SELENOI cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	SELENOI
Entrez Gene ID	428661
Full Name	selenoprotein I
Synonyms	EPT1,SELI
General protein information	Preferred Names selenoprotein I Names ethanolaminephosphotransferase 1 ethanolaminephosphotransferase 1 (CDP-ethanolamine-specific)
Gene Type	protein-coding
Organism	Gallus gallus(chicken)
Summary	The multi-pass transmembrane protein encoded by this gene belongs to the CDP-alcohol phosphatidyltransferase class-I family. It catalyzes the transfer of phosphoethanolamine from CDP-ethanolamine to diacylglycerol to produce phosphatidylethanolamine, which is involved in the formation and maintenance of vesicular membranes, regulation of lipid metabolism, and protein folding. This protein is a selenoprotein, containing the rare selenocysteine (Sec) amino acid at its active site. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. [provided by RefSeq, Jul 2016].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001031528.3	NP_001026699.2	ethanolaminephosphotransferase 1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	EPT1	NP_001140169.1
	EPT1	NP_001129035.1
Gallus gallus (chicken)	EPT1	NP_001026699.2
Caenorhabditis elegans (roundworm)	CELE_F54D7.2	NP_491454.2
Mus musculus (house mouse)	Ept1	NP_081928.2
Rattus norvegicus (Norway rat)	Seli	NP_001128226.2
Drosophila melanogaster (fruit fly)	CG33116	NP_788074.1
Xenopus tropicalis (tropical clawed frog)	ept1	NP_001004832.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008881	XP_319628.3
Homo sapiens (human)	EPT1	NP_277040.1
Canis lupus familiaris (dog)	EPT1	NP_001157958.1
Bos taurus (cattle)	EPT1	NP_001068725.2

Gene Ontology
Bibliography

Related articles in PubMed

Chicken genomics resource: sequencing and annotation of 35,407 ESTs from single and multiple tissue cDNA libraries and CAP3 assembly of a chicken gene index.
Carre W, Wang X, Porter TE, Nys Y, Tang J, Bernberg E, Morgan R, Burnside J, Aggrey SE, Simon J, Cogburn LA
Physiological genomics25(3)514-24(2006 May)

Full-length cDNAs from chicken bursal lymphocytes to facilitate gene function analysis.
Caldwell RB, Kierzek AM, Arakawa H, Bezzubov Y, Zaim J, Fiedler P, Kutter S, Blagodatski A, Kostovska D, Koter M, Plachy J, Carninci P, Hayashizaki Y, Buerstedde JM
Genome biology6(1)R6(2005)

A comprehensive collection of chicken cDNAs.
Boardman PE, Sanz-Ezquerro J, Overton IM, Burt DW, Bosch E, Fong WT, Tickle C, Brown WR, Wilson SA, Hubbard SJ
Current biology : CB12(22)1965-9(2002 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following SELENOI gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SELENOI cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OGa26439	NM_001031528.3 Latest version!	Gallus gallus selenoprotein I (SELENOI), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SELENOI ( NM_001031528.3 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_001026699.2 Gallus gallus selenoprotein I (SELENOI), mRNA.

