACGACGC ATATTTGTGC 
TACAGCAAAA ATGATTTCGA GTGGGTCCAG AATTCCTTGC TGAAGCACCT GGATTCACAA 
TATTTTGATA AAAACAGATT TACCTTGTGC TTTGAGGAAC GAGATTTCTT GCCTGGGGAA 
GAACATATCA ACAATATTCG TGATGCTATT TGGAAGAGCA GGAAAACAAT TTGTGTTGTG 
ACCAGGCAGT TTCTCAAAGA TGGGTGGTGT GTAGAAGCCT TTAATTTTGC CCAGAGCAGG 
TACTTCAGTG ATTTGAAAGA GGTTCTCATT ATGGTAGTGG TTGGGTCACT TTCTCAATAT 
CAACTGATGA AACACAAACC AATTCGAATT TTTTTACAAA GGAGTCGATA TCTGCGTTGG 
CCTGAAGATT ATCAAGATAT AGGCTGGTTT TTGGATAACC TTTCTTCACA AATTCTGAAG 
GAAAAAAAAG TGCAAAGGAA CGTCAGTGGT ATAGAGCTGC AAACTATAGC GACAGTCTCA 
CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025004583.1
CDS	6587..9172
Translation	MMLHQRLIIVFGIALAGDICASRSCYSEDQVSMYNSCNLTGVPPVPKDTAKLFLTYNYIRQVTATSFPLLEDLFLLEIGTQRVFPLYIGKEAFRNLPNLRVLDLGFNNILLLDLDSFAGLQRLTILRLFQNNLGDSILEERYFQDLRSLEELDLSGNQITKLHPHPLFYNLTILKAVNLKFNKISNLCESNLTSFQGKHFSFFSLSTNTLYRTDKMIWAKCPNPFRNITFNSLDVSENGWSTETVQYFCTAIKGTQINYLSFRSHTMGSGFGFNNLKNPDTDTFTGLARSDLHLLDISNGFIFSLNSLIFESLRNLEFLNLFRNKINQIQKQAFFGLENLEILNLSSNLLGELYDYTFEGLHSIMYIDLQQNHIGMIGEKSFSNLVNLKIIDLRDNAIKKLPSFPHLTSAFLSDNKLMSVAHTAIVATHIELERNWLANLGDLYVLFQVPGVQYLLLKQNRFSYCVKHVDAIENNQLIYMDLGENMLQLVWERGLCLDVFRTLSKLQVLHLNNNYLSALPQEIFNGLTSLKRLNLASNLLSHLSLRVFPQSLINLNLSGNQLFSPKPEVFMTLSILDITHNKYVCDCALKSLLVWLNETNVTLAGSESDRYCVYPPALAGVPVSFLTYDDCDEDELQQTLRFSVFVFLSVTLLMFLMSTIIFTRCRGICFVWYKTITKTLIGSHPPAADTSEYMYDAYLCYSKNDFEWVQNSLLKHLDSQYFDKNRFTLCFEERDFLPGEEHINNIRDAIWKSRKTICVVTRQFLKDGWCVEAFNFAQSRYFSDLKEVLIMVVVGSLSQYQLMKHKPIRIFLQRSRYLRWPEDYQDIGWFLDNLSSQILKEKKVQRNVSGIELQTIATVSH

Target ORF information:

RefSeq Version	XM_025148815.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus toll like receptor 5 (TLR5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025148815.1

ORF Insert Sequence:

ATGATGTTAC ATCAACGGCT AATAATTGTC TTTGGAATAG CACTAGCTGG AGATATATGT 
GCATCTAGAA GCTGCTATTC AGAAGATCAG GTCTCCATGT ATAATTCTTG CAACCTCACA 
GGTGTTCCAC CTGTGCCAAA GGACACTGCA AAGCTTTTTC TAACTTACAA CTATATCAGA 
CAAGTGACTG CAACTTCATT TCCACTGCTG GAGGATTTGT TCTTGTTGGA AATTGGAACA 
CAACGCGTCT TTCCTCTTTA CATAGGAAAA GAAGCTTTCA GGAACCTGCC AAATCTTCGT 
GTCTTGGACC TGGGGTTCAA TAACATTCTT CTGCTGGATC TTGATTCTTT TGCGGGTTTG 
CAACGTTTGA CCATACTCCG TCTGTTTCAG AACAACCTTG GAGATTCCAT CCTGGAGGAG 
CGTTACTTTC AAGACTTGAG ATCATTAGAA GAATTGGATC TTTCAGGAAA CCAAATCACA 
AAACTTCATC CACACCCCTT ATTTTATAAT CTAACCATCT TGAAAGCTGT GAACCTGAAA 
TTCAACAAGA TATCCAACCT GTGTGAAAGT AATCTTACCA GCTTCCAAGG AAAACACTTT 
TCATTCTTTA GCCTCAGTAC TAACACTCTG TATAGGACAG ATAAAATGAT CTGGGCCAAA 
TGCCCAAATC CTTTCAGAAA TATTACATTT AACTCACTGG ACGTTAGTGA GAATGGCTGG 
AGCACAGAGA CAGTCCAGTA CTTCTGCACA GCTATTAAGG GGACTCAAAT CAATTATTTA 
TCATTTCGCT CTCATACAAT GGGCTCAGGA TTTGGCTTTA ATAACTTAAA AAATCCAGAT 
ACTGATACAT TTACAGGACT AGCAAGAAGT GATCTTCATT TGCTTGATAT TTCCAATGGA 
TTCATTTTCT CTCTCAATTC TTTAATCTTT GAAAGTCTTC GTAATCTGGA ATTTCTAAAC 
CTTTTCAGAA ACAAGATAAA TCAAATCCAA AAGCAAGCAT TTTTTGGCTT GGAAAACCTA 
GAAATTCTCA ATCTCTCAAG TAACCTTTTA GGTGAGTTGT ACGATTATAC TTTTGAGGGG 
CTACACAGTA TAATGTATAT TGATTTACAG CAAAATCATA TTGGGATGAT TGGTGAAAAA 
TCTTTCAGTA ACTTAGTAAA TCTGAAAATA ATTGATCTCC GAGACAATGC CATTAAAAAA 
CTCCCTTCCT TCCCACATCT GACTTCTGCC TTTTTAAGTG ACAATAAGCT GATGTCTGTA 
GCTCACACGG CAATAGTAGC AACACATATT GAATTAGAGA GAAACTGGCT CGCAAACCTG 
GGTGACCTGT ATGTTCTTTT CCAAGTTCCA GGTGTGCAGT ATCTCCTCTT AAAACAGAAT 
CGCTTCTCTT ACTGTGTGAA ACATGTTGAT GCTATAGAAA ATAACCAGTT GATTTATATG 
GACCTAGGAG AAAACATGTT GCAGCTTGTG TGGGAGAGAG GTTTATGTTT GGATGTGTTC 
AGGACACTGT CTAAACTTCA GGTTCTACAC CTGAACAACA ACTACCTTAG TGCTCTTCCA 
CAGGAGATTT TTAATGGTCT AACATCTCTA AAAAGACTTA ATCTAGCTTC CAACCTATTG 
TCTCACCTCT CTCTCAGGGT TTTTCCACAA AGCCTAATAA ACCTAAACTT GTCTGGAAAT 
CAGCTTTTTT CCCCTAAGCC TGAAGTCTTT ATGACTTTGA GTATTCTGGA TATAACACAT 
AACAAGTATG TCTGTGACTG TGCTTTAAAA AGCCTATTAG TGTGGCTAAA TGAAACCAAT 
GTAACCCTAG CTGGCTCGGA GTCTGACAGG TACTGTGTGT ACCCACCTGC ATTAGCGGGT 
GTACCCGTGT CATTTCTGAC ATACGATGAC TGCGATGAAG ATGAACTCCA GCAGACACTC 
AGGTTCTCGG TATTCGTTTT CCTATCTGTC ACCCTTCTGA TGTTTCTGAT GTCAACCATT 
ATTTTTACTC GCTGTCGGGG CATTTGTTTT GTCTGGTATA AGACCATCAC CAAAACTTTG 
ATAGGAAGCC ATCCACCTGC AGCAGATACA AGTGAATACA TGTACGACGC ATATTTGTGC 
TACAGCAAAA ATGATTTCGA GTGGGTCCAG AATTCCTTGC TGAAGCACCT GGATTCACAA 
TATTTTGATA AAAACAGATT TACCTTGTGC TTTGAGGAAC GAGATTTCTT GCCTGGGGAA 
GAACATATCA ACAATATTCG TGATGCTATT TGGAAGAGCA GGAAAACAAT TTGTGTTGTG 
ACCAGGCAGT TTCTCAAAGA TGGGTGGTGT GTAGAAGCCT TTAATTTTGC CCAGAGCAGG 
TACTTCAGTG ATTTGAAAGA GGTTCTCATT ATGGTAGTGG TTGGGTCACT TTCTCAATAT 
CAACTGATGA AACACAAACC AATTCGAATT TTTTTACAAA GGAGTCGATA TCTGCGTTGG 
CCTGAAGATT ATCAAGATAT AGGCTGGTTT TTGGATAACC TTTCTTCACA AATTCTGAAG 
GAAAAAAAAG TGCAAAGGAA CGTCAGTGGT ATAGAGCTGC AAACTATAGC GACAGTCTCA 
CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TLR5 ( XM_015283741.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015139227.1 Gallus gallus toll like receptor 5 (TLR5), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OGa33403
Clone ID Related Accession (Same CDS sequence)	XM_015283741.1 , XM_025148815.1 , XM_015283741.2 , XM_015283742.2 , XM_015283740.1 , XM_015283742.1
Accession Version	XM_015283741.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2586bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	toll-like receptor 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006090.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015283741.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTTAC ATCAACGGCT AATAATTGTC TTTGGAATAG CACTAGCTGG AGATATATGT 
GCATCTAGAA GCTGCTATTC AGAAGATCAG GTCTCCATGT ATAATTCTTG CAACCTCACA 
GGTGTTCCAC CTGTGCCAAA GGACACTGCA AAGCTTTTTC TAACTTACAA CTATATCAGA 
CAAGTGACTG CAACTTCATT TCCACTGCTG GAGGATTTGT TCTTGTTGGA AATTGGAACA 
CAACGCGTCT TTCCTCTTTA CATAGGAAAA GAAGCTTTCA GGAACCTGCC AAATCTTCGT 
GTCTTGGACC TGGGGTTCAA TAACATTCTT CTGCTGGATC TTGATTCTTT TGCGGGTTTG 
CAACGTTTGA CCATACTCCG TCTGTTTCAG AACAACCTTG GAGATTCCAT CCTGGAGGAG 
CGTTACTTTC AAGACTTGAG ATCATTAGAA GAATTGGATC TTTCAGGAAA CCAAATCACA 
AAACTTCATC CACACCCCTT ATTTTATAAT CTAACCATCT TGAAAGCTGT GAACCTGAAA 
TTCAACAAGA TATCCAACCT GTGTGAAAGT AATCTTACCA GCTTCCAAGG AAAACACTTT 
TCATTCTTTA GCCTCAGTAC TAACACTCTG TATAGGACAG ATAAAATGAT CTGGGCCAAA 
TGCCCAAATC CTTTCAGAAA TATTACATTT AACTCACTGG ACGTTAGTGA GAATGGCTGG 
AGCACAGAGA CAGTCCAGTA CTTCTGCACA GCTATTAAGG GGACTCAAAT CAATTATTTA 
TCATTTCGCT CTCATACAAT GGGCTCAGGA TTTGGCTTTA ATAACTTAAA AAATCCAGAT 
ACTGATACAT TTACAGGACT AGCAAGAAGT GATCTTCATT TGCTTGATAT TTCCAATGGA 
TTCATTTTCT CTCTCAATTC TTTAATCTTT GAAAGTCTTC GTAATCTGGA ATTTCTAAAC 
CTTTTCAGAA ACAAGATAAA TCAAATCCAA AAGCAAGCAT TTTTTGGCTT GGAAAACCTA 
GAAATTCTCA ATCTCTCAAG TAACCTTTTA GGTGAGTTGT ACGATTATAC TTTTGAGGGG 
CTACACAGTA TAATGTATAT TGATTTACAG CAAAATCATA TTGGGATGAT TGGTGAAAAA 
TCTTTCAGTA ACTTAGTAAA TCTGAAAATA ATTGATCTCC GAGACAATGC CATTAAAAAA 
CTCCCTTCCT TCCCACATCT GACTTCTGCC TTTTTAAGTG ACAATAAGCT GATGTCTGTA 
GCTCACACGG CAATAGTAGC AACACATATT GAATTAGAGA GAAACTGGCT CGCAAACCTG 
GGTGACCTGT ATGTTCTTTT CCAAGTTCCA GGTGTGCAGT ATCTCCTCTT AAAACAGAAT 
CGCTTCTCTT ACTGTGTGAA ACATGTTGAT GCTATAGAAA ATAACCAGTT GATTTATATG 
GACCTAGGAG AAAACATGTT GCAGCTTGTG TGGGAGAGAG GTTTATGTTT GGATGTGTTC 
AGGACACTGT CTAAACTTCA GGTTCTACAC CTGAACAACA ACTACCTTAG TGCTCTTCCA 
CAGGAGATTT TTAATGGTCT AACATCTCTA AAAAGACTTA ATCTAGCTTC CAACCTATTG 
TCTCACCTCT CTCTCAGGGT TTTTCCACAA AGCCTAATAA ACCTAAACTT GTCTGGAAAT 
CAGCTTTTTT CCCCTAAGCC TGAAGTCTTT ATGACTTTGA GTATTCTGGA TATAACACAT 
AACAAGTATG TCTGTGACTG TGCTTTAAAA AGCCTATTAG TGTGGCTAAA TGAAACCAAT 
GTAACCCTAG CTGGCTCGGA GTCTGACAGG TACTGTGTGT ACCCACCTGC ATTAGCGGGT 
GTACCCGTGT CATTTCTGAC ATACGATGAC TGCGATGAAG ATGAACTCCA GCAGACACTC 
AGGTTCTCGG TATTCGTTTT CCTATCTGTC ACCCTTCTGA TGTTTCTGAT GTCAACCATT 
ATTTTTACTC GCTGTCGGGG CATTTGTTTT GTCTGGTATA AGACCATCAC CAAAACTTTG 
ATAGGAAGCC ATCCACCTGC AGCAGATACA AGTGAATACA TGTACGACGC ATATTTGTGC 
TACAGCAAAA ATGATTTCGA GTGGGTCCAG AATTCCTTGC TGAAGCACCT GGATTCACAA 
TATTTTGATA AAAACAGATT TACCTTGTGC TTTGAGGAAC GAGATTTCTT GCCTGGGGAA 
GAACATATCA ACAATATTCG TGATGCTATT TGGAAGAGCA GGAAAACAAT TTGTGTTGTG 
ACCAGGCAGT TTCTCAAAGA TGGGTGGTGT GTAGAAGCCT TTAATTTTGC CCAGAGCAGG 
TACTTCAGTG ATTTGAAAGA GGTTCTCATT ATGGTAGTGG TTGGGTCACT TTCTCAATAT 
CAACTGATGA AACACAAACC AATTCGAATT TTTTTACAAA GGAGTCGATA TCTGCGTTGG 
CCTGAAGATT ATCAAGATAT AGGCTGGTTT TTGGATAACC TTTCTTCACA AATTCTGAAG 
GAAAAAAAAG TGCAAAGGAA CGTCAGTGGT ATAGAGCTGC AAACTATAGC GACAGTCTCA 
CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015139227.1
CDS	3863..6448
Translation	MMLHQRLIIVFGIALAGDICASRSCYSEDQVSMYNSCNLTGVPPVPKDTAKLFLTYNYIRQVTATSFPLLEDLFLLEIGTQRVFPLYIGKEAFRNLPNLRVLDLGFNNILLLDLDSFAGLQRLTILRLFQNNLGDSILEERYFQDLRSLEELDLSGNQITKLHPHPLFYNLTILKAVNLKFNKISNLCESNLTSFQGKHFSFFSLSTNTLYRTDKMIWAKCPNPFRNITFNSLDVSENGWSTETVQYFCTAIKGTQINYLSFRSHTMGSGFGFNNLKNPDTDTFTGLARSDLHLLDISNGFIFSLNSLIFESLRNLEFLNLFRNKINQIQKQAFFGLENLEILNLSSNLLGELYDYTFEGLHSIMYIDLQQNHIGMIGEKSFSNLVNLKIIDLRDNAIKKLPSFPHLTSAFLSDNKLMSVAHTAIVATHIELERNWLANLGDLYVLFQVPGVQYLLLKQNRFSYCVKHVDAIENNQLIYMDLGENMLQLVWERGLCLDVFRTLSKLQVLHLNNNYLSALPQEIFNGLTSLKRLNLASNLLSHLSLRVFPQSLINLNLSGNQLFSPKPEVFMTLSILDITHNKYVCDCALKSLLVWLNETNVTLAGSESDRYCVYPPALAGVPVSFLTYDDCDEDELQQTLRFSVFVFLSVTLLMFLMSTIIFTRCRGICFVWYKTITKTLIGSHPPAADTSEYMYDAYLCYSKNDFEWVQNSLLKHLDSQYFDKNRFTLCFEERDFLPGEEHINNIRDAIWKSRKTICVVTRQFLKDGWCVEAFNFAQSRYFSDLKEVLIMVVVGSLSQYQLMKHKPIRIFLQRSRYLRWPEDYQDIGWFLDNLSSQILKEKKVQRNVSGIELQTIATVSH