Price & Availability↑

CloneID	OGa26439
Clone ID Related Accession (Same CDS sequence)	NM_001031528.3
Accession Version	NM_001031528.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1203bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-17
Organism	Gallus gallus(chicken)
Product	ethanolaminephosphotransferase 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AJ719721.1, BU259511.1, BU298089.1, AJ732966.1, BM490310.1 and AADN05000843.1. On Jul 8, 2016 this sequence version replaced NM_001031528.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AJ719721.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2201358, SAMEA2201359 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## protein contains selenocysteine :: inferred from conservation ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGAGTACG TGACGGCCGA GCAGCTGGCC GGCTTCAGCA AGTATAAGTA CAGTGCCGTG 
GACAGCAACC CTCTGTCTCT GTACGTCATG CATCCCTTCT GGAACACCAT AGTGAAGATC 
TTCCCTACGT GGCTGGCCCC AAATCTCATA ACGTTTTCTG GCTTCCTGCT GCTTGTCTTC 
AACTTCTTCC TCATGGCATA CTTTGACCCT GACTTTTACG CTTCTGCTCC TGACCACCAG 
CACGTTCCAA ATGGAGTATG GGTCGTTGTG GGTCTCCTCA ACTTCATTGC CTACACGTTA 
GATGGTGTTG ATGGCAAACA AGCTCGCAGG ACCAACTCCA GCACACCCCT AGGAGAGCTT 
TTTGACCATG GCCTGGACAG CTGGGCTTGT GTGTACTTCG TTGTGACTGT CTACTCCACC 
TTTGGCCGGG GCTCCACGGG TGTCAGTGTC TTCGTTCTCT ACCTCCTCTT ATGGGTGGTG 
TTGTTTTCAT TCATCCTCTC CCATTGGGAG AAGTATAACA CAGGGATTCT CTTCCTGCCC 
TGGGGATATG ACATCAGCCA GGTGACCATT TCAATTGTCT ACATAGTGAC AGCCATTGTG 
GGAGTAGAGG CCTGGTATGC ACCTTTCCTG TTTAATTTCT TATATAGAGA CCTATTCACT 
ACAATGATTA TTGCTTGTGC TCTCACCGTG ACGCTGCCGA TGAGCCTGTA TAACTTCTAC 
AAGGCCTATA AAAATAATAC CTTGAAGCAC CACTCTGTTT ACGAGATCAT GCTGCCACTG 
GTCTCCCCAG TGTTGCTTTT TGCTCTCTGC ACCACTTGGA TCTTTGTTTC ACCTATGGAC 
ATCCTGGAGG TCCATCCCAG GCTCTTCTAT TTCATGGTTG GAACCGCCTT CGCTAACATT 
TCTTGCCAGC TGATCGTCTG TCAGATGAGC AGCACTCGCT GCCAGCCTCT GAACTGGATG 
CTGCTGCCCA TAGCCCTGGT GCTCTTCATG GTGATGTCTG GGTTTGCACC CAGCAGCGAA 
ACGCTTCTCC TCTACCTGCT AACTGCATTC CTCACCCTGG CGCACATCCA CTATGGAGTG 
GTCGTGGTGA GCCAACTGAG CAGGCACTTC AATATACGAC CCTTCTCACT AAAGAAACCC 
ACGCCGGATT GACTAGGAAT GGAGGAAGAG AAAATCAGCT TGCGGTCTGC AGAAGTACTG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001026699.2
CDS	108..1310
Translation	MEYVTAEQLAGFSKYKYSAVDSNPLSLYVMHPFWNTIVKIFPTWLAPNLITFSGFLLLVFNFFLMAYFDPDFYASAPDHQHVPNGVWVVVGLLNFIAYTLDGVDGKQARRTNSSTPLGELFDHGLDSWACVYFVVTVYSTFGRGSTGVSVFVLYLLLWVVLFSFILSHWEKYNTGILFLPWGYDISQVTISIVYIVTAIVGVEAWYAPFLFNFLYRDLFTTMIIACALTVTLPMSLYNFYKAYKNNTLKHHSVYEIMLPLVSPVLLFALCTTWIFVSPMDILEVHPRLFYFMVGTAFANISCQLIVCQMSSTRCQPLNWMLLPIALVLFMVMSGFAPSSETLLLYLLTAFLTLAHIHYGVVVVSQLSRHFNIRPFSLKKPTPD*LGMEEEKISLRSAEVL

Target ORF information:

RefSeq Version	NM_001031528.3
Organism	Gallus gallus(chicken)
Definition	Gallus gallus selenoprotein I (SELENOI), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001031528.3

ORF Insert Sequence:

ATGGAGTACG TGACGGCCGA GCAGCTGGCC GGCTTCAGCA AGTATAAGTA CAGTGCCGTG 
GACAGCAACC CTCTGTCTCT GTACGTCATG CATCCCTTCT GGAACACCAT AGTGAAGATC 
TTCCCTACGT GGCTGGCCCC AAATCTCATA ACGTTTTCTG GCTTCCTGCT GCTTGTCTTC 
AACTTCTTCC TCATGGCATA CTTTGACCCT GACTTTTACG CTTCTGCTCC TGACCACCAG 
CACGTTCCAA ATGGAGTATG GGTCGTTGTG GGTCTCCTCA ACTTCATTGC CTACACGTTA 
GATGGTGTTG ATGGCAAACA AGCTCGCAGG ACCAACTCCA GCACACCCCT AGGAGAGCTT 
TTTGACCATG GCCTGGACAG CTGGGCTTGT GTGTACTTCG TTGTGACTGT CTACTCCACC 
TTTGGCCGGG GCTCCACGGG TGTCAGTGTC TTCGTTCTCT ACCTCCTCTT ATGGGTGGTG 
TTGTTTTCAT TCATCCTCTC CCATTGGGAG AAGTATAACA CAGGGATTCT CTTCCTGCCC 
TGGGGATATG ACATCAGCCA GGTGACCATT TCAATTGTCT ACATAGTGAC AGCCATTGTG 
GGAGTAGAGG CCTGGTATGC ACCTTTCCTG TTTAATTTCT TATATAGAGA CCTATTCACT 
ACAATGATTA TTGCTTGTGC TCTCACCGTG ACGCTGCCGA TGAGCCTGTA TAACTTCTAC 
AAGGCCTATA AAAATAATAC CTTGAAGCAC CACTCTGTTT ACGAGATCAT GCTGCCACTG 
GTCTCCCCAG TGTTGCTTTT TGCTCTCTGC ACCACTTGGA TCTTTGTTTC ACCTATGGAC 
ATCCTGGAGG TCCATCCCAG GCTCTTCTAT TTCATGGTTG GAACCGCCTT CGCTAACATT 
TCTTGCCAGC TGATCGTCTG TCAGATGAGC AGCACTCGCT GCCAGCCTCT GAACTGGATG 
CTGCTGCCCA TAGCCCTGGT GCTCTTCATG GTGATGTCTG GGTTTGCACC CAGCAGCGAA 
ACGCTTCTCC TCTACCTGCT AACTGCATTC CTCACCCTGG CGCACATCCA CTATGGAGTG 
GTCGTGGTGA GCCAACTGAG CAGGCACTTC AATATACGAC CCTTCTCACT AAAGAAACCC 
ACGCCGGATT GACTAGGAAT GGAGGAAGAG AAAATCAGCT TGCGGTCTGC AGAAGTACTG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Chicken genomics resource: sequencing and annotation of 35,407 ESTs from single and multiple tissue cDNA libraries and CAP3 assembly of a chicken gene index.
Physiological genomics25(3)514-24(2006 May)
Carre W,Wang X,Porter TE,Nys Y,Tang J,Bernberg E,Morgan R,Burnside J,Aggrey SE,Simon J,Cogburn LA

Full-length cDNAs from chicken bursal lymphocytes to facilitate gene function analysis.
Genome biology6(1)R6(2005)
Caldwell RB,Kierzek AM,Arakawa H,Bezzubov Y,Zaim J,Fiedler P,Kutter S,Blagodatski A,Kostovska D,Koter M,Plachy J,Carninci P,Hayashizaki Y,Buerstedde JM

A comprehensive collection of chicken cDNAs.
Current biology : CB12(22)1965-9(2002 Nov)
Boardman PE,Sanz-Ezquerro J,Overton IM,Burt DW,Bosch E,Fong WT,Tickle C,Brown WR,Wilson SA,Hubbard SJ

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SELENOI cDNA ORF clone, Gallus gallus(chicken)

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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