Target ORF information:

RefSeq Version	XM_015283741.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus toll like receptor 5 (TLR5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015283741.2

ORF Insert Sequence:

ATGATGTTAC ATCAACGGCT AATAATTGTC TTTGGAATAG CACTAGCTGG AGATATATGT 
GCATCTAGAA GCTGCTATTC AGAAGATCAG GTCTCCATGT ATAATTCTTG CAACCTCACA 
GGTGTTCCAC CTGTGCCAAA GGACACTGCA AAGCTTTTTC TAACTTACAA CTATATCAGA 
CAAGTGACTG CAACTTCATT TCCACTGCTG GAGGATTTGT TCTTGTTGGA AATTGGAACA 
CAACGCGTCT TTCCTCTTTA CATAGGAAAA GAAGCTTTCA GGAACCTGCC AAATCTTCGT 
GTCTTGGACC TGGGGTTCAA TAACATTCTT CTGCTGGATC TTGATTCTTT TGCGGGTTTG 
CAACGTTTGA CCATACTCCG TCTGTTTCAG AACAACCTTG GAGATTCCAT CCTGGAGGAG 
CGTTACTTTC AAGACTTGAG ATCATTAGAA GAATTGGATC TTTCAGGAAA CCAAATCACA 
AAACTTCATC CACACCCCTT ATTTTATAAT CTAACCATCT TGAAAGCTGT GAACCTGAAA 
TTCAACAAGA TATCCAACCT GTGTGAAAGT AATCTTACCA GCTTCCAAGG AAAACACTTT 
TCATTCTTTA GCCTCAGTAC TAACACTCTG TATAGGACAG ATAAAATGAT CTGGGCCAAA 
TGCCCAAATC CTTTCAGAAA TATTACATTT AACTCACTGG ACGTTAGTGA GAATGGCTGG 
AGCACAGAGA CAGTCCAGTA CTTCTGCACA GCTATTAAGG GGACTCAAAT CAATTATTTA 
TCATTTCGCT CTCATACAAT GGGCTCAGGA TTTGGCTTTA ATAACTTAAA AAATCCAGAT 
ACTGATACAT TTACAGGACT AGCAAGAAGT GATCTTCATT TGCTTGATAT TTCCAATGGA 
TTCATTTTCT CTCTCAATTC TTTAATCTTT GAAAGTCTTC GTAATCTGGA ATTTCTAAAC 
CTTTTCAGAA ACAAGATAAA TCAAATCCAA AAGCAAGCAT TTTTTGGCTT GGAAAACCTA 
GAAATTCTCA ATCTCTCAAG TAACCTTTTA GGTGAGTTGT ACGATTATAC TTTTGAGGGG 
CTACACAGTA TAATGTATAT TGATTTACAG CAAAATCATA TTGGGATGAT TGGTGAAAAA 
TCTTTCAGTA ACTTAGTAAA TCTGAAAATA ATTGATCTCC GAGACAATGC CATTAAAAAA 
CTCCCTTCCT TCCCACATCT GACTTCTGCC TTTTTAAGTG ACAATAAGCT GATGTCTGTA 
GCTCACACGG CAATAGTAGC AACACATATT GAATTAGAGA GAAACTGGCT CGCAAACCTG 
GGTGACCTGT ATGTTCTTTT CCAAGTTCCA GGTGTGCAGT ATCTCCTCTT AAAACAGAAT 
CGCTTCTCTT ACTGTGTGAA ACATGTTGAT GCTATAGAAA ATAACCAGTT GATTTATATG 
GACCTAGGAG AAAACATGTT GCAGCTTGTG TGGGAGAGAG GTTTATGTTT GGATGTGTTC 
AGGACACTGT CTAAACTTCA GGTTCTACAC CTGAACAACA ACTACCTTAG TGCTCTTCCA 
CAGGAGATTT TTAATGGTCT AACATCTCTA AAAAGACTTA ATCTAGCTTC CAACCTATTG 
TCTCACCTCT CTCTCAGGGT TTTTCCACAA AGCCTAATAA ACCTAAACTT GTCTGGAAAT 
CAGCTTTTTT CCCCTAAGCC TGAAGTCTTT ATGACTTTGA GTATTCTGGA TATAACACAT 
AACAAGTATG TCTGTGACTG TGCTTTAAAA AGCCTATTAG TGTGGCTAAA TGAAACCAAT 
GTAACCCTAG CTGGCTCGGA GTCTGACAGG TACTGTGTGT ACCCACCTGC ATTAGCGGGT 
GTACCCGTGT CATTTCTGAC ATACGATGAC TGCGATGAAG ATGAACTCCA GCAGACACTC 
AGGTTCTCGG TATTCGTTTT CCTATCTGTC ACCCTTCTGA TGTTTCTGAT GTCAACCATT 
ATTTTTACTC GCTGTCGGGG CATTTGTTTT GTCTGGTATA AGACCATCAC CAAAACTTTG 
ATAGGAAGCC ATCCACCTGC AGCAGATACA AGTGAATACA TGTACGACGC ATATTTGTGC 
TACAGCAAAA ATGATTTCGA GTGGGTCCAG AATTCCTTGC TGAAGCACCT GGATTCACAA 
TATTTTGATA AAAACAGATT TACCTTGTGC TTTGAGGAAC GAGATTTCTT GCCTGGGGAA 
GAACATATCA ACAATATTCG TGATGCTATT TGGAAGAGCA GGAAAACAAT TTGTGTTGTG 
ACCAGGCAGT TTCTCAAAGA TGGGTGGTGT GTAGAAGCCT TTAATTTTGC CCAGAGCAGG 
TACTTCAGTG ATTTGAAAGA GGTTCTCATT ATGGTAGTGG TTGGGTCACT TTCTCAATAT 
CAACTGATGA AACACAAACC AATTCGAATT TTTTTACAAA GGAGTCGATA TCTGCGTTGG 
CCTGAAGATT ATCAAGATAT AGGCTGGTTT TTGGATAACC TTTCTTCACA AATTCTGAAG 
GAAAAAAAAG TGCAAAGGAA CGTCAGTGGT ATAGAGCTGC AAACTATAGC GACAGTCTCA 
CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TLR5 ( XM_015283742.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015139228.1 Gallus gallus toll like receptor 5 (TLR5), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OGa33403
Clone ID Related Accession (Same CDS sequence)	XM_015283741.1 , XM_025148815.1 , XM_015283741.2 , XM_015283742.2 , XM_015283740.1 , XM_015283742.1
Accession Version	XM_015283742.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2586bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	toll-like receptor 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006090.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015283742.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTTAC ATCAACGGCT AATAATTGTC TTTGGAATAG CACTAGCTGG AGATATATGT 
GCATCTAGAA GCTGCTATTC AGAAGATCAG GTCTCCATGT ATAATTCTTG CAACCTCACA 
GGTGTTCCAC CTGTGCCAAA GGACACTGCA AAGCTTTTTC TAACTTACAA CTATATCAGA 
CAAGTGACTG CAACTTCATT TCCACTGCTG GAGGATTTGT TCTTGTTGGA AATTGGAACA 
CAACGCGTCT TTCCTCTTTA CATAGGAAAA GAAGCTTTCA GGAACCTGCC AAATCTTCGT 
GTCTTGGACC TGGGGTTCAA TAACATTCTT CTGCTGGATC TTGATTCTTT TGCGGGTTTG 
CAACGTTTGA CCATACTCCG TCTGTTTCAG AACAACCTTG GAGATTCCAT CCTGGAGGAG 
CGTTACTTTC AAGACTTGAG ATCATTAGAA GAATTGGATC TTTCAGGAAA CCAAATCACA 
AAACTTCATC CACACCCCTT ATTTTATAAT CTAACCATCT TGAAAGCTGT GAACCTGAAA 
TTCAACAAGA TATCCAACCT GTGTGAAAGT AATCTTACCA GCTTCCAAGG AAAACACTTT 
TCATTCTTTA GCCTCAGTAC TAACACTCTG TATAGGACAG ATAAAATGAT CTGGGCCAAA 
TGCCCAAATC CTTTCAGAAA TATTACATTT AACTCACTGG ACGTTAGTGA GAATGGCTGG 
AGCACAGAGA CAGTCCAGTA CTTCTGCACA GCTATTAAGG GGACTCAAAT CAATTATTTA 
TCATTTCGCT CTCATACAAT GGGCTCAGGA TTTGGCTTTA ATAACTTAAA AAATCCAGAT 
ACTGATACAT TTACAGGACT AGCAAGAAGT GATCTTCATT TGCTTGATAT TTCCAATGGA 
TTCATTTTCT CTCTCAATTC TTTAATCTTT GAAAGTCTTC GTAATCTGGA ATTTCTAAAC 
CTTTTCAGAA ACAAGATAAA TCAAATCCAA AAGCAAGCAT TTTTTGGCTT GGAAAACCTA 
GAAATTCTCA ATCTCTCAAG TAACCTTTTA GGTGAGTTGT ACGATTATAC TTTTGAGGGG 
CTACACAGTA TAATGTATAT TGATTTACAG CAAAATCATA TTGGGATGAT TGGTGAAAAA 
TCTTTCAGTA ACTTAGTAAA TCTGAAAATA ATTGATCTCC GAGACAATGC CATTAAAAAA 
CTCCCTTCCT TCCCACATCT GACTTCTGCC TTTTTAAGTG ACAATAAGCT GATGTCTGTA 
GCTCACACGG CAATAGTAGC AACACATATT GAATTAGAGA GAAACTGGCT CGCAAACCTG 
GGTGACCTGT ATGTTCTTTT CCAAGTTCCA GGTGTGCAGT ATCTCCTCTT AAAACAGAAT 
CGCTTCTCTT ACTGTGTGAA ACATGTTGAT GCTATAGAAA ATAACCAGTT GATTTATATG 
GACCTAGGAG AAAACATGTT GCAGCTTGTG TGGGAGAGAG GTTTATGTTT GGATGTGTTC 
AGGACACTGT CTAAACTTCA GGTTCTACAC CTGAACAACA ACTACCTTAG TGCTCTTCCA 
CAGGAGATTT TTAATGGTCT AACATCTCTA AAAAGACTTA ATCTAGCTTC CAACCTATTG 
TCTCACCTCT CTCTCAGGGT TTTTCCACAA AGCCTAATAA ACCTAAACTT GTCTGGAAAT 
CAGCTTTTTT CCCCTAAGCC TGAAGTCTTT ATGACTTTGA GTATTCTGGA TATAACACAT 
AACAAGTATG TCTGTGACTG TGCTTTAAAA AGCCTATTAG TGTGGCTAAA TGAAACCAAT 
GTAACCCTAG CTGGCTCGGA GTCTGACAGG TACTGTGTGT ACCCACCTGC ATTAGCGGGT 
GTACCCGTGT CATTTCTGAC ATACGATGAC TGCGATGAAG ATGAACTCCA GCAGACACTC 
AGGTTCTCGG TATTCGTTTT CCTATCTGTC ACCCTTCTGA TGTTTCTGAT GTCAACCATT 
ATTTTTACTC GCTGTCGGGG CATTTGTTTT GTCTGGTATA AGACCATCAC CAAAACTTTG 
ATAGGAAGCC ATCCACCTGC AGCAGATACA AGTGAATACA TGTACGACGC ATATTTGTGC 
TACAGCAAAA ATGATTTCGA GTGGGTCCAG AATTCCTTGC TGAAGCACCT GGATTCACAA 
TATTTTGATA AAAACAGATT TACCTTGTGC TTTGAGGAAC GAGATTTCTT GCCTGGGGAA 
GAACATATCA ACAATATTCG TGATGCTATT TGGAAGAGCA GGAAAACAAT TTGTGTTGTG 
ACCAGGCAGT TTCTCAAAGA TGGGTGGTGT GTAGAAGCCT TTAATTTTGC CCAGAGCAGG 
TACTTCAGTG ATTTGAAAGA GGTTCTCATT ATGGTAGTGG TTGGGTCACT TTCTCAATAT 
CAACTGATGA AACACAAACC AATTCGAATT TTTTTACAAA GGAGTCGATA TCTGCGTTGG 
CCTGAAGATT ATCAAGATAT AGGCTGGTTT TTGGATAACC TTTCTTCACA AATTCTGAAG 
GAAAAAAAAG TGCAAAGGAA CGTCAGTGGT ATAGAGCTGC AAACTATAGC GACAGTCTCA 
CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015139228.1
CDS	565..3150
Translation	MMLHQRLIIVFGIALAGDICASRSCYSEDQVSMYNSCNLTGVPPVPKDTAKLFLTYNYIRQVTATSFPLLEDLFLLEIGTQRVFPLYIGKEAFRNLPNLRVLDLGFNNILLLDLDSFAGLQRLTILRLFQNNLGDSILEERYFQDLRSLEELDLSGNQITKLHPHPLFYNLTILKAVNLKFNKISNLCESNLTSFQGKHFSFFSLSTNTLYRTDKMIWAKCPNPFRNITFNSLDVSENGWSTETVQYFCTAIKGTQINYLSFRSHTMGSGFGFNNLKNPDTDTFTGLARSDLHLLDISNGFIFSLNSLIFESLRNLEFLNLFRNKINQIQKQAFFGLENLEILNLSSNLLGELYDYTFEGLHSIMYIDLQQNHIGMIGEKSFSNLVNLKIIDLRDNAIKKLPSFPHLTSAFLSDNKLMSVAHTAIVATHIELERNWLANLGDLYVLFQVPGVQYLLLKQNRFSYCVKHVDAIENNQLIYMDLGENMLQLVWERGLCLDVFRTLSKLQVLHLNNNYLSALPQEIFNGLTSLKRLNLASNLLSHLSLRVFPQSLINLNLSGNQLFSPKPEVFMTLSILDITHNKYVCDCALKSLLVWLNETNVTLAGSESDRYCVYPPALAGVPVSFLTYDDCDEDELQQTLRFSVFVFLSVTLLMFLMSTIIFTRCRGICFVWYKTITKTLIGSHPPAADTSEYMYDAYLCYSKNDFEWVQNSLLKHLDSQYFDKNRFTLCFEERDFLPGEEHINNIRDAIWKSRKTICVVTRQFLKDGWCVEAFNFAQSRYFSDLKEVLIMVVVGSLSQYQLMKHKPIRIFLQRSRYLRWPEDYQDIGWFLDNLSSQILKEKKVQRNVSGIELQTIATVSH

Target ORF information:

RefSeq Version	XM_015283742.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus toll like receptor 5 (TLR5), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015283742.2

ORF Insert Sequence:

ATGATGTTAC ATCAACGGCT AATAATTGTC TTTGGAATAG CACTAGCTGG AGATATATGT 
GCATCTAGAA GCTGCTATTC AGAAGATCAG GTCTCCATGT ATAATTCTTG CAACCTCACA 
GGTGTTCCAC CTGTGCCAAA GGACACTGCA AAGCTTTTTC TAACTTACAA CTATATCAGA 
CAAGTGACTG CAACTTCATT TCCACTGCTG GAGGATTTGT TCTTGTTGGA AATTGGAACA 
CAACGCGTCT TTCCTCTTTA CATAGGAAAA GAAGCTTTCA GGAACCTGCC AAATCTTCGT 
GTCTTGGACC TGGGGTTCAA TAACATTCTT CTGCTGGATC TTGATTCTTT TGCGGGTTTG 
CAACGTTTGA CCATACTCCG TCTGTTTCAG AACAACCTTG GAGATTCCAT CCTGGAGGAG 
CGTTACTTTC AAGACTTGAG ATCATTAGAA GAATTGGATC TTTCAGGAAA CCAAATCACA 
AAACTTCATC CACACCCCTT ATTTTATAAT CTAACCATCT TGAAAGCTGT GAACCTGAAA 
TTCAACAAGA TATCCAACCT GTGTGAAAGT AATCTTACCA GCTTCCAAGG AAAACACTTT 
TCATTCTTTA GCCTCAGTAC TAACACTCTG TATAGGACAG ATAAAATGAT CTGGGCCAAA 
TGCCCAAATC CTTTCAGAAA TATTACATTT AACTCACTGG ACGTTAGTGA GAATGGCTGG 
AGCACAGAGA CAGTCCAGTA CTTCTGCACA GCTATTAAGG GGACTCAAAT CAATTATTTA 
TCATTTCGCT CTCATACAAT GGGCTCAGGA TTTGGCTTTA ATAACTTAAA AAATCCAGAT 
ACTGATACAT TTACAGGACT AGCAAGAAGT GATCTTCATT TGCTTGATAT TTCCAATGGA 
TTCATTTTCT CTCTCAATTC TTTAATCTTT GAAAGTCTTC GTAATCTGGA ATTTCTAAAC 
CTTTTCAGAA ACAAGATAAA TCAAATCCAA AAGCAAGCAT TTTTTGGCTT GGAAAACCTA 
GAAATTCTCA ATCTCTCAAG TAACCTTTTA GGTGAGTTGT ACGATTATAC TTTTGAGGGG 
CTACACAGTA TAATGTATAT TGATTTACAG CAAAATCATA TTGGGATGAT TGGTGAAAAA 
TCTTTCAGTA ACTTAGTAAA TCTGAAAATA ATTGATCTCC GAGACAATGC CATTAAAAAA 
CTCCCTTCCT TCCCACATCT GACTTCTGCC TTTTTAAGTG ACAATAAGCT GATGTCTGTA 
GCTCACACGG CAATAGTAGC AACACATATT GAATTAGAGA GAAACTGGCT CGCAAACCTG 
GGTGACCTGT ATGTTCTTTT CCAAGTTCCA GGTGTGCAGT ATCTCCTCTT AAAACAGAAT 
CGCTTCTCTT ACTGTGTGAA ACATGTTGAT GCTATAGAAA ATAACCAGTT GATTTATATG 
GACCTAGGAG AAAACATGTT GCAGCTTGTG TGGGAGAGAG GTTTATGTTT GGATGTGTTC 
AGGACACTGT CTAAACTTCA GGTTCTACAC CTGAACAACA ACTACCTTAG TGCTCTTCCA 
CAGGAGATTT TTAATGGTCT AACATCTCTA AAAAGACTTA ATCTAGCTTC CAACCTATTG 
TCTCACCTCT CTCTCAGGGT TTTTCCACAA AGCCTAATAA ACCTAAACTT GTCTGGAAAT 
CAGCTTTTTT CCCCTAAGCC TGAAGTCTTT ATGACTTTGA GTATTCTGGA TATAACACAT 
AACAAGTATG TCTGTGACTG TGCTTTAAAA AGCCTATTAG TGTGGCTAAA TGAAACCAAT 
GTAACCCTAG CTGGCTCGGA GTCTGACAGG TACTGTGTGT ACCCACCTGC ATTAGCGGGT 
GTACCCGTGT CATTTCTGAC ATACGATGAC TGCGATGAAG ATGAACTCCA GCAGACACTC 
AGGTTCTCGG TATTCGTTTT CCTATCTGTC ACCCTTCTGA TGTTTCTGAT GTCAACCATT 
ATTTTTACTC GCTGTCGGGG CATTTGTTTT GTCTGGTATA AGACCATCAC CAAAACTTTG 
ATAGGAAGCC ATCCACCTGC AGCAGATACA AGTGAATACA TGTACGACGC ATATTTGTGC 
TACAGCAAAA ATGATTTCGA GTGGGTCCAG AATTCCTTGC TGAAGCACCT GGATTCACAA 
TATTTTGATA AAAACAGATT TACCTTGTGC TTTGAGGAAC GAGATTTCTT GCCTGGGGAA 
GAACATATCA ACAATATTCG TGATGCTATT TGGAAGAGCA GGAAAACAAT TTGTGTTGTG 
ACCAGGCAGT TTCTCAAAGA TGGGTGGTGT GTAGAAGCCT TTAATTTTGC CCAGAGCAGG 
TACTTCAGTG ATTTGAAAGA GGTTCTCATT ATGGTAGTGG TTGGGTCACT TTCTCAATAT 
CAACTGATGA AACACAAACC AATTCGAATT TTTTTACAAA GGAGTCGATA TCTGCGTTGG 
CCTGAAGATT ATCAAGATAT AGGCTGGTTT TTGGATAACC TTTCTTCACA AATTCTGAAG 
GAAAAAAAAG TGCAAAGGAA CGTCAGTGGT ATAGAGCTGC AAACTATAGC GACAGTCTCA 
CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TLR5 ( XM_015283740.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015139226.1 Gallus gallus toll-like receptor 5 (TLR5), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OGa33403
Clone ID Related Accession (Same CDS sequence)	XM_015283741.1 , XM_025148815.1 , XM_015283741.2 , XM_015283742.2 , XM_015283740.1 , XM_015283742.1
Accession Version	XM_015283740.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2586bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	toll-like receptor 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455833.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTTAC ATCAACGGCT AATAATTGTC TTTGGAATAG CACTAGCTGG AGATATATGT 
GCATCTAGAA GCTGCTATTC AGAAGATCAG GTCTCCATGT ATAATTCTTG CAACCTCACA 
GGTGTTCCAC CTGTGCCAAA GGACACTGCA AAGCTTTTTC TAACTTACAA CTATATCAGA 
CAAGTGACTG CAACTTCATT TCCACTGCTG GAGGATTTGT TCTTGTTGGA AATTGGAACA 
CAACGCGTCT TTCCTCTTTA CATAGGAAAA GAAGCTTTCA GGAACCTGCC AAATCTTCGT 
GTCTTGGACC TGGGGTTCAA TAACATTCTT CTGCTGGATC TTGATTCTTT TGCGGGTTTG 
CAACGTTTGA CCATACTCCG TCTGTTTCAG AACAACCTTG GAGATTCCAT CCTGGAGGAG 
CGTTACTTTC AAGACTTGAG ATCATTAGAA GAATTGGATC TTTCAGGAAA CCAAATCACA 
AAACTTCATC CACACCCCTT ATTTTATAAT CTAACCATCT TGAAAGCTGT GAACCTGAAA 
TTCAACAAGA TATCCAACCT GTGTGAAAGT AATCTTACCA GCTTCCAAGG AAAACACTTT 
TCATTCTTTA GCCTCAGTAC TAACACTCTG TATAGGACAG ATAAAATGAT CTGGGCCAAA 
TGCCCAAATC CTTTCAGAAA TATTACATTT AACTCACTGG ACGTTAGTGA GAATGGCTGG 
AGCACAGAGA CAGTCCAGTA CTTCTGCACA GCTATTAAGG GGACTCAAAT CAATTATTTA 
TCATTTCGCT CTCATACAAT GGGCTCAGGA TTTGGCTTTA ATAACTTAAA AAATCCAGAT 
ACTGATACAT TTACAGGACT AGCAAGAAGT GATCTTCATT TGCTTGATAT TTCCAATGGA 
TTCATTTTCT CTCTCAATTC TTTAATCTTT GAAAGTCTTC GTAATCTGGA ATTTCTAAAC 
CTTTTCAGAA ACAAGATAAA TCAAATCCAA AAGCAAGCAT TTTTTGGCTT GGAAAACCTA 
GAAATTCTCA ATCTCTCAAG TAACCTTTTA GGTGAGTTGT ACGATTATAC TTTTGAGGGG 
CTACACAGTA TAATGTATAT TGATTTACAG CAAAATCATA TTGGGATGAT TGGTGAAAAA 
TCTTTCAGTA ACTTAGTAAA TCTGAAAATA ATTGATCTCC GAGACAATGC CATTAAAAAA 
CTCCCTTCCT TCCCACATCT GACTTCTGCC TTTTTAAGTG ACAATAAGCT GATGTCTGTA 
GCTCACACGG CAATAGTAGC AACACATATT GAATTAGAGA GAAACTGGCT CGCAAACCTG 
GGTGACCTGT ATGTTCTTTT CCAAGTTCCA GGTGTGCAGT ATCTCCTCTT AAAACAGAAT 
CGCTTCTCTT ACTGTGTGAA ACATGTTGAT GCTATAGAAA ATAACCAGTT GATTTATATG 
GACCTAGGAG AAAACATGTT GCAGCTTGTG TGGGAGAGAG GTTTATGTTT GGATGTGTTC 
AGGACACTGT CTAAACTTCA GGTTCTACAC CTGAACAACA ACTACCTTAG TGCTCTTCCA 
CAGGAGATTT TTAATGGTCT AACATCTCTA AAAAGACTTA ATCTAGCTTC CAACCTATTG 
TCTCACCTCT CTCTCAGGGT TTTTCCACAA AGCCTAATAA ACCTAAACTT GTCTGGAAAT 
CAGCTTTTTT CCCCTAAGCC TGAAGTCTTT ATGACTTTGA GTATTCTGGA TATAACACAT 
AACAAGTATG TCTGTGACTG TGCTTTAAAA AGCCTATTAG TGTGGCTAAA TGAAACCAAT 
GTAACCCTAG CTGGCTCGGA GTCTGACAGG TACTGTGTGT ACCCACCTGC ATTAGCGGGT 
GTACCCGTGT CATTTCTGAC ATACGATGAC TGCGATGAAG ATGAACTCCA GCAGACACTC 
AGGTTCTCGG TATTCGTTTT CCTATCTGTC ACCCTTCTGA TGTTTCTGAT GTCAACCATT 
ATTTTTACTC GCTGTCGGGG CATTTGTTTT GTCTGGTATA AGACCATCAC CAAAACTTTG 
ATAGGAAGCC ATCCACCTGC AGCAGATACA AGTGAATACA TGTACGACGC ATATTTGTGC 
TACAGCAAAA ATGATTTCGA GTGGGTCCAG AATTCCTTGC TGAAGCACCT GGATTCACAA 
TATTTTGATA AAAACAGATT TACCTTGTGC TTTGAGGAAC GAGATTTCTT GCCTGGGGAA 
GAACATATCA ACAATATTCG TGATGCTATT TGGAAGAGCA GGAAAACAAT TTGTGTTGTG 
ACCAGGCAGT TTCTCAAAGA TGGGTGGTGT GTAGAAGCCT TTAATTTTGC CCAGAGCAGG 
TACTTCAGTG ATTTGAAAGA GGTTCTCATT ATGGTAGTGG TTGGGTCACT TTCTCAATAT 
CAACTGATGA AACACAAACC AATTCGAATT TTTTTACAAA GGAGTCGATA TCTGCGTTGG 
CCTGAAGATT ATCAAGATAT AGGCTGGTTT TTGGATAACC TTTCTTCACA AATTCTGAAG 
GAAAAAAAAG TGCAAAGGAA CGTCAGTGGT ATAGAGCTGC AAACTATAGC GACAGTCTCA 
CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015139226.1
CDS	6579..9164
Translation	MMLHQRLIIVFGIALAGDICASRSCYSEDQVSMYNSCNLTGVPPVPKDTAKLFLTYNYIRQVTATSFPLLEDLFLLEIGTQRVFPLYIGKEAFRNLPNLRVLDLGFNNILLLDLDSFAGLQRLTILRLFQNNLGDSILEERYFQDLRSLEELDLSGNQITKLHPHPLFYNLTILKAVNLKFNKISNLCESNLTSFQGKHFSFFSLSTNTLYRTDKMIWAKCPNPFRNITFNSLDVSENGWSTETVQYFCTAIKGTQINYLSFRSHTMGSGFGFNNLKNPDTDTFTGLARSDLHLLDISNGFIFSLNSLIFESLRNLEFLNLFRNKINQIQKQAFFGLENLEILNLSSNLLGELYDYTFEGLHSIMYIDLQQNHIGMIGEKSFSNLVNLKIIDLRDNAIKKLPSFPHLTSAFLSDNKLMSVAHTAIVATHIELERNWLANLGDLYVLFQVPGVQYLLLKQNRFSYCVKHVDAIENNQLIYMDLGENMLQLVWERGLCLDVFRTLSKLQVLHLNNNYLSALPQEIFNGLTSLKRLNLASNLLSHLSLRVFPQSLINLNLSGNQLFSPKPEVFMTLSILDITHNKYVCDCALKSLLVWLNETNVTLAGSESDRYCVYPPALAGVPVSFLTYDDCDEDELQQTLRFSVFVFLSVTLLMFLMSTIIFTRCRGICFVWYKTITKTLIGSHPPAADTSEYMYDAYLCYSKNDFEWVQNSLLKHLDSQYFDKNRFTLCFEERDFLPGEEHINNIRDAIWKSRKTICVVTRQFLKDGWCVEAFNFAQSRYFSDLKEVLIMVVVGSLSQYQLMKHKPIRIFLQRSRYLRWPEDYQDIGWFLDNLSSQILKEKKVQRNVSGIELQTIATVSH

Target ORF information:

RefSeq Version	XM_015283740.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus toll-like receptor 5 (TLR5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015283740.1

ORF Insert Sequence:

ATGATGTTAC ATCAACGGCT AATAATTGTC TTTGGAATAG CACTAGCTGG AGATATATGT 
GCATCTAGAA GCTGCTATTC AGAAGATCAG GTCTCCATGT ATAATTCTTG CAACCTCACA 
GGTGTTCCAC CTGTGCCAAA GGACACTGCA AAGCTTTTTC TAACTTACAA CTATATCAGA 
CAAGTGACTG CAACTTCATT TCCACTGCTG GAGGATTTGT TCTTGTTGGA AATTGGAACA 
CAACGCGTCT TTCCTCTTTA CATAGGAAAA GAAGCTTTCA GGAACCTGCC AAATCTTCGT 
GTCTTGGACC TGGGGTTCAA TAACATTCTT CTGCTGGATC TTGATTCTTT TGCGGGTTTG 
CAACGTTTGA CCATACTCCG TCTGTTTCAG AACAACCTTG GAGATTCCAT CCTGGAGGAG 
CGTTACTTTC AAGACTTGAG ATCATTAGAA GAATTGGATC TTTCAGGAAA CCAAATCACA 
AAACTTCATC CACACCCCTT ATTTTATAAT CTAACCATCT TGAAAGCTGT GAACCTGAAA 
TTCAACAAGA TATCCAACCT GTGTGAAAGT AATCTTACCA GCTTCCAAGG AAAACACTTT 
TCATTCTTTA GCCTCAGTAC TAACACTCTG TATAGGACAG ATAAAATGAT CTGGGCCAAA 
TGCCCAAATC CTTTCAGAAA TATTACATTT AACTCACTGG ACGTTAGTGA GAATGGCTGG 
AGCACAGAGA CAGTCCAGTA CTTCTGCACA GCTATTAAGG GGACTCAAAT CAATTATTTA 
TCATTTCGCT CTCATACAAT GGGCTCAGGA TTTGGCTTTA ATAACTTAAA AAATCCAGAT 
ACTGATACAT TTACAGGACT AGCAAGAAGT GATCTTCATT TGCTTGATAT TTCCAATGGA 
TTCATTTTCT CTCTCAATTC TTTAATCTTT GAAAGTCTTC GTAATCTGGA ATTTCTAAAC 
CTTTTCAGAA ACAAGATAAA TCAAATCCAA AAGCAAGCAT TTTTTGGCTT GGAAAACCTA 
GAAATTCTCA ATCTCTCAAG TAACCTTTTA GGTGAGTTGT ACGATTATAC TTTTGAGGGG 
CTACACAGTA TAATGTATAT TGATTTACAG CAAAATCATA TTGGGATGAT TGGTGAAAAA 
TCTTTCAGTA ACTTAGTAAA TCTGAAAATA ATTGATCTCC GAGACAATGC CATTAAAAAA 
CTCCCTTCCT TCCCACATCT GACTTCTGCC TTTTTAAGTG ACAATAAGCT GATGTCTGTA 
GCTCACACGG CAATAGTAGC AACACATATT GAATTAGAGA GAAACTGGCT CGCAAACCTG 
GGTGACCTGT ATGTTCTTTT CCAAGTTCCA GGTGTGCAGT ATCTCCTCTT AAAACAGAAT 
CGCTTCTCTT ACTGTGTGAA ACATGTTGAT GCTATAGAAA ATAACCAGTT GATTTATATG 
GACCTAGGAG AAAACATGTT GCAGCTTGTG TGGGAGAGAG GTTTATGTTT GGATGTGTTC 
AGGACACTGT CTAAACTTCA GGTTCTACAC CTGAACAACA ACTACCTTAG TGCTCTTCCA 
CAGGAGATTT TTAATGGTCT AACATCTCTA AAAAGACTTA ATCTAGCTTC CAACCTATTG 
TCTCACCTCT CTCTCAGGGT TTTTCCACAA AGCCTAATAA ACCTAAACTT GTCTGGAAAT 
CAGCTTTTTT CCCCTAAGCC TGAAGTCTTT ATGACTTTGA GTATTCTGGA TATAACACAT 
AACAAGTATG TCTGTGACTG TGCTTTAAAA AGCCTATTAG TGTGGCTAAA TGAAACCAAT 
GTAACCCTAG CTGGCTCGGA GTCTGACAGG TACTGTGTGT ACCCACCTGC ATTAGCGGGT 
GTACCCGTGT CATTTCTGAC ATACGATGAC TGCGATGAAG ATGAACTCCA GCAGACACTC 
AGGTTCTCGG TATTCGTTTT CCTATCTGTC ACCCTTCTGA TGTTTCTGAT GTCAACCATT 
ATTTTTACTC GCTGTCGGGG CATTTGTTTT GTCTGGTATA AGACCATCAC CAAAACTTTG 
ATAGGAAGCC ATCCACCTGC AGCAGATACA AGTGAATACA TGTACGACGC ATATTTGTGC 
TACAGCAAAA ATGATTTCGA GTGGGTCCAG AATTCCTTGC TGAAGCACCT GGATTCACAA 
TATTTTGATA AAAACAGATT TACCTTGTGC TTTGAGGAAC GAGATTTCTT GCCTGGGGAA 
GAACATATCA ACAATATTCG TGATGCTATT TGGAAGAGCA GGAAAACAAT TTGTGTTGTG 
ACCAGGCAGT TTCTCAAAGA TGGGTGGTGT GTAGAAGCCT TTAATTTTGC CCAGAGCAGG 
TACTTCAGTG ATTTGAAAGA GGTTCTCATT ATGGTAGTGG TTGGGTCACT TTCTCAATAT 
CAACTGATGA AACACAAACC AATTCGAATT TTTTTACAAA GGAGTCGATA TCTGCGTTGG 
CCTGAAGATT ATCAAGATAT AGGCTGGTTT TTGGATAACC TTTCTTCACA AATTCTGAAG 
GAAAAAAAAG TGCAAAGGAA CGTCAGTGGT ATAGAGCTGC AAACTATAGC GACAGTCTCA 
CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TLR5 ( XM_015283742.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015139228.1 Gallus gallus toll-like receptor 5 (TLR5), transcript variant X3, mRNA.

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CloneID	OGa33403
Clone ID Related Accession (Same CDS sequence)	XM_015283741.1 , XM_025148815.1 , XM_015283741.2 , XM_015283742.2 , XM_015283740.1 , XM_015283742.1
Accession Version	XM_015283742.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2586bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	toll-like receptor 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455833.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTTAC ATCAACGGCT AATAATTGTC TTTGGAATAG CACTAGCTGG AGATATATGT 
GCATCTAGAA GCTGCTATTC AGAAGATCAG GTCTCCATGT ATAATTCTTG CAACCTCACA 
GGTGTTCCAC CTGTGCCAAA GGACACTGCA AAGCTTTTTC TAACTTACAA CTATATCAGA 
CAAGTGACTG CAACTTCATT TCCACTGCTG GAGGATTTGT TCTTGTTGGA AATTGGAACA 
CAACGCGTCT TTCCTCTTTA CATAGGAAAA GAAGCTTTCA GGAACCTGCC AAATCTTCGT 
GTCTTGGACC TGGGGTTCAA TAACATTCTT CTGCTGGATC TTGATTCTTT TGCGGGTTTG 
CAACGTTTGA CCATACTCCG TCTGTTTCAG AACAACCTTG GAGATTCCAT CCTGGAGGAG 
CGTTACTTTC AAGACTTGAG ATCATTAGAA GAATTGGATC TTTCAGGAAA CCAAATCACA 
AAACTTCATC CACACCCCTT ATTTTATAAT CTAACCATCT TGAAAGCTGT GAACCTGAAA 
TTCAACAAGA TATCCAACCT GTGTGAAAGT AATCTTACCA GCTTCCAAGG AAAACACTTT 
TCATTCTTTA GCCTCAGTAC TAACACTCTG TATAGGACAG ATAAAATGAT CTGGGCCAAA 
TGCCCAAATC CTTTCAGAAA TATTACATTT AACTCACTGG ACGTTAGTGA GAATGGCTGG 
AGCACAGAGA CAGTCCAGTA CTTCTGCACA GCTATTAAGG GGACTCAAAT CAATTATTTA 
TCATTTCGCT CTCATACAAT GGGCTCAGGA TTTGGCTTTA ATAACTTAAA AAATCCAGAT 
ACTGATACAT TTACAGGACT AGCAAGAAGT GATCTTCATT TGCTTGATAT TTCCAATGGA 
TTCATTTTCT CTCTCAATTC TTTAATCTTT GAAAGTCTTC GTAATCTGGA ATTTCTAAAC 
CTTTTCAGAA ACAAGATAAA TCAAATCCAA AAGCAAGCAT TTTTTGGCTT GGAAAACCTA 
GAAATTCTCA ATCTCTCAAG TAACCTTTTA GGTGAGTTGT ACGATTATAC TTTTGAGGGG 
CTACACAGTA TAATGTATAT TGATTTACAG CAAAATCATA TTGGGATGAT TGGTGAAAAA 
TCTTTCAGTA ACTTAGTAAA TCTGAAAATA ATTGATCTCC GAGACAATGC CATTAAAAAA 
CTCCCTTCCT TCCCACATCT GACTTCTGCC TTTTTAAGTG ACAATAAGCT GATGTCTGTA 
GCTCACACGG CAATAGTAGC AACACATATT GAATTAGAGA GAAACTGGCT CGCAAACCTG 
GGTGACCTGT ATGTTCTTTT CCAAGTTCCA GGTGTGCAGT ATCTCCTCTT AAAACAGAAT 
CGCTTCTCTT ACTGTGTGAA ACATGTTGAT GCTATAGAAA ATAACCAGTT GATTTATATG 
GACCTAGGAG AAAACATGTT GCAGCTTGTG TGGGAGAGAG GTTTATGTTT GGATGTGTTC 
AGGACACTGT CTAAACTTCA GGTTCTACAC CTGAACAACA ACTACCTTAG TGCTCTTCCA 
CAGGAGATTT TTAATGGTCT AACATCTCTA AAAAGACTTA ATCTAGCTTC CAACCTATTG 
TCTCACCTCT CTCTCAGGGT TTTTCCACAA AGCCTAATAA ACCTAAACTT GTCTGGAAAT 
CAGCTTTTTT CCCCTAAGCC TGAAGTCTTT ATGACTTTGA GTATTCTGGA TATAACACAT 
AACAAGTATG TCTGTGACTG TGCTTTAAAA AGCCTATTAG TGTGGCTAAA TGAAACCAAT 
GTAACCCTAG CTGGCTCGGA GTCTGACAGG TACTGTGTGT ACCCACCTGC ATTAGCGGGT 
GTACCCGTGT CATTTCTGAC ATACGATGAC TGCGATGAAG ATGAACTCCA GCAGACACTC 
AGGTTCTCGG TATTCGTTTT CCTATCTGTC ACCCTTCTGA TGTTTCTGAT GTCAACCATT 
ATTTTTACTC GCTGTCGGGG CATTTGTTTT GTCTGGTATA AGACCATCAC CAAAACTTTG 
ATAGGAAGCC ATCCACCTGC AGCAGATACA AGTGAATACA TGTACGACGC ATATTTGTGC 
TACAGCAAAA ATGATTTCGA GTGGGTCCAG AATTCCTTGC TGAAGCACCT GGATTCACAA 
TATTTTGATA AAAACAGATT TACCTTGTGC TTTGAGGAAC GAGATTTCTT GCCTGGGGAA 
GAACATATCA ACAATATTCG TGATGCTATT TGGAAGAGCA GGAAAACAAT TTGTGTTGTG 
ACCAGGCAGT TTCTCAAAGA TGGGTGGTGT GTAGAAGCCT TTAATTTTGC CCAGAGCAGG 
TACTTCAGTG ATTTGAAAGA GGTTCTCATT ATGGTAGTGG TTGGGTCACT TTCTCAATAT 
CAACTGATGA AACACAAACC AATTCGAATT TTTTTACAAA GGAGTCGATA TCTGCGTTGG 
CCTGAAGATT ATCAAGATAT AGGCTGGTTT TTGGATAACC TTTCTTCACA AATTCTGAAG 
GAAAAAAAAG TGCAAAGGAA CGTCAGTGGT ATAGAGCTGC AAACTATAGC GACAGTCTCA 
CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015139228.1
CDS	547..3132
Translation	MMLHQRLIIVFGIALAGDICASRSCYSEDQVSMYNSCNLTGVPPVPKDTAKLFLTYNYIRQVTATSFPLLEDLFLLEIGTQRVFPLYIGKEAFRNLPNLRVLDLGFNNILLLDLDSFAGLQRLTILRLFQNNLGDSILEERYFQDLRSLEELDLSGNQITKLHPHPLFYNLTILKAVNLKFNKISNLCESNLTSFQGKHFSFFSLSTNTLYRTDKMIWAKCPNPFRNITFNSLDVSENGWSTETVQYFCTAIKGTQINYLSFRSHTMGSGFGFNNLKNPDTDTFTGLARSDLHLLDISNGFIFSLNSLIFESLRNLEFLNLFRNKINQIQKQAFFGLENLEILNLSSNLLGELYDYTFEGLHSIMYIDLQQNHIGMIGEKSFSNLVNLKIIDLRDNAIKKLPSFPHLTSAFLSDNKLMSVAHTAIVATHIELERNWLANLGDLYVLFQVPGVQYLLLKQNRFSYCVKHVDAIENNQLIYMDLGENMLQLVWERGLCLDVFRTLSKLQVLHLNNNYLSALPQEIFNGLTSLKRLNLASNLLSHLSLRVFPQSLINLNLSGNQLFSPKPEVFMTLSILDITHNKYVCDCALKSLLVWLNETNVTLAGSESDRYCVYPPALAGVPVSFLTYDDCDEDELQQTLRFSVFVFLSVTLLMFLMSTIIFTRCRGICFVWYKTITKTLIGSHPPAADTSEYMYDAYLCYSKNDFEWVQNSLLKHLDSQYFDKNRFTLCFEERDFLPGEEHINNIRDAIWKSRKTICVVTRQFLKDGWCVEAFNFAQSRYFSDLKEVLIMVVVGSLSQYQLMKHKPIRIFLQRSRYLRWPEDYQDIGWFLDNLSSQILKEKKVQRNVSGIELQTIATVSH

Target ORF information:

RefSeq Version	XM_015283742.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus toll-like receptor 5 (TLR5), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015283742.1

ORF Insert Sequence:

ATGATGTTAC ATCAACGGCT AATAATTGTC TTTGGAATAG CACTAGCTGG AGATATATGT 
GCATCTAGAA GCTGCTATTC AGAAGATCAG GTCTCCATGT ATAATTCTTG CAACCTCACA 
GGTGTTCCAC CTGTGCCAAA GGACACTGCA AAGCTTTTTC TAACTTACAA CTATATCAGA 
CAAGTGACTG CAACTTCATT TCCACTGCTG GAGGATTTGT TCTTGTTGGA AATTGGAACA 
CAACGCGTCT TTCCTCTTTA CATAGGAAAA GAAGCTTTCA GGAACCTGCC AAATCTTCGT 
GTCTTGGACC TGGGGTTCAA TAACATTCTT CTGCTGGATC TTGATTCTTT TGCGGGTTTG 
CAACGTTTGA CCATACTCCG TCTGTTTCAG AACAACCTTG GAGATTCCAT CCTGGAGGAG 
CGTTACTTTC AAGACTTGAG ATCATTAGAA GAATTGGATC TTTCAGGAAA CCAAATCACA 
AAACTTCATC CACACCCCTT ATTTTATAAT CTAACCATCT TGAAAGCTGT GAACCTGAAA 
TTCAACAAGA TATCCAACCT GTGTGAAAGT AATCTTACCA GCTTCCAAGG AAAACACTTT 
TCATTCTTTA GCCTCAGTAC TAACACTCTG TATAGGACAG ATAAAATGAT CTGGGCCAAA 
TGCCCAAATC CTTTCAGAAA TATTACATTT AACTCACTGG ACGTTAGTGA GAATGGCTGG 
AGCACAGAGA CAGTCCAGTA CTTCTGCACA GCTATTAAGG GGACTCAAAT CAATTATTTA 
TCATTTCGCT CTCATACAAT GGGCTCAGGA TTTGGCTTTA ATAACTTAAA AAATCCAGAT 
ACTGATACAT TTACAGGACT AGCAAGAAGT GATCTTCATT TGCTTGATAT TTCCAATGGA 
TTCATTTTCT CTCTCAATTC TTTAATCTTT GAAAGTCTTC GTAATCTGGA ATTTCTAAAC 
CTTTTCAGAA ACAAGATAAA TCAAATCCAA AAGCAAGCAT TTTTTGGCTT GGAAAACCTA 
GAAATTCTCA ATCTCTCAAG TAACCTTTTA GGTGAGTTGT ACGATTATAC TTTTGAGGGG 
CTACACAGTA TAATGTATAT TGATTTACAG CAAAATCATA TTGGGATGAT TGGTGAAAAA 
TCTTTCAGTA ACTTAGTAAA TCTGAAAATA ATTGATCTCC GAGACAATGC CATTAAAAAA 
CTCCCTTCCT TCCCACATCT GACTTCTGCC TTTTTAAGTG ACAATAAGCT GATGTCTGTA 
GCTCACACGG CAATAGTAGC AACACATATT GAATTAGAGA GAAACTGGCT CGCAAACCTG 
GGTGACCTGT ATGTTCTTTT CCAAGTTCCA GGTGTGCAGT ATCTCCTCTT AAAACAGAAT 
CGCTTCTCTT ACTGTGTGAA ACATGTTGAT GCTATAGAAA ATAACCAGTT GATTTATATG 
GACCTAGGAG AAAACATGTT GCAGCTTGTG TGGGAGAGAG GTTTATGTTT GGATGTGTTC 
AGGACACTGT CTAAACTTCA GGTTCTACAC CTGAACAACA ACTACCTTAG TGCTCTTCCA 
CAGGAGATTT TTAATGGTCT AACATCTCTA AAAAGACTTA ATCTAGCTTC CAACCTATTG 
TCTCACCTCT CTCTCAGGGT TTTTCCACAA AGCCTAATAA ACCTAAACTT GTCTGGAAAT 
CAGCTTTTTT CCCCTAAGCC TGAAGTCTTT ATGACTTTGA GTATTCTGGA TATAACACAT 
AACAAGTATG TCTGTGACTG TGCTTTAAAA AGCCTATTAG TGTGGCTAAA TGAAACCAAT 
GTAACCCTAG CTGGCTCGGA GTCTGACAGG TACTGTGTGT ACCCACCTGC ATTAGCGGGT 
GTACCCGTGT CATTTCTGAC ATACGATGAC TGCGATGAAG ATGAACTCCA GCAGACACTC 
AGGTTCTCGG TATTCGTTTT CCTATCTGTC ACCCTTCTGA TGTTTCTGAT GTCAACCATT 
ATTTTTACTC GCTGTCGGGG CATTTGTTTT GTCTGGTATA AGACCATCAC CAAAACTTTG 
ATAGGAAGCC ATCCACCTGC AGCAGATACA AGTGAATACA TGTACGACGC ATATTTGTGC 
TACAGCAAAA ATGATTTCGA GTGGGTCCAG AATTCCTTGC TGAAGCACCT GGATTCACAA 
TATTTTGATA AAAACAGATT TACCTTGTGC TTTGAGGAAC GAGATTTCTT GCCTGGGGAA 
GAACATATCA ACAATATTCG TGATGCTATT TGGAAGAGCA GGAAAACAAT TTGTGTTGTG 
ACCAGGCAGT TTCTCAAAGA TGGGTGGTGT GTAGAAGCCT TTAATTTTGC CCAGAGCAGG 
TACTTCAGTG ATTTGAAAGA GGTTCTCATT ATGGTAGTGG TTGGGTCACT TTCTCAATAT 
CAACTGATGA AACACAAACC AATTCGAATT TTTTTACAAA GGAGTCGATA TCTGCGTTGG 
CCTGAAGATT ATCAAGATAT AGGCTGGTTT TTGGATAACC TTTCTTCACA AATTCTGAAG 
GAAAAAAAAG TGCAAAGGAA CGTCAGTGGT ATAGAGCTGC AAACTATAGC GACAGTCTCA 
CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Treatment with ligands for toll-like receptors 2 and 5 induces a mixed T-helper 1- and 2-like response in chicken splenocytes.
Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research32(12)592-8(2012 Dec)
St Paul M,Paolucci S,Sharif S

Different genetic patterns in avian Toll-like receptor (TLR)5 genes.
Molecular biology reports39(4)3419-26(2012 Apr)
Ruan W,Wu Y,Zheng SJ

Toll-like receptor mRNA expression, iNOS gene polymorphism and serum nitric oxide levels in indigenous chickens.
Veterinary research communications35(5)321-7(2011 Jun)
Ramasamy KT,Reddy MR,Murugesan S

Functional characterization of chicken TLR5 reveals species-specific recognition of flagellin.
Molecular immunology45(5)1298-307(2008 Mar)
Keestra AM,de Zoete MR,van Aubel RA,van Putten JP

Identification and functional characterization of chicken toll-like receptor 5 reveals a fundamental role in the biology of infection with Salmonella enterica serovar typhimurium.
Infection and immunity73(4)2344-50(2005 Apr)
Iqbal M,Philbin VJ,Withanage GS,Wigley P,Beal RK,Goodchild MJ,Barrow P,McConnell I,Maskell DJ,Young J,Bumstead N,Boyd Y,Smith AL

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TLR5 cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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